LOCUS       DACCXW010000001        75218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCXW010000001 DACCXW010000000
VERSION     DACCXW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.001"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1461)
                     /locus_tag="IVY71_000001"
     CDS             complement(<1..1461)
                     /locus_tag="IVY71_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7984085.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHL"
     gene            complement(1759..2694)
                     /gene="murB"
                     /locus_tag="IVY71_000002"
     CDS             complement(1759..2694)
                     /gene="murB"
                     /locus_tag="IVY71_000002"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7984086.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2767..3687)
                     /locus_tag="IVY71_000003"
     CDS             complement(2767..3687)
                     /locus_tag="IVY71_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7984087.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3800..4552
                     /gene="xth"
                     /locus_tag="IVY71_000004"
     CDS             3800..4552
                     /gene="xth"
                     /locus_tag="IVY71_000004"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7984088.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5416..5949
                     /locus_tag="IVY71_000005"
     CDS             5416..5949
                     /locus_tag="IVY71_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7984089.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6439..6963)
                     /locus_tag="IVY71_000006"
     CDS             complement(6439..6963)
                     /locus_tag="IVY71_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7984090.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(6956..7429)
                     /gene="tsaE"
                     /locus_tag="IVY71_000007"
     CDS             complement(6956..7429)
                     /gene="tsaE"
                     /locus_tag="IVY71_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7984091.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7552..8532)
                     /gene="pta"
                     /locus_tag="IVY71_000008"
     CDS             complement(7552..8532)
                     /gene="pta"
                     /locus_tag="IVY71_000008"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7984092.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8685..9365)
                     /locus_tag="IVY71_000009"
     CDS             complement(8685..9365)
                     /locus_tag="IVY71_000009"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7984093.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9491..10360
                     /locus_tag="IVY71_000010"
     CDS             9491..10360
                     /locus_tag="IVY71_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7984094.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10386..10901
                     /locus_tag="IVY71_000011"
     CDS             10386..10901
                     /locus_tag="IVY71_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984095.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(10948..11511)
                     /locus_tag="IVY71_000012"
     CDS             complement(10948..11511)
                     /locus_tag="IVY71_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7984096.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11566..11745)
                     /locus_tag="IVY71_000013"
     CDS             complement(11566..11745)
                     /locus_tag="IVY71_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984097.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11822..12331)
                     /locus_tag="IVY71_000014"
     CDS             complement(11822..12331)
                     /locus_tag="IVY71_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984098.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12427..12807)
                     /locus_tag="IVY71_000015"
     CDS             complement(12427..12807)
                     /locus_tag="IVY71_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7984099.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13137..13886
                     /locus_tag="IVY71_000016"
     CDS             13137..13886
                     /locus_tag="IVY71_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7984100.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14070..15347)
                     /locus_tag="IVY71_000017"
     CDS             complement(14070..15347)
                     /locus_tag="IVY71_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984101.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15426..16070)
                     /gene="pcp"
                     /locus_tag="IVY71_000018"
     CDS             complement(15426..16070)
                     /gene="pcp"
                     /locus_tag="IVY71_000018"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7984102.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16269..17213)
                     /locus_tag="IVY71_000019"
     CDS             complement(16269..17213)
                     /locus_tag="IVY71_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7984103.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17215..17901)
                     /locus_tag="IVY71_000020"
     CDS             complement(17215..17901)
                     /locus_tag="IVY71_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7984104.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18375..18695
                     /locus_tag="IVY71_000021"
     CDS             18375..18695
                     /locus_tag="IVY71_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7984105.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18849..19754
                     /locus_tag="IVY71_000022"
     CDS             18849..19754
                     /locus_tag="IVY71_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7984106.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19751..20524
                     /locus_tag="IVY71_000023"
     CDS             19751..20524
                     /locus_tag="IVY71_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7984107.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20705..22129)
                     /locus_tag="IVY71_000024"
     CDS             complement(20705..22129)
                     /locus_tag="IVY71_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7984108.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22340..22786
                     /gene="nrdI"
                     /locus_tag="IVY71_000025"
     CDS             22340..22786
                     /gene="nrdI"
                     /locus_tag="IVY71_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7984109.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22851..24833)
                     /locus_tag="IVY71_000026"
     CDS             complement(22851..24833)
                     /locus_tag="IVY71_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7984110.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24847..25743)
                     /gene="murQ"
                     /locus_tag="IVY71_000027"
     CDS             complement(24847..25743)
                     /gene="murQ"
                     /locus_tag="IVY71_000027"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7984111.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25746..26807)
                     /locus_tag="IVY71_000028"
     CDS             complement(25746..26807)
                     /locus_tag="IVY71_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7984112.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(27018..27545)
                     /locus_tag="IVY71_000029"
     CDS             complement(27018..27545)
                     /locus_tag="IVY71_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7984113.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(27612..29114)
                     /locus_tag="IVY71_000030"
     CDS             complement(27612..29114)
                     /locus_tag="IVY71_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7984114.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29372..30493)
                     /locus_tag="IVY71_000031"
     CDS             complement(29372..30493)
                     /locus_tag="IVY71_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7984115.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30619..31560
                     /locus_tag="IVY71_000032"
     CDS             30619..31560
                     /locus_tag="IVY71_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7984116.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31710..32606)
                     /locus_tag="IVY71_000033"
     CDS             complement(31710..32606)
                     /locus_tag="IVY71_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7984117.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            32875..34215
                     /locus_tag="IVY71_000034"
     CDS             32875..34215
                     /locus_tag="IVY71_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7984118.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            34433..34876
                     /locus_tag="IVY71_000035"
     CDS             34433..34876
                     /locus_tag="IVY71_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7984119.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(35380..36798)
                     /gene="nhaC"
                     /locus_tag="IVY71_000036"
     CDS             complement(35380..36798)
                     /gene="nhaC"
                     /locus_tag="IVY71_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7984120.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(37201..38424)
                     /locus_tag="IVY71_000037"
     CDS             complement(37201..38424)
                     /locus_tag="IVY71_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7984121.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(38455..39294)
                     /locus_tag="IVY71_000038"
     CDS             complement(38455..39294)
                     /locus_tag="IVY71_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7984122.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(39311..39991)
                     /locus_tag="IVY71_000039"
     CDS             complement(39311..39991)
                     /locus_tag="IVY71_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984123.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(39993..41066)
                     /locus_tag="IVY71_000040"
     CDS             complement(39993..41066)
                     /locus_tag="IVY71_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7984124.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(41713..42384)
                     /locus_tag="IVY71_000041"
     CDS             complement(41713..42384)
                     /locus_tag="IVY71_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7984125.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(42471..45149)
                     /gene="mgtA"
                     /locus_tag="IVY71_000042"
     CDS             complement(42471..45149)
                     /gene="mgtA"
                     /locus_tag="IVY71_000042"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7984126.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            45808..46245
                     /gene="ndk"
                     /locus_tag="IVY71_000043"
     CDS             45808..46245
                     /gene="ndk"
                     /locus_tag="IVY71_000043"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7984127.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(46267..47133)
                     /locus_tag="IVY71_000044"
     CDS             complement(46267..47133)
                     /locus_tag="IVY71_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7984128.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(47293..49386)
                     /locus_tag="IVY71_000045"
     CDS             complement(47293..49386)
                     /locus_tag="IVY71_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7984129.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(50135..50758)
                     /locus_tag="IVY71_000046"
     CDS             complement(50135..50758)
                     /locus_tag="IVY71_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984130.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(50917..52314)
                     /locus_tag="IVY71_000047"
     CDS             complement(50917..52314)
                     /locus_tag="IVY71_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7984131.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(52346..53317)
                     /locus_tag="IVY71_000048"
     CDS             complement(52346..53317)
                     /locus_tag="IVY71_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7984132.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            53691..54116
                     /locus_tag="IVY71_000049"
     CDS             53691..54116
                     /locus_tag="IVY71_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984133.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(54604..55305)
                     /locus_tag="IVY71_000050"
     CDS             complement(54604..55305)
                     /locus_tag="IVY71_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7984134.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(55321..55761)
                     /locus_tag="IVY71_000051"
     CDS             complement(55321..55761)
                     /locus_tag="IVY71_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7984135.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(55761..56336)
                     /locus_tag="IVY71_000052"
     CDS             complement(55761..56336)
                     /locus_tag="IVY71_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7984136.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(57184..57663)
                     /locus_tag="IVY71_000053"
                     /pseudo
     CDS             complement(57184..57663)
                     /locus_tag="IVY71_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(57822..58523)
                     /locus_tag="IVY71_000054"
     CDS             complement(57822..58523)
                     /locus_tag="IVY71_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7984137.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(58631..60040)
                     /locus_tag="IVY71_000055"
     CDS             complement(58631..60040)
                     /locus_tag="IVY71_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7984138.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(60088..61953)
                     /locus_tag="IVY71_000056"
     CDS             complement(60088..61953)
                     /locus_tag="IVY71_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7984139.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(62074..62924)
                     /locus_tag="IVY71_000057"
                     /pseudo
     CDS             complement(62074..62924)
                     /locus_tag="IVY71_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(63234..63947)
                     /locus_tag="IVY71_000058"
     CDS             complement(63234..63947)
                     /locus_tag="IVY71_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7984140.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(64015..64341)
                     /locus_tag="IVY71_000059"
     CDS             complement(64015..64341)
                     /locus_tag="IVY71_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7984141.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(64464..64691)
                     /locus_tag="IVY71_000060"
     CDS             complement(64464..64691)
                     /locus_tag="IVY71_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7984142.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(65013..66266)
                     /locus_tag="IVY71_000061"
     CDS             complement(65013..66266)
                     /locus_tag="IVY71_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7984143.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            66327..67067
                     /locus_tag="IVY71_000062"
     CDS             66327..67067
                     /locus_tag="IVY71_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7984144.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(67153..67911)
                     /locus_tag="IVY71_000063"
     CDS             complement(67153..67911)
                     /locus_tag="IVY71_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7984145.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(67908..68936)
                     /locus_tag="IVY71_000064"
                     /pseudo
     CDS             complement(67908..68936)
                     /locus_tag="IVY71_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(68956..72123)
                     /locus_tag="IVY71_000065"
     CDS             complement(68956..72123)
                     /locus_tag="IVY71_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984146.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(72347..73231)
                     /locus_tag="IVY71_000066"
     CDS             complement(72347..73231)
                     /locus_tag="IVY71_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7984147.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(73326..73763)
                     /locus_tag="IVY71_000067"
     CDS             complement(73326..73763)
                     /locus_tag="IVY71_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984148.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(73980..74891)
                     /locus_tag="IVY71_000068"
     CDS             complement(73980..74891)
                     /locus_tag="IVY71_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7984149.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT        21657 a        15683 c        12931 g        24947 t
ORIGIN      
        1 taaatgtcta tttttttctc tgatgatggt atttccaaaa tattcccagt catctggagt
       61 cggtggattt ttccaaatta tctgttcttt attaaatttt tcaagtaaac aatcagataa
      121 ataaatattc gtacgagaag tgattcgact ctcaatcaca atattgtaat ttttaaatcc
      181 tttcacttga gaaataatgt aatcggtata agcagatcct tgcgaaaaat caatacagac
      241 atgaatcggt gcttcaaaat atgataccgg acaaataccg accaataaaa agagataatc
      301 ataaaataag cgaatcatga aacgttcatc ttttcccaag aaatagcgtt tattctcttg
      361 tatgaccttt tggattgctg tactgaatat tggatacgtc tcgaagaaaa actgtagctg
      421 ctttttagac gtaaagctag tcatctcaaa gtcgaatatc caattttttc gattgacatg
      481 catcataccc atacgaagtt cttttcgtat atatagtctt tcttcttcag agatttcaat
      541 cgaaaatggt aattgatcaa tcaattccac tgacattttt tgactaatca tgtggatatc
      601 atgaaaatga gcttccgcaa gtccaaatgc gccatctaat tcaaattctt cttccgtgat
      661 caaaaacaac atgatataag aaatctcatc aatgatctgc tctttaggga tatgaagaaa
      721 agaaatatgt gataaatcct tgatgatctt cattccttct gagctcttaa agtattcatc
      781 tgaaaagaaa cgttttccga ttggttgatt cccataggcc ctcaacaaac taatagtggt
      841 tagcaaattg aatttgattc gttttgttgc agacagtttt aaattgaaca gatattcaaa
      901 atattgcacg atactctttg cttcattcat gacgctttct ctaaatggca tagaaaaacc
      961 attaaagacc ccgaaatata ccccaaataa tacgttccgt atgtttattt cttccccaac
     1021 taatatgttt ttctttaact ttatttgatg ctgttcaaga aaagagacta attctcttct
     1081 ttttacatag gctcttgata tgcttaaaaa atgattctct gcaaattttt cgatcgttac
     1141 ttttgaatcc acgatctccg ttaacagtcg agcaattttt gatcgttgaa agtagattct
     1201 tcttaaattt ttgatatccg tcaaattcag acgttcgaca tggatctctg cttcttctga
     1261 aatttggatc tctggctttg ttttgaattc agtaaattct ttatttaatt ctatgatata
     1321 atttcttgtt ttaaatttag acaaacctaa tgtttcatat aacttaggca gcactacata
     1381 attctttttc tcaaattcta aatatctagc tagcagcagt ttttctctat cttcctcttc
     1441 tataaggtaa tccaacttca tatcctacac ctcaaaaaat gcctcatacc aattacttga
     1501 tatgcagatg aaagttcatc ttttcaccta tactgatttt atgatggatt atgcttgttt
     1561 tttcatcagt tattctcatt ttttatcttt tttttgagaa tattgcaaaa aatggaaaaa
     1621 tttatgagga aatattctat attttttgtt ttattgtgtt aaaatgtaca agatttgcgc
     1681 catttgcgta aacaaccaca aaaaagagca ggacggccac cgtcctgctc ttttacttat
     1741 tttaagttcc attacttatt aattggctaa acttctgctt gtttctgata ttgcttgttt
     1801 ttctccaatg atccgcactt ctgtttctaa agacacatca aatttttctt tgattacttc
     1861 ttggatgtgc gcaatcaatt caatataatc tgtcgctgtc gcatgatcga tattgacgat
     1921 gaatccagca tgtttttcag agacttgtgc accgccccat ttcaggcctt gcaatcctgc
     1981 ttgttggatc agctgacctg tatagtgtcc tactgggcgc ttaaagacac ttccgcatga
     2041 agggtattct aatggctgtt ttgattgacg caattcggtc aactcatcca ttcttgcttt
     2101 gattgcttca taatcccctg tttgcaaact gaatcgagct tccaatacga tagcacgtaa
     2161 ttcttggact ttactgtgac gataagaaaa taccatttcg tcttttgtca aagttttcaa
     2221 tgttccatct tcaagcaaaa gatccacttc agcaaaaaca tctttgatct ctccgccata
     2281 cgcacctgca ttcatataaa ccgctccacc gacacttccc gggatgccgc aagcaaattc
     2341 aaatccagtc aatgattctg ccaaagctgc ataagtcgta tcaatcagtg aggcacctgc
     2401 ttccgcaatg accatcgttc ctttgacatg aatctgtttc atttcagtca gcatgatgac
     2461 tacaccgcga atcccgccat cttgaacgat cagattgctt gcatttccta ataccatcca
     2521 aggtatatca tggattcggc aataatcgat gatttgcttt acttcttctt gttttttcgg
     2581 gaatgcaagg acatcagctg gtccacctgt tttcgtaaac gtatagttca tcaatggttc
     2641 atcaaacaat atagtaatcg tggctaagct ttcaactatt tcttctttat tcactgagga
     2701 ttttcctttc ttttctcaaa cgtcctcatt attctatcac gcttaataca atcttgccag
     2761 tcttgttcaa agttgatgac ggtagtgatc tttaagcact tcctcacata attcatgact
     2821 tcttacaact tctttttgtt taagcaattc tgtttccgat ttacctaaac ggatctgaga
     2881 aaagaaggcc tctaccattg gctcaaaccc gcgtttgact agcgtgtttg tccagtcgcc
     2941 aaattctttc actttttttc catcagcatc ttgaatgaca agctgggtca gatcgtttac
     3001 ttcataagta ccatttttac ttgttacttg atatttttct atattagcgc cacttgctaa
     3061 atccatcgtt aaaatagccg tttgattttg tgtttctaat tgtaaaatgg catattccaa
     3121 aaattccttt gtttctctta tctggtattt tgttctcaaa atgggttcat ctaataaata
     3181 cacagctgta tcaataagat gtaaaaacaa atcataaagg acgaactctg tcgtttcttt
     3241 tgccgctatt cgattttttt gcaggtggat caaccgctta tctgggatac gtttcaattc
     3301 ttctaccata ggagcaaagc gccggttgaa accgatcatc aaaattattt ggttcttatc
     3361 agctaattct tgcaattcac gaacttcatt tagatggaca ctcagtggtt tgtcaatata
     3421 aacatggata ccattttcta gacatttttt ggctaattca taatgtactt ttgttgctgc
     3481 gtggatcata catgcttcga ttttttcttc taaaagctca tcgatcgttt catatagatg
     3541 ttggaacccg tactcttttt gaattctttc ttttgtttct ttatttcttg ttgcaaaata
     3601 aaaatcaagt gttccttgtt ttttcgcata agttggtaaa tatgcttttt ctgcgatatt
     3661 ccctacaccg attacaccaa tcttcatcta ctttcctcct ggatcattta gttactttca
     3721 tcttacacca tttttttcta aaatgaggta caatagtatc gcaaaggtaa ggaaattaat
     3781 tcgaaggagt gtgcttttat tgaaatttat ctcttggaac gtaaacgggc ttcgtgccat
     3841 cgtaaataaa aattttttag aagtttttca tgagttagac gccgattttt tctgtctgca
     3901 ggaaacgaaa ctccaagcag gacaaattga tttagatctt ccaggttatt atcaatattg
     3961 gaattacgcg gaacgaaaag gctattccgg cacagcgatt tttgctaaaa aaccagcatt
     4021 gaatgccact tacggaatgg gtatcgatat ccatgataca gaaggccgtc tgattacttt
     4081 ggaatattcc gatttctttc ttgtaacctg ttacacgcca aattctcaaa gcgaattaaa
     4141 acgtttagat taccgattag aatgggaaga agccttttat aattatttgg aaaatctaaa
     4201 aaaacaaaaa ccagtcatcg tgtgtgggga tctaaatgtc gctcatcaaa agattgattt
     4261 gaaaaactgg aaaacgaatc aaaaaaatgc tggattcact ccagaagaaa gagcggctct
     4321 ttctcgactg ttggataacg ggtttatcga tacattccgt tatttttatc caacccagga
     4381 aggtgtctac tcttggtgga actaccgatt caattcaagg aaaaacaatg ctggttggcg
     4441 gatcgattac tttttgacga gcaaagattt agagccagga ctagctgatg caaagatcca
     4501 tacggacatt atgggcagtg atcattgtcc agtagaatta gatttgaaat aaagaggttg
     4561 tgaaaaagtc gtttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt
     4621 ttgcataaat ttggcataat accgagaaac tggcttttga acactgttta tcgagagatt
     4681 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
     4741 ttttcgtttg gttgggctta ttaccgcaga tacaagcttt tgaacaccgt ttattaagag
     4801 gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4861 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa
     4921 gaggttgtga aaaagctgca ctgcatcaag taataagaac agccaaacaa aaatagttcc
     4981 tcatattttt cgtttggttg gacttattac cgcagatgca agcttttgaa caccgtttat
     5041 tgagaagttg tgaaatcagc gtcctgacct gagtattaga gaaacaaatg gcaaaatagt
     5101 tcctcatatt ttgctatttt ttgagctaat accgagggtc agctgattga acaccgttta
     5161 gtggagaagt tgtgaaaaag tcgcctactt tcgagtcgta agaaattttt ttgaaaatgg
     5221 ttcctcacaa ctttgcataa atttgactta ataccgagaa gtcatgatcc ccatctaact
     5281 ccaaatcaca aggaatgagt tcaaaaacta gcttttctca acaaatatta cataaaaaat
     5341 tctatgtttt ttgttaaatt tatttgcttt tttcaaaagt atgttacagt aatttcccaa
     5401 ggaggtttta cagaaatgaa ttttgttgcc atggattttg aaactgccaa tcatcagcct
     5461 tatagtgctt gttctcttgc gctagtcatg gtaaaaaata gccagatcgt tgatgaattc
     5521 tatacattga tacagcctga gactcctttt ttctggcgaa atgtccaaat tcacggaatc
     5581 catcaagaag acgtacgcaa tgcgccgaaa ttcccagaag tctggcaaac tatccaacca
     5641 tattttcaaa aaaatcgttt agtcgttgca cataatgctg cttttgatac aaaggtctta
     5701 gcgggatgct tagattatta tcatcttgaa cagcctaatt atctttcttt atgtacagtg
     5761 aaaagtagcc gtcgcctgtt tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa
     5821 aatttgaaca tccaattaga tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg
     5881 gaaatcctgc tttatcaaga aaaaaaattc gggacagatc cattgaaaaa acttgtgact
     5941 ataaagtaaa agaatactta tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc
     6001 gagtcacaag gagcaatttc aaaaaatagc ttctcatgtt tattaaatat tgtgactggt
     6061 gaccgaggag ctggcaggag aacaccgttt attggagagg ttgtgaaaaa gctgttctgc
     6121 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttggact
     6181 tattaccgca gatgcaagct tttggacacc gtttaccaag aggttgtgaa atcagcgttt
     6241 tgacctgagt attagagaaa caaacggcaa aatagttcct catattttgc cgttttttga
     6301 gctaataccg aaggtcagct gattgaacac cgtttattca aaaaaaatgg actgcgacac
     6361 gacttttgtc acagtccctt cgtgtgtttt tctgccttct caaaaggatg acagtttctc
     6421 actcttttcc tatttcttct attcaatcaa ataactcatt ttatatacat ctaaaaattc
     6481 acctagatct gttcttgccc ctctaggcat gaccgcttcg atttggaagc ccatttttcg
     6541 gtaaaggtgt acggctcttt cattacgaac ttgaacgtca agctccaatc gaaaaagtac
     6601 acccatttcc ttcgcccaac tgatgatttc ttctagcata agagtgccta gtcccattcc
     6661 ccaatattcc tgcagaatac tgataccgac ttctcccacg tgagacaaac gaaattgact
     6721 gtctgccttg acagatgctg ttccaataat cgtcccttca tagattgcaa gcagcaataa
     6781 attattattg ctttcgtata tataatccaa ctcttcttct aaggtttctg gtggtaacag
     6841 gagttctgct tcatccaaaa ctaaaaattc tgtctcctta ttaacttgtc ccatcatcaa
     6901 aagaatgtct tttgcatctc ctggcactgc ttcccgcaag atgatcccaa cttcttcact
     6961 catgattttc agcccatttt tctagaattc gctgtttgaa ggtttcactt tgctccccgt
     7021 atgagattag tttcacatac cgcttttcta aatctgtatc acttttttct aaaatcagtt
     7081 ccaaataatc ctccggcagt gcttcttcta atagatttcc ccattcaaca acggaaagcc
     7141 catcaccttc aaaatattca tctaacccta aatctgctcc gctagctgct acccgataaa
     7201 tatccatatg atacaacgga atccgacctt gactatattc tctaataatc gtgtatgtcg
     7261 gacttttgat catttgcgtt atccctaatc cttgggcaat tcctttcgtc aacgttgttt
     7321 tacccgcacc tagatcacca gtcaatatca gattgtcacc tggcattgcg gaatgaccaa
     7381 ttgcctctgc gaattgttct gtcattgtta atcctgatag ttcgatcatt tttcatcttc
     7441 tcccgctctt ctttcttttt gccagtatat cgcgtaatga agttttttta aagaaaaaat
     7501 gaactagtca agaacagcag ctgctgtttt tggctagttc gcgtgtattt gttaatcaat
     7561 caatgtttgt gctgctgtga taatcgataa tttatataca tcttcttcat ttgctccacg
     7621 agacaaatca gaaaccggtt tgttcaagcc ttgtagaatt ggtccgattg cttcaaagtt
     7681 accgaaacgc tgagcaattt tatagccgat atttcctgac tgcaattctg gaaatacaaa
     7741 gactgttgct tgaccagcta cttctgaatc tggcgctttc agttgtgcaa ctgaaggaac
     7801 ataagaagca tcaaattgca attcgccgtc gatcatgtat tgtggcgcta gttttttagc
     7861 gattttagtt gcttcaacta ctttgtctac ttctggcgat ttcgctgagc cttttgtcga
     7921 gaagctcatc attgctactt ttggttcgat atcgaacaat tctgctgttt ttgcagaatc
     7981 tacggcgatt tcagccaatt cttgcgcagt tggattcacg ttgatcgcac aatccgaaaa
     8041 gacatatttt tcttgatctc ttccacgtac catgatcatc gctccgcttg tccgactgat
     8101 gccaggcttt gttttgatga tctgaagtgc tgggcgaaca gtgtcgccag tcgaatggat
     8161 cgctccagag accattccgt ctgcaatccc catatacgta agcatcgtac caaagtaatt
     8221 cacatctttt aaaatctttt ctgcttgttc tttggatgcc tttccatttc tacgttcaac
     8281 aaaagtatcc accatttcat cccacttatc gtaattatca gggctgatga tcgtaaagct
     8341 agatgttttg attcctctgg catgcgctgc atttcttatt tcttcttcgt tccctactaa
     8401 aatgggttcc acaagttctt ctgctttcaa ccgggcagct gcgcctaaaa tacgagcatc
     8461 agtagcttcc gggaaaacga ttttgatccc acggcgaaca attttaaatc ttaaactatc
     8521 aaacaattcc acaagatgac ctccagacgt ttttatatgt attatcatac actattccag
     8581 aaaaaaagaa cagtttattt tatctgtatc agtcaaaaat gctggaaaaa ttaagttttt
     8641 cacatacaaa gcgaactgca ctataacagg aaagcgctgt tttattacac tgtttcaggc
     8701 agttgccaat caatcggttc ttctcctaac gcaaggagcg catcattcgt tttcgagaag
     8761 ggtttggaac caaaaaagcc acgacttgca gataacggac tgggatgtgg agatgttatg
     8821 atgatatggc gactcttatc gatcattttg attttttctt gtgccggttt tccccacaag
     8881 ataaacacga ttggtttttc ccgttcattt aatttttcaa tgatcgtgtc tgttaatctt
     8941 tcccaacctt tcccgcgatg agaatacgct tgcccttccc gaacggtaag aacggtgttg
     9001 agtaaaagga ctccctgttt agcccatgac actagatttc catgttgaac aggtgcgatt
     9061 ccaagatcat cataaagctc tttatagata tttcttaacg atggcgggat ctttacgcca
     9121 ggttgcacag aaaaagataa gccatgagct tgattttctc catgataagg atcttgtcct
     9181 aaaatcacca ctttaacttc ttcataagga gtcaattcca atgcttcata gatatgatac
     9241 atatctggat ggatcttttg ggacgcatat tcttttttta aaaattctct tagatgcaga
     9301 taataatctt ttgaaaattc tgaggacaaa acttcttgcc aactgttatg aatgatttct
     9361 ttcaaaaact ccacctccta ataaaaagaa taactcacat tttcttcaat cacaagggac
     9421 aatcttcacc ctcagcactt gattaatatg gtaaactagg aacaagaagc agaaaggaac
     9481 gataatcatt atgatcaaat taattgcatc agatatggac ggcacattat tagatgccca
     9541 tatgagtgtt tctcctgaaa atgccgaggc gatacgtttt gcaaatgatg ccggtgttga
     9601 gttcatggtc gctactggtc gaaactatca agaagctcgc gcagctttag atgaggtagg
     9661 aatcgattgt gcgatgatca ctttgaacgg ggcacaggta tttgataaag aaggaaattc
     9721 attgtttacc gtgtcgattc caaataatca agcaatctct gttttagata ttctagatgc
     9781 taacggtatt tactatgaag tagcaaccaa cgatggtctt tattctgaaa atcaagcgaa
     9841 acgaattgaa agttttgctt ccatggtggc tacccattta cctcacctta cgtataaaat
     9901 ggcgattgcc atggcctcag ctaagctgga gttattgcac atcacatacg tggacagtat
     9961 tcgtgctatt ttggaagaaa agaatctgga agttttaaaa attatttgtt ttcatacaga
    10021 agggccagct gtattaggtc cagttggaaa gcagatttct cttttaggcg atctagccgt
    10081 tacttcctct ggtcaaaaca atatcgaagt gaaccataaa aacgctcaaa aaggaattgc
    10141 tgttgcccat gtcgctcacg agcgtgggat ttctttagaa gaagtcatga cgattggcga
    10201 taacttcaat gatgtcagca tgctgcaagt agcaggagtc agtttcgcaa tgggaaatgc
    10261 agaaatcgaa gtaaaagatt atgccaaata tacaacggac acaaaccttg aatctggcgt
    10321 aggaaaagcc atattgcgcg cgattgcaga agacttgtag ttttatctag aaaagaggga
    10381 tatagatgtc agaatttttc attcaggaaa agcagcttgg acgtgcaact cggacaattg
    10441 tcaaaaacga aaaaggacat tccctgtttt tgatggttgg tcgctgggga acaaagggcg
    10501 atgcattgtc tctttatgcc atgaatggcg atctcgttgc tcacatcaag cagataagcc
    10561 tgacattcgg tacgcgtttt gagctgtata aagagttcga aaaggtggga accatgcaga
    10621 aaatattcaa ttggcccggt gacttttatt atattcgtag acttcactgg acagtccaag
    10681 gtgatatcta taatcatcaa tatcaaatcc atcatttcaa tcagcaaatc atgaaaatgg
    10741 ataaagcaac acttttttca ggagattact atgtattgga tgtctcaaat gatgaagatg
    10801 ctccagtttg tatttgtatt gctgcggttt tagattactg gctctaccgt aaagacaaaa
    10861 gtcgcgataa gcctgtttct taccgactta acttaagtta aaaagatgta cctgaaatgc
    10921 ggaaatacgc atttcaggta catcttttta gatattcttt tgcatatgat cataaagatg
    10981 accactgatc atccgctgac cgacaacttt gaagtcatgg cgttcgtata attttttagc
    11041 ctttggattt ttttcatcca cacttagacc gatgacggag cgattagctt tcttcgccaa
    11101 tttaggtaat gcttctaata atcgcgaacc gatgccttgt cctctaaaat cttctgatac
    11161 actgatagag tccaaatacc attcgttggg aaacgtttct ttatcaatga acaaacgtgt
    11221 agacgcatct agctgatatt tctctaagat aggtaccagt ggctgatcga tgattgattc
    11281 ttcttcatct gtataaccgt aagcaactcc tgcaacttta ccgtctattt cttctacgat
    11341 acctcgtgaa taactgtaac gataatttgg atcagtgatt gcttcttcta aaatctctaa
    11401 agtcttttgt tcccctgtct gcttcaaaaa tggcagttcc atatctttta gaatcactaa
    11461 gatcaatggg gcgatttctg gagcatcttc ttttctcgca aaacgaatca tagtttcctc
    11521 tccttttctc cattctaaca tgcagaaaaa gaaatcgcaa aatcgttatt ttaaaaaatc
    11581 ttcaatcttt tcagtaatga aataaccatg agtaacgatt cttgccgctt cagttcggca
    11641 ataagggcag acgactggca tttctttatc attatgttcg actacattat tgacttcttc
    11701 gtatgcaaat ttttttccgc aattagggca tttcattttt tccatgttat ctcaacttcc
    11761 tttcatcttt actctcttag cttactctta tattgtaaaa aagagaagca atatccttct
    11821 tctatgcaga tatccatacc tgacggtcct gttctccatc tattaatcgg actttataat
    11881 aaggacgttc atctttgact agaagtgtcc actctttcac ttttgctttt ttcccgtagt
    11941 agctgactgc ttccttcatc gctgcttgcg gttgtttgaa ctcttccgtt ttttctatat
    12001 caaaaagatg ttctgcggcc ttttccgtgt catttcgtac ggttgttttc ccagtggatg
    12061 gattgactgc tatgacttgt ccttcacaaa aaaactggta ttcatatgtt gcattcgtag
    12121 gattctccaa atctttttca actgcaaaat gaatagcaga tatgttatgt tcttttgttt
    12181 cgttttttac gattgctgct gcttttttca atgagatccg atattcctgg tcactaatat
    12241 catccatgtg aaaagcggca ttgagcagtc ccgtcatacc ggtaatccct attacgatga
    12301 gggcagcaac accaaacatc atttttttca ttcttcattc tcctgtttct tttttttaaa
    12361 gaatatcaag aaatgaacga atgaaaaatc gaatgaaacg aaaaaaaagt gacaattttg
    12421 ttatctttat tcgtgcagtt ctaatggcaa tccgtctgga tcaaagaaaa acgtcatttt
    12481 ttttcctgta aaggtatctt ctctaatcgg ttctgattcg atccctaagg actgcaattc
    12541 tgcaattgtt tcttcaatat cgtctacttt gaaagcaaga tgacgcaagc cacaagcttc
    12601 tggatagttt ggtcgttgtg gcgcattagc aattccaaaa atctccaatt cagcacttcc
    12661 tagtttcaga tctaatttat agtcatctcg ttctttgcgg taattttctc tgattacttc
    12721 aaatcctaat ttattaacat aaaaatcacg tgatttctga taatctgaaa caatgatggc
    12781 tatatgatgg atcgttgtaa ggttcatatg agcactccta ttcttcgttt aagctcggat
    12841 ctatattaat ctccagttcc attgtaaatg tgtcagattg aaaaaaatag ctgtttttcc
    12901 tttttataag aaaaaaggca aaaaaaacag aaagtaggtc ctccaagaca ctcttcctgt
    12961 tcccaatata ataaatgaaa gtaagaaatt atattttcaa gaaaacaaac agaaaattat
    13021 cataacacaa attatttata attgaaagac tagttttttt tcaagaaaag catacaatag
    13081 attgtgttag taaatacaga acacactaca tttagtgtct taaggagtcc tcatacatga
    13141 agtcatattt tcagttttta gctcatcagc taaaaggctg gccgcaacaa aattattatc
    13201 ttttcttttt cagtcttggc tgccaggtca tgactcttgt caatagtccc atcacttggc
    13261 tgtccgttat tacatttatc ggtaccacgc ttggtgtatt gtgtatttta tccatcaatg
    13321 cggcaaaatc agtcaacggt attttaggta ttttgtctgc gatttgtttt attatcgttg
    13381 ggttttcagc aaagaactat ttaagtatcg gtgaacagat tgcttatatg atcacattgg
    13441 atatccccgt attgttaagt aaagaatgga atcataatat ggcttcaaaa atccgaaaat
    13501 tcactggaaa aagctggttt atcgcaatca tcagtacttt ccttgtctat cttgtttcag
    13561 gattcctgat tggtcgttat acagatgatc ctcggccgtg ggtagatgcc attgcttttt
    13621 ctattagctt gactgctggt atcatcagtt tcttgcgtta caacaaccaa tattactggt
    13681 ggttagcttc agggcttgct caaatggtcc tttggtatat ttcatttacg catggatctg
    13741 caagtttggc gatgttcgtg aacagctctg tttacttgct gaatgatgtc cttgcattta
    13801 ccgtttcacc ttggtataac caaaaagaac gtgaacgtat gcaaaaaatg gaagaagaat
    13861 actacaacgc acaacaacac gcataaaaaa actgagcgtg ggacaaaagt aaagaacact
    13921 tttgtctcac gctctaaaca cgtataaacg gcggaatcag aagcaactcc ttcggaaata
    13981 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
    14041 ttaaacgctt ctgtcccagc ctctattttt tatagtaatt ccttcagata ctttcttgcc
    14101 tcatcattga aacgcagtga ttcttctaac ttctgataat attccaacgc ttgtttctga
    14161 gcttcagtaa aacgataatg ttttcctttc catttgtcga ttgtctcttg cacatttcca
    14221 gattggatat ctttcactac tagttgccat ttttcttttg agactgtacg gtctttctta
    14281 tcagcagatt gtatagcttc tcgttcttta cgcaatactt ttaaaaaacc atttctgact
    14341 atccgaggaa tgctgtcgaa gaacccttct gtaaaaccat agcgacaata ttgcttccaa
    14401 ataaatagat agatttttgg ttggatgaat cgttggtttt tcgcaaaata ttccatctga
    14461 gcagtgactt ctggtgttcg ttgacgtttt gtctttctca acacggaaat aaatgtttca
    14521 aaataagcat taggatgtac tggcacttct tcatatacga tggattgcca atctttacgt
    14581 aaaaatgcgc gatagttgat caagaattct tctacatcct ttactaattc ttctgcaaag
    14641 tcgtcaaata gttctttttt tatctgtcgc ttttttgact gcccattgat gtgttcactt
    14701 attttcttga tttcattttc caatgcttga taatccgcta tgtaaatcgg catttctact
    14761 tggcgaaatt ctgcgagccg ctgagctaat tttatatccg gtaaatatgg ctgtttcatc
    14821 cgacgatttt ggtacaagac aaaacagtat ttttgccata gtccttcagg aaaatcgata
    14881 ttcttctgct taagccataa taattcgtct aaggccagat caacttttgt cattccctga
    14941 tggatcgaag ctgccactgt cggagagaca tgcaggtgat cggcaaagtg gttgatgcct
    15001 agttttagga tctttccggt ttttggcgat ttaacgatgt attgcacttt taattctcta
    15061 ccacactcac aatacaattt ttgctggctt cgttttgttc gaaaaaacgg attgatcttg
    15121 aagtcagaaa acacccaatc tgttgcagct aaataatgtt cttccataaa acgagagctg
    15181 attagatatc ggcggtgctt atcgactagt tcccgctgtt ttttcgttaa ttttgcatag
    15241 atctcccaac gttgactgct tgtgtattgt ttagaaattt tgatgggata gcttttgctg
    15301 atttcttttg gaaaccattc ttcaaagacg gattgccatt cttccacggc tgtttctttc
    15361 ctttctaatt acaaaaagct ggctactaac ataacgttag caaccagcca gtgtttcttg
    15421 ctaaattagt gagttgttcc ggcatttccg gaaatatctt ctttattttc tactgctgct
    15481 tcaactgcat aagtcaaaga agaagcaatg tcttctaaag acatagaagg cgtacccact
    15541 ggacgatcga tcacttgatc gggtgagaat ggcacgtgga tgaacccacc tcgaattgcc
    15601 gggaattgtg tttcgatcaa atgaagcaaa cgatacatga tatcattgca gacaaatgtt
    15661 cctgctgtat aagagatgta cgctggtaga ccatgatctt gtacattttt tgtcattgct
    15721 ttgattggca atgaggtaaa gtaagcggct ggaccgtttt cttctaaagc cgtatcgaat
    15781 ggctgatttc cttcattatc agggattctt gcttctgcta gattgattgc gacacgttcg
    15841 aacgagactg ctgaccgccc acctgcttgt ccgacacaga tcacgacatc tggttggtgc
    15901 tgttggatgg cttctttgac tacttgactg cttttttcga atacggtcgg tacttccact
    15961 ttgatgattt cagcaccgct gatttcatct ggcatttttt ttactgcttc ataggctgga
    16021 ttcactttgt ccccaccaaa tggatcaaaa cctgttacta gtactttcat ctgttgttcc
    16081 tccgtccgat tgatttaaca tatggttatc ttttatcata ccattttcct tgaacagcgt
    16141 aaataaagga aacaatagtt taaataacat aacagatcaa acttaatata ttcttataaa
    16201 aaaatagtgt tccactacaa tgttagaaga ggttgcgaca attattctgt cacaacccct
    16261 ctcttctatt atttaaatag aatcatataa gcaatttgga aaactagcat aaataacgca
    16321 ataaacaact ggtttttgat cactccgtat ttgtctttca tctccagcat cgctacaggc
    16381 acgatattga agttagcagc catcggtgtc agcagggtcc cacaaaaacc gcaagtcaat
    16441 gcaaccattc ctattaacgc tggattcgct ccatgactaa atacgaatgg tgcaccgatc
    16501 ccaaccgtca tcacggtgat ggccgcaaat gcattcccca tgattaccgt aaatatcacc
    16561 atgccaagag cgtaaatgat gatgccgatg ttcacatttc cttctggaac gatcgtacct
    16621 actgcattgg aaatcactgt cccaactcca gcgcttgtaa ataccgcacc tagagaagct
    16681 aaaagcattg gcaacatgct taacggccct actgtgccca gcatatctgc tgcatcatct
    16741 aaaaatgtac ttggtcgatt atctcgagaa aagaacatca agattagcat ggctgcaaaa
    16801 acaccgacgc ccacgccgac taaagcacct aaattggtaa atagagcaaa caagatcgca
    16861 aagacaccga tactcaaagc tggaataaaa attttcattc ctagtttgtc tgacattttt
    16921 tccatatatt cttttgatgg aagccggctt tctcctttgc tgactttctt agcaattgct
    16981 ggtatggtca tcgcaaagat caagccaccg ttcacgattg ccggaagcca tcttcctcct
    17041 gcaataacta cccctaagac cgtccagaaa aaggctgacc cataacgatg aggattggtt
    17101 ttatctgtta aatttcttac tccagtgtaa atcaatacga gtcccatcac gatataaagg
    17161 atctccagta atttatcgcc taataaaata tcaggatttg taaaaaagtc catcttattg
    17221 cccctttcct ggataatatt tcttcgctaa tttcttttct ttaagataga agtagacact
    17281 tgccacaact aaagccgtga tagccactgg aacttccact aaagcaagct gtcccaatgt
    17341 tacgtcatat cctagatctt tcagtgttga ttggactagc aacgcccctg ctcctccgat
    17401 gaatagtact tggccaaaga accagcagac attttccata gctgaagcca taccttttag
    17461 ttcttcttca tgcttaggat ggacttctaa cccttttgat tcgatggtcc ccaatgccat
    17521 cggtaaaatg attggacgaa caaatccagc aactccacca aaactcacgt taaaagccgc
    17581 aaagatcatt cgaaagatcc cataaatatc tacaatcata ccagcagata ctttcttgaa
    17641 tcgcttgatc aaggaagccg ctgattcttt cagaccattt ctttcaagcg ttcctgttgc
    17701 caacataatg atgataaaaa tcgccatccc gcgattgttt acaaaacttg tacctaaaag
    17761 ttctaacatt ccttgaatgc caagaccgcc agtcaaagct gtaacaacta aagcaaccat
    17821 tacgatcaat atcgagtcta atttaaatgc aaacccgaga ataacaatca agacgcctaa
    17881 aaggctaaga taattttcca tatattttcc tccataaaat gacaatattt ttttaattat
    17941 aaagcaaaac acataaaaaa tatagttgtt ttctcacgaa atagacacta tttttaaata
    18001 tttttcgtta tttttttatc acaaaaatta tcttacttat tgtaaggtaa agcttttttt
    18061 aatttattgc ccgataatat atagaaataa ggtctagtta ctgttttttt taaatgatta
    18121 ataaataaca acttctttct atgaaattgc aaagcattca tcttcttttc cgctaacgat
    18181 atatccgtca gtgcttggta cactttacta aaaagtatga gattgccgat atctggatag
    18241 gtgtgctata ctacagaaaa agatatacaa tctgccaccg ggtaggataa agaggatgat
    18301 cattccatta ggtctttgaa gggatgattt tcctcttttt gtgtattttt ttatagttaa
    18361 ggaggaatgt aagtatggca tggatggaat taatcatagc aggaattttg gaagtattct
    18421 ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat caattattct ttttacacag
    18481 tgattggcat gattgctagt ttttactttt tgtcaaaggc aatcaaaagt ttgccgatga
    18541 gtctctctta tcctatctgg acagggattg gcgcagttgg ttcggtcata attggtgtgg
    18601 tcttgtttca tgataagctg aatctttcca cttggttctt tgtggggatg ctactcatca
    18661 gtatcattgg catcaagatt actagcggcc actagacact tggaataagt actgatcaga
    18721 aaaaataagc ctttttattt aatattcaat ttttttatga aaaaataaga aataagtcca
    18781 atgacctagc gacttctcca ctcatcaggt gtatgcttgt actatctata gaaaaggagt
    18841 gaatattttt gaaaagtctt tcggtccaag aattatccaa gaactttggt cacaaacaag
    18901 ccctcagcga tgtcagtttt agcttatccc ctggcaaaat cgttggatta gtaggtccta
    18961 atggcgctgg aaaaacaacg atcatgaaag ctattttagg actgatccat tactcagatg
    19021 gaacaatctg tattggtgaa aatgagattt ctccctctag tcataaaggg ttggaacaag
    19081 taggagcatt gatcgaatac ccgggtatct atccattctt aacaggctat gatcatctga
    19141 agttattctc tgaaaccaac gatgtatctg ctatcgacac tatcgtgaat cagctcaaga
    19201 tggaaaagta cattcacaaa aaagcgaaat cctactcgtt gggaatgaaa caaaaattgg
    19261 gaattgctct tgccttactg aaccatccgt ctatcgttat tttagatgag ccgatgaacg
    19321 gtttggatcc acaagcaaca agagatgtgc gaaaaatcat taccgacttg gctactcagg
    19381 gcacctcttt cttgatctcc agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc
    19441 tgatcctgat caatcaagga aagatcatca aacaatgtac aatggctgaa ttgaaccaaa
    19501 cagcgcaaga tttcatcgtt atctctacaa caaatgatct agctgctcga acgatcttga
    19561 cagaagctgg atatactttg gaagatcctg ataaaatcaa gcttttaaaa gaaaacgaag
    19621 aattattagg aaccgtgatc cagctattgt ctgcacaaaa tatccatgtg acagacgtcc
    19681 agcatctgac gaatgatttg gaatcttctc tattggaaat tctggattca gtaaaggggg
    19741 aagaagaaaa atgacattgc ttgtaaaaca agaattattt aaattgatca agaaaaagtc
    19801 aactgctgtt ttatctgttc tattagttgt tttgctcata ggaacagcac ttctagccaa
    19861 aaaatacacg acgatcattg atccagttga aatgactgct cagcttttta gtgccacatc
    19921 ttggattgtc tttatcatga ttgcagctgc aagcacgatc atttcgatgg aagcacaata
    19981 tggtacattg aaaaatctcc tttatcgaaa gtattctcga ggagaaatat tagtaagcaa
    20041 atggatcaca ttagttattt attctgttta tttatacctt ttagctatta ttgtcactgt
    20101 gttgatgaaa ctgattcttt tcccttcgat ttccttcacg gagaaggttt ctactggtca
    20161 aacactgatc caaagcttgt tcacttatac tttgggtagt tatatcggtt tatggctgat
    20221 tttaagttta gtgttgatga ttgcttgctt tatcaatagt tccggcgcat ccatctcagc
    20281 aggaatcgtc ttttattttg cttcgtcaat catatctggt atccttattg cactcatcca
    20341 aaaatgggag tggatcaaat ggaatccgat cagtatgttg aatctgcaaa accaaattgg
    20401 aaacgaagag atcatgaagc aattgactca tctttctact aatcaaatgc tattcggtaa
    20461 tttagcttac atcgttttgt tcttagcatt aggatatctc gtatttaagc gaaaaaatgt
    20521 atgatgactc atcaaaaaag aggctgtgaa agagacgttc agccttattg ccgattggct
    20581 gtctcttaaa caccgtttat tctgaaaaag gagctgtgac aacatttgtg tcacagctcc
    20641 tttcctagta tgtttttgaa taaacgacag gctcacaacc tctctttata cttcttttat
    20701 acttctatac gtgaagcatc ccagtggcaa ataaaaccat tgcaatcact ccgaccatcg
    20761 tcacacctat aagtaaaatc ttatctaagt ggatatgttg tacaggctcg ttttgctcct
    20821 tacgtgcttt gaggtaaaaa taaatgcctg gcaagaaaag aacaagaaca agcattactt
    20881 cattaaaggc tacgactagc atagcccata cttgaaaggc acttgctaaa agccctacta
    20941 aaaattggac ccattctttc cgctgataag aaagtttcat ttggtacaat cctaagaaaa
    21001 gccacgtgaa gaaaatagct gctgtaccaa gtgtgtaagc aaagttataa gcactgtcag
    21061 tgaataataa tgaaagtaaa aagacattcg tacataacgt cgtcaatacg atagcataag
    21121 ttggtgcccc atttttgttg agttttcccc atgctttagg caaaagtccg tcttcagcca
    21181 tgtttcccat tgtttcaatc ggtaagatcg tccatgagag ccaaacacca actaaagaaa
    21241 tgatcaaacc gccgttgatc acgactgctc cccatttacc aaccatcatt tcaaacacat
    21301 agcccatagc aggctgcgcc ttgatcgttt gcagctgatt catcgtcatt gtcccatatg
    21361 gaagcatgga aaccaagacg taaataataa tcaagccgaa tatccctaga atcgttgctt
    21421 tcccaacatc tgattgtttt tttgctcgtg aacctacgac agaggctcct tcaataccaa
    21481 caaataccca aagcatcacc atgatgcaac tcaatacttg actggaaaac gggatcggct
    21541 gtccattcgc attcaatata atgtcgctag aaaagttatc aatgaattgt tggatcgaaa
    21601 acgtgctgat agctactaca ataaataaaa ataatggcac taatttacag aatgtgacga
    21661 tagcattgac gaaactagca ttttcgactc cccgattcac tagccatgca tatccccata
    21721 aaaagactgt actcatcacg atcgatgcca gattttgccc accttcaaat actgggaaga
    21781 aatatcctaa tgcgctcatc aacaatgttg caaaagctac gttgcctaac cagcatgtga
    21841 gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac
    21901 taaagatccc gccttttaaa tctggacgtt ttttattgat attattgatc gacaaggaga
    21961 gcatcaacat tgctgttcct acgagtaccc aactgaaaat agctgggccg ccagccgatc
    22021 cggagatgtc cgtcataata ccaaatacgc cacctccaat cgctgaacca attactaagg
    22081 cagcaagtgg aattaaacca attccttttt tctgttgctc tgtatccatg taagcatcct
    22141 ccaaaataaa accgctttta tcaaaaagta taacaaacct tttttatttt tacttaatta
    22201 ataaaaactt taatagaaat ataattctac cggctttttt ctcattatac aagcaaagcg
    22261 cctagtccct ttccaaatta caccaccaaa aaacttaaaa aagatgctat actaaaagga
    22321 aagaattgga ggatgatcca tgaacatttt atatatatcg atttcaggaa atacgcgtgc
    22381 ctttgccaaa catctagcag aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc
    22441 tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt
    22501 ttttactttc gttcctactt atttagacgg cggaaatggt ttagataacg gtgatacaga
    22561 aatcttgacc gaaacgatgc gtgaatatct ggaacatgaa gacaatcata aactttgtct
    22621 tggtgtagtc ggtagcggaa ataaaaactt caataatcag tattgtctaa cagccaaaca
    22681 atatgcacaa acatttggct ttcctttctt ggcagattat gaattacggg gaacaccatc
    22741 tgatgtgaca cgaatttatc agattttagc agaaaacaat aaataataaa aaagaagaag
    22801 ctgaaaaata gatttcagtt tcttctttcg tttctattgt ttctatcata ttaagctttg
    22861 acatccatca caggtgcttt tgcttttgct ttacctgttt tttccaagac aaagtttttt
    22921 acacgatcca tattggttac caagacgatc atggatgttt ctttgcctgc tgctttgatc
    22981 gcttctaagt cagcttctgc tacaagcgta tcaggtgtca cttgctgccc ttctgttact
    23041 ttcacatcaa atggcgtacc gcctaagtca actgtgttga ttcccatatg aagcaagatt
    23101 tctaaaccat ttggcgttgt gatcccaata gcgtgtttcg ttgggaagac agagctgact
    23161 accccagtaa ctggagaata gatatgtccg ttttctggtt ctacaaagta gccttcaccc
    23221 atcatctttt gtgcaaaaac tggatcactt gctttatcta aagaaacaac tgttccgtca
    23281 gcaactgatg agaggatagt cacactttca gctgaagttt gaggtactac tttttcaggc
    23341 acttcattta ctgtggctgt ttcagtcgct aattcttctt tttctaaacg gtctttagga
    23401 ataccgaaga agaatgtagc aacgaagcca ccagcataag ctgccaataa tccaaggatg
    23461 tagcctaacc atttaccgtc agagatcaat gggatcaaag cggctcctga aggtccaata
    23521 gcgatagccc cgacatttcc gatcattccg atcactgccc cgccgatacc tccaccgata
    23581 caagcagtaa taaatgggcg tcctagaggt aatgttactc cgtaaatcaa tggttcgccg
    23641 attcctagga tcccgactgg taaagctcct ttgatcattt ctgttaattc tttatctttt
    23701 ttgcagcgaa tccataatgc caatgctgca cccacttgtc ctgcaccagc catcgcaaga
    23761 attggaagca atagcgtacg accagtttca tcaatcattt gcacatggat tggagtcaaa
    23821 atttgatgca gtccgaacat aaccatcggc aagaataatg tgcctagaac gaagccggca
    23881 aatgctccac caacgccaag tacccagttg atcccaccga ccatgccatc agaaatgaaa
    23941 cctgctaaag gcatgatgaa gaaaatttca atcagaccga tggcaatcaa tgaaagagtt
    24001 ggtgtaacga tgatatctac cgcatcaggt actactttat gcagtttttt ctcaacgatt
    24061 gacaatagcc aaacagcgaa gatgacacct aggatcccac cttgtccagc tgcaagagct
    24121 tcaccagtaa ataagttttt cagtggtgct tccggattca tccctgtcag catcacaacg
    24181 gccccgataa ctccaccaag agttggtgtt gcaccaaaaa cttgtgcagc attgatacct
    24241 gtatagataa ctaggtagct aaacatcccg tttttcaaga tgttcatcac atcgatatat
    24301 tgttgccatg ttgctgcatc aagtgttccg gctgtgacta agtttgctaa aactgcggca
    24361 atccctgcca cgatcccagt accgacaaat gcaggaatca ttggcacaaa gatattagaa
    24421 atgtctttta ataccgattt gattttagat ggtttttgtt tcgatttttg tgccgctttc
    24481 atttcttgtg cccgttcgtt tacacggtct tttccagatg ctgatgaatt tgctgtagct
    24541 gtacttacag aagaaagttt ttctcctaat ttcacgcctg ccaaatcaac catttgctgt
    24601 gctactttgt tcactttccc aggtccgatg atgacttgaa gctgttcatc ttctactaca
    24661 ccaagcactc ctggaatcgc ttttaattca gagacattta caagatcatc attgatgatc
    24721 ttcatacgca cacgagtcat gcagtggaca atgctttcta cattgcccat gccgcctgcc
    24781 cctttataaa tttgttttgc cagttcttcc aaagtcagtt cgttttttgc catggtcttt
    24841 tccccctcat aaggtccctt ttacaaatcc gtttgcttca gctaattttg cttgagcttc
    24901 ctctttatca ctatttgtca aaatcatgac gatagccagc ttaacatttt gatctgcttc
    24961 ttcaaagtac ttttcagctg tttcgtaatc agcttctgtc gcttgcatga tgattcgttt
    25021 tgagcgttcg attagttttt catttgttgg tttgacatcg accatcaagt tattgaatac
    25081 tttacctata ccaatcatac tgatagtaga aatcatattc aagataagtt tttgtgctgt
    25141 tcctgacttc aatcgagtag agcctgtcaa aaattctgga cctgcatcga cttcgattgg
    25201 gaaatccgcg tgtttactga tttcggcttg tttattgcaa gaaatcgttc ctgtgacagc
    25261 accgatcgat tttgcgtaat ctaatccacc gatcacatat ggtgtacgac cgctagctgc
    25321 gataccaata actatatcat tttttgtcaa attcagcttg attagatctt cttgccctag
    25381 ctcataagaa tcttcggccc cttcgacagc tacagtcatt gcttcttctc caccagcaat
    25441 tagacctatg accatttccg gttctacacc gaatgtcggt acgcattcag cagcatctaa
    25501 cacacctagt cttccacttg ttccggctcc catataaatc agacgtccac cttttttaaa
    25561 ggactcaatc gtgttagcaa tcactggttc gatacttgtc agttcttttt caaccgcagc
    25621 ggccactttc tgatcttctt tgttcattag ttgaacggct tgagaaatac tcatctcatc
    25681 taatccaaaa gtttcttggt ttctgcgttc cgttgttagg ttctctaaat tgattttgct
    25741 catttttatc ttccttcttt tcttataaat tgatagttca taccagcttt gatttggttg
    25801 atcaatggta gctcttcttt gataacttta gctactacat tcactttttc atccgctggc
    25861 aaatcatatt tggttaattg gatctcaccc atatagcgca aatacttttc gttatctaaa
    25921 gtaatagacc ctttcatacg cgtagctgta tttcttgctg ggataggcgg aatctctcga
    25981 aaacgtgcat ctacgcttcg tatgacatct cttgcatcgt cttgccgatt cgtatgcttc
    26041 cctaatatat attcgtaaaa ttcttcatct acaggttccg tatggagcaa gactgtctgt
    26101 tctttttgga aagaagcaaa ttgctcctga acgttttctg acagacctgc atctccgatg
    26161 taaacaatat ccgtgttact tgctaataga tctagagcgc ctgcaagagg atggatccct
    26221 cgatgttctt ccaaagttgg caatccttga tatagtgggc ctctcaactc tgtatctcct
    26281 ggtacaaaac cttggacaag gagaccttga attttgagcc attgattttt tttctgatac
    26341 cattctttgt ccagccctgt ttcgggacgt ggataataat tatgccaagc ttcaatctgt
    26401 gagaaatcag cttctgcttc tttcaattca tcgatatctt taggcgtgat cgtgctagca
    26461 ttcaaactga ttttcaattg atgtgaccag tcggcaatct gctgattgga aatatgataa
    26521 tccattctta aacctgttac accaattgct ttcaaaggcg ttagatcttc taatgaaaaa
    26581 cccgctcgtt ccaatgcctc tcctgaaata tcgaccatca attctagttt attctttttc
    26641 gcttcgcttc ctagttcgat caatcgcttt tgatatgccg aaacatcatc ttctggtata
    26701 tgcatggaag tgaaaatacc gacaaagcca ttggatgcca tttggcggat atactgtttc
    26761 ttctcttctg acaaagaatc gttcataaat attgaaaagc caaacatatt atccctcttt
    26821 ttctgtatcc gtcttcattt ctatgactag agtatatatc taaagaaaca aaatttcaat
    26881 acttttatta aaataattaa atttcatttc taaaggttga gtttatatta tcaaaagaat
    26941 tgtcataacc taaaaaaggc actgctaagc aaaagcagtg ccttgaaata ttttatttaa
    27001 ataacacaaa aacataatta ctcataagaa accaattctg tattatctac catgaatgta
    27061 aagaacccct cattaggcat cggatgaaaa taagcattga tcactcttat caaggcatta
    27121 tggcaaacta acagatagac attttcttcg tcttcgatgc attctttcaa taaaggaacg
    27181 atccgtgcat acacatctaa gactgactcc ccatttggga atcgtacagc gaacgctaaa
    27241 cgtgcttttt gaaagttctc atctttgctt ggcagtccat cataatcccc aaaatccatt
    27301 tctaccaatc gttcatcaac ggtcagcggc aagctgagac gatcagcaac tgcttgtgct
    27361 gtatctcttg ctcgttgaag aggggaatgg atgattttcg tgattggaac aggcaaatct
    27421 actacctttt ctgctagttt ctgtgcctgc atctgtcctt tttcagtcag cggcacatct
    27481 gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt ctccgtgtct cgttacataa
    27541 agcatgtgat tctctccttc gttggcaaag ttccccttaa ccataccttg ttgttcttga
    27601 gattacaagg gttagattct ctcaagagaa ataatctcag tagatacttt atccaggaag
    27661 gtttgatcat gttccactag taacatggca ggtttcacgt tcaagatcag ttgttctaat
    27721 tgttcttgat tgaagacatc caaataattc aatggttcat cccatgtata tagttcagct
    27781 ggctgtgata aagatttagc caattccact tttttccgtt ggcccatact catctgctcg
    27841 atcttgttat gaaaaacatc tctttccatc ccaagctttc ggaggttgtt caaaaatgct
    27901 tggtagtcta cttggttttt ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa
    27961 ttttggcttg cataactaat gctcagatgt tttggctggt atttttctcc tataaccttg
    28021 ccgttgaatg cccccagaag ataatggata atggatgact ttcctgcacc gttaggacct
    28081 gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa ttggctcgaa taacagattt
    28141 tcatacccta attgaagatc ttctacgctt aaaagtcgct tatggtgaga cgcttggcta
    28201 ttcatcgtca aagaatcgat atattcgata tcttttaata gtttttcttt ctcgctgatc
    28261 tgggtctcca tccgattcac gattgctttg gatcgtttca tcgtccgtgc agcatcagca
    28321 cccactgctc ctttattcac tcgtctagat tcagtatcga tgaatccgac ttgtttcttt
    28381 gttttatctc cttctcggga acgagaccat tcggcttttt cagctgctgt tttctttagc
    28441 ctactgactt cttttttcaa tttttcattt tgagccattt caaattcatc acgaagtttt
    28501 ttctgttctt catagataga gaaattccct tgataaagtt ccagttgact tttttcgatt
    28561 gctaaaacat ggtccactac ttcatcgata aatccccggt catggctgac cacgatgaag
    28621 ccttgttttt tctttttcaa ataagccgct acttgttttc taccagagat atccaaatga
    28681 ttcgttggtt catcgattaa cgggaaatga gtgtcatcca caaataaaag tgccagtagg
    28741 acttttgtct tctctccccc cgatagtgtg ctgaattctc tccataagat ttcaggatct
    28801 gtttgcatca attggagctc tctttcgatt tcccatatct caaaatccgt aatatcattt
    28861 aacacgtaat aggttaaacg ttctttatct tttgtctgtt gcgggaaata ggcaaattct
    28921 tgctgatgga ttacttgccc ttgataaggt agtttgtttt gtagaatatt cagtaaagtt
    28981 gtctttcctc gaccgtttcg tccgataagt cctagtttcc attgtgtgtc aaaatttaga
    29041 tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt
    29101 tcgattttag acattttcca tcacctcgta aaaaaagacc agtcctattt tcagaactgg
    29161 ctaaagagga tgcaagggtt ggatacacac aaaaagaccc atacatcgaa aaaaccaagc
    29221 caatcccgaa aatctgcaca ctgcttcctt gtctccacaa ggtacagtct gaatgcagct
    29281 ttcatcaatc gacttaattt ttcaatgcac ggatccctca ctttgtccta tatttgaaat
    29341 tacgatagca taaagaaaat gattttgtca actacttcgg gaggatggtg aaaaatctgc
    29401 taagtgtctg tcctaagatt tttgtttttt ctaatgtttg cctgtgttcc attgcttctt
    29461 ctaaacctat tggacccaaa tgaatactta agacaattgg gagaaaatca gaaagtcctt
    29521 ctagctcttg gtctctactt ccgcaaatag cgattattgg ttttcgatat ttttttgcaa
    29581 gcactgctac accgtatgga acttttccgt ggatcgtttg ctgatcgatc cgcccttctc
    29641 ctgtaatgat gacatctgct tgttcgatct ttttttccaa tccagtgatt tggctgacta
    29701 aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag ggctccacca ataccgcctg
    29761 ctgctccact tcctggaata tcatctaaat ccacctccca gaaatctttt atttgctgag
    29821 caatctttcc tgcttgttta tctaagaaag ctagcgtcgt atctattcct cctttttgtc
    29881 ttccaaattg ccaagcagct ccttgttgac ctgtatatgg attggtaaca tctgcagcga
    29941 taatcaattt tactttagaa acaagaacgt ttcttcgatc aaaatgaagt tttttagtag
    30001 ataataaagg atttccttct gtgtaccctt ctaaagaagc acccaaggat tggagtaatc
    30061 ctagaccacc atcgctacac ccgcttcctc ctaaagtgac aataatttgt tctacttctt
    30121 gaagcagtgc atctttaata agttctccta aggcataact ggaagcaagt cggatagttt
    30181 gatccgttgg tttatcaagt agatctaacc ccaatgcttg tgcagattcg atgactgcgg
    30241 tgttttttcc ttgccaagtt gtcaataaat agctgatttt tcgctgacgg aataagagat
    30301 ccatcccatc aatggtacgc catgttcccc tgagcgcgta atagattgct tctgcagtat
    30361 tttctccgcc atctgcaatc ggcacattga tttttcttcc tttacgatct tcccagccat
    30421 ttagaactgc ctgatttagt tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg
    30481 ccaaaatatc catctgtatc cctcactttc ttcttaagta agacactttt tttcattttt
    30541 ttcaaacaaa acaatgctat tttcttccat ttttctatat actaaaaggg aactcattga
    30601 ataaaggaga gaaaaaacat gaaaatcgca atagctgggg ccggagcaat gggcagccga
    30661 atcggattga tgctacatca aagcggaaat gaggtcctct tgatcgatcg ttggcctgct
    30721 cacattgaag ccattcgtac aaatgggttg attgctgact ttaacggtaa agaagtggtc
    30781 gcaaaactgc caatctattc accagaagaa atcatcgaaa gcaacgaaca cgtggacttg
    30841 atcgtagcat taacaaaagc caatcaatta gatgatatgt tttgttcgat ccaatccatt
    30901 attactgaca atacttatgt tttgtgttta ctaaatggat taggtcatga agatgtactg
    30961 gaaaaatatg tgccaaagaa aaatatctta tttgggatca cgatgtggac agctggtcta
    31021 gctggtcccg gaaaagtcac attgttaggt gatggagaaa tcgaactaga gaacttggaa
    31081 ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct ttcaagaagc aaatttaaat
    31141 ccgatctata gccataacgt tcgttattcc atttggcgaa aagcttgtgt aaatggaaca
    31201 ttgaatggtt tatgcacgat tttggattgt aatattgctg aactaggcgc gcaaaaagcg
    31261 gcagagagca tggttcgaac aatcgtatct gaatttgctt ctatcgcagc aaaagaagga
    31321 atcatactag atcaagaaga agtgtaccaa catattgctt ctacttatga tcctgacaac
    31381 atcgggttgc attacccatc tatgtaccaa gacctgatca aaaaccatcg tttgactgaa
    31441 atcgactaca tcaatggtgc catttggcga aaaggacaaa aatatgatat tgctacacca
    31501 tattgtgcct ttttgaccca attagtccat gcaaaagaag gaatccttgg cgcagaataa
    31561 aaggctctct gatgagaatc agctgaatca gcagctggtt ctcttttttt aagcccaata
    31621 aaagatcaaa aagaaaacac aactctcatg atgccataag aattgtgctt gtttgtttca
    31681 gtacataatc tcttcaaaat gtaggttttt tatttgtttt caacagagga gaggtcatat
    31741 tcgtattgct cataaatagt ggaatcgtta gtgataaatc gttttgccca attttctgca
    31801 tcataaaatt ccaatagctc tgataggatt ggcttaaatg gtgtatgcag aaattccggt
    31861 aattcatcta aatcacgttc tcgaagcaaa aaaatatttt caggacgata aaaatgataa
    31921 tgagcaatcg cttgattatc cgtcactagt ttcttttgaa aatacatgga ttcaaatgtc
    31981 cgcaaagtca ctccttgctg accctcccgc aacagttcca gaatcccgcg cgattcccct
    32041 gtttttttca ggtactcttc ataagtaaga tatccaggtt gttcattccc tcgatgtgga
    32101 agaatgaaat agttggtcgt aacatctctt tttataaatt cctgcttata gctttctgct
    32161 cgttcttttc tcccattatt cgttgcacca aaaaatagat ccgtctttgg ttcgctagaa
    32221 gaaaaattca tatgtttcga ataaaatgaa ctattatgtt tcagcccaag ttcttttgct
    32281 tccaaaaaat caaaatgata aatctcatcg attgccggtt catcttgtgc ctgcttcagc
    32341 atcgcctcgt gttctgcttt gaagtgattc caaaaaaata acacaatttt tgcattggta
    32401 aatgggcgca cgcgtcgagc catctccata tcaaatccat aatctaaaaa aacgatcagc
    32461 tgataatttg ccaactgttg tctagctgat ttaatgaatt ccgactgtag taaatctttt
    32521 ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat aaagtgattt attttttgca
    32581 actagcagta cgtcattttt gttcatcttt tccgcctcac tttctctgtc actttttcct
    32641 atgatagcct gtttaaatag taaaaagcca gtaaaacgtt tcctatattt gtatgaaaat
    32701 ttaacatttg tatgaaaata tgatgaaata aatcattctt ctattaaaat tcgcctaaaa
    32761 gttttctgaa tagttgtaat tgtctgaaaa tttgttatca ttagtcattg ttaaatattg
    32821 ctatttattc ttaatataaa ttttgattgt gaatttaaaa gaaagaggtc atttatggaa
    32881 gataaaaaat tgaaaaaaca acttagctcc agacatatca ccatgttagc tttaggtgga
    32941 gcaatcggtg caggtctatt taaaggaagt ggcgaagcga ttgggatcgc cggcccttct
    33001 gtattgattg cttttttgat tggtgggctg attttatata tcgtgatgaa aggtctggga
    33061 aaaatcgttt taagcggtgg cgacacgcat cacggattat ctggtttgat acgcccttat
    33121 ctaggtgctc attctgctga ttttactgat tgggtctact ggtctatgtg gattatcaat
    33181 atcatcgctg aagctgttgc agctgcttcc tttttgcagt tgtggttccc gcacattcca
    33241 gcctgggtat ttgtttttat gttggctgtt ttaactacga tcatcaatct ttattcggta
    33301 cggttattcg cagaaacaga atattggctg gcttttgcta aaatcagtgt cattatctta
    33361 ctgatcattt ttgctactta tcttgtcggt cagcaaatgt tgggcacagg cgttttccct
    33421 acccttcagc aattgactga ccatggtggg tttactcctc atggtatgaa gggaatcatc
    33481 agttctttgc ttgtagttat ctattcttac ggcggttctg aactgattgc tatcacggta
    33541 agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga
    33601 atcatttcct tttacatcat tcctttgttt ttgcttttga ttctttttcc ttggaatact
    33661 ttagcaggca cgaatgtcag tccttttgtg atggtatttg aaaaaatgaa tatcccattt
    33721 gcagcggata tcgtcaattt tgtgattgtc cttgctttgt tctcatctat caattcgggt
    33781 gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga
    33841 aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt
    33901 gttctttatc ttggtgtagc tctttcttat tttgtcggtg acaaattatt tggctatctg
    33961 gcaggttccc tttcgtatac agtattattg atctggatca ttatcagtgc ggcatccttt
    34021 gtactggcac taagaaaagg aacattatgg gataaaggaa tcagcttttt tgcattagct
    34081 gtcttaggat tgattttcct tggtatcctg ttcacgaact ccattggggt aactgtattg
    34141 actggcctgc tttacctgtt catttatttc agttatcaaa aaaagaatga tgcgttcgta
    34201 ttgacgaatg aatgaaatcg atagaaaatt ttattttatt tatgtggaat agatggagct
    34261 attgaagaaa ggagttgtga caaaagtctt gttgcaactc ctttttccga ataaacagtc
    34321 tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg caaaataatt gattgttctc
    34381 catgctcatg acatgataga agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa
    34441 gtattggaag tttttgttga tgatcggtgt ttcaacggta gttatgtttg gtatgatgta
    34501 cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt acacgagtat tcatggcgtt
    34561 gatgatgggt gcagtcatgg cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa
    34621 caaaaagata aatgttatga tcttagccgc tagtcttctt gtttttgccg gttccttgtt
    34681 tatggtacga agccaaacta ctgtcaacga cacagcttgg atgaaggcca tgattcctca
    34741 tcattctatc gctatcctgg caagtaaacg agctgattta aaagatcctg aagtaaaaaa
    34801 attagcggaa gaaatcattc aggcacaaga agaagagatc gacaaaatga aaaaaatgat
    34861 caaagaaatg gaatgaccat tatccgtcca aataaaaaaa gaggatgttc cagaagttat
    34921 cagcttcaag aaataagaag aagctacttc tggaacgccg tgaataaggg gttgtgaaaa
    34981 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagcttctcc tatttttcgt
    35041 ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtaa
    35101 aatcagcgtt ctgacctgag tattagagag acaaatggaa aaatagttcc tcatattttg
    35161 ccattttttg agctaatacc gagggtcagc tgattgaaca ccgtttatcg agaggttgtg
    35221 aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg aaatggcttt ccacattttt
    35281 gtataaattt ggcttaatac cgagaaactg gcttttgaat accgtttatt cgtaaaaggg
    35341 attgtgacaa aagtcttgtc acaatccctt tttatgtttt tattgttcta attgcgctgt
    35401 cttgcttact gcttcttgcg gtttcacaaa tttttttgag attccccaac ctgtgaaggc
    35461 actgaccatc gaaaacacag gagaaagcaa actgaaaaat acgaatggca agtaagtgag
    35521 tgtaggaata ccaaatgtac ttgctacgaa acttccggca attccccaag gcactaagta
    35581 gttgattact gttccaccat cttctagcgt tctacctaat acagttggat caaatcctgc
    35641 tttttcataa gtttctttaa atgcattacc aggcagaata acagatagga attgttcacc
    35701 aataaaaata tttactccga tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt
    35761 cgtcatcttt cttgatactc gttccatcac ggttgagatc aagccaaatt cgatcaacaa
    35821 tccacctaat gacaaagtta gaataatcaa tgacacagta ggcatcatac tttcgattcc
    35881 tccgcggcta agcagtgaat cgatttgttg gtttccagta tttgaaacaa accctgattc
    35941 gatgacccca gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac
    36001 tgctacagcg atattcaaca aaatcgttaa aattgccggc acctttttcc atgcacacag
    36061 taacattaaa aataaaggaa tcagtgacca gattgaaata gtaaagtttg cttctagaac
    36121 atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat
    36181 gaataaaaag aaagaaacaa caaatgccgg gacagttgac cacatcatat gcttgatgtg
    36241 tttgaacaaa tcggcatcaa caatagctgc tgctagattt gttgattcgg ataatggaga
    36301 catcttatct ccaaaaacgg cacctgaaac aatagctcca acaaccagcg ctggatttaa
    36361 gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc
    36421 actccctaca gcgctaccta cgattgcaca cacaacaaat acagaaggga caaaccactg
    36481 gacactgatc aatttgaacc caatgaccat tagagttgga atgatccctg cttgtatcca
    36541 tacagcaatc aatgaaccga ctaaaataaa aataaagatt gggatgattc ctggtttgat
    36601 gccattgatg attccttcgt gcagccaatc tgtcgggtat ctttttatca ctgcatacaa
    36661 catgaccatt ccgatcgctg ctaagatcgt gatattaggt gagaccttta atccgacaac
    36721 gccaatcgca atgcctaaaa caataaccat taatacaagt gttgcttcta aaaaacttgg
    36781 tgttcctttt tttcccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag
    36841 ttaagatatc ggattactcc gccgcaattc atccgcgatt cctcctttat aaaaataaaa
    36901 agacctgttt taaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc
    36961 tgtttttcac tttcaaactt ttattcaaat gtatggactt tccatgtaat tcgtcaatta
    37021 ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac cgctactgcg
    37081 tgaaaagttg acttgtacga agtttacctt ccataagatc atttgtcaat gaaaaagaaa
    37141 atattttttc atttttctga aaattatttt tataactttc acacacaagt ttatttttta
    37201 ctagaaagta gcgccctctt gtttttttct tgctactatt tcttgatgtt cttgcttgat
    37261 tctttctaat agctttggct ctttcaacag atcaagagct gtattggcga gtagttctgc
    37321 tgcaatcgca atagaagcaa ggcctttgtc acttttagca gcagctttga attcttctgt
    37381 gtgtccggca atatatgtct cagagattgc taccgtaggc tggattgttg gtatgacttg
    37441 gctgacattt cctacatcag aagaaccgag acttgttctt tgttccgttt ctatttcttc
    37501 atctggaaca cctgcttctt ttgcatgctg gaagaataag tcatcaaaag aaggagtaac
    37561 gatgacatca tcaactgcat tttggaacaa tccaaactca aaagtcgtgc ccgtacttat
    37621 cgcagcacct cgtacgatat tttccacttt ttcatatact ttatctaatg tttgccgatt
    37681 tcctgcacgt aaatagaaac gtccagcagc gtaatctgga atgacgttgg cagctactcc
    37741 tccgtctgta atgatcccat ggatattgac atcttttggc aattgcaagc gtagagcgtt
    37801 gattccatta aacacttgga tcaatgcttc caaagcattt actccttttt caggtgctgc
    37861 tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact ggatcattcg ctaaagattc
    37921 tgtcgttttc ccataacgaa aagctggatg gacgcataac gctacatcga catcctcaaa
    37981 aaaaccttct cgaacaaagc tgcctttagc tgatccgttc tcgccccctt cttcccctgg
    38041 ggtgccatat acacggattt ctccaccagt ttcatcaatg aattgcttca ctatgctggc
    38101 ggctaatacg gagtaagtac caaacaaatt atgcccgcat gcatgaccaa tacctggtaa
    38161 agcatcgaat tctgccaaaa aggcaattgt gggaccgggt ttattggaaa tataacgtgc
    38221 atcaaaggct gttcggtgtc cagctacttc tttttctacc caaaaccttt ctttttctaa
    38281 ttgtttaatc aacgctgctg aggaataaaa ctcatagtta cttacttcag gatgttcgtg
    38341 gatatctaga gcaagtgctt gatattctgg taaacgatat tggattagat ccttcaattg
    38401 gtttgttgag attgttgttg tcataaaaaa ttcctcctat gctagtcatt tttcttattt
    38461 tctcccgaat gtttcccatg caggcacgct tgatcctttt gatgttttct cgattacttt
    38521 gactgtgtct tctgtttgat aagcgtctac tattttttga tacgttttat tgttttcatc
    38581 ttcttttctt gcaacgatga tattcacata aggacgtgca tggtcatcca ctggttctaa
    38641 gaagatggca tcagaagcag gattaaatcc agcatctaca gctacaccgc tattgatcac
    38701 tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc
    38761 tagatttttt ctattctcgg taatatcaga aacagtgggc gcttgtcctt ttgccttatc
    38821 cactttgatc aatccagcag tttgcaataa cagaagagct ctgccgccat ttgtaacatc
    38881 attagggatt gctacttttg ctccgtcttt gatatcagct acatccttca aagtgttaga
    38941 ataaattccc agcggtgcat tgacagtatt tccgatcgat actaaatcag ttccgtgttc
    39001 tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc
    39061 tgctaaagcc acatttggtt gattgtaatc tgtaaatttc acgtattcca aattaatgtt
    39121 ctcctttttc aaacggtctt tcacgttgtc ccatacatct gtatcttctc caataatccc
    39181 tagttttacg atagtttctt ttttgtcagt tgtatctgct tttccttggc tacaccctgc
    39241 caaccaaata attgctacaa atgtaatgac tacaaaacga ataatttttt tcattttttc
    39301 tcctccactt tcaatgtgta atttttttaa tcagttgatt actgataaat tgacttaaaa
    39361 aaactaagat tagaatcaat aatgtagcca caatcgttac atctgtttgg aatcgattat
    39421 accctctcga tatagcaaga ttccctaatc cgcctgcacc tactgctcca gccatggtag
    39481 tcagtccgat cacgttgatg atagtcaacg ccgagacccg aatgatacca ggcaaacctt
    39541 ctaacaaata cacacgtaaa atgataccga ttggactggt tcccatcgct tctgccgctt
    39601 cgatcacccc aggatcaact tctagcagtg cgttctcgat ttgacgagca aaaaacggaa
    39661 caactccaaa aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga
    39721 aacgagtagt caatgctaaa agagaaagca agataatgaa agggatggaa cgaatgatat
    39781 tgatcagctt atcaagtatt tcaaatagca gacggttagc tagtaatcca ttagggcgtg
    39841 tcactactaa cgtcacacct aagatgattc caatcatccc gccaatgaat gctgtccaaa
    39901 aagacatata tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc aaataaacat
    39961 tgggcaaata tgttttaaag aaatcagtca ttttatcttc ctccttcgat cactttgata
    40021 cgactatcta aatcgttttt ttgtaaaata tttattttta caccagattt gatcaaataa
    40081 tccaatgctt ttttccgctg tttggtttct ccagctaaag taacgatcaa actccctaac
    40141 ggcgtttctt gtaataactc tacattgcca tataaaatat tcgctgttac ttgatatttg
    40201 ctgtacagat gagcgattag aggttcgttc gtctgatttc caatataact taattccacc
    40261 agccatttat tggcgatctg atcggcaaaa gcagaatggg caatgatcgt ttctaatgct
    40321 tggtcaagat gagtagctgt tcgaataaaa tctttggtca attcttcttc aggattgctg
    40381 aagatctgga tgctgctacc tttttcaacg attcttccat cttccataac cgctaccttg
    40441 ttgcagattt ctttgacaac ttgcatctca tgagtgatca ggacgattgt taaacctaac
    40501 tggcgattca atttttttaa caatgcaagg atctgaaggg tcgttttcgg atcaagtgca
    40561 ctggttgctt catcacataa taatacctta ggttcattcg ccaaagctcg tgcgattgct
    40621 actcgttgtt tttgtccccc cgacagttgt ttaggataag cagatgcttt ttcttccaaa
    40681 ccgacaagtt ccagtagttc attcactttt tgacgacgtt cctgtttcga ttttccagaa
    40741 tactttaaag aaaaatcaac gttgtcaaaa atagttcggc tttccattag attgaaatgc
    40801 tgaaagatca taccgatggc ctttctttct tttcgcaatt ctttgggaga gagctgactc
    40861 agattcgtct gattgacaat gacttctcct gctgttggtt tctgcagcaa attcatcaca
    40921 cgaactaacg tacttttccc tgcacctgag taaccgacga ttccataaac atcgcctttt
    40981 tcaatcgtta aatctacaga atctactgcc tgtatctctt tttctttttg tttgaaacat
    41041 acagaaacat ctcgcaattc aatcattaaa attcctcctt atctaaaaaa agcgtcctca
    41101 agaaaatgaa aatgtcattt tcttgaggac gcttttttgc gtgttaccac ctcatgttca
    41161 agctgtgttc acacacaact cctcttcaag tacgatcata ctctagcaag atatcggtcg
    41221 cagccgtcat cgccttacat ctatgctcgg gatgaattgc tcgatggcca ttttccaata
    41281 gtcgttcctt gcttcttctc agctaccgaa gctctctgtc aaaggttctt ctatcgtact
    41341 taccatttca atgcaaataa tattcaaaaa aaaagtcctt ttactaacat aaactatgtc
    41401 agtaaaagga cgacgtatcg cggtaccacc ttttttcgga agtttccttc cctcaagccc
    41461 aacatctcaa aaagagagat tggcgttcgt tttaacggtc gaacagttcc gaatttgcct
    41521 acttatcgac aaattattct ccaaggccat tttcagcttt tcttacttga ctcttctcac
    41581 cagccagagc ctctctgcaa agtttccaaa cttactttcc ttttcaacga aatcgatatt
    41641 ttgttgttgg gattaatcta catgaggaaa actgaaaagt caagtacgaa attcaaatta
    41701 ttttttctat atttattttt ctgttttttt atttaacatt tcaataacag ttcgaagatc
    41761 ttttccttcc gcaagaagtt tcaatttttc cttttcttcg acggtataat ttcggacaaa
    41821 actggaatca ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg ttgctcctac
    41881 taaagaatct tccactgcaa aggctgaact agcttctaat cctgttcgtt ccaaagctgt
    41941 cagataagga atcggtgaag gcttgttctc cgacacttcg tctccgaatg taaacgtgtc
    42001 caagtaatca gctaatttaa agtgatccaa gatatctgtt gctcttttcg taacagtggt
    42061 cgtagcaatg cctagtttca tatgacgttc ttttaagtct gccaatattt cttttgcata
    42121 tggttttaag tggatggctc cgcttgtcag ctgttgataa aaatattctt cacgcttcgc
    42181 tcggacttcc tgtacttttt cagggctacc tgctgctttc actaaaatat caaaactttg
    42241 tttccagttt tttccgcccc aagcgtcaac gatttctttt gggatatgga tatcaaattg
    42301 ctgaaatccc caaagccagc catcacgata cgttttttct gtgtcaacta acaaaccatc
    42361 taaatcaaat attgcacctt tcatctatac attaatcctt tccaaaacaa tacacatagt
    42421 ttagcacgac tggtccaaaa agaacagaag caaagaacca gccttctcgt ttataagagt
    42481 tcttggaatc gacggatata gattttcttc acagctgtca caagcgccag ataagcgatg
    42541 actgtagcaa ataaccataa ccagtaattg ccaggaagtg gcatcaagcc taattcagca
    42601 ccaaaacttg taaaaggaag gacagaaccg acaataattc ctaatgtagt gataacaaac
    42661 atggcaaatg ttgcgttgct ttggataaat ggtactttcg gtgtacgcag tgcatggagc
    42721 actaaggttt gtgtccatag tgattcgaca aaccatcctg catggaataa tgcgatgaaa
    42781 atcactcgct gtgaactatc caatgtatgg aaacttccgc ccactacagc aggacaaata
    42841 acaaaataca tcaacaagta agtagtaata tcaaatatcg aacttgttgg accaaagtaa
    42901 accatgaatt ccccaatact agatgcattc catttttttg gctcgtccaa atattcttta
    42961 tccatccgat cccatggcaa ggaaatgcag gaaacatcat agatcagatt caaaaacaaa
    43021 agttgtaaag gcagcattgg taaaaatggc aggaacgtac ttgcaatcag cacggaaaac
    43081 atattgccaa aattagaact tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt
    43141 tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa aaggatcacg
    43201 tctgcagatt ctttcgcaat atccacagct gtatctacag aaataccgat atcagccact
    43261 ttcattgcag gagcgtcatt gataccatct cctaaaaatc cgacagtatg accattttta
    43321 cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca attttacgaa tacttcatgg
    43381 tcttcagcta cttgtgctaa ttcatgatca ctcatttccg agattttctc accggtaacc
    43441 aattcattga tctctagacc tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc
    43501 ccagtcagaa cttttacagc tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt
    43561 gtttcttttg gtggatcaag gaaagcaaga taaccaatca aaaccatgtc tgactcatct
    43621 ttgactgaaa attctccaac gacgcttgga ttggtctttt gtgcgacacc aatcactcgt
    43681 aagccatctt cattaagtga ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt
    43741 aatggtgtaa cagtaccgcc aagatcaata aatttagaaa tgttcagcat ttcttcaatc
    43801 gcgccttttg tgatcatttg tgttttacca gctggatcag caaccacaac actcatacga
    43861 cgacgttcaa aatcaaaagg aatttcatcc actttttgat aattgatctt atctgtatct
    43921 aaagtttctg ttgcttcatc aatgatagcc acatctaaca aatttttcag ccctgtttga
    43981 tagtagctat ttagatacgc atggcgcaaa acacgatcat cttcatttcc ttcacaatcc
    44041 atatgatatt caagaatgat tttatcttgt gtcaatgtcc ccgttttatc cgtacataac
    44101 acatcgattg caccaaagtt ctggatggca ttcaaatttt tgatcactgt tccttttttg
    44161 gccatcacac ttgctccttt gaccaaattc gtcgtaacga tcattggaag catttcaggt
    44221 gttagaccaa ctgctacaga tagtccaaaa agaaaagctt ctagccaatc cccttttgta
    44281 agcccattga tgacgataac tgctggagcc atcaatgaca taaatttgat cagtagcatg
    44341 gatgttttat ggatgcctaa ttcaaagctt gattgaattg gtttttcaga tagcgtttct
    44401 gctacatgac caaatagtgt ttcatttcca acagaaacaa tgattccttc cgcacttcca
    44461 ctgatgacat tacttcccat aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg
    44521 tcttcacata ctttcgcatg cttttctact ggatagcttt cccctgtcaa tgctgcttgg
    44581 gaaatgaata aatcctttgc ggacaataaa cgcatatccg ctggtatcat atcccctgct
    44641 gaaagccgaa caagatctcc acagacgatt tcttccatag gaatctctgt gtactttcca
    44701 ttacgtttta cagctgccgt tactttcaca agtgatttca gtttttcagc cgcttgattg
    44761 gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga taaccatagt gaagataatg
    44821 gctacaccga aaagatcttt atctccagga gctgctaata catattccgt gatgaatgaa
    44881 ataattccta atgcgatcaa cactaatgta aatggcgtga tgtatgcttt caatatttct
    44941 acaatgaatg gtgttttttt gccatatgaa atcgcatttt caccgtaatt ttctcttgaa
    45001 cgttcgattt gttcttctgt caatccattt gctgatgcat ggaatagttc aaataattct
    45061 tgtttcgttt ttccagcaaa agctgcatag attttttgtg tatctttctt tttttccatc
    45121 tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc tttagctatt
    45181 catcggatcg atagagaaaa aactgaaagg ggctgagaac agatagataa tagccctggc
    45241 ctttcctcta gatccttgtt tttcgaatga ctgtcatcca tttgttctca tctcctttac
    45301 taagtaattt taattcatga cttccaatca tttttcgaat agttattttt ccaattagac
    45361 cacctccttt ttatttttca ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa
    45421 tactcagcga aaggtttgaa aaagcatatt aaaaaaagct tctcagatct catcgttgag
    45481 ttttgacact gtacaacgta aaaagcagat acctgctttt agctacctta tgaaaagcct
    45541 tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg cagtagccta
    45601 tgtttgtata ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt aatataacaa
    45661 ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tacttttttt aaaatacaca
    45721 cgttatattc ctgtctcacg cggttttatt tgtctcattt tccacctccc gctataatag
    45781 aaatatagta ggaaacgagg tgctgttatg gaaagaacac ttgttattat caagccagac
    45841 ggcgtgagac gccatttagt cggaagcatc atccaacgtt ttgaagcaaa gggactggcc
    45901 attgctgaaa tgaagtttga aactatgact cctgaattag caaaagaaca ttatcagcac
    45961 ttgactgagc gcagtttttt tgatgaactg atcgattaca tgacttccgg tcctgtcgtt
    46021 tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca
    46081 aaagctgccg atgctgttcc aggtacgatc cgaggagact acgcgctgcc ggggactgaa
    46141 aacatcattc acgcttctga ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc
    46201 ccgccattcg atataaacaa accgtctgaa aaagcggcga agtgatacaa attctaaact
    46261 gctggtctag ttgctgggtt cagcaatggc cagcagtttt ttctctaagg acctccgttg
    46321 attctttcct aacatatatt cgagcactgt gaagaattct tggtagtctt tcgtcttttc
    46381 ttcccctaaa gcagcagaaa tcaatctttg ataataatct gccatcaatg tttcatagat
    46441 cactgtactt ctttctttat gtgataagct ccgtttcata tcttcattca cactacgtgc
    46501 ctttttcaac cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag
    46561 atactgctca tgattgttca actgtgctaa tcgtcgcatt ctttttttga ttttcaaata
    46621 attgttctca atgaaccgat cactgaatat ccaaggaaaa tcagctagaa tagatagttc
    46681 aaattgacca tagtctggta tctgcatcaa gtattcttca atgattcggc attcataacg
    46741 taaaaattca aattctgtct cagatacttc tgttttttcg accatgtgaa gattcaaaac
    46801 caaataatac agatcttctg ttttcttaaa atgcctacgg ttattgctga tctctttata
    46861 aggatcaaag ctgtcttctt gtacccgatc tagcattcgt ctttttttct ttaactttat
    46921 tggttcatcc agctggtttc gcatccattc atattctgca agtgtcatgt tcattcgctc
    46981 taaaaatgat ctcaatgtta caaatgacat ttttttcact gaatttgtct caatcccgac
    47041 tagagaagtc ttaaaacaga ttccttccgc taattgggac tgtgtcaact tcttgttgac
    47101 tcgaagtttc ttgatcaatt caccatcata cataatagtt ctccttttta atttaaattt
    47161 ttccacattc aataaataga tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat
    47221 gtttttttca taaaaaatac caaccttcaa gcattagaat gttgtctaac tactgaaggt
    47281 tggtttgatt gtttattgat tatttttttg ctgttcaacg tctgttcttg ttactggata
    47341 taagttggtt gttgttttat taccattttt tgaaaagaca cttgtcgaat gttcgccttt
    47401 ttgcttttcg attgcttcat tacgttccgc ttcattttta tagtcatatt ttttcggatc
    47461 aatcgtctca aatccgtttg gcacataaaa ccgcaagaga ttttcctgat tcagcttatc
    47521 tgaaagactt aacgcagtct tgactttttc ctgatctttg gagatttcct catcgattcc
    47581 tgctgctttc gtatcgatga tttctcctgt ctgattattg taagcttttc ctcctaggac
    47641 ggtgtatttg ggtgtcacaa agttcccatt tcgaaaagca actacttgat catgctgaga
    47701 agataataag tctgtaccaa aatgaagata atctttatca tcgattccca atagatgtag
    47761 taatgtcggc aaaacatcga tttctccacc gtattcatga ttgactgtcc ctttagtata
    47821 accaggcata tggatcatca atggcactcg ttgcatctgt gcgttgtcaa attcatccca
    47881 tgtatctgga tctttaccaa gagcgcttgc taaatcttta ttatctgtat tcgagatccc
    47941 aaaatgatcc ccgtaaatca caaagattga attttggtaa ataccagatt ttttcaaata
    48001 cgtaaaaaat tgttccaatg attgatccag ataatgtgcc gttcgaacat aattatctac
    48061 tgtgctattg ccagtattca aagatggaaa gtcgaaattc ttgtcatccg tataataagg
    48121 cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat gctcaagata
    48181 ttgggcagat tctctgaata aatctttatc taatatccca taacctaacg tttcgtcgga
    48241 ttcatcaaaa taagatcgat caaagaaatt gtcataacct aagtttttat atacatgatc
    48301 tctattccaa aaactagcta cattcccatg gaaaacagcg cttgtatagc cttgcttttg
    48361 tccaaggatc gctggtgcgg cttgaaagac attgtctgac ccaagttctg tgaataatga
    48421 gccttgaggt aaaccaaatg tgccagtttc caacatattt tcagcatcac ttgtctttcc
    48481 ctgtcctact tcatgaaaga aattgtcaaa gctgatcgtt gcttgattct ggaaaatact
    48541 atttaaaaac ggtgtgactt cttgtccgtc taccttcata ttaatcaaaa actgctggaa
    48601 actttctaaa tggagaacga tcacattttt ccctttagcg atcccaaatg tttcgggatt
    48661 tggctctgca taatgtttct ttgtataatc taaaacttca tcaatcccat tactgcttgc
    48721 atgcgcgcga acactgctag tcattcctgt tttgattccg tcataaatag tatacgcatc
    48781 gattcctaga tatttgacaa tatatgaacg gtcaaatgtt cgctgcagca attgtggacg
    48841 gttggcttcg ctcagcgcta gatttcctga aaaaataagc cctgaaagac aggtaacagc
    48901 gatagccatc ggtttgccga cagggttgct tttgataggt attttcttga ccttcaacca
    48961 aaccagtagc ccaatgaaca cagcgatatc cagccagtag atgacatcat ggggcttcat
    49021 caaagaaaag gagcttcctg acaatccttg cgacactttc gaaaagccta atacagattt
    49081 gactgtaatg aaatctgtga attcccgata aaaaataata ttcaagtaca acaaaagcgt
    49141 atttaaaata tcaatgatca aaagaatagc taacgctggc tttggctttt taatatacaa
    49201 agaaaggcca aaaaacaata aggtagtcgc aattgggttg atccacaaga taaagtactg
    49261 gatcgtacct tccacaccta atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca
    49321 gaaaaagaaa acagctgtca aaacgattgc taaacgattt tggcacaatt ttttaagttg
    49381 tgtcacgtat atcacttctc tctattcaca ttttttctta ttttgctcct gaaaaatgaa
    49441 aatttcctaa aaaagttgtt atcttaagat ttttttattc tttctaaaga ttattccgtc
    49501 cacttttcac agcataaaac cgctgcgatg taagcgatta aactaatgaa gagataaaag
    49561 tcttgtggtg accgcttcac tccacggtat tcaaaagcca gtttctcggt attcgtacaa
    49621 aaaaagaggc tgtgacaaaa ttcttgtcgc agctccttat ttcttgatgc aggacagctt
    49681 tttcacaact tcttccttaa acggtgttct cctgccaact cctcggccac aagtcacaat
    49741 atttataaaa catgagaagc tgttttttga aattgttcct tgtggctcag agttaaacgg
    49801 caggctcaag acctcttgat acacggtgtt caatcagctg accctcggta ttagattatg
    49861 tcaggacgct aattttacaa cctcttggta aacggtgttc aaaagcttgc atctgcggta
    49921 ataagcccaa ccaaacgaaa aatataagga gctatttttt gaaattgctc cttgtggctc
    49981 agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccttcgg
    50041 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
    50101 caggtcaaaa cgctgatttc acaacctctg tttttcattg tccataggat tgatattgtc
    50161 cttgtgatga ctcattcgta ataattgagg cttgattttc ttcacttccc gttatcgttt
    50221 gtccactgcc agaatctgaa ggacttgttc ctgtcgactc gtttttttga gaagagacag
    50281 ttgttttctt gacttgttca tttgtctttt tccattccgc taaacgatcg gcgaccactt
    50341 ttttgtctac tttttcagaa taagcggtga tcacttttcc atttttcaaa cgggctactt
    50401 tgtcgccatc atgagtcaaa agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc
    50461 tgagagaaat cgcactaagt acgtcatcga tattaataaa ttgattttct tcttttcctt
    50521 ttggcaaata aatgtccaat tcgccttttt cgtaattggc agttatttta tactccgccc
    50581 accaataagt tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt
    50641 tttctgtttc tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa
    50701 taagcagtaa gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc
    50761 tttcctccac tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag
    50821 acaaaaaagt tgaaaaagat cgtaaataat tgaacttctt tttcacaaaa tggtaagaat
    50881 ggagaatttc acgcatttgc tctcaactat tctttcttaa gcctcaaatc cgtttttatc
    50941 tgatacgtct ttgaaccaat gacccgattt tttgattgtt ctttttccat cttgtgctaa
    51001 gtctactgaa ataaagccat atcgattctt atatgcattg gtccacgacc agttatccat
    51061 acatgtccac atatgatagc ctaaacaatt ggcaccttct tgaatggctt gatggacata
    51121 tttcagatga gattggacaa attcgatccg ataatcatcg tggatcatgc catcttcttt
    51181 gataaatcgt gcttcgtttt ctacacccat accgttttca gaaataaagc agtcgatatt
    51241 tccataatga tctcgtacat tgatcaaaat atcatagatt ccttgttcat agatctccca
    51301 tccgcgataa ggattcattt tacgaccagg catttcataa ttgtcgaaat aatcctcagg
    51361 cattggaccg tttttagtat cgattggtgt ttcttttgct tttacccggc gtggctggta
    51421 ataattgata cctaaaagat ctactgtatt ttctttgata accgtcaaat cctctgcatg
    51481 gatcacaggc atgagatcaa gttcttttac tatctcaatc aattcacttg gaaactcgcc
    51541 ttttacagct ggatcaagga atgaacgatt gaagaacgca tctgctattt gcgctgcttt
    51601 aacatcttca gggtttgatt cgtctcttgg atagcttggt gtcagattca agatgatacc
    51661 aataacacca ccttgctcca tttcatggta agatttgatg gctaacgctg aagccaacgc
    51721 ttcatgatag ccaacttgga tcgctttttt catatctgac tcttctggat aatgccatcg
    51781 atataaataa cctgcttcga ctggaacgat tggttcatta tgagtaaacc atttttttac
    51841 tttcgaacca aacaattcaa aacaactttt ggcatatttt tcataggctt caaccgtttc
    51901 tcggttcaac catccgcctt tttcttgcag atacatcggc atatcgaaat gatacagatt
    51961 cacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac
    52021 ggcttcttcg ttgattgttc cagattcatc aggtatcaaa cggctccatt ggatagatgt
    52081 tcgaaatgaa ttatgcccag tctgtttcat caactgtaca tcctcttgat acttcgtata
    52141 aacttgcgag gttttttcag gtcctacttg atcaaagaac ttttgcggtt cttttttgta
    52201 ccaataatca aaaatgttct ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg
    52261 gccacttgct gcagaacccc accaaaaatt tgctggaaat tgatattttt tcatcaaagc
    52321 acctcttttt cttttttagt tcgttttagt tgattccatg tatgatattt tccatcacaa
    52381 tcactttttc attttcttcc tcttcttttt tgattcgttt taacagctgt tctgctgctc
    52441 gttccccgat tttatctgtt ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt
    52501 ttttatctaa ataatcatag tgactgaacg caatatcctt cccgattctg acaccttctt
    52561 gattgaaaca ttcgatggca ccaatcgaca tattgtaatt gctgacaaaa atagcagttg
    52621 cccctttttc taataatgct tttgccccgt aatagccgct ttttctagaa tagtctcctg
    52681 taaagacctc taattggatc gatggatcat tttggaccgc ttctttggct cctgctaaac
    52741 gttctcttgc tacgtagtct gtttgagtgg cagcaataaa tccgattttt ttatgtcctt
    52801 gttcaccatg atgagcgatc acttttgcta cactttctcg atcattcact aatattgtat
    52861 caatacctgc tggtccttct ggacaattca atgcgataac tggaatactc atttttgtga
    52921 gcatttcaaa tccagaccac tcttcttcgg ataagaagac aatcagccca tcgatcacat
    52981 gcttcatcag gtattcaatt ttttgttcaa ttttttctgg ctgatcggca aacccgctta
    53041 ataataagct atacccattt tcttcagcta ctctttcgat ccctgagaca acttccgacc
    53101 caaaacggct ggaaatcccg tttatcaaaa gtccaataga gaagcttcga ttattcttca
    53161 accctttagc aatgatgttt tctttatagt ccaacgctga aatcgcttct gctatttttt
    53221 tcatgttctt ttccttcagt tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa
    53281 caccagccat cttagccaca tcacggatcg atgccataca ttctcactcc tgccagttac
    53341 atttgatttc tctttcttgt tgttcgatta tgatgaccgc attctttgca tagtgttgct
    53401 tgtcatcaat atccatataa ctaaagtctt cagtagagca acttcctctc actattaatt
    53461 atacttaaac aagtatttga accggttcat ttttaatata gcattttttt aaaagattgc
    53521 aacacttttt taaaacgctt tctgaaagtg gcggattttt tgccttttta aaattttatt
    53581 ctaaaattta ctctatttgt gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt
    53641 ttacattcca atggtataat ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat
    53701 taatcgtact atttattttg tctgcgggac ttttgacatt gactggctgt gatttgtttg
    53761 gcggctctga ccaatcctcg gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag
    53821 cagataccac caacagtgta tatacgctaa aagtacgtaa agatatggat ttatccgcgg
    53881 gacagttgac atttacagat aaaaaaatgg aatggacacg cacgtatcag acacaaggaa
    53941 ctgattcttc tgaatcatct gaagaaaaaa cagtgctgac agatatcaag atcactacca
    54001 aagatgacga atatattatt tcaggaaaag aaaataaaaa agaaacaaca gtcatattta
    54061 caaaaatcgg taattaccgt ttgaaggatg aggatggcaa catctacagt ctatagtgcc
    54121 gtctgttgga tcttcttttg tttctcccct tcttttctgc tatagtaaag aagcaagatg
    54181 attgtaaagg agacgaaatg gatgatcgat agtcctgatt attgtacatg taataacaac
    54241 aaaaatatcc atacagaatt taatgaccaa ggaagcttga gcatatgcga caactgtcac
    54301 aaactagtca aagattcatt taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa
    54361 agtagaagaa gataaacaaa tccaagcaaa actcactatg gctgattttt atgaacttca
    54421 aactcagctt cttcctttga tcaagcttca tcagtaaaac aaacagtgga tcatttcagt
    54481 ttacaaatca aagagtaaag atttatttat ttcaaatatt tgaatataaa ataaccaaac
    54541 gatagaaagg cttattctac atcacgtagt aatttttctt tcccgtcgtt tggtttttta
    54601 tttttagcct aattcttgac cttttgcatg ctggttatcc gtaaaatatt ttccagcagg
    54661 ctttccttcc aattcagcta atttttcttg gatctgacgg atcacctttt ttgcatcttc
    54721 tggattttct acaaaatcga atttatcccc atccacttgt attttaggac atacatcaaa
    54781 atcttcatac cattgattat agcgggtgtt caacgtttca taataggtat ataacgtctc
    54841 atcgctttct agctgttcat agtcacgccc acgttttttg atacgttcta gcatcgtgtc
    54901 caaagaaact ttgatatgga ccatcaaatc aggccgtttt tttggagcaa catcatctaa
    54961 ctcgtttagc atcgtatcta ataagctatc gtactgctgt acttcgatat ccgttacccg
    55021 acctaaatca gcgtttagat ggaacaacag gctatcttca tagatggatc ggtctagcac
    55081 attgtcatcg ttcactaaag catttttcat tgctaaaaat cgcttgttca aaaagaagat
    55141 ttgcaataaa aaagcgtact gctcaggatt tgaataaaag agcggcaata cttcgttatc
    55201 ttcgacattt tcataaaatg gtttagaatc aatctctttt gcaagcatat ccgtcaaact
    55261 gctcttacct gcaccaatcg ttcctgaaat caaaatcact gacatacaac ccttcctctt
    55321 ctataagtac tcgcctacgt aagtttcaac aggcaatgtt tcaaaatcat attcacgtac
    55381 agcttggtct gattttaaat acgtatggag actttgtgta atcatcaagt tcgtaggtac
    55441 cgtctcttct tgtacaatga tcatcggttt tttcaacata gttgccacac ctaattcgta
    55501 agccgttcct ggatcgattg tttgatgctc aaagtcaagg atagcaacaa caaattctgc
    55561 atttgtcagt tcttccatat ctttttcgta tacttcctgc gcccatccag cgctgaatag
    55621 ttcataattg ctttcttggt gatgacgtgg agagtagaaa cttgtgactg ttttgttttc
    55681 ctccaacgct ttttcgatac gacttactcg atcgatttgc tcctctgaaa aaaatggtcc
    55741 tgctaaatat atttgactca ttattttgcc tccttcaata ataactgatt tttaatagat
    55801 actgctcgta attcattgac cagttccaat ggattgttgg aaaagattac ttgatccaac
    55861 cattttttat ccataaaatc ctcttttgca aaagtttcta ataattgttg caagccgctg
    55921 taataaccgc caatattcaa tattccgatt ggcttgtcgg gcgtcagacc gattgctatc
    55981 caagaaagaa gttgtgagaa ttcctccaat gtacctagac ctccagggaa aacgaggaca
    56041 gcatctccct tttcaaataa taataccttt ctttcatcga tcgtttctgt aggaataaat
    56101 gtagtaacat cttttccagg caattcagtt tcaaaaagcc cattcggata gatacctgtg
    56161 acttttccgt tattttccag tactgtttta ctaatcactc ccataatccc ggagtatgaa
    56221 ccaccgtaaa ccagttcgat ttcttcttgt gccatgtatt ttcctaacgt ttgcgcaata
    56281 aatttgtaac tttctttatt accaaaacgg gaaccgcaaa aaacagtaag tttcataaaa
    56341 aaacactccc taactcaaaa gtctatcttc attctacacg tgttttttta aaataaaaga
    56401 ctagattttg tagtttttta gtggtatata ggatagacgc cactatatat agtatttaga
    56461 aaaaaatcat ttttcagcca ttttatagat aaaacaaaaa taaataacgt gttttcatcg
    56521 atatgaaatg acttacttat agaagatact gggatttcgt cctatatgcc aataacgtgt
    56581 attattttaa acaacaaata cttgtttctt ttattaacga aaaaatcatt ttgttctcgt
    56641 gttatgtttc actgataaat gatgtttctc taaataaaaa agccgacgaa ctaaacgatc
    56701 aaaatttgat catttttgtt catcagcttc tcctaaaaat ctttttgtta acgataaata
    56761 gctggatttg taaaaatatc acttgaatcg tcactgctgg aagaaaattc agtgataacc
    56821 gcacctgttg cgcctgcctg gaactagtgt ttgatattgg gctcgaacgt gtactgatct
    56881 ctagcatgaa gaataatttc tttttttact gtataataac tttctttcct ttttggaata
    56941 gtacaggaaa gtttttttgt ttcctttcct tcgacataag gtataccaga ccctttcgac
    57001 aacgaaaggt tctcattttt ccttcatcgt catttcaagg agggtgccga tgctctggac
    57061 agtttcgatc gctcctaaac caaaatctgt atattcgatt ctttctaact ctgattcttt
    57121 cataccattc ctgtctcaag gtacatctat tgtacttttt tcttgtaatc cattctgcct
    57181 ctcctaacta attgtcaaat catcccaaga tatgtcttct tcctcttttt cttcttggat
    57241 gatttctgca ttcggatcta ctggtttttt caataataaa atggttattc ctgtgacaaa
    57301 agaaccagct aacaatgcac caatgaatgc ccatggtctg gtcatcgtag cgactgcaaa
    57361 cattcccgat gccggaatac ggctatctgc gccccaaaac atgcttaatc cgccacctac
    57421 tgtcccaccg actcccgttg ctatgacatt gcgtgccaag tcgttcaata cgatagggaa
    57481 acatccctct gtgatctgac agatccccat aggaaaagcg gtcttcaatg tatcgatttc
    57541 ttgacgattg agtatatttt tacgaatgat tttccctaag aaataagcga tcgtcaagcc
    57601 gaatggggtt accatcgaag ctaaaatcaa tacagtgatt ggttcataga ttccttctga
    57661 aaatcgtgcc acaccccctt atccagaata aatggtaatc cagaaacagc cgtgtatctg
    57721 cggtaataag cctaaccaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat
    57781 tacttgatgc agggcagctt tttcacaacc tcttttttta tttatgtttc ataatcaata
    57841 tggtaaggat gcagctccgt atggtagacc gcctccgaat attcaatcat ttccccaatt
    57901 tgcgtagacg atttccgtat ccgtttgatg ccgatctgtg cgtctgtttt cagaattcct
    57961 tgttcttctt ctgttaaaaa tactgccgag aaatcatcag aaaatgagtc aatagtatat
    58021 ccttttttcg tcagcaaacg atacaaagat tctttgccaa attcttctct gctgaattgc
    58081 atcatttctt gtggaagata atgaacaaaa taaatataag gctgattgtt caaataatat
    58141 aagcgcttaa aacaaacagc ttgctcaccg aaaaaggaaa acaaaggatc ttccttcttt
    58201 atatggacca attgcaaatc cagcgtttct ttccggactt catatcggga gttctccaag
    58261 atttgcgtaa agcttgccgc tttggataat cgattgtaag gacgattgct tagaaccgtc
    58321 gtcccacgac cacttttctt tgcgacatat tcatctgatg ccagcagttc gattgccttt
    58381 cggacagtga ttttgctgac ttgaaacagt tcttctaatt cactttctgt cggcaacatc
    58441 gaaccgactg gatattttcc tgagaaaata tcttctttaa ttttatctgc gacttccaag
    58501 tatagtacac gtgtcctttt cataacgtca ccctttgctt tactattact aaaagaatag
    58561 cacataaaaa ataaaaaaag aacacaaatc acatgtgaga agtgtgttct ttttcattca
    58621 gctgctttaa ttacaagttt acgccatttg aggcaattac ttctttatac caatcaaaag
    58681 attttttctt tgatcgattc agtgtcccat ttccttcatc gtctagatcc acatagataa
    58741 agccgtaacg tttactcatt tctcctgttg atgcactgac aagatcgata catccccaag
    58801 gcgtatagcc catcaaatct acaccgtctg ctactgcatt agccatcgct tcgatatgac
    58861 gacgtaaata atcgatccga taatcatcaa taatcgtgcc atcctcttcc atatgatcaa
    58921 tggctccaag accgttttca acaatgaata atggtttctg gtaacgattg tataattcat
    58981 tcaatgcgat tctcagccct tctggatcga tctgccagcc ccattcactt gcctcaagaa
    59041 acggattttt tactcctccc agcaagttcc cttctgaagt tgctgcttgt ggatcagtct
    59101 cattgattgc cgtagacata taataactaa atccgatgta gtccactgga tattctttta
    59161 gcaactggaa gtctttttcc gtttgctcta atttttccgg atcgactcca tattttttat
    59221 gggtacgcgc tgtgtatgca gggtattctc cacgtacttg gacatccgtg cagaaaaaat
    59281 tgaattcttg gttttggatc attgttgctg cctgattgac tggattggca tcgatgctgt
    59341 aagttgttgc atagataatc atgcagccga tttggatatc cgaacgcaat tcgtggccga
    59401 tttttacggc tttgctactt gcaacgaatt gattatgcca tgcttggaaa atattttggt
    59461 actctcctgc tccattgctt tttactaatc cttgacttaa agctggaaag tgaaaggccg
    59521 aattgatttc attgaaagtc atccaatatt tgactttact gctgtatctc tctaatactg
    59581 tctgagcaaa tcgttcatag aagtcgatca gttttcgatt tttccagcca ccgtattctt
    59641 ttgccaaatg aagaggcatt tcgtaatgag aaatcgtgat cacaggttca atatcatatt
    59701 ttaagcactc atcaatcagc tgatcataaa attctaatcc cgcttcgtta ggagtacctt
    59761 cgtctccatt tggaaaaatt cgtgtccagg caatcgaaaa acgataacat ttgaatccca
    59821 tttccgcaaa caaggcaaga tcttctttga aacgatcgta atgatcgatt cctcgatgat
    59881 tcgggtaaat atatttttct tgatcaatcg tccagtcaaa ttcttcactg ccgataatcg
    59941 caaagcgctg cttacctcca ggcatagcat ctgcaacgga tagtcctttt ccgtctcgat
    60001 cataagcccc ttctaattga tttggcagca gttgctccac cccataaaaa tttttctgaa
    60061 aaacgtgatt gcatcctgat ttcctcctta ttttacaatt gccagaacat cttcgtttgc
    60121 aataatctct ttttggtcta attcaagtat atcggcaaat gcatcagtat tcgtaatgac
    60181 aactggagtg attacgctat agccagctgc tctaattgct tcgatgtcaa aacgaatcaa
    60241 taaatctcct gctttgacga gatccccttg tttgactaag atctcaaagc cattcccatt
    60301 taattctact gtatccatcc cgatatgaat caaaatctcc gctccgtcag tcgttgtgat
    60361 ccctacagca tgtccagttg gaaaaatagt cgtgatctct ccgtctgcag gtgcaactag
    60421 ttctcctact tctggatcaa tagcgatacc ttttcccatc acgccagaag aaaaaacttg
    60481 gtctggtact tcactcaatg gcagtacttt acccgttaaa ggacttttta aggtatgccg
    60541 tgctgaagta atcggttctc ctgcattcgc atgtttgttt tctaattgat tgtcttgtgt
    60601 ttgttcgtca aatcctaaaa tcaatgtacc tacgaatgca agaacaaaag caattcctgt
    60661 agctattact gctactgtca catttgactc tacaccatcc accgtattaa tgaaagtcgg
    60721 aatcgtcaat aagctgacca aactgctgga gaaagctttt actccggaaa atccgataat
    60781 agctccacca atacctccag aaatacaagc ggcaatgaat ggtttcttca aaggtaatgt
    60841 cacaccatac acagttggtt cagtgattcc aaataacgaa gtcactgtag aagaaatccc
    60901 taatgctcga agtttcgtat cttttgttcg caatgctaca gctaaagcag ccccaccttg
    60961 tgccaagatg gctggaagaa gcattggcat caatacatca aacccatatt gttcgatatt
    61021 caaaaacatg atcggtacga atccccaatg catgccgaac atcacgaata cttgccataa
    61081 acttcccatg attagacctg ctacgattgg actaaatcca taaatcgagt tgaagagacc
    61141 gcctaaagca ttaccggcaa ccgtcccgat tggtcctaaa gcaagaaatg ttaatggaac
    61201 catcactact aaaacgacaa gcggaactaa aatcatttgc agaaattgcg gaatcacttt
    61261 ttttacgaat ggctctaatt tactttgaac ccatacggct agaatgattg gtatgacaga
    61321 agaagtataa ccagatggtg aaagaatcac tgggatccca aagaaactga tagcttctcc
    61381 cgcaccagca actgctgtga tagctggata cacaagtgcc atagcaattg ctactgctaa
    61441 aaactggttg gctttgaact ttttagctgc tgtaaaagca agcatgacag gtaagaaggt
    61501 aaagacacca tctgctgcag caaacaatat ttgataagta ccgctatcag ctgtcagcca
    61561 gcccatcacc gttgccaatg ataagaatcc tttcaatacc ccggcggctg ccatcgcccc
    61621 tagaaaaggt gtaaatatac tagagataat atcgatcaga cgattcaaaa tatttttgga
    61681 ctcttctttt ccatcctccg aaactgcaag accttcctca tccacgatcg attgataaac
    61741 atctgctaca ttactgccaa tcactacttg atactgaccg ccactttgta cgacttggat
    61801 cacgtccggg tcttctttta acttttgcgt atctgctaaa gattcatctt ttaatctgaa
    61861 tcgcaaccgc gtagcacaat gtactaaact tttgacattc ttttgtcctc ctaccgcttc
    61921 ccatacacga cgaccaactg cttgattatc cattgttttt tccttctttc ttttttattt
    61981 aaaaaataaa aacccagatt gaaaaaaaca gtcacaaaca ttcgtgaacc tattttttca
    62041 atccaggttt tgcctgccat acagtaacaa tccttatgat tggatgcttt ttttctcact
    62101 tactactctt tgaatatgaa tcgttaaata aatctgctct gcgatcccca tttcctgttt
    62161 ttgcgtttgt cgaagatact cattgatccg ttcgacacaa tgaaacgctt ttggatactt
    62221 tccttgaact aatgcgtaga gaaattcatc ttgctcgtca gatccttccc ctttgagata
    62281 acgctgggca aaaaactgca gatgagtaat gatacgctga taattcaaag attcttcatc
    62341 gaacacgatg ccgaagaatt tactgatgat actcagtata tctcttacca ttgtggtagc
    62401 ggtcatcgtc acttgcatgt cttcgtttcc ttgttggcta ttcacaaaat gaaaggcaat
    62461 aaaaccggct tcatcctcag aaagttccac acccgtcttt tgtttcacga acaaaagagc
    62521 ctctttcgct gcttgatatt ctttcggata aaattttttt gtttcaaata ataatggatt
    62581 aggcagttgg atattttctt ttgctcgtgt gatcgcataa tgaagatgat cggtcaatgt
    62641 cagataaata ttaggcgaaa attcaacttt tagtttatct tcagccagtt cgactacttg
    62701 tttgacccat tcgatctcct ctgccggaat atcagccatc agctgctgga attggttttt
    62761 agcaaaagaa tctcttaaac aaattctttt tggaccgctt ctggcgaaat ctcgtcccca
    62821 atcttttttt gaaaagctag tccttttccc atacaaacga cttcttcccc gaatccattt
    62881 ttcgaaatga cgacattgtt attcaaaatt ttatcaattt tcattttgct tcgccctctc
    62941 aagaaaaata aaaaaaccta gaattcgcct actgtttctt agctgaattc tggttttgcc
    63001 tgccgtacag taacaatcca tttcttattc aaaatcatca taccatcgaa tttttattta
    63061 tgcaagcctt ttcatataaa aaataaaaaa actggaacac aagtctatct agaaaacaac
    63121 caaatcagag atgcacctct ttgcgtttct ctttttttca gcaaattggt ttttccttat
    63181 cgattcatat cctttggctg tttttaatct gtaacgactt atgtcccagt atcctaaaat
    63241 gtttttgtct ctagattgaa agcagatctc atatcatctg tcgcatcttt tatcgatgta
    63301 acactttcca gttctccttg tacaactagt cgcgtgacgg cgtccatgtc gccacaagta
    63361 attagtgtga caatttcttt tccctcgact tcattcagta attgggtatc tgtaggagca
    63421 actttttctt taaaaaatgt tttgtatgta tacacatttt ctaaatctgt caaatagatc
    63481 ttggcaccca tctctaaatt ttctagcggt gtaaacagca attcagggtt cgttgcacga
    63541 tggcttgcta aggcataatt tccttttccc atttcttgat ctggagataa tgttcctgct
    63601 ccgtaaagta gtgacgtatt atccaagcct ttgaaaaccg gtaaattgat tgaaactgaa
    63661 gggactgcga tactagcaat cactggtaga tctgttcctc tcatttgcga cctcatgacc
    63721 ccttctgtcg tcattggttc taccgcatca aagtcaaaac tgacatcttt atcgttattc
    63781 tttttcagat cttcttttgt tacattcgct atagcatatt tatctcctgt ctctctgaca
    63841 aaataatcct tgatctgttc attgaaaatc aaagctagtc ctactaataa caagaggaac
    63901 aagaggatat tgactagcca tctttttgct tttccttttt ttttcatttt ctctccctcc
    63961 agactcattt tactgttttt cttttgctct ttccaacagc cgaatgacac atttctacaa
    64021 ctctgtgtat ttttttcgta cacctgcgct tttttctaca ggatatttca aattattttt
    64081 gatcaatttt tcttcaatga tctcatctag atccaggtga aggttgtctg ccatcatgta
    64141 tgaatagatc aatacatctg ccagttcttc tttgattcgt tctaattgtg tttcttttac
    64201 ttcttccggc gttttccatt gaaaaagttc taacaattca gatgcttcca aagaaataga
    64261 gatagccaaa tctttttcgt tatgaaattg tcgccaatta cgttcatctc taaactggtt
    64321 gactttttcc atactattca tttgcttgcc ttcttccaat cacttcttac ttgattaatc
    64381 ataccatttt tttacataaa aaatagatga tgcttttaaa aaaactcctg ttttctttta
    64441 tttaaaataa atgtcataga aaatcaataa tttaggtagc caccatgtgt atgcagtagc
    64501 gtgtcttctt cccttgaggc tttttgtttt tcgatacgtt cttcttccca aacagacaac
    64561 gctactagaa tctctcttaa tgattttcct tcttcggtga gcgagtactc tactttaggc
    64621 gggacttggt tataggcttt tcgatggata ctttcatctt gttctaactc tcttaactgc
    64681 tgggtcagca ttttttttgt gatcgtaggg atttgcttgc ttctccacgc ctaaaataag
    64741 cgatattgct cttcccttta tctggtgtaa gcaaagcagt tagcattttg acgatcgtgg
    64801 gttccgcttt tcacttctta tctacttagt atcatgaagt atactttcgt actaatacta
    64861 agtagcggaa gtgtcaacta ctttgtttaa ctacatagca aaagaaacag aagggtgaca
    64921 acggtcatca cgcttctgtt tcttttgaat atgctgtgtt gttgtctcta tctccgtgta
    64981 gactttcctc aaacttatcc tttgacagta agtcagtagg ctgatttgct ctttctagat
    65041 gccagataca tttctacaaa aaaatagtga ttctgttttg aaaattcgat ttttagttct
    65101 gcattttcgt aaacatccaa catttttttc gcactgtata gacctaaccc gccatttttt
    65161 ttagaggttt ttcctctttt gaccatctcc gatagcgtca aattcgttgg atttgccttg
    65221 ttcgctatac taaaacgtga tccctctttt tcttccagca gattgatcgt aatcggactc
    65281 tgatcggcta cacgatgatc gaatgcgtta gaaataaaaa tagataaaca gcgaataaaa
    65341 acaaccagat caatcgggat tgttttcact tcgacaggaa taactacttg gatcgggatt
    65401 ttctccgatt ctgctttttc tagaaatttt aaaaagacac ccctgacagc tggggataca
    65461 atatttgtta gctgttgtat ttgcggtttt tcaaaaatag ctgcagaatg atcttctaat
    65521 atttctaaaa attccaacgt ttctttttta ttttcctgaa gagtaatttt taatgaaaac
    65581 agtagacttt tataatcatg tttgaattcc tctagatgat ggtaatattc ttcatttatt
    65641 tgatacagtt gaagcgattc ctgatgttgt ctttctttta cttggaaact gatcaacata
    65701 atggaaaaaa acaaagaaaa aaaactgatg actatcgaca aaagagatag aagcagataa
    65761 ttagatgagc ggaagtgaac acttcccaat accaagtaaa tcacgcccca gcttaataaa
    65821 atacaaatac ctccaaaata gtgttcagtt ggataagtaa aaagcaagtt gagaaatctc
    65881 gtctgtttgt ctatactttt tactaataga tagaaaataa atgcgataag tagtgcaaaa
    65941 aaacttgctg ttccaagatg tctctttcct aaataatcca tcagatcaaa aaccagaaaa
    66001 aagctgagtc cagtgatagc aaaaaaaaga ttactgatca aacagctgat aaacaggttt
    66061 ttcgtagtaa aataaaaaag aaacatcaat aataagccaa ctaggtaagg aaagctcact
    66121 ttagggatag gaagtaaaag tgtcaccacc atcaaagcta tacaaccaaa gctgtttttt
    66181 actagcagtt gtttcgaaaa gaataaatag atacctaaat ctaaaatcaa agtaaaggtc
    66241 aaataaagtg tcactgttgc gaccatctta ttcctccatt gttttgtttc aaaatgtaca
    66301 catatttttt taataggagc ctgcatatga aaaagtattt tatcatagaa gataaccagt
    66361 ttcacagcga atggatcatc agagaattgg tcaatcatca tatcgagaat atcgtgcagc
    66421 tcacttctat ctctcaaatc aatcagttga tccagttggg cactattgat aatcacgatg
    66481 ttttctttat cgatatcgac ttgaagcaat cactcacagg tattgatgtc gccgaacaaa
    66541 tacgtaaaaa aaatgcacat agtaatattg tttttttcac cgcttatacg aattttgcag
    66601 tagagatcat caatcggaaa atccttccga ttggatatat cagaaaagac aggaatttat
    66661 ctgaacaaat tcgtcaagtg ctagaacaga tcaattggaa agaacagcag atcgtaaatg
    66721 tcgataaaat ctttattgat caaaaaggtg gaagtctcgt cctgtatcct cgggaaattt
    66781 tatatattga aagtatcaaa ggtttgaaaa accaagttat ggtcaaaaca actacagaag
    66841 ataaattagt gaatacttct ttaaaacaat tgaagacaga gttagaaaag tatgcttact
    66901 ttttactttt aaaatcttac tgtatcaact tatcgcaatt gcagaaaatt gattctgctc
    66961 attatgtgtt agaatttttc aacggtgact ccttactcgt tggtcgaaag atctttgaaa
    67021 agacaaagga acgtttccat gacttccaaa aaactgcttc gagttgatga ataagagctg
    67081 agacgtaagc atagcagatt tcagggagtg ggccttgaat cggctccact ccctgaaatc
    67141 gttgatatga ttttacgctt cctcttctgt aggagcatct gttaacgacc atgtgaatgt
    67201 tgaaacgtag ttgtctacag cataattgga tacaggaaca aataattcca ctgattgtct
    67261 cccttgttcc acattctctc ctaatgaata aatccatgtg ccaatccctt catttgctcc
    67321 tgcagttact agatctactg cctgatctgc tgggatttcg attccttcag cgccagttcc
    67381 agaaacggta gatggcattc cactgctatt agttgcgcta tttcgaatca cggcgttttt
    67441 gaatttcacc tttgaaccag ttagtttatt cgtatcagat gttgtaccaa taaattctgt
    67501 tcgtttgact gataatttcc aaccggcata atttccgctt ttatccgtta cttgtacgaa
    67561 attctgtact tgtgtttgtg ctccagtact gtccgttccg ctttgtaatg cagcatatac
    67621 tgtttttgct gtacctaact tgattgttcc aaaatcaaga tctggagctt ggtcaatcgt
    67681 cagtaatccg gatgttccct gatttggttc gtcaggtgta aaaggagtat caggatcatt
    67741 tggatcaaca ggaggaactg gaacaacgtc ttcaggattt gcagcatctg gtttggtcac
    67801 agaaatcgtt ccttctgtag tggctgagct cttattctca gcaaaaacac tttgtgtaca
    67861 taataataga cttccagcta gaacggtact taatgcaatt gtttttttca tttctttttc
    67921 cttctttctt ttaaaataaa taatccagca aataagccaa taagaatgac tccaacaagt
    67981 atcagccatc gccagtcaaa tgtttctggc tgatttttta aatcaatatc cttttcattc
    68041 aattggtccg ctgcatcatc agttatttca aaatcggtat ggagattcca gcgtttttct
    68101 ttccaagcac ccttcacaac cgcttgataa tgtcccggag ctaatttttt tccttctaat
    68161 gggacaagta aatcaaaatt tgtattgggt gctacatcta ttttttcttt ttttattgaa
    68221 aggtaaggct cttttgtttc ttttttgtag atttcaaccg atgtagtcaa gccgaacata
    68281 taagaagcat ttggattttg aagattcgcc ttaatcacat ttcttccatc gctttgatcc
    68341 gcataagctt tttttaattt caaatctggg gaagactctt tttcgctatt tcaaatcaag
    68401 attgctttca cttgagaaaa ctgattgacg acaccaactg tacttttgtt agaaacgttc
    68461 tctttttttt cttgaaacgt gatccctcct gcgaccacac cagccagctc tttttctggc
    68521 gctttcagtg aaagtgtctc agtgaccgca ctttgaggag gaacagttat ttctcccact
    68581 gtttttgcta agtttttcat atcataactt aatgtttcat caaatttact ttttgattct
    68641 ccatattcga tcacaccgtt tgaattcgtc gtagcggagt ggatcaatgt ctcaactgtt
    68701 actgatttat ctgttcggtt aaagaccgtg taggggattt cctgtgtttt cccaggctcc
    68761 atccttacat ttatatatgt tttgttctta tcaatctgat tagccggtaa ttcaaaatct
    68821 accgcaaaat tgaattcgga ggcatggaca ggtgtctgcc aaaggatgaa tccaaggaga
    68881 aggatcataa gtaaagagca gttgtttcga tgacttttat tccatattcg attcatcaat
    68941 catcctcctt gatctttatt ctgtcggcgc atctgctaat gtccatgtta tctctgctga
    69001 gtaattacca gctatctgat tttctttggt taagacaagt ttaaacgccc gaccgttatt
    69061 taaatattct gaataatctg cttgcacatt cgttgaattt tgtcctctga caatttccag
    69121 ttcttcttca ttcactaatt gatactcctc tcctttcttg aaatatagga catcctgcat
    69181 agaaaatcct gcatcatttg tgaaaggctg agataatttt atcgttaaga tccacggatt
    69241 tttcgtttct cgctgatcat aaatccataa tttttcctgt atatccattt ggccaaatgc
    69301 ttcttgtgtc aaaccagttg ctttgatctg accaaaatcg agtttggaag gggcaagaaa
    69361 tgagagacca ccttctacta cgatcttcgt agcaggtgca aaaggcgggg catcatgata
    69421 aacaactggc gtatcacttg gattgtgatt cccaatttca tcgttggtac ttggcttgtt
    69481 ctgcacaccc ttagcagtag cttctcctgc ttggtcgttt aatacaattt ctaatacatc
    69541 tcctgcaaaa agttgatttt ctgggataga aagaacaaat tcccctgtat catcaatagt
    69601 tgtttctgac aaaacagctt gatttttttt caatgaaacc aaggctcctg cttcacctgt
    69661 acctgtgatc gtgcgagaag ctgcattgat caatgtggta cttaaattag caggttcagg
    69721 aggaatcaca tcgactgtcg ttttggcaga gtccagttct gtcacttctt ggccacctct
    69781 tactttttta gcagcaacaa cttgcacttg atcctccgtt tttaatctct catcattggg
    69841 tacttgaact tcgaacaagc cgccttgttc gccttctccc catgggttat atcctgcccc
    69901 ttcattttca tctgtttgtc cttgcgtgag agcagtaatt tctacagatg atttcgtcgt
    69961 atctgcgtaa gtaatacgta aagtcacttc tacttcgcct gtccaagcat ctcttggctt
    70021 tgcctcgaca ccttcgggaa caacgacatg cccatagatt tttttatctg cattcgttgg
    70081 cactcttaag gattcaatca tcgggagctg attattggca ctgattcgat tataattttt
    70141 cattccggca cctgtctgag caaattcatt tgttaaacct gcactagggc ttgatacgaa
    70201 attgcctaag tcggtcccat tgatcgataa gtctgtcata tcagaaaaat aatccgggtc
    70261 tttttcaaag ttatcgaatg ttgcgcctgt ttttcgccat aaagcgattt ctgaatgggc
    70321 gatctttaaa gaagaatctc catttccttg gaatacccag ccgcctatgc cattggtctc
    70381 gcttccaatc cgtttgtttt ggaaatcaaa ataggtagga gattctaagt ccacattcaa
    70441 cacatcccca ctgatgatgc cggtcttatc ttcccctgtt ttcccgacca tgactaaata
    70501 agttttttca cctgccgtaa aatttaaatc aatatctcca gttgtatcta cagttgctcc
    70561 ggaatctgcg cgtatatcga tatttgattt ttctccagtg actgtaaaat cagctgaacc
    70621 tggactttct agaaaaccag aagcgtcata aaattggatc gcttgatttc cttgattgag
    70681 tctgttcacg taagatttgc catcacctgt atttcttacc ctcaatgagc ctccattggt
    70741 tactgtcagc ttttgcgtac cattctcaaa acgaactgcg ctggaacgcc cactctgctt
    70801 atcgatgtcg ataactgatc cggctccttc tacgatcaaa gaaaggcctt ttcctacaaa
    70861 acgcatccca ttgttcccgg gttgatcgtc atctccagcc attttgaccg agatttgtcc
    70921 accacttgat acatggattt gactagaatc tccttgcaag cgaaatccgc ccgtagttgc
    70981 tgtattgatg gtgattttcc caccattcga tacagtgact ttcgatcctg aacctctcat
    71041 ttggatgatt ccgcgattgt tgtcattggt aatattgatc tttgtattta ccgaaaattc
    71101 gcttcccgaa ccactgactt caaacatgac attaccaggt agcgtatcga ctgcctctcc
    71161 ttcatcgcta acgccatctg cccgatcata atcaagagag acctttgaac cgcttaagat
    71221 atggattcct ggcgctgttt ttccactacc agtctcatct cctgaatcta gaaaaccgta
    71281 gaatttccct agatacggtc cataaagttg actaccattg atcacattca attgatagct
    71341 tctacaaaaa taggctttac ccggagcggc attcagtcct tccttcactg gttggacatc
    71401 caacacaccg gaaacaccat ctaatgtgac agttgcacga ggagcgtaaa tcagtcctaa
    71461 tttggaccca tcatacaaat caagtgtccc ggtaaatttt atttctcccg taaaatgata
    71521 tctgcttccc caaaaatcgg tcatataagc aatgataatg gcatttccat cgatatttgg
    71581 tgtcaatcca cctggatgtg cagctgttcc tttaaaattc tggaactcga tcgactgagg
    71641 tatctcattg atataaatat ggtgtgtgcc gaaattgtga gcgaatccac caaaatcaat
    71701 aacgatatct cgtttgatct ctaattccct gtctaaagtc aaaggctgaa tcagtcggat
    71761 cttagttact gattcgtctg cgagaagctg cgccagttct tctgttgtgg cagcttcgct
    71821 gtacccatcc gctgaaacag ctcttgccag tgccgcttca ctgctatcag acgtttgttc
    71881 agctacttct gaggtctcac ttgtcttatt ttcttcttgc cttttattag tagaagcctc
    71941 actttgagat tctgtagttt cagaagtaat cgattcttct gaaaaagtag tcttgtcaaa
    72001 actttgattc tctgattgcg ttgattcgtt tgttttctct acatcggcaa aaacggatgt
    72061 caccatcaag agagggaaga caaacaaaat cgacgcaatc agctttttta cacattgatt
    72121 cattctccac catcctctct gacgttttga caattttatt ttatcgtttt aatctatttc
    72181 caattgtact ttcatattta aaatatgtat ttttatggaa aaatttttct attaggtgta
    72241 aatttctttt ttgtacaaaa aaaaggctgt gacaaaagtc ttgtcacagt cccttatccc
    72301 gaacaagcgg tgttctcccg ccaactcctc ggtcacaacc tccattttaa caataaacct
    72361 gtaaatgtga gggcaatact ttgattgtca caggtaattt atccccttca tctccatcaa
    72421 cattcgtcgt caattcttcc tcatcagcta atgacacaac taattgctca caagtaaggt
    72481 aggctagatt tttcgtagat tcatctactc cttttaatag gtctggcaat gttttaagcg
    72541 tatcgatcaa agaagaatct tttaaataaa ccaaatggag cttaccatca tccacttttg
    72601 catttggaag taatgtttca aaaccgccga ctgagttagt tagtcctatc agcaaggtac
    72661 tgctctcaat ttcttccttc tttccatcaa cttctacgtg aaacgagtag gattgtgtgg
    72721 aagctaactg tttgattcct gacatgaaat aagctagttt tccaaatttc gtcttcttct
    72781 ccgcatctac atcattgatt gattcaggga tagagccaat tgcgacgaca ttcataaaat
    72841 agtcgtcatt tatttttcca atatccaatg gcttgactga ttcaatcgaa aaatgattga
    72901 tcgcttcctc aggttctagc ggtatcccta atgctcgagc caagtcattc accgtaccca
    72961 atgggaaaaa tccgaaattt ggccgatgtt cttgttcagc gattccactg attccttcgt
    73021 tgactgttcc atctccgccc atgacaaaca cactatgata cccttccgta gccgcttcgc
    73081 gagtaaagtt ttttgcatcc cctgcttttt ttgtatgcaa gacgaccact tcatcaaaca
    73141 ccgattccaa ttttgctatc gctaattgct caaattcctt tgcttgttca ccgcctgaac
    73201 tggggtttac cactaataaa acttttttca tcactttctt tcccttctga ttgatttctt
    73261 taatataacg atagcataaa gaaaaaaatt aggctgtgat gaagtactgt tcatgaaaca
    73321 ttcatttatt ttttgacaag ataaaacatc catactatga aactgaggaa aattaaaaca
    73381 atgatatagg aaataaaaga aaatgatagg cattgtatac ctaataaagt aaaaatctta
    73441 ggtggcagat aaaaacacaa aaatagcaag agaatgtaat agcgtttctt ccactttttg
    73501 ttatcagacc ttatagcgat cagaggaata ccaaatgcat aactgaaaaa ctcttgatgt
    73561 actgttaata tgtcacggcc ttgaataata aatgcgacaa tccccgctat aattagtgtg
    73621 cctaacacca aaacggaaaa ttgatgaata tattttatat acaaatgatc cattgtactt
    73681 cctgcctttt tatttgtgag aaaaacaaat ccgtatacgt gaataacttc tagaaataaa
    73741 agaaatttgc aaaaccactc catttatcta cttctacgtg cattttttga tattgttttc
    73801 tttatttaag attatacaga aaaatatcta gggtcgctgg attctaagta aaaaaaattt
    73861 ttaaaataaa aagaactaaa tcaagtagta attaaactac tgttgattta gttctaataa
    73921 aaagatttag atgaacagtt ttattgattt aaatagttca tattttttat ttataccgct
    73981 tatttactac ttgctgtact agaatctgaa gtgaatgtac tcaaaacatt ttcaaagtct
    74041 ttgtctttga tttttacatt agcgtctttc aatacttcgc caattacttt attctggaag
    74101 gtactgtcac ttaattttgt gtctgttgca atttctttca attctttttt gtatttatcc
    74161 atgtcgttgc ctttgttttg gtttttcacc attttaacca cataatattc cgtagtataa
    74221 gttgaagcat tggtagaagt gatcacatca gaaacttgac catctttcaa cttaaaggct
    74281 gcttctttga cttctgccgg aaccgttgta gtagtggaat caaatttcac ttttccgcca
    74341 tcttcttttg tcgtagtatc agttgacttg tctttggcta atttagagaa atcgtctccc
    74401 ttatcagcag ctttcttcac atcttttgca tcatcttcac ttgctacttg gataatttga
    74461 gcttccactt ctggatggaa agattcccaa gccgttttta aatcttcatc ggtcaatttg
    74521 atatgtttct ttagaccttc ttgaaaggct agactttgtt tgatactgtc tttgaatgtt
    74581 tgttctgtgt aacctgcaga tttcaattgt gaatcaaatg aatcacccaa ttgtttcttt
    74641 gtttgatcat attgagaatc gatttctttg gttgatacgt catctccata tttatcttca
    74701 aatacttgat aaacgatcaa ctgttgcaca gcggaatctg ctccttgcat ttttttcaag
    74761 ctatccatta cttcttgttc gctgattgta ccgcctttat aactaacggc tgttttttca
    74821 ccggcattcg tacagcctga caataagcca gtgcctagta cgatggcaag tgctaaaaat
    74881 ctttttttca tttatcaatt ctcctacttt tcgttttttg cgaattgcta aaaacaaagg
    74941 tgttcaagat gaaaatcatc aagaacacct ttactaatct gctcgatacg tcctgaatag
    75001 atataaggct acacaatcca gaaagttcgc tcagcagaat aaatgtcaag tagcacatca
    75061 caccttaata tactacttct tttgttaccg tttttcaagt ctatcttgcg ttccgtattt
    75121 aacgtaacta attcttagaa caaatgttat cgattttgtt tttcttcttc cgctgctggg
    75181 atattcaaat cagacacttt tatgttcgat tttccaga
//
LOCUS       DACCXW010000002        59635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCXW010000002 DACCXW010000000
VERSION     DACCXW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.002"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            149..417
                     /locus_tag="IVY71_000069"
                     /pseudo
     CDS             149..417
                     /locus_tag="IVY71_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            1089..3487
                     /locus_tag="IVY71_000070"
                     /pseudo
     CDS             1089..3487
                     /locus_tag="IVY71_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266211.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3970..4209
                     /locus_tag="IVY71_000071"
     CDS             3970..4209
                     /locus_tag="IVY71_000071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7984150.1"
                     /translation="MDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNK
                     HLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(4633..4803)
                     /locus_tag="IVY71_000072"
     CDS             complement(4633..4803)
                     /locus_tag="IVY71_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7984151.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(4997..5176)
                     /locus_tag="IVY71_000073"
     CDS             complement(4997..5176)
                     /locus_tag="IVY71_000073"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984152.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            5563..6675
                     /locus_tag="IVY71_000074"
     CDS             5563..6675
                     /locus_tag="IVY71_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7984153.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(6753..7046)
                     /locus_tag="IVY71_000075"
     CDS             complement(6753..7046)
                     /locus_tag="IVY71_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7984154.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            7214..7528
                     /locus_tag="IVY71_000076"
     CDS             7214..7528
                     /locus_tag="IVY71_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7984155.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7549..9528
                     /locus_tag="IVY71_000077"
     CDS             7549..9528
                     /locus_tag="IVY71_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7984156.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            9541..9867
                     /locus_tag="IVY71_000078"
     CDS             9541..9867
                     /locus_tag="IVY71_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7984157.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            9883..10401
                     /locus_tag="IVY71_000079"
     CDS             9883..10401
                     /locus_tag="IVY71_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984158.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            10401..11753
                     /gene="celB"
                     /locus_tag="IVY71_000080"
     CDS             10401..11753
                     /gene="celB"
                     /locus_tag="IVY71_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7984159.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(11837..13342)
                     /locus_tag="IVY71_000081"
     CDS             complement(11837..13342)
                     /locus_tag="IVY71_000081"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7984160.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(13434..14024)
                     /locus_tag="IVY71_000082"
     CDS             complement(13434..14024)
                     /locus_tag="IVY71_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7984161.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            14203..15849
                     /locus_tag="IVY71_000083"
     CDS             14203..15849
                     /locus_tag="IVY71_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7984162.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            15898..16617
                     /locus_tag="IVY71_000084"
     CDS             15898..16617
                     /locus_tag="IVY71_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7984163.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            17096..18043
                     /locus_tag="IVY71_000085"
     CDS             17096..18043
                     /locus_tag="IVY71_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7984164.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            18055..18975
                     /locus_tag="IVY71_000086"
     CDS             18055..18975
                     /locus_tag="IVY71_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7984165.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            19016..20473
                     /locus_tag="IVY71_000087"
     CDS             19016..20473
                     /locus_tag="IVY71_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7984166.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            20818..21432
                     /locus_tag="IVY71_000088"
     CDS             20818..21432
                     /locus_tag="IVY71_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7984167.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            21425..23149
                     /locus_tag="IVY71_000089"
     CDS             21425..23149
                     /locus_tag="IVY71_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7984168.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            23146..24597
                     /locus_tag="IVY71_000090"
     CDS             23146..24597
                     /locus_tag="IVY71_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7984169.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(25070..27223)
                     /locus_tag="IVY71_000091"
     CDS             complement(25070..27223)
                     /locus_tag="IVY71_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7984170.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(27225..28271)
                     /locus_tag="IVY71_000092"
     CDS             complement(27225..28271)
                     /locus_tag="IVY71_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7984171.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            28421..29710
                     /locus_tag="IVY71_000093"
     CDS             28421..29710
                     /locus_tag="IVY71_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7984172.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            29723..32425
                     /locus_tag="IVY71_000094"
     CDS             29723..32425
                     /locus_tag="IVY71_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7984173.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            32410..33267
                     /locus_tag="IVY71_000095"
     CDS             32410..33267
                     /locus_tag="IVY71_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7984174.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(33338..34195)
                     /locus_tag="IVY71_000096"
     CDS             complement(33338..34195)
                     /locus_tag="IVY71_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7984175.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            34406..36577
                     /locus_tag="IVY71_000097"
     CDS             34406..36577
                     /locus_tag="IVY71_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7984176.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            36645..36986
                     /locus_tag="IVY71_000098"
     CDS             36645..36986
                     /locus_tag="IVY71_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7984177.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(37262..39016)
                     /locus_tag="IVY71_000099"
     CDS             complement(37262..39016)
                     /locus_tag="IVY71_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984178.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(39027..39863)
                     /locus_tag="IVY71_000100"
     CDS             complement(39027..39863)
                     /locus_tag="IVY71_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7984179.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(39865..40548)
                     /locus_tag="IVY71_000101"
     CDS             complement(39865..40548)
                     /locus_tag="IVY71_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7984180.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(40707..42101)
                     /gene="uxaC"
                     /locus_tag="IVY71_000102"
     CDS             complement(40707..42101)
                     /gene="uxaC"
                     /locus_tag="IVY71_000102"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7984181.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(42140..43639)
                     /locus_tag="IVY71_000103"
     CDS             complement(42140..43639)
                     /locus_tag="IVY71_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7984182.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(43632..45053)
                     /locus_tag="IVY71_000104"
     CDS             complement(43632..45053)
                     /locus_tag="IVY71_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7984183.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(45113..46105)
                     /locus_tag="IVY71_000105"
     CDS             complement(45113..46105)
                     /locus_tag="IVY71_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7984184.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            46337..48535
                     /locus_tag="IVY71_000106"
     CDS             46337..48535
                     /locus_tag="IVY71_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7984185.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(48993..49865)
                     /locus_tag="IVY71_000107"
     CDS             complement(48993..49865)
                     /locus_tag="IVY71_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7984186.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            50165..51139
                     /locus_tag="IVY71_000108"
     CDS             50165..51139
                     /locus_tag="IVY71_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7984187.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            51161..51853
                     /locus_tag="IVY71_000109"
     CDS             51161..51853
                     /locus_tag="IVY71_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7984188.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            51819..53327
                     /locus_tag="IVY71_000110"
     CDS             51819..53327
                     /locus_tag="IVY71_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7984189.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            53401..54504
                     /locus_tag="IVY71_000111"
     CDS             53401..54504
                     /locus_tag="IVY71_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7984190.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            54507..55844
                     /locus_tag="IVY71_000112"
     CDS             54507..55844
                     /locus_tag="IVY71_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7984191.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            55831..56709
                     /locus_tag="IVY71_000113"
     CDS             55831..56709
                     /locus_tag="IVY71_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7984192.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            56706..57629
                     /locus_tag="IVY71_000114"
     CDS             56706..57629
                     /locus_tag="IVY71_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP7984193.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            57626..58036
                     /locus_tag="IVY71_000115"
     CDS             57626..58036
                     /locus_tag="IVY71_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7984194.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            58040..59608
                     /locus_tag="IVY71_000116"
     CDS             58040..59608
                     /locus_tag="IVY71_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7984195.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT        19243 a        10453 c        12261 g        17678 t
ORIGIN      
        1 tttatggata gtaattttag aggggaatca atgaaggaag aatagaaggg aaaaatagtt
       61 gcaaatacgc cagttagcaa aaatagcaat acatcttcaa gctttaaaat tcgagagaat
      121 catcttggta gtggaatcaa ctacaacaaa aggattaaac caattaatag atagaattac
      181 caatggaaaa atagaaaagg tagttattct atacaaaagt tgtttaattc gcttcggtta
      241 tgaacttagt gaaacccttt gcaatatatg gtacagaata tagaaattat tgacattaca
      301 gaaaatgatg aagatttaat acagattgtc actatttgta gatacctttt acaaggaaac
      361 cgagccaata aagctaagaa gatgattaag gagttgatgg aagacgatac aagttaagaa
      421 agtgaaacta cgcccaaata aagagcaaaa agagtaattg gacgtggaaa atgcaagaaa
      481 tgaaccatcg attcggtgga cagtttatta gtaatgacgg tttgagaaca tccatgacga
      541 gaatgaagga acatcctaaa tgcgtttagt tgaaccaagt gccaaataat atggccaaat
      601 aagcgatcag ttatcgatga atcccaagta ttctaaacta tatattcatt ttgatgctaa
      661 gcattgatat atttttttgg gattgatgaa ggttaaccaa tacagggact cactgatgaa
      721 attctagagt ctgaccttgg tatcaacgat ttagtgattg tttctaatgg aaaagtataa
      781 aagtatataa gtacattaac aaaataacta ttagggttac gctccagctt gtgcaaggca
      841 ctggcaggag gaatcgtgaa atcataatta tggttaagcc acacggacaa ctaaaagtca
      901 tacaaagcta tcgaatcaat caacagaaga caatcggcaa tcattagctt tgtttacagc
      961 ttacattgca acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag ccattttgag
     1021 ttggggaatc tttttagaaa attatcgggt accttgttgt ttagtcaaaa ttataagggg
     1081 gagtaatagt gaggaacaaa tttcataaac tgttaagtat gtttctagtt atgcttattg
     1141 tgatcaatca atttgggaac ataacggttc tagctgagtc tttagcagaa cagccagagc
     1201 gttcgctgac gatcgaagtt gatcaagagg aggctgcttt gggtgagcag ttccagttgc
     1261 gagtcagagg aacaccagac ctgctaaatg cattggtcat tgataagagt gaggatttga
     1321 ttgaagggga agtgaagcaa ctcaatgatt acgaacgtct gattacttat tcttcccagc
     1381 attcaggtaa atacgaattg accgcaagaa gcggtgatat tcaatcaaat acggtctcaa
     1441 tagaaatcag tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca gaagaaagca
     1501 caagtgaatc ttttaatata ccagaagaaa gcttaagtga atcttttaat gaagccagat
     1561 catctgagcc atctataacg caggaatctc agattgaaag acaagaggag ctcttgaatt
     1621 atgacatcgg catctctgct gagcgcccca ctttgccgag caggcataca caaacgttaa
     1681 agggatacat tcaaggaggc gctggcgata ttcttgaaga atatggggag atggtcgtac
     1741 gagttcctag agcatcgatt ggcttaattg agttcattag agtggaggtc gctgatgatt
     1801 cacctttaaa gccactggca ttttaccaag aaggcacaga cgttgttttt ccatttacct
     1861 ttgattatga aggattaaat gaagccgctc agtcgtatac attcagtatc acgtttgaag
     1921 ttgctttctt tcaatcaggc ggtactgagc caaataatga ctttcaagtg agtctactta
     1981 tgctgagcga gcagcaaaca cggaattttc aattacgcct attccttcat caagacccat
     2041 tttttcgaaa ttatactatg gctctagaga aatcgacagc caaggaaatc ttgggaatct
     2101 gctgattgac gggcaagacg tgaccaacag taataaattt attttgcagg taaattatga
     2161 aggatcacga aaagacggtg tagtggttac cgatgtacta ccgcaaggaa caactttggc
     2221 accgaatccg actacatgga gttccactgg aaatagagaa cctatttcag gtggggttcg
     2281 gatcgcagaa gtaacgaatc caggtgaagc aaacgttaga actcattatg tttccgagga
     2341 atttgtggac aaagtatctt ttaataagga aacgaataca cttgttgtgg actttggtaa
     2401 tctagcagag ggtcaatcgt attttattga atatgctctt gaagtgaccg atgtcgactt
     2461 gttcaatgaa ggaagacaag aggatgggtt ttctcggaat cgagcgagta tgacctacaa
     2521 taatcaggta atgagccgag actaccgctt gagaatccct gaaagtaatc ttccggcaat
     2581 ctctttaaga aaaagtgtgg acaaaaatgt tatcaatctc aatgaaaatg aactaacgta
     2641 tactctgcat gtctcgatgt tttctggaac aagaaatacg cttgttattg aagatgtttt
     2701 ggaagaaggc atggaatttg tgtcatttct atcagatatt ccctctttca ttgatgccga
     2761 agtcaataat ggaatagttc gtttcagtgc gaataatacg attggtggtc cgacaagttt
     2821 taccctctct tttaaggtca atgtagaggc gtatgcggtc ggcgatcaaa ttttaaactt
     2881 tgcaacgctt gtgaatagtg acaatcgatt gaatacgagt actgtaagaa caaggaaaat
     2941 cgatgggcgt attaaagtaa ttaaaactga tgagaatggc caaaggctgg caggagcaaa
     3001 atttgaaatt ttatcaagcg attcacaagt gatacaagaa ggtataacgg cagaaaacgg
     3061 agaattttta agtgaaccgc ttcctgtagg tagctatcaa gtccgagaaa cagaagcacc
     3121 ggaagggtat cttttagatg cgacggtaca tgatgttttt ataaatgaat actccataga
     3181 agaagtgacg ttaaccattg aaaacatcct tgatacgggt ggtgttgaac taattaaaca
     3241 tgctgaaggc agtggtgaag tccttcaagg agctgttttt gagttacaga accgagaagg
     3301 agaaacgcta caaaccggtt taactacagg agaagatgga aaactggcaa ttgacggttt
     3361 agcaccagga gcatatcagc tggtagaaac tcaagcgcca atagggtatg aattagatgc
     3421 gacaccaatc gagtttgaga tcgaaagaag tcagacagcg gtggtagaat tgacgaaaga
     3481 aaaccgactt acaccaggcg gtgtcgtgtt gacaaagatc gatgaccaaa gcggggaaat
     3541 cctgcaagga gctgtttttg agttacagaa ccgagaagga gaaacgctac aaaccggtct
     3601 aactacagga gaagatggaa aactggcaat tgacggttta gcaccaggag catatcagct
     3661 ggtagaaact caagcgccaa tagggtatga attagatgcg acaccaatcg agtttgagat
     3721 cgaaagaagt cagacagcgg tggtagaatt aacgaaagaa aaccgactta caccaggcgg
     3781 tgtcgtgttg acaaagatcg atgaccaaag cggggaaatc ctgcaggagc tgtttttgag
     3841 ttacagaacc gagaaggaga aacgctacaa accggtctaa ctacaggaga agatggaaaa
     3901 ctggcaattg acggtttagc accaggagca tatcagctgg tagaaactca agcgccaata
     3961 gggtatgaat tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg
     4021 atctttttaa caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat
     4081 ccaacaagca gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt
     4141 atattgtcaa tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga
     4201 aatatatgag ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca
     4261 tcaattgaat ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag
     4321 tagagaaggt tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag
     4381 tgaaaaataa acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa
     4441 atcattcttg agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt
     4501 tgattttctt tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc
     4561 tctttgtcaa taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact
     4621 cgtgaataaa ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac
     4681 caatttcgca tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt
     4741 aactaagaat tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg
     4801 aattttttgt gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata
     4861 aaactcagtt tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag
     4921 acagaattct tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa
     4981 cggttgtcaa agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca
     5041 gtaaagaagt gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat
     5101 aactttgagc gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc
     5161 gtataaccat tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt
     5221 ttttcaccac cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga
     5281 cacatggagc agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt
     5341 cgtaagagac ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag
     5401 aaccattcat aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca
     5461 agaaaacgat atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga
     5521 caacgccata tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa
     5581 agtaaaaaga aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt
     5641 attttaaaac aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt
     5701 tcattgtttt tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt
     5761 acaacattat ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg
     5821 agtattcgtt ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc
     5881 gtcatgttct cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc
     5941 atgttgattc attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa
     6001 atggggaatg cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa
     6061 gattcgttga acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt
     6121 tggttttcag ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt
     6181 aaagagattt ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca
     6241 tttggcatta gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg
     6301 gcagggtttg cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa
     6361 gaacgagcag tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc
     6421 agccaattta ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt
     6481 tctacctata gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt
     6541 ttattcgggg ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt
     6601 ttaggaatca tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac
     6661 ggtttgaatc attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga
     6721 tgataaattg ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact
     6781 ttcattttta atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg
     6841 tgatgctttt cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga
     6901 tcttctagtg gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg
     6961 acaatgtttc tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta
     7021 tacaaaatat tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta
     7081 tatcacggtg ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg
     7141 gaaatatata ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag
     7201 gagtgattga tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat
     7261 gatggcctca aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc
     7321 agtttcagct acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat
     7381 cagcccgcaa acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa
     7441 gcctgttgtt agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt
     7501 agcagaggtg atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag
     7561 gaaaaattgc tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt
     7621 ttagcttctg aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa
     7681 gagttagtag aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg
     7741 catattttac aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga
     7801 tatgctagag aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac
     7861 caattgcttt tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc
     7921 agtcgttcca gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca
     7981 ctaaaaatcg tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga
     8041 cggcatttta tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat
     8101 ttagggcaaa gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc
     8161 gatgaaacaa gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta
     8221 cacttggctt tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc
     8281 gaacaagaag tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt
     8341 gaggtaatcg caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc
     8401 atggctaagt cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg
     8461 aaagttctga aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt
     8521 ttcaatggac tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc
     8581 attgagaacc cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta
     8641 aaaaggcatt tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg
     8701 gcctatttgg ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta
     8761 catgcattga ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta
     8821 gtcagtgaat tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat
     8881 gaaacaactt taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt
     8941 ttcaaaattc cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata
     9001 cgaaaggtag ttggagaatc tataccctat cattcctcag atagaccgat accagcattt
     9061 tctctacagc aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact
     9121 tatacgtacg aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata
     9181 aatgaagaag aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg
     9241 ggacaaatca tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc
     9301 gaaacaaagg tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa
     9361 attcattttg tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg
     9421 actaaagcaa tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa
     9481 cctatttttg agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag
     9541 atggctgaaa aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat
     9601 agcggaaatg cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt
     9661 gaagaagtag aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca
     9721 caaactcaac ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg
     9781 gtccatgcac aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg
     9841 aattatttgt atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa
     9901 aggaaaatcg aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat
     9961 ctaatcgtac gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg
    10021 cttctatcag ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg
    10081 cctgcagtag gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca
    10141 aagtggtatt ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc
    10201 tcaggattta cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt
    10261 ttcattcttg ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa
    10321 atttcactca accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat
    10381 ttaggaaagg agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg
    10441 ggaaaattgc gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc
    10501 ctttcacgat tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat
    10561 ttttagaagg attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca
    10621 atacaatgac gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa
    10681 cgtcaattga acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat
    10741 ctgtttttgc attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct
    10801 tgattgcttc tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg
    10861 tcgaacgttt aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg
    10921 aattgtattg tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc
    10981 caggagtttc tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga
    11041 tgatcatcaa tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc
    11101 catttgggtt tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct
    11161 tgatccatgc attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga
    11221 ccccgatcgt attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag
    11281 gagaattcaa caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca
    11341 tctttattgc attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg
    11401 taccaggatt attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc
    11461 cctatttagc ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg
    11521 ccattcggct ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg
    11581 ggatcggagc atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt
    11641 gtgcaatcat cgcattcatt atctggttcc catttatcaa attctatgat aataaattat
    11701 taaatgaaga aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa
    11761 gaaaaaagga actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac
    11821 tagtatataa ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat
    11881 aaggttcatc tacaccattg attttttgat agggatccac gccttttccc gcaataacga
    11941 ctgaatcttg cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt
    12001 cataatgaac gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt
    12061 tcggatcttc aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata
    12121 gcacttttcc aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa
    12181 caattacacg gccatctgga tgttcatttt tggcaaattc cagcaatgtt ttcaagctta
    12241 gataattatg tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca
    12301 ttcggcctgg tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg
    12361 ttagtgctgc ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca
    12421 aaatatcata agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac
    12481 cgtgagtacc tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag
    12541 tgatcgttgg aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt
    12601 catgatttaa aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt
    12661 catcaaatgt cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc
    12721 cgacatctaa agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca
    12781 tcactagatg agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg
    12841 actcaggcgt tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag
    12901 tagaaagcaa tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg
    12961 cagcagttgt tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat
    13021 agtcgtaaaa agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc
    13081 ctactgctga attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg
    13141 ctgaatccaa atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat
    13201 cagcttttct tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat
    13261 gccagcctct tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga
    13321 tgtcttctaa agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt
    13381 atagcacact ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca
    13441 aattttcttg acacagggga caagcggatg agtattttca taaacaatga actgactgta
    13501 atagatagga gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg
    13561 ataagactat tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa
    13621 ataatcggcg gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc
    13681 ccaagactga acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc
    13741 acttttggaa caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa
    13801 aatactgatt ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa
    13861 acagtaactg agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact
    13921 ccggcggaag ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa
    13981 agacctaaaa atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa
    14041 tgttagccat aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa
    14101 aaaaacaatt gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat
    14161 taaaatgata gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa
    14221 ccagcattag atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc
    14281 gccagtaata tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg
    14341 atgggcgaaa atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca
    14401 ttgtttctac tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc
    14461 tataattcat tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg
    14521 atgggcgggc taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct
    14581 catgcttctg gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta
    14641 ttggtttttc cttgtatgag tgtgatacga gggtattttc aaggcaatca agaaatgatg
    14701 ccttatgcgc tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct
    14761 acattcatca tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact
    14821 ttcgcggcat ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa
    14881 catcaagcgt atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg
    14941 aaggaattgt tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg
    15001 gtaacgatct tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc
    15061 gagtattcca atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag
    15121 ttgacgatgg tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt
    15181 acagaagctg ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta
    15241 cagttgtttt cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg
    15301 ctaaatacat tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt
    15361 tttgtaggac tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt
    15421 gaaaacaagg cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa
    15481 tacccagcca ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt
    15541 gctgtgtctt gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt
    15601 tttgtctggc gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg
    15661 tggatcacaa agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta
    15721 ttgttgattc ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa
    15781 atccgcttgg cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa
    15841 ataaaataag tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg
    15901 cgtttagata agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggattg
    15961 atcaaaaaag gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta
    16021 aatgaattcg aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt
    16081 tactacattt tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa
    16141 accgtaatcg atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga
    16201 ttagataaag acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag
    16261 ttactatcac ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg
    16321 acagaagaag atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca
    16381 ttgccggcaa aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt
    16441 ctgattttgc atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa
    16501 gaagtcttat atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat
    16561 aaagggcaat atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg
    16621 aatggatagc taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc
    16681 cgtctgactt tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc
    16741 tggaacaccg tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg
    16801 ttccttgtga ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac
    16861 gctgaattca caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa
    16921 aaaacggcaa aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac
    16981 gctgatttca caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt
    17041 ctcattattg aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa
    17101 aaaacgaaag gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct
    17161 gatggctgta ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa
    17221 tgtcgtagca ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga
    17281 aagtccttgg gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct
    17341 catcactaaa aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc
    17401 agtcgtattt gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca
    17461 aacgatgtcc ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc
    17521 ttttttaagt cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc
    17581 tgtcaattgg tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg
    17641 gaaagggatt ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac
    17701 ttattatgaa gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac
    17761 gataccacaa cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt
    17821 ccgagctgat tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt
    17881 gactcaagtt ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat
    17941 gacggctgct gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa
    18001 tacgatcgcc cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa
    18061 aaaaagaaag tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct
    18121 aatataatga ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc
    18181 atctctttta ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag
    18241 ttcagtatat ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg
    18301 cttttcgtca ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct
    18361 ttatatgctt atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt
    18421 tgtttgatta cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat
    18481 cttcttcagt taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc
    18541 aatattattg tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg
    18601 gcacggatcg atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct
    18661 gttccaggta ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc
    18721 aatgctttac tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa
    18781 atccaaagta atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc
    18841 ggattagcct cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg
    18901 cctattgcga tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc
    18961 ggagtaaaag aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa
    19021 gttatggaag aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc
    19081 atgcggaggt tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat
    19141 gtatcaaatc ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg
    19201 aatcgaaaaa gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga
    19261 aaagaaaatg aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa
    19321 ctatgtttcc aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta
    19381 cgctgaaaaa gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg
    19441 aaaattatat gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa
    19501 caaagcttta ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc
    19561 agaaaaagtc ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg
    19621 acaaggcttc aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc
    19681 tattgatctg gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat
    19741 cggactgtta cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc
    19801 aacgagcaat aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca
    19861 aggaccgtat gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc
    19921 aaaagcaatt actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt
    19981 atcaaatact tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga
    20041 tccagaatta ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga
    20101 tggttcagat gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc
    20161 ttggaataca ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat
    20221 tgccaaacgt gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt
    20281 caacactgac agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt
    20341 agatggattg aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc
    20401 tttggacaag gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt
    20461 ccgacaagaa taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct
    20521 cttgttccga ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta
    20581 aaaatgtgga aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt
    20641 cacaaactct tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga
    20701 caaaatatga ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt
    20761 gcccaacctt tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg
    20821 ttagggaagg ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg
    20881 tatttctgga tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa
    20941 acagtcaatg aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga
    21001 ggctggaata tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta
    21061 tctggtactg ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt
    21121 tttttagcta ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa
    21181 tacagtatga ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt
    21241 tttatttcgg ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata
    21301 attttaggcg tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg
    21361 acggtttgga atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa
    21421 agttatgaat aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg
    21481 gaaatacgcg gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca
    21541 tacttgggaa caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac
    21601 aagcagaatg ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg
    21661 gaattccgaa aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct
    21721 gatgtatgta tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat
    21781 caaagatctc tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact
    21841 accggagtat tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc
    21901 aaaattacta gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg
    21961 aagtattttt attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc
    22021 attcaatgga ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca
    22081 tgataataaa atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct
    22141 gaagaaaaat tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca
    22201 agaactgtct gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga
    22261 aacgggaaaa aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct
    22321 tatctttctt tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc
    22381 catggatcaa acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata
    22441 tgaactgaag gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt
    22501 agaagacatc tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc
    22561 acagatcaat ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag
    22621 cgaaggaagt gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa
    22681 tacgaatcaa gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt
    22741 ctatttgtat caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga
    22801 tttggaaaag atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa
    22861 acacggtatt gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg
    22921 caaacgggta aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa
    22981 acagattgaa gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca
    23041 aaacgtgaat gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa
    23101 taatgagaca ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa
    23161 gtgttattgg tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg
    23221 caaagtttag gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta
    23281 cttgaacagc gacagattga tttagtagta acggatgtga caatgccaga aataaatgga
    23341 ctggaattta ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca
    23401 ggataccaag aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg
    23461 atgaaacccg tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta
    23521 gatcagcaaa atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa
    23581 tggttgaatg aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag
    23641 aagcagcata ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga
    23701 tggttgcaag aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg
    23761 atcacgttgt tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac
    23821 tttcctgatc aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc
    23881 cctgaaacat tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa
    23941 gacgagcatg ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca
    24001 agtttcagac gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat
    24061 ttttttgaac agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt
    24121 ttactgttta tggagattca acgggaattg attgatctgg aagatgagga atatttacaa
    24181 ggaatcgaaa aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca
    24241 ttcatcaaaa agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta
    24301 atcctgcacc agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat
    24361 ttaaatgtca tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct
    24421 tatttgaacc atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac
    24481 atcaatgaga tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc
    24541 aagaaaatcg tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaaa
    24601 aaaaagccaa tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg
    24661 ttcttattac ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag
    24721 ccagtttctc ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat
    24781 ttttcttaat actcgaaact aaacagcttt ttcacgacct ctccactaaa tggtgttcaa
    24841 tcagctgacc ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt
    24901 tgtctctcta atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca
    24961 aaagcttgca tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc
    25021 ttggctgttc ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt
    25081 ttgataagga aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta
    25141 attggtaaga gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat
    25201 attctctgtc catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg
    25261 cttcaatcgt caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca
    25321 taccgataac atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga
    25381 tataccaagc ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt
    25441 cgaaggtctg agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat
    25501 aactgaacaa gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt
    25561 ctatcgcggc catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg
    25621 ttgggggctg attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt
    25681 ttccgatcag tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt
    25741 ctgcataatc ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat
    25801 cttttgaacg cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct
    25861 gctgatattt cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa
    25921 aatcgctata agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat
    25981 agccatatgt caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa
    26041 ccgtagcacc tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt
    26101 ttacagcagc atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat
    26161 caggagacag ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca
    26221 gcatttcttc gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg
    26281 tatcccaaaa cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat
    26341 catagtggat tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca
    26401 cccgatataa attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta
    26461 tacgatcaaa ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt
    26521 ggttttgttc tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa
    26581 atcggatagt ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt
    26641 tggaaacagc taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag
    26701 aaaaatcagg atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat
    26761 ctataacaaa gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc
    26821 tgtaatcaat agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat
    26881 aacgtaattg acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag
    26941 aactttgagc atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca
    27001 tatgactgaa atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa
    27061 cgcggtcatc aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat
    27121 aattcatccc aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct
    27181 gattcgctgt tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt
    27241 gattgtcttt tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct
    27301 aaccaagtga ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg
    27361 gttgtcagtg gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa
    27421 aaatcttcag ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca
    27481 acatcgtttt cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca
    27541 tctaatgtcg cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat
    27601 gcttcaaggt aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac
    27661 aaataagcga tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct
    27721 tggaaattat cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg
    27781 acgacaggga attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca
    27841 agagcataat aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt
    27901 agaaagtcaa gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat
    27961 cccttgctaa aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt
    28021 cgtgtttttg taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct
    28081 ttcccgcgaa cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa
    28141 gtgattctgc ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct
    28201 acaggtaatt caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat
    28261 ggttgagtca tataaacacc tcttaatgat atatcatatt caaataatac cataatggat
    28321 gaaaaagtaa ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat
    28381 aatctttaaa gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc
    28441 aaaatcggtg cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga
    28501 ctgggcaaag aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca
    28561 aaatgacgga accacctttt tattgacggg agacattcca gcgatgtggt taagagactc
    28621 tactgcacaa gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat
    28681 gatcagcggg ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc
    28741 cttcaacgaa gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg
    28801 gatctgggaa agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct
    28861 tctttacaaa aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa
    28921 aaaaatcttg gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt
    28981 acgagacacg acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc
    29041 ttttacagga atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata
    29101 tctggtacca tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca
    29161 aacgatactc gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca
    29221 agaggggatt cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata
    29281 tgaagtagat ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat
    29341 cgccgcacct tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag
    29401 aacattgttg agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg
    29461 atcctcccat acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac
    29521 aacgtcagac aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg
    29581 aacacacctt atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg
    29641 gttttcttgg gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt
    29701 agaaaagtag gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg
    29761 ggatcgtgaa tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga
    29821 cgatctacta gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca
    29881 aacgattatt ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca
    29941 agcaatcaat gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct
    30001 tatcagctcg gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg
    30061 gggaacccct gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc
    30121 tcaattaatg aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat
    30181 tggctttgat aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg
    30241 gtggaaaggc cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa
    30301 cgggaatgag attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc
    30361 ggatgcagaa caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca
    30421 accggtacaa aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta
    30481 cgagtttatt cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga
    30541 agacttaggc tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt
    30601 agccaataca gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca
    30661 gctagaaaat attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc
    30721 ccatgatcaa ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat
    30781 ttgcggttgt tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga
    30841 tgaagttgga aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac
    30901 gaccggattc ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa
    30961 aactggtgaa gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc
    31021 tgaacaacta tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa
    31081 aagtggggcc actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt
    31141 gccgaaagac cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt
    31201 agaaaacatg ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc
    31261 agaagaaaag gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca
    31321 tttgacgatt gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt
    31381 gaataatctg catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca
    31441 acctttctgt gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt
    31501 agtagataca ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc
    31561 tgcagacgag cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc
    31621 agaacgatcc aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga
    31681 tagtaaaaaa atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg
    31741 tgtcttgttt cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt
    31801 ggtgaagcgc ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca
    31861 gcaagcattt gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt
    31921 gaatgaatat gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg
    31981 agaactaggc gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg
    32041 gttcaattac agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc
    32101 atacgcagca cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat
    32161 ttcaaaacaa caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg
    32221 cagcaagttt agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa
    32281 attggaaata actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac
    32341 gaagcaagcg gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga
    32401 ggaaaacgaa tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca
    32461 gatggtggag aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg
    32521 gatcaggtat ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc
    32581 ggcccgattg gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa
    32641 aaagggtggg cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct
    32701 gcttggacaa cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa
    32761 ggaaaaaata gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc
    32821 aatgaagaaa ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc
    32881 catccagatg accattatga aggtacttgt ccttatcata aagattgttt agaaggatta
    32941 gcagcaggac cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat
    33001 catcctgttt gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg
    33061 acgttagcac ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt
    33121 caaaaaatcc gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta
    33181 agtgaatata tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg
    33241 ttagcagaac acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta
    33301 tcattatctg ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag
    33361 ctttaccgac tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga
    33421 gcatcagtcg ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct
    33481 tatgagctaa atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt
    33541 gtgtttgttc agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt
    33601 tatcatctac gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg
    33661 tcatatcttt ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt
    33721 gttccaacat tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac
    33781 cgcttgtttc tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat
    33841 aagcagctaa atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca
    33901 ttcctgcagg cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct
    33961 tcgcatctcc taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga
    34021 tctggtcatt gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt
    34081 ttttacgtat acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca
    34141 agtcgcgaat tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg
    34201 tactcctttc agtgcaatcg cagactaatt gtaccatttt taaaacatat ttaagaaaaa
    34261 acttacattt ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat
    34321 tgaactacaa cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga
    34381 atcaaataga caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc
    34441 tgctttgatc agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg
    34501 aaagcgcatc tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg
    34561 tgtcttagtg acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt
    34621 aaaatcagga ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac
    34681 actgtacgcc caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga
    34741 tgcagtaatc tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg
    34801 gattgcccgt gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag
    34861 tacgatcact cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg
    34921 aaaagcgaag gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat
    34981 cttgaccatg tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc
    35041 ttccagaaag tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt
    35101 ggcaggaatg ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa
    35161 tgatcggaaa aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca
    35221 agaacaagca gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc
    35281 agattctggt tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga
    35341 gtatggtctt gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga
    35401 tcaggattat caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa
    35461 tgcttcagat ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg
    35521 tgtacaagca ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac
    35581 acagatgcgt cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact
    35641 ggaagctgga tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc
    35701 gcataatttt gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag
    35761 tttgaatttg cctactgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa
    35821 agcagaagaa ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg
    35881 tggactggaa aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa
    35941 tgatggtcag atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt
    36001 gatcgtcgat aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga
    36061 aatgaccagc atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc
    36121 aggttatacg atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc
    36181 aaatgaccaa tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta
    36241 cgaagaatcc agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt
    36301 cttcaaatca gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc
    36361 agatgcctat gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa
    36421 ttctaatgga gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa
    36481 agaagggctg aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc
    36541 ggctaaagac ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc
    36601 atgttacaat agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg
    36661 atagcgcgaa tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag
    36721 aagcgtacga gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag
    36781 cgatgcaaat cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg
    36841 caatgaaagc acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga
    36901 tgcaaaaaga gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac
    36961 cagttcgcga cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga
    37021 gacgttcggc cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa
    37081 caccatttac caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc
    37141 aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag
    37201 aacaccgttt attcagaaat aggagctacg acaaaagtct tgtcatagct cctatttttt
    37261 gtcattcatt tttcaagcgg tagaaatagc ctttttgagc taacagtttt tcgtaagtac
    37321 ctatttcaac gatttttcct tcatcgatca caacgatttt atctgcctct ttgatcgttg
    37381 aaagccgatg agccacaata aaagtagtgg gtgtttctgt caggtaattc agggcttgct
    37441 ggattttttt ctctgactgg ttatctaaag ctgaggttgc ttcatctagc aaaatgattt
    37501 ttggctgacg tatcaacgct cgtgcaatgg agatacgttg cttttggcca ccagacagct
    37561 tgttgccatg ttcgcctact aaggtatcca gaccatcagg aagctggtca atcagtgaag
    37621 aaagttgcgc agcttcgatg acttcatcta aacgttcttt tgatacgttt ttcaagccat
    37681 aagtgatgtt ctctttgatc gaagcggaga acaacaaagt ggtttgagga acaacggcta
    37741 aataatttcg cacacttcgc atatccagtt catccaaagg ctgatcatct agaaaaattt
    37801 ttcctgaagt cggctggata aatccaatca ataagttaag gagtgtagat tttccagagc
    37861 cagatggccc aacaaaagca attgtttctc cagctttcac ctctagagaa aaatgatcga
    37921 taatcggttt gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga aaatttccat
    37981 ccaaatgttg gatttttttc ttaccgtgat aagtttctac atcgccagca atcaacagtt
    38041 cgccaataga agtgatagat tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc
    38101 cagaaacgga attgacgatc gtcgtgaaat acgtctgata catcacgaca tcgccgggtt
    38161 ggatgcgccc ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc tgaaatatct
    38221 gaaaactagc ccagctgact gatccgaaaa cagattgcag gatatctaag tggaatcctt
    38281 tttcatagat ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt tctaacccat
    38341 gagctcgagc aatcggtgct aattcgatca cttcgatcac ttttgcagaa gtctcttcca
    38401 tttcttgcct gaagtctgag ttggactgtc tgatcctttt acggaaaaag agagtaacga
    38461 atgcggcgag aggaacagtc aataagaaaa acatgaaaac aacaggactt ttataaagcg
    38521 tgatacctaa agctacgaat aaattgacag cgatatttaa tccgctgaca aatacttgag
    38581 aggacaacgt ttggatagct tctacatcac ggatgatctt tgactggatt cgtccagatt
    38641 gcaattcttt ttgataagga atagatagtt cttggatttt cttgataagg gtcgctctta
    38701 gtccagcttc tacttgacgg atcgaagtgc tatggtattt tacatgaaaa taattcgtca
    38761 atatattttg accaatcaga caagccatca aaaaagcgtt gaaataaatg gcctgcaact
    38821 gagaagaatc tttcgatgaa gctgcattga ttacattggc aatagcgatc ggcattaacc
    38881 aagatggaag atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc caataatttc
    38941 cctgataaag atgcagcaat gtctgaaaag catttgcatg atatttttga taacttttct
    39001 gtattcgttc ctccataatc ttctccttaa caatcttggt aaataaactg caagtgttct
    39061 attgcttcag gaacataaat ctcaggagta gcttcaagag tggcgtacat tagaggacgc
    39121 tcatatttga tataatgcag taccgggcgg aaatctaaac agcccttgcc aacaggtacc
    39181 atgcggatct ttccattttc catgatatag tctttgagat gaagcgctgt aatataacca
    39241 tgtaaattgt ccaaggcacc tcggatcact ttttcctgat cagcatgatt ttctttatgc
    39301 atcaaattcg cacaatctaa aatgattttt aaatttggtg aagctacttc atcgattaaa
    39361 cgtttagcta actgatagtt atagattgga tgattgatcc ctgcttcaac agcaacagtt
    39421 acgccaaaat tttctgcatg agcgactaat tcaatgatag aatttctggc gatctgataa
    39481 gcttcttcag taaaattttc ttttgtgtaa ccgacagaga cactaccggt ttccgtagca
    39541 atcatcttgg cttgataggc agagcacaga gcaatttgct gtttgaaagt tgccaatgct
    39601 tgtgcacgaa cttcttgatt tttgctggat aggttgatat agcatcctaa aatggaaagt
    39661 tcgatattgt ttttttggaa gtattcacca aaataggcag cactcccaag tgtcatgtca
    39721 gttaatttaa gttgttcagg aaatgctttt tgtggtgcaa attggatatg ggacaagtga
    39781 agctgtgata ctttttgaac gatttcttca cgatttcgaa catctaaatc gtgggcacgg
    39841 atgcctaaat ttaatggaaa catctcaaac tcctttttgt aaataattag caagaggcag
    39901 acgggattct tttatcaatt caacaattat tcgggcaact ttatcagcgc cttctggact
    39961 aaagtgggta ttgtctttta gtccttctgg gtaattttta caagtatcag gtataagatg
    40021 caggtagaat ttttttgtct cttcttttga gaaggtctgg aacaattctt gtgatcttga
    40081 gaacacatct aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg cttttggata
    40141 gtctccaagt atatctggat tcaatgttcc gttttctaag taatctcgtc gcgtcacaga
    40201 tgtcagtaag accatttgga cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt
    40261 ttttaaatag ctaccgaagg gaagcgtacc tctttcttct tggattttct ggtcgttatg
    40321 tccaaattgt acaaataaga aatcattcgg ctgaaactgt gaagctagac gggctaacct
    40381 tccttcctgg ataaatgatt tggtgctgcg tccattcttt gcttggttat tgatctggat
    40441 ctcgggtgaa aaataagcag agattttttc tccccaacct gtctcgggac gcttgttttc
    40501 atctttttct gccgcagtag aatcaccggc aatatggata gtaggcatgt tatctcttct
    40561 ttcataaagg tttataggca atgaagcaga acagcaagag aagaagacgg agcaaatttg
    40621 gtccgtcttc ttctctaaaa cgtggataaa cggattccgt tatttcacta tcctgttttt
    40681 gattcgttac tgttactatt aatcaattag gcaaaaaagc caaagtaatt tttagcattt
    40741 gtataactaa tcgcttgaac tagacttcct aaaagttgtt catcttcagg tgcttctcct
    40801 cgcatgacca tttcgcctaa aaattcacaa agaacacgac ggaaatattc atgtctagtg
    40861 taggacagga aacttcttga atctgtcagc atgccgacaa aattccccaa agcactacca
    40921 tcagcaaatt gtgtcaattg atcgcgcata ccagctcgag tatcgttgta ccaccatcca
    40981 gaacctaact gcagttttcc attctgttct ttttggaaac agcccatcaa agctaaaatc
    41041 gcaggatagt cattcggatt caatgaataa aggatcgtct ttggcaactg atccgtttct
    41101 tctgcacggt tcaataattt ttgcagagga atagtcaaag aaagatcatt cattccatca
    41161 tagccggtat cgggccctaa tttttccaac atttgtgtat tacagttacg ataagcatgg
    41221 atgtgcagct gcatcgtcca attatgagaa tgatagaagt gggtcaaatg tatcagtgtt
    41281 tctgtacgat aagcatcgat ttcatctgaa gataatagtt tgttagctcg tgctttttga
    41341 aaaatctctt ctaatactgt ttcatccgct tctttatagc gtaagatgtc taaagcgtgg
    41401 tcggaaacac gacatccatg ttgatggaag tattctatcc gctgccctaa gacttctatc
    41461 agttcttgat aggtggtgat cgtttggtca gctgcttctt ccagtagtgt gacccatgca
    41521 ttaaatcctt ctttttcaat attgagtgct ttatctggac ggaatgaagg tagaaccttc
    41581 aaatctggtt cttcatgagc aagcaactga tgatagtgca agtcgtctgc gggatcatct
    41641 gttgtacaaa tcacttctac attcgatttt tttgcgattt cccgacggcg aaaatcagga
    41701 gctgccaaaa tttcatttgc ttgtgtccaa attttctctg cattttcttc atttatcaac
    41761 aagtcaatac cgaagaaacg cttcaattca agatgtgacc aactgtaaag cggatttccg
    41821 ataatttttg gtacggtttg acaccaagct aaaaattttt caaaatcaga agcatttcct
    41881 gtgatttttt cttcaggcac gccgcaagct cgcatcaagc gccatttgta atgatcgcct
    41941 cctaaccaag cttctgtcag attgctgaaa ttgttgtttt cgtaaatttc ttttggatct
    42001 aagtgacagt gataatcaat aatgggcatg ttttttgcgt aggaatggta aagctttttc
    42061 gcccaatcgt cttttaataa aaaatcatca gataaaaaca taaatcttcc ttctttcttt
    42121 ttatactgtt ttatgaaaat caaagtgtta ctccgttttt aaaaatggca atttctcgaa
    42181 cgtcattttg ttcatttttc gtttcttctc cactagctac agcgatgatt ttttgaacaa
    42241 acgattctaa gattacttcc attggttgtt ctaataagac gcctgcatta aaatccatcc
    42301 aatgaccttt gcgttcaaaa agagttgtat tcgtagatac cttgaccgtt gggacatatg
    42361 agccaaacgg agtccctctt cctgtggtaa acaagaccaa ctggcaatcg gatgaagcaa
    42421 gagcagaggc agctacaaga tcattcccgg gagcttgcaa taaactaagc cctggttttc
    42481 tgattttttc gccatattgt agcacatcaa cgacaggaga gcttcctgat ttttgcgtgc
    42541 agcctaaaga cttatcttct aatgtagtga tcccacctgc tttattacca ggagatgggt
    42601 tttcgtaaac tggttccccg taagacaaga aatactgttt gaagtcattg atcaagtcaa
    42661 cgattgagtc aaaaacttct tcattttccg ctcgtgccat caatacttgt tctgcgccaa
    42721 acatttctgg cacttctgtt aagatcgtgc tgcctccttg agcaatcaag tagtcagaaa
    42781 atgcaccaag taaaggatta gcagttattc ccgatagacc atcagaaccg ccgcatttca
    42841 aaccgacatt caacttagag agcgggactg atatgcgctg gtcattttcc gccgctgtta
    42901 aaagctcttc taatagagcc acaccttgtt cgatctcatc gtaaacatct tgagccacta
    42961 gaaatttcac tcgttcttca tcatattcac ctaaaatttt tttgaattca ggaactgtat
    43021 tgttctcaca acctaatcca aatactaaaa ctcctccggc attgggatga tttactgcgt
    43081 ccgctaaaat ttctcgagta ttttgatgat cttttccaag ctgcgaacag ccataaggat
    43141 gctttaaaat cgtaatatgg tcaaactgtc ccagatctgg atgtttttcc ttgaactgtt
    43201 tgacgatcag ttcagcaatg ccattcacac acccaacggt aggtactaaa aacaaatcat
    43261 tacgaatccc tacttttcca ttttttcgca agtaaccttg aaatgttcgg ttctcaaaaa
    43321 ggtaatgact tggatggaca tctttttcat atcgataatc caattcacca gaaagattgg
    43381 tttttacatt atgagtgtgc agccattgac cggcagttac gtctgtcgtc acatgaccga
    43441 ttggatagcc atatttgatc acgttgtctc cagctttcaa atcacccaat gcaatcttat
    43501 gaccttgcgg gatatcttca agagtggtga tcgtttgctg atcaatcgtc agttgtgttt
    43561 gttttggaat aggggttaat gcgacgacca cactgtctcg tcgatttaat ttcattgttg
    43621 gttgcagggt ttcaagcatg gaccatcgtt cctttcattt cttgtaatac tgaccggaca
    43681 cctttttgat cgatctttgc agctatttct tgaactgtat ctgtcacatt cggtatttga
    43741 gacaaatctt ctccccaaag cgcagcgttt tgcaataatc gaggaatagc tgttccatta
    43801 tcagaccaag catcagtaaa ttcgcttatc gtcttttcat catcatgtgg ctttacttga
    43861 tcgcctcgat acataagaag tagcgcagct agtgaaacag tgattaacgg tggtacctga
    43921 ttttgttctt cgatatactt cttgaacact ggaagcagac gggtgctgaa ctttgaaaca
    43981 ctatttaatg aaatcgcttc taatttgtga tgggcaaaag gattcaagaa acgttctttg
    44041 atttggtcag cataaatggc taattcatct ttaggtaaag aaagcatagg aatcaattct
    44101 agattgaata actgatcaac atatgcggag aataaaggat ctttcattac ttcttctaca
    44161 gtttccaatt ccgccaacaa agcaagaggg accatcgctg tatgcggtcc attaagcaaa
    44221 tgcaccttac gttcacgata aggagtcata tcatctgtga tgatgacatt caagccagcc
    44281 tgtttgaatg gtagttgatt ctccaattct ttaggccctt cgatcaccca aagcatgaat
    44341 ggttctgctt tgaccatcaa ttgatcttca tagttatgcc gttgattgaa aacacctgca
    44401 tcttcgcgag gataacctgg gacgatgcga tcaactaaac tacaacagaa aacattttct
    44461 ttttctaacc actcaacaaa agcaggttct aaatcccatt tttctgcgta gcgtaaaatg
    44521 atttctttta gacgttcgcc gtttctgtca atcagttcgc aaggaatgat ggtaaagcca
    44581 gctttgttta atttgaaacg tcggtataaa aaggctgtca gtttgcccgg gaagctctgt
    44641 gggggagtat ctgttaaatt atcagtctcc acgaattgga ttcctgcctc tgttgtattg
    44701 gagacgatat aggccaagtc gtcattttct gctagagata gataagcatc ccattcatca
    44761 taaggattga tcatttgttc tactacggtg ataatctcgg aagtatcaac gatttcgcca
    44821 ttgctgagtc cttgtaaaat gaccgtatat aaattatctt gctggttcat catatttcca
    44881 agaccttgtt ctaaaggttg aatgattgca acattacctt taaacaattg ttggttattc
    44941 agctgctgaa tctgccaatc aataaatcca cgcataaaat tgccttcacc aaactgaaga
    45001 acttttacag gtgcttcgtt ttttttcact aattcttttg tcagtttttt catataaact
    45061 tcctttctgt gttatgcaca cgttaacatt tttatataaa attacttgta gtttacaagt
    45121 aattgatcga ctcacgaagg atcaacaagg tggagagctc aattttagat tgttcgatcg
    45181 tcctgccttc gatcaaatct agtaaaagct ttgttccttc ttgacacatt tctttcaaag
    45241 gcttacgtac ggtggtaagc ggcggattga gataggagac gactggagaa tcatcgaacc
    45301 cgatcagaga aatgttctgg ggaacacgga ggccagctaa atgtacagct ctcaatgcac
    45361 caatcgctat gtcgtcgttg ccgcaaaaaa ccagagtagg caggtcagaa agtgataata
    45421 ggtattccat gttttggagg ccgctttcta agctgtaatc tcctgttaaa aaataggctg
    45481 gattgacagg gatgctcgct ttttttagac ttttcaatac ccctttttcc cgttcttttg
    45541 tcgaacgaaa atttttcttt ccgccaatat aagcgatctt cgtatgtccg catccaatac
    45601 cgtaatctac tgcctgcata acaccggccg catcatcggc agtgacgttg ataaccgctg
    45661 gatcattgat gtgacgattg acaacaacga aaggaaattc tttttctttg agatagtcga
    45721 tgaaaggctg atcatcatca ctttgactca taacgatcag tccatcgtag cgttgcaggt
    45781 tgatttctgt taaatcaaca acatcgatac tttcaacaga taaactgtaa tttgtgtcta
    45841 atactgaatg gatgcctgca atggaatctg ctaggaaatt tgatgaagtt cctttatgca
    45901 aacttgagaa aaatagtccg attagatatt ttttttgatt cactaaacct tttgcactat
    45961 agtttggcga ataattcatt tccttagcta gttgtcgtat tttttgtttt gtttcatttt
    46021 ttattaaact gctgttattc aaagcacgag aaactgtcgt atgagaaacg ccagctgctt
    46081 tagcgatatc tttgatcgtt accattccat cattttcctt actttttcac tttttgttaa
    46141 cgcattcatc atattacgat aactacgata agtcaataga tctttgatat gaaaagattg
    46201 cacacgttaa aaattacttg gatttttatt tttaattatg ctattcttga aagcggggaa
    46261 tgctcattta attagatgag tatcaatttt acagaaaaat gaaagccttt tattttaaaa
    46321 ggaggagagg aagaaaatga agaaaagtac agtattttat aaaatatttt taccgatgtt
    46381 tttattagga gccgtacttg tcataggatt tagtttattc atttaccaga atacctatga
    46441 atcgatcgaa tcgagttatt taacagacaa aaagaatttg ctcaggcaag ttaaaactaa
    46501 cgttgaatgg aaaattagaa cgattgaata ttcgttttct acgtatggtt cgaccaaaaa
    46561 tttctcggac atattcaagc atccgctgac atatacagat tactccactt attctgaaat
    46621 caggaaagaa ctaaatttta tagaaacgat tgttatggat gataataatt atgatttagt
    46681 cagtttggct ggtaaatggg gagtaatcaa tggatcgttg agtcaactga ccgaagagga
    46741 agttgaaaag tatcaacaga agtacataaa taacaaaaat aatttatttt ggcaaaagaa
    46801 aaacaaagga atcgaaatgg tgatttcttt gccaatgttt gaaaaagaaa aatatgcatt
    46861 gggaattgct acgatagaga aacatacaat agaacaagtt gttggtgaaa gtggagaaga
    46921 catactttct attgaaaacg aagttggcaa cctttttact actcagacag taaaaaataa
    46981 agagcaacca actgtattaa cggacgtgga tctagattac aatcagctga aagtgataag
    47041 agaaaaagat aaaacgtata tagtactgaa atcagattat aataattgga tttacagatt
    47101 agaagttgac tctagtgcaa ttggcgcaat gatccgcaat ctgagaatcg ggctagttgc
    47161 agtgtcttta acactggtag gactcattgg tcttctttcc tacttatttt cggacagatt
    47221 tgcccgtccg atttctcaaa tacaggaacg tctgaatctt aaatcaaaag gattcagtgg
    47281 gaaagaacta gatttggtcg ctcaatcagt cagtcggatc ataggagaaa aagaagcgtt
    47341 aagtgcacac ttagttaccc aaaagccaca gctggaaacg ctatttgtac ttagtttatt
    47401 tagaaatcga gtagaaaggc gagaactaga tcagcggttg cagcaatttg gttatagttc
    47461 tcaaaatgac tgctattata cagctttggt tcaaatcgat ctgttagaag atagtcacgg
    47521 tggggagcgt gatttgctac ttttagcgat caacaacatg atcacggaaa ttgtgccaaa
    47581 ggaaaatcga atgctgccga tcgttttgaa tgaagaaatg caagccacta tttatcgaat
    47641 ggataaagat gatgcagaag caagcaaaaa agtcatggag tattgccgac tcattcaaaa
    47701 acgtgtaaaa gaatacttga atttaactat cagtataggc atcagtaacc ggttcgaaac
    47761 gctgaatgaa agcaagcaat cggttgatcg ggcaaaagaa gccctgtatt atcgtgtaaa
    47821 tactggtcca agttcaatta ttttttatca ggaaatcgtt cctgctgctc atgaaaaaac
    47881 acttattaga tatccgatag aagaacaaaa tcgattgttt gaagccattc gttctggaaa
    47941 tcaggaagtg aagaatttag tacatgaatt gattgatgca ttgtttgcac aaaataaaaa
    48001 tcctttgagt cgggaagtag tttcggtccg cttgatcaat gaactcgttc aattaggaca
    48061 gttattggga gtagattcaa aaaactttga taacatgaaa caaatttatg taaaagcact
    48121 gaacaattat catcccaaag aactagaaaa acttgtttta gaacaattag ttctgccaat
    48181 cacagaatgc tctcaagcaa tcactgatca tgaatttaaa aatttgtcgg agaatattat
    48241 gcatatcgtc tacacagaat atgatcgtga tctgtcttta gatgtgatcg ctgataggtt
    48301 gcattataac cctaactatt taagtaatgt atttaaaaag gaaacaggag aaaaatttgg
    48361 tgattttgtt caaaatcatc gtttagaggt agcaaaaaaa tggttgaaag agactaacct
    48421 ctccgtaaaa gagattgcag aaagattgca atatcgaaat ccgcaaaatt ttattcgttt
    48481 ctttaaaaag aaggaatcga ttacgccggg agaatatcga aaaaaatatt tgtgaatcaa
    48541 gaggctgttt ttcttgtgga ctgattcaag caataaaaga agttagaata accctagtat
    48601 ttgtatttat ggatagttaa atttcaaggc taagcacgca aaaagaactg tgacaaaagt
    48661 cttgtcacag ttctttttta cgaataaatg gcgttcaaga gacaggctat cgacaataag
    48721 gcataatttt gtgaaatatg ggaagctatt tttcaaaatc gcttcttatt gctcctgtgg
    48781 gttactcgtc gcaatgtttg tgagagtttt tatcatcgct caaggctgaa cgtctctttc
    48841 acaacctctg atacacggtg ttccaggagt agattcttcg aaaaataagc cgaaatttca
    48901 caaaaattta agaagcaatt ttcgtaaatg ccttcttatt tcttgaagct gaccacttct
    48961 gacacaacta ccattttgcg ttcagtacat aatcaccctt tgacagaacc tacgagtgct
    49021 cctttgacaa aatatttctg aacaaaagga taaacaatca gcataggcac tgttgcaata
    49081 acgataacag ccattttaac tgtttgtgct ggtggaatca catcaacggc actactttct
    49141 gcttgcatcc cacttgatac aatgacgatt tggcgtaaaa gaatctgtat cggccattta
    49201 gatgaatcgt tgatatacaa aatagcagtc atataagtgt tccagtaagt aacagcataa
    49261 aaaagcgaga tagtagcaat agacggtaac gctaatggca gcataatttt gaaaaataca
    49321 ccaaaatctg tgcaaccatc catttttgct gattcttcca aactatccgg caaggcttgg
    49381 aaaaagtttc tcataatgat catattcatt gcattaacag ccacaggcag gatcatggcc
    49441 cagtaagaat tgaccagccc tagagaacga acaactaaga acgttggaat catccccccg
    49501 ctgaacaaca tagaaaatac aacaagaaag ttgacgaaac ctcgaccaaa caaatacttt
    49561 ctggaaagac cgtaggccat cagtgaagtt gcacacatac tgacgatcgt tccgacacct
    49621 gtaacaccaa tcgaaacagc aagtgctcta aaaatggtcg gcgtagaaag aatgtagcga
    49681 tacgcgtcta aagaaaaggt agtaggaaaa aggataaact ttttcgcgac gacttcttgt
    49741 gtagaagcaa aagatgtagc gatgatattt aaaaaaggaa cgatacagat caaagctagc
    49801 aaaattagaa acgtattgtt gaaaagtgta aaacttcttt ctcctataga gactttcttt
    49861 ttcatataga taggctcctc ctcaaaataa attaaatgtt tctgtttcta tatgttcaat
    49921 ttatcatcgg tgaaagatta cgtatataat catttcctat gctaagtgtc agatagaaaa
    49981 aaatagaagg tttgctatgc gtagtttatt tgtgaaaaaa tacactttat cgttttgtat
    50041 agcacaaaaa tcattgctat gtaagcgttt ttacttacgg taaaaatgat tatatacgta
    50101 attcagatag ttatctatca tgaagtcata agcagcaaac attcacaaaa ctaaggaggg
    50161 gcttatggaa agtgtaaaga aacaagaacc atttcaactg acgctatcga aaaaagaaga
    50221 aaagctcaga agacgaaaaa aaataattgc aggaatcaaa acgaataaat ttttatatgt
    50281 gatgattctg ccgggagtcc tttatttctt gatttttagg tatcttccta tggggggact
    50341 cgttattgct tttcaagatt atcaaccatt tttaggaata atgggaagtc cttgggtcgg
    50401 tttcaaacat ttcattcgtt tgtttacaga accgacattc tttacattat taagaaatac
    50461 gttgatcttg ttcggattaa atatcttcgt gttttttccg atgccaatca ttttgtcttt
    50521 actattaaat gaagtaacga acaaacgatt gaaaaacggg attcaaacga tcatttacat
    50581 ccctcacttc atgtcttggg taatcattgt ttcgattact tatgtatttt tgaatgttga
    50641 cggaggggta ttgaatgaat tccttgcaac aatgggattt gaaaaaatca gttttctaac
    50701 atcccctgaa tggacacgca cagtatatat cggacaagtg atctggaaag agttgggctg
    50761 gtctacgatc atctaccttt cagcgataac tgcagtcgat acacagttgt atgaagctgc
    50821 agaaatggat ggagcaggac gcttacgcaa aacatggcat gttacattgc cggcaattcg
    50881 tccagtaatc attaccttgc tgattctaaa aatcggtcag acattagact tagggttcga
    50941 gcatatgtac ttgttattga attcattgaa tcgaagcgtt gcagaaattt tcgacaccta
    51001 tatttataca gctggattga agaatggaca gttaagtttc agtacagcaa tgggattgtt
    51061 taaaggggtc gtaggattgt ttatggtaat tttagctaat aagattgcca aaaagctagg
    51121 agaagacggc gtttactaag atagaaagag agggaaagat atgctgcagt cattagataa
    51181 aataaacgga aggctaaatc gtattttaca gttggtttat ttgaattttc tgtggggcgg
    51241 tttagtcatc gcaggtttgg gagtttttac tttcggtcct gcatcgtatg cgatggttgc
    51301 agtgattaga aaatggctgc gctcaaaaga aacgtttcct ttgacgaaaa cgtacttcac
    51361 atattttaaa gaaaactatc gtgaaacctt gctgatgagc tggatatatg gcgccatcgg
    51421 gattgtttta cttgtggatc tttctttcat acacaattgg tatcttagga tcgctttgta
    51481 tattgctgcg ttcatatatg tgctctcagc actctatatt ttcccaatca tggctcatta
    51541 tgattggaaa ggatattttt tcaaaatgaa aatggcattt ttattcggat tctcacatct
    51601 tcattactca ttagtgttga tattaagtat ggtagggatt tatctgatga tcatccaaac
    51661 aatgccaggt gttttaacct ttatcggtgg cagtctcttc ttttccgcag tcacgtggac
    51721 agcaaattct atattttgcc atttggaatg gaagaactca tatacagaaa aaacagaaga
    51781 aaacaaaaat attattaaaa cagtgaagga gaaaatttat gaaaaaagca acgctcgtaa
    51841 aattaggtct tagttttgca ggaattctag cacttgccgc atgcggcagc agttcaagta
    51901 acagttcttc agagacagaa gatggaaaag ctaaaatcac ttggttaaat attttgcatc
    51961 atgcttcacc gcctacagac acgattgtga acttgatcga agaaaaaacc gatacggaac
    52021 tgacattcaa ctggattcct gatgcttcaa aagaagaaag attaacaact gcattggctt
    52081 ctgatgaact aggagatatc gttacacttc aaattgcgat gttgaaaaac tcttctgtcc
    52141 gcaatgcgtt aaaatcaggc gtattctggg atgtatcaga atatttaaaa gactataaaa
    52201 atttaagtca aatttctgaa gatagaatcg aagctgcgag tatcgacggt gccttgtacg
    52261 gcgtaccgat acaaaaagac tacgctcgtg ctggcttagt cattcgaaaa gactggttgg
    52321 ataatctagg actagatgtt ccaactacca tggatgagct ttatgaagta gctaaacaat
    52381 ttacagaaaa cgatcctgat ggaaatggtg tggacgacac agttggtttt ggcgatcgtg
    52441 cgcctagtga tattcgttat accagcttta aacttttcac ttcttatttt ggagcaccaa
    52501 atggctgggc tgtagacgat aatggcaaat tcactccaga atttgataca gaagagtata
    52561 tgaaagcttt gaatttttca agagatctat ttgagaatgg ctatttggca caagactttg
    52621 cagttaccca aaagactgat cagcaagaac aatttgcaca aggaagaact ggaatctaca
    52681 ctggaatggt ggatatcaag aatctgaaga cgatgggtca aggtattcaa gatgatatgg
    52741 aattagtgcc tgtaaacaag atctcagatg gtgaaggcgg cggttatcat gtttggtcag
    52801 aaggaaatgg tattggcggc ttgctagcat ttcccaaatc tcaagtaaaa gacgaagcac
    52861 aattgaagaa attacttcaa tttgttgatg atcttttaga agaagatgtg tataaattga
    52921 tgactggcgg tatcgaagga acgcattatg aattgaatga tgatggttct atcgaattta
    52981 tcgatatgga tctttggcaa caagaggtgc aaccgttatc aagctctcgt cctagtgaag
    53041 tgacgttctc tttcaaagat acagaccctg aaaaagaatt ctctaatcaa ttgatcaaag
    53101 aaaatgaaga atttgctgta atggatcctt ctgtgccatt ggattcaccg acgaacaatg
    53161 aagtaggatc agaaattgaa aaaatcgtca ttgatgctac ggtgcaatac atcatgggac
    53221 agcttgacga agatggattc aaaaaagctg tagaagactg gaaagcacaa ggaggcgatc
    53281 gaatcacaca agagtacgag gaagcataca aagcagcggc acagtaatat agacgattgg
    53341 gacaaatact tcttggtatt tgtcccaaat cctattttaa tttttaaagg agagcaaata
    53401 atggaaaact ggtcaaagaa attagcaaaa agtatcatgg aaagaacacc tcgcttatat
    53461 gaagaaaagt ggtataaagg aaaatggtct tatgactatg gcgtcgtact taaagggttc
    53521 caattacttt gggaacgaac ccaagaaaaa atttattttg atttcataaa agacaatatc
    53581 gactatttcg tgcaagaaga tggaacgatc cgtggctaca gcgttgaaga atacaatatc
    53641 gaccatgtaa atacaggaaa gcttttcttt ttattgtaca aagaaacagg cgaagaaaaa
    53701 tataaaaaag cagcagaact cctttcccag cagttggcaa accatccacg cacatccgaa
    53761 ggagcttttt ggcataaaga aatttatcct tatcaaattt ggttggatgg cttatatatg
    53821 ggttctcctt tttatgctga atttatccag aattttagtg aagaaaaaga ttaccaagat
    53881 gttttgagac agtttgaaat cagttataaa catctattcg atccaaaaac aaatttgctg
    53941 attcatgcat gggatgaaaa gaaggtccaa ccatgggctg atccaaaaac aggtctatcc
    54001 catcattttt ggagccgatc gattggctgg tatttgatgg ctgcagcaga tacctatgaa
    54061 ttacttcatg aagaaacaga ttgttctttg ctgaaagaaa ttctagaaaa gacactggct
    54121 gcattacttc catatcaaga agaaaacagc gggacatggt atcaagtaac aacagaaaca
    54181 aaacggaaag gaaattatct ggaagcttcc ggcagtagta tgtttgttta cgcaatggct
    54241 aaaggaatac gtttgggact gctgaacaaa gaagaatggc ttccacaagt gaagaaatca
    54301 catcaaggat tacttgatga atttgttctg gaaacaaaag aaggctggct gaacttgaat
    54361 aaaaactgtc aagtagcagg gttaggcgga gcagatcagc gagatggttc atatgcttac
    54421 tacatcagtg aaccaattat ttgtaatgat caaaaaggcg ttggtgcatt cttgcaagca
    54481 ttgattgaag tggaggcatt ataaatatgt atgatatttt gacatttggc gcatcaattg
    54541 atgaattgaa tactgaagca atacaacaag ccattgatgc tgcagcaagt gacggaggcg
    54601 ggaccgtagt tgttccagca ggtgaatttc taacaggagc tttattttta aaaagcaatg
    54661 tcgaacttca tttaagcgca ggagcggttc tgaaattctc tgacgatcca aaagattatc
    54721 ctgttgttca ttcacgttgg gaaggagtcc atcgaaaagt ttatgcttcc tgtatctatg
    54781 ctcaaaatgt agagaacatc tctgtcactg gtttcggtac attagatgga aatggaaaaa
    54841 aatggtggca tacttttaga aatgaaccag ataatctcgc ttatcctcgt cctaaattga
    54901 tgagtttcca taactgtcat cgaattactg tgaaagatat taaattgatc cagtcaccaa
    54961 gttggacgat caacccgatt ctttgcagta atgcaacgtt tgataacttg acgatcttga
    55021 acccagcgga ttcaccaaat acagacggaa ttgatccaga atcatgcaaa aatgtgcgta
    55081 ttagtaattg ccatattgac gtgggagatg actgtattgc aatcaaggca gggactgaag
    55141 atacgtacga aaggatcgct tgcgaaaata tcacgatcac aaattgcacg atggtccatg
    55201 gacatggcgg tgtcgtatta ggaagtgaaa tgagtggtag tattcgtaat attaccattt
    55261 caaattgtat cttccaagaa actgatcgag gaatacgatt gaaatctaga cgaggtcgag
    55321 gcgggatcgt tgaagatatt cgagtatcca atatagtcat ggataatgtg atgtgtccat
    55381 ttatactgaa tctttattat ttctgtggtc cgcgagggaa agaaccgtat gtatgggaaa
    55441 aaaaggctta tccgatagat gaacggacac cagcattcag aaggatccat ttttcgaata
    55501 tcacggcaag gaatgtccat gcttctgcag gttttattta tggattagcg gaacaattca
    55561 tacaagaaat cacatttgac aatatcgatg tctcgatggc ccaagatgca gttcccggtg
    55621 ttcctgctat gatgacagga atagaggaaa tgagcagaca aggattttat attggctttg
    55681 cgggtaaggt ttcctttaac cagatgtcga ttgaaaatca cgagggacca gcatttctat
    55741 tagaaaatac taaagaaatc agtattgaga aattccaatc aaatcaatca gaaaatatgg
    55801 atgaactggt tgttcgtaaa aactttgctt atgcagttga atgatttttt aggtgctcca
    55861 tctagcagac atacccctag tggaaaacga ttataccgag aaaaacaaaa cggttttatg
    55921 cttgaatact atcaattgta cttaaatgaa ttggaaacag taccagccct ctatgcttat
    55981 ccagaaaaag aaggaccgtt tccgccggtc ctttacctgc attctcacgg cggagatttt
    56041 tcagtcggga aaagtgaatt gatccatggt gctccttact tagcttctcc atcttttgca
    56101 gaagtactga caggaatggg atatgctgtt tggtcgattg atgcttgggg atttgaagaa
    56161 cgcggaggta tttctgaaag cgagttgttc aaacaatttt tattaacagg aaaaacgtta
    56221 tggggaatgc gtatttatga tgtgatcagc ttgatcgatt atttggagac aagggaagac
    56281 acagatactc aacggctcgc aacgattggt ctttctatgg gaggcttatt aagctggtgg
    56341 gtgacagcat tggattctcg tgtcaaagtt tgtattgatc tagcggcaca ggtggacatc
    56401 gaaatacttt tgctacatcg ccgcctagat catcatgggt tttattacta tgtgccaaat
    56461 ctattgacag cttatacaac gttagcaatc caagaaaaaa tcgctccgcg ccctcgtctt
    56521 agcttagttg gaaaaaacga tacgatgtgc ttggacgaag gcgtactgaa gctgagaaaa
    56581 gggctggaca aaaagtatca atcaatgggt agtccagaaa acttttcttc tttttcttta
    56641 acaggcggtc atatggaaac acaagaaatg agaaatattt ggaaacaatt cataagggag
    56701 catttatgag gatgaaagac aaactgataa ttggaaagaa tgctatagat accccgacgg
    56761 attttgaaac tatccaagaa ggaatcgatt atttggcagg cctgccggat agccatccaa
    56821 aggtcttgat agtaaaagaa ggaagatatg atgaatatat agaaagcaga ttatccaatt
    56881 ttaaacttgt gggagcagga acagtagtta tctccggtaa tcgttttgca aaacaggagt
    56941 atcttgacgg tagccagtta gggactttcc gaagtgctac ttttttctta gaaggatcga
    57001 atgtcgtatt agaaaatctt cacatcgaaa atacagcagg tccaggagaa aaggtaggac
    57061 aggctgttgc tttattcaat tcaggagatc gcgtatcttt aaaaaactgt cgactatctg
    57121 gttatcagga taccttatgc acgggtccgt tgcctgattt gcaaaaagat ggtactcctt
    57181 tttgtacacc ggcttctgcc aatcctgagt attgtcgtca gacctatgaa tattgctgga
    57241 ttgaaggaac ggtggatttc atatttggtg gggcagatgc cgtttttcat cagtgtcata
    57301 tccatagctt agccagtaaa aatcctggat acatcacagc agcatctacg ccgaaaaatc
    57361 aaaaaacagg gtatcgtttt gaaagctgta ccttgactgc agaacctgat actagaaacg
    57421 tctatttagg aagaccgtgg cgtccgtatg caaatgtctt atttgaagcg tgtaagatag
    57481 gtgggcatgt tcacccaact ggttgggata attgggatga caagaaaaat gaacagactg
    57541 tcaaattctc tgaacgtaat aacaattatg aaacatcgat caaaagacct gaatggatac
    57601 atttagaaaa gcagggggtt cagaaatgaa aagaaaatgg gaactattgc taggaatggt
    57661 cggtggcagt ctatctttga ttttctttgg cgggttagca gttactttaa gtaatatgtc
    57721 tgcgtcagag tttaaaaaaa gttatcagtc gcttgctgta gatcattcaa ctttgtcttt
    57781 agaaaataca tttggattat tacaggacat gactggtctg tttgccgttg tgttgtttat
    57841 ttcacttgct tttttagcag tagcattgtt tttgactgct aaaggaaaat acttaacaac
    57901 tgccactggt ttgtatttta taacaggatt catattgttg attggcactc agtttattgc
    57961 gtttcctttt gcattttttt atttcgcagc tggggcattc agtttatatc gagtaagaat
    58021 gagaaaagga gcctgaatca tgcagactat caaagtgaaa aatcctattt taagaggatt
    58081 caatccagat cctaatatat taagagtgaa agagcggtat tacattattg tctcctcttt
    58141 tgaatggctt ccaggaatcc gagtatatga gtcgaaaaat ttagtcaact ggtcacatac
    58201 aacggatatt ttgaccactc aagtatcttt gcaaggaaat cctacaaatg gaagtatatg
    58261 ggcgccacaa atcagttatg ctgatggatt gttttatctt gtttacacag atgtcaaaag
    58321 tgcaggccgt ccgtttaaag atgtccacaa ttatttgatc actgcatctg agatcacagg
    58381 tccttggtcc gaaccaattt atttaaatag taacggcttt gatccttctt tgttccatga
    58441 tcttgaaacg ggaaaaaaat ggcttgtcaa tgaactatgg gattatcgtc tggacacacc
    58501 gaataaatcc agcggtatcg tattacaaga atatgatgca gagaagaaga agcttgatgg
    58561 tcccatctat aaaatatttg caggtacaga attagcgaaa acggaggcac cacatttgta
    58621 ttttcgtaac gggtattatt atttgatgac ggcagaaggc ggtacaggaa gaggacattc
    58681 agttacagtt tgccgagcaa agaatattac tggtccttac gagcttgatc ctggttttcc
    58741 tatgctgaca gctagtgaca aacctgcttc tacattgcaa tgtacaggac atgggagctt
    58801 agtacaaacc ccgtcaggaa gatggtacat gacatatctt tgtaccagac cattaaacgg
    58861 tgcggctatt ttgggaagag aaaccgctat tcaagaagtg tattggacgt ccgacgattg
    58921 gttaagactt aaagatagcc agacaactcc gatggaagaa ataatgattg agacaaatga
    58981 agaggtacaa caagaaaaag atcatcagtt tatggataca tttgaaggag agctaaagaa
    59041 gaactggaat gccagacgca ttttgccaaa tgctgattgg tgtgatacaa aatgtagacc
    59101 aggtttttta cgtctgattt ccggtgaatc tcttcaatct accttcgaac atcatttgtt
    59161 agctattcgg caaacggatt ttacttttga tgcggagaca gcatttgaat acatgcctaa
    59221 taattttaat cagatggctg gactggtact gtacttaaat gaatccaatt atatttattg
    59281 ttatgttact tggaatgagg acaagggaaa atgcctacgt atgatagaat cagaaaacgg
    59341 agtagcacaa atagaagatt ggacgattcc actcacagaa aagaagactt ttttgaaagt
    59401 cactgtaaag aaagagacag cacagtttca tttgaaagac gtatcaactg aagaatggaa
    59461 gaaagtaggt ccgcaaaaaa acatgtgtat actctcagga gggtttactg gaaattttat
    59521 cgggatagca gtgcatgatc taaataaaaa aagaggaagt tatgctgatt ttgagttttt
    59581 caagtatgat ggaaaagatc atctgtaatt taattcttta ataaatgagg ttcac
//
LOCUS       DACCXW010000003        55664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCXW010000003 DACCXW010000000
VERSION     DACCXW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.003"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            28..249
                     /locus_tag="IVY71_000117"
     CDS             28..249
                     /locus_tag="IVY71_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7984196.1"
                     /translation="MKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHEV
                     DMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            486..1028
                     /locus_tag="IVY71_000118"
     CDS             486..1028
                     /locus_tag="IVY71_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7984197.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1111..1662)
                     /gene="lepB"
                     /locus_tag="IVY71_000119"
     CDS             complement(1111..1662)
                     /gene="lepB"
                     /locus_tag="IVY71_000119"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7984198.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1814..2041)
                     /locus_tag="IVY71_000120"
     CDS             complement(1814..2041)
                     /locus_tag="IVY71_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7984199.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2038..2556)
                     /gene="msrA"
                     /locus_tag="IVY71_000121"
     CDS             complement(2038..2556)
                     /gene="msrA"
                     /locus_tag="IVY71_000121"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7984200.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2576..3289)
                     /locus_tag="IVY71_000122"
     CDS             complement(2576..3289)
                     /locus_tag="IVY71_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7984201.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3292..4188)
                     /locus_tag="IVY71_000123"
     CDS             complement(3292..4188)
                     /locus_tag="IVY71_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7984202.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4346..5188)
                     /locus_tag="IVY71_000124"
     CDS             complement(4346..5188)
                     /locus_tag="IVY71_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7984203.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5320..5973
                     /locus_tag="IVY71_000125"
     CDS             5320..5973
                     /locus_tag="IVY71_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7984204.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6036..6563)
                     /locus_tag="IVY71_000126"
     CDS             complement(6036..6563)
                     /locus_tag="IVY71_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7984205.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6583..7530)
                     /locus_tag="IVY71_000127"
     CDS             complement(6583..7530)
                     /locus_tag="IVY71_000127"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7984206.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7542..9428)
                     /locus_tag="IVY71_000128"
     CDS             complement(7542..9428)
                     /locus_tag="IVY71_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7984207.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(9588..11666)
                     /locus_tag="IVY71_000129"
     CDS             complement(9588..11666)
                     /locus_tag="IVY71_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7984208.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11679..11954)
                     /locus_tag="IVY71_000130"
     CDS             complement(11679..11954)
                     /locus_tag="IVY71_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984209.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12316..12771)
                     /locus_tag="IVY71_000131"
     CDS             complement(12316..12771)
                     /locus_tag="IVY71_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984210.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(12925..14133)
                     /locus_tag="IVY71_000132"
     CDS             complement(12925..14133)
                     /locus_tag="IVY71_000132"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7984211.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14245..14577)
                     /locus_tag="IVY71_000133"
     CDS             complement(14245..14577)
                     /locus_tag="IVY71_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7984212.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14725..15600
                     /locus_tag="IVY71_000134"
     CDS             14725..15600
                     /locus_tag="IVY71_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7984213.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15682..16263)
                     /locus_tag="IVY71_000135"
     CDS             complement(15682..16263)
                     /locus_tag="IVY71_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7984214.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16269..17528)
                     /locus_tag="IVY71_000136"
     CDS             complement(16269..17528)
                     /locus_tag="IVY71_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7984215.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17530..18576)
                     /locus_tag="IVY71_000137"
     CDS             complement(17530..18576)
                     /locus_tag="IVY71_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984216.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18811..19086)
                     /locus_tag="IVY71_000138"
     CDS             complement(18811..19086)
                     /locus_tag="IVY71_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7984217.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19329..20639)
                     /locus_tag="IVY71_000139"
     CDS             complement(19329..20639)
                     /locus_tag="IVY71_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7984218.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(20821..22050)
                     /gene="rpsA"
                     /locus_tag="IVY71_000140"
     CDS             complement(20821..22050)
                     /gene="rpsA"
                     /locus_tag="IVY71_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7984219.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22185..22865)
                     /locus_tag="IVY71_000141"
     CDS             complement(22185..22865)
                     /locus_tag="IVY71_000141"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7984220.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(22914..23549)
                     /locus_tag="IVY71_000142"
     CDS             complement(22914..23549)
                     /locus_tag="IVY71_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7984221.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23615..25018)
                     /locus_tag="IVY71_000143"
     CDS             complement(23615..25018)
                     /locus_tag="IVY71_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7984222.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(25005..26036)
                     /locus_tag="IVY71_000144"
     CDS             complement(25005..26036)
                     /locus_tag="IVY71_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984223.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26067..26288
                     /locus_tag="IVY71_000145"
     CDS             26067..26288
                     /locus_tag="IVY71_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7984224.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26453..27190)
                     /locus_tag="IVY71_000146"
     CDS             complement(26453..27190)
                     /locus_tag="IVY71_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7984225.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27343..28683)
                     /locus_tag="IVY71_000147"
     CDS             complement(27343..28683)
                     /locus_tag="IVY71_000147"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7984226.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(28920..30443)
                     /locus_tag="IVY71_000148"
     CDS             complement(28920..30443)
                     /locus_tag="IVY71_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7984227.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(30821..31432)
                     /locus_tag="IVY71_000149"
     CDS             complement(30821..31432)
                     /locus_tag="IVY71_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7984228.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31555..31680)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(31762..32478)
                     /locus_tag="IVY71_000150"
     CDS             complement(31762..32478)
                     /locus_tag="IVY71_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7984229.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32484..33053)
                     /gene="scpB"
                     /locus_tag="IVY71_000151"
     CDS             complement(32484..33053)
                     /gene="scpB"
                     /locus_tag="IVY71_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7984230.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33037..33834)
                     /locus_tag="IVY71_000152"
     CDS             complement(33037..33834)
                     /locus_tag="IVY71_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7984231.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(33804..34175)
                     /locus_tag="IVY71_000153"
     CDS             complement(33804..34175)
                     /locus_tag="IVY71_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7984232.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34194..35081)
                     /gene="xerD"
                     /locus_tag="IVY71_000154"
     CDS             complement(34194..35081)
                     /gene="xerD"
                     /locus_tag="IVY71_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7984233.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35177..35650)
                     /locus_tag="IVY71_000155"
     CDS             complement(35177..35650)
                     /locus_tag="IVY71_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7984234.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(35755..36618)
                     /locus_tag="IVY71_000156"
     CDS             complement(35755..36618)
                     /locus_tag="IVY71_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7984235.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(36814..38913)
                     /locus_tag="IVY71_000157"
     CDS             complement(36814..38913)
                     /locus_tag="IVY71_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7984236.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(39133..40242)
                     /gene="rpoD"
                     /locus_tag="IVY71_000158"
     CDS             complement(39133..40242)
                     /gene="rpoD"
                     /locus_tag="IVY71_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7984237.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(40266..42107)
                     /locus_tag="IVY71_000159"
     CDS             complement(40266..42107)
                     /locus_tag="IVY71_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7984238.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(42289..44682)
                     /locus_tag="IVY71_000160"
     CDS             complement(42289..44682)
                     /locus_tag="IVY71_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7984239.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(44789..45649)
                     /locus_tag="IVY71_000161"
     CDS             complement(44789..45649)
                     /locus_tag="IVY71_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7984240.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(45708..46229)
                     /locus_tag="IVY71_000162"
     CDS             complement(45708..46229)
                     /locus_tag="IVY71_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP7984241.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            46549..47268
                     /locus_tag="IVY71_000163"
     CDS             46549..47268
                     /locus_tag="IVY71_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7984242.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            47294..49885
                     /gene="mprF"
                     /locus_tag="IVY71_000164"
     CDS             47294..49885
                     /gene="mprF"
                     /locus_tag="IVY71_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7984243.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(50073..51260)
                     /gene="tuf"
                     /locus_tag="IVY71_000165"
     CDS             complement(50073..51260)
                     /gene="tuf"
                     /locus_tag="IVY71_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7984244.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            51628..51984
                     /locus_tag="IVY71_000166"
     CDS             51628..51984
                     /locus_tag="IVY71_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7984245.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(52225..53229)
                     /locus_tag="IVY71_000167"
     CDS             complement(52225..53229)
                     /locus_tag="IVY71_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7984246.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            53395..54246
                     /locus_tag="IVY71_000168"
     CDS             53395..54246
                     /locus_tag="IVY71_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7984247.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            54263..54508
                     /locus_tag="IVY71_000169"
     CDS             54263..54508
                     /locus_tag="IVY71_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984248.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            54695..55399
                     /locus_tag="IVY71_000170"
     CDS             54695..55399
                     /locus_tag="IVY71_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984249.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        15669 a        11573 c         9551 g        18871 t
ORIGIN      
        1 ctctctcatg atttttataa aaaatttata aaagataaag aaatagctga taataaaaaa
       61 acaagtggtg ctttgatttc gtttagtgtt gaaaatgcgg atgaagtaaa aaaatttgct
      121 caaattgcaa aagaaaatgg tggtactttc cacgaagtag atatgggaat gcctgaagaa
      181 gaaatgtttt cattagaagt ccaagatcta gacggaaata cattagaacc cgtatggatg
      241 aagatgtaag cagataacta atctgacgtt ttttataaca aaaaaacaaa ggcggatgat
      301 acgataagta ttctccgcct ctattttttt tattcgtaca atctcttttt tacagtttat
      361 aaaagcagtc ttctcaccct ttttttaatt gttagatgcc agtgtatgta aatcaccaat
      421 gtgtcatgac acattggtga ttttatggta tccttttatt attaactgac aaaggagaga
      481 atattttgaa cactttgatt gcaaaagacg tttttagatt gcccgaaatg aatgcaagat
      541 tgttgtcgac ctctgctctt ttattagccc ttgatgtcat cttatacaag atagcgattg
      601 gtcctgctta ttttcaaatc acattcggat ttttaagtat ggcgcttatg ggttatctat
      661 atggaccgat ctgggctggt ttgcttgaaa tgttgtcgaa cactttaaat ttcacgatat
      721 ttggttcagg aacgtttcaa gctgcttttc ttatcacggc tttccttggt ggatttatta
      781 atggcttatt tttatatcat aaaaagataa acctgccttc cgtcatcgct gcccaattat
      841 tgattatgat cccagtcagc ctttttatca attcttggct gatgtctgtt ttgtttggtg
      901 cagatttcaa agtaattttt ctaggtagat tcatcaggaa tatgatccaa atccctattc
      961 aaataatggt tgtttattca tttatcaaaa ctttagaggc tagaaaagtg tttaaacgag
     1021 ctgtttgatt ttacgtacta aggtagtaaa ctgattttgg attaaacaat aaagcgtgca
     1081 gtctcaacag actgcacgct ttattgtttc tcactggatc cattttattt catcaagtgg
     1141 ataatagaca aatcttgctt tacctagaat atcagtttta ttgatcgtgc caaacgaacg
     1201 actatctttt gaaactcttc gattatctcc aagtacaaaa taattatctt ccggaagctt
     1261 ggattgtccc gttagctctt gtagtgtaaa gtccgtcgta taggatgcat ttgcatgatc
     1321 tgatttcagg tttttagtta gataaggttc cttaactact ttcccattga tcttcaattg
     1381 atcattttgg tagctgacat tttctcctgg aagaccgatt actcgtttga tataaatcgt
     1441 gccatctgct aattgaaaaa caacaatatc aaatcgtctg atctccgaaa atttttccat
     1501 cacaaccata tcaccttgct tcaacacgtt ttccattgaa ttgccttcca ctggtaccgg
     1561 gatgaataaa aatccccgca acatgaacgc aaggaataca gaaacaagta agtatttgaa
     1621 tacaagccag aagcgattaa tatagcgttg tttttttgtc aatctctatc aatccatccc
     1681 tctttttttc attctttctt tattataaga aaaatatcaa aaaaatgcga ctagatagct
     1741 gaaataaaaa taattctttc aattaggcta ttggacaaaa aacttttttg atcataaagt
     1801 acaaaactat ctcttatatg tcatgattat tttctaaata tttttcccat gcttcgtcaa
     1861 agatatccat ggacggcaaa tagtcgacat tcagttccag ataattagaa atttcttcgt
     1921 agctagagct gtgcttagga aattggatgt ctttaccggc ttctgtagcg aaaacagata
     1981 aagcatcatg tgtgccaccg cctcgcagtg tcattaaata atgaaaaaag cttcgattca
     2041 cccttcgttc ctccaatttt cttcgataaa ggcagcccgg cgtgcatggt ttcttttaaa
     2101 attttcagga tgctttttat aatactcttg atggtcttct tctgctagat aaaatggttg
     2161 ggctggttcg atttttgtaa cgattggctg ttcgaaacgt ccactttcag ctaactgttt
     2221 cttacttttt tcagcaatga tccgctgttc ttctgtcaaa taaaagatca ccgggcgata
     2281 attgtcaccg cgatcctcga attgtccgaa agcgtcagtt ggatcagttt gctgccagta
     2341 aagttctact aattgttcat atgaaatcaa tgttggatca tagataatct ccactgcttc
     2401 tgtatgtcca gtcgtttggc tttttacttg ttcataagtc ggatctggta catgccctcc
     2461 agtatatcct gacatcactg tatggatacc aggaagcgtg tcgaaaggct ggatcataca
     2521 ccagaagcag cctcctgcaa atattgctcg ttccattatg attccccctc taatactact
     2581 catccgatga ttttttattt tcttttgtct tgctttcagt actgctggtg ctagttttgt
     2641 tggtatttga gttgtttgaa gcatcagagg tatcttttgg caaatataga ctaaaccgaa
     2701 tgtcatcatt tactaagtcg attttatctg ctttaatata catcccgttt tgcattcgga
     2761 attgatccaa cctaagcatg atagtcagat catctgtatt gacttcgatc caatctggta
     2821 atttatagtt tctcttgatc atattcatca catctttgat tggcaaactc aatgttccga
     2881 tagaaagact tctagctttt agttggacat ttccgttttc catcacatac ggatcaaaat
     2941 ataaatagaa atcgatagga aaacctagta cttcgaaagt accattgagt agtgcttcat
     3001 tttccaaata aaatttatat tcaatatcac tatctttttg atactcacta aggaaaaagc
     3061 tgatgatttg attgactttt tctttatttg aattgatcgt taagacagga tctccttgtc
     3121 tttccgtaag agctggtatt tctgggagtt ctggttcccg attagcaaaa atacggctgc
     3181 ctaaaaatac ggtcgaacca agtactaccc caagaaggat aaggaaggcg aatttccagt
     3241 aatttgtttt ctttgttttg tttgaagata cgtcagatct tgttttcatg tttattgatc
     3301 atcctccttc tctaaccatt ggttttgtgt ctcgatcagc tgatctctca ctgcatttgc
     3361 catcaattga taacctaaat tatttggatg gaaatgatct tcttcataga gcacattatt
     3421 tttgatgcca tttagatttt ctgtcgtgct agttgtttga tctgttcctt cttctgtgac
     3481 acctacttcc tgattcaatc cttgataaag caaatcattg attgggataa agtagcagtt
     3541 cggcattttt tttgtcaccg tttctgttcc tttgttccaa ttgttaacaa ttgtctgcat
     3601 gtcagtgatc tcaggaaagt tcaaataaaa tggattgtag atgcctaaaa tgtaaatagg
     3661 tgcggtttta ttgagggatc gaaattcttc caatagttct gtgacatttt cctgatagtt
     3721 tttcagtggc tttttaaagg aattgaccgt taacccgaat aaattatttt ggataacttt
     3781 catcaaatca ttaccgccaa cagtcagtgt gataaaatca gctgttttga tattgtttcg
     3841 aatttcttct tttgttttta ctcgctttaa gatctggtca cttcgttcac catttacacc
     3901 atagttttct gtttcaaccg cggtcaaatt ataacggtct tttagatcat ttgcgacaag
     3961 gggaacgaat cctccttgct tggtttgatc tcctactcct tcagtcagcg aatctccgat
     4021 agctatgtag tgaagttttt cttttttatt tggatcttct ctcacagtag ttactggttt
     4081 cattagtggg ccagctttcg gtatagaagc agctaaacct gcatagatga tgatcgcaat
     4141 taataatggg aacaaaacga ggatcaaatg tcgtttcgtt aatttcattt tttcacctct
     4201 tgtattcgat tggttctaat gttatcaaaa attttatcaa aaaggtagtc tttatgaaaa
     4261 aagaaaacaa ttattttcct aattttataa agaaaaagag aataagctgc aagcctttca
     4321 ggctgagggc ttatcctctt ttcgactaat ctacataata catgattgca aacgcgtctt
     4381 taccagtatg cgtagcgata accggattcg tatgcagtac agggatatgc atgtccggga
     4441 ataattcttg caaaccctct ttgaattctt gcgccaattc ttttgcatcc gcatgtgaaa
     4501 taccgatttg tcgcacattt ggcaattctt tcagttcatt tttcaaatta ttaaaccatt
     4561 tatcgaacgt tttctttcct cgccccttag ttacagggat taattcagaa tgggcgaaat
     4621 tcatcaccac gcgtatgttc aaaacactag acagcaaacc agttgttcgg ctgattcttc
     4681 caccttttac caagttatct agtgtggata ttccgatgta taattttgta ttgtcttgta
     4741 cttttttgac ttcttcaagg attttctcta ctgttttacc ttcttttgct aattcagctg
     4801 ctcgaattac ctgaaaagat aatccttggt cagtggtatc actgtcgata accgttactt
     4861 tcgtttttgt tagattactg gcttgtcttg ctgcttctac tgttccactc aagccttttg
     4921 tcatatggat cgaaatcact tcactgccgt cttctcctag ctcatcatac atcgagatga
     4981 attctccaat tggtggttgg cttgttttgg gcagattttt tgctttcgcc atcatttcca
     5041 taaatctttc gccgtctagt tggtcatcat cgggatatac tgttccgtcg atcatgatgg
     5101 atagcggcat gacacgaatg cctaaagcat cgcgtacatt tttttccatc gtacatgaag
     5161 aatctgttac gatttttaca tttgtcatta ttttctctct ctctttcttt aacaagatgc
     5221 cttattcatg ttaaactaca tgtaagacaa tttctatttt tagtataaca gacaaataat
     5281 gatttttcat taattacacc tagtaaagga gccgttttca tggaaaaaac gaaatttaat
     5341 cgaacgtatg aaatattaaa tgaagtgttt aatgcagtca cccatggaat tgggactgga
     5401 ttgagtatcg cgggactagt ggttctttta gtaaaaggtg cccacttgca ttcctccctc
     5461 catattgtct cttatgctat ctatggatcg atgatgattc ttttattttt aacgtctact
     5521 ttgtttcatt cattgatttt tacaaaagca aaaaaggtgt tccaagtatt cgatcatagt
     5581 tcgatttttc ttttgattgc aggaagttat actccttttt gtttgctgag tatccgagga
     5641 tggctaggtt ggactttatt tgtattaatc tggttgtttg cgattcttgg cattatatat
     5701 aaagcgttga cgttgcataa acgggagaga gtatcaaaag tatctacgat catctatatt
     5761 tttatggggt ggctctgtgt cgtggctgct gttcctcttt atcactctct tggaatgact
     5821 ggtgttattt tgatggtagc aggcgggctt tcttacacga taggcgcatt cttttatagc
     5881 ttgaattcta tccgctatat gcatgttgtt tggcatttgt ttgttatgtt aggtgcaggt
     5941 tttatgtact tctctgtttt attctttact taataaaaaa aataaagcta tggatgggac
     6001 agctctaaat aacagctagt ctatccatag cttttttata tcgtcaattc gcctgtcccg
     6061 ctttttacag gattctcatt acgctgatac gtttcaaatt gatgcggata acgatttttt
     6121 tcatccattt ctccttgact agtattgatc agtgaccaat cagaccagtc aacaggtgga
     6181 aaataagcat ctccatcgaa tacatgatgg atgactgtac ggtataatat cgtacaaaat
     6241 ggcagaaatt cttgataaac agctgaccct ccagcgatga aaaagatacc gtctgtttct
     6301 ttttccagag caagcacgtc ttccacacta tgcatgacat gcacgccttc tgctttatag
     6361 gaagaatctc tcgtcaaaac aatcgtctga cgattcggca acggacggct acccatccct
     6421 tcgaatgttt ttcgtcccat cactagtgta tttgcttctg tcatttgttt gaagaatttc
     6481 agatcatttg gcaaacgcca aggaagacgg tcttcttttc caatcaatcc gttttcatcc
     6541 tgtgcccaaa ttgcagctaa cattttctca tcctttctga ttctaaacag cgataggagc
     6601 tttgatcgcc ggatgtgggt catatccttc taattggata tcttccatct caaaatcaaa
     6661 tactgactgc ttttcctgat tcaaaactaa agtcggaaat gcgcgtattt ctcggctcaa
     6721 ttgttctttc atttgttcga tatgattgat ataaagatga gcatctccta atgtgtgaac
     6781 aaattcgcca accgctaaac ctgtttcatg tgcaattagg tgagtcagca acgcataact
     6841 tgcaatgttg aacggaacgc ctagaaacac atccccactg cgttgataca attggcagct
     6901 tagccgtccc tcttgaacat aaaattgaaa catcgtatga caaggaggca aagccatgct
     6961 aggaacatct tccggattcc atgcagaaac gatcaatctt ctagaatcag gagtcttttt
     7021 gattgcttcg atcacttgtt tgatttgatc gataaagcca ccgtcttttg tttcccagtg
     7081 acgccattga tagccgtaga tgtgtccaag atcgccgtat gtttcagcaa aggaatcatc
     7141 ggttaggatc ttctcgcaaa aaagcttgct ctgcttttta tattcttcat tgaattcagg
     7201 atctgtcaaa catctatggc caaaatcagt catatctggt ccattatagt ctggactttt
     7261 gatgtagcgt tcaaatgccc attcatccca aatatggttt ttattttgta atagatagcg
     7321 gatattcgta tctcctttta aaaaccaaag caattcgctt ttgatcaatc caaaaggtac
     7381 tcgtttcgtt gttaacaaag gaaacccttt ttgcagatca aaccgcattt ggtatccgaa
     7441 taagcttaat gtgcctgtgc ctgttctgtc ttctttacga tgcccttctt ccaacaattt
     7501 tcttcctaag tcaagatacg cttgttccat cctttttcct cctaatcttc tacccattca
     7561 ctcaagtatt cccaccgtgc cattttttct tcaagttgtg cttctgtttc agatacgtcg
     7621 ttttgcaatt cttgtaatcg agtgaaatta tctccttgat gattcatctc ttcttggagc
     7681 aatgagattt tttcttcaag ttctgcaatc tcatcttcaa tcgtttccca ttctttttgt
     7741 tccatgtaag ataatttctt cttttcagca ggttccttgc tctgtatttt tggtttcgct
     7801 ttttcagcag gctgctgggt ctctttttgc ttcagtaaat aactggacat gtttccgtaa
     7861 taaagttctt gttttccgtt tccttggaat actaataact tatctgctac cttgtccaag
     7921 aaataacggt catgggaaac agtgatcaca gcacctggaa aacttctaaa ataatcttcc
     7981 aaaatcgtca atgttgctat gtccaaatca ttggtcggtt catccaataa taaaacatta
     8041 ggttgctgaa tcagcagttt caacaaaaat aaccggcgtt tttctccgcc tgaaagtttg
     8101 cctatcagtg tcccatgcat aaatcttgga aataaaaatc gttcaagtaa ttctgctact
     8161 ccgatttgac tgccatctgc tgtttttgct tcttcagcag cttcttgtaa gtaagcaatc
     8221 atccgtttat ctggtgccat ttcttcattt tcttgtgtat aatacgcaag atgaaccgtt
     8281 tcaccgatag acattgtacc gctatccagg ggaattctgc cagctagaat attaagcaat
     8341 gtcgattttc ccgcaccatt tttaccagta atacctagtc tttcccgaga ttgaatcaat
     8401 aaatctaaat gttctagcag tgtttggtca tttatacggt aactgccatc tttgatttcc
     8461 agtacttttt tccctaaacg ttgtgtcgct aggttcagct ctaaatcatc ttcttggttt
     8521 acttggtaca agttctcctt tagatcttcg aaacggttga tccgtgcttg ctgcttggtg
     8581 ccacgtgcct gtacgccagc cctcatccaa gctaattcct gtttataaag ccgcttgcgc
     8641 ttttcttctt gttctacttc tacacgatca cgttctgctt tttctaagac gtaagcttca
     8701 taatttcctt tatactcgta taattttcca aatgaaagtt cgaagattcg gtttgctact
     8761 ctatctagaa aataacgatc gtgggtaacc atcaatagtg cgccacggta ttgtttcaaa
     8821 tagttttcca accactcgat cgcttcataa tctaaatggt ttgttggttc gtctagtaat
     8881 agtaaatcgg gttcatcaat caacacttgc gccagactaa ctcgtttttt ctgaccacct
     8941 gataaagtac tgattttttt atctaattca ctgattccta atttttgcaa aataatcttt
     9001 gcgttggtat ctgtagtcca agcatcttct ttattcattt gttcttctgc tttagcataa
     9061 cgtttttgca catcagggtc ttctccgttt tcccctagtt cgatcaatgc ttcctcatat
     9121 gctcgaacag tttggatcaa ggggctgttt ccttgaaaga ctgcttggag aacagttaat
     9181 tcatcggaaa attcagctgc ttgagaaaga taaccaatac ggtaatcagt tggataaaac
     9241 actgtttgcc gatcaccgtc gctgctatcg ataccggcaa taatatttaa gagacttgtt
     9301 ttacccgtcc cgttcgtccc aatcaacccg atacggtctt tttcgtggac caaaaaagaa
     9361 atctggtcaa acaaatcttt ttcaccataa gtttttttga ggtcagttac ttttaattct
     9421 ttcaacatgt tccctcctta tcttggatat tgtatcataa aaatagtgaa cttcaggaaa
     9481 atttttttgg tatttgttct ttttgtgttc tcaaaaaaag aacaggaccg attcgatcct
     9541 gttctgatat gttttgtttt ttataaaact tgaaaatttt taccttttta ttttattgga
     9601 ctcgtttttt ttgactttgc tcgaaacgca aatcggaaag aaaagttatc agatggtttg
     9661 ttcatcgtat aagattctaa caaattgata ttcctgcgat agctgttcgc agaaaacgtc
     9721 gtgatttttt cagcaacttc gtattcatca atgatttttc tttcaagata ataacctctt
     9781 agcatttttt tcgtaaacag actatcttgc tggatcatcc acgaagaaat caaattcccc
     9841 gagttcattt ttattccttc aagtttgcgt tgctggataa aaaattgaac catcgtttca
     9901 tcataaatat cagtcaagtt atctaaactt tcaataatca actgagtatt tttttgatat
     9961 gtctctctga tttcttggat atcttcatca ctaataaatg tctgacgatc agcatatctt
    10021 ctttgccaaa acattttcgg atggagaacg attcgcatta acctacggac aacaatgaac
    10081 caaaagccga taaaagacaa aatctggctt gtgatagaat gttcgaatcg agaaagaaaa
    10141 cgggagtaaa aacgataacc attagaagaa agcttgcccg aacgataact ttcatccaat
    10201 ccatcccgtt cgatcgacaa aatcagtgct tgtatttctt gtacttcttg cttttcatca
    10261 tcggttaaat cttccaagta cagatcacgt atccgttctt gataattttc tatcactgct
    10321 tcagtggcta aaaattcttt tgtattacga tcaatttcct ttatttcttt tcgcaaaact
    10381 tcaacgacct cttctaaaat catcaaagca ttcaaatccg tgcttttcgc ttcctctgtt
    10441 tccgtcagca taggcaagac aatgatccct atgaccaaag tcactaagat gacacccgcc
    10501 gtcagaaaca aaagcaaaga ccgctggtaa aacatcatat tattgattga aaatggcaaa
    10561 ataaaaatcg tagccaaact gactgttccc tttacaccgc caaatgttaa gatcaagatc
    10621 tcgttcaact gttttttgaa ctttttactg ccatctttga atacataaaa aagactgata
    10681 gaaatgaatc tgatcacaaa aagcatgaca gagattaata cgataatcgc taataaaaga
    10741 ccgtttggat ataaaccgtc tccccatact ggcgagaaca cttgcgtcaa ttcgattcct
    10801 aaaaatataa atactaatgc attcagtgta aaagcaatag ttgtccatgt actgtgggac
    10861 aagctcgaca attctgcatc aaacacagag attttcctaa aaccagaagc ttggagaata
    10921 cctgctgcca ctgcagcaat gattccagat acatcaaatg cctctgccaa tacataggct
    10981 agaaaaggaa gcaaaagttc gatcagcaga taccctgtga catcttgtgc tgatgctttt
    11041 tcgatcaaat gaattatttt ttgtttgaac cagacaagca aaaagccgat caaagctccc
    11101 ccaatactag aaactactag actcatcccc gcgtttactg ctgaaaaaga tccggtgatc
    11161 agcgcaccaa gagcaaactg gaaggcagta acaccagatg catcattcaa aagaccttct
    11221 ccttccaaaa tatgcatcgc ctttttaggg atattcactc gtccagaaag agaactgact
    11281 gctaccgcat ctgtcggacc taacgctgct ccaaaagcaa agcaggcagc aagtgggaca
    11341 cttggcaaca ggaatgataa cgtcgcacct accccgacaa gggtcaaaat gactcctcca
    11401 aatgccaaga acaaaattgt cccgaaattt ttgagaatcg aagaaatatc tgctttttct
    11461 ccttctctaa ataacaatgg tgcaataatc ataactaaga acaattcagg ttcaaaatcg
    11521 attgattgtc cccactctgt cagacctaat accactccaa ggaaaatttg gatcagtgga
    11581 gctggaatca ttggcaaaat gcggctagct atgttcgata aggtgatcgt tactgcaaat
    11641 acaataacta actcagcaaa ttccatcgtc catccccttt attttttatt ttgtgtaagc
    11701 atttttaaaa attgttcttt tttctttaaa attttggttt cgactacttc aacctgtgtt
    11761 tggttcccct ctaattcaca ctgtacacgt tttgactcca aacgaagcag atcatgtttc
    11821 agacgttcca actgacgata catcaatata tttcttttac gcaacttaca aagtatttga
    11881 cggcggtcat ccatcgaaag ggcgccatag cgatcatgca acagtttttt ttgttgttca
    11941 aaactctctc tcattttcca gatctcctat acacaaactt ccatcattat agcaaagttc
    12001 tttttggatg aaaaatattt cattgtttca tcgtttattg ctagttagaa atttccataa
    12061 atttttcaac gcctcattct attccaaata gtatcagata tcaagtaaaa cttaagaaag
    12121 ataaaagttt ctgacaaaag gatgtttact ttcccattcc tgcctttctt gttcttttat
    12181 cacaacaaaa aaatggggct gcgacaaaaa tcttgtcaca gtcccttatt ctgaataaac
    12241 ggtgtggaag agacagccta tcagtagtaa taagacacat ctctttcaca gcctcatttt
    12301 tgcgtaaaaa tcgatttatt cgaaaaattc cccgtataca tcttctacac gttgatctgg
    12361 attgatcacg atatacaagt tacctgtttt ttcgctgatt tttgttgatt gatattgatt
    12421 gtctaatcct tctgttcctt tatctagata tgggaagtac acaagatcag gtgtgccatt
    12481 tcggtaaagg atttccattc gctcgatatc ttcgtatttc aatgcgcgat taaacatgcc
    12541 catttccagt ccgcctaggt tgatatcatg tgtcgcaact gcgtcccctt catggaagat
    12601 ttcgatttta aatccagcac acggatggat ttcaacaaat tcactgccat tgattcgtcc
    12661 aaaacttgtt gtgatttgtt tgatccaaag atcgccgata tgacggcggt tgatcgtcca
    12721 ggtttctcca tttctaaaat aaaattttag cgctgtcatt tctttactca ttaaaaagac
    12781 tcctttatga tgtaatttat tcttgtacta cctcagttta gcacaaattt atgtaagtgg
    12841 tcacatctta tgttttttct tctaatgacg tatctgccca ctttaagtag ccaacaaagc
    12901 tgagcagata cagtctagtg ttttttaaat caatttttct ttcgataaaa aatccaaaac
    12961 ctgtttttta tcatttttta ttctttgttc tacgacggct ttttcagcta attgcaaagc
    13021 ttcactaacc cacttaccag gtttttttcc agtttcttct agcaagattt taccatctac
    13081 tgctaattcg cttaatgact tgataggtaa acgatcatag gattctccta cggcatccat
    13141 ctgaggtgtt tcgtcaaaat agatcatcaa ttcttctact tgcagtgcat gttttttgcc
    13201 taagaagtac aattcgaatg gttccaaagc tccagtttct cgttgtcgta gtccttggac
    13261 aagtgcttga acgtctttaa tcatttggtt agaacacttc cacgatttca aaaaggaacg
    13321 aatctcattt tcttttagat ccagttggct gatcaataat gcccatgctt ggctctcttc
    13381 tttcaatggg acggatggta attcagcaaa acgtaaaagt gcttcgcctt cttctttcaa
    13441 accgggacag caaatgtagc actctgtttc aataaatgca tggattcccg cggcccgttc
    13501 atttcccaag agcatcttga tgaattccac atttatacgt tctacagata ttttacctaa
    13561 tagctcgtgg ttttctttga tggcgtagaa ggtttcttcc tctaatttga accctagttg
    13621 actgacaaaa cgcaacccgc gcatcattcg taaggcatct tcatggaacc gctcatgagg
    13681 attgcctaca gctcgcaaga tctgttcttc tagatcttgc agaccatcga acaaatcgat
    13741 gatttctcca tcttctttca aagcaaacgc attgatcgta aaatctcttc ttttcaaatc
    13801 ttctgccaac gaacgaacga attcgacatg atctggtcta cggtagtcct gataagttga
    13861 ttccgtccga aatgtggtga tctcatattg ttcatcattt tctaatacca acacagttcc
    13921 atgttcgatc ccaatatcta tcgtttttgg aaaaagctgt ttgatttctt ctggaaatgc
    13981 gcttgttgcg atatccacat catggattgg ctggttcagc aagatatcac gaacacttcc
    14041 acctacaaaa taagcttcat atcctgcatt ctctaatttt ttcagaactg gaattgcctt
    14101 ttggtattcc agaggtaatt gttttaattt catttttcca acctttcctg cgtgtttctt
    14161 gtcctgagat aaagactcag ttgcatggtg actgtttgac aagtccgttt cagagtcttc
    14221 aaggccagga ccacttcatc ttgtttactc gtccttacgc tcaaagcgat tatggtctct
    14281 ttgattgaat ttttccatgt ttttttcaaa aacttcagtt aagtcgatat tcaatgaatt
    14341 agccatgatc atcgtaacga ataaaacatc accaagttct tctgctaccg agttaactgg
    14401 ttcagaagcc tttttgcttt tttctccata agtgtggttg acttctcttg ccaattcacc
    14461 tacttcttca gttaatcgag ccatctgagc taatggagag aaatacccca ccttgaattg
    14521 ctggatatat tcatccactt cctgctgcat actgtataaa gaacgctttt tatccatctt
    14581 tttccctcca aaactatctt accaaaactt taacgcaaat cctagataag agcaattgag
    14641 aaaatcgaat ttccatgcta catttaggaa ggaatttcta aatggaaatt ctgtgaaaat
    14701 acataattga aagctggaat tcatatggtc aaacgaaaaa cagtaaaaga agttcttttt
    14761 atcattatag gaacaagtat ttatgcattt ggtcttgttt acttgaacat cgctaatcat
    14821 ttagcagaag ggggagtctc agggatcacc ttgatcttaa gagctttatt tggtattgat
    14881 ccagcttata cgactttatt aatcaacatc ccattgattt tactaggagg aaagatcctc
    14941 ggtaaacgtt ccttggccta tactgttcta ggtacacttt ctttgtcatt ctttttatgg
    15001 atctggcaaa gggttccact agaaatcaat ttacagcatg atctactgat cgtttctcta
    15061 cttgcgggtt tagttgcagg tgtaggaagc gggatcgtct accgcatggg tggaacaaca
    15121 ggcggcagtg atattatcgc tcggatttta gaacaaaatt acggcatcac tatggggcgt
    15181 tccctattgg catttgatat catcgtctta cttgcttcat taacctatat cgacttgaaa
    15241 cgtatgatgt atacgttgat tgtttcgtat gttttcagtc gtgtgattga ttcgatctta
    15301 gatggcggat attctgcaaa aggtctactt gtcgtctcca acaaaagcga agagattgca
    15361 ccactactga tgaccggtct gcaaagaggt gtgactttcc tttctggaga aggcgcattc
    15421 tctggtaatt caaaaaaaat catctatatg gtcgttagct ctagtgaatt aactgaagct
    15481 aagaggatcg tccacgagat tgacgaaaaa gctttccttt caatcatcaa tgtgcatgaa
    15541 gtggaagggc aaggctttac ttatttgaag ccacagacaa gattactgaa aaaactataa
    15601 gaaaaaagac aaagataggc tatgaggaaa gattccacat ccagctgacc tttgtctttt
    15661 ttagtactat actattttta actaattgtt atgttcactt acccggttac ttttcttttg
    15721 tgtctgcaaa aggatctttc ggttaacttc atcagataat ttcaaaaaag catccctatc
    15781 tccatcttct agtgcttgat cgatttgagc ataaagcatt tttagttttg cctcttcttc
    15841 tttttcttga aaatatcgtt caattaattc agtatcttct tcactgactg aagaatccca
    15901 agaagaaaat ggattgtctt ctaagataga tagatattct cttgtctgcc aagcattttc
    15961 gaatatacat tccacatata aaggcttttt ccaattcaaa cggatttcat gaaaaatctg
    16021 gtctgtgtcc gtaaattctt ttccctgcaa aaacaatttc atgggttccc cttcaacaga
    16081 tagatcactg atctgcagtc ctcgatccgc aaaattcgcc ccttcaacaa agtggacatt
    16141 gcttaaaatc gcttcatgat tggcaagata attcaagatc caaaaaactt ctcttcggct
    16201 aaaagataca tgattgacca tccacgttaa aaaattcttc ttttcccgta catcaattag
    16261 catttttctc acctctctgt taggtcatct aataatgaca gtgcctccat atctcctggt
    16321 tcaagtgtta aataataagt caataattct tgcgctcttt tcagctgccc ttcttctctt
    16381 aagaaaaagg catactcttt caagaaatct ggttcatgtg atagttcatg ataagcctgt
    16441 tcatagtgta cagctgcaac ggtaaagtct tctaattcat tataagcatg ggctaggttc
    16501 cattctgcat aaggattagc agtttcttcc atctgattga tcacttgtat cacatcttca
    16561 tatctttcat catccaagta aagattactt aaagtaagta atgtttcatc ttgtttgtct
    16621 cctacttcta aagctttcag taaaagttct tctgctcgct gtctatcatg cagacggaaa
    16681 gcattttccg aggccaggtg ataaagttcg acttggtaag gattttcttt gattccttct
    16741 tctaacactg tttgtgcttc ttcaatcatt tcttcttctt gaagagcttg tcctagatat
    16801 agatataacg attgataatg cggattaact tctcgaagtt cttccagata gttgattgcc
    16861 tgctgatttt ctttcaattg aagattcgta aaagctagct ggaacagtcg atcatctgtc
    16921 ctttcttctt tcagtgcttc ttctaaaggt acgatagctg cttcgaattc tccttgcata
    16981 cttaaggatt gtcctaaacg ttcctgcatc gagattcctg aaatttcatt tatcccgctt
    17041 gccaacaatg tttcataaac cactgctgcc tctttgaaac gatcaactga aaaatacaat
    17101 tcagctagcg caaactggat caatggttca tcaggaagca acccagctgc ttctttcaat
    17161 tttgcttcac tcacttcagg gattccgata acttggtaca aatcagctat cgccaataag
    17221 ctttgcggat agtaatcact ttgcttatcc actttttcca ggtattcaaa agctaagtcg
    17281 atctcattat cttcgattgc aatttctgct aacgggatgt tcagtccgtc attttctggg
    17341 tattggttga tcagttgttc aaaaatctgt tttgcttcga tcaaaaagcc aatagccagc
    17401 aattcttcgc ctagttcagc taatatatct gctgggtctt cttttaatgc tttttctaaa
    17461 aagatttgtg cttgtgacaa gtcttcatct gataaagctt gcaacatttt ttcgctattt
    17521 gtattcattt caattccctc ttatctcact gacgatttgt tgaaataccg tcattgttct
    17581 caataattct ttttcgtttg gatgttggta tccgtctatt atacctattt tttctaaaac
    17641 aagtattttt ttgctttttt ctgccaaaaa aagaactttt tccaaatact ccatctttga
    17701 aatctgttcc agccagtgga ttggatagcc aagcaagtcc agccacacaa aaaagttttt
    17761 tagttggaaa tcaaaaggat atgcttggag attccacacg aaatgaaaca tcattcctaa
    17821 aaattttttc atactagcag aaagtagatg gccattttcg gtcagataaa aagcttgttc
    17881 aaacagttta ccataatttg acaggttttc tgcctctaat tgataaaaat agatcatttc
    17941 ttcgatcatc ccggcaaacg gccgagtcgt taattgcttt ttatttggat agtttttata
    18001 cagattttgc aaaaaacgat gatcacaaat cattccgcaa cagataaaag tcaaaaagtc
    18061 aatctgcttt ccgtccacct gccgctcgct gattgtttga tcataaacga tgcgttctgg
    18121 cagattgatg gtttgcagca ctacacgatt tggtagctgc aaaatcgtcc gctgcggtgt
    18181 taatgctcgg cttagagaac ggatcgatac agggatcatc catagtgatg aatcaagggt
    18241 tgtatgtttt tgaaaaaaac ctgccagatc catgatccct tcatttccaa atccgatgat
    18301 aaacatggat tgattttcgg gatagcgttt gacgaaatca agcaggttct ttaattctgt
    18361 taaatgattg cattgagtgt tggcacaaat ataccagtca ttgtttgctt gatttgcaaa
    18421 taacgggttg atcttatctg caaacaaatc ataatagcgc tgattcgtta aaaataagat
    18481 tggctgatca atggaaagtt ctcttagctg actgactagc gtttctccgt aaaacacttt
    18541 cgtcaacgca tcttctactt gataatcaag ttccatacgc atccctcctc ttcttatttt
    18601 tattttacca ttagtaagca aattctagaa aagataagag caaagctcac ctcataactt
    18661 gcaaataaat aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg
    18721 aatctctgtc tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc
    18781 cctagagcca caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc
    18841 tggtttgaat gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt
    18901 acgacctttg cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac
    18961 tttttcgccg ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt
    19021 tgcatctttt ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt
    19081 tgccatggat agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa
    19141 ctttctgagt ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc
    19201 acgatatcat atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta
    19261 gtcatagaaa cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg
    19321 taaaatgcct attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat
    19381 gcttttctga tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca
    19441 ttgacaaaaa cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc
    19501 aggcgttttc ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca
    19561 ttcaacacag ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca
    19621 ggcagacgtt ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca
    19681 agataccgga attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc
    19741 tttacaagat cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa
    19801 cccgctactc gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg
    19861 atatcagaac ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg
    19921 tatacttttc cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt
    19981 tcagaaacga agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt
    20041 acacgttctt ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca
    20101 atcaaactaa acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc
    20161 actgcttcat ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga
    20221 tcgcctagcc ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact
    20281 ttattgactg ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca
    20341 tctgcgtctg tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca
    20401 atcgcgattt ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt
    20461 ccgcctgtgt cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga
    20521 tcacgagtta ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta
    20581 aaaatcgtag acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata
    20641 ttatttcctc ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc
    20701 aagcatagat tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt
    20761 tcaaaagaaa aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa
    20821 ttatttttca gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa
    20881 aatgtctccc atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt
    20941 tggttcttct tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg
    21001 ttcttctgga tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc
    21061 atgtggtgtc gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc
    21121 agggaacact tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac
    21181 tgtgccaact ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat
    21241 tgacaaggaa acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc
    21301 attgacagcc aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg
    21361 gactagaccg tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac
    21421 acgtccactg agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc
    21481 tctttcgttt tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat
    21541 ttcgatgatt ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg
    21601 atcttccacc attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc
    21661 tttgacaaca tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat
    21721 atcttcccaa acttttttag catctaaacg gcgttttgaa agtagatagc tgccgttttc
    21781 tttatctttg ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac
    21841 ttcgctgata tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac
    21901 tccagcccct tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt
    21961 aacaacatcg ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt
    22021 gttctcttga ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc
    22081 gtaagtacgg tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt
    22141 cgttttcctt gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa
    22201 ccttttttca aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc
    22261 gtatcgatac gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca
    22321 taatggtctc tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt
    22381 tcttggttct ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt
    22441 ttcacttctg cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca
    22501 ccgcgttccc ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca
    22561 gagacttcag aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt
    22621 gatgctatga atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg
    22681 atcagcgaaa ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa
    22741 gttactgcgc gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat
    22801 atcttggcaa cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt
    22861 gtcatgatat aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta
    22921 attcttaatt cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca
    22981 aacaattgat cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct
    23041 tgaacagtcg ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg
    23101 ctgcttgaat cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct
    23161 gcaatactgc tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt
    23221 gtactagatt ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt
    23281 tctactactg atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca
    23341 tttctgatcc agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa
    23401 aatagcgaaa gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa
    23461 acttcatcat ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct
    23521 gtagattgat gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat
    23581 tataccatag gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata
    23641 aatttagtaa aatcgttccc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg
    23701 gcagaagttc tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt
    23761 tttcgccaaa ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa
    23821 gcatctgctg caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat
    23881 tttctggttt ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg
    23941 gagcttgctc tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa
    24001 gttgattaaa gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac
    24061 tttcttcctg atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg
    24121 gcaaatcata atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat
    24181 tcgtagcaat gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt
    24241 gagctgcatc aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat
    24301 acaatcgttc gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt
    24361 ccatgaagtg ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat
    24421 tttgccgatt gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta
    24481 tatctcttgc tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta
    24541 aaagctttcg gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag
    24601 acacgcaatg agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta
    24661 aaagagaagg attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat
    24721 gacggaggac gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt
    24781 tctcccctcg tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca
    24841 cgatgatgac cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg
    24901 ttcctgtagg caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt
    24961 cttggccggg acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc
    25021 tgtatcgctt cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat
    25081 tgtccatcta cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc
    25141 aaacgttcga ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag
    25201 gcccactcaa taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc
    25261 tggtcaattg tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa
    25321 ttttgctgta ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt
    25381 tgtgctaaca aaaggtggat acatctgctt tgttgcagat aaaccgctga ctcatcattg
    25441 ttttttgcca gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct
    25501 aaaaaatctg ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca
    25561 gtatcaatca aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa
    25621 gtataaaaag gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa
    25681 attacttgaa cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg
    25741 tcaaaacgca aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc
    25801 gttagctttg cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa
    25861 atttgataaa acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg
    25921 tgggtaaatc cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga
    25981 gaactaatcc gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta
    26041 ttctccttaa gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct
    26101 gtattgcatg cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag
    26161 gaatcgtttt atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag
    26221 aaacatatgt aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata
    26281 aaatataaat ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa
    26341 gccgggatct gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct
    26401 atctatagtt aattgactag ttttttcacg gaccctatag gacccattta tattatttca
    26461 actttttcag ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa
    26521 tttctgcctc tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc
    26581 cattgattga tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt
    26641 cctgattcaa tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg
    26701 tcaatgttcc ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt
    26761 ctgaatgatt cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga
    26821 tcacataatc ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca
    26881 ctgctagatc aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt
    26941 gcgcccaaat actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg
    27001 gagactttcc atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc
    27061 cttcttttac caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt
    27121 aataaccggc tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa
    27181 atattttcac tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg
    27241 attatacctt aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac
    27301 ggaatatcca tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg
    27361 atacacagct tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc
    27421 attcgcctgg tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg
    27481 gttcatcgta cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt
    27541 aatatcgcga tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa
    27601 tgaatcaagg aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa
    27661 tttagctact aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt
    27721 tgtttgttct ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc
    27781 atcttctgtt ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa
    27841 gaaattgatt gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc
    27901 attcactcct tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc
    27961 accttgttct tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt
    28021 ttctccagag taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga
    28081 aaactcgttc gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat
    28141 taaaggaaat tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata
    28201 tttttctaaa caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt
    28261 cttgaattcc acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg
    28321 acctttgtca tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa
    28381 tggttttgtt tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat
    28441 accgaataat tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt
    28501 aaatgttcta aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac
    28561 ttttttaccg aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg
    28621 gatcaattcg ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt
    28681 catgatatta tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct
    28741 cttcttctca aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca
    28801 aatgatgaaa gaaaaaacac tcactgaatg gagctattga cttatccacg tgcttatcga
    28861 tagccgttta tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt
    28921 tagaacattg ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat
    28981 ttaccggtaa atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt
    29041 acgatagcag atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg
    29101 attactaaat ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt
    29161 gcagcagcta agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat
    29221 gtaataatga tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag
    29281 atggtgtctg tgattaatcc attgttcata accacttgga taccacgagc aacagctact
    29341 acgattgcta caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac
    29401 tctttcatgc cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt
    29461 tctgggaagt accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc
    29521 ggaaggtttg ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt
    29581 gtccaaggaa tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca
    29641 tgtttttgtt ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc
    29701 atatcttctt tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat
    29761 gcataacgat acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga
    29821 agaccaatac cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa
    29881 ggatttaccg ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct
    29941 actactgtat caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc
    30001 gtttcttctg ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga
    30061 ataagcattt tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt
    30121 gcccctgttt tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt
    30181 gggattgcag caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg
    30241 attggcgcca tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat
    30301 ccagcgacga tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta
    30361 agtacagcga ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat
    30421 tttttctttt ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt
    30481 ttggctctaa tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt
    30541 gtaaaacttt tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt
    30601 aaagctttct tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct
    30661 tttaaaagga gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa
    30721 aaaaatactc aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc
    30781 caaaaaaaaa gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac
    30841 gttgttcgaa ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta
    30901 aaaagacagc actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta
    30961 catagtttgc taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt
    31021 aaagaggcgt aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc
    31081 ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat
    31141 aataaatcgg gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat
    31201 tatccggaga aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa
    31261 ctccggccgc tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga
    31321 tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga
    31381 tcgcagcaaa catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc
    31441 ctcctgttag gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc
    31501 tattttccag ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat
    31561 cttctttatc cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc
    31621 aaaataggtc gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac
    31681 cttatataat ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta
    31741 acacttataa aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt
    31801 gttcagttca cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc
    31861 acgttttagt ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt
    31921 acgtccttcg tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa
    31981 aatttggaat ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg
    32041 tgcaagcatc gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc
    32101 atgttttgga tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc
    32161 cgaagtatcg taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag
    32221 atccgtaaca acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt
    32281 gtacaataaa tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt
    32341 gtcatgtttc ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg
    32401 tcctgcaagg atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac
    32461 tttctgcaat ctctccatta caattattct cctttattca ttccatcaaa gaatagatct
    32521 ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat
    32581 gatttcaaac caaaataatc taagaagtag ccagttgttc catataatat cggccgacct
    32641 ggtccttcta ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct
    32701 cctgaaacct gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa
    32761 gcgatgattg ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca
    32821 tatcgcttaa gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt
    32881 tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact
    32941 aaacttgtga cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc
    33001 ccttcctcgc caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt
    33061 atctcctact gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat
    33121 ctgccctttt ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga
    33181 aggtgactca aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc
    33241 gataaatacc attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt
    33301 ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt
    33361 gacttgtcct cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc
    33421 ttttgtgtaa taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt
    33481 atatttacgg tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa
    33541 ttcttcatcg tctgtgacga attcttggac aggaagcaat gtcttgcttt tgattgccat
    33601 caatgtagca gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt
    33661 atggatataa ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat
    33721 ttccatttgc tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt
    33781 aatattgatc tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca
    33841 atgtaaggct ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat
    33901 tcatctaaca tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca
    33961 tggatgatca gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt
    34021 gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc
    34081 tccttcaaca aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca
    34141 attttacggt tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc
    34201 cttggaaaat gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc
    34261 tgagtcgtag agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca
    34321 ttttctagca gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa
    34381 atcccagcct ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct
    34441 ttcccatgat tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt
    34501 gcttcgtcta agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc
    34561 tctttatctc cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat
    34621 ttcaatccaa tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca
    34681 cgatctctta tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct
    34741 gtcagagtca atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc
    34801 ggatcatgat cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag
    34861 atcatccgag taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg
    34921 atgactaaaa agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc
    34981 aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca
    35041 atattcaagt agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct
    35101 ctctatcata tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa
    35161 aaagccgtct agacactcag acggactggt ttcttttttc tttttcatag cattcctgac
    35221 agattccatg gaacgttagc cgatgatctt ttactaaaaa atgataacgt tcttctacta
    35281 cttgctccac ttcaagtaaa agatcctctt ctacttcctc gatcgtgccg cagttcaagc
    35341 atagtaaatg atgatggaaa tgtttggcgc cttcttttct caaatcataa cgggctaatc
    35401 catcattaaa acttacttta tcgacaattt tcaattctgt caagatttca agggttcgat
    35461 aaacagtagc aagtccaatt tccggactct tttttttcac taaaaagaag atttcttctg
    35521 cagataaatg ctctttctca ttttccaaaa gcacaagcag agtagcttct ctttgcggtg
    35581 tcagcttaaa ccccgcttca tgtagctgct gttttgtttt tttcatcgcc gaaatagcat
    35641 tcatgaccat ttactcaacc ccaacaattt ataataatta caaataaaaa aagatataac
    35701 actatgttaa ttaaaattat tataagctag ttaagaaaaa catgcaaggg attctcaatt
    35761 agattttttg actagttttg tcacaccgtt ttcttgtgta atcaatcctt gtttcaaaag
    35821 attacctaat gcgcgcttaa atgctccttt gctgatacca aaagctttca tgatttcttc
    35881 tggatccgat ttatctgtaa agttgatttg ctgatctgca gaacgttcta aaatggttaa
    35941 aatcatttgt gcatcatctg atatagcttc atatgctctt ggcttcaatg aaagattcag
    36001 tacaccgtct ggtcggacac caatcactcg accttttatc tgctcgccta gacgaggttc
    36061 attatatcgt tcagaaggat ggataaaccc aagatagaaa tcttccgtta aaacgtacgt
    36121 acctgttaat ttcaagcggt atgctgttcc tatcacatct ttgtttttca tttcttctgt
    36181 tccatatttg cttaaagatt tgaatatttt ttcatctgct aaactgcccc aaatacggtc
    36241 ttttgcatca actctaaggg aaatcatcaa gcgatcccct tttttaggcc atagctctgt
    36301 cattgtcggc aattcatcaa gagatacgac aaagtcttta tctggaagac cgatatcgac
    36361 aaacgcacct aaatcttttc gtgtagcagt aacgattcca aaagcgtaat gaccgatacg
    36421 gcttttagga atttgtgtag tgatccgatt ttcatgtttt tgattttggt aaccgaatcc
    36481 ttcaactgct tcaccgattt tgtgttcacc ttctgatttg cttaagccaa aggtttgtcc
    36541 gtttttttgg acaaagtagt ttttttcatt ttcatcaata ataatacctg taaatatagt
    36601 tgctaataaa tcgttcattt ttttctccct ctatagtgaa aggttttctt aatacatttc
    36661 tttgctatca taacataatg ttatctactt gaatagctga gccttggttt ggaaaggtga
    36721 aaaaaggagt atgacaacag ttgtgtcata ctcccctttt tcgaataaac ggtgttaaac
    36781 ggcaggctca cagcctcttt catttgccta aaattaattg ctcccatttc ctccgaacca
    36841 tccagtttta cagttgatgg ttacgatgta aaatggcaaa gcactttctc caatgccttt
    36901 catcaatgca gattttgttg aagtggcata tagattataa tagccttccc cattttcgat
    36961 tctggctttt tctaatctgt aacctccttc aacgatatat ccacgttgga tacatgtagc
    37021 aatcactttg ctttggacac ctggtgccca cgtccatgct ggattagatg catcggcaat
    37081 atcacttgct ctggcagatt tgattggctg attcgctgag tttgaagagg aaggggtcga
    37141 ggatgccgct tcttgctgtg ctgcttgtgc gaccgcagct tgttgagcgg cttgagctgc
    37201 agctgctgct tgagcggctt gttttgcttg ttcttcagct gcttttttcg cttcttctgc
    37261 ttttttcttt tcctcttctt cttttttctc tttatcagtg agcatcttat ctacttgttt
    37321 caattgttca ttcaactgag cagatagatc tgtatttttc actttttcta ctgcttcttt
    37381 tgccgtcttg tattggtcgc gtgttgctga atccactact tttccgtctt taaagactat
    37441 agctgttttt tctttagctg tttgcaattc agcatattga tttttagctt ctgtttgtgc
    37501 ttgttgaatc aattttccaa aatctgtgtt atccgtttcg attgcttgga tctctttatc
    37561 tgctttcaac ttgatgtcat ctgctagatg atcatcgacg atggcaggtg ctatgaacca
    37621 ctcattcact ttttggatat ctcggatctt agattggata tcttggtaag ttgtttctaa
    37681 ctctgaatat cctttttcat tcttaagact ggataaatca ttttttagtt ttgataaatc
    37741 ttgatgaatc atatctgcac gaataaattg atggtgatca tctgtataaa aggcagcaag
    37801 tttctcatga agttcttcca tttgttgctg tctttttgct tctgctgcta cttcggcttc
    37861 tactttttga tgatggtcat aataagccca gcttcctcca ccgagtagaa gtactgctgc
    37921 taaaacgaga taaggtactt tttttgattt tttcttttgc gctttatctg gatctgtttt
    37981 ttctaagttt gattcttgat gtgttttttc agtatctggc acgattgtta ttgatttcgc
    38041 ttctgcttca aacagcttag gctggttctc ttctttttct atttttgctt caagatcagt
    38101 accctctttg cttacagatt gattttcagc atcggattca ctatttattt catcgggctc
    38161 tgttgacttt ttgtctgtgt tttcgagtat ttcttttttc tctgcttccg gcagtttatt
    38221 cgttttttcc ttttctaatt gtttttcagt aaatacttca gttttttctg tgatatccgt
    38281 ttttacgtct tttattttta ctgtttcttt acctgtggcg ttttctctat cagagtcttt
    38341 ttcagtttct tgttcctttt ctggctctac tttgttttcc tgtgcaattt tttcactgat
    38401 cattttgagc agctctttcg cgtgctcttc ttttgtatct tcacgatgtg ctttgatata
    38461 agcggatagt atcgaatttt cttcctcatt atcgacgatt tctttcagtt cagctactct
    38521 tcttttttgt tttgattcaa gcgtttcttc ttgattcttt cctgattctt ccgaagtctc
    38581 atgaacagtt gctttcttct gttcattttt ctctatatta gttacttgat cgtgagcagg
    38641 tggttcttcg tcttctggtt ctgctttttc gaataattcc attacagatt ctagaggtaa
    38701 atctttcaat tccgaccatt cgattggatc gtttaattct gtatcaggaa agactgcatc
    38761 ttcagttgat ttatcctgca tagctgcatc tgtatttgtt tcttcaattg attcttttat
    38821 ttgctgatct tctgcagtta tttgatgacc gcagtctgga cagatagtat tctgctccat
    38881 ttttttcgat ccgcaatttg gacatttttt catcacttag cgatcggctc ctttactagg
    38941 tttacgctac catttaatct atcatattct atacgtcacg ccaatagctt atttttagga
    39001 tttacttaaa aataatgaat gaatttcctt cctatagaaa aaaaagcttg agactgcgta
    39061 gtctcaagct ttttttcttt taaaataaac caaacagttt tactgatttc tcttctggtc
    39121 aatagtccac gcttattcta agaaatcttt taattgtttt gaacgagaag gatggcgcaa
    39181 ttttcttaat gctttagcct cgatctgacg aatacgttcc cgagtaacac caaatacttt
    39241 ccctacttct tccaatgttc tcgtccggcc atcatctaaa ccaaaacgta agcgaaggac
    39301 attctcttcc cgatcagtca atgtatctag tacatcttcc aattgttctt ttagtaactc
    39361 ataagctgcg tgttctgctg gacttgttgc ttcctggtct tctatgaaat cacctaagtg
    39421 agagtcatct tcttcaccga ttggcgtttc aagggaaaca ggttcttgcg cgattttcaa
    39481 gatttctcgg actttttctg ttggaagatc catttccgca ccgatttctt ctggcgtagg
    39541 ttctcttcca aggtcttgca gcaattgacg ttggatacgt atcaatttat taattgtttc
    39601 aaccatatga actggaatac ggatcgtacg tgcttggtca gcgattgctc gagtgatggc
    39661 ttgacgaatc caccatgtag cataggttga aaacttaaat cctttgcggt agtcaaattt
    39721 ttcaactgcc ttcatcagtc ccatgtttcc ttcttggatc aagtcaagga actgcatgcc
    39781 tcgtcctaca taacgtttag cgatacttac tactaaacga aggtttgctt ctgccagacg
    39841 ctgtttcgct tcttggtcgc cttcttcgat tttcaatgct aacgctactt cttcttccgc
    39901 agttaaaagg gagacacggc caatttcttt cagatacata cgaaccgggt cattgatttt
    39961 tacaccggtt ggcgcagaaa gatcttctgt ttttgcttgt tcagcttttt tctcagcact
    40021 cttcaagctg tgaattgatg gatcaccatt ttcatccacc acactgatac ctgcgtcttc
    40081 aatcttttgg atcaatttgt ccatttcatc tgcattcaaa gtgaatggtg tagcaagctg
    40141 attggataaa tcatcatata caacttgtcg tttgggtttg ttttctttga tgaaagctgc
    40201 tactgctgct tcatacgttt ttttatctgt tccatttgcc ataaaagaag gccccccttg
    40261 agtagctatt tggcttgttt taactgtttc gttaaattga tgatttcaat cgtcaattca
    40321 agctcaagct gtttgtttcc agttcgtctg gcttcttgct gcatttgttt ttttaatgtt
    40381 atttcatcag ctaagccaga tttgctgatc acatctagca agtcttggat ttctcgtggc
    40441 gaactttctt ctgccaccgg taaatattcg atttccacca ccaaccgcct taaattttca
    40501 tcttgcaaaa agtctaaaaa ctctgcaagg ttgaattcat aatgttgtga gacatatgca
    40561 tcgaataaaa aataaacttc tgcatatgca tcatgaataa actgaaattc tcgttctttt
    40621 aaaatattac ggacagaaac ttctttaaat gcacgatata atagaatctg ctctgccttt
    40681 tctacttgcg tcaaaggtct ctttttccgt gcagtttctt gtctcgtaac ctgagtgtgt
    40741 tgactttcct gaggttcttg acgattttgc tgatttgtcc ttcgaagatc gcgcagttgt
    40801 ttttgtatcg tctctctgcc gatctgaaac tctgttgaca gttgagatag atacatatct
    40861 tgttcgattg gtgaaacaac tcggctaagt tcaactagta attcttcgag gtattcgatc
    40921 ttgtcttttt cattatctaa atttttccct tgttttaaat aacgtgattt aaaagtaaag
    40981 actgtttcgc gcccatgagc cactaattct tggaaagatg cttctccata cttacggaga
    41041 tattcatcag gatcaagctt ttctggaacg actacgacag aaagtgctaa tcgactgttt
    41101 tgccgaagca attcaatccc tcgattggtt gcttcaaaac ctgcgttgtc tccgtcatag
    41161 caaaagacca attctttcgt cagcttttcc atttgttgga tctgttgagc tgttagactt
    41221 gtccccatag aagctaatcc agttttgata ccagcctgcc atgcagcaat cacatccata
    41281 aacccttcga acaacaaaat ttctccgttt ttccggatat cgcttcttgc tttatcaaga
    41341 ttaaataaaa cttgtctttt attgaacagt tctgtttctg gactattcaa gtatttgggc
    41401 tgatcttttt cctgcccttt ttcaggtgca agccagcgtc ctgaaaaacc gatcgtctgg
    41461 ccttgagaat tacgaagtgg aaacatcact ctttgataaa aacgatcaga catacggcca
    41521 tcatcatgcg tgacaaacaa tccagagcgt tctaacaatt catttgaaat tgattcattc
    41581 ttgaatactt gctgaagaaa agaacgctca ttaggtgcaa aacctatgtt gaattcttca
    41641 attaattcta aagacaaccc acgactttgc aaatattcta aagctggctg tccagctttt
    41701 gtatgcaata gcatgtgatg atagatttcc gctgcttttt catgagcagc aatcagtttc
    41761 cccgtagcag attcaggatt ttggacagct atagtacgct gacgccattg atcttctaac
    41821 ggtatctgtt cgaagtctgc cacttttacc actgcttctg gaaaacttag accttctagt
    41881 tcttgaagaa aagtaaacac gtttccgcct ttaccacaac caaagcaatg aaaaatctgc
    41941 ttgtcttctg caacagaaaa agacggtgta cgttcttcat gaaaaggaca cagccccaag
    42001 taatttttac cagacttttt caactggaca tattgtccaa tcacctcaac gatattcgtt
    42061 tgactgcgta ctgtttcgat aacctcttga ggaatccgtt gagccatttt ttcaccccct
    42121 gctctattga tatcttttgg gtttaacaga agatatttca ggagaataac cggtaaaaaa
    42181 tcgcactaac tgaaatcagt gcgattttaa aggacacaat tatacatgtt tgattgtatc
    42241 atatttcatt gctttttcaa gtataacaca tttctatctc attaagcatc aatttgctac
    42301 tcttttcagt aactgatttt gaatatcagc cttccagtac aatactcgaa gaacaatatt
    42361 tacgagcatg aatacaaaga agtatcctac gctctctccc aaccctaagt ttacttccaa
    42421 aattccatgg aacagcattg cagctacata tatccctacg atcgtcgtta ctgctaggaa
    42481 gatgcgcaat gggttgaagg gataacaagc ttttacaact gccatgcaac tgatcccaat
    42541 cagcaagtaa tacatcaaag tagtagtttc agcatttccc catgcgaatg tttttccaag
    42601 tagatacacc gcaatgatat tcactactac aagcaaggca tttggtaaag cattaataag
    42661 tgctgtaggc aaataacggt aagtgatttt tcttttatct tgttcaaaag ataaaaagaa
    42721 ggaaggatac ccttcaatcg ctaaatcaat cagcgtgatt tggataggga taaacggaaa
    42781 tccaattgct gtaacggcac atataatcga taaaataaat gaataaatcg ttttgataaa
    42841 aaagatgcct gagactttcg tcacattgtt gaccactcgg cgtccttcaa acaggacttc
    42901 tggcaatgta gtaaaatcag agtccaacaa taccagattc gcaatctgac gtgttgctcc
    42961 atcaccttct gccatagcaa tgctgcaatc tgcttctctt aacgccaaaa catcattcac
    43021 accgtcgcct gtcattgcaa ccgttctgcc gctttctttc aattcattta ccagcaattt
    43081 cttttgctgc ggactcactc gtccaaaaac cgtatagcga tgagcagctt ctcgcacttc
    43141 catttcttca gtaatttgag aaagatcgac atacgcatca taattcggta atcctgcacg
    43201 tctagcgata ttggagacag taaaaggatt gtctccagaa atcactttca gatccacacc
    43261 ttcctcatga agataagcta gtgtttcttg agcgtttttt cggattggat catcgatttc
    43321 aaacagagca accggctcaa caaatgctgg tttttcttcg ttcaattgct gatttggagc
    43381 gatccccaac ataagtacgc gatagccatt cgcttgggct gtttttagac tgtccggaag
    43441 ccgatcttct tccaccaaac gttcaggcgc tcctacgtac acgactccca gttctggaaa
    43501 ttccatcgcg ccccatttgc gatcagaaga aaatgcgact ttgtttgtca catcataccg
    43561 attagaaagc tcaaaataat ctctcatagc ccgcatagtg atgttgttat cactgctttc
    43621 tgccagatac gtccccataa tttctgggat ggctttctcg tacagcggat gtagtgcttc
    43681 tacccgctgg atactcattt tcccttcagt aatggtccct gttttatcca agcataatgt
    43741 atcgacatgg gccagcgttt caattgaata catgtcttga acaaggattc gtttttttgc
    43801 cagtttggtt acccctgtcg ttaatgcaat acttattagc aataccaaac cctttggaag
    43861 catgcctaac aaagctgctg cagatgcaat aacagaaatt ttgatagaag catcgcggat
    43921 gaacagtgct tccaaaaata gtatgatccc caaaggaata atcacgaaac tagtgaattt
    43981 ggatactttg cgaatcgaat ctacaagttc agaacgaata gctttgtgag tctttgcctc
    44041 gctagtcaag cggacagcat aattatctgc acctactcgg ataacttcag ccatgacaga
    44101 accgcttgtc aaaaagcttc cagaaagcaa ttcagctcca gtttctttga caatcaaatc
    44161 agattcgcct gtcaaaagag cttcatttgc ttctgcccgt ccagatataa cctgtaaatc
    44221 ggctggtatc tgttctcctg ctccgatttt taccaaatct cctaatacca gctctttagt
    44281 agaaatcgat tgttctttac cgtcccggat cactagtact tctgattggg aaacaatcga
    44341 taatttccgt acaagatttc ttgcatgtat ttcttgataa attccaataa cgatattgac
    44401 gacaataatt gctaaatacg ccatgttgga atacgcacct acaaataaca gacaaatccc
    44461 aataccaaag ttcagaaaat tgaataacgt caatatatta tcgctgaata tttgttttgt
    44521 actttttgat gcatcttcct ggtagtcatt ttgctggcca gaagccattt tctgaactac
    44581 ctcttgctgt gtaagtccat tcattttctt tttctccatt ttccgccaac tttcaaaaat
    44641 aatcacgtgt ttaagtctag cgtgttccaa gattaaaaac aacttgtttt tataaaattt
    44701 atagaaaaac acacgaatga ttttacaaaa aagatgccaa cctttttgtt tgaatcaccc
    44761 gtgtgctgtt tttattatca atctatcttt acttatattg ttcctccaaa cggctcatcc
    44821 accaacctag tgctgtaccg gctataaaaa gaacaggaca agctagtacc ccagcaactc
    44881 ccaatcccgc gtaatcggta ggaatgatca taatcgttga agcaaaaact acacctaaaa
    44941 tgaaatggaa tagcttgcta tatgcctttg agaaaatgta atccatgatt tttgacaatc
    45001 caagaacaca aagaatccca ccaattccga tcggcacaat cacttcaagt cgtacttctt
    45061 taaatccgtc agccatcgct ttatacaatc ccatatacac caaaaaatta gaagggctca
    45121 atcctgggac gatcataccc aaaccaatca atccgccggc aatcagccat gttccaaaat
    45181 tctgatcgac ttgcccgtca aataattgag cgccgtataa taaaaacaaa taagccagaa
    45241 caaaagtaac aaccatgatg atcacatcag aagtatctct tccttttttc cctgcttctt
    45301 tccataacgc aggaacagtt ccagcgatac atccgacaaa aaaccataat atcgttgttt
    45361 catacgcacc taataaaaaa ctgactaaaa aagaaagtag aaagatgcct gtcactccgc
    45421 ctagaccaac aggcagaaaa aacaaaacat tttctttgaa attttttgta aggtgggcta
    45481 aaaaagaaat cagccgctca tacatgccaa agacagctgc taatgcacca ccgctgactc
    45541 ctggcaagat aaatcctgaa ccgataaaca ttcccttcac aaaccgtaac atccagtctt
    45601 tggttgtgta ccccacttct tgattttcca ttttttgcct tttttccact atttgcgtac
    45661 tccttctttc atttaatttt attcatgatt gaacatttcc tgctatttta cttaaagact
    45721 ttttcatagg tctctattat tttttcattc attttgacaa tatcaggata aaacatgtta
    45781 cctccattat gatagagata gccgccattg taaagcaagg actggatccg ccaatatcgg
    45841 tattgtttcc cttcctcatt tcctaaaagt gggctcagca catctttaga atacgcttct
    45901 gctaattcta ctgtgttttc tccgccatga ttttttacat aggcaatata atctttccca
    45961 aaattatatg cttggacagc tgtccataaa tcgcatcctt gtttctttgc ttctactatc
    46021 atttccgcca aatatttgac accttgatca atactgtcag ctggattatc aatgatctca
    46081 gcttctccat gcagactttc cgagctctgc atgagatctg tcccttttcc ttttgattcg
    46141 gtcaatatta tcgccttgat caaattctcg tagttttcca tatgataatt ttttgcagct
    46201 gttgttattt ctatttgata gctttccact cgttttaccg aacgataagt tcttcctcat
    46261 aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt aaaaaagaat acggataata
    46321 gataatcgtt ttcgcttcat ttcatcacct agttattttg acatcttttt ttatcttact
    46381 atttctttat cttcttggct atttcttcct tgatttctgt gtacgcctta agaaaaaatg
    46441 aattttcctg tattttttga catcttacga aaatgatcgt tgtttttcag taaaaacaat
    46501 tgcccaatga ctgatataat cgttatactt tacacagtag gtgattatgt gaaaaaagaa
    46561 aagaaatatg caaaaatgcc agatgggagc gaaatttact atgaaaagag cggtcaaggc
    46621 ttccctctct ttttactgca tggcaatgac ggcagcggcc gctttttttc agaacaggtc
    46681 cctgtactcg aacgctacta taccgtgtat ctagtagata gccgaggaca tggtcgttcg
    46741 acaaacgaag catctatgtt gaattttcag ttaatggccg aggatctgaa tacgatcatg
    46801 ttgctggaaa aaatcgatca ggcagatttt cttggattta gtgatggagc aaatctagca
    46861 ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat tgattttaaa ttctggaaac
    46921 acgcttgtta aaggcgttcg tttttctgcc agagtcatca gtaatattca ttatgcatgg
    46981 gtatggttac tctctttatt tcgcccaagt cttcgcaaaa atcttttagt tatcaaactt
    47041 ttgctgcatg acatcgggct tacagaaaat gatttaaaaa aaatcaactc ccccactttg
    47101 atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt ctttgtatat agctaaaact
    47161 ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac atgaattagc tagaaaagat
    47221 cctgaacgct tcaatcgaga agttcttcaa tttctttctg aaacataact attaggaata
    47281 gaaggtgatt tatttgttaa aaaataccat acaatggctg aaaaaccacc taggactgtt
    47341 caaaacaatc tttttgattt ctgtcattgt cattattgtc ggtgaattga tgtccatcgg
    47401 taaaactcta tctatccagc aattaggaga aacgtttgcc actatacctg tatggaaaag
    47461 cggactaatg ctattgattg gtcttatctc tgtgttacct atggttggtt atgatattat
    47521 tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac ctgtttgaga caagctggct
    47581 gatcaatacg atcaacaata ttgccggttt cggcgggttt gttagtgtgg ggcttcgctc
    47641 tgaactatat ggacaaaaga aagagagcag aaacgtgatc caagcattat cgaaagtttt
    47701 cttgtttttg atggctggac tttctattta cagtctgctt tcttttttac ttgtagtctt
    47761 cacgccagtt gctccttttt tgaagcaata ttggatatgg ttgatcggcg gcggtctata
    47821 ttttcctgtc gtctttttat ttactacttt caaaaagaaa ggattccttg gtggtacctc
    47881 acttaaaact caaggacagc tattgctagt ttcattgttg gaatggaccg gtgtcctgtt
    47941 tagtttcctt tctgttggtt atttaatgga aatccggatt gatctgtggc aaacaattcc
    48001 attattcatt gctgcttcag tgataggaat cgtgtctatg attccaggag aaattgggag
    48061 tttcgatgta atgatgatta tcgggttgtc cgctattggt gtgccaagag aaatagtcgt
    48121 tgtgtggatc ttgctctatc gcctgttcta ctacatcatt ccgtttttga ttggaatcgt
    48181 ctttttcttc aaaaatatcg gttccacttt tgatcagcgt tattctggta tccctaaaca
    48241 attagcaaca gaaattgcgc ataaaatcgt ggttgtctta ttatactttt ctggaatcat
    48301 gctagtactt tctgccacta tcccgcaagc ttttacagag tttcgctggc ttcatagttt
    48361 gaacccattg aaatttcatt ttattatcca atttcctagt atactattgg gatttctttt
    48421 aatcattatg ggcagaggga tcgcagcacg tgtgaaacga gcatatttgc ctacaatctt
    48481 cttgattgtt ctagctttac tttatgtttt attaagtgat tttagtttca caccggtggt
    48541 tttcttgtct attctgcttc tgatcattct tgcttctaaa aacgaattat ttcgggaaca
    48601 attgatctat tcatgggagt ggcgtacgat cgatggaatc attattggtg cactgtcctt
    48661 gctttacatc gttattgggg tatacaatct gccggatttc cctcatcggc gtcaccattt
    48721 tatttccttt ttcttatttc cttctgaaaa gatttggttt tcgggattgt tggccatcat
    48781 cgctgttagt ttcatgatcg ttctatttgt tcatttcctt caaggagaaa agaaacagat
    48841 tggcgaagcc tttaatgaag aaaaggccct gaagatcctt acaacttatg gaggaaactc
    48901 cgatagtcaa ttgatcttct taaaagataa gcggatgttt gcctatgaaa aagacggtga
    48961 accaactgtt cttctccaat tcgcttgctt caataataaa tgtattgtaa tgggtgatcc
    49021 ttctggtaag aaagaagatt ttccagaagc aatcgaagct tttatcgaag aaacagatcg
    49081 actatgctat ttaccagttt tctacgaaac aagcgaagag attgttatga ttttgcatga
    49141 attcggctac gatttcatta agatggggga agaagcttat gtagacctga atagttttac
    49201 aacttcaggg aaaaaaatga agggcactcg tgctgtcttg aatcggatcg aacgagaagg
    49261 atttacattt gatgtcttac aaccaccatt ttctactgag caaatgagta ctttcaaaaa
    49321 tatctctgat aattggttgg gttcacgaaa ggaaaaaggg ttttctttag gctttttctc
    49381 tgaagattat ctacaaagag cgcctattgc agttgtcaaa aatacccaag aagaaattgt
    49441 cgcttttgca acgatcatgc ctacttacac gaacaataaa gtaggaacga ttgatctgat
    49501 gcgatatgat tctaaaaaag caccttctgg cagtatggat ttcttgttct tgcatttgtt
    49561 tgcctatatg aaagaagaac atatccaatg gttcaattta gggatggccc ctctttcaaa
    49621 tgtcggaact tccagaaaga gttttttaca ggaaaggatt gcatacttgg tttacgagtt
    49681 tggttctcat ttttattctt tccatggatt aagagaatat aaaagcaagt acgctacaaa
    49741 ctgggtctca cgttatactc tatattctag agatagttgg attgcatatg ttatgattgc
    49801 tttgctgatt attgataatg cgccagttga gcagacacaa ggtagaataa gtggtttgaa
    49861 aaaatggata cgaagaaagt attaaaaact gcataccgat agatgaacac tctcatgatg
    49921 aataaaagag actgaagtaa aacgaaagtt catgcttcta caatctcttc ttatacaatt
    49981 gaagaacaaa gagcggtcca aacagaaatc ctgtttggac cgctctttta ttttaaacat
    50041 ttttcattac caaaatattc gagtcgcgtt tcttatgctt cgatctctgt aacgatacct
    50101 gaacctactg tccgtccgcc ttcacgaatt gagaatgtcg ttccgttttc aacagcgatt
    50161 ggatggatca agtcaacatc aatcgtcacg ttgtcaccag gcatgaccat ttctgtatct
    50221 tctggcaatg tgattgttcc tgtaacatca gttgtgcgga agtagaactg tggacgatag
    50281 ttgttgaaga atggagtatg acgtccacct tcttcttttg tcaacacgta cacttctgct
    50341 ttgaattttg tatgtggagt aatggaacct ggtttagcaa gtacttgtcc acgttcgata
    50401 tcgtcacgtt ggatcccccg taacaacacg cctacgttat ccccagcttc cccgtaatct
    50461 aacgttttac ggaacatttc tacacctgtt acaactgctt tttgtgtttc aggtttgatc
    50521 cctacgatct ctacctcatc accgacacga acagcaccac gatcaatacg acctgaggca
    50581 acagttcctc gacctgtaat tgagaagacg tcttccactg gcaatagtaa tggtttatct
    50641 gtatcacgtt ctggtgttgg gatatattca tctactgtat ccatcaattc cataatagca
    50701 gcttcagcat ctggatcgcc ttgcaatgct tttaatgcgg aacctttgat cacaggagtg
    50761 tcatcgcctg ggaaaccata ttcgctcaat aactcgcgaa cttccatttc taccaaatcg
    50821 atcaattctt catcatcgac taaatcaact ttgttcaaga aaacaatcag gtattttaca
    50881 ccaacttggc gtgacaataa aatgtgttca cgtgtttgag gcatcggacc gtcagttgca
    50941 gaaacaacta agattgcacc atccatttgt gctgcccctg taatcatgtt tttcacatag
    51001 tccgcgtgtc ctggtgcatc aatatgcgcg tagtggcgtt tttctgtttc atattctacg
    51061 tgtgcggtat tgatcgtaat cccacgttca cgttcttctg gagctgcatc aatactagca
    51121 tagtcttgag gatttgctag gccttttttg cctaatactg tagtgatcgc agcagttaaa
    51181 gtagttttcc catggtcaac gtgtccaatc gtaccaatgt taacgtgtgg tttacttctg
    51241 tcataatgtt cttttgccat aatttacaaa cctcctaaat aatatagatc actgaaaaat
    51301 ttcagctctc acctttttat tatagtcttt ttttgactaa atcaaaaaga atacgtccga
    51361 cttacttttt gtaagattcg aactaagctt attatacgtc aatattggtc aaagtcaact
    51421 aagagcactc gtctgtctta tacgcgaaaa aagtaccttt tataaataaa aaaatgtgtt
    51481 ttttgagaaa aacttcaaga tagtccttaa ctttcttttg aagcaataag aacaaaatga
    51541 ggaaaagttt aaaattcagc tgttgccaac aactttcaat agacgaaatc ttctatttcg
    51601 ttaataataa ggaaagaggt gaagtacatg aaaaaattaa ttggcgtact tgtcgtttta
    51661 ggcatatttc tcggcggaag ttttgcagct tatcattatt tttacggcgg cgaagcttat
    51721 tatacaaaaa tcacgacgaa tggcgaaatg tcagctaccc aagcagataa cggagagaaa
    51781 ttcacgactt atacctacaa gcaagcggct tataataaag atggtgaaaa aaaacaggtg
    51841 acgcttcgcg aagaaagaga acatccatta aagatgaatg cgtatcttaa actcaaagtc
    51901 aatcctcgta aaggtgtatt aagctgggaa gaaataaagg cttctgaagt gcctaaaaat
    51961 gcggcagaaa aaatcgataa ttagtaaatg gtcttaaaat tttattagat aaaaagaggc
    52021 tgggacagaa gcgtttaact ccaagaaata agaaggagtt gcttctgctt ccgccgttta
    52081 tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtctcacgct ctttttttgt
    52141 tatattcgtc ttttccgatc cttctcttga cacaattcat ttaactgatc actggtactt
    52201 gaacacctaa tattctagtc atttttataa acgattcaat acttcatcca catttttgat
    52261 catcacggat atcgtaccat ccggattatt cacaaattca atcaaatctg ggttgcgata
    52321 tacatctact ggtacgatca gttcgatccc gtttgatagc ttgaattttt gttttccaaa
    52381 ttttttctca gaaatttctt gtacttcttt tacaggaggt gcttctggga caaatccttg
    52441 ctcttgtact tcttctttaa aagccatttt tgccgtgatg ttttccttga agactttttc
    52501 cgcaatcatc tcatgatcca accgaccttt ttcttctatc gtatcataga cagcttcttt
    52561 caaatcagcc atgatatcaa attcttctgt ctcaaatttt tttccaagat gcttggctac
    52621 tttttggatc acttttacat tttcttctaa agaaggtgca ggaacacttt cgatcactct
    52681 tccagaaaaa taaagctctt ttttgccaga aaactcatat tttttttcaa ttaattcgta
    52741 ggtccaatca gtcaaattca cagcgaatgc ttcatctgct ttttgtgttt tcgcccctag
    52801 aattgctcga ttcaaaatca agcggttgtc gatccctgct tcgtctgctt caacaaagtg
    52861 agtgtaagcc tcacgataat tgactttcaa taaagataag tacatcactg tatctaattc
    52921 atacaagaca acaaatacat ctgcactagg tgcttcttca ctttcttggt agatatcgaa
    52981 ccaccgctga acgattgctt cacttgcttc cacaaaattt tcttgagcga ttttaagtga
    53041 ttcaacgatc atgctttctt ctgctagttg tccagttttt gtttgagcag aagagatttt
    53101 ttggatcttc ttttgaagaa aatcacgaat atattctttt gtcagatcca gctctttttg
    53161 tgaaaagact ggagaaccag tttctctgtc aataatatgc agaattgctt tttttaaata
    53221 gatatccatt ttctcattcc cttctgtctt agtttacgga aaaaaaatca aaaataccag
    53281 aatcaaaatc attttctttt aaacaaaaat ttcaagagta gaaacaaaat ggattctttt
    53341 tttcgaaaaa tgcgctacac ttatagttga acgaataaaa aaggagctga aagtatgaat
    53401 tcattaacaa gcacttatcg tctatcaaat ggttacgaaa tcccggtagt aggattcggc
    53461 acatggcaaa cacctgatgg ggatgtcgct gtttcctctg ttaaagaagc gctggctgca
    53521 ggttatcgcc atatcgatac tgcccaagga tacaagaatg aagaaagcgt cggccaagcg
    53581 attaaggaaa gtggaattcc tagagaagag atattcttga caacaaaatt atggaatgca
    53641 aaccacagct atgaacttgt gatgtcttct tttgaagaat cattgaagaa gctccaaaca
    53701 gattatttag atctgttttt gatccactgg ccaaaccctg ttgcttttcg tgataattgg
    53761 gaacaagcaa atgcagatac atggcgagca tttgaagaac tgtatgaagc aggtaaaatc
    53821 aaagcaatcg gtgtgagcaa tttcttaccc caccatctag atacactagc aaaaacagca
    53881 aaaattatgc cgatggtcaa tcaagttttc cttgcaccag gcgaattaca gacaactgta
    53941 gttgagtatg caaaaaaaca tgatatgatc ctcgaagcat atagtccatt aggcactggt
    54001 aaaatcttcg atgtgcctga aatgaaacaa atcgctgaag cacatgacaa gacgattgca
    54061 caagttgctt tgcgttggtc gctgcaacac gaattccttc ctcttcctaa gtcagtgaca
    54121 ccaagcagaa tcaaagaaaa tactgaatta tttgattttg aattaacaga agaagaaatg
    54181 aaacaaatcg atcagttaga tggtgtagtc ggcaaagcaa aagacccaga tactacccaa
    54241 ttttaagtcg aaaggtgatt ttatggatcc aatcgaacgc ttgaattctc tctcggaaga
    54301 agttacccag acttttcatt ccgattttgt ctttttgatc gatgcagaga aaatccaaca
    54361 ttttcctgca agaaactgga cacatgatca aatcatcgaa gaactgaaaa aacggttcga
    54421 tcattcgcta atggtcaaac cctggcacga acatgaagtc atttattcac ctgaactacc
    54481 tgtttttgcc ttgataccga aaaaatagaa agaatggaac ggattatgta tatatacata
    54541 atccgttcca ttttttattt ttctttcaaa aaacgggaaa atacgcaaat ttttctgttg
    54601 taatttgcgt atacgcaagt ttttttgcat tactcctttt catttggctg tttttttctt
    54661 ataatcgaaa gggtatgagc gaaggagaga acatatgaag aaaaaacaga aacaaaaatt
    54721 attaaatcaa ttccgtcctt cattggatgg tgtaagagga caattattcc gttctctaga
    54781 agaagagagc ttgcgacgtt atggtttgcc gttacaagtc atgcttgatc ccaaaaaccg
    54841 tcaaatctta acgatcggat tagctgggaa aacaaatgaa gatacacgga tcaacgtgcc
    54901 aatcgatgac aacttcaata ctgtattaaa gagaattcgt tcaggagaaa acgggatttt
    54961 cgaacgattc cgtgacaatc ttcttattga aatcgtgtct tactggaatg atcaacgatt
    55021 gaaaaacgat gaaccaactg cgcaagctgt tgcgacacca gttacagaag aaacagtcag
    55081 tgtgccagaa gaaaaaacgg aaaaaccaaa agcggacaca actgaaaata cacgctcaac
    55141 tgatacgaat gccgctttaa gttttgctga tttttcaaaa gccgttgctg agtatcctaa
    55201 attttacaca gaacaaaatg aacatgaagt agttattaat gaaaaaaatg ctgatgaacc
    55261 tcgtaaatta gcaactattt ctatgactac tgaaaatgag ttcgttatcg aaaaagcact
    55321 cgaacggaaa tacaaagtca aattgacttt gattcctttg atcgaacaat ttgcagcgac
    55381 agcgattact gatcgataaa aattctattt gtcataaaag gctgggattt aaaatcccag
    55441 cctttttagt catataataa tttctacagc aagtaaaaag aaattccttc taacatcagg
    55501 ctcaaatatc agcttgacct gtccaacaaa tcacctatga tcctacaatg ttaggcgact
    55561 tctcttctac ctgaagatat cttggagttg cctgatttct taagatgcct aaccttaact
    55621 ataaaaaatt ctaataataa acatattttc tgatagagaa aaaa
//
LOCUS       DACCXW010000004        55268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCXW010000004 DACCXW010000000
VERSION     DACCXW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.004"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..847
                     /locus_tag="IVY71_000171"
     CDS             <1..847
                     /locus_tag="IVY71_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7984250.1"
                     /translation="KLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVETVAET
                     YQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVVG
                     ITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEKW
                     VLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENVT
                     TNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQG
                     NIVKE"
     gene            962..1882
                     /gene="pstC"
                     /locus_tag="IVY71_000172"
     CDS             962..1882
                     /gene="pstC"
                     /locus_tag="IVY71_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7984251.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1882..2766
                     /gene="pstA"
                     /locus_tag="IVY71_000173"
     CDS             1882..2766
                     /gene="pstA"
                     /locus_tag="IVY71_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7984252.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2781..3587
                     /gene="pstB"
                     /locus_tag="IVY71_000174"
     CDS             2781..3587
                     /gene="pstB"
                     /locus_tag="IVY71_000174"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7984253.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3603..4361
                     /locus_tag="IVY71_000175"
     CDS             3603..4361
                     /locus_tag="IVY71_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7984254.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4374..5051
                     /gene="phoU"
                     /locus_tag="IVY71_000176"
     CDS             4374..5051
                     /gene="phoU"
                     /locus_tag="IVY71_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7984255.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5289..5588
                     /locus_tag="IVY71_000177"
     CDS             5289..5588
                     /locus_tag="IVY71_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984256.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5614..6321
                     /locus_tag="IVY71_000178"
     CDS             5614..6321
                     /locus_tag="IVY71_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7984257.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6318..7085
                     /locus_tag="IVY71_000179"
     CDS             6318..7085
                     /locus_tag="IVY71_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7984258.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7185..8753
                     /gene="liaX"
                     /locus_tag="IVY71_000180"
     CDS             7185..8753
                     /gene="liaX"
                     /locus_tag="IVY71_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7984259.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9053..9370
                     /locus_tag="IVY71_000181"
     CDS             9053..9370
                     /locus_tag="IVY71_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7984260.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9370..9720
                     /locus_tag="IVY71_000182"
     CDS             9370..9720
                     /locus_tag="IVY71_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7984261.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9887..10825
                     /locus_tag="IVY71_000183"
     CDS             9887..10825
                     /locus_tag="IVY71_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7984262.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10840..11670
                     /locus_tag="IVY71_000184"
     CDS             10840..11670
                     /locus_tag="IVY71_000184"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7984263.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11714..12739
                     /locus_tag="IVY71_000185"
     CDS             11714..12739
                     /locus_tag="IVY71_000185"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7984264.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12754..13692
                     /gene="galU"
                     /locus_tag="IVY71_000186"
     CDS             12754..13692
                     /gene="galU"
                     /locus_tag="IVY71_000186"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7984265.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13817..14257
                     /locus_tag="IVY71_000187"
     CDS             13817..14257
                     /locus_tag="IVY71_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7984266.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14260..14862
                     /locus_tag="IVY71_000188"
     CDS             14260..14862
                     /locus_tag="IVY71_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7984267.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14930..16036)
                     /locus_tag="IVY71_000189"
     CDS             complement(14930..16036)
                     /locus_tag="IVY71_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7984268.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16246..17247
                     /gene="ccpA"
                     /locus_tag="IVY71_000190"
     CDS             16246..17247
                     /gene="ccpA"
                     /locus_tag="IVY71_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7984269.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17550..19895)
                     /locus_tag="IVY71_000191"
     CDS             complement(17550..19895)
                     /locus_tag="IVY71_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7984270.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20174..21076
                     /locus_tag="IVY71_000192"
     CDS             20174..21076
                     /locus_tag="IVY71_000192"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7984271.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21289..23064
                     /locus_tag="IVY71_000193"
     CDS             21289..23064
                     /locus_tag="IVY71_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7984272.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23321..24538
                     /locus_tag="IVY71_000194"
     CDS             23321..24538
                     /locus_tag="IVY71_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7984273.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(24985..26571)
                     /locus_tag="IVY71_000195"
     CDS             complement(24985..26571)
                     /locus_tag="IVY71_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7984274.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            26822..27046
                     /locus_tag="IVY71_000196"
     CDS             26822..27046
                     /locus_tag="IVY71_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7984275.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27188..28939
                     /locus_tag="IVY71_000197"
     CDS             27188..28939
                     /locus_tag="IVY71_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7984276.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            28939..30723
                     /locus_tag="IVY71_000198"
     CDS             28939..30723
                     /locus_tag="IVY71_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984277.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            31181..32032
                     /locus_tag="IVY71_000199"
     CDS             31181..32032
                     /locus_tag="IVY71_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7984278.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            32129..34015
                     /locus_tag="IVY71_000200"
     CDS             32129..34015
                     /locus_tag="IVY71_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7984279.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34056..34958)
                     /locus_tag="IVY71_000201"
     CDS             complement(34056..34958)
                     /locus_tag="IVY71_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7984280.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            35188..36591
                     /locus_tag="IVY71_000202"
     CDS             35188..36591
                     /locus_tag="IVY71_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7984281.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            36591..38024
                     /gene="ascB"
                     /locus_tag="IVY71_000203"
     CDS             36591..38024
                     /gene="ascB"
                     /locus_tag="IVY71_000203"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7984282.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38169..39095
                     /gene="trxB"
                     /locus_tag="IVY71_000204"
     CDS             38169..39095
                     /gene="trxB"
                     /locus_tag="IVY71_000204"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7984283.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39308..41035
                     /locus_tag="IVY71_000205"
     CDS             39308..41035
                     /locus_tag="IVY71_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7984284.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            41108..41401
                     /locus_tag="IVY71_000206"
     CDS             41108..41401
                     /locus_tag="IVY71_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7984285.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAIIQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(41485..42345)
                     /locus_tag="IVY71_000207"
     CDS             complement(41485..42345)
                     /locus_tag="IVY71_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7984286.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            42437..42865
                     /locus_tag="IVY71_000208"
     CDS             42437..42865
                     /locus_tag="IVY71_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7984287.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43027..43203
                     /locus_tag="IVY71_000209"
     CDS             43027..43203
                     /locus_tag="IVY71_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7984288.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43230..43676
                     /locus_tag="IVY71_000210"
     CDS             43230..43676
                     /locus_tag="IVY71_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7984289.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(43832..45211)
                     /gene="brnQ"
                     /locus_tag="IVY71_000211"
     CDS             complement(43832..45211)
                     /gene="brnQ"
                     /locus_tag="IVY71_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7984290.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            45917..46888
                     /locus_tag="IVY71_000212"
     CDS             45917..46888
                     /locus_tag="IVY71_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7984291.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            46912..49104
                     /locus_tag="IVY71_000213"
     CDS             46912..49104
                     /locus_tag="IVY71_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7984292.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            49121..49597
                     /gene="ybeY"
                     /locus_tag="IVY71_000214"
     CDS             49121..49597
                     /gene="ybeY"
                     /locus_tag="IVY71_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7984293.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            49575..49976
                     /locus_tag="IVY71_000215"
     CDS             49575..49976
                     /locus_tag="IVY71_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7984294.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            49991..50890
                     /locus_tag="IVY71_000216"
     CDS             49991..50890
                     /locus_tag="IVY71_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7984295.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51033..51845
                     /gene="recO"
                     /locus_tag="IVY71_000217"
     CDS             51033..51845
                     /gene="recO"
                     /locus_tag="IVY71_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7984296.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            52229..53146
                     /gene="glyQ"
                     /locus_tag="IVY71_000218"
     CDS             52229..53146
                     /gene="glyQ"
                     /locus_tag="IVY71_000218"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7984297.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            53148..55223
                     /locus_tag="IVY71_000219"
     CDS             53148..55223
                     /locus_tag="IVY71_000219"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7984298.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17900 a         9924 c        11766 g        15678 t
ORIGIN      
        1 taaattattc ttaccaataa taggattagc cattctttta tcaggctgtt caaagtggat
       61 tgatcgagga gaatccatta cggctgttgg ctcttctgct ttacagcctt tagtagaaac
      121 agttgctgaa acttaccaga cagaaaatcc tggaaaattc atcaatgtac aagggggcgg
      181 aagcggaact ggtttgtctc aagtacagtc aggagcagtc gacatcggaa actctgattt
      241 gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta gtcgatcatc gagtagcagt
      301 tgttgggatc actcctattg tgaacaagga tattggcgta aaagacatca gtatggaaaa
      361 cctgaaaaaa ctatttacag gcgagatcaa aaactggagt gaagtcggtg gaaaagatca
      421 gccagtagtt atattgaacc gcgcttcagg aagcggaacc cgtagcacgt ttgaaaaatg
      481 ggtattggac ggagaaacag ctgttcaggc acaagaacaa gattcaagcg gaatggtgcg
      541 ccagatcgtt gccgacacac ccggtgcaat cagctatgtt gctttttctt atgtttccga
      601 tgatgtagat acgttgaatg ttaacggtgt agaaccgaca gatgaaaatg tgacaacgaa
      661 caaatggacg atttgggcat atgagcatat gtacacaaga gatcatccaa aaaaactgac
      721 aaaagaattt ttagactata tcttatctga tgaagtacaa gataacatcg ttggagaact
      781 aggttatatc cctgtttctc atatgaaagt cgaacgtgat tggcaaggaa atatcgttaa
      841 agagtaatct ttacacaaaa tttacaaact tagctaaact tttacatgtt attaaaacca
      901 aattgatgtt caatttgtac actgagtgag tatgttaaac ctgattaagg gggagtcatc
      961 tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa cgttcaaaaa tggaacagcg
     1021 tggaaaattc atcagttttt tatgtatcgc gctgatcgtg ttggttgtat tatctatttt
     1081 ttattttgta gctagtaaag gattggccac cttttttact gataagatca atgtatttga
     1141 ttttctgttt ggtacagatt ggaatccaag ccaaatcgga gcaaatggga aaccaatggt
     1201 aggggcattg cctatgatca caggttcgtt tattgtgacc tttttatctg ctatcgttgc
     1261 cacgccattt gccattggtg cagcagtatt catgacggag atctctccta aaaacggaac
     1321 gaagatctta cagccggtca tcgagctgct tgtaggtatt ccttctgtcg tttatggttt
     1381 tatcggttta tcagttatcg tgccttttgt cagaagtatt tttggcggga cagggtttgg
     1441 tatcttagct ggtacatttg tgttgttcgt tatgatcttg ccgactgtca caacgatgac
     1501 tgttgatgca ttaaaggcag tgccacggca ctatcgggaa gcttcattag cgcttggtgc
     1561 cacaagatgg caaacaatct acaaagtcgt gctacgagca gccgtgcccg ggattttaac
     1621 agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc ttagctatcc aaatggtcat
     1681 cggtaatgca gcattgatgc caacaggact tactactcct gcttctacat tgacgtccat
     1741 cttaacaatg ggaatcggaa atacaatcat gggaacagtt gaaaataatg tgttatggtc
     1801 attagcgctg atcttgctat tgatgtcatt attctttaat atcgtgatcc gtatgattgg
     1861 gaagaaagga gagctgaaat aatgaacgca aaacgctcag ataaaattgc tacaggtgtt
     1921 ttatatatag tttcggggat catcgtattg attttagcag ctttactact ttatatctta
     1981 gttcggggaa taccgcatat ttcatggtcc ttcttgaccg aaccttccaa agcctaccaa
     2041 gaaggcggtg gaatcgggat ccagctattt aactcgattt atttgctttt gatcacgatg
     2101 atcatcagtt ttcctatttc tttaggtgcg ggtatctatc tttcagaata tgctggaaaa
     2161 aactggctaa cagatgtgat tcgcacgtcg attgaaatct tgagctcgtt gccttctgtc
     2221 gttgtcggtt tattcggttt cttggttttc gttattcaat tccagtatgg tttctcgatt
     2281 atttctggtg cattggcttt gactgtattc aacttgcctc ttttgactag aacagttgag
     2341 gagtctttac aagcggtcca ttacacacaa cgagaagcag gattggcctt aggtttgtca
     2401 cgctgggaaa cagtgataaa agtggtggtt ccagaagcga caccagggat tttgactggt
     2461 gtgatcctaa gttctggaag aatttttggt gaagcagctg cattgatcta tacggctgga
     2521 caaagtgcac ctgcgttaga ttttacgaac tggaatccat taagtgtttc aagcccgatc
     2581 agcattttcc gtcaagctga aacattagct gtccatattt ggaagatcaa tacagaaggc
     2641 acgatgcctg atggtgtttc tgtttcggct ggcgcttctg ccgtgttgat cattgctgtt
     2701 ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat tataccgaaa aatgacttca
     2761 gcctaaggag aagaagcaaa atgaaaaaat ataactggaa tgaaacaaat gtcatcacca
     2821 tgccaaaaga gaaagaagta gcactatata cggatgatct acatgtctgg tatggcgaca
     2881 atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa taagatcact gcactgattg
     2941 gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt gaatcgaatg aatgatggga
     3001 tcgctaatac gaaaatcact gggaaaatca tgtatcaagg cgtagatatt aatgcgcctc
     3061 aagtggatgt ttatgagatg cgcaaacgga ttggcatggt ttttcaacgt cctaatccgt
     3121 tcagcaagtc gatttatgac aatattacat ttgctttgaa acagcacggc gaaaaaaaca
     3181 aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca agctgccttg tgggaccaag
     3241 taaaagacag cttggacaaa agtgccttgg ctttatctgg cgggcaacag caacgtttat
     3301 gtattgctcg tgcgatcgcg atgaaacctg atatcttgct gctagacgag ccggcaagtg
     3361 ctttggaccc tatttcgaca ggaacggttg aagaaacact tgtaaacttg aaagaagact
     3421 atacgatcat catcgtgacg cataacatgc aacaagcagc tcgtatcagc gactatactg
     3481 cctttttcta tctaggaaaa gtaatggaat atgacaaaac taacaagata tttaccagac
     3541 caaaaattca ggcaaccgaa gattatgtgt ccggtcattt tggttaagac ggaggcctca
     3601 acatgacaaa agaaattatt tcatctaaag atcttcatct gtattatgga aagaaagaag
     3661 cactaaaagg gatcaatcta agtttcaatc aaggggaaat cactgcgatg atcggtcctt
     3721 ctggttgcgg gaaatccact tatctgcgtt ctttgaaccg gatgaatgac ctgattccga
     3781 atgtaacgat caccggaagt gttttatata aagacaaaga catctatggt ccaaaaacag
     3841 atgttgttga actaagaaaa gaaatcggca tggtattcca gcagccaaac ccatttcctt
     3901 tttctatcta tgaaaatgtc atctatggat tgaaattaaa aggggaaaaa gataaaaaag
     3961 tccttgatca agtagttgaa gaaagcctga aggcagcgtc tgtttgggat gatgtgaaag
     4021 acaaattgca taaaagtgct ttatctttat ccggtgggca gcagcagcgt gtatgtatcg
     4081 cacgtgtttt ggcagttaat ccggaaatca tcttattgga tgaaccaaca agtgcgcttg
     4141 atcctgtttc tactgggaaa atcgaaagta tgctgttaga attaagagag cggtatacga
     4201 tgatcatcgt gacccataat atgtctcaag cttcacgtat ttcagataag acggcattct
     4261 ttttagatgg tcacttgatc gaatacaacg atacgaagaa gatattcatg aatccagaaa
     4321 aacaagagac agaagattat atttcaggtc gatttggata aggaggaaac ggaatgctac
     4381 gtacacaatt tgaagaagaa ttgctgaatc tccataatca gttctacgaa atggggatga
     4441 tggtgagcag cgccgtgcac aagtcagttc gtgcatacat caaacacgat aaaatcttag
     4501 cccaagaagt tatcgaaaac gatataaata tcaataacat ggaaacacgc ttggagaaga
     4561 aaagttttga aatgatcgca ttgcaacagc cagttacaac tgatttgcgt atgatcatta
     4621 cggtcatgaa agctagctcg gatttagaac gtatggccga tcatgcggtg tctgttgcta
     4681 aatccacgat tcgcgtaaaa gggcagacga gaataccgga aatcgaaaaa gagatctccg
     4741 atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct agttgcttat gtcaaaacag
     4801 acgaaaaaga cgcacgtacg atcgccaata tggatcaaag agtcaatgac tatttcaatc
     4861 gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc tgagacagtc atcagtggaa
     4921 cagactactt gaatgttgcc agttatctag aacggatcgg agactatgtg acaaacatct
     4981 gtgaatggat cgtctacttg gctacaggga aaatcactga gttaaatacg aaccacaatg
     5041 agattttcta aactgaagct gtgattatgc cgtttgttta ggaaaagagg ttgtgaaatc
     5101 agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagtcaaaca aaaaatagct
     5161 cctcatattt ttcgtttggc tggacttatt accgcagatg caagcttttg aacaccgttt
     5221 attcgggaaa gggtttgtga caaaacatgt cacaaaccct tttcatcatt tcatagaaag
     5281 gataaagaat gcaaaaaaaa attagcatca tcactatatt ttttatttgt gtgcttactt
     5341 catgggttat tcagcatcaa gctgctttat atgaagtacc tgtcatcgag atcgaaaaag
     5401 tacgaataaa agaggatcat caattgatta caggaagact gatgaacacg gatcaaaaag
     5461 gagaatcgat ttatttatcg gttccttatc aaaaaaatca aatagaaaca gcaaatataa
     5521 aagcgagaca aatgtatcta gtccaaatgc atggagatga atggcagcta gtagcaaaaa
     5581 aaaaatgatg gctggctttt cttcttcaca agtatctcca ttggcgccat gttgcttatt
     5641 ggcggaaaac agggtctatt tgctttagga agtctcatca tcaatatcgt tttactttta
     5701 cttttgttgc tcatctttac taaaacagaa gggatttctc tattaaatat atctatcctg
     5761 ttcgtcgtta tagggatgat tattagcata ttggctttag atggatggaa tagaagcagt
     5821 ataatcaaaa tttcagctgc agttacgagt gtttttttag cattttttat ttgtcttttg
     5881 acaatgaatc attggaaaga ccaaggactg cgctatgagg aactgaatta tctgacacga
     5941 ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa cagctggagg aacactcgat
     6001 accgttatca cagtcattgc gacattagaa gaattgacta agcgacaacc agaaataaca
     6061 gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc
     6121 aatatccttt tgttcgtcta tatcagtggg tcagttccat cgcttctttt atatttagga
     6181 aatcaatggt cgttcaaaga aacgatagaa cagcatttat cattggaatt tttacgcgtc
     6241 attgctggca gcttaggaat agtattatcg atccctatct cggtcctctt ttttctgact
     6301 gtaaggaggc tgaaacgatg aatgtactta ttatacttgt tgggatttta gcaatcagcg
     6361 tgctacttat tggcggtacg caaggtatgt atattctttt aggcttattc atcaatttag
     6421 ggatcttttt cctgttgtta ttcggttatc atcagaagtg gccgatactg gcattgtcgg
     6481 tcataggatt tttattgatt gctgtggtta tcttattttt tatcaacggt tataatctaa
     6541 aaatgcgtgc agcttttgtg agtatactga tctttttatt ttgtttccta ctattgcttc
     6601 ctatcactga ttttttagcg atccaaggtt ttacatcggt agaactagaa gaattatctg
     6661 gcttagataa gactttagcg attgatttta gattacttac gagatcgttg cttctgatta
     6721 gtcttagtgg tgctgtttta gatgctagtg tagcaatttc tagtgggaca ttcgaagtat
     6781 atcaggcaaa tccgcaatta tctttttacc aattgcgcca ttctagtttc gctattgcta
     6841 aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc attcatggga agttctttag
     6901 cgttgatcct ctggtttatt gatttagaca ttccttttca gcaagtgatc aatgaaaaat
     6961 catttgttct agaatatacg atggctattc ttaccacatt gtctgcacta cttgttcttc
     7021 ctttaacctg cgtgactaca gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg
     7081 agtaaaagtt acaacttaag tcctatgaca tttttactga atcagtgcat aatatggatg
     7141 taaggaaaag cttgcaaaca attttgctta aggaggcaat ttccatgaaa gaaagagaac
     7201 gtattttaga tttagtgaaa aaaggaatcc tttcgactga agaaggctta gatttattag
     7261 aaagcatggc aactgaaaaa gacgaaaagc aaataaaaaa agaagcagac aaggtaactg
     7321 ctagtcataa agaaaaagat caggcaagtc aattgatcga taaacttgaa aacggcgaag
     7381 aagaaatttc tgagccagtt gatccaaagg aaagagaaag gcaagatcaa gaaaacttag
     7441 aaaagatctt agatgaacta gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag
     7501 taaatgctca attcgctgac aataaggcag cacgcaatga gaaacaggaa gcattgatgc
     7561 agctgaatac aaaagaagaa ttaggcgagt tgacagaaga agaattagca cagcgtcaga
     7621 cgttagaagc agaaatcaaa gaattagaag ctagtggcga tacactgtta gaagaacaat
     7681 tgaaattaga agcagaatta aaagatatcc gtaagaatca atggtctgag aaaaaagaaa
     7741 cgtttactga taagtttgaa ttgccagatg attggaaaga tcaagcaaca gatacactga
     7801 atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa
     7861 cattccaaac tgtttcagaa acagtgaatg acaacatgga atggaaagat gtcagcttac
     7921 gtgtccctgg gattgcgaca acgaaatttg agcatgaatt ttactatgaa gctcctgcag
     7981 caagtatcct tgatatcaag gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca
     8041 acgatgtgaa agttgaagct aagatcaaac tttacggaaa aatgggtgct gaaccatttg
     8101 aagcattctc tgaacggagc cagattgagg ttaatgaaga tcatatctcc ttccaaattc
     8161 caaataaacg tgtgcgtgca gaccttgttt tctacttacc taaacgagta tacgaccacg
     8221 cagctattaa acttctaaat ggcaacatca tgattgaaac attggaagct aaagatattt
     8281 atacaaaatc tacgaatggt aacatcatcg tggaccaatt aactgcaacg atgctggaag
     8341 tagaaggtgt aaacggcaat atcgatattc gaaacgggaa catcttagat tctattatcg
     8401 aaacagtaaa tggtacagtg acatttggtg cgacagcaga aaacctttct gtgtctttgg
     8461 taaatggtga tgtacgtctt acgattaaag aagataattt gaaaaaagta gaagcaagtt
     8521 cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat cggtttggaa ggacatgcta
     8581 aaacaagtct tggaagtatc aacagccgtc tgtccaatta cgaagtagtt cgtgagaaaa
     8641 aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc agatggtgaa atcacgcaag
     8701 tgcaactttc aacgacaaca ggaagtatct atttaaaaga tacagataaa taaagaggtt
     8761 gtgaaagaga tgttcagcct tgagcaataa gaagcgattt tgtaaaatag cttcccctat
     8821 tttacaaaat tgtgccttat tgccgatagg ctatctcttg aacaccgttt accaagagat
     8881 tatgaacctg ccaattatcg ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt
     8941 tttatgaata tggtaatttg tgaccgagaa ttttgcagaa agacaccgat taagaagaag
     9001 gttgtcacaa cgattttctt catttaaaat agtagaacag aggtgagaca tgatgaaaaa
     9061 aaagttgaca aaatccaata aaaatgttgt tttgacaggg actttagcag gaataggaga
     9121 atacatcggg attgatccga ctgttatccg tgtgctttat gtattcctga gcttagttgc
     9181 ttttggaagt ccaatcattt tatacatttt attagctttg attatcccat ctgcgcgttc
     9241 tgcacgaaca aactatggac ataacaatgc atattatgaa aaaaacaatt atcgtgaaca
     9301 aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa atcgatgatg acgaatggag
     9361 tgacttttaa tgtcgtactt tcaacgtatt ttagttaata cgttaacatt catatcattg
     9421 gctgttcttt ttcctgcgat ggtccatgtg agcagtattt ggacggccgt tcttgccagc
     9481 ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt tgacgatact gtccctgccc
     9541 tttacattag tcacattggg attgtttacg tttgttatca atgcagcgat tcttaaaatg
     9601 acctcctttt ttgtgggagc agaacatttt agtttttctt cattcggtgc agcgttgtta
     9661 gtagctatct tgatgtcgct tgtcaatgta attgtcagtg aacataacag tagaaaataa
     9721 aagaaagctg tgccgaacta tcggcgcagt ttttttattt agaatcagga atgcgttttc
     9781 tattacattg gtaattaaat aaaaacgttt tttttaattg gtagtacttt tggctagaca
     9841 atgataaaat gatagagaac actctaatag aaatgaggta gcatgaatgt cagaagttgt
     9901 aaaagttagc caattagtga aaaaattatc tttggaaatc gtgacaggcg acgagaaaag
     9961 cttagaccgt gtcatcgtaa ctggggacat ctcacgccca ggactagagt tgactggtta
    10021 ttttaattat tattcccata atcgtttaca gctatttggc agtaaagaaa tcagttttgc
    10081 ggaacggatg atcccagaag agcgtttgat catcatgaga agattatgta gtgaagatat
    10141 gcccgcattt attatttcaa gaggattacc ggctccagaa gaaatgatcc aggcagcaaa
    10201 tgaaaatggg atagctgtac tgcgttctcc catctcaact tctcgtttat taggagaatt
    10261 atccagttat ttagatggtc gccttgcccc tcgaacaagt gtccacggtg ttttagtgga
    10321 tgtttacgga ttaggtgtac tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc
    10381 gttggagttg atcaaacgcg gtcatcgatt gatcgcagac gatcgagtag atgtttatca
    10441 gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc ctggaacatc taattgaaat
    10501 ccgaggaatc gggatcattg atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc
    10561 tatgcaagtc cagttggctg tttatctgga agcatgggca aaagataaga aatacgaccg
    10621 tctgggctct gaagacacga ccgttgaaat cgcggaagtg ggcattccac aaataaaaat
    10681 ccctgtcaaa acaggacgaa acgttgcaat catcattgaa gtagccgcga tgaatttccg
    10741 tgcacgtaca atgggctttg acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat
    10801 tgaagaaaac tcaggcaacg actagcaagg agtgacaata tgttagcaca aatcaatcga
    10861 gttgcttttg agcttgtagg gattcctatc tactggtatg cattgatcat cgtatcaggt
    10921 atcattatcg ccatgtggct tagcagcaga gaggctgttc gggtaggatt aaaagcagat
    10981 gatgtgacag attttatgtt gtggggattg cctatctcca ttattggtgc tcgcttatat
    11041 tatgttttat ttgatcttcc ccagtatata gctgatccaa tccaaatatt caatatccgt
    11101 tcaggaggct tagctattta tggcggactg attgcaggcg cgatcacgct gtattttttt
    11161 actaaatatc attttatttc tttgtggact tttttagata tcgctgcacc aagtgtatta
    11221 ttagcacagg caatcggtcg ttggggaaat ttcatgaacc acgaagcata tggaccagac
    11281 acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat tcatcattga taatatgtat
    11341 atcgaaggga tctatcgtca accaacattt ctttatgaat cactttggag tttgattgga
    11401 tttattttgc ttttgttttt acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt
    11461 ttatgttatc taatgtggta tagtttcggg cgtttcttta ttgaaggatt acgtacagac
    11521 agtctttact ttttaggtgt gatccgtatt tctcaattat tatctgctgt gttatttgtc
    11581 ggtacattga ttctatttat ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc
    11641 tcattaggaa aaaatcaaca aattatctga tttttagagc gttaaaaatg aaaaaaattt
    11701 caggagtggt aaaatgaatt atagagtagc ggttttagga ccaggctctt ggggtactgc
    11761 tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg atttggggac atcgtgcttc
    11821 acaagtagaa gaaatcaaca cccaacacac gaatcaacgt tatttaccag atcattatct
    11881 acccgattcc atccaagcat tcaccaatat ggaagatgca gtaaaagacg cagatgcggt
    11941 gctattcgtc attccaacga aagcaatacg ttctgttgca aaagaactag tgcctaaatt
    12001 gaatacaaag ccagtgatca tccatgcaag taaaggatta gaacagggaa cacataaaag
    12061 gatctctgac atcttgttag aggaaatccc agcagaaaaa agaaaagctg ttgttgttct
    12121 ttctggacca agccatgcag aagaagtagc gaaacatgac atcactacga tcacagctgc
    12181 aagtacagat gcaaaagcag caaaatatgt acaagagctg ttcatgaata attattttcg
    12241 gatctatacc aatcctgatg tgatcggtgt agagatgggg gcagctttga aaaatattat
    12301 tgctattggt gctggcgcta ttcacggctt agggtttggc gataatgcaa aggcagcaat
    12361 catgacgcgc ggattagctg agatcagccg tttaggtgta gcaatgggtg ccaacccgct
    12421 gacctttatc ggattaagcg gagtaggcga tctagtcgtt acttgcacaa gtgttcattc
    12481 taggaactgg cgcgcaggca acttgttagg acaagggcag cccctagaag aggtgctagc
    12541 caatatggga atggtcgttg aaggtgtgaa tactactcaa gcagcgatgg aactagccca
    12601 aataatggaa atcgaaatgc ccatcactca aacaatctat gaagtgctgt atgaacaaaa
    12661 agaaatcaaa acagccgcaa aagaaatcat gttacgtgat gggaaaaaag aaaatgaatt
    12721 ctcattaaca attttttgaa ggagcgaatg tatatgaaag tcaaaaaagc agttattcca
    12781 gcggcaggat taggaacaag atttttacca gcaacaaaag ctatggcaaa ggaaatgttg
    12841 ccaattgttg ataaaccaac aatccagttc atcgttgaag aagcgttgaa atcaggaatc
    12901 gaagacatct tgatcgttac aggaaaagct aaacgaccaa tcgaagatca ctttgattca
    12961 aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga ccgaattatt gaaattagta
    13021 gaagaaacga cagatgtcaa cttgcatttc atccgtcaat ctcatccaaa gggtttagga
    13081 catgccgttc ttcaagcaag agcctttatt ggaaatgaac cgtttgtcgt tatgctaggc
    13141 gatgatatca tggaagatga agtaccattg acacagcaat taatcgatga ttacgaacgt
    13201 actcatgctt ctacgatcgc cgttatgcaa gttcctcatg atgaaacttc aaaatacgga
    13261 attatcaatc caggagaagt attggaagat gggttataca atgtcaaaaa ctttgttgaa
    13321 aaaccagatc caagcaaagc accaagtgat ttagctatta ttggacgtta cttattgact
    13381 ccagaaattt ttgatgtatt gtccgagcaa caaccaggag ccggaaatga aatccaacta
    13441 actgacgcaa tcgatacatt gaataaaaca caacgcgtat ttgcccgtaa attcacaggt
    13501 aaacgttacg atgtcggtga caaatttggt tttatgaaaa caagtattga atatggtcta
    13561 acacatccag aagtaggtgc acctttacgt gagtatatcc tgtctttagg taaaaaatta
    13621 gaagcagaag gaagcaagat cgatcataat ccaaaaggaa taacagaaat caaagaagaa
    13681 aagaaaaaat aacccttttt tctaacgagc ttcagctgct aattgaataa aaagtagttg
    13741 aagctcgttt ttatttgttt aaaaaacacg aaaatgctat ccttatagaa aagagagaca
    13801 agaagggagt ttgatcatgt caggaggaga aattgcagga ttgatcgcag caatcgcctt
    13861 tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa gtttctaaaa ctttagaaaa
    13921 agtagatcag actgtagcag aagcaaatac gacgatcgaa atcgtcacaa aagacgtaga
    13981 tcttttgtct agacaagtag aaggcttgct ggtcaaaagt aatgaactgc tcttggatat
    14041 caatggaaaa gtcgcaacga tcgatccatt gttcacagca gttgctgatt taagtgaaag
    14101 tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg aaagtcggcg gaatcggtaa
    14161 aacgacagct aaagcgacgg ttgccggaaa agtcggtggg acagcgatga aattttttaa
    14221 aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa tggctaaaaa aggtggattt
    14281 ttattaggtg cagtagtcgg aggaacagcc gcagcaatcg ccgcgttatt gttagcacca
    14341 aaatctggta aagaacttcg ggatgaatta gctgcccaag cggatgattt gatggatatg
    14401 gcttcagatt acacagatat ggcgaaagaa aaatcgactg agttaacagg tattgcaaaa
    14461 gaaaaagcgc aagatctttc tcaacaagca ggtgatctag caggtagtgt caaacaaaaa
    14521 gtaagtgaat cagcagatga agcaggaagt gaatctgacg atatcttgtt aaatcttaaa
    14581 gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg cagaagatat gaaaaaacct
    14641 gcagatgaat taggaagtat tgctaaagat acttcagaag atatcatgat tgatgtaaaa
    14701 gacgctgctg gtaaagcagg agatactgtc aatgaaggaa tgtcagaagc aaaaccagtt
    14761 gcagatgaca caaaagaaat gatgaaaatg gcaaaagaag atgcgaaaga agctaaaaaa
    14821 gaaataaaag atacaatgac agattcatct acaaatcact aaaatcacta aaatcacaaa
    14881 tttatagaat aaaaaggtgg gagcggacta gctctcacct tttttactct tatttttcta
    14941 aaactagtaa ttcttttggt gtagtcgtga atggcacaca gccatcgctt gtcacatata
    15001 cacagtcttc gatacgtacc cctacttttt caggaatata gattcctggt tcaagagaga
    15061 agcacatccc ttcttcgatt actaagtcat ttccttctac aagggaaggg aattcgtgga
    15121 cggttgttcc gatcccgtga cctaaacggt gattgaaata ttcgccgtag ccatagctgt
    15181 tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga cggacggctt
    15241 cttgtgcggc aagctgtgct tcaagagtga tgttgtatat tttttcttgg aagtcggttg
    15301 gtttttgata agcaattgta cgagtagcat cactgcaata gccattccaa acaacgccaa
    15361 gatcgaataa cacaagttca ttcggtgaaa tcttcgtgtt tcctggtgta ccatgagggc
    15421 ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa acaccttgtt
    15481 ttttcaattg gtattcgatt tctgcaatga tcgcttgttc agtcacgcct tctttcactg
    15541 ctttaaagcc aatctcaaat gcaacatcgg cccatttacc agcttcgatc agtttgtctt
    15601 tttcctctag agttttgatc agttgtagac gttggatgat ggaagctaga tcagtgtaag
    15661 aatccgttcc cgtcaagata tcagagatag catcgaagcg ctcgacagtc aaggcgttct
    15721 tttcaatccc tacattgcca ggctgaccat agcgagcacg aagttctttt ccgatgatct
    15781 cccaagggtt ttcgctgtct aaatagccac ggacatcata cgcccaacta ctgttttgag
    15841 catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa
    15901 gagctagtac acgctcatga ggatcacttt cgtatccgga aaagtaagcg atgtggccag
    15961 ggtcgctgat ataagccaat tcgacttggt gttgtgccat ccattgacgt agttcattga
    16021 tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc aataattgta
    16081 ccactgattt attaaaagac caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa
    16141 aacagagata aaacggttgc aattcttgag attatttgat attctatttt agaaatgtaa
    16201 gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga aaagcaaaca
    16261 atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc
    16321 gtaaatggca atccaaacgt caaaccagca acaagaaaaa aagtcttgga agttatcgat
    16381 cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca
    16441 gttggggtaa tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc
    16501 gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga tggtaacgac
    16561 caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg cgtgatcttc
    16621 atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt
    16681 gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta
    16741 tcagcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa aatcgctttt
    16801 gtcagtggtg cgttgatcga tcctatcaat ggacaaaatc gtctgaaagg ctataaagaa
    16861 gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac
    16921 ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac agctgtctat
    16981 gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg tggcgtgaaa
    17041 gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt
    17101 cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc tatgcgccta
    17161 ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga
    17221 atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt tgtgagcctg
    17281 ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatttcatg tttgatgaat
    17341 attgtgactc gtgattcgga gttggcagga gaacaccgtt tattcgggaa aaggggttat
    17401 gacaaaattt tgtttaatcc tttttttttg tcttaaaaaa gagagaaagt ggttgaaatt
    17461 acatttatcg tatataaaac aatccgtgta tagatgctgc attatgttca ccaccatcaa
    17521 aaaaataagg gactgtgtat tctattaaat caatcatctt tctcatctga ttctttggtt
    17581 gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata gttttcatcg
    17641 gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc ccatagcgat
    17701 tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt tccaggtgtt
    17761 tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc gaatttctca
    17821 tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt ggcaagataa
    17881 ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg ccctgcccag
    17941 ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta ttcattggta
    18001 gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct tgagatggca
    18061 tctttgaatg gtgtagtgat ttttgcattg atcacactgc gcatcatgtc attcatgatc
    18121 gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata aattacattt
    18181 cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc gccgtttgct
    18241 aaagcctgaa atccattggt ctgttgaagc gtcgtgacat ctactgtacc taaaggagca
    18301 gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta gtgctcgtaa
    18361 gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata agccgggata
    18421 ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc ttcgttagta
    18481 gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc ataatctgat
    18541 acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac aagaactggt
    18601 ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg attctgactg
    18661 taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat caatacattt
    18721 ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt ttctacgcca
    18781 ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata tcctttattt
    18841 tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc agacatatca
    18901 gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt catcacagta
    18961 taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa gtcttttgta
    19021 aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc acgatacata
    19081 ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt tagcgcacct
    19141 gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat ataagcagct
    19201 tgcggcagcg tcacatcttt tgcatctaca ccaaagattc ctcttgcagc ttcctggact
    19261 ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa atacgtagca
    19321 atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat ctcgtttgct
    19381 ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa
    19441 gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg taaaacagct
    19501 ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat aattgcagtt
    19561 ttcaataaag gtgaaatttt atcagatgaa atagtcgtgc gcatcagatc tgtctggatt
    19621 tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat gtttcctagt
    19681 tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag
    19741 ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt aataaaaaca
    19801 gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc tttttttctt
    19861 ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac taatggtttt
    19921 atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt tttggcagga
    19981 atttcttttt taaaagagtt aaaacgtgag aaaatgatct tttttataaa agagttctta
    20041 agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa aaatcaatca
    20101 ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt gcctttcaag
    20161 gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga aaatgctgtt
    20221 aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct atacaggcgc
    20281 ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg gaaaagcgta
    20341 catggcagaa aacggtctaa caaatatcgt cgttcatgcc ccttatatca ttaatttggg
    20401 gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag aagagattgt
    20461 tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc atgtaggggc
    20521 aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt tgacaaaaga
    20581 acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac
    20641 atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat tatccgttac
    20701 ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact tttcaggtgt
    20761 aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc atatcaatga
    20821 ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt ttggaacaat
    20881 cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata tttcgaaaat
    20941 cttggaaact ccctatgtcg gcaaagataa aaagaaagct tacgcaccat atggaaaaga
    21001 aatcgccatg ttcaaagcac aaatattcga tccagaagtg tttcctgagt tattagcaga
    21061 agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag gcacattgcc
    21121 ttgtcctttt ttgtttaaga aacaaaggag tgctttttgc agtctttttc atcatgcatc
    21181 ttctcccccc tctttttaaa tttagacaaa cagggtatac tggtagaaaa gtaaaaaaac
    21241 gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat gctgaacact
    21301 ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct tggcatagcc
    21361 ctattattta caattattcg tttgtattta tatagtaaag caatctatgg aacagacatg
    21421 tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt ggacggtaaa
    21481 tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag cttattttta
    21541 gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt cagtattttt
    21601 gccagtatat tggttgcact cgcgtttaaa ccgatcgtga agaacaagta tcttctttta
    21661 agtttctatt tttatttttt gttttctccg attagtttta cacatgaata ttccactcga
    21721 acgtatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg tttactagct
    21781 atttattacc gtaaatatag ttccagcaaa aaaatgctgc cgtggttttt agggctagct
    21841 gtttcttttc ctttcttttg gttcattcgc gaagattcca tctggcttct tccttttact
    21901 gtagtcgcgt taggcatcag tttcgtccag atcttgctag gacaagactt ttctttcaaa
    21961 gaagcaaaga agttaaaaga acgaccattt ctcctttcga aacaagaatg gctgaaactt
    22021 tcattgtttt tactccctct agtcggtttc tccatcgtct atcagtggat cgaatataaa
    22081 aacgagacaa catatagtat tgccgccatc aatgaccgtt cgagtggcga atttggacgt
    22141 ttgatgaaac agctgatccg aatagatgac gaaacgaact taaataaaga aaacagtgag
    22201 atatgggtct cgcatgctgc attaaataaa gcattagacg catctcctac atttcatgct
    22261 gtttctactc gtattgacga attgtatacg actcatgcgt ggacccaagg tggaaaagta
    22321 aaagaattac caggagatat cattttttgg gcattgcgag atgtggtaag cgaatctggt
    22381 tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa tcaagaatta
    22441 gaagaagcct atcaaaaggg aactatcaaa aagaaaaaag aattctatct gatgggatca
    22501 ggggatggaa agacgatgaa tgatctgcca atcgtatttg cttttctgaa atcaggatat
    22561 gcctatgcat ggcgatacaa agatttccat caagggggga attatagttt cggtacgcaa
    22621 gaacaagtcg aacaggccca agaattattg aatcttccac taatggataa ttggatcgat
    22681 agcagtgatc cgagaacacc tgatttccag ttgacaaaaa cagcaaaagt cgcgaatgtc
    22741 atgataaaaa tttaccaagt tctggccatt cccgtgctga ttgcttcaat agcagggata
    22801 ttacttatga tactaggaac cgcatttgca aaagctgatc gtgcctatta tagttcctct
    22861 ttgattgtag tcaccggatt aatactgaca caattggttt ttcttttcgg cgtctcgtgg
    22921 ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac tggcgcaggc
    22981 gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa attgaatctg
    23041 aaaaaagctt tatcttccaa ataaaaacat actaagatta gaggagaaca tcattgacga
    23101 atgatgttct cctctatttt ctatttttag attatagtga agagtgaaag aaagttcgaa
    23161 caaagccttg aggactatga gtccctatca aaaaccgaac ttctttactg tcattttaga
    23221 atcaggttta cgtatgttgg ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa
    23281 gcagtaaagt tctttcaaaa cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt
    23341 agatgtttca atgggacaca gctacagtgt cctatacaaa gacgacacct gcttatttga
    23401 agacgagatc gaacacactc aaagaggatt tcatgcatta ctcgaagaga tccagcactt
    23461 gcctgaacgt ccattgattg tttttgaagc aacggggatt tattccagac cagtcgaaaa
    23521 gttctttcaa gatcatcact tttcttactg tttgttaaac ccgttagaag cgaaaaaaca
    23581 gatggaagaa aacaccttat gcagttggaa aacagataaa tcggatgccc atcggctcgc
    23641 acagactcat cttaaaaatc aacgtcagcc aaaagaagta caaagcaatt tttacttaga
    23701 gatgcgcgat ttagcccgtt tttatcaaga aattgagaag aaaatcactc gtctgcgcat
    23761 ggatttacac aactgccttc agttgacttt tccagagctt gaacagttct ttttaaacag
    23821 gctgacccct tacgcattga tattgatccg tctttttccc catccagatt ttgtattggc
    23881 atctactcgg acaaaaataa aaaataaact gatcaacgaa acacggaaaa aaatctccgc
    23941 gaatcgtgcc gagcaaaagg cagatcaaat catccactat gcacagtgtg cctatccagc
    24001 tgttgaaaaa gacagtatcc actgtcaaaa aacgatctat tacgctgaac ttctccaaga
    24061 tttactcgaa caaaaagaag cactggcgac acaaatgatc aaaaaagccg aaggctctcc
    24121 ttgctttttg ttgtaccaaa cgttccctgg gattggcgca ctcacagccg ctttattgct
    24181 cggtgaatta ggagatatca cacgatttaa aacccataaa caattgaatg cctttgtcgg
    24241 aatcgacatc cgacggtatc attcaggtaa gtatactggc caagaccgca tcaataagcg
    24301 tggtaatccc aaagcacgga aaattatttt cttcaccatt cgaaatatga ttcgccagca
    24361 acgcgcagct cctaatcata ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc
    24421 taagaaagat aaggttgcca cagttgcttg tatgaacaag cttctgaaat gtatgcacgc
    24481 tatggttagg gcacatacag agtacgacta tgcgtacgcg gtctcagtgg accattaact
    24541 aaaccactta cagtatacca cttctttgcc cagatcgaaa attttttacg aacggggctt
    24601 tatttggtgt gccttgattt tgtcgctttc gtttatgagc tatttttctt acacctttta
    24661 cttgactaat cgtaggaaag ggaactgtga caaaagtctt gtcgcagttc ctttttccga
    24721 ataaacggtg ctcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacataa
    24781 aaagctgttt ttacttggct gttcttatta cttgatgcaa ggcagctttt tcacaacctc
    24841 tgatacacgg tgttccagaa gtaacttctt cggaaataag ccgaaatttc acaaaaattt
    24901 gaaaagcaat tttcttaaat tccttcttat ttcttgaagc tgcccacttc tgacacaacc
    24961 tcctttgtgt aaaagaatca gtttctactc gatttcttct atcgtttcct gcaaacgctg
    25021 ttcatatttt ttgtttcctt tatataaatc accaatcgtc catgccaaca ggagaaggac
    25081 aagaacaatg attacataag cgatttgatc agccaatgct agttgagctg aggtggcttg
    25141 ctcgccgaag aaactttcga tttgacctgg taatcctcgt aaattcaagt aagtcagact
    25201 aatcacagag aaccagccaa gtagttttac aacaaatgaa tttttgaatc ggctccccat
    25261 ctccgcttgg ctattggtca tcatcagtaa aggaatcatc gagaatggca atgcaaaagc
    25321 cagaaaaact tgggattcat tcatcaagtt attcaaagcg gtatgttctt cgatcgttcc
    25381 ttttgtactt gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca atcgtgtgac
    25441 tagtcttctt agccaaattg ggatacgcat atggatgaag ccttccatga tgacttgacc
    25501 ggtcaacgta ccagtaatag tagagttctg gccagaagca agaagtgcta cagcaaataa
    25561 agtagacaag ataccagatt ttgctacagc aatcaagata ccattgctca tagctgatgg
    25621 atcagataaa gcttcataca gaccaaaaaa ggaaggatct tggacagaac cggttttgaa
    25681 tacagccaca cccatgatca atagcagtgc attgactaga aaagccattg acaactggat
    25741 attagaatcc caagtagaaa agcgtaccgc ttgtgctaca tcttcttcgt cttggtgatc
    25801 gatcttccga gtttgagaaa ctgctgaatg aagatataag ttatgcggca tgactgttgc
    25861 accaataatc cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga
    25921 gaatgtgtga ctgttaggga taaatccggc aaacaaactg tgccaatcag ggttagacaa
    25981 gccaacttga tagacgaaaa cgaataaaat gacgaaaatc aagcagacaa ccaaagcttc
    26041 gattttacga aacccgactt ttgtcagcaa taaaaggacg aaaacatcta acacagtgat
    26101 aaatacggca attaccaatg gaatatcaaa aagcaggtag agggcgattg ctgcaccaat
    26161 gacttctgcg atgtctgttg ccataatggc taattcagtc aagatccata aaatgacacc
    26221 caatgtctta cttgtccgtg ctcggattgc ttgggccaaa tccatttggg tcacgattcc
    26281 taattttgct gccatatatt gtaaaagcat agcaatcaga ctagacatca aaataactga
    26341 catcaataaa tattggaaat tttgtccacc agtgatcgaa gtggaccagt tacctggatc
    26401 catataacca actgctacta atgcacctgg tccagagtaa gcaagaagtg ttttaaaaaa
    26461 actttttccc ttgggtacat ccactgttcc atttacttca gctaaagata aaccatttgt
    26521 atgttcaata agtcgatgct tatgaaattg tttgttggat tgattattca agaaaaaacc
    26581 acctttccta tttatctacg attacattct acacctcaga aagaaaaaat taaagaaaaa
    26641 gatttggtat gcctaatttt ttttcgtggc gtgtcttgct ggttaatgaa agaaattttg
    26701 ttcttctcta aaaaaaccac tattttttgc atgaaactaa agaatacgcc tttctttttt
    26761 caataaactt tagtaaaata aatcattgag agcaattcgg aaatagaaag gagagattta
    26821 tatggtatca acaggtattg tcgtacttat tgctattatt gctttgctaa tcggcgcagt
    26881 aggtggcttt ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa atcctcccgt
    26941 taatgaggac atgttgcgat caatgatgat gtcaatgggg caaaaaccat ctgagaagaa
    27001 aattcgccag atgatgcagc aaatgaagaa ccaagggaaa aaataggttc tgacccaaag
    27061 actttttgcg ggctgatttt ttcagtccgt tttttatttc gtaatttgtt agactattca
    27121 gactattcag caaaaaagac aagcacagac aatcctatgc tcttgataaa gacaaaggag
    27181 gagtaaaatg tcgattttta gaaaattagg ttggtttttt cgacaggaaa agaaacatta
    27241 tttaattggg attttctccc tattcgcagt ggccttggta cagttagctc ctccaaaagt
    27301 gattgggatc atcattgatg agatcgccga taaaaattta tcaataaaga tcatttcctg
    27361 gtggatctta ttattgatcc ttgcagctct cttacaatat ctttttcgtt atatctggcg
    27421 gacgaatatt tggggaagtg cagctcggtt ggagaaagaa cttcggcagc agctattcaa
    27481 acattttaca gaaatggaca atctctttta ccaaaaatat cggactggag atttgatggc
    27541 tcatgcaacg aatgatttga atgcaatcca aaatgtagca ggagctggta tcttgacctt
    27601 tgcagattca gtgatcacgg gcggaacaac gatcattgcg atgattttgt ttattgattg
    27661 gcgcttaaca ctgattgcgc tgattcctct gcctttacta gccgttactt cacgagtgct
    27721 ggggtcaaaa ctccacgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga
    27781 taagacacaa gaaagtatta ctgggatcaa agtcattaaa acatttggac aagaaaaaga
    27841 agatctcgcg gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa
    27901 ttttttagac tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc
    27961 aacaattatt ttaggtggaa agtatgtctt ggaaggcacg atctcaatcg ggcagatggt
    28021 ctcatttatt agctatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt
    28081 caacgtattg gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa
    28141 gacgcatatc gtggaacaac tgggtgcagt gcagactccg gcaaaaggcg agctgaatgt
    28201 agctatttcc tcttttaact atccgaaaga agaatcacca gcattagaac aaatcaaatt
    28261 tgcgatccaa gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacgac
    28321 aatcatcaaa ttactgatgc gtgaatacga tgattatcaa ggaaaaattc aatttggcgg
    28381 atgttcgatc aaagaatatt cattgaatgc tttattagga tcaatcggct atgtgcctca
    28441 agatcacttc ttgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact
    28501 ttcagaagaa gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga
    28561 attaccaaaa ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca
    28621 gaaacaaagg atttcgattg ctcgagcatt gatcaaaaat cctgaattgc tgatcttaga
    28681 tgatgcactt tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga
    28741 aataagaaaa gagaaaacaa ccatcattgc agctcaccgt ttaagcagcg tgatgcatgc
    28801 aaaagaaatc attgttgtcg atcaagggaa aatcattgaa cgaggaaccc atcatgagct
    28861 gcttgcatta ggcggctggt atgaacgaat gtgggagaaa cagaaattag aagcgaagat
    28921 agaaggaggc gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca
    28981 cttaaagaac agaagaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaga
    29041 acgttcatcg ttgctatctt atttgcgttt gccctgtctg tgatcaatgt cctattgcca
    29101 aggatcatcc aaacttttat ggatgatcat ctggctaaac agtcggctac gacacaagtt
    29161 attttgtttt ttgctggtat gtacttgttt ggagtcatag tcaaaagtat catttggttt
    29221 ttccaatggt atttgtattc catggcttca ttgaagacct atcagtatgt tcgggtaaga
    29281 ttatttgaaa agctgcatac actaggtatg cgttattttg atcagacacc ggcaggatcg
    29341 actgtttctc gagtgacaaa tgacacagaa acgctatttg aattttggta tgtctttttg
    29401 atggtattga ctgggatctt tgcagtcgtt tcttccttca ttgccatgtt tcaaatcaat
    29461 ggagaaattg ccttgtggac tttggtgttc ttgccgattt taggaatcgt catctggtat
    29521 taccagaaat tcagttctcg tatttaccgg aatatgcggg aaaaattaag tcagctgaac
    29581 acgaaattga atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct
    29641 cgtttagcta aagaatttga acaagtgaac aacgagtatt tagacacgcg ctatgcgatg
    29701 atcaaaacaa attcgattct gttaagtccg atcatcaatt ttctatatgc attggcgatt
    29761 gcgttggcgt tgactttgtt tgggatcgat gcgctgcatt ctcccgtaga agttggactg
    29821 atctatgcat ttacgaccta tgtccaggga ttttttaatc caatgtctca aatgatggac
    29881 tttttgagca cctttacaga tggaatggtt gccggtagtc gtattttgaa aatcatggat
    29941 acagaagaat atgcaccgca acaaaatcct ggagcagatc aagcgattac ttcaggtaag
    30001 atcgagtttc gcaatgtaag cttttcttat gatggagagc acaatgtatt gaacaatatc
    30061 tcttttgtcg ctaatccggg agagaccgtt gcattagtgg ggcacaccgg aagcggaaaa
    30121 agctcgatca tcaacgtatt gatgcggttt tacgaatttt atgaaggcca gatcttgatt
    30181 gatgatcatg acattcgaga atatccaatg gaagagttac gtaagaaaat gggattggtc
    30241 ttgcaagatg ccttcttgtt ttatggagat atcgcaggaa atatccggat gatgaatcca
    30301 actatcacag acgaacagat tcgccatgca gctgagtttg ttcaagcaaa caagttcatc
    30361 gatgaattac caaaaggata tcactcaaaa gtgatcgaac acggtgccag ctattctagc
    30421 ggacaacgac agctgatcac ttttgcgcga acaatcgtga cagatcctaa aatactagta
    30481 ttggatgaag cgacagcaaa tatcgatact gaaacagaag gattgatcca agaaggattg
    30541 acaaaaatgc gacaaggacg gacaacgatt gcgattgccc atcggctgtc cacgattcgg
    30601 gatgcagaat tgattttagt tttagacaaa ggtcatatag tagaacgtgg gaatcatgaa
    30661 acacttctag cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag cagtggtgtg
    30721 taaaaagtgg ttgtgacagg gctccgttca gctttaggtg ataagaaaaa aattgtaaaa
    30781 aaggctttcc agatttttgc ataaatttgg cttatcaccg ataagctggt actggaacac
    30841 cgtatattag aagttgggac agtagcatag tagcattttg acctaagcgc tagagtgaaa
    30901 ataaaacaga gcttcccaga ttcttcatct gtgagcgtta gtgtcgtaat taccagattt
    30961 aaaaaaaatc acgacagcta gtcagaagtg cccaatagcc gctaagaaag cgttgacaaa
    31021 agtgcttgct ttcgataata taaagaaaac gatacaactt ggatagtgat tgcctgatgc
    31081 aagctaaacc tgatgagtct cccatggttc catggacggc tttttgggtt tttctttttg
    31141 ttttggatac agccctatat atcatggaaa ggagtaaaac atgctgattg agaaagtact
    31201 gaataacaac gtcgtaataa ccagggatga aagaaatcag gaaatgatcg tgatgggaaa
    31261 aggtttggca ttcaaaaaga aagtgggtga cccgattttt gactcatgca tcgacaaaaa
    31321 atttcagttg acaaatgacg gactttcaca acagttccaa gaattggcac aagcgattcc
    31381 tcttgagtac ttagaaattt cttgtgaaat cattgaatat acaaaagagg ctctacaagt
    31441 ccagttgaat gattcggtgt atattgcatt gacggatcat ttgtatacgg ctatcgaacg
    31501 tcacaaaatg ggaatatttg taccaaatgt attgctgtat gatatcaaac aactatttcc
    31561 taaagagtat gccatcggga aaaaaacagt gaagaaaatc tcggataaat accacatcaa
    31621 gttatcagaa gatgaagccg gatttatcgc attgcattta gtcaatgcac aaggcattca
    31681 aagccaacaa gacaatacct acctgtttac tacaacaata caggatattc taaatatcgt
    31741 ccgctattac tataagatta catttgatga agattcagtc catttttttc gattcgtcac
    31801 acacctacgt ttttttctga cacgcgtcct agatggagaa aaatcagaag tgggagaagt
    31861 ttctgaggaa ttgtaccagg ttattcaaaa aaaatataag gatacgtttg gttgcgttga
    31921 gaaaatagca gactttttag ccgagatatt caactaccag atgtccacgg acgaaaaaat
    31981 gtatttaacg atccatattg cgcgtttagt gcatgagaat cgttgtaaat aaataaaaaa
    32041 aggaggataa attaacaaga acgcatgaaa taagggtagt gacattcagt tggctaaacc
    32101 ttccactaat aaattggagg gaatagaaat gggaaaatat caagaattag cgagcaaaat
    32161 tgtagaaaat gtcggcggga aggaaaatat caatggtttg acacattgta tcacaaggct
    32221 tcgtttcaaa ttaaaaaacg aagaaaaagc aaatgatgag atcttgaaaa atatggacgg
    32281 tgttgtgacg gtaatgcgag cgggaggaca gtaccaagtt gtcatcggga atcatgtgcc
    32341 agtcgtgttt gaagaggtta taaaagcagg aaaccttact tttgatgagg ctgtttcaac
    32401 agaaaaaatg agaccgtttg atatgcttat tgatggaata tcgggctgtt tccagccatt
    32461 ccttgctatt ctagctgctg gtgggatgat tcgtggactg acagcattac ttgtcttctt
    32521 aggcgcattt gaccgcggtt ctggcacatt tgttatgttt gataatatcg gtgattctgt
    32581 attccaattt atgcctgtta ttatcggttt aacagctgcc cgtaaattta aagtaaatga
    32641 attcgtaggg atgttgcttg gggcagcatt aatgaatcct agcttgtcgc tggaagcttt
    32701 gagtggtgca gcagaagcac cactgactac catattttct ggcactattt ttgaagcacc
    32761 tatttatcaa acagtattcg gcattccctg gattgcgcgt aactacgctt cttctgtcat
    32821 tcccattatc tttattgtgt tgttagctag ccaaattcag aaaccaatta aaaagttagt
    32881 acctgagatg atcgcgaatt tcttcgttcc attcttcacc gttcttatta caatgccctt
    32941 aggtttttta ctcgttggac cagcattcac attcgcaact gatattttaa tggctggatt
    33001 tgaaacactt ttagcacttt cgccagttat ttatggagca atcgtaggct tcttctggca
    33061 aattctagtg atgtttggtc tccattgggc aatcgtacca atgggattga tgcaattttc
    33121 tgtgaatggt tggcaaaata tcatgacacc tgttgcagta gtgtcttttg gacaaactgc
    33181 agcacttact gcactctact ttaagcttag aaacccaaaa gacaaagcaa tcgcaatacc
    33241 agccattgtt tctggtattg tggggattac agagccagct atttatgggt ttacattgcc
    33301 acgtaaaaaa atatttattt ttacctgtgt cggtggtgcg ttagggggcg cgtatagtgg
    33361 attaatgaat ttaacttctt ggaatccagg tggattaggc atctttacaa ttcccaacta
    33421 tattagacca gatggtgatt taacggatgt gattaatgta ttgattggaa tcgccattgc
    33481 gatggtggta tcctttgcgc tgactttttt cttttggaag gaggaagcag gtgaaacaga
    33541 tgatagacag aaaagatcag gtaaagaaat tgtaaaaaca ccgatacaag gacaaatcgc
    33601 tccactgaat gcagcaaagg atgcagcttt tgcacaagga acactaggtc gaggaatctt
    33661 gatctaccct gaaaaaggag aagtacgtgc cccttttgac ggaacagtca tgaccctgtt
    33721 cccgacgaaa catgcaatcg gaatggtttc tgaagcaggt ctggaattgc tgattcatgt
    33781 cggattggat actgttcagt tggaagggaa atattttgaa agcttggtgc agcaaggaga
    33841 aagagtgaaa caaggcgatc tgttgctccg cttcgacaaa aagaaaatcg aagaggaagg
    33901 atacagttta gaaacacctg tgatcgtcac caattacacg gattatttgg atattttgga
    33961 aaatcaaggg gagacggtcg ggccagacga tgcacttatc accgcactga cctgaacggt
    34021 taggtgaaag aatggactag gcttaattat tctttttagt ccacttcttt attttcaaaa
    34081 ttgaaaggct ctttcatgat ggctgagctg atagggcgtt tatcaaattt ttgtcctaca
    34141 gaaattagat ggactaaatt ctgttgataa ttttctgcaa aaacacaact atataacacg
    34201 tctagaagat aacaaatcga tgtattgatc gtaaaattac caattttcga atacagtcgt
    34261 tcgcgtgtcg taagtcgcaa agcggctgta ctcattgaag atagcgttga atcaccgata
    34321 ctggtcaaag ctaaaattgg aacatgattt tgtttcaata tttctgcggt acgaaggatc
    34381 acattgtttt cacccgtata agaaatcaga atcccgcaca tatcactcgt gcaattgtac
    34441 gcttcataga acgattcacc aaaagtggtg acaatcgtga catccatttt gatgcgcttc
    34501 attttcagca tgaaatcttg agaaatcaaa gtattcgagt tactggcgaa aatcacgatt
    34561 ttttttgctt gaagcattag ttgtttggct ttttgcagtt ggtcatgatg aatgagagaa
    34621 agtgtatctt gaatcgtcat ctgttctaaa gaagccattt tatttgcgat cgtcataata
    34681 ccgtctgtgg tggaaaaagg aagattcgca tctacgtctt gaaaattccc ttcaagatag
    34741 gctgtttcag ctaaaaaagc attacgaaaa ctgacccagc catcaaaacc catttttttt
    34801 gctacacgga tgagtgtgga cgggtggaca taacatcgct cagcaatctc ttggatggtc
    34861 atttcgccaa gtgctggaga atcgcttaag agaaactgaa tcacttcatt ttctgaagga
    34921 gaaaaggaga tttctttgat tttttcacgt aataacatgt agataccttc ttcttgtttt
    34981 tttacgtaaa ttataacttt tttgaaaatg atttgtaaac ggatttatgt tatatagctt
    35041 aataaagaca ttttttgaca agatatttac aaaaatccag tagaaaacac taacttttgt
    35101 aaatgttact ggaaagaaac gaatttctat tgtttgtttt gtttacaaaa aagataataa
    35161 aagtgtaaaa agtaaaggag tgagaacatg gcaaaaggaa tcaaaatcgc tacgattggt
    35221 ggaggcagca gttacacccc cgagttgatg gaaggattta tcaaaagata tgaggaatta
    35281 ccgatacgag aaatctggct cgtagatgta gaagaaggaa aagaaaagct tgagatcgtc
    35341 agtcagatgt ctcagcgtat gtgggatgct tctccgtatg aagtcaaaat ctatgcaact
    35401 ctggatcggg aagaagcatt gaaagatgcg gattttgtta cgactcagtt tcgtgtcggt
    35461 caattagatg cacgaatcaa agacgagcgg atccctagtt attatgggat gattgggcaa
    35521 gaaacaaatg gtgctggcgg gatattcaaa gcttttcgta ccgttccagt ggttttggca
    35581 atcgttgaag acatgaagcg tttatgtcca aatgcttggc tgatcaattt cgcgaaccca
    35641 agcgggatgg tgacagaagc agttatcaga tatggcaaat gggataaagt gataggatta
    35701 tgcaatgtgc cagtaatggc gatgatgaca gaaccaacaa tgcttggcaa gaaaaaagaa
    35761 gagctgatct acaagtttgc cggtctaaat cacttccatt ggcacaaagt agcagataat
    35821 aaaggaaatg accgcacggc agaattgatt gatcgtttat acgatgaaga caatggattg
    35881 ccgaaaaata tctttgatat cccgtttttc aaagaacaat taaaacaaat gaacatgatc
    35941 ccttgtggct accaccgcta ttactaccgt gaagaagaaa tgctggaaca tgctttagaa
    36001 gaatatcaaa cgattggtac aagagcgcag caagtgaaag aaacagaagc agagctgttt
    36061 gaattataca aagatccaga attggatcat aaacctgaac aattgcaaaa acgaggtggt
    36121 gcttattatt cagatgctgc ctgtgaaacg attgcttcga tctatggaaa taaagaaaca
    36181 cagatcgtgg tatctacaaa gaatgatgga gctgttcctg atctgccggc agattgtgta
    36241 gtagaagtca ctgcctatgt tggcgcacaa ggcgcacgaa atgtagcgtt tggttccctt
    36301 cctgctgctg aaagaggctg gttgcaagta atgaagaata tggaattgct gacgatcgaa
    36361 gcagctgtga ctggtgatta tggaacagct ttacaagcgt ttacgatcaa tccgcttata
    36421 cgatcaggaa agacagcaaa acggatcatg aatgagttgt ttattgcaca tcagcagtat
    36481 ttaccgcaat tcaaggaaac aatcagccgg ttaaaagcag aaggaatcga agtccaagat
    36541 gaactggcaa gagaattatc aagcaataca catgtaatgg aggaagtatg atgggattca
    36601 gaaaagattt tttatggggc ggagctacag cagccaatca atgcgagggc ggttatgatg
    36661 aaggcggacg tgggttagcg aacgtagatg ttgtgccaat cggagaagac cgttttcctg
    36721 tcattactgg aaaaaagaaa atgtttgaat ttgatgaaaa tcatttttat ccagcaaaaa
    36781 ttgcgatcga tatgtatcat cattataagg aagatattgc tttatttgca gaaatggggt
    36841 tcaagactta tcggatgtcc attgcttgga gtcgtatttt tccaatgggc gatgaaaaag
    36901 agccaaatga atctggtttg aaattttatg aggatgtctt taaagaatgt caaaagtacg
    36961 ggatcgagcc gcttgtgacg attacacatt ttgattgtcc gatgcatttg atcaaagaat
    37021 atggtgcgtg gcgttccaga gaactcgttg gtttttatga aaatctgtgc cgagtcatct
    37081 tcaatcgcta taaaggactt gtcaaatatt ggttgacatt caatgagatc aatatgatcc
    37141 ttcatgcacc attcatgggc gcagggctgt attttgaaga aggagaaaat gaagaacaaa
    37201 tcaaatatca agcggcacat catgagttgg ttgcaagtgc catcgcaacg aagattgctc
    37261 atgaagtaga tccggagaac aaagtaggct gtatgctggc agcaggagca tattatccat
    37321 acacttgcaa tcctgaagat gtttgggctt ctagaaaatc agatcgagaa aattatttct
    37381 tcattgatgt ccagtcacga ggagaatatc ctgcttatgc attgaaagaa atggaacgaa
    37441 atggcattca gatcaaaatg gaacctgggg atgccgcatt gctgaaagaa catactgttg
    37501 attttatctc cttttcttat tattcttctc gagtttcgac tactgacaac gaactcttgg
    37561 aacagactgc aggaaatatc tttgcttctg tcaaaaatcc atatctggaa gcaagtgaat
    37621 ggggttggca aattgatcca ttaggcttga gaataacgat gaatgatatt tatgatcgtt
    37681 atcaaaaacc actatttatt gtagaaaacg gcttaggtgc agtcgatacg cccgatgaga
    37741 atggttatgt ggaagatgac tatcgaatcg actatctggc agcacatatc caggcaatga
    37801 aagatgcagt agaattagat ggagtcgatc tattaggcta tacgacttgg ggatgtattg
    37861 atttagtatc agctggtaca ggtgagatga agaaaaggta cggattcatt tatgtcgatc
    37921 gtgacaacga aggaaatggc acattagccc gttcgaagaa gaaatcattc aactggtata
    37981 aaaaagtaat tgattcaaat ggagaaaatc tttcgaatga ttgatcaatg atttataaaa
    38041 gtctattgtt tttcagcgga tttttcgagt ataataaagc tattcgagaa acgcaagcag
    38101 aacaatagac tttttttcgc attggctgta aagtatgctt acttgattaa gtaaaggaga
    38161 atctacatat gtatgatgta atcgttattg gcgcaggtcc tgctgggatg actgctgctt
    38221 tatatgcttc acgttcaaac ttatccgtat tgatgatcga acaaggcgcc cctggcggcc
    38281 aaatgaacaa tacggcagaa atcgaaaact atcctggttt cgattcaata atgggtcctg
    38341 atctagctta taagatgtat gaaggtgttt caagatttgg gacagaaaat gcttatggaa
    38401 tcgtgcaaga tatcaaggat cacggcagcc acaaagaagt tatttgtgaa gataaaactt
    38461 acgaaggaaa aacagtcatt atcgcaacag gctgtatcca ccgtaaatta ggcgtacctg
    38521 gcgaggaaga atttgctgga cgtggcgttt cttactgtgc agtttgtgat ggtgcgtttt
    38581 tccgtaacaa acgtctagta gtgatcggag gaggagactc agcagtagaa gaagccatct
    38641 atttgacaca atttgcttct gaagtagtca tcgtccaccg tagagatgaa ttacgtgcac
    38701 aaaagatcat ccaagaccgt gcatttgcga atgaaaaaat ttcctttatg tgggatacag
    38761 tcgttgacga aatcgttggg aatgaaatgg tagtgacagg tgtgaaagca cgtaatgtca
    38821 aaaccggcaa tgagtttgat ttggcagcag acggagtatt catctatgtt ggactggatc
    38881 cattaactgc accatttaga gaagcagggt tgacgaacga agctggctgg attccaacag
    38941 acaacgaaat gaagacgaat ataccaggcg tttttgccat cggagatgtt cgtgaaaaag
    39001 atttgcgaca aatcactact gctgttggtg atggtgggat cgctggacaa caagtgtata
    39061 aatacttgga atctttagaa gaaacaactg cataagttta aaaagagtgg aagaacggtt
    39121 taattgttct tttactcttt tttttcgcgt gaaatctact gaatccgatt tatgcgaata
    39181 cttgcaaccg tgcttgataa aagaatcctt tcttgattta agcgtttttt ggtcctcata
    39241 ctttcatatc taataagtga atgctatact tattaagaac catatttcag aaagaggtgt
    39301 tattttcatg tcttgggaac aagtgtatga acaatgggta catgaagaaa atttagaaga
    39361 aaacttaaaa aaacaattaa atgatctggg tgaagatcct gaaaaacttg aagatgcttt
    39421 ttatgcaccg ttggaattcg gtactgcagg catgcgggga atcttagggc ctgggatcaa
    39481 tcgaatgaac atctatacgg taagacaagc aacagaagga cttgcacgtt tcatggatac
    39541 acaaggcgaa gaaacaaaac gccgaggtgt cgctatcgct tatgactcac gccatcaatc
    39601 accagaattt gccatggaag cagccaaaac attagcacat cacaatatcc catcttatgt
    39661 attcgaaagt ctacgtccaa caccagagct ttcttttgca gtccgtcatt tgaagacgtt
    39721 tgcgggaatc atggtaaccg catcccacaa tccagcagct tacaacggat ataaagtcta
    39781 cggagaagat gggggacaaa tgcctcctgc agacgcagat gcattgacga aatatgtccg
    39841 tgaagtcagc aatccgttaa aagttgaagt attatctgac gaagaagctg aacatagcgg
    39901 attgatcacg atcatcggag aagaagtgga tgccgcttat ctagaagaaa tcaaagcagt
    39961 aacgattgat catgaattgg tggaaacaat ggggaaagat ttgaaacttg tgtacacacc
    40021 acttcacgga acaggaaaaa tgttgggcga acgtgcgttg aaacaagctg gattcgaaca
    40081 attcgtacta gttccagaac aagctgtcgc agacccagat ttcactacag tcaaatcacc
    40141 aaatccagaa gaacactctg cttttgagta tgcgatacga cttggcgaga aagaaggcgc
    40201 tgatttgctg attgcaacag atcctgatgc agaccgttta ggtgcagccg ttcgtttgcc
    40261 aaatggctcc tatgaagtat tgaccggaaa ccaaatcgga gcactgtttg ctcaatatat
    40321 tttggaagca caccaatcgc gaggcacttt acctgaaaac gcagcggtat tgaaatcgat
    40381 cgtttctagc gaattaccta cagcaattgc ggaacactat ggggctacca tgttcaatgt
    40441 attgacagga ttcaaattca ttgctgaaaa aattcaacaa tttgaagaag atcattccca
    40501 tacttttatg ttcggattcg aagaaagtta cggttattta gttaaacctt tcgttcgcga
    40561 caaagacgcg atccaagcat tgctcttatt agcagaagta gcagcttact ataaaaaaca
    40621 aggcaaaacg ttgtatgatg gcttgcaaac gatttttgaa acctatggct actacgcaga
    40681 aaaaacgatt tctgtgacaa tgagcggaca agaaggttct gcgaaaatct ctgctttgat
    40741 ggaaaaattc cgtgaagaag caccaacaga atttgccgga gcaaaagtca tccaaacaga
    40801 agacttcaaa gagttgacga gaacagaccg tgaaggaaac gtagaaaaac taacgacacc
    40861 gccatctgac gtattgaagt atacgctgga agaaaatggc tggatcgcta tccgtccttc
    40921 aggaactgag ccgaaaatca aattttatat cggtgtaaaa ggaaactctt cagaagacgc
    40981 agctgaaaaa gtagccgctt atgaaacgat tatccaaaag atgacagaag aataaaaaaa
    41041 cttgagactg atggaaaaag tggtaaactt tagaagtcag tcttttttca agttggaggt
    41101 gaatggattg ccagacacgc aaaaaacaaa agtagagaaa gtcagccaac tattcaaaat
    41161 gttgagtgac cccactcgtc tcaaaattct gctgtatctg aaagacgggg aacaaaatgt
    41221 gacagcaatc atccaagcag tggaaatgga acaatctgct gtatcgcatc aactgcgttt
    41281 attaagagaa aaccatgtcg tcaagtccca tcgtgaggga aaagcgattt tgtatagtct
    41341 tgatgattct cacgtactgg atattttgaa ccagacactc aaacatgtgg aacatcaata
    41401 acgataggtg caaatagaga caaactggac tgtgtcaacg aatcttcttc gctgacacag
    41461 tccagttttt ttaaagaaaa attgttagtc tgtttcttcg gtggtttcag ttccataata
    41521 actatggtct tctaggtcta aagcattcaa aataagcgaa gctgtttctt caatagataa
    41581 agaagcgaca ttgattacta cgcagcccaa tttttcataa agatcattcg caaaatcgag
    41641 ttcagctcgt attttttcga tatcggaata agcagtatca ggatttaatc cgtaagagcg
    41701 catccgttct ttacggatat tgtttaaaat atctgggtta ttcgtcagtc ccacgatttt
    41761 tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca aaggtagatt
    41821 ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg aagttcggga
    41881 tacgcctagc aaaacgacat ctgcttctag aaacccacga ggatctttcc cgtcatcgta
    41941 ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca ataaatgaag
    42001 ggcgccaggt tgccgaatgg gcttgatacc agttaagcgc tcgatttctg atatcggcgg
    42061 agtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgcg tcatctcgat
    42121 caattcttga ttgatcaatg tgtgcaggac aagagctttt tcttctagtg catcttgcag
    42181 tgcctgcatc aatgttttct catctgtcac aaatgttctg cggaataaat tgaactcaac
    42241 gctaggatat tgggccatag tggcttgcgc tagttttgtc gcagtttctc cagcagagtc
    42301 ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata aaacctcact
    42361 tgcatttttt ttatgtttat tataccatac tctagaatgt gtacttcgta atcattttga
    42421 tttggaggaa taagtcatga aaaaacagac gttgatcgaa caatcactag aaacattgaa
    42481 aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt tagtgaagcg
    42541 caatcgctac gtatccgcta aagaagtttt tgactatatg aatcaggaat tcaaaggagt
    42601 aagttacgat acgatttatc gaaatctcca tgattttgca gaaatcgatt tactggaaga
    42661 aaccgattta aatggagaga tgaagtttcg ttttcattgc tgtcaagggg aaatcggcca
    42721 tcaccatcac tttatctgta cggtgtgcgg aacaacgaaa gaaatccata tgtgtccaat
    42781 gaattatttc gaagaacagc tcggtggatg tacgatcgaa gggcatagat tcgagatttt
    42841 cggaagatgt gaaaattgtc aataaggatt taaataaaat aacatttgat aattttaaaa
    42901 aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac aggacagtct
    42961 tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg gagggaggga
    43021 attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc tcttcgtcgc
    43081 ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg tgaattctac
    43141 gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg caaaaaattc
    43201 tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta aacgatgaca
    43261 tgaaaacagc gatgaaagcg aaagataagg aatctttaca agtcatccga atgatcaagt
    43321 catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa gaagaattaa
    43381 cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt gaagaagcag
    43441 gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt gatagtcgaa aaatacatgc
    43501 cggaacaact ttccgacgaa gaaattcgac aacttgttca agaagcaatc acgcaaactg
    43561 gtgcttcatc aatgaaagaa tttggcaaag tcatgggtgc gatcatgcct aaagtcaaag
    43621 gaaaagcaga tggaaaccaa gtaaatgcca ttgtgaaaga actattacaa tcttaatttt
    43681 atcatttaaa ctgcggacaa tcattgtccg cagttttttt gtgctcgtaa aaaagagatc
    43741 taaaaaatag ctagacgaaa gaataggatt ctttcatcta gctgtttttt tcctgtttaa
    43801 tatttcttat gttaaattgt gtttgatctg gttattcttc tggtagttca tttgcttctt
    43861 gtttataccc tgataggcga taggcgtaat agaagagtaa gctgccagca tacccaagta
    43921 acgttgggac catccagcca agtcccaatg cagcaaaagg aacgtgatga tgataaaatt
    43981 cgagcatgct tgctacaact ctttgttgac tcactaaagc aggcgaattt gccagcatat
    44041 ccaatacggc gggaacagca gcaaacaaca tcgttacttg atagacaaat ggtgtctttt
    44101 ggaaaaattt tgctgttaag gaaagcaaaa tcaacgcaag cgacaatgga tacaaaagca
    44161 tcaatactgg taaagaccat tggatgatat tgtccaaacc agcatttgca acgacaaaag
    44221 agacgaaagt cgttaaccgc agccatgtca tataactgac tttagggaac agtttatgga
    44281 aatcttgtgc aaaagaaaca acgagtccca tagcggtagt gaagacacct aatgtgacca
    44341 tcactcctaa gaagctagcg ccgaagttac ctaaataaaa attagtgatc tgagaaagtg
    44401 cagttccgcc attttctgat aaagacatct tattcaaact catcgcaccg agaagaacaa
    44461 gaccaaaata gatcatgact tctagcgcaa cgctcaggct accagctttt gctgttaatt
    44521 ctgtgacttt tttatctttg aaaccaaggt cacggactgc atggacaaaa gtgacactta
    44581 aggcaagcaa ggctactgca tcaaccgtgt tataaccttc taggacacca tttgttaaag
    44641 cgtttgtttg gtaagtatct gcagcagatt gatttagatt tcccattggg tggaataaag
    44701 aaacgacaaa aatgattgct aataatgcaa gaaacaagga gtttagatac tttccgacgt
    44761 atttgaccaa ccgtgtggga tggacagtaa ggaaataagc aagtaaaaag aaaagagcag
    44821 agaataaaaa catacctaat gaattatatt tttcaggcaa aaatggttta gcagccattt
    44881 caaaagcagt agcagctgtc cgtggtgtac cgaaaaatgg tccgatcgtc aaatgaacaa
    44941 ggattaagaa caaggcagca taccattttc cgacaggttt agctaagtca tacagaccat
    45001 cactttttgt cactactaca gctaagatag ctagtaatgg gaaaagagcg cctgagatgg
    45061 caaatcctac gccagcactg acccagtttg cgccagccat ttgacctaag tggacaggaa
    45121 agattagatt acctgacccg aaaaacatcc cgaagataag tgaactgacc atcaatagtt
    45181 gtttccatga ataagttttt gtttcttcca taaatttttc ctccaataat tatcattttg
    45241 attgacgata taaacgttgc agcaattagt aatgccacta atatcagatt ccatgaaaac
    45301 caccacctta aaatataaaa aaaagccctt ttccacttgg gaaaagggct ctagattcta
    45361 gaacggtacc accttttttc gcttaaaaaa gcttcttcat tcacggacgc tttttgataa
    45421 agttatccgc tagcctgata atggaggcag ccacaatgat ctacgttttc gacattgact
    45481 cggaaatgat gttcgtctat tcgatttatt gtcatttctc acttacatga ctcactggga
    45541 ataaatcgtt gaatagccta ctgttttcct cgacgctttg ctgtatgttt atttgttgac
    45601 gtaataatac acctgttttt tttgaaatgc aagaatattt taactatttt gttttctttt
    45661 tttgaaacaa gaaaaatacc tagagaatac ctctgaatga aacggaatat tttgttctcc
    45721 actttcatat aacttacatt ctcagattct ttttatcaaa acgagacctt tagcgaatgg
    45781 aaacagctat tttttaacgt ttcttggtac gtatggttaa acgactcttt tacaaccttt
    45841 tcttatttgc tatatagttt cttctgagta tggtaaaata ggaaagaatg aatcgaaaaa
    45901 aaggggatgt ctatttttga cggaagagtc tagttcttta gagattagat tgacagctgc
    45961 tgatgatgtc agcatgcttt taggtgcaca cgacaagcat ataaaactga tcgaggaaac
    46021 aacggaaaca accatccata cacgtggaga aatcattcag atcattggtg aacaatccca
    46081 gatttccttg gctgcctcag tcattcggac attacaggaa ttgatcaaac gaggaatcca
    46141 cgtaagtaca ccagatgtcg tgacggcatt gaaaatgggg caaaaaggta cattagatta
    46201 ttttgttgag atgtatgagg aagagatcgt caaagaccgt aatggaaaac ctatacgggt
    46261 aaaaaacagc ggtcaaaaga aatacgttga atcggtagcc aaacatgaca tcgtatttgg
    46321 tgtagggcca gccggaacag ggaaaacgtt tcttgccgta gtattagcaa ttgctgccct
    46381 aaaaaaaggc gaagtgcaaa aaatcatttt gacgcggccc gctgtagagg caggggaaaa
    46441 cctaggcttt ctacccgggg atctgaaaga aaaagtggat ccttatctgc ggcctgttta
    46501 tgatgcttta taccaaattt ttggattgga tcatacgaac cgattgatgg aacgaggcgt
    46561 aatcgaaatc gcaccattag cttacatgcg ggggcggaca ttggatgatg cattcgttat
    46621 cttggacgaa gcacaaaaca cgacagtcgc tcagatgaaa atgttcttga cccgtctcgg
    46681 ttatcaatca aaaatgattg tcaatgggga tacaagtcag atagacttgc ctcgagggac
    46741 gacaagtggt cttgttcatg cagaacgaac actaaagcaa atcaaaaaaa tcgattttgt
    46801 taattttgaa gcaagtgacg ttgttcggca ccctgtagtt gccgaaatca tcaaagctta
    46861 tgaaaaggcg gatcttcatc aagaataagc tttgcttagg aggataagca catgttgaat
    46921 aaaacactgc agaaactaca agaaaaactc ggaaaggcag cattgccttt ccttctgttc
    46981 ttgtttttta tcattgtctg cggcgttaca tttagtagtg tcaaacaaaa ggccaatgat
    47041 ttcagagaag gccaagtagc agaagagagt atccgtgcga ataaaacgat cgagaacacg
    47101 gaagaaaccg aacaaagaag aaaactggca gcagaagcag tgactccaga atatacttat
    47161 caaaaagatc tggcagatga ccagaacaat cggatcaagc aattattcga attgatcgat
    47221 aagacgaatg ataccatcaa caaatcttat aatgacaagg tagataaggc gaaggaagat
    47281 gaaaatatcc cgaaacctac tacagaagag cggattgcta cactgaagaa aaattttgaa
    47341 aacgtgaatg cagaaaacgt tgcgttttac caaaaactgc cgaacaattt ttattcgacg
    47401 atatttgaaa tgactgaaac ggaaattcac acggttaggg atgaaagttt gaagctcatt
    47461 gacgaacaga tgagtaaaca agttcgtgaa agcgaattgg aagcttttaa acaagaagca
    47521 gaagatcaga ttcagtattt gaatgtcaca acagagcagc aacaaatgat tcgctactta
    47581 gtggatcaag gcattgtagt caatgatgta ttgaatgaga aaaagacaga agaattaaaa
    47641 caatctgcac gagaagctgt acaaccagtg atgatcttcc aaggagaaat cattgttcgc
    47701 gaaggaaatc aaattgatgc ttcagcgatg aaaaaattgg aattactagg attgacgaat
    47761 caaacgactt cgattttccc actcattgcc atggttttag ctgttttgct tcaaatcggc
    47821 gtactgattt ataattcact tcaattccaa aatgcaggag aaagaacaaa atacgtgctt
    47881 ttctatgtga cagctatgtc agtcagcata gtgctgatga aatttttcca attattccaa
    47941 acggaacaag ctacttatat cccgctgttt tatcccgctg cttttgcacc acttgtttta
    48001 agctttttct tgaaccgaag agcagggatc atggctggta ttttccaagc agtatctgca
    48061 cttttcattt tttatgattc gattggcacg aactttctaa cgattatctt gatgtcttat
    48121 gttttctctg ggctcatggc aacgattgtc agacgcaaac ggatctcaga acaaggatta
    48181 tctgctgcca tgtgggtcat tatttttcct gtgatgatgg atgtcatttt gatcgtctat
    48241 caaggaatga gctttggtga tgcaacgact tggttaatgc ttgtctgcgg gttcactggt
    48301 gcactcttct cattcttgct gacgatcggt ctacacccat atattgaatt gatggtcaat
    48361 gatgacagtg taattgtatt aaatgaactc agcaatccaa atcatccttt attgaaacag
    48421 ttgctggaag aagcgccagg tacttatcat catagtatga tggtagctaa tctaagtgct
    48481 aacgctgtag cagaaatcgg cggacgttct ttgctaactc gagtagcttg ctattatcat
    48541 gatatcggca agatcaaaca tgctagcttt ttcgttgaga acttgccttc aggagcggaa
    48601 aacccgcaca atttcttatt gccagaagac agtaaacata ttatctttgg acatgtgaca
    48661 gatggagcca aaatattgga agattataat atgccgcaaa tggtgatcga tatttgcaaa
    48721 cagcatcatg gaaccacgct aatgcgttat ttctacatca aagcgaaaga acgcaatccg
    48781 gaagtaacag aagagcaatt ccgttatcct ggaccaaaac cgcaaagtcg tgaagcaggg
    48841 atcgtcaata ttgctgacag ttgtgaggct gcagtacggg caatggatca tccgacgagc
    48901 gaaaaaatcg aaagttttgt ccataatctt atcgaagagc gtatttccga tggtcaatta
    48961 gacgatagtg gactgacctt gaaagaaatc cgaaaagtgg aaaaatcatt gatcagcggt
    49021 ttaagtagca ccttccattc tcggatcaaa tatcctaaaa tgaaaacaga agttgagaag
    49081 ataaaggaag aacaagaaga atagaaagaa gcgatgtata atggatatta cctttatgga
    49141 tgaaacaaac caagtctcag aagagaaaat gaaagaaatc gatgacttgc tgcaatttgc
    49201 agcatcttat ttaaagctgc cagaggaaac agaaatgtct gttactttca tggacaatgc
    49261 ggcaatccaa gtaatcaatc gtgattaccg tggaaaagat gcaccgacag acgtcatcag
    49321 ttttgctctg gaagaagaag gcgaagacga aatcccagtg atttttgaag atggagagaa
    49381 tcctttgcct cgtgtactag gagacatcat gatttctatt gaacgtgctg ctgaacaagc
    49441 agaagcttac ggccattcat atgatcggga acttggcttt ttagcggtac atggattctt
    49501 gcatatcaac gggtatgacc atatgacacc agaagatgag aaagaaatgt ttggtttaca
    49561 gaaagagatc ttggatgcct atggactcaa aagataaaaa cgtagagaaa aacaaacatt
    49621 tcctgatgtc tgtggaattt gctgtccaag gagtcaaaac cgtttttgag gaagaacgca
    49681 atatgaggaa acatgttgcg ttcggtatcc ttgctttatt tatgggcgtg attttccagc
    49741 ttgatcggat agagtggtta tggctgcttt tagctgtttt tctcgtttgg atagtagaga
    49801 tcatcaatac tgtttttgaa aatgtagtgg acatgtttac ggattttcat tttcatccga
    49861 ttgggaaaaa aatcaaagat atggcagctg gagcagtttt agtgaccgct tgttttgctg
    49921 tcatcattgg gttgatttta tttgtcccta aaatatatca tttgttgttt aattaaaaag
    49981 gagaacgtat atgccagaac ataaatcagg gtttgtcgca atcgtcggcc gcccgaatgt
    50041 aggaaaatcc acattattga accgaatcgt gggtcaaaaa attgccatta tgagtgacaa
    50101 ggcacagaca actagaaata aaatccaagg agtatatact acaccagaag ctcagctgat
    50161 ttttatcgat acaccaggaa tccataaacc aaagcatcgt ctaggagatt ttatggtaga
    50221 agcagcttac agtgcgttga aagaagtgga tgcagtctta tttatgatca gtgctgatca
    50281 aaaacgcggc cggggagacg attttattat tgaacgtcta aaaaacgtgc attctccagt
    50341 ttatctgatt atcaataaaa tcgataagat ccatccagac gagttgctgg gaatcatcga
    50401 agattattcg acgcagatgc cttttgcaca agtgattccg atctctgcta cagaaggaaa
    50461 caatgtcgaa cgcttaatgg atgtattagt cagtgaaatg ccagagggcc cacaatattt
    50521 tccagatgat caagtgactg atcatcctga atactttatc gtttctgaat tgatccgtga
    50581 gaaagtcttg tttttaacac gagacgaagt gcctcattct gtcgcagtcg ttgttgattc
    50641 gatgaagcga aacgagaatg ataaaatcca tatccaggcc acgatcatcg tcgaaagaga
    50701 tagccaaaaa ggaatcatta tcggcaaagg cggaaaaatg ctgaagcaag taggaacgaa
    50761 agcaagaaag gatatcgaaa atcttttagg agacaaagta tttctagaac tttgggtcaa
    50821 agtccaaaaa gactggcgag acaaaaaagt ctatctgcaa gactttggct accgaaaaga
    50881 cgaatactga tattctgaac ttcttatagg ttaagtatag aaataaactg gataaaaatc
    50941 cgcacgttga gaaaaacggc ggaagatcga aacataaata atctggatcg gcagaatctg
    51001 cttttccaga tttatttttt aggagggaga atatgagcca gcttgcagaa tcgaaaggct
    51061 tgattttatc tagccgtaat tacaaagaaa aagataaatt agtcaaaatc tttacggaat
    51121 cttctggcaa aatgatgttt tatatcaaag gaatccatcg gcaaaatcag ccattagctc
    51181 cggctttgct cccctttacc gaagcagtct atatcggtaa ttttcgaagt gaaggtttgt
    51241 cgtttttaag cagtgtcaaa gacgtccatg cgtttcggaa catccaagaa gatatctttt
    51301 tgagcggtta tggtacctat ttgctaaatc tagtagatgc agcaatcgaa gatcatcaat
    51361 atgacccgca tttattccaa tttacgcaac aagctctgca gcggatggat caaggagatg
    51421 atgcagaaat catcacgaat atctttgaag ttcaaatact gcaacgcttc ggaatcgcgc
    51481 caatttggac gcactgtgtc gtatgcgggg aaacaaaagg aaaatttgac tattcttcca
    51541 aatctggcgg tgtgatatgc gagaaacact ggccaatgga cgaacaccgt tatcatgcag
    51601 atcctcgcgc tgtttatttc gttcgaatgt tttcggctat ttcttatgat aagatcagtg
    51661 gaatccactt gaaagaagaa acgaagcagg caatccgtga cctcattgac cagttgtatg
    51721 atgaatatgt cggtattcat ctaaaaagca aaaagttcat tgatcaaatg aagtcttggg
    51781 cacacgtatt aaagacgcca gagaaaaata tgcctgaaaa agagatacct gaaaaagaag
    51841 aataaatgaa agatacaaca aaaaaatcaa aactatttga caaatacagg gacttccgtc
    51901 tattattaac cgtagattaa atagatcgtt cactgagagg atctattata taaacaagaa
    51961 aactgcaaaa tgaatacgta cattgaccaa gatcagtaaa aaacacgtgg tttagcgagt
    52021 tcgggagagt ggaagccgga cccaaacgat ttttgaagag gcacttgcga gtacgataaa
    52081 tgaagctgcc gaatctttat tcggaagtag ggtggaaccg cgaaattatt cgtccctatg
    52141 tctgtaatca gacataggga ctttttgtcg ttttctttcc tgtattctta ttgacagcaa
    52201 cgcttaaaaa ttgaaagggg caaaacagat gagtaaaaaa ttgaccgtcc aagatatgat
    52261 tttaacatta caaaaatttt ggtcatcaaa cggctgcatg ctgatgcaag cttatgacac
    52321 agaaaaagga gcaggtacga tgagcccata tacattctta cgggcaatcg gaccggaacc
    52381 atggaatgct gcttatgtag agccatcccg ccgaccagct gacgggcgtt atggagaaaa
    52441 cccaaaccgt ttgtatcagc atcatcaatt tcaagtcgta atgaaacctt cacctgaaaa
    52501 tatccaagag ctttatttag aaagcttgcg tcttttagga attgatccat tagaacacga
    52561 tattcgtttt gttgaagaca actgggagaa cccatcgatg gggtgtgccg gtgtgggttg
    52621 ggaagtatgg cttgatggga tggagatcac acaattcacg tacttccagc aagtcggagg
    52681 actaccttgt aaaccagtta cttctgagat cacttatgga ctagaacgtc ttgcttcata
    52741 tattcaagaa gttgaaagtg tctatgatct ggaatgggct gatggtgtga aatatggcga
    52801 gatcttcaaa caaccagaat atgaacattc caaatattct tttgaagtaa gtgaccaaga
    52861 cttgctacta agcaactttg accgttttga aaaagaagct aaacgctgta tcgatgaaaa
    52921 tcttgttcat ccagcgtatg attatatttt gaaatgcagc catacattca atttattaga
    52981 tgctcgcggt gcagtatccg taacagaacg agcaggttat ctttcccgta tccgcaacat
    53041 ggctcgtgct gtagccaaaa tctttgtggc agaacgcgaa aaattaggat tccctttatt
    53101 gaataaaaac acagcaaatc cagaaaacat gaaggaggct gaataagatg gcaaaagact
    53161 tattacttga aatcgggctg gaagaaatgc ctgctcatgt tgtcacacca agcatgaaac
    53221 aattagaaga aaaaacagca aaatttttag atgaaaacca attgacttat gatacgatcg
    53281 aaacgttttc tacaccgcgc cgcttagcaa tcaaagtaac aaacattcca gaacgtcaag
    53341 cagacagcga agaagaagtc aaaggaccag ccaaaaaaat tgctttggac gcagatggaa
    53401 actggacgaa agcagctgaa ggatttgtcc gcggacaagg tctgacgaca gatgctatca
    53461 cttttagaga attaaaaggc gtggaatatg tctatgtaac gaaatacact cacggcaaag
    53521 aagcaaaaga agtattgaca ggtttagcag atgtcattaa aagcctcact ttcccagtaa
    53581 cgatgcactg ggcagaatac gattttgaat atattcgccc tattcactgg atcgtggcat
    53641 tgttagatga cgaaataatc ccgtttgaga tcttggatgt caaaacagac agagcttcaa
    53701 gaggtcatcg tttcttaggc gaagatgtga catttacgca tgcaaaagaa tacgaagaaa
    53761 aattgaatga acaatttgtt attgcttcac cgcaaaaaag aaaagaaatg atcatcacac
    53821 aagcaaacga attagccgaa aaaaatcact ggatcattga tctagatgaa gatttactag
    53881 aagaagtcaa caatttagtc gaatatccaa ctgtcttttc tggcgacttc gaagaaaaat
    53941 atctatctgt tcctgaagaa gtactagtca cttctatgaa agaacatcaa cgttatttcg
    54001 aagtacggaa cgatcaaggt atgttgcttc ctcattttat ttctgctcgt aatggcgatc
    54061 gggtaaaatt ggaaaatgtg gtacgaggca atcaaaaagt attgacggct cgtttagaag
    54121 atgcggaatt cttttataat gaagacaaga aagcttcaat cgaccaatac gttgaaaaat
    54181 taaaaactgt tactttccat gaaaaaatcg gtagcatcta tgagaaaatg caacgggtac
    54241 aagtcatcgc tcgcatcata ggcgaacaag taggactatc tgaggaagaa ttgatggatc
    54301 tgcaacgcgc tgctgaaatc tataaatttg atctagtaac caacatggta ggagaattcc
    54361 ctgaactaca agggatcatg ggagaaaaat atgctctgct gaaaggcgag aaaccagccg
    54421 tagcaacagc gatccgtgaa cattacatgc ctatatccag tgaaggagat ttacctgaga
    54481 ctacagtagg ttctgtttta gcgattgctg ataaattaga tagtatcttt tctttctttg
    54541 cagttgggat gatcccaagt ggctcaaacg atccatatgc tttgcgccgt caagcatatg
    54601 gtgtcgttcg aatcatcgaa tcgaaaaaat gggcctttcc tttgtctgtt ctgcaagaaa
    54661 cgattagtga agttatcagt aaagatacgg atcgttttgg cattggcttg tcagcgggac
    54721 aacaacaggt gatcgacttt atcaaaggcc gtctgcgcca attattgaca acgaagaata
    54781 tccgtcatga tgtcatcgaa gctgttttga atgcagaaca aaaagatctg acaaaagtat
    54841 ttgcagcagc acaattgttc aagcagcatc ttgcagatga agacttcaaa ccatcgatgg
    54901 aagctttgac acgtgtcgtg aacttagcga aaaaagcgga gcttgaaaaa caatcagaag
    54961 tagaccctga actgttcgaa aacgaagcgg agaaagaact gcacaaagca gtagaggcaa
    55021 tgaaaacaag ctttgctgac caaacgatca acgaaaatta tgatcagctt gtaagccttc
    55081 gcccactgat cgaaaattat tttgatcaga cgatggtcat ggcagaagaa ccagcagtac
    55141 gtaagaaccg attaacgcaa ttaagtcaaa ttgctgccat ggctttgtca ctagcaagtt
    55201 tagatcaaat catcacaaaa taatttctaa tatgtaaata aaccagagaa caggcacaaa
    55261 ataggcta
//
LOCUS       DACCXW010000005        46532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCXW010000005 DACCXW010000000
VERSION     DACCXW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.005"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            18..899
                     /locus_tag="IVY71_000220"
     CDS             18..899
                     /locus_tag="IVY71_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984299.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            912..2522
                     /locus_tag="IVY71_000221"
     CDS             912..2522
                     /locus_tag="IVY71_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7984300.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2599..3258)
                     /locus_tag="IVY71_000222"
     CDS             complement(2599..3258)
                     /locus_tag="IVY71_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7984301.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3541..4206)
                     /locus_tag="IVY71_000223"
     CDS             complement(3541..4206)
                     /locus_tag="IVY71_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984302.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="IVY71_000224"
     CDS             complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="IVY71_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7984303.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4986..5969)
                     /gene="dhaK"
                     /locus_tag="IVY71_000225"
     CDS             complement(4986..5969)
                     /gene="dhaK"
                     /locus_tag="IVY71_000225"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7984304.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="IVY71_000226"
     CDS             complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="IVY71_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7984305.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6520..6840
                     /locus_tag="IVY71_000227"
     CDS             6520..6840
                     /locus_tag="IVY71_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7984306.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6952..7821)
                     /locus_tag="IVY71_000228"
     CDS             complement(6952..7821)
                     /locus_tag="IVY71_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7984307.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7917..9221)
                     /locus_tag="IVY71_000229"
     CDS             complement(7917..9221)
                     /locus_tag="IVY71_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7984308.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9218..9727)
                     /locus_tag="IVY71_000230"
     CDS             complement(9218..9727)
                     /locus_tag="IVY71_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984309.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9902..12037)
                     /locus_tag="IVY71_000231"
     CDS             complement(9902..12037)
                     /locus_tag="IVY71_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7984310.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12120..13775)
                     /locus_tag="IVY71_000232"
     CDS             complement(12120..13775)
                     /locus_tag="IVY71_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7984311.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13777..14091)
                     /locus_tag="IVY71_000233"
     CDS             complement(13777..14091)
                     /locus_tag="IVY71_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7984312.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14261..14521
                     /locus_tag="IVY71_000234"
     CDS             14261..14521
                     /locus_tag="IVY71_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7984313.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14535..14849
                     /locus_tag="IVY71_000235"
     CDS             14535..14849
                     /locus_tag="IVY71_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7984314.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15579..16133)
                     /locus_tag="IVY71_000236"
     CDS             complement(15579..16133)
                     /locus_tag="IVY71_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7984315.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16259..18188)
                     /locus_tag="IVY71_000237"
                     /pseudo
     CDS             complement(16259..18188)
                     /locus_tag="IVY71_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18494..19210)
                     /locus_tag="IVY71_000238"
     CDS             complement(18494..19210)
                     /locus_tag="IVY71_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7984316.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19211..21034)
                     /gene="glpO"
                     /locus_tag="IVY71_000239"
     CDS             complement(19211..21034)
                     /gene="glpO"
                     /locus_tag="IVY71_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7984317.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21048..22544)
                     /gene="glpK"
                     /locus_tag="IVY71_000240"
     CDS             complement(21048..22544)
                     /gene="glpK"
                     /locus_tag="IVY71_000240"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7984318.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22680..24140)
                     /locus_tag="IVY71_000241"
     CDS             complement(22680..24140)
                     /locus_tag="IVY71_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7984319.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24130..25425)
                     /locus_tag="IVY71_000242"
     CDS             complement(24130..25425)
                     /locus_tag="IVY71_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7984320.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25757..26434)
                     /locus_tag="IVY71_000243"
     CDS             complement(25757..26434)
                     /locus_tag="IVY71_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7984321.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26464..27555)
                     /locus_tag="IVY71_000244"
     CDS             complement(26464..27555)
                     /locus_tag="IVY71_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7984322.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27656..28090)
                     /locus_tag="IVY71_000245"
     CDS             complement(27656..28090)
                     /locus_tag="IVY71_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7984323.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28674..30131)
                     /gene="ascB"
                     /locus_tag="IVY71_000246"
     CDS             complement(28674..30131)
                     /gene="ascB"
                     /locus_tag="IVY71_000246"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7984324.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30128..32023)
                     /locus_tag="IVY71_000247"
     CDS             complement(30128..32023)
                     /locus_tag="IVY71_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7984325.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32081..33856)
                     /locus_tag="IVY71_000248"
     CDS             complement(32081..33856)
                     /locus_tag="IVY71_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7984326.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33906..35402)
                     /locus_tag="IVY71_000249"
     CDS             complement(33906..35402)
                     /locus_tag="IVY71_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7984327.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35596..37218)
                     /locus_tag="IVY71_000250"
     CDS             complement(35596..37218)
                     /locus_tag="IVY71_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7984328.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37546..38859)
                     /locus_tag="IVY71_000251"
     CDS             complement(37546..38859)
                     /locus_tag="IVY71_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7984329.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39104..39328)
                     /locus_tag="IVY71_000252"
     CDS             complement(39104..39328)
                     /locus_tag="IVY71_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984330.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39348..39716)
                     /locus_tag="IVY71_000253"
     CDS             complement(39348..39716)
                     /locus_tag="IVY71_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7984331.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(39912..40622)
                     /locus_tag="IVY71_000254"
     CDS             complement(39912..40622)
                     /locus_tag="IVY71_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984332.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40640..41137)
                     /locus_tag="IVY71_000255"
     CDS             complement(40640..41137)
                     /locus_tag="IVY71_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7984333.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41174..41626)
                     /locus_tag="IVY71_000256"
     CDS             complement(41174..41626)
                     /locus_tag="IVY71_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7984334.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41761..42366)
                     /locus_tag="IVY71_000257"
     CDS             complement(41761..42366)
                     /locus_tag="IVY71_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7984335.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42816..43436)
                     /locus_tag="IVY71_000258"
     CDS             complement(42816..43436)
                     /locus_tag="IVY71_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7984336.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43438..44307)
                     /locus_tag="IVY71_000259"
     CDS             complement(43438..44307)
                     /locus_tag="IVY71_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7984337.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44330..44737)
                     /locus_tag="IVY71_000260"
     CDS             complement(44330..44737)
                     /locus_tag="IVY71_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7984338.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44756..46045)
                     /locus_tag="IVY71_000261"
     CDS             complement(44756..46045)
                     /locus_tag="IVY71_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7984339.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            46103..>46532
                     /locus_tag="IVY71_000262"
     CDS             46103..>46532
                     /locus_tag="IVY71_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7984340.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        13105 a         9900 c         7984 g        15543 t
ORIGIN      
        1 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
       61 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
      121 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
      181 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
      241 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
      301 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
      361 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
      421 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
      481 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
      541 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
      601 gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa ggaacactcg
      661 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
      721 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
      781 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
      841 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
      901 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
      961 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
     1021 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
     1081 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
     1141 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
     1201 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
     1261 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
     1321 gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt tttttccaag
     1381 cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
     1441 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
     1501 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
     1561 ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta
     1621 caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
     1681 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
     1741 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
     1801 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
     1861 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
     1921 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc ttgatttgtc
     1981 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
     2041 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
     2101 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
     2161 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
     2221 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
     2281 ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
     2341 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
     2401 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
     2461 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
     2521 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
     2581 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
     2641 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
     2701 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
     2761 atccttccct gtcgctttag ctaagtcaag cagatagaga gcgactcgtt cttctacttt
     2821 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
     2881 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
     2941 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
     3001 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
     3061 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
     3121 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
     3181 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
     3241 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
     3301 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
     3361 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
     3421 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
     3481 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
     3541 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
     3601 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
     3661 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
     3721 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
     3781 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
     3841 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
     3901 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
     3961 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
     4021 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
     4081 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
     4141 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
     4201 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
     4261 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
     4321 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
     4381 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
     4441 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     4501 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     4561 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     4621 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     4681 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     4741 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     4801 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     4861 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     4921 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     4981 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     5041 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     5101 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     5161 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     5221 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     5281 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     5341 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     5401 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     5461 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     5521 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     5581 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     5641 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     5701 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     5761 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     5821 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     5881 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     5941 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     6001 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     6061 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     6121 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     6181 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     6241 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     6301 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     6361 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     6421 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     6481 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     6541 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     6601 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     6661 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     6721 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     6781 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     6841 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     6901 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     6961 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     7021 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     7081 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     7141 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     7201 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     7261 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     7321 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     7381 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     7441 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     7501 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     7561 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     7621 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     7681 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     7741 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     7801 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     7861 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     7921 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     7981 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     8041 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     8101 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     8161 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     8221 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     8281 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     8341 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
     8401 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     8461 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     8521 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     8581 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     8641 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     8701 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     8761 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     8821 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     8881 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     8941 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
     9001 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     9061 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     9121 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
     9181 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
     9241 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
     9301 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
     9361 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
     9421 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
     9481 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
     9541 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
     9601 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
     9661 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
     9721 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
     9781 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
     9841 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
     9901 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
     9961 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
    10021 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
    10081 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
    10141 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
    10201 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
    10261 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
    10321 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
    10381 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
    10441 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
    10501 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
    10561 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
    10621 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
    10681 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
    10741 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
    10801 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
    10861 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
    10921 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
    10981 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
    11041 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
    11101 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
    11161 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
    11221 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
    11281 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
    11341 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
    11401 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
    11461 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
    11521 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
    11581 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
    11641 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
    11701 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
    11761 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
    11821 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
    11881 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
    11941 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
    12001 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
    12061 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
    12121 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
    12181 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
    12241 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
    12301 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
    12361 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
    12421 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
    12481 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
    12541 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
    12601 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
    12661 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
    12721 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
    12781 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
    12841 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
    12901 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
    12961 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
    13021 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
    13081 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
    13141 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
    13201 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
    13261 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
    13321 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
    13381 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
    13441 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    13501 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    13561 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    13621 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    13681 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    13741 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    13801 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    13861 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    13921 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    13981 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    14041 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    14101 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    14161 tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    14221 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    14281 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    14341 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    14401 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    14461 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    14521 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    14581 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    14641 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    14701 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    14761 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    14821 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    14881 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    14941 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    15001 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    15061 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    15121 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga
    15181 agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt
    15241 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa
    15301 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    15361 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    15421 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
    15481 cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt
    15541 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
    15601 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
    15661 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
    15721 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
    15781 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
    15841 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
    15901 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
    15961 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
    16021 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
    16081 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
    16141 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
    16201 atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc
    16261 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
    16321 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
    16381 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
    16441 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
    16501 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
    16561 gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg
    16621 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
    16681 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
    16741 ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    16801 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    16861 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    16921 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    16981 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    17041 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    17101 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    17161 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    17221 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    17281 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    17341 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    17401 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    17461 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    17521 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    17581 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    17641 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    17701 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    17761 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    17821 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    17881 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    17941 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    18001 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    18061 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    18121 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    18181 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    18241 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    18301 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    18361 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    18421 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    18481 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    18541 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    18601 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    18661 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    18721 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    18781 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    18841 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    18901 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    18961 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    19021 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    19081 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    19141 catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat
    19201 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    19261 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    19321 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    19381 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    19441 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    19501 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    19561 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    19621 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    19681 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    19741 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    19801 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    19861 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    19921 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    19981 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    20041 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    20101 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    20161 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    20221 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    20281 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    20341 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    20401 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    20461 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    20521 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    20581 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    20641 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    20701 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    20761 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    20821 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    20881 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    20941 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    21001 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    21061 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    21121 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    21181 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    21241 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    21301 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    21361 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    21421 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    21481 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    21541 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    21601 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    21661 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    21721 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    21781 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    21841 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    21901 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    21961 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    22021 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    22081 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    22141 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    22201 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    22261 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    22321 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    22381 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    22441 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    22501 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    22561 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    22621 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    22681 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    22741 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    22801 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    22861 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    22921 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    22981 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    23041 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    23101 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    23161 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    23221 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    23281 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    23341 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    23401 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    23461 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    23521 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    23581 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    23641 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    23701 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    23761 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    23821 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    23881 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    23941 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    24001 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    24061 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    24121 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    24181 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    24241 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    24301 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    24361 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    24421 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    24481 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    24541 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    24601 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    24661 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    24721 acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    24781 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    24841 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    24901 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    24961 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    25021 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    25081 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    25141 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    25201 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    25261 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    25321 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    25381 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    25441 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    25501 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    25561 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    25621 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    25681 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    25741 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    25801 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    25861 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    25921 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    25981 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    26041 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    26101 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    26161 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    26221 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    26281 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    26341 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    26401 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    26461 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    26521 atatgctttt tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    26581 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    26641 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    26701 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    26761 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    26821 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    26881 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    26941 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    27001 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    27061 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    27121 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    27181 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    27241 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    27301 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    27361 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    27421 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    27481 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    27541 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    27601 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    27661 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    27721 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    27781 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    27841 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    27901 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    27961 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    28021 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    28081 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    28141 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    28201 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    28261 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    28321 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    28381 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    28441 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    28501 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    28561 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    28621 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    28681 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    28741 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    28801 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    28861 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg
    28921 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    28981 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    29041 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    29101 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    29161 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    29221 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    29281 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    29341 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    29401 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    29461 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    29521 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    29581 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    29641 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    29701 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    29761 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    29821 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    29881 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    29941 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    30001 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    30061 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    30121 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    30181 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    30241 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    30301 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    30361 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    30421 ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa
    30481 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    30541 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    30601 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    30661 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    30721 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    30781 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    30841 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    30901 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    30961 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    31021 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    31081 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    31141 ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac
    31201 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    31261 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    31321 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    31381 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    31441 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    31501 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    31561 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    31621 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    31681 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    31741 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    31801 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    31861 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    31921 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    31981 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc
    32041 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    32101 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    32161 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    32221 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    32281 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    32341 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    32401 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    32461 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    32521 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    32581 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    32641 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    32701 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    32761 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    32821 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    32881 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    32941 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    33001 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    33061 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    33121 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    33181 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    33241 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa
    33301 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    33361 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    33421 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    33481 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    33541 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    33601 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    33661 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    33721 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    33781 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    33841 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    33901 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    33961 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    34021 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    34081 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    34141 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    34201 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    34261 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    34321 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    34381 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    34441 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    34501 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    34561 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    34621 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    34681 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    34741 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    34801 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    34861 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    34921 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    34981 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    35041 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    35101 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    35161 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    35221 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    35281 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    35341 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    35401 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    35461 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    35521 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    35581 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    35641 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    35701 tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    35761 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    35821 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    35881 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    35941 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    36001 cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa
    36061 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    36121 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    36181 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    36241 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    36301 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    36361 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    36421 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    36481 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    36541 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    36601 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    36661 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    36721 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    36781 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    36841 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    36901 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    36961 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    37021 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    37081 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    37141 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    37201 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    37261 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    37321 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    37381 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    37441 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    37501 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    37561 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    37621 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    37681 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    37741 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    37801 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    37861 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    37921 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    37981 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    38041 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    38101 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    38161 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    38221 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
    38281 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    38341 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    38401 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    38461 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    38521 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    38581 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    38641 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    38701 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    38761 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    38821 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    38881 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    38941 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    39001 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    39061 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    39121 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    39181 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    39241 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    39301 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    39361 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    39421 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    39481 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    39541 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    39601 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    39661 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    39721 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    39781 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    39841 tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta
    39901 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    39961 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    40021 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    40081 agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    40141 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    40201 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    40261 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    40321 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    40381 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    40441 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    40501 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    40561 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    40621 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    40681 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    40741 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    40801 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    40861 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    40921 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    40981 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    41041 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    41101 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    41161 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    41221 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    41281 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    41341 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    41401 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    41461 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    41521 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    41581 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    41641 attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa
    41701 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    41761 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    41821 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    41881 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    41941 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    42001 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    42061 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    42121 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    42181 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    42241 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    42301 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    42361 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    42421 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    42481 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    42541 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
    42601 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    42661 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
    42721 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
    42781 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    42841 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    42901 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    42961 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    43021 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttgc
    43081 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    43141 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    43201 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    43261 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    43321 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    43381 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    43441 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    43501 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
    43561 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    43621 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    43681 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    43741 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    43801 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    43861 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    43921 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
    43981 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
    44041 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
    44101 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    44161 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
    44221 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    44281 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    44341 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    44401 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    44461 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
    44521 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
    44581 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    44641 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    44701 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    44761 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    44821 catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact agcaaagcgg
    44881 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    44941 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    45001 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    45061 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
    45121 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    45181 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
    45241 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    45301 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    45361 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    45421 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    45481 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    45541 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    45601 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    45661 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    45721 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    45781 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    45841 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
    45901 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    45961 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    46021 cgatgataaa attgataacg cccatgtttt gatgtgcacc ccaaaagtta gactagaaat
    46081 ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga aaaaagtagt
    46141 ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat atcaaataaa
    46201 tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg aaggcttggt
    46261 tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat atatggaaac
    46321 aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa atagaactct
    46381 aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga gccaaaaaag
    46441 gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta aagatgagtc
    46501 tcttgaagaa gaagttattc gtttaagagc ag
//
LOCUS       DACCXW010000006        45767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCXW010000006 DACCXW010000000
VERSION     DACCXW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.006"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(89..1192)
                     /locus_tag="IVY71_000263"
     CDS             complement(89..1192)
                     /locus_tag="IVY71_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7984341.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1414..1884)
                     /locus_tag="IVY71_000264"
     CDS             complement(1414..1884)
                     /locus_tag="IVY71_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7984342.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2217..3857
                     /locus_tag="IVY71_000265"
     CDS             2217..3857
                     /locus_tag="IVY71_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7984343.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4011..5333
                     /locus_tag="IVY71_000266"
     CDS             4011..5333
                     /locus_tag="IVY71_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7984344.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5395..5826)
                     /locus_tag="IVY71_000267"
     CDS             complement(5395..5826)
                     /locus_tag="IVY71_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7984345.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5890..6726)
                     /locus_tag="IVY71_000268"
     CDS             complement(5890..6726)
                     /locus_tag="IVY71_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7984346.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(7468..8094)
                     /locus_tag="IVY71_000269"
     CDS             complement(7468..8094)
                     /locus_tag="IVY71_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984347.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8389..8724)
                     /locus_tag="IVY71_000270"
     CDS             complement(8389..8724)
                     /locus_tag="IVY71_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7984348.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(8711..8995)
                     /locus_tag="IVY71_000271"
     CDS             complement(8711..8995)
                     /locus_tag="IVY71_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP7984349.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9051..10574)
                     /locus_tag="IVY71_000272"
     CDS             complement(9051..10574)
                     /locus_tag="IVY71_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7984350.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10567..11742)
                     /locus_tag="IVY71_000273"
     CDS             complement(10567..11742)
                     /locus_tag="IVY71_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7984351.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(11746..11931)
                     /locus_tag="IVY71_000274"
     CDS             complement(11746..11931)
                     /locus_tag="IVY71_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984352.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(11897..13591)
                     /locus_tag="IVY71_000275"
     CDS             complement(11897..13591)
                     /locus_tag="IVY71_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7984353.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13588..14061)
                     /locus_tag="IVY71_000276"
     CDS             complement(13588..14061)
                     /locus_tag="IVY71_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP7984354.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14130..14285)
                     /locus_tag="IVY71_000277"
     CDS             complement(14130..14285)
                     /locus_tag="IVY71_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP7984355.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14419..14799)
                     /locus_tag="IVY71_000278"
     CDS             complement(14419..14799)
                     /locus_tag="IVY71_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7984356.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(14803..15018)
                     /locus_tag="IVY71_000279"
     CDS             complement(14803..15018)
                     /locus_tag="IVY71_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984357.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(15021..15428)
                     /locus_tag="IVY71_000280"
     CDS             complement(15021..15428)
                     /locus_tag="IVY71_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP7984358.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(15694..17169)
                     /locus_tag="IVY71_000281"
     CDS             complement(15694..17169)
                     /locus_tag="IVY71_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP7984359.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17159..18007)
                     /locus_tag="IVY71_000282"
     CDS             complement(17159..18007)
                     /locus_tag="IVY71_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP7984360.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18044..18406)
                     /locus_tag="IVY71_000283"
     CDS             complement(18044..18406)
                     /locus_tag="IVY71_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984361.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18706..19330)
                     /locus_tag="IVY71_000284"
                     /pseudo
     CDS             complement(18706..19330)
                     /locus_tag="IVY71_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19327..19638)
                     /locus_tag="IVY71_000285"
     CDS             complement(19327..19638)
                     /locus_tag="IVY71_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984362.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(19682..19975)
                     /locus_tag="IVY71_000286"
     CDS             complement(19682..19975)
                     /locus_tag="IVY71_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984363.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20169..20816
                     /locus_tag="IVY71_000287"
     CDS             20169..20816
                     /locus_tag="IVY71_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7984364.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            20877..22022
                     /locus_tag="IVY71_000288"
     CDS             20877..22022
                     /locus_tag="IVY71_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7984365.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22103..22408)
                     /locus_tag="IVY71_000289"
     CDS             complement(22103..22408)
                     /locus_tag="IVY71_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7984366.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22433..22864)
                     /gene="ruvX"
                     /locus_tag="IVY71_000290"
     CDS             complement(22433..22864)
                     /gene="ruvX"
                     /locus_tag="IVY71_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7984367.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(22861..23127)
                     /locus_tag="IVY71_000291"
     CDS             complement(22861..23127)
                     /locus_tag="IVY71_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7984368.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23382..24107)
                     /locus_tag="IVY71_000292"
     CDS             complement(23382..24107)
                     /locus_tag="IVY71_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7984369.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24468..24538)
                     /locus_tag="IVY71_000293"
     tRNA            complement(24468..24538)
                     /locus_tag="IVY71_000293"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24504..24506),aa:Gly,seq:ccc)
     gene            24655..25407
                     /locus_tag="IVY71_000294"
     CDS             24655..25407
                     /locus_tag="IVY71_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7984370.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25423..25902
                     /locus_tag="IVY71_000295"
     CDS             25423..25902
                     /locus_tag="IVY71_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7984371.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26153..27733)
                     /locus_tag="IVY71_000296"
     CDS             complement(26153..27733)
                     /locus_tag="IVY71_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7984372.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(27726..28469)
                     /locus_tag="IVY71_000297"
     CDS             complement(27726..28469)
                     /locus_tag="IVY71_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984373.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(28645..29421)
                     /locus_tag="IVY71_000298"
     CDS             complement(28645..29421)
                     /locus_tag="IVY71_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7984374.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(29669..30505)
                     /locus_tag="IVY71_000299"
     CDS             complement(29669..30505)
                     /locus_tag="IVY71_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7984375.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(30516..31445)
                     /locus_tag="IVY71_000300"
     CDS             complement(30516..31445)
                     /locus_tag="IVY71_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7984376.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            31637..31990
                     /locus_tag="IVY71_000301"
     CDS             31637..31990
                     /locus_tag="IVY71_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7984377.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(32064..32720)
                     /locus_tag="IVY71_000302"
     CDS             complement(32064..32720)
                     /locus_tag="IVY71_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7984378.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            32946..34880
                     /locus_tag="IVY71_000303"
     CDS             32946..34880
                     /locus_tag="IVY71_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7984379.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(34966..36189)
                     /locus_tag="IVY71_000304"
     CDS             complement(34966..36189)
                     /locus_tag="IVY71_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7984380.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(36183..38081)
                     /locus_tag="IVY71_000305"
     CDS             complement(36183..38081)
                     /locus_tag="IVY71_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7984381.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(38075..39193)
                     /locus_tag="IVY71_000306"
     CDS             complement(38075..39193)
                     /locus_tag="IVY71_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7984382.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(39276..40109)
                     /locus_tag="IVY71_000307"
     CDS             complement(39276..40109)
                     /locus_tag="IVY71_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7984383.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(40102..41019)
                     /locus_tag="IVY71_000308"
     CDS             complement(40102..41019)
                     /locus_tag="IVY71_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7984384.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(41042..41221)
                     /locus_tag="IVY71_000309"
     CDS             complement(41042..41221)
                     /locus_tag="IVY71_000309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984385.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(41246..42370)
                     /locus_tag="IVY71_000310"
     CDS             complement(41246..42370)
                     /locus_tag="IVY71_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7984386.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            42553..43461
                     /locus_tag="IVY71_000311"
     CDS             42553..43461
                     /locus_tag="IVY71_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7984387.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            43602..45215
                     /locus_tag="IVY71_000312"
     CDS             43602..45215
                     /locus_tag="IVY71_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7984388.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        13818 a         9173 c         7629 g        15147 t
ORIGIN      
        1 aaattattct attagtgaca tccttgaact ataaaaatag gagtacggca tcatctgccg
       61 cactcctatt tttttcgtca tttgtttctt aaataaaacg attctcacgc aattttattt
      121 ttttgatatc ttctactgtt tgtgttccag ctaattgcat gaccatttcc aattctgttt
      181 taaagaaatc aaacacttga tggactcctg tacttcctcc taaagacaat ccataaataa
      241 cgggtctgcc aatagcaaca agatctgcac ctgatgcgat tgctttgaaa acatgctgtc
      301 cgcgtcgtac accgctgtca aacacgattg gtacacgttt atctactgct tctgccacat
      361 attgcaacga atcaaacgct gctggaccac catctagttg acggccacca tgattagata
      421 cccagattcc gccagcacca gattctaatg aacgatagac atcttcttct gactggacac
      481 ctttcacata gactggcaaa tcagaatgag cagcaataaa ttctacatct tttggactaa
      541 gcttctgttt ggaagatttg tacacagcat ccatcgtttg gcctactcct gattgataag
      601 cctgaacgat cggcattggc agtgggaagg taaacccatt tctgcggtct gtttcacggt
      661 ttccgccaac tgttgcatcc gctgtcaata cgattgcttt tgctccatta cgcttggcca
      721 cttcaagaat atccaaattg atcccatcat ctttgctcat ataaaattga aaccattgcg
      781 gtgctttctc cccgcctgct tctcttatct cttctaatgt acaagaagcg taagaacttg
      841 ctgtatagat cgtcccaaaa tctgctacac cttttgcaga agctttctcc gccttcacat
      901 gagctaagcc atgagcagca actggtgcca tgatgattgg tgcagtcagc gtttctttgt
      961 caaaatgtgt agttgtatcg ggtaattcta catcttttaa tacatgagga ataatcagct
     1021 gatgattgaa tgcccgttca ttttcttgat aagtaaataa gtcgccagca ccactgctga
     1081 tgtaaccata accaccagct ggaatcactt gtgaagcggc tatttctaaa tcttccatat
     1141 tgataaaatc cactattcct tcatttgtac ctgcttgata cgttttttcc atttcaatgc
     1201 cttccctcta taaaaaattg aatgaataat atgatagcgt ttacacactt tatattactc
     1261 ttccgtattg atacaggcaa ataatctgtt tacatttcag tcattccagt tgttactact
     1321 ctgaaagaaa tgtttagaac acaaatagat aaaaaacgat gggactttga caaaagtcgt
     1381 gtcaaagtcc cttttacgaa atactgtgga tgcctattga aataagtgcg ggttgtttct
     1441 ttgtatttca atcgtttctt gccattcttc gtttaatttt ggattaccgt tttcttcctt
     1501 gaacgcataa ggtcccaatc caaaaagctc agttagtatt tgattcacga ttccatatag
     1561 ctcggtgtga tcggacacat gattattgaa gatcatctca ttatcaccac gtttgaatct
     1621 cacattcggg ctctcatgat gaacctctat gcagttttcc ccttttttta actgacggat
     1681 ttctgaaaac atctcataaa tatcttctcc aataaaacct tcagcaagcg ccagactgtc
     1741 cacaagaccg cctactgtca actcagagat tatcgctttg ttggcaccaa gctttaatgt
     1801 atagcttgtt gcttctacta cgccgcatgc attgcaacca ccttctgata gttcgctttt
     1861 tctgatcaat tgaacttctt tcattttctg ttcctcctct tagttttcta cttatgtatt
     1921 ttttatcacc tgacgtaagt gtattttatc gtcacgatca cgtgatttat tcttcagaga
     1981 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
     2041 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
     2101 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
     2161 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
     2221 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
     2281 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
     2341 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
     2401 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
     2461 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
     2521 acaacgaaat tttcttgaaa ccacaagatg ctgcatttct ttcaatcgac gaaccagaaa
     2581 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
     2641 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
     2701 tcggtaaatt gatggtctac actgctgcgg ctggtatcaa tcctgcacaa gtcttgcctg
     2761 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
     2821 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
     2881 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
     2941 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     3001 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     3061 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     3121 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     3181 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     3241 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     3301 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     3361 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     3421 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     3481 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     3541 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     3601 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     3661 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     3721 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     3781 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     3841 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     3901 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     3961 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     4021 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     4081 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     4141 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     4201 atttatttca ttttatcgga acaaaaatcc ctatcgtcaa aagttattta ggtggtggct
     4261 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     4321 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     4381 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     4441 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     4501 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     4561 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     4621 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     4681 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     4741 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     4801 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     4861 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     4921 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     4981 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     5041 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     5101 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     5161 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     5221 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     5281 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     5341 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     5401 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     5461 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     5521 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     5581 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     5641 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     5701 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     5761 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     5821 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     5881 ctcctttttt catttagaac aatatgtttc agtgattttg acttgcgtta gtctagattc
     5941 tcctaatgtt tgtaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgaggac
     6001 aattacttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     6061 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     6121 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     6181 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     6241 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     6301 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     6361 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     6421 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     6481 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     6541 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     6601 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     6661 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     6721 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     6781 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     6841 tagtgctaaa cgaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc
     6901 aaacaaaaat agctcctcat aattttcgtt tggttgaact tattaccgca gatgcaagct
     6961 tttgaacacc gtttaagaaa gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa
     7021 caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg
     7081 gcaggagaac accgtttaag gaagagactg tgaagtcaac gttttgacct gattattaga
     7141 gaaacaaatg gcaaaatagt tcttcatatt tcgccgtttt tcgagcgaat gccgaggggc
     7201 agctgattga acatcgttta tttggaaaaa gggattgtga caagacgttt gtcacaatcc
     7261 ctttttttat atcttattaa ttcattcttt tgttctattc ttcttcttcc gccatgaatg
     7321 agaaacccat atcaaagaac tataaaggtt atcaaaacag cgtttatgac taaatgaatt
     7381 agagttatct atcactattt ttcaagcgat ttaaaccaaa tggcatacaa tatggcatac
     7441 aaaaacttca ataagccgtg ggggttttta aatttcttct tgttctttaa aatcaatgag
     7501 tggagctaca taatttgaat ccactttttc aaacgtcact ttttgcatcg acgaatgttt
     7561 tctttcccaa gataacgtaa atagatttga ctctggactt ataaagtcta cttcaccttc
     7621 tagtattact ttcttatgca aatattcctc taataaatat agacttgcta aagcatacaa
     7681 cacattttcc aaatttgctt ccttataatt tgaagaacga ccatgtttta ctctgttata
     7741 tattttccac caatctggtg tatcttcagg ccacccatct ccaaaaggtt ttagtgaagt
     7801 caatgttaaa gaattcataa ccctaacacc attatccaaa cacattaatt cgctagattt
     7861 aataacagct ttaaaatcag ttatgctttt attgctactg ctatcaaatc cgcaaagttc
     7921 tttgaaaata atctcacact ccgaaccaat cgctagtagt agtttttgat attcaataga
     7981 gaatgttttg aaattattct catcgagcat cacaaaatct gtggtattaa taaactcatt
     8041 ttctaaatgt agataatact tccaataaaa atttgtaaac tctgctttgt ccattaaatc
     8101 acctcttttg aattataaca aaaaaaacac gtccacaaca gacgtgttct ttgatttcta
     8161 cacaatcagc ttgccaaaac catccaatca gtcctagcat gcctgtatta aaattgaacg
     8221 ttcatcatct atcatctttc ttagtccagc atgtttgcaa ccacacctac aattttcagt
     8281 ccaacaatag atttcgacta gctaagtctt gcaaaattca ggcagctaac ccttcattta
     8341 ctaccttaat tatactaaat acccacaacg tttacaagtg taatcattct aagaaatctg
     8401 tttagctatg atcgttgtat aatctttaaa cgccgttcct tcattgattg aaataatttt
     8461 ataactcttg tttttccaag ctactttcat atcgtttgtt agttcataat cttgatcgta
     8521 tcgaataata aacgtcaacg ttccttcaag aatggtacct acactagcaa gcacgtcttt
     8581 tagcatttgt gaacgcacat tagcccaaca ttcaaataca gtttcttcac tatccaccat
     8641 ctgcccatct tcatctttta cccgttttgt ctttttaaac ttgactctct cagtcaattc
     8701 atttacattc ttaatgattg gcataataag cacctctcat ttgttgtact aatgcttgta
     8761 ccgttactgg tatccgttcg ctgctggctt cttgacggtt caaataccaa tgttgagtaa
     8821 gtaatgacac cgccaaatca aattgctgat aattatccat atctttatcc gttgcgctac
     8881 tatcaatagc actttttata tattcgcctg ctgtatcaat caattgttga ataaagtcat
     8941 catcaatagt gtgatcgatc cgcatacttt tttttatgct atccaaaatt accattttat
     9001 ctctcctaat gaaaaaaagg cagtgggata aaccgccgcc caatattttt ctatactttt
     9061 ggtgttacaa ctggcgtaaa ctcaatatac actgaagcat tttgatcaat tttcttataa
     9121 tcgttgcgaa cgatcactgc aagtccttgt gagtagtaat cgaatttttc ccattgagtc
     9181 gttacttgat ttctacgagc aacaaagata gattgtgcaa tatcccccat aatcataggg
     9241 aatgttccag ctttaggatt ggcaaacaat gtatctgcaa tcaatactac tggcataccg
     9301 aataatgatt taccactagg tgctgtcaca tcgggttgta aaatgtaacg tccatctgaa
     9361 tctttcaatg tatctaggtg gtaatatccg ctttggttta gaattaccat tttatttaat
     9421 gctgggtcta atgccacatt gtatagttgt ttcaaatcgt ccaacgtagc agccgttttc
     9481 ttagtgaatg tttttaataa atccatgata tgcttattgt cagtgttttc taccaatttc
     9541 gctaactgat ctttgacctc ttgtacaata ttcactgctg aatcttccac aacctcattt
     9601 gataaggcaa tcttaccagc acgagtttct actttataat caactgaagt aaacatttca
     9661 gcgtcaatat caccaatttc agcaagttca gcttttgttg ctaacactgc ttgttggtta
     9721 gttgctactg gatatttacc ttgtccattt gatactgttt ttactgttgc atattgagct
     9781 aagttataat ttgaacgttt caaatcaaag acttcaccga ttacttcttc gggaacaact
     9841 gccgctgcat ttacagtagt gactccatca cgcacttcgc cttgtgaacg gatatattct
     9901 tcatacccac gcaattctgt tttttcgtta tcgattaaag ttttttccat gttattactc
     9961 tccttttgtg ctttagaagt ggattctaag ctattgtttg tttgttttgc ttggttgtta
    10021 ctcataaacg attcgtaaga acgcttattt acttggacat ttgatgaatc ataagcggga
    10081 acggtcacga cactaatttc attaagtgct ttgattttat ctattgatcg ttcaattgtg
    10141 ccatcttctt ttttgtcgaa tgaatctaat ccaagcacga acccaaagga catagaatca
    10201 acaacccctt ttgagatatt ctcatacaca tcatttgaat aggtcgtgtc ggttagctct
    10261 gcttcaaaat gtaatcctgt atcatcgaca ttcaatttca acgtacctgc tttgacacta
    10321 gctagtggct ttgaataatc gtgtccatat aataagaaca catttgataa atccacctct
    10381 tttaatgctt cgggtgtaat cacttctaca aagccgccta aatcttttga cggttgcccg
    10441 aatttcaacg catagcctga aatagttttg ccgtctgttg gttgtttttc attttctttc
    10501 tctactttgg gagaatcggc tgttagctca gcttcttctg tcagccgttt ttccttttct
    10561 tcttcattat tcaactggtg taactccttc ctgttctttt aattgatatt tctccaacgt
    10621 atctaaatac gtaaagttca aacttgctag taaacgatcg ccaccatcca tggggggtag
    10681 ccccattttc gaccgcccct cattaatcgt gagaagtgac cctttgacct gatccaatac
    10741 attttttact tttgtttcag gatcagtctc tagtaactga tcggaattaa agcggatatt
    10801 ggtttcaagt ttcgtatcta attcactcac aaacacatta aagtaatgaa tcagcgtact
    10861 ttggatatac tggagattac tttgaacggt gctagaatgt tcattttcaa cgcctaaacg
    10921 ctcaatcggt acaccaaacg ccttggcaat ttgttttgtg ttccaatcat tggaattgat
    10981 aagcttcaaa acatcggtgt ttatttctaa tgttttgtag tccattgttt catcaagaat
    11041 gatcgttcta agagcattat ctccactact tgaaccgttg gcttcttcaa acttttcacg
    11101 aattgcgttc tttgctgatc cgtctaaatc tgacttatga accttcaaaa tacctgaccc
    11161 actgacacca cgagtaaaga aattgtgcaa tgtgcggttg ccagcttgtt gaattttcag
    11221 ttcatcacgt aacgcataaa gtggtggtaa tcctgttaaa ccatcttggg tgaaatattt
    11281 gaagtgcaaa atatcgctag acttcacacg tcttttttta tcaccaatct gataagacaa
    11341 cgtaccatta tctaattgag taaccgttac acttgagtta ggtaataagt gaatttcttc
    11401 taccttttca ccgttgcgtt taatctctgc aaagctatta ccgtttagca acatattgac
    11461 cgccaaagca aactttaaat gccaaccatc catttctgaa ttaggtttct cgtttagtaa
    11521 cttcaccagt tcattatcag cttgtggcat attgtttttg accaattgaa tcggacttga
    11581 agcaatatca ctagcaataa ttcgaacggc tgcaaacaca tcactatttc ttaatgcacg
    11641 aacgcttgta aagctggtgg tgtaatcatc cgattggatt gataccacat gttgtaggaa
    11701 aggatctccc gttgtatttt gtctcgcttt aaaaaatgcc atcttttaac ctcccttctg
    11761 ttctgtattt agcatgaccg ctaccccaat aagcactatg cccagcgcta acaagccata
    11821 gtagacattt gttagtaagg tgatagcaac caacacacac accaatccaa gaagtaaaag
    11881 aacggtatga atgtaattag aaagaaaatt cttcacttgt ataaaattca ttgtctgctt
    11941 tcgctccttc cgttgtggtg aagtaatcca ttgcaaacac ataggcattg attaatgcag
    12001 ctattggatc aattttgtta ctgttctttg atttattaat ctgaatgccg ttgttatccg
    12061 tttttaaaat agcgttgttc actgcatggg tgagaatcgt atttttattg tgaataacgt
    12121 tgccttcata aacttgttct ctaaaagttc gggtcggaac gtttagagta atcgttcctt
    12181 gtcttacttc taacattggg tagttggctt tttcagcttt actgataagc gaattagcgt
    12241 tatacgggtc ataacaaatc cccatacatt ccaaatcatt ttcttgaatc aaatcttgaa
    12301 taaactgaaa tacttggtca tagtccacga ttccactttc taattgagta atggaacact
    12361 caccagcttt ttctagttct cgataaggca aaccgtcgcg cttttctttg tcttgaagcc
    12421 catatttggt ggctacaaag ctatgtgaat cacaataaag ttcgccgtta tctagcggta
    12481 cgatccatga aacgctagtt aaatcatctg ttttagataa atccactccg atataaacgg
    12541 gcttgcctgt aatatcttgt ggttctactt cggttgcctg ccagtcttca gcaatcatat
    12601 agctgtcctc actcgcttgt ctccataaat tgaagttttt caccaataca gaattaaggt
    12661 tattttgttt caaagcaagg ttcacatcat cttgaatggt tggaatcatt acttttttga
    12721 tttcttcact ttcaaaaatc ggattggctt tgatccattt ctcttgatcg tgaatttctt
    12781 cgggatcgtc caattcccat atcgctataa agtacctgtc ggcttgttct tcgcctttta
    12841 gcacacgatc taacaataag tattcctcgt acataggaac gtttaaatca agcccagcgg
    12901 tactaataac acacaataag ccgttctttt gttgggtcat tcctgactta atgacgttgt
    12961 acgttttacg agtttttgct tcgtgccact cgtcaagaat tgcggttgtt ggtgcaaacc
    13021 catctagcgt gctggtttca ctagccagtg ccatagcgaa cgaattagag ggcaagtgct
    13081 gaatcttaga gttcataatt ttcagttgtg gtcttaaaaa cttactagac ttgaccacgt
    13141 ttctaagtga attagacagc atatcatagc ccaatttagc ttgtttcaaa gcgttactta
    13201 caaacaatac ttgccttgct tctgctggtt cttgttccat aatcaacgca ttggcagcca
    13261 tgccacttgc gatatacgtt ttcccattct tacggctcat actggtaaac gctcgattga
    13321 atcgcctgta attcccttct ttagtacgcc agccataaag actgcctaga atgaattttt
    13381 gaaatcctaa catcttgatt gctttaccgt ccgtagatgg tagcaactca atgaatttaa
    13441 tcgctttggt ggcttgttct tcatcgaaca cataagggaa gctatctgac ttagaacgct
    13501 ccaaatcgct tatatgacgc tcacacgctt ggataatttt cttgggtgca atcagcttac
    13561 ctgataggac tttctcaatg taatcattca tgaattcatc aactctttga acgggtcttc
    13621 gggttcatca tttttaacct tgttcaaagc tagttttgat cgtgattcta aggtcatacc
    13681 tagtgaggtg gcttgttttt tcaaatcact catagcttgt gattgaaccc gaaccgctgg
    13741 attggctttc tttactccgt tttcgcatgt gataaacgta cctgattttc ttatctcggc
    13801 ttcggctgtt ttcattcgag caaaggctaa acaataggct accaatgaac cataatctgc
    13861 ttcactcaat ggataatctt tttttaatgt tggtacgata cgattccatt cacttctagc
    13921 gctcgctgcc atccagtcag ggggcgtgat atttattaat ggttgctgct tgaacaattc
    13981 ttctctagcg tccacacgga ttgctttttc ttcacttgat atattgcctt ttgtatcgtg
    14041 taatatttta gggtgtccca tgttgtcctc ctttcagaca aaagaaaaag gaacgtaagc
    14101 aattaagctc acattccttc gtttttcgat cagaattttt ctttatctaa tttatctata
    14161 taaatgattt ttccgttctg catttgaatg gtcacttgac caaaatgagg taactcaatg
    14221 gtgcgctgtt tccctgtttt atcagtaata acaattgtat ttttcaataa atctcctact
    14281 ttcattatat actttttcac ttgtgattac aagtgtaatc gttatttgta attaccctta
    14341 tgccttttca caacggttat ttgtaccaaa atgagtaggc atcgattgtc agtaagtttc
    14401 acactatgcc cccttaactc aaagggtact cttttctcgt ctttgtcttt cattggctgt
    14461 cttggaatta tggtgtgcat ggcataaaga acgaaaatta gtttcatcta accgtctatt
    14521 ccaatcatct tttagttcaa ttttgtgatc tacaacatca gctttcctta tcaagccttc
    14581 ttctaggcaa tcctcacata ctggatgatt taaccgatac aagtatgaag cctttcgcca
    14641 tgttctactt tgatagaatt gaaaatattt accttcgctt tgctttcttt cttcacgctt
    14701 aattgccgtt tgctttgctt tatgcttccc acaatacttc tgcctataat caatcaatgt
    14761 tttacatcca gcatgattac attgtttttt aactgccata gattaccaac caaagcgatc
    14821 tcgttcgtca gaatcatctt gttcccattt tgatttgtat tcaatataag gcgaaactat
    14881 cttcaagcac gttccgtctt taaaattaac tttagtgatt ttatcttcct caaaactaat
    14941 aaagtctaca tcttcttgag tgtttgaata ctcaatgtgt ttctcaccat cgaagtatct
    15001 aatactttcc atatgcataa ttattctacc tccaataaat tagttatcaa ttcatcatct
    15061 gttagtagca aagccaagaa cttatcatgg atgtttaaaa acaaattatc ctcattcgat
    15121 gtaagctctc gttgtctcgc tcgatcatat aggttaagca gacaattaaa ttcatccatt
    15181 agctcacctg tatcatcata gttgccacta tctaccaatg atcccactac tagcgattga
    15241 caccacgcta ctgactcctg cagccaatca ggttcttctt ccattatctt taatacttct
    15301 ggatcaaatg tggaataatc ttctgttacc aatcccacac taaatgccag cgtgttcatt
    15361 agaaaccgat ccatttcatc tgtatattga aagttcggtg ctttgtttgt tgaaataata
    15421 ttgagcattt actttcctcc ttgattcgta acacgtggta acacctacgt aacaggtacc
    15481 cgttaccact caatcccaac ggggacaagg gttctagcgt tttggtaaca ggtaacgggt
    15541 aaaatccagt aacattattc cccagcatat tagctaacta tttcaaaagt tttaaggaaa
    15601 taaggtgtta ccccgttacc aaattgctgt atcccttggg gcagtaaggg taaactagta
    15661 acgggctagg tgttactgac ccgttactac ccgttacttt ttgtactttc cagctcgatt
    15721 tttattttca taggcatttg acttattgta aaatcctcgt cgtttatttc tttttggcag
    15781 ttgcttccgt tcccaaccag gaatactgct aataatcaag gcaatctttt tagtttctgc
    15841 atctgatcga ccattcaata aatcctttgc ttgtttatca aaaacagctt ccaaaatatc
    15901 agcggtgagt acgctatcaa ctaaataaaa ctcttgttgc atagtagctc cattatagaa
    15961 atgctgtaaa tttttctcat tacatttact gtcaggatag ttctgataat aagaacgtct
    16021 cgcccacgct ggagcttctt cccatttata cgggatttcc atttccaaat atttagtgat
    16081 tgcttcttta attggattct ccatcttggc atcctcttga tagtcttttg caatttctaa
    16141 gacttcttta tcttcatcgg gactaaaata gattcgttgt cctttatcaa aaagcacctt
    16201 tgcttcagct aacacttgaa gaaaatattc atcctttgtt ttaaacggat tcatctttgg
    16261 cttattctta caaggcaaag gataaaaacg gcggtttcct gtatcatctt tcaaatactg
    16321 cccatcattc gacgttccaa taaatacaca ttgccttgcc caagctatga cattccgacc
    16381 ataaggaggt ctaattttat cgatcttggc actgatgaag ttttttatct gttcaatttt
    16441 agttttgttc attgaagata attctccaag ttcaataatt acattgcctt gaagctgcat
    16501 ataatcatct ttcttttcac ctagcgaatc taatgaatcg gtgaaaaaat catccgtgta
    16561 aagtaatgct ggaacggtac tcttaccaat cccttgcggt ccatccaaaa ctggaacgat
    16621 ctcaaacttt attcctggtt tatagatacg tgcgactgat ccgactatcc aacgttttgt
    16681 tacttctcgt gtgtaaaggt tgtcgtctac ccctaaaagg tcaataaaca gtgtttccag
    16741 tctaggctgt tgatcccatt taactttttc gatacgttct tttactgggt tgaaaacatt
    16801 ctttctagcc acatttacta ctgctttgtg tattttgttg tcatcaaaaa ctacattgta
    16861 gttttcttca atatatgccg ttaaggcatc ctcaaaactg tcttcccatt ctccagcggt
    16921 acgattgatc caaggagaat cttttaatag gtgaatgtat ccgctaaatt cgttataagc
    16981 aattgttcca gctaaattgc tatcattggt taaaatcaat tctgcattca caacactgtt
    17041 acttttaatc tgatcgtctt tattgcatct taatcgtgac atccaggttt tcaattcaat
    17101 gatattgtct tcacgctcgc tggcttcggg aaagtctaaa atactatcta atttatgctt
    17161 atgagccatg agctttcccc ctcaaatctt ttttaatcat cgatcgaata atttgattca
    17221 cttctttatc ttttagcggt ggttcattgt acgcatttgc tgtataaatc aattctgcta
    17281 aagtttgatt atctacaccg tgccatagta accaaccgca taacttagtt aaatgatcgt
    17341 ttctactacc ttcgccagcg ccttgaacaa tcatatctaa tttttttgct gtccctgttt
    17401 ttgctacggg tcggtattga gtctttattt cttgcggttt ttttagcagt aggttcacta
    17461 gccaatcggg agctggtgca atattattta gagatacgtt ttcaataggt tcatatgctc
    17521 tcccaacact tcttgaaata atcatagttg gcgctattgg aactccttta gtgataatat
    17581 caatcccgct taaactttta cctttatcat cacgcccgaa gatatcagca aatgcacctg
    17641 tttcttgttg gataattaca ccatcaggaa ttttaaagaa gaaatggatt cctttacctg
    17701 gtgtgatttc cgtataagta gaaggaaatg gatcatacat tgaagcaagt tttttataat
    17761 tctcaacgcc gtttcgtgtt tcagaatgtt gatctaggtc aactaccacc atttgatgat
    17821 cttcaagcat taacccaata tttctagttg catcttccgt ccaccattca ttaatttgat
    17881 tagaatcgtt agttgcatca ttccaaccac tagagccttt tattggaatt ttagttccag
    17941 gagataaggg ataaattgac catcccgact tttgtaattc cgttgctgct tgataattat
    18001 ttgccaataa acctctcctc ctttttcaaa atactggtat aaatcattca gacacctcca
    18061 tgagcttcaa ctgctcaagt tcgtatttca acccatcccg aacaaattca gcacctttta
    18121 attgattctg atactttacc catgtattaa atgttttcac ccttgcttct tgcatatcag
    18181 taatgtttaa ccatgcggtt aaaatctgat tttttagctc gttgatttct cgacaaatta
    18241 ttttgtgttc tgcatgtttt ttgagtacat acaaaactgt tagtttttca ttgaatgaat
    18301 ttccaaagta ggttttcccg atattatttt ctagttcagc gatcccttga ttaatttcct
    18361 ctaattcaag gctatcgagt tggtttaata gttttgtttt gtacatctac ttatgccctc
    18421 cagttcttaa ttgatagtcg tactcgtcat aagcagtgaa gagatataac gctaatcctg
    18481 caaagatagc tatttttgca attaatggta ataagccgaa aattccgccg attaaaatgc
    18541 ttcctataat taagtttcgt ttcataatgg ttccctccga ttttttaata tggtataatc
    18601 ggaacaaaga atatcgggtt ttatcccaat taagttgcct ttatcctgtc cgccaagact
    18661 gttatggata aggctttttc tttgttctcg atcatgttaa tgctcctaag ttgtctgcaa
    18721 ttcagcagaa cgattataaa ttttctcact cactgaaaac tgcaacccat atttttcttt
    18781 aacagtcatt aattctactg tctcatctaa aataggttca cgatcaacca acatttcagg
    18841 agtcatatct ttctttttta ccattttgga atgaccataa atagttgata cagctttatt
    18901 ggctatcaca ttcgctttaa tgtagtctac tggttttggt tcagtaatag cgtggcttaa
    18961 tttagtcatt gcttcttttt ggtgttcttt gtctagcatg cggaatactt gaaagccttc
    19021 aagtcctgtg gactggcgta gttctttgat gatgtcgaaa atccagtctt gaaatgcctc
    19081 ggcttctttt ttacggcttt tgaatgccat gcggtaaata ttttttttcg ttgatgatgt
    19141 ttacatcttg tgttctgccc aatgaatcga tgaccttact agtagtaacc ccatctttag
    19201 gcaaagagtg aatggctcta gtaacctgtt taagacctaa cgcttccgca acatctttcg
    19261 ccactgccca ccattcatca ttaatatcga caaatcttat aatgtgtccg ttccaaattt
    19321 cagttttcat tcaacgtcct ccagttcaag ccctagcaag ctcgctaaca actcaacttg
    19381 ctgatacaaa atttcttgat actcttcaat agaaagtcgt tctttaaact ctgcttgtac
    19441 taaatcgttc aagccacgca atactccgct taccgttggt tcttgtggta gcaatttttg
    19501 atagtccatt tgttcgtaca aacattcaaa cgtttcttct agcgctttgc cttccaagtt
    19561 tgctaattct gccgctgtgt cttctgtaag aattgttgat aatacttttt cgattgcttt
    19621 attttgtgtc gattccatta ttatttcctc ctattaatcc ctttgccccc acggcttgcg
    19681 tttatttttg gtattcttcc ataaagcgat cacattcatc ttgtgaaatt cttacgactc
    19741 catcaactat aatcaacttt aaaccgttag gaacatattt ctgcatcata gtattgtatg
    19801 aaacgttcat atacttagca gcttgtgcct tgttcatata ttttggaaac tggtactttt
    19861 tttctaataa tgaaagtaac gttccttgag tttgctctac ttgttgaaca actcgatcgt
    19921 caataatttt aaaagctgga tcatcaagcg tgagtttgtc aatttttaat tccatattta
    19981 tcccccctcg ttagcataca tttctataca aagattttaa aaaaatgcaa gaggctcttg
    20041 aatacatttc tatacctctt ataaactcaa tataccatca taccttttca tttgcaagaa
    20101 ttttaacata cttttttgaa cttttatgtt tcacacatat ttaaatcgtg atatagtaga
    20161 gaaaatatat gaatggtggt gaaaatgtgg ctagaaaaaa ataccccgaa gcagtggaga
    20221 caggacaaag aattagactg gctagaattg aaaagggact ctcaatggaa caacttggag
    20281 gtaaattatc cccaccggcc agtaaaggtg ccgttagtaa ttgggaaaat ggctataatc
    20341 ttcccaataa caccaggctt aaacaacttt caaaggtatt agatgtatcc actacctatc
    20401 tattaaacgg atcttataca ttaagagata tgcatatgat gcccgaaatt aagcgaaatg
    20461 tgcttgaaaa gcaattgaaa aaaactattg aaaaaaataa ctctataatt gattctaatc
    20521 tttctaatgc tatcaataac atcgaagaag ataacctgac tgtctcacag aagatttatt
    20581 taacaaacgc aataaaatat cttaaaacag gatctcgaat tgaatttcct gacgggagta
    20641 ccccaattgc ttatctcgca gctcttctaa atacgctttt acgatatgac tcattaatta
    20701 atgattcaga aaaaaataca gatgagaaaa aagagtatca agaattttat gaaaatgatc
    20761 ttctaaaaga gttcacagct cttgttactg agctaaataa acactattca aaataataat
    20821 aaactgcctc ctccaaccgc ccccacggct tgctacacta tggagggaaa actattatgg
    20881 caacattcaa gcaatatgaa actaaaaaag gtaaattttg gctctatgaa gcctatcttg
    20941 gaatgaacaa aatgactggc aaacctgata aggttcgtag acgaggttac aaaacaaaaa
    21001 aagaagccca gcttgccttg tcacgcttgc aagtggacta cgataaaaac ggtattaaaa
    21061 aagcgaacaa cgaaacgttt aaagaagttt atgacttgtg gctagaaaca tataaaacca
    21121 ctgtccgaga agccaccttc atgaaaactg aaatcaagtt tagaaagtgg attcttccaa
    21181 aatatggaaa tcttcgtgtg aatgaagtca cggtaaaaaa agcccaacaa attgtaaata
    21241 gctgggctga aactactgac caatacaagg tacttcattc aacttctaag cgtattttta
    21301 gctacgctat caatcttggg attattgaga ctaatccact agacaacgta ttaatgccta
    21361 aaagaaccac cactaagcat aaagaaaagg tcaaagttta cagtaaagaa caactgatca
    21421 ccttgtttga ttacctaaac agcaaacctg aaacttatcg aaacgacttt gataaaacgt
    21481 tactacgctt tctcttttat agcggggtta ggatcagtga agcccttgca ttgaattggt
    21541 cagacattga ttttcaagaa aatacggtca ccatcaataa aacactaagc caatccaaaa
    21601 atggctataa aatcagtgat cctaaaactg attgtagtgc tggggttctt ccactagatg
    21661 aaagaacaat ttctatatta aaaaaatggc agattaatca gcggaaatat atgctgacac
    21721 ttggtgtaac tgatccgact atgatttttt gtggtatcta taaagaacta atcacacacc
    21781 acgctattta tgcaagattg attactattt gtgaaaaagc tatggttcca tttctcggtg
    21841 tgcatgtagc aagacacact catgcttcaa tgctcttgga cgctggagct actatggtag
    21901 aagttcaaac tagattacgg catacaaaaa ttagtcagac gatcgattgt tacggtcact
    21961 tggcaaaaga aacgaaagaa aagactgttg aaaaattagt acaacactta aatattgact
    22021 agatggcata caatatggca tacagtaaaa aataaaaaaa ggagaaaccc tatcatatca
    22081 aggtttctcc tagctttata gtctattctt cttcttccgc catgaatgta ttgaatactt
    22141 cttcaatcat gtcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat
    22201 cttcgttttc gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag
    22261 caggatataa aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagtagga
    22321 tttcatataa tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcat
    22381 ggtcgtggga atggttatgt tctgtcataa gattacctct tttcttttat tattaaaata
    22441 attgcttacc tgagccgtct aaataatttt gtaagatcat cacagctgct aatttatcga
    22501 tgactttttt tcgtttggct cttgaagtat ttgcctgctc tactagcata cgttctgcct
    22561 gaacagtggt taatcgttca tcttgataat caactggcaa ggagaatagt tctttgatct
    22621 tttctccgta tgacatggaa gcttctgccc ttggtccgat ggaattgttc atatttttag
    22681 gtaatccaac tacaaaccgg tctacttcat attgtgctac taattccttc aaccgatcaa
    22741 agccaaactc ttcttctgct tcattgatcc gaatgatctc tactccttga gctgtccagc
    22801 caaaggggtc actgatagca acacctactg tcttagaacc gacatccaag cccattactc
    22861 tcataaattc actccatgat ttgagaggta gtccttgaca attacttcca tgatctcatc
    22921 gcgttcgtga cggcgaatca aatttcgagc atcacgataa cgaggaatgt aggacgggtc
    22981 tccagaaagc aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc
    23041 ccgataaaca atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa
    23101 acgtacggtt tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca
    23161 gcttaccttc tacaacctta ttttactcga agaaagggaa aactacaaac aaatattcca
    23221 aacttccgaa taaattaaga aacactattt gtatgttttt ttcctttttt ctgtagcttt
    23281 atcaaaagta ctcttttcat aaagtatacg ctatatttct ttgaatgtat gtttttacac
    23341 aattttttca taatattcat aaaaaattcg aatagctgct actaattttt tgtttttgtt
    23401 gcttgtcctg tctcataatt gatcgtgtag ccatcttgaa cattgtagat aaagacattg
    23461 aattcaattt ttttgtcttc aatgctttct gcttccaatt gcactcccct ggctaccagt
    23521 tcattcccct caaaatgagg agtgacacgg tagcgtacat gatgatttgt ttctttgata
    23581 tatgccgcaa tatcattttc gtgagcaagc atgtatggag cattcaacga acgtgtccct
    23641 gtcatcaagt ttttaatgtt gttattttga ccagtcagct ggaaaccaat taaatggctg
    23701 cgattatata accattgccc gtcacttaat ttcttttgtt tccaacctgt cgggtcaata
    23761 tacaaaggct ctctcttttc agtagggaac aaatcttttc caagcatagc attggcttgt
    23821 cctactctgt tcaatttatc aatatttgaa tagctttccc atgttccttt ggaaaggcta
    23881 agatcctctt ttgtaaattc cggctgattc ccattgattt ctatttcttg atgttttcca
    23941 tcatattctg gcacatccgc tacttgagaa gcttgtggtg tggaatcaaa aatagttgta
    24001 atctgctgaa agatatctga atctttttgt gtggtggaaa caccgaatgc ccctgcaatc
    24061 acagcagcga cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt
    24121 tactaacagc tccctaaatc ttaacatatg ttcccctata aataaatact agaaagaaat
    24181 agaaacagca aaaaataaaa cctgttttaa gatggcttaa aaatacaaaa aagagacgga
    24241 ttcctcgtct ctaatcagtc accactctat ctgtaaggta tttattatta ttcaaaaatt
    24301 ttcttcatac tcaagacaat acactgattt cacaaccgct tggtaaacgg tgcgcaaaag
    24361 cttgcatctg cggtaataag tccatccaag ttaaaaacat aagaagttgt ttttgcttgg
    24421 ctgttcttat tacttgttgc agagcagctt tttcacaacc tattttaagc gggtgacggg
    24481 aatcgaaccc gcgacatcag cttgggaagc tgggatttta ccactaaact acacccgcaa
    24541 tacaaaaaca gtatacgcct cttttattcc ttcctcaagt ttttttattc tttacgcttc
    24601 tggtatgttt tgtagtaaaa taaaactaat caatcaatta ttggagaaga tgctatgacg
    24661 ggaaaagaat tagatctgtt gctggacact tgtttactcg ctgggaaaat catgatggaa
    24721 agcaatgcgg agatgtatcg ggtagaagat acgatgagtc gaattgcttt agcttctgga
    24781 aatttccgtt tggtcagcta tgtcactcaa accggtctct ttattggtct tgatcgtacc
    24841 tcgaccatcc gaatggaaca aattaccaac cgttcgatta atttagaaaa agtagtaaaa
    24901 gtaaatgatc tttcacgtaa atatgttgct tcagagctaa cattagaaga actttactgc
    24961 gaactgcaag caatcgaccg agaccgttct ttctttcctg tctggttgca aatcgttagc
    25021 gcggcagtga tcagtggaac aattatggtg ttatttggcg gaaagatgac cgatttacct
    25081 gtgactttag tcattggagg attgggatac gcagcttatc tatatagttt gaaatttttt
    25141 agaatcaaat ttttatcgga atttttatct tctttattga ttggcatagc cgctatctac
    25201 agcgtacgtt ttggcttagg aagcaatcag gatctaatca ttatcggatg tgtgatgcct
    25261 ttagttcctg gagttcaaat tacgaacgct atccgtgatt tgctagctgg acattatgtc
    25321 tcaggagttt ctcgcggaac agaagcgatg atgactgcta cgatgatcgg ttttgcgatt
    25381 gcttttattt tccagctttt ttattaggaa aggaacacaa gtatggggac gatatttatt
    25441 cagttttctt ttagtttttt agcgactgcg gcatttgccg ttattacaaa tgtacctagg
    25501 cgttcattga tttgttgtgg tctttctggt gcttttggct ggatgatcta ctggatctgt
    25561 gttcaaatag gcggatctgc tgcttttggt tcattgcttg gttctttagg agtagcattc
    25621 atcagcgatc ttttctctaa acgtttgaaa atgcccgtaa cgattttcaa tattcccggg
    25681 atggttcctc tagtccctgg tggtttagct tatcaagcgg ttcgaaatct cgttactgaa
    25741 tcctatcgag aagcggctca ttatgcggtc caagcaatta tgattgctgg agcaatcgct
    25801 ttaggtcttg tattatcaga agtctttaac cataacatcc gtaatttcaa agtaaagcgt
    25861 gacgcaatca tgatgaaacg aaaaaaacgg ctttttaaat aaacgaagtg taacaaagaa
    25921 aaggttgtga aagagacgtt cagccttgag cgatgacaaa atatctcaca aacattgcga
    25981 caagtaaccg caggagcaat aagaagtaat tatgagtatt aaaaaaagtt ttgtaaaaat
    26041 agcttctcac atttttacat aaaattgact taatactgag aaactgactt ttgaacaccc
    26101 tctatctgta aaaaaggggc tgcgacatga tttttgtcac ggcccctctt ctttattcaa
    26161 attttttcca aaacgttcgt tgataataaa cgatccatag agcggaaagt attagaacta
    26221 aaccgatcac aggaatattc actaaccaaa atggataaaa caaagccaac attccgtata
    26281 agacgataag aataatactg atgatcaatg aacccatcat agttgcgact aaacccaaac
    26341 ttcctgaacc tctgttaaat aattggttaa tattggtcca gttcagcagc agcaaccgat
    26401 aatctctagc gaaataacgt agtgacaata agtagcagcc gaataaagaa ccaaatagca
    26461 agctagggat aaaaaacaaa ggcagctgaa aagacagtcc gcttaataat gcgatcccac
    26521 cagttaaaaa actctgcaat atccatccga atctgaactt ttcttttaaa tattggttca
    26581 tagagattgg caaagaacgg ataaacagaa aattttcttg atccaaagaa ataagattgc
    26641 tgataaaaga agtctgatta actgttaaga aagccaatgc gatccctacc aggaaaacaa
    26701 caccgattaa cttattatct aaatcagtta attttatttc gcctcctagg gcaaaagcaa
    26761 gaataaaaat gattggagtt aacaaggaag aagaaagaac ttgcataatc aaattaggtt
    26821 cttttactaa ttgagcattg taactaaata aaagctgatg cacattttgg ttctttttat
    26881 gggttctttt tattttgaac tcactgtttg aatcagtcag ttgctctgta agctttggta
    26941 ctatcagctg ttttacagca aggaaaaatc ccagagccgt gatagctagt attccccaac
    27001 ccaataaacc ttgttgagaa agtggctgac tggcagcatc atagaatggc aataatatcg
    27061 aaatcggatg ccgatctgtt aactcatttt caatagtccc cgtttgatga ttcatccaca
    27121 tgattccagc aaatgcgatc agcatcgagc tgccaagcaa gatactagaa actgtttttc
    27181 tatgtttttt aaacactgct gttcgagcca aaccaaaaac aatcaaacta gccagacaaa
    27241 aaagaataat caaaaataga gaaaacaaaa ggattgagat aatgatagta agcaaaatcc
    27301 ctagatcagc tcgccatccg gttaaaataa acatgactag aagcggaaaa acaaaaggaa
    27361 tgatggtcaa agcaactacc aaaatctttg ctgaaaaaac ctgttcttgt ccaaaaggca
    27421 gaggcaaaaa tacttgcagg tcatttcctt caaaaaagat attataaatg actgatatac
    27481 tttgagttaa acctaacata ccaaaaagtg ccacgtaata agtaaagaaa cctggcaatt
    27541 taacaaagtt catagcaaac atagcaaatc cgtaaatcag taaaaaaatc agccctgaaa
    27601 aaagattttg agacaaaaga tagatagtta atcgctttcc cttttttcct ttctctcttg
    27661 ctttattcgt gagctgtgga ttaacatata aaagattaac tcgaattaat tctaataatt
    27721 gtcgcttata catacggtcc atctcctgtc gcttgccttc cagccatttt caaataaatc
    27781 ttttctagcg tttcatcagg tgagccattc aataattcac taactgcccc ctgataaatc
    27841 aattcacctt tcttcaaaat agccagttta tcgcataact gttgtgcggt atccaataca
    27901 tgtgtagaaa aaatgactgt ttttcctttt gcggcatgag ccttcatcat ttctttcaaa
    27961 tcaaatgctg cttgaggatc aagtccttgc aaaggttcat ccaataccca aatatctgga
    28021 tctggcagca acgtgccgat caaaatcgtt ttttgacgca ttccatgtga aaaacttgcg
    28081 attgtttcat tttgatgact atacatatca aaaagcgctg tcaattctgc taaacgcttt
    28141 tctttttttt gtgtgtctaa ttcataggca gctgaaatca agtcccaata ttctccagcc
    28201 gtcaattgta aaaaaatatc tggggaatca ggcacataac cgattttttg tttgatactc
    28261 aatcgatttt ccgtcaattt cattccatct actaaaatcg ttcccgatga tggttcaata
    28321 atactaacta aacttttaat cgtagttgat tttccggcac cattatgtcc taaaaatcca
    28381 aaaatttccc cttgtcttat cgagagagac aagtctttta atgcttgttt tttcccgtaa
    28441 cttttactta cattgatgaa ttcaatcatt tctgtactcc ttttgtttgt tattactttt
    28501 tattgtaccc ttcgttattg gtggagtaaa tgtcaaaaag agtgcataac aaaaaaggat
    28561 agaaacgact aggctgctac ttgggttgtt ctcttctaca ggaaaacact cttctaacag
    28621 gtaagatatg agtctttgtt ttctttaaaa tgattcattt tctatctttt cttgcataaa
    28681 attctttagt ttatcaatac ttctctttcc catctctagt tgttctccat ttgcaaatgt
    28741 tactatttcg tttactctgc taatagatgt aatgttttca gtattaatga tataagcttt
    28801 taaatcttta cataaatact catattcgag tgatttttta tattttttaa ttgtgccgtt
    28861 tacgagaatc tcttcatctt tcaatactag gcctatttta tttttataac ctggcatgct
    28921 cattagataa aggatttcat cgtaatggat atagtgatcg ctattaccat aacggacagt
    28981 tgtccacctt tttttcgtag catgttgaac aggatagttt tctttttctt tcaataagta
    29041 actttggatg ctttttttat ttttttcata agtgaattct tttatcaaat aaccactagg
    29101 cgtaatcttt cgattaataa cagttattgc tttattcgga tgagacgtta gataaacaat
    29161 tttacacttt tcattctttt ctctaagcat ttcgcctagt tcgataccgc tataataggc
    29221 gttcaaatcg atgtctagga aaaaaatatc tgaggaatag atttcattat ttttaatgaa
    29281 ttcaacaaat tgtataacgt tcaatccctg aacgatctca aatttccaag gacttaataa
    29341 aattctggat tcttccaaaa tttctactag cattttttca aaaaccaaac tatcatccaa
    29401 aataaatata cgcgttacca taaaaagtac ctccactcga tattttacta tcaaaatcat
    29461 ttcatacaac ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt
    29521 ccaacacctt tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca
    29581 aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt
    29641 ctgagctatg tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg
    29701 gagcagagac tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt
    29761 gataatagtc tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta
    29821 ctccttccgg catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa
    29881 tgccgaatcg gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc
    29941 cttcatagct gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa
    30001 cttctaaata gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca
    30061 actcattcat ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta
    30121 agtattgtgt ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc
    30181 ctttggcttc tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta
    30241 ctggcaaagc tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt
    30301 catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct
    30361 gaactaaagc caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga
    30421 cattgatata ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc
    30481 cagataaaat caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata
    30541 acgctaaggg caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc
    30601 atcatcagta gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt
    30661 gctgtgccga acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga
    30721 gacatcgtaa aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc
    30781 gttagcataa ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg
    30841 atcaaataac gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt
    30901 tgatggatca gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga
    30961 tgatggtgat tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga
    31021 aaataggcaa gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag
    31081 cttgccatct gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg
    31141 atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca
    31201 aattgatgaa cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc
    31261 gaaccagtaa aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg
    31321 gtcaaaaata cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct
    31381 tcaataacga tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa
    31441 aacatgagtt tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt
    31501 caaaaaaaac aagttgctct gtaaattttt acacaactct tattttttac caaagcactt
    31561 caattgactt tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat
    31621 aaaagggaga ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca
    31681 acagtcatcc gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt
    31741 tcgtccacga ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc
    31801 atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat
    31861 ttttcatcaa ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg
    31921 ttatctacta gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg
    31981 aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc
    32041 atttgttcaa tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca
    32101 gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat
    32161 acatctgacg gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct
    32221 tccgttaact gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact
    32281 tcgatttgtt ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg
    32341 tctacgatgc gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta
    32401 ttactttgat ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc
    32461 gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct
    32521 acatcataac catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc
    32581 gttgcactat ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca
    32641 gcattattta aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt
    32701 ggaataatat gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt
    32761 ctccatcata gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt
    32821 ttattcttta ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg
    32881 tagactacta attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg
    32941 attccatgat attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat
    33001 ttgaaaacat acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg
    33061 gcgctggaaa atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa
    33121 agatcgctaa aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt
    33181 ccaatgaaac agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag
    33241 agcggatgcg cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata
    33301 gttcttattt aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg
    33361 gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg
    33421 atcagaaaat cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc
    33481 ttttgcaaca ccctgacatt ttgattctag atgaaccaac gaaccattta gatatcgaga
    33541 cactttcctg gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc
    33601 atgaccgtta ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa
    33661 tgcgccatta caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa
    33721 gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg
    33781 ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag
    33841 aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca
    33901 gtatagaaaa gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg
    33961 ataatgtcac cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc
    34021 tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc
    34081 cttttatccg tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag
    34141 gtcaagctga tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta
    34201 ccactccaga aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg
    34261 tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt
    34321 tagcgatgaa caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg
    34381 acaacaaaga agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt
    34441 cccatgaccg atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg
    34501 gcagcaaagt ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag
    34561 aaatcgccga attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat
    34621 ctgattatta ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta
    34681 ctcaggttga agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa
    34741 tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag
    34801 aagctgctag acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat
    34861 tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt
    34921 tttctttttt tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga
    34981 ccaattacta gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg
    35041 tgtatttacc ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc
    35101 ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg
    35161 gtataagttc ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca
    35221 aaccatgatc accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa
    35281 atcccaataa caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt
    35341 gcttaaatca aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat
    35401 cccccatgct tggcctctag cccaacagga attatcacta taaccttgat gcgtacttcc
    35461 atatcttggt tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc
    35521 tcttactaaa taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg
    35581 atttccagtt tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat
    35641 cattctacct tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt
    35701 ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg
    35761 atttcccgtt attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg
    35821 atgattagtg ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc
    35881 tgtttgttga aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca
    35941 aaatccttct gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc
    36001 gcttgggaat tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg
    36061 acaacgctcg atctcattat tccattcttt ttccattaat tctttataac attcatttat
    36121 ccatttttct ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc
    36181 ctttagcata attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc
    36241 ccatcaaaat caattttggg acgattgttc catacttcta aactataatc ccgatcttga
    36301 tgagcaacca ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca
    36361 ggaataaaag tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg
    36421 gattttcgtt taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc
    36481 accctatagt tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca
    36541 atagcaaacc ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga
    36601 atctgccagc cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc
    36661 catgtagagg caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg
    36721 aactgtccat aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct
    36781 cgctgaatcc ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt
    36841 atcgaaaaac caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta
    36901 tggaaatttg gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt
    36961 atgagcaaat tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct
    37021 ttagccgtcc aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc
    37081 cataatggag ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa
    37141 ccaatattca ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc
    37201 cgattgacta accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata
    37261 gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt
    37321 gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta
    37381 tatatctggc taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga
    37441 aatgcaaaag gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt
    37501 tgaagataac aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga
    37561 taaccaaatt tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct
    37621 gcacattcat attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta
    37681 tctaattttt tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt
    37741 tctaccatac gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct
    37801 gttccagctt ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt
    37861 gcgggccccg ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca
    37921 tatcctaccc cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg
    37981 agtggttgat ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt
    38041 actggatgtt tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt
    38101 ctaatcgaag taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag
    38161 tcagttctct atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc
    38221 aatttgcata gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt
    38281 ttgatcgtac agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc
    38341 cagcactggt atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag
    38401 ttttttcgat tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg
    38461 gattttttgt gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc
    38521 tccaacaaga attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt
    38581 ctgcatcaaa ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg
    38641 ttgtatctgc atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag
    38701 cccaatacag taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt
    38761 cattccatgc tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg
    38821 catgtaaccc tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg
    38881 atggtaagaa taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt
    38941 ctaataattg ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa
    39001 agagctgcca taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct
    39061 ttattacgtc tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt
    39121 ctaattccgc atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg
    39181 tctgttcatt catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct
    39241 agaacgaact agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca
    39301 ttctccttaa atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt
    39361 gctaaaatta gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata
    39421 tttttgataa ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat
    39481 agatattgat tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat
    39541 aaaatcggta cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca
    39601 gcctccatca gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt
    39661 gtactggcaa tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc
    39721 ataatgtaaa ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact
    39781 gtattgaaca acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt
    39841 gtagaactgg ctgtagctaa aactgtactg attacagcaa taacaatcga attccaaata
    39901 atcaagggat aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca
    39961 aacttgaaat tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc
    40021 atatataaat atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa
    40081 attctgcttt gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc
    40141 cgataatata acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc
    40201 aaccatttca aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg
    40261 tccattggaa gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt
    40321 ggctaaaact gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa
    40381 ttgccgaaat ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc
    40441 ttgaagacca gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat
    40501 gatcattgat attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag
    40561 cgcattcaaa aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc
    40621 tacagccacc gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc
    40681 ttttaactgt gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg
    40741 aattgatcct gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg
    40801 gaagatagct agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa
    40861 actaaaagca aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa
    40921 aggtgtgacc aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct
    40981 gttctttatt gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt
    41041 tttaatcacc agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat
    41101 atgaattttc aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg
    41161 tagcatctga ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca
    41221 atagttcata gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt
    41281 gataggccaa tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt
    41341 catctgtcag cataaatgta agaaattgtt gtgccacttg tggatttttt gcatctttag
    41401 ggacataaaa acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag
    41461 gaatcagtgg agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg
    41521 tagcatacca gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt
    41581 gtgcaccgta gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat
    41641 gtttcgcttc tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct
    41701 gctgggctag catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa
    41761 aactaccagt tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga
    41821 tttcttcaaa atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg
    41881 gggtaaaatc actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat
    41941 tcaatttttg atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac
    42001 tataaaaagc atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga
    42061 taatatccgg catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg
    42121 ttcccgaagc tacattcaca atttttaagt caatatttgc tccagttttg cggttataga
    42181 tttttgcagc attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg
    42241 tagtggcttt ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc
    42301 cacatgcact taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc
    42361 tttttttcat gtctatccct tctttctttt caagttctta tataaaacgc tttcacttat
    42421 gtatattaac ggaattagta acacaaatat ttatataaaa aaatatttta cttgtcgttt
    42481 tggcttttca tttttgttat aatagtcatt aattataaat agaaagcttt ttatataaaa
    42541 agagaaagga aagtggttct tttgttaaca aacaaccaat cctccaatct tgtcaaaaaa
    42601 acactaacct attccataga tacccatctt catttatctg ctgctcccta ttttcgacta
    42661 ctaataaatg aaagcgctaa tttatatgct gaatttgcac ataaatctat agaactagat
    42721 aatccttccc ttttattcat tgataaacag caagaagtcc aaatcttttc tactcaaaag
    42781 gaaacacaaa cttttatcat tatccagttt tattttgatg agcaattatt agcacatatt
    42841 acgggacaat ctttttctat agatcaagaa ataacatcta tcattacttt tctgaaaaat
    42901 gaacagttca aaattgatta tctaaaacat agtaagccag ctatttccta tgatcaggaa
    42961 atcaattctt ttttcatact atgccaacag atgagcattc atttgctttc cttattagtt
    43021 acttattgtc tgcaaaaaat tcaattgtct tccttaccaa ggcattatca acaggaactt
    43081 gctttattga caataccttc aaatgagaaa gtcattccta aaaaaaagca tattctttat
    43141 tcagatgaat tagttgatcc aatcatacga tatttagaag aacatctttc agaaaatttt
    43201 ttgcttgaac aattagccca aatttttttt atcagtccag ccagcttaaa aaaacatttt
    43261 agaaaagtga atggaaaaag tgttattagc tattttaaag aactaaaaat ggaaaaagca
    43321 aaacgtctcg tgctagaaaa tgaattaacc tatacagaaa ttgcagatat attaggattt
    43381 cattcgatcc atcatttttc ttcagctttt aaaaaatata cagggttatc cccttcaaag
    43441 tattatctat ccttaaaata gaaaaaaatt tttaggtttt atccacaaat tatttctcta
    43501 tattttctgt ctattcaaaa taaacaataa ttttccggca caattatttg ctgtttttta
    43561 ccataacata gtacgatcaa cgtagaaaag gagatgtttt tatgaaagtt gttgtcattg
    43621 gcggtgtagc tggcggtcct tcatttgcca ctcgtttccg tcgattgaat gaagcgcacg
    43681 aaatcattat ctatgaacgc ggagaaaata tttcttatgc aagttgtgct ttgccttatt
    43741 atttaggagg tgtgatcact gaccgtgact cgctgatcga acgtacacca gaaatattga
    43801 aaacaaaaaa caacatcgat gtctttacta aacacgaagt aacagcaatc gatccttcta
    43861 ctaagcgatt aacagttaaa gacctatcca caaatgaaga aataaaaaca gattacgata
    43921 agttgatcat ctcttctggt gctagaccag attatccgga tattcccgga gtttttgaag
    43981 cagaaaacgg ctttgtcctc cgtagtgtga cggatgcgga tcgaatcaaa tcgttccttg
    44041 aagaaaaaaa tccacaacat gtcgtcattc ttggtgcagg tgttatgggt ctggaattag
    44101 ctgagaattt caagcatcgc ggcttagacg tgactttaat cgatcaattg ccacaagtcg
    44161 ctttccctta tgatccagaa attgctaatt tagtttatga caaattgctg aaagaaggat
    44221 tagccgttca tttagaaaca agagttactg agatccgtga taaaggtcga gaaatagtat
    44281 tatcagatgg ctccgtcctt tctgctgata tgctaatttt tgctgttggt gtttctccga
    44341 agaatgaagt ggtgaaagca accggcatac aattatcaga tacaggtcag atcattgttg
    44401 atgaccagtt acaaaccaat ctgccggaca tctatgcgat tggcgatatt atcgaaacga
    44461 ctagtgtagt gactggtcag ccgatccaaa gtatgctttc cagtgcggcc aatcgtcaag
    44521 gacatatgct ggcagatatt ttaaatggta cgcctatgcg ctatcgcgga tatattggtg
    44581 caggtgtcgc aaaaatcttt gatcatacag caagttatgc gggaatgaca gaacatgaat
    44641 taaaagcatc aggcataaca aattataaaa ctgtttttat cactcccttt gaccatgcct
    44701 atttctatcc agaagctaca agattaaatc taaagctgat ttttgatgca gatagcggtc
    44761 gtattttagg tggacaagca tttggagaaa aaggtgtcga taaacggatg ggagaacttt
    44821 ctgtagcaat caccggaaac ttgacagtct ttgatttgcc tgatttggag ttgccttact
    44881 ctccaccgta ttctactacc cgtgacccgt tgaatatagc tggttatgtc gcaatcaatc
    44941 aaatgacgaa tatcgtagaa acgatcaaag caagtgatat acccgaaaac gatttgaaag
    45001 aagcgttctt tttagatata cgtgaaccta ataaagcacc ttctggcagt atctcggcaa
    45061 caaaaaatat tccaatgaat gagttacgag atcgaatcaa tgaaatccca aaagataaaa
    45121 aagtttacat tactttcaga agaggattga atacttatac ttctgcccga atcttggcag
    45181 gtttgggtat caaagcgatt ttgattgaag aataaaaaaa cgaccgcggt gggtagccgc
    45241 ggtctaggag ttatttttac ttgaggagta aaaatgaaaa agtatatagg gttgttggtt
    45301 ggtatgtata tagaatagaa gataaatgtg aagaaattgt gcattatttc ttcacattta
    45361 ttaggttata gtttaagaaa gtttaaacat tgcgcaattt aattatttta gctgtttctt
    45421 ttcataaaaa aacatgttgt gactctgaga caaatcatag tccatagtga tgtctctaga
    45481 gtaactacgc ctaatattac gaggcatcat attaatctat cagcactagt acctaaacta
    45541 gcatcccttt cgaatatttt ttcttatata ataaagtttg ctttcactcc tctaatctca
    45601 ccaaatcgac tggcttgcgt tgtgtgatac taggaactga ttggtttaga acgacttttc
    45661 tattgtaatc atatttttcg ccatcctcgt tgtcttctaa tccttcaatg aatgatccct
    45721 aatgtgtgta ttaaataatt taattttttt gttcctattt atttaac
//
LOCUS       DACCXW010000007        43747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCXW010000007 DACCXW010000000
VERSION     DACCXW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.007"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(107..451)
                     /locus_tag="IVY71_000313"
     CDS             complement(107..451)
                     /locus_tag="IVY71_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7984389.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(444..878)
                     /locus_tag="IVY71_000314"
     CDS             complement(444..878)
                     /locus_tag="IVY71_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7984390.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1016..1753
                     /locus_tag="IVY71_000315"
     CDS             1016..1753
                     /locus_tag="IVY71_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984391.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1746..2957
                     /locus_tag="IVY71_000316"
     CDS             1746..2957
                     /locus_tag="IVY71_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984392.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3164..3943
                     /locus_tag="IVY71_000317"
     CDS             3164..3943
                     /locus_tag="IVY71_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7984393.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4013..4675
                     /gene="trmB"
                     /locus_tag="IVY71_000318"
     CDS             4013..4675
                     /gene="trmB"
                     /locus_tag="IVY71_000318"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7984394.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4773..6527)
                     /gene="ade"
                     /locus_tag="IVY71_000319"
     CDS             complement(4773..6527)
                     /gene="ade"
                     /locus_tag="IVY71_000319"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7984395.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6659..7588
                     /gene="cpaF"
                     /locus_tag="IVY71_000320"
     CDS             6659..7588
                     /gene="cpaF"
                     /locus_tag="IVY71_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7984396.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7545..8594
                     /locus_tag="IVY71_000321"
     CDS             7545..8594
                     /locus_tag="IVY71_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP7984397.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8591..8869
                     /locus_tag="IVY71_000322"
     CDS             8591..8869
                     /locus_tag="IVY71_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7984398.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8844..9314
                     /locus_tag="IVY71_000323"
     CDS             8844..9314
                     /locus_tag="IVY71_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7984399.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9331..9633
                     /locus_tag="IVY71_000324"
     CDS             9331..9633
                     /locus_tag="IVY71_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984400.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9650..10021
                     /locus_tag="IVY71_000325"
     CDS             9650..10021
                     /locus_tag="IVY71_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7984401.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            9993..10352
                     /locus_tag="IVY71_000326"
     CDS             9993..10352
                     /locus_tag="IVY71_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984402.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10524..11531
                     /locus_tag="IVY71_000327"
     CDS             10524..11531
                     /locus_tag="IVY71_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7984403.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11556..12740
                     /locus_tag="IVY71_000328"
     CDS             11556..12740
                     /locus_tag="IVY71_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7984404.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13177..14760)
                     /locus_tag="IVY71_000329"
     CDS             complement(13177..14760)
                     /locus_tag="IVY71_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7984405.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15338..15481)
                     /locus_tag="IVY71_000330"
     CDS             complement(15338..15481)
                     /locus_tag="IVY71_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7984406.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15704..16183)
                     /locus_tag="IVY71_000331"
     CDS             complement(15704..16183)
                     /locus_tag="IVY71_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7984407.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16256..16540)
                     /locus_tag="IVY71_000332"
     CDS             complement(16256..16540)
                     /locus_tag="IVY71_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984408.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16618..16812)
                     /gene="ssrS"
                     /locus_tag="IVY71_000333"
     ncRNA           complement(16618..16812)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVY71_000333"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16884..18170)
                     /locus_tag="IVY71_000334"
     CDS             complement(16884..18170)
                     /locus_tag="IVY71_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7984409.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18308..18979
                     /locus_tag="IVY71_000335"
     CDS             18308..18979
                     /locus_tag="IVY71_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7984410.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18945..19433
                     /locus_tag="IVY71_000336"
     CDS             18945..19433
                     /locus_tag="IVY71_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7984411.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19446..20393
                     /gene="prmA"
                     /locus_tag="IVY71_000337"
     CDS             19446..20393
                     /gene="prmA"
                     /locus_tag="IVY71_000337"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7984412.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20394..21158
                     /locus_tag="IVY71_000338"
     CDS             20394..21158
                     /locus_tag="IVY71_000338"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7984413.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21391..23604
                     /locus_tag="IVY71_000339"
     CDS             21391..23604
                     /locus_tag="IVY71_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7984414.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23615..24061
                     /locus_tag="IVY71_000340"
     CDS             23615..24061
                     /locus_tag="IVY71_000340"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7984415.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24232..25491
                     /locus_tag="IVY71_000341"
     CDS             24232..25491
                     /locus_tag="IVY71_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7984416.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25580..26527)
                     /locus_tag="IVY71_000342"
     CDS             complement(25580..26527)
                     /locus_tag="IVY71_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7984417.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26921..28213
                     /locus_tag="IVY71_000343"
     CDS             26921..28213
                     /locus_tag="IVY71_000343"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7984418.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28228..29997
                     /gene="aspS"
                     /locus_tag="IVY71_000344"
     CDS             28228..29997
                     /gene="aspS"
                     /locus_tag="IVY71_000344"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7984419.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30106..30981
                     /locus_tag="IVY71_000345"
     CDS             30106..30981
                     /locus_tag="IVY71_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7984420.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31247..33130
                     /locus_tag="IVY71_000346"
     CDS             31247..33130
                     /locus_tag="IVY71_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7984421.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33205..33489)
                     /locus_tag="IVY71_000347"
     CDS             complement(33205..33489)
                     /locus_tag="IVY71_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7984422.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33479..34213)
                     /locus_tag="IVY71_000348"
     CDS             complement(33479..34213)
                     /locus_tag="IVY71_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7984423.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34342..34965
                     /locus_tag="IVY71_000349"
     CDS             34342..34965
                     /locus_tag="IVY71_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7984424.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35157..36278
                     /locus_tag="IVY71_000350"
     CDS             35157..36278
                     /locus_tag="IVY71_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7984425.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36285..39413
                     /locus_tag="IVY71_000351"
     CDS             36285..39413
                     /locus_tag="IVY71_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7984426.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39432..42626
                     /locus_tag="IVY71_000352"
     CDS             39432..42626
                     /locus_tag="IVY71_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7984427.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42772..43479)
                     /locus_tag="IVY71_000353"
     CDS             complement(42772..43479)
                     /locus_tag="IVY71_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7984428.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14111 a         7769 c         9236 g        12631 t
ORIGIN      
        1 gttgtcgtgg tagtttcaaa caaaattcaa aatttcatca cattcctagg aaatacgcaa
       61 acttttctca cttatatagt taaatctgtt gagaaaataa tcattattat atttgttcag
      121 gatactctgg cagatcagct tttagttttt ctacttgttc attgatttgt gtgatacgtg
      181 gttcggcttg gaatttaaaa tcttcgatat cttttttgat tgcttcttgg aaagcaggaa
      241 tcacagattc agctgtttct tttgtttcga tcaatgcgct tttaaaatga ttcaggctgt
      301 tatttaaatc atttgtcaga gttgttgctt cttccaattc ttcgatcagt ttctttcttg
      361 tttcattccc gctcctgggc gccaataaca gacctccgac agtgccagca gcagctccaa
      421 ataacagtcc tttgaaaaag tttttaatca tcgcaggaca cctgctttac gattgtttct
      481 tgaatagctt ttagttcttc cgatccataa ctatcttgat gattgacaaa gtgaatagaa
      541 aaatcatcct ctttgctgta gcgagggact aaatggatat gtgaatgaaa aacagattga
      601 taggctagtt cacggttgtt attcaagata ttaagacctt tcatttctgg aaaagctttt
      661 tccaatgctt gagcaatctt aggcacacgc gaaaaaacct ctcctgcaag ttcgggatca
      721 tattcaaaaa tatctgctac atgacgttta ggcacaacta atgtgtgtcc tttcgttgct
      781 tgagagatgt ctaaaaaagc atagaccttg tcgtcttcat atattttata gcttgggatt
      841 tcttgattga tgattttgca aaaaatacag ttttccatat aaaacatcct ttctataaag
      901 aataaatact tcagcttaac tttaccataa gttaaagcta ttgtaaggaa aaaaacaaaa
      961 taccgtcatt ttcttcgtgg tataataaat aaaaataaat ggcggaggaa aaagaatgag
     1021 tttaattatc gatcatgtca caggggggta cggacatctc cctgtgttaa aagatatcag
     1081 tttcgatgta cagcctggag agatagtagg gttgattgga ctgaacggag cagggaaaag
     1141 tacgaccatc aagcacatta tcggtctgct ggaagcttca aaaggaacga tagaaatcaa
     1201 tcatctatcc ttaaagaaaa atacggaaga atatcgcaaa caaattgggt atattccaga
     1261 gactccttct ttatatgaag aattgacatt gagagaacat atcgaagtca ctgcgatggc
     1321 ctacgatata cctatggata tagcaatgaa aagagcagaa aagttgttgg agacttttcg
     1381 gttatctaat cgtcttgact ggttcccagc taatttttca aagggaatga aacagaaagt
     1441 gatgatctta tgcgcctttt tgatcgagcc ttctttatat atcatcgacg aaccattttt
     1501 aggcttggat ccactagcta tcaatgcatt gcttgaattg atggtagaaa tgaaaaaaga
     1561 aggagccggt attttgatgt ctacgcatat tttagctact gcagaaaaat attgtgatcg
     1621 ttttgtggtc ttacataacg gagagatacg agcagacggt tctcttgaga tgttgagaaa
     1681 agaatttcag ctgccggatt cttctttgga cgaaatctat attgctttaa caaaggagca
     1741 ggcacatgag tagttttttt caaaaacgtt tagcaagaca tcaaaagaaa atgatgaaat
     1801 atatgcgtta tgtattgaac gatcattttg ttttagtttg tcttttcttg attggcggtg
     1861 tgggattata ttattctaat tggttgaaaa ccctgcagcc accgtttgca attggagggc
     1921 tcataatctc tgtattttgg atgctctgtt tattcgtggg taagatggct acttttgcag
     1981 aggcagcaga tattgttttt ttacttccta aagaaaaaga aatgcaaacc tatttggaac
     2041 gagcttttcg ctattcttgc atttttccgt tgatcttttt gatattggca tgcggattgg
     2101 ccatgccatt agtggtggtt agtacggagc aatccttttc gatgtttttc gtctatctga
     2161 tcatattatg gagtttgaaa ttcagtcatc tgcaaatcaa gagagcagaa ttgtttcgac
     2221 tagatcaaca agttattcgt tattggaaat taggctggtt tttttcttct ctgatctttt
     2281 tgtttctatc attttattgg ctaatatggg caggaatagc aggtgcatta gtacaggcaa
     2341 gtctatatta ctggtttttg tggaagcaac aagaaaaagg attggattgg gagaaaatgg
     2401 tgcatgtaga agaacatagg ttgcatcaga tctatcaatt tatcaattta ttcacagatg
     2461 ttcctgaaat tacggcaaag atcaaaagaa gacgctattt ggatcctata ttgactcata
     2521 ttaaaagaga atcaaaaaac acttatcttt acttgtttgc aagaagattg gcaagaggtt
     2581 ctgattttag tggtctttat ttccgattag taattatagg tgggttattg attgccggag
     2641 tagtcgactt ttattttagt ctgattatcg gtttcttatt tttatatttg atcggtttcc
     2701 aactgttacc tttagcgaac cagttccgct atatggttct cgttcagctt tatccaatcc
     2761 ataagaatca gaagaagcag gcgattcagt atctactcac atgggtatta attatcgcgt
     2821 cggttatttt tggtttgatt gctgcagtcg tattagatgg gcagcagcga tgggttccgc
     2881 tagcggctta tttgattgtt actatgctct ttgttaaact atatgtacca actcgtttga
     2941 agaagatgtc agactgaaaa caaagctgtg tagggtaaaa atgagaatga aaaatagagc
     3001 gttgacagta agaatgctgt tcgttctatt ttttttgaga aaagctggtt ttttgaatgg
     3061 caattaaatg aaaaagaacg tatatgtgct tgacgtgtag tgttcaggcg actagaataa
     3121 taagtactgc aaaacaaaga gtaagaggac gggagaatgc atgatggagt ttcagttaga
     3181 ccaagagtgg cggttacgtc ccatcaaagg cgacacagga aaaacataca taggcatgcg
     3241 tgatgaggac aaagtcttta ttaaacgaaa cacgactccg atgttggctg ctctttcaag
     3301 ggaaggaatt acaccaaaac ttgtttggac aaaacgaacg gggaatggcg atactttgac
     3361 agcacaagag tggctggaag gtcgcttgct gactcctaat gagattggtc aaagaaacga
     3421 cgtgattgat gttttgtacc atttacatca ttcaaattct cttaaaacca tgctgacccg
     3481 tatcggtggt cgtgtcatga cgccggagaa gatgctgcaa aaatatgaaa aagaattacc
     3541 ggaagaactc cagaaaaatc aatttctgca aattgtccat cgttttcttc gtgagcagat
     3601 cccgtcaata gataaggaaa acttatcagt agtacacgga gatgtaaacc atcgtaattg
     3661 gttagtatgt aaaaattatt tatatctagt cgattgggat tcaatcatgt ttgcagaccc
     3721 tgcggtggat attgcaacga ttttaggaaa ttatgtgcca ctgtctagct ggaatcagtg
     3781 gctgatcagt tatggcatac gcccgactga cgaaatgctt gaaaaatttc attggtatgc
     3841 ggccatgaat ttgctccaag aaatcaccag atattgcaaa cgtgaagatc atcgacggat
     3901 gaacgaagaa attttacaat taaaacgaat tttcagtggc tgaggtttgg cccaaactct
     3961 gcggagattt ggccagacct ttttaattag aggaaaggaa tggagataaa taatgcgtgt
     4021 ccgaaatcgt ccgggagcag cggaaatgtt agcggctcac ccgaattttg taattagtga
     4081 tccaacattg tggaagggaa aatggaatga attgtttgaa aacgatcatc caatccacat
     4141 tgagattggt atggggaaag gccagttcat cactggaatg gcaaaagccc atcctgaaat
     4201 caattatatt ggagtagaaa tgcaggtcag tgttgtttcg attgcactag acaagttgat
     4261 cgaacaaccg cttcctaacc tgaaacttct acatgtggat ggatcagcgt tgacagagta
     4321 tttcgctgac agcgaagtag atcaaatcta tctgaatttt tccgatccat ggccaaaaaa
     4381 acgtcatgaa aaacggcgat tgacatataa gacttttctt gctgtagatg aacagattct
     4441 tcgtccaaat ggagaaatcc atttcaaaac agataatcaa ggattgtttg agtattctct
     4501 ggcaagtttt tctcaatatg gcatgatcct caaacaggta tggttagatt tacatcaaag
     4561 tcaatttgaa ggcaatatta tgacggagta tgaagaaaaa ttttcttcaa aaggtcaacg
     4621 tatttatcga gtagaagctc aatttcaaga taagaccaaa aagaaaagtc tgtgaaacta
     4681 actttctgct ttcataaaaa cattaatatc cataaataaa tcccagaaaa tgaacattac
     4741 agatgtaatg tattttctgg gatttatttg atttaaaatg tatttatttc atcggttttt
     4801 ccacaaggtg gaatcaattg gaagcgagaa aatgaaaata atcctttgtc ggtgatcttc
     4861 aattcgggta tgacagataa tgttaagaaa gaaagtgtca aaaaaggatc aaaggtttta
     4921 gctgctccta gttgatgtgc tttagttatt aattcattca actgttcata gacgatcgaa
     4981 aaaggttcct gtgtcatgat tccgcctata ggaagagaaa cacaagctat ttcctgctga
     5041 ttgttgacaa cggtcatccc gccacctttt tgaatcaaaa gattagcagc atacagcata
     5101 tcctcgtcgt ttgtcccaac gacaactaga ttatgcgagt catgggcaat cgttgtcgct
     5161 atcgctcctt gtttcagctg aaatcctttc actatgccta atccaatgtt tcccgtttga
     5221 tggtgacgct cgatcaccgc aatctttaat tggtcttttt cgattgaagg aatgaatcgt
     5281 tcgccttgtc ggtccacttt ttctaccaaa tctttagtca ctagactatt cgggataatt
     5341 tcgatcacat gggcttgtga agaagaaaga agcagctcta acgaatgtgg atttaatggt
     5401 tgagcgttta gttttggcag acaacacgta tatgaatttg aatgatcagc aaataaatgg
     5461 tcttggagtt gcccagattg aacaactaat gttcccttat agaaaacttc atgaattggc
     5521 agcttggtaa gatcgtctgt taagaagaaa tcagcttgat acccaggggc aatcgcacct
     5581 agtgttttca aatcaaaaca ttctgctgca ttcaatgttg ccatctgaat ggcttgtaat
     5641 ggatcgattc caagtgcaat agcttgacgg acgatatgat cgattgatcc ttcttttact
     5701 aaatcattga tcagtttatc atcggtgaca aagaagcatc ttcttgaatt ttcaggtgtg
     5761 atcgccggta gtagcgatgc cagatctttt gcaacggtcc cttctcgaac cattaaatac
     5821 ataccaagat ccaaacgttc tttagcttct tttgcagttg tagcttcatg atccgtccga
     5881 atacctgctg caggataaac atttaactct tccatgccga taccagctgc atgcccgtca
     5941 atttttttct tatgctggtg catcaattgg atcttttcta ggatgtctgt ttcattattt
     6001 gctactgctt gatagttcat gacttcagct aagcctaaaa cttcgggttt ttgtaaaaaa
     6061 ggggctaatt tttttgcatt taaatttgca ccattggtct caaaaggggt tacaggtaca
     6121 caagaaggta gcataaaaaa gatattcatg agtgtttgtc ttgcatcttc tatcataaac
     6181 tcaagacctg tactcccagc aatgtttcct atttcatggg gatctgtcac tacagtagta
     6241 actccatgaa gcaaggatac ttttgaaaat tcgcttggtg ataaaagtga gctttcgata
     6301 tgcacatggc catcgatgaa tcctggcact agatattgcc cattagcatt atgtacgctt
     6361 tttccttcgt attcaccgat tcctacgatc attccttcgc agattgctac atctttttcc
     6421 atgatttctt tcgtaaagac gttgacgatc ttagcatgct tgatcactag gtcagcaggc
     6481 ttttttttac ttgctacttc gatatatttc cttaatgttg tttccaatgg agtgcctccc
     6541 aagataaatt tttataagta taacataaat tcggtttttg ccaatatttt tttgtaaatt
     6601 cattttattt gaaacaaaaa gatcaaaaag acgtatgttt tttgtggagg ggagaataat
     6661 ggaaataaaa cgattatcgg atcaaatgat aagtgaaagt tttgccaaac atgcccaaga
     6721 cgtatatata ttgccagctt cagaagtatt ttacatctat tttcgctatg gaaaaaaacg
     6781 aacattctgg aagaaagtct cttcggcaga tggacagcaa ctgatctcaa gatttaagta
     6841 tttgggacag atggatatcg gagaaaaaag aaaagctcag ttaggtgcat ttacttattc
     6901 tttagaaaaa caggaagttc gtctgcggtt gtcttctgta ggagattacc ttcaacgaga
     6961 gagtttagtg attcggctat tatacggtac ttttgaaaca ccccatcgtt gctttagttc
     7021 tcgagatttt gcaactattt tacagaaaac cgtcagacgt ggattatacc ttttcagcgg
     7081 accagtcgga tcaggcaaga cttctctaat gtatcggttg gctttggaag aaaaattgca
     7141 agtgattact atcgaagatc ctgtagagat agaagaaccg ttttttctgc agctacagat
     7201 caatccgaaa atagcacaga cttatgatac acttttgaga ctcgcattac ggcatcgtcc
     7261 agacttgcta atcattggcg agataagaga tgaatcaact gcacatgctg cggtaagagc
     7321 agcactaaca gggcaccggg tttttgcaac cgtacatgca cgcaatcttg aaggtacatt
     7381 ggtcagaatc catgaactga caaagaaaaa agaagagcta tctgaatgtt tagctggtgt
     7441 catttatcaa gaacttttgg aagatgccaa tcaccatcct tctgttttat ggagttatca
     7501 atttatggag gaagaatcag taaaaaaatc atgggaggaa agttatgaag aagctcagga
     7561 aaatatttgg cagtcagaac tattctaaag gattaacaag aagccagcaa aaccagtttg
     7621 cacaaaccat gggagaactg cttttatctg gttttagtct gcaagaatcc ctttatttat
     7681 tactcacgat ccgttcgttt ccacctgtta ttttggcaaa tatccaacat cagctaaagg
     7741 aagggcaagc gtttagtgag gtactttcgc aaataggata tggagcagag caactggtac
     7801 agatcgagtt agctgaacgt catgggaatc tgccgacaac tttgttggaa attgccaaac
     7861 agtttcggct tgttgaagga tttcgcaatg atttgaaaaa gctgcttgca tatccgatgc
     7921 tattgctcac ctttttgcta ggtatcttgt ttagtatgcg gttgttcgtt ttgcctcagc
     7981 ttattggttc aggaatggtc atgcaagagc attggggtat tcagctgata caagtctttc
     8041 attggtatgt tctactattc atcctagcgg ttactctatt ggctttacta gtaaggtatc
     8101 gtctgatgaa gttgtctcct gttgatcaga gtgaatggat aagcagacaa ttattgattg
     8161 gaccgatata ttccttgtat caatcggctt atttgtcact agagattgga aagctttttc
     8221 atgaaggttt cgaattaagg caaatcatag tctgcttaaa ggaaacgaaa caagaaagtc
     8281 tgattcagca tcttgcctct aagttgattc aaggattgga ggcaggtatg tcattggctg
     8341 agcagttcca gaattatcgt tttctatcag aagaattcag taaaattgtt ttacagggag
     8401 aggcaaaggg aagtttaggt aaagaactga ttttttacag cgaatggaca agaaaacagc
     8461 ttttcaaacg tcttcatcac tggatgcatt ttattcagcc aatcattttt ttgggcgtag
     8521 cggctttgat tttattagtt tatgccgctg ttttattacc tgtttacgga agcatagagg
     8581 aggttttgcc atgaaaaaga gaaaaatatc ttattatagc ggttttacat tgatcgaaat
     8641 gctaatcgtc ttactgatca tcagtgtatt agtgttgttg ttcgtcccta atttgtcgcg
     8701 ttataggaat catgtagacc aagaaagtcg tgaagcgatc atccagttgg tagacacaca
     8761 aaaagagctc tatgcgcttc aaaataatgg aagagttcct actgtggagg aattgttgaa
     8821 tgaaggctat atcaaacgag aacatgcaga aatctatcag cggccttgaa ggtaaggttt
     8881 tagcatcagg atatacgttg tttgaatcac tcttactgat tttattactt acaacgatcc
     8941 tttcttttcc agttctcgca ttttctgcat ggaagaatga attggcagtc tatcaatttt
     9001 tcggccaatt tgaaaaacga atctattcta cgcaaaaaat cgctattgtg aatcagatcc
     9061 aaacaggctt ttattggaat agtgaagaga atcaaatcat ttttcatgtt ccgaatccag
     9121 ataaaataaa ttgggaagta ttagatattc ctgatgagat taccttgaaa cgccatgctt
     9181 ctattacttt tgctgctaga accgggaatg aaagttcact aaaagcttat caattttatt
     9241 gggaagaaaa agagcggacg attacgtatc aattccagat agggagtggt cgctatacaa
     9301 aaagaattga ataaaacagg atttgttttg ttggagtccc ttacagcgct tatgatcgct
     9361 tctgtctgtg tctacttagt gagctttggc cagctgtttt tgcttcaggc agaaaagaaa
     9421 agtggggagg aactgctatt tattcgaatg atttatgaag atatcaagac gcagcggatg
     9481 tatcacaaaa gaccagagcg aagggaagaa atgatcccat acgatatcca aattgtcgat
     9541 aataatggag taatcgaaaa agtgtttgtc aatatggaga aagaagcgat cagtattgaa
     9601 aaaacaagta attccagcat ttacgctgat tgaatgtcta attggactga tgatattgag
     9661 cagtctattg ctcacattca atcttttgat tcagcaaacc atccaagtaa gtgaattttt
     9721 gcagcgtaaa gatcaaaaag agtggctaat atttttagca caattagaag aagaactcaa
     9781 gagcagtcac aatgtctctg tgaaaaatca gcaactgcac tacacgatcg aggataaaca
     9841 gtatatcatc gaaagatatc aggcaatgat acgtaaaaga agaacttccg gcggacatca
     9901 acctatgtta acaagtatca ctgagcttca attattggaa aaagaacaca caatttcttt
     9961 ttatgtacac tttgaaaatg gagaagatgg atatgccata tggacaaaaa acgatcagta
    10021 gaaaatgtta taagggctct attttatttt ctgtcttgct tttatttctt ttatgtacgt
    10081 ttttattgat ggcagcatta gaaagctacc gtttgtcaat ggatcttaat atacgaatca
    10141 aaaattatta tatagcaaaa ataatgaagc agatggcttt atcggaacag tctacgcttg
    10201 ccgaaagttc ggaaggagtt ttttactaca caactggatc tgtaacatat cagtgggtac
    10261 aacagtccct attcttagag gtggaagtct caccgtttat ctttcggttc acagaagaag
    10321 taaaaaatga tacagatact agtacagaat gatggaatgt tcgtgaaaac gccctctttt
    10381 ctgttataac actgttttta ataaaaaaat gtttgccgtt tcacaaataa aaaacagtga
    10441 aaacgattga aattttgtca attgttcgat agaattaaac aggtgaaaaa attgttataa
    10501 tatatctaga aatgaggtgc accatgttac cagaaaaaat ggaacaaggg tttaatcaaa
    10561 acctggaagc tattcagctt ttacagaatg cattgggaac ttccttttta gaagcctatg
    10621 tcgaaaatgc agaaaattta atagatgatt atcaagtacg tgtggtggat ggagtgccga
    10681 caaaagagac aacgcaacgg atcacagctc tttatgaaga gttgaagaaa ctttctttcg
    10741 agccagaaga atggcgcaga ctgtctcaat tacttttatt gaagggcagc cagacagaac
    10801 atttgcaggc gaaccatcaa ctcactcctg atagtatcgg ttttttattt gtttttctta
    10861 ttgaacaact atacacaaat aaaaaagcac cggttaagat tctggatatt gcggcaggaa
    10921 tgggcaactt gttattaaca gttcttttaa acctatcgaa tgcggggtac caaacagaag
    10981 gaattggtgt ggatattgat gatacattgt tggctgtcgc agcttctaca agtgatttga
    11041 cacaagcaaa tgttcaatac tttcatcaag acgggttgca ggaattatta atagatcctg
    11101 tcgattttgc aatcagcgat ttgccaatcg gttattatcc gaatgacgaa aaagcaaaag
    11161 agtttttaac aagtaccgaa gaaggccata gttatgcaca tcatttattg ctagagcaat
    11221 caatgaaata tgtgaagccg gatggttttg gtttgttttt gatgccgagt ggatttttag
    11281 aaacggatca aagtgaagaa atcaaaaaat ggtttaaaga agaaggttat cttcaaggaa
    11341 tgattcaatt gccagatgaa ttgttccgta ataaacaatc gcaaaaaagt attttgatct
    11401 tacagaaaaa aggaccacaa gcacaacaag taaaagaagt cttgcttgtg aaacttgctt
    11461 cattaaaaga accagagaag gtgacggaat ttttcaacga gtttaaaaac tggaaatcat
    11521 ctgctctgta atcattcaac gagaaggaga gtcatatgtc taaaacaatt gcaatcaatg
    11581 ctgggagttc aagtttgaaa tggcaattat atcaaatgcc agaagaaacc gtagttgcga
    11641 aagggatcgt cgaacgtatc ggtttacaag attctatttt taccatcaag tacggcgata
    11701 atcaaaaatt cgaacaaatc atggatatcg aaaaccacga agtagcagta aaaatgctgt
    11761 tagatcagtt gattgaactg caaattttaa gctcttatga tgaaatcacc ggagtagggc
    11821 accgtgttgt tcacggagga gaaacttatg gggattcagt agtgatcgat gagaaagtaa
    11881 tggaccgcat tgctgaatta gcagaatttg ctcctttgca taatcctgca aacttaatgg
    11941 ggatcaaagc gtttaaaaag attttacctg acattttgag tgtggctgta ttcgatacat
    12001 ctttccactc aacaatgcca aagcaaaatt atttatatag cttaccggtg tcttattatg
    12061 aagatttcaa agtaagaaaa tatggcttcc atggcacaag tcatcgttat gtgtcggaac
    12121 gtgcggcaaa attattgaac aaaccgatcg aagaactaaa aatcatcact tgccacttag
    12181 gaaatggtgt atcgatcaca gcagtcgatg gaggtaaatc catggatact tctatgggat
    12241 tcacaccatt agctggggtg acaatgggta cacgttcagg tgatatcgat ccagctgttc
    12301 tcccatattt aatggaaaaa ttagagattg atattgatga aatgatcaat gttttgaaca
    12361 agaaatctgg tctgctaggc ctaacaggat tgtctagtga tatgcgtgat cttgaaaaga
    12421 actatgaaaa agaacatatt cgtttagctt atgacatttt cgtggaccgc atccgaaaat
    12481 acatcggcgg ttatgtgaca acgatgaatg gtgttgatgc aattgtcttt actgctggta
    12541 tcggcgagaa tgacggacat gtacgtagtg aaatcatcaa aggtatgtct tggtttggtt
    12601 gtgaaatcga tccagcgttg aatgaattgc gtggtgaaga agtagatatc tctacaaaag
    12661 aatcaaaagt aagagtatta gtgatcccaa cagatgagga actaatgatt gcccgtgatg
    12721 tggagcgtct gcgcggctaa gccaaagcat tcgttaggat aaaagatgaa acgcatgcaa
    12781 gagggaattc ttgcatgtgt tttttttgcg gagaagaccg caaaatggta atttaacttc
    12841 aatttccctt cttttttaca ggatcttctt agggaatttt gcttggaaaa gtggtcaaaa
    12901 gggaaaagac tggcatttta tttattgatt ttcaggaaaa tcccaatatt ttattttcat
    12961 tcatctttat aagggacatt acatatgtaa aaagaaaaaa catttttaga tcagcctcgt
    13021 gggctgtcga ctttattctt cgctttatgt ttagctagaa aaccgatccg aaacattcat
    13081 tgatcgaata ataaaaactt gattttccac ctgtactttg cctgaatcgt gaaagcacag
    13141 gtatgaaaat caagtttttt gttgcgaaaa aacgtcctag tcaatcagtt ggccgagcgg
    13201 ctggcggctg atctgctcct gatcaataac gagtgcttct tggaagtgtt gctgcaaaaa
    13261 gtagcggtca tggctgactg ctaggagcgt tccttgatag tcttgcagca tttcttcaat
    13321 ttcttctcga gcatagctgt cgaggtgatt ggttggttca tccaaaatca ggacgttgat
    13381 tttttctaaa aagagtttca gcaacgccaa acggattttt tcaccaccgg ataaatcttt
    13441 gatccgttta cccacatcgc tttgataaaa gccacttttg gctaattgcc gtcgagcgac
    13501 ttcttcgttt ccagtcaatg tgatggcgta agccagcaaa cggctatcgg gatcggcaaa
    13561 ctgcagctgc tgcggcaaat aaccgatttg caagctacta cctaatttga tggtgccacc
    13621 gtaaggcttt tcctcaccga gtaataattt gagtaaggtt gattttcctg caccattgtc
    13681 gccgatgatg gctaaatgat ctccacgata gactgcaaaa ctgctgtctt tgaatagtac
    13741 ttgatcagcg tactgctggt gcaagttttg aatgatgaaa acttctttgc cagattttcc
    13801 accattagac aagctttgca gccgattttt cgggggaggt ggcggtttga cgagtgtcag
    13861 tttcgccagg cggcgttcca gttctttggc ttttttgaag aaggactcat tgtctccttc
    13921 atgtgcccac tgacgaaaac gacgaatcat gactttcaac cgctggactt ctttttgctg
    13981 cagttcgtaa ttttttgtca gctcagccaa gcgagcttct ttcaagactc gaaaacggct
    14041 gtagtttccg ggatactcaa tcaatgcacc ttcttccagt tcccatgttt ttgtggtgac
    14101 ttgatcgaga aattgccgat catgagaaac gaccagaacc gatttttttg tattctgtaa
    14161 atagttttcc aaccagcgaa tactgttaag atctaaatga ttggtcggtt catcgagtaa
    14221 aagtaaatcg gcatcggctg ctaaaatttt ggctaaagcg actttaactc gttcgcctcc
    14281 gctaagttct gttaaccgtg cttcctgacg gtgtggaatc cctaagcctt gcaagatcgc
    14341 tgcaatccgg tcttctaata cgtaaccgcc tagttcttca aagcgatgct gcagctcacc
    14401 gtaacgggcc agacacttct ctaagtttgt atctggttct gtcatcaatg cttcgtagcg
    14461 actcatttgc tgtttgaggt cctgtatctc agcggcagaa tggagcaagt agtggttgac
    14521 ggtcgcttcg gaaactgtaa gttcttgttc gaccgtcccg atctttaagc cttttttgcg
    14581 actgataaca ccgctgtcga caccttctct gccggtcaag atttgtaaaa aggttgattt
    14641 gccagttccg ttgataccga ctaagacaat tttttcacca gcttcaattt gacaggataa
    14701 atcctcaaat aatggattcc cgccgattgt ttttttaata gaaattgctt gtatgatcat
    14761 gatctctctc cttgagacca ctcaaaaagg ccatggttat ccatggcctg tgttatttaa
    14821 actcagatta tcatgagaaa aataccttga gatggccatt gattttacgt gaattcaact
    14881 gttttggaca aacttgtccc gtcaacagca actgtaaaac caattgcatg gctaaatgcg
    14941 atatggtatc gttggtaatt ttcattactt gttattttag tcatgcgcat catagtattt
    15001 tccctccttc gtgtagttgt cttttccagt gtacctgaaa gtgtcagaaa gttcaactag
    15061 cgctgacatt tacttaatat ttatcgatgt gtaattctgt cagagagctt tttttatctg
    15121 actgagggta atgtctcgtg gaccacatca ttgtattctg agctaaacca gcgttcaggc
    15181 gaaatcatat ggtgtttgat aaaaaacttt gtctacatga cttccttttg ccatttgctt
    15241 aataccgttg cattaaacag ctcttctctt tggaaatctc accaacatcg cgttcaggag
    15301 tatcgttgtg gttgcaatgg caagcggtag cctttcatca attcttgaat gatttaccga
    15361 atgtagcact tgccttctcg cggtacccga acccgaaata aaattttagt ttggcgataa
    15421 aatcggtatc cgccatcgat acttcatatt ttttcgtatc agattcatcg gaaacttcca
    15481 cattgacgaa ccaataagct ttggcatgct taggtcgttt taaaccacac cagggcgact
    15541 aatcgccctg gtgttcactt ttttttattt tacttgaact cattgactgt tttcccttta
    15601 ttttaaacat tataaagacc gatgagtgct tgccgcgtct tttgttttta tcacggtttt
    15661 ttgtgcaaaa aaacctgccg atagttggca ggtcgtctac gtgttaagct ttggtgtggt
    15721 ctttagctgg caattggttt tctaaatcag ttcggacaac aagcacatcg caagctgcgt
    15781 ttctaataac atattcagaa acagaaccaa taaataaacg ttctacagca ttcaatcctg
    15841 tggctcctaa cataatcaaa tctacttgat tatcttctgg gatttctctg gcaataattt
    15901 gttttggtga gccatattct acgacagagg ttacattatt caagccagct ttcttcgcat
    15961 tgctttcata atctgccaat gtttgctttg ccatttcagt agcttgttct gcaagcattc
    16021 catcaaagga tgaaacggtt tggaaagaac gtgtgtcaat gacatgtgct aatagaagtt
    16081 ctccgttatt tcggatagct acattgaccg cctttttaaa agctaattca gcttcatcag
    16141 aaccatctac ggcaaccatg atttttttgt attgttgtaa cataagcatt cactcctcaa
    16201 gtcaatcgta ttgtactctt ttattgtaga acactttagg gaaaaagaga attatttact
    16261 gctttggttc agcaattttt tttgcaaatc ggatactgaa ggtggcagaa acaataatta
    16321 cgatcagtaa aaagaccaag gcaataaagc gttgattgaa aaaagctgtt aaaataccca
    16381 ttacgcctaa aacagcatat gcagctccgt aaaattgtag gaatccttga tttttatcat
    16441 ttttgttgat caatacaaaa aagatatcct gtttattaaa taaataatag ctaacgaata
    16501 ataatactaa agtggcgata atcattaaaa tcctaatcat gaacaaatcc tccaaaataa
    16561 gcaacaaata gaaaaagcaa tttgccaaga gcaaattgct ttattcttta aaagatggta
    16621 atctccgtta atgccgttgt ttgtcctgta gtattgatcc cgcgaattca agcaggtggg
    16681 tcccatggtc gtagcttcga actaccaaat gataaggcat gttaccagca gtgacacaag
    16741 acgggtccca agatatcaat aaaaatgttc ggtcaacaca caaagaggac aattcgaaca
    16801 tcacagattt cccacctcta tagtagcata tgaatttaaa aagagcaatg agagagctcg
    16861 cagaaacaga accgttttct tgtctagttt tttggaggat gctttttcca ctcttttata
    16921 cggtaatact gttgacccag agcctgttca tatttccctg tatcttttgg ctgataatac
    16981 tgagcattct tcagtttatc tggtaaatat tgctgattga cccaagcctg atcaaaatga
    17041 tgaggatact gataacccac gccacggttg agtgatttcg cacctttgta atggctatct
    17101 ctcaaatgat caggaacatc tccagctttg ccctctcgaa tatcagcaag tgctgcatct
    17161 aatgccatgt aggcagaatt ggattttggc gacaagcaaa gatcgacgac gacgtctgct
    17221 agtgggatac gtgcctcagg caatcccaat ttttcagcag ctaatacagc gttcactgta
    17281 cgagccgcag cagcaggatt acctaaacct atatcttcat aaccgataac catcagacgc
    17341 cggcaaatac ttgccagatc acctgcctca actaaacggg cgagataatg aagtgcagca
    17401 tccacatcac ttccacgaat cgatttttga aaagccgaaa taacgtcata atgagcatct
    17461 ccatttttgt catgggtaag agcttttctt tggacacatt cttcaatgat cgacagtgta
    17521 agatgaatcc ttccttcttt atctgaagga gtcgatagtg tagccagttc cagcccattc
    17581 aatgcactcc gcaaatcgcc gttcgttgca cgcgaaaggt gaagaagagc atcttcgtct
    17641 aactggattg cttgttgtcc aaggccgcgt tctttatccc ttaaagcatg ttcgactgcc
    17701 aactggatgt cttgttctgt taatggtttg acttcaaaga tctgtgtacg gctgcggatc
    17761 gctggattga tggtaatata aggattctca gtagtcgctc cgatcaagat gattcggcca
    17821 ctttctaaat gaggaagcag aaaatcctgt tttgttttat ctaatcggtg tacttcatcc
    17881 aataagagga tcactgtacc actcattttt gcttcttccg ctacaacttg tagatccttt
    17941 tttgaatctg tagcggcgtt tagcatgcga aatgcataat tagtagagcc ggcgatcgca
    18001 cttgcgatac tcgtttttcc agtaccagga ggtccatata gaatcatgga agagagcatg
    18061 cgagcatcga ccatgcggcg tatgattttt ccttccccga ctagatgctg ctgaccaacg
    18121 acttcatcga tcgttcgagg tctcatgcga taagccaatg gctgttgcat aggattttcc
    18181 tccttcttgt ttttacgtcc cttaattata tcatatttag caagatgcct agaaagaaca
    18241 gccttctgtt gttttgtttt catagcttat ccaactaata ttgtgtatga taaagtaggt
    18301 gagaaaaatg acggataaac aaaagataga tgaatttttt tggaataaaa cgacgccaga
    18361 agtggcacgg gatttattgg ggatgtattt ggagcatgac acaccagaag gaagattagc
    18421 tggatatatt gtagacgcgg aagcatacct aggtccagaa gacgaggccg cccatagcta
    18481 tggtttaaga agaacaccgc gtgtgcgggc aatgtatgaa aaaccaggta cgatctacct
    18541 ttacacgatg catactcatc ggattttgaa catcattact cagccagaag ggattcctca
    18601 aggagtaatg atacgtgcaa tcgaacccgc cgcaatgatt gatcaaatga gtaaaaatcg
    18661 tggcggaaaa actggtccag atatcagcaa cggtccggga aaattagtgg aagcattagc
    18721 aattcctcaa gaactttatg gtcagtctat agcagatagt tcgttacgct tagtttttga
    18781 gaaaaaaaag acaccaaaaa agatcaatgc tttgcctaga atcggtatcc caaacaaagg
    18841 tgtatggaca gaaaagccgt tgcgttttgt tgtgtcggga aatccttata tctctttaca
    18901 aagaaaaaac caaatcgaga aagattgggg ttggaggaaa gaaaatgaaa aagaaggatc
    18961 tattaacata tttagatgaa ataattgaaa aacaagtgac agactatgat gtagctttag
    19021 attgggatac aaaaaatcat acgatcgaga tcgttgttcg cttgtttgca gaaaataaag
    19081 cacaatttga gattgatgat gcagaaggga tcgtttcgca agaaccaatt atcgaatttg
    19141 aagacggcgt acttcttttt aatcctcaga aatctgtttt tgatgaacgc aattatctag
    19201 cagtaatccc ttatgaagga aaaaaagggt tggcaaaatc agtagctgat tctttagtgg
    19261 aatatctaaa tgaagtatta gctcaagggc aaagcgattt gcttgatttt ttggatgaag
    19321 acgatgacga agctgtattc gagcttcact gggataatca gcaactagca gaattagtag
    19381 aagtaaaaca gcaaaatgaa gcagatactt atttaccgta cccaagttat taaggagtga
    19441 cagatatgaa atggaatgaa gtaaaagtag aaactgaaag tgaagcagtc gaagcagttg
    19501 ctaatatttt gatggaggca ggggcgagtg gtgtcgcgat tgaagattct ttagatgttg
    19561 aacattttca gtcggatcaa tatggtgaga ttttagataa aaatgatttt acatcgctga
    19621 aagaaggcgc actagtcatg gcttattttc cggaaacgac tttcttaccg gaaatcttgc
    19681 cttttatcaa agatcgtatc acacaattac ctgagtttgg tctagctatt ggaaaaaata
    19741 aagtaacagt tagtgaagta gaggaaagca actgggcaac agcctggaag aaatactatc
    19801 atccggtgcg tattacgcga atgctgacgg ttgcgccaag ctgggaaaac taccagacag
    19861 aagatcctct agaaaaaatt atcacactag atcctggtat ggcatttggc acaggcactc
    19921 atcccacgac ttccttgaca ttgacagcac tagaaacagt cttgcgtggt ggtgaaacat
    19981 tgctagatgt gggaactggg tcgggcgtgc taagtattgc agcaaaatat cttggtgcaa
    20041 aagatgtcta tgcctttgat ttagacgaag tagccgttcg ttcggcgaaa gaaaatatgg
    20101 atatgaatga aatagccaaa gatgtccatg tatcagctaa cgatcttttg acaggaatcg
    20161 agattgaaag tgatgtaatc gttgcgaaca ttttagcaga tatcatctta ttgatgatcc
    20221 cagatgcttg gcgtttactg aagcaaacag gcacgctgat cgtttcagga atcattgaag
    20281 agaagaagca aatggttctg gatgccatgt tagaacaagg gtttatcgtg gatcaaattt
    20341 tccagcaaaa agactggtat gcaattattt tgaaaaaacc agaggtggaa tagatgcagc
    20401 ggtatttttt aaaagaatca tatgaatcaa aagactattt tgttttagaa ggggaaaact
    20461 atcaccacat tgtacgggtt atgaggatgg aacccgagga tgaagttttt ttggcattca
    20521 atgatcaaat agccattgtt gcagaaatca cggagattac tgaagaggct gtttatctga
    20581 aagaaattaa aaaagaagaa aatcaaaagg aactgccaag ttcgattacc atagcgagtg
    20641 gctatcctaa aggggaaaag ctggattgga tcgttcagaa atccactgaa ttaggagcgc
    20701 aagcttttct tggtttcccg gcatcagcct ctgtcgttaa atgggatgca aaaaaacgta
    20761 tgaaacgaca acaacggctt gaaaagattg ctcaagaggc agcggaacaa tcccatcggc
    20821 agatcgtgcc atcagtagag ttattggaaa cgaaacaaga tttatttgag cggctagatg
    20881 attatgacgt ggtcttggtt gcttatgagg aatcagcaaa gcaaggcgaa aaatcaaatg
    20941 cagccaaaat tcttagtgaa acaaaaccag atagtcgaat cttagctgtc tttggaccag
    21001 aaggtggatt atcccgcgat gaagtcgatt attttgtaga acacaaaggg atactttgtg
    21061 gattaggacc aagaatcctt cgtacagaaa cagcacctct ttatttatta agtgctgcta
    21121 gctatcaatg ggaattaatg aatccttctt tcagctaacg ttcttcatta ataaaagagt
    21181 atcgacagaa agaattgaaa aaatagtcaa caatttgtat aatagaaaca attatttata
    21241 caatggatac gaaaaatcgt ttgaaaaagc ttttaaaaag cgaacatttc cgtaaaaaaa
    21301 cgtataactg cacggatttt gggacaatta tcaaacagat atttgtcccg attcccgttt
    21361 taccgaaaaa agaaaggggg atcgattcct gtgacaaaag aagaaatttt aacgggaccc
    21421 ggtgtcataa agctcgttag tcaatacatg ggtcccgaac atgtagcatt tgttgagaaa
    21481 gcatgcgaat acgcgactgc tgcacatgac ggacagttta gaaaatcagg cgaaccctac
    21541 attattcatc ctatccaagt cgcaggtata ttagcagatt taaaaatgga tccccataca
    21601 gtggctacag gcttcttaca tgatgttgtt gaagatacag aaatcacttt agaagatctg
    21661 agagaagaat ttggcgatga cgttgctatg ttagtggacg gcgtaaccaa attagggaaa
    21721 ataaaatata aatcccacga agagcagcta gcagaaaacc accgaaagat gctgctagca
    21781 atggctcaag atttacgagt tatcatggtc aaattagctg atagactaca taacatgcgt
    21841 acgttgaagc acctgcgaga agataagcaa agaaggatcg ctcaggaaac tttagaaatc
    21901 tatgcgcctc ttgcacatcg tctagggatc agccggatca aatgggaatt agaagataca
    21961 gcacttcgtt atctaaatcc aaaacagtat taccgtatcg tccacttgat gcagacgaag
    22021 agagaagaac gtgaaaaata cgtaagtggt actgttgaag atattcgaat agcgacagaa
    22081 gagctgggga tttttgcaga aatctatgga cggccaaaac acatttattc aatctatcgt
    22141 aaaatgaaag atcagaaaaa gcagttcaac gaaatttatg acttgctagc tattcgagtg
    22201 atcgtagatt caatcaagga ttgttatgct gtattaggag caatccacac aaaatggaaa
    22261 ccaatgcccg gcagatttaa ggattatatt gccatgccaa aagctaatat gtaccaatct
    22321 ttgcatacta ccgtgatcgg tccggcagga aatccggtag aaattcaaat ccgaacacag
    22381 gaaatgcatg aaatcgctga attcggggtt gctgcacact gggcctataa agaaggaaaa
    22441 aatgaaaaag tagaaccaga tggtatgacg aaacaattaa gctggttcca tgagatactc
    22501 gaacttcaag acgaaagcta tgatgcttct gaatttatgg aaggcgtaaa aggagatatc
    22561 tttagtgata aagtctacgt cttcacacca aaaggagacg ttactgagtt accaaaagga
    22621 tccggaccat tggactttgc atacagtatc catacagata tcggtaacaa aaccactggt
    22681 gcaaaagtaa atggcaaaat ggtgcagctt gattacaaat tgaaaaacgg agatatcatt
    22741 gagatcatga cttctccaaa ttcatttggc ccaagtcgcg actggttgaa attagttgct
    22801 actagcaaag caagaaataa gatcaaacgt ttcttcaaag cccaagatcg agaagaaaat
    22861 gtgatcaaag gccacgaatc cgtggtcaaa tgtattacag atctaggatt tacgcctaaa
    22921 gatattttga cgaagaacaa actgcaagaa gcactcgatc gttttaatta tcaaacagaa
    22981 gatgatctct atgcagctgt agggtatgga gaagttagcc ccttgacgat ggccaatcgt
    23041 ctgactgaaa aagaacgtaa agaacaaaaa atcgagcagc aaaagcaaga agcagaagaa
    23101 atcatgaatc agccgaaaaa agaacctgac aaaatgaaag tacgtcatga aggtggcgtt
    23161 gtcattcaag gggtagaaaa cttactaatt cgtatcagtc gctgctgtaa tccgattcct
    23221 ggtgatgata tcgttggtta tatcactaaa ggcagaggga tatccattca tcgtcgagat
    23281 tgtccgaatg ttcagcctga caaaccaaat gtagcagaac gtttgattga agtcgaatgg
    23341 gaagatacat cgaatacacg aaaagagtat gatgcagatt tggaaattta cggctataat
    23401 cgttcaggct tattgaatga tgtacttcaa acagtcaatg cgctaacgaa aaatctcaac
    23461 agcgttgaag cacggacgaa taaagataaa atggcgacga tccatttgac ggttggtatc
    23521 cagaatttat cccacctaaa gagtatcgtg gataaaatca aagcagtacc tgatgtctac
    23581 agtgtacgcc ggacgaatgg ataggaggac agatatgaga gcagttgttc aacgggtaag
    23641 taaagctgct gtgtcagtcg atcagcaaga aattggaaaa attggctacg gcctacttgt
    23701 tttattaggc attcatgaaa ccgatacgca aaaagatgtg gactatctgg taaaaaaaat
    23761 tgcacaaatg cgtatttttg aagatgaaca aggaaaaatg aatattagtg tagaagatgt
    23821 gggaggacaa attttaagta tctctcaatt cacactgttt gcggatacga aaaaaggaaa
    23881 tcgaccaagc tttgtggaag ccgcacgacc agaaacagct attcctttat acgaatcatt
    23941 caatgaagga atcagaagtc gcggcatcac agtcgctacc ggacaattcg gagcagatat
    24001 gtcggtttct ctcgtcaatg atggacctgt aacgatcatt attgattcta aaaacaaata
    24061 aagtaatctg gttttcacta gtaaaaacta tgaaacacgt cgcttaagtt agactgataa
    24121 actctaactt aagtggcttt tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa
    24181 aaaagttaag cttaaattat acaagttatt ttttatagga ggttgctgaa gatgaaagaa
    24241 gtaccagtag tagatatcaa aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac
    24301 atgttggagg agttacgcca tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat
    24361 aaagaagcta tcacgatcta cggatcatca gcagcaaaag tattttatga tccaaggaat
    24421 ttcaaacgaa aaggtgctat gccaaaatta gtattgaaga cactttttgg tcaaggtgga
    24481 gtacagactt tggatggagc tgctcatcac catcggaaaa atatttttat ggatctaatg
    24541 acaccggaaa gaatggaaga ttatcatcgt atcttagata agaatctgac acaagcactt
    24601 gaagcacagc atggtcaatt tgaactattt gatttaagta agatggtttt ctttacatct
    24661 atttgcgagt gggcaggtat caatttatca gcgatttcta aagatgaggt cgaaaaatta
    24721 gcagaatatc aaatctctat gatcagcgga acgtttactt ctccgattga tcacataaaa
    24781 ggtgtcgaaa atcgcaaaaa atcagaaaaa tgggcgcaag gcttgattga agaagctcga
    24841 caaaatcctg tggctggaaa agaaaatgtg gcattatatg cttttgccaa tgccacagat
    24901 ttagatggtc agcttttacc tctggaagtg gcagctgttg aattattgaa catcatccgt
    24961 ccaacggttg ctctaactgt gtgggcggca ttgatgggac atgcactttt cagtagacca
    25021 gatttgtatc agcaattaaa aaatgacttt tcgacgttac aagatccgtt tattcaagaa
    25081 atgagacgct attatccttt cttcccaatg ctacctgcga tttcgctgaa agaagtagaa
    25141 gtagatggtt atcgtatacc agaaggcagt tgggttattt tagatttata tggaacagac
    25201 catgatgaac gaacagttga agcgccagat tcctttatga tcaaacggta tgtagggaaa
    25261 gcaaaagata tttcctataa agaagaatat gagatgattg cacaaggcgg gggaaatttt
    25321 cgacaaatgc atcgctgtgc aggagaatgg atcactcttc atagtctacg tgtgttttcc
    25381 gatcagctag taaataagtt tgagttctct gtacctgaac aagattggac gattccattc
    25441 aatcagtttc cgacttatcc gaatagccga gcgctacttt acaagaatta gattagcata
    25501 aacttatcga aaaaaaggaa gatcaaacag tgctgatctt ccttttttca tattactata
    25561 aattgataag gtcaattgat caaaatccac gaaatgcttc atcaatggtt tgataatctt
    25621 cattggataa atgcaaatct aaagcttgga cattgtcaga tacttgctct ggacgcttag
    25681 ctccaggtat aactacagac actcgtggat tcttcatgta ccaagctaaa acaagttgcg
    25741 caggagtggc ttggtatctt ttagcaatgg ggcgaagtac atcgacagcg gttaaaattt
    25801 ctctgaaacg ttctccttgg aaatctggat ttctttttcg aggatctcct tcaccaaatt
    25861 gtttttcctc accaagttca tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg
    25921 gaacaaaact gattttgttt ttttcaagat aagggaataa ttctttttca gcctgccgat
    25981 ggatcaaact gtatttatct tctactacat cgacatatcc atctgcattc gcttctttca
    26041 attgttctaa cgtgaagttt gacacaccga ctgctcggat ttttcctgct tcttttaatt
    26101 cgtgcaatgt agcaactgat tcattttttg gtgtgctttc atccggaaag tggatataaa
    26161 aaatgtcgat gtaatcagtt tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa
    26221 actcaggaga attatcaaat gttcgccctt tatttggtac atgagcagct tttgtagcga
    26281 tgacgacacg tgagcgatca tattcttttt ccttcagtac ctcgccaatc agttcttcgg
    26341 aacggccgtt tccgtaagca taagcggtat cgatcaaatt gatcccgcta ttcaaagctg
    26401 tacgaactac ttgttttcct gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa
    26461 cggcatttgc tcctaatcca agctgttctg cgtagacttg tgaatgtccg attcttattt
    26521 ctgccatagc tgattcctcc tttttgtcta gtctaaaaca aaataccaaa aagctgaact
    26581 aaaacgtttt tgtgagggga aatagtcaga tatctcttga ctttgagact ggtttttagt
    26641 aggttataag taatatcgaa tgtcaatgaa agagcgagta agtttttgaa actttacaag
    26701 agaggatcat cgaggctgaa aatgatctaa agtttcatta gcctaaagac actctggaga
    26761 tggacgagtg aaaagccgtc ccgcgtacga gcgttattcg taagaggaaa gagaaatctt
    26821 tcaaaattag gtggtaccgt gtatgaattt cattcaccct tgttcatgat gaacaagggt
    26881 gattttttta tttacatagc taagaaaggg agagaaaact atgagtttcc aaagaccaaa
    26941 aggaacaaat gatattttgc caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc
    27001 acgtttatta ttcagagatt atcagtataa tgaaatgcgt acaccgattt ttgaacacta
    27061 tgaagtgata tcccgaagtg taggggatac aacggatatc gtttcaaaag agatgtatga
    27121 tttttatgac aaaggggaac gccatgtgac gcttcgacca gaaggtacag caccaatcgt
    27181 tcgttctttt gttgagcata aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt
    27241 ttatatggga ccaatgtttc gctacgaacg ccctcaaaaa ggacggttac gtcaattcca
    27301 tcaaatcgga gtagaagcat ttggatcaga aaaccctgca actgatgtgg agagcatggt
    27361 gatggcactt gatttcttca aacagttggg gatcacgcaa atccggctag tcatcaattc
    27421 attaggtgat aaagaaacgc gtcaagccta tcgtcaagca ttgatcgact atcttttacc
    27481 ttttgaatca gagctgagtg aagactctaa acgccgcctt catgaaaatc cattacgcgt
    27541 attggacagt aaagacaaac gagatcaaaa atttgtggca gaagctccat ctatcttaga
    27601 ttatctaagc gagcctgctc aaaaacattt tgataccgtt gtttccatgc tggatgcatt
    27661 ggctgttcct tatgaaatcg acagtaatat ggttcgcgga ctagattatt acacacatac
    27721 gatttttgaa atcatgagtg acgcaccaaa aatgggggca caagcaacga tttgcgcagg
    27781 cggacgttat gacaatctag tggaagaact aggtggtcct tcaacacctg gcttcggatt
    27841 tgcaatgggt atcgagcgat tactcttgac tatggaggca gaggaagtag ttatccctgc
    27901 atttaatgag ttggatgctt acgtggtcgc actcggagac gaaacaaata tcgaagcatt
    27961 gaaagtggtc caagcgattc gaaactttgg tttttcagct gaccgcgact tcatgaaccg
    28021 taaagcaaaa gcgcaattta agacagcaga taaagcgaat gccaaattag tattggtgat
    28081 cggtgaagat gagcttgcta gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga
    28141 aaaaagtttt ccattagaag agatatatga acattttgat gaagtctatg atgaaatgac
    28201 tttattcgag taggaggaaa aaaataaatg gcacaaagaa cgatctattg cggattagtt
    28261 tccgaacaac aattagaaca aacagttaca ttaaaaggat gggtacaaaa aagacgagac
    28321 cttgggggag taatctttat cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt
    28381 aatcctgaaa tcaacaaaga agcatgggaa atcgcggata aatgtcgcag tgaatacgtg
    28441 atcgaagtga ctggggaagt tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc
    28501 actggtgaat tcgaagtgat tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg
    28561 ccatttcaaa ttgaagatga tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat
    28621 ttggatctgc gtcgtccaaa aatgactaaa aacatcaaat taagaaatgc gacaacaaaa
    28681 gcgattcgtc attatttaga tgataatgac tttctcgata tcgaaacacc ttacctaggg
    28741 aaatctacac cagaaggcgc aagagactac ctagttcctt cacgtgtcca tgctggacat
    28801 ttctatgcgt tgccgcaatc tcctcaattg tttaaacaat tgctgatgaa tgctggattc
    28861 gaccgttatt atcaaatcgt tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag
    28921 cctgaattta cgcaagtaga tattgaaaca acgttcttaa cagcagaaga aatccaaact
    28981 tacacagagg gattgattgc gaaggtaatg aaagaagttc gcggtatcga agtcacattg
    29041 ccatttcctc gtatgaccta tgatgaagca atggctcgct acggcagcga caaaccggat
    29101 acacgttttg atatggaatt gatcgatcta agcgatacag tcaaagaagt agaattcaaa
    29161 gtcttccaaa tggctcttga aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct
    29221 gcagatcgct actcacgaaa agacatggat cagttaggtc aatatgtagg acaattcgga
    29281 gctaaggggc ttgcatggct gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa
    29341 ttcatgggag aagcaacaga agcaatcatt aaagcaacag atgcaaaacc aggtgacttg
    29401 ctgatgtttg gcgcagataa aagtgaaatc gtcgctgctg cattaggtgc tatccggaca
    29461 cgtttaggaa aagaattagg actaattgat gaatcgaaat tcaatttctt atgggtaact
    29521 gattggccac aatttgaatt ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca
    29581 ttcacgatgc caaaagaaga agatatcccg ttattggctg aagaccctgc aaaagtctat
    29641 gcagaagctt atgatatcgt cttgaacggt tatgaactag gcggaggttc tatccgtatt
    29701 catactcgtg agcttcaaga aaaagtacta gaaactctag gctttacaaa agaacaaatg
    29761 gaagaacaat ttggtttctt gttaacagct ttagattacg gattccctcc acatggcggt
    29821 attgcattag gattggaccg ttttgcaatg ttattagccg gagaagataa tattcgtgaa
    29881 gtcattgcct ttcctaaaaa cggaaaagca gcagatgtga tgagtgatgc accaagtatg
    29941 gtttcgcctt tacaattgtt cgaattgaat attgatgtaa cgaatataga agaatagccg
    30001 ataaaaaggt aaaaaggatt tgtcgaatac gcttcttttg acaaccttga tttaattgaa
    30061 aaacacttta taatgaagcc ataacatata aagcggggct tttccatgaa aaaaacattc
    30121 gaaaattggg cttatcatga ttacacgacc aagctttcgg tatctatcgt ttatgcgttg
    30181 atcgcatcag tcgcattgaa tttcttttat cagccaggac atatttattc aagtggtatt
    30241 actggtctag ctcagatttt gacgactttg tcaaaatcac tgataggatt cgaagtaccg
    30301 gtatcaacaa ctttgtattt actgaatata cctttgtttg tattggcgtg gttaaaaatc
    30361 gggaagaagt ttacaatttt cacttttatc acagtcactt tgacctccct ttgtatccat
    30421 attttaccgg aaacagtcct atctaccgat ccaatcatct gtgcgttgtt tgggggggct
    30481 gtgatgggac taggtattgg gctagcgttg agaaacggcc tatcatcggg cggtctagat
    30541 atcgtgagta tcacgattcg taaaaaaaca ggaaagtcga ttggttctat ttcgatctac
    30601 ttcaatgcat tgatcgtttt tgttgccgga attctatttg gctggcaata tatgttttac
    30661 agtacgttgt ctattttcgt cagtggaaaa gtaactgatg tcgtttatac taaacaaaag
    30721 aagatgcagg taatgatcgt aacgaaacat ccagaaaaag tagtaaatga aatccaaaac
    30781 tgtatgcgta gagggatcac gatcatcaat gaagcagaag gtgcttataa gcatgataag
    30841 cagacagtat tgatgacgat cgttacacga tacgaactgc ctctacttag agaagcaatg
    30901 aaagcttctg atccacaagc ttttgtaagt attgcagaaa acgtccagat acttggtcgt
    30961 ttctatgagg atggcattta gatcgagttt gacaaaaatt catgaaatac tgctgtagac
    31021 agacgttcgt ttgtctgcag tttttttcta ccatagtaga atcagcaaag aaaaactgct
    31081 tttttatttg aaattttctt aaagttgaaa aaaagcatgt ttattttttt aaatttatgg
    31141 gatgatagaa aaaaatagta gagaaaggac acgattctac ttgctatttt cacttaatac
    31201 ccgtatatta cgaatgaaaa aagtttatga aaagttggaa tattgaatgg aaaaacaaaa
    31261 aaggttaaaa aacagccgct acatcactgg gtttgatggg ctccgttcat tagctgtggt
    31321 gggtgttatc ttctatcacc ttctgccaac tagtatgaaa ggcggctatt tgggcgttcc
    31381 gatttttttc gtcgtatctg gttatttgat cacggatctg ctgcggcagg aatgggaaca
    31441 aaatggaaaa atcaatattt ggcaatttta tgtcagaaga atgaagagac tctatccggc
    31501 acttgttttt atgcttgtga cagcttcggc ttatatcact ttgtttcagc gagggctgtt
    31561 aaacaatctt cgaggagttg tggtcagcag cttactgtat gtcaacaact ggtggcaaat
    31621 caataacggt ttatcttatt tcgatcgatt tgctaatgag tcaccgttta cgcatatctg
    31681 gtctttagca gtagaaggtc aaaactattt ggtttggccg atcttgtttg tattgttgat
    31741 ggtatttgtt cgtaaaagaa aatggatctt ttatactgtg ttaggtacat ccttcctctc
    31801 cgccatcttg atgatgatct tgtatacacc aggaggcgat ccaacccgtg tttattacgg
    31861 taccgacact cgtctgtttt ctatatggtt gggaagtgcg cttgcgtttg tttggccaag
    31921 tacgcgttta aagaaaaaca tcccgaaaca agcgaaaaat attctgaata tcgctggagg
    31981 aatctcgctt ttggcactga ttctttgctt cttcttttta gatgatcatt acaatttcat
    32041 ctattacgga ggaatgctcc tagtgagtct gttatgcgta gtgctagttg cagtgacagc
    32101 acaccccgga gcaagtttga atcgatggct gacaaatcca gtatttactt ggattggcaa
    32161 acgaagctat ggcatttatc tgtatcagtt tccagttatg attttttatg aagcaaaggt
    32221 gactgatatt gccgatcatg ttttgttgca tactttagtg gaaatcacat tgattctaat
    32281 catcagtgaa ctttcttatc gatatgtgga gcgtccgcta gcacgttttg attatggtcg
    32341 tacattccaa gtgatgaaag gatggttcag caaaccgatc atcagtaaga gcaaaccatg
    32401 ggttgttcca ggcacattac ttgtagcaat tgcactcgtt ggtttcgtta cggcaccgaa
    32461 aaacgctgta acagccgatc aaaaacaact gcaggcaaaa atcgctgaaa gtaaaaaatt
    32521 agcagaagaa accagaaaca attcagaaaa caccactagt tcggttgacc aagcgatcct
    32581 tgataagtac gagttgtcca aggcacaagg gaataaggca caaaaattag aactaacggc
    32641 atttggtgat tcagttatgt tggatgctgc aacggatcta caagaacttt atccaaaagt
    32701 agtggtagat ggagatgtcg gacgacaatt atatgccagt gtaccgtaca ttgaaaaatt
    32761 aaaggaacaa gatttgttga aagacactgt tctgatcgga ctaggaacga atggctcatt
    32821 ctcggaagct cagtttgata gtgtgatgga tgcaatcggt gatcgaaaag tatactgggt
    32881 caatgtccga gtaccgacga agcgctggca aaatgacgtg aatgcgatgc ttgaacagat
    32941 gacgaaaaaa tacgacaata tgacgctgat tgattggtac agctacagta atgaccatag
    33001 tgactggttt tatgatgatc aagtccatcc aaatcctgaa gggatgacac attacattca
    33061 cttagtcagc gaagccttgc taggaggaag ttcaagtacg aacagcagtg agacaaacgt
    33121 gacagaatga tagctacaaa aaacttcagg caaaaacatt tgtttttgcc tgaagttttt
    33181 ttatatttac cgtgttttta acattcatat agattcagga agcaaaacgg ttgagtgtgt
    33241 ctgtaaataa cgttcttttt gttttcgggt gagttttttg aatgcagctt ctgcttttgt
    33301 tgcttcgctt cttgaagaaa atacttctgc atggatcatc ttaacaggtc ttcttttttg
    33361 aagacgagta tatttagcac cagtaccatt gttatgttcc tctaatcgtc tagaaagatc
    33421 ggttgtatat ccaccataaa atgtctgatc tttacagaat aatacgtaaa aataatgttc
    33481 atttgccata gagcatctca cgaatctctg cagtgtattc tccatccata ttgtaagtga
    33541 tcaatggtgg agcgactttg aatccgtcta atttgccttc tttgattcct tcaactaaca
    33601 aaatatttgc ctcttttcca ggttttgggt aaacaaattg gatcctttta ggtgcaattc
    33661 gatagtcttc catgacctga atgatttcta agaaccgatc aggacgatgg accatagtaa
    33721 agcgtccatt tgtttttagt agcttacttg aaatatcgat cacttcttct aacgtcgtat
    33781 gaatctcatg acgggcgatt gccaggtgtt tattagggtt cgttttattt gttggcagcc
    33841 ctttgaaata aggtggatta cagacgacta agtcacaaga attatgtcga acgacagata
    33901 agctgtcttt cagatcgatg gtgtggacag tcatttgttc atttaaatgg ttaagcgcga
    33961 tactacgtct tgccatgtct gctagacgag gttgaagttc gatagcgtcg atagccgcct
    34021 gggttcgttt acttaaaaaa agtccaactg cgccatttcc tgcacaaagg tcaacaatct
    34081 tccctttttt aggtacacgg gaaaagtgag caagcagcac agcatctaaa gaaaaagaaa
    34141 acactttgga actttggata atcttgatat cttctgcgta taattggtcg attcgttcgt
    34201 cttcgtgaag catggattcc ctccaaaatg aaaaaaatag tcgtctgtat tatactattt
    34261 ttattaaaaa gaaccaaggt ggcttgtcaa acctctattt tttatgcata ctatgaccat
    34321 aagaagggaa aggaagagac tatgttttat cgatttatgc gcggggtagt tcgagtacta
    34381 ttagccttag ttaatggaaa tgcccgctat gagaataaag aagttttacc gcaagatgag
    34441 aattacatac tagttgctcc ccatcgtact tggtgggacc cattatatct ggcggtagct
    34501 gcagcaccca aggaattcag ctttatggca aaagaagaac tgttcaaaaa tccagttctg
    34561 cgatttatat taactcgatc caatgctttt ccagttaaaa gagaaaatcc aggaccaagc
    34621 gtcattaaaa cacctgtaaa gattttgaaa tcgacaaatt tgagtttgat catgtttccc
    34681 agtgggacaa gacatgctac agaacttaaa ggcggaatgg cattgatttc cagaatgtct
    34741 aaagcgaaaa tcgtgccagc tgtatatcaa ggtccgttga cacttgggga tctgttcaag
    34801 cgtcagagag tgacagtgcg tttcggcgaa ccaatcgact tatcagatat caagaaaacg
    34861 gataaagaag gtatggcgga gatcgaacga cgaacacaag cagcttttga caaattggac
    34921 aaagaagtga accctgattt caaatatgaa gtcaaaaaaa aataaaatga taagtttatg
    34981 cgtgggcggt cttttgaatc tactgtcctg ttcagttcag actttttcaa tgaaaacgag
    35041 ggagagacgt caagtctgcc ctctttttgc gttccaatat gtaaaataga agacaggtgt
    35101 catgtaatgg atcaaagtgt tataataatt agaaggacat tttttgaggt gattgaatgc
    35161 gttttttaca tacagccgat tggcatatcg gaaaaaaact acaaggttat gacttgattg
    35221 aagaccagcg tcatgtacta tcttctatac tagatatcgc gaaagaagaa aaagtagagg
    35281 caatcgtgat tgccggagac ttatatgata ggagtgtccc agctgttgat gccgtagaat
    35341 tatacaatga attgatggtc gattggaatt taaaggaaaa gatcccgatt tttgctatat
    35401 caggaaatca cgatagcagt acgagacttt cagcgggtac gccatggttc agttattcga
    35461 actatttttt gcgaacacag ctagcagaag catttgaacc agtcgagttt aaaaacgtac
    35521 aatttttctt tcttccttac tttgaaccaa ttgcagcgag attatatttt gaagatgaca
    35581 atatccgaac gatcaagcaa gcggtagcca aggtgataga taaaatgcag gagctatttg
    35641 attcagaaaa aaaacaagta ttgattagcc atttttttgt aatgggaagt gtaaaatctg
    35701 attcggaaac gaaaatcgaa gtaggcgggt tggattcgat ccctggtgaa ctacttacac
    35761 cgtttgatta tgtagcacta ggacatttgc ataatcaagc tgctttgaga caggataatg
    35821 ctcgctatag cggttcccca ttgaaattct ctttatctga gatcaaccaa aaaaaaggcg
    35881 tatggatcgt tgacctgaca acaggcctgg agttgaattt tagggagatc gaacccctgc
    35941 atgatattga agaaataaca gcgagtttca aagaattgct ggatcctgcc ttttatcagt
    36001 ccgtcaatcg ggagaattac atctatatct atctcactga tagaacgatc atcccgaata
    36061 tgatgaacca gcttcggcaa atatatcctc gtattttggg gattgaacgg gcaaacggtc
    36121 gagcagaaca aacaaagcgc aaacgaaaag tactagaaat gaaagatcct caaaaattag
    36181 cagaagcatt ttttagtgaa atgacggaag agcagctaac tgcaaaccaa gaaaaatggc
    36241 tcggagaaac gttgcaagaa atcatggaaa tggaatgatc aagaatgaaa ccgaaaaaat
    36301 tacaacttaa aaattttggt ccttttatca atgagaccgt agatttttca cgtttgacag
    36361 aagcaccgct ttttttgatc agcgggaaga caggagctgg caaaacgacg atttttgatg
    36421 ggatcacctt cgcacttttt ggtgaaactt ccggaagact tcgctctgga aaagagatgc
    36481 gttctttgtt tgctacacca gacgaagaaa caagcgtgac tttttctttt gaacatcagc
    36541 agatgagcta tgaaatcgaa cgaaaaccag aacaagtttt agcaaaacga aaaggaaacg
    36601 gaacaaaaaa acaggcagcc aaagtaacgt tgaccatttt tgatgggaaa ggaaaagaaa
    36661 taaaacaaat cacaaaacgt acagaagcag atcagttgat cagagaactg atgaatctcg
    36721 atgcgaaaca gttttctcaa atcgttttac taccccaagg agaattccgt aattttttgg
    36781 tttcatcaag cagtgataaa gagactgttt tacgtcattt attcggtact caatttttcc
    36841 aacagttcaa tgagcgatta aaagaaaaag caaaacaaca gcaacaaaaa ttggatcatt
    36901 tagagcaaga gctgcatctt ctgcaaaaaa ggtttgtttt agtcaaagaa gaggaaccag
    36961 cagcagggtt tgaagccgtt ttagcccaat ggacaaacca tcaggcaata ctcgaaaaac
    37021 aaatcacgga agaaaaagaa gctctaacgg aacttcagga aaaacaaaag caactagaaa
    37081 attcctacta tcgttttgaa agcttgaaaa aaagttatga agaaaaaacg atattaataa
    37141 acaagcaaaa ggaattagct gctcaaaaag acgaaataga ggaaaagaaa cgatggattg
    37201 ggtattatga atttacgcaa cgattagtcg aaccaatcca gcgcctacaa gaaataaaag
    37261 ttgaaaaaat agaactgtca caaaaaagta gtgagcaaac aaagaaacta gcgatgacta
    37321 cgcagaatta tgaagagtgg caaaaaaatg aagcaaaccg cgtacgccga caaacaaaaa
    37381 tcaaagaagc tgctcaaaaa ctgcaaaaca aacagatgat ggtgccaatt gtcgaatcct
    37441 tacgtagtaa aaaacaagac gcagaacaat taacaatttc attggataaa gaagaattga
    37501 atgccgagaa gctgtatcaa aatctcgcta tctatcagga aagaactcga attcttcaag
    37561 aaaatttact ggatcaggaa aaactgcaag aagatcgtct tgcttttgga cagttaaaat
    37621 atctagatga aaagcagcaa gaaatagaaa aacaaaaagc aaaagacact gggtcgctcg
    37681 cacagataag agaagaactc aaaaaagcaa ctgaagaaat aacaaaaaat agtcagctat
    37741 tgaaagaaca gacagaacaa gcagaacagc tgaaaagcca atgggcaaag cagcaaattg
    37801 ctcgtcttca gctttttttg cgtccagggg agccatgtct tgtttgtgga tcaacggaac
    37861 accccataca acaattaaaa cacgaacagc caaataagga agatatctta aaagtagaac
    37921 aacaactggc agaagctgag tctgctgtag aaagaactca gcaaatcatt gccaaaagtg
    37981 aagcacatga aaataatctt caacaacaat tatcagattt agaacaaaaa atcatggcaa
    38041 cgaatataca attggctgaa caagaagaac acttgcaaca ggctttgaaa aaggcatttc
    38101 ctgagataac agaacaaaaa acaaaaacaa ctcttgtagt aatcgaacaa tctttagata
    38161 gacgaaaaag caagatggcc aatgcaagaa tagagctgga agaaataaga caacaagcag
    38221 aaaagctcga acaacaatta gctgtctaca aacagcagca gacagaaaaa aataatcagc
    38281 tccagcaact gcaaggagaa atcaaaagtt tagaacaaca attgcaagat acggatagta
    38341 actgtttaga agaggagata cgacgacttg aaaaacagtt agaagaagac tcgaatcaac
    38401 tagctgttga gcagaaagca ggagtgcaat tgttgcaaga tcttcatacg cttcggacac
    38461 agcttgatct tcaacatgaa caaatagaaa aaatagatga aaaaatagaa aaggaacaag
    38521 aaagaatagc taaaaagtta gaaagccagt cgtatttttc aacaattgaa gaaatctgtg
    38581 catttgcaca acagcaagca caatataaag aaaaccaacg tacagttact tcttatcatg
    38641 aagagcaatt agtgacagct gaccggctgc gtcaattaga aaatatccag ctaactgaga
    38701 cattcccaga tacagaagag atcagccaac agttagaaga aataagacgt atgatcgaaa
    38761 taaagcaaga aaagatatac ggtttacaag aacaaaaaat tagtaatcaa aaaatctacg
    38821 atgaatgtct ttcgatttat cacacaagcc agacgaaatt agatgagctt acgcaattac
    38881 agcagctttc gcaaacattc aacggagaaa atcctaagaa gactagtttg gaacggtatg
    38941 tgcttcaggt ttatcttcaa gaagtgttac aagtagcaaa tgatcatctc caacgtttaa
    39001 cgaaaaatcg ttatcaattt gagctagcgg ataccattgg cagttatcga gggaaaaccg
    39061 gattagaaat caatatctat gatgatcagg caggtatgtc tcgaagtgcg cataccctgt
    39121 ctggcggtga gagttttatt gcagcacttt ctttagcatt agctcttgcc gatgtgatcc
    39181 agacacaagc tggtggtgta gcaatcgatg cattgtttat tgatgaagga tttgggtcat
    39241 tagatgaaga agctttggaa atggcaatgg aagctttaga aacgatcgaa agtgaaggaa
    39301 gaatgatcgg catcattagt catgtaagag aattgaaaga acgagtatta caacaaataa
    39361 gaattgatac agaaggtagc ggacaaagca aaatacgcta tcaacttgga taaaaaggga
    39421 gattggtaac tatgaaatgg agtattccag aaaaagtcat tgcacgtgga cgtcaatact
    39481 tgcaagaagg ccgagtgtta tcggttgtcc ctgaccaaga agaaaacgtg tggcatgcag
    39541 aagtcttggg cagtgaattg tatatggttg atttggatgg tacagcaaaa gagatagatt
    39601 actgccaatg tccctattgg gaggaacata aatattgtaa gcatacagtt gctgtggaac
    39661 tttatttacg gtcgaaaaat ctatctcgta tcatgaaaaa agaccagcca atgctagaac
    39721 cggtaaaagc aacaagtgag ggagaaatca ttgcaaaagg tttttctcgt ctaaaaacac
    39781 aaaacaatcc tcaagcggtg caacctttga ttattgaata ccaagtagaa acgattgaga
    39841 ctaatcagta tcacccagag ctttctattt tggcacttta cttgcgtatc ggttcattgg
    39901 catcaccgaa aaaaacatat atcgtaaaaa acatctatga ttttttacaa gcttacacag
    39961 aaaaagaaac ttacactgtt aataaacagt accagtttcg tctagataaa cgggcgtttc
    40021 aaaaagaaga tcaaaaaata ctcacgcatc ttgcaggaag tgctcagacg catcaattat
    40081 taggtacgaa cggcttacaa gtcaaaggaa aactagataa aagatatctg cttctcccag
    40141 tagaacaagc ccgcttcctg ttagagcaga tgaatcagac tacacgtttg catctgcaga
    40201 taaatgaaca aaacgatcaa ggaatacaat tttccgatca aaacaaacct ttatcattca
    40261 aagtggaaaa atttggagaa gattatttgc tgcaagcatt gaatgatttt gattttttct
    40321 ttatgcatta tcagtgggga gttatccaag ggacatttta ttctctgacg aaatatcaac
    40381 aaacggttta tcaaacttgg aaacaattga ttcgccgatt ggagaaacca gaagtagtgt
    40441 tcaagaaaaa agaattaccg tctttgttta aagagattat ccctctgctg tcagagatcg
    40501 gcgacgtagc tattgcccct gcagtagaag aggaagtagc agatatcccc gtcgaattta
    40561 ttttcttttt ccgaaaagca aaaggaaaaa tccaagctcg agtcgatttc gtctatggcg
    40621 aagtcatcta ttcgactgac ccgaagcatg aggtctcgac agaacaggct tttcaggtgt
    40681 tacgtgacac agtagaagag aaacgagtac ttgatctatt cagaatgtat cgctaccaga
    40741 aaaatgaaac tggttatgaa cgtccattgc caagcgggga agaactatat gcctttttcc
    40801 gcacagaact atctgtcttc cgtcagtttg gtgaagtcag acttggaaga aaattgcgag
    40861 agttgtattt agatgcgcat aaacatcaac cgaaaatcga agtaaccgag accggttcat
    40921 ggctcgatat tcgattcgat gtgacaggga tagaggagca agaaatcgat catgtcctgc
    40981 aaagcttgct gagaaatgat gctttttata cattagaaaa tggtcaggtt ctttcttttg
    41041 attcagaaga atttcaacaa acaagccaag tgttgcaaca attacgggaa tcgatacgta
    41101 cagaagaagg aacgatccat gtacctaaaa atcaaggatt gatcattcaa aatcaattgg
    41161 aaaaaagcaa tgctactttt agtgaatcgt tccaaacgat ggtacaagat ctcattcatc
    41221 cagaaagata ccaggcacag cttcccaaag gattgaatgc aacgatgaga gattatcaaa
    41281 agcaaggttt tcgatggttg aagatgttag gtcattatca gtttggcggt attttagccg
    41341 atgaaatggg acttgggaaa acactacaga caattgcctt tttactttcg gaaaaagaag
    41401 aacggaaaag cttctctgct ttgatcgtag cgcctgcgag cttaatttac aactggcagg
    41461 cagaggtgag aaaattcgcc ccttctttaa gtatccaagt aatcaatggg aataaaaaag
    41521 aacgtgaaga attactggct aaagacacag atatccgagt gacttcttat gccagcttaa
    41581 gacaggatct agctaattat cagtcgcaaa aaattgatta tttgattctt gatgaggcac
    41641 aaatggtcaa aaatagcagt acgaaaactg cacaagcatt gagagaatta gctgtacctc
    41701 agcggtttgc actgagtggg acaccaatcg agaataattt agaagaattg tggtcattgt
    41761 ttgcaacgat tatgccagga tttttcccga ctaaaacaaa attcagggaa cttgccgcag
    41821 aagaaattgc gcaaatgatt cgaccgttta ttttacggcg agataaacaa actgtgttga
    41881 aagacttgcc ggaaaaaaca gagatgaatc tatattctgc acttacagaa gaacaaaaaa
    41941 ctgtttattt agcttacttg cgtcaaatgc gtgaagaaat cacatcaatg gattctgaag
    42001 cattcaagaa aaaccggatc gggatacttg ctggtctgac aagactgcga caaatttgct
    42061 gcgatcctcg tctgtttatt gaagattacc aaggcggttc cggcaagctc gaacaagtaa
    42121 aagatctact ggtagctgcc aaagagaaca aacgaagagt tttgctgttt tctcaattta
    42181 cagggatgct gactatcttg caagaagagt tagccgagct agggatctcg acattttatt
    42241 taagaggcag tacgaaacca caagatcggt taagtatggt agacgcattt aatgctggcg
    42301 aaaaagatgt ttttctgatt tccttaaagg ctgggggaac tggattgaat ctcacgggag
    42361 cagatacggt catcttgtat gacctttggt ggaatcctgc cattgaagaa caagctgctg
    42421 gacgtgcaca tcggatcgga caaaaaaatg tggtagaagt gtggcgtatg atctctgaag
    42481 gaacgattga ggaacgcatg gattcactcc agcaagaaaa acgcgaactg ttccaaaaag
    42541 tgatccaagg taatgaagaa cagttgacga aactgactga agaagacatc cgcatgattt
    42601 taagtgtagg agaaatcgag gagtaagaga ctcgaaaaag cgttcagtaa tgggtaagaa
    42661 agcctttcta aagaagcgat tgtttatttg atttaaaaaa tagcaaggag gctgggattc
    42721 cattttatcc cagcttcctt gctatcattt ttttatttct atttttaaag gttatacaaa
    42781 gattgtcttc attgtctcta acagctgatc aaaagaagaa atctcgatat cgcaaattgg
    42841 agtctgttca attttgcgtt ctcgatggtt gaaccaaagc gcttgccagt tagcactttt
    42901 tgcacctaga acatcattgt cgtaattatc gccaacgtat aaggtctctt ctggaagcat
    42961 atgaaattct ttctcagcta gctgaaaaat ctctttttct ggtttttgga aacctgttgc
    43021 ttgagaaatg agcatattgc gggaaggaat ccaattgttc aattgaagtt gcttcagttt
    43081 ttttgtctga tggtcagtcg gcccatttgt gataattcct aatgggacat tcttttcctt
    43141 caagaaatcg agggtttttt tgacctcctc atgcaagcag atattatcaa gttcttcctc
    43201 gtaaattttt tgaaataaga ggccatcttc ttccgtaata tgagggtagt ctaaatcttt
    43261 tagtgattgg ctgatccgat gtgctcgcat atattctagt gtccaatctc ctgccattac
    43321 ttttgggaag ttttcatcac tatgatggcg gaaccggata tatagtggat gcatatctgc
    43381 atctgaaaca agcggaacga ctcttttgac cgcgtttcga aatggctgct gctgatcata
    43441 catcgtatca tcaacatcaa acacaactga tttaatcaaa attttacacg cctttcggtt
    43501 ttcattgatt ttttcacaaa tccttgacta ttgtaacaga aaaaattgtc taaaaaagct
    43561 gataaataaa ggtttttctt actatatttc tatttccttg acagaataag gtttttatat
    43621 ttcagttttt ttcactaaaa atcgtgaaac acgtcaataa gtttgatgaa caaattaatc
    43681 attatggtag aagtcgattg atttatttta atgcaatctc tgtggaattt tcgaaagatt
    43741 tattttt
//
LOCUS       DACCXW010000008        43378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCXW010000008 DACCXW010000000
VERSION     DACCXW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.008"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            37..2634
                     /gene="adhE"
                     /locus_tag="IVY71_000354"
                     /gene_synonym="adhC"
     CDS             37..2634
                     /gene="adhE"
                     /locus_tag="IVY71_000354"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7984429.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2863..3969
                     /locus_tag="IVY71_000355"
     CDS             2863..3969
                     /locus_tag="IVY71_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7984430.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3986..5014
                     /locus_tag="IVY71_000356"
     CDS             3986..5014
                     /locus_tag="IVY71_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP7984431.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5020..5499
                     /locus_tag="IVY71_000357"
     CDS             5020..5499
                     /locus_tag="IVY71_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7984432.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5515..6810
                     /locus_tag="IVY71_000358"
     CDS             5515..6810
                     /locus_tag="IVY71_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP7984433.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6821..7645
                     /locus_tag="IVY71_000359"
     CDS             6821..7645
                     /locus_tag="IVY71_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7984434.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7810..8709
                     /locus_tag="IVY71_000360"
     CDS             7810..8709
                     /locus_tag="IVY71_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7984435.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8835..9704)
                     /locus_tag="IVY71_000361"
     CDS             complement(8835..9704)
                     /locus_tag="IVY71_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7984436.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9857..11317
                     /gene="rhaB"
                     /locus_tag="IVY71_000362"
     CDS             9857..11317
                     /gene="rhaB"
                     /locus_tag="IVY71_000362"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP7984437.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11314..12570
                     /gene="rhaA"
                     /locus_tag="IVY71_000363"
     CDS             11314..12570
                     /gene="rhaA"
                     /locus_tag="IVY71_000363"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP7984438.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12584..13429
                     /gene="rhaD"
                     /locus_tag="IVY71_000364"
     CDS             12584..13429
                     /gene="rhaD"
                     /locus_tag="IVY71_000364"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP7984439.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13431..13742
                     /gene="rhaM"
                     /locus_tag="IVY71_000365"
     CDS             13431..13742
                     /gene="rhaM"
                     /locus_tag="IVY71_000365"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP7984440.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13824..14975
                     /gene="fucO"
                     /locus_tag="IVY71_000366"
     CDS             13824..14975
                     /gene="fucO"
                     /locus_tag="IVY71_000366"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP7984441.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15102..15860
                     /locus_tag="IVY71_000367"
     CDS             15102..15860
                     /locus_tag="IVY71_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP7984442.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15960..16604
                     /locus_tag="IVY71_000368"
     CDS             15960..16604
                     /locus_tag="IVY71_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7984443.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16611..17618
                     /locus_tag="IVY71_000369"
     CDS             16611..17618
                     /locus_tag="IVY71_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP7984444.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17634..18476
                     /gene="kduI"
                     /locus_tag="IVY71_000370"
     CDS             17634..18476
                     /gene="kduI"
                     /locus_tag="IVY71_000370"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP7984445.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18496..19299
                     /locus_tag="IVY71_000371"
     CDS             18496..19299
                     /locus_tag="IVY71_000371"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7984446.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19548..20612)
                     /locus_tag="IVY71_000372"
     CDS             complement(19548..20612)
                     /locus_tag="IVY71_000372"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7984447.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20609..21694)
                     /locus_tag="IVY71_000373"
     CDS             complement(20609..21694)
                     /locus_tag="IVY71_000373"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7984448.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21707..22684)
                     /gene="mvaD"
                     /locus_tag="IVY71_000374"
     CDS             complement(21707..22684)
                     /gene="mvaD"
                     /locus_tag="IVY71_000374"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7984449.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22677..23621)
                     /gene="mvk"
                     /locus_tag="IVY71_000375"
     CDS             complement(22677..23621)
                     /gene="mvk"
                     /locus_tag="IVY71_000375"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7984450.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23943..24596
                     /locus_tag="IVY71_000376"
     CDS             23943..24596
                     /locus_tag="IVY71_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7984451.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            24681..25586
                     /locus_tag="IVY71_000377"
     CDS             24681..25586
                     /locus_tag="IVY71_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7984452.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25588..26220)
                     /locus_tag="IVY71_000378"
     CDS             complement(25588..26220)
                     /locus_tag="IVY71_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7984453.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            26540..27064
                     /gene="infC"
                     /locus_tag="IVY71_000379"
     CDS             26540..27064
                     /gene="infC"
                     /locus_tag="IVY71_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7984454.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27136..27336
                     /gene="rpmI"
                     /locus_tag="IVY71_000380"
     CDS             27136..27336
                     /gene="rpmI"
                     /locus_tag="IVY71_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7984455.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27389..27748
                     /gene="rplT"
                     /locus_tag="IVY71_000381"
     CDS             27389..27748
                     /gene="rplT"
                     /locus_tag="IVY71_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7984456.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28000..29337
                     /locus_tag="IVY71_000382"
     CDS             28000..29337
                     /locus_tag="IVY71_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7984457.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29385..31514
                     /locus_tag="IVY71_000383"
     CDS             29385..31514
                     /locus_tag="IVY71_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7984458.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31489..32178
                     /locus_tag="IVY71_000384"
     CDS             31489..32178
                     /locus_tag="IVY71_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984459.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32586..33263)
                     /locus_tag="IVY71_000385"
     CDS             complement(32586..33263)
                     /locus_tag="IVY71_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7984460.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(33399..33593)
                     /locus_tag="IVY71_000386"
     CDS             complement(33399..33593)
                     /locus_tag="IVY71_000386"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984461.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33643..34083)
                     /locus_tag="IVY71_000387"
     CDS             complement(33643..34083)
                     /locus_tag="IVY71_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7984462.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34087..34932)
                     /locus_tag="IVY71_000388"
     CDS             complement(34087..34932)
                     /locus_tag="IVY71_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7984463.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35081..36499
                     /gene="pepV"
                     /locus_tag="IVY71_000389"
     CDS             35081..36499
                     /gene="pepV"
                     /locus_tag="IVY71_000389"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7984464.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36556..37503)
                     /locus_tag="IVY71_000390"
     CDS             complement(36556..37503)
                     /locus_tag="IVY71_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7984465.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37726..38076)
                     /locus_tag="IVY71_000391"
     CDS             complement(37726..38076)
                     /locus_tag="IVY71_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7984466.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38276..39355
                     /gene="pepA"
                     /locus_tag="IVY71_000392"
     CDS             38276..39355
                     /gene="pepA"
                     /locus_tag="IVY71_000392"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7984467.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39499..39819
                     /locus_tag="IVY71_000393"
     CDS             39499..39819
                     /locus_tag="IVY71_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7984468.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39841..40305
                     /locus_tag="IVY71_000394"
     CDS             39841..40305
                     /locus_tag="IVY71_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7984469.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40510..41115
                     /locus_tag="IVY71_000395"
     CDS             40510..41115
                     /locus_tag="IVY71_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7984470.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41154..42443)
                     /locus_tag="IVY71_000396"
     CDS             complement(41154..42443)
                     /locus_tag="IVY71_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7984471.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42871..43350
                     /gene="rlmH"
                     /locus_tag="IVY71_000397"
     CDS             42871..43350
                     /gene="rlmH"
                     /locus_tag="IVY71_000397"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7984472.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        13697 a         7935 c         9301 g        12445 t
ORIGIN      
        1 tttaacaaat aaccatgacc cattaggagg ataatgatgg ctaaagtaat agaaaaagaa
       61 aaaacgaaga caattgacgt acaagcaatg attgatgatt tggcagaaaa agcaaatgca
      121 gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag accatatcgt gcatgaaatg
      181 gcaatggcag ctttggatca acatatgcca ttagcgaaga tggctgtaga ggaaacaggc
      241 cgcggtgttt acgaagataa agcaatcaaa aatatgtatg cgtccgaata tatttggcat
      301 aatatcaaac atgataaaac agttggtgtg atcaatgaag atgtccaaaa agggttgatt
      361 gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc caacaacgaa tccgacttcc
      421 acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa atccaatcgt ttttgctttc
      481 catccaagtg cccaaaaatc ttctgcagaa gcagcacgag tggtacgtga tgctgctatc
      541 gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac atccttcgat cgaagcgact
      601 tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag cgacaggtgg agctggaatg
      661 gtcaagtctg cttactctac aggaaaacca gctttaggtg taggtccagg taacgtacca
      721 gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa
      781 acatttgata atggaatgat ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc
      841 tatgcagcag taaaagcaga attccaagca caccaagttt atatcgtaaa accagatgaa
      901 ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc
      961 gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg
     1021 aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt atccgctatc aagagaaaaa
     1081 ctttctccag ttcttgccat gatcaaagct aacaacacgg accatgcgtt tgatttgtgt
     1141 gaaggtatgt tgaatcttgg aggattagga catacagcag ttatccattc agaaaacgaa
     1201 gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc gtattttagt gaatacacca
     1261 tctgcagaag gcggcatcgg tgatatctat aacgaaatga ttccgtcatt gactttggga
     1321 tgcggttctt atggtaagaa ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat
     1381 gtcaaaactg tagcgaaacg gagaaataac atgcaatggt tcaaattacc gccaaaaatc
     1441 ttctttgaaa agaactctct attgtactta gagaaaatgg aaaacgttga acgtgtcatg
     1501 atcgtttgtg accctggtat ggtccaattc ggttactgcg atacagttcg tgaagtattg
     1561 gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag atgttgaacc gaacccatcg
     1621 acaaataccg tatacgcagg aacgaaactg atggcagact tcaagccaga tacagtaatc
     1681 gctcttggag gcggttctgc aatggacgca gctaagggaa tgtggatgtt ctatgaacac
     1741 ccagacacat ctttctttgg tgcaaaacaa aaattcttgg atatccgtaa acggacatat
     1801 aagatcgaca aaccagaaaa aacacaattc gtatgtatcc cgacaacatc agggactggt
     1861 tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa cacatgtgaa atatccgtta
     1921 gcagattatg cattgacgcc agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt
     1981 ccagcatctg tcacagcaga taccgggatg gatgtgttaa cacatgcaat cgaatcctat
     2041 gtttcagtca tggcttcaga ttatacacgt ggattgagcc tacaagcaat caagttagtc
     2101 tttgactatt tagaaaaatc agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac
     2161 aacgcttcta caatggctgg tatggcgttt gctaacgcat tcctaggtat ttgtcactca
     2221 gtcgcacata aaatcggcgg ggaatacggg atccctcatg gacggacaaa tgcgattctt
     2281 ttaccgcata ttatccgcta taatgcaaaa gatccacaaa aacatgcgat gttccctaaa
     2341 tatgactatt tccgtgcaga tacagattac gcagatatcg caaaattctt aggtctgaaa
     2401 ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg ctgtttatga attaggaaaa
     2461 tctgtcggca tcaacatgag tctaaaagct caaggagtca cacaagaaac actagataca
     2521 accgtagacc gtatggctga attagcttac gaagaccaat gtacgacagc taatccgaaa
     2581 gaaccattga tctctgaatt gaaacagatc atcattgatg cttacaacgg ataagcaaaa
     2641 gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa cttggaaaac gttgtttctc
     2701 caagttcttt ttttaatttc caagtaactg aaagttactt ttgttattta acatttaggt
     2761 caattttaga cagtttttgt tcaaaaagcg atatttatag tggacggtat tactgctata
     2821 ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa agatgattat taaagaagag
     2881 atttttgtac ctaaagaatt agtgaaagaa aaaattgacc tgctcgttga aaacctgaca
     2941 acgatcaaag acgataatgg tgaatttttg ttggactttg atggactgaa agtagatgac
     3001 aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc tatatggtat ttataaaaat
     3061 taccagatga caaaaaatcc tcgcgcttat caagtggtca atgaatggtt tgaggatcgt
     3121 atggaagaag gcgctccgcc caaaaacgtc aatacaatgg caccgctatt gactatggct
     3181 tacttgtatg aagacacgaa agattcacgc tatttgcctt atttagaaca atgggcagaa
     3241 tgggtcatga atgacatgcc tcggacaaat gaagacgggt tacagcatgc cacatatgga
     3301 ccggaaaata agaatcagtt gtgggacgat acattgatga tgacagtgct tcctttggca
     3361 aaaatcggga aactgttgaa tcgcccagaa tatttagaag aagctcgtta ccagtttatg
     3421 atccatatca aatacttgat ggataaacga acaggattgt ggtatcacgg ctggacgttt
     3481 gaaggaaatc ataactatgc cgaagcattt tgggctcgtg gaaactgctg gttgacgatt
     3541 gctattccag agatcattga aatcttagaa ttatcgaaag aagatgcatt gcgcaaatta
     3601 ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa cttatcagtc tgaaagcggt
     3661 ttatggcata cgttgttgga tgatccttct tcttacgtag aatcttctgc aactgcagga
     3721 tttgcttacg gtattttgaa agcggtccat aaacgttatt taccacaaga atataaagaa
     3781 gtcgcttatc gtgctatcca aggattgctt gaacaaatcg atgaaaaagg agaagttcaa
     3841 aacgtttcaa tcgggacagg aatgggggac agtttagatt tttatcggaa tatcggaatc
     3901 acagcaatgc cttatggaca gtcgttaacg gtgctttgtt tgacagaatt gctcgtctct
     3961 tattgttaaa agaaaggaaa cccatatgaa aaaaacatca ctgctgtttg cagcaagttc
     4021 gcttagccta ggtctgctag gtctaagcgg ctgcggaaat aattctagtg tggcaactgt
     4081 cgcagatgca gatgtaaatg agatagttac cttgcgcttt gcttatgcaa gcaacagcca
     4141 gccagtaatt gatgcaatga atgaatttgg gcgtttagta aaagaaaaaa cagatggttc
     4201 cgtacagatc cagtatttcc cagatggaca actgggtgga gaacgtgaac tgatcgaatt
     4261 gacacagtca ggagcagtag atttcacaaa agtaagtgcc tcagcgttgg aaagtttctc
     4321 taaagattat tcgatttttt cagttcctta tctattcaat gatgaagaac atttctttaa
     4381 ggtgatggat aatgaagaaa taatggagcc aatctaccaa tcgaccaaaa atttaggatt
     4441 taccggattg acctattatg attctggtca gcgcagcttt tatatggtag acggtccgat
     4501 ccatacgccg gaagatttga agggaaagaa gatccgtgtg atgcaaagcg aaaccgctat
     4561 caaaatggta gagcttttag gcggttcgcc cgtacctatg ggaagtgatg aagtgtacac
     4621 gtctcttcaa tccaatttga tcaacggatc agaaaacaat gaatttgtct tgtatacagc
     4681 tggacatggt ggtgtggcta aatactattc ctatgatgag catacacgtg tgccagatat
     4741 catcatcatg aatgatgcca tcaaagaaca gctaactgat gagcaggaaa aagccattga
     4801 agaagcagcg aaagaatcaa cggtttttga aattgaagct tttgcaaaag caattgaaga
     4861 agaaaaagct ttagcacaag aagaacatgg agtccaattc aatgatgtag ataatacacc
     4921 gtttcgagag gcagttgccc cacttcatga tatattcaaa aacgatccta cctacaaaga
     4981 cctttatcaa aagatccaag agcaaggagc gtagctgtga tgaaaatgat tttaaacaag
     5041 gtattagaga tcctgggagc tgttttgctt gttgggatgg tcgctgttgt tttatggcaa
     5101 gtattttcca gaacggtttt aggaaatcca aatacggtca cggaagagct tgttcgattc
     5161 ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg tgggacaaaa agcacattta
     5221 gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa gtaaaatttt agaagttgtt
     5281 gttcaaagtt tatttctggt atttgctgcg atgatcatgg tgtatggcgg atggaatgct
     5341 gtcacattga caatggggca gatctcgcct tctttaagta ttcctatggg atatgtttat
     5401 ctgtcagtac ctgtatcggg ggtactcatc attatctata gcctgatcaa tctaatcgat
     5461 acattaagca acaagccagc aattcaagaa aaaatataaa aatggaggaa taaaatggca
     5521 atagaagccg gattagtgtt attttttgta tttgttgtgt tgcttgtatt agggatgccg
     5581 attgctataa gtattgcatt ctcatcgatg atgactttat tgttagttat accgtttgat
     5641 gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta tcaatagttt ttcgctgatc
     5701 gctattccgt ttttcgttct ctcgggcatt atcatgaata atggagggat agctaagaaa
     5761 ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg gggctttggc tcatacgaat
     5821 gtgttaggga atgctttatt tggttccatc tctagctcgg ccattgccgc ttctaccgcg
     5881 attggcggag tgttgatccc tcagcaagtg gaagaaggat atgaccggaa gtttgcgaca
     5941 gcggtcaata tcgcgtcagc tccgacaggt atggtcattc ctccaagtac tgcttttatc
     6001 atgtattcac ttgttgctgg tggtgcttcg atctctgctt tgtttatggg aggttatctc
     6061 gtaggcgctt tgtgggcgct aggaatcatg ctagtagcat tcatcgttgc taaaaagaat
     6121 aaataccctg ttgtcgaaaa aggacagatg gctcatgctg gaaaaataat aaaagaggcg
     6181 attcctagcc ttctattgat tgtcatcatt atcggtggta tcttgacggg gatatttaca
     6241 gcagtagaag cttcagcgat agctgtcatt tactccctgt tgattgctat gttttactat
     6301 aaaacggtaa aactaaaaga tatacctaat atgttgtttc aatcagtagc aatgtcaggg
     6361 acgatcatgt tcttattggc tacttcttcc atgatgtctt tcgccatggc gtttaccggt
     6421 attcctcagg caattagttc gttgattatg ggcgtaacgg ataataaatt tattattctg
     6481 ttattagtca atcttgtgtt attactggtg ggtatgttta tggatgtggc gcctgctatc
     6541 ttgattttta caccaatatt tttaccaatc gtcacaagtg taggggttga tcctgtacat
     6601 tacgggttgt tttcaatcat gaatctgtgt gtaggctcca tcacgccgcc agtaggtaca
     6661 ggactatatg tcggtgcaag tgtaggaggc gtaaaagcag aggcaatgct gaaaccgctt
     6721 cttccttttt atggggttat cttagtcgtc ttgtttttga ttacttactt cccgcaaatc
     6781 gtcatgtggc tacccaatat gctgagttag gagaatagtt atgttgaaca ttacaattag
     6841 aggacatgat ctttccgaag tccaaacgat agaagatctt gcagaaaaaa caaaagaaca
     6901 agggattcat acactgcagc tcgccttagg tctttcgttt cctgaaatac cttctggcgc
     6961 aaatgagatc aattctggga tgggaaatta tgtcaaacga gttttagaaa agcaagaagt
     7021 gaccgtagga attttaagtt gctatatcaa tatgattcat ccagatttaa cgatacgaca
     7081 agagttgctg acaaagttcg agcagtatct tcgctatgct tcttcctttg gtgcgtcgat
     7141 ggttgcttcg gaaactggat gtgttttgcc ggaaatccaa tatacggaag aaaatttcac
     7201 agatgaagca tttgctgaag ctgtgtccgt tatccgtcgc ttggtaaaag ctggagaaaa
     7261 ataccaaatg atggtgggga tagaaccagg tttgaatcat ccggtgtatt ctttagcaag
     7321 ggtagagcag ctgatccaag cagtagattc ggactatcta ggaatcattt tagatccgac
     7381 caatttgatc acaagtagtg attatcaaga acaagtccaa ttggtagaag aagcttttga
     7441 acgttttggt gaaaagatat gtgcggttca tttgaaagat tttcgtgtcg aacaggaaaa
     7501 aatcgtgccg gtaaatttgg gagacggtgt gatcgagtac acaaaaatca aagaaatcat
     7561 catgaagaat cgtccgtacc tgtatgtggt tcttgaagaa acaaaagatg atgggattcg
     7621 ttatggaaga agtcttcttg aataatgtaa agaagacgtt ggttaactaa tatttagaat
     7681 ggagggggcg gggctagaag atgccacgtc tcttcttttt tataagagta tataaaagca
     7741 gctctttttg attacaaaaa acgtggtata ataaaattgt gcataagtga acaaaaggag
     7801 gtgcggcaca tgctccaaaa gaaaatcagc ggtgaacaag tcaatacgat ggtttctgat
     7861 cagtatgaaa tctatcatgt caaagatgtc gtcagccaaa ataaaacgat ctatcatcat
     7921 catgatttct atgagatcca tgctacatta aagggagaag cgacttttta tttgaacgga
     7981 cgacaattta cgatcaaagc aggaagtgtg ctgctcatcc attccaatga tttgcatcgt
     8041 atcgtccgcc aatcttcaga tgtattcgag cgtgtctata tctttgtcac cccttctttt
     8101 ctagaaagtc gttccacgcc atggtctaat ctatcggctt gttttcaacc aatgggagag
     8161 aagaggagtc gtgtattgca aatagaacag gaagtattga agcaaaaatt atcatttatc
     8221 gaccatcctc ctgataaaaa aatttacggt gaagacataa aatatgaaca agctctggta
     8281 gaatacttga tttttctcaa tcaagttgta caaaaagaag agagtgcgca agaacagaat
     8341 gctgtagttc ctaatgagcg tgtggaacaa atgttacaat atatctcaaa aaatttatct
     8401 catccgcttt ctttagagga aatggaaaag cagttttttg tcagcaaata ccatatcaca
     8461 cgagaattta aaaagtacac tggatttacg tttcatcagt atgtaatgaa gaaaaagctg
     8521 atctatgcca agcacttgtt gcgagaatac cgcagctcta gtgctgtata tagtaaaagt
     8581 ggtttttctt cttatccgca ttttctgaaa gcattcaaga gagagttcgg cataacgcct
     8641 aaagaatttt tgaaacgtga tacacagaaa cagttggttc attatgaaca ttttgaagat
     8701 gaacaatgag aatgatcact aaaaaataac cgcagtagct gctgttgttt acatagaagc
     8761 agaaactgcg gttattttat ttttatctat atatttcaaa atattttgaa aaaatgacgt
     8821 acatattata tgaatcagct gaatttacga aagtctctcg gcgatagtcc tgtttttgct
     8881 ttgaacaggc ggctaaagta agcaaaattc tcaatcccta tttcttcgga aatcattcga
     8941 accgtaaatt ctgtataccg aagaaggtct ttagcgtaag aaagtttttt gctgatcaaa
     9001 taatcgattg gtggagaacc gataaatttg gaaaaatctt tgctcagctg atatttattc
     9061 aataagaatt gttgttctaa ttgatcaagt gtaatcgttt ctttgaaatg ttcgtctaag
     9121 tattgcttca gtttgtaaac gtaggcggga atatcctctt cttcgaaatc tagctgatac
     9181 ttttgcaaaa tcagttcatt cagcaactcg tggatagcta cagaggactg gaaatcggta
     9241 cgggcattcg tgccttgttg catgtggatc aattctgtga tcaaatggtg gatcttgttt
     9301 tctgatggat ctcctgttga atggaaggtg ttatggccgt cttttgtgaa ctcctgataa
     9361 aatgattgga cgttctgacc attcaaatgg atccaatcca tttcccatgg ttcttcgctg
     9421 atcgtttgat aacgctgata ttcttggcag tcaataaaaa ataagtctcc agcttttagc
     9481 tgttgctgat tatcattata tgtaagcgaa cctttgccag acaaagtaaa ctttatcaag
     9541 taagaaggta aatgtgctcg ttcagtatag taagggattg gtgctttgaa gtaaccgatt
     9601 tcttggatat aaaaaaataa attcctgctt ctttgtgtag gcgtcaagat cactcttttt
     9661 gagtcttccg accatgagtg gttggctttt tcccagtaat ccatgtaaac actcccaact
     9721 tgtcaagata atgcaattat ataccaaaat agtgtgaagc aaaatataag attctgtatt
     9781 atgatagcat tgtcaataaa tgataacgct ttattaataa agtgctaatc aacaataaag
     9841 taaaagaggt ggagaaatga atccaacatt tgttgccatc gatatcggtg catcaagcgg
     9901 acgactaatg aaaagtacga gggatgcaaa aggttctttg atcttggaag aagtacaccg
     9961 gttcaaaaac ggctttcgga acaaagacgg tcacgatcgc tggaagatcg attatcttat
    10021 caaagagatt ttgattggat ttgaaaaatt aaagcagaca gggattgatc gcctttttgc
    10081 ggggatcgat acatgggcag tcgattactg tctgattgat gaacaagggg atcggataaa
    10141 tgatccgatt gcttatcggg acaagcgtac caatggagca gtagaacagt tttctgaaat
    10201 ctattctcta gataagttgt atgagcagac agggattcag atccagcctt tcaatacctt
    10261 gttccaattg tttgctgaag aaaaagaaaa tttggcaaaa gcacataaat tgttactgat
    10321 acctgattat ttagggtatg tctttactgg taacatggtg acggagaaaa ctaatgcatc
    10381 tactatgcag ctgctgaatg cagggacaaa agaatgggaa atagaacttc ttgaaaaaat
    10441 cggtgtctcg aaggaattat ttccaccatt gacagagccg ggaacgatac taggagatct
    10501 gcaaaaagaa caattttcaa aatatgattt gccagatgtg acctttatca cagtagcaag
    10561 tcatgatacg gcgtcagctg tattaggaac gccaggtcaa ggagaacgtt ggggttatat
    10621 cagcagtggg acatggtcgc ttttagggat tgaaacaaag atcgccaatg tttctcgcaa
    10681 tgctttttct gaaaattata cgaatgaatg gggtgcccat aacacggtgc gttttttgaa
    10741 aaacatcatg gggatgtggt tgatccagga agttgcccga atgcaagagt acgagtacag
    10801 ttatgcggaa ttggcagagc ttgctgcaaa agaaaaaccg ctgcagcaag tgatggaaat
    10861 caatgaccct cgatttttga atcccccaaa tatgatagaa gagattcagc aatactgttt
    10921 tgaaacagag cagccagtcc ctgtctcatc gggagctatc gctcgttgtg tgtatgataa
    10981 tcttgctctt tgttacgcac aagagctgat caaagtagaa cacttaactg gaacgaaatt
    11041 ggaaaagctg cacatcgtag ggggcggatc gaataatcgt ctactgaatc aattgacagc
    11101 agattatgcc ggaatcattg tagaagcagg acctagtgaa gccacagcaa tcgggaatgt
    11161 tgccatgcaa atgctcagta caggtcagtt ttcgaatata gaacaagcaa gaaatgtaat
    11221 caaacagtcg tttccatgtg aaacttttta cccagatgaa aataagcaaa acacgatgga
    11281 atggtacgtg aataaaatca aaggagtaat cgtatgaaca cgattgaaag aaattacgaa
    11341 caagcaaaag aaaaatatgc aactatcggt gtagatacag atgctgtact agaaaagata
    11401 caggacatca aaatttctat gcattgctgg caaggagatg atgtcaaagg tttcttgaca
    11461 ccggatggcg aattgaccgg tggaatcatg gcgacaggga attttccagg agccgcacga
    11521 acacctgaag agcttagaca agatctggag aaggcttatt cgttgatacc gggcaaacac
    11581 aaattgaacc ttcatgctat ctatttggat acagaagaat ctgtcgatct aaatgaaatt
    11641 gagccgaaac attttgaaaa atgggtagag tgggcaaaaa aagaagaaat cggtcttgac
    11701 ttcaatccga ctttcttttc gcatccaatg atgaaagacg gctttacatt ggctcatcca
    11761 gataaagaag ttcgtgaata ctggatcgaa cacgggaaaa gaagccgcaa aatcgcggag
    11821 tactttggga aagaaactgg gcaaacaagt atcaataact tttgggtacc tgatgggatg
    11881 aaagataatc cgattgatcg ttttacccca cgggaacgtt taatggaatc actcgatgaa
    11941 atttttacag aagagttaga tgaacggtat gtacaagatg cagtagaaag caaactattt
    12001 ggtttaggcg ctgaagctta tacagtcggt tcccatgaat tttatatggg ttacgggttg
    12061 actcgtgaca aacttgtttg tctagatgct ggacattttc atccaacaga agtgatttcc
    12121 aataaattat cttcattagc tttattcagc aaaggaatca tgttgcatgt ttcccgtcca
    12181 gtgcgttggg attcagacca tgtggttttg atggatgatg aattgcagga aatcgctaaa
    12241 gaattagtcc gtaacgaatt gttggaaaaa acaaatatcg gtctggattt ctttgatgca
    12301 acgatcaacc gtatcgctgc ttgggtcatc ggtacgagaa atacacaaaa agcactgctg
    12361 aaagctatgc tggaaccagt tgagcgattg aaagaaatgg agctggcttt tgactttact
    12421 tcaagaatgg cttatacgga agaattgaaa gatttcccgt atgccgatgt atggaattat
    12481 ttctgttaca aaaatcaagt tccagttggt ttggattggc tggaagaagt ccaggaatat
    12541 caaaaagacg tgttggaatt aagaaaatag gagtgtggct atattgaaaa aaatagatat
    12601 tttacaagcg ccttatgtga aagaaatgat cgaaacgacg tcgaatcttt atcgcttggg
    12661 atgggatgaa agaaatggcg ggaatatttc ttatttacta aaagaagaag agatcacacc
    12721 gttcttggat cctgaaaaag tgatccgccg catctcgatg atttttgatg ccagccgttt
    12781 agcaggatgc tattttattg taactggttc aggaagatat tttaaaaatg tagcgagcga
    12841 acctgccgag aatcttggaa tcgttcgagt tgctgaagat ggcaaaacac tggaactttt
    12901 gtgggggctg gaaaacgaat gccttcctac aagcgagttg ccaagtcatt tcatgagtca
    12961 tatcgcccgt ttagaagtag acccagataa tcggatcgtt atgcattgcc atgctagtca
    13021 tctattagcg atgagtttta cgcatgaatt agacgaacgt tcctttagtc gtacgctatg
    13081 gcaaatgtgt acggaatgct tagttgtttt tccagaagga atttccatca ttccgtggat
    13141 ggttccggga acaaatgaga ttggggaagc aacagctgaa aaaatgaaag aaactcgatt
    13201 ggttttatgg ccacaacatg gaatctacgg gtcaggaaaa gacatggatg aagtctttgg
    13261 tttgatcgag acagcagaaa aggcagcaga agtctatacg tacgtcaaag cgcaaggacc
    13321 aatcttgcaa acaatcacgg acgaaaacct gtggcattta gcagatgcgt ttggtgtcac
    13381 tccaaaagca ggttatttgg aggaagttca tacgaaagca ggtgtctgac atggtgaaaa
    13441 aagctttttg catgaaagtt tacccagaaa aacaagaaga atacaaaaaa agacatgatg
    13501 aactttggcc agaaatgcgt acagcacttc gtgaacacgg ggtcatcgac tattcgatct
    13561 atcttgatcc gagtacaagt ttgttatttg gttacttagt gatcgaagat gaaaagttat
    13621 gggaaaaaat ggcaaataca tctatcaatc aaaaatggtg gaagtacatg gaagatatca
    13681 tggagacaaa tccagatgcc tctccagtca gttgggaatt gatgccagtc tttgaactgt
    13741 agcaacaagc aactgggcaa ccggttgctt ggggttcttt cgtttttatt gtgaaaaata
    13801 atacaaatta ggaggaatag aaaatgagta atcgaatgat tttaaatgaa acatcgtatt
    13861 ttggaaaagg agcgattgag tcgatcgtcc ctgaatttca gcagcgagga ttcggcaaag
    13921 cagctgtcat tactgacaaa ggattgatcg agcacggtat agcaacaaaa gtaacagaac
    13981 ttctagataa agcagcaatc gcttatgctt tatacgacgg gatcgtacct aatccaacga
    14041 tccaaaatgt aaaagatgga gtggcttttg ttaaagaggc agaggcagac tgtttgatcg
    14101 caatcggtgg aggttcgcca atcgatacgg caaaagccat tgggattatt ttgacgaatc
    14161 cagagttttc agacgtcatt agtttagaag gtgttgctga tacgaaaaat ccatgtttac
    14221 ctatattagc tataccaacg acttctggaa cagcagcaga agtaacgatc aactacgtca
    14281 tcacagatga agtaaatcac cgaaaatttg tttgtgttga tcctcatgat atcccaatcg
    14341 tcgcgttcat cgacagcgat atgatgatgg gaatgccgaa aaaattggct gcatcaacag
    14401 gtatggatgc tatgacgcat gctattgaag ggtttatcac aaaaggcgct tgggaaatga
    14461 ctgatatgct gcatttaaaa gcaatcgaaa tcatcggcca ttctttggaa gcttctgtag
    14521 atggcgatca aaatgggcga gaaaaaatgg cattaggaca gtatattgcg ggaatgggct
    14581 tctcaaatgt cggtttagga ctggtacacg ggatggctca cccgttaagt gcatggtaca
    14641 acattcctca tggcgtagcg tgtgcagcac ttttgccaac tgtcatgaaa tataacaaag
    14701 aatatactgg agaaaagtac cgtgagatcg ctcttgttct agggattaaa ggagcagctg
    14761 aaatgtcttt agaagatgta agagaagcgg cttgtggtga gatcgatcgg ttgagcaaag
    14821 cggttggaat cccagaaacc atttctgaat taggtgtaaa agaagcagat attccagcaa
    14881 ttgcagaaga tgctttgcga gatgtatgca caccaggaaa tccaagagaa acgacagtag
    14941 aagaaatcat tgctttatat caaagtttga tgtaaaaacg aagcggatct tcaacattaa
    15001 attgcctgta aagagaaatg gtagtttata tctggatatc tcttttaaat tgatggtatt
    15061 tccgctataa tttttcttat aaagaaatag gagaatatta tatggaaaat aaaaagcctt
    15121 atggaactgt attgatcaaa gcctctaaaa tactagacta tttagcggaa aaccaagatg
    15181 tctctttgca gacgatcgca aagggtgtgg gaatgacctc ttctacagtc ttgaaaatac
    15241 tagatacttt gttgatgatc ggctacgtaa ataaaaattc agagaaaaac tatcgtttag
    15301 gtgcaaagct tattcgctac gcgaataaaa acattgaaca aatcgatttg gtagagttga
    15361 ctttaccgtt tttagaacag ctgcagcaaa agatcgatga gactatccac ttaggggtat
    15421 tggacaacaa tgagatcttg tacgtgaaca aactagatcc aaaacatcaa acgatccgca
    15481 tgtcttcaaa aatcggaatc acccgaccat tatacaattc tgcgatgggc aaagccgtat
    15541 tagcagaatt cagtgaagag cagtacactg attatcttga aaagcatccg ttgatccctt
    15601 acacagaata tacgatcacc aatgcattac ggttgaaaaa ggaaatcaaa acggtaaaag
    15661 aaacccacat cgcatttgac gatgaagaaa tcgaacgtga tattttttgt ataggagcct
    15721 caatcgtgaa aaacgaacaa atcattggtg catttagtgt aagtatgccg aaatatcgtt
    15781 taacagatga gacaaaagaa caaataatca gtgccctcaa gcaaacaaaa agcgaaatcg
    15841 agcaaagaat tcaaaaataa tgaaaaaagc gtttgcaatt tttcttttct gccgtataat
    15901 atgggtatag atttccaagt tataaaataa aatttctaaa taggaaatag gtggagaaga
    15961 tgaaaaaaat tgatatttta tctcgattga aaaacgccgg agtgattgcg gtggtacgcg
    16021 gaaaaagcaa agaagaagct ttgaatgctt gccacgcaat catcaaaggt ggtctaactg
    16081 gtattgagtt gacttttaca gtccctcaag cagatcaagt aatcaaagaa cttttgtcat
    16141 tctacaaaga ccaaccagaa atagtgattg gcgcaggcac agtattagac gctgtcacag
    16201 ctcgcttggc tatcttagct ggcgcagagt atattgttag cccttccttt gatcaagaaa
    16261 cggcagagat gtgcaatctg tatcaaattc cttacttacc aggatgtatg actattaccg
    16321 agatcaaaac agcgctgaaa agcggggtag atatcgtcaa actatttcct ggaagcgctt
    16381 atggaccaag tatcatttct gccttcaagg cgccgatgcc tcaagtcaat atcatgccaa
    16441 ctggcggtgt gagtctggac aatatgaaag agtggttcga tgctggtgtc gtaacagtcg
    16501 gagtaggtgg gaacctttta gcacccgcag ctacaggaga ttttgacaaa gtaacggaag
    16561 tcgcgcaaca gtatgcagca aaaatgaaag aaatcaagag gtagctgctt atgggaaaag
    16621 tagtgacact cggagaaatc atgttgcgat tgtccacaga gcagggaacc cgtatcgatt
    16681 caagtgaacg attcgctgcc cattatggcg gaggggaagc aaacgtagca atttctttag
    16741 ctaattacgg acatgaagcg atttttgcta caaaagtacc tgataacgca ttgggaaaag
    16801 cagtcaaaaa gcacttgcaa tgttatggag tagatacgaa acaggtatta tctggtggat
    16861 caagactagg tacttattat atggaaactg gtagtggcga acgcgccgcc tcagtgatct
    16921 atgatcgtgc cggatcaagt tttgctgaaa tggatcattt ggaatggtcg ctagaggagt
    16981 tattccaagg aatagatata tttcatgttt caggaatcac cccagctttg tctaaaaaat
    17041 ggcaaaaaat gacgaaacag gtgatagaag ctgcaaaaaa agctggctgc ctgatcagct
    17101 tcgatatcaa ctatcgtgga aaattatgga cacagcaaga agccagcgaa gtaatccatc
    17161 agcttttacc tttagtagat atctgctcag ctggaaaaat ggatgctctt tatttgttag
    17221 gtatcgagaa agcgccagaa gcagaagaac aacctctaat ttattattat caaaaaatgc
    17281 aggaaaagtt cccgaatatt caggtgtttt attcgaccaa gcgtaccgtt cattcagcaa
    17341 gtgaaaatga attgaagggg acattgtgga tgaaccatga atatgtggaa tcccaagtcc
    17401 atagaatcac cccaatcgtg gatcgagtag gtggcggaga tgcttttgca ggtggcgtgc
    17461 tccatgggat tttatccaat atgtctccgc aagaaatcat tgattttgct acggcagctt
    17521 ctgccttgaa gcatacgatc catggggact gcaaccaatt tagtcaagaa gaagtcgcac
    17581 aatttttagc atgcggctct ggaaaaatca ttcgatagaa ggagtagaag aaaatgcaag
    17641 aaatggaaac acgttataca cacagtccag aagatatccg tcattattca acggaacaat
    17701 tgagaaaaga atttttagta gaaaaggtat ttgaaccagg gaaaatcagt ttgacttata
    17761 cgcacaacga tcgaatgatc tttggtggag tgacacctac tgaaaaagaa ttagaaatca
    17821 cattagacaa agaactaggt gttgattact tcttagagcg tcgtgaacta ggtgtgatca
    17881 atatcggtgg accaggattc atcgagatcg atgggcaaaa agaagaaatg aaaaaacaag
    17941 acggatacta tatcggaaaa gaaacacgac atgttgtatt ttcttctcaa gatgctgaaa
    18001 acccagctaa attctatatt agttctgttc cagctcatca taaatatcca aatgtaaaaa
    18061 tcagtatcga ccaagtaaaa ccaatggaga ctggtgaagg cctgacactg aatgaacgta
    18121 aaatctacca atacatccat ccaaatgtat gcgaaagctg tcaattacaa atgggttata
    18181 cgattttaga accaggaagc gcttggaata cgatgccttg tcatacacat gaacgtcgta
    18241 tggaagctta tgtctacttt gattatgcca atgaagatac acgtgtgttc cacatgatgg
    18301 gcaaaccaga tgaaacaaaa catttagttg tagacaatga acaagccatt atttcgccaa
    18361 gctggtcgat ccattcaggt gtaggtacaa gcaattactc attcatctgg gcaatgtgcg
    18421 gagaaaatat cacctataca gacatggata tggtgcaaat ggatcaatta aaataagcat
    18481 aagggagaga aaatgatgga atttagtatg gatatgtttc gtttagatgg aaaagtagca
    18541 ttagtcaccg gtgctgtcta tggtattgga tttgagattg cacgctcttt agctacagca
    18601 ggagcaaaaa tcgtatttaa taacttgaat caagaatcag ttgatgaagg tatagcgcat
    18661 tataaagaag caggcatcga tgcaaaaggt tatgtctgcg atgtgaccga tgaagaagcg
    18721 gttcaagcga tggttcaaca gatcaaagaa gatgtaggtt cagttgatat tctagtcaac
    18781 aatgcaggga tcatcaaacg gacaccgatg atcgaaatgg atgcggcaga cttccgccaa
    18841 gtaatcgacg tggatttaaa tgccccattc atcgtttcaa aagcagttat tccggatatg
    18901 atcgaaaaag gcggcggtaa aatcatcaat atctgctcca tgatgagtga attaggacgc
    18961 gaaacggtca gtgcttatgc tgcggcaaaa ggcggattaa aaatgctgac gaaaaacatc
    19021 gcttctgaat acggtcaata caatatccaa tgtaatggaa ttggaccagg ttatattgct
    19081 acaccgcaaa ctgctccatt acgcgaaaca caagaaaacg gtgaacgtca tccattcgat
    19141 caatttatcg ttggacgtac gccagcagca cgctggggcg agccaatcga tttggctgga
    19201 ccatctgtct tcttggcttc tagcgcatct gatttcgtta acggccatgt cttgtatgtg
    19261 gacggaggca tacttgccta tatcggaaaa caaccttaaa agagctttag tacactgttt
    19321 agtggagagg ttgtgaaatc agcggattga acgaataccg aaggacagct gattaaacat
    19381 catttattcg aaaaaagagg ctgggacaga agcatttaac tccaagaaat aagaaggaat
    19441 tcacgaaaat tgcttttcaa atttttgtga atttcagctt atttccgaag gagttgcttt
    19501 tacttccgcc gtttgtacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc
    19561 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
    19621 tccttcactg gtccggaaaa gatcagagat tgttggtgta gggccgcggt attcgttgcg
    19681 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
    19741 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cacggcattt
    19801 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
    19861 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
    19921 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
    19981 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
    20041 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
    20101 gtttgagttt cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
    20161 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
    20221 gcttctttgg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
    20281 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggacttt caaggcagcg
    20341 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
    20401 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
    20461 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
    20521 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
    20581 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
    20641 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaagaatgc
    20701 cagatttctg gtcaacaatt acgattccgc aatcgccccc acctgcacca gaagattttg
    20761 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
    20821 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
    20881 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
    20941 tattttttaa gaaaagctga taagccacca ttttatcttc tctcgaacgg tgaacttgat
    21001 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
    21061 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
    21121 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
    21181 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
    21241 tggctagtgc tgcaatcttg aaaatctcca actgagataa attcaacgcg taaaaaacat
    21301 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
    21361 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
    21421 tttcttgtgc atacttttca gtcaagcgaa tagcagcaag gatataatga aaaggatttt
    21481 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
    21541 attgtgcaga ttggatactt ccgacttttc gtgcagattc tacagtgact gtcacgaatt
    21601 gatcgactgc agcgataact gctggatggc ctgtttcaac aactgcatat tctccggcaa
    21661 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
    21721 caattcctgg accagcatgt gcagtaatta cttgttcttt gctaaataat ttggtaaatg
    21781 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
    21841 ccatcgtaaa gtagcatgga atgccttgct ttctcaattg tcgcaccgca tccatcgctt
    21901 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
    21961 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
    22021 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
    22081 atgtttcgac tgtccgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
    22141 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
    22201 aagaacttaa gtcgtcatgg cctttttccc attcgacgaa accaccgaaa atacttcggc
    22261 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcatct aatccaagct
    22321 taagagcagt attgcaagcg cctgctaaag cagctagccc gctggcagag gaggctaatc
    22381 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
    22441 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgcaagg
    22501 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
    22561 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
    22621 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
    22681 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
    22741 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
    22801 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
    22861 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgattgtga ttcatccatt
    22921 gtctgtgcca acgtttctgg tgaattttca atgattgctt gttttgcttg cttcgtcaag
    22981 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
    23041 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
    23101 gcatcgatgt tcaaagaaaa gtagtcgaaa ggatgccctt ttgtaaaata aatcggctgc
    23161 aagctgctag tagcggctgc atcgattcca ctaggattac catgggcgat tttttccgaa
    23221 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
    23281 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
    23341 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
    23401 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
    23461 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
    23521 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
    23581 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
    23641 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
    23701 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
    23761 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
    23821 actttttttc ttctttgact agaaaaaaat acaattgaca ctactagata ttgggggtat
    23881 atttaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
    23941 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
    24001 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
    24061 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
    24121 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
    24181 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
    24241 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
    24301 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttggagcgta
    24361 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga tccaagtcta tacgattatt
    24421 acaaagaatt gaacagacgt tacgatcaat ggtatgaaga atacaaagaa agtccaaaaa
    24481 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
    24541 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtagcc atttgattta
    24601 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
    24661 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
    24721 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
    24781 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
    24841 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
    24901 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
    24961 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cgaatgccag
    25021 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
    25081 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
    25141 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
    25201 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcattggct atttatcgca
    25261 agttgtcaga aaaggaaaaa gaagagttgg acgatgcgct gatcgaagca cgacaagcag
    25321 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
    25381 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
    25441 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
    25501 ccgttttcgg atggggaatc gccttgatca tcagtttggt cttatctatt tggatgctta
    25561 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
    25621 gatcacaagt gcttgctctt ggttgacgat tagtcccgcc aaagaatctt gagataaagc
    25681 gattgctgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
    25741 cgaaccggtc aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
    25801 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
    25861 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
    25921 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
    25981 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaaac
    26041 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
    26101 gagatagctt tggtcacccc cgttgatcag aagaccagag aagatctcct catcttctaa
    26161 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
    26221 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg ctagaaagtc
    26281 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
    26341 attagttttt gctatactat gatggttgag aaataaaagc agaagcaccc gcttctcgcc
    26401 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
    26461 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
    26521 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
    26581 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
    26641 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
    26701 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
    26761 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
    26821 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
    26881 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
    26941 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
    27001 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
    27061 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
    27121 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
    27181 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
    27241 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
    27301 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
    27361 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
    27421 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
    27481 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
    27541 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
    27601 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
    27661 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
    27721 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
    27781 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
    27841 ataacgtata agtctaaaat agcataatta aggtttttta attgaaaact gaacatttat
    27901 ggtcaccctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
    27961 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
    28021 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
    28081 atggtcggct ttgccatcat ggctactgta tggatgggat tagcgctagt cggaaacacg
    28141 ttttcaccaa acccggcgat ggaagggcaa ggagtaattg atgtgttgac gcatatctat
    28201 acgacggggc cagctgagta cgccaaatcg atcttggtaa atgtcttctt tggtgccttt
    28261 ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc tgattcgaaa ggtagttgaa
    28321 cttggagggg acaagccaag aatcacgatg agcttactat gtattgtaac cgctgttata
    28381 ttcatgtcga tgacaggaat cggaccagtg atttctattg cagttattgt gttgccaatc
    28441 ttgatgtcat taggtatttc agtaccggtt gcactgttct catttatggg ctcgattatg
    28501 gctggaatct ttgctaatat cgtcaatttc aaacaatatc aaacgattta tgctggtttc
    28561 aatccagcgg ctgaaagcta tacttataat gactatttcc aaattggtat gattgggatg
    28621 attgtaagct tagttgtggt actaacggtc gctaatatat caatgaacaa aaagaaacgc
    28681 tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg caccaatgat ctcatggtta
    28741 gccgtattat taccagtttt aggggtcgtt cttttagatc ttccaatcat cttaggtttt
    28801 attctagcag gtatctgggc attgctgttt actggaaaat tgcgcggagg ttataaagaa
    28861 atctgtcgtc agtttgcgaa attatttaca gatggcgcag tagatgttgc gccaatggtt
    28921 ggtttcttga tgaccttagc gatgttcaat aattcagctg cgtatgcttc tccttatttt
    28981 tcagctattt ttggagattg gataccgcaa tcaccattag tcttggcaat cgtatttgct
    29041 atattgacac cactaggatt tttccgcggt ccaatgaatc tagtcggttc aggctcagct
    29101 attttagctg tcgtattagc tgttaatcca acgatgtcgc cagccttttt gttcccgtta
    29161 tttgcaatca caacgattgc tcctcagcat ttagatatta cacaatcttg ggttgcttgg
    29221 ggattaggtt atacaaaagt aacttctcga gaatatatga agaaatccat cccaactggt
    29281 tggatcatcg gagcgatctt atgcctgatc acgttcttat tgtacgggaa cgcctagtaa
    29341 acaaaacgtc gataaaaacg attagatgaa tggaggaaaa gaaaatgaaa cgttttgtgc
    29401 gaatgggaat tgatgttggc ggtacacata cgaaagcagt cgcaatcgat aatgcgacgc
    29461 acgaaatcat cggaaaatct tcagtgaaaa caacccacga tgatgtccgt ggagtagccg
    29521 caggtgtcgt tcagtctttt caaaattgtt tgctggaaaa taatatcagt ccagaagatg
    29581 ttgtgtttgt tgcacattct accacacaag cgactaatgc actgattgaa ggagacgtag
    29641 caaaagtcgg cgtaatcggg atggcaaaag gtggattaga aggtttttta gcaaaacgac
    29701 aaactagatt gaacgatatc gatttaggga ataagaaaaa aatagagatt gtcaatgcat
    29761 tcttacctgt caaacactta aatgtagacc gtgtttcaga aacgatttct tctttagaaa
    29821 gagaaagagc ggaggtcttg gtttcttcta tggcatttgg agtagataat ggggaaccag
    29881 aacgagttgt ttatgaagca gcatcggtaa aatcgatccc tacaacgatg gcttctgaca
    29941 tcacaaaatt atatggattg acaagaagaa ctcggacagc agcaatcaat gcatctattt
    30001 taccaaaaat gctagatacg gcaacctcta cagaagattc cgtacgagaa gcaggggtca
    30061 atgtttcttt gatgattatg cgcggagacg gcggagtaat ggaaatcaat gaaatgaaaa
    30121 aacgacctgt tttaaccatg ctttccggtc cagcagcatc agtcatgggc tctttgatgt
    30181 acttgagagc gtctaatggt gtttattttg aagtaggcgg aacaacaacc aatatcgggg
    30241 tcattaaaaa tggacgtcca gcaatcgatt attcaattgt aggtggccac ccaacatata
    30301 tctcttcatt agatgttcgg gttctaggtg tggcaggcgg ttcgatggta cgggctaatc
    30361 agtcaggaat catcgatgtc ggtccacgtt ctgcacatat tgccggtcta gattatgcag
    30421 tatttacgga gacagaaaaa atcaaaggtc ctaaggttga atttttctct cctaaagaag
    30481 gagaccctgc tgattacgtc aaggtagtga tggaagacgg agaagaagtg actattacaa
    30541 atacatgtgc agccaatgtc cttggattag tgcaagaaga acatttttct tatggaaatg
    30601 tcccatctgc acgaaaagcc atccaagcat tagctgatta ttgtcatacg acggttgaag
    30661 acatcgcaga acagatcatg gaaaaatcct atgctaagat tgaaccggtg attttagaat
    30721 tggcagacaa gtatcacttg gagaaagatc agatttctct tgtaggtgta gggggcggcg
    30781 ctgcctcatt gattacttac tttagtaata aaatgggtgt gaaatattcg attcctgaaa
    30841 acgccgaagt gatttcttct attggtgtag cacttgcaat ggtccgagat gttgtagaac
    30901 gtatcatccc ttcgccaagt aaagaagata tacgatcgtt gaaaaatgaa gcgatgaata
    30961 aagcgattga atcaggcgct acaccagaat cgatcgaagt ccacgtagaa atcgatccac
    31021 agacttcaaa agtaacggct atcgctactg gttctacgga agtgaaagca accgatctaa
    31081 ccaaggagat cacaacagaa gaagcactag agcttgctgc agaggatatg cgattaaata
    31141 aaaatgaagt ttgcttattg gaaaatacac ctttctttta tgtatgcggc gaacagaatc
    31201 gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa aggatttatc aaggtccaac
    31261 gtggacatgc ttcatgtatg aaaacaactg cagccaatta tatgacagct gtcgaacaac
    31321 tttgggaaga tatggcagtt tatcaaactg aattgattgc ccgaccagaa ttttatctct
    31381 gcttaggagc aagagtctct gattttacag cgacggatct ggaacaacta cagcttttga
    31441 tggatctgga agtatctact atggaaccgg aggaagaagt gatcgtcgtt gccggaaata
    31501 tcaaacaaac ataagttaga agagttacta aacgaattac aacagatccc cgaagaaatt
    31561 tggggattct accagtttcg gcgagattta ttttggaaaa agattccttt gtccaagcag
    31621 aaaattctta tacaacaatc aatcgattgt ggtatcgaaa cggcatgtag catcaaaaaa
    31681 aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa tggctgttcc aatcgtttcc
    31741 tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa cttatgcaga agatgaaggg
    31801 attcgattga tgacggagcc gttagaaaaa ttaaaatgtt caggactagc ttcgatctct
    31861 aaagaaacag cgcaagcttt gattattgga catgaactat ttcaccacat cgaagcttct
    31921 gttaagggaa tttatacgca aagcgaaaaa attgtgctgt ggcggttgcc tttttataca
    31981 catcaatcaa acattcgtgc attgagcgag atagcagcga tgagtttttc aaaagagatg
    32041 aatcaaagcg atttttcgcc ttatgtatta gaggcagtct tactttggcc atataatgaa
    32101 attcgtagcc aagggatctt agaagaaata aaaaaaatag aaaaaagatg tacggagtat
    32161 gatttagatt acaaataaag agactgtgac aaaagtcgtg tcacagtcct ttttttccga
    32221 ataaacgatg ttctcctgtc aactcctcgg tcacaagtca aaatattcaa caaacatgag
    32281 aagctgtttt ttgaaattgt tccttgtgac tcggagatga acggcaggct cacgacctct
    32341 tggaaaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag ttaaaaacat
    32401 aagaagctat ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
    32461 tctcaataaa cggtgtgcaa tcagctgacc ctcggtatta gctcaaaaaa aacggcaaaa
    32521 tatgaggaac tattttgccg tttctttctc taatactcag gtcaaaacgc tgattccaaa
    32581 acctcttatt tgatgggtct gtcctgaata tcttcaaaat aatcggggta attatgaatc
    32641 acgtattctt gctcctcgat aatcatatgt aaatgcatcg tgcaaagaaa tccgacttca
    32701 tcaaagttat gatcaatcat tgattgaagc attgtctgat gttcatctaa tactcgaccc
    32761 cagtctaatt ctgaaatcgt caaacgtaac catctgaaac gcgtactata actttctagc
    32821 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    32881 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttg ttctagtatt
    32941 ttttctagtt tagcttgatc ttgcttggtc attttcgcac tgcattcttg caggatcggt
    33001 ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc gatttttgaa
    33061 acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag ttggatcagt
    33121 gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt atcggagatt
    33181 ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc ataagcttgc
    33241 tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa tctattataa
    33301 cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa agccctgata
    33361 ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg tacctcttgg
    33421 atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt tttgtctttg
    33481 aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt atcgatgtaa
    33541 ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt cattatacga
    33601 actactcgac acaatacaag aaaaatgctt agacggtctt tattaagaat ttacatcatc
    33661 caaagaagcg tacgcttgga ataactgatc taactccgga ttgactttgc tcagcttcac
    33721 taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag tttttaattt
    33781 tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgtccccg tatacttttg
    33841 gatagttgga tggatgctca ctttttctcc gtaatggatc atttctttat agctgcaatt
    33901 tacttccaga acggggatga tgatcccaga ttcttcgatt tttttatagg gaaaccctag
    33961 atgtaccaat aaggctaccc gagcctcttc aaaccagcgg atataattgc tgtgatgaat
    34021 aatccccatt tgatctgttt cataataaaa aggttctcgc acataacctg taaatttctc
    34081 catgactcat gcctcgtttt ccattgattt tttacaaaaa taattcatcc atttcggaag
    34141 tgcttcgctg atttgtgtcg gcaaaacaat atattgttct tttgccagct ggtctccaat
    34201 gaaactatga agataaatag ctgctgcaat cgtttggtca ttttgaggaa actgggctaa
    34261 aaatcctgtg atcatcccag ctaaagtgtc acccatgccg cccgttgcca tagcagcaga
    34321 acctaaagga ttggtgtaga agtgctggtg accataaatt tttgtctcat gacttttcaa
    34381 aacgagcaca gcaccgattt cctgttgctt ctttttgttt tctttatccc gttgttcttg
    34441 tatcggtaga gaactcaacc gttgccactc catttgatga ggtgtaagta cagtttgttc
    34501 gggatagggt agcaaaagat gattagcagc aagtaaactt aaagcagagc catcaatcac
    34561 cagccattgt tcttttgatt ggttttctag tacttgtttg aaacgttcca aactcttaga
    34621 agaagtaccc aatccgggac caatcaagag tacatctgct tgctttatta cggaaagaaa
    34681 ctggtcagta tccgtccaaa ctacaatcat tgcttctggc agatgactgt gaagagcact
    34741 ccgatttttt tcatcagtta ctacagtcgt gagacccgct ccgctgtgta cggcagctaa
    34801 agcactcata atgattgctc cgccatattg ttcattgccg ccaatcaaaa cgatacgtcc
    34861 aaatgttcct ttatgtgaaa tttttggacg aggtgtgatg actcttttca ctagttgttc
    34921 gtttatcagc atctaaaaac ctccttttac tcattatagc gtatattttt tttagagaaa
    34981 aacagataag aaaaggaatt gtcagatttt ttgaactatt tttttctgta atttgtggta
    35041 tcatgaacac gaagacttaa ataagtggag gattaacttt atgacaattg attggcaaaa
    35101 agaagtagaa gcacgtaaag aagacttgct tgaagacttg aaaaatcttt tacgtgtcaa
    35161 cagcgagcgc gatgactcaa aagtaacacc agatgcacca tttggacctg gacctcgcga
    35221 cgcattgaaa catatgttag cttatggcga acgtgatgga tttaaagtaa aaaatgtcga
    35281 taactatgca gggcatattg atcttggcga aggagacgaa acgctaggga tatttggaca
    35341 catggatgtc gttcctgcag gagatggctg ggacacagat ccttatgaac cagtaatcaa
    35401 ggatggaaag atctttgcac gcggatcaag cgatgacaaa ggtccaagta tggcagctta
    35461 ctatgcgatg aagatcatta aagaacttga cttgaaacta tcaaaaaaag ttcgcttcgt
    35521 agtcggcagc gatgaagaaa gcggctgggg cgatatggct tattatttcg aacatgaaga
    35581 agaaccagat tttggtttct caccagatgc tgaattccca atcatcaatg gtgaaaaagg
    35641 aaatgtttct ttagctttac gcttcaaagg cgacaatgca ggagattatg tcttgaaatc
    35701 ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca gcaactgcag cacttcaagt
    35761 accaagtgca gatgcagcta tcgcgatgga agaagcattt taccaattca tcgaagcaaa
    35821 tccagtaagc ggaacgattg aagcggacaa tacgtatgta aaaatcgaat tagttggtaa
    35881 aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca ggttctttct tggcattgtt
    35941 cttagataac tatgaattcc taggatctgc taaacaattt atccatgtag cagctgctta
    36001 tgtccatgaa gatttttatg gagaaaaact aggagtcgct tatgaagatg aaaaaatggg
    36061 caaattgaca atgaatgctg gactatttgc ttttgaagaa aatggtacag aggaagcgaa
    36121 cttcatcaat atgaatttcc gttttccaaa aggtgtaaca gtagacggat tgcaaagtga
    36181 catcgaacaa acagtggggc aagaaggcgc aacagttact cgaggtgctc gcgtaatgga
    36241 accacattac gtaccaatgg atgatccatt ggtggctaca ttattgcaag tctatgaaga
    36301 ccacactgga gaaaaaggct acgaacaaat catcggtggc ggaacttacg gacgtttgtt
    36361 gaaacgtggc gttgcttatg gagcgatgtt cccaggatat actgatacaa tgcatcaagc
    36421 aaacgaattt atgagtcttg atgacttgtt cagagcaaca gcaatctatg cagatgccat
    36481 ctatcgtttg gcgaaataat taattaaaaa gaggatcaag aattgcaaat tatttttgca
    36541 gtcttgatcc tttttttact ttctactgtg gtattcatga taaacatgat atagattcaa
    36601 ttcatattct ttcttagcgt tcgtgacgac tgtatccaaa atcaaccctg ttactaatga
    36661 aagaagtgct aaagtcatca atccagtact taaaatcgca gaagggatgc gtgtaataaa
    36721 gcttgtcagc atatattcgc gaatcacagg aatccccgtg accaatccaa ataaaaagaa
    36781 gaagatggtc caaatcccaa agaaaagcaa aggcttataa tctttaaaca ttttaataat
    36841 catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc tgccttcagg
    36901 acgatcacga taatcgattg ggatttcaac gaacttgaat tttttatcta aagcatggat
    36961 cgtcaattcc gtttcgattt gaaaaccaga actcataata ggaaaacttt tgacaaagat
    37021 acgattgaat cctcgatagc cggtcatgac atcaagaatt tttgttttat acattctagt
    37081 aattgtattt ctaacaaggt tatttccaaa atcgtggaaa ggtctcttat tctcatcaaa
    37141 gtaggttcca tttgataggc gatcaccaat gaccatatct gcttcaccag aacgtaattt
    37201 ctcgagcaaa ccatgtacag cttctgcagg ataagtatca tcgccatcta ccattaaata
    37261 atagtcggca tcgatatcaa agaacatttg gcggatcaca tttccttttc cttgacgagg
    37321 ttcttttttt acgattgctc caccttgaac ggccaattcg tatgttcgat cggtcgaatt
    37381 attatcatag acataaatat ctgcttctgg taattctcgt ttaaagtctg caatgactgt
    37441 tgaaattgtt gcttcctcat tgtaacaagg aatcagaaca gcaatttttt cttgttgttc
    37501 catttttcac tctccttaac tatagaaaac aaaagctaaa ttaaaaatac ctcataatca
    37561 aaggaaatgc tagtttttcc cgctaaatat catttattta aaaagaaaat actggaatat
    37621 atgtttggaa agctctatct aaaaaaccac ccactacaaa agaaggacat gatacctctc
    37681 ttttgaatag tgggtgcgta ttcgttctct cacttttttg acgtattaat ctgttgcacc
    37741 tttttttctt gaaatttcta atacggttcc tgtatgagca tctgctaaaa attcataata
    37801 aacagtttcg ccatcttcta ttcgagaaag acctccccga taagcactcg accgaacggc
    37861 gaatttccta ataggttcct tttcaaaaga gatccaagaa ccttcgattg caccttcttt
    37921 tagaaaagat gacttgatat tttcaagtac ttggtctgct gacaatactt ttttgcgatg
    37981 ataccaaatc gttgaagcta ccccgctcaa taatcctaga ccgattccaa ctgctaaccc
    38041 gcctttaaaa tagcttagtt cgctttcttc atacatgtgg acatacctcc ttcttctcac
    38101 ttgttactcc ttgtgcttat tttaccacac tctcatattt tctttgaaat aaataaccgc
    38161 taccagaaaa aataagctat aataaatacg atcggtctgc agaaaaaatc atattttatc
    38221 cgcagatcaa tataagtctg agaggagttt agtctgaaat ttagacgaat agcatatgga
    38281 agaaaaaaca tttcaacgaa tcaaagagtt aacagaatta caaggaacaa gcggttttga
    38341 acaagatgtt cgcgcttata tggaaagtca tatggaaccg ctggtagatg agttacagtt
    38401 agatggtttg ggcggcattt ttggtctgcg ccatcatgaa gaagcggatg caccccgtgt
    38461 aatggtcgca gcacatatgg atgaagtcgg attcatgctg acacaaatcc aagaaaacgg
    38521 gctttttaaa gtcgtacctc taggcggctg gaatccttac gttgtttctg ctcagcgttt
    38581 tacacttaaa acctctactg gtaagaatta tccatgtatt tcttcttccg ttcccccaca
    38641 tttattgcga ggaacaagcg gacaaaaatc agtagaagta accgatattt tgttcgatgc
    38701 tgggtttgaa tcaagagaag aggcaatgag ttacggtgtc cttccaggag acacaatcgt
    38761 tccttacgct gaaacgatca aaacagccaa cggaaagaat attatcagta aatcatggga
    38821 caaccgctat ggctgtacaa tggtattaga agctttagaa gcgttgcaaa atgaaacatt
    38881 aggtcataca ttgattgcag gcgcgaatgt ccaggaagaa gtaggcttgc gtggttctaa
    38941 agcttctgtc aataaattca agccagatct gttctttgca gtagactgct ctgcagcaga
    39001 tgatacagtg acgaaaaatg gcacattcgg acatttagga gaaggtacat tgatgcgtat
    39061 ccaagatcca ggtttgatca tgcttcctcg tttgcgcgag tacttgctgg atatcgctga
    39121 aacaaacaat atcccttatc aatactttgt ttcaaaaggc ggtacagatg caggtgctgc
    39181 tcatacacaa aacgaaggaa tcccaagtac agtgattggc gtagtgggac gttatatcca
    39241 tacgcatcaa acgatgttca gtattcgcga ttttgaagct gcacgagaaa tgttgattca
    39301 aacactgaaa ggtttggata aatcaacagt aaatacgatc gtatacggaa aataatgcga
    39361 tagtgaaact ataaaatctg gcaaaagatt gctttttgcc agattttttt tataaaaaga
    39421 ggatcaatgt ttgttagttc gaaagaaagg tgtataattt aattgaaaaa tacataaaca
    39481 gaaagtagag gacaaagaat gattatccca aatagtttag aagaattagc aggctacgta
    39541 gaaactggaa agagtgtatt tttctttact gccggctggt gcggagactg ccgttttatc
    39601 aaaccgcaaa tgcctgaaat tgaaaatgac ttttctgatt ggcaatttat tgaagtagac
    39661 cgtgacaagt acatagatgt agccgctgaa tggaacattt ttggtatccc aagttttgtc
    39721 gtgatccaag acggaaaaga attaggtcgt ctagtgaata aagatcgtaa aacaaaacag
    39781 gaaatcgaat cgttcctaga aagtgtaaca aaaggctgac atctgttagg aggggtcaag
    39841 atgaatcaag aatataagaa tattttagta ggtatcgacg gtagtgaaca agcacttgct
    39901 gctttcaaaa aagcggtaga agtggctcgc cgtaataatg ggaaagtata tgtagcaaac
    39961 gtaatcgatc agcaatttta taactttatg ggctatgcac ctattgatca aaatctagtc
    40021 gatcagcaaa ctgaaacagc caaggaaatg atcgaagaat gtaaagaata tgggaaatct
    40081 gttgattaca caaatataga aggaattatt gcatatggtt ctgttcgtga atccatggca
    40141 gtcaaattgc caaaaaaata tgagatcgat ctaatcatgg ttgggcagtc aggtctaaat
    40201 gcagtcgaac gctttatgac aggaagtgta gccagctacg tgatacgtca ttcaccttgt
    40261 gatgtcttga tcgtttctga cgaagaagaa cagccaagta attaagcaag tggaacttgt
    40321 acgacctttt taatcaatta actaaaacag taaagggact gtgacaaaag tcttgtcaca
    40381 gtcccttgtt acgaataaat ggtgttccag aagaggcttc ttcggaaata aagcatgaaa
    40441 aaaatttcat gcagtgaatg caattttttg ttaagataaa tctcagcaaa ggataatgga
    40501 ggaaagaaca tgatttttgc ttataacaaa gcacacgtcg gagacacctt gatggttatt
    40561 atagccgacg ataaaggaac agaaaacact gttgaacgca aatggaatgt tgctcggatc
    40621 aaagatgaac aaggtcaagt tgttgcttgg aactttttcc atatctccga tcatctaaca
    40681 atcgaaggga acggccaagt aagcttaaca gatgaggaat tagcgaaatt gaatcgattg
    40741 atcaaagaag caggattcga agaacagctt gaagctgatc atgaaccgaa gatcgttgtc
    40801 gggtacgtaa aaacatgcgt cccacatcct gattctgacc atttatcgat tactgagaca
    40861 gaagtagaca atggtacagt attgcaaatc gtttgcggtg cacccaatat cgaagctgga
    40921 caaaaagttg tcgttgcaaa accaggagct atgatgccag atggaatgat gatctggcca
    40981 ggaactttgc gaggggtaga tagctatgga atgatctgtt ccgctcgtga attgcatttg
    41041 ccaaatgctc cagcaaaaaa aggaatctta gtattgcctg aggatgccgt agtcggagaa
    41101 gcatttccaa aataaagagg ttgtgacaag tctttgtcac aacctctttt ttattaattt
    41161 tcactatttt gattttgttt taatgccgat tgatcgatcg tcaattcaac agtagttgtt
    41221 ttttctttcg agccttcata gtaagtgatt ttgacagaat ctcccacttt tttcttatac
    41281 aaagcagatt gaagttctac accagaagaa acttctttgt catcgatctt agtaatgacg
    41341 tcgtattgtt tcaatccagc tttttcagcc ggtgtagctg tttggacatt tgtcacgatg
    41401 acaccgttag ttacagatga aggaatcttc aagatttgtt cttgttgctg agtagaaacg
    41461 gcagataagt cgaccattgt gatacctaga gctggacgtg tcacttttcc gtctttttcc
    41521 agttgattga tgatattcac tacatcgtta cttggaatag caaatcccat tccttctacg
    41581 cttacattag aagtagattc agaagtactt gcgatcttac ttgagttgat accgataact
    41641 tgtccttcga tattaactaa aggaccacca gaatttccag ggttgattgc tgcatctgtt
    41701 tggatcgcat tgatattgac tgtttcgttc gattcattcg tacttgtcac ttgtcggttc
    41761 aaggaagaaa tgattcctga agtgacagaa tttgcatatt cagatcctag tggggaacca
    41821 atcgcaatcg caggctcacc aaccttcaga gcacttgagt caccaaaaga ggcaactgtt
    41881 tcgactttgt ctgaattgat cttcaacacc gctaaatctg aataagcatc tgttccaact
    41941 aattcagctt ttaccttcgt accgtctttc atcaagactt caagaccttg ctggccatct
    42001 actacgtgat tattcgtgac tacataagca gtattcccgt cttttttgta gatcacgccg
    42061 cttccttcac tataagcttc caaattacta tcatctgagc tgctactttc ttcttgctgc
    42121 ccgaatagtc cgccaaaacc gcttgattgg ttttgacttt gtaagttgat aacagaaacg
    42181 acagcgtctt gaactttatc taccgctttg gtgatatcag aatctacatt gactttgaca
    42241 ttttccacaa ctgtttcacc ggcagaattc tgattgccgc ttgtagcagt agaactattg
    42301 ttgccagtgc ccatcgctgc ataaaaaatg ccagctgtca gtagtcctcc aacgatccct
    42361 ccaacaagcc ccagacctaa cttacgccag aggctattat ttttactttt tttcatcttt
    42421 ggtgtcacat tttttctatc catgtgttcc tcctccaatt acattcattt atctactgtt
    42481 agtatagtct tatttgttga ctttagtcta ctttgagact gtgaactttt tatgaaaaac
    42541 tttaggaaaa atcattgttt tcagatgaaa atatttgatt ttctttttcc acagatactg
    42601 tgtaaaacta cgcggatagg attggaaaaa taagggataa aacatatcca cagcctgtgt
    42661 ataaagtgga taaattctta taaaacgtat gtttgcctaa tgaatgtgat gaaataaggt
    42721 aaaataacac tgttttctgt ggataaacct gtggatgttg tgcataactg ttggggaaat
    42781 ctttttaaca aaaatagcca aaataaacat atagacgaaa aaattgatta taataaacaa
    42841 agtgagcatg aagaccaaag gagagaaaac atgaatatca aaattatctc agtaggaaaa
    42901 ttaaaagaaa aatacctgat ccaaggtatc aatgagtatg tcaaacggct gaatgcctat
    42961 gcaaaaatcg aattgatcga agttcctgac gaaaaagccc cggagaattt aagcgaagcg
    43021 caaatgagac aagtaaaaga aaaagaaggg gaacggatct tagcgaaaat caaagaacaa
    43081 gaatacgtct atgccttagc aattgaaggg aaaaatccta caagtgaatc ttttgctaaa
    43141 caaatcgacc aattaggtat ccaaggaaaa agccaccttg tttttgttat cggcggctct
    43201 ttaggactca gcgaagccgt catgaaaaga agcaatgctc agatctcttt tggaaaaatg
    43261 acgtatccac atcaattgat gcgtctgatc cttgtcgagc aaatctaccg tgccttccgg
    43321 atcaatgcgg gggctccata ccacaaataa tgatattttt taagagaaaa aattatcc
//
LOCUS       DACCXW010000009        37519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCXW010000009 DACCXW010000000
VERSION     DACCXW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.009"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            7..684
                     /locus_tag="IVY71_000398"
     CDS             7..684
                     /locus_tag="IVY71_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984473.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            687..1814
                     /locus_tag="IVY71_000399"
     CDS             687..1814
                     /locus_tag="IVY71_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984474.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2014..2454)
                     /locus_tag="IVY71_000400"
     CDS             complement(2014..2454)
                     /locus_tag="IVY71_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7984475.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(2465..4498)
                     /locus_tag="IVY71_000401"
     CDS             complement(2465..4498)
                     /locus_tag="IVY71_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7984476.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4500..6203)
                     /locus_tag="IVY71_000402"
     CDS             complement(4500..6203)
                     /locus_tag="IVY71_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7984477.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6297..7247)
                     /gene="manA"
                     /locus_tag="IVY71_000403"
     CDS             complement(6297..7247)
                     /gene="manA"
                     /locus_tag="IVY71_000403"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7984478.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(7286..8311)
                     /locus_tag="IVY71_000404"
     CDS             complement(7286..8311)
                     /locus_tag="IVY71_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7984479.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(8520..9389)
                     /locus_tag="IVY71_000405"
     CDS             complement(8520..9389)
                     /locus_tag="IVY71_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7984480.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(10093..11985)
                     /locus_tag="IVY71_000406"
     CDS             complement(10093..11985)
                     /locus_tag="IVY71_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984481.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(11982..13709)
                     /locus_tag="IVY71_000407"
     CDS             complement(11982..13709)
                     /locus_tag="IVY71_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984482.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13725..14375)
                     /locus_tag="IVY71_000408"
     CDS             complement(13725..14375)
                     /locus_tag="IVY71_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7984483.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14579..14875
                     /locus_tag="IVY71_000409"
     CDS             14579..14875
                     /locus_tag="IVY71_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7984484.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            15067..15687
                     /locus_tag="IVY71_000410"
     CDS             15067..15687
                     /locus_tag="IVY71_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7984485.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15779..16945)
                     /locus_tag="IVY71_000411"
     CDS             complement(15779..16945)
                     /locus_tag="IVY71_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7984486.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16957..17310)
                     /locus_tag="IVY71_000412"
     CDS             complement(16957..17310)
                     /locus_tag="IVY71_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7984487.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17438..18460
                     /locus_tag="IVY71_000413"
     CDS             17438..18460
                     /locus_tag="IVY71_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7984488.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(18899..19333)
                     /locus_tag="IVY71_000414"
     CDS             complement(18899..19333)
                     /locus_tag="IVY71_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984489.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19443..21302)
                     /locus_tag="IVY71_000415"
     CDS             complement(19443..21302)
                     /locus_tag="IVY71_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7984490.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(21420..22580)
                     /locus_tag="IVY71_000416"
     CDS             complement(21420..22580)
                     /locus_tag="IVY71_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP7984491.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(22887..24311)
                     /gene="araA"
                     /locus_tag="IVY71_000417"
     CDS             complement(22887..24311)
                     /gene="araA"
                     /locus_tag="IVY71_000417"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP7984492.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24327..25025)
                     /locus_tag="IVY71_000418"
     CDS             complement(24327..25025)
                     /locus_tag="IVY71_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7984493.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(24982..26643)
                     /locus_tag="IVY71_000419"
     CDS             complement(24982..26643)
                     /locus_tag="IVY71_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP7984494.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26861..28261
                     /locus_tag="IVY71_000420"
     CDS             26861..28261
                     /locus_tag="IVY71_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP7984495.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28513..29592)
                     /locus_tag="IVY71_000421"
     CDS             complement(28513..29592)
                     /locus_tag="IVY71_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7984496.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29678..30637)
                     /locus_tag="IVY71_000422"
     CDS             complement(29678..30637)
                     /locus_tag="IVY71_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7984497.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30649..32172)
                     /locus_tag="IVY71_000423"
     CDS             complement(30649..32172)
                     /locus_tag="IVY71_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7984498.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(32182..32745)
                     /locus_tag="IVY71_000424"
     CDS             complement(32182..32745)
                     /locus_tag="IVY71_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7984499.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(32751..33614)
                     /locus_tag="IVY71_000425"
     CDS             complement(32751..33614)
                     /locus_tag="IVY71_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7984500.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(33628..34524)
                     /locus_tag="IVY71_000426"
     CDS             complement(33628..34524)
                     /locus_tag="IVY71_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7984501.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(34699..35970)
                     /locus_tag="IVY71_000427"
     CDS             complement(34699..35970)
                     /locus_tag="IVY71_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7984502.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(35960..37477)
                     /locus_tag="IVY71_000428"
     CDS             complement(35960..37477)
                     /locus_tag="IVY71_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7984503.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        10871 a         7840 c         6510 g        12298 t
ORIGIN      
        1 ctatctatga cattactgat caaaaacatg aacatgaaac gtggtaaaaa acgtgtgatt
       61 cgacaattga atcttttagt agaatcagga gaagtagctg ctttgattgc cccgaacgga
      121 tttggcaaaa caactttgct gaatggcatt gcccaactgc tgccaacaac ttatgatcaa
      181 ttgtcccttc acaacatctc tcctactgct tatacagatt atcgccggtc tttctttttt
      241 ctagaatcaa cgaatcagtt atccgactat ctatcggctg ttgattattt gactttgatc
      301 aaatactatt ggcagagcaa agtagaatgc caaactgttt tccaacgatt gaagatgact
      361 gagtacctta ctttgccgat tggaaaaatg tctctaggaa tgaaacaaca tgtcttgctt
      421 gctgcttact tgatcagtga ttcatctgtc atgttattcg atgaaccgtt gaacggatta
      481 gacccaggaa gtattgatat cttgaatagc ctcatcagac aatggcagaa agaggggaag
      541 acgattcttc tttcttccca cgctatcgcg aatatccaat cgatttgttc taaagcatgt
      601 tttctaaaaa atggagaaat cgtcacagaa tcctcaaata tggaagagat tttagctatt
      661 tatcgtaaat tatatgaggt gtaactatgt ctttacttac atttgaacgc aatctcttac
      721 taaaaaacaa agtaaacttt ttatttcctc tattgttggt cgtgctcttt gcattcccac
      781 ttttttttga tcataaactt gcttatacag aattcgatga actgaatcat aactatgaag
      841 agatgcaacg tttgattgaa acgttgaaag aagatgagaa tgagaaagaa tttgtggaaa
      901 gtttagagaa aagcaacaaa ctgatcgaag caatcttaca tgcaaaaaac acagggaatg
      961 tccagcaaat ggtcgaagca acttatcatt atgaaaaaga tatactggat cgactgattt
     1021 ctggtcaaag acaaggaatt ccaatcattg aacaacaaaa acgtgtcgag ttgctccgtt
     1081 atatgaaaga acatcagatc caaagatatt ctatttttga cttgccagcg catctctctt
     1141 tagcaaatta ctacgaaaat attttttcag gaatgatttc tagttttttg attttatgta
     1201 taaccgcctt gtttctttct tccatcatct cttatgaaaa aagaaagcag gtaatctctc
     1261 tagtcaatct attactggat agtatggtaa agaaacatag catccgtttt accatttatt
     1321 atggtgcagc tatgctaagt ctcgtgatgc catttttgat cgtctctata ctagtaataa
     1381 tcaaaaatgg tttgggagac ttccgttatc ctgtaggaac gatcattggt caggagatac
     1441 gaatcctgcc gatgtatgag tatcttttcc agagctttct gttcctgttg ttgtgggtgc
     1501 tctttttaag tacaatcagt tttcttctat ctgctttatt tgaacattct ctagtcaatc
     1561 ttttaggtac acttctttgt cttttcttag cagagtatcg cttgttttct tcgattggat
     1621 ggatagaatc aatcagtcat tacttgccaa cttcttatgt tgattttcaa aatgtgatca
     1681 taggaggaga tattttctcg ccactagctt cagaacaggt tactttcatg aatggtatct
     1741 tgacacttgg tatatggagt atcgttttat tattcatagg tatgggcact atctatataa
     1801 aaaagagcta ttaaaacaca aaaataatag gggcggtgac aaaaaccttg tcaccgtccc
     1861 ctatttcaaa taaacggttt tctagaataa atagttttca aaagcttgca tccgcagtaa
     1921 taagaacctc ccaacaaaaa tagctcctct tatttttatc ttggttgaag ttattacttg
     1981 atgcaaggca gcttttttta ctgtgaacat tggttatata tgtccatgtt caaaaatcat
     2041 ttggacttct tcaatggttt ccgcatcact gagcgccatt acttggtcga tgtctgaaca
     2101 gaaaaaggct aaatcttgga gcaccgctaa ctgatgttct ttttttaaag ccactacaaa
     2161 taagagtgtc gcgatttttg gttcctcttt tgttccaaag tccacaccat caggcacttg
     2221 gactaaatag attccttctt ctactacttc ttcatctttg tcagtcccat gtggcaaggc
     2281 tacgaaattc ccgatataga cactcgcttt ttcttgtctt tcgatcattg attggatata
     2341 tgccggtttg attccccctt gttgggtcat ccactggccg atctcggtca acgcttcttt
     2401 ccaattttga taggattgat cgagcaatat tttttctgct ggtagctgta tcatcttttc
     2461 accttcattt ctttatttct tcaatcagga ttttcgctaa caactgataa acgatttctt
     2521 tatttcctga gcggaagatc tcggtgttca agtcattcat gatgattgct ccactgatct
     2581 ttcctaaaag aatcccgtct ttttcttgga ttggcgaagg cgccagcata actaacatac
     2641 gggacaaatc gactggctgt ctatccatcc cttcaatggt caacggatgt ttcaaattga
     2701 aaatacaaaa gatcggttta gaaatcaatg agctggatgt atggaataac cccatatttg
     2761 tatgaggaat accgataggt gcttgttcat atcgcttaag cagccggcga tgaaccacct
     2821 cttcatccgt tactagtcct tcaggaaaat aaggaaataa gctgcgaatc gtttcggaaa
     2881 tcgtctcagg attatccagt tcttggataa aaaaacgacc gagtaattga ttgatttgat
     2941 ccatgaaatg cattgtttcc tcataagtat cttctatcat ccactcgtta ttgctgttat
     3001 acgtaatttt ttgccttttt ttcttctcaa tatgcgaaaa agcttctttt agctgcttga
     3061 cttcatcttc tagcagtagc ggggagacta aaatatattt tccaccgtat cctggtaaaa
     3121 tagatgtaga aataacaaca tcatattctt cttgtaaatc gatttttttt aaatcagcaa
     3181 tccgatagaa atgaatttgt tggatgaagg gaaaatacct tttcaatcgt agttccaaca
     3241 tgctagtaga agctaatcca cttggcgaaa atcctgcaat actgatattg ctttctttcg
     3301 gacttcgttc caatgaattc gcaaaatgaa ggaccatata agcgatctct tcttcggata
     3361 attttttttc ttgaaaaatc cgctctactg ctttacggat tgcattcgca acttcctcgt
     3421 attgaaccat gattttttct aaaatcggat tggtcagtgt ctcttcttgt aaaacggctc
     3481 ttgaaaacac accagataaa tgagtcaaca gcattttgta caaagtttca tcttcataaa
     3541 aatggacatt cgtctcttca cttacttctt gaatcagttg ttttactaaa taagcaagct
     3601 gtgtatcgaa cgtctcttca aagaaattct gatcaaatga gttcgtgaag tcatttaata
     3661 atcctgcaaa aaagacgatc tcatttactg gataaagttg ttttgtctca gaagcgatcc
     3721 gtgaaaacaa tcgtttagct gtctgtagta attctttgtt tgccagacta ttatatgtct
     3781 catttgtcag taaatgattt ttcgtcacac gctccatcgt caatgtcagc atcaaaacca
     3841 aatgttcaag ctttctatca cttagattgg tctttttttg gatcaattct tccccgatta
     3901 ctttgcgtgc aagagataga tgttgcggat caacgaatcg aaaaaaataa ttcagtgatg
     3961 catcgaaaga aatactatga aaaagttcat attcattgat ttctaattct aatacattcg
     4021 ctgtcagaag ccggcggtat ttttccggtc cttcgatctc gtatccctgg ttccgaatga
     4081 tttccaaagg cagttggcga atactttctt ccaactgttt gatatctgca taaaaagtgg
     4141 tattactgat gcgatatgat tccatgaaat gagcaagact caacggctct gtcgtcagta
     4201 acaactcggc taaaatcgcg tgttgtcgtt tagcagtgga taagtcatta cttttcccat
     4261 ctgtattttc ttttaattgc tctaatactt cttctgatgc ctcaatgaca aatgagcctc
     4321 tagctgcttt tttcaaagaa gcatttaccg atttcaatga gacttcaaga ttatctaatt
     4381 cgcggtaaat agtccgttta ctgaccttca ataaattcat cattttttgg atagatacag
     4441 gtgacgggct actgagaaat tctgctaata attgtttttc tctgggcgat aaatacatct
     4501 tatatttctc ccttcacgag tttttcgtat tcattcgtag ctacccaatg ttcaactaga
     4561 actagccgag ggatcttttc ttctagctgt tcagttacag ctagttctct ttgtgcgata
     4621 taaatcgttt cttcttggat tttgattttt tctattgatt cttttgagat aacaaactgt
     4681 tttttcgctt tttttgctaa ttgacttaaa atcgtcatgc ccatagtttg tgagccgcgt
     4741 ctgttgtttt tatacaaaaa tacaatatta gcaggcgacg ttttttgttc tgtgatatct
     4801 gctatctctt ttttttcttc ccgctttgga aattggatcg cttcttttat tcgtgacagc
     4861 aagatatctg tttccaaaaa gtgatccagt acatagattt gactttgagg tactttttct
     4921 cttgcaagac ccgctagttc atgttgcacg ataaccagct gatttacttg gttattcaat
     4981 tcatagatag agctgtatcc gacagaaaga tcgatttttt ctgctgctaa tttctttttc
     5041 agcagggcgg ctcccatagc acttgatccc atacctgagt cgcaagcaaa aatgacggaa
     5101 tgaatgatag atggttccaa gatttctccc ctttctttga ccgttttgtt ctctttttct
     5161 ttatttggta atttcaatag tatcgctgca caaatgaagg tcacaaatgt actgattgct
     5221 atccctatac ttaccccagc tagttgtgga taaggcgcat tggcaaaaat cacgagtagt
     5281 gacccaggcg atacaggtgc agctaatccc gtatctaacc attgaaatat caacgtgccg
     5341 cttgccccac cagccattac agctaaaaat agatacggtc gcagcaagac aaatggaaaa
     5401 tagatttcgt gaatcccgcc aatcgcatgg atcatcaatg ctccactcgc atttactttt
     5461 tcctgttttt ctgcaaaaca aaggatagaa agtaagatcc ctaaaccagg tccaggattt
     5521 gcctccatca aaaataagga agagtacccg actgtttgtg agaattcgat tcctaaaggt
     5581 gtcaatacac catgattgat tgcattattg aaaaatagta cttttagagg ttctaagatc
     5641 agactgacaa gtggtaacag attcattgtg actagccaag acacaacgga ggcgagcaac
     5701 aagtgaacac tatttaaaac tggttcaata aaaacgattc caattataca aataacgcta
     5761 ccagtcagtc ctactagtag gttgctgaca agcatttcat atccagcctt gaatttcaca
     5821 tggaatctag aaacaaattg ttcatatacg atgccggcaa gcggcccgat aatcatcgct
     5881 cctacgattt gtggagcatt ggaacgagcg atcaacccta aagaagcaat tgcaccggcg
     5941 actgcccctt tttgtccccc taccgatttc ccgcctgaat aactgatcaa gatgggtaaa
     6001 agcagctgga tcatccaatg gcatacctct tctaatgtgg tgtgcaaatc gccagacaca
     6061 ccttttgttg ctaatgacaa gattccccaa gcgataaaca gacttagatt tggcatgatc
     6121 atttcattta agtgatgtcc agcttttctc agttgtttca tagacacgcg actgctgttg
     6181 tttgctttcc atttgtattc catctttttc accagacttc tatttattga tggacggatt
     6241 ttatgtagaa aaagcagcaa acgaaaaacc gtctgctgct ttcagctatc tgggaactat
     6301 ttgcctggtt cagacgcaat gattgctagt tcaccttgga ccgcccactc agaaacgcca
     6361 tttggaataa tacagcttgt tcctttttct aatggatatg tttttccgtc aacgacaagg
     6421 tctcctgctc cttcgattac cgtcatcaat gtataagggg cttgtttttt gaaagatgtg
     6481 attcctttaa tatcccactc atatacattg aaaaactcag tttctaaata agtgacgatc
     6541 gatgaattcc cttttcggac ttctttgatt tgtaattctg gtgttttcgc agggacagtt
     6601 gttacatcga tcgattgttg gatatgcagc tctcttgttt gtccttgcgc atcttttctg
     6661 tcgtagtcat acacgcggta agtcgtgtca gaactttgct gtgtttctaa aatcatgatt
     6721 cctttaccaa tagcatggat cgttccgctt ggtacataaa agaaatcacc ttttttaact
     6781 ggtactttct tcaaaaggtc atcccagcgc ccatctttga tcatctcagc tagttcttct
     6841 cttgtctttg catgatgacc atagatgatt tctgcaccag gttcagcatc gatgatatac
     6901 cagcattcag tttttcctaa ttcaccttca tgtttcatcc cataggcatc atctggatgt
     6961 acttgtacag atagatcatc ttctgcgtct aagatcttga tcaataatgg aaacactggt
     7021 tctgaaggat ggccaaacaa ttccttgtgt tctgaccata gttcatctaa tcttttccct
     7081 ttgaattcgc cattttctac tacactgaca ccatgaggat gcgcactgat cgcccaatct
     7141 tctccgattt tatcgttagg gatatcaaaa ccaaagactg aacgtaaacg acttcctccc
     7201 caaatttttt cttgaaaaac tggttttaaa aacataggtt cctgcataat tcaaactcct
     7261 ctcatttttc atagctatta tagcattatt tcggtaatac cgctttctct ttcaaaaagt
     7321 tttcaacaat ttcatctcga catatgagat actccgcacg atgatcgaca ggatgtacgc
     7381 gatttgacaa aaatatcaac gcttcttgtg tttttatatc aatcaagaaa aacgtacctg
     7441 tataaccagt atgaaaaaga atcgcatgac cgtcttctac atcctttttt aagtcccatc
     7501 ctaaagaacg atagccattt tttttcggtg tttgatcttg cagtaaagaa tcaatcgtct
     7561 cttcttctaa aaaacgacca ttacggattt ttcccttgtt caaatacatt tggacaaaag
     7621 taatgctgtc tttcaaatca ataaagagac ccgcgttacc tgcatgttca gccaaaactc
     7681 ttgctttcgg atcatgagtt tgtccacgca aaacctcacc cgaaggcagt tgttctgtgg
     7741 gaacgatttt tttggaagga aaaggaagaa ataagctgtt catcattcct aatggttcca
     7801 atacttgttc ttgaaagatt tccactgcag tttcttggta aatttcctct agcatcaacc
     7861 caagaagaat cgtaccggca tctgtatatt ttacttcttt tcctagttgc tcacctggtt
     7921 ctagcttcaa ataagcggcg atcaattctt cctgagatag cttatctctg tttggtatcc
     7981 atgttttgat atcagaagta tgtgtcagta aatgacgcaa agtgattgtc tgattttcaa
     8041 attcaggaag atatttatga agcggttgat cgagttctat ctttccatct tctaatagac
     8101 gtaaaactaa tgaagtggtt gcaacgactt tcgtcagtga ggctgcatca aacaacagat
     8161 cttctgtcat cagctgcttt tcaggaacga cttgggcaaa gccggctgta aatgtttcta
     8221 cttgattttc ttttatgaag gcgactgcta cacctggata aaatccttct tccatctttt
     8281 tcaaaataag ctgtttcgtg tgtggataca tctttttctc ctgcttttct atcagattct
     8341 tacagtccaa gcttaaagct taatctatga ggaatcaacc ttttttacac aaaaaaactg
     8401 cgcgaaaaac cgcgcagttt cccccggtat actattttct ctttctatct ctagaataag
     8461 cgatagtatg accgttcata aaatgacgag acatgcgaca atcgttaaga aagaaaaagt
     8521 taagcgtcgc atcgaatgtc agttttattc ttttcttttg gctcactgac gaaccaccat
     8581 tcgattccta tagcatcata gattgtgagt actgtgtgct ttttgtctaa gaagaaatca
     8641 actgatagtt gctgcaagtg agcaatcagt ccttccatgc tttcagtatc gatcatgaat
     8701 ttcagaaaat caagtccaat cacttcatct gtaggcaaat caattgcgcc tccgctagct
     8761 tctgtcaaac caacatggaa atctccttca ttcaatacat ggatgccctc ttcttcatta
     8821 tgtaccttca aaccaagtac atgattaaga aattggcgtt cttttgttaa gttagtaaca
     8881 tttaaatgga ttttttcaaa ataagaacct gttgatagtt tactgaattt ttcttcagat
     8941 tttcttaata atgcttcttg atccattgtt tctggaattt ctctttggtc gttcttattc
     9001 tcttcatggt aaatttccaa ttcattctct tctgggtcta cgagcaaaat ccccatacgt
     9061 ccatgatctt ctaaagcttc tttgattgga taattcattt tacggatacg acaaaggata
     9121 tctgccattt cttctgtgct agggataatc aatgaaaaac ggttcagctt tttgatcacc
     9181 cccatgtgat cattcgctct aggactttct tccagcagca acatttcact tccagcttct
     9241 tctgttccta aaatggctaa ttcgttttct tctcttttca aatcaaagcc aacaacgttc
     9301 ttgtaaaaat caatcatttt atctcggtct ttcacccgaa tggctacact ttttaaatag
     9361 gtggcagccc ccaattgaaa atctttcata tttcttgctc ccctctacgt caagctttat
     9421 tttaacgatg gattcttctt ctttcaagca ataactttga ttttgttaaa aaaaatgtga
     9481 gaaaattcgc attttgcttg ttttttgtaa attaatgagt aaaaactctt ataaaaagag
     9541 gttgtgaaaa agctgttctg catcaagtaa taagaacagc caaacaaaaa tagctcctcc
     9601 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
     9661 agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt
     9721 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttac
     9781 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
     9841 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
     9901 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
     9961 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
    10021 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
    10081 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
    10141 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
    10201 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
    10261 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
    10321 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
    10381 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
    10441 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
    10501 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
    10561 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
    10621 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
    10681 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
    10741 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
    10801 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
    10861 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
    10921 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
    10981 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
    11041 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
    11101 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
    11161 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
    11221 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
    11281 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
    11341 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
    11401 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
    11461 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
    11521 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
    11581 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
    11641 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
    11701 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
    11761 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
    11821 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
    11881 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
    11941 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
    12001 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
    12061 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
    12121 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
    12181 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
    12241 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
    12301 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
    12361 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
    12421 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
    12481 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
    12541 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
    12601 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
    12661 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
    12721 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
    12781 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
    12841 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
    12901 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
    12961 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
    13021 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
    13081 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
    13141 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
    13201 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
    13261 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
    13321 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
    13381 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
    13441 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
    13501 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
    13561 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
    13621 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
    13681 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
    13741 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
    13801 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
    13861 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
    13921 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
    13981 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
    14041 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
    14101 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
    14161 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
    14221 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
    14281 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
    14341 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
    14401 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
    14461 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
    14521 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
    14581 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
    14641 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
    14701 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
    14761 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
    14821 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
    14881 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
    14941 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
    15001 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
    15061 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
    15121 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
    15181 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
    15241 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
    15301 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
    15361 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
    15421 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
    15481 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
    15541 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
    15601 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
    15661 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
    15721 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
    15781 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
    15841 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
    15901 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
    15961 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
    16021 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
    16081 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
    16141 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
    16201 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
    16261 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
    16321 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
    16381 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
    16441 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
    16501 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
    16561 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
    16621 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
    16681 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
    16741 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
    16801 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
    16861 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
    16921 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
    16981 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
    17041 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
    17101 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
    17161 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
    17221 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
    17281 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
    17341 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
    17401 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
    17461 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
    17521 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
    17581 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
    17641 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
    17701 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
    17761 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
    17821 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
    17881 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
    17941 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
    18001 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
    18061 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
    18121 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
    18181 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
    18241 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
    18301 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
    18361 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
    18421 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
    18481 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
    18541 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
    18601 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
    18661 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
    18721 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
    18781 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
    18841 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
    18901 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
    18961 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
    19021 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
    19081 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
    19141 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
    19201 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
    19261 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
    19321 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
    19381 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
    19441 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
    19501 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
    19561 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
    19621 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
    19681 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
    19741 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    19801 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    19861 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    19921 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    19981 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    20041 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    20101 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    20161 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    20221 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    20281 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    20341 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    20401 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    20461 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    20521 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    20581 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    20641 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    20701 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    20761 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    20821 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    20881 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    20941 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    21001 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    21061 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    21121 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    21181 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    21241 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    21301 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    21361 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    21421 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    21481 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    21541 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    21601 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    21661 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    21721 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    21781 gtcgatcccc ctagatgagg caacataagt atacggtcat tttctaagaa ctcttcttct
    21841 ggaaaatcag tcaagtattt agccagatgt tgtttttcca atgcttgcat aacagcatat
    21901 ttatcaacaa tttcacttct tcctacattg ataagtacag catctttttt cattttttct
    21961 atattcgcct gatcgatcaa tccttttgta tcttctgtca acggcagcat gactacgatg
    22021 taatccgagg tagaaagaac tgtttctaaa tctgcttgcc gaacataatc caactgggca
    22081 tcgcggatag aataacccaa gacatccatc cctaagctat agcaacttag cgctactttt
    22141 gtgcctatcg ctccaagacc aagaagaccg atggttttgc cttgcaattc tcgtcccaca
    22201 taagcacttc ttttgttttc tgcctgttct aaaatattag gacctgttag tgtttgtacc
    22261 atacgacttg cttcgattat cggacgggaa gataagagca aacagcacaa aacaagctct
    22321 tttactgcat tagcatttac acctggtgta ttcatgacga tcgtcccgtt ttcagaagct
    22381 ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg aaattgctag caaatctggt
    22441 gtaagccatt catctttcac tttcgtacta cgaataacta ttccttcggc tggcaaatca
    22501 tctattattt tgaaatattc cccttgtaac ttttctatct cttgttctgg aatagcatca
    22561 atcaaatgaa cgtgatacat aaaaccccct ccttactaac aagtatctcg aaatttctgc
    22621 aattagacaa ctgtttacca tttgacttcc tagaagcaaa aagagcagtt agagcagagt
    22681 aggctgagtg ataaaaataa ggctatgaca agttactgcc ataagcctta ttttcgagag
    22741 caaaatattg agggtgagat atcttgataa atggtgttcc gttagctgac attgataata
    22801 agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa tctcttctta tttcttaaaa
    22861 ctaagtggct ttttcacaac ctctttttat ctaataacca ctgtttccaa ctcaactaat
    22921 tttgcccacg tttcaatttg atctgttgtt agatttagcg agacaacggt atgatgaccg
    22981 ccacctgcct gaatccatga atggacacct tcatggaaat tcggtttagt tttccataac
    23041 acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt caaaggcttc tacttcattg
    23101 atcagcagtt tataatgcgt accgaaatct gccatcgata cgactacccc ttctcctgct
    23161 ttcccatcaa atacaagacg cgccggatct tctctatctc ccattgataa aggagaaaca
    23221 acgattcttg gtttcgtcgc agcaagcgtc ggatctactt ccatcatatg ggattccaag
    23281 attgcttctt ggccactagc tagctcgtaa gtataatctt ccatgaatcc tgtatctttc
    23341 cctcgagcca tgattttcag caaccgatca atcgctgcag ttttccagtc gccttcccca
    23401 gcaaaaccgt atccttctgc catcaaacgt tgtacagcta gtccaggtag ttgtttcatt
    23461 ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc ctccttcttc taagaaacgg
    23521 cgaataccga tttcctgctg agcttgtact tttacatgtt cctcccatgt cttttcttca
    23581 taatcaccat agtcgaaggt atacaattcc ttgtactctt caaataattg atcgacttct
    23641 tcgtctgata cgcgatcgat tacttgtaca agatctccga taccaaaata atcaacagtc
    23701 caaccaaatt gaatctgtgc ttccactttg tctccttctg tgacacccac gtttctcatg
    23761 ttgtcaccaa aacgagccac ttttattcca aaactttcat tataggctac tgcaacatcc
    23821 atccaatcag caatctcttt ttggatctca ggtcgtttcc agtagccaac gacgatttta
    23881 ttttgtttat ttaatcgagc attgatgaac ccatattcgc ggtcaccatg agcggattga
    23941 tttaaattca tgaaatccat atcgatcgtt ttccaaggaa tgctttcgtt atattgcgta
    24001 gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc ggatccacat ttttgctggt
    24061 gagaaagtat gcatccaagt gatcacacct gctacttctt ctcgataatt gacctctttc
    24121 atgatttttg tgatcgtatc agcagtaact gctaattctt gaaggcggat tggataaggc
    24181 aattggccat tttcattcag ttcatcgacc atttcttgtg catgtttctt tacttctcgc
    24241 agtgcttctt ccccatataa atgctgtgat cctacaacaa accaaaattc tttttctcca
    24301 atattcaaca taattagcca ctccttttat ttgttttgtc cgtaatacgc attttttccg
    24361 tgttttcgta aataatgttt atctaaaatc cgttgaggca gctcttctga aaatgaattg
    24421 agctgtctgg taaataaatt catcttgcat acttcatcta gtacgactgc gttcattacc
    24481 gcactttgcg catctttccc ccaagtaaat ggaccgtgtc cgtgtaaaag tacccctgga
    24541 attgctaagg gatcaatttt gcgttctttg aatgtttcta tgataacttt tcctgtttca
    24601 tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc gagcacaagg taccgatcca
    24661 taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat ccaatcctgc ttgtgcccag
    24721 atcgtcgccc atgtcgagtg agtatggacg attcctccga tttgaggaaa tgaacgatat
    24781 aaaacagcat gagtaggcgt atccgaagag ggattgagtt taccttcaat gatatgattg
    24841 tccaagtcca caacgaccat gtcgtctgct gtcatctctt cataactgac tccgctaggt
    24901 ttgataacga aatatcgtgt ttcaggatca aaagcgctca catttcccca tgtatatttg
    24961 acgagtccgt gtttaggcaa gtcaagattt gcttggtata cttcttcttt cagttgttcc
    25021 agcattttct atcactcctt tttcttgcaa ttgatgaatc gctgtttttt caatgtcgat
    25081 cccctcacgg taacgtctca taaatatctg atagcctgct atttcttctg ctgaaggtgt
    25141 gatggtgatt ccttggtctt gtccaaacac gtcttctgcc aagaagtttt ctaaagactt
    25201 cccatgtcga tccatcatat aggctgctaa taacgcgatt ccccacgctc caccttctcc
    25261 ggctgtgtcc atcactgtaa ctggtgcctc catagcagat gctagaatcc gctgtccaac
    25321 ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga tcaatttgga cattctcaga
    25381 tttcaatata tccattccga ttctcattgc accaaaagca ctggctaaat ggatccgcat
    25441 gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca aataatggcc ttccttcatt
    25501 tactcctgta atgttttcac cggaaaaata accataagaa agtaatcccc cgcaatccgt
    25561 atcgccttct agcgctttgt tgaataatac cgtgaataat ttatctctgc ttatctccat
    25621 acccaaagaa ttggtaaatt cttcaaaaat actgacccaa gcattgatat ccgaagagca
    25681 gttattcgta tgaaccatgc ctactagttc tcctgaaggt gtagtgacca tgtcgatttc
    25741 cggatggaca ttttctagtt ctttttctaa tacgatcata gcgaaggcgg aagtaccagc
    25801 cgaaatgttc cctgtccctt tggctacact gttcgtagcg accataccgg ttcctgcatc
    25861 cccttctggc ggacaaaccg ggattcctgg tctcaaattt cccgtagggt cgatcagata
    25921 agcccctgat tcgcttaact tgccagcttc ttctcctgct actagaactt taggcaaaat
    25981 gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac tgaccaaaaa tatttttcat
    26041 ttcttgatgg taggttttcg ttgaaggatc gataggaaac atgcctgatg catctcctac
    26101 gcctagtact ttctcaccag taagctgcca atggacataa cctgcaagcg ttgtcaaaaa
    26161 attaatttct ttcacatgtt cttctttatt caaaatagat tgatacaaat gagcgatact
    26221 ccatctctgc ggaatattgt attgaaattt ttccgttaat attcgttctg cttcttcagt
    26281 gattgtattt cgccaagtac gaaatggtac tagtaactgt tccttgctat caaatgctag
    26341 ataaccgtgc atcattgcac tgaacccaat cgacccgatt gtcgtaagat tctcgccata
    26401 gatttcgaat acttctgctg ccatttttcg gtaactgacg cgcagtcctt tccaaatatc
    26461 ttctaatgaa taggtccaga taccattttc tagtttgttt tcccactcat aactaccagc
    26521 ggcaatagga gtaaaagatg aatcaatcaa tacagctttg atcctagttg atccaaactc
    26581 aatccctaaa gatgtacgac catctatgac atctaacata ctttgcgcat ttacttcctt
    26641 cataatgatt taactccctc cttctcgtca aacctgatca aaccgtcttt ttagtagcgc
    26701 ttacaatgat tattgtatac ttttgtacgt acatgtcaag cagttattta caagaataca
    26761 ataaaaaaca cttgttttgt acgtacataa gttataaaat gaaattgctt acaaaatctg
    26821 atcaaccgcc ttctcgaaat tctagaagga gagaatactt atgaaaaaag aaaaaaaagt
    26881 ttcctcatcg tttatttatt tttttggcgc atttggcggc attctatttg gatatgatat
    26941 tggtgttatg accggcgcct tacctttttt acagcatgat tggggtttgg caggtaaagc
    27001 ttctttgata ggatggatca cgtcttcggt catgttaggt gcgatccttg gcggctcttt
    27061 atctggtctt ctttctgata agcttggacg acgaaaaatg attcttcttt ctgcacttat
    27121 ttttatggct ggttctgtcc tttctgcatc tgcccctcac aatggttctt attttctgat
    27181 tgcagcaagg attcttctag gtttagctgt tggcgctgca tctgccctag ttcctgctta
    27241 tatgtctgaa atggctcctg ctagactaag gggaagattg tcagggatca atcaagtgat
    27301 gattgcttct ggcatgctcc tttcctatgt tgcagattat ttgctaaaag ggttgcctga
    27361 aacgatggct tggcgcgtga tgttagggct cgctgctgtc cccgcgctga ttttattttt
    27421 cggtgtttta gctctaccag agtcaccgcg ttttcttatg caatctggcc gcttggaaga
    27481 ggcaaaaaga gtattgaact atattcggac accaaaagaa gctgaacagg aattcgaaca
    27541 aatccagctg aatgtcaaac aagaaaagac aaccggaact tcttggcata ctttgttttt
    27601 agaaaaatat cgttccttag tcattgccgg tattggtgta gcagtattcc agcaatttca
    27661 aggagctaat gctatttttt actatattcc gctgatcgta gaaaaagcga ctgggcatgc
    27721 agcgagcgat gcattgatgt ggccgatcat ccaaggaatc attttagttg ctggttcttt
    27781 gctctttttg gttattgcag acaagttcaa tcggcgaact ttattgaaaa taggcggtag
    27841 cgtgatggga ctttctttca tcctaccagc tgttttagga actgtactgg atgctcatac
    27901 aaattcgttg ttgattctct tgtttttatg tatttatgtc gcattttatt cttgcacatg
    27961 ggctccactg acatgggtaa tcgtaggcga aatattccca ctagctgtcc gcgggcgagc
    28021 atctggccta gcctcttctt ttaattggat tggttctttc ctagttggct tgctttttcc
    28081 tgtgatgact gcttctatgt ctcaagaaat cgttttcggc atttttggga tcatctgctt
    28141 tttaggggtt ttatttattc aagagatcgt accagaaact cgtgggaaat ctttagagga
    28201 aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt atttctatcc atcattctta
    28261 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaaccgct tagtttccag
    28321 tcacaaggaa caatttcaaa aaacagcttc tcatgtttga tgaatattgt gacttgtgac
    28381 cagttggtag gagaacaccg tttagtggag aggtggtgaa agaaacgttc agccttgagc
    28441 gatgacaaaa gctctcacaa acattgcgac gagtaaccgt aggagcaata agaggcggta
    28501 aaaacccgtc tcttattttt cgttttcttt cactgtttcc cgttcaacta gtacaggttc
    28561 atagagaata tttggctgtg cctgtccttt ttcaatcgtt gtgacgatcc accgtgcggc
    28621 atcttgtccc aacttttctt ttggatgcgt caatgttgtc aattttttat tccctaaatg
    28681 gcttaaagaa gaatcgtctg ttcctacgat cgataggtca tctggaatat gatagccatt
    28741 ttctgtcaac ttatctagca ttaaactagc tatttgatcg ttatagcaaa tgatgccatc
    28801 taaagcttgt tcttctttca accgttgtac tgttttttca gcgattgttt ctcgtgtttc
    28861 cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca ttatactgtt cacacgcgct
    28921 gataaaacct ttcatcctgt attttccttg aagatcgtcg atttttgtga tgaacaacag
    28981 acgctgatgt ccttttttta tcaaatattc tgctgccata aaaccgactt tcacatcatc
    29041 taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca ccatcggaat
    29101 gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat tacttttcgt
    29161 aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac actctttctc
    29221 caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt ctcgtaattc
    29281 attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg tggtgatgac
    29341 accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt catctacata
    29401 tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag cgattgcttg
    29461 ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg agggaatggt
    29521 tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg caatatcttg
    29581 atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat aacgagtctg
    29641 caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac ggaacaggtt
    29701 caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat
    29761 taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga tagatcaaaa
    29821 tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca aattgcttat
    29881 tttttttaga agaaacgaat actggctccg ataatttatg ccatgattct tttaataaaa
    29941 gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct gttgcactac
    30001 cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct tcatttactg
    30061 aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc ttccccttta
    30121 acgtccaagg attatccatt tcggatagat aaagattaga attcccagta atttcaggat
    30181 cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt ccgtctaaac
    30241 tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta ttcattggat
    30301 ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca cgtatagttt
    30361 tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg acttcaggtg
    30421 cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc aggcttgcag
    30481 catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg tagcctggaa
    30541 ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga tctgcccgtt
    30601 gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc acattttctt
    30661 tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt catgtttttc
    30721 tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg tatttttgtc
    30781 caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat tgaaatcatg
    30841 ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag tcagctgatt
    30901 gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt cacaatcata
    30961 aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg ataagtgttg
    31021 catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca gtattaaagg
    31081 tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt gtcgtaaaat
    31141 agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac accaatcgat
    31201 gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta cattccattc
    31261 atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat aatcacaagt
    31321 agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt
    31381 atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact tatcaatata
    31441 gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga tacgatccca
    31501 ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt caattgaatc
    31561 atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt cataagcact
    31621 cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata atttaacgcc
    31681 aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc cttttggaaa
    31741 attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta aattcaccgt
    31801 gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga gtccaaaaga
    31861 atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt ctttcctacc
    31921 tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc gaatgacacc
    31981 taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac catcagctgt
    32041 ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct caacaaatga
    32101 gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt ttactttcac
    32161 ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa tagatacaag
    32221 tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata tgagcagtac
    32281 aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa atactttcca
    32341 tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa tcacatatat
    32401 ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa ttgaaagtac
    32461 gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg ttaacagact
    32521 aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat aaatgatatt
    32581 tcctgatccc tcacctttcc attgcgccat tacataaaaa acagcacatg tagcaggcac
    32641 gatagtaata acaggcaaac acacgattac ccagagaata cctaataaga tgtaatcaga
    32701 aaccacaatc aactgtccat aaagtttagt ctcaactatt ttcatcttcc tcactctttc
    32761 attccagaca tagctgttcc ttgaacaaaa tatttctgtg caaaaatata aaccactagt
    32821 aatggcagca ttgaaataac cgttccagcc attacaggtc cataaaaaga aatgttctgc
    32881 cctgtaaata aagctaaccc tgctggtaaa gttctcattt ttgaactact tgtcaatatc
    32941 aatggataaa gcaagtcatt ccaactattc atcagcgtga aaatccctaa tgataaaaga
    33001 gtaggtttgc tcaatggcaa catgattttc aaaaaaatat taaactcact taatccatca
    33061 atgcgtgctg cctcttccaa agaatctggt aaagtaataa aaaaggaacg catcatgaag
    33121 ataccaaatg cagttgtcat acgaggaatg atcaaacctg cataagtatc aagcatgcca
    33181 agcaaattga cttcaataaa tagaggaatc atgaagactt ggaatgggat catcattgtc
    33241 agcaaaacga aatagaacaa aactgatttt cctttgaaac gcatacgagc aaatgcatac
    33301 cccgcaagag aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa aatcactgta
    33361 tttttgatgt aatctaataa agggatcgtt gtccaaacct taatatagtt atctagggta
    33421 tagctatctg caaatattct aggcggaaat gagataatgt ctttttcgtg tttaaaagaa
    33481 gaaataatca gccacaaaaa aggaaacgca ataaccaaca atataactag catactaatc
    33541 gttcgaatga tttttttacc actaactgct tttcgactat tcacagtaac tgtaatcgag
    33601 ctttctggtg ccatacaggt ttccctccta tttcatttgt ttttctttcc gtgacatgta
    33661 cacattagtg gagatagcta tagttgcaac aatgataaac aaaaccgaag acagtgctga
    33721 tgcatagccc aattcataag gtgctgtaaa tcctcgactg taaatatatt gaacagctgt
    33781 ttctgtctta aattgtgggc caccagcggt tgcaacatat acttggtcaa acacttgcat
    33841 cgaaccaatc agattcgtta aaatacaaaa acctaaggag ggaacgatct gaggtagcgt
    33901 aatgttaaaa aactgctgtc gatttgttgc accatccatt tgagcagatt catacaaaga
    33961 gggagagatt ccctgaatag cagtcaagag aatcgtcata gtaacaccaa aattcttcca
    34021 aacagtcatg atggctaccg tcggcatagc aagggttgga tcgcgaaaaa attgagggat
    34081 cggcaagccg acttgtgaaa acaagtaagg aatcgcccct acattcggat caagcaacat
    34141 agaaaaaata atcgcaattg acgtcaatga acaaataact ggaatgtaaa atgtcgaacg
    34201 acaaaaacga ttgaatctgg tatttttaga taatgctact gctgctaata aaccaacgat
    34261 aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg aattaactgc
    34321 tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc ttgatgtaaa
    34381 gaaaatattc aaatctgtaa aactaatagc aaatgttcct aaaagcggta tgacagtaaa
    34441 taataataaa actaaaaaag atggagttaa aaacaaatac gcagcacgat caggacgatt
    34501 ccaatatttg gtgaatccgt tcatatagtc cacctcatct ttcattttga taaaatatac
    34561 aatcttggtt taaacgaatc tgaataagat atccgaacaa tcgctgtgat ttttgcaata
    34621 acacctatct gcaaatacta ggtcgatcat tcagatatct gtatcttgcc aacagtctat
    34681 cctgtataaa gttctgtact atttttgcag taattgattg atggcatcat ctgcatcgtt
    34741 catgagtgtc tcaggatctt ctcctgctaa taatttttca atcagtggat tgatgatatc
    34801 gttattgatc gcagagattt gggtaaaacc tggtaaatat ggctctgcat agtccatttg
    34861 tttcgataat tcagatacaa taggatcatt ttttacttct tcatcttttg ctactgaatc
    34921 caagtaggca ggaaaaccat tttcgatact ccatttttta ccaatttcag ttgtattcca
    34981 atatttcaaa aactcataga cagcttcttt tttattatta tctgtacttg ccggaatggc
    35041 atatcctacg ccatctaaaa tagctgtttt tttgtctgaa tcactccctt gtggtaaggt
    35101 agtcacatta taatcaatct cgtttgcttt caatccgtta tttaaccatg gcccattgat
    35161 ttcaatggct agttgaccag cattcattaa attgtccatt tcggcgccag aaatcgattc
    35221 tggaccagtc ttgtctacat ggatcatttt ttggatcgct tttaacgttt taaggttctc
    35281 aggagacgca aattcagact tagtataatc atcgttcact aatttaccgc catttgctaa
    35341 catccagtta tataacggtg cagttccatc cttattgaag acaaaacctg atacattttt
    35401 actactatca gttaattttg gtgccatttc tgtcaattct tcccaagtag taggtggttg
    35461 ttcagtatct aatccagctt gtttaaacaa atcattattc caatacatat acatgccttg
    35521 tacctgcatg gggacaaaat actgtttacc ttctacttca cctaattcaa tagccgtttc
    35581 tttaaaatca gatttatcta ctccatcata ttcccagaaa tcgtctaatg gttgtaccgc
    35641 tccgttttct acatactgtg caaaatctgg ataatttaat gcaataaagt caggtgcttt
    35701 tttagaagaa atcgctgtag gaagtttttc attcaaattg gcccaagtca tgatatccat
    35761 ttcaattttg atgtttttat catttgtttt attaaattca ttaacgattt cttgcaatat
    35821 ttcaccatca gatgcagtaa acccattcca aaaagtcaaa gtcacttctt tatcagaatc
    35881 agattctgct gaacttccgc caccgcaagc tcctaaaata cctgttgcag ctaccaacaa
    35941 agagccaatt aaccattttt tacctttcat tttttgttcc tccttcatct gataaacgac
    36001 atctgaccac attccaagat agcggtttca ataaacaatt gattttccca tcttcagctt
    36061 ttacatttgt tgaatgactt ggttttactt gttcactttt aggaagatta gttgcttttg
    36121 tatcaaatcc actcatttca gaaaaatcaa tcactccctc taatgataat tcagacaagt
    36181 gaacagttag attcatttct tcttctccac ggttaactgc aaatatgaca agttctttgt
    36241 catttattac ggctatactt tctaaatacg gcacttcttc aaaatcttcc gtcgcatagt
    36301 ttgctgaacg aatttgtggg cttaacacct ctccttgtcc atacacagca gcttgcataa
    36361 atggataata gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg attggtgcaa
    36421 taacatttac caattgagca agacaagcaa tctttactct atctgcatgt ttcaataaag
    36481 tgattaatag acagcctaat agtaatgcat cttcaaaatt atagttatct tctagtaaag
    36541 gaggagcaat ctgccaaggc gctgcttgct catcttgttc cgctgaatga taccaaatat
    36601 tccattcatc aaaggacagg ttgatctgct tcttactatg tttcttcgcc ttgactgtat
    36661 cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc taaggaacgt gcaagataat
    36721 gagaaagatc atttttttgg ttgccataat attgatgcag agacaaataa tctatttgat
    36781 cgtacgcttg atcaagaact gttaactccc aatcaccaaa tgtaggcatt tgccgattcg
    36841 aacttccgca taaaaccacc tcaattgaat catctacgag tttcatcgcc ttagccgttt
    36901 cctctgctaa ccgtccatat tcttccgctg ttttatgtcc aatctgccaa ggaccgtcca
    36961 tttcattccc taaacaccat gtcttaattc caaaagggct ctcttgacca ttcgaacgcc
    37021 ttaaatcact ccaataagtt cctttaggaa aattacaata ttcgactaaa ttacgagctg
    37081 catctactcc acgagtaccc agattgacag ccatattgat ttcggccccc acatgctgac
    37141 accagttata aaattcatga atcccaactt cattcgtttc aattgtccgc caagctaaat
    37201 ccaaccttct aggtctattt gctatgggcc caatactgtc ttcccaacga aatcctgaca
    37261 caaaatttcc accaggatat ctaatcatgg gaatgttcaa ttcttttacc aagtgaatga
    37321 cgtcttggcg aaatccactt ttattagctg atggatgatc tggctgataa atcccttcat
    37381 atactgctcg tcccatatgt tcaataaatg aactgtacaa ccgcggatca actttgccta
    37441 cacgcttgac actactaatc aacattgtgg cttccataac tgacctcctt taatttatac
    37501 atgaataata cacctcgtt
//
LOCUS       DACCXW010000010        35962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCXW010000010 DACCXW010000000
VERSION     DACCXW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.010"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            10..1251
                     /locus_tag="IVY71_000429"
     CDS             10..1251
                     /locus_tag="IVY71_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7984504.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            1360..2409
                     /gene="recA"
                     /locus_tag="IVY71_000430"
     CDS             1360..2409
                     /gene="recA"
                     /locus_tag="IVY71_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7984505.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            2820..4376
                     /gene="rny"
                     /locus_tag="IVY71_000431"
     CDS             2820..4376
                     /gene="rny"
                     /locus_tag="IVY71_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7984506.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(4455..5261)
                     /gene="proC"
                     /locus_tag="IVY71_000432"
     CDS             complement(4455..5261)
                     /gene="proC"
                     /locus_tag="IVY71_000432"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7984507.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            5402..7975
                     /gene="mprF"
                     /locus_tag="IVY71_000433"
     CDS             5402..7975
                     /gene="mprF"
                     /locus_tag="IVY71_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7984508.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            8129..8980
                     /locus_tag="IVY71_000434"
     CDS             8129..8980
                     /locus_tag="IVY71_000434"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7984509.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            9225..10163
                     /locus_tag="IVY71_000435"
     CDS             9225..10163
                     /locus_tag="IVY71_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7984510.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            10186..10866
                     /locus_tag="IVY71_000436"
     CDS             10186..10866
                     /locus_tag="IVY71_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7984511.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            10839..11663
                     /locus_tag="IVY71_000437"
     CDS             10839..11663
                     /locus_tag="IVY71_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7984512.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            11830..12648
                     /gene="purR"
                     /locus_tag="IVY71_000438"
     CDS             11830..12648
                     /gene="purR"
                     /locus_tag="IVY71_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7984513.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            13151..14524
                     /gene="glmU"
                     /locus_tag="IVY71_000439"
     CDS             13151..14524
                     /gene="glmU"
                     /locus_tag="IVY71_000439"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7984514.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            14618..15589
                     /locus_tag="IVY71_000440"
     CDS             14618..15589
                     /locus_tag="IVY71_000440"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7984515.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      15595..15697
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15763..16332
                     /gene="thiT"
                     /locus_tag="IVY71_000441"
     CDS             15763..16332
                     /gene="thiT"
                     /locus_tag="IVY71_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7984516.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(16413..17186)
                     /locus_tag="IVY71_000442"
     CDS             complement(16413..17186)
                     /locus_tag="IVY71_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984517.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            17453..19153
                     /locus_tag="IVY71_000443"
     CDS             17453..19153
                     /locus_tag="IVY71_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP7984518.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            19123..19341
                     /locus_tag="IVY71_000444"
     CDS             19123..19341
                     /locus_tag="IVY71_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984519.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            19361..20251
                     /locus_tag="IVY71_000445"
     CDS             19361..20251
                     /locus_tag="IVY71_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7984520.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            20269..20553
                     /locus_tag="IVY71_000446"
     CDS             20269..20553
                     /locus_tag="IVY71_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984521.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            21040..21654
                     /gene="gmk"
                     /locus_tag="IVY71_000447"
     CDS             21040..21654
                     /gene="gmk"
                     /locus_tag="IVY71_000447"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7984522.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            21659..21964
                     /locus_tag="IVY71_000448"
     CDS             21659..21964
                     /locus_tag="IVY71_000448"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7984523.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            22217..24625
                     /gene="priA"
                     /locus_tag="IVY71_000449"
     CDS             22217..24625
                     /gene="priA"
                     /locus_tag="IVY71_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7984524.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            24640..25131
                     /gene="def"
                     /locus_tag="IVY71_000450"
     CDS             24640..25131
                     /gene="def"
                     /locus_tag="IVY71_000450"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7984525.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            25124..26062
                     /locus_tag="IVY71_000451"
     CDS             25124..26062
                     /locus_tag="IVY71_000451"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7984526.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            26062..27420
                     /gene="rsmB"
                     /locus_tag="IVY71_000452"
     CDS             26062..27420
                     /gene="rsmB"
                     /locus_tag="IVY71_000452"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7984527.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            27433..28173
                     /locus_tag="IVY71_000453"
     CDS             27433..28173
                     /locus_tag="IVY71_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7984528.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            28170..30239
                     /gene="pknB"
                     /locus_tag="IVY71_000454"
     CDS             28170..30239
                     /gene="pknB"
                     /locus_tag="IVY71_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7984529.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            30404..31303
                     /gene="rsgA"
                     /locus_tag="IVY71_000455"
     CDS             30404..31303
                     /gene="rsgA"
                     /locus_tag="IVY71_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7984530.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            31316..31966
                     /locus_tag="IVY71_000456"
     CDS             31316..31966
                     /locus_tag="IVY71_000456"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7984531.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            31967..32605
                     /locus_tag="IVY71_000457"
     CDS             31967..32605
                     /locus_tag="IVY71_000457"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7984532.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            32760..33614
                     /gene="mreC"
                     /locus_tag="IVY71_000458"
     CDS             32760..33614
                     /gene="mreC"
                     /locus_tag="IVY71_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7984533.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            33618..34127
                     /gene="mreD"
                     /locus_tag="IVY71_000459"
     CDS             33618..34127
                     /gene="mreD"
                     /locus_tag="IVY71_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7984534.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            34382..35956
                     /locus_tag="IVY71_000460"
     CDS             34382..35956
                     /locus_tag="IVY71_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984535.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        11862 a         6430 c         7809 g         9861 t
ORIGIN      
        1 cgggaagtaa tgaaagcaga aataatagct gtcggaacag aacttttact aggacaagtc
       61 gtgaatacga atgccacttt cttgtcagaa cagctagctg atctaggtat cgaagtttat
      121 tatcaaacag ttgtaggaga taatcagcag cgtttagaag agttgattgc tctagctgaa
      181 acaagaagtg aactcatact cttatgtggc ggattaggac cgacagaaga tgacctgaca
      241 aaagaagcaa ctgcggctca cctcggtaag tctctcatac aaaatacgga aggatacaaa
      301 aaattattgg catattttga aacaacccat cgaaaaatga ccaaaaacaa tctgcaacag
      361 tcccagatta tcgaaggcgg ggttcctcta ccaaatcgta cagggcttgc tttagggact
      421 ttttatcaaa cagatacaca tgcgtatatc cttcttcccg gtccgcctaa tgagttgaaa
      481 ccaatgtttg tggaacaagt acgtccttta ctagaggaac gttttccttc tgaagaaaaa
      541 ctaatttcta aagttttgcg cttttatggg attggtgaat cgcgactggt cactgagcta
      601 aaagatctga ttgagacaca gatcaatcca acgatcgcac catatgccaa accaaatgaa
      661 gtgacattac gactgacagt gaaaacaaat gatgttcagg cagggaatca agctctttta
      721 gcattagaag aaaaaatcca agaacgagta ggggaatatt tttatggata cggcgatgat
      781 aattcgctag caaaagtcgt agtggaacta ctgaaagaaa acaagcagac tgtaacagca
      841 gcggaaagtt tgacagccgg cgccttccaa gccgcattag gcgacattgc tggtgtatct
      901 gaagtatttc caggcggatt cgttacctac tcgctccaaa caaaagctgg ttttctggag
      961 attgatccgg agctattagc agaatatgga acagtcagca aagaatgtgt ggagcagatg
     1021 gccattcaag cacgtgagat agcaagaaca gattacagtg tagctttcac cggagtggct
     1081 ggaccggaca cgttagaagg tcaatctgca ggaacagtat ggatcgctat ttcatctaaa
     1141 gacggcgtgg tttcggaaaa atatcacttt gccagagatc gtgctgctat cagatataat
     1201 gcggttatgc aaggatttga tcttttgaga aaacagcttt taagaaaatg ataaatgcga
     1261 atatttgttc ggttttttct tgcttttttt cactaaaaaa gatatgataa ggataatgaa
     1321 atgttgctct gatttgtaaa tataaggagg attatcgtat tggcagatga tcgtaaagca
     1381 gccttagatg ctgcactgaa aaaaattgaa aagagctatg gtaaaggctc tattatgaaa
     1441 ttgggcgaaa aaatcgacca acaaatctct acaattccaa gtggctctct tgcgttagat
     1501 gtcgctttag gtgtcggcgg ttacccccgt ggacgtatca tcgaagtata tggacctgaa
     1561 agttcaggta aaacaacagt tgcactacac gctattgcag aagtacaaaa aaatggcgga
     1621 acggccgctt tcattgatgc tgagcatgcg ttagatccgc aatatgcaca aaaattaggt
     1681 gtgaatatcg atgaactact tctttcacag cctgacacag gagaacaagg tctagagatc
     1741 gctgatgctt tagtatcaag tggggctgta gatatcgtag tagtcgattc agttgctgct
     1801 ttagttccac gagcagaaat cgacggcgaa atgggtgact cacatgtcgg gttacaagca
     1861 cgtttgatgt ctcaagcatt gcgtaaactc tctggttcga tcaacaaaac aaaaacaatc
     1921 gctattttca tcaaccaaat ccgtgaaaaa gtaggcgtga tgtttgggaa tccagaaatc
     1981 actcctggag gacgcgcatt gaaattctac gcaacgatcc gtttggaagt acgtcgtgca
     2041 gaacaattga aacaaggtac agatattgtt ggtaaccgta caaagatcaa agtcgtaaaa
     2101 aacaaagtag cacctccttt caaaatcgct gaagtagatg tcatgtatgg tttagggatc
     2161 tcacaagaag gagaacttct agacatggca gtagaaaaag acattgttga taaaagcggt
     2221 gcttggtatt cttataaaga agatcggatt ggtcaaggac gggaaaacgc taaaatctat
     2281 atggctaatc atcctgaaat gatggcagaa gtttctgctc tagttagagc agcttatggt
     2341 attggtgaag aagtcgctgt cccagaagat gaaaaaggac aagaagaatt gcctttagta
     2401 gaagaataaa aattatgata aacaaaaacg gctggtctga agagtaggta ctctccttga
     2461 cctgccgttt ttttattgaa taaagaaatc gtgagttatt taattatacg agtgggtaca
     2521 taaaataaaa atatctgttc atctacaaag aataatttaa tagaaaagtt tctcaattta
     2581 aaggtttacc tatatgaaac tagtgtcatt tattttaaaa accatcgttt aatgaataag
     2641 tagaaagccg tttctttgtt tatttaaagt tgacactacg gggtacaaac attagaataa
     2701 agttgtgcat gttttttgta cacatatatg cgaagatgca tattgacgag tttagaataa
     2761 tttatcatag aagctgtata gatgataaaa atgaaaaaga tagtatggag gtggatttta
     2821 tggtattcaa cattctcctc gctatcatcg gtttaattgt cggtcttggt ttagggttta
     2881 tggtcgcaaa gtcacgtcac gagaaagaaa tagctggtgc acaatcatca gctgctggaa
     2941 tcatcgatag tgcaaaaaaa gaagcagaaa ctctaaaaaa agaagcattg ttagaagcta
     3001 aggaagaaaa tcagaaatac cgttctgaag ttgaaagtga gctgagagaa agtaagttag
     3061 aattaaaatc tcaagagaat cgtttgattc aacgcgaaca aacgctgaat cgtaaagatg
     3121 actcgttaga aaaacgcgag aactcacttg aaagtaaaga agaagcactt agttcaaaac
     3181 agcaattaat tgaagagcga gaaaaagacg ttgaaaaaat gattgaagat caacaaaaag
     3241 aactagaaaa aattgctggt ttatcaaaag aggacgcacg agatgtcatc atgaaatcga
     3301 ctgaagaaga gctgaatcat gagttaactc taatggtaaa agaatctgaa cagcgtgcca
     3361 aagaagaagc agatcgcaaa gcgaaaaact tattatcatt agcgattcaa cgctgcgcag
     3421 cagatcaagt ttcagaaacg actgtttcag tcgttaccct tccgaatgat gaaatgaaag
     3481 gtcgtatcat tggacgagaa ggtcgtaata ttcgaacgct tgagacgctg actggtatcg
     3541 atttaatcat cgatgataca ccggaagcag ttgttctttc cggattcgat ccaatccgaa
     3601 gagaaatcgc acggatgacc ttggaaaaac tgatacaaga tggacgaatc catccagcgc
     3661 gtatcgaaga aatggtcgag aaatctcgta aagaaatgga tgaacgtatt cgcgaatacg
     3721 gggaagaggc agcgtttgaa gtcggtgctc atacacttca tccagatcta atcaagatca
     3781 tcggacgtct gcgattcaga actagttatg gacaaaacgt cttgaagcat tcggtcgagg
     3841 ttgcaaaatt agctggtatc ttagcagcag agcttggcga agatatccaa ttagcaaaac
     3901 gtgccgggtt gctccatgat attgggaaag cacttgacca tgagattgaa ggatctcacg
     3961 ttgagatcgg tgcggaatta gctgccaagt acaaagaaaa cccagtagta atcaacgcga
     4021 tcgcttctca tcatggagat gtggaagcta cttctgttat ctctgtcctt gtagctgcag
     4081 cagatgcgct ctcagcagca agacctggtg cgcgaagtga atcattagaa aactacatcc
     4141 gtcgtttaag aagtctggaa aatatttcga acagcttcgc cggtgtagaa tcaagttacg
     4201 cagttcaagc tggacgcgaa gttcgggtaa tggtcaagcc agaagaaatc tcggatttag
     4261 acgctgttcg tttagtacgt gatattcgta aacgaatcga agaagaactg gattatcctg
     4321 gacacatcaa agtaatcgtt attcgggaaa ctcgtgcaac tgattatgct aaataataaa
     4381 aaatacaaaa gaagagccgc ggcaaaagcc acagctcttc tttttttgta aggcacgaaa
     4441 aaacattgct atcattattt tttattcaac atctcaagat ctttctcgat cgtcgtattg
     4501 attcctttga tgattgctgg gatgaatcct gcttcttcta aggcaactac acctgcaaca
     4561 gttgtaccgc caggagaaga aacttggtct acaagttccc atggattttt atcacttgaa
     4621 aggaattttt gacaagaacc cataattgct tttgcagcaa tctcagtggc tgtctgtttg
     4681 gacaaaccat tcagtacacc agtccgtccc atcgcatcga tcagcatgta aacgaatgcg
     4741 ggtgagctgc cagcaaggcc aatgaatgta ctgaaatctg attctgctaa aggatagacg
     4801 ctgccgattg cttcaaaaat catcgtagct tgctgaagct gttcctccgt ggcagcttca
     4861 tttttgcaaa tcgcagaaac gcctgctcct acgctgacat tgacattcgg catcacacgt
     4921 acgatggagg cctgaggatc ttcaagcgct gtttctaaat ctattaatga ttttcctgaa
     4981 gcaatagaaa tgaataagac tttctggcta tattctctta gttcttccaa tactttttca
     5041 atgatttttg gtttcacggc taaaagaacg atatctgatt gtgatgcaac ttcctgatta
     5101 cttttgcatg catggacgcc agtttcagca gcaaattttt gtgcattttc aaaatgtcca
     5161 ctggaaacga acacctcttc tttcgagact gttccttttt ttaataaacc atcaataata
     5221 gcttgtgcca tattccctgt gccgataaat cctactttca tgttatcctc tcctttagca
     5281 ttaggatatc tttctttttc tatcatcgca agcaaagaac aggtctaaag tggaaaaatt
     5341 aaaataaata agataacatt ggggtatgac acaaaagtat acaagttagg aatgggaaaa
     5401 tgtgaaaaat aaacgatact tgatgctgca atggataaag gaacatagtc tgattttaaa
     5461 actgattttt ttcggctccg tgttagtctt tgtggcaaat caagtcgcaa atattgctaa
     5521 tggcatgagt tggcaagata tttggcaaag gatgggacaa caaagccgca tgacgatcct
     5581 gctcatgatt ctggctggac tgctgggtgt cactccgatg ttgttgtatg attgggtgac
     5641 gatcagagtc ttagaaaaac aaggaaaacc aaaaatggaa agaaaagagt ttcttcttgc
     5701 tgcgtgggta acaaacacga tcaacaatct cgccggtttt ggtggtgtgg taggagcaag
     5761 tctgcgggct agtttttatg gaaagcagac aaatagaaaa atggttttag ccactgtatc
     5821 aaaagtcgca ctatttatgc tgactggttt gtctgtatgg tcgtttctta catttattga
     5881 tgtctttttt attcagtcag aaagtatttt tcgaagctat tggatctggc ttttcggcgg
     5941 tagtttttta gcaccagtca ttttcttttt tgcttatcta aagagaaaga cattgttctt
     6001 cgagttttat ccaaaaggct tgcttggtct gctactggca tcctttggac aatggacggg
     6061 agcccttgcg gtatttttga tcatcggcaa gttgatgaga atagatattt cgatgctttc
     6121 gatctatcct atgttcatga ttgctacatt gataggaatg ctgacgatgg tcccaggtgg
     6181 aatggggact tttgacgttt tgatgatctt aggaatgagt caattgtctg ttcggcaaga
     6241 tgtagcagta gtttggctat tatattatcg gttgttttat tatgttctac cttttatgac
     6301 gggacttcta ttgttcatca cacatacagg agtcaaaatc aatcgatttt tagataacat
     6361 tccgcgactg tttttccaaa aggttgcgca tatcgtgctc gtaagtgctg tctattttgc
     6421 ggggatcatg atggtacttc tatcaacaat cactaatctg tctgcaatca gtcggctatt
     6481 tcaagtatta ttgccgttct cattcaattt tttagatcag acagtgaatc tattggttgg
     6541 ctttttatta ctcggccttg ctagagcact ttcagtaaaa gtcaaaaagg catttctgcc
     6601 tacgattgct ttactatctt ttggtatttt aaatacaatc acacgtacgt tatcttggca
     6661 gttgatattg gtatatgtct tgattctgtt agctgtgtgg ttatccagaa aagaatttta
     6721 tcgaacaaaa ttcgtttatt cttggggagc tataatcttt gatgctagct tgttcggctt
     6781 tttgtttatc gtttacgcaa ttgcaggcta tcacagcgga cgctttttag tcggtcgaat
     6841 aacaggaaat cattttattt tgtttccttc agatgatgtg tggttctcag ggcttattgg
     6901 tttgggcatc tcattgctag ctttagtggc gctttatcaa tatttagctg aagatactac
     6961 gaagcttggc agtgagtacg aaaaagaccg ttttgaccgt ttattggaaa catacggagg
     7021 cacagctaca agccatcagc ttcgcttgtc aggttacagt tatttttatt accaagaaga
     7081 cgaccaagat caagtcgttt ttggttatca gatcaaagga aacaaatgct ttgttttagg
     7141 agatccagtt ggcaatccta aaatgatgcg ggcagcaacg aatcagttta tgaaacaagc
     7201 agattgttta ggctatcagc ttgcgtttta taagatatca gaaagctacg tagtgatgct
     7261 tcatgaattg ggttttcact tcacgaaagt aggtgaagca gggattgttg atctagcaac
     7321 agaagacttg tctcggctga gtcagcaact tgaatatcaa cgtttgatga aagaaggata
     7381 ccattttact tggtacgatc aactgccgga agaattacat caagctgtaa atgaagtttc
     7441 acaaaaatgg cttcaaggtg cacgagaaaa atacttctct gtgggacgat ttgataaatc
     7501 atatgtatta tctagcggtg tcggtattgt ccgtaaagat caaaaagtcg ttggcttcat
     7561 caccgctcag ccgatgacga agaagcaggc gggatatgat ttgttgcggg tcttacctaa
     7621 agaatctgag gaattagctg actatctgtt agccaatctg tttattgtat acgagaaaat
     7681 gggctactcg gaagccaatc taggcttagc tccattagct aatgttggag aaacagattt
     7741 ttctttcttt caagaaaaag tcatgcatat cgtatataat tatggtaatt ttttctattc
     7801 cttccagtct gcttatgaaa agaaattaaa atatgtcgat cattgggaag gacgttattt
     7861 tgcttatagg aaaggaagta atttcggatt tgctaccatg cagctactca gtctgatagg
     7921 aaaagaaaaa ggaaatgttc cgagtttggc agaagaagtg ctcacagaat tataagaatc
     7981 actagaactt gtttggatta tgaagagaaa attctgtgaa agctgttgaa acgatgacaa
     8041 aatcaaccac taggcagaca taagctatac ttttattttt ccctatgata gaatacttgc
     8101 gacttagact tttcgaatgg gtgagaggat ggaattgatt gaaaaagcgc cagccaagat
     8161 aaatttagga ctggatgtat tatatagacg gcaagatggg tatcatgagt tggaaatggt
     8221 gatgtcgagc gtagacttgg cagatcatct attttttgaa gaaataacag aagacaaaat
     8281 cattgtagaa acaaataaag catttttacc ggtagatgaa cggaacaatg tttatcaagc
     8341 tgctttattg ctaaaaaata aatacaatat caaaaccggt gtcaagatct ttgttaagaa
     8401 gaatattcca gtagcagctg gattaggcgg aggaagtaca gactgtgcag ctgctctaag
     8461 agggatcaac caattatgga atctgggctg ttcgttagaa gaactggcag aaatcggttt
     8521 ggaagtcggt acagatgttc cttactgtgt ctacggaagt acggcatttg tcggtggcaa
     8581 aggggaagaa atccaagtat taccttctat gccacagtgc tgggttgtgt tggtcaaacc
     8641 aagaatgagt gtctctacac gtacagtgtt tagagaagta gatacgcaag ccatccaaca
     8701 tcaagacatc cacggacttg ctgaagccat ccgagagcaa gattatcagc agatgatcaa
     8761 aaaaatggga aacagtttag aagatataac gggaaagcgg catccagtca tccatcaaat
     8821 caaagagcgt atgatcaagt acggagcaga tgctgcattg atgagtggaa gcggaccaac
     8881 cgtttttgcc ttgtgtcaac attattcaag agcgcagaga gtatataatg ggttaaaagg
     8941 tttttgtgag gaagtttatt tagtacgaac cctgcaataa aatataagaa gtaaaatagg
     9001 tgatgaaaaa ctgaggaatc ctaaaatggt attcttcagt tttttattgt agtgaaatgg
     9061 ctagatatga gcagcaactc atccatttta cgatttaatc aagaatatac atgcttaaat
     9121 cagaaattga tggatttcta ttgactgtgt ttttttattt tgatacatta gttcacgtta
     9181 ataaatcgta aaattccgat ttgttcattg aaaggagcat ataaatgaaa aaatatcttt
     9241 tgacagctac ggtaatgata ggtgctttac tgtttgcagc atgtggaaat acgaacaagg
     9301 aagcagacaa aaaagaggat ttgacgatcg ttacgacttt ttatccgatg tatgatttta
     9361 caaaagagat cgtaggggat gaaggaaacg tgaaactttt gatccctgct ggaacggagc
     9421 cgcatgattt cgaaccaagt gcaaaagaaa gagcagaaat ttccgatgca gatgttttcg
     9481 tgtataatag ttcagacatg gaattttttg tggattcgtt aaaggattca gtggatagca
     9541 aacagacctt gatgatcgaa gcagctaaag gaatcgaccg tttagaatcg caagaagcag
     9601 acgaacatga agaatcagaa gaagggcatg gtcattcaca tgaatacgat ccacatgttt
     9661 ggctcgatcc ggtattagct ataaaagaag tcagaacgat tgctgaagaa ttaggcgaaa
     9721 aatatccaga taaaaaggaa acttttacga agaatgccga tgcttatatc aaaaagcttg
     9781 aagcactgga tcaaaaatac tccgatgagc taaaagatgc aacaaaccgc acatttgtca
     9841 ctcagcatgc tgcttttgct tatttagcga atcaatacga cttaaaacaa gtagcgattt
     9901 cgggcgtttc accagatcag gaacccacac caagtcgctt agctgagcta aaagaatttg
     9961 tgaagaaaaa caacatcaaa gtgatttatt ttgaagaaaa tgcctcttcg aaggtagctg
    10021 agacattgtc gaatgaaaca ggagtgaagc tagaagtgtt gaatccttta gaaagcttga
    10081 ctaatgaaca aatcaaagca ggcgaaaatt atatttctgt catggaaaaa aatctgaaag
    10141 ctttaaaaga aagtatcaat taagttttag aatggaggtt gtaaggtgcg ttatatcgaa
    10201 atatctgatt tgacctttta ctatgatgat gaacctgtac tagaagatgt ctcttatcat
    10261 gtagatgcgg gtgaatttgt cattttaact ggagaaaatg gagcagccaa gtcaaccttg
    10321 gtgaaagcat cgttaggatt actgaaacct tcaaaaggaa tgataaagat tgctgagaaa
    10381 aatcaagaag gaaaaaaatt aagtatcggt tatattcctc aacaagttgc atctttcaat
    10441 gcaggttttc ctagtacggt gatcgaatta gtccgttcag gaagatatcc aagaggccgc
    10501 tggttcaagc gtctctctga aaaagatcat caacatgtac agcgtgcttt agaagctgta
    10561 gggatgtggg atatgcgaaa tcggcgaata ggtgaattgt ctggcggaca aaagcagcga
    10621 atcagtttag cgcgtatttt tgctactgac cctgacttat ttgttcttga tgaaccaaca
    10681 acaggtatgg atgaagaatc cagaaatgaa ttttatcgtc ttctccgaca tagtgcgcat
    10741 gtccatggga aatcagttct aatgatcacc catgatcatg aagatatcaa acagtatgcg
    10801 gacagacaga tccgactggt gagaaaggag gattctcaat ggcgttgctt tcatatgaat
    10861 tcatgagaag agcgtttctt gccgctatct tcattgccgg aatcgccccg atgctaggtg
    10921 tgtttttagt gatcagaaga caatcgctga tggctgatac gctttctcat gtctctttag
    10981 caggggtagc gttaggattc tttttgaacc ttaatcctac acttacgacc atgctagtcg
    11041 ttgtattagc agctgtgata ttggagtatc tgcgaacgat ctaccgaacc tattcagaaa
    11101 tctccattgc cattttaatg gctggtggtt tggcgctagc gttagtattg atgaatttga
    11161 gcggagggaa ttcagcaaca agtatccaat cctatctgtt tggttcgatc gtgaccatta
    11221 catgggagca agtcatcttt ttaggaatct tattcgttgc catcggtata ttgttctttt
    11281 tattcaaacg gccgatgtat gttctgactt ttgatgaaga taccgcccac gtggatgggc
    11341 tacccgttca tttgatgtcg atgatgttca atgtgattac tggtgtggct attgctgtga
    11401 tgatcccgat tgccggtgca cttttgatat ccgcaatcat ggttcttccc gcagcaatcg
    11461 gtatgcgatt aggcaaaagc ttcaatgtcg tgattgctat cagtgtttgt gtaggatttc
    11521 tagggatgct gtcaggacta actagctcgt tttatctaga tacacctcca ggagcaacaa
    11581 tcacattagt ctttattgct ttgttcctga ttgtaaatat catcaaacga atattggttg
    11641 ccttcagaag aaaaaataac taaattataa agaaaagaag ccttataaaa acgaaaaatt
    11701 ttttataggc ttctttttat tttaataaat accgaactat tttcttattt tttcataaaa
    11761 tgctcgaatt tttgaagaaa aacttatata atgtaaaacg ataaaatttc ttaaaaggag
    11821 ttggaatagg tgaaagtacg tcgcagtgaa cgtctagttg acatgacaca gtatttattg
    11881 aatcacccgc atgaattgat ttccttaaca agctttgccg aacgttatga atctgctaaa
    11941 tcttctatca gtgaagattt gggaatcatc aagaaaacat ttaaagaacg agggtatgga
    12001 accttggaaa caatcccggg tgctgcaggt ggtgtattgt tcatccctga aatttcatat
    12061 gaagaggcga aaaaatatat tgaatctcta gcagaacgtc tatcagaaca agaccgtctg
    12121 cttccaggag gatatgttta tctctctgat ttgttaggag aacctgaact gctgcgtcaa
    12181 gttggaagaa tcattgcttc taaatacctt ggtgaaaaaa tcgatgctgt aatgactgta
    12241 gccacaaaag gcgtacctat tgcccaagct gtgtcctatt atctgaatgc gccattcgtt
    12301 attgtacgcc gggactcaaa aataacagaa ggatcaacag taagtgtcaa ctatgtgtct
    12361 ggatcttctg aaagaattga aaagatggaa ctgtctaaga gaagtttgaa acgtggttca
    12421 cgtgtccttg tagtggatga tttcatgaaa ggcggcggaa cagtcaacgg aatgaagagc
    12481 ttgattgaag aattcgaatc agaacttgtt ggcatcactg tatttgccga atcaaaattc
    12541 aacggtcatc gcgccatcga tgattatacg tcactgcttt atgtcaaaga tgtagatact
    12601 caaacgaaaa atattacggt tgtgccagga aattattttt cagaataaga ggttgtgaaa
    12661 aagccgctta gtttcgagta gtaagaaaaa ttttgtgaaa atggctttcc acattttttc
    12721 ataaatttgg cttaataccg agaaactggc ttttgaacac cgtttatcta gaggttgtga
    12781 aatcagcgtc ctgtcctgag tattagctca ataaacggaa aaattgctcc tcatattttg
    12841 ccgtttattg agctaatacc gaaggtcagc tgattgaaaa acgtttaatg aagaggttgt
    12901 aagcctaccg tttaactcta agtcacaaga aacgatttca aaaaacagct tttcatgttt
    12961 tatgagtagt gtgacttgtg accgaggagt tggcaggaga gcaccggtta ccaaatagat
    13021 aggcccgtta tggtctatct atttttttat ccagaaaacg ctgggataaa agaaaatcac
    13081 ttgttttgtt gaaaaaatgg gtatactggg ataaactgaa atcagcaaac tatatttagg
    13141 agtgacgcag ttggacgcac gttatgcaat tattttggcg gcagggaaag gtacccgcat
    13201 gaagtcaaaa ttatataaag ttttacatcc cgtttctggt aaacctatgg tagaacatat
    13261 cattaatcgg gtcagtgaaa cgaaaccaga tgaagtgatt acgatcgttg gacacggagc
    13321 agaacaagtt aaagcacaac taggagaacg cagtaaatat gcgttacaag cagaacaatt
    13381 gggaacagga catgctgttt tgcaagctgc ttctttcttg gaaggaaaaa aaggaacaac
    13441 gttagttatc agtggcgata caccattatt gactactgaa acgctgaaca atctttttga
    13501 atatcaccaa ggaaaaaatg caagtgcaac gatcttgacg gcacaagcag aaaacccaac
    13561 aggatatgga cgaatcatcc gtgaccatat tgggatcgta gaaaaaatcg tggaacaaaa
    13621 agatgcaaca ccagaagaag ctcttgtaca agaaatcaat acaggaactt actgttttga
    13681 caacgaagct ttgttcgatg cgttaagcaa agttggaaca aacaatgctc aaggtgaata
    13741 ctatctaaca gatatcatcg aaatcttaaa agaagaaggc catacagttg ctgcttatca
    13801 aacggatgat tttgaagaat caatgggagt caatgatcgg atcgcgttag ctaaagcaaa
    13861 tgagatcatg agaaagcgaa ttaaccagat gcatatggtc aatggcgtaa gctttgtcga
    13921 ttcagcaacg acatacatcg atgcaggcgt agaaatcggt ccagacacat tgatcgaagc
    13981 aggtgtacaa atccaaggaa atacagtcat tggttctgat tgtgtcatcg ggtcacattc
    14041 aaaaatcgta gacagtagga ttgaggatca cgtcgtcatt gaaaactcag tcattgaaag
    14101 cagccatgtc aagaaacatg cagatgtggg accttatgca catttacgac caaaagctga
    14161 aattggcgaa aacgtccata tcggaaactt tgtggaagtg aaaaatgctc aaatcggcaa
    14221 aggaactaaa gtcggtcact tgacttatgt tggtgatgct acgctgggag aagaaatcaa
    14281 tgtcggatgt ggtgttgtct tcgtcaacta tgatggaaag aacaaacatc atacgactgt
    14341 cggcgaccac agctttattg gttctagcac gaatatcatc ggacctgtcg aggtagctaa
    14401 aaacagttcg attgctgccg ggtcaacaat tacagacaat attccagaat atgcgctggc
    14461 aattgcgaga gcaagacaag tcaataaaga aggatatgca aaaaaactcc cttatctaaa
    14521 ctaagacttt tgcaaaatca cttgatttat ttaaaaaata tgactactat ttattaggta
    14581 gaattctaga gttgtgaaaa agaaagtgga ggtccccatg tcaaaacatt actttgaccc
    14641 aagattaaaa atttttgcgt taaattccaa tcgcccatta gcagagaaaa ttgcagcagc
    14701 agtcggcgtc gagttaggaa aatcatctgt tactcaattt agtgatggag aaattcaagt
    14761 aaacattgaa gaaagtattc gtggatcgca tgtctatgtt attcaatcaa cgagcagccc
    14821 agtcaatgat aacttgatgg agttgctgat catgattgac gcactgaaac gagcaagtgc
    14881 aaaaacaatc aacgtagtca tgccttatta tggctatgcg cgtcaagacc gtaaagcacg
    14941 tgcacgggaa ccaatcacag cgaaattagt agcaaacatg attcaaaaag ctggtgcaac
    15001 aagaatgctg actttggacc ttcatgcagt ccaaatccaa ggtttctttg atattccagt
    15061 cgatcatttg atgggtgcgc cacttattgc caattatttc ttagaaaaag gaatcaaagg
    15121 cgatgatgtt gtcgttgttt caccagacca tggtggtgtc acacgggcac gtaaattagc
    15181 ggaatttttg aaatctccga ttgcgatcat agacaaacgc cgtccaaaag caaatgttgc
    15241 agaagtaatg aatatcattg gacaagtcaa aggcaaaact tgtgtactga tcgatgatat
    15301 gatcgataca gcaggtacga tcacattggc tgccaatgct ttgaaagaag ctggtgcaac
    15361 tagtgtgtat gcttcatgta cacatccagt attatccggc cctgctttac aaagaataga
    15421 agattcagct atcgagcgtc tagttgttac agactcgatc tatcttccag aagatcgtaa
    15481 aatcgacaaa atcgatgaag taagtgttgg cggactaatg ggagatgcaa tcaaacgtat
    15541 ccatgaaaac aaaccagtta gtccattgtt tgaaacaaaa aataaataat aaataaactc
    15601 acaaagggga gtccaattgg gctgagattg aatctatttc taaacccttc gtacctgtat
    15661 cggttatgcg agcgtaggaa ttgtgaataa acagctgttt gactattttc gaaagctgct
    15721 tcctcctttg tgactttttt attcaaagga ggaagttttt ttatgggaaa aaatagagtt
    15781 tgggtagaag gaacgattgt tgcagcgtta gcgatggtgt tgtcgttgat tccgatccag
    15841 atcggcagca gtttttctat ttcattaggg cagattccat tgactctttt tgcgttaaga
    15901 agagggacaa aggcagggat gctggcaggt ttcatttggg gcatcctaca ttttccgttg
    15961 ggacaagttt attatttaag tgtcgttcaa gtgttgattg agtacccgat tgcctatact
    16021 tttgcaggcg cagcagggct catggcttca aatgtccaaa aaggctatgt agacgataat
    16081 acgaaacaaa ttagaaaaag catcgtgttt ggtgcattga ttggcgcact ttccagatat
    16141 ttttggcatt ttattgcagg agtggttttc tggggagcgt atgcactatg ggggatgaat
    16201 ccttggctct tttctttcgt aatgaatggt gccagcgggt tgagtacagc tattgtaacg
    16261 atcatcgttt tattagggat ccaacaaacg actccttcga tttttgtgcc agataaacga
    16321 caggagctgt aaataaaaaa gaagcaagaa acttgagaca gaatcatgtc ctagtttctt
    16381 gtttcctttt tattttttga taagaaatag atctacttca atggcagata agaaagatcg
    16441 gcatcttcta ccaattgcca gtgtcgatcg gtgacagagt agattaaagt agcgatgatt
    16501 tcctcttctc gataccgggt gccttttaat ttccaaatat aatcttcaat cacatagatt
    16561 gggatacgat atttaccatc gatcaaacca acttgatcaa aagaaaaaat gatcccttcc
    16621 tgcatcaaat tacgcatttc tttcaataat tccacactga ttttttcaac aggtgtttct
    16681 tttttgaaaa aagatttttt tacattgatc ttatctgaca agatcgaact taatttacta
    16741 gcaaaaatcc tataaaattc atctaaatag cgataataaa tccgattcag ctgatcatta
    16801 agatcgaagt atacaaaatt attctgtagt ttataaaaaa acggcgaatg aagatgtgtt
    16861 ttcatatgac caaaatataa taattctgaa atttctaaag gtgtcaattc tttcagcatc
    16921 gttaaatcag taaagtcgat ccacttcaat tcatcgaatc cacgcctgtt acgagaagag
    16981 gaaaagaaac gctgaatatt ttctgtacca cggattactt tcagccctgt atgggattca
    17041 tattctccag cagaagcaga tggatcaagc aagagcaagt tttgaggaaa atgcacgata
    17101 ctgtttgcaa agtcagtagc agtcagccct tttgttaaaa cagcattact tgtggtatca
    17161 atatgtacat aaactgactc agtcatcatg ttcctcctct tatttcctct taatctcttt
    17221 tattttacaa catttgcatt aaaaaagctt gtttctttta ttttaaaatt ccattacaaa
    17281 atcacgtttt cattgaaaac actctcattt tcaattaatt tttgtgattt ttgtattttt
    17341 ttatttaaag gaggttttat gaattgtttt cttatggaat ctacaaagtc gaatacgaga
    17401 ctcatgacct ttttatgacg tactggtata cttgaagaaa aaggagagcg cgatggagat
    17461 aaaaaaagtt tacttttcac agaaaatgtc gctgtattcg cctatccagc aagcaaaaca
    17521 accccttcca ttttttcaaa gtcatcaaga aagtagagac gctttttggc gttctgcaaa
    17581 aaagcaatgt gcttttaaag cagtatcgaa aaaatatacc gtctatttta ctattgctaa
    17641 aagtagagga gatgttattt ttaataatct gttgattgaa ggtgaaactt ttgaatggaa
    17701 aaaatttttt cggtatatgg aagcttgcgc tcgttttttt attaaagaaa actgttgttt
    17761 tttatttcaa tctcctctat cagaagaatg gttgcgtatt tttcataaaa acggctatca
    17821 aggaaccaca caattaaata aaaagctggt ctatcataca gcacttgttt taggtggagg
    17881 aggagcgcac ggtgcttatc agattggtgt gtggcaggcg ctgaaggaac atgatatcaa
    17941 ctttgagatt atcacaggta cctcggtagg tgcgttgaac ggcgcattga ttttacaagg
    18001 agatatgaaa aaagctgtga atttatggaa aaagctgtcg acaagacaag tgcttgcgtt
    18061 accagaaatg gctgcagttg aagaccttcg tgaacgcttc tatcgagaaa gacgccaaat
    18121 gacgaaaaca gcactaattg aaggcggggt ttcttctgct ccattggaaa agatggtcaa
    18181 agacaatttg gatttgtcct tactcaaaca taatcccaaa atacgtttat atacggtgtc
    18241 tactaaactg ccagaactag cggaagtagt gactgatatc caacagacaa aaacagaaga
    18301 aataccagat tggatactcg cctctgcttc attttatccc gctatggctt atcgaaagat
    18361 cggaaaaaat aaatatgcag acggtgggta tcgcaacaaa atacctattg atattgcgat
    18421 taacaaagga gcaacagagg catttgtcgt agatgtgcaa gggccaggcc cagcgaaaaa
    18481 aattcgtatg ccagacattt ttatccattg gaaatgtcaa acattgtgga cactaggcag
    18541 ctttctgctt tttgatagcc aaaggaacca attaaattta caattaggct atctggaaat
    18601 gaaaaaacga ttaggctatt actatgggaa ctggtataca tttcattctg ttaaacctgc
    18661 aagaacatac tggcgaggat ttttgtctta cttaacaaaa gaaattcagt tagaattatc
    18721 ttttttcaaa agcataaaat tttggcaaaa actgcgtaat ttatataaga acagggtcgt
    18781 accagagaca tgcggattgg cgatgctaga acttttagca aaaaaacatt ttttactgcc
    18841 caatgaaatc tatcaagtag acatgatgat tcaaatgatc tgccaacaag atataaaaag
    18901 tatgccggat gatttattgg cacaaattgg tcaattaagt actgaagaat ggcgaaggta
    18961 tcgaaaatac cagcaaaaaa tacaaaatga gcgcacgaaa actgtttatt ttgattatct
    19021 gctacaagga aaacgcaagg atcgtttgca gcatgaattg cttaagcaac cagttgacac
    19081 attactgatc ctatatttat attatctcaa ggaggaacaa ccatggcaca agaattttca
    19141 tatgaaatcg tagaagaaat cgctatattg tcagaaaaca acaaggggtg gcgtaaagaa
    19201 ctgaacctag tcagctggaa tggtcgcccg ccaaaattcg atcttcgaga ttgggcgcca
    19261 gaccatgaaa aaatggggaa aggcctgaca ttgacgaacg aagagtttga acaactgcaa
    19321 aaagcaatag aaaatatgta atgaggaagg accctgtcga atgaaaagta tgactggttt
    19381 tggcaaagcc atccgcgaaa caaaagcgta tcagttagat attgaaataa aaagtgtcaa
    19441 ccagcgtttt ctagatgttc aaatacgaag tcctaaaatc ctgaactttg ttgaaaacga
    19501 ttttcgtcag cttattaaac agcagttaag ccgcggtcga atcgaagtat ttgtgaatct
    19561 agcttatatt ggcaaaaacg aaaaacagat cgtcatcaac tgggaattga ttgattcact
    19621 tatcactcaa ttgacagacg gaattcaaca acgatacggt agtcaaaatc aagtgtcaat
    19681 tagtcggatg gcggaacgct tcgtgctgga cgaaaacttc gtaacggtag aagaaaaacc
    19741 ggaagagagc atggaagaat tggcacagtt agctctagat acattgaaaa gcgcacttga
    19801 ttctattgag gcgagtcgag aaaaagaagg tcaagcttta gctttagtgt tgaaaaagaa
    19861 tactgaagaa ttcaaagaag tactaaaaaa actcgatagt tttgtagaaa tctatgaaaa
    19921 ggaatttcaa ataaggtatc aaaaaaagct ggaggaattt cttggagcga aagtcgatga
    19981 gcaacggttg ttgacggagc tagccatctt gttggaacgc ggagatattc acgaggaact
    20041 ggatcgtctg gcgatccatg tacaaaaact agatgaactt atcgaagcac aatgtcctgt
    20101 aggtcgggaa ttagatttcc tgatccaaga aatgaatcgg gaaatcaaca cgatcggttc
    20161 caaatcaaat gcaatcgaaa ttaaaaatca agtcgtccag tctaagacga tcttagaaaa
    20221 aatcagagaa caagtacaaa atattgaatg acgaaagagg tagagataat gaaatattat
    20281 gacgtaacgt tccacgaatt aagtggaaaa actgtggtta aacgggatat cccttctgaa
    20341 aaaaacggct ttgacgtatg gaaagatgcc tgtgcagact ataatgagaa cgaactgttt
    20401 attttgatca acgacggtgc ttacgtgacg atgaatcgga aatttattgt tcgaattgac
    20461 acagaagagg tagaagatcc gacagaaaaa gcacgttctc gaaaagatga gatcatgggt
    20521 gtagtgaata ctttgtccaa tatgggcttt tagcaaggat gctgaacaaa gaaatacaat
    20581 aaataaacag aattggattt cttcgcttag cttctttgct ctaggaatac atttaaaggt
    20641 tgtgacaaaa ttcgtgtcac aatctttttt tattttgaat aagtgatgtt caaaaaccat
    20701 tttctcggta ttaagccaaa tttatgcaaa aatgtgagaa actattttca caaaattttt
    20761 cttcatactc gaaactaatc ggcttcttca cgacctctta tacacggcgt tcaaaagctt
    20821 gcatctgcga taataacccc aaccaaacga aaaacatgag aagctgttta tgtttggctg
    20881 ttcttattac ttgatgcagg acagcttttt tacgacctct tggtacaggg tagctttttt
    20941 accaatttct agctataatt aaagataacc ttgaaagttg cagaaaaaaa gtgcaaaata
    21001 aacgataagt acaaaaagac tagttgaaag gacgtcgtca tgtcagagcg tggattatta
    21061 atcgtgttgt ctggtccatc cggtgtagga aaaggcacag tacgaaaagc aatttttgag
    21121 agtgaggata atgattttca gtattcgatc tcaatgacta cccgtaaaat gcgtgaaggg
    21181 gaggtcgaag gcgttgatta tttcttccga agcaaagaag agtttgaatc aatgatcgaa
    21241 gctggagaga tgctggaata cgcagaatat gtcgggaatt attatggaac tcctttgtca
    21301 tatgtcaaca agacattgga tgaaggaaaa gatgttttct tggagattga agtccaagga
    21361 gcaaaacaag tcaaagaaaa agtgcccgat ggtgtattca tctttttgac tccgccagat
    21421 ttagcggaat taaaatcacg gatcgtaggc cgaggaacag atgcagatga agtaatcgaa
    21481 aaacgaatga aagttgcaaa agaagaaatc gaaatgatgg ctctttacga ctatgctgtt
    21541 gtaaatgatg aagtgccgct agcagttgaa cgaatcaaaa acattattgc cagtgaacat
    21601 tttcgagtag atcgcgtaat cggtaaatat attaaaatgt taaaggagat gtagtcttat
    21661 gatgttaaaa ccttcaattg actcactatt agatcgtgta aattcaaaat attctctagt
    21721 gattttagca agtaaacgcg cgcatgaatt ggatgcaagt gcacagccta ctctagattc
    21781 ttttgattca gtaaaaagcg tcggtcaagc tttagaagaa atcgatgcag gaaatgtagt
    21841 caatgatcca catcctgaat taaaacgtga acgtttgaag atggaagaag aggaacgtca
    21901 agcacaaaaa gagcgtgaac aacacgaatt ggaatcacgt atccgtgaag agcaaaaaat
    21961 gtaataaaga aaaatagagc gaacatctaa aaagaaaagc aaacccagct gtttcctttt
    22021 caaacattcg ccacttattg tctggtagaa aaggctgtgt ctgaactttt tgacacagtt
    22081 cttttttttc ttatctgctg ttaatttttt gttaggattt ggcagaagat aagacaaatc
    22141 catcgatctc tagtaaaata ggcaaggaac tttttgtcgc ttgataagaa aagacaaaaa
    22201 aaggaggaaa cgaaccatga cattaacagc tgaagtcatt gtggacgtac ctactatgca
    22261 gaccgatcag ccattcactt atttggttcc ctctgaaatg gaaacagctt tgcaagtcgg
    22321 gatgcgagta gaagtccctt ttggtaatgg caatcgtcat gtccaaggat ttgttatggc
    22381 tatccaaaaa tcagaagaat cagctaatcc atctttgaag gcagttattc gtttgctaga
    22441 ccttgctccg gtggtcaatg aagaattact ttcattagct gattatatga aaaaaattac
    22501 ttacgcattc aaaatcactt gtctacaaac gatgcttccg agtgtgatga aagcagaata
    22561 tgataaattg atctatcctt tggcagacac accagaagtt caggtattgt ttgatcagcg
    22621 agaagtgatt tcctggaagg aagcagaaga agaaggcagc ctttctcaat tgattcgctg
    22681 gcgccaagag caattagtag atatcaaata tgaagtccat acacgaaata aagtaaaaac
    22741 gattcgtttg gtccgttctt tgttaactga aaagcaaatc gaagaagaat gggcaaaatt
    22801 acggcaaaat gccaagaaac aaaaagaact tcttttgtgt ttatctgaac tgagccaaga
    22861 agaaccaatt gcttatttca aaaataaagg aatcagtact gctgtcttga atcaaggaaa
    22921 agaaaagggc tggctggagt ttatagaatc agaacgttat cgcgaccctt ataaagaccg
    22981 agtatttgac caaacgacgg cattggaact gaatgcagaa caaaaaaatg ctgtcgaaca
    23041 aatcatcaca gcaggtcagc agcaaaaaga tcaggtcttt ttgctggagg gaatcactgg
    23101 cagcgggaaa acagaagtat atcttcaggc aatcgctgat gttttgtcag aaaataaaac
    23161 ggctattatg ttagtgcctg aaatcgcatt gactccgcaa atggtcgagc gattcaaagg
    23221 acgcttcgga gaatcagttg cggtcttaca tagcggactt tcccaaggtg aaaaatatga
    23281 tgaatggcgc aagattgagc gggaagaagc gcaagtagta gtaggcgcac gatctgctat
    23341 ttttgccccg ctgaaaaata tcgggctgat cattatcgat gaagaacatg aatcaagtta
    23401 caaacaagat gagacaccgc gttatcatgc gagagatcta gcaatttggc gcagtaaata
    23461 tcatcattgc ccaatagttc taggaagcgc gacgccctct ttagaatcaa gagcacgagc
    23521 tcaaaaaggc gtttatcagt tattgcagtt aaaccaccga gccaaagcag ctgcacagct
    23581 tccagctatt gagatcgtag atatgcgtga agagtttcag aatcatcgga cctctacttt
    23641 ttctgctaat ttgcaggaaa aaatacaaaa tcgattagac aaaaaagaac aaactgtcct
    23701 actgctcaat cgtcgaggat attcgtcttt tgtgatgtgt agggactgtg gatttgttct
    23761 tccatgtcct aattgcgata tctctcttac actgcatatg gatacacgtt caatgcgctg
    23821 ccattattgc ggacatgaag aacctattcc caatcgctgt cctaattgtg gcggcaataa
    23881 aatccgctac tatggaacag gtactcaaaa agtagaagag gaactccgag aactttatcc
    23941 gcaagctagg attttgcgta tggatgtaga taccactcga agaaaagggg cacacgaaca
    24001 gattttacaa aaattcggtg caaaagaagc agatatatta ttagggacac aaatgattgc
    24061 taaaggtttg gactttccag aagtgacatt agttggggtg ttgaatgctg atacctccct
    24121 gaatttaccg gattttcgtt ctagtgagca tacattccaa ttgcttaccc aagtttcagg
    24181 acgagcaggg cgagcggaaa aagcaggcga agtcgtgatc caaacattca atcctcaaca
    24241 ttacgccata gaattggcta aaaaacaaaa ttatgagcag ttttatcagc aggaaatgca
    24301 tgtacggcat cgtggaggct atcctcctta ttactttact gtgaaaataa ccgccagcca
    24361 tcctgaagaa caagtagcgg caaagaaaat ctttcagatt gccaatcagc taaaagaagt
    24421 gttaagccct caaagcttgc tattagggcc aacgcccagc atgattttga gagtgaaaaa
    24481 ccgatattat tatcaactaa tcatcaaata taaacatgaa ccgaatcttc ctcatgtttt
    24541 ggatgaaatt ttgaatggaa gccagaaaga acagcgacaa ggtctttttg tggcaattga
    24601 caacgaacca ttaaacttta tttaagaaag ggccaaatta tgcgctatcc tatattgatc
    24661 caccctaatg ataaattaaa aagaactgct cagccaatcg atgtaatcac ggacgagaca
    24721 atcgctttat tagacaattt atatgagaca atgattgcga atgacggaat agggatcgct
    24781 gctcctcaag taggacaaaa caagagaatt gctgtgatcg aagtagatga aggcgagaaa
    24841 tttgaactga ttaacccaga aatcatcgaa gcaaaaggtg aaagccttga cgtagaaggt
    24901 tgtctcagta ttccgcatgt ctatggaacg gtcaagcgag cagatgaagt gaccgtgcgt
    24961 tattatgatc gagacggaga agagatagaa gtgacggctt ttggctattt agcccgtgcc
    25021 ttccagcatg aaatcgatca cttagacggt atcttgttca ttgagaaaat gatacagcag
    25081 atcccggaag aagaacttga agaatatatg gaggaacatc ttgatgacta agctagtatt
    25141 tatgggcaca cctgcttttt ctgtgccgat tcttgaaggg ctgatagaat caggttacga
    25201 gatccaggca gtggtaacac agcccgaccg tccagttggg cggaaacgag taattacgcc
    25261 gacaccggta aaaaaagcgg ctctgaagca cggtatccgt gtgctgcagc cagagaaaat
    25321 ctccggttcg ccagaaatgg aagaaatcat tgaattggtg cctgatgtga tcgtcactgc
    25381 ggcatttggc caattcttgc ctgaaaagtt attgcaagta ccaaaattag gggcaatcaa
    25441 tgtccatgct tctttacttc ctaaatatcg gggaggtgcg cctgttcatt atgccatcat
    25501 gaatggggag aaagaaactg gtgtaacgat catggaaatg atcaaaaaaa tggatgcagg
    25561 cggaatctat gcgcaagaaa gcataccaat cactaaacaa gatgatgtag ggacgatgtt
    25621 tgaaaagctt agccttttag gaagaaagct attgctagaa acattgccca atattttgga
    25681 tggacaaaaa ccagtacctc aagacgagtc agaagcaact ttttcgccaa acatcacacg
    25741 ggaacaagaa gcaatcgatt ggcataaaac tgcagaagaa attgacaacc aagtacgcgg
    25801 aatgcgtcct tggccaattg cattcacgac ctatgagggc atacgctgga aattattatc
    25861 ggttgaagta ttggcagaaa ctacagacaa agcacctggt acgatcatcc ggaaagacaa
    25921 aaaaaatcta tggattgctt gcggtaaaca gaccgtctta gccatcaatg aattacagcc
    25981 agctggtaaa ggaaaacagg ccgtacatga tttccttaac ggaagcggtc agcaagttca
    26041 gattggtcag caggtgaaat aatgaaaaaa atccctaaaa aaatgaaaaa atcagtacga
    26101 tatatggcat tactgacatt agagcgagtg aacaagggtg gcgcctactc gaatcttttg
    26161 ttgaatgaga tgatcaaaca aagcgagcta aatcaaaaag atgtgggttt atttacagaa
    26221 ttgatctatg gtacgattag ccatcagcga ctattggaat tttacgttgc accattgatc
    26281 gctaaagcaa aaaaagtgga tgactgggtc aagatgcttc tttatctttc tgtataccaa
    26341 ttagaatttt tagataaagt ccctgcacat gctattttaa acgaggcggt cgaaatcgca
    26401 aaagtaaaag gaaatcctgg gacagggaaa tttgtaaatg gtgtcttgag gaattatcag
    26461 cgtcaaggag caccagatct gcaaggcatt gctgatccta tcgaaagact ttctgtcaaa
    26521 atcagcctgc ctctatggat gacgaaacga ctggtggatc aaatcggata cgaagaaact
    26581 gaaaagctcg gtttatcgct atatcagcct agccatgcta gtgcccgagt agatacacgc
    26641 cgactttcca gagatcaggc catcactgtt ttgcaagaag aagagatcac agctgaaaaa
    26701 agccaagtca gtccatacgg tatagtagcg aaaaaagggc atttagcttc tagctcattg
    26761 ttccacgatg gcgtcatgac gatccaagat gaaagttcga tgcttgtagc gccgtctatg
    26821 caaatcgagc ctgaacatca tgtcttagat gcctgtgcag cacccggagg taagacgacg
    26881 catattgctt cttttttaga tccgtcatgc ggtggagaag tggtcgcttt agatgtacac
    26941 gaacacaaag tcaaactcat tgaagataat gcagcacgtc taggtgtctc ttctgctgtc
    27001 tacgctcaaa agatggatgc tagagaagtc agtaaacagt tcgaagcaga gcagtttgat
    27061 cggattttag tagacgcgcc ttgttcaggt cttgggctgc ttcggcgaaa accagatatt
    27121 cgttataaaa aacagccgga tgaattagcg aatttaccaa aaatccaatt ggaaatttta
    27181 gaaagctgcg caccaacttt aaagtcttcc ggtattctga cttatagcac ttgtacgatc
    27241 ttaaaagaag aaaaccaaga cgttgtttct gctttcttgg aaagacaccc tgattttgag
    27301 aggattgatg ttttgaccaa tcctatgcta caatcatcaa tcgaagagaa aatgttgact
    27361 ttgtatccgc atcagtttta cacagatgga tttttcattt gttgtttgcg caaaaagtaa
    27421 tgaggtgaaa aaatgcagat tgaatatcaa tcagatgtag gaagacgacg taacacaaat
    27481 caagactatg ccagtgtatt taccagccgt tcaggtctca agcttgctgt attagcagac
    27541 gggatgggtg gtcatcgtgc cggagacatt gccagtcaga tggcggtaac gaatctaggc
    27601 gaagcttggg aaaaacaaga agctgttgac gatgaaaaaa tcgcccaatg gttcattcaa
    27661 tcgattcaag aagaaaatat gctgatttat cagcgtggac aagaacagcc ggaatataac
    27721 ggtatgggaa caacaatcgt agcggccgct ctgtcagatg actggttcac gattgccaat
    27781 gttggggata gcagagctta tttgattcgt gaagataaaa tcatgcaatt gacggaggat
    27841 cattctttag tcaatgaact agtcaaatca ggagaaatca gcgaagaaat ggcagtgaac
    27901 catccaagga aaaatatttt aacacgttct gtcggcatgc ctggaacagt cgaagtagat
    27961 gtttctactc atctttggca actaaaagat cgactgctgt tatgttcaga tggcttgacg
    28021 aacatgctgt ctgaagaaga aataggaacg atcatcaatc aagaaggccc attgtctcat
    28081 aaagtaactg agctgattga tcgtgccaat gaagcaggcg gagcggataa tatcaccgtt
    28141 cttcttatcg aatataagga ggacagtgta tgatcgagtt gggcaaaaaa ttaaatggcc
    28201 gttatcaaat cactggaaat atcggtagcg gtggtatggc aaatgtattt ttggcgcacg
    28261 atttaatctt ggatcgtgat gtagcagtta aagtcttacg atttgacttt caaaatgacc
    28321 agactgcgat tcgacgattt cagagagaag ctttagcagc aacagaactc gttcatccaa
    28381 acattgttag tgtttacgat gttggtgaag aagacaacat gcagtatctg gtgatggaat
    28441 acgtcaaagg aatggacttg aaacgatata tccaaacaca ttatcctgtt ccttatgaaa
    28501 cagcagtaaa tattatgcag cagatccttt ctgctatttc tttagctcat aaccatcaaa
    28561 tcatccaccg agatttgaaa ccccaaaacg tactgatcga taatgaaggt gttgtaaaaa
    28621 tcacggattt cgggattgct atcgctttat ctgaaacatc gatcacgcag acgaatacga
    28681 tgctaggatc tgttcattat ctgtcccctg agcaggccag aggaagtatg gcaacgaagc
    28741 agtcagatat ttatgcttta gggatcatcc tttatgagat gttgacgggt agcgtaccgt
    28801 ttgatggtga atctgctgta acaattgctt taaaacattt tcaagatgat cttccatcta
    28861 taaaagcact tgatccaaat gtgccacaag cactggaaaa cgtgatttta cgggcaacgg
    28921 ctaaagaacc tgcagaccgt tataagtcag ccgaggaaat gtctgatgat ctttctacag
    28981 tattatcacc agctcgtgca aacgaagaaa aatggcagcc tcatgtgatg gataatgaaa
    29041 caaaagttat cacacccctt acggaagatg caccaatgcc tgattcattc aaatctatgc
    29101 ctctgccaaa agacaagcca gaaaacgaaa ccttaccaga ggaggaagaa gttctgcctg
    29161 atcctaaaaa gaaaggacag aaaaaatggt ggctgatcct attagctatt cttgctgttt
    29221 taggaatcgg aacagctgta ttctttgctt ctggcgggcg aggagaagtt gttgttcctg
    29281 atgtttctga tctttctgaa gcttctgcta gagaaacact cgcaaaagcg aaattagaag
    29341 tagcaacaaa aacagaagaa attgcagatg acaaaatcga agaaggtaat gtagtgaaga
    29401 cagacccggc agcgggaacg acagtcaagc aaaaacgcga agtcactctg tatattagta
    29461 gcggcacgaa aaaaatcaaa ttagatgatt acagtggtaa aagttatgaa gatgccgaag
    29521 caagtttgaa agaattaggg ttttctgctg acttgattca aaaagaagag gaattcagtt
    29581 cagaggtaga aagcggaatg atcatttcgc aatctccatc agaagataca gaagtcgatc
    29641 ctaaaacaga cacgattact ttcacagtca gcaaaggacc agaaacagtg actttgagta
    29701 gtcatgtagg agaaaactac gaaactgttc gtaattggct tgttacacaa ggattcaata
    29761 tcaataatat tagtgctact tatgactata gcgacactgt accatcaggt tcgatcatca
    29821 gtcaggatcc agcctcgggt gaagttgtag cagaagaaac ttacatcaat tttgtcgtaa
    29881 gccaaggaac agagcctaaa ttgaatgata tctccggcta tacaaaaagc gaagcacaaa
    29941 gttatttagc tagtgtcggt gcagaatata tcgggcatga gacttccgaa tattcaaaca
    30001 cggtggataa agataaagtg attcgtacag taccaggggc aggaacgaca attaccaaag
    30061 gaatggttgt gaacgtaatt tactctaaag gaccagatcc agcgtcttct agttctgaaa
    30121 aagactcttc agacagttct agcagctcaa gtagctccag tagcacaagc aactcttcaa
    30181 aagaagaatc ttccagctcc acaagttcat cagctagcac ctcagaaagt tcaaaataag
    30241 tgctgaataa aatatatcag aagggaatgc gacattgatt tgttgcattc tcttttttgt
    30301 gtttctatct cttttgatca attttcaagc catatacttt gagaagtttg catggaataa
    30361 cacctacttt cctcttcgta tggtaaaata aacaaaaacg gaggtggcac ttatcaaagg
    30421 acaaatcaga aaagcaatca gcggttttta ctatgtatac gcaaacggag agacctatca
    30481 aacaagagga agaggaaatt tccggaatcg gaaaatcacg ccgcttgtcg gagatcaggt
    30541 gatctttgag agtgaaaatc aaacagacgg ctatcttctg gaaatcttgc cgagaaaaaa
    30601 tcaattagtt cgcccaccag tcacaaatgt tgatcttgga atcattgtca caagtatcgt
    30661 agagcctgca ttttcttata atctacttga ccgctttcta gttaccttgg aatacgagtc
    30721 gatcgaaccg attatttttt tgacaaagac cgacttgtca gacgacccag aaaaaatcga
    30781 agagatcaag caaacctata ctaaaatagg ctatccggtg attgtaccca atcatacggg
    30841 tgatacgaca gaactgatcc gttacttccc agaacggcta tcggtcttta tgggtcagtc
    30901 tggagcagga aaatcgacgc tactgaatca aatatcaccg gaactgaatt tagcaacagg
    30961 agagatttct gatgcacttg gaagaggacg gcatacaaca cgtcatgtgg aactgattcc
    31021 tatctatggc gggttagtag cagatacacc aggatttagc tcgattgact ttttgacaat
    31081 tgaagcagga gaactgccga gacaatttcc tgaatttgta gaagcttctg tccattgcaa
    31141 atttagagag tgcatgcatg ctaaagaacc tggctgcgaa gtaaaacata gagtagaaac
    31201 aggtggtatc gcacagacac gttacgacaa ttatttgcaa ttcttacaag aaatcgaaaa
    31261 aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa taggaggaaa caagaatgaa
    31321 gatagcacca tccattttga gtgcagattt tgccaattta caaagagaca ttgagttagt
    31381 ggaaaaagga ggcgcagatt acatccacgt agatgtaatg gatggccgat ttgtcccaaa
    31441 tatcacatta gggccaaata ttgttcaggc aatccgtcct gttactaaat taccgttaga
    31501 cgtccatctg atgatcgttg atccagaaaa ctacattcca gcatttgcaa aagctggagc
    31561 agatatcatt actgtccacg tcgaagcaac tcctcatatt catcgggcac ttcaaatgac
    31621 aaaaagctta ggagttaaag ctggagtagt tatcaatcca ggaacaccag tctccatgat
    31681 caaacatgtt ttgcctttgg ctgatcaagt actagtcatg actgtcaatc ctgggttcgg
    31741 cggacaaagc tttattgaag aaacagtaga aaaaattacg gaactcgctg atctacgtag
    31801 agagaacaat tggacttatg caatcgaagt agatggcgga atcgttccag aaacagcaaa
    31861 aatctgtaaa gatgcagggg cggatgtctt cgttgcagga tcatatatct atggagccga
    31921 aaatccagtc ggacaaatcg aacgattaaa agaggcatta gactaaatga aaattttatt
    31981 agctgctgga ggtaatccgc aaaactggcc gcagtctctg ctgtccaaat acgatgccta
    32041 tgtcgggata gaccgtggtg cgctgtacct gcttcaagca ggctacccag tagatctggc
    32101 agtgggtgat tttgattcat tgtcgaacga agagcagaag gaagtatttg caaaagccaa
    32161 agagtatacc cgttcccctg cagaaaaaga cgatacagat acacagttag gtatcttaaa
    32221 aacatttgaa cgttacccgc aagcggaagt cactttgatt ggagctacgg gcggaagatt
    32281 ggatcatctt ttagccaatc tctggttagg tatggaacct cgcttcaaac cctttgccca
    32341 tcagcttatt atcagagatt gtcaaaatta tttgtcttat tatttgcctg gagagcatat
    32401 agtaagacag ataccagata tgaagtattt agcttattgc tgcttgactc ccgtctcgaa
    32461 tcttacatta aaaggcagca agtatgtatt ggaaaataaa gaagtagaat atccgacatc
    32521 ttatgcaagt aacgaatttc tttcagatac cgcagaattt tctttttcag atggtatgat
    32581 tgcagttatt caaagcaaag attgatccat aaacaaataa aaaaatttgt ctttcaggct
    32641 aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa gaggagcaac ttaccttttg
    32701 tttcacggca tttagtgata aaattgaaca caagagtcta attaattcga ggtgtctttg
    32761 tgaagaagtt caatcctaat aaaaatatta ttattacctt ggttattgtt atcatcgtgg
    32821 tgacgatact aagtgtgacc gctgcgcagc gagcgaatga aggaaagccc aatttcttcc
    32881 aatccatcgt taatgacagt gttgcaatgg ttgataaaac aatctctgcc ccagtacggt
    32941 gggtacgaaa tggagtagat tctgtccata atcttttttc aacttatgcc gaaaatgagc
    33001 ggctaaaaga aaaaatcgat agctatgatg acgtttcaca gcaaaacaag aattacgaga
    33061 gagaaatcga agcgttgaaa aaagaacttg agttgaatca gacgctgact agttacgaaa
    33121 aagtaacggc aaatgtcatt actcgttcgc ctgacagttg gcaggacatg ctgatcgtag
    33181 acaagggttc taaagacggt atcgaagcaa atatggcagt catgtcgcaa aaaggattga
    33241 ttggccgagt gatcgaggtc aatacggctt cgtctaaaat cgaattactg tcatcctcta
    33301 atgaaagctc caatcatttt ccagtacggg tatcttcggc taatggcgaa gcgtttggtt
    33361 tgcttaaaaa ctatgatgaa aagctccatg ccttagtggt gacccaatta actggtgata
    33421 cggatatcaa agaaggggat gttgtccaga catccggtct tggagggaat tctccagcta
    33481 acttgccgat cggtacggtt attaaaacga aaccagatag ttatgggctg gatcgggaag
    33541 tttatgtgaa accttatgca gaaatgtatg acgtgtcagt tgtgacgatt gtccaacggc
    33601 tagcggagga ttagtccatg ttgaagaaag aaacaatgaa gtactatctg ccaatcgttt
    33661 tgttcttttt gatgttgata gatggtcatt taacaagaat gctaggggag tggtcgaaag
    33721 gcacctatat gtcaaatgcc cactttctga tattggcatt attatgttgc agtatggcgt
    33781 ttgaaaaacg ttatttactg attaccacga ttgttctcgg ggctatctat gacgcttact
    33841 atattggcgt tatcggtatc tatgcagtag ctctcccttt aattgtatgg ttgatgtatg
    33901 taatgaaaga cgttatccat gtcaacatct ttactgaatt tttcagtatg atcatctttg
    33961 tcacgggtta tgaattgttt acgatggtgg tccagttgat ttttaaatta gcagtagtaa
    34021 ataacacgta ttttattaca aggtttttag gacctacact gctgttgaac atgattatat
    34081 ttgtattatt catttttccc tttaagaaat tattcagtga tgaataattt ctttttttat
    34141 ttttaggtcg aaagcgaaaa agaatcgttg ttatcttaag aaatgctaga cttaacgcgg
    34201 ctttttcatg atcattttct gaaacgaaca gttttgtcat ttttaaaagt tgttttgaaa
    34261 catttttgta acataactat tatatcgttg acacactgct atggtacaat ttttatatcg
    34321 ttaagaaaga cttaaatata tttagatatt tattaaatta agccagtcgg aggaatgaag
    34381 agtgaaaaag agtttaatat cagcagtaat ggtatgttca atgaccttga ctgccgtagc
    34441 atcaccgatc gctgccgcgg cggacgattt tgattctcag atacaacagc aagatcaaaa
    34501 gattgcagac ttgaaaaatc aacaagcaga tgcacaatca caaatcgatg ccttagaaag
    34561 ccaagtttct gaaatcaaca cacaagcaca agacctatta gcaaaacaag atacattgcg
    34621 tcaagaatct gcacaattag taaaagacat tgcagattta caagaacgta ttgaaaaacg
    34681 tgaagataca atccaaaaac aagcacgcga agcacaagta agcaacacaa gctcaaacta
    34741 catcgatgct gtattgaatg ctgattcatt agcagatgct attggacgcg ttcaagcaat
    34801 gacaacgatg gtaaaagcaa acaacgactt gatggaacaa caaaaacaag ataaaaaagc
    34861 tgtagaagac aaaaaagctg aaaacgatgc taaactaaaa gaattagctg aaaaccaagc
    34921 tgctttggaa tctcaaaaag gcgatctttt atctaaacaa gctgatttga acgtactgaa
    34981 aacttcatta gctgctgaac aagcaactgc tgaagacaag aaagctgact tgaaccgtca
    35041 aaaagcagaa gctgaagcag agcaagctcg tattcgtgaa caacaacgtc tagcagaaca
    35101 agctcgtcaa caagctgcac aagaaaaagc tgaaaaagaa gcacgcgaac aagcagaggc
    35161 tgaagcacaa gctactcaag cttcatctac tgcacaaagc tcagcaacag aagaatcatc
    35221 agctacacaa tcttcaatga ctgaagaatc atcatcagct acacaatctt cagcaacaga
    35281 agaatcaaca acacctgaat cttcaacaga agaatcaaca gcgcctgaat cttcagcaac
    35341 agaagaatca acaacagcgc ctgaatcttc agcaacagaa gaatcaacaa cagtgcctga
    35401 atcttcagca acagaagaat caacaacagt gcctgaatct tcaacaacag aagaatcaac
    35461 aacacctgcg cctacaacac catcaacaga tcaaagtgtt gatacaggaa acggcacagg
    35521 aagtagtact ccggctccaa cgccaacacc aacacctgaa caaccaaaac ctgtaacacc
    35581 tgctccagct ccttctggaa gtgtgaatgg tgcagcaatc gtagcagaag cttacaaata
    35641 tattggtact ccatatgttt ggggcggtaa agatccaagt ggctttgact gctcagggtt
    35701 cacacgctat gtttacatgc aagtaactgg tcgtgacatc ggtggttgga cagttcctca
    35761 agaatcagct ggaacaaaaa tttcagtttc tcaagcaaaa gctggagact tgttattctg
    35821 ggggtcacaa ggcggaactt accacgtagc gattgcttta ggcggaggac aatatatcca
    35881 tgctcctcaa ccaggcgaaa gcgtaaaagt tggttcagtt caatggtttg cccctgactt
    35941 tgctgtcagc atgtaagaat ga
//
LOCUS       DACCXW010000011        35364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCXW010000011 DACCXW010000000
VERSION     DACCXW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.011"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            61..360
                     /locus_tag="IVY71_000461"
     CDS             61..360
                     /locus_tag="IVY71_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7984536.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            625..1500
                     /locus_tag="IVY71_000462"
     CDS             625..1500
                     /locus_tag="IVY71_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984537.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1497..1715
                     /locus_tag="IVY71_000463"
     CDS             1497..1715
                     /locus_tag="IVY71_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984538.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1702..4329
                     /locus_tag="IVY71_000464"
     CDS             1702..4329
                     /locus_tag="IVY71_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984539.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4344..4562
                     /locus_tag="IVY71_000465"
     CDS             4344..4562
                     /locus_tag="IVY71_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984540.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            6081..6395
                     /locus_tag="IVY71_000466"
     CDS             6081..6395
                     /locus_tag="IVY71_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984541.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6448..7221)
                     /locus_tag="IVY71_000467"
     CDS             complement(6448..7221)
                     /locus_tag="IVY71_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984542.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            7326..7571
                     /locus_tag="IVY71_000468"
     CDS             7326..7571
                     /locus_tag="IVY71_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7984543.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            7727..8578
                     /locus_tag="IVY71_000469"
     CDS             7727..8578
                     /locus_tag="IVY71_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984544.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            8611..9285
                     /locus_tag="IVY71_000470"
                     /pseudo
     CDS             8611..9285
                     /locus_tag="IVY71_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9542..10741
                     /locus_tag="IVY71_000471"
     CDS             9542..10741
                     /locus_tag="IVY71_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7984545.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            10763..10975
                     /locus_tag="IVY71_000472"
     CDS             10763..10975
                     /locus_tag="IVY71_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7984546.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            11050..11526
                     /locus_tag="IVY71_000473"
     CDS             11050..11526
                     /locus_tag="IVY71_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7984547.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            11523..13331
                     /locus_tag="IVY71_000474"
     CDS             11523..13331
                     /locus_tag="IVY71_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7984548.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            13408..13623
                     /locus_tag="IVY71_000475"
     CDS             13408..13623
                     /locus_tag="IVY71_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7984549.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            13715..14578
                     /locus_tag="IVY71_000476"
     CDS             13715..14578
                     /locus_tag="IVY71_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984550.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            14609..15151
                     /locus_tag="IVY71_000477"
     CDS             14609..15151
                     /locus_tag="IVY71_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7984551.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            15165..15587
                     /locus_tag="IVY71_000478"
     CDS             15165..15587
                     /locus_tag="IVY71_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7984552.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            15517..17916
                     /locus_tag="IVY71_000479"
     CDS             15517..17916
                     /locus_tag="IVY71_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7984553.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            17948..19939
                     /locus_tag="IVY71_000480"
     CDS             17948..19939
                     /locus_tag="IVY71_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7984554.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            19962..20213
                     /locus_tag="IVY71_000481"
     CDS             19962..20213
                     /locus_tag="IVY71_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7984555.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            20203..21432
                     /locus_tag="IVY71_000482"
     CDS             20203..21432
                     /locus_tag="IVY71_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7984556.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            21429..23510
                     /gene="topB"
                     /locus_tag="IVY71_000483"
     CDS             21429..23510
                     /gene="topB"
                     /locus_tag="IVY71_000483"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7984557.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            23659..27579
                     /locus_tag="IVY71_000484"
     CDS             23659..27579
                     /locus_tag="IVY71_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7984558.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            27580..28524
                     /locus_tag="IVY71_000485"
     CDS             27580..28524
                     /locus_tag="IVY71_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984559.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            28528..28731
                     /locus_tag="IVY71_000486"
     CDS             28528..28731
                     /locus_tag="IVY71_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984560.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(29031..29267)
                     /locus_tag="IVY71_000487"
     CDS             complement(29031..29267)
                     /locus_tag="IVY71_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7984561.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(29450..29821)
                     /locus_tag="IVY71_000488"
     CDS             complement(29450..29821)
                     /locus_tag="IVY71_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984562.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(29818..31146)
                     /locus_tag="IVY71_000489"
     CDS             complement(29818..31146)
                     /locus_tag="IVY71_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7984563.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(31107..31436)
                     /locus_tag="IVY71_000490"
     CDS             complement(31107..31436)
                     /locus_tag="IVY71_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7984564.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(31694..32064)
                     /locus_tag="IVY71_000491"
                     /pseudo
     CDS             complement(31694..32064)
                     /locus_tag="IVY71_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            32209..32394
                     /locus_tag="IVY71_000492"
     CDS             32209..32394
                     /locus_tag="IVY71_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7984565.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            32906..33124
                     /locus_tag="IVY71_000493"
     CDS             32906..33124
                     /locus_tag="IVY71_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984566.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            33337..33996
                     /locus_tag="IVY71_000494"
     CDS             33337..33996
                     /locus_tag="IVY71_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7984567.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            33999..35342
                     /gene="vanS"
                     /locus_tag="IVY71_000495"
     CDS             33999..35342
                     /gene="vanS"
                     /locus_tag="IVY71_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7984568.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT        10019 a         8139 c         8869 g         8337 t
ORIGIN      
        1 tatttgtact tataaaggta gaggagttat agaataatta attaaaaagg agaggttttt
       61 gtggaagcaa taaaattaat gatttcagaa gaacaggagg aaatgtttct aaagcagttt
      121 tatcaaattg ctacaaaagc tattgaaaag gctcaagaaa atgcggggat gacaagggaa
      181 tatctcaatc aaaaagaaat ggcggaatac ttaaatgtga gtgaaaactt tttaaaaaat
      241 gctgtgagaa atgagggatt gcccgtagtt gaattagggg caagaaaatt ttattcgaaa
      301 aaggcagtta atgagtttat gtttgctcga atgaaaagag gggaaaatat tggaagatga
      361 tgaagtagaa attataaaaa aatgctttaa aaaaagaaaa aaagtgttcc caaaagtttg
      421 cagactgggg gaacactcaa taataaagtg ctgagaaaac tttatttata gctattatat
      481 caaaaaagaa ggtgaaaagg tgttttcttc tggaaatgtt gtagttgaca aacttagaga
      541 aataaatttt gacggaaatg taatccacac acatggtacg atcaattaag aaaaaaaacg
      601 aaaaaaggtg ttgagaagcc ctatttgaat gcaatagtta tcttagcaga aattacctat
      661 tggtatagac ctaaaaaaat ttttaacgat gatggtcagc tacttggtta tcaaaaaaag
      721 tttcttgaag atattttgca aaaatcatat aagcaattaa gcaaaaaaac aggattatca
      781 gaacgagcta taaaagatgc aattgtgtac ttagagaaaa aggggattat caaaagggtt
      841 tttagaaata tttattatga gaaaagagca gttacattat caaatgttat gtacattgtt
      901 ttaaatgtac ataaattgat agaaattact tttcctactt tggattgcga aaaaaaggag
      961 tatatgccga aaattcgtca gaggggtatc gaaaaaaaga gatatgtctc tttaaaaaaa
     1021 ggtagaggta tgtacaaatt tgtatatacg tatacaaaga ctacttcaca gaacatatca
     1081 aagactactc tcgttatgga agaagaggat attaatataa agaggattta cagtaagatt
     1141 agtgttcttt atgacgcaaa taacgaagat ggtcgaaaat tatttcaagt gtatcactat
     1201 ttgagaaacg ctaatgtaga tacagaagat atcagaaaaa tcattgaata tttatctgaa
     1261 aatactcagt atcttattgc tgagtgtatt ataaaacaac atgaagcatg cttttttcaa
     1321 agaaaaactg aagaagggtt atacgattat gtaacatatt ttttgaatgg acttcagaaa
     1381 agatataaaa gtcaattttt agaaaatgta gagcctatag atcagttcaa aaaagctttc
     1441 gaaatcaaag agttacctga aattactatg cataattggt tgaaaggaga agaatcatga
     1501 ttaaaaatcg cttaatagat caaatgtggt gtcttgatca aaatacagat actctcgaat
     1561 taatcgagag tatctgcttc aacacagtag ttctcgattt aagagctgaa aatgacaatt
     1621 gtgtaactca tatcataatt aatcaaaaaa tggctcagca gctctctgag agcctaagaa
     1681 aatgggctga aggagaaaac tatgaaagca attaaagaaa aaatcgttag tagaaaaata
     1741 aactggttag cactcagtgt acttgtttta atgattagcg ttgtttttac aagtaatatt
     1801 gttgaagcta gcattttcaa ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat
     1861 aattctcttt atagacctta cctaaaaaaa tacaatggag atttccttag tcagttcggg
     1921 atttggcttg gatgggctgt ggtaaaaggc atgtttactg tgactgattc gattcaaaat
     1981 atgattccag atgtgttgga tttgttcaat ttcattgaaa gtacagggct aaataatgtt
     2041 tatcaatcag tcataaatac aattgtcgtt ggattaatga tcctttcatt gatgtttgtt
     2101 ggctacaaga tgattactgg aaaaggaaca atagatttaa gatctgttgg aatgaatata
     2161 gtgatgtctg tagcgttaat tcttttaatg ccaacaatga tttctagcgg tattcaattt
     2221 gccaaaatat tttacaatga tgctactacg attacaaatt cagatgacgg agtcgcttgg
     2281 tcattgataa aacaaggagt aacagatctg gcatatatta ataaaacaga tcaatactca
     2341 agcattgata aaacggagga tcgaaataag ttaacacgaa aaaattttca acagacagat
     2401 ttaacacaag tgttgacgga taaagtgatt gataaattgg aaaaagaaaa tccagctgcg
     2461 gataatcttc gctatgaatt agttgaaaat tctaataatg aatttgttgc cacaaagttt
     2521 tctgataact tcttatcaac attttctgat agcttgaaat caggttatta tcgatatcaa
     2581 gcaaatcttt gggggatttc gattgggttg acagcgttgg caatcgccta tgtgttcagc
     2641 gcgtttgtga tcattacagc aatattagaa cttgcattta aacgtgtgtt aggtgttctt
     2701 gtttttgcga cagatattga aacaggacaa cgttcaaaag ttgttttatc cgatatttta
     2761 caatgttact taacagttgg atttcaaggg tttggactat ctatgtttgc tatgtttatc
     2821 aattttttaa atgctggaca aggtatctcc acaaatattt ttataaaaac aattgcttat
     2881 atctgtgctg tgtttgtgct gataaaaggc agtggaacag tcatgagata ctttggtgtt
     2941 gatattggac tcaaagaagg ctatgggcaa cttgcttctg cttttggtat gggcgcaatg
     3001 ctgtttagaa aaggatcaac tggatttaat cgtgctaaag gaagcggaaa tggaaatgga
     3061 tcagacagtg gagaaggaga agatcgaaaa ccagaaaaga atttcggcga aactctatca
     3121 aaaaaagctg gcaaaactgg aagagctttg ggatatgccc atgaacgtgg attatcaggt
     3181 ttagcttcag atggagctac aatggctagt gaaagagcaa taaaaccatt taagagtatg
     3241 cgtgatatgg ctaatgatac caagaataag ttcaaagaag gtttagatga tggtacagtg
     3301 tccgctatta ataaaaatag caaacctatg ttagctaaaa ataaagaaga tgaaactgga
     3361 aaatatgctg attccatgcc aacacgtctt tcggatagaa aagatggtgc taaggttgaa
     3421 aatgccgata gaattatgtc tagttctgaa cgtatgcgtg aagcaatgaa aaataatgct
     3481 gaatcgaata ataatcctgt aagtgcaatt cagcaaaaag ttcaacaaga tattgaagaa
     3541 cgtaaaaatg ctatgcacgg tcaagctaag tctgctgaag aattaattaa tcagaagcga
     3601 caagaagcta aatacacgcc tgaagcaatg aatcgtgagg aaatgttaag gaaacgtgta
     3661 gaagggcgta caggagctaa tacggacgaa aaagaagcgt tgaccaaaga aagaatccaa
     3721 gaagctaaaa actcaaatac cggattagaa aaattggtca aagaaaatct acaaaattct
     3781 acaagtagtc aaggtggccg atcagtagat gtccgagaaa atattcaagg ttcattgaat
     3841 ggtcatgtcg gacggaatgt tgatgtaaga gagaatttac aaagctcttc aaatggtcaa
     3901 ggtactaaga caattgatgt tcgagaaaat gtacaaaatt ctacaagtag tcaagggttt
     3961 aaatcaatgg atgtaagaga gaatgtacaa aattctacaa gtagtcaagg gtttaaatca
     4021 atggatgtaa gagagaatgt acaaagttct acaagtagtc aaggattcaa aacagtagat
     4081 gttcgggaga acgttcaaaa agatagtgga gaaatgaaag aaaaaacaca aaaaatcaat
     4141 atcgttgaag acagaaagtc agcgaaaaaa tttgattcaa atcatgaaac agtgattatt
     4201 gatagtgaaa tccgtgaaaa tgataaggga tcaaaaccaa gacgaagatt tacctatgaa
     4261 gacaacgagt tattcagaga tacacttaat gatcctaatc cgcttttcga taatctatta
     4321 aaaaaataaa agggggaatt gtcatgaaat taattctaaa gattagtacg catgatttct
     4381 tagaacttga cgaattatgt gaatcagtaa agaacgcaac aaatgaaaaa atcacggtca
     4441 ctaattatat tgaactgatg attcaagaag aattaaagat gaaacatgag ataaaaaaca
     4501 acttgaaata ttgttatcta gaaaaaagtg aagagataat ccatgaatta aatggctttt
     4561 aaaaattgta tttgacatga atttacgaca aggaattagt ggggattaag cgattattct
     4621 cttccacttt agagaaataa tgggttaaaa agccagaata cgtagagcaa tattgacaac
     4681 ctataaagga gctattgttg aaaattgaag taacaaaaag ataaagagag gtttacatct
     4741 atcagattaa aagtgatagt acacccatga aacctgcaaa agagttgaac ctaatgtata
     4801 tcggtttaac tctttttatt tgcaaattaa aaaaatatca ccgaagtgac gaatgaaact
     4861 atgaccaaat taagcaagaa aatatatctt gcaaataaag tagtataaaa agatattgat
     4921 aaagccaatc aaaactttga atttcaaacg ttagataatc aaacagatac aaaaaatgaa
     4981 atcagttcta cgcgtacgat tattaaaaat aataaatttt cactagaaga aaatggaaat
     5041 aaaatcaaat aattaggaag aaaaaacaaa gcaattagaa aagcctgcaa gcaaatttga
     5101 gtaatcgaga tccaattttt ttaaggaagt ttaacaaaaa tatgtaattc tattatcact
     5161 attaaagaga aagttggagc ggaatataaa gaaaagagaa aagtaattaa acgcaaaaaa
     5221 atgtagaact taaaaaatat aaagaggaac aaccgttaaa attaggaatg atgttgtgta
     5281 aacattataa ttgctatgat ataaatgaag tgttaaagaa agcaaattta aaccaatttt
     5341 gtgaacagaa aagccttagt cactatgacc aaaactacgg acgaaactat gaccaaaagt
     5401 aggtactaaa ttatgaccga aaataggtcc aaaatggatt tttttatgat atcaaaaatt
     5461 atgaaatgat tttgaaagaa aaccagttgg cacagccaac ttagccacct tcctgtagga
     5521 aggtgctatc agttttccct tatgctcttt tctttctttt ttgtttcata tgtcaaatgc
     5581 cattttgaaa tgtaccatct taagaaattt atccttaaaa acaattgcgt tattggataa
     5641 aaagatgcta cattatctta aaaaaggaat caaatatgat gaatttagtg gctagcatat
     5701 cgtgtgatta agatacttta tagtgtaagt tgaaggagaa aaaatattta aaatatactt
     5761 cccatttcag tgggaagtaa aattaatctt gttgtttcta ctagtaatgt atctttttgg
     5821 aaactatgta acgatgtaaa caagattttt ttcagaagaa aaaacgataa aagttgtgat
     5881 aaacgattta aaaaatagga gaaaatatat caagcgcatg gtatgaaacg cacatttaaa
     5941 aagattcgac taactaaaaa aataagttta caaagcattg ttgtgtatta caagtgatct
     6001 atacactgaa aaatgataga gaaaatctga tacgtccgca acaatggaca attgaaaatg
     6061 aaagtgagga aattggaaga atggctgatg aaaataaagt tgttccgctt gaaacactag
     6121 tgataaataa ggaagagttt gagcaacttt ttcttgattt ttttgttcaa tatgacagga
     6181 tttatgattt tagttcgtca ctagatttat ttgaattaga ggcagaaata atagaaaaca
     6241 gtcgagagga gaatggtaaa atcatcattt cagtagaaaa attcaatgaa ttattctcag
     6301 aaacatttga aatagataca gattattatc aaccacaaga agttttccaa tcatttagaa
     6361 atcagattat tcgctcaggt aagaccgtaa actgaaaaac aataaaagaa acgttgaaag
     6421 aataattatt ccttcaacgt ttctttttta gaatgtttta atgaccaaag cagtttcatt
     6481 gtcaggctta ggtaattcat gaaatcccgt atttatagtg ctcaattgat caactaacca
     6541 tgttttactt tttgaattgt agataaatct aaaattaata tcgttattat cgtgttgaag
     6601 gggcgtctcc aaataatctg ctgtatattc atagtcaacg taacctgcta atacaaaaga
     6661 gagtatccct ttatttaatg ttagatttaa tggataatca tacgacttta tttttttcaa
     6721 cttcatattg tacatatctt cagccataaa agttgagttc gtgatttcat cttgggtctc
     6781 attaaaaaag ttatccgaat aatcgcttat ttttgaaaag tctttattgg caccagcctc
     6841 aaaaaattgt tcaacttttt cagtataaag ttgtgctaac gctccttttt caatattaac
     6901 tttaatattt tcatgtctat caatttgtgt gctgttcagt aagggaagat cagcttttag
     6961 ttttatatcg tcttgatttc tttcaatttc aattggcaaa atcttttcag tagattcagt
     7021 ttctgataaa ggaacatcta gttttttatc atgataataa gcttcaaatc catctggaac
     7081 cggaaaaaaa tgtattgggt aattcttttc tgaggttgtt gtactagttt ccgtacttgt
     7141 agaacttgtg tcagaagtgg tacacccgct tatagttccg atgagtaaag cgcaagttag
     7201 aatgattgca aatttcttca aaaaaattcc tccttaaaat tgaatatgga ctaatactag
     7261 tcccattgta gcataccgaa tctttaagat taataagaaa aaggactaat tttagtccaa
     7321 aaggtgtgct attcttgaaa gaggaaaaaa ttattgataa agaaaatgta atcggtagca
     7381 atattagacg agtccgatta gaaaaaggaa ttggtcaaac cgagctgatt agagacctac
     7441 agcttcgtaa aattgctata actagggaaa cgttggttaa gattgaaggt ggccgccaac
     7501 acattaagtt ggatcaattg aaagccatca aagatattct acaggtgtct tatgaagatt
     7561 tgcttcaatg actacttgta gtcatttcgt ttgaaattat tatgttacac ccttatgtgt
     7621 cacgaagaaa tactggtata ataatgaatg ggatatgtaa cgatagggta tactgttatg
     7681 ttgcctaata aattgtggat caaaatacaa gaatggaggg atatccttgt ataaggcata
     7741 tgaattggat tttacaaatg ctgacttaaa tgcattctcc tcaagctcgc aatcatatac
     7801 acatgtagta aatcaaatag atcacaacca aaaatctatt aacaagagaa tagaaaatct
     7861 atttgaaagt gaaaatgatt tagttgatta ttattctaag gacagcaaaa ttctagatgc
     7921 agacgaaatt attaaagatt ggtttccaac tataaaagca gatattttta tatctcactc
     7981 tcatgctgat gaaaaattag ctatacgttt tgcatcatgg ctatttgaaa attttggtct
     8041 tactgcattt attgattctt cggtttgggg atacagttct gatttattaa aaaaaataga
     8101 ccaaaaatat tgctataagg aacaaacaaa aacatatgat tatgataaaa gaaatgtaac
     8161 aactagtcat gttcatatga tgctttctac agctttaaat aatatgattg atagtacaga
     8221 atgtctgttt tttcttaata cacctaattc aattagtttg agcaatgaaa tcactaatga
     8281 acagaaattt acgtattcgc catggctata tagcgaactg acgacagctt caatagtgga
     8341 aaaaaagaat ccacgattgg aaagtaatcc tcaaatgagc accgaggatg ttagaagcat
     8401 tataaagcac tattcttcgc aagaaaatct gcaaataaaa tattctttaa atttaagtga
     8461 attaacttat ttacgaaaag ctgatttgaa aaaatgggct ttagatgctg tggaatgcac
     8521 taaaggaagt aatacattag atattttata taaaaacacc attttcacta atctctaatc
     8581 aatttactga atcgaaagga accgggtaga atggataaga aagttttaat aaagaggttc
     8641 tctgaagcta ttgaaaaagg aaatgcagca atatttgcag gtgcaggatt atcgatgtca
     8701 caaggatatg taagttggcc ggaactactt aatgatcctg caacagaaat cgggttggat
     8761 tctaaaaaag aaacagactt agttactctg gctcaatatt ataaaaatga aaatggtgga
     8821 tcaagaggaa ttttgaatca gatacttatg gataattttg gtgaagaatt agaaatatct
     8881 gaaaatcatc gaatactagc aagtttacct attgaaactt attggacaac taattatgat
     8941 catctaatag aaaaatcaat ccgagaagct tataaaaacc ctcaggttaa gaaaaattat
     9001 actcagttag caacaactaa tccaaatgtg gatacaattg tttataaaat gcatggtgat
     9061 atagatgatg tcagttcgac agtaataaca agagatgatt atgaaaaata tgatgattcg
     9121 tacgcgttat ttaaagaaac tttaaaagga gatttgttaa caaaaacttt tctcttttta
     9181 ggatttagtt ttacagaccc taatttagaa agaattttat cagatataag atgggttttg
     9241 agagaaaacc aaagacctca ttattgtata atgagaaaaa tattatagaa aaaattttag
     9301 gttatcgctg aaagccccgg aaatacgggc atttcgcagg atatgagaac tcaatttact
     9361 accaatttac tactttcgga ttgagccgca tatatgctga accatactgc taatacccgc
     9421 caccccaaat acggcacatc ataaattgaa tacgacaccg taaacgcggc gttcctcttt
     9481 cccgtcatgg gagaacagga cgccgctttt ttatgcccaa atagagaaag gaggcgacgc
     9541 catgctattt acttccggcc ctgaccgggc gtttgaacgg ctgatgcagg aaaagcccgg
     9601 tttcgatcac tacgacggcg gcggggcgga cgcgccggag gattgcggca cctgccgatt
     9661 ttaccgcccg gattggaaat atcaattctg ccagtttgca gagtgtccct atcagccggg
     9721 caagctgacc gactatgcag cggtcaaatt cacagtgaaa ggagcgaaca acgctatggc
     9781 agttttccgt gtggaaaaga acaaaggcta tacggtgatg tcaaatcacc acctgcggaa
     9841 taagaacctg accttaaagg ccaagggcct gctgtcgcag atgttgtcct tgccggagga
     9901 ttgggactat accctaaaag gactgtccta tatcaaccgg gaaagcatcg acgcgatcag
     9961 gacagcggta tgggagcttg aaaaagctgg atatatccgg cgtgagcagg gccgtgacgc
    10021 aaaaggcaaa atggccgata tggtctatac catctacgaa cagccggtat tggaaaaccc
    10081 ggtattggaa aatccaacat cggataaccc ggtgttggaa aatccgacat cggataatcc
    10141 ggcgtcggaa aatccaacgc aattaaataa agaagtacaa agaactgact tatcaaaaaa
    10201 agaaaaaata attacagatg gacaaagtac ccattccatt cctatcccat ccccgacccc
    10261 tgcccctatg gagggcgagg cagcggctac gccgccggaa cggaaaagga caggaaagga
    10321 tgaggctgta cggatttatc gggaaatcat tttggagaac atcgagtatg cctatctcat
    10381 tcaggacagg agcattgacc gtgaacagct tgacgaaatc gtagacctga tgctggaaac
    10441 cgtctgcacc gcccgaaaga caatccgtgt cgccggggac gactaccctg ccgagctggt
    10501 gaaatccaag tacatgaagc tgaccggcga acatatccgc tttgtccttg actgtctgcg
    10561 ggagaacaca acgaaagtcc gcaatatcaa acaatacctg cgggctatgc tgttcaacgc
    10621 ccccagcacc atttccaact actacacggc ccttgtcgcg cacgacatgg cacagcccga
    10681 ttggggaaag cctaaatccg gcctgccgga ctattcctgc ggcgagggcg aaagcctgta
    10741 acgagaaaag gaggacgacc ctatgaagca gggcgcattg atttttgacg agcgaacaga
    10801 ccgttacgac atccgctttg atctggccga ctattacggc ggcctgcatt gcggggagtg
    10861 tttcgacgtg atgatcggcg gcaaatggag gcccacccgc attgaaatgg ccgaaaactg
    10921 gtatcttgtc ggcatccggg ccgacgacct ttccggcctg cgggtgcgga tttgacctat
    10981 atctacctgc ggcgtctggt ggttccgcct tagacgccgc ttttgacttt ctgacaagga
    11041 gggatgcgat tgcaggaaga agttactcaa aagaccattg ccctgtccat gaaaacgggc
    11101 aagctcacgg ctcaagtgct gcaagcggcg ctgaaaaagt acctgcaaca ccgggccaag
    11161 ggcaaaaaca cgctgcacca cggccagcag agcctaaagc agcttaaaaa gcatggggct
    11221 gatctctcca acattgagat caccgaggcc aacattggag cgttcaagcc atgcgccaag
    11281 aaatacggcg tggattttac cttacgaaaa gataagacca cccagccgcc ccactacgtt
    11341 gtgattttca aggccaagga tgcagataac cttgatctgg cgtttcggga gtttaccgcc
    11401 aagacgcttt ccaaggagca gcggccctct atccgcaagg ttttgtccgc tgtgaagcaa
    11461 aaggcggcgg cccagaccaa gcagcgggcc aaggagaaaa tcaaggaaag ggggctggaa
    11521 ctgtgaagcc tgacgtaaag aaattgctga tcctgaacct cccgtatctg atttttgtgt
    11581 acctgtttgc gaaatgcggg gaggcgtacc ggctggccgc tggtgctgac ctgtcgggga
    11641 agctcctgca ttttgcggat ggttttgcgg cggcttttgc caacccgctg ccaagcctgc
    11701 acctgtttga cctgtgtatc ggtatcgccg gtgccgtgat cgtgcggctg gtggtgtact
    11761 gcaagagcaa gaacgtgaag aaataccgca aaggcatgga gtatggcagc gcccgctggg
    11821 ggacggccaa agacatagcg ccttacatcg accccaagtt tgaaaataat atcctgctca
    11881 cccaaacgga acggcttacc atgaccggca ggcccaaaga ccccaagaca gcgcggaata
    11941 aaaacgtgct ggtgatcggt ggcagcggtt cagggaaaac gagatttttc gtgaaaccca
    12001 acctgatgca atgctttccg accacggact atccgacttc gtttgtggtg acagatccga
    12061 aaggcacttt ggttctggaa accggcagga tgttccagca ggcgggctac cgcattaaaa
    12121 tcctgaatac cattaacttt tccaagtcca tgaagtacaa cccctttgtc tacatccatt
    12181 cggaaaagga cgtgctaaag ctggtaaata ccctgatttc caacaccaag ggcgaggggg
    12241 aaaagtcagc ggaggatttt tgggtgaagt cggaacggtt gttttattcg gccctgatcg
    12301 gctatatctg gtacgaggcc ccagcggagg aaatgaactt ctcaacgctg ctggaaatga
    12361 taaacgccag tgaagcccgc gaggacgacc cggagtttca aagccccgtc gatctcatgt
    12421 ttgagcgttt ggagcagaaa gaccccgacc attttgcggt gcgccagtac aagaaattcc
    12481 tgctgtccgc aggcaagacc cgttcttcca tcctgatttc atgcggtgcc agacttgcgc
    12541 cctttgacat ccgggaggta cgcgagctga tggaggacga cgaaatggag ctggacacca
    12601 tcggggacga aaagacggtg ctgtatctca ttatgagcga tacggacacg acctttaatt
    12661 tcattctggc tatgctccaa agccagctaa tcaacctgct ttgtgaccgg gctgatgatg
    12721 tgtacggcgg gcggctgcct gtccatgtac gcctgatttt ggatgaattt gccaacattg
    12781 gccaaatacc caatttcgat aaactcattg ccaccattcg aagccgtgaa atatcggcct
    12841 ctattatttt gcagagccag tcgcagctaa aagcgattta caaggacgcc gccgagatca
    12901 tttcggataa ctgcgatagt gtgcttttcc ttagtgggcg cggcaaaaat gccaaggaga
    12961 tttccgatgc gctggggaaa gaaacgattg acagtttcaa caccagcgag aaccggggaa
    13021 gccaaacgtc ccacggattg aactatcaaa aattaggaaa ggagttgatg tcccaagacg
    13081 aaattgccgt gatggacggc ggcaagtgta tcttgcagct acggggtgtg cgcccgtttt
    13141 tatcggagaa attcgatatt accaagcatc cccggtacaa ataccttgcc gacgccgaca
    13201 agaagaatac ctttgacgtg gacagatttt tgactatcac gcgccggaaa cggcagcaag
    13261 tggtaacgca ggatgaagta ttcgacctgt atgaaatcga cctgtcggac gaggacgccg
    13321 ccgctgaata aacagcggct tttttcattc caacaacaaa atcaaaaaat cattatgagc
    13381 cggaaaccgg cagaaagagg taaatttatg gcatttttta attccgcagt tactactttg
    13441 cagactatcg tgatcggact gggcggcgct ctgtgcgtat ggggcggcat caatctgttg
    13501 gagggatacg ggcaggacaa tccgggggca aaatcgcagg gtgtcaagca gcttgtcgct
    13561 ggcggcggcg tggccctgat cggtgtcact ctggttcccc tgctgtccgg gctgctgggc
    13621 tgatcctggc ctttgtccct gtccaaaccc ttaactaatc gcctccgcgt cctcccggcc
    13681 agaccgggag ggcgcgggaa aggaggtgct acccgtgatt agtgaaatca ttgaagaatg
    13741 gataaaaggc atcctcatgg acggtatcac gggcaacctg tccgggctgt tcgatacggt
    13801 aaatgccaaa gtcggagaaa tcgccgccga cgtgggacga accccgcagg catggaacag
    13861 cggtattttc aatatgctgc acagcctgtc ggaaaccgtg atcgtcccca tagccggggc
    13921 catactcgcc cttgtcatgt gctatgagct tatccagatg ataacggaaa agaacaatat
    13981 gcacgacttt gacagctcca tgttcttccg ctggatattc aaatccgcgt ttgcaatcct
    14041 gatcgtaacg aacacatgga acatcgtcat gggcgtattc gacgccaccc aaagcgtcgt
    14101 caatcaaagc gccggtgtga ttatcgggca gacatccatt gattttaaca ccctgatacc
    14161 agaccttgaa agccaattgg aggctatgag cgtgggcgct cttttggggc tgtggttcca
    14221 aacccttgtt gtgggcctca ccatgaatat cctttccatc tgcattttcc ttgtgaccta
    14281 tggccgcatg gtggaaatct atgttgtcac gtcgctgggg cctatccccc ttgccacaat
    14341 gggcaacgcg gaatggcgcg gcatgggcca agggtatctg aaatccctgc tggcgctggg
    14401 cttccaagcc tttttaatca tggttgtggt gggtatctac gcggtgctga tacagaatat
    14461 cggttccgct tccgacatat ccggcgcaat ctggggctgt atgggctata cggtgctgct
    14521 gtgcttctgc ctgttcaaga ccggcagtat atcaaaggcc gtattcgggg cgcattgatc
    14581 ccaaaatcca agcatacaca ggaggaactt gtttatttca acgtacaaca tcatttacgc
    14641 tgacccgccg tggcggtatg actgcaaaaa cgtacagggc gcagcggaaa atcactattc
    14701 caccatgagc attgacgagc tgtgcgccct gccggtggaa cggctggcgt ccaaagactg
    14761 cctgctgttt ttgtgggcca cattccccat gctgccggaa gctctgcggt taatcaaagc
    14821 gtggggcttt tcattcaaga ccgtggcttt tgtatggctg aaacagaacc gtaaaagcct
    14881 tacatggttt tacgggctgg gccgctggac aaggggaaac gcggaaatct gcctgctggc
    14941 aacacacggc aagccgaagc gtcgttctgc ctctgtccat cagtttatta tcagcccgat
    15001 tgaacaacac agcaaaaagc cggatgtgac ccgtgaaaaa atcgtagagc tggcgggcga
    15061 tttgccccgc gtggagctgt ttgccagaca gaaaaccccc ggctgggacg tgtgggggaa
    15121 cgaggtggaa tgtgacctta tcctgacgtg aaaggaggtg ccaaatggct tatgtaaccg
    15181 tccccaagga cttaacccat gtgaaaagca aggttgtgtt tgggcttacc aaacggcaac
    15241 tgatctgttt ttccggggcg ctggtgatcg gcgtcccctt atattttctc acccgtgact
    15301 atcttaccaa cagcgcggcg gccctgctca tggtatttgc catgctgccg ggctttttgc
    15361 ttgccatgta cgagcgtcac ggccagcccc ttgaagtggt gattatgcag atcatccagt
    15421 gctgcttcat ccagccaaag gagcgtccct atcagaccga caacacctac accgcccttg
    15481 tgcggcaata ccagatggaa caggaggtaa aggccattgt ccaaaaagga aatcccaaga
    15541 gaaaccgaaa aaacaaagct gacccgcgcc cagcgaaaag aaattgacgc ggtaatccgt
    15601 aaatataagg gcgacggcag gccccacacg gcgcagcaga gcatccccta cgaggtcatg
    15661 tacccggacg gcgtgtgccg ggtatcgccc ggcgtctttt ccaagtgcat tgagtttgcg
    15721 gacatcagct atcagcttgc ccagccggac acgcagacgg ctatctttga aaaactgtgc
    15781 gacctgtata actatgtgga cgcctccatt catatccagt tttctttcct caatcggaaa
    15841 gttgacccgg tgcagtacgc caagagcttt gagatcgcgc cgcaggggga cgacttcgac
    15901 gacatccggg ccgagtacac cggcattctg caaaagcagc ttgcaaacgg caacaacggc
    15961 atggtaaaga ccaaatacct gacattcacc atcgaggccg aaagcgtgaa agcggcgcga
    16021 gccagattga agcgcatcgg ctttgacctg ttaggctatt tcaagagcat gggggccgta
    16081 gcccatgtga tggacggctg ggaacggctg aacctgctgc atggcgtgta ccacccggac
    16141 ggcgagatat tcaattttga ctggaagtgg ctggccccgt ccggcctctc caccaaggat
    16201 tttattgccc cgtcctccct gtgcttcggc aatgcaaaga cattcggcat gggcggcaaa
    16261 tacggcgcgg taagtttcct gcaaatcctg tccccggagc tgtcggacga tatgctggcc
    16321 gacttcctca atacggaaag cggtgtttta gtcaatctcc atgtgcaggc cattgaacag
    16381 accaaggcga tcaagaccat caagcgcaaa attaccgacc ttgacgctat gaagattgcg
    16441 gagcagaaaa aggccgtccg cagcggttac gatatggaca ttcttcccag cgaccttgcc
    16501 acctacggcg aggacgccaa gaagctgctg acaaagttgc agacccgcaa tgaacggctg
    16561 ttccagttga cgttccttgt cctcaacgtg gccgacacca agcaaaagct gaataacgac
    16621 gtgttccaag ccgcaggcgt ggcccaaaag cataactgtc cccttgtccg gctggactac
    16681 cagcaggaac agggccttgc aagcagcctg ccgctgggcg tcaatcagat caagatacag
    16741 cgcagcctta cgacttccag cgtggccgtg ttcgtcccct ttgtgacgca ggagcttttt
    16801 cagggcggcg cggccatgta ttacggcatc aacgccaagt cccgcaacat gattatgctg
    16861 gacaggaagc aggcccgttg ccccaatgcc ttgaagctcg gcacccccgg cagcggcaaa
    16921 tccatgagct gtaaatccga gatcgtcagt gtgttcttaa cgacgcctga cgatattttt
    16981 atttcagacc cggaggccga atattacccc cttgtcaaac ggctccacgg acaggtaatc
    17041 cggctgtcgc ctaccagcaa ggactttgtg aacccgctgg acatcaacct gaattattcg
    17101 gaggacgata acccgctggc cctgaaatcc gactttgtgc tgtcgttctg tgagcttgtc
    17161 atgggcggca agaacgggct ggaagccatt gaaaagaccg tgatagaccg ggctgtgcgc
    17221 gtgatctacc ggccctatct ggcagacccc cgcccggaga atatgccgat cctgtccgac
    17281 ctccacaaag cgttgttaga tcagcatgtc ccggaggctg accgggtggc gcaggccctt
    17341 gacctgtatg tgtccggctc cctgaacgta tttaaccacc gtaccaacgt ggacatcggc
    17401 aaccggcttg tgtcctttga cattaaggag ctgggcaagc agctcaaaaa gctggggatg
    17461 ctcattgtcc aagaccagat atgggggcgc gtcacggcca accgaagcca aggaaaggcc
    17521 acatggtatt ttgccgacga gttccatttg cttttgaaag aggaacagac ggcggcctat
    17581 tctgcggaga tttggaagcg gtttaggaaa tggggcggca ttccaacagg tgccacccag
    17641 aacgtcaaag acctgctttc ctccccggaa attgaaaata tccttgaaaa cagcgacttc
    17701 attacgctgc tcaatcaggc gtccggggat agaaaaatcc ttgcggaacg cttgaatttg
    17761 tccaccgaac agcagaagta catcgacaat tccgaacccg gcgagggcct tttgatcttt
    17821 gaaaacgtgg tgctgccctt taccaacccc atcccgcaca acacccagct ttacaagatt
    17881 atgacgaccc gcttaaacga ggtggcggga gtatgacccg gcgacgccac aaaggaggtg
    17941 aaccaccatg aaagagctga aagccaaggc gaaagtgacg caggccatga cccgcgacgg
    18001 gctgacgctg gaaaatcagg ccaccggcga ggtggagaac atttccagcc gggaggccga
    18061 acaggatttg tccgcgccgg ggccgggcgg caccgccgat aagctgctgg aacgggccga
    18121 cgccatccat gacggccata aggccaagaa agccgcgaag caggccgggg ctgtggccgc
    18181 cgagggggac gctgccctgc accggccatc cgcccggctc caactgtcgg aggaagaacg
    18241 ggccgacccg gatttacagc cctatatccg aaaatcggac aaacgggccg ataagctgga
    18301 taaggcccgc gccgccatcc ccaagaagcg cgtcccggtc aaggaaaccg tctttgacga
    18361 ggcaagcggc aaagcaaaga ccgtcctgca ctttgacaaa caggacaagg ggcctccctc
    18421 cctgaaaccc aatccggcag gccgtcctgt ttccgaggtt tttctattcg gccacgggaa
    18481 aatccatgaa gtcgagcatg aaaacgtggg tgtggagggc ggccataagg gggaggaact
    18541 tgccgagcgg caggccggaa aagccatccg cagcaccatc cggcaccaca ggctgaaacc
    18601 ctactgggac gcggaaaagg ccgagcgcag gctattgtct gccaacgcgg agtatttcta
    18661 ccagaaatct ttgcgggata acccggagat cgcacaggcg gccagcaacc ccatttcccg
    18721 gttctggcag aaacagcaga tcaagcgcca gtacgccaag gccgcccgga cagcgggcca
    18781 gaccgcaggc gcggcggcac agggcgcggc caagacagcc ggggcctcca cgaaagcggc
    18841 caaggccgca gccaaagaaa gtgaacgggc ggcctccttt gtggcccgcc actggaaagg
    18901 ggcgctggcc gtccttgtgt tggggctttt gatgctgctt atcatcggcg gcttacagtc
    18961 ctgcaccgcc atgttcggca gcgccggaag cggcatcacg gcgtcctctt acttttccga
    19021 ggatagcgat atgttggccg ccgaggacgc ctacgcggag ctggaagccg agctgcaaga
    19081 ctatctggac aactacgagg ccacccatga ctatgacgaa taccattttg accttgacga
    19141 gatcagccat gacccctatg tgctgatttc cattctgtcg gcgctccatg acgggatatt
    19201 taccatcggg gaggtacagg gtgatttgtc catgctcttt gacaagcaat atatcctgac
    19261 agaaaacgtc gtggtagaaa cccgttaccg caccgaaacc gatacatgga cggacgcgga
    19321 gggcaacacc catacagaca cctaccaagt gccgtatgac tattacatct gctacgtcac
    19381 gctggaaaac ttcgacctgt cccatgtgcc agtctatatc atggacgagg aaacgctaag
    19441 tttctattcc ctgtatatgt ccacgctagg aaaccggcct gatctgtttt ccggcagcca
    19501 gtaccccaac gcctccaccc tgacgccgcc gacctactac gacgtacccc cggaggcttt
    19561 ggaggatgcc acctttgccg ccatgctcaa agaggcggaa aaatatatcg gttatcccta
    19621 tgtgtggggc ggcagctctc ccagcaccag ctttgattgc agcggcttca tttcgtgggt
    19681 aatcaatcat agtggatgga acgtgggacg gctgggggcg cagggcctat ataacatctg
    19741 cacccctgtt tccagttcgc aggccaagcc cggcgacctt gtatttttca agggaaccta
    19801 cgacaccccc ggcgtgtccc acgtcggcct gtatgtgggc aacaatgtga tgctgcattg
    19861 cgggaacccg atttcctaca cgaaccttac ttcaaactac tggcaatccc atttttacag
    19921 ttacgggcgt ttaccttaac gccagaaagg agctttcaat tatggcaacc aaacttgacc
    19981 gcatcgaaaa ggacatccag aaaaccaagt ctaagatcgc ggagtttcag aaacagcttc
    20041 gggagctgga aacgcagaaa accgaacagg agaatttgca gatcatccag cttgtgcgcg
    20101 gcatgaacat gaagccggag gaatttgccg cgttcttacg cagcggcgca atgcaggccg
    20161 cccctgccgc aaccccttac catgaacagg aggacaatgc ccatgaagaa taagaccaga
    20221 cggatttccc gtattctccc cgcacttctg gccgtgatgc tctgcgtcac ggcctttccc
    20281 gttaccgccc ttgcaggcgg caatgacccg gcaccggccc cgctgccgga ggctacaacc
    20341 gaacccgcta cgggcggttt ggagcctgaa acagacgaaa ccggcaccgc cacggacggg
    20401 acagaaaccg aggccgaaaa agataccggc cctctgaccg gcaaggacat caccgacctg
    20461 ttttccgcgc tgttcggttc caaggtgagc attgccgcca cggacgacgg catccagatc
    20521 accaccggca ccgacaaaaa agaacctgaa cagaccggca ccgtcaccac caacggcggc
    20581 aggctgaatg tccgcaccgg cgcggggctt gacaagaccg cgttcaccca gcttcccaat
    20641 gggacgacgg ttgaggtagt cggcacagac ggcgactgga ttaaaattct cttgccggag
    20701 cgcatcggct atgtccattc cgactatatg accgtcagcg aaaaggaagt agcggctacc
    20761 ggcgagggcg gtttttccct ctccattgac ccggaggaaa ttgcttctct gctggaactg
    20821 ttcaatggcg gcggggccag cgccgccctg tccccggacg ggaacctgtc actgattgac
    20881 gacatcggca gcgccgcgaa aaatggcaag cagtttatca ccgtggaaac caaaaacggc
    20941 aatgtgtttt atctgattat tgaccgggac gacgagggaa aagaaactgt ccatttctta
    21001 aatcaggtgg atgaagccga tctgctgacg ctgatggagg acggggaaac cgtcaccccc
    21061 gctgctgtct gctcctgtac cacgaaatgc aaggccgggg ctgtgaacac gaattgcccg
    21121 gtatgtaaaa ccaacctgac cgagtgcagc ggcccggagc cgcaggaacc ccagccggag
    21181 gaacccgaag ccccggagga agaacccaag ggcggcgcgg gcggcctgat cgtgttcctg
    21241 ctggtggcgc tggcgggcgg cggtgcggcc ctttactatt tcaagttcaa aaagcccaag
    21301 gctgaaacca ccggcagcga cgatttgggc gagtatgatt ttggggagga cgaggatttg
    21361 gaggacgagg aacccgaaac cgagtttgac ccggaagctg attttgtgag tgaacaggag
    21421 gacgaggaat gacatataaa cttgtaatcg cggaaaaacc cagcgtggcc cgcagcattg
    21481 cgggcgtgat cggggccacg gagaaacacg acggctattt ggaggggaac ggctatctgg
    21541 tgagctggtg catcggacat ctggtgtcct ttgccgacgc cggacgctat gacgagcgtt
    21601 tcaaaaaatg gcgctatgag gatttgccca ttctcccgga aacatggcag tacatcgtcc
    21661 ccgacgacaa gaaacagcag tttgacgttc tctgttcgct gatgcagcgc cccgacgtga
    21721 ccggccttgt gtgcgccacc gacgccgggc gcgaggggga attgattttc cgtttcgtct
    21781 accagatggc gggatgccaa aagccctttg agcgcctttg gatttcctct atggaggaca
    21841 gtgctattaa ggacggcttt gcccacctga aaccgggcgg cgactatgat ccgctgtatc
    21901 aatcggccct gtgccgggcc aaggccgact ggctgatcgg cattaacgcc acccggcttt
    21961 tttccgtcct gtaccacaag aacctgactg tgggccgcgt ccagacgccg accttaaaaa
    22021 tgctggtgga cagggacgcc aagattaccg atttcaagaa agagaaatat cacatcgtcc
    22081 atatcacggc gggcggcgcg gacgcggtaa gcagccgctt ttccgacgct gccgaggcaa
    22141 acaccgtcaa ggcggcttgt gcaaaagcgc aggccgtttg cgtttccgtg acgcgggaga
    22201 aaaagacgga acagccgccc aagctctatg accttaccac cttgcagcgg gaggctaacc
    22261 ggcttttcgg ctataccgcc aagcagaccc ttgactatgc ccaggcactg tatctaaaga
    22321 agctgctgac ctatccgaga acggatagtc aatatcttac cgacgatatg ctgcccgccg
    22381 tggaaagcct tgtgtccggc ctgtgggagc tggtgccgtt tgccaagggc ctgaaccttt
    22441 ccccgcagtt tgaccgcatc ctgaacagca aaaaggtgtc cgaccaccac gccattatcc
    22501 ccaccgctga atttgtgaag cagggctttg gcactctggc cgagagtgaa aaaaagctgc
    22561 tgtccctgat ctgctgcaag ctcttatgtg ccgtggccgg gccgcatatc tatgaggccg
    22621 tcaccgccac atttacctgt gccgggcagg agtttaccgc caagggcaag accgtccttt
    22681 cccccggctg gaaagagatt gaacgccggt tccgttcttc cttaaagacc gacgccgacg
    22741 aggacgccga ggcggtgcgg gagctgccgg agctttgcga gggccagacc tttgcagacg
    22801 tggccgcctc tgtcaccgag catttcacca cgccgcccaa gccctacacc gaagatacgc
    22861 ttttgtccgc tatggaacgg gccggggccg aggatatgcc ggaggatgcc gagcgcaagg
    22921 gattaggcac accggccacc cgcgccgcca ttctggaaaa gctggtgcag atgggctttg
    22981 tccagagaaa aggcaagcag ctcattccca ccaaggacgg catcaatctg gctgtggtgc
    23041 tgccggaggc gcttacttct ccccagctta ctgccgaatg ggaaaaccgg ctgaccgaga
    23101 tcgccaaggg gcaggccgat cctgacgaat ttatggacgg catcgagacg caggcgcggg
    23161 agctggtgca gacctattcc tgtatcagtg aggaaaagca gaaattgttt caggccgagc
    23221 gtgttcctat cgggacgtgt ccccgctgtg gtgaggctgt ctatgagggc aagaaaaact
    23281 actactgcgg aaaccgggcc tgccagttcg tcatgtggaa gaatgaccga ttttttgagg
    23341 aacgcaagaa agcgttcacc ccgaagatcg ccgctgcgct gctcaaaacc ggcaaagtca
    23401 aggtaaaggg cctctattcc gtaaagaccg gcaaaaccta cgacgggacg gtgcttttgg
    23461 ctgataccgg gggcaagtac gtcaactacc gcattgaaca caggagctaa aggacagtaa
    23521 cacagcaagc atagggagtg tggctacact cccggtaaaa tcccccggcc gcgtccctgc
    23581 cgggccgggg atttttgctt gttggggaag tccccaaccc cctatttttt gaaacagaac
    23641 agagaaagga gtgaccgcgt gaacgaagta ttttctatcc aagtccataa ccggcagttt
    23701 ttggaacagg gcaaacaagg cgtctggctg gatttgccca ccacaaccga aacgctgcaa
    23761 gccgccctgc gggatgttgg cattactgcc gacaatccgc aggatttttt tatcaacggg
    23821 tattcctgcc cggaggacag acaccttgcc attccctatg acatggtgct ggccgccgac
    23881 gtggacgagc tgaatttcct tgccgcccgg ctgggccagc ttgacgccgc cgagatcgca
    23941 gagctgaacg ccgccctgca aaatccccgt ggcggctttg agaatatcgg ccagatcatc
    24001 gactacaccg aaaatgtgga ttactatgtg catcttcccg acgttagggg ggatggacag
    24061 ttgggcgact actacctgaa ccgttccggc atggtggata tgccggagga atggaaagcc
    24121 gggattgata cggcgcagtt tggacgccac attgcccagc aggaacaagg cgtttttacc
    24181 cagtacggct atctggtgag aagcggcgac gaatggcagc gcgtccatga gggccagccg
    24241 gtgccggagg aataccgggt gatgcactac ccccagccgg aagctgaccg ggaggaaccg
    24301 caggcccagc agccggaggc atccgaaagc cccgacaaag cgccgccccc ggttatcccg
    24361 atcatgctgg acggcatgaa cagcaaagaa cgcatgaaag tgattactga ccgtctggaa
    24421 accggcgtac aggagctttt tgaaagtagc cgctatatgg cttacttatc cgctatgtcc
    24481 aagatgcacg attacagtct gaacaatacg ctgctgatcg ccatgcaggg cggttcgatt
    24541 gtggcgggct ataacaggtg gcaggatgaa tttgaccgcc atgtgaagcg gggcgagaaa
    24601 ggcattaaga tactggcccc ggccccgttc aaggttaagc agcagatgga gaaaaccgac
    24661 ccggccaccc agcagcccgt gatcggcgcg gacggcaagc ccgtgaccga agaacaggaa
    24721 gtgacggttc cggcctttaa ggtggtgaca gtctttgaca tcagccagac cgagggcaag
    24781 gattttccca tgccctatat cagtgaactt accggcgacg tgcagcagta tcaggatttc
    24841 tttgccgcgc tggaacggac atcaccctat cccataagca ttgagccgat ttcagacggg
    24901 gccaagggcc gctgcttcta cgcaaaacag cgcattgcca tcaacgaggg catgagcgaa
    24961 ttgcagaccc taaagaccgc catccatgag attgcccatg ccaagctcca cgccattgac
    25021 cgggaggccc ccgccgagga acaggcggcc cggccagacc gcagcacccg cgaggtacag
    25081 gccgaaagcg tggcctatac cgtctgccag tattacgggc tggacacgtc ggactattct
    25141 tttggctatg tggccgattg gagcagcggc agggagttat ccgagctgaa agcctccctt
    25201 gaaaccatcc gggccacggc cagcgaattg ataaccgaga tcgacggcca ttttgcccag
    25261 cttcagcagg agcgcgaggc ccggcaggaa caggaggccg tcacggttga cgttaccccg
    25321 gaggcagcgg ccccggcgca ggccgaggaa gaacaggccc cggcccttga tccggcagcc
    25381 gagccgcttg tgactgtgct atggagcgaa agcccccagc taaaggacgg ccagcaaatg
    25441 ccgttgcatg aggccgacgc gctgtttgcc cgtctggatg cagcatatcc ggcagaggca
    25501 ggctatgaca aaaccgcgtt ccgcattgac tgtatctttc agggcgagcc ggacagctac
    25561 gaggggcggc aggattttgg agatagggac ggttccctga tcgaacatat ccagagctac
    25621 caccaatttt acgcgcagga cgaaaactgg aaaaagcatg taatcgctac aaccggcata
    25681 gagggctggg agcaggacaa ggcccatcgg gatatgctgt taggcgagtt tgtcccctat
    25741 atgaagctgc attgcagcct tgcccaaatg gaacagacgg cgcagggccg cttacagtcc
    25801 ggggaaaccc tgacgccgga ggaaacggcc tatttggggg cgcttctctc ttatgtgcag
    25861 gagtgccgcc ccatgctcaa tcagggccag tatcagttgc cggagccgcc gaaactggct
    25921 gacttcgacc agagcttgca ggactacaaa gcacaggtac aggccgagat cgcgcaggag
    25981 gccgccgcag ccggtatgac cgtggaggaa tacgcggcgg ctggctatga agcaccccaa
    26041 caggacacct tttccatcta ccagcttcga ggcggggagg aaacccgcga ctatcggttt
    26101 gagccatacg agcgtttaca ggccgcaggg ctggccgtgg acagtgccaa ctatgacctt
    26161 gtttatacgg caccgctgga taaaagcacg tctttggagg atatttaccg cacattcaac
    26221 atcgaccacc cggcagattt taggggacac agcctttccg tttccgacgt ggtggtgctg
    26281 caccagaatg gacaggacac cgcccactat gtagacagct tcggctatat ggaagtgccg
    26341 gagtttttgc gggaaaatcc cctgcgtacc gccgaactgt ctacggagca gaacgaaaac
    26401 atgattgacg gcattatcaa caatacgccg cccgaaccgc agccggagga acccgaacag
    26461 ctatcttttg aggatgtggc agttgcttcc ggccagacac aggaggaacc cccggaggct
    26521 gcgcccccgc aaacggctgt ccgctattat cccatcaatg agggagccgc ccgccgcgcc
    26581 aaggaagcca tgagcttttc tgactaccgg cccggcagcg ccacggcgga ataccggcac
    26641 tatgtcgatc aggccgtgga aattgcggag cgccagaaaa agcgtgtgga gccagagtat
    26701 cacgaaaaga tagacagcct ccttgacacc tacgcccgca agctggccga gaacatgaat
    26761 aagggctatg agattaccgc ccgcgtcccc tctgtcctga ttgccgggcc gtccaatttc
    26821 ccggtacggg cgaaagaaaa gcagaacgcc gccagtgacc gcaacatgga ggaatttcag
    26881 tacattcagg gcttgcttga caaaatccgc agcaccggca tgggtggtat cagcgccgac
    26941 gatccgcagg ccgtttccaa gctggaaaag aaactggaaa agctggaagc gtcgcaggag
    27001 ttgatgaagg ccgtcaatgc ctactaccgc aagcatggga cgctggacgg atgcccccat
    27061 ctgatcgaaa gaggcattga aaacctgaaa gcggatatgg cgtcgggctg gcactatgag
    27121 aaaaaaccgt tccagtcctg gcagctctcc aacaataacg ccgagatacg ccggttaaag
    27181 ggccgcattg aggaattgac ccgccaaaag gaggcggcct atgtgggatg ggagtttgac
    27241 ggcggcactg tggagatcaa ccgggtggcc aaccgcctgc aaatcttctt tgaggggaag
    27301 ccggatgctg ctgtacgcga cgagctgaaa agcaacggtt tccgctggtc gcccaaggcc
    27361 ggggcatggc agcggcagtt aaacgacacc acaatccgcg tcgctgaccg tatcaagtgc
    27421 atccagcccc tttccggcga aaagcccagc gccttacagc ttgcggcccg cagggagaaa
    27481 gaaaagccct ctatccgggc gcagctcaac gcggccaaga ccgaacaggc caagcagcca
    27541 ccggcgcggg aaaaatcaaa gggattggag gtagagtaaa tgcagaagtt tgaaaatgtg
    27601 gacgttctgg ccgcgctgga acagatcatg cggcagaaca ccgcgtttta tcaaagtgac
    27661 tttggcattg acaaagacat tatcaggcgg gatgccgcca gcgatcaggc cgcagacaag
    27721 accctgctct ggatgtcccg gccctccggg acgtactgtt tccgggaacg tgatgttttc
    27781 ctgaaagaca cccgacagta caacacatgg aaattttacg gggagcagac ccgtgacaag
    27841 atactggcct acgcggtgga gctgaccggc acacagggcg gcactatccg gggcaatctc
    27901 tatgagctgg actatccgca gcatttccgg cacgtcattg aaaccgccca gccggtgagt
    27961 gtgaaccggc tcttttatga gcatggcacc cgtgacatcc cggagagcca gcgttttgac
    28021 ggaagccctg accgggtatt gggaaacttt ttgtactatg aggcccagcc ccatgacccg
    28081 gctgtgctgc aggaggcgct acggacggag cagcacagcc gggggcgggc cgtccccggc
    28141 gatttcaaag tccacgtcac cgctttgcat gacagccgga ttgaaaccga ggcccggcgt
    28201 attgtggaca gcctgaaaga tctgtccgcg cctaacagcc ccaataaaac gcatttcatg
    28261 gtggagatct ccccgcactt tgcggcgatt gcttccacca aggataatga ccggctcttt
    28321 gccatgctgc cctataaatc cctgtgtttt accgggatga aaggcaggca tggcgtgtat
    28381 gccgtgatta accgggacga gcgccgggat gtgagtattc gcaggccccg gccctctatc
    28441 cgcaagcagc tatccgacag caaacaggcc gccagcccaa aaaaggcggc ggccagaacc
    28501 aaaaagaatg aattggaggt ataaggaatg atgcacttta ccgtggagga agaaaacctg
    28561 atctgtatgt accacaatgc agaccggcgc agaacgataa acaggctcac cgccgtcatg
    28621 ccggttatgg atggggatat gcgggcgcag gcccagcaga ccctttccaa gctggaacgc
    28681 atgacggatg ccgactatga cggccagaag ttccacttca cagacgaata acgaacagca
    28741 gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc gtcttttctt tttgcctatt
    28801 cctacgcatt tagaacccgg tattgtcggg gataggatga agtattcgct tgcccctgca
    28861 aacatgcggg agaagccttg cagcgggcgg ttttcaatcc cttaaatgcg taggttgcag
    28921 ctataattag acccctgaaa cagatatagc cgcgcagcgg gtgtatctgt ttcaggggtg
    28981 tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc ctcttgccgt ctatctctgt
    29041 tccagcggga agctcggcaa tgcttccagc agtttcaggg aaatgcgctg ccgtatatct
    29101 tcgtcaatga ctttcttctt tttgccgttt gcctgtctga tctccaccgt ggaaagctcg
    29161 ttgatgtagt cagcataatg ggccaacact ttttccgtgg cccacctctc accggcaacg
    29221 gcggctttga tcgtttcata gtccagcagc ttttcttgtc cgtccatcct gatacccccg
    29281 tcagactgat tttttctatg ataccatatt cggaagcatg gaaccagaga aaacggcgct
    29341 tttctgctgg taaattctac gcatttagaa cagcacttcg gcggggatag gataaaacac
    29401 ccacataccc cgctgaccat gccggaaaag ccttgaaata taagggcttt tacagctcta
    29461 agtgcgtagg tttccagtat cttttccgct ggcgctcggc ctctttgcgg cggcgcatcc
    29521 gtttggagca ggccgcgcag tatttggccc ggttagactt cggcgtgaac gtggccccgc
    29581 actcacagca gcgtttcact tcgtcccggt ctttggtgat ctcggcatac agggccgcat
    29641 ccagcgggag gacagcggcc ttaaaccagc ggcacagaag cgaataggaa atgctctgta
    29701 cgcaaataca ttcttcgccg tcgtccagca acaggcagtt gccgtcacaa tagttgcagc
    29761 actcatgcgt cagcttccgg gccttgcgat attgagggta attcattcgt ggaatactca
    29821 tagctcttgc gtccattcct ttcccggcgt catacgcaaa atgccgtcca cattctgctt
    29881 gatgatgccg tattcctgca tctgcttttt cgcttcgccg tacttctcat acagccgttc
    29941 cttttcctcg gccaatcgct tgtactcggc tttcagggcg tacagatcgg ggagcttttt
    30001 cacaccctgt ccctttaggg cctttgccgc agcttcatac aaaatcaacg tgctttcatg
    30061 ctcacgccgg aattttgcct tatccgctgc attgcggtat tccatagcca cgggccagag
    30121 ctgcttgtag gtagaaatat tctttatcag caccgccata tccgcaagcc ggtgttccat
    30181 agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg atctcggcca ctttgctttc
    30241 caaatccggg taggtgtcta tcccatgttc agtcaggaaa ttcaaagtct ttgcggcctg
    30301 cttcaaattg tggagcttcg cccacctctc atagcctgcc gactgctggg ccttaatgtt
    30361 gttttccaga tcaatccgca aagaaatccc cttgcgctct ttgggcgctc tggtgataac
    30421 gcggcctttg atacgctctt tgatggcttc ctcggtgtag gcttccccca atgtcttgca
    30481 gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata aactttcccg gcttgatctc
    30541 atagccctgc gcctgcatga gccgcaggaa atcatcaaaa tcttttgcct gcgggatagc
    30601 ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc cctttccgct gggcgtccca
    30661 ctcggcatag ctcttgccct tactctggcc gggcttcacc acggacaatc cgttttcctt
    30721 gcacagcctg tcgctggtgc gccggatata ggcatagctc tgtttgttgg aaatgtactt
    30781 gcgctgattt accatatcca cggcacagaa aataatgtga ttgtggacgt ggcccttatc
    30841 aatatgggta gtcagcacat agggatattt cccttgcagc acttcctcgg caagctgctt
    30901 cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg gcaaaggatt gtatcaggtg
    30961 gtggcccaga ttattgccct tttggtatgc ctgcgccagc agcatttcaa attcaatgtc
    31021 cgctgtttca taagagcagg caaaggaggc caccagcagc ttgccgtcag ttttgtccgg
    31081 gttctcgatg tagtcaaggg cttttttcag cgttgacttg ataggatgta tctttgtaac
    31141 tgccatatct gcgccagcgc cccctttata tcctcgatgt cctgtgcgta caccggcccc
    31201 ataccgttca gcctctttgc aatctgattg acgttgacgc cgatcttctg tatctcggcg
    31261 gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat agccgtcaat gagcatcttc
    31321 cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca ttttctgttc aatcatagcc
    31381 cgttcctccg gcgtcacagg gacgcgcagc acgatttttc gctttctgtt tgccatgctt
    31441 caccgtcctt tccattcagg ggtttgggga tttccccaac aagcaaaacg gacgcggccc
    31501 ggcagggacg gggcaggccg tttttccggg agtggctaca ctccctatgc ttgctacgtt
    31561 actgtttctc cactaacaag acgtttccaa agccccaaac tacccgcaaa atagccctct
    31621 ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc agctcccgct aaaagtaaaa
    31681 cagaggatgc cagttacaca tcctccgttt cattcttctt cggcttttca attagtatgg
    31741 cgctgtcaat cgcgccctcg atgatcggca ggtattcttc ggggcagagt ttcagcttgt
    31801 ggctgaccct ttgccgcagt tcgctttcct cccgcatgat ctccgggtta aagtagcgtt
    31861 ccacaggaag cccgcaggct ttaataagct gtatgacaac cggcagactg ggaatggccc
    31921 gtcgttttcg atgttggcaa gatagcgcca gtcaatattt attttctctg ccattgcttt
    31981 gcgcgaaacg cgctttgctt gccgggccgc tttcacatcc gcgccgaaag tttcaaagcc
    32041 gggacaatct tcaatattcg ccatatcgca cctcccactt acattgtata tttcatactc
    32101 tgcccgtgga atgttggtat atgcaccttt cttaaagttt atatttcaca ttttgtttct
    32161 tttattcttc gagcaaaaga attataatgt accctgtgga ggtgtactat ggactacatg
    32221 acgttgaaag aggcaggcga gaaatggggt gtgcccccac gatggataaa ctactattgt
    32281 gctgcgggac gcatccccgg cgctgtaaag atggcgacga tctggctcat tcccaaaact
    32341 gcggaaaagc cgattgacag gcgtacaaag aaaggaaagg cggttactga atagtataat
    32401 gaaaccggcg cattttggat atggttttga agagagagaa agcatggtat tttgtcacgg
    32461 aaaaactata agatgataga tggcaggctg ctccagaccg ataaaaagta ttctcatctg
    32521 aaacagaaag aaaagattta cacatggatg tatgaagaag cgaaacttta tcatgacaga
    32581 aatggaaaat gcccgaaaca aagacgagga agctgctgct gtatcagccg tatatggtag
    32641 aattgaaaag gccggcatct ggattcttta tggtgaagtc tccagacatt ggataactgc
    32701 tggtctaatg cgcctttgag tatggggtga ttgattttaa taccggggag gtacggcttt
    32761 ttgaggaaaa tattctggga tttacatttg gcgattatta catttcctgt aactgcaagg
    32821 acacggatga tgaagatatg aggaatatag acttgctgat tttctactgt cttgaacggt
    32881 attaggactg aatctgctgc gcggagtgcg gcaagagcga ccggagggag tacacacagg
    32941 ccctgcctgt gtgaaaagcc ataccgcaag gtgcattcgc aaaaaagagg ccgcgcattt
    33001 tgctgaggct tggggtgtcc ccaacaagca aaacgagaac gccgcaggcg ggcaagtggt
    33061 ggatcagaat atccgtaaag gatattctga tccattgctt gccagtatta tcgggatgtt
    33121 ttgacaaccg ctgtaatcga aacatcccaa acattaagaa ggtgttaatt attattatct
    33181 tgatttaatt cgtttaaacc aataatatag atacatacac catgaaaaga agcgagtatc
    33241 ccaatgacgg atgattaaaa tccgaaaaga aatcgctttg aaactacagg gaaactacag
    33301 actgttatgt tatcttctta aaatggaggg atttttatgt cgatacgaat tctacttgtc
    33361 gaggatgatg atcatatctg taatacagta agggcgtttc tggctgaggc aggatatcag
    33421 gtggatgcct gcacagatgg aaatgaggca tacaccaagt tttacgaaaa cacttatcaa
    33481 ctggttattc ttgatattat gctgcccggt atgaacgggc atgaactttt gcgtgaattt
    33541 cgtgcgaaaa atgatactcc cattctgatg atgacagccc tgtcggatga cgaaaaccaa
    33601 atccgggcgt ttgatgcaga ggcagacgac tatgtaacaa agccattcaa gatgcagatt
    33661 ttactaaagc gggtggaagc cctgctgcgg cgcagcggtg cgctggcaaa ggaaatccgt
    33721 gtcggcaggc tgacacttct gccggaggat ttcacggtac tttgtgacgg tacagagctg
    33781 cccctgacac gaaaagaatt tgaaatcctt ttgctgctgg tgcagaacaa aggcagaacc
    33841 ttaacgcatg aaatcatttt gtcacgtata tggggatatg actttgaggg tgatggcagc
    33901 acagtccaca ctcatatcaa aaatttgcgg gccaagctgc cggaaaatat catcaaaacc
    33961 attcgcggtg taggataccg attggaggaa tcataataat ggaaagaaaa gggattttca
    34021 ttaaggtttt ttcctatacg atcattgtcc tgctactgct tgtcggtgta acagcaacac
    34081 tgtttgcaca gcaatttgtg tcttatttca gagtgatgga attacagcaa acagtaaaat
    34141 cctatcagcc attggtggaa ctgattcaga atagcgatag gcttgatatt caagaggtgg
    34201 cagggctgtt tcactacaat aaccaatcct ttgagtttta tattgaagat aaagagggaa
    34261 gcgtactcta tgccacacca aatgccaata catcaaatag ttttagaccc aactttcttt
    34321 atgtggtaca tagagatgat aatatttcga ttgttgctca aagcaaggca ggtgtgggat
    34381 tgctttatca agggctgaca attcggggaa ttgttatgat tgcgataatg gttgtattca
    34441 gccttttatg cgcgtatatc tttgcgcggc aaatgacaac gccgatcaaa gccttagcgg
    34501 acagtgcgaa taaaatggca aacctgaaag atgtaccgcc gccgctggag cgaaaggatg
    34561 agcttggcgc actggctcat gatatgcatt ccatgtatgt caggctgaaa gaaaccatcg
    34621 caaggctgga ggatgaaatc gcaagggaac atgagttgga ggaaacacag cgatatttct
    34681 ttgcggcagc ttctcatgag ctaaaaacgc ccatcgcggc tacaagcgtt ctgttggagg
    34741 gaatgcttga aaatatcggt gactacaaag atcattctaa gtatctgcgc gaatgcatca
    34801 aaatgatgga taggcagggc aaaatcattt ccgaaatact ggagcttgtc agcctgaatg
    34861 atgggagaat cgtacccata gctgaaccgt tggacatagg gcgcacggtt gccgagttgc
    34921 tgcccgattt tcaaaccttg gcagaggcaa acaaccagcg gttcgtcaca gatattccag
    34981 ccgggcaaat tgtcctgtcc gatccgaggc tgctccaaaa ggcactatcc aatgtcatat
    35041 tgaatgcagt tcagaacacg ccgcagggag gcgaggtacg gatatggagt gagcctggtg
    35101 ctgaaaaatg ccgccttttt gttttgaaca tgggcgttca cattgatgat actgcgcttc
    35161 caaggctgtt caccccattc tatcgcattg atcaggcgcg aagcagaaaa agtgggcgaa
    35221 gcggtttagg acttgccatc gtacaaaaaa cgctggatgc catgagcctc cagtatgcgc
    35281 tggaaaacac ctcggatggc gttttgttct ggctggattt accgctcaca tcaacattgt
    35341 aaatatttaa aacttaaatg attt
//
LOCUS       DACCXW010000012        34771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCXW010000012 DACCXW010000000
VERSION     DACCXW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.012"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(227..1114)
                     /locus_tag="IVY71_000496"
     CDS             complement(227..1114)
                     /locus_tag="IVY71_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7984569.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="IVY71_000497"
     CDS             complement(1111..1980)
                     /locus_tag="IVY71_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7984570.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="IVY71_000498"
     CDS             complement(2117..3403)
                     /locus_tag="IVY71_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7984571.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="IVY71_000499"
     CDS             3676..4254
                     /locus_tag="IVY71_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7984572.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="IVY71_000500"
     CDS             4254..5183
                     /locus_tag="IVY71_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7984573.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="IVY71_000501"
     CDS             5180..6916
                     /locus_tag="IVY71_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7984574.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="IVY71_000502"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="IVY71_000502"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7984575.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="IVY71_000503"
     CDS             complement(7812..8321)
                     /locus_tag="IVY71_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7984576.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="IVY71_000504"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="IVY71_000504"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7984577.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="IVY71_000505"
     CDS             complement(9622..10713)
                     /locus_tag="IVY71_000505"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7984578.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="IVY71_000506"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="IVY71_000506"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7984579.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="IVY71_000507"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="IVY71_000507"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7984580.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="IVY71_000508"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="IVY71_000508"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7984581.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="IVY71_000509"
     CDS             complement(14676..15650)
                     /locus_tag="IVY71_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7984582.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="IVY71_000510"
     CDS             complement(15631..17514)
                     /locus_tag="IVY71_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7984583.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="IVY71_000511"
     CDS             complement(17532..19757)
                     /locus_tag="IVY71_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7984584.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="IVY71_000512"
     CDS             19872..20906
                     /locus_tag="IVY71_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7984585.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="IVY71_000513"
     CDS             complement(20973..21986)
                     /locus_tag="IVY71_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7984586.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="IVY71_000514"
     CDS             complement(22064..22252)
                     /locus_tag="IVY71_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7984587.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22692..22820)
                     /locus_tag="IVY71_000515"
     CDS             complement(22692..22820)
                     /locus_tag="IVY71_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7984588.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22823..24196)
                     /gene="rlmD"
                     /locus_tag="IVY71_000516"
     CDS             complement(22823..24196)
                     /gene="rlmD"
                     /locus_tag="IVY71_000516"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7984589.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24307..25362)
                     /locus_tag="IVY71_000517"
     CDS             complement(24307..25362)
                     /locus_tag="IVY71_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7984590.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25372..26802)
                     /gene="gatB"
                     /locus_tag="IVY71_000518"
     CDS             complement(25372..26802)
                     /gene="gatB"
                     /locus_tag="IVY71_000518"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7984591.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26799..28271)
                     /gene="gatA"
                     /locus_tag="IVY71_000519"
     CDS             complement(26799..28271)
                     /gene="gatA"
                     /locus_tag="IVY71_000519"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7984592.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28271..28576)
                     /gene="gatC"
                     /locus_tag="IVY71_000520"
     CDS             complement(28271..28576)
                     /gene="gatC"
                     /locus_tag="IVY71_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7984593.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28682..30718)
                     /gene="ligA"
                     /locus_tag="IVY71_000521"
     CDS             complement(28682..30718)
                     /gene="ligA"
                     /locus_tag="IVY71_000521"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7984594.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30735..32969)
                     /gene="pcrA"
                     /locus_tag="IVY71_000522"
     CDS             complement(30735..32969)
                     /gene="pcrA"
                     /locus_tag="IVY71_000522"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7984595.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33124..34683
                     /locus_tag="IVY71_000523"
     CDS             33124..34683
                     /locus_tag="IVY71_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7984596.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9518 a         7494 c         6336 g        11423 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aactaaaaag actaagaaac aataacaaaa gttaacactt cttagtcttt
    22681 ctttagcacg tctatttatt aaagatttta gggccttttc tcatgttttt acgatcattc
    22741 ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt tctttttgat caattctttc
    22801 attttttctt tttggtccat ttttattctc ctttaacgaa tgaagcgact aattcgatat
    22861 gatgggtttg cgggaaaaga tctacaggct gaactttctt cacggcataa cctgcttctg
    22921 caaataactt agcatctctt gcaaaagttg ccggattaca tgagatatag acgattcgtt
    22981 ctggtgacat ttcgattgct gattcaataa aatgatgatc taatcctttt cgtggagggt
    23041 caactacgat cacagatggt ttgatccctt tttcttgcca ttttttcata actttttctg
    23101 cttttccgac ttcataatga acattttcga ttttattatt taaagcattg aactgtgcat
    23161 cttcaattgc ttctggaata acttccatcc catacacctt atccactctg tctgctaaag
    23221 acaagccaat cgtacctatt cctgaataag cgtcgatcac aatgtctttt ttatctaact
    23281 cggcaaactc gatggctgtt tggtacagat actctgcttg ttgagtattt acttgataaa
    23341 atgactttgc tgagattcga taggtttttc ctaatagctg atcttcgata tagtctctgc
    23401 catataaaac tttttctttg tctcctaaaa tcacatttgt tttttcttca ttgatatttt
    23461 ggatgaccga aaccacttct ggcaattctt catgaatagc ttcagcaatt tccttggatt
    23521 taaagaattt ttctttacga gtaactaaaa cgaccatcat ttcatgggaa taatgaccac
    23581 gacggataat gatgtgacga agctgtcctt catttttttc ttcattatat cctcgtactt
    23641 ggaaccgttg caagatatct ctgactactt tgattgcttg atcgatgaca ggatcttgga
    23701 tataaaaatc ttcgataggg actagatcat ggctattctt tttgtaaaaa ccggtggcta
    23761 actgtccatc gatacggcga acagggatct gtgctttatt acggtagccg aatggttctg
    23821 ccattccgac cgttggaagt acaggaactt ctggcatctt agcaatcttc gccattacat
    23881 gctctacttg tttttgtttg aagacaagct gttgttcata cttcaaatgg gctaaaggtg
    23941 cgatacctat gcgaagtaat gcattgtttt ctacaggctg acgatcaggt gattgtgtaa
    24001 taaatgtttc gacttttcca tatccaaact tattgcctgc ttttactact agtatttcta
    24061 cttcttctcc agggagtgca ttttcgataa ataatggata accatcgatt tttgctaccc
    24121 ccattccttc ataactcaag tcgacgattt cgactgtata tcgttcgttt ttctttacta
    24181 tttgctgttc tgtcatattt tgctccttta agcctatttt tcaatcctac tgaactctac
    24241 tataaatcag ctaattacac aactaaaacc ggcactgacc ttactacgtt cagcaccggc
    24301 ttttgattaa ttcttttgat taggtgtttc gtctgctatt tttccgtcac cgtcgatatc
    24361 ttcatccgtt aaacgttcga cttcttttac gaattctttg ctgacaacct ctaattcttc
    24421 ttcatctgta cctgtgattg cttcatcagg aatctgatcg gtattggcaa agaactcaat
    24481 gtgttgatga agattttgga aatgcatcgg tgcttccccg ccgtattcac catctaagtt
    24541 gatcattact ggtcgattga tatcatcttc tgtttcaacg aacaaactgc ttgttttggt
    24601 atagattatt cttggatcat cgacatgtcg accgccattc agcatgagtg ctgcaatatg
    24661 caagatctca aagatatttg ctgtcttcac aatgattaaa gaaaacttcc catcatccaa
    24721 tttggcatct ggcgcgatct gttcgaatcc tccaacagag ttcgtcagcc ctaggaaaaa
    24781 catcgaagca tttccgatat attccccttc atcatacttc aagcgcattt tgattggctt
    24841 tactcgaggg agcatctctg ctcctttcgc aagataagca agataaccaa aaatactctt
    24901 tagttctgat ggaacttcat aagtcaattc tgtcaaatag ccacctgcag cgatattgat
    24961 gaagtaactg ttatcagaaa ggccgatgtc catttttacg gtttggtgtt tttttatcac
    25021 ttctgctgct gctttaatat tatctctagg aatttttaat gctcgagcat agtcgttcgt
    25081 tgtaccagct gggatgatgg ccattttagg acggtgttca agcggagcga ttccattaac
    25141 cacttcgttg attgtgccat cgccaccagc tgccactact agatcaaaac ctagttctgc
    25201 gactctacat gcttcattct tggctgaatc tggttctggt gtagttgcat aggcgctggc
    25261 ttcaaatcct gcttcttcta atgcctgtag gatatccgcc aaattttttt tcaataattc
    25321 ttttcccgag gtggggttat aaattacgcg tgcttttttc attttcagcc tctatcgttt
    25381 cgctaattca tctttaagta gcttattcac gacaccagga tttgcttgtc ctttagttgc
    25441 tttcatgatt tgaccgacta ggaagccaac cgcacgatct tttccatttt tgaaatcatc
    25501 gacagattgt tggttgttat ccaaaatttc attaatgatt ggcagtagtt tgttcggatc
    25561 agataattgc acccaaccat ttttttcaac tacttcttgg gcttctccgc catttgtgat
    25621 caattcacgg aagacttttt tggcgatttt tgagctgatc gttccatcgg cgatcaaacg
    25681 aatcattcct gctaagttct caggtgtcaa ttttgtctca gctaattcca gtttctcact
    25741 gttcaagtaa gcagaaactt cacccatcaa ccagttagat gcttgcttgg catctgcacc
    25801 attttccaaa acagcttcaa aaaaatcaga catttctttt gtaagtgtca ataccattgc
    25861 gtcatactct ggaagtccaa actcacggat atagcgttcg cgacgagaag ctggcatctc
    25921 aggcaaacta gcttttactt tttgaatcca ttcgtcatcg atcacgaatt tcgggatatc
    25981 aggctctggg aagtaacgat aatcactaga cccttctttg acacgcatca aaactgtttt
    26041 attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt tctcctcctg aaagaagaac
    26101 ttttgcttga cgtttttctt caaaagctaa accttttttc acgaaagata aagagttcaa
    26161 gtttttcaat tctgctttcg taccgaactc ttcttgtcca taagggcgaa gagaaatatt
    26221 ggcatcacag cgcatagagc cttcttccat tttcacatcg ctgacttctg taaattggat
    26281 aattgatcgc aatgcttcta gataagcgta tgcttcttct ggtgaacgca tatcagcttc
    26341 agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa tcaacgtaag aataaccgcc
    26401 aataccatgc atgttttttc cagcatcttc ttctaaatgg acacgttcga tccggatttt
    26461 tttcttttta ccttctactt cgatttcgat ccatccgtca tgaccgattg gttgatcaaa
    26521 ttgggaaatt tgatatgctt ttggattatc tgggtaaaag taatttttcc ggtcaaaatg
    26581 agtatcttta gagatttcac agtttaatgc caaagctgct ttcatcccga attccagcgc
    26641 agctttattc attactggca gcacgcctgg ataaccccag tcgatcacgt ttgtattgct
    26701 gtttggttct gcaccaaaat gagctggcgc tggagagaaa atttttgagt tggtttttaa
    26761 ctcgacgtgg acttctagtc caatgactgt ttcaaaattc attcgtcttt ccctcctaaa
    26821 atagctggtt gttgtttatg gaattctgtt gcttgttcaa acgcatatgc cgcttgatac
    26881 atcgtttttt catcaaaata attaccgatg atctgcaatc caactggtaa tccttctgat
    26941 aaacctgccg gtacagacat acctggaagt ccagccaagt tgacagggat cgttaagatg
    27001 tcactcatgt acattgtgat tggatcgtta atgttctcac cgattccgaa tgcaactgtt
    27061 ggtgaagatg gcccgatgat caagtcataa tctgcaaaga ctttttcaaa atctcgtttg
    27121 atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt cataataacc cgcgcttaag
    27181 gagaatgtac ctaacatgat acggcgtttt acttcttcac cgaatccttc tgaacgtgag
    27241 ttgacatata catcatctaa tgatttcaca ttttcagagc gataaccgta gcggatacca
    27301 tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa tgatataata aacagccaca
    27361 ccgtattttg aatgaggcaa gctgacttct tccacaacag cacctaatga acggaatgtt
    27421 tctgctgctt tcaatacagc ttcttttacg ccaggggcca caccttcgcc taagtattct
    27481 ttaggtaaag caatcttcat tcctttgatg tcgcctgtca gtccctctgc aaaatcagga
    27541 actgaaacgc ctgaagaagt accgtctttt tcgtcataac cgctgatagc tgtcaatgct
    27601 aatgcattat ctttcaccgt acgggtcatt ggtccgattt ggtcaagaga tgaagcaaag
    27661 gcaatcaaac caaaacgtga gacacggcca taagtaggtt tcatccctac gatgccgtta
    27721 aaagctgctg gttggcggat acttccccca gtatcactac ctaaagaaac tggaatttgt
    27781 ccagctgcaa ctgctgctgc agaaccacca gaagagccac caggtacttt tgttgggtcc
    27841 caagcatttt ttgttttctt gaaataagaa gtttctgtac ttccacccat agcaaattca
    27901 tccatgttta gcttaccaac agggatcata tctgcttgat agattttatc catgaccgtt
    27961 gcatcataaa ttggttcaaa attgtaaagg atcttagatg cagcggttgt caagatatct
    28021 tttgttacga tattatcctt gataccaatt ggaataccag caaggacgtt gctttcatca
    28081 atcccttttt catctaaagc ttttgcctgt tccaatgctt tttcttcgtt caatgtaatg
    28141 aatgcctcga cctcaggctc tgtttctttg attcgttcaa atgttgtttt tgtcaaatct
    28201 tgtacagtga tttctttcga tacaagcata tgatgcagtt cttctagaga acgattgtgt
    28261 aattctgtca ttatgcgcca gcctccccat tatcgatgat tgcgggtact ttgataaagc
    28321 cgtcttctgt ttccggcaca tttttcaata attcatcgcg gttccatcca gcttgcgccc
    28381 gatcttgtcg catcacgttt actgtttcaa ccacattcga cgtaaatgga acaccttccg
    28441 tatctacttc ttccagcatt tcaaccatat cgatgatttt acctaattgg tcggtaaaac
    28501 ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt ggcaacgtgt ttcacttgtt
    28561 cttcactaat cgccatacta ccccttcttt ctaaacgagt taaaaacttt gctctctatt
    28621 ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat ttgtctgaaa aaactcattt
    28681 ttcattcttc tactgagcga cttttagcca atgcatctgc catttgatct tctgtccaaa
    28741 cttcgattcc taattcttgt gctttggtca gcttgctgcc agcatcttct cctgctacca
    28801 cgatatccgt ttttttcgat acgctaccag tcactttccc gccaagattt tcgatcgtct
    28861 cttttgcttc ttcacgagta aatcgtgtta gtttaccagt aaggactact gttttttctt
    28921 taaaaggaga ctcgacttct tgcaattggg catttcgaag ccctttgtat tcgaaattga
    28981 cgcctgcttg ttttaattca ttcatcaatt catggacttc ttcgttactg aaataagtga
    29041 ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc aataatcgac tcatagtcac
    29101 ttttactcaa tgtttctaga tcaccgaaat gttctgctaa aatttttgct gctttcgctc
    29161 cgacatgtcg gattcctaac ccgaaaatca accgctcgac cgaattatct ttgctgttgt
    29221 cgattgctgt caaaatatta tttgctgatt tttcttttat tttgtccaac gtcaataact
    29281 cttcttctgt caatttgtaa agatcagcaa catctgctac tagtttttta tcatacatct
    29341 gttctagtac gcgcggcccc aacccatcga tattcatcgc atttcttgaa acaaagtggt
    29401 tcaaaccttc tttcatttga gcagggcatt taggattgat gcaccgtaag gctacttctt
    29461 catccaaatg gacaagttca cttccacaaa ctggacaatg tgtaggcaat tggtattctt
    29521 cgctgtcttt aggtcgttta tctaacacga cttgcgaaac ttctgggatg atgtctcccg
    29581 ctttataaat cagaactgta tctttcaagc ggatatcctt caacttaata taatcaccgt
    29641 tatgcaggct ggcacggctt actgtcgttc ctgctactcg gactggtgtc atgatagctg
    29701 ttggcgttac tacccctgtt cgaccgactg tccattcgat gttttcgatt agcgtttcaa
    29761 cttcttctgg cggaaacttg taagctgctg cccatctcgg tgctttgata gtaaaaccaa
    29821 gttgatcttg tagcgagaat tcattcactt tgatgacgat tccatcgatt tcatagggca
    29881 aatccacacg tttgtcatga tattcttcaa tgtatgacca cacttcatca atcgaatgac
    29941 atagacgttt ttcccgattc gtgtggaatc caattttttc tagctcttct agcgcatcat
    30001 actgggtttt tgcttgcata ggaccgaaat cagctactgt gtataaaaaa gtgtccagat
    30061 ttcttttcgc tgtgatttta gaatctaact gacgcaaaga accagctgcg gcgttccgag
    30121 gattcgcaaa gatatctttt ccctctgctt ctctgtcttg gttcaattgg acaaatgacc
    30181 gttttggcat gaaacattcg cctcttactt cgatgttcat tggttctttt aatttgattg
    30241 ggaccgaccg gacagttttt aggttttcag tgatgttttc tcctacagtt ccatccccgc
    30301 gagtcgcacc tctcacgaaa tttccatctt cataacgcaa agaaacggaa agtccatcta
    30361 tttttagttc acagcaatag tcaactacac gtcccactgc tttttgtaca cgttgatcaa
    30421 aagctatcag ctcttctttg ctgaaaacgt cgttcaaact gtaaagcgga atatcatgag
    30481 tgactttttc aaaaccttct aatacttttc cgcctactcg ttgagtggga gaatcagacg
    30541 tgatcaaatc aggatattgt tcctcgattg ctactaattc tgcgtattct ttatcataaa
    30601 catagtcttc cactgttggt ttatcttcta cgtagtattc gcgagaccat tggttcagtc
    30661 ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat cgtcatttca tgttccatca
    30721 tttaccctcc actactaagc tttttcaatt ggcgcaaagg cagccaaaag acgttttact
    30781 ccttgacttg ggaaagccac atctagttct agatccttcg acgtcccgct tactcggacg
    30841 actgttccta gaccccattt tttgtgtttg actttatctc caggtttcca gctgttattt
    30901 tcccctccag tagcactttt agagggaaca accgctgtct ctggctgttt atagctatat
    30961 gctgtttttt tagcgaatac aggcgttttt ttaggcgtag gacgcatgcc ttcgatttcc
    31021 agtaactcct gatcgatttc ttctacaaaa cgagaaggtc ggttatattg cgtccgtcca
    31081 tataatgttc tggagaaagc atttgttaaa tacaacgctt cttctgctcg cgtgatcccc
    31141 acatatgcga gacggcgttc ttcctccagt tcgctttctt ccatcaatgc tcgagataaa
    31201 ggaaaaaccc cctcttctag accaatcagg aatacaactg gaaattctaa tcctttggct
    31261 gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt ctaaattgtc aatgtcggaa
    31321 acaagtgcca gatcatttaa gaagaccgtt agtttttctt ctggtgcatc agcgtcttct
    31381 tcattttgct gttcgaattg tttgtcgaat tcttgtgtga ccgttaaaaa ttcctccaag
    31441 ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt ttaagtcttc tagatatccg
    31501 ctacgatcca aaacctcttt cgtcaattct gtcactgtca agtatgggat catctgagtg
    31561 acttcttgga tcatctctcc aaatgcacct aattgctgtc ctgcttttcc accgatattt
    31621 gccagatcca cattttgcgc tgcttcaagc agtggccatt catgaagaga agcaaaactg
    31681 cgtagttttt cgatagatcc tggtccgatg cctcgtttgg gcgaattaac gatacgctca
    31741 aaactgattg aatcttgagg atttaccagc acattcaaat acgctaaaat atctttgatt
    31801 tctttacgat cgtagaactt gtgccctcca accattttat aaggaatatt tgctttcaac
    31861 agagtttctt ccattacacg agattgtgcg ttcgtccggt acaagatggc aaagtcgccg
    31921 taattccgat gattgcttcg aatctcttct tgcatccggt ctacgataaa acgggtttcg
    31981 tctcgttcat tatctgcacg gtagtaagtg atcttgtttc cttctttgtt ttctgtccac
    32041 aagttttttg gttttcgatt gctattgttt tcaatgactt ggtttgctgc gctaaggatg
    32101 tttttcgtgg agcgatagtt ttgttctagc aaaattactg cagcatctgg ataatctttt
    32161 tcaaaatcca agatgttctg catatctgca ccgcgccagc cgtaaatact ttgatctgca
    32221 tctcccacga cgcacaggtt cctaaatcta cctgccaata aattgactag cgtatactgt
    32281 gcgtgatttg tatcttggta ctcatctaca tgaatataat ggaatttgtt ttgataatac
    32341 gtgagtgaat caggatgttc ttcaaataat cgaatcgtat tcatgatcag gtcgtcaaag
    32401 tccatacatt gattatattg aagctctttt tggtaagcag cataacattt tgccgcaatc
    32461 tcttcgtaat aactgccttg catttttgca aaatcctgag gggtctgcag gctgtttttt
    32521 gcttgagaga ttgttcctaa tatacttcga ggatcatatt ttttcggatc aatgttcagt
    32581 tcttttagta tccgtttcat caaggtcagc tgttcagaag aatcgatgat tgtaaagttc
    32641 cggttatacc cgataaaatc aacatctctt cttaaaatac gcacacacat cgagtgaaaa
    32701 gtcgataccc agacatcttc gcctccagat gcgagaatcg cattgacacg ttctttcatt
    32761 tcttttgcag ccttattggt aaaagtaatc gctaggatgt tccaaggatt gacttctttt
    32821 tcctcaatca aataggcaat gcggtgtgtc agcactctcg tctttccact tcctgcacct
    32881 gccattagca gtaatggtcc gtcagtgtga agaacagctt ctttctgacg ggggttcatc
    32941 ccgtttaata attcagcttt ggatgacatt aacaacatcc tttcttttat caatcttgtt
    33001 aattatagca ttttcttggc tttcattaaa ctagctagtt caaaatcatt tgtttctttt
    33061 tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa ttaaaagaaa gcgaggcgtt
    33121 tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga ctcagatata ttttattcta
    33181 aaaggaatat ttgttggact gctgacagga atcgtcgtta gcctgtttcg attatctatc
    33241 gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga gtcgggaaca gccgatttac
    33301 ttaattggcc tagctatttg ctgcatcctt gcagcatttt tcgtgggtta tctggtcaaa
    33361 aatgaaccgg atatcaaggg aagcgggatt ccacaagtag agggacagct tcgcggggaa
    33421 ttatcaatga actggttctc tgttttatgg aaaaagttca ttggcggtgt actttcagtt
    33481 ggtgcgggcc tttttctagg tagagaagga ccttctatac agcttggcgc atccattgga
    33541 caaggagctg gacaattgtt tcggagtcca tcttcagaag aaaagatttt aatttctagc
    33601 ggtgcaagta gcggacttgc agcagcattc aatgctccta tcgcaggatt actctttgtt
    33661 ttagaagaaa tccatcactc cttttctcca cttgtttggc tgacatcatt tgcttcagca
    33721 atcactgcta acttcgtctc tctttacttt ttcggcttga gacctgtttt atatctaggt
    33781 gaccttcctt ctttgccgtt gaaatattat ggttccttag tgatcttagg agtacttcta
    33841 ggcatcttag gttttattta tcagaatgtc ttgctggctt tgccaaaatt ttacaaccga
    33901 ttgcctttac cagcttattt tcacgggttc gttcctttct tgctgatttt gccaattggt
    33961 tatttatggc ctagtactct aggcggaggc aatcaaatcg ttttagcttt tggacatgct
    34021 tccttgccaa tcattatgat tatcggtttg tttgttcttc gattcgtttt ttccatgatt
    34081 tcttacggtg ggggtcttcc aggggggatt ttccttccga tattgacatt gggggcttta
    34141 atcggtagct tttatggaaa cattatggtt gatttcggga tggatccaat ctacatcaag
    34201 gattttgtcg ttattgccat ggctggttat ttcacagcga ttggaaaagc accgctgaca
    34261 gcaattattt tagtgacaga aatggttggc tctcttaatc atctgatgcc tttaggattg
    34321 gctgctcttg ttgcttatat cgtcaatgac cttctcggcg gtaatccaat ttacgaatca
    34381 ttattggaac gtctacttgg aaatactcag accgcctcac taaaaggtgc aaggacaact
    34441 tttgattttc cagtaacggc agagagttct ttagacggat taatggtacg agacttcaat
    34501 tggcctaaaa atatgctgct aatctctata cgcagaggaa atcaggaaat cttgactcac
    34561 ggtgatacga tcatgaatgt aggagatatt ttggttatct tgactgatga agcaaacttg
    34621 ccaagaatca agcaggaaat cgaggctaaa tcttctatca caaatttgaa gcaatcattt
    34681 taacgaacat ggaaaaacta ttttacaaca actattcatc tacactactt gattctatat
    34741 gattctataa aaaaagaaga ggctgggaaa a
//
LOCUS       DACCXW010000013        34346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCXW010000013 DACCXW010000000
VERSION     DACCXW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.013"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            130..810
                     /locus_tag="IVY71_000524"
     CDS             130..810
                     /locus_tag="IVY71_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7984597.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            900..1934
                     /locus_tag="IVY71_000525"
     CDS             900..1934
                     /locus_tag="IVY71_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7984598.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1948..2283
                     /locus_tag="IVY71_000526"
     CDS             1948..2283
                     /locus_tag="IVY71_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984599.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2267..4747
                     /locus_tag="IVY71_000527"
     CDS             2267..4747
                     /locus_tag="IVY71_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984600.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            4803..6362
                     /locus_tag="IVY71_000528"
     CDS             4803..6362
                     /locus_tag="IVY71_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7984601.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6359..7723
                     /locus_tag="IVY71_000529"
     CDS             6359..7723
                     /locus_tag="IVY71_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7984602.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7894..8964
                     /locus_tag="IVY71_000530"
     CDS             7894..8964
                     /locus_tag="IVY71_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984603.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            8979..9647
                     /locus_tag="IVY71_000531"
     CDS             8979..9647
                     /locus_tag="IVY71_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984604.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9667..10245
                     /locus_tag="IVY71_000532"
     CDS             9667..10245
                     /locus_tag="IVY71_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7984605.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10646..11557
                     /locus_tag="IVY71_000533"
     CDS             10646..11557
                     /locus_tag="IVY71_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7984606.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11645..12562)
                     /locus_tag="IVY71_000534"
     CDS             complement(11645..12562)
                     /locus_tag="IVY71_000534"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7984607.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12764..13201
                     /gene="zapA"
                     /locus_tag="IVY71_000535"
     CDS             12764..13201
                     /gene="zapA"
                     /locus_tag="IVY71_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7984608.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13260..13808
                     /locus_tag="IVY71_000536"
     CDS             13260..13808
                     /locus_tag="IVY71_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7984609.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            13923..16283
                     /locus_tag="IVY71_000537"
     CDS             13923..16283
                     /locus_tag="IVY71_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7984610.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16370..16684
                     /gene="trxA"
                     /locus_tag="IVY71_000538"
     CDS             16370..16684
                     /gene="trxA"
                     /locus_tag="IVY71_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7984611.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            16866..18644
                     /gene="uvrC"
                     /locus_tag="IVY71_000539"
     CDS             16866..18644
                     /gene="uvrC"
                     /locus_tag="IVY71_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7984612.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            18789..19688
                     /locus_tag="IVY71_000540"
     CDS             18789..19688
                     /locus_tag="IVY71_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984613.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19681..20943
                     /locus_tag="IVY71_000541"
     CDS             19681..20943
                     /locus_tag="IVY71_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984614.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21104..23002
                     /locus_tag="IVY71_000542"
     CDS             21104..23002
                     /locus_tag="IVY71_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP7984615.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23332..24051)
                     /locus_tag="IVY71_000543"
     CDS             complement(23332..24051)
                     /locus_tag="IVY71_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP7984616.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24388..24720
                     /locus_tag="IVY71_000544"
     CDS             24388..24720
                     /locus_tag="IVY71_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984617.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24707..26710
                     /locus_tag="IVY71_000545"
     CDS             24707..26710
                     /locus_tag="IVY71_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7984618.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26729..27199
                     /locus_tag="IVY71_000546"
     CDS             26729..27199
                     /locus_tag="IVY71_000546"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7984619.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27220..27804
                     /locus_tag="IVY71_000547"
     CDS             27220..27804
                     /locus_tag="IVY71_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7984620.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            27819..28805
                     /locus_tag="IVY71_000548"
     CDS             27819..28805
                     /locus_tag="IVY71_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7984621.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            28795..29106
                     /locus_tag="IVY71_000549"
     CDS             28795..29106
                     /locus_tag="IVY71_000549"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7984622.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29128..30909
                     /locus_tag="IVY71_000550"
     CDS             29128..30909
                     /locus_tag="IVY71_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7984623.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            30902..32278
                     /locus_tag="IVY71_000551"
     CDS             30902..32278
                     /locus_tag="IVY71_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7984624.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32285..32920
                     /locus_tag="IVY71_000552"
     CDS             32285..32920
                     /locus_tag="IVY71_000552"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7984625.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            32950..34305
                     /locus_tag="IVY71_000553"
     CDS             32950..34305
                     /locus_tag="IVY71_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7984626.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        11356 a         5917 c         7370 g         9703 t
ORIGIN      
        1 cgattttatt gtttttttct cttttataca aaaagggtca taatttcaag taagcggatg
       61 aattcatgag gattttttgc atactgtctc tactatactt aggtcactaa gaaaaaggag
      121 tgatttatta tgaaaaagaa catgattggg acattattac tttcaacact tcttctaggc
      181 ggcttggcaa caccagcttt tgctgagggt caagcgactt caaaaggaga tattaccttt
      241 actgaaccaa caaataccgt ggagcctctt aaccctactg atccttcaaa accagtagag
      301 ccagcagatc cggaaaatcc agcaactggc cagacaggtc ctttaacact ggatgtagtt
      361 cccgaattgc catttggtac acacgagatt gaaagcggaa caaaaacgta tcaagtagac
      421 gcttcaaaaa acgatacacc ttatctccaa gtatcagatc gtcgaggtgt aggtgcagat
      481 ggccaagcac aagggtggaa cgtgacagtt tcggtttcag attttgtaaa cggttcacaa
      541 gttttgcaag gcgctgaatt agattttgga acatccactg ttaaaagtac gtcagataac
      601 gaatccactg gaccaactag tcaaactgta actgggttga gtaaagcaag tgcagcgaca
      661 ccaatattta cagcagcaaa agatcaaggt ctgggaacat ggctttctgt ttatgatcca
      721 gcaaatatca cactgaaagt gccgaaagca gcagctggaa cattcacggc tgatttgact
      781 tggaacctcg tggctggccc agtagcatag agtatgaaat aaattcaaca aactgcggtg
      841 aaaatactat aggtgaagga ttttttaccg cagtttttgt tgaaaggatg tggtggacaa
      901 tgaatcaaag taaacgactg tttgtttcgt ttttttctat tttaagtcta tttttcattt
      961 ttccgtcaat cagccaagca aaggattcag caggagactt tggaatcaaa ccggtatttc
     1021 cagagaacca aatagataaa gcaatcggtt attttgattt attagtggca ccggaacaaa
     1081 atcagacact agaagtaatt atcagcaatt cttcagatga agaacgaaca tttgaggtct
     1141 ctgtcaatcc ggcggtaacg agtgatggtg gaacgatcga ttatagtcaa aaaaacccaa
     1201 cgttagatga gacattgccg tttgatgtcc gtgacgtttt gctcattgcg aaaaaggaaa
     1261 tcaatgtatc agcacatgca gaaacaactg tacctataga agtgaaaatc ccagcaaaaa
     1321 gctttaaggg acgtgtatta gcgggtattc atgtttcacc taaagaagaa gctgaaacag
     1381 aaaatgctac agaaggagca caaatcaaaa atcgtattgc ttataatctc gcagtcgttt
     1441 tacaagaaag tcaagagacg atagaaccgg atctgaaact tttatcagga gatttggacg
     1501 aggtcaacgc aaagccaact gttcagcttc gtttccaaaa tcctcagcct aggattatca
     1561 gcaatttgat atttacgagc aagatctttt atgaaaatca gctttatatt gaaaatacgt
     1621 ctaatgcatt tttagttgca ccaaattcga attttcactt gaatcttgat ttagctggtg
     1681 ataaagcaaa agccggcgat taccgcgcag aaatcatagc aaaatcaggc gacagtaatg
     1741 aatggcgatt tacacaaaac tttacgatca aaaaagaaaa agcacaaaaa gtaaatgaaa
     1801 attccgtttt tgcagtacaa gaacaatctt tcccatggct ttatgcagga ttggttgctg
     1861 cagttctagt gttatggttg attttgctgt ttttgtgggt ccacaagaaa aagaaaaaag
     1921 gagaaacaac atgatgaggt gatttcgttg aaaacatcga tcttattggg agggattatt
     1981 gcaagtttgc tgttcttcag ctggcaagag ccagtccaag cagataccaa aagttcaggt
     2041 acgattacat tttatgatct aagtccaatt ccgcatccaa gtggttcagt caatggcgca
     2101 tcaaaacagc agacacagct accgatttat aaagaatcta atcaacatgg cgtattcccg
     2161 gagacgaatg aacaaaagat ccgttttctt tatttgatca ttggtataga attatggctt
     2221 cttgcgtggt tgttgttatg gagaagaaag gaaaaggagg atgaacatgt tgctacaaaa
     2281 taaattttca ttttttcgct ccttcttttt tacttcgctt tttgttttaa gtatatttat
     2341 cttttcacct tcggcagaag ctgttggagc aacgcctcct gccatttctt atgatccata
     2401 cacgtatgaa aatatcgtac ctccaaatgg tggacgtagt gcagtcaatg gcgggaagct
     2461 tgtccaacta actggggcta accaattgcc taaattgact gctacttatt tgaacgatgg
     2521 ttcaaatgat ccttggaaag gtggcacggt ccgttttcat tgggaaagcc atgtgaaact
     2581 aacaaatgta cttgatttgg tgaatatcac tgcacaagga tttgcatttg tggcgatcat
     2641 caatttgccg gaacatgtat caagcagtga tgtattaaaa gcaattgact ggaacaacgc
     2701 ctacttgtat attgccggtg cccccatcaa ggttactagc ggaagtttat tatcagcagg
     2761 aagccatacc ttgcgttttg ggatggggtc gtgggataca aatactctat tgacagatat
     2821 tattgctatt attacgcgag gcgaattcaa cgtggacgat atcccgcttc aatttgatgt
     2881 tacagctaat gtcgcgaaga tgacagaaaa cggagataaa tatgatacga gtccaaacaa
     2941 aattttgacc aagggtaaat tacaaccttc tatgtctaaa aaggccgttt tttctgttga
     3001 tttttatgat tcgaataata ttatccaaga tcgtatgttc aattcgattt tagctccagc
     3061 tgcaggtaga ttttatccta aattaactgg cggaaacttg aatccaggag catatgcatt
     3121 ttctgcaagt gcagagataa atacgtggaa tagttacatc tcaccaagtg attcaacagg
     3181 aaaatataat acatttgctg ataccacaga atacgtggat ggaaataacc agaaccttcc
     3241 aggctctacc aatagtcgga cattgacgat ggggctgact gaaggacgtt ttgatcagtt
     3301 gccatttagt cgctttgatc gcgtagtcaa ctattttaca ggaaaaaacg ttacacaagg
     3361 agcaatactg actcatactc cagcaagcac aagtggattg aatcagacag ccaatatcgt
     3421 gtacagtggg aaagatgcgc aaggcggatt gctttctcct gttgctttaa aggtattgga
     3481 gcagtacaag ctagacggaa cggttttacc aacaaactta acagccgata gtcagtgggg
     3541 aaagctcctt tcttctgcat cgtaccaagt cacggccaat tggtcagcaa ctgaactaaa
     3601 aaagggttcg atccattaca ggctttattc gaaaggaacc caaaaattag cgacaggatg
     3661 gacagatcaa cttttccaaa cgatccaatc aaataacggg ggtgtaaata cagctacttc
     3721 tacactacct gcattggctt cagggcaata ttattttgat tatcgcttgg tggacgatgt
     3781 acttactaac atgtatccgt cttttgcgta caaatggcag tctgaacaag ctggaatcac
     3841 cactcttcct caaataacag tggctaattt tccatctgtg agtgcagata gtcagctaaa
     3901 aaaccttagc agacaacctg agacaggaga tcccctgtct gctctatcag gagatacgat
     3961 tcaagaaaat gtgacattca cgttaacaaa atctggggat gcgataaccg acaaaaaaat
     4021 cacgatctca cttcctgaaa atactactta tatcaatggt tccttaaaat taaatggtac
     4081 aactatcgca gataccggca ttcaacaagg agtttctgtc ccagcagatc tattgagcaa
     4141 aataggagat accattcatc ttacttacaa ctatcaatta aatactgtag atacttcttt
     4201 acaatccgtc agcatactga caaaggcagc tgtactttcc agcaatatta cattagcaga
     4261 tggtgctaag cttcctaacc cagttgtcca aacatctgct aaaacaattc ttgtccccaa
     4321 acaagaatta acattagtta atgtaccaga tgattttaca tttggtaacg atctccccaa
     4381 accgttgaag acaagttatt atgaatcaaa aggcgatttt tcttttgatg tgcgggatac
     4441 tcggcttcca agcacgtctc cttggcaact gacaggtaca ttgacttctt tatttaagaa
     4501 tgatcaaggt caagaattaa gcggaacaaa attatacttc aatcattctg gatcaaagca
     4561 attgattcag caaggtcaaa acacgttgat ttatgagagt gatggaacag ctaaaggaga
     4621 ggttctcgtt gactttcccg ataccgatgg tttactctta gaggtaaata gcagtacgaa
     4681 tgctcaacct ggcgcaacgt atcaaggcat ggtcacatgg aaactgacag ctgggccaac
     4741 cagctaagtg attagaattt gtatggatct gtgataactg tcacagatcc tctttttcaa
     4801 ggatgagaag gaaacttctg ctgttaaaat cagaaatgct tgaaggagtg gacttggaac
     4861 ttttcagttt gcttgaaaaa aatgaacaac ggcaaatcaa acttttccaa cttttattag
     4921 aggaaaaagg agaggtttcc gttaataacg tacttacccg cttatcagtt gatcgggcta
     4981 ccttgaaaga agatctgttt tatttacaag ctaacttgga aaatatcaaa caattggaaa
     5041 ttgttatcca agaagggaat attttattaa tcagacatgg aaatatagca gcagtcgatg
     5101 tttattatca ttgttacttg cgctattcta caaaatatca gatacttctt tatctgttgg
     5161 aaaatggctc atttgatcgg cagaaacttg tcatggaact taatatcagt tcggctactt
     5221 tgtctcgcag agtgaaggaa ttaaatgatt ctttaaatga gtttcatctg aaaataaaaa
     5281 gcgggcggct gaccggtcca gaaagtcaaa ttcgttattt ttatttccaa ttattttggt
     5341 ttggacgtcc ctatcttgaa aacaaaaaag aatacagtga tttatcagga aacacttttt
     5401 tagatctgct gcaaaaagag tttagttttg ctattacaga agaaggacga ttgaaatttg
     5461 aaatatggct ttccattatg aagaagcgaa tgcgctgtac gaaaaaatcc aaaccagatt
     5521 atagcaatct tcctgttgga ttgtttcaaa cgacacaatt tttattggac ctccatacat
     5581 ttttgacccg ttacttcttt caatatgcct ttccttggaa tcaaaatgaa acgttggttt
     5641 tttatctttt catgatcagt aactttacat tagatacaca taactcgcat gtccaggagt
     5701 ttttgataag cgaaaaatac aaagacgacg tagtagctca attgaatcga acgtttaaac
     5761 aatcgataga agaatatttc tctttttatg tttttccaga agagatttcg caaaagattg
     5821 cgttgacagt gatgcaaaat cacgctcatt ttgcctattt tagaggatgg atgaatattt
     5881 ttggccaaga aggattaaaa cagcggttaa ctgaagtaga agataaacaa ttcctggttt
     5941 tatgtgaaag attggtcaga caaacatttg ctattgttga ttttactgaa gtagacgagg
     6001 aaaatgcccg ctttgaatta tttgctcgct atgcatctat tttacggacg ctgatcaagc
     6061 agttagaatt acgtctgcaa attgcttatg atcttcctta tgaaaggcta atggcggata
     6121 tcgtgaaaga aatcatccag gaacaattgc tgaatacgat caaatatgat ttagtcgaat
     6181 acgagaaaga aaagcagtat gatatcattt tgactagcca attgaaagag tacccgtcac
     6241 agaagtccgc taaagttttt gtattcacta gcaacgaata taattatgat ttcccctatt
     6301 tgaaccaatt tttaaaagaa tgttatttag aaaaattgaa tttaaggatg aataaaacat
     6361 gaaaatcgtg atcattggtg cttcccatgc aggcatcaca gctgccctaa atctaagaaa
     6421 acttcaaccg aaagcagaag ttttactgat cgatgaagat cataaagatg gactaggcta
     6481 tgtttcaaat ggaatcaacc tttatttaaa aggaaagata cgtagtttag ctgaagttgc
     6541 ccataacatg cggactcttc aagaaagtgg ggcaaaactg atcaccgaat ggagagtaac
     6601 tgaacttgat ccagacaaac atcagctcat cctcgcatcc aaagaaggaa aaaacgagac
     6661 cataacctat gataaattaa tcgtagcgac aggttcctct cctgtcacct tgtataaaca
     6721 aattgaagct gaaaatgtct acacctataa aaatctagtg caatctaagc aagtacttgc
     6781 cgctttaaaa gaagcaaaag aagtagtgat cttcggtgct ggttacattg gattggaatt
     6841 agcagatgct ttgagaaata aaggatacat tattcatcta gttgattata tgccgaatgt
     6901 tttatcaaga tatttcgata aggatatgat caacagtttt caaaatcagc tgcaaactaa
     6961 gcaaataaat ttttatccaa atgaattctt gatcgattgg aaaaaatcag aagagaaagt
     7021 agtatccgta caacttctgt ctcaaacaat aaaggcagat atggtcattt tttctgccca
     7081 aactcgtcct aatacgacat tgttaaaaga aaaagtggct ttgtatgagg atgagactgt
     7141 gatagtcaat gaatatctgc agacaagtga tccggacatc tatgcgattg gagatatcgt
     7201 cccagtcagt tttgacaaaa ataaacggca tctcttttta cctttagtta ctcgagctgt
     7261 acatatggca agagcagtag cgctaacttt gagtggtcag ccaaccgctt acgacctcag
     7321 acaaaagata acagcagcag tcattataga ctacttttta ggaactgtag ggctgacaga
     7381 agatgaagcg ccattcttgg aacaaaatac cggttcgtgt tcaggagaat ttgatctatt
     7441 tccacaatac gacgaagaaa acaaaacagt aaatgcaaag ctgatctacc atccggatac
     7501 attagaaata attggcggac agctgataag ccaagaattc ttattatcag atttgaacct
     7561 gcttgcggat atcgtgaaac acaaaactac gattccacag ctagcagttg aggattttgg
     7621 atttctgtct gaatacacct ctcgttttca ctacctcaat gagttagctt ttaaagtact
     7681 agctaagaga gcaaatagca atagagtaga taaaagactg tgagaaatat cacggtcttt
     7741 tattgtgtgg ggatataata aatattaatc aatcgagaat aaaaaattca tataatgaaa
     7801 tagcggaaga attttttgag cataatattc gccaaaaatt tatcaagtga taaaattgtt
     7861 gtataaaatg attatttcag gaggaggagt taaatgtttt tagcattaaa tgaaattatg
     7921 cattcaaagt tacgttatgc gttagttgca ggggtgatgt ttttgatcgc ctacctggta
     7981 ttttttttga ctgggttagc ttatggctta gctcaagata atcgaacagc agtcgacaag
     8041 tgggaggcag attcgattgt gctttcgaaa gatgcaaatt caaatctagg gatgtccatg
     8101 attactaaga agatagcaga agaagtcgaa ggtgggaaag tggcgtatct tgcccaaaca
     8161 cctggagtcg tgactagtaa agacagtaca gaagaaggaa aaatcaatgt tagcttcttc
     8221 ggtatcgata aaaatcaatt catcatgccc aatcttgttg aaggaaaagc ttttgataac
     8281 gatgatgagg cagtaggcga tattagtttg aaagaagaat atggtctagc cataggagat
     8341 acagttaagt tatctggtag tgacaagacg tttaaactaa ctggctttac ggatcatgcc
     8401 aaattcaatg tctcaccagt tctctataca accatcaatg cttaccaaga aatccgcttc
     8461 gaaaaagaag atacaagtga aaatgcacgg attaacgcaa tagttgtcag aggtaaaatc
     8521 agtaatttac ctgaagattt agaacagatc aaaatttcta aattcatcaa tgaattacct
     8581 ggatataatg cgcaagtatt gacttttgga tttatgatcg gatttttgat cgttattgcg
     8641 gctattgtga tcgggatttt catttatgta ctgacgatgc aaaagattaa tatctttggt
     8701 gtaatgaaag cacaagggat cacaggagga tttattgcgc gttctgtagt ggcacaaaca
     8761 ttcattttat catttgttgg tattcttctt ggtctattag gaacagtggg aacatccctt
     8821 gttctacctg atgcggttcc attccaaagc aattggctgt tttttggcgt cattagcttg
     8881 ttgatgttag tagtagctgt gttaggcgca ctattttctg ttcggacaat cgtaaaaatc
     8941 gatccgttga aggcaattgg ataagaagga ggaagaacat gaaagcaata gaatttaaag
     9001 aagtagaaaa aagtttccaa gatggcgacc aagtagttaa agcattgaaa gaaacaacca
     9061 tgagtatcga taaaggggaa tttgttgcaa tcatcggtcc ttctggttca ggtaaaagta
     9121 cttttttgac tttggcaggc ggattgcaga cgcctagcag tggctctgta tttatcaatg
     9181 atcaagaatt cagtaatcaa aaagaaagcg aacgcgttaa actacgtttt aaagaaattg
     9241 gctttatttt gcaagcttcc aatctggttc cttttctgac agtaaaaaag cagttattat
     9301 tagtagataa gattaaacat gaacatcgtc aaaaagaagc aaacgaatta tttgagcagc
     9361 ttggcgtgga taagttgatc aacaagtatc cagaagaact atctggtgga gaacggcaaa
     9421 gggtcgcaat cgctcgtgca ttgtacaatg atccgtccat tattctagca gatgaaccaa
     9481 ctgctagttt ggattcagaa aaggcacgag aagtagtcga tatcttggca aaagaatcaa
     9541 aagacaaaaa taaagcaacg atcatggtta ctcacgatac acggctgatt gacaaatgcg
     9601 atagaatatt tatgatggaa gatggtgtat tgaaagaaaa agaataacca aaaagcaggt
     9661 ggaaaaatgg tccatcgata caacaaagat acacagcaaa aaattttgaa agaagcgact
     9721 gacttgttta tgtccaaggg atatctcggc acttcaacaa gagaaatcgc acaaaaagtt
     9781 gggatcaccc agcccaatct ctatcattat ttcggtgata aagaaaaact atatacggca
     9841 gttttagaaa accacttaaa agatgttgga aaagctttga gagaaatcgt tcaaacaagc
     9901 gaaacgggat ttcaagtaac cttgacaaaa atggcgcagt ttttgattga gacacatctg
     9961 gtcgatttgt ttctgatgct tcacgatctt gaaagcaatc tttcaaagga aacaagagat
    10021 catcttttct ttctatggaa gaaaaattat cgtgaacctt ttgaagaaat cttttcagca
    10081 aaccaatctg tgctaagaaa tgggatttca aaagaaatcg cagcaaggca ttttttctta
    10141 gtgttagcac cttacatcac tcggtcgaaa agacctgtgg aagaaccgct aaatgtggcg
    10201 caattgatcg atttatattt gaatggtgta ttaggagaag cgtaaagctg ttatataggg
    10261 agaaaatagt taggtgaaga agcaagatat gacgaagatc gttatatctt gcttctttat
    10321 ataggtagtt tttcataaag acaaatttgt tattctctca tgattatcag ggtatatttt
    10381 tatttattat gtcggtgttc caggaagtgt tttaatttaa taacgaagaa attaattgtt
    10441 tgtagaatga aggaaactat ttattgggta gatgaatcac cacgaatagt caaaaggaga
    10501 tttcaggata tgccaaaaat gaaaaaaatc attttagcag taatgttatt ttctacggtt
    10561 atatctttta cagccccttt tttgcttact taagctgatt ttaagttttg gcccttagtc
    10621 tggatctacg aggtgagagt caagaatgaa aaaaataatt ggagcttttg tatttgcaat
    10681 cattatcatt attatctgtt ttcaaataga tgtttttggt caaaaaaaga tagttggaca
    10741 aacggtcagc agtacgaaca gtacggttac aaagacaaag gaatcatttg atgatacaga
    10801 acaagagaca ttaatcaaaa tcggaaaaaa tgaccgtaca ctatatacag atgactcgct
    10861 aactacccca cttgcaacta taaacggcgg agagctggcg gagtttcttt ctgaaacaaa
    10921 aaatgcttat cagatcaaga caaatgatgg ctatactgga tatttagccc tcgaagatgg
    10981 aaccaaagtc acaaagaata tacaaacaaa accggaagag ctatctgatg cagtgattgt
    11041 attagatcct gggcatggcg gaaatgatac tggggcatta agcaatgatg aacaaacaga
    11101 agaaaaagat ataacattaa gtacagccat aaaagtaaaa aaggcgctag aggatgctgg
    11161 agcaacggtt tatttgaccc atactacaga tgaattagta cagcttggag atatatgcga
    11221 ctacagtgaa gaaaaaaagg cagatgtctt tatcagttta cacgcagact ctacggaata
    11281 tgcgaatgaa gcaactggaa tcacgacata ttattactat ggacaagagg aaacattagc
    11341 acagacaatt gctgactcct tcactgatct gcctctggat tcaagaggaa tctccactgg
    11401 gaactatcaa gttcttcgag aaaatcttca accttctatt ttaatcgaga tgggttatat
    11461 gaataatgat agtgatttaa aggaattagt aacagacagt tatcagcagc agattgctgc
    11521 aagtttgaca caagcattga ccacttattt tcagtaataa ataagcccag ccaaacaaaa
    11581 atagctcctc atatttttcg tttggccggg cttattatcg cagatgcaag cttttgaaca
    11641 ccgtttattt ttttagtagc ttcaaagctt tttctgtatt tgcaaagtga agtttggcat
    11701 atttttcaag cataggcatg ccgcctttct ccaatagttt tgccgctact tggtcagatt
    11761 tcgttccagc tcctgatgga agggtgattc cagcttcagc tgatgctttg tgtaactctt
    11821 ccaaaaagct ggcacgactg atgatagaag cagcagcaac agctaaatga tattgttctc
    11881 ctttagtaac gaagtacagt ttttcttgta attgattttt ttctaaacgc gcatattttt
    11941 taaaatttgc ttccggcgta aactgatcga tcaaaatcgc ttctggtttt tctggctcga
    12001 ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc gactttcatc cgaacggcat
    12061 tgtattttgg ttggatctca ttgtattttt ctggagataa gacaagcagt ttatagggga
    12121 tggttttttt taagatgtca gcaagctgga tgatttggct atcttttaac tcttttgaat
    12181 ctctaacacc aagtttacgt aattctgcaa gctgatcacg tttcacataa gcagcacata
    12241 ctgttagcgg accgaaatag ctgccatttc cgacctcatc actacctaaa acggataatt
    12301 gactgatatt ttttggcaaa gaagaggctg tttttgctgg cttggcttgt ttatcctgtg
    12361 cctcccccca tttatcagct tctattgctg cattcgtacc ttgaaacatc acttttccgg
    12421 atgtataagc ggtaatggtc gtcccgtctt tttttgcagc aaagctagta taaggaatct
    12481 gtttatttaa caaataagag cgatagtatg ctttcatttg actaagttgg tcgtttgttg
    12541 ctttgataat gacgttgctc ataaaattct cctcgttttt atacataaaa ctataccgca
    12601 actcacgact tttcgttttc taagagattg cgagacatag cattttttta aagtttatca
    12661 tataatagtt aagtatgtga aaaatatttg tggagaacgg tgaaagaacc aacttcatgg
    12721 tacactaaat aaaactttca tcaaatagga ggaaatgatt cgtatggcgc acgaaaaaac
    12781 acgctacaaa gcggtgattg ccaaccaaac atatactatt atcggacggg aaaccaagca
    12841 tcatatggat atcgtaacga agctgatcaa tgaacagtta gcagaattga aacaattatc
    12901 accacaaatg gataatgaac aagcagcaat cttgatggct gtcaatgcgc tttcggatca
    12961 attgaagaaa caagaaagaa tcttggagct ggaagaagaa acagcagaat taaagaaaaa
    13021 aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc attgaagcaa tcgagaacga
    13081 agctcgggaa gtattgaaag aaaatggtca agcagatcat accattaaaa atcatgtgga
    13141 agcacaacag attttaaacg agaaacgaaa aggacaaatt aaacaaaaat cttctaatta
    13201 agatcctttt atcaaaataa gaaaaattta aaaaaagtta gaagagaaag gaacgtagca
    13261 tgctgagttt gctgatttta tttttattgc taattgcgtt tttttctgga gcacgtcgcg
    13321 ggtttgcttt gcaaatcata ttcgcgattg gatatgtatt gtcatttatt gctgcccagc
    13381 atttttacaa accattagca agtcatttgg aattatacat tccataccca gctgtcacgc
    13441 caacatcaaa gttagcattt tttgatcagg tatttgcctt tcacctagat gaagcttttt
    13501 atgcaggaac tgcttttctg attattttgc taattggctg gctgcttacc cgatttgtgg
    13561 gggtttttgt ccatggattg acgtatgtgc cgatcttgag acaggtagac tggattgcag
    13621 gaggcatcct gtcactaatc atggcttacg ttactatatt tttgattttg cagttacttg
    13681 cctttgttcc attagatgtg gtccaaaatc aattcggcga gcatagtttc gctcgcttta
    13741 tcgttgagag aacgccattt cttaccaaca agatgtatga gctttgggta acgaatattt
    13801 tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt gtacagagta gattatttaa
    13861 gaaggctgcg tagcgtatgt ccggcacagc tcttttttca tagtgaaaga taaggtgata
    13921 aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag atggtcagac
    13981 aatttgtagt gactgcacaa ggaaaagaag aactggcaga attggtacca gtgagcgagg
    14041 aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc
    14101 gaggcgggat ccctatacct aaacttgaaa atatccgtcc ccatatgaaa cggattgaga
    14161 tcggagcaga cttgaatgga gtggagcttg cacaagtttc cagagtattg tctacgacaa
    14221 gcgaattgaa tcgtttcatt gatgatcttt atgacagtga aatcgaattt gcacgtttgt
    14281 atatgtgggc agatcagtta gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa
    14341 tcgatgaaga cggacgcgtg actgatgacg cttctcctga gttaaagagc ataagacaga
    14401 acattcggcg aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa
    14461 atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac gttatcccag
    14521 tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt gcttcaggac
    14581 agactttatt tattgaacca aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc
    14641 aaatcgctga acgaaatgag attcaacgta ttttaagtga actttctgct gaacttgttc
    14701 cccatcgtca agaaatcatc cacaatgctt atgtgattgg taaaatggat ctgatgaatg
    14761 caaaagcacg atttggtaaa gaagtaaaag ctatcgtgcc gggaatcagt gaagacaatc
    14821 atgtcgtctt gaaacaagca agacatccac tgatcgatca ggaaaaagta gtttctaatg
    14881 acatcacaat cggaaaagac tatcaagcca tcgtcattac aggacctaac acaggtggga
    14941 aaacgatcac gttgaagact ttaggtctgt tacaactgat ggggcaagca ggattaccga
    15001 ttcctgcagg tgaagaaagt caaatcggta tttttgagga agtatttgcg gatataggag
    15061 acgaacagtc gattgagcaa agtctatcga ccttttcttc tcatatgacg aacactgtag
    15121 atattttatc aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa
    15181 cagatccgca agaaggtgct gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt
    15241 ctgcttatgt gatggcaaca acgcattatc ctgaattgaa agtttatgga tataatcggg
    15301 caaatacgat taatgccagc atggagtttg atgtggatac attaagtcca acgtatcgtt
    15361 tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg
    15421 atacagaagt tattgatgaa gcgaagcagt tgatgaatga tgagagccaa gatttaaacg
    15481 aaatgatcac agatttagag aatcgccgga aaatggcaga gaccgaatat ctggaaatgc
    15541 gtcattttgt ttctgaggca caagaacttc acgatgattt gaaagaagct tacagctatt
    15601 tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaat gaagtcgtgt
    15661 cagaggcgga agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga
    15721 ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc
    15781 tacatcaaga agaaactttg aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa
    15841 cgttgaaacc gggagacgaa gtgttggtta cgacttacgg ccaaagaggg acgcttttac
    15901 gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag
    15961 acgagctgac gcctgttgct cctcaaaaag agccgacaca acgagttatc catgctgtac
    16021 gatctgaatc tagtagtcac gtgccgaatc aattggacct tcgaggaaaa cgatacgaag
    16081 aagcgctaag tgaggtagat caatatttgg attctgccat tctagctggt tatcctcaag
    16141 tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa gggaattact gattatctta
    16201 aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg
    16261 cgacaattgt taaatttaag taaagcttac taaaggtaag cagatggttc gctatccaag
    16321 tatagaaatg ctatactagt catagatttt aaaaatggag gtctttgata tgtcacaagt
    16381 aattacagat aagacattta atgaagaaac agataaagga cttgtattga ttgatttttg
    16441 ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt ttggatcagt tggaacaaga
    16501 atatgatgaa gaagaattca gaattgctaa aatggatgtg gatgaaaatc cagaaactcc
    16561 tcaacaattc ggtatcatga gcattccaac attaatgttg aaaaaagatg gtcaagtagt
    16621 agaaaaagct gttggtgttc attcaaaaga acaactacgt caaatgatcg atcaatattt
    16681 ataaaaactt acagcaaaaa taagctgagg gtgggactga atccagttcc gtcctcggtt
    16741 tttttgcggt tgagttaagg ttattcaaat taatattttt tgagtataat tacgttgtca
    16801 ggctggattg tttttttgac agacatgcta taataacttt aattgaatat gaaggggagt
    16861 accagatgaa cgaacgaatc aaaaacaaat tagctttact tcctgatcag cccggctgtt
    16921 acttgatgaa agataaaaat ggaacgatca tttatgtcgg caaagccaaa atattaaaaa
    16981 atcgtgtccg ctcatatttt actggtagcc ataatacgaa aacggaacgg cttgttagtg
    17041 aaatcgaaga ctttgaatac atcgttacag aatcgaatat cgaagcattg ctcttagaga
    17101 tcaatctgat aaagaaaaat gatcctaaat acaacattat gttaaaggat gataaaacct
    17161 atcctttcct taagataacg aatgaaaaat atccccgttt ggtgattact cgtaaggtac
    17221 taaaagacaa agcactgtat tttggaccat atcccgatgt cggtgcggcg aatgagacaa
    17281 agaagatcct cgatcgatta tttcctctga gaaaatgcaa acctagtcag acgaaggagc
    17341 cttgtcttta ctatcatttg ggacaatgtc tgtgtcctta ttatttcgac gtagatccga
    17401 aagtctatac gaaaatcgtt gatgaagtga aaaacttttt aaacggagga cacgaaaaaa
    17461 tcgagtcaga gatccatcag aaaatggaaa aagctgctga aaacatggag tttgaaaaag
    17521 ctgcagaata ccgagaccag atccgtgcca ttgagacaat catgactcgc caaaaaatga
    17581 caaatactga tttactggat cgagatgtat ttggttatgc gattgataaa ggatggatgt
    17641 gtgtccaagt gtttttcgta agacaaggaa agctaattga acgagatgtt tcaatgtttc
    17701 ctttctataa cgaagcagaa gaagatttct tgacttacct cggtcagttt tatcaggaaa
    17761 atgagcattt cattcctaaa gaagtcttga ttcctgatac gatcgacaag cccagtgtag
    17821 aagctttgtt agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa aaattagttg
    17881 aattggcgag taagaatgcc tcagttgctt tgaatgaacg gtttgatctg attgccagaa
    17941 aacaggaacg gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc
    18001 caatacgtat cgagtctttt gataactcta atatcatggg gacgaatcct gtggctgcga
    18061 tggtcgtatt cattgatgga aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa
    18121 cagtcaacgg accggatgat tatgcatcaa tgagagaagt catctatcgc agatattcgc
    18181 gagtgattcg cgaagggttg cctttacctg atttgatctt gatcgatgga ggaaaaggac
    18241 aagtagatgt ggcaaaagat gtcttagaaa atcaattagg aatcgatatt cctattgctg
    18301 gcatggcaaa aaacgataaa cataaaacaa atgagttgct attcggtaca gatttggctg
    18361 tagtaccgct tgagcgaaat tcaccagagt ttttcttact ccagcgaatc caagatgaag
    18421 tccatcgatt cgcaatcact ttccatcggc agctacgcag taaaaacagt tttgcttccc
    18481 gattagatga aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca
    18541 aatcgttgaa aaatatcaca gcagccacga cagaagaatt acaagaaatc ggactgccga
    18601 aaaatgtcgc acaaaacgta tttgataaat tacatcagga atgaggaaag ggcactttac
    18661 aaagaaagta aagtgtccta tcattttatg tacaacaaaa agagcgttta cgttttgttg
    18721 gtaaataata aatcgcttac gtctggtgtc taaggatata tgtgatttct tgaagaagga
    18781 gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt tgggaactac acagctgtcg
    18841 atcatgtctc tttccaaatc cccgatggaa aaatcatggg gctaattggc caaaacgggg
    18901 ctgggaagac aacgactttt cgtttgatct tggatttttt gacacaagac caaggagaag
    18961 tgttatggaa tggccatcat cttggggaga aagattatga tgtcatcggt tatcttccgg
    19021 aggaacgggg actttatccg aaagtaacga ttcaagacca actgctttat tttgcagaac
    19081 ttagagggaa atcaagaaaa gagattgaac caaaaatcga cttttggatg gaaaaatttc
    19141 aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc taaaggaaat cagcaaaaag
    19201 tccagctgat cgccacgtta atccatgaac ctaaattgat cattttggat gagccattca
    19261 gcgggttaga tccggtaaat gctgaattgc taaaagatgg aatattggaa ttgaaaaaac
    19321 atggttcatg tgtcattttt tctagccaca atatggataa tgtagaaaaa atctgcgatc
    19381 atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg
    19441 aatcatttgg ccgtacgaag ttattcttgg aatctggttt aagccaacag gaagtagaag
    19501 aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag attaccttag
    19561 aagatcctga agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc tacattccta
    19621 tgtttaacca gcaaccgcca actttggaag agattttcaa attgaaagca ggtgaaatcg
    19681 atgcgtaagt tttggattat taccaaagat gtctatttaa aaaatgttaa gtcgatttct
    19741 ttcttgatca tgatattagt cccttttatc atgctgggta tcatttatgt agcaggaaac
    19801 tttgctcaac aaaatagtga tgtcaataaa atcggcgtat tggctgataa tgagcaaatc
    19861 agtcagcaaa tgagccaagt caaaacagat gattttgagt tcaaagcagt tgattctgaa
    19921 gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt agtctcaact
    19981 gagaatgata agataaatgg gatgctttat agcgaaaatt cattaggaca aagtacacaa
    20041 ctactgatac aacaacaact gactggcttg caatcgatgc ttcgagcgag tcaattaggc
    20101 atttcaccag aagaagtagc tagcttgagc caacaagcaa gtttgactcg acaaaaagtc
    20161 aattttgatt caaaaggaaa aatgacagtc ggagaagata actcgacagt ccaatacgtc
    20221 gtaagttatg tagcaacgat tgtcttgttc attattattt tgacgtatgc gcagatcatt
    20281 gcacaagaga tcgcttctga aaaaggcacg cggatcatgg aagtgatcct gtccagtacg
    20341 actgcacaaa agcattttta cggaaaatta acaggtgttt tacttgttgc attaacgcaa
    20401 atggcattat acggtattat ttttggtata ggatacagtc aattcaaaga tatggaaatg
    20461 gtcaaaaata tattagacgg tatctcattg gataacttga ttggaccatt cttgtggtat
    20521 tctcttctat tcatgatctt tggcattttg attttttctg ttttagcagc tttatgtggc
    20581 tctttggtca acaaagcaga agatacagca aaagcaattt tgccagtgac ttatctatct
    20641 ctaggcggat acatgcttgg attgatacta ggagcttcag atccaaacaa tatcgttatt
    20701 cggatcactt cgtatattcc attcttatct tcatatatta tgccggttcg cttagcaaat
    20761 gagacagtcg gtatgggtgg agcattgatt tccttgttgg tacttgtagc cgtaacattc
    20821 ttattgatga tcttgtctgc aaacatgtac aaatcgaatg tactcgttta tagcgaaggc
    20881 ggattatgga gttcattgaa acaatcgatt tcaattatga ggaatgaacg aaaaaaagca
    20941 taaaacaatc ctaaaataca gaaggaagct ggacatctat tgttcccagc ttctttttgt
    21001 atagacctat tttatttatt ttactatatt ggatatatat tcattgaaaa tagtgtataa
    21061 caactttata atattgagta taccaatttt tggaggcgtt ttaatggacc gttatttaga
    21121 tttactttca gaaaaattcc cctcaacgga ggatgtcatc acggaaatca tcaatctcga
    21181 agcaattttg cagcttccaa aaggaacaga gttatttttg agtgatatac atggcgaatt
    21241 tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga
    21301 ccttttttct gaacaaatga cggaaacgga tatcagccaa ttcactattt ttacggctta
    21361 tccagaatat gcattaacga ctgattggta caaagaacaa gataaatcgc agctgatcca
    21421 tcatttgatc gatcttttac ggtttacgac agtcaaatat acccggtcaa aagttcgaaa
    21481 aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg atgaccgtat
    21541 caatggaaaa aaagactatg taaaaaaaat cattgaacaa cttgttttga tgaaggaaga
    21601 agaaaaattt ttaacgaaac ttgcacagac gatccaaaaa tctgtcatcg atcatttgca
    21661 tatcgttgga gatatttttg accgaggaac tcaagcagcc aaagtgatgg atcgaatcat
    21721 ggatttttct tctgtagata tccaatgggg aaatcatgat attttgtgga ttggtgcgta
    21781 ttgcggctct gaggcatgtt tactgacact tttacggatt gcagcacgct ataattattt
    21841 atttgaatta gagaaagaat atgggttgaa tttacgtcca ttgttttctt ttgctcatca
    21901 aacttatcag aagaatccag tatttgcgcc taaaaacagt aaaaatctat ccgaaagaga
    21961 gcaagaagaa ttagaaaaaa tccatcaggc actttctatc cttcaattca aatcagaaca
    22021 tcagttattg gatcgtcgac ctgaatttaa aatggacgat cgtaaattgc tggaaaaaat
    22081 cgactatgaa gcatcaacga ttgatttaga aggtcagctc tatgagttga aaggaacttg
    22141 ttttcaaacg atccagccag atgatccaaa aaaattcttg gaagaggaac aaaaagtgat
    22201 tgatagtttg atgtattcat tccagcactc tttgagaatg caaaaacata tgacgttttt
    22261 gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtgctgtttc atggctgtat
    22321 acctgtagac gagaagggac agctcttagc atttgaatta gaccaatctt atcgcggaaa
    22381 agaactgctg ggcttttttg aaaaacagat aactgaaagc gcaaaagatc tgactgcaaa
    22441 agaagattta gcaaccgatt tgatttggta tgcgtggagt gggccatttt cgcctttatt
    22501 cggcaagagt aaaatggcga cttttgaacg ttattttctc acagagcctc atacgcacgt
    22561 cgagaaaagc aatccttatt atcacttgcg agatgaagag tggttcagtg aaaaaatttt
    22621 agcagaattc gacacaaaag aagaagggtc agcaatcatt aatgggcaca cgccagtaaa
    22681 agtaaaaaaa ggggagtcac cgatcaaagc aaatggaaaa gtatttgtca ttgacggagg
    22741 tttatccaaa gcttatcaaa aaacgacagg aattgccggt tattcactat tatgcaattc
    22801 ttatggtttc caaatcgtga cacattatcc gtttttgcct attgaacaac aatttgcaaa
    22861 aaaatccgac caaacaaatg tgaaaaaagt aatcgaacag caactaccac gcaaactcaa
    22921 tcgtgataca acaaaaggta tggaactgca gcagcagatt acccagctca aaaggctgtt
    22981 ggactatcga aaggacgatt gagaagaatt ccagtgaaca ttgttccata gactggagac
    23041 tgagacaaaa gtcgtgtcgc agtctctttt tccggataaa cggtgttctt ctgccaactc
    23101 ctcggtcaca agtcacacta ttcaacaaac atgaaaagct gttttttgaa attgctcctt
    23161 gtgactcaga gttgaacggc aggctcacga cctcttggta taaacggtgt tcaaaagcca
    23221 gtttctcggt attaagccaa atttatgcaa aaatgtgaaa agccattttc acaaaatttt
    23281 tcttaatact cgaaactaaa cagctttttc acaaccccct cagaaagtct attaaaattc
    23341 attcacaaag aaatcgaata agcgctgttc agatggatgt acacggtggg taagttccgg
    23401 atgccattga acaccgagga ttttatgcat atcatctttg ctttgaaccg cttcaatgag
    23461 tccatcgtta gaaaaagcga tggcttttaa taaaggtgaa agatctttga ctgcctgatg
    23521 atggtaagaa ttcacaaaat atggatcggg gagaaggttg cttaacaaac tgtctgaaac
    23581 aatcttgact tcgtgagtcg taaattgcgg ggcagtgggc tgttgttcat gttttactga
    23641 ccactttgga tataatgaaa gatcttgata aagtgtacct tccaaaacga cattcaatag
    23701 ttggagtccc cggcagactg caaaaattgg ttttctttgt ttcaatgctt ccaagatcag
    23761 tgcctgttca aattggtctc gttgaatatt tgtttccgtt agctttggat gaggatcttc
    23821 catgtaaagt tgtggactga catcttgacc acctgcaaga ataagtttgt cgatttgtcc
    23881 aatcagttga ggagctgaat ctggagacat gactggcaga ataagaggca atccttgagc
    23941 atctaaaact gcatccacga atccttgcgg tgtatagctc acttgatttc cttggaaagt
    24001 atctgttgct cgaatcagtt gatttccggc aatgccgatg attggtttca tttgtattcc
    24061 ccctaaagta gacttattgt aacaaggatt taaaaaaaat taaactatgt aagacttgcc
    24121 cctatactta tctgttcttg ctgtgttact atctgtaaag agaaatttcg tgtgaaattc
    24181 atgacgatag ttgaaaatat tgcttaattt gttataataa aaagtaagca gaaataaaaa
    24241 ggttgggcgt aagcttcaag gatctagttc ttcaaggggt gagaaggaca ttttattgaa
    24301 ggggcctgcc tatactaagt tatctttaga ggtacatact ttcaatgaaa aaagaaagca
    24361 tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc
    24421 cgaaaagcag aagaagaaaa tgaaaagcgt gctcaaacac tgcggcagca gctgaagatc
    24481 tatgcaacag aaaaagagca ggaattggaa acgatccaaa caaactatcg acaacaacta
    24541 aaacagttgg tggaagaaaa agaacaattt gaaatacaag aattagaaaa agaaaaaatg
    24601 aaattgaaag cagaagcaga caaggtgcaa gcaactttga aagatcaata tcaaaagcat
    24661 cagagtcaag cgatcggtgc gatcattgaa agggtgaaag aaacttatgg ccgtcactaa
    24721 gatggaaaaa gtgaccctca tttcagataa aagaaatcag gagaggattt tacaagcagt
    24781 acaaggaatc caaaacgttg aaatccggga tctgtatcaa aatagaacaa acaaccagtg
    24841 ggtggagaag tattttccag ataccacgat tattgataaa gaagcaaaat taagcgcttt
    24901 aggtaatcgg ttgaaagaca tccaagaagc tatccaattc atacaacacc acggcgatag
    24961 ccgtcaaaag aaactccatt tgaaacgtca agaactttcc ttacaagatt tggaagccaa
    25021 ttattccgaa gaaaatttca taaataaatt gaaagaagtc cttgatttaa aagaaaagtg
    25081 ggaacaacta actgaagaac gtgatcgttt agaagaagag gaagaatggc ttcttcattg
    25141 gcaacagctg gatattgctc caagtatgta tgcaagtaag acgaccattt ttgagatggg
    25201 gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa ctgagtaagc ttgatgacgt
    25261 ttatatggaa gaaatagatt atacgccaaa acaagtttat ctttcgtatg ttgctttaaa
    25321 aaaatcagtt gctgttatag aagaaattgc tgggaaatac gggttcaata aagaaacgta
    25381 tccttatgaa caaaatccta aagaaaaact gcaagagacc aagcagcaat tatccattgt
    25441 tcacgagcag caaaagaaga taacgacagc gttaggtcag tgcagtggct atatccaaga
    25501 atttgaatgg gtagaagaag tcacacttgc cgttgcagaa cgagaaaaaa taaaagaacg
    25561 gtttatccat gcgacttatc tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca
    25621 gaatttgctg catgtgttaa acgaaaccgt atcagaagaa gatatatacg tcagtttcga
    25681 aacaccaaac tcagaagaga ttcagcagga agtccctact aaattgaaaa accatccgct
    25741 tgtcgaacct tttgaattgc tgactgaaat gtacagtctg ccaaaatatg aagaagtaga
    25801 tccaacacct tggatgacac cattttatct tgtcttcttc gggatgatgg tcgcagatgt
    25861 tggttatggt ttgctgatgc tcattgggac aatactagct caaaaactac ttgttctacc
    25921 acgcgggatg aaacgtttcg taaaattctt tgagattctc tcgataccat cgatcatctg
    25981 gggattgatc tacagttcat ttttcggaca gtcattacca aaagaactgt ttggcatccg
    26041 cttgcctttc ccaatcctat ctacgacgga tgacgtcaat acgattttga ttttatccgt
    26101 gattttcggt ctgatacaga ttttggttgg attatttgtg gcagcaaagg aaaatatcag
    26161 gagaaaagat tacttgagtg ctgtcagtga tggatttgcc tggcaaggga tcctaattgg
    26221 gatcgttatt gcgttggttg gtgccatgtt gataaaaaat acagcatttg tttaccttgg
    26281 cgctgggcta tcgatcattg ctgccttatg tatcttgatc gttccgatta tccaaacgcc
    26341 gtctaaagtc aaaggtgctg ccaaaggtgc gtataatctt tacggactga ccggctatat
    26401 cggtgattta gtcagttata cacgtttgat ggcattgggt atttcaggcg gaagtattgg
    26461 agcggcattc aatatgctag ttgctttcat gccgccagct gctcgattca gtgtagggat
    26521 ccttttgatc atcgctttac acgcactgaa tatgtttttg acactgctaa gtgcctatgt
    26581 ccatggcgca cgattgcagt atgtcgaatt ttttgggaag ttttatacag gaggcggacg
    26641 tgcctttgat ccgctgaaaa cggcggaaaa atacgtcaac atcaatcaca agaaaaaaaa
    26701 caataaataa ttggaggatt tttatataat gatggattac ttaattagtc aaaatggtgg
    26761 aatggtattt gcggtattag cgatggcaac agcgacgatt ttttcaggga tcggttcagc
    26821 taaaggtgtt ggaatgacag gtgaggcagc agcagcattg acaacaagtc aacccgaaaa
    26881 attcgggcag gctctgattc tgcaattgct tccaggtacg caaggtttgt atggattcgt
    26941 tattgccttt ttgattttta tcaacttagg aaatgacatg tcagttgtac aaggactgaa
    27001 ctttctaggt gcttctctgc cgattgcttt tacaggcttg ttttcaggta ttgcccaagg
    27061 aaaagtcgca gcggcaggta ttcaaatttt agcgaaaaaa cctgaacatg ctacaaaagg
    27121 aatcattttt gctgcgatgg ttgaaacata cgcgatttta ggtttcgtta tttccttcct
    27181 attagtgttg aatgcctaag cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc
    27241 atctctcaga tgaacgaagc agcagagcag gaaagagcgg cgttagaaca agaagagcga
    27301 atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaag ttgaaacaga acatcaaaaa
    27361 caaaaagaaa aacaaatcga attactcgaa aaaaaatacc gtcagctaag gaatcgccag
    27421 caagtagaag tacgacaaga aaatttgaat gcgaagcagg aatttttaag acgattgttt
    27481 gcagatgcgg taactgaaat ggaaaattgg gatgaatctg aacaaatcca gtttatcaaa
    27541 aatgcattgt attcattacc tttgacagga aaagtagcat tcattgctgg agaaaaatca
    27601 gcagcctatc tttctcagac gttgttggat gaatggaaca atgaacttcc ctttatgatg
    27661 gttttaagtg atgaaactgt tgctgatcaa gcgggttttc tgataaacga tcaaggagtt
    27721 cagtataatt ttcttttcag cagccttgta caagatatcc aaggaacgat gagttttgag
    27781 attgccaatc aattattcga ataaggaggg atcagtgtat ggaataccac gaactaaatc
    27841 ccttgattcg cggaagagag cttgaacttt tgtccaatga gacatttgaa cggatgattc
    27901 aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc cacgatttac agtccatata
    27961 tttatgacgg atatgaatat gacttcgaac agaatctttt aaaggagcag agccgtctat
    28021 ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt ttggatctat acgatgcgtt
    28081 atactttcca taatctgaag gttttgacaa aagctgagat gacaaatcaa aatctggacc
    28141 atttgtatat cgacgatgga ttttattcat tagaaacctt gaaggatgcc attcatacgc
    28201 aagtttcaaa tgaacttcct gaaagcatta tggacagtat acgagaagta cgtgattatt
    28261 ttgaagagtc taccattctt cagggaattg acgtgattta tgatcgacag tttttgacag
    28321 agcagcgacg gcttgccgat aagctaggat atcaagaatt gctgacagaa gtcattgctt
    28381 ttattgactt gaccaatatc acaacgatgg ctcgaggaat attgcagaaa agaacatatg
    28441 ggttcatgac gacggtactt tccagctcgg gaagtatcga aaaagaagta tttttgaact
    28501 ttgtagataa agatatggaa gattacacga attttctatt agagacagat tatgctgatt
    28561 ttctgcgccc tgtcatccgt gagggaaata ttgatctcgt aaagcttgaa cagttgaaag
    28621 atgattatct aagttcattg tatcaagaag cacaaaccca ggctttcgga ccactgccac
    28681 tactagcctt tttgaacgca aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg
    28741 gcaaaagaag ccatttcgaa tcagaaacaa tcagaaagcg ggtgagacaa gtctatgacg
    28801 cataagatcg gtgtagttgg cgacaaagat tctgttttgc cattcaaatt gtttggcttt
    28861 gatgttcgtt atggtacaac caagcaaacc gttcgtcagg caattgaaga aatggcaaag
    28921 aatgagtatg gcgtgattta tgtcacagag caatgtgcag cattgagccc agaaacgatc
    28981 gaacgttaca aggaacaaat gatacctgct atcgtattga ttcccaatca tcaaggatca
    29041 ttagggatag ggctggaaga aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc
    29101 ttatagtatg aaaaggagat agaaattttg caaattggaa aaatcataaa agtctccgga
    29161 cctcttgtaa tggctgagaa catgtctgat gccagtatcc aagacatgtg tctagtggga
    29221 gatctaggag ttatcggaga aattatcgag atgcgtggcg atgtggcgtc tattcaagta
    29281 tatgaagaga cttctggaat tggtccaggc gaaccggttc gctctactgg tgaagcttta
    29341 tctgttgaat taggaccagg gatcatttca caaatgtttg acggaatcca acggccatta
    29401 gataccttta tggaaatcac acaaagtaat ttcttagggc gcggagttca attgcccgca
    29461 ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag aagccggaga aacggtgagt
    29521 gctggagatg ttatcgggat cgtagaagaa acaaaagtta taaaacataa aatcatggtg
    29581 ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag gttcattcac tattgatgaa
    29641 acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg
    29701 ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc cggatgcacc catgatcact
    29761 ggacagcgag tgattgatac attcttccca gtgacaaaag gaggagcagc agctgtacct
    29821 ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg
    29881 gacatggttg tctatgtggg atgtggcgag cgtggaaatg agatgacgga tgttttaaat
    29941 gaattccctg aattgattga ccctaataca ggagaatcgt tgatggaacg tacggtattg
    30001 attgctaata catcaaacat gccagtagca gctcgagaag cttctatcta tacgggaatc
    30061 acaattgcgg aatatttccg tgacatgggt tatgacgttg ctatcatggc agactccact
    30121 tcacgatggg cagaagcact tcgtgagatg agcggacgtt tggaagaaat gcctggtgat
    30181 gaaggttatc cagcttatct tggttcacgg cttgcggaat actatgaacg atctggaaga
    30241 gtcattgcat taggatcaga aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc
    30301 ccgtctggag gggatatttc ggaaccagtc acacaaaata cattgcgggt cgtaaaagta
    30361 ttctggggtc ttgattccag tttagcccaa aaaagacatt ttccttcgat cgattggata
    30421 caaagctatt ctctttattc aacagaagta gggaagtata tggatcatat ccttcaagct
    30481 gactgggcat ccatggtcac cgagggcatg cgtattttgc aggaagaaca gcagctgaat
    30541 gaaatcgtgc gtttggtagg gattgattcc ctttcagata atgaccgtct gacattagaa
    30601 gtggcaaaat ccattcgtga agattatttg cagcaaaatg cttttgatga tgtcgataca
    30661 ttcacttcaa gagagaaaca attcaaaatg ttgaagatga tcttgacctt tggtgaagaa
    30721 ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga tcatggacgg taccgtatcg
    30781 attcgtgaaa ggatcggacg aagcaaatat atcccggaaa cagaattaga aaaactagat
    30841 caaattattg gtgaaatcaa gcaaacgatg caaacgatcg tttcagaagg aggcatgagc
    30901 aatgattaaa gaatatcgta cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa
    30961 agttgctggt gtcaaatatg aagaattaat cgaagtccgt atgcaaaatg gcgagattcg
    31021 tcgtggacaa gtattggaag tccaagaaga taaagcaatg gtccaaatat tcgaaggaac
    31081 aagtggtatc aatttacgtg actcttctgt acgattttta ggccatcctt tagaactagg
    31141 tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg ggacgaccga aagataacgg
    31201 accagagatc ctgcctgaaa agcatttgga tattaacgga gaagtaatca atccagtagc
    31261 tcgggattat ccagatgagt ttatccaaac aggtatctct gcaatcgatc atttgaatac
    31321 attagttcgt gggcaaaaat taccagtatt ttctggctct ggtcttcctc ataaagaatt
    31381 agctgctcag atcgcgcgac aagcaacggt actcgattca tcagatgatt tcgccgtagt
    31441 ctttgctgct attggaatca cttttgaaga agctgaattt ttcatggaag attttcgtca
    31501 aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga
    31561 acgtatcgct actcctcgta tggctttgac tgctgcagaa tatcttgctt atgaaaaggg
    31621 aatgcatgtt ttggttatca tgacagacat gaccaactat gcagaggcat tgcgagagat
    31681 ttctgcagca cggcgagaag ttccaggaag aagaggatat cctgggtatc tttacacgaa
    31741 cttagctact ttattcgaac gagcaggaag aatccgcgga ctaaaaggat cggttactca
    31801 aattccgatt ctaacaatgc cagaagacga taaaactcat ccaatccctg acttgactgg
    31861 ttatattaca gaaggacaga tcatcttgac tagagagctt tacaagagtg ggattcagcc
    31921 accaattgat gtactcccgt ctctttcgcg gttaaaagat aagggaactg gacccggaaa
    31981 gacaagagaa gaccatgcag ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa
    32041 acaggcaaaa gaattagccg tagttttagg tgaatctgct ttatctgatg tagacaagat
    32101 ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa
    32161 ccgttcgatt actgaaacac ttgatctagg gtgggaactg ctatcgatgt tgccaagaac
    32221 cgaattaaaa cgaatcaaag acgacatgct ggacaaatat ctgccagaag ggaagtgagt
    32281 tctgatggca cgattgaatg tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca
    32341 gttgacaact gccacaagag ggcataaatt attaaaagat aagcaagatg aactgatgcg
    32401 tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt caagaaatgg aagagtctat
    32461 gacagaagca atgtctgatt ttgtattggc aaatgcttcg atcacggaac ctttcatcga
    32521 agaattattt gttttgccgg cggaaaacgt tgatttgtct atcgtagaca aaaatatcat
    32581 gagtgtaaaa gtacctgtga tgaattttga atatgatgaa cagctgacag acacaccttt
    32641 ggaatacggg tatttgaatt ccagtgccga actggatcgg tcaatcgatc ggtttactac
    32701 cctgcttcca aaacttttgg aactgacaga aatcgaaaaa acttgtcaac tgatggcgag
    32761 tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa tatatgacga tccctcaatt
    32821 agaagaaacg atctattata ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac
    32881 aaggttgatc aaagtaaaaa atatgggtac cactgactaa gtggtatctt tttttaaaag
    32941 aggaaaacga tgattaaaca aagcaaacaa ataaagaaac gtttgccggc tgttcagctt
    33001 ctcgcattag gattttttct cctgatttta gttggcggcg gcctacttac cctaccattt
    33061 tttagtaata gtgggcatgg gacaaatttt atcgatgcgc ttttcactgc aacttctgct
    33121 gtctgtgtaa cgggattgac aacattgaat acagcagagc attggaacag tgctggtcaa
    33181 tttttgatca tgcttttgat tgagatcggc ggtcttggtt ttatgatggt tccgattcta
    33241 tgctttgcat tgataaagaa gaaagtcagt ctcagcacac gtattatatt aaaagaagcg
    33301 ctgaatctag aagaagtatc aggtgtgatg aagctcatgc tttacatcct aaaattggcc
    33361 tttgtgatcc aattgattgg tacggttgct ttatcttttg tatttgtgcc tgaatttggc
    33421 tggttgaaag gtatctggta cagtttgttc catgcaatct ctagtttttg taatgcaggg
    33481 tttgatttgt taggagacag tttagccaat caccagcaaa atgtttattt gatcatggtg
    33541 gtatctgctt tgatcatttc tggtgggtta ggcttcatcg tatggcgtga tttactggaa
    33601 tatcatcaga aaaagaagat gacgattcat tcaaaaatcg cattaaccgt tacaggtacc
    33661 cttttattag gcggattcgt tgtgttcttt ttcacagagc gcaatggggc aacgctagtg
    33721 gaagggaata tgtttcaacg attggcaaac acctttttta tgagtgtgac acctagaacg
    33781 gctggatatt attcagttga ttacctccat atgagccatg caggtctgat tttaacgatg
    33841 tttttgatgt acatcggcgg aacatctggg tcgacggccg gcggattgaa aacaactacg
    33901 ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag gacgtacgag agcggaagca
    33961 tttggccgta ccatccgaca aggcgcagtg ttgcgggcac ttactttatt ttttgttacg
    34021 ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga ctgaaacgat acctaaaact
    34081 tctggcatcg aatatattgc ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg
    34141 atgggattga cgccagactt gactgcaata gggaaattga tcattatagc gcttatgtac
    34201 attggacgag ttggtgtatt gactgtggtc ttctctctga tgacaaaagc acaaagaaca
    34261 caatcaaaat acaaatatcc agaagaaagt gtcatgattg gataatttca tcttctgaca
    34321 gaaacctagt gatttagtaa aataag
//
LOCUS       DACCXW010000014        33453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCXW010000014 DACCXW010000000
VERSION     DACCXW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.014"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..752)
                     /locus_tag="IVY71_000554"
     CDS             complement(<1..752)
                     /locus_tag="IVY71_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7984627.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIV"
     gene            923..1291
                     /locus_tag="IVY71_000555"
     CDS             923..1291
                     /locus_tag="IVY71_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7984628.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1461..1892
                     /gene="mraZ"
                     /locus_tag="IVY71_000556"
     CDS             1461..1892
                     /gene="mraZ"
                     /locus_tag="IVY71_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7984629.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1913..2872
                     /gene="rsmH"
                     /locus_tag="IVY71_000557"
     CDS             1913..2872
                     /gene="rsmH"
                     /locus_tag="IVY71_000557"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7984630.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2877..3278
                     /gene="ftsL"
                     /locus_tag="IVY71_000558"
     CDS             2877..3278
                     /gene="ftsL"
                     /locus_tag="IVY71_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7984631.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3279..5471
                     /locus_tag="IVY71_000559"
     CDS             3279..5471
                     /locus_tag="IVY71_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7984632.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5497..6459
                     /locus_tag="IVY71_000560"
     CDS             5497..6459
                     /locus_tag="IVY71_000560"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7984633.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6473..7834
                     /locus_tag="IVY71_000561"
     CDS             6473..7834
                     /locus_tag="IVY71_000561"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7984634.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            7860..8948
                     /gene="murG"
                     /locus_tag="IVY71_000562"
     CDS             7860..8948
                     /gene="murG"
                     /locus_tag="IVY71_000562"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7984635.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8963..10183
                     /locus_tag="IVY71_000563"
     CDS             8963..10183
                     /locus_tag="IVY71_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7984636.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10339..11664
                     /gene="ftsA"
                     /locus_tag="IVY71_000564"
     CDS             10339..11664
                     /gene="ftsA"
                     /locus_tag="IVY71_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7984637.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            11687..12928
                     /gene="ftsZ"
                     /locus_tag="IVY71_000565"
     CDS             11687..12928
                     /gene="ftsZ"
                     /locus_tag="IVY71_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7984638.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12945..13622
                     /locus_tag="IVY71_000566"
     CDS             12945..13622
                     /locus_tag="IVY71_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7984639.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            13636..14235
                     /locus_tag="IVY71_000567"
     CDS             13636..14235
                     /locus_tag="IVY71_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7984640.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14250..14537
                     /locus_tag="IVY71_000568"
     CDS             14250..14537
                     /locus_tag="IVY71_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7984641.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14586..15368
                     /locus_tag="IVY71_000569"
     CDS             14586..15368
                     /locus_tag="IVY71_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7984642.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15389..16096
                     /locus_tag="IVY71_000570"
     CDS             15389..16096
                     /locus_tag="IVY71_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7984643.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16358..19156
                     /gene="ileS"
                     /locus_tag="IVY71_000571"
     CDS             16358..19156
                     /gene="ileS"
                     /locus_tag="IVY71_000571"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7984644.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19205..20731)
                     /locus_tag="IVY71_000572"
     CDS             complement(19205..20731)
                     /locus_tag="IVY71_000572"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7984645.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(20746..21393)
                     /locus_tag="IVY71_000573"
     CDS             complement(20746..21393)
                     /locus_tag="IVY71_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7984646.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21577..21906)
                     /locus_tag="IVY71_000574"
     CDS             complement(21577..21906)
                     /locus_tag="IVY71_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7984647.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22083..22811
                     /gene="sfsA"
                     /locus_tag="IVY71_000575"
     CDS             22083..22811
                     /gene="sfsA"
                     /locus_tag="IVY71_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7984648.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            22827..23840
                     /locus_tag="IVY71_000576"
     CDS             22827..23840
                     /locus_tag="IVY71_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7984649.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            23840..25117
                     /locus_tag="IVY71_000577"
     CDS             23840..25117
                     /locus_tag="IVY71_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7984650.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25180..27882
                     /locus_tag="IVY71_000578"
     CDS             25180..27882
                     /locus_tag="IVY71_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7984651.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28034..28351
                     /locus_tag="IVY71_000579"
     CDS             28034..28351
                     /locus_tag="IVY71_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7984652.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28381..28701
                     /locus_tag="IVY71_000580"
     CDS             28381..28701
                     /locus_tag="IVY71_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7984653.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            28809..30269
                     /locus_tag="IVY71_000581"
     CDS             28809..30269
                     /locus_tag="IVY71_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7984654.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30337..30558
                     /locus_tag="IVY71_000582"
     CDS             30337..30558
                     /locus_tag="IVY71_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984655.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30589..30771
                     /locus_tag="IVY71_000583"
     CDS             30589..30771
                     /locus_tag="IVY71_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7984656.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            30771..31184
                     /locus_tag="IVY71_000584"
     CDS             30771..31184
                     /locus_tag="IVY71_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7984657.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31307..32488
                     /locus_tag="IVY71_000585"
     CDS             31307..32488
                     /locus_tag="IVY71_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7984658.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            32877..32950
                     /locus_tag="IVY71_000586"
     tRNA            32877..32950
                     /locus_tag="IVY71_000586"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32911..32913,aa:Arg,seq:cct)
     gene            complement(33019..>33453)
                     /locus_tag="IVY71_000587"
     CDS             complement(33019..>33453)
                     /locus_tag="IVY71_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7984659.1"
                     /translation="EWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIKNPH
                     NFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILSVS
                     KRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        11154 a         5702 c         7082 g         9515 t
ORIGIN      
        1 acgattgctg aaaaagtctg attcatttgt tctactagtt ttaattgaat gcgagaagta
       61 gtcattgcct gctctgtttc caccaataca tcacgtaaat gatttttcaa agcatgattt
      121 tcttggaaaa gtttattttg cgctagttct ttcaatgcat ctaaattagc agaagtcgct
      181 tctctgaaat aaacgaggct tttttggata tccatgattt gataaagttg tttgttttca
      241 gtggatactt gaagtttggc ttccaaagta tcgcattgag attgaagcgt agctaaataa
      301 ctgttgtaag agagtgtgac ctgccaaatg aattgaaaga agatattgag attaggcgaa
      361 atatcacttt ttggaaaggt ctggctaaag actttcttta aaaacagtgg ttcatgattc
      421 gtaattgtga taagcttctt tttgggcgtc aaaataatag agagcgggta tgtatccact
      481 tgaaaataac cgctaggact tgttttagca aaaggatatt gtaacaataa taacgctggc
      541 tttgtcaact ttgattgatt gaattcttct gtacgggaat tttctgcatc gtccaatatg
      601 gaagtgatgt aatctgacgg aatttcaaat tgatgtgtca gctgatgaat ctcttcttga
      661 ttaggacgtt ctacacaata ccagatcgtt tggtcagttt cctttccggt attacgcagt
      721 ttctcattat ctaatgtaaa atatttgatc atgattttcc tcctgttgcc tgtttctttt
      781 attataacgg atagggaatt ggaatacatg gaatgtgttt gtaagatata gaatataact
      841 gcataaattt aaataaaatg aaacagttgt gtaaagtata tacagtatac ttgaagcaac
      901 gataaaaaag gagtttacgt tcatgggcac attcacacca cttatcttgt tttggtatat
      961 ttacccggta atcgttttgt ttggttgcca atttcttgta aaattatttt cgttaaatcg
     1021 tcgttttaaa ataaaagccc ctgatttggc gattcctttt ttgtggggcg gtatgcatgc
     1081 attgtctaga aatacaggga caatttctat cttaccgttt ttctttattt ctgttttgct
     1141 aatgggaatt tgtattgctg ttttccaagc atattattat gaagaaatta tttatccgcg
     1201 ttatttcaag atgacttggc gtttggtttt tctgttgaca atcgtcttgt acgctgtctt
     1261 gatcattgtg aacatacttt cttacctatg aaaaaaatag aaaagctgta aaaagtcttt
     1321 taaaaggctt tttgcagctt gatttttaaa aaaatgttta tgaataaaaa aagttgttcc
     1381 cggatttccc agaaaaggtg gtagaaagtg ggggattgtg gtagactaat tttagttagt
     1441 ggaggaatgg ggggctttca atgttgatgg gtgaattcca acataatatt gatgcaaaag
     1501 gacgtttaat cgtgccctca aaacttcgtg aagaacttgg cgaaaagttt gttctgacac
     1561 gcggattgga tggctgtctt ttcggctatc cgatgagcga atgggaaaat ctggaagcta
     1621 aactgaacga aatgccgttg gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt
     1681 cggctgccac tgaatgcgaa cttgataaac aaggacgaat caatataccc agcacgttaa
     1741 gaaattatgc agctttgaca aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa
     1801 tttgggatga gacacgatgg caagattttt ctgcagcagc agaagagaac tttgacgaaa
     1861 ttgctgaaag tatgattgat ttcggactat aagaatagga agaggctgac taatgactga
     1921 aacatttcaa cactatacag tcatgttgaa agaaacggta gatggattga acatcaaacc
     1981 ggatggcgtt tatgtagact gtacactcgg cggtgccgga catagtgaat atttattaag
     2041 ccaactaagt tctaaagggc atctgtatgc gtttgaccaa gatcaaaaag caatcgatca
     2101 cgccaagctg cgtttagctt cttatgtcga acaaggtatg gtcacattta tcaaagcgaa
     2161 cttcagagaa ttagaagaac gcttgagtga gcatgtagat aaggtagacg gcattttgta
     2221 tgatctaggg gtttcctctc ctcagctaga tgaagcagaa cgtggattta gttatcatca
     2281 agatgcaccg ttggatatgc gcatggatca gtcagctcct ttatccgctt atcacgtaat
     2341 taatgaatat tcctaccatg aattagtaaa gattttcttc cgctatggcg aggagaaatt
     2401 ttccaaacaa attgctcgtg aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac
     2461 tggtgagctt gtcgaaatca ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg
     2521 gcatcctgca aaaagaattt ttcaagctgt gcggattgca gtaaatgacg aactcggtgc
     2581 cgaagaggcc tctttagaac aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt
     2641 aatcacattc cattcattgg aagacagaat tgtcaaaagt atgtttaaag aatatagtac
     2701 ggtacaagat ctaccaccgg gacttcctgt catacctgac gaattccaac cagaactgaa
     2761 agtcattact cgtaaaccaa tccttccttc agaggaagag ctaacagaaa acaatcgatc
     2821 acgcagtgca aagttacgta ttgcagaaaa aataagaatg aaagaggagt agaatgatgg
     2881 cagaattaaa gaaaatgcaa gattacgaat atgatttacc tgaattggtg gatcagccgg
     2941 aacaacaaat gcctgattct tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa
     3001 aacgattaaa gcagatttct gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta
     3061 ttgttgcttt atgtgtttta acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag
     3121 aaatcacgtc aatacaaaca gaaatcaatg ataaaaagca agaaaaaacg agtttagaac
     3181 aagaaaaaaa cgaactttct cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat
     3241 tgtctattaa tgacgacaat ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc
     3301 ggcggtttat ggaaaagaaa aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca
     3361 ttttatttgc tacgagtatt ggattgttct ttttatttgc cgtccgtttc tcgtatatcg
     3421 ttattggagg tcatgttgct ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag
     3481 gaagtgaagt agtaaaagca aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg
     3541 cagaagatgc ctcctcttat tcaattaaag cgatactctc aaaaacatat acctctgggg
     3601 ataaaaaact gtatgttgaa gaaaagaatt ttgataaaat agcagaaatc ctgcataaga
     3661 atctttctat agacaaaaaa gatgcactga atattttaga ggacggcgca aaaaaagagc
     3721 tgtatcaagt tgaatttggt agctatggga aaaatatctc ccaagaaacc aagcaaaaca
     3781 tcgaagcgga catgaaaaaa gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc
     3841 ggatgtatcc aaatggcgtc ttttcttctc attttattgg atacgcagtt ccagacaaag
     3901 atgagaatgg cttagttggt aaactaggtt tggagtctgc ttacaatgat atcttgagcg
     3961 gtaaagatgg taagattatt tatcaaaaag ataattttca aaatcctctg ccaggtacag
     4021 tggcagaaga agaaaaagct gtcgacggac aagatatcta cacgacttta gacagcagac
     4081 tacaaagtta tttagaaaca ctgatggatc aagtaaatga agaatatcag ccagaagaat
     4141 tgacagcagt cctgatgaaa gcaaaaaccg gagaaatctt ggcaatggga caacgaccta
     4201 ctttcaatcc tgaaacaatg gaaggattga ctggaaaaga tgctatctgg cgtaatttct
     4261 tggtacaaga tagttatgaa ccaggatcaa ccatgaaagt tttcactact gcagctgcta
     4321 tcgaagaagg agaattcaac gagaatgaaa ccttccaatc tggaaaaatc caagtagaag
     4381 atgctacgat caatgaccat gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc
     4441 tttcatggtc aagtaacgtc gggatggtca tactggaaca gcgattaggc ggacgatggt
     4501 ataactattt acaaaaactg ggatttggac aaagtactca ttctggatta gatgatgaag
     4561 taaacggtgc tttacctaca ttaaatattg tcgaccgagc aatgagtgct tatggacaag
     4621 cggtaggtgt gacgaatttc caaatgatga aagctttcac ttctattgcg aataacggta
     4681 caatgattca acctcgatat atcagcaaag tggtggatcc ccaaactgga gaagagcgaa
     4741 caactcaaac agaagttcta gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg
     4801 aatatatgag agatgttgtc gaaagcgaaa actacggtag tgcttatggt gtatatagtg
     4861 tacctggata caatgtatcg gcaaaaaacg gaacagccca aatcgcttct gatacagggg
     4921 gatatcaaac aggagataca gcctacctgt attcgattgt ggagatggtt ccttctgaag
     4981 atccagacta tgtcttgtat ctaacaatga aacaccctaa gacatatgat cgaatggcat
     5041 tggctaaaat cgcaaatcca ttgatgaaac gggcaatgga ttttaaagaa actgaagaag
     5101 attcagatac agaaacaaaa acagaaaaag tatccgtagc cgattatcgt aatcttgaag
     5161 cggacgttgc agcagctgat gctcaaaaaa gtggactgca acctgtagtg atcggtaatg
     5221 gtaaaaaagt acaaaaacaa tctactgcca acggtgatca gttgatctct ggagaaaaac
     5281 tgatcctata tactggtggt gataagctga tgcctgacgt aacaggatgg tcaaaagcag
     5341 atatcatgaa gctggggaaa atcctcggta ttgaagttag ttttgatgga gatggttatt
     5401 gtgtcaaaca agaacttgct ccatatgaaa aaatcactga agacaaactt agttttactt
     5461 tagaagaata agaagagaaa taggagagaa aagagcatgg aatggacaca agcattgatt
     5521 cccattgtca gcagttgtgc aatgacaatt gccgcaatgc cgctatttat tggttatttt
     5581 caaatgaaaa agcaaggtca ggcgattcgt gaggagggac caaagtggca caatagcaaa
     5641 gccggaactc ctacaatggg cggcttagtg tttctgatcg gctctatttt gacaggaatt
     5701 tgggttggag cttggcaaaa acagctaact cctactcttt tcatcttgct ttttgtatta
     5761 gcgttatacg gagtaatcgg ctttcttgat gattttataa aaatattcaa gaaaagaaac
     5821 atgggactga actcgaagca gaaattgatt ggacagattc tagggggaat catcttttat
     5881 cttgtttacc gttctgaagg ttatccaggt gtattgaatt tcttcggact agatctacct
     5941 ttaggaatca tttatggtat tttcgctctc ttctggcttg ttggattttc aaatgcagta
     6001 aatctgacag atggcatcga tggattagtt gctggcttag gaagtatctc ttttgcagct
     6061 tacggtctaa ttgcttggca tcagcatcag tatgatgtat taatcatttg cttaagtgtt
     6121 ttaggaggtt tactgggttt cttcgtctac aacagaaaac ctgccaagat ttttatggga
     6181 gatgtcggtt cattagcgtt gggcggactt ttagcggcaa tttctataat gctaaaccaa
     6241 gaatggacat tgcttttgat tggtttgatc tatgttatgg aaacagcaag tgtgatgctt
     6301 caagttactt cctttaagct tacaggaaaa cgaattttta aaatgtctcc aattcatcat
     6361 cactttgaaa tgtgcggatg gtcggagtgg aagattgata cagttttttg gttgatcagc
     6421 attgtgactt cgttgatcac attatggatc gtttggtagg agatgacggg aaatgaaaaa
     6481 aatatcaaca tatgaaaata agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag
     6541 tgcggccaaa ttattacatg aattgggcgc acttgtcacc gttaatgatg gaaaaccttt
     6601 tgacgaaaat ccggaagcac aagagctgct ttctttaggg ataaaagtaa taacaggtag
     6661 tcatccaatc gaattgttgg atgaagaatt ttcattgatg gtcaaaaatc cagggattcc
     6721 ctacagtcat cctttagtag caaaagccca agaaatgggt attccggtaa ttacggaagt
     6781 tgagctagct tacgaagtag cggaatgccc aattattggg attacaggaa cgaacggaaa
     6841 aacaacgaca acaacgatga caggactact actgaatgca ggagcagatc aaggaattgc
     6901 acgtttagcg ggtaatattg ggtatccagc aagtggtgta gcgcaagaag caaaatcaga
     6961 agataaaatt gtgatggaat tgtctagctt ccaactcatg ggaatcacag atttccgccc
     7021 tcatatagcg gtgattacca atatctatga agcacatatt gattatcatg gtacaagaaa
     7081 agaatatgtc aaagctaaat ggaaccttca aaaaaacatg acagaaaaag attatctgat
     7141 ccttaactgg aatcagtcag aactacaaga attagcgcaa agaacaaaag cacgcgtact
     7201 gcctttctcg acaaaagaag tgttggaaga cggtgtgtat gctgacgatt actcaatata
     7261 ctataaaaaa gaaaaaataa tggaaatatc tgaacttggc gtaccaggta agcataatgt
     7321 agaaaatgca ctagctgcta tttctgtagc aaaattgtat ggtatatcaa atgaagctat
     7381 acgagaaacg ttgcattttt tccatggcgt accacatcgt acgcaatatg taggagaaat
     7441 ccaaggcaga aaattttata atgattcaaa agcaaccaat attttagcga ctaaaatggc
     7501 tctaagtgga tttgaaacgt ctaaggtcgt gttattggct ggcggattag acagaggaaa
     7561 tacatttgat gaattaattc cttcattaaa aggaatcaaa gcaatggtcg tatttggcca
     7621 aacaaaagaa aagttgatgg atgctggaaa aaaagcaggc atcgagacaa tagtaacagc
     7681 cgattctgta gaacaagcag ttcctttagc attagaaaac agtacagatg gtgatgtagt
     7741 tttattatca ccagcgaatg ccagctggga tcaataccca aattttgaaa ctagaggaaa
     7801 cagattcatg gaagctgtga accgattaaa atagaaaaag tagaagcgag cgataaaaaa
     7861 tgaaaatttt ggttacaggc ggaggaacag ggggacatat ttatccagct cttgcctttg
     7921 tcaattatgt aaagactcaa gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag
     7981 gtctagaaaa caaaatcgtc cctgatacag gaatgccttt ccataccttg gagattcaag
     8041 gatttaaacg aaaaatttcc atgcataaca tcaagacggt ccgattgttt cttaagagta
     8101 tccgtcaagc aaaaaaaatc ctcagtgatt ttcaaccaga tatcgttatc ggtactggag
     8161 gatatgtttc cggagcggta gtgtacgctg cagccaaaat gggtattcca acagttatcc
     8221 atgaacaaaa cagtgtacca ggtgttacca ataaattttt aagccgatat gtggatcgga
     8281 tcgctctttc atttaaagat gcggcttcct tttttcctga aaacaaagca gttcttgtag
     8341 gtaatcctcg agcacaagaa gtagcagata ctaaaaaatc agatattttg aaaacatttg
     8401 atttggatcc agaaaagaaa acagtcttga tttttggcgg tagtcaagga gcacttaaaa
     8461 tcaatcaagc agtatcagtt tttttaagtg aatatattcc ttcttcttac caagttttgt
     8521 atgcttcagg agatagatat tatgaagaga tcctaaataa aataaatcct gaaatacaaa
     8581 atattagtgt ccagccttat atcaaaaata tggcagaggt catggcttcc agtgatctgc
     8641 tggtggggcg tgctggtgca acttcgatcg cagaattcac agctctgggg ttaccggcag
     8701 tgttggttcc cagcccatat gttacgaatg atcatcagac taaaaatgca atgagtcttg
     8761 ttcacgcagg agcagcaaaa atgattgcag ataatgaact aacaggtgaa tcactaagtc
     8821 agacagtcaa cgaaatcatg ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta
     8881 aagaacaagg aatccctgat gcttcaaaac gtctttatga cttagtaaaa caaataatca
     8941 aaaaataaag gcggtggtct tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc
     9001 gtcttctgat gaaaacaatt tgactccttg gcaaaaggct aaccgaaaat atctggcaga
     9061 acaagagatg aaaaaccaag agccaaaaaa agaagacata acattagata cacccctttc
     9121 agattctgaa gaagaaaaaa cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa
     9181 aaaaatcgaa ttttttgaag agatcaagca aaagaaaaac ggtggaccat ataacggttc
     9241 ttttttgaac cggctgccca atttaaaaag tcaacggcac aaagtactat ttcgtcggtt
     9301 agctttgatc atcactgtat tgggtatacc gctgattttt ttgatctatt atgtttctcc
     9361 ttatagcaaa ttacaagcag ttgcagtatc aggcaataag acagtcaaca gccaagaaat
     9421 catctctgat acaaaattgt cattaggaga aaatgtttgg ggacagtatt ttcatcgatc
     9481 tacttatatc gatcgcctta aaaaagcaca gccaagaatc gaaacagcaa atatacattt
     9541 caaagggatg aatgaattcg atttggatgt aacagaatat aaagagattg ctttgatcgc
     9601 aaaaaacaat caatactacc cagtgattga aaatggaaca gtattagatg aaaaagtagc
     9661 aaatcctaca aaaaatctcc ctatcttgga agagttcaaa gactctgcaa aaatcaaaga
     9721 attaacaaag caatacaatc agttatcatc agaattacaa aaagcaattt ctgaaatcaa
     9781 gtacacgcca agagcaagca ataaaaatct gatccaattg aatatgaatg acggcaatca
     9841 agttattgta aatatcaata atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa
     9901 agatatggat gaaaaaggtg tgatcgacat ggaagttggt atcttctcgc atccttacgg
     9961 tagcactcaa gaaacggaag aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa
    10021 tgcggatcaa acaaacgaat caagtgaaga ggcagaaacg caaaaagcac aaacacctgg
    10081 atcgtctagc acaaacggat catctgattc aaatgattca agtgaaacaa gtcaaataga
    10141 agataatcca agtacaactc cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa
    10201 gtaagaatgt actttctaaa aaccagtaat ttatggtaga attggaagca gtgaatatat
    10261 ctattaataa gtatatttta aaacggacgc tagttgttcg cttgtatatg gaatagtaag
    10321 taggagggaa tcccattcat ggcaaaaaca ggaatgtatg taggccttga tattggaacg
    10381 acatctgtta aagttgtcgt ggctgaatat atcgacagcc aaatgaatat tattggagta
    10441 ggaaacgcaa aatcagaagg gattaaccga ggcattatcg ttgatatcga taaaacggtc
    10501 caagcgatac aacgagcagt acgacaagca gaagaaaaag caggtatcca gatcaaaggc
    10561 gtaagtgtcg gtttgccagc aaacatgctt gaagtagaaa attgccaggg aatgattgca
    10621 gttaacggag attcaaagga aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc
    10681 cttgttcgtt caatacctcc tgaacgtcaa atcgtgtcaa tattgccaca agattttact
    10741 gtcgatgggt tcgagggaat caaagatcct cgtggaatga tcggcgtaag attagaaatg
    10801 tacggtctgt tgtttacagg acctaaaacg atcatccata acatacgtaa atgcgtggaa
    10861 aatgccggat taatcgtcaa cgaaatggtg attactcctt tagcgctgac tgaatcaatt
    10921 ctttctgacg gagaaaaaga ttttggtacg atcgtcattg atatgggtgg cggccaaaca
    10981 acaacggctg ttatgcatga taagcaattg aagtttacca atcttgacca agaaggtggc
    11041 gaatttgtca ctaaagatat ctctattgta ttaaatacgt catttaacaa tgcagaagcg
    11101 ttgaaaataa actacgggga tgcataccca gaacggacat caccagatga agaatttcca
    11161 gtagatgtga ttggtcagtc agaaccagtg aaagtcgatg aacgctactt gtcagaaatt
    11221 atttcagctc gaatggaaca gattttcaac aaagcaaaag atgcattgga ccaaatcgaa
    11281 gcattagagt tgccaggtgg cgtcgtattg acaggcggag ctgctagtct tcctggtgtt
    11341 gtggacttgg cacaagaaat cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg
    11401 gggctgagaa acccggtatt tacaaatgta ataagtatcg tggattattc agcaaacttg
    11461 agtgaagtgt atcaattggc aaaaattgct gttacgggtg aaacctcagc agctcgtcaa
    11521 atggtagttg aacaagaaac aacaaacaca tatgagagct atgaagcacc tgaagaaaca
    11581 atctacgatg agccggaacc aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt
    11641 ttctttacaa atatttttga ttaattcatc ctgggaggaa taagatatgg aattctcaat
    11701 tgataataat atcaatgacg gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg
    11761 aaatgctgta aaccgaatga ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa
    11821 cacagacgtg caagcattaa aaaattcaaa agctgaaaca gttatccaat taggccccaa
    11881 atatactcgt ggtttaggtg ctggttctca gccagaagtt ggacaaaaag cggcagaaga
    11941 aagtgaacaa tccttacgcg aagctttaga cggtgcagat atgattttca tcactgcagg
    12001 aatgggtgga ggaaccggta caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt
    12061 gggcgcattg actgttggtg tcgtgacacg tccattcact tttgaaggac cgaaacgtgg
    12121 ccgttttgct gcagaaggta tcgctcgttt gaaagaaaat gtggatacat tattaattat
    12181 ctcaaataac cgtttattag aagttgtaga taaaaagaca ccgatgcttg aagctttccg
    12241 tgaagcagat aatgttttac gacaaggtgt acaaggtatc tctgatttga tcacagcgcc
    12301 aggttacgtt aaccttgact ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc
    12361 attgatgggg atcggtgtgg ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc
    12421 aatctcctct ccattgcttg aaacatctat tgatggagct gaacaagtac ttcttaacat
    12481 cacaggcggt ttggatatga cattgttcga agcacaagat gcttcagata tcgtagcaaa
    12541 tgcagcaact ggcgatgtaa atatcattct aggtacatct atcaatgaag aaatgggcga
    12601 tgaaatccgt gtgacggtca tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc
    12661 tagccgacca gctagacaag cacaaatgca atcacctgct caaaagactg tattagatat
    12721 ggatcaagct aaaccaactt cttcagaaga agaaaacagt tttggcgatt gggatatcag
    12781 acgtgaacaa aatgttcgtc ctagagttga tgattccaat tttgacaata tcgaaaaaaa
    12841 agaatttgat acattcaacc gcgaagaaac aaaatctcat ggcgatgatg aattaaatac
    12901 gccaccgttt ttccgtcgta aaaaataaag gagaggaaga gcacatgatt gctgacaact
    12961 tgcagaaaat caagcaagaa attcaggatt cgtgtgctct tgttcatcgt gatccgaaag
    13021 aagttacatt agtagctgta acaaaatctg ttgaatccgc tcaagctgaa gaattggtta
    13081 gagctggcgt tatgaattgt gcagaaaacc gagtagataa attattagaa aagaaacaag
    13141 atttatttga ttttcctgaa ataaagtggc atttaattgg taatttacag cgcagaaagg
    13201 taaaattgat tgtaaatgaa attgattact ttcatgcgtt ggacagttta agattagcag
    13261 aagaaatcca gaagcggtta gagaaagaat tatcctgttt tattgaagta aatgtttctg
    13321 gtgaggaatc aaaacatgga atttcaccag atgagctttt accgtttgtg gaatctttag
    13381 ctgcctgcac aaaaatcaag gttgttgggt tgatgactat ggcgcctaaa gaagcttctg
    13441 atgagcaaat ccataaagtg tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa
    13501 aaggttatgc ccatgcacca tgtagtgaat taagtatggg gatgagtcaa gactttcaaa
    13561 ttgcgattga agaaggtgca acatttatcc gtgtaggaac agcgctattc aaagacgaat
    13621 aaaggaggaa caaaaatgcc gcttttaaac agaggagcaa ttgctaattt tttcggatta
    13681 gctgatgagg aagagtacga ataccaagat tatcctgaaa caacaaaaaa ggctgtacag
    13741 ccaactaaaa aaaatacaac acagaatcaa tcaactcatt ctgtatctgc tgcgaatact
    13801 catgaaaaac aaaaaacggc gacttcacgt cctgtaacgc atgaaagtat tcagccggct
    13861 gaaaagaaaa aagtaattga actacatcaa tcacctacaa aaactgtagc gaaagaaaga
    13921 acagcttcaa gaacctctac acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg
    13981 gaaccaagag cttattctga agctatggcg attgctaaac gaatcattgc tggagaagca
    14041 accttagtga attttcattt agtagaagaa aaacaagcga gaagaatcgt tgattttcta
    14101 acaggaacag tttatgcact agatggagat atcaaacgtg ttggagatga gatttttctt
    14161 tgtacaccga ctgggatgga gattgacagt tcaaccgctc aatcttttgc agatgcgaat
    14221 ttgtttgatc tgtaagagga ggaactacga tttatactat tgcactatta cttcatttac
    14281 ttcaacgggc agcctatttc tatagcatta tcttgatggt atatgcattg ctctcttggt
    14341 ttccaggcgg gtatcaatca gggctaggac gattattagc taagatctgc gaaccgtata
    14401 tcagtttgtt cgatcgtctg cctttgagga ttggaccctt ggatttcaca attttagggg
    14461 caatcctgat cttgaactta gccgtacaag gtttagcaca gatcgttata tggataactg
    14521 tccatttagt ttattgattg aagcaacaaa atctttattc tcagaataga taggagggga
    14581 aaattttgga tgcaaatgta taccagcact ttcgggcaga tgaacgtaca tttatcgatt
    14641 cggtaggcga ttggattgaa caggttcaag gacaatatgc accctatctg accgaatttc
    14701 ttgacccaag acaatcctat attttagaaa cactgattcg acaaagcagt gatttgcgtt
    14761 tcatgtttta tggcggatat gaacaggcag aaagaaaaag atgtttgatt ttccccgatt
    14821 attacgaacc tgtagaggaa gattttgaaa cagcattgta tgaaatccat tatcctagca
    14881 aatttgccag cctaagtcat gggaaaatcc ttggaacttt agtgggaacg gggatcaaac
    14941 gctcttactt tggcgatatc atctcggacg gtgatcgctg gcaagtattt attgcaaatg
    15001 aggtggatca ttttgttgct gcacaagtaa caaagattgg aaaggttgct gtacgcttgg
    15061 aaccaatcaa atacacagaa atgattatgc cgaaagatag ctggacacaa gaaagaggaa
    15121 cagttagttc catgcgacta gacagtgtga tctctgagat ttataatatt tccagacagc
    15181 ggtccaagca gctgatcgaa gctggtaaag taaaagtgaa ctggacagaa aatacaagac
    15241 ctgattttgt acttgaacta ttagatatta tttcagtcag aggttatggc agagtccaga
    15301 tccaagaact tgaggggaag acgaaaaaag aaaagtacag ggtactgttc ggtgtattac
    15361 gtaaataaaa taaaaaggtg gtttactcat ggctttaact ccattagata tccaaaataa
    15421 gacattcccg acaaaaatgc ggggatataa tcaagacgaa gtagatgatt tcttagattt
    15481 agttgttcgt gactatgagg aattgactca aagaaatcga gaattagaaa aagcagtcaa
    15541 acattctgaa gaaaaacttg agtacttcaa tgaattaaaa gatgctttga accaatcaat
    15601 catcgtagct caagatacgg ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt
    15661 gatcgttact tctgcacaaa acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc
    15721 acatcaatta acaactgatg cagaagaaaa agcaagaaaa atcttgacag atgcaacaga
    15781 aaaagcaaga cagcttgcga cagaaactga agatcttaag aaaaaaacac gagtattcca
    15841 tcaacgtatc agcttgatgc ttgagtctca attagaacaa gtgaaaagtc cggaatggga
    15901 tgaaatttta caaccattct ccagctacgt gactgacagc catgaagtga tcaaagaagt
    15961 tttagcaaaa cagcttgaca atgaaaatga tactgatgta aactcagaca caggagtcat
    16021 agaagcacct atgacggcat ttgatacaca ggctattgat atctcgatcg tgcctggtga
    16081 aaatgaaaac gagtagaaca gaaaagcact ttgttattct taaagcgagc cgatgagagt
    16141 ggaagttcgg taagacactg catggtgaaa gatcctctac ttgagacctg cttttccgaa
    16201 attagagtaa gaagcagcgg ctgacctcgt tattggtcac agagagtttg cactagatag
    16261 atgcaactaa atttgggtgg taccacggca tgttcgtccc ttgcggctga acatgctttt
    16321 ttgtgtccgg aaaacaaata aaggagtgta tggttgaatg aaaatgaaag aaaccttgca
    16381 attaggaaaa acaaaattcc ctatgcgtgg taatttgcca aaccgagaag gcgagtggca
    16441 aaaagaatgg gaagaaaatc gaatctacca aaaaagacaa gagttgaacg aaggaaaacc
    16501 tagctttgtt cttcatgatg gacctccata tgccaatggt aatatccatt taggacattc
    16561 attgaataaa atcagtaaag acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc
    16621 accatatgtt ccaggatggg atacacatgg attgccgatt gaacaagtat tggctaataa
    16681 aggcgtgaaa cgtaaagaaa tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc
    16741 actttcccaa gtagataagc aacgagcaga tttcaaacgc ctaggagttg caggagattg
    16801 ggaacatcct tatgttacat tagatccttc ttatgaagct gcacaagtac gcgtatttgg
    16861 taaaatggct gaaaaaggct atatttacaa aggattgaaa ccaatttact ggtctccgtc
    16921 aagtgagtct tctttagcgg aagctgaaat tgaatataaa gatgtaaaat ctccatctat
    16981 ttttgtagct ttcaatgtca aagacggcaa agatattctt gatgaagata cagcttttgt
    17041 catttggacg acaacaccat ggacattgcc agcaaaccaa ggtattgctg taaatccaaa
    17101 atttacgtat gtattggtgg aagccgatgg ccgtaagttt gtcgtagcga aagatttgct
    17161 agaaacagtc caatcagaaa tcggttggga aaatgtaaca gttctaaaag aatttgctgg
    17221 acaagaaatg gaatatatga ctgcaagtca tccattctat gaacgtgaat cacttgtgat
    17281 tttaggcgat catgttactt tagacgctgg tactggatta gtccatactg ctccaggaca
    17341 tggggaagat gactatatcg ctggtagcaa atataaacta tctgtagtgt cacctgtaga
    17401 ctcaaaagga ttgtttacag aagaagcacc tggatttgaa ggtatctttt atgacaaagc
    17461 aaatcctatg atcactgatc tattaaaaga aaaaggagct ttgctgaagc tagatttctt
    17521 tactcatagt tatcctcacg attggcggac gaaaaagcct gtgatctatc gggcaactcc
    17581 acaatggttt gcatctatcg ataaattccg tcagaatatt ttggatgaag tagaaaaagt
    17641 agagtgggtt atcccatggg gtaaaacacg tttgtataat atgatccgtg accgcggcga
    17701 ttgggtaatt tcccgtcagc gtgcatgggg agtaccactt ccgattttct atgctgaaaa
    17761 tggagaagca atcatcacac cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca
    17821 tggatcaatt atctggtttg aaaaagaagc gaaagaactt cttcctgaag gatttaccca
    17881 cccaggttct cctaatggcg aatttacaaa agaaaaagat atcatggatg tctggtttga
    17941 ttctggttct tctcatgaag ctgttttacg tcagcgacca gaattatcat tcccagcaga
    18001 tatgtattta gaaggatcag accaatatcg tggctggttc aactcaagta tcacgactag
    18061 tgtggctatc aatggtgtag caccatacaa atcagttatt tctcaaggat ttgctttaga
    18121 tggtgaaggc cgtaagatga gtaaatcttt aggaaacatc attgcaccag aaaaagtgat
    18181 caaacaattt ggtgcagata tccttcgctt atgggtatct agtgtagatt atgaagcaga
    18241 tgttcgtgtt tctatggata tcttaagtca agtggcagaa gtatatcgta aaatcagaaa
    18301 tacgatgcgt ttcttattag cgaatacaga agatttcgat cctaaaaaag atactgtttc
    18361 gtacaatgat ttacgttctg tagacaaata tatgaccgtt cgattgaacc aagtaatcaa
    18421 agaaatccga gaagaagggt acgacaaata taacttcatg catatttacc gtacagttat
    18481 gaacttcttg acggttgatc tatcgtcatt ctacctggat tttgctaaag atgtagttta
    18541 tattgaagca gaagatgatt atcaacgtcg ttgcatgcaa actgttttct atcaaacagc
    18601 tgtagctttg acaaaattat tgactcctat cattccgcat acagcagaag aaatctggtc
    18661 attcctgaaa gaagaagagg agtacgttca gttaagcgag tttcctggtt atgaagagtt
    18721 tgcaaaccaa gaagaattga tggatacctg gactgctttc atggacttcc gtgataatgt
    18781 cttaaaagca ttggaagaag caagaaacag caaattgatt ggtaaatctt tggaagcaaa
    18841 actaactgtt tatccaaaac aacaagttcg tgaaatgtta aaagctttgg atgcagatat
    18901 tgcacaatta ttgatcgttt caccagacta ctttgaagtt atggcagcag atgaacaagt
    18961 gccagataac gctatggtat ttgatgatgt tgctattaca gtggaaaaag cagacgggga
    19021 aacttgcgat cgctgtcgcc aagttcgtaa agatgtagga gtagatgaaa aacttcctca
    19081 tctatgcggt cgttgtgcaa aaatcgttga agacttctat cctgaagctg tagctgaagg
    19141 attcgaagaa aagtaaaagg gaaaaggggg tgtgacatgt tctttgtcac accccctttt
    19201 gttatcaatc gtttaatttt ccacgttcac gataccagtt atctggttgc cagatccatt
    19261 cgaatccgtc tttttcaagt agttcgaaag ctgctttagg tcccattgat ccagctttgt
    19321 agttgggaaa atccggttgt gtttggtccc aagcttttcg aatgacatcc acgatatgcc
    19381 aagattgagc gacttcttcc caatgagaga agttcgttcc atcaccattc aacgcatcta
    19441 atagtaattt ttcgtaagct tctgggctat tttcaaccat ttctgcacta tttctaaatt
    19501 ccaatttgat tggttccgtt tcaaatcctt gccctacttc tttcccattc aaggataaag
    19561 aaaatccttc agttggctga atatagatcg ttagaacatt aggtggaagt gtcgtatcgt
    19621 cacatggttc atcgacagat gttttgaata cattaacagg aacttgtttg aatacgatgt
    19681 tgatccgagt ccctttttca gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg
    19741 accaacggaa gttatcgata gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag
    19801 catccacttt gtcttcttcg cgatagccct tgaattgtac accatccaat tcaccttgag
    19861 catattgccc gcgaacgaag ttttctaaca cttcttcttc gttataaatg cggatagctt
    19921 tcaatgcttt gatcttttct gtacggattt ctttttcgga aaatgctact ggcggttcca
    19981 ttgcaagtaa tgctacaacc tgtaaaatat gattttgcac catgtcttta agtgcgccac
    20041 tatgatcgta gtagcccccg cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga
    20101 tattgtcgat atagcgattg ttccattgag cctcaaaaat attattggca aaacggatag
    20161 ctgagatgtt ttggatcatt tcttttccta aataatgatc aatcctgaaa atatcttgtt
    20221 ctgggaatac ttctctgatt tgctggttca attcaaaagc agactggtaa tcagaaccga
    20281 acggtttttc tatgatcaaa cgattgaagc cattttttgt cataatattt tgtgatttca
    20341 aatgagaaac gatcgttcca aaaaattgag gtgccatagc caaataatag acacggttac
    20401 cgtttaattg atagtgtcca tctaatcggt cagacaattc ttttaatgtc tgataatgcg
    20461 ttgaatcatt tacatcatgg gattgatagt aaaaatggct tgcaaaggta gttgcttctt
    20521 catctgaagg attcaatgca cggattgttt ctttgacaac ttcacggtag tgctcatcgc
    20581 tccagggacg gcgagcggtt ccgattacag caaaatgctc gcctaaattt ccttttctgt
    20641 aaaggcggaa caaagacggg tacaatttac gctgggctag gtcacctgta ccgccgaata
    20701 tcgtaaacaa tacatttttt tcattagaca tgtggataca ctcctttata ttttatcgac
    20761 aaaaatcgtg ctggccgcct taaagctgac ggggattttc tttctatcat tttggatagt
    20821 gatcggtcct tcataagctg cgatgtcaac gatctcatat tcttcgttga tattcaggtc
    20881 aatagtgact aagtaatcca acaattcttt ttcatcaagc actcgtgcga tacgcacttt
    20941 tgtaccgaca ggataggact ccaatgtttg tcggcgttct tcatggacaa cttcctcttt
    21001 tttaggaatc gatcctccat gaggacaatg ttcaggatga ttcaaatagt tttctaaatg
    21061 atctgcaaga tgttccgaag tcacatgttc caatacttca gcatcatcat ggacttcatt
    21121 ccagctatag tctaaatgtt caactaaaaa aacttcccat aagcgatgtt tgcgaatcag
    21181 actgctagct ttttgcagtc cgcttgcagt caattgtaca ccttgataag gggaatgttc
    21241 tacaagctct tctttcacaa gtttagaaat catttcactg acagatgcag ctgagacatc
    21301 tagactagat acgatttgtt tattgttgac tttcgttgta tctccaccta gttctaaaat
    21361 gattttcaaa taatcttctc gatttggtgt catattcgat catcctttca tggtaaaaaa
    21421 ccatcattag taagtgtaac aaaaaaaacg ttaattgact atttttctaa ataaaaaaca
    21481 aaggattttt agacgttttt acaacaaaaa acgaaagatg atagagaaga ctcattcttt
    21541 cgtttataaa ggatatatat atacattggg agggatctat ttttcagata acaacttata
    21601 tgaagtgtct gcagattcgt cataaagagt ttgttcggga taagaaaaga taagataaaa
    21661 accatactga tcacgtacga ctaaacgata ttcatctatt tctaccacag atgtgccaag
    21721 agactgaccg ttgatcaaaa acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg
    21781 gctatcacta aagaaccatg taccttgata ctttgagagt ccttctgact tttccgcttg
    21841 ttttttcttt ttcaagtaaa taaaacttga gagaacggta atgagtgaac caagaaaata
    21901 gatcattttt tttatcacca aatcactcct ttatgagaaa ataatcgcat agacatattc
    21961 tttcttcttt atttcattgt accgaataaa agctaaaatt tctctaagac tttcgactgg
    22021 tttttttact gtttttctac aaattatgat aggatacata gtaggaagaa aggtgacata
    22081 atttgaagta tcagcaagtg gaaatagctt tttttattga ccgcccgaat cggtttatag
    22141 cacattgtat gaaaaaagac ggagaaataa ttattgcgca tgtcaaaaat acaggtagag
    22201 gaaaagaggt cctgatacca ggcgctgaag tggctattgt atttgctcct ggacctaaac
    22261 ggaaaaccgc ctatgatctg attgctgtaa aaaagaaggg tgactggttt aatatcgata
    22321 gtcaattgcc taatcggctg gcaattgatg gtattttaga cggcacgatc cttttaccag
    22381 gattaactgg tccatttacg gtatacaagc gtgaagtaac ttatcttcac tccaaatttg
    22441 atatttattt agagacggtg gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac
    22501 ttgaaaataa agcgattggt gcttttcctg atgcgcccac actcagagga ttgaaacata
    22561 taggagaatt aacgtatgcc gcacaagatg gttatgccgc ttatgtcttg tttattgctc
    22621 agttcgaaca tcttcatctg gcaacgatcc atgaagaaat gcaaccagca ctagctgata
    22681 tggttcgaca tgcgcagcaa agcggagttc aaatcctcgc ctataattgt caagtatcgg
    22741 cagatgaggt aactgtcaag caggccattc catttgatgt agatatccct tttgaagatc
    22801 ctatacaata gaaagaaggt aaaataatga tcaacttagg tattatcgga acaaattgga
    22861 ttactcatca atttgtccaa gcagctttaa gcacaaaaaa atatgaattg acagcggtat
    22921 attctagaca tttggaaaaa gcacaggaat ttggttctca ttatgaaggg gatatcgaat
    22981 tcgcaacaga tttgaagact tttttcaata ttactcatat ggacaccatt tatattgctt
    23041 ctccgaatag tcttcacttt agtcaagcta agcaagcaat tttggcagga aaaaatgtga
    23101 tagttgaaaa gccagctttt tcgacgccaa aagaaatgga tgaaatcatc catttggcaa
    23161 atgaacacaa tgtgtatttt tttgaagcag caagaaatat ccatgaatca ggttttaaaa
    23221 agattgccga ttacttacca ttaaaagacg agataatcgg tgcaaatttc agctatatga
    23281 aatattcttc gcgctatgac caagtattag atggagaaga accgaatatc ttctcgactc
    23341 atttctcagg tggtgcattg atggatttag gtgtttacct tgtatatgca gctgttgctt
    23401 ggtttggaat gccaaaagaa gtccattatt tcccaagaaa gatttctact ggtgtagatg
    23461 gtcttggatg ggggatctta cgttatgaga attttgatgt atcgatccag ccaggtaaaa
    23521 tcggtgattc ttatttaccg tcagagattt attttgactc aggaacgcta gtgatgaatg
    23581 gagtcaatgc aattgaacaa gcacaatttc atgatcgcgc gcataaagaa atcattgatt
    23641 tggcggtaac agctgcagaa aatccaatgg cagatgaagc agaggatttt gccaatgtca
    23701 tggctcatcc gaaagatcca gcttggggat tgttgtatga agagtggatc gagctagcta
    23761 gaaatgtgaa ccaagtgatt tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag
    23821 ataatgagga agatttttaa tgaacgaatt tgtacaagta tttcccgaaa attggcagaa
    23881 aaaatatgag gataaagggt ttatctcacc ttctcttatt caacgagcag tttttcagcc
    23941 actaagtaat aaacaaagta tagtagcggt ttctcctaca ggttcaggta aaacactggc
    24001 ttatctttgg cctctactac taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt
    24061 tgcttcttct caagaactag ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga
    24121 caaagagttg aaagtccaat ctttggtggg cggagccaac gtcaaacgtc agattgaggg
    24181 tttgaaggaa aaaccagaaa tcttgattgg tacaccagga agaatattgg aattgatgaa
    24241 agcaaaaaaa ataaaagcac atcaagtgaa aacaatggta tttgatgaag cagaccagct
    24301 atttgacgct gggaacagcc aaatcattga tcaaattctt catcaagctc caacagagta
    24361 tcagctagct tttttttcag cgacagcaga tcgttccctt gaaagcattg aaaaaatcac
    24421 tgggaaaacg tttgagacaa ttgacgtgac agcagaggac gattctcgaa aaggactgag
    24481 acattacttt ttacgtgtac cgatacgaaa gaaagatgac tacttgagac gtctcacgca
    24541 tatagaagat tttcatggtc tgattttctt taatcaactg actgaattag gcgtaatgga
    24601 agaaaaattg ctgtatagag gtgtaccggt aggaagtctg gcctctgatc agaacaagct
    24661 actccgaaaa gcagcgctag aacaattcaa aaagaaaatg gtcagagcac ttttgaccac
    24721 agatgtggcg gctagaggtt tagatattac aggtcttcct tatgtagtga atgcagaagt
    24781 tccgttaagt gaagaagctt atttgcatcg ttcgggaaga actggtcgaa tgggtaatga
    24841 aggaatagta attacactcg tacaagataa taatctaaga gatttaaaac ggcttttacg
    24901 tccatcaaat ataacacttg aagagatttt tttatacgga ggtaagctgc aaactgagca
    24961 gccaaccaaa gaagatgcag ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg
    25021 aaaggagcag aacccagaat ataacaaaga aaaaataaga aaaaaagtta aaaataaatc
    25081 aaaaaaagag agaaataaag gagcaaggcg caaataagcg cctttttatg ctatgattat
    25141 gaaaacggga acaaaattgg ttgccagagg aggggttatg tgaaagaacg tcaagaagag
    25201 atcttgcagc tattgacaga aaaccaacaa ggattgacag caagtgatgt agctgagcgc
    25261 ttaactatcg atcgaagcaa tgcaagccga tatttaagtg aactatacaa ggcccatcat
    25321 attgtaaaaa ccgctggccg accagtcgtt tactctttgc caacggaaaa gtcaaaatct
    25381 gatgaagtgc atgtcgactc ttctacgcaa gtgacttttg aaacactcgt tggagaaaat
    25441 gattctttaa aagtcagcat acagcaggca aaagctgcga ttctttatcc accgcggggg
    25501 ttgcatacga ttatttttgg agaaactggt acagggaagt cgatgtttgc tgaatgtatg
    25561 tatcattttg caatcgattc agagatgctt tctgcagatg cgccatttgt ttctttcaat
    25621 tgtgcggatt atgctcaaaa tccgcaactg ctctttggac acatctttgg gatcaaaaaa
    25681 ggggcttaca caggagcagc tcaagatagt cccggtctaa tagccaaagc agacggcggt
    25741 atattgtttt tagatgagat ccaccgttta ccgccggaag ggcaggaaat gctgtttact
    25801 ttcattgata aaggtattta ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct
    25861 gttcagatca ttggggcgac gactgaatct tcagaaagtt tcttatctac gttcaatcga
    25921 cggattccta tggcaatcac cttgccgaat cttgcttcac gttcactgga tgagcgttat
    25981 gaaatcatct ctctttttat caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc
    26041 gaaaaagaag cgattcttgc cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg
    26101 aaacgagact tgaaactagt ctgtgcaaaa tcttttttac attaccgtac tcatcatgag
    26161 aaaaagctaa tcattcgaaa agaagaactt tcccttcaag tacaaaaagg attactgaaa
    26221 atcaaggaaa taccggaacg tctcgaccga ttcatggaca gcaaaagtca gtatttgaca
    26281 tttgaaccag gatttgctga tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc
    26341 tataacgata ttgaggaaaa agtcacttct ctttccgaaa ccggtgtgga gaacattgat
    26401 ctagagaccc tcatttcaaa agatgtggat gcttatttcc agacatatgt caaagaattg
    26461 actaaaagtt cgattcccaa agatcttttg ccagatgaaa tctggcggtt aaccaatcaa
    26521 ttgtatgatg tggctgaaaa acggttagac cgtatttata atgaaaaagc tcgttttgct
    26581 tttgctttgc atctgcaaag tacattggat cgtgtgagag aaggtcatat gatcgtccac
    26641 ccagatttga ataacgtacg gaaaaatttt aagagggaat tccaggtagc aatcgatctt
    26701 tcaacgatca ttgaagaaga agaacaagtt gagattcctt ttgatgaaat cggctttatt
    26761 agtatgtttc tttctatcaa tctaggagaa agtgagcctg ttaaagaaaa caaagtagat
    26821 gttctcatat tgatgcacgg cagacagact gcttcaagca tgttggagat ggcacaagaa
    26881 ctattaggaa caaaagtagg gaaagctttt aatatgccac tggttatgga agttcaaaca
    26941 atgtatgaag aagtgttaca atatgtcaaa aactatcaag agcatttgac gaatggccta
    27001 ctcttgttaa cagatatggg atcattaaac accttcagtg atttgattta tgaagagact
    27061 ggcattcgga cgaaagctat cacgatgaca agtaccatga ttgtgataga agcgatacgg
    27121 atggcaagtg ttggaagaag cttagaagat atttatcaaa atatccagct ttcctttgaa
    27181 tcgatcgtac gtgatcagtt caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc
    27241 gtaatcgtga catgctttac tggtgaagga gtagctgcca aattatatca gcgtattatg
    27301 ccagtgatta atcaaaataa ggtagaactg atccagatgc agtttttaga acgtgaatca
    27361 ttcaaaaaac acatcgatgc cttattggaa gaatatgaga tcaaagcaat tgcgggaaca
    27421 gttgatgttg attatcaaaa tatcccattt ttttcagcct atgacgtttt tgatgatgaa
    27481 aaactaaata ttttaaagcg tatagctgat gatgagatcc cgacagctaa aattgtcgat
    27541 tcacttgctg gatcattaag cgatgtcttt tctattgatg aactgatccg ttcattgcaa
    27601 aagacagttc agcagatcca gaccgatttg tcgattatcg tggaacctgg agtagatact
    27661 ggaatcgtta tccatttagc cttcatgtta gacggattga tcaaacaaga aagccatcgg
    27721 caatttgaga aactgtctat tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg
    27781 gcaaacttga tggcactaga acgaaaatat caggtaaaaa ttccagaaga tgatatcgca
    27841 tttcttacac aaatgtttct tgaaaataaa ataaaaaaat gaacacacta atcatactgt
    27901 ttacacacta aacatctgtg tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta
    27961 tttcagctct tttttttatt ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa
    28021 aggagtgtta aaaatggcaa aaaaaacaat catgttagtt tgttcagcag ggatgagtac
    28081 gagtttattg gtcacaaaaa tgcaaaaagc agcagaagaa aaagggatgg aagccgatat
    28141 ttttgcggtc tctgcctcag atgcagataa taatttagaa tctaagaatg tcgatgtatt
    28201 attattaggt cctcaagtac gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa
    28261 aggtattcca ttagatgtca tcaatatgca agactatggc atgatgaacg gagaaaaagt
    28321 tcttgcacaa gcagaaaatc taatgaaata aatcaagtat gcagttgggg gtataacaac
    28381 gtggaagatc aacaaaatct agaagccatc atggggttaa tcatgtttgg cggtaatgca
    28441 aagagcgatg caatggaagc gattgcggct gctaaaatcg gtgattttga gttagcggat
    28501 aggaaaatcg ctgatgcaga agaatcactt atacaagcgc atcatgcaca aacagacatg
    28561 ttgacacaag aagcacaagg aaatcatatg caagtgacat tattgactgt tcacagtcaa
    28621 gatcacttaa tgacatctat tgcgtttact gatttagcaa aagaaatcat cgaactttat
    28681 cgtcgcgtat acagcgaata aatacaactt cttttttgaa cggaagaaga aaaatttttg
    28741 aacggatttt ttattttgtt ataaggagaa ctctctttat ataaaaaact tataggagga
    28801 agtaaaaaat gaacggacta tcaagttgga tggagaaata catcctgccg gttgccgcaa
    28861 aaatcggagc gcaaaaacat ttagttgctt tgcgtgatgc ttttatcggc atactgcccg
    28921 caacaatggc gggatcagtc gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac
    28981 agtttaacag tacctatgct gacagtttag cagcagacaa aggaatcttt gccgtattga
    29041 atgtcatcat cggaatcaat ggttttgtct ggaacggtac actagcaatc gccggattga
    29101 tctttgcctt ttcttggggg tataatcttg cccgagctta tggagtaaat gatctagctg
    29161 gggggatcgt tggtttagct accttgattc aaggtatttc ctttggtcca gcattatcag
    29221 cagcattaga tgtgaatgtt ccttcaaatg tcgcagatgc aatcaatagt tctgagattg
    29281 gtgcaacgat tgcagatggc acactgtcag ttggattatg gggttggcta aaactagatc
    29341 atttgaatgg aaacgcttat tttacagtta tgatcatggg agctatttca gtcattattt
    29401 tttgtaaatt gatgcttgcc aacattacga tcaaaatgcc tgattcagta ccaccagcag
    29461 tatctaaagc atttgcagcc attttaccag cgacgatcgc tttatacgtc attgctatta
    29521 tcaattatat cgtaggtcgt gtagctgatg ggcaactgat cattgatttg gtgcaaaaat
    29581 atattgcaga accattccta ggattatctc aagggttggg agcagtattg atcgtcacag
    29641 tatttgttca agtattctgg ttctttggta ttcatggatc aaatgtattg gcaccagtgc
    29701 tagagggaat ctggggacaa gcacaactaa tcaatattga tattttccaa aaaggttata
    29761 acggaaaaac tggtacacca gccgtgttag atgcaatcga agatggaaaa gcttatatgt
    29821 gggtccgcgg atcgttcgat gcttttgcat ggtttggcgg atcaggcgga acgatcgtct
    29881 tactgatagc tatcttgatt ttctctaaac gtgcagatta tctaacagta gctaaattat
    29941 ccattgggcc tggtatcttc aatatcaacg aaccagtgat gtttggtctg ccaatcgtat
    30001 tgaatgcaat catgtttatc ccattcttga ttgctccagt agtagcaaca gcaattggat
    30061 ggacagctac ttatcttggc ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac
    30121 cgccgttctt attgtcattc ttagcaacag gagcggattg gcgggcaccg atcgtaacat
    30181 tagtatgtat ggtcgtgact ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa
    30241 tggaccctgc cgcaggcgaa caacaataag atgaacgtga ggagcgggac gctttaagtt
    30301 tcgttcctca taatgaattt tcgaggagga actgaaatga ttacaattgc cagtgcaatg
    30361 caacaagatg aaatcaaaga attgttagaa ggatacaatg aagaaggaat gacttttaca
    30421 tttaaagaaa aacaaggaat caagctgttc tttgaaacaa gtgcagaaga tccggaagtt
    30481 gcggctaaaa aagcaaagga actaatcaaa gcacagcctt ggggaacagt cctttatttt
    30541 caatcaacag ctgtttagaa aatggtttaa aaagatagga tggttgagat gataaaaaac
    30601 ggtttatttt tatgtagtat tggttctttg atcgtgttgt atagtttgaa tccacattcg
    30661 caaaggtatg atttgtatag tatggtcaca ggtctttttc taacaggatt aggaatctat
    30721 ttcttcatga aggggaaaaa aagagaagaa gcaaaggaga aaaataacta atggaaatca
    30781 cgaaaaaatt aaatattcca gcatcgtttt tttacgataa agttatggac tctgtccttt
    30841 tcgatgtacg taaagcgacc ggcaaatcag taactagaaa acagctgaaa aatttggagt
    30901 acgtcaaaca attctcgcaa aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa
    30961 accagtctta taagtttcgt acatcgacaa cgaagaatga attcgtcgtt cattatcaaa
    31021 tcaaaccatt ggacgattcg acctgtgagg ttcattacac agaaaaaatg gagtcttatg
    31081 gcatgcttca aaagttgaat gatatgcttt taggaacaat cttgatgtat tttaaacgga
    31141 gacgtttcaa aaaaatgcta gatatgatcg aagcaacgta ttaaatcaat gaaacatggt
    31201 actatttagt tattgaaaaa tgaagtaagc tagaaggaat ttttcatagg aagatagatc
    31261 tttcctgcga atcctcacta gcttttttgt aggaaggggc agcaaaatga caaacaaagt
    31321 aagagagcta ggtgtatctt cgattgagga aatgtttgat ttagccactt atgcatttaa
    31381 tggggcagat acacctgaaa ggcgagaacg ttttcgtaca cttgcggaga attcttggaa
    31441 ttacggattt tttgatgagg aggatcggct aacaagtcaa gtcatggcga ccccttttcc
    31501 agtgaatttt tacggacaag agtacctaat ggccggcatt ggctatgttg cttcttatcc
    31561 agaagcaagg ggacaaggcg ggatcaatca gatcatggaa aagatccttg aagattgccg
    31621 tgatcgtaaa gtaagcttat cttatctggc gccattctcc tatcccttct atcggcgcta
    31681 tggctatgaa caatcatttg ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt
    31741 tatcaaaaaa aaatctggaa aaataaagcg tatggatttt gaagatgcaa aagctgcaat
    31801 cagaagaatt tatgaacaga tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg
    31861 gtggtatacg tataaattca agcaaaaagg caattaccag tttgctttgc attttgatga
    31921 taatgaggaa gcaacgggat atatcgttta tcagcttgca acgtcttcct ttattattgc
    31981 ggagtgggga tttttggatc atgatgcttt ttgcagcctt gttcgcttcg taagttctca
    32041 caatggtgct tttgaaacat ttgaatattc atgcggctac ggcggtaatg atcaaaatta
    32101 tctattagaa aatccatttg cttctgtaat gatcacacct tatatgatgg cgaggatcgt
    32161 agatatttct ttgtttttgg aaaattatcc atttaaaaag agacaagctg catttgccct
    32221 acaaatagaa gaagatcatt atgcgaaatg gaatgaagga atcgtggaag taacaatcga
    32281 aaatggaaaa aatcatctct caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag
    32341 tatccaacat atcacgcagc tattattagg gtatcgtaaa gtggaagagt tggtattcca
    32401 agaaagaata caggtgacaa aagaatatga agaaacgtta agctcaatct tgccacagca
    32461 acagccaatt cttaatgact atttttgata aaaaacgggt gatgacaata tttttgtcac
    32521 cacccttttg tttttcattt ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc
    32581 gtaaatttga attttttgtt ttcttttgtt ttagggtttt cttttttgtg gtcttggatt
    32641 gcttctttcg taatcttttc ccgatttttt ttagatgaat ctaagcgttc tgcgtatact
    32701 ttttcttttg gcttatcatt cattggaaag gacctcccta cattatttca ttctacctct
    32761 agtttatcat ctgttaagga ctagtggaaa cgttttgcct aacttataag ctgatttttt
    32821 tagcagataa gcttgcaagt aaaaaacaaa caagctacaa ttgatttatt gaaattgttc
    32881 ctgtagctca gtaggataga gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca
    32941 gtcaggaacg tataaataac ccctgtatcc tttgcggtac aggggtttgt tttgtatttg
    33001 tgccaaaaat gtgccaaact agttatcatt tttatataaa ctatcgaaca cagtattgat
    33061 tttttcttta tcctgatctt caagctctcg aacaatatga agataagtag acattgtagt
    33121 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
    33181 taaaatagat gcatgtgtat gccttagtcc atgcaaagta aattgctttt caatccctaa
    33241 ttcttttaat tttttgcgca aatatttgct gacggcgttt gatgaaacta atccattttt
    33301 taagttgaaa aaaacaaaat tatgagggtt tttgatttca aaattctcga ataattcttt
    33361 ttgattgatt ttaaactttt ttaagagatc aatcgtatgt ttatcaatgg aaattttcct
    33421 atttgaagtt tcgttttttg tttttcccca ttc
//
LOCUS       DACCXW010000015        32820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCXW010000015 DACCXW010000000
VERSION     DACCXW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.015"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..2260
                     /locus_tag="IVY71_000588"
     CDS             <1..2260
                     /locus_tag="IVY71_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7984660.1"
                     /translation="AFRQKEFVAILGPSGSGKTTMLNVIGGLDNYDSGDMVINGKSTK
                     DFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIENVELGMTLSGVSKDEKRKRAEDALHR
                     VGLTNHMHKKPNQLSGGQMQRVAIARALANDPDILLCDEPTGALDTETSIQIMELIQE
                     LSKEKLVIMVTHNPELAHQYADRIIEFSDGKILTDSHPHIERPKDDQFNLRRTKMSFW
                     TALKLSFNNIRTKKGRTFLTSFASSIGIIGIAIVLSLSSGFQKQIDNTQAETMAKFPI
                     TISKVTTNQTRDDAGLGASKADYPDSKTITAKVSEEDKAQHTNKIDQTYVDYVTDIDP
                     NLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQNPDTESLSLSSTMSAMTGVGVSSFPT
                     QLDTSKENFLKDNYSLLAGSYPASATDVVLIVDGNNNTNINALKNLGFDVKEDEKLDF
                     DDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYDAMYQNASDELKISGILRVKSSSTMNL
                     LSPGIAYSDQLTTQIVNENKESEIVKAQRDSGVNVLTTEKVDENAKQTLLSYLGGDSL
                     PSSIMIYPNNFEDKEKILDYLDDYNKGKSDEDKIIYTDLAGTMTELTGGLMDAITYVL
                     IAFAGISLVTSMIMISIITYTSVIERTKEIGVLKALGARKKDITRVFDAETCILGISS
                     GILGVFIAWLATFPINSILYNMTDLKNVAQLNPVHAIILVIVSTILTMLGGHLPARMA
                     AKKDAAIALRAE"
     gene            complement(2375..2686)
                     /locus_tag="IVY71_000589"
     CDS             complement(2375..2686)
                     /locus_tag="IVY71_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984661.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            2867..4342
                     /locus_tag="IVY71_000590"
     CDS             2867..4342
                     /locus_tag="IVY71_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7984662.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            4389..4973
                     /locus_tag="IVY71_000591"
     CDS             4389..4973
                     /locus_tag="IVY71_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7984663.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            4975..5358
                     /locus_tag="IVY71_000592"
     CDS             4975..5358
                     /locus_tag="IVY71_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7984664.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(5675..5959)
                     /locus_tag="IVY71_000593"
     CDS             complement(5675..5959)
                     /locus_tag="IVY71_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7984665.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(6311..7021)
                     /locus_tag="IVY71_000594"
     CDS             complement(6311..7021)
                     /locus_tag="IVY71_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7984666.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(7018..8490)
                     /locus_tag="IVY71_000595"
     CDS             complement(7018..8490)
                     /locus_tag="IVY71_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7984667.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            8628..9101
                     /locus_tag="IVY71_000596"
     CDS             8628..9101
                     /locus_tag="IVY71_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7984668.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(9254..9805)
                     /locus_tag="IVY71_000597"
     CDS             complement(9254..9805)
                     /locus_tag="IVY71_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7984669.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            9910..10440
                     /locus_tag="IVY71_000598"
     CDS             9910..10440
                     /locus_tag="IVY71_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7984670.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(10923..11366)
                     /locus_tag="IVY71_000599"
     CDS             complement(10923..11366)
                     /locus_tag="IVY71_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7984671.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(11382..12542)
                     /locus_tag="IVY71_000600"
     CDS             complement(11382..12542)
                     /locus_tag="IVY71_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7984672.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13049..14749
                     /locus_tag="IVY71_000601"
     CDS             13049..14749
                     /locus_tag="IVY71_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984673.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(14851..15684)
                     /locus_tag="IVY71_000602"
     CDS             complement(14851..15684)
                     /locus_tag="IVY71_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7984674.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(15988..18153)
                     /locus_tag="IVY71_000603"
     CDS             complement(15988..18153)
                     /locus_tag="IVY71_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7984675.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            18413..20707
                     /locus_tag="IVY71_000604"
     CDS             18413..20707
                     /locus_tag="IVY71_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7984676.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            20700..21365
                     /gene="pgmB"
                     /locus_tag="IVY71_000605"
     CDS             20700..21365
                     /gene="pgmB"
                     /locus_tag="IVY71_000605"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7984677.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            21358..22365
                     /locus_tag="IVY71_000606"
     CDS             21358..22365
                     /locus_tag="IVY71_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7984678.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            22387..23406
                     /locus_tag="IVY71_000607"
     CDS             22387..23406
                     /locus_tag="IVY71_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7984679.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(23687..25054)
                     /gene="nhaC"
                     /locus_tag="IVY71_000608"
     CDS             complement(23687..25054)
                     /gene="nhaC"
                     /locus_tag="IVY71_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7984680.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(25163..26584)
                     /locus_tag="IVY71_000609"
     CDS             complement(25163..26584)
                     /locus_tag="IVY71_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7984681.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(26827..28704)
                     /gene="tdc"
                     /locus_tag="IVY71_000610"
     CDS             complement(26827..28704)
                     /gene="tdc"
                     /locus_tag="IVY71_000610"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7984682.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(28995..30251)
                     /locus_tag="IVY71_000611"
     CDS             complement(28995..30251)
                     /locus_tag="IVY71_000611"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7984683.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            30831..32132
                     /locus_tag="IVY71_000612"
     CDS             30831..32132
                     /locus_tag="IVY71_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7984684.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(32201..32800)
                     /locus_tag="IVY71_000613"
     CDS             complement(32201..32800)
                     /locus_tag="IVY71_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7984685.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        10074 a         6723 c         5958 g        10065 t
ORIGIN      
        1 cgctttcaga caaaaagaat tcgtagcgat tttaggtcct agtggttctg gtaaaacgac
       61 tatgctgaat gtgattggtg gactagataa ttatgattcc ggtgatatgg taatcaacgg
      121 aaagtctact aaagacttta aggacagtga ctgggatgcc taccgaaaca actctatcgg
      181 tttcattttt caaagttaca acttgattgg tcatctaggc attattgaaa acgttgaact
      241 tgggatgaca ttaagtggtg tttctaaaga cgaaaaacga aaacgggcag aagatgcatt
      301 gcaccgagtc ggcttgacaa atcatatgca taaaaagcca aatcagttat ctggtggaca
      361 aatgcagcga gtggccatcg ctcgagcact agccaatgac ccagatatcc ttttgtgcga
      421 tgaaccaacc ggagcgttag atacagaaac aagtatccag atcatggaat tgatccaaga
      481 gttatcgaaa gaaaaattag tgatcatggt cacacataat cctgaactgg cccatcaata
      541 cgcagatcga atcattgaat tttctgatgg taagatctta acagattccc atcctcacat
      601 cgaacgacca aaagatgatc aatttaactt acgcagaaca aagatgagtt tttggacagc
      661 gttaaagctt tccttcaaca atatccgaac aaaaaaagga cgtactttct tgacctcctt
      721 tgcttccagt attgggatca tcggaatcgc gatcgttctg tccctttctt ctggattcca
      781 aaaacaaatc gataacacgc aagctgaaac gatggcgaaa tttccgatta ctatttctaa
      841 ggtaacgact aaccagactc gtgacgatgc cggattgggt gcaagcaaag cggattatcc
      901 agacagcaaa acgattactg caaaagtcag cgaagaagat aaagcacagc acacgaataa
      961 gattgatcaa acatacgtcg attacgtaac agacatcgat cctaacttaa gtaataatat
     1021 cggatttacc cgtactacag gaatcaatct tttacgtgat gtaaatggca aagtacagcc
     1081 tgtcagcttc tcgaatcaaa atcctgatac ggaatctctt tctttatcta gtacgatgtc
     1141 agcaatgaca ggtgtgggag tgtcaagttt ccctacacag cttgacacta gcaaagaaaa
     1201 ctttttaaaa gacaattatt ctttacttgc cggaagttat ccagcctcag caacagatgt
     1261 cgtgctgatc gtagacggca ataataatac taatatcaac gcattgaaga atcttggatt
     1321 tgatgtgaaa gaagacgaaa aacttgattt tgatgatatc gttggtacaa cattcaaatt
     1381 agtaaacaac aatacttact atacaaaact tccaactggg aattttatcc ctaatacaga
     1441 ttatgatgcg atgtatcaga atgcttctga tgaattgaag atctcgggga tcctacgcgt
     1501 gaaatcttct tctacaatga atctcttatc tccaggaatc gcttatagcg atcaattaac
     1561 gactcaaatc gtcaacgaaa acaaagaatc agaaatcgtc aaagctcaaa gagacagtgg
     1621 tgtaaatgtc ttaactactg aaaaagttga cgaaaatgcc aaacaaacat tgcttagtta
     1681 tttaggtgga gacagcttgc cttcaagtat catgatttat ccgaacaatt ttgaagacaa
     1741 agaaaagatc ttggattatc tagatgatta caataaaggc aaatcagatg aagataaaat
     1801 catttatact gatttagctg gaacgatgac tgaactgact ggcggattga tggatgctat
     1861 cacttatgtc cttattgctt ttgcagggat ctctttagta acaagtatga tcatgatcag
     1921 tatcattact tatacatctg tgattgaacg gacaaaagaa atcggtgtac taaaagcttt
     1981 aggtgcacgt aaaaaagaca tcactcgcgt atttgacgca gaaacgtgta ttttaggtat
     2041 ttcttctggg atattaggtg tgtttattgc atggctagct acgttcccta tcaatagcat
     2101 cttatacaac atgacggatc taaaaaatgt tgctcaacta aatcctgtcc atgcgattat
     2161 tctagtgatc gtctctacta tattgacgat gcttggcggt catcttcctg cacgaatggc
     2221 agcgaaaaaa gacgccgcta tcgctttgcg tgcagaataa ggttgtgaca accacattta
     2281 gatttgagtc ataagagcgc tgaaatagta gaaaacagca agaaacgagg aagtaatatg
     2341 ttccttgttt cgcgctgttt tttttaatta ttttttataa ttctggtata cttttctttc
     2401 ctttgtacca caactcttct ggcgtatacc tgtcttttcc atttatgaat actgttccat
     2461 ctgcttcaat cgttacttca gtcccaatcc acatattacg agaaagtgcg agaagatttt
     2521 ttctttttcc tcttcctttt tttccaaatt gcaaggcatc ggttatctct tctgtccaat
     2581 gagatttcac gtattttact tctccgtttt caaatgtaac tttcaaaata ttcgctatat
     2641 tttccacttt tactgcttta ggaaatgact cctccatttt tttcactttc cttcctccaa
     2701 cactaagtat gcgctttctt ttagtgataa gtatatcata gtttattcta ttatcaaaaa
     2761 aacgcatcca ttccattgta gaatacttta ccgaacaagt tgaattgttt tcctcttttt
     2821 caccttttac tatagatagg taaagacaac taaggaggaa tctctcatga cagatcaacc
     2881 gtatgattta agaaaagtca tcgaagaact aaaaacactt ccaggtcaat atcacgaaac
     2941 agatattgaa atcgatcctg aagcagaatt atctggtgtt taccgttata tcggtgctgg
     3001 cggtacagta aaacgtccga ctcaagaagg accagctatg actttcaaca atatcaaagg
     3061 tttccccggc acacgtgtga acatcggaac aatggctagt cgtaaacgtg taggtgaaat
     3121 tttgcaccat gattatcgaa cacttggtcg tttcttgaaa gacgctgtag aaaatccaat
     3181 caaacctgtg aaagtagaca aagcagacgc tccagcacaa gaagtcgttc atctagctac
     3241 cgatgcagac ttcgatattc gcaacatttt gcctgcacct acaaatactc ctgaagatgc
     3301 tggtccatac atcacaatgg gtgtagtctt aggatcagat ccagaaaaaa caatgagcga
     3361 tgtaacgatc catcgtatgg ttctagaaga taaagatacg atcggtatgt acatcatgcc
     3421 tggcggacgc cacattggtc acttccaaaa acaattcgaa gccttgaata aaccaatgcc
     3481 tatcactatc aatattggtt tagatccagc aattgccatt ggcacgactt tcgaaccacc
     3541 taccactcca cttggctaca atgaactatg gtgtgcagga gctttgcgta acgaacctat
     3601 tcaaatcgtt gatggtatag cagtcgatga agtgggtatt gctcgtgctg aattcatcat
     3661 tgaagcagaa atcatgccaa atgaaacaat gcaagaagat atcaacacga actcaggaaa
     3721 agcaatgcca gaatttcctg gctatactgg ggatgctaat ccagctgtac aagtagtgaa
     3781 agtcaaagca gttactcacc gaaaagacaa tccaatcatg caaacaacga ttggtcccag
     3841 tgaagaacac gtatctatgg ccggtattcc tacagaagca agtattcttg ccttggtcga
     3901 taaagctatt ccaggaaaag tagtaaatgt ctacaatcct cctgcaggtg gtggtaaatt
     3961 gatgaccatc atgcaaatcc gtaaagaaaa cgaagcggat gaaggtattc agcgtcaagc
     4021 agctattctt gctttatcag ccttcaaaga attgaagaca gtcattttag tcgatgaaga
     4081 tgttgatatt ttcgacatga acgatgtagt ttggactttc aacactcgtt tccaaggaga
     4141 taaagatatc atcgtgatcc caggaatgag gaaccatcca ttggacccat cagaattacc
     4201 agaatataat cctggctcta ttcgtttccg tgggataagt gcaaaaacta tctttgacgg
     4261 tacagttcct ttcgatatga aagaacattt tgtacgtgca aaattccaag aagtagaaga
     4321 ttgggaaaaa tatttgaaat aatgaatgat ctgcaactgt tttgacatca taaaaaggag
     4381 tgcggaaaat ggaacaattg gcacaacgga agaaaaaaat cgtgattggt gtaagtggcg
     4441 cttcaggcac catttatgcc atcaacctag tgaagaaact aaaagaatat ccgatgatag
     4501 aaacacacgg aatcatcagt ccatgggcaa aacagaactt aaagctggaa tccgacttat
     4561 cactaactga attcgaaagc ctgtttgatt accattattc aaacaaagat ttaggtgctg
     4621 ctgtcgccag cggatcattt ttgaccgatg gaatgatcat cgtaccggca agtatgaaaa
     4681 cagtcgcagg tatctctgtt ggtttaggcg acaacctgat tgcacgtgct gctgatgtaa
     4741 caatgaagga gcaacgaaag ttagtcattg ttcctcgtga aacgccatta aatgccattc
     4801 acttggaaaa cttgactaaa ttagcacgac taggtgtgca aatcatacca ccaattcctg
     4861 ctttttataa tcagcctcaa acgatccaag atcttgttaa tcaccataca atgaaacttt
     4921 tggatcaatt tgaaattgta aatgagcatt caaaacgttg gcagggtgat taatatggaa
     4981 aaagaaacga tagttgaagt aaccaaacta ggtttcacga tccaagctgc tgttaaagta
     5041 atcggtaaag acttattgat cactttaaca ggcggagata caccacatat cggaacagtc
     5101 actacatttt ctcgtgatac agagatgcaa actgtacgtt tccctagcca cgatggtcgg
     5161 ttccataaag acgatgtctt agccgaaaaa atagctgtga tcatcaaaac tgccgttccg
     5221 ggaagttgta cgatcacttc cggtatccat gttgaccata tctccaatgc acaaatcaac
     5281 gcctcttttc cgatgtcaga agagttaggt tacaagattt tagattggtt ggaaaaaata
     5341 gatttccata tcaaataacc agtttattat aaagatggtg aaaaaccatt atgaaagaag
     5401 aggttgtgaa atcagcgtta tgacctgagt atgagagaaa aaaatggaaa aatagcttct
     5461 catattttgc cgtttttgag ctaataccga gggacagctg attgaacgcc gtttatcaag
     5521 aggttgtgat aaagccgctt cgtttcgagt agtaagaaaa attttgtaaa catgggtttc
     5581 cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca ccgtttatca
     5641 agaggttgtg acacgtcttt tgtcacaacc tcttttattt ttgcttcact gtaactgcta
     5701 caatatcacc gatttctttt ccgatgtttt tacgaatatc ttttcgtagt ccaaggatgt
     5761 agcagactga accatctgga tttttcacgc ccatattcac gatactgcct tcatacggca
     5821 cattgtcaaa agttgcttgg actttaactc ttccagcacc gaattctttt cttatatcat
     5881 acggaaaaat aatatatgcc ccgcccttgc cgacttcgga agcatagatt tttgattcaa
     5941 aagaataaat ttccctcatt ttatttctcc tattttttac atctagtata gcatagcaaa
     6001 aaaaagaagg aactgtgaca aaagtcttgt cgcagttcct tttttcgaat aaacggtgtt
     6061 ctcctgccaa ctcctcggtc acaagtcaca atagtcaaca aacatgagaa gctgtttttt
     6121 gaaattgctc cttgtgactc agagttaaac ggcaggctca caacctcttc cttaaacggt
     6181 gttcaaaagc ttgcatctac ggcactaagc ccaatcagat gaaaaatatg gagtgctatt
     6241 tttatctgat tgttcttact gcttgatgca gagcagcttt ttcacaacct cctacatggt
     6301 gttcaaaata ttattcgcct tttgcttctt tcactaattt tgcgaatgct tgttgttgga
     6361 attcagcagc taaggctttc gtacgttgtt cttgctctgc tttgctcatt tttggatttt
     6421 cagtcattgc ttttaccata ccgtcttgaa cataagcttt tgcttcactt tccatcgtac
     6481 tttgctgttt cttcactaat tcagctaact tggctgcttg gtccgtactt aattctaccc
     6541 atgtctctgg gattttgaat gtattcactc ggctgtcata ttcgtgattg ttatcagcaa
     6601 tattgaacca gaatttatac atgtcgtttt tgtattccca ataagtagtt ttcgtcactt
     6661 tttcagcatc gccgtctgtc ttttctactt tatttgtttc gttatccgta ccagttgctt
     6721 ttggtttttc ttgtttttca tttgttcggt aaagatacac tttttctttt ccattcccta
     6781 aagctttata caaaagcata tctgctcctt gggaatcagc actggaaaca attttttctg
     6841 tttttgtttc cgttactttt tccattccaa agtgattgga caaattagcg acgatcaaac
     6901 ttagtgaagc gacgaagatt agtccgaaaa tcaaagataa aaaggtttgc catgtttttt
     6961 tagcgaacac gttcgttaaa gcaaaagcaa gcaagctgac aatcaaaact ccaataatca
     7021 tgcattatcc cccgctttca ttagaacagg ttcttttttc ttcaataaaa aggcaataat
     7081 aaagccaaga atcccaaaga tgatcgctac gataaacgcg gcatggtaac ctgacagtgt
     7141 ggcattgatt gcctgatctt tataagctaa tggcgtatta tccaatacag atttgcttgg
     7201 gatatttgct tttgtcacat tcgtcaacac actgatcaaa atagctgtac caatcgagct
     7261 tgcgatttga cggaatgtat tatttaccgc tgtcccatga ctgatcaagt tatttggcaa
     7321 tgcattcata ccagaagtcg ttaccggcat catgaccatg gagatcccaa acatacggat
     7381 cgcataaagg atcacgatat ataaaactgg agtcgttttt gtcaagaaag caaatggtgc
     7441 agttgccgct gtcaaaatga acatccctgt gatggctaaa cgtttcgcgc cgtatttatc
     7501 gaaaatcgca cccgtcacag gcatcatagc ccccatgatc aatgcacccg gcaacaacat
     7561 caagccagaa tggaatgctg attctccacg aatattttga atatacaatg gtacgaccat
     7621 ttctgcccct accatcgcca tatttgttac accagctaaa acagctgcaa tcgtgaatgt
     7681 agatgatttg aatacacgaa gttccaagaa tggatgttcc aaatgtaatt gacgccatga
     7741 gaacaaggcg atgaaaatca caccaacaac taagaaacca attactttca agcttcccca
     7801 gccgtcatct cctacactag aaaatccgta aagtaaacta ccgaatccaa tcgttgataa
     7861 aatagcggat aaaatatcaa tgctaggatt tgacaatgga ataacacttt tcattaggaa
     7921 gaatgctaaa accaagacaa gaacaacgat tggcagtacc ataccaaaca agtcacgcca
     7981 atggtagtta tcaatgatat aaccagataa tgtagggcct aaagcaggag caagtccaat
     8041 aacgatccct gacatcccca ttgctgatcc tcttttttct ggcgggaaaa tagatagcat
     8101 gatattttgc aataatggca tcgatactcc cacaccgcaa gcttggatca aacgaccgat
     8161 tagtaatgta ctaaaattag gtgctgtaaa gcaagtaatc gttccgatca aaaagatcgt
     8221 catggcagaa agatataaat ttcttgaact gaatttattg atcagccatg ctgttattgg
     8281 gatcatgatc ccgttgacta gcaagaaacc agtcgttaac cattgcacat cagacgctga
     8341 aatatcgaaa gctttcatca atgtaggaaa agctgtcgtc aataaggtct gattcaaaac
     8401 ggtacaaaat gtaccaatta ataagacaac aactaaaagt gtccgattat atggttttcc
     8461 gtaaatatct acgggttggt tgttactcat atttctcctt ctttctgaat tttttctgaa
     8521 actactagaa tactttagtc ctaaattcgc tatttgtcaa cctacttgac acataagtta
     8581 atacggatat aattaaggga agcaagagga caaaaggagt gacaaaaatg gttggggctt
     8641 cccttaaacg tttgttggaa aatgatcaat tggttgttgg catcagtgaa ttaagtgata
     8701 tcgtaggtgt gtctccacgt caattaaggt attgggaaca aaagggattc attcgttcga
     8761 tcgaagctga tgcgaattgt cctagaaaat atcgtttacc tacagtcata aaagtagaac
     8821 tgatcaaaaa atatttagat gacggattta ctttgtcaaa agctgcagaa aaggcagaaa
     8881 tccgccaaaa gaaaatgcac catgtccgta aagttttttc aaaagcaatc aaagatatcg
     8941 aattgatcga cgaacgctat acgattttac aaatcggtaa cttcaaagaa gagaatgcta
     9001 agatgtacat cattcatgac aacgagacag acgaattaac ttacaagctc cttcctgtcg
     9061 atgaaaaagc tgactacgaa cagttgttaa aagaactcta aacgccacta atctcctaag
     9121 taccaagctt tttaataagt agatgctaca taaatacttg aaaaaaggcc tgtttgaaca
     9181 taagaatatc cgaacggtga tctgcgtttc tttcatatag aaaggaattc tccatcactg
     9241 ttcggatatt tttttatttt tttcggttac tttcttctaa tacatctgtt agccaatttg
     9301 catattctgt ttcccgattg atcgcatgcg ctaaaatcag gtagtgtccg taattttcat
     9361 gtttttcttt ttcagtagga aagatcgttt tttttcgctc taatagatga agtcgttttt
     9421 cttcatgaag gtcacgctgc tgctgaataa tttctactaa tgccggatca ttcacgtctt
     9481 tgacgaaata caatttcaga acaaattcgt ctcgattaac cggtagttca ttggtgggtg
     9541 tatgaatcca ctctgccaat tgttccttcc cttcttctgt taattcataa actttctttt
     9601 ccaatttttc acctgtgatc tctacttgat gctgaatgat tccttgctct tccatttttg
     9661 caagttcagg ataaatctga ctatgttttg cttgccaaaa ttctcctatt tcatttttga
     9721 acgattgatt caattcatat cctgtcatct tcttcttact taataaccct aacaaaatat
     9781 atcgaagtgt attttttctt gccatcagtt ttccctcctt gacaaatctt taaatccatt
     9841 ctatcataaa tgagtaatcg aaaacatgta gtttattaca tgttttaatt tataaggaga
     9901 tgcttaaaca tgaaagaatt taaagaattg gaagatttta ttggaactca ctttatctat
     9961 acgtatgaca atggttggga atatgaatgg tatgccaaaa atgaccatac tgttgattac
    10021 cgtatccatg gcggaatggt caaaggccgt tgggtaaaag accaagaagc tgatatcgtt
    10081 aaattgacgg acggcgtatt caaaatcact tggacagagc caacagggac agacgtggca
    10141 ttggacttta tgccaaatga aaagaaactt cacggaatga tctttttccc taaatgggtt
    10201 gaagaacatc cagaaatcac tgttactttc caaaacgaac atatcgcaga aatggaagca
    10261 gctcgtgaaa aatatgaaac ttatccaaaa ttacttgttc ctgaatttgc tacgatcact
    10321 tatatgggtg atgcaggtat agacaatgaa gatgttatta gcgaagaacc atacgatgga
    10381 atgccagatg atatccgtag cggaaaatac ttcgatgaaa attataaacg agtaaaataa
    10441 ggttgtgaaa gagaggttgt gaaatcagcg ttctgacctg agtattagag aaagaaacgg
    10501 aaaaatagtt cctcatgttt tgccgttttt tgagctaata ccgagggtca gctgattgaa
    10561 caccgtttat caagaggttg tgaaatcagc gttatgcttt gagtaataag aacgctagtt
    10621 agaaaatagc ttttcatatt ttctaggcta gtgggcttat taccgagaag cagccgtttg
    10681 aacaccgttt atcaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
    10741 tggaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt
    10801 gaacaccgtt tatcaaagat tgtaacaact gcatattcac acagttaaat tagtgacttc
    10861 aaaatgaaat aatgctccca gcaaaaaatt aggctgggag cattatttcg ttattctgtt
    10921 catcaaaaat cagcgttttg attccatttc atcacaagtt cctcctcatt tactgcttca
    10981 tctttttgct gtgtttgaat tgtaaaacct tgtgaaagat agaaatgaat agctgattga
    11041 tttttttgat agactgtcaa agtcagtgtt ttattggttt cttttatttt tttcattagt
    11101 tgcgtaccga ttctttgttg ccggtattcc cttttcacaa agattcctgc gataaactga
    11161 tcaatcaccc cagcaaaacc aataatttct tgatctattt ctgctaagta aatcactgca
    11221 tctggtaaag ctttcctaac agattctact tgattctccc aataagttga tggaatgaat
    11281 tcatgagaat ccaagttagc atgcagccaa atcgatagaa cagattccag ttctttggca
    11341 gacaattttg ttgttgtgcg gatcattttt tctctcctta cttattcatt gattttaaaa
    11401 taaaaaccat ttttttgaag ctcttttaca agttttcgcc cctcaaaaaa gtatatcttc
    11461 tcttgtttta cttttctaat accaaaagaa acagcaactt taggatcatc agctgaaacg
    11521 attcgataag ttccaccagg caattgatcc agcttgactt tcccatgatg gtcgctttgt
    11581 actgaaaacg gcacaccatt tctagtaagt gtttttttct tatgtttcaa aatgaatgaa
    11641 ctaccattta ccggtttccc tgacgaatca ctaataataa atgaaaaatc aagcactttt
    11701 ttacttaatc tatactgata gattagtcgt ccgataaaca ctaacagtat gactacagct
    11761 attatcgttc ctgcaagaat aaaccattgc cgcatggttc tttcctgatt tccttttcct
    11821 tcttcttttt ttaccgattc gttataagga atccgatgtc ctgtcactag caaacgatgc
    11881 gtgttgatca tatacggcgt acatgtaagc aaggtaacta agtcttctcc ttctgaaatc
    11941 gttagaacag acgtgtcatc tggcagtacc acttgtatat ttttaacttg ataagccaat
    12001 ttctccccca aaacagtcaa aataaacgtg tctccgtttt ctaaacgatc taattcggta
    12061 aataaccgtc gattaggtaa accgctgtga gcagagatca ctgaatgcgt cgaatttcca
    12121 ccaagcggga aagaggttcc ttgcaagacg cctgctccat tttcaaggat cctaccattg
    12181 agccgatcat aaagtgaaat atttaattgg atcttaggta tattgatact tccgatcagc
    12241 cattcctcta attggctgga tgttgtactt tcatttctgt tcttttcatt gaaaggatca
    12301 gtaccaggat tgaatccttg gtcagttaat cgttggtttt tctcctccat tttctttcgc
    12361 tgcacttcaa tttcagctgc ggtccgtttt tgtacttgtt cgatacgttt ttgatcaata
    12421 aaactattta aagaatccac ataaaatgga tataaagcga tcagtaaccc tacgacgata
    12481 catattaata aaaacaaacg aaacaaaagt cgacgagact gtttttgctt actctgcttc
    12541 acatgtgtcc tcccttgcaa gtttttttgt tctttctttt attatatggg ttcaggaaaa
    12601 aaatagaaaa tgaaagccga tgaggttgtg aaaaagctgt cctgcatcaa gtaataagta
    12661 cagcccaaaa aaatagctac tcatattttt tgttgggttg ggcttattac cgcagatgca
    12721 agcttttgaa caccgtttaa ggaagaggtt gtgaaaactg cgttatgctt tgagtaataa
    12781 gagcgctagt cagaaaatag cttctcatat tttctaggct agtgagctta ttaccgagaa
    12841 gcagctgatt gaacaccgtt taccgagagg tcatgaaaaa gctactttgt ttcgagtatt
    12901 aagaaaaatt ctgtttcaaa acaaggagat gcaacaaaac ttatgtcgca tcctctctcc
    12961 ttagaacttt cacttatgtt tttcttgtaa aattcataaa tattttcatg aattttctaa
    13021 tttcttgcct acctctcgca taggatatat gatgaattta tctaaagttt gcaaaggagt
    13081 tcgacaattg aaaaaatatg gcccattttt agcaattttt ttcattgctt tggcatttct
    13141 catgcctcat ttattcacac ataaactaat tttgggtgct gatgccccat tccattacaa
    13201 tcggttctat gatgccgcgc aacaaattaa atatggggat ttccattatt ttccttccct
    13261 ttatggattc cagcaaagtg gcaggatcgt gaatgccgtt tacggcccat ttttcgctta
    13321 tttacaagga ttgatcgtgt tgcttgcagg tagctggttt cattatcaga ttctttcaaa
    13381 tttcattctt tatcttattt caggattttc tatgctgatt ttactgagat atgtcaatgt
    13441 tcgaaattgg gtaagtgtac cagtagccgt tttattcatg agtacgtatt ctattcaata
    13501 ctggtctatc aaccaaggat tcactagctg ggggacttgt ttttatcctt tatgtatgat
    13561 ccctattgct gatttcgtcc ttcataaaaa gttccctgtt atcaaagtcg ctgtctcagt
    13621 gggacttatg acacagatcc atttactgtc ttcgatcatg ttagctttga tgtatatccc
    13681 tttttatctt tattactttt ttaaccagac tgaaaaagga aaagcactgc ttcaactgat
    13741 taagtcggtc ttgctttatt ttgttctgac agcaaatatt tgggcaggat tagctattgt
    13801 ttacagtggg aataagattc ttcctccctt tgttaacaga gaaatgagtc aaaaggcgat
    13861 agatctggat ggcagttatt ggctccatta ccctaaggcc tttcctatat tgatattatc
    13921 aggttttttg ctttttattt tctttcaaca aaagaacacc ttattccaaa aaatcgtgtt
    13981 gtacacaagt cttgttttct tttttctttc tactagcttg attccatgga cgacattgat
    14041 cgaaaaaaat gtgcctttta ttagtttgat ccagtttcca tttcgatttt tcgtgccatt
    14101 tacgctattg cttctacttt accttgccct ttcactgaac cagtggtctg aacaaaagtg
    14161 gttccgtctt ttaagtatat ttggcattct tattttttca gcacagacga tccagaactt
    14221 ataccagcat ctggaaaagt gggaaaatga aacatttgtc agtcgacata cttatctatt
    14281 cgatacgccg gaagaagcaa ggaaaacatt cttcagcaaa gatatgtcta ccagcttgtt
    14341 cgttttccaa aaaaccacac ctgactatct gccaatctat aaggagacaa aagacaataa
    14401 atacgaccgg tataacgaag aaatactaga aaacgaaaca acttttataa aaacacatcc
    14461 aggcggtctt ttgacaatca agtggaaaaa cactggggac aaaactgttc atatccctgt
    14521 tatcgtctac gatcggactg tgctacagca aaatggaaaa acattgactg attatgaagt
    14581 aacagatatc gggaccccaa tcgtgaagca gcaaaaagga atcaacgagc tcacgcttca
    14641 ttatcaaacg ccaatctatt tctatttcat tctttctctt actttgattg gctggtttac
    14701 cttgctatgt ttatttattt atcatagata caagctttta cgtgcttagg caaaagggat
    14761 aaatacatcc gatcaaaaaa ctctccaaac ggtataatcg tttggagagt tttattttaa
    14821 tcagtttttt tatgttatat gttatatttt ttattcgttc acatctaatt cgatttccac
    14881 cccgtagtga tcactgatga ttggactatc gatcccatca aaaatccgtt gatatttttt
    14941 aacctgcatt ccttcaggga cgaaaataaa atctatacgc aatttttctg tattttgttc
    15001 ccaaccatct attttctttt ccacagtagc tgcttttcct ttttcattcg cgacaacata
    15061 cgaatcttga agaagaaggc aactttctct tacagtataa taaccttgag agtctactgg
    15121 attattgaaa tcacctagga aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa
    15181 gtttttccac tcaaaataaa agccactttt tttatctgtc caccaagaaa agtggcagct
    15241 cgagacaaaa atgtcctgat catctatctt tgttttccca atcaagatct tgcgtgtctg
    15301 ataatcttct ttcttttgaa attgggagac ggtaaggacc tgactttcca atgttttttt
    15361 cgtaagcagt gcatttcctt cttcatattt atcataaccg atgtggctca tttcccaact
    15421 ccaaaaatac tcttttccgt gttcctttaa aagctgaact aaacggtagg caaaattatc
    15481 tttacggata ggtacttgat tctttaccgg acagaacttc attagcttcg caggttctac
    15541 ttcttcagat tccagtaatt ggttgatttc ttgtaaagca atcacttcat aatcttctaa
    15601 caggatctta tctgctaatt cttgcaattt tttctctggt tctggctcaa gccagctatg
    15661 cgtgttgagt gtcatgaatt tcattggttt tctccttgtc ttcatttcga aacaaaaggg
    15721 ttgtgacaaa atttttgtca caacctctcc agcaaatagt gttctcctgc taactcctca
    15781 gtaataagct caatggacca ggagctattt tttgtttggc tgtttttatc acttggtgca
    15841 gggcagcttt ttcacgacct ctccactaaa cagtattcaa actgctgctt ctcggtaata
    15901 agcccaacca aacgaaaaat aggaggagct atttttgttt ggctgttctt atgacttgat
    15961 gcaggacagc tttttcacaa cctctcctta cattttaatg gagccgatca ctttaccagc
    16021 tgtcactgta cctaattgct tgatcactac ttcttctact ttatcgtttg taaatacgat
    16081 catgatcgtt ggatcttttt cttcgttttg gatcacatca agatccatct cagctaattt
    16141 cgttcctgtt cctacttctt ggccttcttt gacagcaatc tcaaaagctg gctgattcat
    16201 ttggactgta tcgattccca tatggatcaa aacttcgata ccatctttcg tttccagccc
    16261 aattgcatgt ttgctaggga aaatacttgt cacttttcca gcgatcggcg caacaacttc
    16321 tcttgatgtt ggaataacag caaagccgtc acccatcatt ttttgtgaga ataccggatc
    16381 ttttacttca gataaaggaa tcacttctcc agaagctgcg gaaactagtt cttgttctat
    16441 acctaagtat tgcttactac ctgcttgtcc atttcctgta gaaaccattt ctggctctgg
    16501 aatctctgct cctgattgca gcagatcttc gatatctgat ttcaagacat ctgcttttgg
    16561 tccataaact gcttggactc cattgtcttt gacgatcaat cccatcgccc ctgctcgttt
    16621 ccaggcttct tcagtcccta ctttttctcg atcatttaca cttacgcgta accgagtcat
    16681 acaagcatcg acatctttga tattttcttt tcctcccagc aaatgaatga tctggaccac
    16741 ttgctgatct gctgttcctg ttccgctgtt agatacaacg gtttcttcac tgtcattgtc
    16801 gtaatttccg ttacgtcccg gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa
    16861 gaaatacgta acaacaccaa aagcgatcac acacaacacg aagttgataa gatctccacc
    16921 tagacccgct ttgatagcta acggtgtccg agtcagcaat tcgatattcc caaatgaatg
    16981 gacccgcaat ggcaagatat ctgccatcgc aaatgcagcc ccttggatca ctgaataaat
    17041 gacatataat ggcacagcag caaacataaa catgaactcc aatggttctg ttacacctgt
    17101 tagaaagaca gccaacgctg ctgaaaaata cattgattta tatcttgctt tcttatcagg
    17161 atcgacatta cgatacatcg caaaagccat tcccatcaag atacctgaag aaccaatcat
    17221 ttgtcctact ttgaaacgag ccggcgtcca gttatgcaac acaaactcat attgcgacat
    17281 atcccctgca cctttaagat tcactaaatc agtggcccaa gccaaccata acggatcttg
    17341 cccaaatact tgtgtccccg cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg
    17401 tgtatagttg ataggaatcg tcagcatatg atgcaatcca aatggcaata gtaaacgttc
    17461 taatgttcca aataaaaacg gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca
    17521 taaaccaaaa ttattgattc cagcctgtac gtttggccac accacagcaa gaatcaatgc
    17581 cacgattgtt gaccataaga taacaacaaa cggaacaaag cgctttccat tgaaaaatga
    17641 caaagcatcc ggcaattttc tataattata gtacttatta taagccattg ccccgacaaa
    17701 tccagcgatg atccctacga atactcccat attcaaagct ggtgcttcca atacacttgt
    17761 aaaaaatcct ttaaccatga tcttcgtacc gaacagagtg tgggtaaatg catcttcatt
    17821 cgcaagcatg tcacttgtga caccaaagat agccccagtg atccgattga ttaaaataaa
    17881 tgagatccca gctgcaaatg ctccgcctgc tctttctttt gcccaacttc ctcctattgc
    17941 taaagcaaat aaaatatgaa gattaccaat aattgcccaa ccaatatttt caattactcc
    18001 gcccgttgtg acaagcggcg tccaatcagg attgatcaag ggaattgatt tccctatact
    18061 gatcatcaac ccagctgccg gcataactgc taccacaacc attaaagcct ttccaaattt
    18121 ttgccaaaac tcaaaactaa atatcttttt cattccgttt agctccttta cgcaatcggt
    18181 ttcgttaaac gtattatagc gtgcgttttc ttttttgtaa atgctttatt ttttattttt
    18241 ctgtctatac cattctataa aacctataat acgtgtgttc ccttctataa ttttctccta
    18301 aaaatttatt tttttaagct tttttctatg aaatattttc ttttgtctat tgacttgtgc
    18361 aatcggttgc gtttattatg aacgtataaa acagctcatt aggaggagaa aaatgaaaca
    18421 gatcaaacga ctatttgcta ttgatccatg gaaaatcagc acgacacaat tagataaaaa
    18481 aaatcttcga ctgcaagaat ctttaacaag tattggaaac ggttatatgg ggatgcgcgg
    18541 gaactttgaa gaaacttact caggggacca ccatcaaggt acttatttgg cgggtgtctg
    18601 gtatcccgat aagacacgtg tcggctggtg gaaaaatggt tatccagaat actttggaaa
    18661 agtgatcaac gccttgaact ttatcgcgat agatatttcg atcaacgatc agctgatcga
    18721 cttggcttct ttggaaccag aagactttta ctgggaatta gatatgaaaa atggtgtatt
    18781 atctcgtaca ttcacaatca ctacttctac taataaagta cgcatttcct tcgaacgttt
    18841 tctgagcatt gtcaaaaaag aatcagctta tatctattta actatagaaa tgcttgaagg
    18901 tagcggaaca gtcaaactcg tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa
    18961 ctatgatgaa catttttgga atgagattga tcgtggaact atagaagaaa tcggctattt
    19021 gacgactaaa actattccaa acaactttgg gattgaacaa tttactgtta ctgcagcaat
    19081 gcgccatttc gttaatggaa aatcagtcat ccctgactat cacgaagata ctttagcatt
    19141 acatggttgc ttgtctgcag aattatctca aggtgagaca tggtcagtgg aaaaagaaat
    19201 tatcgttctt actagtagag atattccaga agcgcagcaa gcagaaaaag ctgtctcaga
    19261 attaacgaac cagacaaaca attatatcca agcaaaagaa gagcaaacag cggcttgggc
    19321 agaacgatgg aagcttgccg atgtggtcat cgaaggagac gaagaagcac aacaaggtat
    19381 tcgattcaat ttgttccaac tattctctac ttactacggc gaagatgaac gattgaatat
    19441 cggccccaaa ggattcacag gagaaaaata cggcggagcc acttactggg atacagaagc
    19501 gtatgctgtc cctctttatc ttgctttagc caaacctgaa gtaactaaga atctgctgaa
    19561 atatcggcat aatcaactgc cgcaagcaat ccacaacgct cagcaacaag gattaaaagg
    19621 cgcgctttat cctatggtca ccttcactgg tgtcgaatgc cataacgagt gggaaatcac
    19681 attcgaggag attcaccgaa atggcgcgat tgcctatgca atctacaatt atacgaatta
    19741 caccggcgat acggaatatt tgaaaaatga aggactggaa gttttagcag aaatcgcacg
    19801 cttctgggca gaccgtgtcc atttttcaaa acgagcaaat aaatacatga tccacggtgt
    19861 gaccggccca aacgaatatg agaacaatat caataacaat tggtatacaa atacgatcgc
    19921 agcttgggtc ttgcgttaca cgcgagaaaa ttatttaact tatcaaaaag aagctgctat
    19981 cactatcagc gaagaagaac tagccaaatg gaaagatatc gcagagaaca tgtacttccc
    20041 agttgacgaa gaattaggaa tctttgtcca gcatgacaca ttcttagata aagatctcat
    20101 gcctgtatcc gatattccca caagcgatct tcctctgaat cagcattggt cttgggacaa
    20161 aatccttaga tcttgcttca tcaagcaggc agatgtacta caggggattt atttcttcaa
    20221 tgatactttt acaaaagaag aaaagcaaaa aaactttgat ttctatgaac caatgactgt
    20281 ccatgagtct tccctttcgc caagtatcca ttcgatttta gcagcagaat taggaatgga
    20341 aagcaaagct gtagaaatgt accagcgaac agctcgcttg gatttggata attacaataa
    20401 cgacacagaa gacggcttgc acatcacttc catgactggt agctggttgt cgattgtcca
    20461 aggatttgct caaatgaaaa ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa
    20521 aacttggaaa gcttattctt tccatatcaa ttatcgcgga cgtttattgt tcatctcagt
    20581 cacagatgaa caagttactt tcgagttatt agaaggttct cctctagaaa tttacgtcta
    20641 tgaacagtct tattctttag cggatcgtct agaaataacg ctaagaaagg aggaagcaaa
    20701 tgtttaaagg ggtattgttt gatttagacg gtgtcattac cgatactgct gaattccatt
    20761 atcgtgcttg gcgaaagctt ggtcaggaaa tcggcattac gatcgaccac acattcaacg
    20821 aacagctaaa aggcgtcagc cgagaagact cactctcttt gcttttagct tatggcggaa
    20881 aagaacactc tttttcaaag gaagaatttg cagaattagc gaaaagaaaa aacgattatt
    20941 atttagagat gatccagacg atcgaaccaa aagatgtctt ccccggggtc gtgcctttac
    21001 tggattcttt aaaagaagaa aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc
    21061 cattcctact tgaaaaaatg ggtcttacac cttactttga tgctattgcc gacccggcag
    21121 aagcaacaaa cggaaaacct gcgccagata tcttcctcct tgcagcaaaa gctgtcggac
    21181 taaaagcaga agaatgtatc gggatcgaag acgcgcaagc cggtatccaa gccatcctct
    21241 caagtggcgc tcaaccaatt ggcgtgggac gtgctgaaga ccttggagag gagatcccgc
    21301 ttgtccctga tacgactttt cttacgatcg attacttgaa agaaaagtgg catgaccatg
    21361 gataatccag agatctccgt cgtaccagtt ccagagactg aaataaagaa aatcacattg
    21421 aagaacgact acttagaagt cgttcttctc aattacggtg cccgcctgca ccaaatcttc
    21481 gcaccagaca aagaaggaaa atcagaaaat gttttattgt cttacgatcg tttcgaagat
    21541 gttctaacag acaaaagctt ttttggagca actgtcggac ctgtagccgg aaggatccgg
    21601 gatgcttctt ggggtgagca tagattggag aaaaactgcg gatcacacca tatccacggt
    21661 ggcacgaaag gctggtcttt ccaattttgg gaagtagaag ttttcaaaac tccatcatca
    21721 ataggtgtgg tcttttactt aaaagatgaa ttttctacct atccaggacc aatcactgct
    21781 acgatcactt accgtttaac aaaaaacgaa ttagagatga caacctctgc tagtagcctt
    21841 gttgaaacca tctgcaatcc gaccaaccat gcctacttca acctttcagg aaatggaaca
    21901 agagacatct atgaacatca gttaacggtc tttttagatg gtgtgcttga attagatcaa
    21961 gaaaaactgc ctactggaaa ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa
    22021 tccccaacac tgcaagagat ccttgcatgt tatccagatg gactagatga tgtatttctt
    22081 ttgcatcatc ctaggctttc caaaacttcc ttacaattat tcgagaagca ttctggtcgc
    22141 caaatgacta tagcaacttc taataaaagt atggtattat tttctacgac tggattcgaa
    22201 gctgatttct ctgtcaatgg aaaacagatg cattcaaact acggattagc aatcgaaccg
    22261 caagaaatcc ctgatatcgt tcatttcccc aaatttggat cgatcaacct ccatcctggt
    22321 caagaacgga tcagtcagac gatttatcgg ttctctgcac aataacaaga gaaaggtgtt
    22381 tttatcatgg ctattaccgt aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc
    22441 gtctctcgtg tcatcaacga tcatcctagt atttccgaga caacaaagaa gaaagtccga
    22501 aaaattatgg atgaactagg ttatgttcct aacatgactg cccgcaatct aggaaaacgc
    22561 atttccagtg ccatcggcgt aatcttaccc cctcttgatt caaaagagcg gatcggtaac
    22621 ccattttatc ttgagatcat ggaagcaatc aacgaagaag cgcggaatta tgatatgact
    22681 acagcaatcg ctactgctaa aaattttgaa ttgctgttag agaacgtcca gcgtatgcat
    22741 ttacaaaaac aagtagatgg ttttattcta gtgtattcag atagtcaaga tactgtcatt
    22801 gattatttat accaaaacca catccctttc actttgatcg gacagcctta tcatcatgag
    22861 aacgaaatcg tttatgtaga taatgataac cagcttctag gtaaacaagc aacagaattt
    22921 ttgatccaga atggacataa gaatatcctg tttgtgacaa acacgactca tgaaagtttg
    22981 tatttcgaac gatatttcgg ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta
    23041 tccagtgtag tactagagca tccggaggat tatctgtcct ttgagaaaac gttgaaagaa
    23101 aacgaagcca cagcattggt ggtcattgac gatatcttcg cccttcgcac gatgcagttg
    23161 gcgcaaatgt atggctatgt cattccagag acactctccg tcattagctt caacaactcg
    23221 atcttctcaa cattaacaca cccttacctt acaagtatcg atatcgatat ctccgaatta
    23281 ggacgtgtcg ctatgcaaaa gctgcgcgaa ttgatccatg aagaattgag cagtggcgta
    23341 cgtttggtca tccctcatcg cttgatcaaa agagagacag tcgttccttt agaaaaccca
    23401 tactaaatag ctgactcact aaatacgctg accatttatc ctatggtcag ctttttatgt
    23461 aaaagaagat actacttcta acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt
    23521 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc
    23581 ttcggaaata agctgaaatt cacaaaaatt taaaaagcaa ttttcttgag tttcttctta
    23641 tttcttggag ttaaacgctt ctgtcccagc ctcttttttc cttccttcaa ttttttacac
    23701 gtcgacgatt caataaaaaa ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa
    23761 aggcgaatgg caagtaatgc aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc
    23821 tgactcccca cggtacgaga gaattgacgg tcgcacctga atccgctagt gttcgtgtca
    23881 aataggtttt tgataagcca acgtcatcaa aagaacgctt gaatgtttct cctggaagaa
    23941 tgatggacag atactgttct ccaacgatca gattgattcc gatacagctc aaggcagtaa
    24001 acccgactaa acgagaaggt cggtccatct tttcttttaa ttcttgaacg atggtatcaa
    24061 cgatcagata tttgatcagt aacccaccta atgccaatgc aagtaaaatc aaactagcag
    24121 aacctaacat actcatgatc ccgcctcgag aaagcagttc atctagcttt ggatcaggtg
    24181 tatttgcgac ataaccattc atcaagatgt ctgctgcttt tccgaccgtc aaacttttct
    24241 catggataaa agaaagaacg agagcagctg tcgatcccgc tagtaatgta gggatagccg
    24301 gaattttttt gattgccccc ccaaataaaa taactagtgg aatcaatgtc caaggagaga
    24361 tccaaaagct cgctttcatg ttttggatca tttcattgat actagctaga tcggctgaat
    24421 gactgttatg tcctaatatc gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg
    24481 tataacgcat accagcgata tgttcgaata gattaactcc tccaataccc gctgccaagt
    24541 tggtcgtatc tgataaaggc gagcagttat tccctaaaaa ggcaccggaa acgactgcgc
    24601 ctgtagtcat tgcatcattg aatccaagta aatgaccgat ccccaaaaaa gcaattccaa
    24661 tcgtcgaaat cgttgtaaat gaactgccga cagtcactcc gacaattgca caaacaagaa
    24721 atgctgtcgg taaaaagaaa cgcacagaaa caagtttaaa tccataaacc ataatcgtag
    24781 ggatggttcc agataaaatc catgaagcaa ctaagacacc aatcaaaaga aaaatcagga
    24841 ttggaatgat ccctggagtg attcctttga tgatcccgtc atggatctca tcccatgtga
    24901 actttttaaa tctgccataa aacaacaaac acatgaaagc tgcaaggatc gggatatgtg
    24961 gcgagagctg gaatccgatg atcagtactc ctaggagtgc caacaacacg atcaatacag
    25021 cgatactttc ttttaaagaa atatttttat tcatcgtcga tctcctattt caaatgaaaa
    25081 ggccctgcca tgaaaaaact attcacttgg caggacctcc tactaaatca gcgacaaaac
    25141 gtcggctgaa agacttattt ttttattttt cttctaaact tttttctacc tcaatccccc
    25201 aagaatcttt cttgaagtga ttgatgatca atggaataac gaacatgacc actgtgatag
    25261 ctgctactaa taatgcatca ctcaatgtcg ctgtacctaa tgtagcaaat acgacaacga
    25321 tgatagagaa gatcagcatg cagctgacta cccaagctaa tccgtttccg tttttaccaa
    25381 ttcggtaagg acgttcgaat tctggatgtt tcttacgcat tttaagcaat gaaacagcaa
    25441 tcaaaatgta cacgatacaa taaataacag ttgtagcatt tgtaagtgtc aagaatacag
    25501 cacttacgtc atccattaca ccataaagca aagcaaataa tgaaataacg attgattgtg
    25561 ttagaacgac atttctagat acgccgtatt tgttttcttt atggaatccg aattttggtg
    25621 gaaggaatcc ttcacgagct acttggatga ttgtttttga tggtccagta acccaagctg
    25681 ataattgtaa aagcacacca ataaatacca tgaagctgaa gatatttccg atcacttctg
    25741 gtaagcctag gatttgacag tcaatcaaga taggctgcgt gatatttgct aattccattt
    25801 ttccatctgg tacgacattt gctacaagca tcgcattgat gacattcaat aaaactaatc
    25861 cgatcaaagc gataaatacg ccttttgtat aattttttgt tgcgtctttc aaacgaggca
    25921 tgtaaacaga actcatttcg atcccaacaa aaataaatgt gataccagct aagtatttca
    25981 atgaatcaat atgttccaag tcagggaaag cttttgacca tgaaaagtca cctagatagc
    26041 cgtttgagac tagtccgact ttgaaggctg ccaaaacacc taaaacgaac atgatgacta
    26101 ctgggatata tacccctagc catacaccga tgttcccgcc gacttttgcc atatcgaatt
    26161 tcaagttcaa gatcgtgatg atccagtaga taactaaaat acaacctaaa acgaaccagt
    26221 gattattgcc aagttctaca ttaccaaatg tgtttcctaa caatggtcct aaagtagaag
    26281 cgaccattac catacctggg aacatttgca cccataatag ccatgcaacg acaaatcccc
    26341 atttgctgcc cagtgctgtt tttacccata actgaggacc gccttcttga ggaagcatcg
    26401 tggaaagctc accggaaacc attgatatcg gaccggcaaa aaacaaaact gcaaaaattg
    26461 aataaaaaat caatgtccaa ccgactgcgg ctaacgtagg aatactccga accgttgcac
    26521 acagtgccat tgtcagaccg ataaaggtcc caagacccat tttacttact tcagatttac
    26581 tcaccgttga cactctcctt gattttttgg ataaaataac ggtgtctttg aagtagtttc
    26641 agcaataaac gaaacaattc agcgaatatt tgaggggatt ctttgagttt attgcttgaa
    26701 gggtttcttc aaagacaacc tcaaaacaaa gggatcgaag agcttaggtc tccccttgct
    26761 cttcagatcc aaacatgcgt cagaaacagt tagtattcaa gctgttttct aacatgatta
    26821 ccttgcttat tttgcttcgc ttgccaataa ttggtctgcg tagatttttt ccaatttttc
    26881 ttgaagagct gctttgattt tttcagcata ttcttcaaag tgttcttctt tgttcatgta
    26941 tggtgtcatt acgctggcac gcaatacagt tacttttccg gcacggttcc attcttcatc
    27001 tgagaatcct agttggttca caaattgtaa tgggctgttg ccatagtctg ggatagcaaa
    27061 gtcagtatgt gaagttaaga actcattacc gtaaatgctt cctttcacgt atgaagaata
    27121 atcatatacg tcatggttca atttattcat tgcaactaag tcatcgttgc ctttttcttt
    27181 gaatacatag tctaccatat tgaaatctgg ataagttaat ggatgaactt cgatttcttt
    27241 gtctcctact ttaaatgaca agtcattcaa gaagttgtag aaacgatgtg caccttcgat
    27301 tgatgcaccc attaatttac catatcctgt gacattcaaa ggtaagacat ggtgtgctgc
    27361 ccaaacgctg gctgctgttg cccctgcttt tgaaccttcc aagatataag ccccaagtaa
    27421 tgctgggata tctgcgcctt tttcaaatac atatgtcgca aagtaagaga taacgtctct
    27481 catgcggata tctttgatga cgataccacc ggcagaatat ggaacatagc ccattttatg
    27541 tgggtcaatc gttacagatt ctgcttcttc gattgcttta tatgcactgt gtacttcttc
    27601 taagatataa tctttgtttt ctgtaaagac attgtatttg taatgaacat cttttaaatc
    27661 ttcaaatgga atgaagttgt tgtcttcgtc taagaaaatc gcacggccgt atccgccata
    27721 agcagcatct acgtgtaagt agaagtaaat accgtctttt tctaatacgc gacgcaatgc
    27781 aacgatttta tcgataccgt cgattgctcc ttcttctgtt gatcctacaa caccgactac
    27841 acctaggatt ggtgtttttt cagctgctag accacgaacg attttttcaa gttcgttgat
    27901 gtccatgcga tagttgtggt caactggcac tggaatcact tggtctaaac cgataccgat
    27961 gatgtcggca gcttttagcc atgaatagtg tttagtttgt ggaaccaacc attttcctaa
    28021 tttttctaaa tgttttccgc tacgtgctga gtgagctttg atttcgtcga ttttttctgg
    28081 gacgctgtct aacagattca tgatttcttc tgttgacaag ttcattaatt cccaatcgct
    28141 ttttccagca actagttcag gtgtcacttc tttcattgca agcggcaatg atttgatgtt
    28201 gcgggcatac caaagtcctt ctaagtttgc taaagaacca tcagcaacga tgtggcccca
    28261 gccatcttta tagctcatta attttgcaaa ttccattcct acttcttctt ccatttggct
    28321 tgttgcagga gaagattcat aggcaacgtt attaccattc catagcatag caaaattgta
    28381 cgctagtaaa gatggcatca atgtttcaga gttcatatga ccccagtaac gtccggcatt
    28441 atgccaagga acggaatggg tacgcatccg cgctgaaatt tctgatagaa catcctttgt
    28501 tttattcaca gtgtgttcaa aactagcact gcttttttct tctggcgtaa taataggcat
    28561 gtcttgaggc atgtagtttt gacgccagcc taaatgttca tccaccaatt cattcaacaa
    28621 agctttatag atttggccgt tctcagcttt gtccccaatg aatagggcat ttaggtttag
    28681 atctttcgac aatgattcac tcatagtgaa accctcctaa aaaaatattt aaattttgtc
    28741 gagccctaca ttacacctgt tcttctattc tgtaaactaa gttttggcct gatttttaat
    28801 catattttaa tatgtatcaa ttatccgaat tgttcacact tttgaaataa ttaaaaaaaa
    28861 cgagggaatt tacaaatgta aactccctcg caaagattgt attaacgaca accagcgttc
    28921 tattttagga tgggtttcaa aattggaatg ttgtaatagt atttcccttt ttcaaggtaa
    28981 aaacttacta aattctaatc aattactttg gccaaaaagt aatttttctt accttttcga
    29041 ataaccacaa acttaccatc gaattcatcg gatggattta tgacaaaatt taaatcggta
    29101 acgcggtcac cgttaatact tatagctccg tttgaaacat cttcacgtgc ttgacgttta
    29161 gatggttcaa tttttgtgga aacaagcagt tccacgatat tttcaggtgt gcttgaaatt
    29221 tcaacgttcg gcattttacc gaatccttgc gcgatttctt ccgcatttag ttctttaata
    29281 tttccagaga acaatgcttg cgttattttt tgcgcttctt tcaagtcttc ttcactatgc
    29341 acaaatcttg taacttcttc tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc
    29401 tctacttttt tcgctaagtc ttcgatttct tcttgagaca agaaagtaaa gaatttcaag
    29461 tatttcacta catcgcgatc atcttgattc aaccagaatt ggtagaactc aaatggtgaa
    29521 gttttcttcg gatcaagcca aacggcaccg cctgcagttt ttccgaattt cgttccgtca
    29581 gccttcagca tcaatgggat cgttaaaccg aacgcttctg cttctggtcc ttctaatttt
    29641 cggattaggt ctaatccagc tgtgatattt ccccattggt ctgctccacc gatttgcaat
    29701 tgcacatcgt atgttttatg cagatgcaaa aagtcgatag attgcaggat ttggtaagta
    29761 aactctgtaa atgagattcc tacttccaaa cggctggcaa cgatatcttt tgctaacatc
    29821 gtgttgatgt tgaagttttt accgtagtct ctcaaaaatt caagtaaaga gatttttgac
    29881 aaccagtcgt agttgttcac aaatgtgata tttgcatctt taccaaacaa ttttctcatt
    29941 tgattggaaa gtgcgtccac gttatgttgc acttgttcca tcgtttgcaa cacacgttct
    30001 gttttgcggc cgcttgggtc gccgatcgtt cctgttccgc cgccaatcag aatatatgga
    30061 tgatggcctg ccaattggaa tcttttcatc atcataaatg gaatcaaatg tccgatatgc
    30121 atcgaatcac cagttggatc aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc
    30181 tcgcgaagtc tttcttcgtt tgtttgctga ttaatggcgt cgcgccaagt taattcatca
    30241 ataatattca ttttactgac ctccgtgtag tttttttatg aaagcaggaa cctgctagca
    30301 tatgtaatta attatttctt caggatagga taggaaataa aaaaagtccc ttggcatata
    30361 agccaaggga cgaaatctcg cggtaccacc caatttgtat gtctagatat ccaacatacc
    30421 tctcgaacat ttaacgctgt cgcgtggcaa tcactgccaa agactcctga aatgtaattc
    30481 gctttaaagc atgtactggt ttgcagcgac caccagcttt ctgaaacatt ggctttaaca
    30541 actacttcgt ttcgatcttc gtcttgttat tattaatatg tgtctatcat agagcgcaac
    30601 tttttctttg tcaacagaaa aaggatattt tttttaaatt cctcattttt tcttttttga
    30661 atccgctcta attccgaaca ttttattttc aattattgtt aaaagaaata actttttctg
    30721 tattttttct gcaatttatc gagaagtcac tttactttgc ctcttattct ttcttcgtta
    30781 caataaggaa tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa
    30841 agcaaaaaat tattttagga gccacttctg ctttaactat cgttatttta attctaagtt
    30901 tgttttttgt catccgatca tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa
    30961 cagaaaaagc aaaagaaccg acagatgata agcaacaact agttactcaa gcaaaaaatc
    31021 tggctcaaag ctacgattat gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg
    31081 gatctactga aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg
    31141 actggtctga tcctactcag atctctcatg tctttttcca cagcttgatc gtggacccgc
    31201 aaaaagcctt ccactctcaa caggcacaag gatacaaaga ttatatggtg actgttgaag
    31261 aattcaaccg ttcgatcgaa caactttatc aaaatgattt tgttttagtt aatttgaatg
    31321 atttgatcca aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg ctacctgaag
    31381 gaaaaaaacc attggttctt tcacaagatg atgtcagcta ttatgaatat atggataatt
    31441 ccggttttcc tagccgattg gtcgtatcaa ataacaatga aatcaaaaat gtctataaag
    31501 agaaagataa agaagaaatc ggtgactttg acatggttcc tctgatcgat gcctttgtca
    31561 agaaacatcc tgatttttct tatcgaggcg caaaaggtac cttggccctg acaggttaca
    31621 acggagtttt aggttatcga acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa
    31681 aagagattaa agaagctaaa aaagtggcgg aacagttgaa gaaagacggc tggagctttg
    31741 cttcccatac atggggtcac ttgaatatga ctcaagcttc actagaagat gtaaaaaaag
    31801 acaatgaatt gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat attctgattt
    31861 atccatttgg tgcagacatc agcgactggc agccttattc agcagaaaat caaaaatttg
    31921 cttacttgaa gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct acaccagctt
    31981 ggggacaatt aggtacggat ttctatcgaa atgctcggat caatatcgat ggcattcgtt
    32041 ttcaatctga tttagacgga aaaaatccta ttcttgatcc attcatcaat gtccatgatg
    32101 tctatgacaa agaagcacgc gaatctgctt gattttacgt catttatgaa ccaaaaaagc
    32161 cgaactgaca ttttatcgtc agtttcggct tttttatgct ttatttcttc ttcggtgcga
    32221 ttcccttgat caggacttct gtccattctg ctgtatatgg aagcagtttt gcgtatatct
    32281 ccggcttaga aattccttct accattgctt gaaaatagag gatcaagaac tcatccgaat
    32341 atttcaaatc cacttttcct tctttccgcc ctcgatggaa aagatcaagc atgatactga
    32401 aactttcttg cgtatacgca tccatcgcgt cagaaaattc ttttttttct ttttttgtat
    32461 agtatgtcat caaatcgata taaaattgtt tgttcactcg attgagataa cggattttgt
    32521 tttcactcat ggcaatcagt gtttcttcaa atggaagatt ttccgcaatc aacgctttcg
    32581 cttcttgccc taaatcattc ataaatgcaa aaaatacttc ataaatcagc ccttccttgc
    32641 tgccaaaata tttaaaaatc gtcgctttac ttacctgagc gttctcagct accttatcca
    32701 ttgtcactcc agcggttcct atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct
    32761 ctttcttttt ctcttgtgct ctcttttcaa aaccgttcat ttgtagagcc tcccgtaatt
//
LOCUS       DACCXW010000016        32533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCXW010000016 DACCXW010000000
VERSION     DACCXW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.016"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            131..505
                     /locus_tag="IVY71_000614"
     CDS             131..505
                     /locus_tag="IVY71_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7984686.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVY71_000615"
     CDS             complement(619..1248)
                     /locus_tag="IVY71_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7984687.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVY71_000616"
     tRNA            complement(1382..1454)
                     /locus_tag="IVY71_000616"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IVY71_000617"
     tRNA            1668..1741
                     /locus_tag="IVY71_000617"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IVY71_000618"
     CDS             1696..1845
                     /locus_tag="IVY71_000618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984688.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IVY71_000619"
     CDS             2338..3666
                     /locus_tag="IVY71_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7984689.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IVY71_000620"
     CDS             3868..4701
                     /locus_tag="IVY71_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7984690.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IVY71_000621"
     CDS             4717..5454
                     /locus_tag="IVY71_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7984691.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IVY71_000622"
     CDS             complement(5609..6577)
                     /locus_tag="IVY71_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7984692.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IVY71_000623"
     CDS             6809..7642
                     /locus_tag="IVY71_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7984693.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IVY71_000624"
     CDS             7676..8515
                     /locus_tag="IVY71_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7984694.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IVY71_000625"
     CDS             8541..9101
                     /locus_tag="IVY71_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7984695.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IVY71_000626"
     CDS             9300..11021
                     /locus_tag="IVY71_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7984696.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IVY71_000627"
     CDS             11186..12328
                     /locus_tag="IVY71_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7984697.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IVY71_000628"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IVY71_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7984698.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="IVY71_000629"
     CDS             14128..14775
                     /locus_tag="IVY71_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7984699.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15168..17813
                     /locus_tag="IVY71_000630"
     CDS             15168..17813
                     /locus_tag="IVY71_000630"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7984700.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="IVY71_000631"
     CDS             18123..19436
                     /locus_tag="IVY71_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7984701.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="IVY71_000632"
     CDS             19426..20079
                     /locus_tag="IVY71_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7984702.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /locus_tag="IVY71_000633"
     CDS             20134..20826
                     /locus_tag="IVY71_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7984703.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="IVY71_000634"
     CDS             20976..21176
                     /locus_tag="IVY71_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7984704.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="IVY71_000635"
     CDS             complement(21430..23109)
                     /locus_tag="IVY71_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7984705.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="IVY71_000636"
     CDS             complement(23111..23323)
                     /locus_tag="IVY71_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7984706.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="IVY71_000637"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="IVY71_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7984707.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="IVY71_000638"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="IVY71_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7984708.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="IVY71_000639"
     CDS             27298..27495
                     /locus_tag="IVY71_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984709.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="IVY71_000640"
     CDS             27648..28133
                     /locus_tag="IVY71_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7984710.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IVY71_000641"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IVY71_000641"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7984711.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="IVY71_000642"
     CDS             29010..29537
                     /locus_tag="IVY71_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7984712.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="IVY71_000643"
     CDS             29628..30365
                     /locus_tag="IVY71_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7984713.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="IVY71_000644"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="IVY71_000644"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7984714.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="IVY71_000645"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="IVY71_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7984715.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10771 a         5522 c         6874 g         9366 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaagt tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DACCXW010000017        32367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCXW010000017 DACCXW010000000
VERSION     DACCXW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.017"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            6..2171
                     /gene="acm"
                     /locus_tag="IVY71_000646"
     CDS             6..2171
                     /gene="acm"
                     /locus_tag="IVY71_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP7984716.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2443..2928)
                     /locus_tag="IVY71_000647"
     CDS             complement(2443..2928)
                     /locus_tag="IVY71_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7984717.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3408..4049
                     /locus_tag="IVY71_000648"
     CDS             3408..4049
                     /locus_tag="IVY71_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7984718.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4068..4703
                     /locus_tag="IVY71_000649"
     CDS             4068..4703
                     /locus_tag="IVY71_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7984719.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4721..5536
                     /locus_tag="IVY71_000650"
     CDS             4721..5536
                     /locus_tag="IVY71_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7984720.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5855..7291
                     /locus_tag="IVY71_000651"
     CDS             5855..7291
                     /locus_tag="IVY71_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7984721.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7480..9912
                     /locus_tag="IVY71_000652"
     CDS             7480..9912
                     /locus_tag="IVY71_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7984722.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10142..11209
                     /gene="rlmN"
                     /locus_tag="IVY71_000653"
     CDS             10142..11209
                     /gene="rlmN"
                     /locus_tag="IVY71_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7984723.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11402..12505)
                     /locus_tag="IVY71_000654"
     CDS             complement(11402..12505)
                     /locus_tag="IVY71_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7984724.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13057..13605
                     /gene="aac(6')"
                     /locus_tag="IVY71_000655"
     CDS             13057..13605
                     /gene="aac(6')"
                     /locus_tag="IVY71_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7984725.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14151..15812
                     /locus_tag="IVY71_000656"
     CDS             14151..15812
                     /locus_tag="IVY71_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7984726.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15882..16244)
                     /locus_tag="IVY71_000657"
     CDS             complement(15882..16244)
                     /locus_tag="IVY71_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7984727.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            16312..17253
                     /locus_tag="IVY71_000658"
     CDS             16312..17253
                     /locus_tag="IVY71_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984728.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17254..18297
                     /locus_tag="IVY71_000659"
     CDS             17254..18297
                     /locus_tag="IVY71_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984729.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18303..19373
                     /locus_tag="IVY71_000660"
     CDS             18303..19373
                     /locus_tag="IVY71_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984730.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19363..20316
                     /locus_tag="IVY71_000661"
     CDS             19363..20316
                     /locus_tag="IVY71_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7984731.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20328..21338
                     /locus_tag="IVY71_000662"
     CDS             20328..21338
                     /locus_tag="IVY71_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7984732.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21623..22444)
                     /locus_tag="IVY71_000663"
     CDS             complement(21623..22444)
                     /locus_tag="IVY71_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7984733.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22541..22819)
                     /locus_tag="IVY71_000664"
     CDS             complement(22541..22819)
                     /locus_tag="IVY71_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984734.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23087..25096
                     /gene="metG"
                     /locus_tag="IVY71_000665"
     CDS             23087..25096
                     /gene="metG"
                     /locus_tag="IVY71_000665"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7984735.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25575..26162)
                     /locus_tag="IVY71_000666"
     CDS             complement(25575..26162)
                     /locus_tag="IVY71_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984736.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26337..27659
                     /locus_tag="IVY71_000667"
     CDS             26337..27659
                     /locus_tag="IVY71_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7984737.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27814..28590
                     /locus_tag="IVY71_000668"
     CDS             27814..28590
                     /locus_tag="IVY71_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7984738.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28583..29152
                     /gene="rnmV"
                     /locus_tag="IVY71_000669"
     CDS             28583..29152
                     /gene="rnmV"
                     /locus_tag="IVY71_000669"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7984739.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29145..30029
                     /gene="rsmA"
                     /locus_tag="IVY71_000670"
     CDS             29145..30029
                     /gene="rsmA"
                     /locus_tag="IVY71_000670"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7984740.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30480..31109
                     /locus_tag="IVY71_000671"
     CDS             30480..31109
                     /locus_tag="IVY71_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984741.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31233..32342)
                     /gene="ugpC"
                     /locus_tag="IVY71_000672"
     CDS             complement(31233..32342)
                     /gene="ugpC"
                     /locus_tag="IVY71_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7984742.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT        10786 a         5648 c         6700 g         9233 t
ORIGIN      
        1 aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa
       61 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
      121 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
      181 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
      241 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
      301 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
      361 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
      421 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
      481 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
      541 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
      601 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
      661 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
      721 aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa
      781 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
      841 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
      901 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
      961 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
     1021 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
     1081 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
     1141 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
     1201 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
     1261 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
     1321 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
     1381 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
     1441 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
     1501 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
     1561 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
     1621 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
     1681 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
     1741 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
     1801 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
     1861 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
     1921 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
     1981 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
     2041 agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg
     2101 ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata
     2161 aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa
     2221 attccgattt ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc
     2281 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
     2341 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
     2401 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
     2461 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
     2521 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
     2581 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
     2641 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
     2701 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
     2761 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
     2821 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
     2881 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
     2941 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
     3001 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
     3061 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
     3121 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
     3181 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
     3241 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
     3301 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
     3361 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
     3421 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
     3481 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
     3541 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
     3601 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
     3661 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
     3721 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
     3781 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
     3841 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
     3901 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
     3961 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcgta atgaaacaag
     4021 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
     4081 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
     4141 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
     4201 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
     4261 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
     4321 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
     4381 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
     4441 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga
     4501 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
     4561 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
     4621 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
     4681 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
     4741 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
     4801 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
     4861 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
     4921 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
     4981 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
     5041 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
     5101 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
     5161 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
     5221 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
     5281 gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
     5341 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
     5401 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
     5461 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
     5521 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
     5581 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
     5641 acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa
     5701 aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc gactttttca
     5761 caacctcctt tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag
     5821 gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt
     5881 tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca
     5941 agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt
     6001 taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc
     6061 tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt
     6121 tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga
     6181 cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca
     6241 agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag atttcactca
     6301 ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa
     6361 tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac
     6421 agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat
     6481 caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc
     6541 aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct
     6601 gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag
     6661 gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga
     6721 atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa
     6781 tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaaa gttcatcaga
     6841 agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa
     6901 ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg
     6961 ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga
     7021 agataaacaa atccatgacg actatcgaat caattactta caaagccacg tcaaagccat
     7081 caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga
     7141 tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga
     7201 cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa
     7261 actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc
     7321 tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat
     7381 aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt
     7441 tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct
     7501 actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg
     7561 ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta
     7621 ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa
     7681 atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt
     7741 tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta
     7801 ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg
     7861 acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt
     7921 gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt
     7981 tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc
     8041 tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt
     8101 gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca
     8161 gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaatatga gaagaaaaaa
     8221 gcggtgagcc agcaaaggga aaatactgct gatgaatggc aggaaccaga aaatacagag
     8281 ccggagcagc taagttttgt tccaatcgac agttttcaag aaaatatcca tcctgcgaat
     8341 ctagagaaac cagtaccaga tacaccaaaa cagaccaata cagcagaagg tttttcagat
     8401 gagttgccag aggatgacgg tacctcatta gaatttgaga ttgaagcaga acaggaaaat
     8461 caagactatg aattaccgac agttgatttg cttgattcta taccaactgt ggatcaaagt
     8521 gatgagtata aaaaaattga aaaaaatatc ggcgtattag aacaaacttt ccaaagtttc
     8581 ggtgtcgatg ccaaggtcgt caaagccagc ttaggaccat ccgtcacaaa attcgaagtc
     8641 cagccggctg taggggtaaa agtaagtaaa attgtgaact taacagatga tatcgcgctt
     8701 gctttagcgg cgaaagatgt ccggatggaa gcaccgattc caggtaaatc actgattggt
     8761 attgaagtac caaatggaaa gatcagtatg gtttccttta gagaaatcat cgaagcacaa
     8821 ccaaaccacc cagataaatt actagaagta cctttaggac gtgatgtttc tggacgggta
     8881 caaacggctg atctttcgaa gatgcctcac ttactcgtgg ctggttcgac tggtagtggg
     8941 aagtcagtag caattaatgg gattatcaca agtatattaa tgcgtgctaa gccccatgaa
     9001 gtaaaactga tgatgatcga tccgaaaatg gttgaattga atatgtacaa cggcatacct
     9061 catctgttga cgccggtcgt gacgaaccct agaaaagcag cgcaagcctt acaaaaagta
     9121 gtacaagaaa tggaagaacg atatgaaaaa ttcgcggcaa ccggagtaag aaatatttct
     9181 ggttacaatg aatttgttca acagaaaaac ttggaaaacg gaacgaaaca tccaacctta
     9241 ccattcatcg tagtgatcgt agatgaatta gctgatttga tgatggtagc cagcaatgaa
     9301 gttgaagatg cgattattcg tttagcgcaa atggctcggg cagcggggat tcatatgatc
     9361 ctagctactc aacgtccaag tgtggatgtt atcacaggaa tcatcaaagc aaatgtacct
     9421 tccagaatgg catttgcggt atcaagcggt acagattctc ggacgatcat tgattctaat
     9481 ggggcagaaa agctcttagg gcgaggagat atgctattct tgccaatggg tgagaataaa
     9541 ccgatccgtg tgcaaggagc attcatttct gaccacgaag tagaacgggt cgttcaattt
     9601 gttacagatc agcaagaagc tcattatgaa gagaaaatga tgccgacaga tgaagttgaa
     9661 acgacaggtg ctccagaaca gccacaagat gagctgtttg aagaagcaaa agcacttgtc
     9721 gttgaaatgc aaacggcaag tatttccctt ctccaaagaa gatttcggat cggttacaac
     9781 cgagcagcac gtttagtaga tgaactggaa gctcatggag ttgttggccc gtcagaagga
     9841 agcaagcctc ggaaagtatt cattgagcag gaagaaacat cagaaacgtt gccggacgat
     9901 aatgacatgt aaaacttgtg tgatgaaggg ttgaaaaacg aataaccaat aaaaagacaa
     9961 ggcatgatga taccaaaaga aaaaatggtt tttttccatc ttttctaata cggtatgatg
    10021 tcttgtcttt ttagtttttg tgagattaaa gttctattcg atttatttgt gaagtattgc
    10081 attaaggaaa tgaatcatgt atagtatgta gcgaaacttt gagaaaaaga gggaaaatac
    10141 tttggaaaaa cagtccattt atggcttgac gaatgaagaa ttaatcaatt ggtttataga
    10201 aaacggcgag aaaaaattcc gagctgccca agtttgggaa tggttgtatc aaaaacgtgt
    10261 aagtaatttc acagaaatga cgaatctatc gaaacaattg atcgaaaaat tatcggagca
    10321 ttttatcatc aatcctctaa aacagatggt cgtccaagaa gcttcggatg gtacagtaaa
    10381 atatttgttt gaactccctg ataaaaacat gattgagact gtactgatga gacaagaata
    10441 tggcttgtca gtttgtgtga cgacacaagt aggatgtaat atcggctgta cgttttgtgc
    10501 aagcgggctg ctcaaaaaga accgtgatct gactgctgga gaaattgttg cacagatcat
    10561 gatggtccaa cattattttg atgagcgcaa tcttggcgaa cgcgtttcac atgtcgttgt
    10621 aatggggatc ggtgagccat ttgataatta cgacaatgta atggactttt tgcatatcat
    10681 caatgatgcc aaaggattgg caattggtgc tcggcacatt acagtttcca ctagtggatt
    10741 agcacacaaa atcaaggaat tcgctaataa cggcttgcaa gtcaatctgg ctatttcctt
    10801 gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg atcaaccgaa gtttcccaat
    10861 tgagaaattg atggaagctg tggatgagta tttagagaaa acaaaccgtc gaatcacgtt
    10921 tgaatatatc atgttgaatc aagtaaatga tagaccagag catgcgcaac aattagcaga
    10981 tctattaaaa gataagaaaa aattagcata tgtcaacttg attccatata atccagtcag
    11041 tgaacatgat caatattctc gcagtccaaa agctgatgta ttgagatttt atgatgtatt
    11101 gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat ggtacagata ttgatgcagc
    11161 ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt gccaaataat cgtatcctac
    11221 tcgttttttc aattaccata aaaggagcag ttttcttaat taaggaactg ttcaacttaa
    11281 ataagagaaa agattctata gcgaaagcta taaagctttt tttgacaaga aaaaccctca
    11341 tcaagcaaag aaatgtcctc aatttgatga cagcctgctt gatgggggtt ttagcaatta
    11401 tttaactgat tttcttatat ccttgctggc tttcttgtgt agcatgataa gtttctacgt
    11461 caagaatggc ctgtttaaat ttttctcggg tgatttcata aggcacatgt ttaatatcat
    11521 ttgttgccat ggctttatct aagattgctt ctaaatcggt cgtgacattg agattcatgt
    11581 cggttaacga tactggaagt tggttctttt tcataaacac atagatttta tcaaacaaat
    11641 cgaactgctg atccatcatc aataaggtca gtacaccata actgacagat gctccgtgaa
    11701 ggtatccctc cattttttct agaacagtcg taccgtaata gaatgagtgt gccatagcag
    11761 aattatagtt attgttaact aaaacagaaa ccaatccggt agtaacaatg atatccgaaa
    11821 cgacttgttc aattgcatat gagggacgat cgtcggcact atctttcaat gcttgcgtac
    11881 cataagtcaa gagacgatca atgcacattt ttccgatttg gattcctaat ctatccgtgt
    11941 agcctagatc atcgccacga gcagaaaaag tagattcgac ttctttggat aaagcatcgc
    12001 caatacctgc ccataagtat ttatcaggtg cttcggcaat aattgctgta tcaatgaagg
    12061 tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat tacgccttct tcgttataga
    12121 tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat agtgggaaag gtgaagactg
    12181 gtttagctag tttttcacca atgactttta cagtatcgca agcacggcca ccaccgactg
    12241 caaacaggac gtctgctttt tggacacttt catgctggat caactgttca atatttccat
    12301 aagttgaatt acccccgtac cagagactgg cgacaacctc tatttcctgt gtcaaagcta
    12361 cttttatttt gttttctgcc tttgaaagtg cagtcttccc tccaatcagc actacttttg
    12421 tcccatattc tttgacgatt ccatttgctt tttcatacgc atctgttcca atggtataat
    12481 ttggtaagaa aacactttga ctcatattca tttccttctt tcttcgttga aacgggttaa
    12541 aattttaaag agtgatcgat ctgactgcgg ctgtagatgg agtagaggtg aacctaagac
    12601 aggataagtg atgaataagg tggcatgaac accttgaaat catcaataag tatcctaaaa
    12661 acgaacaagt acaaaaacat tgaaaacgac aataatgaat ttttgttcct cttagtattt
    12721 ctaccgattc cctttgcttt tttgtaatta tctaaaaatt ctaaaaatta agattattat
    12781 atgaaaacag gttttatttg tcaattcata attggttttt tttaaagcaa aagataatct
    12841 atatcatgat gaacaaacag agttttttcc tccaattgat ggctttcctg atctttccta
    12901 ttttgaataa agtgtttctg tataattttt tattttataa gtaagataaa taatgaaaga
    12961 atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag
    13021 gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt
    13081 aataatccag tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa
    13141 tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta
    13201 gcagcggtag accaagatga gttagtagga tttattggtg caatccctca atacggtatc
    13261 acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt
    13321 actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat
    13381 ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat
    13441 acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa
    13501 aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat
    13561 atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg
    13621 acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt cttgaatttt
    13681 tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta ccagaggttg
    13741 tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttctacatt
    13801 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt attcagataa
    13861 gggactgtga caagactttt gtcacagttt ctttttgttt attctttttt atctttgtat
    13921 ggcaataata gacaatataa cgaaaattat ttaatggctt ttggaagacc atggaaaacc
    13981 ttgtacaaag ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa tcattatttt
    14041 atgaaaaaga caaaaaaaag tgttgatttt ttaataatta ttcattaaac tcttaaacaa
    14101 cttcagaaaa tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga
    14161 aatttacatt tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca
    14221 gttcagccac aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta
    14281 acttagtcgt gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca
    14341 gttttacagc attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac
    14401 cacagccggc aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa
    14461 tcaaattacg agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg
    14521 tttatggctg gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg
    14581 cgccagttga aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga
    14641 tcaaagcagt tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc
    14701 aatatctgct tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg
    14761 gggatgccta tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag
    14821 attttgatgg accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg
    14881 acaaagatac agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg
    14941 cattgaactt attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac
    15001 agcaaaacgc aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg
    15061 aattcaatca acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt
    15121 cttattctat cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct
    15181 ctacaggatt gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa
    15241 aagaagctgg aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag
    15301 cgaaaaaaga attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt
    15361 catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa
    15421 cagtcaaacc aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt
    15481 ttgacgcagt actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt
    15541 tgtttgtcac aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag
    15601 cagttaaagc agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc
    15661 aaaaagcaat caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag
    15721 aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat
    15781 gggattacaa atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa
    15841 cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa
    15901 agcatgaaaa tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac
    15961 ataccgtagc tggcagacga gagcgtggaa aattctggtt ttttctttcg gttccttgtt
    16021 cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa
    16081 aaaccgccga taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg
    16141 agtgtaaagg aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct
    16201 actgcgtagg ggatagattt tttttcattt gattgctcct ttatttttgg ttcattatct
    16261 aataatgtag cgaaacatct gtcagaagtc aaaagctagg gggaaacatt agtgaatagt
    16321 tatatcaaat atgtattaaa acgtgtcttt ttcatgatca tcacgctttg gttaattgca
    16381 acaatcacct ttttcttgat gcaattgctg ccgggcacgc catatacgaa tcaggaacgt
    16441 ttgagtcctg aaacaatcga gatgctaaat aaacaagtcg gtttggataa accagttatc
    16501 gtacaatatg ggatttatct ttctaatttg cttcaaggag attttggaat ttccttccaa
    16561 tttaaaaacc agccagtcgc acatttgtta gctggaagaa tcggaccatc cttgcagtta
    16621 gggttacagg caattatctt tggaacagtc ctagggacga ttttaggaac gatctctgcc
    16681 atgaagcaaa atacatgggc agatacatct tctacactcg tagcgatatt aggtcgttcc
    16741 attccaaact ttgtatttgc tgttttatta caatatatct ttgcaatcaa attacaagtt
    16801 ttgccaatcg caaaatggga tggctttgtg tacacaatct taccaacaat tgcgttggcc
    16861 atgtcgccgc ttgctgactc tgctcggttt atccgtacgg aaatggtgga agtactgcat
    16921 agtgattatg tggaattagc aaaagcaaaa ggattgagcc gttgggaaat cgcctttaaa
    16981 catggtcttc gaaatagttt gattccattg atgactttat taggaccgtt agctgtagca
    17041 ttgatgaccg gatcactggt ggtagaaaat atctttgcta ttcctggtat cggggaacaa
    17101 ttcgttaagt caatcacaac aaatgattat cctacgatca tggctgtaac gatcctttac
    17161 tcatttatgc tgatttttgt tattttgatc gtggatctat tgtacggtct agttgatcca
    17221 agaattcgtg tttcagaagg gagccgagga taaatggaaa tgataccaaa gcgttatgaa
    17281 acagttgcag atatccctgc ggatgaattt cttccattac aaaaaaatac cgaagaagaa
    17341 cgtgaacaga ttactgcacc ttctttgaat tttatccaag attcatggcg ccgtttaaag
    17401 aaaaacaaag ctgccgttat ttcaatggtt ttactattgg tcatcatttt gatctctgtg
    17461 atcacaatct ttgtttctcc tcatgatcca actgcccaaa acgtggctta tatcaatttg
    17521 cctcctcgta ttccgggaat caatatcgat ggattgaatg gaaaagcaat ggttggtggt
    17581 gaacttgttg ataaatatgc tcaagcaaac gtcccttcaa acgtaaattt ctttttagga
    17641 acagacggtt tgggacgtga tgtattaagc cgcttattta tgggaatgag aatctcgttg
    17701 ctgatcgcat ttattgctgc attactagat gtaacgattg gtgtagctta cgggttggtt
    17761 tctggtttgt taggcggacg tgtcgataac gtgatgcaac gttttttgga aatcttatca
    17821 ggaattccaa acctcgttgt catgattttg atgttggtcg tttttgaacc aggaattttt
    17881 tcaatcgtgg ctgctatggc catcacaaac tggataccaa tggcacgtat cgttcgtgca
    17941 caaacaatga aattaaaaga tcaagaatat gttttagctg gattgacatt aggtgaatca
    18001 agaatgaaaa ttgcattcaa acatattttg ccgaatatct ctagtgtcat tattattcaa
    18061 atgatgttta gtattccatc tgctattttc ttcgaagcct ttttaagctt tatcggttta
    18121 ggtcttacgc ctccgtcagc ttcattagga acgatgttga gcgaagggta caagacattc
    18181 ctgtatctgc cttatctatt atggattccg gcagtaactt tatcggttat catgattggc
    18241 tttaacttac ttgctgatgg attgcgtgat gcctttgatc ctaaaatgaa agagtgaaga
    18301 cgatgacgaa aattttagaa gtgaaagact tagaaatctc ttttgatacg tatgctggaa
    18361 aggtacgagc gattcgcggt gtcaattttc atttgaataa aggagaaaca ttggcgatcg
    18421 ttggggaatc tggaagtgga aaatcagtga cgaccagaag catcatgcgc ttgctgtcaa
    18481 gcaacgccaa tatagatgct ggtcaaatcc tctttaaagg taacgatatc gttggaaaat
    18541 cagaaagaga gatgcaaaaa atccgtggta aagaaatagc gatgattttc caagatccaa
    18601 tgacctcttt agatccgacg atgcccattg ggaagcaagt cgctgagtcc cttagaaagc
    18661 ataataaagt ttcaaaaaaa gagggacaaa aagctgcatt agaattgctt aaattagttg
    18721 gaatacccga agctgagaaa cgtatcaaca gttatccgca tcagttctca ggaggtcagc
    18781 gtcaacggat cgtgatcgcg attgcactgg tatgttatcc ggaaatcctg attgctgatg
    18841 aacctacaac agcgcttgat gtgactattc aagcacagat tttggagctg ctgaaagaaa
    18901 ttcagacaaa aatcgataca tcaattatct ttatcactca tgaccttggt gtagtagcga
    18961 atgtcgctga tcgtgtcgct gtcatgtatg gaggaagaat cgttgaagtt ggaacatcgg
    19021 aagaaatttt ctacaacccg caacatccct atacttgggg actgcttggt tcaatgccga
    19081 cattagatag tgcgaacgat cgattgtatg ctattcctgg ttctccaccg gatttattga
    19141 acccgccaaa aggagacgca ttctatccaa gaaatgaatt cgcgatgaaa atcgatgcag
    19201 aacaagaacc accatttttt gaactatcaa aaacacacaa ggcagcaact tggctacttg
    19261 ctccgcaggc acccaaagtg acaccgccag aagaaatcca acgtcgctgg gctatcttta
    19321 aagagaaaca aaaacatact tacggtcaag gaggaattgc tgatgccaaa taaactactt
    19381 gaagttaaag gattgaaaca gtatttcaat gtaggaaaaa agaatgaagt acatgctgta
    19441 aatgacatca gctttcacat ttatgaagga gaaacattcg gtttagttgg agaatctgga
    19501 agcggaaaat ccactactgg ccgtacgatc attcgcttga atgaacctac tgatggtgag
    19561 atattattcg acggtcaaga tataacgaag atcaaagata aaaaaggttt gacgaaattc
    19621 cggcatgatg ttcagatgat ctttcaagat ccatacgctt cgttgaatcc tcgtatgaaa
    19681 gtcagagata tcattgccga aggaattgat gttaatggac tagctaaatc atctgaagag
    19741 cgtgcaaaaa aagtcgatga tctattgaga actgttggat tgaatccaag ccacggtact
    19801 cgttatcctc atgaattttc aggtgggcaa cgccagcgta ttggtattgc acgagcattg
    19861 gcagtaaaac cgcgttttat catttgtgat gaaccaattt ctgcactaga tgtgtcgatc
    19921 caagcccaag tggtcaattt gttgcaagat ctccaaaaag aacatcaatt gacctatcta
    19981 tttattgcac atgatttgtc gatggttaaa catattagtg accgaatcgg tgtaatgcat
    20041 aacggcttgt tattagagat gggaacaagt gatgaaatct ataatcacgg tgttcatcct
    20101 tatacagaaa gtttgttatc tgctattcct ctaccagatc cagaccatga gagaaaacgc
    20161 agaagaatca aatatcaacc tgaaccagat gacggacaag tacgtcaact tcgtgaaatc
    20221 gcaccagaac attttgtcta tgcaactgag caggaagtcc catattacgc aaaaaaacta
    20281 aaacgacaaa aggaatctct tctggttgct aactaggagg gacatagatg ctaaaagctt
    20341 ttaaattccg tatgtatccg acagaagaac aaaaacagca attgattcgt acttttggat
    20401 gtgctcgatt tacctataac catttgctga aaaagcggca gaaaagttgg caacaaacag
    20461 gagtagccaa tttttcgctc acacccgcta cattaaaaaa agaataccca ttcttaaaag
    20521 aagtagacag tcttgcttta gcaaatgcac agcttaattt ggatcgggct tttcgcaatt
    20581 atttcaaggg acgtgccagc ttcccaaagc ttaaaacgaa aaaaagtatg tggcaatctt
    20641 atacgaccaa caaccaaacc cgtacgatct atctggaaaa tggctactta aagttaccaa
    20701 aacaaaaaga actaatcaag ataaatcagc atcggccagt tgaaggatcg attcgctccg
    20761 ctacgatttc tgctcgatat aatgaagaat tttatgtcgc actactttgt gatgtttcgc
    20821 cagtaaaaaa agaatcttta gctaaatgga tcgggatcgc ctatcatccg aagacattga
    20881 tccaaacatc tcgacctcta gaagtaactt taccaaaatt tcaccagaca gaagaaaagc
    20941 tgcaacatgc acagcggaaa ttgaatgtga aagtccgctc cgctcaccat agaaagatca
    21001 ggttagatca ggcaagcaat tatcagaaac aaaagagaaa agtaatggat ctttatctga
    21061 aacaaaagaa ccagcgagaa gattatcttg aacaattatc tgggaaattg gtcaaacaat
    21121 atgactactt atttgtagaa tcttttccaa aagaagaggc acatgctgat ttttctattc
    21181 acgactggca taaattaata acaaaattgc gttataagtc ccaatggtat aataaaaaat
    21241 ttcttttgat caataccgac ggagcagaag aaagcaattc cgtaagaaag agccaagtag
    21301 tagaaaaaat gggcagacat tcggttatca agggataaaa aatgaatgaa actgatagga
    21361 gcgttagggt tagccttgtt aataaaaagt cttaatgact tttgtttcta ggaatttttc
    21421 ttttgaagaa aagctcacta attatgacaa accaataaaa caatagcaga aaaagttcgt
    21481 acgagctttt tctgctattg ttaatttttt attcagaaag tttacgaata catcaaaatt
    21541 ctgaaacaaa aaacgtaact cacaaagagt tgtttttgcg ttttttcttg aatgattagg
    21601 tctgaatctt ttgctcacaa ccttattcat ggtgccccgg cttgagctga tagatttttg
    21661 aaggacggcc gattcctaca ggacgttcac cgatctcatc gaaatattgg agcatagctt
    21721 tcttaaaatt ggaatgatcg atcgttttat aatcgatccc taagaattta gcaaatactt
    21781 ttcgagcttc cgtaatcgtg aaatctttac ctaaaacttg tagaacttgc ggctcgtgtt
    21841 ccattttatt gactacacgg ttgaatgctt tcaagatgat ttgactatgg tcaaaagcca
    21901 aagtatcttt gccagaagac tctccagtga ctaaatcaag cgagatagcc acttctccgc
    21961 tagataaatg gatcttgtta ctatgacgtt ctagtgtgaa ccagcgtact tccttggcat
    22021 catctccggc gattagcggt tcttctccaa taaaggctaa gtagctgact gtgacgaccc
    22081 agcctcgcgg atctcgatta ggcgtactga atgtatgcag ttgctcaata ttttcttttg
    22141 aaataaccac accagtttct tcctttgttt ctcttaagac gctttctcca gttgactcat
    22201 ccttttggac aaagccacca ggcaatgccc atgaatttcg ataaggatgc ccttttcttt
    22261 ggatcaacaa aactttcagc tgatcttctt ctttgttata gcacatcagt acgatatcga
    22321 cagtcaaaga gggcttgtca tattctggac gttcctgttt ttgataccag ttaagaaatt
    22381 cttcttcact tgctttatgt tcataatata atttttcttc ttctttagaa gcaaaacggt
    22441 tcatctgacc acctccattg acagtaaaag tactttttac cttattgtag cagactctga
    22501 aaaaaaacca aggatttact tgaaaaatct aggttatcat ttaaatatga aaaataaatg
    22561 gaagtatgag attgaataaa aagaaaagca gataaaacgg caatacttta taccgctctt
    22621 cacgaatggc aaagaagcca caaataacga tatatacgtc aatgataaaa cgcaaaatcc
    22681 aagctgctgt cataaaactg ttcaaatgcc catggaggtt aaaataacca tacatacgaa
    22741 tataaaaata gctgaatatt aaatgtgcta atgaaagaaa agcaatacta agtgtaaaaa
    22801 aaagatgttt cttctgcatg attgttccag tcccttacta agatagatag tctttattat
    22861 accacgtgtg aggggagaaa taaaaagccg ttttttagta gatatgaaac aaaacaaaaa
    22921 cccgcattta ttcaacctat tcatcctttt aaaggataga ttgaacgtag atagaagtcg
    22981 tttatccagt ctttttcatt ttgaaaaaaa gctaacagca tgcttttttt atggtatact
    23041 agcaaggaaa cgaattcgca tcatgcgata aaggaggaag agccccgtgg cagaaaagga
    23101 aactttttat atcactacgc caatctatta tccaagcggc aaacttcata tcggcaactc
    23161 gtatactacg attgcatgtg acgcaattgc tcgttataaa cggttgatgg gctttgatgt
    23221 gttttatctc actggagtgg atgaacatgg tcaaaaaatc gaaacaaaag cagaagaact
    23281 aggtgtaaaa ccgcaagaat atgtagataa aatggcggct gatgtcaaga aattatggaa
    23341 aacgcttgat atcagttatg ataaattcat tcgcacgaca gatgattacc ataaagcagc
    23401 tgttcaaaaa atatttgatc gtttattaga acagggagat atttacttag gtgaatatga
    23461 aggctggtac tctgtttctg acgaagaatt ctttacagaa acacaacttg cagaagtgta
    23521 caaagatgac gaaggtaacg tgatcggtgg taaagcgcca agtggacacg aagtagaatt
    23581 ggtaaaagaa gaatcatact tcttccgcat gagcaaatac gcagaccgtc tattggaata
    23641 ctatgaagaa catcccgaat tcatccagcc agaatctcgt aaaaacgaaa tgatcaacaa
    23701 ctttatcaaa ccaggattag aggatcttgc agtttcacga acaactttta cttggggagt
    23761 accagtcaag agtgatccga aacatgtggt gtatgtatgg atcgatgcat tatccaatta
    23821 tattactgca ctaggatatg gctccgaaga tgaaagtcta ttcgaaaaat actggcctgc
    23881 agatgttcag atggtcggta aagaaatcgt tcgctttcat acgatctatt ggccaatcat
    23941 gctgatggcc cttgatttgc ctctacctaa aaaagtattt gggcatggct ggctattgat
    24001 gaaagacgga aaaatgtcta aatcaaaagg aaatgtcgtt tatcctgaaa tgcttgtgga
    24061 acgttatggt ttagatgcac tccgttatta tttgcttcgt gctgttccgt ttgggtcaga
    24121 tggtgtcttt acgccagaag attttgtttc ccgtgtcaat tatgatctgg caaatgatct
    24181 agggaactta ttgaatcgaa cgattgctat gatcaacaaa tattgtgatg gactcgttcc
    24241 tgactatgcc tcacttgtca ctccatttga tagtgaactg tctacgacgg ctgctaacgt
    24301 tgtcggacgt taccatgatg caatggaaaa aatggaattc aatacagcat tggctgaaat
    24361 ctgggtgctg atttctcgtg caaacaaata cattgatgaa acagaaccat gggtattagc
    24421 aaaagacgaa gaaaagaaag ctgaattgaa tagtgtcatg atccatttag ctgaatcttt
    24481 gcgtatcgca gcgatattac ttcagccaat catgacagaa acgccagaga agatcttcaa
    24541 tcaactagga ttggactctg aaacaatgaa tcttgaagga ttacactttg gcgaatttcc
    24601 ttcaggaaca aaagtagttg caaaaggtac accaatcttc ccacgtttag atatggaaga
    24661 agaagttgct tttattcaag aaaaaatgtc tgaaggtact caaacaaacg aagatacagt
    24721 caaatgggat ccagaagaaa cagaacttgt ttcaacgaaa gaaaaacaaa tcaaatttga
    24781 tgtatttgaa aaagtagaac taaaagtagc agaagtcatc aactgtcaaa aagtagaagg
    24841 ggcagataag ttgctgcaat tccgactaga tgctggagac agccaagacc gtcaaatctt
    24901 gtcaggaatc gctgaatttt atcctgatcc aagcgaattg atcgggaaaa aagtcgtcat
    24961 tgtagcaaat ctgaaaccac gaaagatgcg tggacaaatc agtcaaggaa tgatcctttc
    25021 tgcagaagca ccagatggct ctttacaagt aatcgaagca ccaaaatcca tgccaaatgg
    25081 ctcagaaatc gcatagaaat tcaaaggggg tatgacacta gttgtgtcat accccctttt
    25141 tccgaaattt gagaagcaat tttcgtaaat tccttcttat tacttgaagc caatcatata
    25201 atacagaaac agagaactat ccaaaataaa tcctagaaaa agaagtacga gcttcgctga
    25261 tttttgttaa tttagaataa tactaatagg aatcgtaaaa acagaaaaaa agcataatga
    25321 taaagggtcg ggcagatttt aattgattca tctggtactc ttttttattg tccttacatt
    25381 cataaagttt gaatgcaatg actttattac atctatattt atatctaaca gtagcgttga
    25441 tgtgttaatt ataagttcta tcactttatg ttcaattagt ttgtgctctt atatgtgtga
    25501 ttgaaaatat agataaaaaa acaatcatcg gctttgactt cgatgattgt tttctttatt
    25561 ttgtaaaaat cctatcactt gattttatgg taacctttta tttttgtctt caaacggctt
    25621 ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga cataccagaa aatcgtacgt
    25681 ccgataccat aatgttgata gagctttgtc aacatcacgt gtaacaagat caaccctaaa
    25741 agcaaggagc cagctataaa ccaatgatta tcccaatgca tgacttcact aagtgccatc
    25801 actccaaaag gatagaaata aaaatagatc ccgactaagg tcatccgcca gttctttgta
    25861 aatgttccga tattcaataa aaagaaaatc gctgaaagca aaataccgat aggcggaaaa
    25921 agcccaacca aaaaggaata caacatcccc cacaagatca tcacaggacc tttgatcaat
    25981 gcaacgattg cgatgaacaa agtcagcaat attagttttt gtgataacgt tgcaacagtc
    26041 attaatccaa ataaaattaa tgtgaaccaa tgccaatggg actgctgttg ttctttacgg
    26101 gaaaaatcga cccggttgga taaataatct gctaatttcg gataagaacg cttaaaatcc
    26161 ataaactgtt cctccttcgt ggaaaaggta ggtttagtat acgaaaaata aaaaagaaat
    26221 acatccgtct taagagggag tttttcactg aaagtggaga aaaaagaatg gtaaattaaa
    26281 atattctcag ttatactgaa aattatacca aatttcaaag aagaaagagg atgggaatga
    26341 aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt agctaccgtt atgggaaccg
    26401 tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac tgctagtact cagtctacaa
    26461 gtttgacatt gctagcttgg cttttgggtg gtttcttaac gatctgtgct ggattgacag
    26521 tggctgaatt agctacagca atcccggaaa ctggtggagc tgtaaaatat attgaagctg
    26581 cgtatggaaa attgcccagc tttttattag gatgggcgca aagcttgatt tactttcctg
    26641 ctaatattgc tgcattatcg attatctttg cgacacaatt gacgaactta ttacaattat
    26701 ctaccgatta tcttttgttg attgcagtga tcaccgctgt ttctgtgaca gggttgaatt
    26761 tgttagggac aaaagtcggt gcttctgtac aatcagtgac gttgattgtt aaattaatac
    26821 caattgctgt gattgtcata tggggattat tgaccccagg acaaggaact gtccagttat
    26881 ttccaatcga agctggaaaa gatgttactt ttgtcgaagg attgagcagt gcgttgttag
    26941 caacgttgtt tgcttatgac ggctggttag gtgtcggtgc aatggcaggg gaaatgaaac
    27001 ggccggaaaa agatttgcct aaagcaatta ttcttggttt gagttttgta acagtcgttt
    27061 atctattgat caattttgtt tttttgaaaa ctttgccgat tgatcatctt gccggtaatt
    27121 tgaatgcagc ttctgaagca tcagacgtca tttttggagg aatcggtgga aagctggtca
    27181 ctattgggat cttgatctcg gtatacggtg cattgaacgg ttatacacta actgggatcc
    27241 gcgtgcctta tgcgatggct ttagaagacg agctaccttt cagtaaacag ctgacaaagc
    27301 tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt ccagttagca gtagcttgta
    27361 ttatgatgag ccttggttct ttcgactttt taacagacat gttgattttt gtgatgtggc
    27421 tattcagttt actgattttt atcggtgtct ttattttaag aaaaaaagcg ccggaattgc
    27481 cacgtccata taaagtacca ctttatccaa tcgttccgat tattgcgatt ctcggtgcta
    27541 ttttcatttt aggtatgacg atgatgacgc aaacgaaatt agcattgatc ggtataggtg
    27601 ttaccttgat aggtatccca gtctattatc aaaaaaagaa aaaacgctca gaagagtaaa
    27661 atatttcaac aacagacaga aaaagtacga actaggtcct ctagatcttc gtgctttttc
    27721 tatttcaata aaacaagttc ttctattttg atttttgcta cttctgcaag tagtgcttat
    27781 gtgctaaagt aagaatgaaa aggagagata aacatgatat ttgattctca tacccattta
    27841 aatgctgaac agttcaatga agatattcca gaaacgattc agcgagcaca agaaatgggg
    27901 gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga ttgaaaagtc tttgcaatta
    27961 agccatgact atccaaatat atacagtatt atcggctggc atccgacaga ggctggcagt
    28021 tatacgaaag aaatcgaaaa taaattacaa gaacaattga ctttaccaaa ggttgtagca
    28081 ttaggggaaa ttggcttgga ttatcattgg atggaagacc cgaaagaagt ccaagataaa
    28141 gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc taccaatcag tattcatact
    28201 agagaagcac ttgaagacac gtatcaaatc ctaaaagaag aagatgttcg agatatcggt
    28261 ggaatcatgc atagtttcag tggtgattat gaatgggcaa aacgatttct tgacttaggg
    28321 atgcatattt catttagcgg tgtcgtcact ttcaaaaaag cattagacgt ccaagaggct
    28381 gcagcaaatg taccaatcga acgattactt gtggaaacag atgcccctta tctggcacca
    28441 gttcctttta gaggaaagcg aaatgaacca ggctatactc gttatacagt agaaaagatt
    28501 gctgaacttc ggaatcttcc gacagaagaa gtggctgccc aaacatggac aaatgcgcac
    28561 cgattatttg gtctaagcga acatggataa gatgaagatc caagagatta tagtcgttga
    28621 aggaaaagac gatacaagaa ggctgcggga agtagttgat gcagatacga tagaaacaat
    28681 agggtctgca atcaatgaag agatcctgat gcaaattgaa catgcccaag agactcgtgg
    28741 agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa ataagaaaaa tcattatgga
    28801 agctgttcct caggcaaaac atgctttttt gccgcgcaca caagctaaac caaagaaaaa
    28861 gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt ttagaagcat tgaaaaaggt
    28921 cgtgacgcca gacatttccc aaacggctgt ttctgaaatc actagacaag accttttaga
    28981 ctatggttta atagctgggg cacaagcaaa agaaaaacga gaaaaactag gtgacgagtt
    29041 gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt ttgaagatgt ttcgtattac
    29101 gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa caacatgagt gaatatcgtg
    29161 aaattgcgac accctctaga acaaaagaga ttttattgaa acatggtttt tcatttaaaa
    29221 aaagtttagg acaaaatttt ctgactgaac caaatatttt aaggaaaatc gtagaaactg
    29281 cgcaaataga tgaacatacg aacgtgatcg aagttggacc aggaatcggt gcattgacgg
    29341 aacaattagc caagcatgca aaacaagtcg tagcatttga gatcgatgat cgtttgatcc
    29401 ctgtattggc tgatacgatg caaccctatg acaatgtgac tattgtacat caagatattt
    29461 taaagaccga tttgtcaaca gctgttagag aaacatttca tgaggagctt ccgttaaaag
    29521 tcgtagcgaa tttaccttac tatattacga caccgatcat gatgcatttt cttgaatcag
    29581 atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga agtagctgat cgtatctctg
    29641 ctgaaccagg aacgaaagct tatggttcat tatcgattgc tgttcaatat tatatggaag
    29701 ccagtttagc attcatcgta ccaaaaacag tatttgtccc tcagccaaat gtagattcag
    29761 cgattttgaa actgacaaga agagatatac cggcagtcga agtgacagac gaaaaagcat
    29821 ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa aacgctttgg aataacttgc
    29881 agcattcgta tggcaaagat gaccagacaa aagcatggtt agcaaaaagc ttggaaacag
    29941 ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt acaagaattc gccgcattaa
    30001 gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt gtgacagaag ttgtgaaaaa
    30061 gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat gtttttcgtt
    30121 tgtttgggct tattaccgca gatgcaagct tttgaacacc gtttaagaaa gaggttgtgg
    30181 gcctgccgtt taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt
    30241 gagttagttg gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag
    30301 atgtgacaca aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac
    30361 ttgatactca cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta
    30421 gtaagtgagt tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa
    30481 tgagaaaaaa ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca
    30541 ttatcccaat ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc
    30601 atgtgccatt atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag
    30661 gtacgacatt tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac
    30721 atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa
    30781 aaaatggaca gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg
    30841 tgattcattc tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc
    30901 ctgaaacata ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg
    30961 ggcttatcca cagtttagtt gattataata ttgcttattt tatagcagga acactcagca
    31021 aatattttga tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc
    31081 aagggtacgc gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct
    31141 agtaatggat aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa
    31201 catcttctgg cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt
    31261 tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt
    31321 ttatggaaat ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa
    31381 agcatcgttt cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca
    31441 ggtgaagctt caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata
    31501 acttcttttc cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga
    31561 agcttaagcc catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc
    31621 atggcaggag atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct
    31681 ttcggtgtac cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata
    31741 gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga
    31801 agtttggcaa tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc
    31861 tcatccatta agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg
    31921 cgttgtccac cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc
    31981 tctgctgcat tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag
    32041 ccaaaagcca tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc
    32101 atggcaatat cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt
    32161 tctccttcag agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa
    32221 ccggaaggac caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt
    32281 acagaataat tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc
    32341 ataatacgtt ccacactttc tttttta
//
LOCUS       DACCXW010000018        32185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCXW010000018 DACCXW010000000
VERSION     DACCXW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.018"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(123..503)
                     /gene="yajC"
                     /locus_tag="IVY71_000673"
     CDS             complement(123..503)
                     /gene="yajC"
                     /locus_tag="IVY71_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7984743.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(600..1745)
                     /gene="tgt"
                     /locus_tag="IVY71_000674"
     CDS             complement(600..1745)
                     /gene="tgt"
                     /locus_tag="IVY71_000674"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7984744.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1963..2661
                     /locus_tag="IVY71_000675"
     CDS             1963..2661
                     /locus_tag="IVY71_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7984745.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2829..5468)
                     /locus_tag="IVY71_000676"
     CDS             complement(2829..5468)
                     /locus_tag="IVY71_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7984746.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6087..7118)
                     /gene="queA"
                     /locus_tag="IVY71_000677"
     CDS             complement(6087..7118)
                     /gene="queA"
                     /locus_tag="IVY71_000677"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7984747.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7311..8486
                     /locus_tag="IVY71_000678"
     CDS             7311..8486
                     /locus_tag="IVY71_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7984748.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8490..9131
                     /locus_tag="IVY71_000679"
     CDS             8490..9131
                     /locus_tag="IVY71_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984749.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9128..10045
                     /locus_tag="IVY71_000680"
     CDS             9128..10045
                     /locus_tag="IVY71_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7984750.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10042..10713
                     /locus_tag="IVY71_000681"
     CDS             10042..10713
                     /locus_tag="IVY71_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984751.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11254..11742)
                     /locus_tag="IVY71_000682"
     CDS             complement(11254..11742)
                     /locus_tag="IVY71_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7984752.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11943..12209)
                     /locus_tag="IVY71_000683"
     CDS             complement(11943..12209)
                     /locus_tag="IVY71_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7984753.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12209..12472)
                     /locus_tag="IVY71_000684"
     CDS             complement(12209..12472)
                     /locus_tag="IVY71_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7984754.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12465..12737)
                     /locus_tag="IVY71_000685"
     CDS             complement(12465..12737)
                     /locus_tag="IVY71_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984755.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12948..13133
                     /locus_tag="IVY71_000686"
     CDS             12948..13133
                     /locus_tag="IVY71_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7984756.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13275..15098)
                     /locus_tag="IVY71_000687"
     CDS             complement(13275..15098)
                     /locus_tag="IVY71_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7984757.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15696..18401)
                     /locus_tag="IVY71_000688"
     CDS             complement(15696..18401)
                     /locus_tag="IVY71_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7984758.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18707..19654)
                     /locus_tag="IVY71_000689"
     CDS             complement(18707..19654)
                     /locus_tag="IVY71_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7984759.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19844..20113
                     /gene="rpsN"
                     /locus_tag="IVY71_000690"
     CDS             19844..20113
                     /gene="rpsN"
                     /locus_tag="IVY71_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7984760.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20289..20651)
                     /locus_tag="IVY71_000691"
     CDS             complement(20289..20651)
                     /locus_tag="IVY71_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7984761.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20662..21783)
                     /locus_tag="IVY71_000692"
     CDS             complement(20662..21783)
                     /locus_tag="IVY71_000692"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7984762.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21799..22149)
                     /locus_tag="IVY71_000693"
     CDS             complement(21799..22149)
                     /locus_tag="IVY71_000693"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7984763.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22279..23790)
                     /locus_tag="IVY71_000694"
     CDS             complement(22279..23790)
                     /locus_tag="IVY71_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7984764.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24112..25464)
                     /locus_tag="IVY71_000695"
     CDS             complement(24112..25464)
                     /locus_tag="IVY71_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7984765.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25617..26693)
                     /locus_tag="IVY71_000696"
     CDS             complement(25617..26693)
                     /locus_tag="IVY71_000696"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7984766.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26889..28634
                     /locus_tag="IVY71_000697"
     CDS             26889..28634
                     /locus_tag="IVY71_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7984767.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28914..29351)
                     /locus_tag="IVY71_000698"
     CDS             complement(28914..29351)
                     /locus_tag="IVY71_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7984768.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29335..30708)
                     /locus_tag="IVY71_000699"
     CDS             complement(29335..30708)
                     /locus_tag="IVY71_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984769.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31030..31518)
                     /locus_tag="IVY71_000700"
     CDS             complement(31030..31518)
                     /locus_tag="IVY71_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7984770.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31540..32142)
                     /locus_tag="IVY71_000701"
     CDS             complement(31540..32142)
                     /locus_tag="IVY71_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7984771.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9200 a         6884 c         5942 g        10159 t
ORIGIN      
        1 tgacacaccc gattattcaa tattgagcaa tatatcccat gctcttatct ctttcttttt
       61 tgaataaaaa atgaggtagg acatctttct gccctacctc gtctttaaat aagctttgat
      121 acttattttt cttccttctc ttctgtctct tccagcgttt ctgttgtttc agtttttact
      181 tcaactgtct cagctggttc ttctgttgtt tcaacagtag tcgttgcatc atttgttacg
      241 acagtccctg gtttgaccgt tttgattgct gcacgatcgt actcaaggaa gatcccttcg
      301 cagtctaaaa caactgtccg tttgtcattg tcgatttctg aaacaactcc atgtaatccg
      361 ccgatcgtta cgactgaatc acctggtttc atgctatcta acaagttttg acgttcttgc
      421 tgttgtttct tttgcgaacg tgacataaaa aaccacattg ctaatagcgc gataaacatc
      481 acaagcattg gtaatgttcc catttttttc acctcactaa ttctgtatct acaaaaaaca
      541 ctttagcaga aaaatgctga taacactagt tttttactta atttttaaaa gattttcctt
      601 tagaagcttt ttgcgttttc tttattgaac ccgtattctt caaaaaaggc ttctctaaat
      661 tcaagcaaat tatcatccat gattgcttga cgtacttgtt tcattagatt caacaagaag
      721 tataaattgt ggtaagaagt caaacgaatc ccaaatgttt catcacattt gatcaaatgg
      781 cggatatagg cacgtgtata gtttttacat gtataacaat cacatttctc atcaattgga
      841 cggaaatcat gggcatattt cgcatttttc actactagac ggccttgaga agtcatacat
      901 gtgccatttc gagcgattcg agtaggcaag acacagtcaa acatgtctac tccacggata
      961 acaccatcga tcaatgaatc agcagcaccg acacccatca agtaacgtgg tttgttttcg
     1021 gggatcaacg gtgtagtgaa ttctaataca cgattcattt ctgatttagg ttccccgaca
     1081 gataacccgc cgattgagta accagggaaa tccatcccga ctagatcttt cgcgctttga
     1141 cgacgaagat cttcaaaccc tgctccttgg atgattccaa ataatccttg acggtctgga
     1201 ttagcatgag ctttcaatcc tcgttctgcc catcgagacg tacgttctac tgattttttc
     1261 acgtagtcgt aactttcatc gaatggtgga cattcgtcaa aactcatcat gatatcagag
     1321 cccaatttat tttggatgtt gattgctttt tctggtgata aaaacatacg cgaaccgttc
     1381 aagtgatttt tgaaatgcac gccttcttct tcgatattcc gcatatcact taatgaaaaa
     1441 acttgaaagc cgcctgaatc tgttaaaatc ggttgatccc agttcataaa tttatgcagt
     1501 ccgccagctt cttctactaa gtcttcacca gggcgtagcc ataaatgata tgtattactt
     1561 aaaataacgc cagcacccat ttctttcaac tcttctgggg acattgtttt tactgtagct
     1621 aatgttccta ctggcatgaa cattggtgtg gggaaagttc cgtgtggcgt gatcaattcc
     1681 cctaaacgcg cgccagtatg tttttctttc ttgattaaac gatagcgaat ggcaggttgc
     1741 gtcattcatt tccctacttt ctatataaaa tatcttgaca actcccacca tcataatcgt
     1801 ttcaagtgct ttttgcaagt tccctcggct tccttctact ttatttaata ttcttttttt
     1861 tatccttttt ttatgaataa caaaaaaaag aaaaacatta tgacagaaat attttttgat
     1921 acatggtata ttggtgttat taaagaaaag gagggaaaaa tcatgcaatc ttctgcagaa
     1981 ttaaaacgcc aagcaaaaga gagtctaaaa ggacgttggg gacaagctgt tttactaaat
     2041 ctaatcccta cattgattgg tattgctact attcttctta ttatgattcc tattatcttg
     2101 tttgccacca ccatatatga tggatcagcg agcgtctcag aagcgacaac ttcaggaggc
     2161 ggtagttcag gaggcggtgg cggaattatt tccagcatca ttagcgcttt gttcatgagc
     2221 ggtatttctt ggacttatct tgatatttta cgtggacaaa aagatcagat cgaaccattc
     2281 aaagatgcat ttcgtggatt caaaggtttg tttatcggcg gagtgatcag cttagctgtg
     2341 ctgatctcga tctttacaac attatggggt ttgctattag ttattcctgg aatcattaaa
     2401 agctattctt attctcaaag ctattttatt tactacgata tcgtccaaga aacaggacaa
     2461 acacctaaag tactggatac gatcactgca agtcgaaaat tgatggatgg ttataaagga
     2521 caacttttct ggttagatct ttcatttatc ggctggcata tcttagcatt agcaactgct
     2581 ggtattggtt atctatggct cagcccatac atttctgcaa caaaagcagc attttatgaa
     2641 caattgccaa aagatattta aaagagagtg tgacttgtga ccgagggggt ggcaggagaa
     2701 caccgtttat cgagaggcag taaaagaaac attcagccca ttagcaaaaa ggaggtgtga
     2761 caacattcat gtcacacctc ctttttgtat agttccagta aaaagaaaat caacttagaa
     2821 acagttaatc attcctcttc ttctgcttcc ggtaatcgat ggaacttgat cagttcattc
     2881 accgcgattg gtacgataga taaaatccct accaagaacc agtgattcaa actgatgtgt
     2941 actaggccga atagttcttg cgtaaatggc agcaaagcaa cagccgttgt aatagctaat
     3001 gccagtaacg ccatttctac caaagcttta ttggtcgcta aattgacttt gaagatcgat
     3061 tggctgctgc gtacattgaa cacatgcaga atcgatgaat aagctaagat caagaatgca
     3121 acagtttgac cgacatgctg acttgcgtct acccatgggc tgatagtatc gacataacta
     3181 cctaaataat aaccaaacaa ggttacgatg gtgaagaccc ctgcatttat ggcaattttt
     3241 tgcaataaac ctcgagcaaa gatcccttca tttttcggaa tgggtggctg atccatgatg
     3301 ccgtgttccg ctggctctct tcctaaagca aatcctggaa gtccatcagc aactacattg
     3361 acaaacagta gttgcacagc tgtaaatggt gctccccatc ctaacagcat cgcaatcaaa
     3421 acaataaaga tctccgaaat attacagctt agcaaaaagt tgatagcttt acgaatattc
     3481 tgataaaccg aacgtccttg actgacagcg tctacgatcg tcgcaaagtt gtcatccgtc
     3541 aaaatcatat ctgcggcact ttgcgccaca tctgttcctg tgatccccat ggcacaccca
     3601 acgttacttg ctttcaacgc tggtgcatca ttcacaccat ccccagtcat cgctacgact
     3661 gcacccgtac gttgccaaga tttgacgatc cggatcttgt cttctggcgt tacccgagca
     3721 tagacagaga gagctttgac acgcgcatct aattcttcgt ctgataattg atgcagttct
     3781 gatcctgaaa gtgcttctga cggatcattc aagataccta attctttcgc tatggcactg
     3841 gcagtcacca catgatctcc agtgatcatg actgttttga tgccagcctt tttcgctcgc
     3901 ttgattgctc ctttactttc tggacgtggt ggatcgatca ttccgatcag accaatcaaa
     3961 cgtaagtctt tttccagtgc ttcagaagaa atatcttgcg gttcttcgtc ataagtagca
     4021 taaccaacag caatcactct cagtgcccgt ttaccaaagc gatcattaac gatcgccgct
     4081 tgttctacat cgcctgaact aaagcgcgga gcaagaacat cgaatgctcc tttggtgatt
     4141 gagatgtatt tttcgcccat ttggtgaact gttgtcatca tttttcgttc agaatcaaaa
     4201 ggaagctcgt ttactcgtgg atatttttct tctaattctg cctttgtatg ataattctct
     4261 tcaacagccc ttacgatggc tacttctgtt ggattacctt tgataacaag gtcgtcatct
     4321 tcttgctcga ccgtcacatc tgtacatagt gcagccattt tcaatatttc catcgcgctg
     4381 ttcgtcattg aatcttcgat attcgttact tcatcttctt tgctccatac ccggcggacg
     4441 cgcatttcat tttgtgtcag cgttcctgtt ttgtctgagc agataacatt agcggttcct
     4501 agcgtttcaa ctgctggcaa ctgtcggatg atcgcatgtt tcctcgccat cttctgcaca
     4561 ccgtatgcaa gtgtcaacgt aacaataaca gtcaaggttt ccggtacagc tgcaacagcc
     4621 aaggaaatcg aagtcatgaa catctcaagg agtggttctc cttgcagttc accaatcaca
     4681 aatactaaag ccgcagccgc taaagcgatc atactgattc gtttgcctag ctgattcaat
     4741 cggacctgta aaggtgtttt ttgcatagta ttttcgttca aaagatccgc gatcttaccc
     4801 atttctgtag ccatgccaat ggcagtgacg atcgctttcc cacgacctgc agcaaccgta
     4861 catcctttaa ataccatatt aagttggtca cctaatgact catcttcttc agcaataaat
     4921 gctgcatctt tttcaactgc ttcactttct ccagtcaacg ccgattcttc tacacgtaat
     4981 tgtgatgctt cgatcaaacg ggcatcggca gaaatagcat caccggactc taaaacaaga
     5041 atatcaccca ctaccagttg ctctgactca acagtggtca ctttgccgtc tcgtatgaca
     5101 gttgccgttt gtttattcat atcttgcaag gattccagtg ctttttctgc gtttccttct
     5161 tggacaatcg ccaataccga gttgatcaca acgatagcga tgatcagcag tccttcaaat
     5221 aaatccccat gttctgtagc aaatgcggta taaaaggaaa tcaccgctgc tacaagaagg
     5281 atgatcgttg taaaatcgga caagcttcga aagaattttt tgatcatcga ttctttcggc
     5341 gcctcatcga atttattcaa tccctgcttt tttcgccgtt catccacttc ttgtgaagac
     5401 aacccagtat ctggatctgc ttctgttacc ttctttactt cttctattgt ctgcttgtat
     5461 tcttccatcc tattcctcct ttaaaatctt cgtacaaaaa aaggtactta ttttactata
     5521 acatcctttt tttatttact tcaaataaag ggcttatcga ttatttgtta agaaagcgta
     5581 aaatttaaag tgcttatatt tgcaaacgct accaaaggat gctattatat agatgtaagg
     5641 aaggaggata ccatcaaata gacagaagaa tttctcgata gaacagtgta agcaaacaaa
     5701 gttgttagaa gtacaggttt cacccagaca tgcgtgtaga gtacaaacct ataaatagtg
     5761 aatgcgtatg gatgtttgga tcacgaagta tccgaaacaa gaagtaatgc gatacgtaac
     5821 aattgaataa cgtaaacgtc tgaaaattga ataggaacaa gagatgtttc aaatgttgaa
     5881 gatgtaacga aaacaaaatt tttgtttaac atgaagaaat tcaacaccgt gctttgcatg
     5941 tatgatggaa agttacaatc atttctgttt atttgaatcc ttccgaggta caagaaagcc
     6001 gagcttatcg agagctcggc tttttctttg tcataaaatc tttgtaaagc ttttttgtta
     6061 ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag
     6121 aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca
     6181 aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct
     6241 tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct
     6301 ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg
     6361 acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc cgcttcttcc
     6421 gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt
     6481 cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg
     6541 atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca
     6601 ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat
     6661 ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc ttcatactca
     6721 aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctactgcttg caatctgcca
     6781 tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggtttgat caatgtctcc
     6841 cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt
     6901 ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca
     6961 ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc agttttccga
     7021 tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gattaattct
     7081 tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat
     7141 gtgcttcatc attctatcat gtttttcgtt ctgttggcaa aatcagcact ttttcacttc
     7201 tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc
     7261 atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat
     7321 ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt
     7381 catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga
     7441 cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa
     7501 aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa
     7561 gtattggatt gatgcctcac atgacgatcc gtgaaaatat cactcttgtc caaaaattgt
     7621 tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat
     7681 tgccccgtga gatgttagat cgctacccac atgaattatc tggtggtcag cagcagcgta
     7741 ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg
     7801 gtgcgcttga tccaatcact cgtgattcct tgcaagacct tgtcaaagat ttacaagagc
     7861 gtttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aaactagcaa
     7921 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatattt
     7981 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
     8041 ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc acgattacac
     8101 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
     8161 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
     8221 aacgaggaaa agtatccagt gtcggcgaca ttttgaataa acatgttttt tacgtaaaag
     8281 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcgggctg aaatacgtgc
     8341 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
     8401 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagcta
     8461 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
     8521 aaattctctc aaaaggtttt gaacatcttt atatttcagg acttgctttg atcattggta
     8581 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
     8641 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
     8701 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
     8761 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
     8821 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
     8881 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
     8941 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
     9001 attaccaacc agatctcatt ttggcaggta cgatccctgt aacaatttta gcactattgg
     9061 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
     9121 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
     9181 gagttgttcc ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
     9241 tggaatcact tcagaagcac agatcttagc aagtctagtt gccggaatgg ttgaacatta
     9301 tacagataaa aataccgcaa tcatcaacaa tctggctact acaactatca accatcaagc
     9361 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
     9421 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
     9481 atttgagaaa agatacggtc aaacgtggtt cccgtcctat agttttgaaa atacgtatgt
     9541 ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca gcgatctgaa
     9601 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
     9661 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
     9721 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
     9781 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
     9841 cccaccatat gatgctgctc cagttgtaga caacaagata ttagctgata cgcctcattt
     9901 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
     9961 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
    10021 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
    10081 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
    10141 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
    10201 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
    10261 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
    10321 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
    10381 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
    10441 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
    10501 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
    10561 ggagatatca ttatccgagg aaccaatgca acagagggca catcgattat tttagcaggt
    10621 gcattgccaa ctgcattaat ggcgatcatc acggactggc ttttaggtat tttggaaaga
    10681 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
    10741 cgaccatttg ttggtcgcaa aggctttttt agttctgtca tttttaaggc aataatccac
    10801 tctgattctt ccctccttat gggcaaagag ttgcttttct tagctattta tccattccgt
    10861 atactccaac ttcatactgc caacgggttc atatctgcat ccaccgacta agtttttttc
    10921 ttatccgcta aggcattttg ccaatccatt ttcccatcac tcgtcatgga attcatacca
    10981 catgatctct aaatactcgt ctgcttgttc tttgaattgg gcatcaattc ttacatcctt
    11041 tggcgtggct ttcatttaag ccttttccat cagtcctcga ttgttcgatg ccttcagctg
    11101 catcatcaaa actggtgact gccgtatcgg taacttcgat catcttttat acaatcggga
    11161 acttgccatc ctcatccttg gctaaaaaca tatctagcca ttccagcttc tcgccatggg
    11221 aggtttgcgg ttcctaaaac cggtacacga ttcttatgga ctgcatccgt cgcaactaga
    11281 cttggcgata cgatgatcct tcaccagaag agcacccccg taaaccaatt gatcgatata
    11341 ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg cattgccgtt
    11401 gatactgcta ttcatggccg aaagcgccac gactttaact tcttccgttt gagtgacatt
    11461 catgttcggc aatgtcgctt tggatacccg ctctgctaaa ggaacgacac tggtattgta
    11521 tttcgtatct gtatcttttc caaaagacca tgccaacgaa tcctctggga accaataaga
    11581 cgactccggt tgattgtcca ttgctttttt caacatactt ttggctgttt ctcgataaac
    11641 gacatttcca attgtcttcg cctcactttg gcgataatcg atggtcgttg ccactgccaa
    11701 cactaacaac gatgctccaa ttttactgat tttccttgtc acacttcttc ctccttgttt
    11761 tacataaacc cgtcaacatt ccattcctaa actcaaacaa tgacccttca aaggtggaga
    11821 tataggaaca aattaaagaa atcatgcaat aaatccaaga aggttggttt tagaacaaaa
    11881 aaagagtctc ctttttgggg agactctttg ttaactatct aaaaaggttg gctcttctca
    11941 cttcactcat aatgtgtcca ggcagaataa atttctacaa ctttattttc ctcatcaact
    12001 ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt atctgccttc atgtgtaggt
    12061 ctcagctttt caaaagactg cgtgggcata tatggatctt ccttcaatgt ctgaatcact
    12121 tcttcaaatt gtttctttaa attcgtttgc ttgatttttc ttaaatccac tttcgcagag
    12181 tttttgattg cgactgtata gttactcatc aaagagcttc ccaatcaata tcatcgatat
    12241 tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt atctaaagta cctgtttgct
    12301 caagaaaaag ggtttcttga atcgctctcc agtcttccag cccaatcaat acagcattgt
    12361 ttccactacg atcactaata atctcgattt cagtatgatt ttcatttacc tctttcaata
    12421 actgataaaa atttttacga gcagtgctag gattcatcgt tgtattagcc attgtctctc
    12481 ctcctttttc tgatataatt atagcgtact tgattaagta cgtcaaaaaa atacaataaa
    12541 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
    12601 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
    12661 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
    12721 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
    12781 acctgaggat acgcacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
    12841 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gtcattcatg ttcactatcg
    12901 atttgatact tgcttagacc gttgggtttg tgaatcacca attaaaaatg gcactgagtt
    12961 atggtaacaa aaaatggtca cttgacgagg ctgagattcc tacttatgaa gacacatggc
    13021 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
    13081 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
    13141 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
    13201 tgagaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
    13261 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
    13321 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
    13381 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
    13441 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
    13501 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
    13561 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
    13621 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
    13681 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
    13741 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
    13801 aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg caatgattgc atatgagatg
    13861 aacgcacccg taatattggc aatcgatttg cttaaccatc tttttccttc ttttttccat
    13921 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
    13981 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
    14041 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
    14101 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
    14161 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
    14221 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
    14281 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
    14341 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
    14401 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
    14461 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
    14521 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
    14581 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
    14641 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
    14701 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
    14761 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
    14821 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
    14881 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
    14941 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
    15001 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
    15061 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
    15121 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
    15181 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
    15241 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
    15301 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
    15361 atcttaactt ttggaagaga gatgtaaggc tgctttctac ccatcattct tactactcga
    15421 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
    15481 aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
    15541 tgatgcagag cagctttttt acaacctctt ggtacacggt gttcaatcag ctgaccctcg
    15601 gtattagctc aaaaaaacgg caaaatatga ggaactattt ttccattttt ttctctaata
    15661 ctcaggtcaa aacactgatt tcacaacctc tttttttaat gaaaaatatg gctctcatgc
    15721 gccgcttctt tgaatttcgt cattttttct tcgatgaacg gatagattgc cgttcctacg
    15781 attccaaaga caacaaacaa accgatcatg atccagattg ctttcgtagc atttggccaa
    15841 taaattcctc ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg caagaacgga
    15901 ttgatcattt ggaagaattt ccctgagact tggatcggat agttcccgcc tccgccagag
    15961 atcgacagta ctaagatgat aatagcgatc cctttaccga cattatcgaa taatgcagcc
    16021 aagacataga cgatcatcat gaacgctaag gcgatcaaga cagcaaatga cacgctgtag
    16081 accggatttt ggacatctac tcctagtaag aaaatattcc ctaatgtaac gatcaagctt
    16141 tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta
    16201 aaattgtttc ggtctttctt tcctaaatag tattctgtcg tagcaacact tgaaagaaga
    16261 acagcgccga cccacagaca tagtgcagta taaaacgggg tgctagctga accgttattt
    16321 gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct
    16381 tttgttgcgt ctagtttcaa taatttgatg acttcgccta gatcgacttc ttgttcgcct
    16441 tttcggatcg cgtctgctgc tttttggata ccagctttga tatttggcca gtcattgttg
    16501 atcaagtccg cagcagcgtt cacagctgtt tcgacttctg gcattttttc atttactgtt
    16561 gtcaatgtac cggtgatttc attttttagt gctggccaat cattttggat aaaatcagcg
    16621 gccaatccta atttttgctg aatgatcggt aattcattgt tgtataggtc tgccccttca
    16681 ttgatgccat tgacgatggt atccatattt ccattcagca ttgtattggc atcatggatt
    16741 tcttgtccaa ttgcaggcat ttctttttga tatttttcta aaattgccac tgcattcgtt
    16801 acagttgctt tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc tttgctcagt
    16861 gcgccttgtg cagtcccaat cgtatcgatg acttggtcta gtagagaagt gacagtggaa
    16921 ctgatttgac ctgtattgat accagcaatg atgccattga tattattaac aacggcatca
    16981 atttgatcta gcaattcatc aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa
    17041 gcagataggc ggttttgcag attgcttagc aagggtttta attgatttag ctgatcaata
    17101 attggctgta atttcgtatt tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt
    17161 ttatcgattg cattttgttg ttgagatatt ccgtcaataa agccgttcaa tacattgctg
    17221 atgtttttct tttcatcatc agtcagctta ccatcttcaa tagctgttct tacagtatct
    17281 attaacgaac ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac atctacactg
    17341 tttgtgatgc ttggtatcgc atctttcagc tgagaggcac ctgattttgt ttggtttgct
    17401 agatcacttg cctgattttc taaatttttc acatctggca agatatcttg tacttgttgg
    17461 atgatcgtca agccttgttt tgcttcatcg attccttggt tcattgtctc ttggatcgaa
    17521 gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat tttggatctc tgggattttt
    17581 tcttgcaaat caaggatgat ttttgcttga tctttcaact caggcatttt gtcattcaat
    17641 gcaacgactt tttcacccat ctcatctact ttagggatgt aatccacgaa ttcatttgct
    17701 ttattcagtt tttccttgat ttcaggtagt tgactttgta attcgaggac ttgttttgtg
    17761 tagccgtcga tcgtgtccaa attttcatct gttgataaaa tcatatcttt aactttattg
    17821 atacttacta ggttcgtatc gatgtcgtaa cctatctcat tgaatacttt caataaagtc
    17881 gaactggctg ttttgataaa ttcatttgtg atttgcgatt ggatcgaact tgctccttta
    17941 tcggtgattt tcggtgcaat cgcattgatt ttttcattta ccgtatattc gatttttggt
    18001 ttcttgatat caccactagt aaaactcagc agatcttctg agaaatcctt tggcaagtaa
    18061 atacctgcgt aatactttcc ggaacggact ccgtcttcta attgttcttt tgaatcaacg
    18121 aattgccatc ctaattgttt atttttatga agcgattcga tgacttgctc cccaatggcc
    18181 acttctttac cttgaaactc tgctggcttg tctgcactgt aaacagcaat tggcagctct
    18241 ccagtatttc catatggatc ccataatgct ttgatgttaa accacgcata tagcgaaggg
    18301 atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag
    18361 tcaagcaaat ataatcgaag cgtatttttg attgactgca tagttctccc ccttcttctt
    18421 ttttcagctg aattacttta ccatgtattt catacttttt tcaatcggct tttttcaaaa
    18481 aagtcgaatt tttggacata tcacggcaat tgtctcattg taatctctta catattgaga
    18541 aataattaag gattacagcc tttactttat catgaaataa aagggaaaga taattttatg
    18601 ccaaagtttt tatctattat acgtatacgc aaaaaagcta aaacattttt tgtctttgcc
    18661 cctacaaaaa tgtttcagct tttttcgttt tttattcctg tatttcttat ttttcgaatg
    18721 acaggatgat tttcccagca ggaggatgtc cttcaactag ctgatgtgct tggatgacat
    18781 gcttaagtga agcagacaga ttcattgaaa taaaaacatg aaaggctcct ttttggtagg
    18841 ctttgattat gccagcaaag gcttctgcat ctagatattc ctttcttgga acaaacgatt
    18901 cataaaagcc ttcctttttt tgccgcaaat ctagatctgg gaggtcattt aatgcaacat
    18961 acctgccgcc tggtttcatg atctggatgc cggtttctcc tttgatactt cctttcgtcg
    19021 catcgattac aaggtctgcc tgatcagcaa attgcagacc tggatttgtt ttatcataag
    19081 cagcaaatgc tgaagctcct aattttttta ccttttcctc atttttactg gatgcactgg
    19141 ttaagattcg gattcccttt tcatggagaa gctggatcag acttgacccg accgcacctg
    19201 atgctcctag tatcattaca gtatctgtcg gctggatttc cgtcaaatgg ttgattagat
    19261 tataagccgt aattcctggc gtgaccattc cggctgcttc ttcccaactc attttatctg
    19321 gaatttttgc tacttttgct gatggcaaca cgacttcttc tccgtaagtc ccgccgactg
    19381 catggacagc tactcgatca cctacatgaa cagttgtgac atctgaacca atctctgtca
    19441 caataccagc gccatcatta cctggtacat aagggaattt caatgttcgt atctctttca
    19501 tttctcctaa acgcaaggca acatcataag gattcacact gaatgctttt atttctacac
    19561 gaacatgtcc agaaccgact tctctgggat gagcatcgat ttcttcaaaa acatcttgtg
    19621 cttctccgta accgttaatt gcaaatcttc gcatggtcac catctccctt tttctttaag
    19681 tttacgttta tgcctttatt ttgacaaaaa aaatgtcttt tcgttttggc gcatgattca
    19741 aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa aagcgtaaag
    19801 attacgattt acgctaataa aaaataattg gaggaatttg ttgatggcta aaaaatcaaa
    19861 gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt tacgccagga
    19921 attaaaagcg caaaaagatt acgaaggctt gcgtaaactt cctttagatg ctcgtccaga
    19981 acgattgaaa aatcgtgatt taatcgatgg acgccctcgc ggatacatgc gtaaattcgg
    20041 gatgtcccgt ataaaattcc gtgaattagc acataaaggt ttgattccag gagtaaaaaa
    20101 agccagctgg taatccaagt ctggctgtaa tgtaaaggag gagggcgcat ggaccactcc
    20161 ttggatcatc aaaaaagata cgttcgcttc ttctagcaga aatgctggta ctagaagcag
    20221 acgtatcttt ttttacgcac agctggtcct tttttatatg taaaggctgc atggttactt
    20281 cgctatattt atttagtaac aggagtcagt gcttcgatac ccacactaat tttcaatgct
    20341 cggtcaactt ctgccataat gggtcgatcc aaatggcaga ccttttcttt caatcttgat
    20401 ttatcgatcg ttctgatctg ctccaacagg ataacggagt ctctttctat tcctgttcca
    20461 gtagctttga tcccaatgtg agtcggaagc ttaggtttag ccatttttgc cgttatggcc
    20521 gcaacaatga tcgtcgggct aaaatggtta cctagattat tttgaataac caaaactggg
    20581 cgtgttccgc cttgctccga tcccacgact ggagaaagat cagcaaaata aatatctcct
    20641 ctttttacca taggatcctc cttattcttg atattctctt ggtatccgct gtcctaatcc
    20701 gcaagccact tcatagtgga tcgtacctaa ttgatctgct atatcctgca tagtgatttc
    20761 tttgtctgca tttttgccga taagcgtcac ttttgttccg acaggaaatt cttgaggcaa
    20821 tcggatcatc agttgatcca tacatacacg cccaatcgtt tcacagtagt tcccttctac
    20881 caatagagaa aaaccctgca tctttcgtgg ccagccatct gcataaccga tcggtatcgt
    20941 tccgatccac tctgcttcag gagtgatata tgtttctcca tagccgattc cttcacctgc
    21001 tgggagtttt ttgacttgaa tcaattcaga aaccagctca agtgctggct gcaatggata
    21061 aacttccggt agtgcgtgcc cagagggatt aagtccgtac attgctactc cataacgaat
    21121 catattgccg attgtttcat catgccataa agccgttgca ctattgctga catgtacata
    21181 tcgaggaagt tcgggaagtt ttttcagcac ttctataaag cgttcttttt gcagattcca
    21241 atatgtatca tctgcctgat ctgctgtaga aaaatgggta aataaacctt cccattctaa
    21301 tacacgactt tctttgatga atgcagctgc ctcttttact tcttcaggtg tacaaaatcc
    21361 gatacgaccc atcccagtgt ctgcttttag atgaatcgaa agaggcgtct ctagcttact
    21421 tttctctaaa acagcagctg ctgcttgcaa ccattcccgt gtacctgcag ttactgataa
    21481 atcatgagca acagctacag tcagtgcgtc gtaaggaacc atattcaaga taaggatggg
    21541 ctgagtaaat ccagcttctc tcaactcgac tccttcatct aaatttgaaa cacaaaagcc
    21601 cgaagctcca cctgcaactg ctgcctcagc tgtttcaata gctccatgac cgtatccgtt
    21661 cgcttttact actgcaaaca attcttttcc ttgcggaagt cttgcaactt catttgctac
    21721 attttcagtt atagcttttt tatgtatcac tgctttcgtt gggcggtgcc aagcgacaac
    21781 catcgttttt ccttctttct agtcttccaa aataatttgt gcaaaagctg tttcatttgt
    21841 atgagtaata ctgacccata cattgccttc atgaggtgaa cgcgtcacaa caggctgacc
    21901 attttcattt tttaaaatct cgacatcttg aaaggtaact tttccgatcc cagttcccca
    21961 agctttagag aaagcctctt tgcaagcgta acgcccgcct aaaaattcta cttggcgatg
    22021 aaaaggcaag gactggaata gtttcatttc atcagaggtc aagattcgtg caatgaattt
    22081 tggtttttct tcgatcagcc gagtgatcct aggaagttcg actgcatcga tcccaattcc
    22141 ttttatcata tgcttttctc ttttcaataa atttagaaca ctctcattct accaaattat
    22201 aaccattttg ccacaaataa gtccacaaat aacaaaaatg aaagtttctc ttaaaaaaga
    22261 gaaactttca ttttatgttt aatcgttatt tgaacggatt acaaatccgc gttttttatc
    22321 attgtgacgt ttaccgccat ctttttggct gttattattt ttgctataac cgccttttga
    22381 tttgtttcca cgataattac cgccgtcacg acgattacgg ttgtttccgc caccacgttt
    22441 gccattttta ttgtatcctt ttttctgcat tggcaatggg cgttctggtg tgatcttcac
    22501 tggcactgca tcagctggat cttttgctgt cgtttttagc aaaagtgcaa ctaaatcttg
    22561 tgcagaatat tcttctaata atttatctgc tgtttgcagg tatttatcta gaccattttc
    22621 tgcaagtttt gtttcgattt gttcaactgc agcacctaat tgacctttga atgcttcttt
    22681 ttcggttggt ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa tcacatgcaa
    22741 gtagtccatt tcatttggtg taacgaaagt cacagacata ccgccttttc cggcacggcc
    22801 tgtacgtccg atacggtgaa cgtagctttc tggatcttga gggatatcat agttgtacac
    22861 atgagtgaca cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta caaggatatc
    22921 taaatgaccg ctcttaaatg aacgcaatac gctcattcgt ttttgttgag acaaatcgcc
    22981 atggattcct tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt cgtctacacg
    23041 gcgttttgta cgtccgaaga cgattgttaa ttcaggtgtt tgaacatcaa acaagcgagt
    23101 catgatatcg aatttttcgt attcttttgc gcggacatag tattgatcga tcaagtcagc
    23161 tgtcatttct ttcgctttga tcttcacatg atgtggattt ttcatgaatt taacgccaat
    23221 attcttgata gctggcggca tcgttgctga gaaaagtaat gtttgtcttt gatcaggaac
    23281 ttgtgaaatg attttttcga tatcttccaa gaagcccata ttcaacattt catctgcttc
    23341 atccaaaact aatgtttgaa cggttccaag tttcaatgtg tggcggttaa tgtggtcaag
    23401 catacgtcct ggtgtaccca caacgatatg tggacgatct ttcaagccac ggatctgacg
    23461 gccaatatct gctccgccgt aaacggcttg tacacgtact tttttgtcgc gccctaaacg
    23521 atataattct tcttgtgttt ggatagctaa ttcacgtgtt ggtgcgataa ctaaaccttg
    23581 taattcgtga cgatctggat cgattttttc taacattggt aatccaaatg cagctgtttt
    23641 ccctgtacct gtctgtgctt gtccaataac gtcttttcca gctaatgcta gtggaatcgt
    23701 ttctgcttgg ataggcgttg cttcttcaaa accagcgcgt tcgactgatt tcaacaattc
    23761 tggtgataat tctagttctt taaatttcaa atgaatcctc ctatatggtt gttaactgag
    23821 gatgtagctt ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt ttctttttga
    23881 cagtggccac gcacgttttt atccattctt agcccctgtg gcaccaccga aaaatcactt
    23941 tgcgaagttt aggcattaga aaagccaaac gggtcgcgtt cagctttatc attattatga
    24001 catctggctt caacaagttg tttctatggc aaaaccagtc caatttctct tcatctttcg
    24061 gcaataggtt attctaacat agtttttcgt tttctgcaag tttacagatt tttatttcat
    24121 ttgctgaagt tgttctacca cttcaaataa gcccatgctg ttgctgcctt ttagcataat
    24181 actatctgtt gcttcaagat tttgtttgat agcatcaatc aatttattct tttctttttc
    24241 aaaatagaac acaggtagtt gggggtattt ttggttcaat tcttctttta gtgcatgcat
    24301 ttgttcccca taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt cactcatttg
    24361 cgcatgcata tttaaagact ctttccctaa ttccagcata tctgctaaca cagcgattct
    24421 gcgtcctttt gttggcagat ttttaaaagt atctaatacc agtcccatgg ctgttggatt
    24481 tgcattgtag acatcgctca aaatagctgc accatttcct gccgttagcc attcggttct
    24541 gttttgagtg atagaaacat gggcgagccc ttggcgtatc tcaggtatgg acagtccaaa
    24601 gaagcgcccg atcgtatacg caatcaaagc attcgttaca ttataacttc caggcagcgg
    24661 gatcgtaaat gtttctcctt ctactacaaa atcggtttgt gcttttcctt ttgctgtcac
    24721 ttccgcatga acatcgccat ttccaatacc gaatgttgtg actgtttgta ctaatgttgc
    24781 cgttaaaggt gtcagcaacg gttcatctgc gggcacaacg agtaatccgt ctgatacaag
    24841 tccgtcagtg atttccattt tggctttagc gattccttct cgcgacccaa gattttctat
    24901 gtgtgcttca ccgatcattg tgattgctgc agcttttggt tgagccataa gtgaaagttc
    24961 cgtgatttca ttggcgtgat ccatgcccat ttccaaaacg atcttttctg tatcttgggg
    25021 catgtgcaaa atcgtgtaag gaagaccaat attattgttg taatttcctt gcgtcttata
    25081 tgttttgtat ttttcagaca agacagacgc ggttagatct ttcgtggtgg ttttaccatt
    25141 gcttcctgta attgcaacga cttctgcgcc tactttttcc aagtaatagg ttgccaattg
    25201 ctgcatggct ttcaaaggat ctgctacagg aataactgga aaattttttg gagcttgttc
    25261 aacaggcaag ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct ggataaattc
    25321 atgtccatct cgcgtaccag caagaggaac gaataaactt ccttcggtta tcttccggct
    25381 gtcaaattcg acattggtaa tctttgtcag tgcagagcat tcgctgccca cggcttgtgc
    25441 aatttcttgt attgttagat tcattgagat ctctcctaaa aaaggttgtg aaaaaagcgt
    25501 ttggcctaaa cggcgggttc gcatgactct cacttccaag taaatttctc tattcgtatt
    25561 tagaagctag aatgaacgcg gttacgcaat cactccagcc tcttctttat ttctctttat
    25621 tcattttttt caaaaaaaga ttgacgctga tggtatcgat tcattcctaa ctggatcagt
    25681 tcttcaatca aatcaccgta tttcaagccc atattttccc ataagagtgg gtacatactg
    25741 aactccgtaa atcctggcat agagtttaat tcattcagga ataattcatt tttatttgtc
    25801 aaaaagaaat cgcaccggct caatccgctt ccacctaaca tcgtgtaagc taacttcgcg
    25861 tactcttgcg ctttttgata aacttcttct ggcacttcgg ctggaatctg catttcgatt
    25921 ttattattga tatattttgc ttcataatca tagaatgcta cgtcttttac gacttcacca
    25981 ggcaaagtcg tccgaacgtc ttcatttcct aatacagcaa cttcgatttc gcgcgcttca
    26041 attccttgtt caacgattgc tcgagaatca tactgatagg ctgttgctaa agcattttgc
    26101 agctcttctc ggttttctgc ttttgtaatg ccgacactag aacccatatt cgccggtttg
    26161 acaaacatcg gataaagcaa agaaccttca cattgatcaa atactttttt aggattttct
    26221 ttccattgat tcttaagtac tggtacataa ggaacttgcg gcacaccagc agcttgtaaa
    26281 atatacttgg tcatgatttt atccatggca catgcactgg tcaatacgcc tgcgccgaca
    26341 taaggcatat tcaatgtctc taagaagcct tggatcgttc catcttcccc gtttggccca
    26401 tgtaaaactg gaaaaacgat ggctccttct tctttgattt cgcccggatt gatcactttt
    26461 cctgtaaagc cttcctctgt ctgtccactt gggtcccatg aaagatgcaa gacatctttg
    26521 ctggcaggtt tttctgttag taatggacct ttgacccatt gtccttcttt tgtaataaat
    26581 acgagttgaa cttggtaata attataataa atggcattta aaactgaaaa tgcggaaaga
    26641 atggacactt catgctctgc gctgcgtccg ccatatagta aagtaatctt caaaagattg
    26701 tcctcctgta ttctaaatcg ttcagtgatt tactctcata ttttagcaca aaacaaaaat
    26761 aaacgaataa cttatttata aaaaaacgag ccagcttcta aaagaacgag cgtcatttat
    26821 tttttgtcag ctagattatt tgtcccgttt tttataaaaa gttatgataa agaaaaggag
    26881 ggatgatgat ggcgcaaaca aatccacgca gccgcttggc agaaatcatt cgagtgttca
    26941 tcaattacaa tgtagtgcca aactttgtac agcaaaaaaa tcccgaacag gtaaaaaaag
    27001 cctttgaaga actaggcccc accttcatca agataggaca gatgctttct gttcgagaag
    27061 atttgctttc ttctgccttc actcaaacat tcaaaacatt acaagatagt gtacctagcg
    27121 acacattttc aacggtcaaa aagacgatcg aaacagaact ttctctttca ttatcagata
    27181 tattcgatga tttttcaaaa tcgccttttg cctctgcttc tatggggcag gctcatcgtg
    27241 ccaagctgaa gaacggtgac gcggtagtcg ttaagatcca acaccctaac atcgccgaag
    27301 agatccgact tgatcttcaa ctatttgaac gagcgatccc tttgatcaaa tacattcctg
    27361 aaactagcgt agttgatcta aaaggggtac tccaagaagt aaaacgttcc ttgatcaatg
    27421 aaatggactt cttaaaagaa agtcaaaatg gcgaacaatt ctatcaaaag aacaatggct
    27481 ggaaagagat ccgcagcccc aagatttatg acgctttttg ttcgaaaaaa gtgatcgtga
    27541 tggaagaaat gtctggtaaa aacttgaatc atttaatgaa cgccgaaaac aaaactgaaa
    27601 cttttatcac tggaattcaa aacaagcagc tgaaacaaga agttgcaaag cttttagttg
    27661 agaactttat gaaacaggtg tttgatgatg gttttttcca tgctgatcct catccaggga
    27721 atctgctatt ccatgtacta acaaaagaag aacagacaca agctagccga aaaactgaaa
    27781 cagtccatga aaaagagttc ggttcctttg cttttcgagc ctccacttct gcagaagacc
    27841 ctgttgcgcc atataccatc aactacatcg attttggtat gatggggcat ctttctgcag
    27901 gacttaggca aaaactcaca caagccgttt tagcgctgta tacgaaagat gcttatcgaa
    27961 tcgaaaaagc ggtccttcga ctttgtcagc aagaaggctc gtttgatgaa agtcggttcc
    28021 accaagaact gacaagtttt cttgaacagt attatgattc tcctattgat gaaatcaact
    28081 tgcaagaagt attcacacat gtcgtgacga tttgtcatca aaataatctg cagttcgatc
    28141 gtgatatcac gcttttgctg aaagcatttg ggacattaga aggggtgatc cgcgtcctcg
    28201 atcctgaagt ttccctcatg gaagtcgctt cgccttttgc tcaacattat tttctcactc
    28261 atctggatgt agaagataca ttgaaacaaa gcggattaga cttattggaa gggatgaagg
    28321 ctgcgcctaa gatccctcaa cagctccacc atctattaga aatgtggact tcgggacaag
    28381 gaaaagtcaa tttggaattg aaaaagcaag acaaattatt gagcaggatc gaaagtatga
    28441 tcaatcggct tgttttcggt atgattttag ctgcattgat cgtagggtcc tccctgcttg
    28501 tccaagcagc acctgtagaa aacgcagagg tcgtttcctt attagggata tttacgtatg
    28561 ctattgccgc attcgtcatt atttttttgg caatcgatgc attgatccaa atgtataaga
    28621 agaggaaaaa gtagaaaaag gttgtgacag aaagaatcag cttcaagaaa taagaagggg
    28681 ttttacataa attgtttctc aaatttttgt gaaaattcgg cttatttttc gaagaggcta
    28741 cttctgaaac accgagaata aaaaatgtga aaaagctgtt ctgcacccag taataagaac
    28801 aatcaaaaag ggaacatgga acacatttat cctgtttccc tgttcccttt ttgatgtatt
    28861 cgagcataaa taaaatacct tcgttaccta ctatcttctg tacttggtaa accttagtca
    28921 attgcctttt ttaacgcttg acgaaaagac atatgatctt tctccgtaaa gggttttgga
    28981 ccctttgtcc ttatgccagc ttttcttaac tttccactat gataacgttg ttctagaagc
    29041 gtgccgatat tcgcttcgtc aaattctttt ccagagtgat ggaacactcg cacacccttc
    29101 ggcaatacaa gtgaagctaa accataatct tgcgtgatca aaaagtcatt cttcttaacc
    29161 ataccgacga ttttataatc cgcactatca gctccgcggt ctacgtagac gatttggaca
    29221 tgattagcgt attctttggt cgtataatgg tccacactgg tcacgataac cacgtcaaga
    29281 ccataggatt ctcctaattt tatgacatct tcttttactg gagaaccatc tccatcaatg
    29341 aatagcctca ttctttttcg ccttcttcca taaatgataa acagttacta atccgctcag
    29401 taaaagacca acattgatcc attttgccaa gataagcagt tgtacacttt ctatcggcac
    29461 tgattcatgg ttcagcgtga tcaataatag atggattggc tctcccattt cttttgctaa
    29521 aacaaacaaa agcgtcatca ataaaaactg gtggatcaca ctccctttat tttgagtagt
    29581 atacttgaca acgatccggc gatccattgg aatagctgta tcagtttgat aatattcctt
    29641 atttagccag ctgcctgtta ctacgtgcca gaaactaagc agaaagacac ctatcgcaaa
    29701 accatttgga atcaagagga ccaataaaga agaactcagt ccaagcaatt gcatagccaa
    29761 tggagaccat gttaaacgtt tcaaaatcca tggtccgact atttttgctg caatcaagcc
    29821 tgctgcataa ggcaaataca taaaaatcga aagtttttct aatccgtaca gtatgccaac
    29881 ataaaaactg ctaaataaaa aaaggaaatt ccctagcatt ccatttagaa aagtaaacag
    29941 atttagccag cttggcaact tccattcctt cttctggtgt cctaaaacaa acacgaacaa
    30001 gatgaacagc agcaagaaac cataaatagc aagatcaaaa agcctttcat cgaataacaa
    30061 tcgggcattt ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa caagctctcg
    30121 cgtagctacc agatttcttt tgatgtcttt gaaatccagt tcgtaacgcg gataatgact
    30181 aaccgtatgg taggccatga cgtaaaaaag tgtataaata gtaaagcttg ctgcaatctg
    30241 agtcggtgca ggcaactcaa tcgcttgata caggagaaat agccagatag ctgcaaataa
    30301 gtactttttc ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat tgaccgtggt
    30361 attagcaggt acaagccatg ctgcactcag ccctagcaaa acacttgaaa aataaaataa
    30421 aggaaaataa aatacgccta atattccaag caatgaacca gcaccaccaa tcagcagagc
    30481 gatcatcagc aacgtgtaac tatctagacc aaatcgtaga ctgttgacta aaaatagtcc
    30541 agtcatccga aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat tatgcgagtc
    30601 tgttagtccc aaaaataaaa gataaggaat aaaaataacc gtgttactta agataaaggg
    30661 cgttgtttgt gtaaaattac gcccaaatcg tttgagtttt tcgaacatga caatggctcc
    30721 ttaaacagtc ttataactaa tctgctcttc gattttagca ttaaagggga aataaaccaa
    30781 ttttacagaa tatattcttg taaaaaaaaa tacagaaaga agcataagct tctttctgca
    30841 tttttacggt acttgattac tcaagcagac aagcccttct ccacaaaagt cttgcccgac
    30901 acccctgcat ccggcaggtg ataaagatag aaatcaatta tcctgtgcct ccaaaatcaa
    30961 ttaggagctc tgtaaaccag agtccttctc gacccgtcgc ataaatttac tttaacacag
    31021 ttcctatgtt tatgcgagag cttttttcat tttataatcg accatttgaa atccttgacc
    31081 gatatctgaa ataagttctt ctgtaatctc aaatccttca tgcttataaa cctcgattgc
    31141 ttgatgattc ccctgattca ctcgcaaaaa gatctctctt tcctttgttt gcgcagcaag
    31201 ttcttcatac cagcgaaaaa ctgaagaagt caatccttga ccacgtactt tatttagcaa
    31261 atataattta cttaaataaa tttttccatt tatctcttca taagcagtgt aaccgacagt
    31321 cttttcttcg ctttttaata aaaagtagga cacaccttct gtcagttctt tttgaatctg
    31381 tttttttgac tgataagttt tcagcatgta atccacttgt ttttgaccaa tgatcggcac
    31441 aaaaacttcc ggccatactt cttgtatgac tgccatcagc tcatccagtt cattttctgt
    31501 tacttttatt ttttccatac acccacgtcc ttttttacat tataaatgac cttgcgcttg
    31561 atggaataaa aagagcatga tcatgctata cacacccacc gaccacggtt tcaagaaaat
    31621 cacaatctgc ataaattccc taaaagataa tttcgtcaaa ccagctacta gacagatcaa
    31681 gtcgtctggc gcaaagggaa gcagcaaggt aacaactagt acttttttaa tatctttcgt
    31741 ttttcttgtc agcaattcat caaatttctg atactttttt ggcgataaaa taagctggac
    31801 aaaggctacg ccatagtaac ggacaagcaa aaatccgatt gcttccccaa tcactaggcc
    31861 aatacaacta tataaaagcc caaatccatt gccaaacatc agcatgcccg ctactgagga
    31921 gatccctccc ggcaaaatag gaatgatcac ttggatcact tgcaacagaa caaatatcat
    31981 cactgcatac ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg atcgaaaaat
    32041 cccatgatga taagcataaa gaatcaacac aaagaaaaaa aacagaccaa taatgggcat
    32101 gatctgaaga catttttgta aaactgatcc ttttttagac atctatattc cccacatctt
    32161 ttttcattta ttaaaactgc ctcct
//
LOCUS       DACCXW010000019        32134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCXW010000019 DACCXW010000000
VERSION     DACCXW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.019"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            167..538
                     /locus_tag="IVY71_000702"
     CDS             167..538
                     /locus_tag="IVY71_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7984772.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(812..1504)
                     /locus_tag="IVY71_000703"
     CDS             complement(812..1504)
                     /locus_tag="IVY71_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7984773.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1507..2292)
                     /locus_tag="IVY71_000704"
     CDS             complement(1507..2292)
                     /locus_tag="IVY71_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7984774.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2529..3929
                     /gene="lacG"
                     /locus_tag="IVY71_000705"
     CDS             2529..3929
                     /gene="lacG"
                     /locus_tag="IVY71_000705"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7984775.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3954..4271
                     /locus_tag="IVY71_000706"
     CDS             3954..4271
                     /locus_tag="IVY71_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7984776.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4284..5957
                     /locus_tag="IVY71_000707"
     CDS             4284..5957
                     /locus_tag="IVY71_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7984777.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5971..7602
                     /locus_tag="IVY71_000708"
     CDS             5971..7602
                     /locus_tag="IVY71_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7984778.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8093..9658)
                     /gene="guaA"
                     /locus_tag="IVY71_000709"
     CDS             complement(8093..9658)
                     /gene="guaA"
                     /locus_tag="IVY71_000709"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7984779.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9717..10640)
                     /locus_tag="IVY71_000710"
     CDS             complement(9717..10640)
                     /locus_tag="IVY71_000710"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7984780.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10836..11441
                     /locus_tag="IVY71_000711"
     CDS             10836..11441
                     /locus_tag="IVY71_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7984781.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11749..12765
                     /locus_tag="IVY71_000712"
     CDS             11749..12765
                     /locus_tag="IVY71_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7984782.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12906..14207
                     /locus_tag="IVY71_000713"
     CDS             12906..14207
                     /locus_tag="IVY71_000713"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7984783.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14253..14915
                     /gene="deoC"
                     /locus_tag="IVY71_000714"
     CDS             14253..14915
                     /gene="deoC"
                     /locus_tag="IVY71_000714"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7984784.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14936..15334
                     /locus_tag="IVY71_000715"
     CDS             14936..15334
                     /locus_tag="IVY71_000715"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7984785.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15400..16482
                     /locus_tag="IVY71_000716"
     CDS             15400..16482
                     /locus_tag="IVY71_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7984786.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16694..18256
                     /locus_tag="IVY71_000717"
     CDS             16694..18256
                     /locus_tag="IVY71_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984787.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18249..19382
                     /locus_tag="IVY71_000718"
     CDS             18249..19382
                     /locus_tag="IVY71_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984788.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19382..20338
                     /locus_tag="IVY71_000719"
     CDS             19382..20338
                     /locus_tag="IVY71_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984789.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20437..21603
                     /gene="deoB"
                     /locus_tag="IVY71_000720"
     CDS             20437..21603
                     /gene="deoB"
                     /locus_tag="IVY71_000720"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7984790.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21627..22445
                     /locus_tag="IVY71_000721"
     CDS             21627..22445
                     /locus_tag="IVY71_000721"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7984791.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22462..23169
                     /gene="deoD"
                     /locus_tag="IVY71_000722"
     CDS             22462..23169
                     /gene="deoD"
                     /locus_tag="IVY71_000722"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7984792.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23467..24153)
                     /gene="gpmA"
                     /locus_tag="IVY71_000723"
     CDS             complement(23467..24153)
                     /gene="gpmA"
                     /locus_tag="IVY71_000723"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7984793.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24325..24906)
                     /locus_tag="IVY71_000724"
     CDS             complement(24325..24906)
                     /locus_tag="IVY71_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7984794.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25039..25722)
                     /gene="rpiA"
                     /locus_tag="IVY71_000725"
     CDS             complement(25039..25722)
                     /gene="rpiA"
                     /locus_tag="IVY71_000725"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7984795.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26041..26454
                     /gene="rpsL"
                     /locus_tag="IVY71_000726"
     CDS             26041..26454
                     /gene="rpsL"
                     /locus_tag="IVY71_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7984796.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26538..27008
                     /gene="rpsG"
                     /locus_tag="IVY71_000727"
     CDS             26538..27008
                     /gene="rpsG"
                     /locus_tag="IVY71_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7984797.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27102..29189
                     /gene="fusA"
                     /locus_tag="IVY71_000728"
     CDS             27102..29189
                     /gene="fusA"
                     /locus_tag="IVY71_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7984798.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29361..30548
                     /gene="tuf"
                     /locus_tag="IVY71_000729"
     CDS             29361..30548
                     /gene="tuf"
                     /locus_tag="IVY71_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7984799.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            complement(30727..32028)
                     /locus_tag="IVY71_000730"
     CDS             complement(30727..32028)
                     /locus_tag="IVY71_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7984800.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         9824 a         5912 c         7015 g         9383 t
ORIGIN      
        1 tataggaaat taaaaatatt atagaatcaa cactcagaga acgggttggt ttttaataga
       61 tttaataagt actgataatg aaatttctcc tggttgggaa aacgaagttg gttaccctgt
      121 agaagaattt tttcaagatt actttgcgga gtatgccgat aggataatga gagattacgt
      181 aacagataaa actcaagaag tcatcaatca atcccagtca ttaaatgaat tatctgataa
      241 ggcgaaagaa gaagcagttg ccattatttc ttcagggatt agtgactata ttgatatgag
      301 tggtgaaatc aattatggcg ataaagacaa aattgtacgc atgataaaag cttttatcaa
      361 aacttttgat gtgaaggcag atcttaagtt taatgatact tacctagtag gaaaattaat
      421 taatatcctt gggacagaga aagatacgga aacgtttatt tcctctttgt ctatgttgtt
      481 tacagataag catttttcaa ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc
      541 attacgccca gcattaataa aagtatatca aaaaagtact cctgatgatt tttaagattg
      601 gtttgagaaa taatgtgaaa acactatttt taatgttaat tatagataga gatgaacgcg
      661 tcatatggtt aaaagaatga aaaagtcaaa gatacactgc tatatttcac taaggatata
      721 gcagtgtatc tttcatgtcg tttcactggc agttttttat aaaaggaggt gtgcatttca
      781 gttgcttttc tttggtagtt agttgaatcg tttaagaatt aacggatgtg gcagacaggt
      841 gctctgtcca aaaaccagta aaatctttga tggttccatc tttatttagc tgtgcctgtt
      901 caaatactgt tttgttttta ccgaatggat caattgcaat aattgttccg atccccgcac
      961 gtttggcagc aagtaatcct gagtaagcat cttcgataac aagacaatca gccggagcta
     1021 agctcagttt gtctgccgct ttaagaaaaa tatccggttg cggtttccca gggaaagagc
     1081 cgtcgtcata gacaatcttg tcccagtcaa accagcgggc taaatcgaaa atatcaaaat
     1141 aaaaagcaac attctctttg acagtagccg tggcaatcgt taatggaatc tgttggtcag
     1201 tcagctggtc aagagtcgct tcaagccctt ttgtcaactg aagctcgttt tcattttgca
     1261 agcaaagcat acgataatag ctttcttttt cttctgacaa tacggcgatc tcagaggcgg
     1321 tcaaatgctc tgaaatgaaa tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt
     1381 ttaaaatatc ctcgtctgat aaaccatctt ttgtatgatc atgtatcata tgcagccaag
     1441 cagcttcatg cagatgagag tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt
     1501 tcatagttac gctcctatag tggtattttc ggttgaatca gcaatttgca gatcgacata
     1561 ttgtgcatag tgctcttgaa cctcccgcga gatctggcga tccgtaatga gcagatcgaa
     1621 atcatgcaaa ttataaaaca cataaaaatc atctcgattg aattttttat tatcaacgag
     1681 tagatatttc ttacgggaat tatttagagc gatctgctgt gcttctcctt cttcggtact
     1741 gtaagtagag atcgcttcgt tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt
     1801 tagtcttctt aaattgtcgt tggtgatcgg tccgacaaat gcgccggtat ttttccggaa
     1861 atcaccgcca atcagtagaa tttctggtcc atctgcttga gcaagaatat caaaaactgg
     1921 ataattattt gtaatgatcc gaattttctt gttcagtaaa tgcgtcgcca atagttcgat
     1981 tgttgtacct ggtcctaaga aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc
     2041 agcaatttct tttttttctt gaatctgcag agtggctttt tcagtgtgag aaagttcttg
     2101 atctaaagtg taggaaagac tttgagcccc tccatggaca cgcagcagct tgcccgattt
     2161 ttctaattca tctaaatctc tgcgaacggt catatcagaa acatctaatt gattgatgat
     2221 ttcattaacg gtaaggattc cttttttatt gaccatttct acgatagtaa gcaatctttc
     2281 ttttttcaac attgtcttca cctctaattt aaggataatc tatcatataa acggacaaca
     2341 gtcaatctaa ttcgaacaaa agtaaacatt tagggtggtt tttatgtttg ttacaataga
     2401 ttttattgct gttttctggt ttttatgatt atttgtgttt gtttttgttg acaaatgttt
     2461 tggatgcgtt tacaatgcaa tcagttagaa agagcgaaaa ctgattatag atttgaggag
     2521 agatgtacat gcttaaatta ccagaagatt ttatttttgg cggagccaca gcagcctatc
     2581 aggttgaagg agccactaag gaaggtggca aaggcgctgt tgcttgggat gactttttag
     2641 aggaacaagg gcgattcagt cccgatccgg caagtgattt ttatcatcaa tatgcaaagg
     2701 acattgaatt gtgtgaacgt tttggtgtaa atggtctaag attatccatt gcttggagcc
     2761 gcatttttcc tgatggagct gggaaaccaa atccagaagg aatcgctttt tatcatcgtg
     2821 tgttcgaaga atgtaagaaa agaaacgtaa cgccgtttgt gacgttgcat cattttgata
     2881 cccctaaacg tttgtttgac catggagatt ttttgaatcg agaaacaata gaagcatttg
     2941 tttcctatgc cattttttgt tttcatgaat tcaaagaagt caaggtgtgg agtaccttta
     3001 atgaaatcta tccagttgca accaatcaat atttgttagg tgttttccca ccaggaatca
     3061 aatatgattt tacaaagatc gtcgcctgtc ttcataatat gatggttgct catgcccgtg
     3121 tagtgaacta tttcaaagaa aacgaacttc cgggtgaaat tggtgtagtt cactcattag
     3181 agacgaaata tgcggcaaca gacgcgccgg aagataagca tgctgccttt ttagacgatg
     3241 cattgtcgat ccgtttctta ttagatgcta cctatttagg ctattattca actgaaacct
     3301 tgactgctct tgatgagata tgtgaagcaa atcaggccag ctatcatttt cctgaagaag
     3361 atttcgttga attgaaaaaa gcatccacgc gtaatgacta cttagggatc aatcactacc
     3421 aatgccattt tgtcaaagct tacgatggag aaaatgcaat ccatcataac ggtacgggtg
     3481 aaaaagggac ctctgtttat aaagtgaagg gaatcggaga acgtatttat aaagaaggaa
     3541 ttcctcgaac tgattgggac tggctgattt atccagaagg tctatatgac ttgttgctgc
     3601 gaattaaaag cgattaccca cattacaaca aaatctatat cactgagaac gggatggggt
     3661 ataaagatca gtttgaagat ggcattatta tggatcagcc gagaatcgac tatttgagag
     3721 tctatttgga atccttaagc aaggcgataa cagccggggt aaatgtgaag gggtattttt
     3781 tgtggtcgct tatggatctt ttctcatgga caaatgggta taacaaaagg tatggcttgt
     3841 tttatgttga ttttgaaacc cagaagcgtt acccaaaaga atcagcttac tggtacaagt
     3901 tggtcagtga aaccaaaacg attatttaat aagtgataat ggaggaagaa aagatgaata
     3961 aagaggaatt acaaatgctt ggttttgaga tcgttgcata ttcaggcgac gctcgcagta
     4021 cattattgaa attattgaaa gaagtaagac aaggaaactt tgaacatgtt gattcggcgt
     4081 tgaaagaagc cgacgaaaat ttaacccttg cacataattc gcaaacaaaa attttagcgg
     4141 aagaagcatc tgggaaagag atggagatgg gattcatttt tatccatggg caggatcatt
     4201 taatgaccac tttattgctg agagatctga ttcaagactt tattgtactg tacagacaga
     4261 atcaaggata ggagaagaac gctatggatg gaatcattaa acaaatcgaa aaagggaaac
     4321 cattttttga aaaaatctca cgtaatagtt atttaggagc cgttcgtgac ggatttttaa
     4381 cagcgatgcc agctatttta ttttcaagca ttttcatttt gattgctgcg gttcctgaga
     4441 tttttggtta ccaatggcct gaatctgtct ccacatggct atggaaagtc tataattatt
     4501 cgatgggtgt tgtagggtta ctcgtttcag caaccactgc tcgctgcttg gctgattcga
     4561 tgaatcgtcg cttaccgaaa aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg
     4621 tggtaagttt cctgttgtta agcgtctctc cgattgaagg tggattttca acggaatata
     4681 tggggacaaa gggactactg gcttcctttg tcgctgcatt tttaacagtt aatttatata
     4741 agttttgtgt gctgaaagat attaccatta aaatgcctaa agaagttcct ggaacgattt
     4801 cgcaaacctt ccgagatatt tttcctttct ctttttcagt atttgcggcc gttttaattg
     4861 atttaggcat tcgacaaatc ttccaaatct catttgcaga agcaatcatt acggtgctgc
     4921 agccgctatt tacagcagct gacgggtact taggaatcgc tattatctgg ggagcgatgg
     4981 cgctgttttg gtttgtcggt gttcatggac cgtcgatcgt ggagccggca attgcggcaa
     5041 ttatctatgc caatgtggaa accaatctgc aattgttcaa ttccggtgaa caagcttcta
     5101 aagtactgac ggtgggttta gggaattttg taggaactat gggaggaact ggggcaacat
     5161 tggttgttcc ttttatcttc ctactctttg ctaagtctaa gcagttgaag gcagttggga
     5221 aagcttcctt tattccagta agtttcgctg tcaacgaacc attgctattt gcggcaccca
     5281 tgattttgaa cccttatttt tttattccat ttttgttaac acctgttgta aacgtttgcc
     5341 tgttcaagtt ttttgtcgat gtattgggca tgaacagctt tatgtacgtt ttgccttggg
     5401 caactccagc accgatcggt ttggttctag gaacgggcat gggtatctta gcttttgttc
     5461 tagcagcggt tctaatcgtc gttgacttcc tgatctatct accattttgc aaagcatacg
     5521 atgctgtctt agtagagaaa gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg
     5581 cttctgccga tgtacgtgag gaagtacctg tggcagcgag tcaagttatc agcgatgcaa
     5641 ataaagaaat caatgtgttg gtactttgtg ctggcgcagg gacaagtggg atgttagcta
     5701 atgctttaaa aaaaggtgca tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt
     5761 atggttctca ttacgaaatg ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca
     5821 attcttacta tgatgatatc aaagaagaca ccgatcgctt aggaatcaag ctggttgcga
     5881 caaaaggagc agaatatatc ggattaacga gagatcctga aaaagctgtt cagtttattc
     5941 tgaaccaatt tcaataaagg aggagccata atgaaaatgg gaatttggtt gcgtaaagct
     6001 gttttatttg cagctgtgtt aggggctttg ggggctgttt caaaaactgt tgaagcatct
     6061 gccagcgaat ttattcgcgg agcagatatt tcgatcctgt cagatatgga aaaatctggt
     6121 gcaaaatatt atgaagacga tatttcaaaa gatgccttag agattttaaa aaataatggt
     6181 gtcaactatg ttcgactgcg tttgtggcag gatccttata atagtaatgg agaaagttat
     6241 ggagcaggga caaatgatct ggaaacgacg attgaccttg caaaacgagc aaaaaatctg
     6301 ggacttaagg tgctgctgga ttttcactac agtgattttt gggttgatcc tgggaaacag
     6361 aatcttccta aagcatggca aggtctgacc tttgaagaaa tgaatacggc attgtacgac
     6421 tatacaaaga acgttctgtc ggaaatgaag cagctggacg tttatcctga tatggttcag
     6481 atcggcaatg aactgaatag cggaatgctc tggccttatg gcaagtcttg gggagaaggc
     6541 gggggagaat ttgatcgatt ggctgctttt ttgaagtctg gtatccaagc tgttcgagac
     6601 actcaaccta aaacaactcc agtaatgctc cacttagctg atggtggcga tacaggagca
     6661 tttacttggt ggtttgatga aatcacgagt cgaggtgtgt catttgattt aattggagtg
     6721 tcgtattatc cgtattggca cggttcaatg aatgaacttc aaaccaatat gaatgcgatc
     6781 agccaacggt atggtaaaga agtgatcgta gttgaaacag cttatgggca tacaacagcg
     6841 aatgccgata ctatgccaaa tgcttttggt gaagccgaag cagctgcggg gggatatgaa
     6901 ccaacaccga caggacaagc agagtatttg ctggatcttg ccgatcggat tcaggcagta
     6961 ccgaataatc gcggggcggg cttcttctat tgggaacctc tttggtacaa cggaaatgtg
     7021 agttgggcaa cacaagcagg tatgaattat ctcagtgttc aatccactat gggaaacgaa
     7081 tgggacaacc aagcgatgtt tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt
     7141 aaacaagcag gggcgcaaat aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca
     7201 acgaccagtc cttccaattg gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc
     7261 aaaacggaag cgggcacttt ttttggggac tacaaactaa ctttttggca ggatcaagca
     7321 tacgaggcat cagcttatca aaatgttagt ggcataagca aaggaactta cacattgagt
     7381 gcctatgtaa tgtctggggg agagtttgat actaatcagt tatatatcaa aaattatggt
     7441 ggagcggaac taaatactgc ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa
     7501 gacattccaa ttacaacagg tgtttgtgaa attggggttt atagcaaggc ttcaggaaat
     7561 gcttggacga acgttgattt tgtttcattg gtgaaacaat agactgcgga tcgtttcgat
     7621 tcaagatcgg aggttccctt cagccacatc gttagcgtag aaggcaggaa tcgaacctgc
     7681 acctcgcctt tgccaaaaat aaccagaaac gatttccagt catttttacc aaaacgagta
     7741 aagcaccaga cttcatctac actttgtatt atgctaaaga gagttcaacg atttcgtaag
     7801 aagcaatgaa aaaattgcaa aaagttgtgc agataacata aagtttgttt aagaaagggt
     7861 aaccttagaa ttccgtcgtg aatatgattt gagcacttag atggatagaa aaaagttctc
     7921 ggaaaacagt tcgtttttcg agaacttctt tttatgataa gaatgaaaca aattaactta
     7981 tattatagtc tcttttggta gggaaccatc ttatttttaa tgaaaatcaa gcattttagc
     8041 tattgaagaa tgttgattta tagggattcc ccctccatat tatgttggct ctttattccc
     8101 actccactgt tgctggtggt ttagaagtga tgtcatacac gatacggttg acgtgggcta
     8161 cttcgtttac gatacgtaca gagatttttt gcaagacttc ccatgggata cgggcaaagt
     8221 cagcggtcat tccgtcgatt gaagttacag cacggatacc gactgtgtag tcgtaagtac
     8281 gtccgtcacc cataacaccg acagagcgga tccctggaag aacagtgaaa tattgccaga
     8341 tatcacggtc aagtccagca gctgcgattt cttcacgcaa gatagcatca gattcgcgta
     8401 cgatttctag cttgtcttca gtgatttcgc caagaacacg aataccaaga ccaggaccag
     8461 ggaatggctg acgccaaacg attgaatcag gcatgcctaa ctgagtccct aattcacgga
     8521 cttcgtcttt gaataatgtg tttaatggtt cgatcaattc aaattgcata tcttctggca
     8581 gtccacctac attatggtga gatttgatcg tctgtgctgt ttctgtacca gattcgataa
     8641 cgtcagtata caacgttcct tgtgctagga aagagacgcc ttcttcacca gcaagttttg
     8701 ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat gattttacgt ttttgttctg
     8761 ggtcagatac acctttcaac ttgtctaaga agcgatctct tgcattgact ttgatgatgt
     8821 tcaagccgaa ttttccgcct agactttcca ttacttgttc cgcttcgcct ttgcgtagca
     8881 gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc
     8941 caacgacact agaatcaacg ccacctgaaa gaccaagcaa gacttttttg tcaccaactt
     9001 gttcacggat tttttctact tgcatatcga taaagttatc cattgtccag ttaccttcac
     9061 agtgacaaac atcaaaggca aagtggcgca gcaattcgat tccgtattcg gaatgacgta
     9121 cttctgcatg gaattggata ccataaaaat tacgtgttgt gtcttggata gaagcaatcg
     9181 gacaatcttt gcttgttccg actgtttcaa agccttcagg aactttagtt accaagtcac
     9241 catggctcat ccatactgtt tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc
     9301 ttgttacttc taacaaggct ttcccatatt cgcggttttt agcaggctct acggaaccgc
     9361 caagtttgta cgtgatcaat tgcataccat agcaaatccc taatacagga atacctaaat
     9421 tgaagatctc aggatcgatc ccaaatgcat tctcatcata tacactgttt ggtccgccag
     9481 aaagaatgat tccctttgga ttcatcgctt ttacttcttc tgcagtaatg cggtggctca
     9541 ataattcgga aaagactcca agatcacgaa tacgtcttgt gatcaattgg ttatactgac
     9601 taccgtagtc taatacgata atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat
     9661 ttattacccc tatcccttta aattttatct caaaatagtc aaactatttt aaaatgctaa
     9721 tattttcgca tgaaaatcga atcgatgata tgattttcag ttttatggag aattatatcg
     9781 gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac atttttccat
     9841 acatcttttg ccattttaaa ggcttcttca cgatctccga tagcaaaacg ataataataa
     9901 ttctctggat tttggaaagc cgtatccagt aacgcaccaa aacgatccaa ataccatttt
     9961 tcgatcagca cgggatcggc atcgacatag acagaaaaat caaaaaagtc gctgacgtag
    10021 atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa gatatcaggc
    10081 tgctggatca attcgtactt gtctggaatg atatcataca catcatgtga gtaaacaggt
    10141 acttttatct cttcctgacc agattttaca cggttcaaga aatcgatcag catctccata
    10201 tcataacttt caggaaatcc tttacggtcc atgattccac gctcttttaa tatacgattt
    10261 ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac
    10321 aaaatcgtct gcagcagtct ggctgttgta ctttttccaa cggctacact tccggcgatc
    10381 ccgataataa aaggcggtac ttctacgtat ttatgaagaa ataaaccttt actcaagaac
    10441 aaagattcaa attctttcat ataaagataa atcagatgag caagaggtac atagatatct
    10501 cgaacatcct gcatggaaat gcggtcattc aaacttttga tattatctag ttcatcttgt
    10561 gtcaaaggtg cttgcccatt acgataaaat tctcgccatt cttctcttgc cacacggtag
    10621 tagttcattg gatcattcat attagcctcc tgcgttctcc catcaatgga actattttaa
    10681 cacaaaataa acgcaaaccc caccacttaa gagagattgc cgatcttttt gtataatctt
    10741 tataatagaa agaaacggag tcttattctt gacttgcgca cggatacata aaaggataga
    10801 ctgttgtcat gaaagaaatg agaaggatgg aagacatgac aaatcattac tattctgaac
    10861 gcccagatac ggcgcacgat tttgaacaat gggcttttga gttaaaaggc agaacctttc
    10921 aatttgtaac agattctggt gtattttcac gtgaaacagt ggattatggt tcacgtgtcc
    10981 tgattgatgc tttcgattgg gaagagttac ctgatgaagg aaagatctta gatgtgggat
    11041 gcgggtatgg accgattggg ttagctttgg catttgcgac acagcggttt gttgaaatgg
    11101 tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg
    11161 aacatgttga tatccatcaa tcgaatatct atgaagcagt tcatgaagaa acctatgcag
    11221 ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa attttgacag
    11281 gcgcatatcc tcggttgaag aagggcggta cactgacaat tgtgatccag aagaaacaag
    11341 gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa atcgtcacaa
    11401 aagataaagg atattatatc atcaaaagtg tgaaagaata actagctgaa aaatagtgga
    11461 gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg tatcagttct ttttttgttg
    11521 agaagttttt aaaaacaaag aactattttt tcttcaaaaa gaaatagtag gaacagagtc
    11581 gttgtttttt acgataaaat aaataagata cagaacttgt atagaaggct gccaagtcgg
    11641 tagataaagg aggtttttat caaaaaacat actttccttt tttctctatt cttttcttgg
    11701 gggttttggt acactgatag ggaatttaga tagaagggag ccaataggat gaagctgacc
    11761 atcagagata tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat
    11821 aacaaaggaa gcagatttag tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa
    11881 catcattata agcccgatta tttcgcttca aatgtgatca atcgacattc aaagaccatc
    11941 ggaatgatcg tccctgatgt cactgatttc ttcttctcca aactgatcga aggtgtagag
    12001 agctatttga atcctttggg ctatgtgatc atgttatgca attctcgtca tagccaagaa
    12061 aatgagatca agtatttaca agagctttcc caccgctcag tggacggaat cttgctggcc
    12121 acaccaaata ttttacctga gaaatatgca ttagacagcg gtttttatca aaaaatgcca
    12181 atcattttaa tcgatcgcgg attgaatcgt cgggataacg gacggcttat cgtaaaggaa
    12241 tatgagggcg cctatcaagc tgtttcgctg tttattgaaa atggccatac gaagatcggc
    12301 atgctgaagg aaaccactgg atactatcaa ttagaagaac gtttcaacgg atatcgtcat
    12361 gcgctgaaag atcatggcct gccttttaat gggaaatttg ttgaacatgg agagttgact
    12421 gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac ataaagatat cacggctatt
    12481 ttttgcggga atgatgccat ggcgatcgga tgctatcagg cgatcgatga attgggcaaa
    12541 aaaatcccag aagatatctc agtcatagga ttcgatggat taaagctgtc agaatacatg
    12601 attccgcaat tgacgacagt gcagcagcca agttttgata ttggatttta tgctgcacgt
    12661 ttcctgatcg atacgatcga atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca
    12721 aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg cggtatattg
    12781 cttatgtaag catttacaaa ggctagttag tgcttttttt atttttagta aaccagttta
    12841 ctaaagtggt ttacctttcc taaaaataat gtataatacc gaatgaagac aaggaggatc
    12901 aggcaatgcg aatggtggat ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag
    12961 aggaaatcga gtacatcgtc acgaactata cgaatggaaa gattccagat tatcaagtca
    13021 gtgcattatt gatggcaatt ttttatcaag acatgacaaa tgaagaaatc acgaacttaa
    13081 cattggcgat tgctaattct ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg
    13141 tagataagca ttcgacaggt ggcgttggtg acacgaccac attgatttta gcccccctag
    13201 tagctagtgt cggagtgaca gtggctaaaa tgtctggacg cggtctaggt tacacaggtg
    13261 gtacactaga caagctagaa gcaattcccg gatttcagat cgagctttcg gatgaagaat
    13321 ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg tcaatcagga aacttggcac
    13381 cggcggacaa aaaattgtat gccttgcgtg acgtcacagc gaccgttgat tctcttccgc
    13441 tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg
    13501 atgtgaccac tggagacggt gctttcatga agaacatcga agatgcaaga cgtctggcaa
    13561 agaccatgac gagcattggg aaactggcta accgagaaac agtggcggtt atctcagata
    13621 tgtcagaacc tcttggcgaa gctatcggaa atagtctaga agttgtcgag gcaatcgaaa
    13681 cattacaagg aaatggtcca gaagatctag tagaaatgtg ctacgcctta ggaagccaaa
    13741 tggttgttct tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg cttcaagaag
    13801 ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat caagggggag
    13861 acccagcgat cgtggaacat ccagaacgaa ttttgactgc acgctacaca atggagcttc
    13921 ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa
    13981 tgatgctagg tgctgggcgt aaaaccaaag aagaagatat tgatcacgct gttggattga
    14041 aactgcataa aaaaattggt gacactgtga caaaaggtga gtcactattg acgatctata
    14101 gtaacgacga agaaatcact tctgtcattg aattattata taaaaatatt gaaattggtg
    14161 aatcggcaat gaagccaacg ttgattcatg acatcatcac agaataaagg aaaaaagcgg
    14221 actttaccgt tttaaagaaa aggggaaaag aaatggaact aaaccgaatg atcgaccata
    14281 ctattttgaa agcagacgca tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa
    14341 aataccattt ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat
    14401 tacaaggtga gccagtagcc gtatgtacag taatcgggtt tcctctagga gcaaacacac
    14461 ccgagacaaa agcatttgaa acaacagatg ccatcaataa cggtgcagat gaagtcgata
    14521 tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca acaagtccaa aaagatatcg
    14581 aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt
    14641 tattagatag agaagaaatc atccgagctt gcgaaatagc aaaagctgca ggcgctgatt
    14701 ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa ggtagaggat gtccgtttga
    14761 tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc cataacgaag
    14821 aagaagcaat ggcaatgatc gaagctggtg ctacaagaat cggaacaagc gcaggagtag
    14881 caatcgtatc tggttcaact ggtgaaggct attaattttt tcaaaggaga agctcatggc
    14941 agaactcaaa caagaatgga tcaacacagc tattgaagcg ttagataaag cctatgttcc
    15001 ttattcccat tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg
    15061 catcaacatt gagaatgcat catttggatt aacgaattgt gccgagcgca cagctttttt
    15121 caaagcagta tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc
    15181 cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg gcagaattct gttcccctga
    15241 tatgccggtt acgctagtag gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact
    15301 gctgccttat gcatttactg aaaaagatct ataactcatg ttcattcagt cagctgactg
    15361 ttgaaaaata aaacaattat tttaggaggc tttacagaaa tgaaaaaagc aaaattattt
    15421 ggtttaggtg ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg caacaacggc
    15481 tctactgatt catccggtgg caaaagctca tcagacacaa caactgcagc attgatcaca
    15541 gacacaggtg gtgtagacga ccgttcattc aaccagtcag cttgggaagg actagaaaaa
    15601 tgggggaaag atcaaggact ttcacgagga aatgatggct tccaatattt ccaatcttca
    15661 aatgaatcag attacatccc aaatattgac caagcattga atgctggatt caaaacgatt
    15721 tttggtattg gttataaact aaaaccagcg atccaagaac aagcaacaaa caatacaggt
    15781 acgaactttg ttattatcga tgatgtcatc gatggattag ataacgttgt atctgcaact
    15841 ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag cagcttatac gactgaaaca
    15901 aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga
    15961 ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat
    16021 caatatgctg gtgacttctc tgctccagat aaaggacgtt caattgctca aggtatgtat
    16081 gcacaaaacg cagatatcat cttccatgct tcaggcggta caggtaacgg tgtattccaa
    16141 gaagcaaaat cattgaacga atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc
    16201 gaccaatcag acgaaggcga atacacatta aacggcgaaa agaaaaactt cacattgaca
    16261 tctacactga aagcagttgg aacagtagta gaagatttgg cacaaaaatc agcggacggt
    16321 aaattccctg gcggcgaaca cactgtttat ggattgaaag aagacggtgt tggtctaaca
    16381 gaaggacaac tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt
    16441 tcaggtgacg taaaagttcc agaaactcct gaagaaaact aaaaccattt ctatagcctt
    16501 gttagacaca tgtttgacaa ggctatggtt tgattagaac aagaattgtt catttatcca
    16561 agctgccttg tgacagtttg gataagggaa caactccctg aagtaatgtt tgtttcaaaa
    16621 gaagaacgct cactaagcgt gaatcttagt caatcgttgc cctagataga aatcgttagg
    16681 atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa atgcagaata ttaccaaaga
    16741 atttggaaca ttcaaggcaa atgacaacat caatttacaa gtaaaggcag gagaaattca
    16801 tgcgttgctt ggagaaaacg gtgctggcaa atctacattg atgaacgtgc tttccggatt
    16861 attagagccg acatcaggga aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc
    16921 gaccaaagcc aaccaattag ggattgggat ggtccatcag cactttatgc ttgttgatgc
    16981 ctttactgta acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga
    17041 ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa cagtatggat tgtcagtcaa
    17101 cccagatgct tacgttcgtg atatttcagt tggaatggaa caacgggtag aaattttaaa
    17161 aacactttac cgaggagcag atgtactgat ttttgatgag ccgacagctg tattgacccc
    17221 tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat
    17281 cattttgatt acgcataagt tagatgaaat caaagcagta gctgaccgct gtacagttat
    17341 ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac aacaattagc
    17401 cgatatgatg gttggaagaa cggtttcatt caaaacgatg aaaaaagaag cgaagcctca
    17461 agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt
    17521 gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg
    17581 aaacgggcag tcggagttga tccaagcttt gactggtttg cgaaaggcag aaagcggaca
    17641 tatcaagcta aaaggggaag acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg
    17701 tgtaggacac gtgccagaag accgtcataa atacgggtta gtcctagata tgacattatc
    17761 tgaaaatatc gcactacaaa cctatcatca aaaaccttac agtaaaaacg gcatgctgaa
    17821 ttattcagta atgaatgaac atgccagaga attgatcgaa gaatatgatg ttcgaacaac
    17881 gaatgaactt gttcccgcaa aagctttatc aggcggaaat cagcaaaaag caattatcgc
    17941 tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca aatccaactc gtggactgga
    18001 tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa caaagggaca aatacaaagc
    18061 agtgttactg atcagtttcg aattagaaga aattttaaat gtttcggatc gtattgctgt
    18121 tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt
    18181 aggtctattg atggcgggct ataccttaga agaagcacga aaagagttag aaaagggggc
    18241 tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt attagtgcct gtactttcag
    18301 ttgttttagg attgatctta ggcgcgatat tgatggctgc ttttggtttt cagcccatcc
    18361 aaggttattc ttctatgcta gatgcagccc ttggttcgca aagagggata ggtgaaacgt
    18421 taaggcaagc aacgccgttg atttttacag cattaggttt ttctgtagct aactcagctg
    18481 ggtttttcaa tattgggctt tctggacaag cactttgcgg gtggatcgtc agtgtctggg
    18541 tggcacttgc gtttccagat ctgccaaaag ttgttttgct tccgttatgc gtgatcttag
    18601 gtgccttagc tggtgcgatg gcagcggctg ttccaggttt acttcgagca ttttttggaa
    18661 caagtgaagt gatcgtaaca atcatgatga actatatctt attatacgta agcacatttg
    18721 ctttacatga agtgatgcct gaaagctatc gctcaagttt ggactcttcc aatatgatca
    18781 gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc ttttggcggc tctcgcgtaa
    18841 actcaggatt atttttagca atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa
    18901 cgactttagg atttgaaatc cgttctgttg gattgaatcc ttttgcttct gagtatgcag
    18961 gaatgagtag taaacgtacg atcgttttgt cgatggtcat ttctggcgct ttggcagggc
    19021 ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc
    19081 tatctattgg atttgacgga atggctgttt ccttgcttgg ttctggatca gcaatcggta
    19141 ttcttttatc agcgatcttg ttcagtattt tgaagatcgg tggacttggc atgcagacag
    19201 gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc tttgattatc ttctttgtcg
    19261 ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag
    19321 agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat
    19381 aatgtcgact atcgaattaa ttgccttgat ccttacttct acactggttt attcaacacc
    19441 gctaattcta acgtcactag gcggaacttt ttctgaacga agcgggatcg taaacgtagg
    19501 gctagaggga atcatggtca tgggtgcttt tagttccatc gttttcaact tgacgatgag
    19561 tccatcactt ggttctatga caccatggat cgctatttta gtgggtggcg tcgtaggtat
    19621 cctattttct ctgattcacg cagtagcaac agtcagtttg cgtgctgacc atattatcag
    19681 cgggacagta attaatctga tggcacctgc tttaggtgtt ttcttgatca aagcatggta
    19741 tggtaaaggt caaactgata acatcacgca gaatctgggg tatttttctt tcccaggatt
    19801 agcagatatt ccggtcattg gaccaatttt ctttaaaaat acattcttac ctgcttattg
    19861 tgctattttg attgctgtcc tagcttgggt gatcatcttc aagacgaaat tcggcttgcg
    19921 cctccgttca gttggtgaaa atcctcaagc tgccgacaca ttaggtatca atgtatatgc
    19981 tatgcgttat tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt
    20041 tgctcagtcg atttccggta acttttcagc tggtacgatc gttggacaag ggttcatctc
    20101 catggctgct atgatctttg gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt
    20161 ctttggtttt gctcaaagct tgagtatcat tggcgcacaa ttaccagtta tttcttcgat
    20221 tccgccagta ttgctgcaaa ttgctccata tttgctgaca atcattgtgc tggttgtttt
    20281 cttagggaaa gcttctggtc caaaagcaaa cgggaagaac tatatcaaat caaaataatc
    20341 ttttagcgaa taaaaagggt gggttacggg aagtgttttt cgtctcgccc tttttgtttt
    20401 aaaggcaata aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt acatctgatc
    20461 gtcatggatt cagtaggaat tggagaagca ccagatgccg ataagttcgg tgatgttggt
    20521 tctgatacat taggacacat tgcgaaagaa gcaggattga acgttccaca tttagagcag
    20581 cttggtttag gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc
    20641 tatgcgacga aattagaaga agtttctgta gggaaagata cgatgacagg ccactgggaa
    20701 atcatgggat tgaatatcaa aactccattt cgtgtgtttc ctaatggatt ccctgatgat
    20761 ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa accttatagc
    20821 ggaacagctg ttattgacga ttacggtgaa caccaaatga aaacaggcga tttaattgta
    20881 tatacttctg ccgatcctgt ccttcagatc gcggcacatg aagaaatcat tcctttagaa
    20941 gaattatatc ggatttgcca attcgttcgt gatataacaa aagatgatcc gtatatgatt
    21001 ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg aacaagcaat
    21061 cgccatgatt atgcgttgaa tccattcgga cacacagtcc ttgattcttt gaaagaagca
    21121 ggaaaggacg tgattgctgt tggaaagatc aacgatattt tcaacggaca aggaatcact
    21181 gaatctgtcc gcacgaaaag caatatggat ggcgttgacc agctgctgaa agttatgaaa
    21241 aacgattttt caggaatcag ctttaccaat ttagtagatt ttgatgcatt atacggtcat
    21301 cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat ttgacttacg aattccagaa
    21361 ttgcttgaag cgatggaaga agacgacttg ctaatgatta cagccgatca cggaaacgat
    21421 ccgacgttta ctggtaccga tcatacgcga gaatatgtac cgttattggt ttacagtaaa
    21481 aaaatgaaag aacaaggggc attgcctcaa ggatattatt cagatatttc agcaacgatt
    21541 gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa
    21601 taatcttttt aaataggaga aagcaaatga ccaagttaag cgagatgtta caagaaacga
    21661 cccagtttat ccttgataaa ggagtaaaag aaattgattt tggtttgatt ctaggttcag
    21721 gattaggtga attagcagat gaaatcgaag atgctgtaat gatttcttat gatgagatcc
    21781 cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa gctagtttat gggtctctgg
    21841 caggcaagaa agtgttagcc atgcaaggga gattccacta ctatgaaggt cattccatgc
    21901 aaacagtgac ctatccagta cgtgtgatgg ccggactagg ggcccattca gtcattgtga
    21961 ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg agatttgatg ctgattaccg
    22021 atcatatcaa ttttactggt gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc
    22081 ctagatttcc agatatgagt caagcttata cgcctgacta tcaagcaaca gcaaaagcag
    22141 cagcagaaaa actaggtttg gatcttaaag aaggcgtata tatgggctat tctggaccaa
    22201 cttatgaaac gcctgcggag atccgaatgt ctcggacaat aggagccgat gcagtaggga
    22261 tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg attaaaagtg ttaggaatct
    22321 cttgtattac caacttagca gcaggtatgc aagcgaatct gagccatgaa gaagttgtag
    22381 aaacaactca gagagtcaaa caatctttta aagcattggt taaagaaaca ttgacattat
    22441 tgtaatagga ggaacaataa aatgagtgtg catattgaag cgaaagaagg cgagatcgcc
    22501 gataagattt tacttccggg agatcctttg cgagctaaat atattgcgga gacttttttg
    22561 gaagatccag tttgttacaa tcaggtacgc ggtatgttag gctttaccgg caaatataaa
    22621 ggagaacgta tttctgttca aggaacagga atgggaatgc catcagccac catctatgcc
    22681 catgaattga tccagtcgta tggtgtaaag aaattgattc gagtaggtac atgcggtgca
    22741 ttgtcaaaag acgtccatgt tcgtgattta gtactagcac aaggtgcagc gaccagttct
    22801 tcgatgatcg aaaaaaattt ccaagccttt cattttcctc caatcagtga tttcaatcta
    22861 ttactgaaag catatgagat agctaaagaa aagggctata cggttcatgt aggaaatgtt
    22921 ctttcggaag attctttcta caaagatgat ttgacagaga cgttccaatt agctgaactt
    22981 ggcgttttag gagtggaaat ggaagcagcg gctctttatt acttaggcgc gaaataccat
    23041 gtgcagacat taagtttgat gaccgttagc gaccatttga tcaccggtga agaaacaaca
    23101 gctgcagaac gtcaatctac gttcaatgaa atgatcgaag taggattgga aacagcgata
    23161 gcagaataag aaggaaatga aaaagctgcc ctgcattaag taataagaac atccaagcaa
    23221 aaatagctcc tcatgttttt gacttggctg gacttattat tgcaaatgta agcttttgaa
    23281 caccgtttac caagaggttg tgaatctgcc gtttaactct gagtcacaag gaacaatttc
    23341 aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt gaccgagaag ttggcaggag
    23401 aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg tcacagtccc ttttttatag
    23461 tgttttttat aggtagtatt ttttagcaac tgttaagtca tcatttaatt cataaacaag
    23521 tggttgacct gttggaattt caagatccat gatgtcttcg tcagagatac cttcgatatg
    23581 tttagctagg gcgcgtaatg agttaccatg tgcagctact aaaacagttt tgttgtctag
    23641 taatgctgga gcaatttcat cttgccagaa tggcaaagcg cgttctaatg tcactttcaa
    23701 gttttctcca ccaggaatat cgcgtttgtc taacattgcg taacgacggt catttgctgc
    23761 agaaccttcg tcagtttctt ccattaatgg aggaagagta tcgtaagaac gacgccagat
    23821 gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt ttgttcaaac cttgtaattt
    23881 accatagtga cgttcgttta agcgccatga tttgatttgt ggtacccaaa gttgatctga
    23941 atattctaag atcaagttac atgttttgat tgcacgagtt aaaacagaag tgtaagcaac
    24001 atcaaattcg attccagctt ctttgatttt gcgtccgcct tcttttgctt cttcgattcc
    24061 ttctggtgct agatttacat cagcccaacc agtaaattgg ttcaatgcat tccattcact
    24121 aagaccgtga cgagaaaaaa ctaattttgg catgtttagc tctcctttta agataggttt
    24181 atatttctga aattttcagt tctgttttat tgtacaattt tttggaagag atttccatcg
    24241 aaaaattaga taagattcta tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc
    24301 cacgcaactg ttcgttttta ggtattagat gcataattgc tcgatttctt tttcaggtaa
    24361 attgatatat ttataacatg ttccaactga aacaccgcat tttgaagaaa taaattggat
    24421 cgtttctttt ttttcataat agagacgtcg gatttttttg atcgtttttg catcgatttt
    24481 tggtcttccg ccgatttttc cttttttacg agcttcatct aatccaacta atgtacgttc
    24541 tttgatcaat gcgcactcca tgtttgctag gttttccatt aattggaaat agatttttcc
    24601 catttcgcgc gtatcgattc cttcatcaag actaatcaag tgcagcccgc gatctttgaa
    24661 taattgcgtg agttctgtta attgccgcgt agatttgcct aatcgatgaa gttggcaaat
    24721 gactagtgca tcgccttgtt gcatgtcagc gatcactgac tccaaatgaa cagcagtaga
    24781 atcattttgt tccacttcat atgtctcatg gaaaatttct tgacaaccaa agtcagataa
    24841 ttgatcaacc tgagttgtta aatcaacgtc atttatggtt gtgcgtgcat aaccaataat
    24901 tttcatggtg attcctttct agcagtgatt ttctaactgg atataacagt aaaaatgtag
    24961 cataaagtac gtcaaaaaga tagttttttc gcaaattttc cgctgtcttt ttttattgga
    25021 tgatcaagca gtttttcttt atttacgacg gattgcgtca agaatctcaa cgcctttttc
    25081 tgagccgaca actactttag aaaccatgtt taggaaaagc ccatgttcta ctaccccaac
    25141 aagttcatct aaataggtag ctaagctttc tggctgttcg atgacttcta gatgcagatc
    25201 aatgatgtaa tgaccaccat ctgtggttaa gacttctccg ttttcgtctg tacgcaagac
    25261 gggttggaat cttttttctt caaaaatatg aagtagctgt tgggagccat aagggatcac
    25321 ttcaactggg agtgggaact tcccaagttt atcgactaat ttagaagaat caacgatcca
    25381 aatcgtttct tttgagtacg tagcgactat tttctcgaaa agcaaagcag cgccgccgcc
    25441 tttgatccct tgaaaatcag cactgatttc atcagcaccg tctattgtca aatcgactac
    25501 ggggacttca tcaatactct ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc
    25561 atttgaagta gtaactcctg tgatacgtaa accttcttca cgcatacggc gaccaatctc
    25621 ttctaccata taataggcgg ttgaaccggt cccgagacct acgatcatcc cgtcctttac
    25681 gaatttcgcg gcttcaatgc cagctaattg ttttaaattc atctttgcgt ccaccttttt
    25741 ttagtttgag catcgtgctt tcctcactat tttagcgtga tttacggtaa aaagatacca
    25801 ttctttttta ttccaataaa gctttttcaa aaaaaattgc atctcttgtt gacatgtttt
    25861 ttcattcgtg atattctaac aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct
    25921 gactgtttat caaagcatat ttgataagga tgcagacatt gcatttattt tttatcacaa
    25981 aaaaagaacc acctggatgt gtggaactag acgcaaatcg tgggaaggag gaaacaagga
    26041 atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat
    26101 tcacctgctt tgaacaaagg atataacagc tttaagaaaa ctcaaactaa cgtaaactca
    26161 cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga cacctaaaaa accgaactca
    26221 gctttacgta aatatgcacg tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc
    26281 ccagggattg gtcataactt acaagaacac agtgttgtat tattacgcgg tggtcgtgta
    26341 aaagacttac caggggtacg ttaccatatc gttcgcggtg ctttagatac tgctggtgtt
    26401 aacgaccgta aacaaagccg ttctaaatac ggaactaaaa aacctaaagc ttaattttaa
    26461 ttattaaaca aatgaaactt gcttctacta tatagaagta attactatga acaatatttc
    26521 ggaaggagga gttacggatg ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag
    26581 atcctattta taactcaaaa ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa
    26641 aacgcgggat tgcagctaac attatctata atgcctttga cttgatcaaa gaatctacag
    26701 gtaacgatcc attagaagtg tttgaacaag caatgaaaaa cgttcaacct gttcttgaag
    26761 ttaaagcacg tcgtgttggg ggttcaaact accaagtacc agttgaagtt cgccctgaac
    26821 gccgcgttac tctaggtctt cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca
    26881 caatggaaga acgcttagca aaagaaatca tggatgctgc aaacaacaca ggtgcttctg
    26941 ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc
    27001 gctggtaaga ttctctctgt ttggtcggct tttataagtc gacctcagat agtgtcctta
    27061 tttttagcaa ctagatagta aagagaggag aaacaaggag aatgaccaga gaattttctt
    27121 tacaaaacac tcgtaatatc ggtatcatgg cccacatcga tgctggtaaa acgactgcta
    27181 ctgaacgtat cttgtactat actggtaaaa tccataaaat tggtgaaact catgaaggag
    27241 cttcacaaat ggactggatg gaacaagaac aagaacgtgg tattacgatc acttctgctg
    27301 ctactacagc acaatggaag ggtcaccgaa tcaacatcat cgacacacca ggacacgtag
    27361 acttcacagt tgaagttgaa cgttcattgc gtgtacttga tggtgctgta actgttcttg
    27421 atgcacaatc aggtgttgaa cctcaaactg aaacagtttg gcgtcaagca acaacttatg
    27481 gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat cggtgctgac ttcttatatt
    27541 cagtatcaac tttacatgac cgtttgcaag caaacgctca cccaatccaa ttaccaattg
    27601 gtgcggaaga cgacttcaca ggaattatcg accttgtaac aatgaaggct gaaatgtata
    27661 caaacgatct tggaacagag atcgaagaaa cagaaattcc tgaagaatac cgcgaattag
    27721 ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga aactgatgaa gaacttactt
    27781 tgaaatactt agaaggtgaa gaaatcactg aagctgagtt aaaagaaggt atccgccgcg
    27841 caacagttaa cgtagaattc tacccagtac tatgtggttc agcatttaag aacaaagggg
    27901 ttcaattact attggacgct gttcttgatt acttgccatc accgttagat atcccagcta
    27961 tcaaagggat tgaccctaaa actgatgaag aagtagaacg tcctgcagac gacagcgctc
    28021 ctttctcagc tttagcattt aaagttatga ctgacccatt cgtaggtcgt ttaactttct
    28081 tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt acaaaacgca acaaaaggca
    28141 aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa ctctcgttca gaaatctctg
    28201 aagtttacgc tggagatatc gctgctgctg ttggtttgaa agacacaact actggggaca
    28261 cactatgtga tgaaaagagt ctagttattc ttgaatccat ggaattccca gaaccagtta
    28321 tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga taaaatgggt gtggctttac
    28381 aaaaactatc tgaagaagat cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa
    28441 caatcatcgc tggtatgggt gaacttcact tagatatcat cgttgaccgt atgcgtcgtg
    28501 aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg
    28561 ctggcactca agccgaaggt aagtttgtac gtcagtctgg tggtaaaggt caatacggtc
    28621 acgtatggat tgaatttaca ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga
    28681 tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt tgaaactggt ttgaaagatg
    28741 ctatggaaaa tggtgtctta gctggctatc cattagtaga tatcaaagct aaactttacg
    28801 atggttcata ccatgatgtc gattcaaatg aaactgcctt ccgtgttgct gcttctatgg
    28861 cattacgtgc agcagctaag aaagctaatc cagtaatcct tgaaccaatc atggctgtag
    28921 aagttgttat ccctgaagat tatttaggtg atgttatggg tcacgtaact gctcgtcgtg
    28981 gacgtgttga aggtatggaa gctcgcgctg gcggccaaca agttgttcgt gcgatggttc
    29041 cacttgctga aatgtttggt tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta
    29101 catttacaat gacatttgac cactacgaag atgtaccaaa atcagtacaa gaagaaatca
    29161 ttaagaaaaa cggcggaaaa gctgaataat ttcttgaaac cggatagtcc tgcaaagaga
    29221 ttatccaaag agactatatt tttaatatag tttcgtgtaa aataattatc gatgatatgg
    29281 gcatgtggtg taccttgtgt acatcatata cctatcagac aaataaaaaa tatattgtat
    29341 ttttaggagg acattttaaa atggcaaaag aaaaatttga ccgttctaaa ccacacgtta
    29401 acattggtac tatcggacac gttgaccatg gtaaaactac attaacagct gcaatcacaa
    29461 ctgtactatc taagaaaaac ggcggccaag ctatggctta cgatcaaatc gatggtgctc
    29521 cagaagaacg cgaacgcgga atcacaatct caacagctca cgttgagtat gaaacagata
    29581 cacgccacta cgctcacgtt gactgcccag gacacgcgga ttacgttaaa aacatgatca
    29641 ctggtgctgc tcaaatggac ggagctatct tggtagtttc tgctgctgac ggcccaatgc
    29701 ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg tgttccttac atcgttgtat
    29761 tcttgaacaa agtagacatg gttgatgacg aagaattact agaattagtt gaaatggaag
    29821 ttcgtgacct attaacagaa tacgaattcc ctggtgacga tgttcctgta gttgctggat
    29881 cagctttgaa agctctagaa ggcgacgctt catacgaaga aaaaattctt gaattaatgg
    29941 ctgcagttga cgaatacatc ccaactccag aacgtgacaa cgacaaacca ttcatgatgc
    30001 cagttgaaga cgtgttctca attactggac gtggtactgt tgctacaggt cgtgttgaac
    30061 gtggacaagt tcgcgttggt gacgaagttg aagttgttgg tattgctgaa gaaacttcaa
    30121 aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt agactacgct gaagctggag
    30181 acaacattgg tgctttacta cgtggtgttg cacgtgaaga catccaacgt ggacaagttt
    30241 tagctaaacc aggtacaatc acacctcata caaaattctc tgcagaagta tacgtgttga
    30301 caaaagaaga aggtggacgt catactccat tcttcactaa ctaccgtcca caattctact
    30361 tccgtacaac tgacgtaaca ggtgttgttg aattaccaga aggaactgaa atggttatgc
    30421 ctggcgacaa cgtaacaatg gaagttgaat taatccaccc aatcgctatc gaaaacggta
    30481 ctaaattctc aatccgtgaa ggcggacgta cagttggtgc cggtgtcgtt acagaaatca
    30541 aagcttaatt cacttaagtt ttaaagtaaa gcaacattta ttcggacgta atcgctcgga
    30601 acgaaggtct taggatcttt gttccgagct tttttattta ttgcaggatc aggaaaaaag
    30661 ggctctttgt caaataggac tgatagagtg agataagaac atctaaggta gaattccttt
    30721 tctactttag atgttttttg attcataggt aatcagatta tttagtaaga aaattctggt
    30781 cttcattttc cgaaagcttc gaaagccata agcgactcgt ttcaatgctt tgatgtgggt
    30841 atgcagattt tctattttgg catttgaata aggcaattcg agggctaaac gaatgccgga
    30901 ttcatagttt ataaaagctt tttttataga atgcagttct tttggaacgg aattgggcat
    30961 cgttcttaca agtttaaaga aagtgtccgc ctctttgtcg tgaaacgctt gaagaatttc
    31021 ttgatagtaa aaataggcga ttcgtaattc gtcagagatc gaaagcagac gatccaccac
    31081 ttcactttcg gtgagatacg cccaaccaaa attgcgccat ttggtccatt tgtcataaac
    31141 aagcttgtcc tgtcgtttga tcagaagccg agacaacgcc tttaattgtc gataatattt
    31201 ggcttgtgtt ggatcgtttc gatcaaagct tttcatgatt cgtacgcgaa tatcctcaaa
    31261 tttacgaatg agatgtttga caatgtgaaa acgatccaca acgagttttg cctttggaaa
    31321 acattccttt atcagagagg cataagaaac attcatgtcc gtcacgatga cctttacctt
    31381 caatcgtgcg cgtcgttcaa attgcataaa ataagaaacg agagcctttt tcttccgtga
    31441 aggaagaata tccagtaatt ttccagtatc gccatccata caagaaaagg agtagggacc
    31501 gacttgattt ttcagtgaac gaaattcatc cattaataaa caacgcggta accaatttct
    31561 ttttactttt gtcatgggga tcagagaagc gagtgtccga gaaacggtcg accaagaaac
    31621 gccgcaatgc ttagcaattg tcgtcattgc ttgtttttca gttagctctt ctaaaatttt
    31681 atagcgcact tgtttagcga taaagcagtt tctatcgaca atgtaggtat tcgccgtaaa
    31741 ataggtgtgg cagccacgac agtggtagcg ttgtttcgct aaagcaagat aagtcagttg
    31801 gttcgcacat ttattaagtt gaatgagcga ttttttcttt ccgttcttaa cgatcatgcc
    31861 ttcatgattc atctgtttac acaaaggaca gcgacgaatt tctttactta acgtcgcaga
    31921 aacaacaaga ctcttgatat tcttaatgct cttcgattcc acatttgtga tgaaaatgtt
    31981 tttatctttg attccaagta gtagtttgat atgatgaaga tcaggcatac agaacgctcc
    32041 tttgattgtg ttgtggtgat tcaattctaa cggagcttct gtatgttttg ttttttcatg
    32101 ctcaaaaggg accgatagaa tgattttcta tcag
//
LOCUS       DACCXW010000020        31195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCXW010000020 DACCXW010000000
VERSION     DACCXW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.020"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            214..1638
                     /locus_tag="IVY71_000731"
     CDS             214..1638
                     /locus_tag="IVY71_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7984801.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            2099..3208
                     /gene="pdhA"
                     /locus_tag="IVY71_000732"
     CDS             2099..3208
                     /gene="pdhA"
                     /locus_tag="IVY71_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7984802.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            3211..4188
                     /locus_tag="IVY71_000733"
     CDS             3211..4188
                     /locus_tag="IVY71_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7984803.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            4213..5856
                     /locus_tag="IVY71_000734"
     CDS             4213..5856
                     /locus_tag="IVY71_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP7984804.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            5864..7270
                     /gene="lpdA"
                     /locus_tag="IVY71_000735"
     CDS             5864..7270
                     /gene="lpdA"
                     /locus_tag="IVY71_000735"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7984805.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            7432..7707
                     /locus_tag="IVY71_000736"
     CDS             7432..7707
                     /locus_tag="IVY71_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7984806.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            7713..8480
                     /locus_tag="IVY71_000737"
     CDS             7713..8480
                     /locus_tag="IVY71_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7984807.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            8648..10477
                     /gene="typA"
                     /locus_tag="IVY71_000738"
     CDS             8648..10477
                     /gene="typA"
                     /locus_tag="IVY71_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7984808.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            10738..12510
                     /gene="recQ"
                     /locus_tag="IVY71_000739"
     CDS             10738..12510
                     /gene="recQ"
                     /locus_tag="IVY71_000739"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7984809.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            12624..12926
                     /locus_tag="IVY71_000740"
     CDS             12624..12926
                     /locus_tag="IVY71_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7984810.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            12929..14092
                     /locus_tag="IVY71_000741"
     CDS             12929..14092
                     /locus_tag="IVY71_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7984811.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            14118..17546
                     /locus_tag="IVY71_000742"
     CDS             14118..17546
                     /locus_tag="IVY71_000742"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7984812.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            17567..18691
                     /locus_tag="IVY71_000743"
     CDS             17567..18691
                     /locus_tag="IVY71_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984813.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            18681..19118
                     /locus_tag="IVY71_000744"
     CDS             18681..19118
                     /locus_tag="IVY71_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7984814.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            19102..19440
                     /locus_tag="IVY71_000745"
     CDS             19102..19440
                     /locus_tag="IVY71_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7984815.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            19559..20119
                     /gene="rsmD"
                     /locus_tag="IVY71_000746"
     CDS             19559..20119
                     /gene="rsmD"
                     /locus_tag="IVY71_000746"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7984816.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            20116..20607
                     /gene="coaD"
                     /locus_tag="IVY71_000747"
     CDS             20116..20607
                     /gene="coaD"
                     /locus_tag="IVY71_000747"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7984817.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            20597..21640
                     /locus_tag="IVY71_000748"
     CDS             20597..21640
                     /locus_tag="IVY71_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7984818.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            21799..22527
                     /locus_tag="IVY71_000749"
     CDS             21799..22527
                     /locus_tag="IVY71_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7984819.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            22643..22888
                     /locus_tag="IVY71_000750"
     CDS             22643..22888
                     /locus_tag="IVY71_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7984820.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            22885..23103
                     /locus_tag="IVY71_000751"
     CDS             22885..23103
                     /locus_tag="IVY71_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7984821.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            23239..23778
                     /locus_tag="IVY71_000752"
     CDS             23239..23778
                     /locus_tag="IVY71_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7984822.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            23771..26044
                     /locus_tag="IVY71_000753"
     CDS             23771..26044
                     /locus_tag="IVY71_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7984823.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            26082..27128
                     /gene="holA"
                     /locus_tag="IVY71_000754"
     CDS             26082..27128
                     /gene="holA"
                     /locus_tag="IVY71_000754"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7984824.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(27217..27471)
                     /locus_tag="IVY71_000755"
     CDS             complement(27217..27471)
                     /locus_tag="IVY71_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7984825.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(27871..28140)
                     /locus_tag="IVY71_000756"
     CDS             complement(27871..28140)
                     /locus_tag="IVY71_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984826.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(28610..29065)
                     /locus_tag="IVY71_000757"
     CDS             complement(28610..29065)
                     /locus_tag="IVY71_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7984827.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(29203..31131)
                     /locus_tag="IVY71_000758"
     CDS             complement(29203..31131)
                     /locus_tag="IVY71_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7984828.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        10419 a         5422 c         6609 g         8745 t
ORIGIN      
        1 aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg aattattatc
       61 gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa gatttttgtc
      121 acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc ggtaaaatca
      181 aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga gaaaaaacaa
      241 ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa cggaaggatc
      301 aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg tgtgaatcgt
      361 tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac gagcaaacgt
      421 ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc aagaatcgat
      481 tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca aaaaataaga
      541 gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga gatgccagat
      601 gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt agcggaagaa
      661 aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg gtatttggaa
      721 agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg cggtcagcaa
      781 gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc tgtagagtca
      841 ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt atttgggata
      901 ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca taaaaataaa
      961 ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt gatgagtcaa
     1021 aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat tattgaagac
     1081 gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat acgaacgatt
     1141 gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa aacaacgaaa
     1201 atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc gcaagaaatg
     1261 atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt taatgaagca
     1321 attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg actgataagc
     1381 gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg ttactatgcc
     1441 tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt agtagatatg
     1501 ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat cagaatcaat
     1561 tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa aattctagaa
     1621 aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac agcttggatt
     1681 taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga aactaagaaa
     1741 aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt gcaacaaaac
     1801 tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt agaaaataga
     1861 aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac taatgctgtt
     1921 aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta gcttgtattt
     1981 ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca gttttattca
     2041 ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg tgtgacaaat
     2101 ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca atgcagattt
     2161 cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt tagtacctga
     2221 tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc gtgtattaga
     2281 ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac caactgctgg
     2341 acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg ttcttcttcc
     2401 aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc aagctttttt
     2461 atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag ctctaccacc
     2521 acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag gtttgaagaa
     2581 acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat cacaaggtga
     2641 tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat tttatatcca
     2701 aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa aaacacttgc
     2761 acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg atccgcttgc
     2821 agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg gaccagtcct
     2881 aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg atccaacccg
     2941 ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga tccgcttccg
     3001 taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag tcatcgaagc
     3061 aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa aacaaaaggt
     3121 ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag aacaaattgc
     3181 aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat ccaagcaatc
     3241 acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt tggtgaagat
     3301 gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa gttcggagaa
     3361 gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag tttcggtctt
     3421 gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat tttcgaagca
     3481 atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg cactagaaat
     3541 ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga attgcattcg
     3601 gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat tccttccaac
     3661 ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc ggttgttttc
     3721 ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga agcttacgaa
     3781 gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat tatcacatac
     3841 ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga aaatatatct
     3901 gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat cattcaatcc
     3961 gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc gggcgttgcc
     4021 gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc cccaatcgga
     4081 cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt ctggttgcca
     4141 aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga aaagaaggga
     4201 cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg aatcgcagaa
     4261 ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga tgatacatta
     4321 ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac aggaacggtt
     4381 aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt agtagaaatc
     4441 gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga atcccaaact
     4501 ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc gtcaagcgaa
     4561 ggagtcttcc aatttaagtt accggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc
     4621 aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa
     4681 aatgacaaat cagtagaaga aattccgtca cctgtcacag ggacagttaa aaacgtgatt
     4741 gttccagaag gcactgtagc aaatgttggg gatgtattag tagaaatcga tgctcctgga
     4801 cacaatagtg caccgtcaac tagtgcgcca tcagctgaag caccaaaaga aaaagtggaa
     4861 acatcaggtt cagcaagtgt cgttgaagct gccgatccaa ataaacgtgt attggctatg
     4921 ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata tctcacaagt aactgcaact
     4981 ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact tcttggcagg tggtccaagc
     5041 agtgcaccag cgaaatcaga agcgccagaa gcggcagcac cgaaagaagc agctcctgca
     5101 gcagaaagca aaccagctgc accagcaaaa ccattcaagt ccaacttagg agacttggaa
     5161 gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta aagcaatggt caacagtaag
     5221 catacagctc cacacgtgac attgcatgat gaagtagaag tgtctaaact atgggataat
     5281 cggaaacgat ttaaagaagt tgctgctgca aatggtacga aacttacttt cttgccatat
     5341 gtcgtgaaag ctttaacagc gactgttaag aaatacccag tattgaatgc atctatcgac
     5401 gatgcaaacc aagaaatcgt ttataaacat tactacaata tcggtattgc aactgatacg
     5461 gatcacggac tatatgtacc aaatgtgaaa gatgcagacc gcaaaggaat gtttgctatt
     5521 gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg gcaaactatc tgcagaagat
     5581 atgcgcaatg gaacaattac gatcagcaat atcggatctg taggtggcgg ttggtttaca
     5641 ccagtcatca actatccaga agtagctatt ttaggtgtag gaacgattgc acaacaacca
     5701 atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga tgaaactgtc attaagtttc
     5761 gatcaccgta tcgtagatgg agcaactgcg caacaagcaa tgaataatat caaacgtctt
     5821 ttagctgatc cagagctatt aatgatggaa ggatgataaa caaatggtag ttggagattt
     5881 tgctgtagaa ttagatacag ttgtaattgg tgcgggacct ggtggctatg tagctgccat
     5941 ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa agagagtata ttggcggtgt
     6001 ttgtttgaat gtcggctgta ttccttcaaa agcactgata gctgcaggac accattatca
     6061 agagtcacta gattctgaaa tgtttggagt aactagtgaa aacgtcaaac tagattttgc
     6121 aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg ttgacttctg gtgttggttt
     6181 cctattaaaa aaacataagg ttgaaacaat cgaaggagaa gcgttcttcg tagatgatca
     6241 cacactacgc gtcattcatc cagattcagc tcaaacttat tcgttcaata acgcaatcat
     6301 cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa ttcggaggac gtgtgttaga
     6361 ttctactggc ggattagcat tgaaagaagt acctaagaaa tttgtgatca tcggtggcgg
     6421 tgtgattggt gccgaattgg gcggagcgta cgcaaactta ggagcagaag taacgattct
     6481 tgaaggctca ccacaaattt taccaactta cgaaaaagat ctagtaaaat tagtagaaga
     6541 tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca atggcgaaag aagcagtcga
     6601 taacggtgac agcgtgacag tgaaatatgc agtggacggg aaagaagaat ccgttactgc
     6661 tgattatgtg atggtcactg taggtcgtcg tccgaataca gatgatatgg gcttagaaca
     6721 agcgggtgtc gaagtaggag aacgaggatt gatcacggtg gataaacaag gacgtacgaa
     6781 cgtacctaac atctatgcca tcggtgatat cgttccaggg gcggcattag cacacaaagc
     6841 cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc aaaaaagtag ctgtagatta
     6901 taaagcaatg ccggcagtag ccttcactga tcctgaattg gcttctgtag gtatgacgat
     6961 caaagaagca aaagatgctg gtatcgaagc aaaagcatat aaattcccat tttcagggaa
     7021 cggtcgggca ttatcgcttg gtaaaacaga aggattcatc cgtctagtta cgacgatcga
     7081 agataacgtg cttattggcg cgcaaatcgg tggagtcggc gcaagtgata tggtctctga
     7141 attagcttta gcaatcgaat caggaatgaa tgctgaagat atcgccttga cgattcatcc
     7201 tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa ttagcattag gtttaccaat
     7261 ccatatttga tttgaataat acaggcgaaa cgcaactgaa aaatcagttg cgttttgttt
     7321 tttagaatta tcaataatat tattatgtaa acttaaaaat atgacaactt cttggtttat
     7381 taccacttcc tatgctacac tgaagaggaa tatgaaatag gagagttgaa gatgcaagaa
     7441 tatcagtatc cacttgattt agattggaca acagacgaaa tggtcattgt aatgaagatg
     7501 tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca atgaagcttt tttgaagact
     7561 tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg aaaaacgatt gggacgggaa
     7621 tttgaaaaag cttccggtta ttctttatat cgtacagtgc agcaagcaaa aaaaaatcca
     7681 aacaaaactt tgagaatgaa cgggtgagat aaatggaaca gatgattgat catatcaagt
     7741 cctggcttga agaagctggt gagaaaatca aatcttctca acaaacatta acaatctcgc
     7801 aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg aaacatccag aattttctaa
     7861 tcgaaaaaat ccattccttt gatccagagg cgaaaatatt ggcagaagaa gaagggtgta
     7921 atcaattatc ggatctcgat ggaagagtct ttatcatcga tcctattgat ggcaccttga
     7981 attttgtgat tgaaggggaa aatttttgta ttatgctcgc tgtttatgaa tccggcgttg
     8041 gaaagcttgg tttcatttat gacgtgatga gaaatgaact tttttggggt ggaaaaggaa
     8101 taggcgtgta taaaaatgat gagccattgc ctagaccagc aaataaatcc ttatcggaag
     8161 gcttacttgg tgtgaatagt tatcttttcg gtcataataa atatggaatc cgagaaattg
     8221 cggaaagcag catggggaca cgtatgtacg gatgcgctgg tcttgaacta gtggcaatct
     8281 tgaaaggaaa ccacattgct tatatttcaa accttagccc ttgggattat gcagcgggaa
     8341 atgtcttatt ggaagagttt ggtatggtaa caagcggtct ttttggtact cctttgacat
     8401 ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc atatgcagaa atcgcagacc
     8461 ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc acaaaaatga taaaaacgag
     8521 caaaagatta attttctcga aaaaatgaaa acattttact atatttcatg aaaaaagtct
     8581 gctataatta gcagtcgagt aaaatttgcc ctgtcataaa gaaccaataa aaaaggagtt
     8641 agttacattg aattatagaa acgatatccg taacgtcgca attattgccc acgttgacca
     8701 cggaaaaaca acattagttg atgaactatt aaaacaatca gatacattag acgcacatac
     8761 gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa aaagaacgtg gtatcacgat
     8821 tttagccaaa aacacagctg tagactataa aggtattcgc gtaaacatca tggatactcc
     8881 aggacacgcg gacttcggtg gcgaagtaga acggatcatg aaaatggttg acggcgttgt
     8941 gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca cgttttgtat tgaaaaaagc
     9001 actggaacaa catatcacac caatcgttgt cgtaaacaag attgacaaac cttctgctcg
     9061 tcctgagcat gtcgtagatg aagttcttga actattcatc gaattaggag cagatgatga
     9121 tcagttagac tttcctgtga tttatgcatc tgcactaaat ggtacgtcaa gcttgtcaga
     9181 tgatccagca gatcaagaac caaccatggc acctattttt gatacaatta ttgaaaaaat
     9241 ccctgctcct gtcgataaca gcgacgaacc attacaattc caagtttcat tacttgacta
     9301 caacgattat gtaggtcgta tcggaatcgg acgtgtattt agaggaacaa tcaaagtagg
     9361 agaccaagta gcattaatta aattagacgg aacagttaag aaattccgtg taacgaaatt
     9421 atttggtttc tttggactaa aacgtctaga gattcaagaa gcaaaagctg gagacttgat
     9481 tgctgtctca ggtatggaag atatctttgt cggtgaaaca gttactcctg tggatcatca
     9541 agatgcgtta cctatccttc acattgacga accaacattg caaatgactt tcttagttaa
     9601 taattcacca tttgccggtc gagaaggtaa attcgtaacc gctcgtaaga ttgaagaacg
     9661 tttgatggca gaacttcaaa cagatgtatc cttacgagtt gaaccaacaa actctccaga
     9721 tgcatggaca gtttctggtc gtggagaatt gcatttgtct atcttgattg aaaacatgcg
     9781 ccgtgaggga tacgaattgc aagtatctcg tccagaagtt attgaaaaag aaatcgacgg
     9841 tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca ccagaagaat atatgggtag
     9901 tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa gatatggtcc atacaggaaa
     9961 cggacaaatc cgtttgacat tcttaacacc tgcccgcggt ttgattggtt attcaactga
    10021 attcctttca atgacacgtg gttacggaat catgaaccat acttttgacc aatatctgcc
    10081 aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg cttgtttcga tcgatacagg
    10141 aaaagcaacg acttattcta tcatgagtat cgaagaacgc ggtacagtct tcgtagaacc
    10201 aggtacagaa gtatatgaag gaatgattat cggggaaaac agccgtgata atgatttgac
    10261 tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg tctgctacta aagaccaaac
    10321 atctgtcatc aaaaaaccaa aacaattgac gttggaagaa tctttggaat tcttgaatga
    10381 tgatgaatat tgcgaagtaa caccagaaag cattcgcttg agaaaacaaa ttttagagaa
    10441 aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct gtaaaaggat gaagagagaa
    10501 accagcttgt tataaaaaat agctggtttc tttttttgtc caactctttt ctgtggagaa
    10561 gtcatctttg tcaatggaca taaattcaga aaatacgccg aaagacgttt aaaagacttt
    10621 taaaaaactt atttctagag agtttttgtt tcataataag ataatgtttc acgaaaggag
    10681 atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa gagaaaggag cgggaagatg
    10741 aacgacttat taaaacaata ctttggctat gatgagttta gacctggtca aaaagaaatt
    10801 atccagaaag tcattgatca ggaaaatgta ttaggaatca tgcctaccgg aagtgggaaa
    10861 tcaatctgtt accagcttcc agctttacta ttagatgggc ttactgtggt tgtgtcccct
    10921 ttaatctctt tgatgaagga tcaggtggac gcggcgaatc aattaggtat acccgccact
    10981 tttatcaata gctcgttaga tggttacgaa acagctagac gcttccaaga aatcgataga
    11041 cagcagtacc gtttacttta tatcgctcct gaacgtttta tcatgccgga ttttatccaa
    11101 gcaatgaagc gatggaacgt ccgtatgatc gctattgacg aggcacattg tatctctcag
    11161 tgggggcatg atttccgccc aagttattta cagatggcga accagctaga tcaactgcca
    11221 aatcgtcctg taattgttgc tttgacagct acagcgactg tgcaagtagc tgctgacata
    11281 aaaagacttt taaaaattcc agaaaacaac catattcaga ctggttttga acgagaaaat
    11341 ctacgatttc aagtaattaa agatcaaaaa aaagaacaat atctaatcga atatttaaag
    11401 atcaacaaaa atcaatctgg tatcatttat gcagcaacga gaaaagaagt agaccgtctc
    11461 tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat atcatggtgg tctaaatgag
    11521 aatgagcgaa cagaaatgca agaagccttt ttatacgatc gcttgcagct gattgtagcg
    11581 acaaatgcat ttggtatggg tataaataaa agcaatgtcc gttttgttat ccattatcaa
    11641 attcctggct ctctagaagc atactatcag gaagctggtc gggcgggaag agatggtctt
    11701 tccagtgaag ccatcctgct tttctctcct caagatatcc aagtccaaaa gttttttatc
    11761 cagcagtccc aaagagaaga agggcaaaag caaaaagaat atgaaaaatt aaaagcgatg
    11821 acagaatacg tgtatattga gtcctgtctt cagcaataca tcctaaacta ttttggcgag
    11881 acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg acagagaaat agtagaagta
    11941 acgacagaag cacagatggt tctttcttgc ttaaaacgta tgggtgagaa ctatggtaaa
    12001 caaatgctga tgaaagtctt agcaggatcg aaagaacaaa agctgcaagc tttaggtttt
    12061 ggacatttat ctacctatgg actgatgaaa aaccaatcgc aaaaagaaac gatgcagtta
    12121 attgaatatt tgatatcgaa tggctatctt ctgaccatca atggtgagta tcctgttttg
    12181 aaagtaacag aaagagggat acaagtattg aaaggacaag aatctgttta tcgaaaggaa
    12241 cccaagaaag tccagcagct atcggatgaa gaaaccgata cactatttga agtgctgcgg
    12301 gaactgcgaa cggatctagc atcagaagca ggcgttcccc cgtatgtcgt attttcagat
    12361 tccacattga aagaaatgag ccgaatacgt ccatcaagca gattggagat gcttcagata
    12421 aaaggagttg gccaaagcaa gcttgataaa tatggcgaag cctttttatc tcggataaaa
    12481 aatgcggact ctaacgttct ggataagtag aaagaacgaa tacgtaaaaa tgaataaaaa
    12541 caactggtta aatctgtttt ttttgctcct tatgaaaatc aaatatgtta taattaagga
    12601 attagaatgg gaggtaagca cagatggatt ctttgtcgaa aaaacttgcg atccaagtat
    12661 taaatgatga agcagaacgg atcaaacgat taatccgaaa tcaaaacaac agtctctgta
    12721 tctcacaatg caaagccttc gaagaagtgg tagatacaca gatgtacggc ttttcccggc
    12781 aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa cgagggccag ctgatgttaa
    12841 gtgagctaga acgagaattg aatcagttat atagtgaggt ttaccaagaa atttatgata
    12901 aaaaagaagt tggcaaggag gaatagactt gcctaagaaa gtgaaaaaaa ggcatctatt
    12961 agactatagc atcttgattc cctatttaat tttatgtgta ttgggattga tcatggtata
    13021 tagttctacg tcgtatctat tattagaaaa cggacaaaat ccgtccgcat cagtcattaa
    13081 ccagagtatt ttttgggtgc ttagcttgat tgctatcgca ctactttata aaatgaaaac
    13141 agatgttttg aaaaaccagc gtttgatcat ggcagccatc gcagttttga cgattttgct
    13201 gctgattgtt gtgttttttg gtaaggagat caatggggcg aaaggttggc tgcaaattgc
    13261 aggattctcg atccaacctg ccgaatacct aaaaatcatt tctatctggt acctgtctct
    13321 gactttatct aaaagacaaa acagtgtaca aaaagatttt cttgggactg tgaaacgtcc
    13381 acttgcaatg gttatcggac ttactgcatt agtcgctatt cttcccgatt ttggtaatgc
    13441 ggcagttatc tttttgatta ttttggttct gcttttagcc agcggagtca attatgtgta
    13501 tactttgatt gtaggagtag gaggcttttg tctaagtact tttacgatct ggctgatcaa
    13561 tatcacaaac ggaaagatct tacccggacg tttacagtat atctataatc gatttgctat
    13621 ttaccaaaat cctttctcag acgaattaaa taaagggcat caactagtca acggctacta
    13681 tgccatgttc aacggaggac tatttggcag gggattaggt aacagtatcc agaaaaaagg
    13741 atttttacaa gaagcacaaa ccgattttat ttatgcaatc gtagtggaag aactgggagt
    13801 gattatgggg attttgatat tagcgttgct attctttatg attgtgcgca ttgtccttgt
    13861 aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc attgggatcg gcgcaatgtt
    13921 tttgatccaa gtatttgtca atcttggagg aattactggc gttattcctt tgacagggat
    13981 cacgttccca tttttaagtc aaggaggctc cagtttgctg atgctttcga tttgtgtcgg
    14041 ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc ttaggtttat aataaaaaaa
    14101 gatgtaggag tggttcgatg aaaaaagttt tagttgctaa caggggtgaa atcgctgtac
    14161 gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt tggaatctat gcagaagaag
    14221 atgaatattc cgtacaccgt ttcaaagcgg atgaagcata tttagtagga caaggaaaaa
    14281 aaccgattga tgcgtatcta gatattgaag ggatcatttc tatcgcgaaa gaatgcggag
    14341 ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa cttgaatttt gcgcaacggt
    14401 gcgaagaaga aggcatcatt tttgttggac ccaaactgca tcatttagat atttttggag
    14461 ataaaatcaa agcaaaagct gcagctatcg aagcggggat tgcttcgatt ccaggcacag
    14521 atggaccaat cgcttctata gacgatgcct tggagtttgc gaaacaatac ggttatccga
    14581 ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat gcgtgtagct cacgatgaaa
    14641 agagtgcaag agagggctac gaacgtgcga aaagcgaagc aaaagcagcc tttggtagtg
    14701 atgaagtgta tgttgaaaaa tacatcgcta atcctaagca tatcgaagta cagattctcg
    14761 gggatacgca tggaaacgtc atccatcttt ttgaaagaga ttgttctgtt cagcgccgtc
    14821 accaaaaagt agtagaagta gcgccttgtg tctctatgaa tgaacagcaa cgtcaaaaaa
    14881 tctgccaggc agctgtccaa ttgatgaaat atgtcgggta tgtcaacgca ggtacagtag
    14941 aatttcttgt tgaaggagat gatttttact ttatcgaagt caaccctcgc gtccaagtgg
    15001 aacatacgat cacagaaatg attacagata ttgatatcgt aacaacgcaa ctgttgattg
    15061 ctcaaggttt ggatctgcat aaagaaatcg gattgcctca gcaagaagga attaaattaa
    15121 atggttcagc cattcaatgc cgaatcacta cagaagaccc gttgaataac tttttaccag
    15181 acacagggaa aatcgatacg tatcgttcac ctggtggttt tggcgtacgt ctggatgtcg
    15241 gaaatgctta cgcgggctat gtagtgacac cgtattttga ttcattgcta gtaaaagtat
    15301 gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat ggaacgttgc ttgaaagaat
    15361 ttagaatccg tggagtgaaa acaaacattc catttatgct caatgtcatc acacatcctg
    15421 aattccaatc aggaaatgca aaaacaacct tcattgatag tacagcaaca ttatttgaat
    15481 ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata tatcggtgaa attactgtga
    15541 atggttttcc aggtatcgaa agtggcgaaa aaccatttta tgaagaacca cgtatgccta
    15601 aagatctaat cacaagaact gattacgtaa cggctaagaa tgtattagat gcgaagggtg
    15661 ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt gttgttgacg gatacgacat
    15721 ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg gacaaaggat ttcaagcaga
    15781 ttgcacgttt gactggagaa ggtcttcctg aactcttttc aagtgagatg tgggggggag
    15841 caacatttga tgtggcttac cgctttttga atgaagatcc ttggcaacgg ttgagaaaaa
    15901 ttcgttcttt aatgcctaat acgttgctgc aaatgctttt cagaggttca aatgccgttg
    15961 ggtattctaa ttatcctgac aatgtattgg tggaatttgt caaagaagca gcagcgcaag
    16021 gaatcgatgt atttcgaatc tttgatagtt tgaactggac tccacaaatg gaaaaaagta
    16081 tccaagctgt acgggatact ggtaaaatcg cagaggcagc tatttgctat actggcgata
    16141 tcaacgatcc aagtcgggca aaatacaatg ttcagtatta taaagacatg gcaaaggaac
    16201 tagaacaact gggtgcgcat atcattgcaa tcaaagatat ggctgggctg cttaaaccgc
    16261 aggcagctta tcgtttgatt agtgaattga aagaaacgac agatctgcca atccatctgc
    16321 atactcatga taccagcggg aatggaatca tcacttattc tgctgcttca aaagcaggag
    16381 tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa tactagtcag cctagcatga
    16441 gcagcttgta ttatgcactg gtcaatggtc cacggctgcc agaaatcacg attgaaaatg
    16501 cacagaaatt aaaccattat tgggaagatg tgcgcatgta ttacaaaccg tttgaaaacg
    16561 gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat gccaggagga cagtattcga
    16621 atctacagca acaggcaaaa gctgtcggat taggtcatcg ctgggatgaa atcaaacaaa
    16681 tgtatcatac tgtcaacttg atgtttggcg atatcgttaa agtaactcca tcatctaaag
    16741 tagttggaga tatggcacta tttatggtcc aaaatgatct tacagaagaa gatatttatg
    16801 aaaaaggcga aacactaagt ttccctgaat ctgttgtgac tttcttccaa ggagaactag
    16861 ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat tattcttaaa ggcagacctg
    16921 cattgaatga acgtccaggt cttttggctg aatcagtcga cttcaacgaa gtaaaaaaag
    16981 aattggcaga aaaaattggc tatgaaccaa aacaagacga agtattaagc tatttgatgt
    17041 atccgcaagt ctttttggac tatcaaaaag catataatca atttggcgat gttacattac
    17101 tggacacacc aacatttttc caaggtatac gtttgggtga aacaatcaat gtacaaatcg
    17161 aacgcggaaa aacgcttatc attcgtttag atgaaatcgg agaacccgat atcgaaggga
    17221 atcgcgtact gttcttcaat ttgaatggac aaagacgtga aatcactatc aaagataatt
    17281 cgatcatcag tacggtccaa acccgattga aagctgaacc gacaaaccga gaacaaattg
    17341 gagcaacgat gtctggttct gttttggatg tattagtgaa aaaaggcgat aacgttaaaa
    17401 aaggtgatac gttgatgatt actgaagcga tgaaaatgga aacggccatc gaagcacgtt
    17461 ttgacggaga agtcgcacat gtatatgtgt cttcaggaga cactatttcg tctggtgacc
    17521 ttttgattga agtgactgag aaataataag cgaaaggtgg ggaaacatga aaagactggg
    17581 tttgttttgg ctgattctgc tattagttat tgtggcattc tacatggaac cagtcttgtt
    17641 tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa atatcaaacc aaatgacaac
    17701 gtggaaatac caggagctta aagcaactgg tttttctact tatatcggaa aaccgatcga
    17761 tcagctggaa aaggattttg gccaagccta tgatcgatta actagcggat ttggttttga
    17821 aacaagatat tatgaaaata aagctagtca tctcacattt gaagcgaaca tccaagacag
    17881 cagagtcaca actgttaaag tactgcaaac agggcctgct gatatcgcgc cgttttattt
    17941 agggatgacg atgcaagatc ttacgaaaat cactactatc tatactaatt tcacttttga
    18001 gtaccatgat acagaagttg gaatcgaact aatggaagag gatatgaatt atcgtccgct
    18061 aattgctttt gataacgaca cttttgctat tttatttttt gatcaaacaa caaacggcct
    18121 attttcagtt gtgtatttag ataaagatag tcttttgaaa ctattgccat atcaagtgtt
    18181 tggtgaaggc cttccacatt atcaagcaga taaatcggct gattgggaat caatcaatcg
    18241 cgttaaagaa cgaaaaagta cgactttatt aaatatgctt agaagagaag acgaacttcc
    18301 catatacaat caaacacttg cctttcaaaa ccacagtcag ctgctactac atgatttttt
    18361 gacaaggcct gaggaaatat tgtcagagga acgtatggct gattggcaaa aatcgctagg
    18421 cagtgctcaa acgacagtga aattctcact gacgaatgat gaattggaga agttaatcag
    18481 taaacaaaaa ttagaaaata ctagtgggct atttattcat ccggcgattg acgctacttt
    18541 ttcgttttta tattggtaca gcgatcctta caaccatgac cgtttcatgg acccgggaac
    18601 ggattctgta ggcattgctt tttctaaaga aaatatgtta gtcttattgc aagaagaaaa
    18661 cggagaaagt agtgatagca gtgatcgtta acgaagagct gtttcaactg gaggatcaat
    18721 gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat ggcttcttat cttgaacata
    18781 aacagtcaat ggaagaatct gcaaaagttt gccagttgaa agaagcgttc ttgtctaaga
    18841 aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc acctgattat cgtgaaaaaa
    18901 aacgagctgt tcgaaaagca aaaagacagc ttgatatgga tgagcatgtt gcagctttcc
    18961 gatatagtga aacacagctg caaagtctgt tggatcaaat tgtccaagta cttgccgaag
    19021 aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt ttttgaaacg aaaaaacact
    19081 ttggatgcgg aggtaattgt catgcaatta gacaatagtg aatttgaaat aacaaaacga
    19141 cgcgggctga tcgtatgggt ctacagctta aaacaattaa agaatctgaa aaagttcggc
    19201 ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc tttacttaaa tgaagagaca
    19261 ttggaagaaa cagaagaacg aatccgtaaa ttacattttg ttcgaaacgt agaacgttct
    19321 taccgaccgg atatcgaaat gaattttgcc gaaaaaatag gaaacaaaac agtattaaaa
    19381 gacgaaggtt tcgaaattga agagctaaat acagaaatcc gattagctga acatctttaa
    19441 aaatctctta tgagcagttt ttaaataaaa aataacttgg ataacgatcg atttttgatc
    19501 attgtccaag tatttttttg gtatactagt accagtacca tttctaagga gtgagtagat
    19561 gcggataata tcaggaaatt atggcgggcg ccgtttaaaa agtttagcag gggcgaacac
    19621 cagaccaact acagataaag taaaagaatc catattcaat atgattggac catattttga
    19681 tggagagaca gtattggatc tgtttgcagg aagcggtggt ttagctatcg aagcagtatc
    19741 tagaggctgc agtcatgcgg tatgcgtaga caaaaattac caagcgctaa aaatcatcaa
    19801 agaaaacatc gaaattacga aagaaccaga gaaatttacc acaataaaac ttgatgcgga
    19861 caaagcaatc gaacggctag cagcggagaa acaacaattc gactacttgt ttcttgatcc
    19921 gccatatgca aaacaaaaaa taatcgatca aatccataaa atggaagaac tgggactttt
    19981 ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca gtcgaattgc cagaaactgt
    20041 ggctggattc catcaaataa gaaaacagat ttatggcata acggccgtaa ctatctatag
    20101 aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa gttttgatcc ttttacaaaa
    20161 ggtcacttag atacggtgga acgtgcggct aaattattcg atgaggtagt aattggtgtt
    20221 tttatcaata cttcaaagaa aagtttgttt cccccagaag aaagaatgac attgatcaca
    20281 aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc aagaaaatca attgacggta
    20341 gaaacagcaa aagagattgg tgcagatgcg ttgatcagag gcatacgaag tatcaaagac
    20401 tttgaatatg aacgagagat tgctcagatg aatcatcatc tatatgatga attggaaact
    20461 gtcttcttat tagcaaaacc agaatatagt cacgtgagtt ccagtatttt aaaagaagta
    20521 ctgcactttg gcggagatgt gagcagctac ttaccgccag tgatcaacga agctttagct
    20581 ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa acagtactga ttatcatatt
    20641 tggaatgttg atcattggat ttgtagtagt tcccttgccg tattatgtag aatcaccggg
    20701 agccacgatc gatctgaaag aactaattac ggtcaatgat aaggaagata aaggttctgg
    20761 gtctttctct cttacctctg ttggtatcag gcaagctaca ggtttcttag ctttaaaagc
    20821 taaaatgacc ccttttgaag aattgatcag tgaagaagaa ttgactggtg gggcgacaaa
    20881 tgaagaatat aatcaaatgc aacagtatta tatggattct tcccaaaatg cagcaatcga
    20941 acaagcgtta aaacttgcag acaagcctta taaaatggaa ttcaaaggcg tatatgtcat
    21001 gagtgtagag aaaacatcga atttctttgg caagctttca gttggagata cagtaacaaa
    21061 agtagacggg aaaagctttc aaagtacaga agcatttatg gattatgtaa aaagccaaaa
    21121 agttggacaa acaatcgaaa tcagttattt gcataatggt gaagaaaaaa cagcttctgg
    21181 tgatctgatc gagttaccaa cagataaaaa ggcaggaata ggtatcacgc ttactgacca
    21241 taccgagatc gaatcagata cagatgttcg tattaattct ggctctatcg gtgggccatc
    21301 agcgggattg atgttcactt tagaaatcta tgaacaagtc actggtaaaa acttgcgaca
    21361 tggtaagcaa attgcaggaa ctggaacgat caacagtgaa ggagaagttg gccgaatcgg
    21421 aggaatcgat aaaaaagtgg caagtgcgga taaagctggc gtagagattt tctttgcacc
    21481 agatgatacg atttcagacg atgttaagaa agaatatcct gaaataaaaa ccaactacga
    21541 agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg aaaatcgtac cagtaaaaac
    21601 agtacaagaa gcgcttgact atctagagaa aatgaaatga aactaaataa ttaaagaggg
    21661 gttgtgacac gagttctgtc acaacccctc tttttcgtca aaaatcttca cgataaaaat
    21721 aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg gacgattttg ccagagctca
    21781 ctgtttcttc aattttaaat gaaaggagta atgaatgatt tttactggtc attctttgaa
    21841 aaaagaacaa tgatagaaaa aatcagcaag aaaatactag cttcacccaa attgatcatt
    21901 tggatgtcta tgatagttct tatcttttgt atcgcaagtg gttttttatt atcaaatttg
    21961 ctattttata gagaaacaga agagactgct gcaaaagaaa tactttcttc gtttagttcc
    22021 agtggagaag atccatcagc tgaaggaaac gatgtagaag aggatgctcg tccaaaggtc
    22081 atgtacaccg atatcaaagg aagtgtgaag gagcctggta tctattcctt ttcttcagaa
    22141 gaacgggtct atgatgtact aaagcgtgca ggtggattat tagaagaagc ggatagcgat
    22201 cgaatcaact tttctgcgaa gatagaagat cagcaagtcc tatatatccc tgctgtaggt
    22261 gaagagctgc cagaacatct tgatcagttg gcatcctcgg aaggaaaaca gcctacagca
    22321 gattccgagc ctcccaaaat aaatatcaat acagccactc catcagaact tcagcagatt
    22381 cctgggatcg gatcagtcaa agcgcaagaa atcattcgat ttagagaaga aaacggatcc
    22441 ttccagaaag tggaagacct gcaggagatt tctggtatcg gtgaaaagac agttgaaaaa
    22501 ctaaaaaact tcgttacaat taaatagaat gattcaaaat cgactggcac gagcaataaa
    22561 tggacgctaa tggatactaa gaataatcca attctaagag cttggcagga cgaggaggta
    22621 gtatttttgg ataggaaata gcttgattgt tcgcaaagaa acattaaaaa aaccaatgct
    22681 gaatgtctat ctccaaaata aaatatctgg gattcatatt atgaatacgg ctgtcagcgg
    22741 caataacagt caagctctac gagaacgctt tgccaaagat gtacttagct atacagcaga
    22801 taaagtattt attctcattg ggacgaatga tctagcagag cataaacaac tttctaaaga
    22861 aacgtaccaa aaaatctgca gtggatgata gttcagctga aaaaagacta tagttctgat
    22921 catgctttct ttatcacgcc accagcagtc gatgagacaa aacaaaaata tcggaataac
    22981 aagttgatca aagaatatat ctctgttctc acagaagtag caaaacgcca tgattgtgaa
    23041 gtatcgattt ttatacggaa tttcttgaac aaatggattc gaagaatttt tataatgggc
    23101 taatggatga cggacttcat ttcggcagtg aagggtagga tcttttgtca gacaggatga
    23161 ttcgttatat ttaaagaata ttttgtttaa gcaatgagta ctttttaatc aacttgaaaa
    23221 aacggaggaa tataaaacat gttagaaaaa tcttcaaaag catgcgatac gaataaagaa
    23281 aaagttactg ataagtcaca taatgaacgc attccttggg accagtactt tatggctcag
    23341 gcagtactgt tatctttacg tagtacgtgt acacgtttag aagtaggagc gacactcgtt
    23401 cgagaaaaaa ggatcattgc tggcggctat aatggagcag tcagtggaga tgtccactgc
    23461 atagatgaag ggtgttacgt cgtggatgga cactgcttgc gaacgatcca tgcagagatg
    23521 aatgcactcc tacagtgtgc aaaacttggt atcccgacgg aaggttcaga aatctatgtc
    23581 acacattttc catgtttggc ctgtacaaag gctttgttgc aagctggtgt gaaagggatc
    23641 aattatctcc atgactatcg aaatgatcct tatgcccaag cgttgatcga acaattaggt
    23701 gtatctgtac accaagttgc tttagactca cagcattttt caaaacttca aaatgaatta
    23761 acaggaaaac atgtctagcg ttttagaaag gcatgttcga aatcggttga tttttccggc
    23821 aattacgtcg atgattatct gtttgttgta ttacactgac aattggggtt atcgtttgct
    23881 tcttgccgga atttttttat ggttttcgct aaaagaagga cgttttatcg cgtacttgtg
    23941 tattttgtgt agcataacgg tatccatttc ttataggcta caagagtatt catttttaga
    24001 ggaaaaaaca acatctgcgc aaattactgt atttacagat acgattcgtg caaatggtaa
    24061 ttttttgaca atggaaggaa ggttatcaga gaataaacaa aagctccagc tttcttatca
    24121 agtaaacaca gaagcagaat tgaaaacatg gcttaacttc cgaggaaagg gaatagaatt
    24181 gactgctgaa ggcgagttca tctctccagc aaaaaagcgg aatctttata attttgacca
    24241 agaagcttac ttcaggaacc atagaatttc tggacggttc caaattcaaa agattgaaca
    24301 gatcaccatg caaaatcact ggcgaaactg gcttcagaga aaaagaggat cttttatctc
    24361 ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat attaattctt tattatgggg
    24421 ctataaagat gcaggattca gagaaccaga agaaattttt agggagagtg gtttgctgca
    24481 tctgttcagt ctatcgggtc tacacatcca attttattta gggtggctgt attatttgtt
    24541 tcgacgtatc ggatggccac tacacgtcag tattattccg ttgagtctac taactatctg
    24601 ttatgtttct attgcaggaa gttctatttc tgttttgcgt gctagtttgc tgtttttatt
    24661 gcgattgcta gtcaaattgc tgaataagca ttattccacc atggacttat ttgcagttgt
    24721 cttttggctt cttttactat ttgatcctcg atttcttttt catgcgggtg gacagcttag
    24781 ttttttcatg agttttttgt ttatcctgat ttcttttgat ggagattggt tgaaaaaaac
    24841 ggcctctcac gtcctgttta ctatgatcac gatgccgttg attgtatggc atttttatga
    24901 gtggcctatt ttaggtagtc tttttacatt actgattgca ccggtattca agtttctttt
    24961 tcttcctggt gtgttatttc tcacactctt caatcgctgg ctgccggaaa ctttactttt
    25021 gctgatagaa gagggattaa tcttatttga gcgaataatc gatttttctt cagggtcgtc
    25081 tctcattata ggacgtttgc cccttgtgct atctgggatg tatattttcg gattactgat
    25141 ttgtatggat aggttgaaag aacaacctgt gaaacacttg ttttatgttg gtcttttttc
    25201 ttttattttt ttgactttgc catttttacg cttttcttca acgatcgctt ttatcgatgt
    25261 cggtcaagga gacagtatct ttttgcaaag tccttttcga aaagaaacga ttttgatcga
    25321 tactggaggt cagttacact ttgaaagaga aaagtgggcg caaggtatga tacgtcctcc
    25381 tgctgaaaga aatatcgttc catatctaaa aggtcagggg ataagtgtaa tcgataaact
    25441 catcttgaca cacgacgata cggatcatgt gggagaatta cccacgttat ctcagcattt
    25501 tactatcaaa aaaatttata tcggttgggg cgctggacga aatgagccat tacaaaggca
    25561 tttatctgaa gctcaaaaaa atggtactga gatcattgaa atcaaacatg gggacaggat
    25621 cagcggttat tatgatcttt atgtattgac tccttttgaa aagggagagg gaagaaatga
    25681 agattcgatt ggtctatgga tggaatataa caataggcgc tttttatttt taggagattt
    25741 gaatcaagca atggaaaaac aattgctttt gatttatcct aatctgaaag ccgatgtagt
    25801 caaattaggt catcacggta gccgtacgtc aagtaacaca gattttcttt cccatattga
    25861 agcaaaacat gggattatat cctgcggcgt aaataatcgt tatggtcacc cgcacagcga
    25921 agtattggag acactagaag aaaatcagat aaaaacatgg cgaacggatc aacaaggaat
    25981 gatcacctat cgctggcatc cagtttttca tcctcatggg ctagtggaaa caatgataga
    26041 ctagtaattc tcatctgctc atggtaggat aatttttgaa agtggtgagt aatttgagta
    26101 tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc atcttgttac ttgattttag
    26161 gaacagaaaa gtttctccaa gatcaagttc ggacagaaat tgtcaaaaaa atccaattag
    26221 agggcgaaga tgacttaaat tttttaagtt ttgacatgga aaatgcaagt ttagatgagg
    26281 tagtagctga agcagagacg ttgccgtttt tcggagatca acggttagta ttcgtagaga
    26341 atccttattt tttaacaggt gaaaaagtaa acaatggaat cgaacaaaat actgatttat
    26401 tgacggctta tttgaaagaa ccattagaat cgactgtatt agtattcttt gcgccatatg
    26461 aaaagttgga tgaacggaaa aaagtaacga aacaattgaa aaaaacagca attatcatca
    26521 atgtccaaca gttgaatgaa aaagaagtac gtcaatatct gatgaatacc ttagagaatt
    26581 cctcaatcaa aatggaacga agtgcaattg atttattctt gaggttgacc gacttagatt
    26641 tatccaaaat gatgagagag ttacagaaat tggtccttta tggacaaaat caacagcaaa
    26701 tcacggtaag agaagtagaa gaattagttc ctaaaacgtt agaacataat atttttgaca
    26761 tgacccaata tattttaaaa gggaagacgg aacaagcttt gcgtttatac gaagatttag
    26821 ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt gctttctcaa ttacgtttat
    26881 ttttacagac aaaatttctt gtaaagattg gttatcagca ggcaaatatt gctgaaacat
    26941 tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga agtaaaaaaa ttcgatgaac
    27001 atttgctggt acagttgttt gatcaattag ttgagatgga ttatcaaatc aaaacgggac
    27061 aaatcgaaaa agaattaagt tttcaactat tcgttttgcg cacagggcag ttgttaagaa
    27121 atcgataaga agaaagacga gggcaaagca tgcgggaagg tcgtttgttt ttgtcctttt
    27181 tttgacaaat aaaaaaatcg gccgaagccg attcttttat ttagcgattt ttttgcttaa
    27241 acgagatttg tcgcggttag ctttgttttt gtggattaga ccttttgatt cagccatatc
    27301 aatagcttta acagcttctt tatataacgc atctacgttg tcagcacctg aagctacagc
    27361 atcttcaaat tttttgattg cagtacgcat agcgcttgtt tgagatgaat ttttcacatt
    27421 agcgttttcg cttgtacgaa cacgtttgat tgcagattca atgtttggca ttagtttcac
    27481 ctccgataac ttaagttctt acgcgtgtta ccgcataatt caacattcat tattatacct
    27541 aaaggcagcg tgctttgcaa tatgaacatg acaagttttt ttccttaaca gtcttttatt
    27601 aggatttttt ttcagtaaaa acggatagta caagctgaaa aaaatcaaaa agacgctatt
    27661 gtgggaggca atagcgtcaa accaaactag atttttattt atctatttaa acaaagtttg
    27721 aatgggaatg tttggtacta agttattata taacaaagag aaccgtttgc aaagtttttt
    27781 ttgaaaaggg atacgattcc ctgttgttct ttgtttttct attaagaaac tgcagttatt
    27841 ttataaccag tatcctttga tcagtaaagg ctattctggt gtaccgatta ttttaatgta
    27901 ttcatcgata ttttttatca acattttttt tatgaccaac tggttccgac ggttccattt
    27961 gccacttact tccagcacat atttatttag gggcaaacgt aagatatatt ttgccaaata
    28021 gcgatcttta agcaggccat tgatcacacg gtcctcaagc tgcaagttga tcccagtcag
    28081 catcattggc tgttcttgta aaacgtacgt atcaattaaa agaccttcaa aagtttccac
    28141 aagcgtcttc ctttcttttc aaagtaacct gaaaacaggc actaattctt ccattttgat
    28201 attcgttgaa gtggatgcga tcattctttc ttttgtccac tgtaatctag tataacaaaa
    28261 aagagcagaa gagccaaaaa gaaaaaagag catcgaattc tataggaagt ctcattggca
    28321 cggcctaaat tattacaaaa cattgattaa aaaaagcccc tgcatcgcag gagcatcccc
    28381 tatatcgttt taataggtca atcaaaaagc agtatcaaac acattttgta tcaaatcagg
    28441 aaataataga taagctaagc ccagaatcaa aagtaccgct agaagaaacc agccgattgt
    28501 tcctatgaaa tactttaatt tttcgaacat tgtatcacct cgttgtcagt atatcacgga
    28561 tcagtaaatt gggggtgtta agaatctcct aaaatagaca aaatttgttc tattttccta
    28621 aagcaaatac ttgtaagata cagtaagtga taccagaagc aatgaccggt ccggcggcga
    28681 ttcctcttaa gaagacaaca cctaaaatcg tgccgaaaac aagtgcaaca gtgatttgtg
    28741 gatcagaagc aatcaatcct acacccttgg aagataagac agccactagc ccaccgcaaa
    28801 aaacagctac ccagccaata ggggatttaa aggcttctaa tagatcacgt agaccgattt
    28861 ctcctgttgc gatagggatt agaatagccg ctgtgatgat cgtcacccca atattgattc
    28921 ctttgccttg aagccaaggg aaaaaggaac gggaggctgg tatgagcttc ataatcaaaa
    28981 caaaagctgc cgctatcatc agactttgat ttttagctaa aattgctaca agtaaaataa
    29041 ttcctaaaaa aagccaactt tccatgaaat atattcctca ctttcttcga aacaatcata
    29101 acatgttttt agaaagttac aaaagtagga attcatcctt caagacctat tcataagctg
    29161 tttttttcat aaaataaaca aaatcataaa aaaacacttt gcttatttga cagaagacag
    29221 ccgcgctttg tatagtggac aagacttaca tttttttgaa gaatcagaaa gatcaggtaa
    29281 aggtcttagt aaacgcaagc cattcagaat aaccaatatt gtgcttcctt cgtgtcctac
    29341 aactccaaga ggtagattta aaaattgcag gaagttagaa gcaatcaaca agagaataac
    29401 aagaatcgaa aaaataatat tttgtttgat gatccgccgc atttttaaag atagccgatg
    29461 actgtagaca aatttattta agtcgctttt cattaagacg atgtctgcga tatccatagc
    29521 ggcatctgtc ccttgcccca tagcgacacc taaagaagcg gctgctaaag caggcgcatc
    29581 attgactcca tcaccaacca tcgcatttac ttggtaaagt tcattctctt ttttgactag
    29641 ttgtgttttt tcttccggag tacagcttgc atagtaatgt tcaatcccta cttgttcagc
    29701 aatggccttt gctgtgccag aatggtcgcc ggttatcata cttgtatgga tgttttggga
    29761 tttaaagtag gaaattactt gttgggtatt tgcttttgga atatctagca gacctaaaac
    29821 agcaattaac tgctgatctt tagaaagata aatcacggtt ttgccttgat tttccaaacg
    29881 ttcgatcatt tccgcaatct ctttgctgat tatcatcgtc tcaggatcat atgcgtgctt
    29941 gccaactttc cattgcgcac ctaaataggt agtttgtaat ccaaaaccag taatttcttc
    30001 tattggtaag tattggattt cttcaggtat tgtgcaatcg aaatattgca aaatcgcatg
    30061 agcaagtgga tgcgtcgttt tttgttccat agcaacaaga agttgttgtg ccaaatcctt
    30121 ttcatcaaag aaaaagctgt cagttacaac tggttttcct ttagtgagcg ttcctgtttt
    30181 atcaaaggca atcgctttta attcagaaag cttttctaaa tggataccgc ctttgattaa
    30241 aatcccattt tttgcagaat ttgaaagagc agctagagaa gctggtgtag cagatgcgac
    30301 aagcgcacaa ggagaagcca caactaataa taccattcct cgataaaagc tttcatccca
    30361 actccaacct aggaaaaagt gggggagaag gatcattaga ggaacagcaa gcaaaatcaa
    30421 ttttacataa ttattttcga ttcgttcgat aaatgaagct gtttgagttg gcgtattctg
    30481 cgcttcttct actaattgga taatcttagc aaagacagtt tcggaactcg tttttgttac
    30541 tcgaatcgtg atgggatttc cttgattcag cgttcctgca tacatctcat cgcctaaata
    30601 tttttctaca ggcacagatt caccgcttaa tgcagactca tcgatcgttg cctgtgtgct
    30661 ttctaaaaag ccatctatcg gaacagctgc tcctttagcg acaaatacca agttatccac
    30721 ctttagttga gaaacatcta cttcttgcat ttgtccattt tcttttaata attgggcttt
    30781 ttgtggttga agattcatca aagcagtaat ttctttttga cttttattag ttgtgtattc
    30841 ctctaatgca ccgcttaaac aaaagatgaa tgtcaacatt gctccttcaa agtaattacc
    30901 gatcaaacag gcaccaatag cagctaaagc catcaataaa tccacattca gtgtccgatc
    30961 attccacaat tctttcaagc cttccgatgt ttgtttatat ccaccgatga taatggcact
    31021 gatataaaaa agtggggtga tggtaagaga gaatcggtcg aagataaaac cgaagatcat
    31081 aaataaaagg cagccgattg ttgctgcaaa agcttttttc tgattagtca tcgttattag
    31141 acctccttgt caaaacaagg atagcataat aaaaatgtaa ttgataataa taaca
//
LOCUS       DACCXW010000021        30025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCXW010000021 DACCXW010000000
VERSION     DACCXW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.021"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(183..761)
                     /gene="pgsA"
                     /locus_tag="IVY71_000759"
     CDS             complement(183..761)
                     /gene="pgsA"
                     /locus_tag="IVY71_000759"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7984829.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(970..1854)
                     /locus_tag="IVY71_000760"
     CDS             complement(970..1854)
                     /locus_tag="IVY71_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7984830.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(2632..3918)
                     /locus_tag="IVY71_000761"
     CDS             complement(2632..3918)
                     /locus_tag="IVY71_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7984831.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(3911..5179)
                     /locus_tag="IVY71_000762"
     CDS             complement(3911..5179)
                     /locus_tag="IVY71_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7984832.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            5431..5892
                     /gene="mscL"
                     /locus_tag="IVY71_000763"
     CDS             5431..5892
                     /gene="mscL"
                     /locus_tag="IVY71_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7984833.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5951..6397)
                     /locus_tag="IVY71_000764"
     CDS             complement(5951..6397)
                     /locus_tag="IVY71_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7984834.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(6490..6735)
                     /locus_tag="IVY71_000765"
     CDS             complement(6490..6735)
                     /locus_tag="IVY71_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984835.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(6850..7428)
                     /locus_tag="IVY71_000766"
     CDS             complement(6850..7428)
                     /locus_tag="IVY71_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7984836.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(7505..8035)
                     /locus_tag="IVY71_000767"
     CDS             complement(7505..8035)
                     /locus_tag="IVY71_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7984837.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(8039..8899)
                     /gene="rlmB"
                     /locus_tag="IVY71_000768"
     CDS             complement(8039..8899)
                     /gene="rlmB"
                     /locus_tag="IVY71_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7984838.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(8892..9287)
                     /locus_tag="IVY71_000769"
     CDS             complement(8892..9287)
                     /locus_tag="IVY71_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7984839.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(9284..10696)
                     /locus_tag="IVY71_000770"
     CDS             complement(9284..10696)
                     /locus_tag="IVY71_000770"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7984840.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(10693..11229)
                     /gene="cysE"
                     /locus_tag="IVY71_000771"
     CDS             complement(10693..11229)
                     /gene="cysE"
                     /locus_tag="IVY71_000771"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7984841.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(11551..13011)
                     /locus_tag="IVY71_000772"
     CDS             complement(11551..13011)
                     /locus_tag="IVY71_000772"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7984842.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(13181..14338)
                     /locus_tag="IVY71_000773"
     CDS             complement(13181..14338)
                     /locus_tag="IVY71_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7984843.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(14372..15742)
                     /gene="radA"
                     /locus_tag="IVY71_000774"
     CDS             complement(14372..15742)
                     /gene="radA"
                     /locus_tag="IVY71_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7984844.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(15805..16332)
                     /locus_tag="IVY71_000775"
     CDS             complement(15805..16332)
                     /locus_tag="IVY71_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7984845.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            16562..17368
                     /gene="proB"
                     /locus_tag="IVY71_000776"
     CDS             16562..17368
                     /gene="proB"
                     /locus_tag="IVY71_000776"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7984846.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            17365..18612
                     /locus_tag="IVY71_000777"
     CDS             17365..18612
                     /locus_tag="IVY71_000777"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7984847.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            18688..19032
                     /locus_tag="IVY71_000778"
     CDS             18688..19032
                     /locus_tag="IVY71_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984848.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(19149..19475)
                     /locus_tag="IVY71_000779"
     CDS             complement(19149..19475)
                     /locus_tag="IVY71_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984849.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(20090..21292)
                     /locus_tag="IVY71_000780"
     CDS             complement(20090..21292)
                     /locus_tag="IVY71_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7984850.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            21409..21924
                     /locus_tag="IVY71_000781"
     CDS             21409..21924
                     /locus_tag="IVY71_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7984851.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(22123..22569)
                     /locus_tag="IVY71_000782"
     CDS             complement(22123..22569)
                     /locus_tag="IVY71_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7984852.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(22616..24094)
                     /gene="msr"
                     /locus_tag="IVY71_000783"
     CDS             complement(22616..24094)
                     /gene="msr"
                     /locus_tag="IVY71_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7984853.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(24448..25077)
                     /locus_tag="IVY71_000784"
     CDS             complement(24448..25077)
                     /locus_tag="IVY71_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7984854.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(25061..25525)
                     /locus_tag="IVY71_000785"
     CDS             complement(25061..25525)
                     /locus_tag="IVY71_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7984855.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(26012..27280)
                     /locus_tag="IVY71_000786"
     CDS             complement(26012..27280)
                     /locus_tag="IVY71_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7984856.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(27511..28248)
                     /locus_tag="IVY71_000787"
     CDS             complement(27511..28248)
                     /locus_tag="IVY71_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7984857.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(28315..28884)
                     /locus_tag="IVY71_000788"
     CDS             complement(28315..28884)
                     /locus_tag="IVY71_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7984858.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            29012..29884
                     /locus_tag="IVY71_000789"
     CDS             29012..29884
                     /locus_tag="IVY71_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7984859.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         8459 a         6396 c         5405 g         9765 t
ORIGIN      
        1 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
       61 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
      121 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
      181 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
      241 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
      301 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
      361 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
      421 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
      481 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
      541 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
      601 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
      661 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
      721 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
      781 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
      841 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
      901 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
      961 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
     1021 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
     1081 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
     1141 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
     1201 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
     1261 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
     1321 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
     1381 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
     1441 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
     1501 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
     1561 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
     1621 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
     1681 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
     1741 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
     1801 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
     1861 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
     1921 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
     1981 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
     2041 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
     2101 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
     2161 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
     2221 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
     2281 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
     2341 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
     2401 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
     2461 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
     2521 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
     2581 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
     2641 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
     2701 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
     2761 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
     2821 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
     2881 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
     2941 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
     3001 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
     3061 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
     3121 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
     3181 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
     3241 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
     3301 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
     3361 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
     3421 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
     3481 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
     3541 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
     3601 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
     3661 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
     3721 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
     3781 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
     3841 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
     3901 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
     3961 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
     4021 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
     4081 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
     4141 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
     4201 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
     4261 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
     4321 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
     4381 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
     4441 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
     4501 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
     4561 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
     4621 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
     4681 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
     4741 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
     4801 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
     4861 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
     4921 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
     4981 gctctgcaag ttgcgcactt aactttgtct gatccgaata attcaaacta tttgtttcca
     5041 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
     5101 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
     5161 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
     5221 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
     5281 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
     5341 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
     5401 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
     5461 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
     5521 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
     5581 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
     5641 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
     5701 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
     5761 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
     5821 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
     5881 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
     5941 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
     6001 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
     6061 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
     6121 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
     6181 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
     6241 tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct tgataaggac
     6301 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
     6361 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
     6421 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
     6481 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
     6541 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
     6601 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
     6661 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
     6721 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
     6781 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
     6841 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
     6901 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
     6961 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
     7021 gcaattttcg attgcttctt ttaataaaag atgctgaatt gctaaaggtt cttgcaatgt
     7081 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
     7141 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
     7201 tataacgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
     7261 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
     7321 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
     7381 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
     7441 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
     7501 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
     7561 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
     7621 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
     7681 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
     7741 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
     7801 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
     7861 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
     7921 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
     7981 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
     8041 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
     8101 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
     8161 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
     8221 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
     8281 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
     8341 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
     8401 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
     8461 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
     8521 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
     8581 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
     8641 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
     8701 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
     8761 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
     8821 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
     8881 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
     8941 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
     9001 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
     9061 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
     9121 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
     9181 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
     9241 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
     9301 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
     9361 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
     9421 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
     9481 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
     9541 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
     9601 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
     9661 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
     9721 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
     9781 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
     9841 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
     9901 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
     9961 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    10021 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    10081 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    10141 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    10201 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    10261 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    10321 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggtt
    10381 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    10441 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    10501 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    10561 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    10621 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    10681 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    10741 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    10801 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    10861 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    10921 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    10981 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    11041 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    11101 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    11161 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    11221 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    11281 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    11341 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    11401 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    11461 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    11521 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    11581 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    11641 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    11701 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    11761 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    11821 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    11881 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    11941 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    12001 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    12061 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    12121 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    12181 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    12241 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    12301 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    12361 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    12421 attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga tattatcgga
    12481 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    12541 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    12601 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    12661 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    12721 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    12781 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    12841 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    12901 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    12961 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    13021 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    13081 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    13141 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    13201 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    13261 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    13321 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    13381 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    13441 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    13501 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    13561 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    13621 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    13681 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    13741 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    13801 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    13861 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    13921 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    13981 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    14041 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    14101 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    14161 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    14221 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    14281 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    14341 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    14401 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    14461 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    14521 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    14581 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    14641 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    14701 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    14761 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    14821 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    14881 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    14941 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    15001 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    15061 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    15121 catttgtttt agcggttttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    15181 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    15241 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    15301 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    15361 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    15421 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    15481 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    15541 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    15601 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    15661 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    15721 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    15781 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    15841 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    15901 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    15961 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    16021 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    16081 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    16141 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    16201 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    16261 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    16321 tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc
    16381 tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta
    16441 attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt
    16501 actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca
    16561 catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt
    16621 gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc
    16681 tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg
    16741 tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc
    16801 ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca
    16861 acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa
    16921 tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa
    16981 cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct
    17041 gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga
    17101 cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga
    17161 taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg
    17221 cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat
    17281 tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg
    17341 tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa
    17401 gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa
    17461 atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta
    17521 gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct
    17581 gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt
    17641 ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg
    17701 ccactaggcg tgatcggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct
    17761 agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc
    17821 tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct
    17881 tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg
    17941 acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa
    18001 gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat
    18061 aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca
    18121 tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg
    18181 ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg
    18241 ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta
    18301 gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat
    18361 cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt
    18421 ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac
    18481 ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt
    18541 ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa
    18601 atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag
    18661 gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac
    18721 cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa
    18781 ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc
    18841 gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta
    18901 gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg
    18961 attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg
    19021 actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact
    19081 gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg
    19141 gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg
    19201 tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt
    19261 tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca
    19321 gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct
    19381 ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt
    19441 gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt
    19501 aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg
    19561 gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa
    19621 ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc
    19681 cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaatcagc
    19741 tgaccctcgg tattagctca aaaaacggta aaatatgaga agctattttt ccgttttgtt
    19801 tctctaatac ttaggataaa acgctgattt tacaacctct gttaaacggt gttcaatcag
    19861 ctgtccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt tccgtttgtt
    19921 tctctaatac tcaggacaaa acgctgattt tacaacctcc tgataaacgg tgttcaaaag
    19981 cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagaagctat ttttgtttgg
    20041 ctgttcttat gacttgatgc aggacagctt tttcacaacc tctgatttct tattcttctt
    20101 catttgtagc aaaataatta cggtgataaa tgatacaaca tacactgata agaacaatgg
    20161 cacctgcaaa ataaaacgca tagctttcac caatatgttc accaatcaca cctgcaataa
    20221 atggtgctaa agctccaccg aaaaagcgga caaaactata gctggacgaa gcaacgtttc
    20281 gttgcagacc tggtatttcc atagcaatcg tcgtcaacag tgtattgata attccttgga
    20341 aaaatcctgc taacacaatg ctgatcggta caaggactgg catagaagtt ccagctccta
    20401 ataacagcaa acaaataatg aataagacaa atgctgctaa aattgtccga taagtcgtaa
    20461 acttccgctc aagagccggt gcaacgaaaa tcgaagcaat agccagaagc acaccccagc
    20521 caaaaaatac aaaaccgact tctaattcag aatagccaat caataaaaat ggcgcatacg
    20581 caagtaaagt aaagaatccg aaattatata acaatgcgat tagtccaact gaacgaagtt
    20641 tatgattgct taacgctttg acaccttccc aaaggcctac ttttctcttt ggtttctcag
    20701 tttcaggtaa aagtaccgct agggctataa aaccgcaaaa catcaaaaat gctaccccaa
    20761 aaaatggata acgccatgaa ccactgccca agaccccacc gactagaggg ccaacagcca
    20821 ttccgcagcc aagtgctgct tcatacatca tgattgcttt ttctgtgtta ccaactaaaa
    20881 tactgacgat cgttgctaaa gcagtagaga caaataaagc attcccgaca ccccagccgg
    20941 cacgtaaccc aatcagcatt ccgatattcc cagataatcc gccaagacca gcgaatacaa
    21001 cgatgataaa caatcctgtc atcaatgttt ttttcaaacc gatccgacta gataagaaac
    21061 ctgtaaatag catcaccaca ccagtgacta acatgtaact agtaaataac agggtcgttt
    21121 cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa gattgggtct actagaccca
    21181 ctcccataaa agcgatcata caggtagctg ctacaactag cgctgaactt ttcccctttg
    21241 tttcttcatt tgtttgttct tgtatatcta tttgattctc caatatttcc atccctttct
    21301 ctctattagc atcatacatg atttatttta catgtcaata tgcacatgta aaacgtggaa
    21361 actttactgt gatataataa agaaaataag acgagaggta ggatttttat gaatacttta
    21421 gcctatatat tattatgcgt gcttttatcc aaaccacgtt ctgggtatga attgaagcag
    21481 ctgatcacaa ttttttggga agcccatcac agtcagatct atacaacgct tgccaagtta
    21541 gaccagcagg gttatgtgac cgttttggac aaagacgagc actctcaaaa aaagatctac
    21601 cacttgactg atgaaggacg tgctcttgta gaggaatgga tcaaagagga aactcccgct
    21661 cctacgcaaa aagatgaatt tctagcaaaa atctatgctt tttcgacgct cgataaaaca
    21721 acagctactg gactgctatt cactagagaa caacagctga acaaaatcat gcaaaaaaat
    21781 caaaaaaaat tgaatgacct ctcgctagac aagaaacaag aatttggacg ttatgttgtg
    21841 atccagcgca gaattatgct ttgtcagcag gaactccaat ggtgtcagca agtaagagaa
    21901 caaatgcagc agttttttga gtaagggata aacagaacct ttctagaaat atctattgaa
    21961 gagcaaatta tcttcaataa aagagcaata cagttggaaa agaaaaagcg atccgaacga
    22021 caggccaaaa cctatcgttc ggatcactat ttttctagaa ctgcattcaa gagactatga
    22081 caaaaagcat gccaaggcct tttttcaaat aacagtgttc catcattcat tactttgaat
    22141 tgcttttagc acacttcttg aaagcgggcc aacgatgatc aactgcaacg gtaaagcggc
    22201 aatcacattc attttccaag cattcatata agcagagaat aattgatcag gtattccttg
    22261 ttccatgatc acaccgaata aagacataca tgtcaccatt cctaaaacca tcaagcatga
    22321 aatagttaag atcatttgga ccttgctttc tggatttact ggtagtttaa aggcaatttt
    22381 tttcgccagc acaccgatga tgaagacatc caaaatgaaa gccaccacga atccaggcac
    22441 tagaccaatc aacaaacttg ataaatgaag atcttgatgc agccataaat tatacgcgct
    22501 catccccaag accatcaaaa aacacatcat tgttgtaaaa attattcctt cttttttatt
    22561 tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa atcatcgtag cacatttaaa
    22621 aatttctcgt aagtactttt tttattttct gcccttttat ttcataaact tcatcagccg
    22681 tacgctcgac aaagtaagaa tcatgagaag taaacaaaat cgttcccgga tatatttgca
    22741 ttagcttctc taaagcttcg atcgttgcca tatcaataaa attagtcggt tcatctaaca
    22801 gcaacacatt acttggctta gtaaaaagca aagcaatcgt cagacgggtc gcttctcccc
    22861 cacttaatgt acataacggt ttcgtcaaag tttcctgagc aaaccctaag tgattcagca
    22921 aactacggac aagcgattct gaaaactctg tttgtctcat gacaaaacga atgatggatt
    22981 cttcagatgt catttgataa cccttttgct gatatacttg aaagcttact ttcggagaga
    23041 gaaggattcc ttctccttgt ttgcggatat gttctaataa ggttgtctta cctgaaccat
    23101 tttcaccgac aatcgcgatt tttttaccta aagaaaactg aaaatcacca tttaccagta
    23161 atgttctccc acttctttcc aattggacat tttgtcccat gatgggatag cggctgtgga
    23221 gttcaagaga ttttggtact ggaaaacgga tttgtttcat ctgctgcggc ttggttgttt
    23281 ctcctatttt ttggagtctt ctctctaatg ttttcgcatt cttttggatg gctttttgta
    23341 cactgtcttt ttgtttagag gaagacaacc gatccagtct gatttgttgc tttttctttt
    23401 tactggacac tttttccaac ttttccgctt gttttttctt ttcttggatc gatttttcca
    23461 atcgtttttt ctctttcatg atcgactctg cttctcgtag ctgagtcttt ttctccaatt
    23521 ccttttgagt gagataggca ctataattcc ccgaaaactc tcggatttct ccgtctttta
    23581 cttcccagat tttcgatgct agctcatcta gaaaatagcg atcatgacta acaaaaacca
    23641 gcgtaccata ataataacga agctcttcaa tcagtcgatc aatacttttt ctatctagat
    23701 gggttgttgg ctcgtctaga agcatccctt gttcataaga agaaagaaga cgtatcaact
    23761 cgatctttcg ttcttcccct ccgctccatt tccgctctga agtttcttcc agtcttaact
    23821 ggaaatgact ggctaattcc aaatttaggt cctttgcttc agcgagatag gttaattgag
    23881 gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg atcgggaaaa agttcaccgg
    23941 ctattagttt gagcaaagtc gatttaccga caccattttc tcctatgatc cctatacgat
    24001 cttgctgata gacacgaaga gaatcaatag atagttctag ctggttgcca aagctgactt
    24061 gtagatttgt tatatttact gctaaatttt ccatatgttt tacactccta tttcatgtaa
    24121 aacaacaatt atcgatacta aaaaagagca gaaaaatctg tttgcaaaaa aactgcatca
    24181 catcttttcc tactctgaac gaaataacta tgactaaatc accaaatctg tcatagcttt
    24241 cacctattct actgtcgttc gaaaaaatgg acatactcca tccatgaaat tgttgtttat
    24301 acatgtcttt tgaatttatt tagttgttat tcaaaagttc gaaacgtaat ttcatcgatg
    24361 cagtcatttt tccgacctcc ttttttatta ggttaccaca cacgattcaa aaaaagcaat
    24421 agctactgaa tagtaactga aaacctttta ttcgaacata gtgatctttt ctggtgctaa
    24481 acgaacagac tcgtaacctt cttctaccgc ttttcctatt gataaaataa taactggaac
    24541 ataacggtct ttatccaaac caaatgcctc tgccaactga tctgcttcga atccaccaat
    24601 tggatttgtt tcatagccat gatcacgcgc aactaacatg aattgcatag ccgctaagct
    24661 agaatcgact ttgactacat cgttcatttc ttctcttgaa aaactcttgt aataaggaat
    24721 gatcgcagct aattgttgat ctctgacttc ttttggcatc ttccctgatt cgtaagcttg
    24781 gttgtagatt tcttcgccat attcgtaaca ttgcatatcg ccaaaaatca tgaccattgc
    24841 agaagaagta tcattttgtc tcgtattgaa acggatcaaa ggacgcaaga tttcttttgc
    24901 ttcctcgctt tcagcgacca caaagcgcca tggtttcata ttgacagaag aaggtgcagt
    24961 cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg attttgtatg tttcatcata
    25021 tacgcgaacg gatcttctac cagtcataat tgttgaaaaa tcattttttt gttccattat
    25081 tattaacctc tttcgtttct gttgttatat tgcaaagttg ggtattcaat ttatctaaaa
    25141 gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg ggatagcacg gaacctgtat
    25201 ctttatggtc catttcacaa tgcttgattt tcttctgggc tttttctgtt agcgctacat
    25261 acacttcccg gttgttctga ggatttctct gacgcgaaac atagccattt gcttccaata
    25321 ccttcagatg acgagtaact gctccttgat caatctgcaa cttttcttgc aaactgcttt
    25381 gtagcaatgg attttcttct ttcagtctca tcatgatctc gtatctagta agacttatac
    25441 cgatattttc ttcaaagatc tggacgattt tttgatcggc taatttgatt tgatataaaa
    25501 ggtgactaat ctcttctaaa gacataattt cctcctttct aataattgac tcgtcaacga
    25561 ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa agaatcagct gtgtaataaa
    25621 aaaaacgctc tcaatagaaa agtatagaga aaagaaattt tttgtagaat gaacggaaaa
    25681 aatgataaag aaagcacaac gctaaaacca agattattga ttcttatcaa actatagaag
    25741 atagctcttc tgcagtagat aattttacac cgccaaaaat ttccgaaatc gccttttgca
    25801 agaatcccgt cgaatattag aaattggatc agtatgctta aacagtaata aaaaagttat
    25861 gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt caaaaacttg catctgcggt
    25921 aataagttca accaaacaaa aaacatgaaa atctgttttt gtttggctgt tcttactact
    25981 tgatgcaggg cagctttttc actacctctt tttaataacc ataaaatcct attccttcat
    26041 atctccagcc catcggtgtt agccaatctc tttccttagc actcaaggta tagtgatggg
    26101 taccagtttt ttcatttgga ttaaatagtc gataaattgg cttggaacca ccagaataaa
    26161 aacttagacc ttcataacgc caaccgattt ttgttaaaga gttgatctca gattgtgcca
    26221 ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg gtagacaggt tgtccgctgt
    26281 ttggtgcttg ccaagcaatc ccttcatatt cccagcctac agttggcaga taattttttt
    26341 ctccggcatt caatgtatat aaatgttctc ctgtatgacg gttatacaga cgatacatcg
    26401 gagaagttga agttggtgct gtatcatact gtgtcagatt ataggtttcg attacattgt
    26461 tcaatgtaga accataatta ggcgcagtgg cataacgacc tgttagccaa gccgtagcat
    26521 ctttgtaaga gttcgtattg cttttccatg caccggaata gtagtaaacg cctggctgaa
    26581 aagaagtcgt cttcaatacc gttacgttat cttgcaatga ttctttgtac gaaggatatt
    26641 ttctaaaagc ggcattcacc gtaatccatt gcccattcac atattccgaa gtaggcatcg
    26701 taacagattg gccattatag cttcctttaa tcccaaatag attgtaatta ggggcagaag
    26761 ccaaagtact gttcccccag ccactttcaa cgatggcttg agcaatcatg actgatgcat
    26821 ataagtcatt ggcacttgcc aaactttgtg cagttgaggc aatactatca atgaacgctt
    26881 gttgagaatt agcagatgag cgagcataaa aagcatagcc agtatagttt tcgaattcag
    26941 acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc tgtcaattgg ttcgtgattt
    27001 cttctgcatt ctctggattg ttctgagctt ctgctgatgt actgtcttcg gtttgtgttt
    27061 gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt attttctgag acagtagctg
    27121 actgcaaagt actttctgta tcgttttcaa ttcctgtttc tgaagaactc gatgtctgag
    27181 ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt gttggcaact gctagatttc
    27241 ctaaaagaat aacgctaaat aataaattct ttgctttcat cttttttctc ctttgaatta
    27301 tgaaactttt cctaaataac agtgtaacta aaaaagtaaa agcaagtcaa tgtccatatt
    27361 taaataacat ttgtttcttt ttaataaatt tgctttttct gcataaaaag acgtaaatga
    27421 agaatattct tctccattca cgccttaaat tgatggtcgt ttgaataact atttaacaag
    27481 taattctctc tggatattat ccgttatgga ttatacttcc tgtgtcgtcg gacggataat
    27541 catgttgctg acagacatcc ggtctggcgt ttctatcgca aatgctacag cagaagctat
    27601 atcttcagat gttaatcccc attctttttg cgcttcatga agttcaagag cagccttttt
    27661 gtcgctgatg gtttgatata attcagtttg aacagctcct ggagagatga tcgtagattt
    27721 gatgttattt tgccgttgct cttgacgtaa tccttccatg attgcacgga cagcaaattt
    27781 tgttccgcaa taaacggcgg aatcaggata aacaacatgt cccgccacag aatcagttga
    27841 aatgatatgc ccagattttt gtttttccat gattggaagt acggcggaaa ttccatttaa
    27901 cacacccatg atgttgatat caagcatatt ttgccattca tcacggtgac cttcaactaa
    27961 tggagcggtt ggcatgattc ctgcattatt gtacaataca tcgattcgac cgaacttttc
    28021 catcgctaaa tcgataactg cttggacttg tgcaaaatct cttacatctg ctgtttgaat
    28081 atatagctca gcttctggta aagattcttt tattgctttt aggcgatctt ctcggcgtgc
    28141 agctataact agtttagccc ctttttctgc taatagcttc gttgttgctt caccgattcc
    28201 gcttgaagcg cccatgatga caatcacttt ttctgataaa tcattcattc tattctcctc
    28261 ctgttttgaa aaatctcttg cttatactca ctatctctta tttcatctta ctgttcaaat
    28321 aatccgttcg acaggtttga cgattttggc agggattcct gcaacgatgg tattgtctgg
    28381 gacatctttc gtaacagttg cattcgctgc aacaatggca ttctcgccaa tcgttacgcc
    28441 tggcagaata gttgctcctg ctccgatcca agcatttttc ttgatgtgga ttggctttac
    28501 tgacaagcct cttcgttctt ttggatcggt tagatggttc accgtgatga ggttgacacg
    28561 aggaccaatc aatactgaat cttctaaaca gatgccacct aagtcaacga acatcacttg
    28621 acgattgata aagacatttt tccctatact aatatgtttg ccgaaatctg tataaaaagg
    28681 cagcagaaca gtcaccgtat catctacttt gtcactaatg atattttcta gccgtttgcg
    28741 gatatctgct ggttcatggt acccagtatt cagcgctgct aaaggctctt cgttttccgc
    28801 ttggatctga tggatcactt caaagagcgg atccgttact cgaatctctt tattgacgat
    28861 ttgtttttcg atgaggcttg tcattttgct ccctcctttt atccttactt tataacggac
    28921 ggcttatgat gtctaatact tataaaacat acttaactat tccttttaac tatacttagt
    28981 ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt cgtatactga cgtatttttt
    29041 agcagtagct agagaaaaaa atatcagccg agcagcaaaa tctctgcata tcacgcaacc
    29101 tactctctca aaacaactga aagatttaga agaagaactt ggtgtgacac ttttcactcg
    29161 aggctcccga gagatacagt tgacggattc tggtcgatat ctttattcaa aaggaaaaga
    29221 aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa tcatctctgg
    29281 agaaatctac attggcggag gcgaaactaa agcaatctct ttcatttctg atagccttca
    29341 ttcgttgatc aaggcgcatc ctgatatcca gtttcattta tatagcggga atgcagatga
    29401 tatcatagac aaactggata gcggattact tgattttgga ctcgtaatcg aaccgacaaa
    29461 caaacaaaaa taccagtcat ttcgactgcc agcccaagat cgttggggac ttttgataca
    29521 tcggaatcat cctttagcaa agcaaacagt catccatccg caggaactga aaactgttcc
    29581 tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc taggtttcag
    29641 catagatcag ttatcgatcg tcggaacata taacttattg tacaatgctt ctttgatggt
    29701 cgataacggg acttttggtg cattagctct agatgggatc atcaatactt atgggacgga
    29761 aaaagtattc atcccactcg atccgccatt acaagcttca ctgaatctca tttggaaaaa
    29821 agaccagcct ctttctcctg ctgctcgtaa atttttagat gtcctaacgg agaaatacag
    29881 ttaagtagag tttttttcct tcgctttcag ctttttaaca acgaataagt taagcaaaag
    29941 atccaatgat taaataacat aagtttctta atttgttcct tccttttatt tctgataatt
    30001 ccagtaaatt actataatta taaaa
//
LOCUS       DACCXW010000022        29727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCXW010000022 DACCXW010000000
VERSION     DACCXW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.022"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1223)
                     /locus_tag="IVY71_000790"
     CDS             complement(<1..1223)
                     /locus_tag="IVY71_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP7984860.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIK"
     gene            complement(1223..2005)
                     /locus_tag="IVY71_000791"
     CDS             complement(1223..2005)
                     /locus_tag="IVY71_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984861.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            complement(2022..3779)
                     /locus_tag="IVY71_000792"
     CDS             complement(2022..3779)
                     /locus_tag="IVY71_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP7984862.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            complement(3754..6585)
                     /locus_tag="IVY71_000793"
     CDS             complement(3754..6585)
                     /locus_tag="IVY71_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7984863.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            complement(6582..7349)
                     /locus_tag="IVY71_000794"
     CDS             complement(6582..7349)
                     /locus_tag="IVY71_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7984864.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            complement(7382..10501)
                     /locus_tag="IVY71_000795"
     CDS             complement(7382..10501)
                     /locus_tag="IVY71_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7984865.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            complement(10518..10805)
                     /locus_tag="IVY71_000796"
     CDS             complement(10518..10805)
                     /locus_tag="IVY71_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984866.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(10859..11227)
                     /locus_tag="IVY71_000797"
     CDS             complement(10859..11227)
                     /locus_tag="IVY71_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984867.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            complement(11273..11785)
                     /locus_tag="IVY71_000798"
     CDS             complement(11273..11785)
                     /locus_tag="IVY71_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAP7984868.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(11796..12188)
                     /locus_tag="IVY71_000799"
     CDS             complement(11796..12188)
                     /locus_tag="IVY71_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAP7984869.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(12188..12526)
                     /locus_tag="IVY71_000800"
     CDS             complement(12188..12526)
                     /locus_tag="IVY71_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP7984870.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            complement(12510..12836)
                     /locus_tag="IVY71_000801"
     CDS             complement(12510..12836)
                     /locus_tag="IVY71_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984871.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            complement(12833..13162)
                     /locus_tag="IVY71_000802"
     CDS             complement(12833..13162)
                     /locus_tag="IVY71_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAP7984872.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            complement(13174..13437)
                     /locus_tag="IVY71_000803"
     CDS             complement(13174..13437)
                     /locus_tag="IVY71_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984873.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            complement(13455..14378)
                     /locus_tag="IVY71_000804"
     CDS             complement(13455..14378)
                     /locus_tag="IVY71_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7984874.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            complement(14391..14993)
                     /locus_tag="IVY71_000805"
     CDS             complement(14391..14993)
                     /locus_tag="IVY71_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP7984875.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            complement(15120..16046)
                     /locus_tag="IVY71_000806"
     CDS             complement(15120..16046)
                     /locus_tag="IVY71_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP7984876.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            complement(16051..17550)
                     /locus_tag="IVY71_000807"
     CDS             complement(16051..17550)
                     /locus_tag="IVY71_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7984877.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            complement(17563..18807)
                     /locus_tag="IVY71_000808"
     CDS             complement(17563..18807)
                     /locus_tag="IVY71_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAP7984878.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            complement(18794..19327)
                     /locus_tag="IVY71_000809"
     CDS             complement(18794..19327)
                     /locus_tag="IVY71_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAP7984879.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            complement(19354..19578)
                     /locus_tag="IVY71_000810"
     CDS             complement(19354..19578)
                     /locus_tag="IVY71_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984880.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            complement(19585..19797)
                     /locus_tag="IVY71_000811"
     CDS             complement(19585..19797)
                     /locus_tag="IVY71_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984881.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            20159..20338
                     /locus_tag="IVY71_000812"
     CDS             20159..20338
                     /locus_tag="IVY71_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP7984882.1"
                     /translation="MYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDVTK"
     gene            complement(20331..20672)
                     /locus_tag="IVY71_000813"
     CDS             complement(20331..20672)
                     /locus_tag="IVY71_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984883.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(20971..21438)
                     /locus_tag="IVY71_000814"
     CDS             complement(20971..21438)
                     /locus_tag="IVY71_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP7984884.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(22030..22317)
                     /locus_tag="IVY71_000815"
     CDS             complement(22030..22317)
                     /locus_tag="IVY71_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984885.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(22314..22523)
                     /locus_tag="IVY71_000816"
     CDS             complement(22314..22523)
                     /locus_tag="IVY71_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984886.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            complement(22520..22717)
                     /locus_tag="IVY71_000817"
     CDS             complement(22520..22717)
                     /locus_tag="IVY71_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984887.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            complement(22714..23040)
                     /locus_tag="IVY71_000818"
     CDS             complement(22714..23040)
                     /locus_tag="IVY71_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984888.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            complement(23037..23318)
                     /locus_tag="IVY71_000819"
     CDS             complement(23037..23318)
                     /locus_tag="IVY71_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984889.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            complement(23331..23549)
                     /locus_tag="IVY71_000820"
     CDS             complement(23331..23549)
                     /locus_tag="IVY71_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAP7984890.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            complement(23546..23857)
                     /locus_tag="IVY71_000821"
     CDS             complement(23546..23857)
                     /locus_tag="IVY71_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984891.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            complement(24034..24348)
                     /locus_tag="IVY71_000822"
     CDS             complement(24034..24348)
                     /locus_tag="IVY71_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7984892.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            complement(24348..24653)
                     /locus_tag="IVY71_000823"
     CDS             complement(24348..24653)
                     /locus_tag="IVY71_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984893.1"
                     /translation="MSVGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(24650..24811)
                     /locus_tag="IVY71_000824"
     CDS             complement(24650..24811)
                     /locus_tag="IVY71_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7984894.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(24826..25185)
                     /locus_tag="IVY71_000825"
     CDS             complement(24826..25185)
                     /locus_tag="IVY71_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP7984895.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(25182..26033)
                     /locus_tag="IVY71_000826"
     CDS             complement(25182..26033)
                     /locus_tag="IVY71_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7984896.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(26050..26883)
                     /locus_tag="IVY71_000827"
     CDS             complement(26050..26883)
                     /locus_tag="IVY71_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAP7984897.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(26890..27576)
                     /locus_tag="IVY71_000828"
     CDS             complement(26890..27576)
                     /locus_tag="IVY71_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984898.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(27582..28328)
                     /locus_tag="IVY71_000829"
     CDS             complement(27582..28328)
                     /locus_tag="IVY71_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7984899.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(28321..28662)
                     /locus_tag="IVY71_000830"
     CDS             complement(28321..28662)
                     /locus_tag="IVY71_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984900.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(28841..29548)
                     /locus_tag="IVY71_000831"
     CDS             complement(28841..29548)
                     /locus_tag="IVY71_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7984901.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
BASE COUNT         7987 a         6410 c         4705 g        10625 t
ORIGIN      
        1 attttgatgt cgtacaactt aaatccgcca tttattttat ccctgtcaac aaagctaata
       61 ctcatataaa atcgatctaa atttgtgatt tgataattaa cgttagctgt acctttgatt
      121 gttatttctt tccctacatc atctgttgta atatttgtac tatttgcttc caataatatc
      181 ttttctcctg gtgatgtacg atacaccaaa cgtaatttat caatagctcc tgtagttcct
      241 tcatcaaatc gaactttcgc actcagagta taggttttcc cactagcaag ctgaggtgta
      301 ttaatacgcg taaacataat aatgcttcct gtaccatcag aagtaaaatg tagcttttcg
      361 ttatccgaag ttagtgaccc gtggtaacca acgttaaaat cgctcgattt tagtttggac
      421 attaaattcg gattcccact ataatcataa ttcccgaagt cgatgctgtt actgtacatc
      481 tttttcagct tgccgaggtc gccgatttgc tgattggttt gattcatctt gtcttcaact
      541 gctttaagat tgttttgtgc cgatgtaacg ttttgagaaa cggtatcaat cttagtttga
      601 gtagcttgta gttgctctgt tacctcgtca acagtttgat caattgcttg tgtggctccc
      661 tctacaaatt catttatttg ttggtttgcc ttttcaatcg tagaatccac atttgttaat
      721 gctgcgttaa cagcatctgt tgcttcttgt ttgactccat caagtaattg ttgaaagtct
      781 gcaataaagt agtctgcttt atcttgcgct agtccgtcga ttgctgaacg cttcatacgg
      841 aaggtgaagc ctaagttatc acttgttgat ccgtctggat actcgatgta aacataagct
      901 tccaccatac cgttataagc tttcatcgct tctggcaaga tatatacgac ctgtccattc
      961 aagaagcttt cagtgatgag gtttttcgtg ataaaaggga ttggctcttt cgtgatcatt
     1021 ccatctactt catcatagat aaacatcaac aagcgcaagt tagctcctag caagtccgcc
     1081 ggtgtgccgt tctgttgttc gacgttgaac tctaacgcta tttgattatc gtatgattta
     1141 aatacaagcc ccgttgcttg caaatcgtag tctttcggct gtgtaggtac tttgatagag
     1201 ccttttttga tgacatgcgc cattatttca caccttcaat cttagttatt ttcacgttgt
     1261 tgctcattgc gggattggaa acattgccgg atgaaatatc taaagctctg ccgtttgaca
     1321 tcgttatctt tcgcgcttcg attgtcagct caaattcgat gaacgtcact cccgcgtctc
     1381 cattccataa attcgctttt gtaattcgcg cgtatctttg acgtttcact tcttctacaa
     1441 agtctccgtc accgtctgtg tagtggatac gaagcgtttg gtaacggaaa gtatcatctg
     1501 gcagattgac tgattgtcct tttcttaatt cgccttcaaa aagaacagaa gtagtgtaaa
     1561 cgcgccgcca gcctaacgag ttataaccct ctaccggttc gctgttatgt gtcattttga
     1621 tgtaaatttc gccgctataa cgagcaaaag cgacgattaa ttttctcgaa tcgtgatccg
     1681 cgaataccat catttcaaca aaactgtcat cttcaatccc gtcagggttg tctgcgcccc
     1741 atccggcttt ggttgcgtat ttgccgggcg gaatgtctaa aatgttcgga tatttttgtg
     1801 gtagcttata atcgcgtaac caacgcccac gcgcttgcat aactgtttcc ggtgtagcaa
     1861 agcccgcgtt gctttcagta gctaggacgt gcgcgtcgtg atccgagccg tcgtaatgga
     1921 tagccgcttg acgcaataac catttcacag cctcttcata actcatttct tcaaaaatat
     1981 ttggtttgct ttgaaaatct aacatttaat tgttcaccgc cttaatagtt ataagtgaag
     2041 tctctcgcgc gccctacgcc tttgcttgtc acttttcttg ttgctgtgtc aacttctatt
     2101 ttataaaacg ctaattcatc cggtgtatca acttggctag ctgtatgaga gaagccgacg
     2161 tcgcaaagaa tcatttttac tgttccaaac gccccttcga cttgttcgtg ccaatgcccg
     2221 cagaaataac ctactacaac gccggcgcct tttgtattca ttgcgaaggt ctttaatccg
     2281 ccgaacgaac cgtcattcgg ttgtcctaat ttagaccaat cgatagttac cggactacca
     2341 gatttaaacc cttctatcaa cgtgctaatc atgttttcat tgcgaacggg aaacttgctg
     2401 agacctaaag gcgtatgtcc tactaacgcc acatgatagt ttctcggaac gttcactaac
     2461 cattctccga aagcgttaat ttgtttggca ctgaaagctc ccggcgctgt atcgctgtaa
     2521 ccgtccacgt acttgtcgcc tgtgccgcct tcataaaaat cacaagtatc gaaacggtaa
     2581 attgctgcgt ttttatctgg gaacaacacg ccaccgtaca aaccgttcca atactcttcg
     2641 aagtcagcgt tgcataacat tccctttcgc ttgcgccacg cggggtcgaa acaagcgtcg
     2701 tgattccctt tgcagataat aaccggtttt tcttgtcctg ctaccgctgc gttagtgaag
     2761 cgtttgagcg tgcctaacat ggaatgtcgc gcgctccatt catcaataat gcctatatcg
     2821 ctgccgagtg aaccaagtcc gccgtcgata ttgtctccgc cgtaaatcat cacatctgtt
     2881 ttgttcccta atttttgaaa ttgagggatt gcgcgccaat gtcttaaata agaagcatct
     2941 ttgtaaccga cgccatctat acgcaagttg tgattatctg cgtgaatgtc tgtaatgaaa
     3001 gaaaagttaa atttgctgtt attaacactg ttgacaaccg tattcaaatt gcgcggcact
     3061 aggtcaacgt atttcatcgt gtcgtaatca aaaaagcgtg ttgtttctct aatttgtgag
     3121 ctgccgactg gcacttggta tttttggttt aaacgatcag ctaatgattc gtagtcgcct
     3181 ttagcttcat tcagaatgtt aatgatcata ccacctggat cgatattttc cagtatttca
     3241 cgattatctt ctaaccactg ctcccagtca tttttgccct gatccatgta atctttgaat
     3301 tttcttagca aatcctcaaa tgtccacaca tagccagagt cacgtaactg gcttctagat
     3361 attccagaaa tgactcgata ggtaaaatcc tgtgtgctaa attgttcact ccaagttcca
     3421 tcaccattaa gtgatcggaa actgaaatgt gcggtgtttt caccacccca ttgccagtca
     3481 ggctcactta aggtgtaaac aagccttgct tgcgctggac tgtattcttg tactttttgt
     3541 tcaacaggtt ggttttcgcc aaattttgtt gtattaataa aaaacggcac taggccctcg
     3601 aatgttttta gtttgccatg ttccaccact tcaacaacga acttttgcgt taaaacatcc
     3661 ccttgccgaa ttcgaaccaa atttattccg ttgtttggtt cggtggtgga taggaccatt
     3721 ttatgctgcg tttctgccat gactatccct cctttagaaa tcgatgtagt ctcttgcatt
     3781 gtggaaatga cctgaagaag atggataaaa ctcatccata aattgaaaat gaagatgttc
     3841 tccagtggat ggtcctgttg tccccatcag tccaatttgc tgaccagcag ttaccttctg
     3901 accttttgag acgtctactc ggctttgatg cgcgtatcct gtatacattc catcagcgtg
     3961 tttaatcact gtccaatttc cataccagtc aaagtaattt gcatcacctg caacaatcac
     4021 ttcgccatcc gctgaagcaa aaataggtgt attaggattt ccatttacaa ggtcaatacc
     4081 gttatgaaat tcttgtgctc ctgtgattgg agaagtgcgc cagccaaatt cgctcgtcac
     4141 tgtgattgga tccgcaatcg gcttcatata tccttttgat acaggaattt ccaaatcttt
     4201 gaacttgtca taccattctt gtgaccatgc cgtccgttct ggatgtggat cacgtggacg
     4261 ttcaaagtta gccacgaatg cttgtgctgc tgtgttgata tcggtcagat tcatgaattg
     4321 tgtccatgtg taaggataag cgctagttgc gatccattga ccatttggtg catgccacat
     4381 caacaattta aactgtgccg tgattgtgtc tggatcatca ctgatgccag cctttgtcat
     4441 taaattgatc atgtagacac gtccgctagt tgcgccagaa gaatcggtcc attgccatac
     4501 accataaccg aaccctggtg cgccattgcc ctcatcagcg gttggattag catctgattc
     4561 tccttgtgca tttccaagta aggctgcagc agcttgttgt gtaaaaccgg cacctattgc
     4621 cgttgcccaa atttgccaat atcgtttatc ccgatcagta gttacttctg gtgggtattg
     4681 tccgttccaa ccgttatcgt tccctccgga attgcctcct ccattagtgt caatttttgt
     4741 accgttaaca tagagttctt ttacatctag ccgtccatct atggttatat ttccttctga
     4801 aaaattgccg tcaccataaa gattatattt gcgcttatca gcagtaacat ctgctggaat
     4861 ttgaaaaata ggattccctc gatcgccacc gtccccagca ttaatggaaa aaatatagtt
     4921 tggctctttc cagacagcaa agccgttaat ccctttagaa ccataagtag atacaattga
     4981 gcccaagcgt tctccatgta catcatctag tccagtagac ttgacttgtt tttcaaaaga
     5041 aagttctccc ccctctgcca ctaattggaa atctttatcg tcaaatgttt ttaaagctac
     5101 cccttgcacg aggataccag acagaatgcc tgcgcgaata aaattagcat tgaaagttcc
     5161 atccaacgtc caagcggtcg tgctatcacc attgtgtaca tcttgaattg tttgccactc
     5221 acctttttta cattgtttga aagatatccc tgagttattt tggaccataa aaaagcgtga
     5281 tctaggaatg ttaggtccat ccatataaac agtttcatag atttctctac tatcactaac
     5341 accagcttca attccattta cccaataaat agaaccgcca ttatctcctg cacctcgcat
     5401 aatgtcatct tgatattttc caatctctgt cgattcgtaa aatgtcattt tgctagattc
     5461 taaactatta atattattga caatagaagc cgtttgtttt ctaacatctt gtgttaaatt
     5521 atcccctagt tcgatattcg tttgaccggt aagccgattg aatgtagttt tataaatacg
     5581 agttttatag tgataacctt tatcgtatct gtgaatagtc actgtatttc ctatcacatc
     5641 tcctccagtg acttcagctt tgaattgtac taacggtcta gcagaatcga ttaaggttga
     5701 ataagtattt ttaagtaaat ctgttggatc atctatatca tcaaacacta ctacggtttc
     5761 tcgttttctc attgatccat ctttttgtgg tatcccatac ttttgagttg attccggatc
     5821 ttcaagccaa ttttggcctt taggcttatc taaaggatca ccattcgact ttttccattc
     5881 aacatcagtg aattcaattc ttctaccgta accgtcacca acctcttcgc ctcgcccacg
     5941 acctatcatt gaagttgaga ttgagcttct atctatctct cttacaactg ttaatgcttt
     6001 actaccatat acgaaacgtg tattcgattc ttcgccaatt tgttcatata cttcgatcca
     6061 tttatccttt attccatcag aattcaaaga acacctaaag acaaattcca tacctaaggt
     6121 ttgcaattct ttcaacgctt cttttacaga gacatagtaa aaagttgcag ttactgttgg
     6181 taacattgct tctacgtgac caacgcgcca atttccttca gtaaattcaa tcaatcgatc
     6241 aagaacattt tttaagggct gcccactcgg cctaatatct ttgatgatgt aagcatctaa
     6301 ttcatttgtc gcaaatccta accctgtaaa ctctaatgtt tcaaatgggt cgctaacttt
     6361 agtaattcga tacaacgaaa aagacgactc gttttcacga atcgccatat atcttgcatc
     6421 ctctatttct ttatcatatt ttgtcgtaac gtaaagagta tctttcatta gatcactctt
     6481 atcagaacta atttcttttt cttgggagac ttcaatcaaa cttcttgtat tttttctttt
     6541 aataagtttt tgcaagtgat caaagaaata aactgtctca ctcaaattgt cgccccccta
     6601 tagaatattt taagcttccc attattgcta gttatcttct gaccttgctt aagataaaag
     6661 ttctcaaaat cactttctaa atcaatcata gaagtacaat cttctccgtt tacagttacc
     6721 tgctcatcgg aaaaatcaaa aaccaacacg tctcctgttt ttattgccgc gtcagttatc
     6781 gtgatatttt gttctccgtt tgtaattttg attgaattat tcatggataa agtaacttca
     6841 atttttcttg gtgttatagg aaactgtatc ggatttccaa tatagccatc actaacacac
     6901 tctttcgtat actttagtgg atccgcacag aatacattaa aactcgaaat aatcgagttg
     6961 gagtctcctg gaacagtatc agttgatgta tagcgaccgt agtaataata atctaattca
     7021 tcatgaaacc taatttccac gtcttcattc cggtataaat aattcaacag ttctttgaat
     7081 ttaaactgta gtttttctgg atctctatct tccagcttat atgttatttt tagtgttctt
     7141 gaaggtattt tctgatttgt aatgattgaa ccaatttgta tctcttgctg ttcaacttct
     7201 acagaaagca tttctctacc ttccaccgta agtgtttgat aaccatcaat caaatcttct
     7261 aaatacattc catcgtacat catggcagac gtcggaagga atcgtttaga actagtgaga
     7321 ttaatggttg tatctttgaa tgagtacatt ttattttctc gttgatccaa aatattccct
     7381 cctaaaattc cagattaata tctgcacctt cgcccatagc ttgtgaaata tcgtccataa
     7441 acaatctaaa cgattgtctt ccaagattga ttttaaatac agctggttta gtagagccac
     7501 ccatatttat tttatgttca acttgtgcac caatgttttt atttgcattt ttcaaatttg
     7561 cagctatatc tacatcagaa tttgcattga aaagttccgc gataaagtct gccatacttc
     7621 caacagtatt ctgtacgtca ttgaatcctc ctgtcagacc tttattcaga ccgttcataa
     7681 tagcctgacc agctggaatc aatagctttc tatcgtattg gataggtcct ttgtgttcac
     7741 gaatccaatc accaatacct ccaacaaaat cttgcacaga tttccatgca ttttgtaacc
     7801 cttctagaaa actatccatg atagcttttc cggctgctag taaatcgata tttttcaagt
     7861 tatcaaacca gccagttact ctatcaaccg tgtcactaac agcatttact aaattatccc
     7921 atgcctcttg agcaccattt actaaattgt tgaaagtatc tatagtacct tgttttaggt
     7981 tttcccatcc ctgaatgatg ttatctttgg ttccagtaat taaatcacca atccaagatt
     8041 tgaatgaagt ccaaatgtct ttagcacctt gaactgtatt gttgaataaa tctatcgttc
     8101 cttgctttaa gctgttccaa ctttttataa gagtttgcac gatattatta accgtttgga
     8161 aaaaccattg ttttagatta ttccaaagtt caattgcacc atattttgta tcaatccaag
     8221 tttgaattat ggagaatttt agatttatcc acatttgaat tgcactgtat ttaatatcaa
     8281 tccataaatt agtaaaaaat agtttcaaat caatccaaat catagtcgct tgataaacta
     8341 cttcattcca aatattagaa atactttgta tgaatccagt ccataaacta acggcactat
     8401 ttactattgc accgatatat tccgtaaata tattttttac agatgtccaa atatttacca
     8461 ctgattggac aagtgtgttc cagattagat ctaaatcctc tttcatttgt tcgaagtcac
     8521 cagtaattaa atctataata aacagtagtg gcgcagctat taaagactta atgatttccc
     8581 aagcatttac tattatgttt ttaacctcag caaatatact tgttattgct ttcactatat
     8641 tagagaacac atctgaaaag ctacttacaa atggaccaat atattttagt ataaaatcaa
     8701 aaccagattt gattttactt gtaattgttt tccatgcagt agatatagta tttttaaacc
     8761 cttcccattt ttcaccagca cttgaaacta aatcatcaat aactgatatg acaccttctt
     8821 ttaaactatt ccaaatctct gaagcagagt ttgcaatact tgaccataaa tttgaaaaga
     8881 actcttttgt tccagtccat ttgtttttaa tccaatctgc cgctttccca ggggcttctt
     8941 gaactgtagt ccaaacattg tctgcgcctt ctttaatgga cttccataaa ttgttaaacc
     9001 attctcctgt agatttccat gcattctgaa tccattctac tgccgagctt acagcagact
     9061 tgattccctc ccataagcca atccaaaagt ttctaaaatc ttcactcgta ttccaaagat
     9121 agatgaaacc tacaacgagt agtgctaccg cagctataac cagaaaaacc ggatttgtaa
     9181 gaagacccat tgctttactt aatccactaa acaattttga actttcactt aaagctttaa
     9241 aagctacttt caccctattg atccatataa ttactgaacc aatcataaaa attaacggtc
     9301 ctatagcagc gacaattgct cctatggcca ctactaattt ttgagttgat tctggagcac
     9361 ttacaaattt ttctactaag ccggatatgg catctgctac ttttttgatg gatggtgcta
     9421 gaatcttttg aattacaata gctgctgact caaaagctcc aaacatttgc tcgatggaag
     9481 aattcatatt atcttgcatg gtccgagcca tatcgtcagc tgcaccatca gaatctttca
     9541 gagattttgt taatttgccc aatgaatcag gtcctttatc aatcaaagcc atcatccctg
     9601 ataatgattc ttgcccatat agtgttacta aagcattttg ttgttgttca ggcgtcaggc
     9661 cttcaaaagc ttttttaagt aattctactt gagtttttaa aggtttcatt ttaccgtcag
     9721 catcataaaa cgaaacacct aaattatcca ttgtatcttg catagccttt gttggccttg
     9781 ctaacctaga caatgctcct cgcaacgttg tacctgcttg agaaccctta atgcctgcgt
     9841 cactcataat accaatagct gctgcagttt cttccaaaga aatccccatt gaattagcta
     9901 caggagcaac atacttcaat gcttctccca tgtctccaac ttcagcattg gtatccgcag
     9961 cagcacgagc aaatacatca gcgacatgtc ctgcttcact tgcttctaaa ccaaatcctc
    10021 tcaaagcagt agcagtattt tcagaagcta gagccacatc ccctccagat acagctgcta
    10081 agtctaaaag acccggcatt gctttcatga tttcttgtgc gctaaatcca gcagaagcta
    10141 agttttccat tccagcagct gattcttttg cgctaaaagc agtttttgct cctagatcaa
    10201 tcgcttgctg tttcatctgt tcgaatgtgt cgccggttgc tcctgatata gcttttacac
    10261 gactcatttg tgcttcgaag tcgccaccaa ctttagcagc tgctacgcct actcctataa
    10321 gaggagtggt aatatacttt gtcattgcgg caccagtacc ttgcatcact ttaccaacag
    10381 cggttgtcat actatttgaa ttcttttcaa aagttttaac agcatcttgt gcatctttaa
    10441 aagtctttac aaatccacta tctgtggctt ttaataaggc ttcaacagaa aattgttcca
    10501 tgattctcct cctttcctca agagttagct ttagttagta agctttggaa ttttttatct
    10561 tgttttgaaa gttcggaaac tcccatgatt gaatcttcga ttttttgata attgaagaat
    10621 tcttcaaagg atcgatatac aggaactgtc tttttgccta cttttttctc cgcttggact
    10681 tgctgatttg cccacgctaa ttcgtgaatc aacttttctt tgtcaagcca agataactgg
    10741 gctgcagtca tacgaatgtt gtattcatat aacgtcattc tttcgatatc tgagatattg
    10801 gtcattccca aatatcgaaa agaattgata agaatttgtt cgtatgccag tgcagaatct
    10861 attccacttg ttgtttttcc gcttctttca atttctgatt caggtttcgg accgctaact
    10921 ttcccgcgtt cgactccgcc aattctttta ggacttcatc aaacaatttt tcaatgtctt
    10981 taacttcatc gatgtaatca tccaattcat ctaatgtaat agattcatct tcagttctat
    11041 ttgctatttc cagaactcgt gataacgtgt taacgttata agaacgtagc tctggtaaga
    11101 cttttgcgga gagtcccatt ccaaattcca tatttccatc gacgaaaggc atcactttat
    11161 ctaattcacg tacaaattta gtgccaaatt taaacgaata ttctttacct ttaattttta
    11221 atttcaatgt ttttcatcct cctaaaataa aaaagagagc atctaagccc tcttatgctc
    11281 ctgtcgaagt tgctttcacg gtatctttga atgcatattg aacaacatcg gcttgatctt
    11341 ctgtcaaggt tgcataacca tcttgaccaa caccatttac tgcaaatgat aaacttaatt
    11401 caacgttatc ctctgcagca gccgatggag taaattcaga cacatatgct tggtaataag
    11461 tagctttgta cttatttgca ttatcatctg ttccctgttc tgctttatta atttcccaaa
    11521 tttcaatgat atccccattt aataaagctt gtttcatttc atctacatga gaatctcctt
    11581 tagcaactat tgaagtagcc gaaaaatcat attcaaccgg gcttaaactt tgaacgtttc
    11641 catcttttgt cactgtagag tctgaatctc ttgataaacc attttcgtgt tctgtttgaa
    11701 atgccatttt ccaagcggct tcctgagttt cttttttcaa taagcgataa agcaaaatga
    11761 catcaatacc ttttaatgct tccatgttct tcctcctatc taattctaaa ttcaagtgtg
    11821 acaaccgccc gttttagcgg tgtattcgtt gttgtgtcgt ccatcacttg aattccactt
    11881 gcttgataat ttaaagccca ataatagcct tctgtggctt ctatcaatct agcttcatta
    11941 aaaagagcag atgccatatc tgacaactgc tttcgtttct tctgtaatcc ccaaacggat
    12001 aaaaccacaa tcacagaccc tttaatgtca gttttattta cttcatggat tgtctgagtg
    12061 ttctcaaatt ccacaaaagg ataaccaaca tcctctaaag ttttgtaatc gtatgtttta
    12121 tatccaagtt tgttttggga tattttaaaa agttcatcaa aaattgattg atctcttgtc
    12181 ttaatcatca tttcaccaag gctttcattt cagccataaa tttgactttt tggtaattaa
    12241 acgctggtct aacataaggc tgtgccgaca taaatcgagt gccatattct acataaggtg
    12301 cataatctgc tgtcggtcct acaataccag ttaaaccagc ttctaaaaga ttcatgttta
    12361 ttgatcttcg taagtaacct gtatccactg gcgcaccttt ttgcattcgt tcagtcattt
    12421 cagcagtatt acttttcacg actttttgaa cgtcattaag cgttgctgct tttttcagat
    12481 gtcgcatcag ctgatcgatt cctttatatt caagttgtgc cttcatcaag aaccacctct
    12541 tgcacaatta aactatttct atatgctgga tttctagctg ttttttgttg ccaagtcttt
    12601 ccttcaatct cgatatagtc aaatgtaggg atagaaaaaa gaggctgcgt cctaatgacc
    12661 ttcgcccctt cttccacact accaaaaata gtcacacttc tatcagtgcc aatatctgtc
    12721 acgtttgcct ctgttcttgt tctttctggt ttgccttcaa cccactcacc gagatctgga
    12781 tcatatttag agtcagatga acgtttaaca aatataattt catctgtaaa tctcatacaa
    12841 atttaaacct ccctcgcttt ggcttgtaca actcttcctg atctttacgc ttaaattcat
    12901 caatctcatt ttgatactct gaaaaatccg agtcaggaaa agccatagat aaaccttctt
    12961 gagaatatga ctgcatacct tcttgaccaa ttctattgaa tcttttcaac gatacttcat
    13021 atacaactgt ttcaaattct ttaggaactt cttgcgtatt taacaaggtt ttcatacgct
    13081 cattcgttct tcgctcaata acttcaagct tttcatctat tgttccttta agaagttttt
    13141 taatatcatc tgcaatcatc atatttttac ttcctaacta gcaggttgac ctgtcacatt
    13201 gattgaagta gtgaattctc cagaagtaaa tgtgaatgtt gctgaccctt ctgctgcaat
    13261 cgttccatca aaaccaccat tttcattttt ggtcactgtt gcgatagctc catcacttga
    13321 agttgctgta gtagctgcaa caacagcagc tgcatcgcta gcatctgcag gcacagctga
    13381 aatagtaaat gttttagtat cgcctacttt accggtccat gtcttttgat ttggcacaat
    13441 accggtagca ggcgttacgc ttttggggaa atcttcccaa atgcttcatc ttttacaatc
    13501 ataaatccaa catccattgt tgcacgcaaa gcaatcagtt cttgctcaaa caagttaact
    13561 ggggttccat cttcattagt taaagtagac aattgggctt cttcagaaat cttaaatgaa
    13621 atattatatg ggatcccata aaacatgtaa ttaaagtctc cagcgtaaag tgttccttta
    13681 tctaaagact taaggtctac tactggtaat ccgtcaattg tattagtaga gcgatcataa
    13741 ataaactcaa catttgaccc gactgtttga gctgcagaac gtaattctgt acgatttttt
    13801 cggtttgaaa taaacgcatt aggttcgaat tcattttctg ctaacttgtc ttctaaggct
    13861 aggatattat cataagtcaa tccgccttca accacattcc ccgcactaat aactgatccg
    13921 tctagtgact gaggaaatgg gttttcttta tttaataagg cagctgcatc aaatttttta
    13981 tagaaagctt cagcaatttt tggctgcatc tcctcaaaga aatctgataa tttataattt
    14041 aaatattcac gagaaaccgg aagaatgaca ccgagttttt ttgcagtcat cgtagcttgc
    14101 atccatttag gtttagacgt tttaattttt tcaccttcac ccacccagta tgcgcctggt
    14161 ccttctgcaa agtattcaaa tttcttttct ttgtcagtca tttcttcgta ttttgctaac
    14221 tgcatgatct tagaattttc cataacttca ctcaaaatga gcgtattata tttatcagga
    14281 atttttccct ctttcgtttc atataccaag acattatctg gatcccatgt ttgagcaaac
    14341 atttgcaagt tcattggtaa aagttgtttc tttttcatta agttttcctc ctatttgata
    14401 attcgatttt tagcagctag tttagccact gtttctttag tatttttcga tgctgtaaat
    14461 tgtccacctt catttggtgg tgtttgtctt gcgttttctc tcttaatcaa agaagcaaag
    14521 ttagtgatga ctgctacagc ttgttttgtg gcatctgcat catcagaaac aatcagacca
    14581 agtaaatcat catcgtgtgg taaatttgca tctgtcagca ttttagaagc ttctttcgtc
    14641 atttcagata gtgcctgtcc acgctttaat tcagcgattt cagcttcttt ttgctccaac
    14701 tcatgctgta gtttttcttc cgcattcatt tttgccagct ttttagcttc ttcttttttt
    14761 gctgctagtt ctttttccca cgctgctttt gctttattcg tctcagcagc gatcattttc
    14821 gctacttcat cacgagaaaa tgttttgcca gtattgtttt cttcttttgc ttcgggcggt
    14881 gtctcttgtg agccagctgg taggtttcct tgttgtccct catcaccaga tccaccatct
    14941 cctggttcag aaaaaaattg taagttcatt ggcataaata aacgtttttt catgattaat
    15001 cctccacggt tacgccgcta cccgataaat ttgaccagtt acgccggtca gccgaaaata
    15061 gctttctctt taacgcctgt aagctgtaag aaggcacaat aaaaagccgt taatttgtgt
    15121 taacggcttg atattcgttc tatataaggt gctgtactgc atcgacaaaa tggatgcata
    15181 ttaggagcgt tacttcctgg ctgcatatcg gcaacatcaa aaacttgatt atttaacggt
    15241 atacatagtt tgcacgccgt tggttctgct atatagatgt actgggtaat gcctgcatct
    15301 ctataacttc gttcttgaat ccctacctga actctagtcg tttcagtcac catcaaacgt
    15361 tgagtgttga acttagtgtt ttctcgtcct tcagctgtta aatattctgc caatttagat
    15421 gcaagttgct tgggattttt ccccatcgtt atacttctga ctaacaacct atctaattct
    15481 gatttcaatt cagattgata catccataag cgatcactaa aagacacgtc atcactcaaa
    15541 aaggagctgt ttattactag ttctatcagc tttgcatatc cacttgaagc aatagtcatt
    15601 tctaaaatac cggcttgtct cttcaattca gctaaaccag cttttgttaa ttcattcgaa
    15661 aagtacttat ccaattcatt aaacagtgaa atcaattcaa gcccgatatt agcttttaag
    15721 agctctaatc tatttacacg catcgtaaga ttgtatagct ttaattcttg gtttgctgta
    15781 ggagaaaaat ctttctcttt gacatacttc tttgctttgc gagcgaatgc tttgacatcc
    15841 atttcactag cacgcttcat cgcttcgcta cgagtgattt tctggccatt ggagaaactg
    15901 tcccactgcg cgtctatttc tttttgtatc gcatcttgtg cgtattgcat gcgactttta
    15961 atttctgcca tgcgcttttt atcatcttta atttgttgct tttgccattc cttttcccgt
    16021 ttgatccaat attcttggga gttcatttaa tcacccccct gtttcatctt ttttgttgtt
    16081 aattacttct tcaccatctg aatcaaaaat gccaatctgc ttttgcgttt ctttatttac
    16141 tcgtttcaac tctgcctgta catctggaac aaaagaagcg agtcctaaga tcgtctcttg
    16201 actgagttca gctccagcat caaccaaaga tttcaactct tccagaatgg ctttaggtag
    16261 attaggcgta aatattacac gtaagccttt caaatcggag ttatccattt cagaaatact
    16321 tgattttagg ctaaataaaa gacgataacg ccgcataagg ccttttttga atagcctttg
    16381 ctttgttgcc gtcatttgtt caaatccaaa taatttatat ttcattgctt ctcccgattg
    16441 cactccggaa aaattgtcat cagtaagatc aggaaccatg gatatttcgt gaatatcctt
    16501 gcgtactctg tatttgtatg cttctacacc gttcacatca tattgtttgt aaatatatcc
    16561 tgcagtcaca cttgttttat taccgttcac atcagttcca gactcaagca aaagcatatt
    16621 cgcttctttc tgcttgatgg cgtcctctgt ggatagtcct gctgcttcaa tatcaccact
    16681 aataactaga agagcatcgt ttagatcagt catataattg gcggtatcag actgccctgc
    16741 atcgtataga tcaatcaaag atagtacatc ttcatacaag cccatccgaa aacgattagg
    16801 agaatactct gtaataggta cctctttata ttcatgcggt tcatcctggg gattctttaa
    16861 ctcaattgct gttagtgtcg tctcatcata agtgatactt ttttcttttg tgtatacgat
    16921 tggttgaatg tactgtttat cagcatcctt gttgaatctt gtcttaggat accgtacagc
    16981 caaaataggc tctcgtttta ctgtagtatc atatacaaca aacgtttcaa atacattagc
    17041 caaatcaaca taatctgtat catctgaatc tcgatagata atctcatagg ctctcccata
    17101 cttatccata tcaagccaga gttcagcatt taacccatct atgtcattat tagtattaaa
    17161 ctcttcgatt tctttttgtt gatttgtgtc ctcgatttgc acttttatag gattgcctgt
    17221 gttgtaccca acatcaaacg tacaaagaac ttttccaaag ttatgtgctg atcggtgatc
    17281 cgctttttct ttttctctgc gcctacggtt atccatgata tttgtatttc tagctttata
    17341 ataatcatct aatacactta gcctttttac ctgatattca tgatgatgtt ttatcattgc
    17401 tgctaaagta tctaaatcgt taagtaaatc ttctgctgag ctaaatctat agtgaagatt
    17461 tgagtctacg ctaaacttta cataatttgt gttcacgtca ttagaataat gtatatcagc
    17521 tccgtattca aactcgttta ccttatccat ttttcacact ccttaaaaca tgcgtttaat
    17581 acgtttaatc ttttcgttga cattaacttt tggtttctta agaagctcat gagtatatat
    17641 cgcatatcga acggagtcta gcacatcatc atattctttt attggctcgc cttttttctt
    17701 gtcccaaatg tattgataaa tttcatcccg aaatttcatc actctatctt gacagataaa
    17761 caatttatct tgtttaaatc tcttggcgac ttcctcaact ccagataacc gtgctttatg
    17821 agcattttta gcattaatct tttctcttac aaacctagcc acatgctccg gtctcgcaga
    17881 gtcacaataa aaaggtactc gtaagccgta acgctcttga atgccttttg ccacatctac
    17941 ccaataatca atctcttcaa attgagtagc atgttcttcg attaaataag ctgttccgtc
    18001 atccgtttca cctataacta cgattgaacc ccagtgttca tatccccagt caacaccaca
    18061 ataaaagttg ctcaaaggtg gtaagtcttt tgactggatg tagtgcttgc tagcatcaaa
    18121 atcacgataa acgacacctt ctgcagaaac ccataaacct ttgatatcac gatcataaaa
    18181 catgccgctt ggtgttgatt ctttaatatt gttacgatat cgttcagata aaaatgtatt
    18241 atcatctaat tcaaagtgga acgattgaat atttttgctt gaattgtcga tatactcttt
    18301 ctttagccag tgttccgggt tgtcagggtt tgtatcagct agtattctcg ctcctgttcc
    18361 tgaacaacgt gaaacgattt cagcaaatac ttcttgtcta gctagcgatg cctcattgat
    18421 ataagcgcca tatgctgtca taccacgaat tgcgccaaca ccgccaatgt ttcctgtata
    18481 agcttgtacg accttaacgc caaatagctt aaagtttcca tgtttatcga atttaggttc
    18541 tatgctgtac atgttgtata gttcctgtaa gatgttcttt tggattgttg cacttgaaac
    18601 tcctgctagg atatacattg gttccttaat gtcttcttca tcggcaatct ttcgcacacg
    18661 tcgcaattca aacaagaaca aatcattgtt tatttttgtt tttccagaac gttttgctcc
    18721 atgtaataaa gtaataaacc aatcattctt aatggtttta tttaacacgt cgatttgctt
    18781 tgggttataa atatcaacta gtgccatcta attcaccact aatcttttct aacaactcat
    18841 caagtttttc ttcggtagaa cgctctgagt ttatctgtaa ttgttctaat tttgcttgca
    18901 ttaattttat ttctgtttga gctttttcta attgcgtttg tgataacagc gatttatact
    18961 tcaaatacaa atccaaggct ttcaatttgc tttctaaatc tggtgtatat tcatattgca
    19021 tatgtttaat aaccttgttt ttctccaagc ggtctatttg cttgctcacg cttatttgta
    19081 cttctccttg ccaaatatct atcaattcat ttaaagcgtc ctcggcactt aatttgcgct
    19141 tcttttcgat aggatttagc cgttcattaa tatactgtat tatgttaggt ttagttaggt
    19201 tttccttacc tattactctt gctgatcgtt tgctataacc tgctttaatg gcggcttgag
    19261 tagcattacc accatttttt atatattcat ccgcaaaagc tttttgtttt ggtgtcaaat
    19321 ttaccacatt caagccacca cctttcaatt ttattaacaa tatctctcaa tatttgtctg
    19381 tatgttctgc tcactaaaat atccatggcc gcagtaacga agattgtact tatcaatctc
    19441 ttttggcgta gcttctctgg tcatttcaat gatggagtac ttctttttaa tctggactga
    19501 ctggacaact ctaattggat catctgcgtt tggttgcgga tatttatttg ataatgatac
    19561 ataccagtag ttcctcatta cgtatcactc tttctgttgg ttactggcag aaaaggttcg
    19621 catcataaat tccatagcct gtgcgtcatt aaatccttgc agaataagct gatcgtagaa
    19681 atacttagct tgttttgcga ttaacgctaa gcttttttga gtttcgtaga atgtcgcctc
    19741 cattgttttg tttgcttcat tcgttttaaa caactctctt aattggtttt cgttcatttt
    19801 tttactcccc ttccaaaata aaaagaccac tcgatgagtg atctaatatg taaaaactac
    19861 acctcataaa cgagatgcag ttatagttct atcaagcaac ctacaccgat tccatcgatt
    19921 actatcgacc tcgccttgct cgtgtacttt gagcgcccat ttccaaccct cagttgccaa
    19981 agtcactggc aaggaatcga accttgcatg gttgccgaag cattgaccta acacacatgc
    20041 ttagcgtcta ccctttccgc cacagtgaca aattaatatt gtgaaaataa atactaagtg
    20101 tataatttta tttatcagcg agtggtccgc tgaaataaat taaaaggtgg tgaaaaattt
    20161 gtactttgtt ataagagaag caactaatgg ccaatattac ttcgttatta agtccgataa
    20221 caatgaagta gtagcaacta gcgaaacata cttgactaaa tattccgctg aacaaaccat
    20281 taattctata aaaaacggaa tcaccaaaga ctctcaagtt atcgatgtga ctaaataaga
    20341 cgactaactt catttgctag ggagttcatt tcagctgctt tttctaccaa agcagttgag
    20401 atactttcta gcttttctga tttaacagaa ttaaatactg gcgtcttgat actaatcgat
    20461 acatcttgac gaggatcagg actcgttgct ctattttttt gcaccactga tctgtacttt
    20521 tctactgaat gatactcaat tacttgttct aaatgttttt tgtactcttt acatacagct
    20581 tctttttgga tcaattctaa acgttgttgc tcaataatat caatcaaccg ttcccgatcc
    20641 ataccttgat actttatttg ctcgacttcc attcgttttc cctccaatac ataaattaat
    20701 aaacagcaac ggatgataga taataagaac aatttagaag gagttggaat tcacatcctt
    20761 attcttaata tttccgttgc tgtctatcga agcttaattg tgaaacaata ataaaacgat
    20821 gttcctttta ttattatttt gtctcagacc tatcactaat ctttcgacac tatcataata
    20881 aatcatttac caggtaagtg attggtataa aaaaggtata aaatggaaac caaatgggta
    20941 atcaaagggt ataaaaagtg taaaaactgg ctacttaaaa gcaaccagtt ctaacgatga
    21001 agcaaattgg atgataattc tgtttgattc tactttaacc gattcttcgc tagtattatt
    21061 cctttgagca gttacataaa tgggcagacc attgatataa cgatcataga atatcttctt
    21121 gcgcctttct gttacatcag gtttatgcgg atgctgaatt gctgaatagc cacgaacaaa
    21181 taatttgtgc aagtactcaa attcttcttg cgcttcctct ctctcaatca tcattcgttc
    21241 tgcttcaaat gtattatttg ctgtagaagg tggaaccaag gagtaagatg ccgttacttt
    21301 aggctcccga ggttgaccta tcctacatct agctgacaga tatgctgaaa gaaaaacacc
    21361 aacattatgt tttgtgcgat ccatatccac atcttttgcc tctggtgtct catacttctt
    21421 tacatcgaaa agtaccatcc attgattcct ccatttatga tataataatt gtgtcagaat
    21481 tattaattaa ggtcggagga atccggcttt ttttataccc atgcttatgc taagcttttc
    21541 aagtagcgag gttgcactcg ttacccatat acatgttgag ctatctggcg gaaaacagat
    21601 ggctcactat ttcaatattc tgctaaggac agccagtggt cggctgtctt ttttatattt
    21661 taatgagaag ccttacttat ttttttatct ttattacgaa aaatgatatt atctattgat
    21721 aataaagcgt ataggagatg gttgagtgac tatgtggagc atgctgttat tttggattcc
    21781 cgtttgtatt ggtatcgtcg cattttgtta ctttgtcaaa cactctagaa caaataagct
    21841 cctcatgtta tcttttttac ctatagtatt ttttattgta caaattgtta aatataccta
    21901 tattgaatcg caagaaatat tcatttttta tgtggtaggt ttatttatct ctgtggtctt
    21961 tttcataatg atactttcct atttttataa aaaataaatt tttctcttag aagtacattt
    22021 tttcgctgtt tatatagcct tcatatccac caatctcgct acagctaaat tctctttgct
    22081 tttcgctaac cacttatcac attccatcgt gttttcaata cgaatgatcg ctgagtgatt
    22141 atagacgtgt tctacatatc cacgaaatgg atagatgaac ccttctgctt cgcagcgaac
    22201 catgtcaccg actttgaatt ttggtttctt acgtttttta ggattctttg tcggcatatc
    22261 tagcattaaa ccgccgatac catgactact agcgtaaaat ccgtctttta atttcatctt
    22321 tctacctcct catgatcgac agtgaccagt tcatatactt gtgttaatcc accaagtcgt
    22381 cgtgaaactt ggttagcttc ttcgaggcta tcaaaccatc tcgcactttg gagtatgtct
    22441 acaatgaata atgtgttagc ccctatagag cgttcatcac ttctataaaa tttattcccg
    22501 aactttacta cataaatctt cattctgctt cctcctcaat cgtccaagca tttgtaaatt
    22561 tctctaagct tattagttca tcttcattta aatatccgta accatataag gattctaaaa
    22621 ggtcttcttt tgaatagcct ctaaaatcaa gtctgagacc gcttaggatg tatacatagg
    22681 ttttatttcc ataaaacttg attgtgtatg ctttcatccc acttcctcct catcgatctc
    22741 tatctcgaca tctctgcctt ctaaaatctg tttggtatca atataggcta cgagtatttt
    22801 tcgattttgg cttaaagcaa tatacggatc aatcaaagca acttctgcgt gtagacgata
    22861 ataagcaata caatctattt ctaaaaatat caaacgatca atagcatcat taatcataaa
    22921 atctgtaacg ttttctgctg tatttaaacc atctgcatat ttaggcagga cttcattatt
    22981 gatgaactca gctacatgtt ttggacataa atcagtcagt tcttgttgtg tatagttcat
    23041 ttgctgtcct cctgattaat atatttcctc aaactacgta tccctttacc acgttttttt
    23101 gttttgcgct caatccacgt tcctgtagtt ccccagtagt caataattcc attatgggta
    23161 ttaattgcaa attgatgatt gtttggataa tgagtaaatt caaatcccaa ggtttcaata
    23221 tttttagttg ctgaattgcc catctttctg acatgttgtt ttctacgttc ttttagatgc
    23281 ggtttaatat ctttccagta ttcgcccata tcgcccattt cgctgtcctc ctatctcctt
    23341 gtccacaata aaaaccacat caactcttct gtcgtaccgt ttaaataaag atttatttct
    23401 gcaccaatcg caactaaaat tccgacaaca aacaaaataa tccctactgc tgtatatttt
    23461 ttgccttttg cactctcgta aatgaggtat gctcctaaca tcgctagtaa gccgccaatt
    23521 acacaccaaa atattgttag gctgatcatt tgctgtcctc caaatcactc gacttcacga
    23581 atacaccatc taccattttt cctgtgcgtc ctttgatttc gttgtatgct tggttcagac
    23641 actcgtacaa atccatatca ttttgcatag ctaaaataat caaggttact actacatctc
    23701 caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca acttccccga
    23761 tctcttcgat cgtttttaac atttgcttgc tggaatcagc ttgatccaat cccttatctt
    23821 tagcccactg ctctactttt gtgattagtt cgtccattat tcattctcct ttatatattt
    23881 aagttggata atacaacttg taacaaatga agctaatgtt aaaattgtgc cgattggtgt
    23941 taaaaaaccg ctttttatcg cattaattaa tatagctaca aatatcgtta tatagaataa
    24001 aaagtgaatt gacgctaacg ttgaattaaa catctagtcc tcctcgaaat actcattcag
    24061 tatctctcta tacttttcta caaatttgaa acgatcctga tgaagtttct tgctccaatt
    24121 tgtttgccga tccagctcac gcatctgatc gaaccctttt tgaatttcgt tgtaataaaa
    24181 ttcaatgttt gctgctgctt tccaatgcct gctacttcgc actcctgctc ctgtttcagc
    24241 catttccaac ttaactaatt ccgctcgttc ttttgctttt ttatctttct gaatcttcat
    24301 catgattttc ttgaggatga tatcactgta ttgtgtaatg agatccatta tttctcctcc
    24361 acatacctaa actgtcgccc ctttgaatca atccataagc tcctagctct atcccagatg
    24421 atgtttttgc tcagtccagt aatttcagat aactgttcag ctgtacccgt tactagaatt
    24481 cgatcaccat gccagattgc aatttttctc ggcgttttcc gtttaggctt ttcagtccac
    24541 attgatttac cgagcttttg gacttctgca actatttctt tgtcttcttg ccaattctca
    24601 gaatgtgtca gttcaatgat tcgtttcatt gctgctttct tacccacgct cattcctcca
    24661 atctacgaat ttcccttctt aaattcacta tgtgcaaatt gattgccttt ctcgccgttt
    24721 cattgaccat cactgccttt atccgttcca gatcgtcaat ctcacgttga aggcttcgaa
    24781 tacgcatttg aatcacttct tctgttgtca tgatggacca cctcgttaaa aacgctcttc
    24841 cttgaacgta ttccgatatt ttttagctaa tatcaacggc acttgatatt gatgacagaa
    24901 caactttgcc ttgatcttaa agtcttttgt ctgcattcct ttgacatcta cgactttgac
    24961 aagtttgccg tttttataaa atgtgaagtc gggaatatac tcgatcttgc gatacttttt
    25021 tccatctagt tcaaatttcg gcatcagctc aaatctttcc tgaagtttta ctttccagcc
    25081 gttcgcttct gcttgccaca aggctaaatc gtaatactct gcttccgcga tagaatcaaa
    25141 cttgatacct cgatgaacag ttttcttatt acggtattta ttcatgcgat actacctttc
    25201 actggtttta tgcgcttgtc tgctgtttgt tggaatttca gcgcataacc ttctgaattc
    25261 ttaaatattc tagaaacaat tctttcgccg taggcttctc ttagttcagg accggataag
    25321 tttgttgtga tgatcgttgc cttgttctgt ctggcttcta agagcgtgtt taacgtgttg
    25381 tttgtaaact gcctactatt tgataccccg ctacctaatt cagctccaat atcgtcaaaa
    25441 accaccaaat cagttgtttt gatatcggct ataagcgatc cttcaatttc ttttctcagt
    25501 tcagcattgt tataagaaaa ctttatttgc tctaataact cttgatagct tataaaaagt
    25561 attttcttgt cataatttga gcgctcaagt atttcccaag ctgtcgccat tgacaagtgg
    25621 ctttttccgc ttcctgattt ccctgataga atgaaatgtg caggatggtt cagtaagaca
    25681 tcatttacaa agcttttagc tctttctaaa gcaattttcg tttcttggtc cactacgtga
    25741 taattctcca ttttgcattt aaacaaagtt ttatctgtta ataccgaacc attttgaaaa
    25801 aaactcaacg ctcgtgcttt taagctgtcg ttatatatcc gttcggtctg tatatcctct
    25861 ttcacacgta acgctttata accacaactc atgcatgttg gtttacaacg ttctgaacca
    25921 tccttatttt tagctcgcca actatacaaa ggttcgctac attctggaca tttcccgctt
    25981 tgcactaata ctcttcttat tagtttctcc atagcatttg ctaggctttc catgtgatgc
    26041 atctcctttt taaattggca agtcgtcata ttcactagga ttgctgtact gtagtttttg
    26101 actttgcttt ttatgattat tcttgtctgc tttgatttcg aatttgagct tctcaaattt
    26161 ttctctcaat ttcttagcac ttctaatatt tccaaaccaa aattcatttg taggtagcca
    26221 attgatcaca tactcaatcg cttctataga cgctttatct ctttcttcca tcaacctgat
    26281 tgtgtctgcc catttttcga tatctacttt gttcatttct tttggaaaat cttcagttaa
    26341 attactttgc aattttttag caaggcgtaa gtgttcgtca gaatacttac ctttcttttc
    26401 ttctttatct atatctatat ctttctctat ctctatctct aactctggtg tagttttgtc
    26461 tggacatttg tccgacactt gtcctccagt tattaaattc cgttttgcct cttctatttt
    26521 ctttctgtat tctctttttc tatctgcttc agttgaggat tttccaatga aactttgtat
    26581 atcagacata taaattgctc cgttatctaa tacgtcaata agctgcaaat cacggaaaat
    26641 ttgtaccgct ttttctacga ctcctacaga atgtcttgta atagttgcga gcattgtaga
    26701 gttaaatgga atcctgtcat taaacatcaa cttaccttcg tgttttagac ttcttaaata
    26761 aagtttgaga agaatattag aataaatata gccatctggc atactttcta agagaaccat
    26821 ctcgtcacta tcgaaaaaat tctcttttag ttttaaatag tagtagcgtt tgttgtcaga
    26881 caatattttt taccctccta ttctaagttt cttaattgtt tcctggttta acttgatccc
    26941 tttgatttga tatttatttt tgaaattgat cacacctata ttgtgtttct ccgtgtgatg
    27001 aatcctgcag agtgctgcaa atgtgtactc tgaatgatca acttctttgc gctttcgtct
    27061 tcctagcgct ttgtcaaagt gatcgatgtc agctcctgtt ttgccacaga tgcagcagac
    27121 tctttttgtg atgcatttgt agaagtaata ttcttgattc gctggtaaaa tctcatagcc
    27181 ttctttgaaa ggaatatgat gttcaaagat gaaatctaag atgatatttg ctaagacatt
    27241 agcatcactc acagttgtat tcgattcgtc tttgaggctt attttgcgcc ctgtgacgcc
    27301 ttcaaaacgg aagtaaaaga attccttcca gaagtccgtt ggcatgcctg tatcgatgaa
    27361 aatatcgcct atcagcgcat agatgaagtt tcgttgctgc acagtaaatc gacgtggatc
    27421 aataaatcga acttcaataa tccgatcgcc atcatatccg tcatacatcg tctttagtcg
    27481 atcaatgttc acttcttcat tgatcgttgc acctatgtct tttcctttga actttttcag
    27541 aaccgctgaa tatgaatcga tcaatggttt aaacactcat atcacttctc ttttgtttct
    27601 tctctgtact gatcttcaat ccagttaacg cctcgtttta gaatgcccaa gtctctcttg
    27661 gtccatttac tgtcatcagc ggttatagaa gccgcatcag tcaatgcaac aattgcttca
    27721 tcaattgatt tttcgtactt gttagcaacc agttgtaaag catccaagaa tagctttttg
    27781 cttctttgag tagctggttc aagcatcgag acatcttctg gcatatcttc gccagcaaat
    27841 atatatagcc ctagcccaaa cattgctaga ttttttacaa gacagcgcat gatcgttttg
    27901 ttgatatcaa acatagttgc tgcttcaact cgcttttcga tttttccaac aatctctttt
    27961 ttcttcgttt cgttattcca ctgataatca ttgacttcgt aggtatatgg ctcatctttc
    28021 attgccttgt ttgcaccatc catgactggt aaccacatgt cacgctttac tccgttgact
    28081 gtgatactgg taaaaaccat atagcctgtt ttcttatcaa agacatacgg cagatatgtt
    28141 tctggatcat gatagatttc ataatctact gcctcacaca ctttgctaac ttctgcccaa
    28201 gcccatgccc acgaaagata agtgaggtta tttcgttttt cagtaacatc tttcacattt
    28261 attttgtata aggtattaaa aagcgtatta tcacttctat ttttcaaagg ttgttccttt
    28321 tcactcatca aattctgcct ccatttcagc aatgtatttc ttacctgatc cgtaataaga
    28381 gatatcaatc aagttatctc tgtcatactc ttctagcgca tcaatcaagc catcttcgat
    28441 gacatagata tattcagttt ttttggactt cctcgataaa tggataagat agacatgatc
    28501 ccaaatactc acaaaatttc ccaaatcgtc ttgatcacat gctagttctt catccgtcaa
    28561 aagattacgt ctgatttttc gattgcttgt ttccttgata ttcgatttgc cccaactagg
    28621 atcagtcaaa tattgatcta gagtggaaag ttcttttttc atgtggtaac atctccttag
    28681 atgtattttc tttgtgactc attgctttgg tcggctgagt cactttttta tttgttgcca
    28741 tgctttttgc ttatcaatat gttgttggct taggatgttt ggtttattgt gtctccacca
    28801 gcgattagca attattacgc ctatttttag cgcttcagct ctattcatcc tctaacaact
    28861 ccatttgctt aataattgtc atcgtcgctg tagaaggcat ccaattgtta ataaaatcga
    28921 taacatcatc gaactgtttt gccttgatac gatttctagc tactgctcca gtgattcgct
    28981 taattccgcc attcaagtct ttaaacaatt ctgcttttgc tttgctattt aaatgctgct
    29041 gattacaaat aactctaatt ttgttgcgaa ccatattaga aattgttcgg taatctggtt
    29101 cagctagaag ttgattgttc ttcaaatcgt ttaaatcaga ctcgatagcg tctactcttt
    29161 cattcgtttc ttcgtttgca gctagagcaa gtaatgccat ttctcgttta gttgctggaa
    29221 ttgatggcaa ttggtctttt aattcttttt ccatcttgtt gaaggcctca atatattgaa
    29281 gtttaaaact taaagctttc ttaccagtga agcccattgc taatagagag aagccgtctc
    29341 tattcatata aataattcgg tatgattgtt tattttgagg atgaacataa gtatcttccc
    29401 aaaataggtc tgcccaattt tcggcaaccc cctcttttag ttcatcaata gccgtcaaaa
    29461 catgtctgtg ctttttatcg aagctttctg caacttgtaa gctcgtagtc acagcttctt
    29521 tatttttcaa aattactaat tcttgcatta tttcttctct cctttttgat ataattctgg
    29581 tggggtgaaa acatgagcag aaaaattgat aatataatat tgttaacaac tattttttat
    29641 ttagtaatcg gagcaactct ttccttactg tcatttttgt ccaacagtag tttgattgtg
    29701 ttaaatcaaa aaagcatatt aacagaa
//
LOCUS       DACCXW010000023        28681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCXW010000023 DACCXW010000000
VERSION     DACCXW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.023"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(353..1150)
                     /locus_tag="IVY71_000832"
     CDS             complement(353..1150)
                     /locus_tag="IVY71_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7984902.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1147..2331)
                     /locus_tag="IVY71_000833"
     CDS             complement(1147..2331)
                     /locus_tag="IVY71_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7984903.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2356..3603)
                     /locus_tag="IVY71_000834"
     CDS             complement(2356..3603)
                     /locus_tag="IVY71_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7984904.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3818..5563
                     /gene="dnaX"
                     /locus_tag="IVY71_000835"
     CDS             3818..5563
                     /gene="dnaX"
                     /locus_tag="IVY71_000835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7984905.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5583..5897
                     /locus_tag="IVY71_000836"
     CDS             5583..5897
                     /locus_tag="IVY71_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7984906.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5922..6518
                     /gene="recR"
                     /locus_tag="IVY71_000837"
     CDS             5922..6518
                     /gene="recR"
                     /locus_tag="IVY71_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7984907.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6582..7226
                     /locus_tag="IVY71_000838"
     CDS             6582..7226
                     /locus_tag="IVY71_000838"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7984908.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7241..7570
                     /locus_tag="IVY71_000839"
     CDS             7241..7570
                     /locus_tag="IVY71_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984909.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7584..8522
                     /gene="holB"
                     /locus_tag="IVY71_000840"
     CDS             7584..8522
                     /gene="holB"
                     /locus_tag="IVY71_000840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7984910.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8558..9382
                     /locus_tag="IVY71_000841"
     CDS             8558..9382
                     /locus_tag="IVY71_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7984911.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9375..9722
                     /locus_tag="IVY71_000842"
     CDS             9375..9722
                     /locus_tag="IVY71_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7984912.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9791..10663
                     /gene="rsmI"
                     /locus_tag="IVY71_000843"
     CDS             9791..10663
                     /gene="rsmI"
                     /locus_tag="IVY71_000843"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7984913.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10772..11893
                     /gene="dinB"
                     /locus_tag="IVY71_000844"
     CDS             10772..11893
                     /gene="dinB"
                     /locus_tag="IVY71_000844"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7984914.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11947..12549)
                     /gene="nrdG"
                     /locus_tag="IVY71_000845"
     CDS             complement(11947..12549)
                     /gene="nrdG"
                     /locus_tag="IVY71_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7984915.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12864..15053)
                     /gene="nrdD"
                     /locus_tag="IVY71_000846"
     CDS             complement(12864..15053)
                     /gene="nrdD"
                     /locus_tag="IVY71_000846"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7984916.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15379..16146
                     /locus_tag="IVY71_000847"
     CDS             15379..16146
                     /locus_tag="IVY71_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984917.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16136..18178
                     /locus_tag="IVY71_000848"
     CDS             16136..18178
                     /locus_tag="IVY71_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984918.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18459..18608
                     /locus_tag="IVY71_000849"
     CDS             18459..18608
                     /locus_tag="IVY71_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7984919.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18619..20133
                     /gene="dltA"
                     /locus_tag="IVY71_000850"
     CDS             18619..20133
                     /gene="dltA"
                     /locus_tag="IVY71_000850"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7984920.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20130..21335
                     /gene="dltB"
                     /locus_tag="IVY71_000851"
     CDS             20130..21335
                     /gene="dltB"
                     /locus_tag="IVY71_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7984921.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21380..21613
                     /gene="dltC"
                     /locus_tag="IVY71_000852"
     CDS             21380..21613
                     /gene="dltC"
                     /locus_tag="IVY71_000852"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7984922.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            21616..22884
                     /gene="dltD"
                     /locus_tag="IVY71_000853"
     CDS             21616..22884
                     /gene="dltD"
                     /locus_tag="IVY71_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7984923.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            23025..24107
                     /locus_tag="IVY71_000854"
     CDS             23025..24107
                     /locus_tag="IVY71_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7984924.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24107..24910
                     /locus_tag="IVY71_000855"
     CDS             24107..24910
                     /locus_tag="IVY71_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7984925.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24980..25720
                     /locus_tag="IVY71_000856"
     CDS             24980..25720
                     /locus_tag="IVY71_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7984926.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25731..26735
                     /locus_tag="IVY71_000857"
     CDS             25731..26735
                     /locus_tag="IVY71_000857"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7984927.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26780..28624)
                     /locus_tag="IVY71_000858"
     CDS             complement(26780..28624)
                     /locus_tag="IVY71_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984928.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9042 a         5070 c         5902 g         8667 t
ORIGIN      
        1 gctaataccg agggtcagct gattgaacac cgtttaccaa gaggttgtga gcctgccgtt
       61 taactctgag tcacaaggaa caatttcaaa aaacagcttt tcatgtttga tgaatattgt
      121 gacttgtgac cgaggagttg gtaggagaac accgtttacc aagaggttgt gaaaaagctg
      181 ttctgcatca agtaataaga agagccaagt aaaaacagtc tcttatgttt ttaacttggc
      241 tggacttatc accgtagatg taagcttttg aacgccgttt atttgcaaca aaaggggctg
      301 tgacacgatt tttgtcacag cccttttttt gcgaacattc atttagcaat ttttagaaac
      361 ttctgccgcc accgccaaaa gtcccgccac cgctagaatg ggtagtactt ccgccaccgc
      421 cgcttccagg aggtgggctt ttggggattc ttcttgtcgt gacaaaagaa tttgtcagac
      481 gatcggtttt ttcagttaat ttgatactgg atttttctcg gaatggatat ttgtagatgc
      541 ctgatttcaa ttgataacgc gaaacagtca caaaataaaa gacaagacta aggataagag
      601 ccaaaacaac agcaatgatg atttctactg tcgtcaaaac cttatagcgt gtaattttgc
      661 cagtttcttc gtctacacga taatgtcctc ctggaactcc gtcttccaca tatgttgctg
      721 atttagacag gtaagctttt gcagcattgt aataggcagc atttgtcatc tgatcataaa
      781 catcatctaa gatcgaatcg attctgctat cagtcaaata atcgatcata ttaccagaag
      841 tcgaaatgta gatttcacgc tgtcccatat ctaatagcaa tactgagcca ttttgatcat
      901 ttccgactgc gtctcttaag taattatcag caaagtctct tggttcttct gaattagatg
      961 tagtcgttac gatcaagact cgtcctttga ttttttcatt cagcttttct gcctgttgtt
     1021 ccaaagattg gatttgatct gttgtgaata acccagcctc atcatttacc gtagctgtat
     1081 ctgcatgagt agccaccggc cagccaacac ttactagcaa aagaaagaat aaaaaaaatt
     1141 ttttcatcat atcaagtaac ctccgatcag aaagaggatc gctagcccag cgaaaatccc
     1201 aagagcggtc agtcccaatt ttttctggct gatcggcagg acaccgctga cctttcctgt
     1261 ttgtccattc attgcgtagt agtagacttt tttacttgag tcattgcttc gataagtaac
     1321 aagccatacc ggcagtaata cgtaattatt tacttcgttt gtgattgaag cagaagttcg
     1381 cacaccggtc aatgttgtat atccatttgc ggtatctttt aacagcttct ctgaatagtc
     1441 ttttaattca gattgaatct gtgatttgat tgcctcatat tcgatatctc ttttttctgc
     1501 ttggaacccg gctaaatact ggcttttaaa atcaattgct ttatctaacg ggaaaggttg
     1561 cacgccttcg accatctttt gttgcgtatt ttttgataag gcatttttta ctagttcttt
     1621 aaaagaaagc ttgcctgctc ttgaaacagc aaattgcttc gtttctgtgt attcgatgtc
     1681 acctatccgc catatgcgga taactgtacc attcgcctgc atggcaccat ctacttcagc
     1741 atctgtagcc caataaggga aatacacacc tgtcaactta tcgatttgat ccttattgaa
     1801 aaaagctttg ggaacaaacc attttttctt ggtccaagcg agaaatctgt tgattgcttc
     1861 ttctttctcg acagcaaaag gaagtacttt atttggcaag aattctccgc ttaaacgtcc
     1921 cgatagtacg acgggattat ggcagtagta acaataagtc gcagcagttg tcgcatcagt
     1981 cacgatctct gcaccacagt tgggacaaag aaaaagatct atcgctgcgg tattatctgc
     2041 agattcgtcc gctgtatctg aaaaaccgcc tgcctcgtca accgctttgc gctcggcttc
     2101 attcatatcg gcaagctgtt cttgggctgt aaaagtaaaa tcttcggtag gtgaaggttc
     2161 ttctttgctt tcttcgccag ccatacgcgc ttctttttgc ttttgctcat attggctgac
     2221 ttcttcttct gtatagatat tcaggcagta ttcgcaatga aatttttggt ctttgggatc
     2281 aaaagtcaac ggaccgccgc aattaggaca tttatgtgta agaacatcca tcagctgcac
     2341 acctttctaa ataaattaat taacggggat tcctttaaac ggtttaccac agtttggaca
     2401 gaatttaggg atgccgttgg aaagatccac gatctcattg cattcactgc atcgcatttg
     2461 tagcttaggc tgaacgtttt ctacaggttt ggcagctccg cagttggaac agaattttcc
     2521 ggaattttct gtcccgcaaa ctggacatgt ccaagtatcc gcatttcctt gtgcagtttg
     2581 ttgattttgt ttttctgctt gttgtttgat ttgttcttgg ttattttgag ctgcttgaga
     2641 aagataagaa ccatttgcat tcattcccat acctacaccc ataaagccgg tcattgctcc
     2701 tgcttcattt tttccggctg cttccatacc acgtgcaatg gaaccttgca cgtagccttc
     2761 acgtacgcta ggatcaccta gcatcgcccc ttcgttacgc atattgatca gtttcacaga
     2821 atcatcggtg taagaaatgc tggctacagc cacagaaaca atctccatcc cacgtaattc
     2881 acgccaagaa tcatccaatg cagtatccat gtatttgctt aattccaaac tcttagatgg
     2941 tacataagaa atccgttgac catctgcaga catctgattg attgctgatt gtagcgctgt
     3001 taggaattca gctaaatact gctcattgat atcattgatt tccacttgtg ttttgttttt
     3061 agggattgcg tttgtataaa ataaaatcgg gtcaacgaca tgaatagaat aagtgccatg
     3121 tgcccgtaag aataattctg cgttatagaa attgtcaaag tagttcaacg gcgcgcttgt
     3181 accgaatttg attcccttta tttcttggag attgatataa aaaacttgtt gtttctgcgg
     3241 tgtgacgccg ccaaatttga atcggtcaaa agtttcagca attgcttctt tcagtgttcc
     3301 attgaacatg gaaggagctg cgtcattttt aatggtgtaa taaccttcct cagcggtata
     3361 atcgatgatt tttccaccat cgaccaaaag catcatcatg tttggataga catggacgac
     3421 tgttccgtct gttaaaacat cttctgttcc tttacgattg gagccacgtt tgtcatcttt
     3481 gcggactttg acaccttttg tcatgaccgt tgtatcactc atattgtctg gttcgatgac
     3541 ttctagccat tgatctgcca gaccgccgcc aaccatactt gttgctgctt tgataagacc
     3601 cataaaaaat tcctcctctg attcagctag tgccaaacaa ttattgttta tttattatac
     3661 cataggaaga tgtgaaactt ctcggactag agacggaatt gaaatggaaa attgcgtatt
     3721 gtttaaatac ctgaaaaagc cgagaaggat agtagttttt ttacctaata tcacgtataa
     3781 taagagatag tgaatttttt aaaaaagggg gatccttatg gcatatcaag cactttatcg
     3841 agtctggcgt tcgcagcgtt tcgaagacat cgttggacaa aaagctgtga cacagacact
     3901 aaaaaatgct attgtttccc acaaaacctc acatgcctac ttgtttacag gacccagagg
     3961 aaccggaaag acgagtgcag caaagatttt cgcaaaagca atcaactgtc cgaacagtaa
     4021 agacggtgag ccatgcaacg aatgcgaaat gtgtcgatca attactgctg gtacgcaaga
     4081 agatgtgatc gaaatcgatg ctgccagcaa taacggagtc gaagaaatcc gatttatccg
     4141 tgatcgggca aattatgcgc ctacaaaggc agcgtacaaa gtgtacatta ttgatgaggt
     4201 acatatgctt tcaacaggag ctttcaatgc cttgttgaaa acattggaag agccaaaaga
     4261 aagtgtcata ttcatcttag caacgaccga acctcacaaa ataccagcta cgatcatttc
     4321 acggactcag cgatttgatt ttaaacggat caatacggca gatattgtcg aacatcttga
     4381 atttatcttg aaagaatcga ctatcagcta tgaaccagct gcattgaatg tgattgcccg
     4441 ttctgcagaa ggcgggatgc gggatgcatt gagtattctt gatcaggcaa tctcgtttag
     4501 tgaaggaaca attactttag atgatgccat gcaagtgaca ggtagcttaa ctgacgaaat
     4561 gatggacagt tacttgagtg cttgcgtgaa aaaagaagta ccagaagcat tggaaacatt
     4621 gaaccaaatt ctcgcagccg gcaaagaatc acgtcgcttt ctagaggata tgttacaata
     4681 ctgccgtgac ttgctgatgt atcagcaagc acctaaccta gttgaggaac aatcgagtac
     4741 gatcagaagt acatttacag aattggcaca aactgtttca gcggatcagc tgttttcaat
     4801 gataaagatt ttgagcgata ctcaaaatga aattcgtttt accaatagtg ccgctattta
     4861 tttggaagtc gctacagtca aacttgcaca gctaccgtct gcttctgttc cttcagcaga
     4921 tgtagcattt gcagatgatc agatagtaaa acagctacaa caagagatta aggaactaag
     4981 aaacgaaatc aaggaactac gcagtaatac aggaattgag aatacaaaag tacagccggt
     5041 acagcaaaca aatcataaaa aacagacaag tacctatcgt gtaccgacgg aacgagtata
     5101 taaagttctg cgggaagcga caagacagca tctgaaccaa gtaaaagaag tatgggaaga
     5161 cttgctgatg agtctatctg tcactcagcg agcaatgctt aaagcaagcg aaccagttgc
     5221 agctggacct acaggactag tgattgcgtt tgattatgag attgtttgtc aaagagcagc
     5281 aaatgacgaa gaattgcagc tttcggtaca caacaactta agccgaatga tcaaagatta
     5341 tgcgcctgat cctgtttata ttacaagaga aagctggcca gaacttcgaa gaaattattt
     5401 gacgcaggca caagaagagg gaacagaaaa ctcttttgct tctgaagact cagaaggaga
     5461 aatcgagttg cttcctcaag aacaagaagt accagaagaa acacttgtag taaaagcaga
     5521 ggaactgttt ggctccgaag ctgttacagt ggtagatgat taataaaaag gagtagataa
     5581 ttatgatgcg tggaatgggc aatatgcaag gcatgatgaa acaagtacaa aaaatgcaaa
     5641 aagaaatggc agaagcacaa gagctgctaa ataaaacaga gtttacaggt aaagccacca
     5701 atgaattggt caaagcggtt tttacaggcg atcgtcgcat gaaagatatc caaatcaatg
     5761 aagaagtcgt tgatcctgat gatatcgaga tgcttcagga tcttgtcgta atggcagtca
     5821 atgatgcgat cgcacaaatt gatgtggaaa ctgagaaaaa attagggaaa tacgcaaaag
     5881 gtttgccagg attttagttt taaactgaag gagtaatgaa tatgcaatat ccagaaccaa
     5941 ttgcgaaact gatcgacagc tacatgaaac ttccaggaat cggtcagaaa acagcgacaa
     6001 gactcgcctt ttatacgatc gatatgaaag atgaagtagt aaatgaattt gctaaatctt
     6061 tgttaagtgt aaaacgcgat ttgcattttt gtagtatctg cggaaacatc acagaagaag
     6121 atccctgcga aatatgtaga aatccttcac gtgatcgaag cgttatccta gtggtcgaag
     6181 agccgaaaga tgtaatggca atggaaaaaa tgcgagaata tcacggactt tatcatgttc
     6241 tccatggtgt gttatcaccg atggaaggga caggtccaga agacatcaat atttcttctc
     6301 tgttgcagcg gctgcatgat gcagatgtaa aagaagtgat tatcgcaaca aatgcaacaa
     6361 cagagggaga agcaacagct atgtatctct cacgtctgat caaacccgca ggtatcaaag
     6421 tgactcgtct agcacatggt ctgtctgtag gctctgatat cgagtatgca gatgaagtta
     6481 ctttattgaa agctgtcgaa ggaagacgtg agttataaat ttctaattaa gtgttttttc
     6541 agatacaata taaaagcagc acaagagatg ggggcaacaa tgtgaacgga ctatttatta
     6601 caatcgaggg gcctgatggt gcaggaaaaa ccagcatctt gaatgaactt taccctcttt
     6661 tgaaagaagt agcaaaaaca gaaatcattc aaacaagaga accaggaggc attccgattg
     6721 ctgaagagat ccgagcagtg atccttgatc caaaaaatga tcggatggat gaacgtacgg
     6781 aagcactgct ttatgcagca gcaagaagac aacatctagt cgaaaaggtt ctgcctgctc
     6841 tggcaaaagg gaaaatcgtt ttatgcgata gatttgtcga cagttctctt gcttatcaag
     6901 gtgcaggccg acgcatcggc gttaccgaga ttgctcggtt gaatgagttt gctacagaag
     6961 gtactacgcc ggattttacc ctttatcttg acgtagattc agacaccgga ttaagaagaa
     7021 tcaagaaaaa cagacaaaat caaatcgacc gtttagattc ggaaggtttg gaattccacc
     7081 agcgtgtacg tcatgcttac ttaaagctgg cagaagagaa tccggaaaga atccacaaag
     7141 ttgatgcaag aaaaagcttt gaagaagtgc tgcaaacaag ttatcataca attattgaac
     7201 agtatccgca attttttgaa aactaggaag gtgaacgttt atgaaaatta ttttagcgat
     7261 tatccaagac aaagacagca accgtttatc aaatgaattg attgatgcaa atatccgtgc
     7321 cacaaaacta tcatcgacag gcggcttcct taaggctgga aacagtacgt ttatcgtggg
     7381 tattgaagat gaccgtgtag aagaagcatt ggaaatcatc aaaaaaacgt gtgaatcaag
     7441 aaagcaattt gtatctacac ctgttacact ggatatttcg atggatgggg gcgtccctta
     7501 tccagtagaa gtagaagtag gcggtgccac agtcttcgtc ttacctgttg aaggattcca
     7561 tcaattttag gaggagctga gctatgaatc aagaaataaa cttggctcaa atgcaaccga
     7621 tcgtgtatca gcagcttcaa cggagttttg agcatgggcg tatcgctcat gcttatcttt
     7681 ttgaagggga gaaaggaaca ggaaaacatg aagtaggtat atggcttgca cagcacctgt
     7741 tctgtaccca aatgaaagac aatctgcctt gtggaaaatg caacaattgt cagcggatcg
     7801 aaaagaaaga acatccagat gttttggtta ttgaaccgga aggacaaacc atcaaagtcg
     7861 atcagatcag acgtttgcag acggaattca gtcgcagcgg ttacgaatca agaaaaaaag
     7921 tttttttgat caaagaagct gaaaaaatga atagcagtgc ggcaaacagt ttgttgaaat
     7981 tcttggaaga gccccctgga gattttttag cggtgttaga aacagatgca gtaggaagaa
     8041 ttctccctac tattcaatct cgttgtcaag ttctgcattt tcaagaactt tcgaaagaag
     8101 cactgatcca tcgattgcaa gaagagcaga ttccattgga aaaagcaaaa cttttggctt
     8161 tcttaacaaa cagtttagga aaagcagttg aaatctctca agatgaatgg tttaatgatg
     8221 ctaaggactt gatttatcaa tggttcgttt acttgcaaaa gcaagatact caagccttta
     8281 tctatgtcca aaaaaaattg gtaaaatcat ttaaagaaaa aacacaacag tttacaggat
     8341 tatctgtctt gatgttttat tatcaggaag cacagcgtca agcagtagca aacggcgaat
     8401 atggcaaagt gaaacagata aaccagataa ttgaacggat attgatcgca gagcagaaac
     8461 tacgcagcaa tgtcagtttt caggctgttg cggaacagtt cgttttgcat acgatttttt
     8521 agcttgccgc aaaggatgat gatcaaggag gatggaaatg gtagaagtag taggtgtccg
     8581 atatagagat gcaggtcata tctattattt tgcaccagga gactctgaat atgtttatag
     8641 tgacaaggta ttagtcgaat ctcagcaatc cttacatatt gctactgttg tccttcctaa
     8701 aaaagagatt gatcccaatg accttcctga agctatccgt ccgatcttgc ataaagcgtc
     8761 acaagctgaa ttagaaaaag acaaaaaaaa caaaaaagat gcttatgatg cattgaaaat
     8821 cgcaaaagaa aaagtgcagt tgcgtgagtt ggaaatgaaa atggtcaaag ccgaatacac
     8881 atttgatcga aataaattga ttttttcttt cacagcggac gggcgaattg actttagaga
     8941 attggtaaaa gatctggccg ctgtatttcg tactcgtatt gagcttcgac aaattggtgt
     9001 ccgcgatgaa gcaaagataa taggcggaat tggtccatgt gggcgtccat tatgctgttc
     9061 ttctttttta ggcgatttcg tgcctgtatc aatcaaaatg gcaaaagatc aaagtttatc
     9121 tttgaatcca acaaaaattt ccggtttatg cggacgcttg atgtgctgtc tgcaatatga
     9181 gagcgaagaa tacgagacag cgaaaaaaga actccctgac tttggaaaag taattgctac
     9241 acctgatggt aaagggaaag tcgtagggtt gaatctgctt agccgtatcg ttagtattcg
     9301 gctgactgga cgggaaaatc ctgtcgaata tgaatgggat gaattgaaag aatttatcca
     9361 agtaggtgag atgaatggat aagagatcat tatacgacgg attgaatcaa ttagaatcag
     9421 agttgagaaa tacattaaca gaacttgttg aaatgaagca tgctttgcat gagatcgtag
     9481 aaaaaaatac aactttggag atggaaaatc aacgtctgcg ggaacacctg cgggaattga
     9541 accaagttgc acaagctcca gctgaaaatg tgaagcagga actttctaag tctcgtatga
     9601 atcttgaaaa aatttatgaa gaaggattcc atgtctgcta cgatttatat ggatcaagaa
     9661 gggacaatga cgagccttgt gcgttttgtc tagaagtgat ctatcgcgaa agcggtcgat
     9721 gaccaataac aagaggagac tggagcggag aaatactgct ccagtctttt tattaaggag
     9781 attgtgacag atgtataatc aaaaaagttt caagggaaat ccggtatatg gggatttata
     9841 tcttgtaccg acgccaattg gaaatttaga agatatgagc tacagaagtg ttcgtatgct
     9901 gcaagaatcc gatcttatcg caagtgagga tacgcgtaat acacaaaagt tattgaatca
     9961 ttttgaaatc catacacccc aaaaaagttt acatgaacat aattataaag aacgtgtccc
    10021 tcaattgatt gctttgttaa aagaaggaaa aacgattgct caagtcagtg acgcaggaat
    10081 gccttccatc agcgatcctg gacatgagct agttttagcc tgcataaaag aagggatttc
    10141 tgtgatctct atcccaggac ctacagctgg gatgacggct ttgattgctt cagggctagt
    10201 gcctcaacca tttttatttt atggcttcct tccacgtaag aaaaaagaac agaaagaaac
    10261 attgaaaaag ctaaaagaac agacagttac gctgattttt tatgagtcac cttatcggat
    10321 cactgctaca ttgacgaatt tcttagaaat ttttggaaat cgccaagtcg ttttgtgtcg
    10381 ggaattgaca aaggtccatg aagaatacct tagaggaagt acggaagagc tactcgctta
    10441 tatcgaagaa catccagtaa agggtgaatg ttgtctgctt gtagaaggga atttgttcga
    10501 aatgccagtg acggaagttg atgaagagtt gggaacgtta aaggaacaag tagaagcaaa
    10561 gatcgcttca ggcgaaaaac caaatgctgc tatcaaagca gtagcgctga aaaatgggct
    10621 gaaaaaacaa gaagtctacc gtcagtacca tgaattagac tgagatagta taagttgtct
    10681 ataatgaaag actaatgagg aaatcagcta ctaacttttc aaatctcttg aacgcgaacg
    10741 aatgttcttg tataatgaat aagaggtgag gatgatggag ctgcaatttc ctttacataa
    10801 tgatacatca agaaaaatca ttcatattga tatggatgct tttttcgctt cggttgaaga
    10861 acgagatcat cctgagtttc gcggacatcc attagtaatt gccagacacc ccagtgacac
    10921 tggtgggcgt ggagtagtga ctacagcaaa ttatgaagca agaaaattcg gaattcattc
    10981 agcaatgagt gcacaaaaag cgtatgagct ttgtccgcaa gccatcttta agcctggcga
    11041 ccatcataaa tacagcgata tttccaaaca agtacgggaa gtattctatc gttatacaga
    11101 tattgtggaa ccagttagta tcgatgaagc gtatcttgat gtgaccgaaa ataaaatcaa
    11161 ttgcaagtca gccatcaaaa tcgcccgaat gatccaagca gatatttggg agagtgtcca
    11221 gctaacatgt tctgcgggag taagttacaa taagtttttg gccaaattag catcagacta
    11281 tcaaaaacct agaggaatca cggtagttgc ccctcaagac gctgtgccat ttttaaaagc
    11341 tttgccaatt gaaaaatttc atggggtagg gaagaaaact gtacctagaa tgcatgagct
    11401 agggatttat acaggaaaag acctatatga atgcacagaa atgatgttga ttcgaaattt
    11461 tggaaaaatg ggctattctc tttatcgtaa agtcagagga atccatgatt ctccagtaaa
    11521 cgttacaaga gagcgaaaat ctgtgggaaa agagcatact tatgggcagc cgttgcaaac
    11581 ggaagaagca gtgctgactc agttgcgcca actagctgaa aaagtagaag aagcactgag
    11641 aagagtccaa aaacatggga aaacagtagt tttgaaggtc aggtacacgg actattctac
    11701 tgtgaccaaa agagtgacat tgccagaata tatctataaa aaagaagcac tcttttatca
    11761 agcaagcctt atatgggagg aaatcttggg agtagaaaaa ggaatccgat tgttagggat
    11821 tactttgacg aatctggacc cgatgactta tgaaaacatc gtccttccat tatgggaaaa
    11881 tcaagaaata taaaaacagc tttgagagtt gaaaccggaa atccgttttc tctcccaaag
    11941 ctgtttttat atcaagtctt gctttttgat ttgttcatag gtttctgttg catccgtaca
    12001 tttttcccaa atcacaactt gattggattc taaagatttt tggacgtcaa tgatccgctg
    12061 gttgctactt ccgcgaaatt gtagttttag gtcacgtttg cttaattcaa agcgtccgtc
    12121 aacaaggata tcgatttgcg aaaggagttc taatttatct tctgaatctt gtagcaattc
    12181 atcaaaggta tagcctgtcc atgaccagat atcttttgtc gtaccgaact cttttcggat
    12241 acgtcgtacg acttgcagac aaacatctgt attcaaaaaa ggttcgccac caagtaaagt
    12301 taatccttgc acgtaatcgt tgcgcaagtc agcgatgatt tgttcttcta attcaggtgt
    12361 gaatggtttg ccatatcgaa aattttgtgc agctttgttg aagcagcctt cgcaggcaaa
    12421 taagcagcca ctaacgtaaa gactgttgcg gacaccttca ccatcgacaa aattaaacgg
    12481 tttgtagtca gcaatatagt tttggctgta gtcttttgct aaccattctt ttggttttgg
    12541 atttctcata aaaaaacttc tttcatttaa ggttcaaaga tatagctatt gaagataaaa
    12601 tctactcgac cacataaata agaacaaact gacctaaatc cattgttcaa gaaagcttgt
    12661 tcttattttg atagagggcg ttcaaagata caaagccatc gaaagagggt ggtgaaaaaa
    12721 gacttgtcat agcctcttat tctgaataaa cggtgttcaa aagcttgcat ctgcagtaat
    12781 aagcccaact aaacgaaaaa tatgaggaac tatttttgtt tagctattct tattacttga
    12841 tgcaggacag ctttttcaca acctcatttc atgtgtttca cacgtgagga aatttcttta
    12901 tggcggccat gtaccatcgg acgagcttgc ggatttccta aatagccaca agtacgtttg
    12961 actacatcac atgtttttgg atcatgattt ccacattctg gacattcgaa ccctcgttct
    13021 gtcggtttga aatcaccttc gaaaccacat tgatagcaat gatcgattgg cgtattcgta
    13081 ccaagatacc cgattttgtc atatgcaaaa tcccatacag cttctaaagc tttcggattt
    13141 tgttggagca tagggtattc acagtagtgg atgaatccac ctgaacaata ttttggataa
    13201 tccttctcaa attccaattt ttcaaatggc gttgggtttt tacgcacatc atagtggaaa
    13261 ctgtttgtat aatattcttt atctgtgata ttttcgatga tgccgaattt ttctgtatcc
    13321 aaacggcaga aacggtcagt tagactttca cttggagttg aatagacact gaaatgataa
    13381 ccatattcat ccccccatgc atctgcatta gcttttaatt ctttgacgat atcaagtgtg
    13441 aaagcttttg cttctgggtt tgtttcccat tcgccaccga agaaagctga agcaacttca
    13501 tacaatccaa tgtagcctaa cgatacagta gctcgacggt ttttgaataa ttcattgaca
    13561 gaatcggtac gtgaaagacg tttcccgaaa gcaccataca tatataaaat tggcgcattt
    13621 gctggaatgg cttctttaca acgttctaca cgatagagaa gcgcatcttt catgatttct
    13681 aagcggtcag acagtaactg ccagaattta ttctgatctc cttttgattc gatcgcgatt
    13741 cttggcaaat tcaatgtaac aacacctaaa ttcattcgtc cgacatttac ttcttgacca
    13801 ttttcgtctt tccacccttg caagaatgaa cgacagccca taggtacttt gaaacttcca
    13861 gttaattcaa cgattttatc gtaattcaga atatcaggat acattctttt cgttgcacat
    13921 tccaatgcta gttgtttgat gtcgtaatta ggatcttcag ggtttaagtt cacacctttt
    13981 ttcaaagtga agatcagttt agggaagata gctgtccgct tttcactccc taatccattg
    14041 attcgaatct gtaaaattgc tttttgaatt tctctttcaa accagttttc tcctagtcca
    14101 aatcctaatg aagtgaaagg agtttgtcca tttgaagtaa ataaggtatt gatttcatat
    14161 tccaaacttt gcatagcatc gaagatatct tttttggttt ttgcttttgc atattctttt
    14221 tggcgttctg gcgaatcgat ccattcttcc gcgtctttta gatgtttttt atagttcaat
    14281 tctgcaaaag gagcaagcaa ttcatctgtt cggtcggcag aacagccacc atattgactt
    14341 gacgctacat tggcaataat ctgtgagatt tgtgctgtgg cggtctggat agattttggc
    14401 gattcgactt cggcattgcc gattttgaac ccattgttca gcatgccttt gaaatcaatc
    14461 aagcagcaat tcgtcatcgg tgtataagga tgataatcca aatcatggta atggatatcg
    14521 cctttttgat gcgcatttgc tatatgtggt ggcaacattt tcagtccgat tgattttcca
    14581 acgatcccgg ctgtcagatc tcgctgtgta ttgaaaacat tactgtcttt atttgcgttt
    14641 tcgttcacga cagtttggtc cttgttgatt agcttttcaa tcgaaaagtt gatatctgtt
    14701 gctttactgc gagcaaaatc tcgtttggta cgataattga tgtactcttc tgctaattca
    14761 tattcgtttt tttctagtaa tatatgttcc acgatgtttt gaatctcata gattttgaca
    14821 ttttcagcaa accggctttt gatttcttgg acaaccaagt ctgcaatcat ttgaattttt
    14881 tcataagtaa gtggtgataa tgagccgtga attttctttt ctgctttgat cagtgcttca
    14941 ttgatctttt gttcatcgaa atcaacatgg cggccatcgc gtttgatgac tttcatctgg
    15001 gatgtctctg gctgctgagt aacgacagtt ttttcttcat aatttacttt cattatcttc
    15061 aactcctgct aaattgttct tgtctatcat aaaacaaaat cggggaagga gcaactaaat
    15121 atcggctata tatagtattc aatgtgctgt ataattggat ttctatacta tatgtagtgt
    15181 atattttatt gtgaaaaaag gtgaaaactt gtagctggtg gatttgtttt ttgcatattt
    15241 aattttgctg aataaaataa ttgaataaaa ttttttattc agtccaagta aaagtgacaa
    15301 aaacataaga gcaaattatt cgtagatatg ctactatgca aataaataga aatgaaaaat
    15361 gaaagaggag gagaagaatt ggaaaagatg atcgaagtga aacatctaag taaaagctac
    15421 ggcagcaaac tttctcccgt caaagcctta gataatatct cattcacagt agaagaaggc
    15481 gagtttgttg ggattatggg cccaagtggt gcaggaaaga caacgctgat gaatatcttg
    15541 tccacgatca atttgcctac gatgggcagt gtctcgatcc aaggaaaaga aatcacaaaa
    15601 atgaaaaaca gtgagctcag tgactttcgt cgaaaaaaac taggattcat ttttcaagag
    15661 ttcaatttga tcgataccct aaatgcaaaa gacaatatcc tattgcccct agcagttgaa
    15721 cggatgacta aagaggaaat ggaaaagaga gtaagacatg tagctcaatt gttgaatata
    15781 gaagaactgc tcaaacggta tcccgatgaa ctttctgtcg gacagagaca acgaattgca
    15841 gctgctagag cacttgttgt tcagccacaa gtgatttttg cagatgaacc aactggctct
    15901 ttggactcga agtcggctac agaattgttg aactacttga agaccatgaa tcaaaaagaa
    15961 aaagcaacga tactgttagt cacccatgat ccatatacag caagctactg cgatagaatt
    16021 ttatttatta aagatggtgt gattttttct gaagttgtca gaagagggac aagaagagag
    16081 ttctttgaga aagtaataga catgcaggcg acgattggcg gaggaggaaa aatgaatgct
    16141 gtataaacta gccgttaaaa gtttttttcg gcagtcacga ggctatcttg tttatttttt
    16201 cagtttgact ttatccgtaa tgatctatta ttccttcagc gcaatgacct acgatcaatc
    16261 tttagtcaga agagcaagtc aagatacttc gatagatggt ggattgagtt ttggtggatt
    16321 tatcattact gttgttttgc tttttttcgt attcagtgcc aaccgatttt tcttgaatag
    16381 aaggcaaaag gaaataggca tctatcagct ttttggtatg aataaactgc agatatcagg
    16441 tatatatgtg ttagaaatca tgataattgg attgttcgcc tgtatttctg ggatattact
    16501 cggtattatt ttttccaaac tcttttcaat gattttggtt cgaatgatgg atatggatct
    16561 tactagtcca ttttttatct ccattccatc agtagtagat actttatttg cttttttcat
    16621 catcctttta gcagtatctg tatattcaat atggaaaata tggcgctatc caatgattcg
    16681 aagttttgga gaaaaagaac aagcagatag ttcaacaatg cgcttcagga caaggcaccg
    16741 attactcgca gtcatcgggt tactactttt aacgagcgga tattttggag ctagtcattt
    16801 tagggaaatc acttcatgga tgatcagtta tcattatctt gatacattag ttttcttttt
    16861 tcctttttta atttttttcg tttgtgtggt cggtacttat ttgtttttct gttatacatt
    16921 tcggttattt ttgatcatat tgagtaaatg gaagatttat tactatggta tcaatttttt
    16981 gataatgggt aacacgcaag ttcatttgct caaaagttgg cgtacaaatt ctctgattac
    17041 agtcattctc ggaatttctt taatgacaat tggcggcacg gctagtatct ctactatttt
    17101 atcctaccag gagaaagttt cctccccgat ggactatcag tttgatgttg agacagagaa
    17161 aaaaatcaag ccgattctag cagaggaagg gcagaagata actaaagaaa tcaccttgca
    17221 ttataaagtc attgggagta tctatagtca aaatctcgaa gaacctatgt ttcaaactgc
    17281 taatctgatc acggaaacac aatatcaagc cttcagaaaa gttaatccag atttgccgaa
    17341 catttctttg aaatcacctg gtcatactgt tttgcttgat caaatgcaaa ctctttttcg
    17401 gaatatttca ttatatggat ctagtattgg actgccagat aatcaagaat tgaagattca
    17461 acaaatgctt ccaagtgtat taggtgacga aacaatggta tatctaagtc ctgtactgat
    17521 tgtagatgat accgtttttg aaaaagcaaa aggtgtggat tatcaagtag tcaatgtaga
    17581 tgtatcaggt ggaaataatg aaaaaataga tgaacgactg agcaatgacc tgtcgataaa
    17641 gtggctcaat gctgtttatt attcttatga tatcgtaaat ggaagtctac aaggagaaat
    17701 tagcacaaag aaacttccgg atgaccaaat gaaggaagca caatttagcc aagaaagctc
    17761 tcgtttgaat ttctcttcca gctattcgaa attgcgtgca gttaatcggc gaatcggtat
    17821 caatattttt gtcacactat ttgtcgggat gatcgtgatt attgctactg gtagtatttt
    17881 gacagtccgg caattagcag aagcagagga agaaaaagaa aattatcacc tattgactaa
    17941 gctggggatt ccacaaagaa ggatcagacg gatgatcttc gagcaaaacg cacttacctt
    18001 ctttccaccc atgatattag ggatcactca cgcagtattt gcaatcaatg tcttcactca
    18061 gtatataaaa actgccgatt actggttggc ttatcttgtg agtggcttat tgatccttat
    18121 ctatcttttg ctttatgtca ttacgagtca gttatattgt cggattatcg aggaatgata
    18181 tacggaataa gacaagataa taaatagaac taaattttga ataccattta tttggaataa
    18241 gaggttgtga cacgattttt gtcacaacct ctttattttt tatatttttt aaaagtacga
    18301 gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc tatgtgacaa
    18361 aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt gtcaaaatta
    18421 gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg cgttctaatc
    18481 caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt gcagtactgc
    18541 tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt atctataacg
    18601 aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga tgattgggga
    18661 ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt tggtgaattg
    18721 aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg cgatggacca
    18781 attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg ggttgtcaaa
    18841 tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat cctatcgatc
    18901 tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga agatattcct
    18961 tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc agtaggattt
    19021 tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac ttctggtact
    19081 actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt tgttcaatgg
    19141 acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc gccattttct
    19201 tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat gctgaagccg
    19261 ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc agccttgaag
    19321 ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga acctacattc
    19381 aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga attgacgaaa
    19441 aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa tacttacggt
    19501 ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt actagatagt
    19561 tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat tgtccaagaa
    19621 ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc aagcgtatca
    19681 aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca aaatgactta
    19741 gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt attttatgaa
    19801 ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg ggatatcgaa
    19861 cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa ataccaagga
    19921 acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa acagccggat
    19981 tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga ttatatgatt
    20041 cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa aatcgatcga
    20101 aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc ccttatgcag
    20161 agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta tctttattga
    20221 tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt ttatttgtca
    20281 gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt ttgtggcaga
    20341 cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca ggatggtttt
    20401 ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact cctttttttg
    20461 aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc tttaaatctg
    20521 tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca tttcgttata
    20581 tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc tataggagat
    20641 ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt ttgggaaaag
    20701 gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat tattttggtc
    20761 aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt tcatgggcat
    20821 tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct ggctatagct
    20881 tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat ttcaataaac
    20941 cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg ctgtcctttt
    21001 ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa aaatttttta
    21061 aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta atgggtatat
    21121 ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt ctgatctgtc
    21181 tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct tctaacaaat
    21241 ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc ttcctgattt
    21301 tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat taatttatat
    21361 aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag aaataacagg
    21421 aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt tgcttgattc
    21481 tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc aagtccctgt
    21541 atctgaattc gagcgagagg attggtgcac accgaataaa atcattcaac aagcaactgc
    21601 attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt tgttttagct
    21661 gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc taatccagaa
    21721 acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga tgcgataaaa
    21781 aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga attaagcaga
    21841 atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta tcgtccattt
    21901 ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg ttctgcagga
    21961 gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt cgttaaaaat
    22021 ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata cgactggttg
    22081 ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt attaactttc
    22141 tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa aaaagggaag
    22201 ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct gaagagagag
    22261 gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga tcatgagtca
    22321 aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa tcaaattgga
    22381 gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac gcatcgtatc
    22441 cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg tttttcgcca
    22501 gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa cgaagtattg
    22561 ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc tcaagaaatg
    22621 ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt caatcgaata
    22681 gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat ccatctaggc
    22741 tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga aaatcatatt
    22801 actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca acatcaaata
    22861 ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt gttttcttta
    22921 tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa aaatcataag
    22981 caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat acaaaagaag
    23041 aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa gaagaggtaa
    23101 gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat gacggtttag
    23161 gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt tctggacata
    23221 tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg gaattccaaa
    23281 ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc aaaacacgtg
    23341 aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta acacctcaag
    23401 taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca tcaagccaag
    23461 aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat attgaataca
    23521 aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt atcgggacag
    23581 ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat gttttattcg
    23641 caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt acacaccttg
    23701 taaatcccga tattgctttt gctttagata ctggtacagc aggagacact ccaggaatga
    23761 caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt tttgatgcat
    23821 ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag gaaatggata
    23881 ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa atgcatttga
    23941 caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg cattcccata
    24001 catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca gaattagtga
    24061 aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga ataatagaat
    24121 acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat ggggaatcaa
    24181 tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact tttttgacgg
    24241 tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc tagtcgataa
    24301 tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag ttgtacgggg
    24361 tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt ttggttcgat
    24421 acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt taatctttct
    24481 gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca gttcaaatgc
    24541 ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt tatcgatgat
    24601 agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat tccaatttgc
    24661 tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga aatatggctg
    24721 gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt ttatcggctg
    24781 gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg tttatgtgaa
    24841 tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc cagacgccta
    24901 ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg taggataaat
    24961 ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag cgtatcctta
    25021 taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt tgttgatcga
    25081 tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg atctagatgc
    25141 tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat tacagtatta
    25201 ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg gacacacaaa
    25261 agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac atccttattt
    25321 tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa caatccatcc
    25381 agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat tcgtatttac
    25441 aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg atttgtttct
    25501 agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca cttcaaaaga
    25561 agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac acctacctca
    25621 gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca aagggattga
    25681 tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac atgatttttg
    25741 taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat ttgctgcaag
    25801 aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca atcatcatcg
    25861 gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa aatggcattc
    25921 atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga aacctgaact
    25981 ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa gtaacacagc
    26041 caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa ggccgaaatg
    26101 atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc aacggtcgga
    26161 tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc aatgcattaa
    26221 aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct cgtgtaacga
    26281 atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa tttcgtcaag
    26341 aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg tacgaattga
    26401 ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga aattgggatt
    26461 ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt tctatcggtt
    26521 cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa atctttggcg
    26581 atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga cagaaatatg
    26641 ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat tttggtgcga
    26701 tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg gaatccacat
    26761 atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt tgaacagtga
    26821 tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga tccgctacgt
    26881 ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg atgcctcgac
    26941 taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa tgttcctctt
    27001 gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca tgagtttctc
    27061 taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg ttgataggaa
    27121 gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca gctaacttct
    27181 ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac agcatttcga
    27241 aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt tggtccgtat
    27301 gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt gtatttactt
    27361 cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt ttgattttta
    27421 agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt agattgtagc
    27481 tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc gttgtttgat
    27541 agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc aagacgatcc
    27601 aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg catgcaagtg
    27661 aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat tcaggaagtt
    27721 ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt tggaacgtac
    27781 aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag cagatgatga
    27841 aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat ggttcaaaaa
    27901 gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa gcaaagcaac
    27961 ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag atcgtataga
    28021 aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc ggcactgatt
    28081 gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaactttc ggtggcagtc
    28141 cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg atataatttg
    28201 ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga taatctttcc
    28261 gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt cctttgatta
    28321 gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat gtttcgtctt
    28381 tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca ggatcatctt
    28441 ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc gcaggagaaa
    28501 aaataaaata aaagaaataa gcaattcctg ttaaaaataa caatgataat ataatgatta
    28561 tgctccactt tatacgtaaa taaaaagatt cttttttatt atatatgtta ttaaaaaaag
    28621 acataaaggt attcctttct aaaacttgta atgttagggt aaacgttttt taaactaacg
    28681 t
//
LOCUS       DACCXW010000024        28109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCXW010000024 DACCXW010000000
VERSION     DACCXW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.024"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..163)
                     /locus_tag="IVY71_000859"
     CDS             complement(<1..163)
                     /locus_tag="IVY71_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP7984929.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIER"
     gene            complement(182..1222)
                     /locus_tag="IVY71_000860"
     CDS             complement(182..1222)
                     /locus_tag="IVY71_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7984930.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            complement(1238..2629)
                     /locus_tag="IVY71_000861"
     CDS             complement(1238..2629)
                     /locus_tag="IVY71_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7984931.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            3007..3990
                     /locus_tag="IVY71_000862"
     CDS             3007..3990
                     /locus_tag="IVY71_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP7984932.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(4358..6496)
                     /locus_tag="IVY71_000863"
     CDS             complement(4358..6496)
                     /locus_tag="IVY71_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7984933.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(6535..8121)
                     /locus_tag="IVY71_000864"
     CDS             complement(6535..8121)
                     /locus_tag="IVY71_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7984934.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(8111..9331)
                     /locus_tag="IVY71_000865"
     CDS             complement(8111..9331)
                     /locus_tag="IVY71_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984935.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(9343..10149)
                     /locus_tag="IVY71_000866"
     CDS             complement(9343..10149)
                     /locus_tag="IVY71_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984936.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(10359..11639)
                     /locus_tag="IVY71_000867"
     CDS             complement(10359..11639)
                     /locus_tag="IVY71_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7984937.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(11683..13716)
                     /locus_tag="IVY71_000868"
     CDS             complement(11683..13716)
                     /locus_tag="IVY71_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7984938.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(13750..14601)
                     /gene="rfbD"
                     /locus_tag="IVY71_000869"
     CDS             complement(13750..14601)
                     /gene="rfbD"
                     /locus_tag="IVY71_000869"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7984939.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(14699..15727)
                     /gene="rfbB"
                     /locus_tag="IVY71_000870"
     CDS             complement(14699..15727)
                     /gene="rfbB"
                     /locus_tag="IVY71_000870"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7984940.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(15749..16321)
                     /gene="rfbC"
                     /locus_tag="IVY71_000871"
     CDS             complement(15749..16321)
                     /gene="rfbC"
                     /locus_tag="IVY71_000871"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7984941.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(16335..17201)
                     /gene="rfbA"
                     /locus_tag="IVY71_000872"
     CDS             complement(16335..17201)
                     /gene="rfbA"
                     /locus_tag="IVY71_000872"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7984942.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(17301..18035)
                     /locus_tag="IVY71_000873"
     CDS             complement(17301..18035)
                     /locus_tag="IVY71_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7984943.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            complement(18013..18840)
                     /locus_tag="IVY71_000874"
     CDS             complement(18013..18840)
                     /locus_tag="IVY71_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7984944.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(18840..19640)
                     /locus_tag="IVY71_000875"
     CDS             complement(18840..19640)
                     /locus_tag="IVY71_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7984945.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(19633..20772)
                     /locus_tag="IVY71_000876"
     CDS             complement(19633..20772)
                     /locus_tag="IVY71_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7984946.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            20812..20970
                     /locus_tag="IVY71_000877"
                     /pseudo
     CDS             20812..20970
                     /locus_tag="IVY71_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(20977..22170)
                     /locus_tag="IVY71_000878"
     CDS             complement(20977..22170)
                     /locus_tag="IVY71_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7984947.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(22249..22455)
                     /locus_tag="IVY71_000879"
     CDS             complement(22249..22455)
                     /locus_tag="IVY71_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7984948.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(22448..23590)
                     /locus_tag="IVY71_000880"
     CDS             complement(22448..23590)
                     /locus_tag="IVY71_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7984949.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            24102..24737
                     /locus_tag="IVY71_000881"
     CDS             24102..24737
                     /locus_tag="IVY71_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7984950.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            24873..24965
                     /locus_tag="IVY71_000882"
                     /pseudo
     CDS             24873..24965
                     /locus_tag="IVY71_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            25079..25714
                     /locus_tag="IVY71_000883"
     CDS             25079..25714
                     /locus_tag="IVY71_000883"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7984951.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            25821..27059
                     /locus_tag="IVY71_000884"
     CDS             25821..27059
                     /locus_tag="IVY71_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7984952.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            27286..27705
                     /locus_tag="IVY71_000885"
     CDS             27286..27705
                     /locus_tag="IVY71_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7984953.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            27710..27949
                     /locus_tag="IVY71_000886"
     CDS             27710..27949
                     /locus_tag="IVY71_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7984954.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         8586 a         5561 c         4831 g         9131 t
ORIGIN      
        1 atctttcaat ctctgaatga tagtcttgaa catctgcata atcttcataa atcaaaaaat
       61 caaattgata gtttgtacga tcaattttac gatataaatt catcatgaaa gactctgctc
      121 cacctaaacc cattcgtccc tgaaaatgca aaattctttt cattatattt tcctccatta
      181 actaatatac tcgcttcaaa gcagccatta accctggcat tttcacttca acgaagcttc
      241 caacttttac agcagataaa aatagtggag ctggcatatt gtaaagcaac tttgttattt
      301 tttttgctga agaatccgtc ttagcaattt gcttggcttc tcggatcact ggatcactca
      361 atatctgctt tgcttcgtct ttcttctctt ttgaagttct tgcagaggta ctataaatag
      421 aaaaaataac tgaagttgtc ccttttaaaa gaagcgccgc cataccgtct ttattaatat
      481 cgtctgttac agtatttgct aatgaataga attctttaaa tgtttcgaac ttgttaggtc
      541 ggtattgatt agtgatcccc ttcccacctt caaattcata aatatattct acttttgggt
      601 tcatgatcac ttgatgacag tattcataat aatgtaagtt aaatattcga tcctcacaac
      661 tattataagt tttaaataga atttggttat ttttcagaat atcgctacga tagcatttgt
      721 tccatacaac atacatcaac tgttcattca ttaactgata catgtctttt ataaactctt
      781 cgtgagttag atagtgacat tctgtaacta agttttcttt ttcggaaacg attcttccct
      841 cgtccattgt acgaaaaaca aacgaactaa caacaagatc cgcttttgga ctcttttcta
      901 gcaaatgatc ataatctgac aagatcgttt ttactggcgt gtcatctgca tcgaagaata
      961 agagatattc tccttttgac tgtcttattc cttcgtttct tgctaatccg ggcccttgat
     1021 tggtcgtagt aatcaattca aaacgttcat cattttcaat cagttcttga atgatttcct
     1081 gtgtttgatc tgttgatcca tcatttacta aaacgacttc gtactcttca taatccaatt
     1141 cttttagaat atcaatcgtt cgaccaatag ttgcttctgc attgtatact ggtacgatta
     1201 tgctgaattt cggattgttc atttttacgc ctcccaacta gcgaaagatt ttttcattcg
     1261 gaaaagcaat ttctgccaat gagatcacat tttcgtcttc ttcaagtcct tgagaggaca
     1321 ttttatcaaa taggatcttg atggtttgca gcaagatctt taaatcaagc atcaatgaat
     1381 attttttgat ataaagtaaa tcaaaacgta atttactgtt gtaatcagag acatattttc
     1441 catagacttg tgcataccca gtgataccag cacgaacatt atgccgtaag tagtaataag
     1501 ggttttctgc attgaactga tcgacgaaga aaggacgttc aggacgtggt cctacgatcg
     1561 acatatcgcc tttcagcaca ttgaagattt gtggtaattc atcaaagcgg acagctcgaa
     1621 taaatttacc tacaggcgtt actcgtgcat cattgctctt agctaaaact ggtccagatt
     1681 ttgcttcggc tgtggcggtc atcgagcgga atttatagat tgaaaattcc ttttgatttt
     1741 ttgtgatccg cacttgttta tagatgattg gtcccggtga tgtcactttg actaaaattg
     1801 ctgtgaataa cataaatggt gaagcaataa ctaataaaac aagtgaaacg atgatatcaa
     1861 acaaacgttt gaatagttca tattcaggtt ggattctaaa tccagatgct tcaataatac
     1921 tttcatcttc gaaattcata atatttggat tgatcaatgt caagttttca aatgttgtat
     1981 tcaaaaacaa ttttttttca tttttagtaa ctaattgata gattttgatt ttttcagttt
     2041 cttcgatatg agaagcaaga tagacaatat cgatgtcttt aatcatattt ttgatgtttg
     2101 cataatagtt atcaagaatg actgcttcga ctttatgttt gttgtttttc tttgaaccaa
     2161 aattttggat tgctggtgcg acatcctttt ctaacccaat aatgacgacc tttttatctc
     2221 gacttacgcg taaataaagt ttatatacga gaatacgata gatacttaag aaaagcgtac
     2281 cgagaacaaa tgaaagtaag ataacaagac gtggaaaagc aaaccagcgg cccgcaaaag
     2341 tcactaccat gatccctagt gtcatcaata cctgaccgat gactgtgaca aacacgatgt
     2401 cactgatcat tcgattataa aaaacatatg cacccaacaa aatgttcaac gcaataaaaa
     2461 tcactgaaat aataattgct gagtgttgga aagtttctaa atttcgtaat gggatctctc
     2521 cacgaaactt cagtaagaaa ctactatata ttgctgcatt gtatacaata acatccatta
     2581 atataatgaa tatccgtcgt gcagcactcc attctccatt tttattcata tatacactcc
     2641 taatcacaaa ataatatctc tattatctta ccatagtcac ctttaaaaac ctagtttata
     2701 agcaaattgt aatggaattt aaaaatgtct aataaaaagc tttatacacc tctaatcaag
     2761 acaaggactc taaattacaa atctccagat gaatcgattt tcactaatgt tggtaaatta
     2821 aagaagtcga agtatttgct gcttttttta tccactcata aagtgaacct ttatattttt
     2881 cagagaatcc atcttcccaa agcgctgtct agtccataag aatacctgta ttacgtgaag
     2941 aatcgaaaaa attgaaatcg atttttaatg acattatgct tatagaagaa agtgaggtaa
     3001 tatcttatga ataaacaaga gttgcatgat tttattggtt ttcgacaacc aaatatatgt
     3061 caaattgtcg catataagga tggggtagaa gtttatacag atgaatggaa tggttataaa
     3121 aaagacgata cttgtcatat catgtcagca acgaaaagta ttgtatcttt gctgattggc
     3181 attgctttag agcaaggatt aatcaaaagt atcgatgata aagtattaga ttattttcca
     3241 gagtataaag tgaaacgcgg ggagaaaact atttacgcta ttacaatcaa gcacttgcta
     3301 acaatgaaag caccctacaa atgtaaaggc gatccatgga caaaagtctg ttcaagtgaa
     3361 gattggacaa aaacatcact agatttattg ggaggaagaa aaggtataac cggcgaattc
     3421 aactatcaaa ctgtctgttt acacatatta actggcttgt tgtatcagac aagtaagcta
     3481 acaactgttg attttgccaa taagtatcta tttgatccta tagggatacc aaaccatgtc
     3541 aattattatg ctgaaacagc tgaagaacat aagcagttta caatggataa gtctcctaaa
     3601 accaatgtct ggttttgtga tccactaaat atcgggacgg caggatatgg tttgtgttta
     3661 tcggcaagtg atctggcaaa aattggtgta ttatgcctaa ataaagggat gtataaagga
     3721 aaacaaatcg taccacccat gtggataaac gagatgttca aacctagaac cagtgcttta
     3781 gaagacttac aaaatatgag atatggctat ttatggtggg tattagattc tgagaacgaa
     3841 atatattcag caattggtaa tagtggaaat gttctttata ttgacgctaa gaacaatctg
     3901 gttatctcca tcacttctta ttttaaaccc atgataattg atagagtggc ttttgtagag
     3961 gaatacctta aaccatttat tttaaaatag cctatattaa aataaaatat cgattctttg
     4021 acagctaggg ttcgtgaaac tttggctatg attttgtatc tattggcgct ctgagcatga
     4081 ataagagcgt gggacaaaag taagaaacac ttttgtctca ttctttaaaa acgaataaac
     4141 ggtgggagca gaagcaactt cttcttaaat aagccgaaat tcacaaaaat ttgaaaagca
     4201 attttcgtga atttcttctt atttctcgga gttaaacact tctgtcccaa cctcgactta
     4261 tttttttgag tcggcacctt ctgccttttt ttgacaaaaa actataaaaa gccgttatgt
     4321 gcgagcgaat cacaacataa cagcttttta ttatagttta atctggtctc aacgaaaagt
     4381 cgccgattcc ttcttttgtt aaattatcat tgtaaaatgg atcatgcgca atatatgctg
     4441 gccatttatc ttgcattttt ttgatttcac cagcaaaacg tctttgtttt tctggagtat
     4501 cttcataacc tcttgattta gattcgaaat ggtacaattc tgcttgatgt gcatagacat
     4561 tgtaacgacc cagttcataa actttcaagc acaagtcaac atcgttgaat gctacttcta
     4621 atgtttcatc aaacccgtta acagcattga agtcagctgc cttgaccatc atacaagctg
     4681 cagtgactgc taagtaattg acgtcaatga ccaatcggcc gaaataacca caatgtgttc
     4741 gatcatagtt attcaatgca tgccctgcaa caccaccgat tcctaataat actcctgcat
     4801 gctgcgttgt atcatctgga taaaacagtt ttgctcctac acagccgatt cgatcaaact
     4861 gtgcataaga aaccatcacc gtcatccaat ctggctcaat cacttctgta tcgttattca
     4921 agaataaaaa gtatttgccg tttgcttttt ctgctgctaa attgttaatt cgagaataat
     4981 tgaatggaat gtcaatcaat tctacgataa agcggtcttt cagctgatgc ttgtattctg
     5041 caaataattc ttgcattttt ggatctgtac ttccgttatc cgcaataatg atttcataat
     5101 ttgaataaga cgttttttcg atgatcgaat ccacacaagt ttttagatct tcatagccat
     5161 tcttcgttgg aatgatcaca ctgaccaaat cctcttgcaa gacgtcataa gcaatctcat
     5221 aaaatccaga aattctgcca ggacgtacgc ttcctttgat gtttcttcta gacagggcat
     5281 ctgtcaatgc tttcactccg gaatcataga tataactttt ggcttcgcca ttacttgcag
     5341 ttgatcctgg gatcgtccgc caatgataca agactcggtc aatgtggtaa atatgatctg
     5401 cagggatttg ttctgtcaca cgaaggacca aatcataatc ttgagaacct tcatagcctt
     5461 tacggaaacc gccaagttct ttaacaatgc ttgttcgata tacgcctaag tgagagatat
     5521 aattatttcc catcaatgta tcaggcgacc agtctgcttt gaaatgcgga tcaaagcgat
     5581 tgccatctgc atcgatttta tcttcgtcac tgtagatcac atccagttct ggatgtacgt
     5641 ttagcacttt tgccacttca tataatgcaa atggcggtaa ttcatcatca ttatctaaga
     5701 gtgctataaa ttctccttct gctatttcca atgcagaatt agttgccaga ctgatgtgtc
     5761 cattttcttt tcggaaaacg actttgattc tatcatcttt tgcttgatag gattccagac
     5821 actttcggat agctggatca gtagaagcat catcactgat acatagttcc caatgatcat
     5881 acgtttgatc cagtaccgaa tcaatgcatt tttcgagcca tttgatttca acgttataga
     5941 caggcataag tatggaaatc aatggacggt acgcaaatcc ttgtgcttct ttcctttgct
     6001 ctttcaaatc tggtttctca tgacgactga tccactctgc atagtcgatc gatcctggct
     6061 tcaattccaa tttgacccga cggatcgctt tttttacgcc atgagtcctt gcataattta
     6121 tccctttttt cagtaatctt ttttttcttt cccatgaaga ttcaatgccg tcatcataag
     6181 ggtatttacc gtttaatttc acagctatgc cattttgatg tctcgctgtt tggaagtcta
     6241 agatggcttt tcctctcatt ttccctgata aacgtacctt aaaaccgctt tgggtcttaa
     6301 catcaagatt atataaatga ttgatatcca atcttacgac atgtgttgct tcagcaatca
     6361 cattttcttt ttctaccttt atcgtaggga tttctttggt cacttcatca attgcccagc
     6421 ccacaataag caaatcgcca gtggcccgtt cacgagtgat ttgatcgata aatacggcaa
     6481 tttctttttt ttgcacgatt cgtttactcc ttatctttat aagttaatca ttatttacat
     6541 ttcaatgatc catgattggc cattttgagc tactgcttcc actaagaatg aacgttcttg
     6601 ctcgcaaagg attcgtaact caaatcccgc ttctgttcct tttgcgagct gttctgcttg
     6661 attgacttct tctcgtgaaa caggaattgc ttcgaaaaat ggcgattggt cagctgataa
     6721 cttataggac aagggctggc gatccgaatt agcgattccc catccgatga tttttaatat
     6781 ttttgtttct gcatcccaga ttttctcgtc aatacatata gaaagttctt tttcttggat
     6841 ttttttcgaa gtttctttct tacgtcgtcc tagtctatgt tttattgtcc attcatcact
     6901 ttccaacaat gtgcaatagc gatttgtggt tgtttttaac gtatggtcta gtcttgtatt
     6961 ttcctcaaga agatgatgat tttctctttc taattcgtca tttttctcag aaagctgctt
     7021 tgtgacttca gccatgggac gaatcgtttc caacaattct ttcattgttg gtgtcaattc
     7081 cccgataaag cgatagtgac aatggatagt cacttctttc ccagaaatat ccgttagaac
     7141 gaactctggt aattctttcc catcaaacaa ataacaatca tttactgttt tgactacagc
     7201 attcgaatcg ataaatgtta gtttttctcc ggcagcttct gcgctgaatt cgataaagct
     7261 aggttgttgc gcgaagcgaa acaccattct ttctgttgtt tctgatacgg ggaaacttag
     7321 tgtctcattt tcccctgtga aattattgaa tggtatttgt tggattgttt catcttgttt
     7381 cgtttgctgg gttacttcta acacttttac ataattagaa ttttgtggct ctgcaatgga
     7441 actttgtgct actggatgtt tcatcaatga gaaaaagtat tggtagactt caccaaatgg
     7501 tcgcattttg aaatcatagc gtacagcttc cggcaattct tcataagttg attttaattc
     7561 tgtatcacct acagctaagt acaaataatc ttcaatatta atgaataatc ccgctttttc
     7621 aaaaactttt tggaagccat catgagtata aaaagaatta tgggtttcat ctaggagacc
     7681 ataagaagcc cacggcaatt ggttattgaa caaatcgatc atgacagaat tatgcgccaa
     7741 gttagggaat gtaatcagga tctctccttc ttcattcaaa aattctctga ctttcttcaa
     7801 tgtttttcca ggatctacca aatgttctaa aacatccgca aacaagacgt aatcaaatgt
     7861 ctgtccagca taatagtttg cccactcaaa actttcgata tcaccataaa atccatcttg
     7921 agcaaattcg ctgacaaaat cgaacaattc cttatctaac tcaacaatgc tcacttcaca
     7981 ttgttttgct ccgattaaat gtttcgttaa tcggccgttt ccgggaccaa actctaaaac
     8041 tttacttcct ggtttgatct gtgcggcaat ttttcccaca ctgctttgtt catctaggtc
     8101 catttcaaaa tcatatttca ttgctatgag tcccttcttc ccatttatgg tctaagacca
     8161 cgatgccttc tccgatataa gaaccgctga caaacaagtt catgtacttt gtcttatata
     8221 ccaaaggaac cgttgcattt tcttcaaaga ccgcaacgtc aaaataatat tcaccagcta
     8281 acaaacctaa cttttcatac tccagataaa agatattctc tccttttttc caaggaatct
     8341 tgataccatc tagtagagta ttaagtccgc atacataaaa attgtcgact gttcgtagcg
     8401 caattcctaa aaccggattt ttgattgtat catcttttac ttcataagtt actttaacgt
     8461 atagtttttc tccttgttcc agcatatcca ccggattttt gtctttatcc aacaattccg
     8521 ctttcgtcac gtcgacgata tctggagcgg cgaaccgttc tacttggttt ttattccgat
     8581 ccactgtttt aactgatttt ttctttaaaa agttttcata attcgtcgtt acttccatcg
     8641 tatcgccata ttcgacgact tctccgtttt tcaaccaata ggaacgatca caaaaacgac
     8701 gaatcgaact gatatcatga gaaacgaata aaatcgtctt tcccgagttc ttgatttctg
     8761 taaatttttc catacatttt agttgaaatt caagatcgcc tactgccaat gcttcatcca
     8821 caatcaaaat atcgggattc acattgattg ctacagcaaa agctagacga acgaacatcc
     8881 cgcttgaata agtttttaca ggttgataaa tatggtctcc gatgtcagca aattcaatga
     8941 tatcatcgac catttcatcg acttcttctc ttgaaaatcc tagaaccatt ccatttaaat
     9001 agatattttc atagccagaa tactcaggat tgaatcctga tcctagttct agcaaagctg
     9061 agatttttcc ttcgatttcc atcgtgccgg atgttggtgt cagtactccc gtaatgattt
     9121 tcaacattgt agattttccc gatccgtttt ctcccacgaa accgatcatc tcaccttttt
     9181 ttatttctac attcacgttt ttcaacgcat aaaatacatc atgataggat ttcttaaaag
     9241 gattgagggc ttcacgaaat cgatccgaag gttttgcgta catattataa gattttgtga
     9301 tatcttttag tctaattgca tattctgtca tagctattct ccttacagca catctgaaaa
     9361 atgtggtttc aatctacgga acactgcaga gccgattacg aacattacta acgtgaaaac
     9421 ccagaaatac aaagtatatg ttccatgctc ccagaaccaa gcttcactta aaaatgactc
     9481 acgataacct tgaacaacat agtacagcgg atttaatttc aatatgctta caagacctga
     9541 atcaaactga ttagggctcc ataatatcgg taatgtccac atgatcaact gcagcacgat
     9601 cgtaattaat tgcatgatat caggtaaaaa tggttggatc gatgccgtca gccaagtcaa
     9661 tcctgtcaag aaagcacaca tgcaaaataa ataataaatc agctgtaaag acattgctga
     9721 aggatatata ccatttataa gcgcgacagc tatgccaatc agaatgaaga ataagtgggt
     9781 gtacagattg gataggattt tcgtcgtggg caagatacga atattgaata cgactttctt
     9841 tactaaataa gtatattccc gaaaaacaac cgtagttgat gcaaatgcat ctgagaagaa
     9901 gaaccaggga atcatcccgg taactaaata tacgataaat gggtattttg catcgatcat
     9961 ttgtgctttc agtccaacac caaataccag ccaataaatc aatacagtaa atagcggatt
    10021 gataaacgcc cataagattc ctaaaaacga tcctgcatat cttgatttga agtcattgat
    10081 cgaaaactgt agcaataatt ttctgttctg ataaatgtcc ttcataaata aatacaaatc
    10141 tttaaacatt tgtgttcctc caagcgattt tcagtgaatt acagtcaagt gccattttac
    10201 catatttttg gtaccgagct gcgttcttat ttctttctta attttctatg atacaagatt
    10261 ttctctaacg tttctatttt acagaagatg ctagacaatt gcaataaacc aaaaaaggct
    10321 gcgacggttg tcacagcctc tctcgtattt tgtgcctatc aatgatagcg ttggatcctg
    10381 gaagaatgag cgctatctgg ttgttcctca taagatgtat catcataagt catatcgtca
    10441 tatgcctctt ccataacata gaattcctcc gcaacttctg gttcatcata tcgatgaacg
    10501 gccatagcca acaggatata aataatcgga acccatgggt atgaagtcaa tgtatcctcc
    10561 ataaatgact gcatcatcac cgcaaaaaag gcccagataa aagtcatctg cccgttggat
    10621 aaatctgctt tcaacgttaa gcgaccaata aaaaggataa gcagcaaagc agcaatccca
    10681 aatcctatca atccatagat attcaaaata gaattataac tattgtggtt tcctaaaaat
    10741 tggtttcctc gttggaagat aaatgttttc attcctagta aaatttgttc agatttttct
    10801 cctagcgtct gatagaattt atgccaaata tcttctcgac cggtaaacaa attgactttt
    10861 tcagagacag ccgccagata agaaatcggc aaagctaaaa gaatcgtaac cgtgtaagca
    10921 ataaatggcg cacgaatgac acgcatcaac gatttaggta aaagatccaa tatcgcaaaa
    10981 acaatcaacg cgactaatgc acctctagat tggcagaccc aaatcccgag aaaagctgca
    11041 acaactgcta ctgcaccgat gattcgtaca taccatcgtt caaaagaact ggcaaatcca
    11101 ctgatcaata aagcaagagt catcaaagaa cttccgatta tattcgtatt gatccaaata
    11161 tcagaaagcg aattgcctgg agcccagatg ctttgtcctt ctggtacttg gatctctgta
    11221 aatacacgat ataacgtgat tccgaggaag accgaaaaga caatcagata aataccgcga
    11281 tcccattttg tccactcgat ccatttaatt actaaacaaa ataaaataat caaaggaaac
    11341 agtaagtctc caaataaaga ctcatttctt aattcccgaa agtaagaaaa gaaaatgaaa
    11401 agggcaagcg caacacctag ataaagaaaa tcgacgcctt ttgctttata aacatttaaa
    11461 atgagcaaaa tagccacact aaaagcgata agtaatgaac tattgttata tataaacaaa
    11521 gttgctggaa aacctaaccc tgctgtccaa acaaaaaaga ctagcgtcag taacaaaagc
    11581 aagaaaccga cactcatttt gttgacaaat ttttgtacga ttgcttgaat tatctccact
    11641 ctcctttcta ctaacatttc aggattattc tcagctagcc tattactgta cgactagctg
    11701 attttcctga gtttgtgttt gtggtacatc tggataatcc ggatattcta ctttaaaaat
    11761 catataaccg tctttatccg gtccatatag ctgcgtgaac tgcgcttcat ataattggtt
    11821 gtaatcagtt aatggtcgtt tactgatcac atagtcgatc cctaattttt ctgcgtcttc
    11881 aaaattcaac gtgaccgaaa acatatctgg acgatctaaa atgaactttg tatcttcctt
    11941 agcgataaaa gctcttgtat gagcataccg attataatac ttctcgtatt cttctttcgg
    12001 atcgattttc ttccatgcag ctatatctgg ataaaagcgt actgtattga aggatttcac
    12061 tcccaatgtt ggggtgaagt tatataattc atcttcagac aaccaaactt taccaggatc
    12121 ttgttgatcg attttttgga tttcttttgc cagtgttttt tcaaaaatcg ctcctgtacc
    12181 ttctacgact ggattgacga aaaagccaga tacgataact aaggcggcaa aagcgaggta
    12241 aaacaagcgt ctccagccaa ataatgcaaa agctaaaatc actgttccca acacagcaac
    12301 tgcgatggtt tcaaaatccg aaaaataatt atccattttc gaatgcgtaa tcgcatagaa
    12361 ataagaccat gcatttactg ccaacaaaat gaatttatac caaataggaa taacatcttc
    12421 tctccaaata taagcgataa accagatcga taataaacaa gccgcaaaac taaaagtaag
    12481 gatggctctt aatggaggaa cataagacag cagagtgatt tctgctagcc attttggcaa
    12541 cccaacggta atccaaaaga tagaaaacag acaaaaaatc atcaaaatac gtcccagtaa
    12601 cttttgttcg ctatcttttt tgccaaataa cacaaatgga cttgctagga atacagttgg
    12661 aaaaaagtga tagaacaggg ccacttcaga gttattcgaa tagttgatat cttcaaaagg
    12721 aattttccaa tttgtaagga aagaaaaaaa ttcgccaatc gtccactttc ctccagtact
    12781 tacccgtttt cctggatata aggtattcaa agaagctaac aatgcgtctt ttgacgttag
    12841 ccagaaatga accatgactc cgccaataaa taaaactgcc cctccaatca acactacatc
    12901 caacagatcc catgaaactt tcttgctata gtaaataagc aggccaacaa aataaaaaat
    12961 cagcaagtac gccagcatca cttgatgggc tgggtaaatc actaaaataa acccaatacc
    13021 gaaaataacc accaaaagca tcatcaacgt tcgtagccat tttttatcat gattgtagaa
    13081 ataatgataa aaagcagtca tcagtcccaa agtaaagaaa atcaaatcgc ccacatgttg
    13141 catgaaccac cactggactg ctggagaaaa tgtcagcgca aatgcaccga ttagcgatag
    13201 agcttttttt ctcttcgtta atatcatcag tacttcgaat ccaagcaaga ccatgacgac
    13261 taatttcatc gcccaataaa aggaaagtcc tcgatctctt ccgaggaaaa agaatcccca
    13321 gttgaatggt tttccgataa ccgtaatatc ctttacagga gaattatacg caatgatcat
    13381 gttttgtccg tttaatgaat aatcctcgtt caccaacgga aagtcctctt cagcttgtga
    13441 gaggtaaaaa ggtgtctgca ccagccattc gtctgaccgc acttcccgat tctgtccaaa
    13501 aatcacatca gtttttttcc catcgtctct ttgcgacaca tatttatccc aaacaccaag
    13561 tgaacttccg tttaaattaa aagtaacgat gaccacgatg actaaaacgc caataagata
    13621 gcggagtttt agaaacaggt caagcaccaa attcgaatct gcctggttcg cttttggctt
    13681 ttcttttcgt cgtgcacttc ttgtttgttt ttccatatca tgctccaaat tctatttgat
    13741 aaaaggaaat tacttattca tttttttggc agcttccagc atttgcgcaa gtgcttcttg
    13801 ccaagttggt atgacaaatc ctaactcttc tgtctttttc aggctcatga ctgaatactg
    13861 cggacgtgtt gctttttgcg gaaattcttc tgacgtaaca ggtgcaactt ctacatctgt
    13921 atctttcaat atttcttttg cgaattcata ccaagaacag ctattttcgt tcgacaaatg
    13981 ataaatacca aatggtgctt tttcttcgat gacatatacc ataaattctg ccaaagtacg
    14041 tgtccaagtt ggtcgcccaa actggtcatt tactacagtc agtttgtcgc gggtttccgc
    14101 taggcgttgc atcgtgaaga cgaaattgtg tccatagatc ccgaaaaccc aagaagttcg
    14161 tatgatataa tagtcttcta aaatctcttc tactgctttt tcgcctaata atttcgtacg
    14221 accatattca tttagtggat tcgtttggtc gtctacttcg tattctacat ttttgttttt
    14281 tccatcgaaa acatagtctg tactgatata gacgaattga gcattcacag cttttgctgc
    14341 aagtgctaca tttcttgtac catcaacatt gatcttctca tctaattctt ttgcttcttc
    14401 ttctgcttta tctactgctg tgtaagctgc acaatggaaa ataacatcag gttttaattc
    14461 tgtgatgtaa gccattgttt tctcggcatc tgtgatatcc aagtcttttg catctgttga
    14521 aacatattgc agattttttt catctaataa atggcgcaat tctgtaccta gttgcccatt
    14581 tcctccagtt aataaaaaca tatgattatc cgctccttga tttttatttt aaaaaagaga
    14641 caggcgagcg cctgcctcac atgtgttttt ccattaatac agtgacattt tctctgtatt
    14701 attgtccatt tttggcatat gctgcttcaa cggcctcttt ttcttttttc caccagtctt
    14761 catgttcttc gtaccatttg atcgtgtcag ccaacccatc acggaagttc gtaaactcag
    14821 gcttccatcc taactcttcc cgtaattttg tcgaatcgat tgcataacgc aagtcgtgtc
    14881 ctgcacggtc gtttacatgg tcgtacgcat cttttggttg acccatcatt tctaagatca
    14941 attccatcac ggtcttatta tcttcttcac cgtctgcacc gatcagatag gtttcgccaa
    15001 ttctgccttt cgtcaaaatc agccacactg ctgaagaatg atcattcgta tggatccagt
    15061 cacgaacatt ctttccttcg ccatataatt ttggacgaat accgcttaag atattcgtta
    15121 cttggcgagg aataaatttt tcaatatgct gataaggacc atagttgttt gaacagtttg
    15181 aaatggttgc acgtaagcca aaagaacgca cccatgcttt tactaacaga tctgaaccag
    15241 cttttgtaga agaatatgga cttgaaggat tatatggtgt ttccgcagtg aatttttctc
    15301 cttcgccttc cccatgtcct ggcaggtctt cacgtaatgg aagatcgccg taaacctcgt
    15361 cagtagatac gtgatgatag cgtacatcat acttgcggca agcttctaaa agtgtatacg
    15421 taccaatgat attcgtttgt acaaatggga aaggatcttt taatgaatta tcgttatggg
    15481 attctgccgc gtaatgaaca actgcgtccg cttcttttac tagacggtcc actaattctg
    15541 catctgcaac atctcctacg accaattcta cgcgatcgct aggaagtcca gccaagttct
    15601 ctttgtttcc tgcataagtc agtttatcca aaacagtgac atgcacttct ggatgattgt
    15661 ttaccacgta atgaacgaaa ttagagccga tgaatccggc acctccggta acaatgatgt
    15721 ttttcataag aaaatttctc cttaattctt agatttctcc gtaaacaaat ggattttctg
    15781 cttcaaattc ttttaatgtc ggatgacggt gatctttttc cgacataatc gctcttgatg
    15841 gatcaattgg ccaatcaatc gctaaatctg gatcatcaaa tgcgatgccc ccatctgctt
    15901 ctgcattata gtaattatca cacttataaa ggaagttcac atttggtgtc aaagtaacga
    15961 atccatgggc atatccttta ggtacaagca actgtcgatg attatgttca gataagataa
    16021 atccgtccca ttgaccataa gtcgggctac ctttgcggat atccactact acatctaaaa
    16081 ctgctccagt gactacccga atcaattttg tctgtgcagc ttctcctttt tggaaatgaa
    16141 gcccccgaag gacaccagct tcagctgata gtgaatggtt gtcttgaaca aagtcatatt
    16201 tcaatccatg ttctgcaaat tttgcttttg aataactttc tgtgaagaaa ccgcgatgat
    16261 cgccaaagac gtccatttct acgattttga catctgttaa ttttgtatct gttactttca
    16321 tctgtaagga ctccttattc agcggccaaa ttcaataaat attgaccgta ttgatttttt
    16381 ttcaatggct gtgcaagggc cagcaattgt tccttatcga tgtagcccat acggtaagca
    16441 atttcttcca aacaagccac ttttaagttc tgacgttttt caattgtttc gataaaggta
    16501 gatgcttcaa gcaatgattc atgtgttcct gtatcaagcc aagcaaatcc gcgtcccata
    16561 agttctacgg atagttttcc tttatccaaa tatgctttat tgacatccgt aatctctagt
    16621 tctccacgtt ctgaaggttt gataccttta gcgatagaaa ttacctcatt gtcataaaaa
    16681 taaaggccag ttacagcgta atttgatttt ggctgagcag gcttttcttc aatcgacaaa
    16741 gcttgcatat tctcatcaaa ttctactaca ccaaagcgtt ctggatcatt gacatgatag
    16801 ccaaatactg tcgcgcctgt atcttttgaa gctgctcgtt gcaacatttt agacagaccg
    16861 ccaccatagt aaatattgtc ccctaatacc aagcagacac tatcatctcc aataaattct
    16921 tctccaatga taaacgcttg tgccaatcca tctggacttt cttgtactgc atattcgatc
    16981 tgcagtccta aatcatttcc gtttccaaat aattccttga aacgtggtgt atcttgtggc
    17041 gttgaaataa tcagaatttc tttgattccc gccaacatca gagtagacat tggataataa
    17101 atcattggtt tgtcataaat cggcatcaat tgtttcgatg ttgcctttgt taaagggtac
    17161 agacgtgttc cgctgccccc agcaagaatg attcccttca tggatatctg ctctcctttt
    17221 tgatttcaaa tttcactttg ttattatctc atcaattgtt atatttgtca tggaaatatt
    17281 aaggatttaa acatctttta tcaacgttta aaccattttc gtttgatcaa cgccgtaatc
    17341 agatgaacac gccaagaaat tttttgccgt aatttagaat gtgtttcaat ctcactcgcg
    17401 tttaaatcat gtcttcggta cagagttaac ggttctctta ggaacccagt cttctttcct
    17461 cccagcaaac caatccacat atcatgcatc ggcacttctg acgggatcgg caaaatatcg
    17521 ggttttaaag aagaccggaa agccatacca gcaccaatat agtgacttct tatcacatta
    17581 ttaagccatc cttctttgga atttcgaaac gaaaaatacg aatcatggat ctcttgcagc
    17641 tcgctgtcca caatgattaa atcagaaact actactagct gatccggatt ctgctcaaaa
    17701 tagtcagtga tctttttcac ttttgtctcc aaccaaacat catcttggtc tgccaaaaag
    17761 attagatctc ctttcgtctg ctggatcgca taaccaaaat tagcgatcaa tccttgacga
    17821 ggccccgaaa aaagctggat tcgcttatct ttttctgcgt attcagttaa tatatcccat
    17881 gtatcatcta ctgacccatc atcagaaatg actacttcat cttctgctga aagctgagat
    17941 aaaatactat ctaactgctc tcttaaatat ctgcctccat taaaggtagc tatacaaacc
    18001 gaacgcacga attcacacct tccaattctt aacatatgag cgcaaagtca tccgccagat
    18061 atgccggttt ttgacggtca agaactgttt agctgttctt aacaataatt gtcgtcggtg
    18121 actggataaa tgcctttgat catattcatg ataaaatcga ttttctgctt gtaaaataga
    18181 acggtatcga ttacttgaaa ctttttcata gtctaaaacc gaaagatcat gttccattcg
    18241 tacaggaagg atctccacag ttttttccaa ctgatccact ttccaaaaca gccaatgatc
    18301 caaaaaatct aaagaaaaag caggattaaa cccgccaatc ttttttagaa atttgacaga
    18361 ccaggcaaca cctgaattga ttgccatgat tcgttcagct gttgggcctt caacttctac
    18421 tgcttgagct gtacgattga tataacgatc tgaatatagg ggagaaactt gtcggctccc
    18481 agaaaaaatc atcggaactg cagcaacaca ttcatctgta aaggttactt tacgcaatag
    18541 ttcaaaataa tcgtcgacta gcctcgtatc ctgatccaga gtgacgagac aacgtgttcc
    18601 ttcatctgct tgagacaacg caaaattata agccaccgct aaaccaggat tttgcggatc
    18661 atgatgataa acgatatttt ctttttcaaa cagctcattt tgctgccttt gaggactatt
    18721 atcgtaaata ataagctgaa tttgcttttc gctaagtgct ttgcttaatg gtccataagc
    18781 tggagattga ctgaatagtt gctgataaag cacaacgact actaatattt ctggtttcat
    18841 cagaaaaaac gccaccccca cttattgaaa aatttcatca tagaagtcaa aaacacgcga
    18901 aacagttttg catttcgatg cgcacccttc ccgtaaaaat ggataactgc ataaaagggt
    18961 gtgtacaata acttgtaacc tgctttttca aaactcaaac acaagtcgtt gtcttcaaag
    19021 tacatgaaga aacgctcatc aaaccccttt atctctttaa atttatcaat atcagctacc
    19081 ataaaactac ctgagatcat ccgtacataa ctattagcat cagtaggcaa tcctcggcat
    19141 tcaaacacag ccagtcgttt gtcaaacttt tttttgaccc atttgaaagg cacaaagcgc
    19201 aaaacgtaat caaaaacagc cagctgctca cggatgaggt gctgagttgt tccatcttca
    19261 ttaagaatct taggtgctaa catcgcacat tccggatgtt ctttcaataa caagaccaat
    19321 gctgtaactg tctcttcatc cactaaaata tctgggttac aaataatgcc atagcgagaa
    19381 tcgctagcta tcagattgtg attatgtcca taaccaaatc cggaattttc atcatgaaaa
    19441 tagatatcga tgtagggata tttcctatac tctgttagtc tttttttgta ctcgggaagt
    19501 gaattgttgt caaaaattcg gatttttact gctgtttcat cttttacagt tgaaataaaa
    19561 ttatctagtg tccgaaaaat tttatcactg tttgccgtca caatactaat aaggatcggc
    19621 tggtttaatt tgttattcat ctatatgacg gttccatttc ttcaaatatt tctgttttac
    19681 ttgcgtacgg tactctttat tccctaaaaa tcgcaatgtt tttaaaataa actgatgttt
    19741 ctcattgatc agaccaatca attcaaataa taaaattgca gcaaacaaca tggcaataat
    19801 caatatgatc gttccgattc ttccagtata attcatgacc atagctgtga aagaaaacat
    19861 caaagccatc gcataaatcg tcaggacagc acctttatga gtaaagccta aactgattag
    19921 tcggtgatga agatgttttt tatctgctgt agaaaaagaa acacggtttg cttttctcct
    19981 aatgatagca aaaaacgtgt ctgttaccgg cactcccatg acgatcagca aagaaatcga
    20041 tgaaacaaaa gtcacgttct tcagcccttg tagtgaaagg acggccatca taaaccctag
    20101 gaatagagct cctgtatcgc ctaaataaat ctttgccgga taaaaattgt aaggaaaaaa
    20161 accgataata cttgctacaa gacagaaaat aacaactgga atatacactg tagaagcatg
    20221 gaggaaaaaa tatccaatga ttcctatcgt aattaaaccg atcaatgaaa tccctgccgc
    20281 cagcccatcc aaaccgtcaa tcagattgac cgcattcgtg ataccgaaga tccaaaaaat
    20341 cgttacggga aaactaagcc agcctagttg gatatatcca ataaaaggaa gcgtgaagga
    20401 atcaatacgt atatctgcta caaaataaat aataagagaa gcaatcaaga tccctatact
    20461 tttttgttta ggtgtcagct catatttatc atcgagcact cctgtgacta tgattactaa
    20521 agaagcaatc aagatcggta caatatacga ttgagggata attttacgga ataagaataa
    20581 agtcgatata acaaatgaaa taaagatagg cagtccgccc gctgaaggca ttggaacttt
    20641 atttattctt ctttcatttg gtttgtcgac catacctgtt tgtactgaaa tgattttgaa
    20701 tacaggtgtt aatatcagtg ataataaaat tgtcagaaaa aatcttataa ccatactaaa
    20761 agaaacaagc atatgggcac ctactttaat taagagtcat aattattata aattatatat
    20821 gtctttttga ctatcttttt atatcttctc aaatgtccgc aaagccttat tttatcggct
    20881 ctacgggcat tttgcttttg tggtaaacct cgcatatcta ggtctatctt cttatatttt
    20941 ccctgtcaat cgtggttaaa atcgtggtaa atacttctat gcgattagac gctgaacctc
    21001 tgattttgcg gtatctatgg acgcatgagc gtaccagttc attgtgatac tgatgtttga
    21061 atgccccatg atatactgta aatctttcgg gttcatgttc ttgcttgcca gccttgtgca
    21121 gaatgtatgg cgtagcgtat gcggtgtaat atgtggcaag ggattgtcct tgtggtgctt
    21181 gttgtatttc tttaccatac ggataaataa ggcgttataa tcaatggcaa cttttggatt
    21241 acctttcgga ttgataaaca caaaaccgct gtgtccgtct acctctatcg actttatctt
    21301 tggtcggctg ttgataaccc gttgaaatgc ctgtatcgtt tctttgctta atggcacttg
    21361 ccttatcccg cttttcgtct taggcgtttc aatataatag ccctgttcct tactctttag
    21421 taactggtgg tcgataatca caacttcatt cttgaaatca atgtcggcta ctgtcagtcc
    21481 gcacagttcc gagatacgaa gtcctgtctt taacagtatc agcacatcat cataatgctt
    21541 gtgatacacg ttgtccgtct taataaatga caataaggct tgctcctgtt cctctgtcaa
    21601 tgcgactttc tctttggtat cattttctat gacttcactt agcttgaaat caaaagggtt
    21661 tttcctcaca taatcatctt gtatggctat gtagaatgac gcttttaacg agcgtttatg
    21721 gttgttaatg gtattgtagg aaaagccttt gtctttcatg cgtaacgccc attctttagc
    21781 gtcagagggt tttatcgtat caatgctcct agcacctaac ttgtcctctt ttaataaccg
    21841 catgagttgt tcccgttgtt tctgtgtgct tttcttcaca tttgtcctct gtgcgttctg
    21901 tttggcatag agttggcaaa gcgtcatttt cttgcctgtg ctgtcgatac cgtcctcaat
    21961 atcccgtctt atctgctgtt ccagttctcg aagtgagggt ttttcccgtt ttccctttgg
    22021 tgtcgggtct gtgggtgtca atctccaagc atacacatat tttgtgtttc ccaatgcgtc
    22081 tacatattta tataagtatt ttccgtctgt tcgttggctc tctccagtat gcaggatacg
    22141 acctttgtta tcccgtcttt tttctttcat ggtgtctgct cctttccttg ttggaaagag
    22201 ccttgatatg acttgtgttc atcataacac atacaaggct catttgcatt agattgcgtc
    22261 caatttatca ataacctttt caaactgtcg gcgtttaatc tgtatgcggt tgccattcat
    22321 aatgagccag ccagcgtcct tgttttcctc tgccaatcgt cttaacttgt tttctccaat
    22381 gcggaaatac tttgacgctt cttcaatggt aagggtgtat ttttcccata cgggaatatc
    22441 ctgtgtatta ctcataaaac accccctcac tttcgattac gtctgatatg tccgtacaca
    22501 tctgttccac aagcatttcc tgtttttcag aaaggtgggt acattcatca atcattttca
    22561 tgtagtttga acctgtcagc ctgtcgattt cctctatcat cacgattgac ggcatgacct
    22621 gtctttccag ccattgcaga gtgcgttcca tattgacagg tacagggttc atttcaaaag
    22681 gtaaactgtc atgggaaatg aatatctccc acaatggata gtcaggaaat tccacaaaat
    22741 cggtaatgag ataaaacacc agcccatgag ggttatacgt caataacagt tcttcaactg
    22801 cctgtaccgc ctttttgtca cgcagacgaa tttcaaaacg gttgataata tcggtatgtt
    22861 tctttttcgt tgcctgttcc ttttgttttt catacaggca gaaatatttt gtgcttgcct
    22921 ttgagccgat ataaagggta cttgctaaat tacggttttt tccgcttaat tttccacctt
    22981 gtacattttc atagttttta cagcgacatt ccgcaccacc atttctgtat ttttcagata
    23041 gcacaggaat atccagcaga ccgcacatat cattgattgc caagtcaaaa cgtcggataa
    23101 caccgccaca gtcaagacag cggtttaaga atgaatacca gtccctgttc tgtgcctgta
    23161 agacatattc catgttgcgt gagcctgccc cttttaattc taaaaatacg cccatgtgtt
    23221 catcatcaga agccatgacc ttgatttccc cgtaagcata atgctcctta tagccataat
    23281 acccatagtc ataatggata aaataatctg ctttcatgcc aagcaccttt cggataactt
    23341 ccaatgcgtc agtcgttgga aaacggacag acagataatc aacgagcaga taaaaaggct
    23401 tatcgcagat atagtaattc agacaacgca ggataatgtt ctgtgtttcc ttgctggcgt
    23461 tagcttttcc gttctcaaag cggttgatac tatatcttga tacatgggta agacttgcaa
    23521 gttctccttg tgaaagaccg ctggcaagac gtttgttctt catttcaagt gcaaattcta
    23581 aattgttcat tgtattcctc caatcccaca atagccttga aatacctgta aaccaccttt
    23641 caaaaagtgt gatatgtact atcttcttaa aacctctcat tactactcac aggtttgggg
    23701 gtgtatggtt tttgacttgt cttttgtgaa atagtgcccc cctgttagat aacgggggca
    23761 cagacaaggc tcgcaggctc gccagccgac aaagcatttc ttccccttga cacaaggtgt
    23821 catggcggta agcctgagag gcttccgcca ccgtgtcaga aatactttgt cagcaagttc
    23881 tgttttgcct tttctaacaa gttccatttc aaccgatatt catatctacg gatttaatca
    23941 gataggtatt tctttttgaa tgtttttata tttcattcta ttgtgcttat tcgattacaa
    24001 gaacatagta attcttatat gcttatttgt caatatcttt ttttgccttt acaaatcata
    24061 ttaagcatga tagacttata atagcgaaga tataacgaga tatggagggt tatgcaatga
    24121 aaataagcca tttaggaaat aatatacaga ccataagaaa atttagggga atgaaacaac
    24181 aggaacttgc ggacaaaatc ggtatcaata tgcagagcct ttccaagatt gaaagagggt
    24241 tgaactatcc tgcctatgaa acgctggaaa agataatgga agtgctggac gttacgccca
    24301 atgaattgtt gtcgggagaa tggaagtatg tcaatcaatc tgaaaaggaa gtctgtcagt
    24361 ttttaagagc cgaagaacgt ctaaatgcag aactgaaaca tggacactat gataacttct
    24421 ttgacagcga ggaagaatgg ctggaatatg agttagaaaa gttacgggaa tatattacag
    24481 actatatcaa cgggaaaagc attgaagcgt ccgaccttta cccaatcaaa gaatttatcc
    24541 agcatttgaa gtttcaaaaa ttattagacc gctatgatga tttatacagt atggatatgt
    24601 ttggggaaag catagaggga cataagtata agacacctta tcaagtcgtg aaaatgataa
    24661 acccgaactc aaaagaagat atggaactgt tacgagaagt attaaggaat aacaattttg
    24721 atgatgagga cgagtaactt ttttcctctt gcgagaagat acagcaaaca aaaagcccga
    24781 tcaagagtgc aaagcaccac caatggtggc aaagctcttg acagggcttt ttcttttctg
    24841 ttgtgggcaa tcaagaggaa gaaagggata aaatctgatt tgtggtaaat caggcaaagg
    24901 caacgcaggt atcacagtat gaattgacac cgcataagga aagcaactgg aagattgtag
    24961 aataaatgaa aaaggcttgc attctgcatt tttataaaat tgcaagtctt tatattgaca
    25021 agcataaaat actaagatat actttaatca gaccaacggt ttgtattgga ggtaagttat
    25081 ggcacgaaat aaatatccag aagaaacagt aaaactcatt ttagatgttt ctacaaaaat
    25141 gttttgtgaa aaaggatatg ataacacatc attgcaagat attatcaatg aaacaaaact
    25201 ttctaaaggt gctatttatc atcatttcga ttcaaaggaa gatattttaa aagcaatttt
    25261 tcaaagaatt ggaaatgaaa atgcagtaat atttgctaaa attagagata acgatagttt
    25321 aaacggttta gaaaagttaa gagaaatatt taagacagcg gtattcaatt ctaaccaaag
    25381 cgttttattg acagtttctc ctcaactatt aaacaatcct cgttttcttg ctatgcaaat
    25441 agaacaaatg tatgaaattg tagtacctga atttgttgaa cccgttttag aacaaggtat
    25501 caaggataag tctttggaga ttgaaaattc acatgaaatt gcagaagtta tcatggtatt
    25561 aagtaatgta tggctcaacc cattagttga aatgacggat aaagagggta tggaaaaacg
    25621 ttgcaataca ttcaatgccc ttttaaatgg aataggcata gacacattat tagacgaaga
    25681 aacaatttct gcatttatac agttttgtaa ataactgtat ttgttatccc tatacgatag
    25741 actgctctat aaaagagcag tttatttaaa ccattatata aaccgacggt ctgtttgatt
    25801 aaataaaaag gagagatttt atgcaagcag aaaaaaaact ttttaatcgt gattttatac
    25861 ttgttgttat aggacagata atttctttat ttggaaacgc tatcctacga tttgcattac
    25921 cgtactattt gttaaaagaa actggttcat cagctttgtt tggtattgct acagcctgct
    25981 cttttttacc tatggttatt ttgtcgctgg taggtggtat tattgctgac aggacaaata
    26041 aaagaaacat tatggtcata ctagattttg taacagcaat cataatttta gtattttatt
    26101 tgtcattggg aaaaattcct atcatacctt tgtttattgt tgctttgatg ttactttatg
    26161 gtatttctgg tgcatatcaa ccagcggtac aagcaagtat tcctctattg gtatcatcag
    26221 ataaacttac cgtcggtaat gctataataa atcaagtaaa tactttagca aactttatcg
    26281 gaccagctat aagcggagta gtttttcaat tttatggtat caatcccatt ttaattgtta
    26341 gtattatatg ttttactttt tcagccatta tggaaatttt tatccatatt ccacaccaaa
    26401 aaagacctcg tgaagtaggt gtaattgcag ttgctaaaaa cgatttaaaa gatagttata
    26461 cctttttgaa aaaggagaaa cccattttta tatccgtaat ctttttggtt tgcattttca
    26521 atttggtttt aagtgctgtt ttaattgttg gtctgcctat cttaatttct gaagttttaa
    26581 aaatgccaga tacactatat ggtttttcac aatcagcatt atcattaggt ggtttatgtg
    26641 gtggtatttt aacagctctt gttgcttcaa agttaaaact aaaaaacctt tataccttgt
    26701 tacttatttg ttctgtttct gttttactta tgggaatttc gctattttta gaattgcctg
    26761 caatagtttc ctactgggta gttacagtga tgtgctttgt ggctatgggt gtatctacta
    26821 tgtttgttgt tcaaatttac actcttgttc agacacaaac accaccgcaa ttagtaggaa
    26881 agataatggc tacgctggta gcagttgcta tgtgcggtca acctattgga caaatggttt
    26941 acggtatttt attcgatgta tttatacaag aaacatgggt tgttatgttt gtagcttcta
    27001 ttgcttcaat catgatttca atatattcaa aaagaatttt tcaaaagtta gagatataat
    27061 acaaggggtt aaatagtatg ttaggctaaa aacaatatat aaaagaaaat ttgaaatctg
    27121 ctaaaaggat agtgttataa aagaaagagt aatattgcag tatttaaggc tcgcacaaat
    27181 atatgttttg tgtgggcttt tttcttacca tttttctgta tttctaaagg gtataaacgg
    27241 tattccgttt atatatttca gcattagcag aaagggggtg agattatgag gacatcttct
    27301 ttcgagcata tcgtcagatt acagttcaat tctttaatgc tgattgtcat taagaacaaa
    27361 gtgaaaagtc gaaacagaca gcttgcaaga cattctaaac gtgaagtatt gttttgtgaa
    27421 atagccgaaa cgaaacaagt tgaacgtgga acgattgata cctgttttta tgattgtgta
    27481 tcatttaaag tcttgcactt tacaatttat gtatctgatg aaacacttgg tacagtttta
    27541 catagactgt cagaaaaaca gcgtagtgcg attttattac gttattttca aggaatgaac
    27601 gacagagaaa tttcagaact gtaccatgta tcacgttctg ctatttgcag tagaagaact
    27661 agaggactaa ggaagttaaa aatgcttttg agcgaaagga agtaaagaaa tgagtaaaga
    27721 ttatggctat ccctcttttg aattgatttg caaagcgtca agcggagatg aaatggcagt
    27781 taaagagatt ttgaagtttt atgacggtta tatttctaaa ttatgcttgc gtccttttta
    27841 ccactctgaa agtggtaaaa tcattatgca ggttgatgaa gaactaaaag gagaaattca
    27901 tacagacatg atgaaagcaa tattgaaatt tgaaatcgga gtgaagtaat caactgtata
    27961 aaaaaatcgg gagatacaca gtttcgtgct atctccctta gtagttttac ggtcatatca
    28021 aggctcattc aatcgtggta aattcgtggt aagatgagtg tttttctata cttcaaaatg
    28081 cttgaatgta tagtgtttat gcggataat
//
LOCUS       DACCXW010000025        27410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCXW010000025 DACCXW010000000
VERSION     DACCXW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.025"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            124..792
                     /locus_tag="IVY71_000887"
     CDS             124..792
                     /locus_tag="IVY71_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7984955.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            795..1838
                     /locus_tag="IVY71_000888"
     CDS             795..1838
                     /locus_tag="IVY71_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7984956.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(2075..2956)
                     /locus_tag="IVY71_000889"
     CDS             complement(2075..2956)
                     /locus_tag="IVY71_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7984957.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            3115..3843
                     /locus_tag="IVY71_000890"
     CDS             3115..3843
                     /locus_tag="IVY71_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7984958.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            3999..4316
                     /locus_tag="IVY71_000891"
     CDS             3999..4316
                     /locus_tag="IVY71_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7984959.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            4309..4917
                     /locus_tag="IVY71_000892"
     CDS             4309..4917
                     /locus_tag="IVY71_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7984960.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            4933..5763
                     /locus_tag="IVY71_000893"
     CDS             4933..5763
                     /locus_tag="IVY71_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7984961.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            5901..6389
                     /locus_tag="IVY71_000894"
     CDS             5901..6389
                     /locus_tag="IVY71_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7984962.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            6440..7312
                     /locus_tag="IVY71_000895"
     CDS             6440..7312
                     /locus_tag="IVY71_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7984963.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            7488..7850
                     /locus_tag="IVY71_000896"
     CDS             7488..7850
                     /locus_tag="IVY71_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7984964.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            7859..8611
                     /locus_tag="IVY71_000897"
     CDS             7859..8611
                     /locus_tag="IVY71_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984965.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            8625..10427
                     /locus_tag="IVY71_000898"
     CDS             8625..10427
                     /locus_tag="IVY71_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984966.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            10704..11744
                     /locus_tag="IVY71_000899"
     CDS             10704..11744
                     /locus_tag="IVY71_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7984967.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(11796..13940)
                     /locus_tag="IVY71_000900"
     CDS             complement(11796..13940)
                     /locus_tag="IVY71_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7984968.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(14297..15670)
                     /locus_tag="IVY71_000901"
     CDS             complement(14297..15670)
                     /locus_tag="IVY71_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7984969.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(15670..16065)
                     /gene="rbsD"
                     /locus_tag="IVY71_000902"
     CDS             complement(15670..16065)
                     /gene="rbsD"
                     /locus_tag="IVY71_000902"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7984970.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(16093..17010)
                     /gene="rbsK"
                     /locus_tag="IVY71_000903"
     CDS             complement(16093..17010)
                     /gene="rbsK"
                     /locus_tag="IVY71_000903"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7984971.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(17118..18131)
                     /locus_tag="IVY71_000904"
     CDS             complement(17118..18131)
                     /locus_tag="IVY71_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7984972.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(18212..18811)
                     /locus_tag="IVY71_000905"
     CDS             complement(18212..18811)
                     /locus_tag="IVY71_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7984973.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18997..19698
                     /locus_tag="IVY71_000906"
     CDS             18997..19698
                     /locus_tag="IVY71_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984974.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            19708..22419
                     /locus_tag="IVY71_000907"
     CDS             19708..22419
                     /locus_tag="IVY71_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7984975.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            22870..24498
                     /gene="treC"
                     /locus_tag="IVY71_000908"
     CDS             22870..24498
                     /gene="treC"
                     /locus_tag="IVY71_000908"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7984976.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(24558..25739)
                     /locus_tag="IVY71_000909"
     CDS             complement(24558..25739)
                     /locus_tag="IVY71_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7984977.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            25991..>27410
                     /locus_tag="IVY71_000910"
     CDS             25991..>27410
                     /locus_tag="IVY71_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7984978.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT         8863 a         4837 c         5612 g         8098 t
ORIGIN      
        1 ttgaaatatt cggtatacta ataagtatat cagaacaatg atcgtagtat acgaaataaa
       61 tgttaactga agatgaacta caagaaaaaa tctagaaatt tttcaaaaaa cagagggaga
      121 tcaatgaatc gtattttagt agtagaagat gatccgcaat tacagttgct ttaccgttct
      181 gtgttggaac gagcgggctt ttcagtcatc accgcattga atggagagga tgcgctgaaa
      241 aaactagaag cgacacgtat agaattaatc attacagata tcatgatgcc gaaaatggat
      301 ggctatgact tgctagactc attacgtagc accagatttg acacacctgt tctaatgatt
      361 acggcaaagg ccgattttga ggataagaaa cgcgggttca aattaggagc agatgattat
      421 atgacaaaac ccatcgatgt caatgagatg atcctacgtg tggaagcatt gcttcgacgt
      481 tcaaagatca attacagcag tagcttgata atcggacaaa caaaattaga gctagatgct
      541 tatacagtgt tacaagatca gcattcgttg attctgcctc aaaaagaatt tctgcttctg
      601 tataaattac tgtcttatcc caacaagatc ttcactcgcc aacagttgat ggatgatatt
      661 tggggattag atacgaatac agaagaacga acgatcgatg tacatatcaa acgtttaagg
      721 acccgttttg aaaagacgga agattttcaa atcatcacag tacggggact aggctataag
      781 gcggttattt aaagatgaaa aaaaagctat tttcacaatt atggatctat tttgctagta
      841 ttgtcttcat ttcgatctta gtgacactgc tgtgttttct cggttttatt ttcttattgt
      901 ctcgacattc tatctcgcct gctgaatcgg ctggaaaacc aacgttatac ccgttattcg
      961 tctttgcggg gttcagtatg gtcgtgggta caggaatatc gatttttgtt gggcggcgta
     1021 tcctgcatcc aatcagtgcg ctagggacaa atatgagtct ggtagcaacg ggagattttt
     1081 cgattcgaat ggatgaacag cagaaagtag cagaagtaca gcaactgtat aaagatttca
     1141 acgttatggt ccaagaactc aatagtatcg agacactgag gaatgacttt gtgtccagtg
     1201 tatcccatga attcaaaaca ccactagcaa caatccaagg atatgttcaa ttgttacaag
     1261 ccccaaatct ctctgacgaa gaacgccaaa tatttttata tcggattatc gaaagtatca
     1321 cgcagctttc acaattaaca gagaacatct taaaattgaa caaactggaa aaccagcgta
     1381 tccaactgga aaagaaagag tatcgattag atgaacaaat cagagaagta atcgtttttt
     1441 ttcagcctaa atgggagaaa gaacagctag aattggatat cgaacttgct gcagtaaatt
     1501 acacaggcaa tgaagagttt ttgtaccaag tgtggctgaa tatcatggat aacgccataa
     1561 agtacaatca aatcaatgga cagattcata tcaaactatt tgagacagcc acagagattg
     1621 tactggaagt gaccgattca ggtgtaggaa tgaatgaaga gaccagagat cgaatgtttg
     1681 aaaaatttta tcaaggagat acaagtcgtc aaatttctgg gaatgggcta ggactttcac
     1741 tggttaaaaa aattctagaa ctgcatgatg gacgtattga ttatagcagt atcgaaggag
     1801 taggaacgac agcaatgata cggttaagta aacaatagga aaaagaataa aacccctaaa
     1861 aattctattg aaggttttta ggggttttta gtcgtttttt tgtgattagg gaaatgttct
     1921 tacggttctt ttggcataaa aatcgctgct ttcttattga actacgagaa atgttagagg
     1981 tgtcattgaa acaaggacat gatgcaagaa agagagcgaa agtcatgcgt cgctctcttt
     2041 ttttgggtgg atagatggac agaaaatgta gggcttagtt ttcttcttga tttaaatact
     2101 cccaaaatgt atctgaagcc aaagaatgct caccgacttt acgagtaaat gcaagataac
     2161 tctctgttgt tggagaaata gggagaatca cagtattttc ttttaataga ttcttgactg
     2221 ttttttccat aacaatagca atccccatgt catttgcaat catattgatg atcaagtcga
     2281 ttcttgctcc tttataagag atttttggtt caaatccagc ttcgcgacac agatctagta
     2341 caggttgata caagttcgta gtggggccaa ggagcaaaaa gttctccttt ttcagttcgc
     2401 ttagatccaa tttcttttta ctagctaaag gatgattttt aggtagaact gctacaaaat
     2461 cgtctgtttc agtaggcaac cactcaatcg aacttggtgg ggcctggaaa cttctagcaa
     2521 agtggatcgt gtcactatgt tcattggcgt cattgtaagt taattcgaca ctttccactt
     2581 ctttcaactg gactgtaaat tcaggatggc gtttcaaaaa ctcggtgatt ttggaaaaac
     2641 ctttataatt gatcattgtc ggtatcgtta agatatttaa tgaaagattg ttgtctttcg
     2701 tataggtttc aagcacttct tgcatagcat ggtattgttc tacgacattt tttacatatg
     2761 ggagcaataa gcttccagct tcggtcaatt cgatttttct atgggcgcgt tcgaataatt
     2821 tgacgtctaa ttctgtttct aacgaaagaa tctgtttaga aatatttcct tgggtggtga
     2881 attgtcgatc agcagttttg gtgaaattca ttgtttcggc taaatcaaga aaaacttcga
     2941 atttatggat atccatcata ttcctcctac ttattccaat aaagaatact ttaacacaaa
     3001 aaatgaattg gtgtaatctt ctctgtctgt tacaatcttt cttgcagcga aagcaataat
     3061 tacagaaatt tttctgattt ttttcgcttc gtagaaaaag agaggagttt tttgatgcca
     3121 actggagttt tgatcaatgt aggttccgtt ttgtttggcg gactaattgg cggattagta
     3181 ggaaataaat taagtgaaca ttttaaagca cagctgacga tggtttttgg tgtgtgttcg
     3241 atggggatgg gaatctattc cattgcgccg atgaaaaata tgccggctgt tatttttgct
     3301 ttagtgatag gaacagcgat tggtttgatt gttcatttag gtaatgggat aaataagggg
     3361 gctgcgctga tgcaagtacc tatttctaaa atcttcccta gtgaaaagct tggtatgacc
     3421 cacgatgaat ttatcagcac attagtgacg gtcattgttc tattttgtgc aagtggtacg
     3481 gggatctatg gaagtttaga ttctgggatg acaggagaca gtacgatttt gatttctaaa
     3541 tctgtccttg attttttcac agcagctatt tttgcgtgta atttagggta tgtcgtttct
     3601 gtgatagcaa ttccccaatt cattattttt tatattttgt tcttattggc aaaatttatt
     3661 tatccattga ctacaccaga catgatctta gattttaaag cttgcggcgg attcttgatg
     3721 gtcgctaccg gttttagaat gatcaacgtg aaaatgttcc cagtagctga catgatacct
     3781 gcaatgatcg ttatcatgcc actgagctgg atgtggacaa attggattat gccactgtta
     3841 taataaaaat caaagagaag ccattttttg ctgactaaaa aggatcaaat cttttagtca
     3901 gtttttttgt atttgacaaa ctgattttga aactatatta tatgttgtat aatatagatt
     3961 gaaataaaat attacacttg agaatagaag gtgacgtaat gcacatccag attccaactg
     4021 ttcttcttga tggtactgtg ctagcagcat taagcaagga agatatgtat gggtatgcct
     4081 taacaaaaat cgtacaagaa agtctctctg tcagtgaatc tacactatat ccggttttga
     4141 gaagattgaa aaaagaagga tatcttgaga cctatgatga accttttgaa ggcagaaacc
     4201 gtcgatatta ctcgttgaca ccaatgggaa gtcgtcattt agaagaaatt attgatgaat
     4261 ggattgtttt tcgagattct ttagaccggt tattggagga gagcacaaat gaatgaatat
     4321 ttgaatgaag ttgtggatca tcttcaagaa attcctgaag acgatcgttt tgatttgatc
     4381 cagtattatg aggaatattt tttagattct ggaaaaacat tagatcagat cattgatgaa
     4441 tatggtacac caaagcagtt tgccttaaaa ttaaaaatca gttacttttc agaagaagaa
     4501 caagcagaaa acgagttagt gaaaaatcca aaaagacaaa ttcgcctgat ttggttaatc
     4561 attatcggtt tatttgcttc tcctgtcctc attccactag cactggggtt tatattggtt
     4621 attgggggac tattggttgc tttactagcg ctcattttta gtatctatgt tttatgtatc
     4681 agtatcttag gtgttggcct attcatgatc atctcaggaa tcagtgtgtt tggacaatcg
     4741 attctgagtg gattattctt tatcggtgtc ggtctgttcg ccacaggaag cgcagtattt
     4801 atttcgccac tgttgctgaa aggaacaaaa gggctttttt atgtcatgat gaactttgtt
     4861 aaatggatgg gacgacgttt tattacaaaa agagatctgc attcagcggg cgtttgaagg
     4921 aggacacata aattgagatt gaaacatttt ttgattaccg gtattggtct gatgcttact
     4981 ggtggcctcc tagcaggtgc aacttataca ttaggcgctc aaaagagtct gacttgggat
     5041 aatggtcctc ggttgatcga tatggttact gagacaaaaa aaatcaagga tgcaaacaaa
     5101 atcgtcatcc atgcagaaaa tcaaacagtt tcggtcatac gcggattgga ttttgagata
     5161 aaaacgactt atgataagac agaaaagcca gaaataaagg agaaaaatgg cgtgttgact
     5221 gtgaatgcag tcaaaaaatc aacaggtgca atcattagtt tagaagacac aagtgcacaa
     5281 atcactattg ttgtccctgc agatcatgaa ttatctgaat tgacaatcga agggaataat
     5341 agtgcaattg atatcaacga agtatcaatc aaaaaagtga atactaagac acatgatggc
     5401 tgggtatcgt tcaatgaatt tacaggagga acaataactg cgaaagatga attaggctca
     5461 cttcgattag atgaggccaa tgtggataac ttgatcgtca caggtgaaaa cagcaacttc
     5521 catctagaag acagtttgat cagcaaagaa ggaaaagtca tcctaaacgg tggcaattta
     5581 tctttgcatg agatggaaaa cagtggctat actatccaaa cgtctggtga atcttatatt
     5641 gaaaaagact accagccaat aacaaatact ttgttagaac aaggacagaa aaagctaaag
     5701 atcactgcta aagacacatc gatcaatatt acgacggatc aagaggacaa tgaaacagat
     5761 tgatcctgta ctcaactgga tattttaaga aaaaacagca aagatccttc caatctttgc
     5821 tgtttttcat cgcttaacgc acataactat agtaaaataa gaaagaaaat atctaaaaag
     5881 aaaacagggt gaaagagtat atgaaggtag aagaaggtca aagagaaaag ttaaaaacag
     5941 aaatggaaag attacatact tatatcactc aactaagcca aaccttctat gatccagaca
     6001 aagaaaaagt gatggttaat tacccgaaca attcagaagg tcgccaatta gagcaagtgt
     6061 accatgaagt tttcaaacat ttattgacgg tgaagaaaga gctggattat tattctttac
     6121 cgattatcga tacaggaata ttgaagtatg atgggaagaa ggaacgattt atctttaaat
     6181 ctgttcggga agatttgcct ctttcagcag ggatggatct agaggtcttg gtagaagact
     6241 attttacaga agaaaagcac tgggttcgaa caaaattgga ttatttaccg caggcagccg
     6301 gagggacaca ggcaagcggt tggtacatca ctgaagacaa agagttagaa ctagaaggtg
     6361 taatggcgcg tattcgaaaa aaaaattaaa aaagtaacat tagatattta ttgaaggata
     6421 aaggagcgca ggaagtagca tgagagaaaa aagagcattt tatatcaatg gttatgcagg
     6481 gattgtcgga ttggtcgtat tagctttagt tggattgttt ttcttctatt taggtatgtg
     6541 gcaagcgaaa gtttgggcac tttttttaag tatttttctt tggttgatca cgttattgtt
     6601 gttaagttcg gcaactgtag tcagtcctaa tcaagcaaaa gtaattttgt tttttggaca
     6661 atatttaggt acgattcgag aaaatggctt tttccttacg atacctttgg cacaaaaaat
     6721 gactgtttcg ttgaaagttc ggaatttcaa tagttctgtt ttaaaagtca atgatttgga
     6781 tggaaacccc atcgaaatct cagcagtagt agtctttaaa gtcgttgata cagcaaaggc
     6841 attattcgat gtagcgtatt atcaagattt tgtagaaatc caaagtgaga cagccattcg
     6901 gcatatcgct agccaatacc cgtatgatac attcaatgag aatgatttga cgcttcgagg
     6961 aaacacgacg gcagtgtctg acgagttgca aaaagagctg caagagcgtt tagcagtagc
     7021 aggtgtcgaa gtaatcgaaa cacgcttgaa ccatttagcc tatgcaacag aaatcgctag
     7081 tgccatgctc caacgtcaac aagcaaaagc cattttgtct gctcgacaaa ccattgttga
     7141 aggagctgtc acaatcactc aaatggcctt agagcaaatc gaagatggac aagaaatcaa
     7201 tttcaccgat gatcggaaag tccaattgat caataattta cttgtctcaa tcattacaga
     7261 caaaggaacg caacctgtta tcaatacagg cgacatgaaa gaccgtaagt gacatacttt
     7321 ttgtaagata ggaagaagga gtttaggacg gattattgtc ttgaactctt tttttagttg
     7381 ttttaaagaa aatgacaaaa ttgaaagaag aatgatagtt gattcgaagg cagaattgtt
     7441 cgcttttttc tataatcagt atataaagta agagagaagg ggaaaaagtg aaaaaacttc
     7501 tgatcggtct acttgttttc atcgttgtgg tacttgcagg ggtgaaaatc gcagattacg
     7561 tagttatggg tggagaagaa tactatgtac aaattacgac agatggtaca aaaacagttt
     7621 caaaatcaga ctctggacaa gaaacagtca actatagata tatcctgcct ggctatacga
     7681 aagagggtaa ggaaaaagaa ctggacttca agggacaatt gactcgaccg cttcgtaaag
     7741 gagcatattt aaaaatcacc tggaacaaaa ataaaggtgt cacttcgtat gaagaagtcg
     7801 aaaaaaaaga cgtgccgaaa acagcactgg aaaaactaat taaagggtag ggaaagaaat
     7861 gaacaaaata gtcgaagtac agaatgtaac aaaagtatac ggaaaaaaca atgagaaaaa
     7921 gacgcaagca ttaagcggca tcagcttcga tgtagaaaaa ggcgaattta ttgggatcat
     7981 gggcgcttct ggttctggta agtcgacact tttgaatatt ttatccacat tagataaacc
     8041 aacagatggg catatccgaa tcaatcaaaa agatgtgacc acattaaaag gaaatcaact
     8101 agcagatttt cgtgcgaatg agattggctt tattttccaa gatttcaatt tattagaaag
     8161 cttgacagca caagaaaata tcgctgttcc tctttcttta caaggcgtca gaccaaaaga
     8221 aatcaagcaa cgtgtccaaa aaattgcaga acgtttatcc atctctcata ttttggaaag
     8281 ctatccttct gagatttccg gtggacaaaa acaacgggtc gccgccgcac gtgcattgat
     8341 cacacaacca acgattctat taggggatga gcctacaggt gcgctggatt ccaaaagtgc
     8401 ccgggatctt ttagatacga tggacgaatt gaatacaaaa gatcatgtat cgattcttct
     8461 tgtgacgcat gatccgtttt ctgcaagtta ttgccaacgt attttattca tcaaggatgg
     8521 cggtatccat caagaagtga aacgtggaga tcaaagccgt gattccttct atcgagaaat
     8581 actatctatt cttggaaact tggaacagta gggggagatt gactatgctt tggaaattat
     8641 cacttactgg gatcaagagc cgtttaagag actacttggt gttattctca ggattagtta
     8701 tggcatctgc tattttttac atgtttgaat caatggcaag caacgaagct tttttgaaaa
     8761 gtaatacgat cgttgcggct acagttatca ttttccgatt cggttctgtt ttactaggga
     8821 tcatcacatt cgtttatatt ttatatgcaa attccttttt gatgacgatg cggcaaaaag
     8881 actatgcaat gtttatgatg ctgggagcaa aaggcagaaa aatcgcacag atgatttttt
     8941 ctgaaacatt cttagtaggg atcgcagcta ctttagtggg ttcagctgtc ggtgtaggat
     9001 tgacaagtat cgtccaccga ttattagtgg atcagttaga tctcaatatt tcgcatttta
     9061 caccttttag tattcacggg ttgttgatca ctgttttgtt ttttgctgtt ttgtttttat
     9121 tagctgctgt tgtcaatgct ttttcgattg tcaaaaaacc gattttagca ttgattcgtg
     9181 cggatcagac accaacaaga atcaagcagc ataaattctt gtttttcctt gaagtagtac
     9241 tgggaattgc ttttctaggc tgcggttact atatgatgaa acacgtgggg acctatcaag
     9301 tctttggtat aggggtagcc ttagttacga tcgtattagg aacttatttc attttccatt
     9361 ctgtgatcat tttcttttta tcattactaa agaaatcaga acagatttca ttgaaaaaat
     9421 tgaacaattt cacattgtca cagttaaact ttcgtattcg tgaatataca caaatgctct
     9481 caatggtcgc tatgctgttt gccttggcac tgggcgcttt gacggtagga ttaggtttta
     9541 aaaatgaaat tgcagacttg acagaaaaca attctggata tgatctagtg atgaataatc
     9601 ctcgggcaga agatcagcaa aaaatcaaag aactttcacc aacactttct gcttcttata
     9661 ctcaaaaaga agatgcggga acgatttact ataatgcaag tgaatttgcg gctcaaccat
     9721 tgatcatgat aaaacatgaa gatggaacac cgccgaagat cacgaaagaa aaagttcctg
     9781 ctaaaaaaat gaatgaactg actgtacaag aggaacttcg aagctatgaa ttgccggaac
     9841 agcaagaaaa agaaatcaag gtagtcagtc aatcagaatt tgatcaactg aacttgccac
     9901 aatccacttt acagctagtt caggtaaaag acttccaagc aaacctgaaa ccaatcgaaa
     9961 cacttgttgc tcaaaataaa atgaacaatc catctttgca aaaagtagag tacagcaacc
    10021 agaaatatga aatgtatgaa gtgtataatg gtctattctc tggttttgaa tttatgggat
    10081 tcttcctagg tttagctttc ttgaccatgc ttgctagctg tttgatgttc aagatcttgt
    10141 caggaagcaa gagtgacatt gctcgttatg aaatgcttga aaagatagga acacgacgcg
    10201 ggttgcttaa acaatcgatc cgtagagaag taggcgtgtt attcttagca ccaggaatct
    10261 taggtgtagt ccatgtctta ttcggattac aaatgtttgg tctattcatg caaaatccat
    10321 acaaagatat ttgggttccg tttacgatat tcttcgtatt gtatttcatc tactatgtac
    10381 tgacgacatg gttatatact ggaatcgtat tgaaaaagag attgtgaaaa agctgtcctg
    10441 catcaagtaa taagaacagc caagcaaaaa tagctcctca tatttttcgt ttggttgggc
    10501 ttattaccgc agatgcaagc ttttgaacac cgtttgttcg gaaaaagagg ttgtgacaat
    10561 atttttgtca caaccttttt ttcacggtct tttattagaa tatagtaaat tttttctcgt
    10621 tactcacgga ataagctgtt aagaaaacgg tttccatgta tactggaatc agtgcaaatg
    10681 aaagaaaaga gggtgcaagg aacatggaaa tcaatgaaca aaattttggc gaaaactatc
    10741 acttgattac gatcacgaat caaaatggga catctttgtc tgtcagtgat cttggggcac
    10801 gggttgttca ttttcaaaca ataatcgata atgaaaaacg agagttgatc ttaggttttg
    10861 atgaagcaaa agaatatcta gaaaaagacc cttatatcgg tgcttcaatc ggacgtatgg
    10921 ctggacggat tacgaatggc acattcaaat tagatgatga aacgttcgaa gtaaaaacag
    10981 acccggacac tggtcattgt ttacatggtg gtgcacctag ctttgaagcc aagcagtggc
    11041 attatacagt catcaatggt gaaaacgaag catcggtcat tttctatcag actagcccag
    11101 atatggaaaa cggatttcct ggaactttag atgtagaagt acggtatacg ctgacgaacg
    11161 atgatacatg gcgagtgacg gtccaaggga tcagtgaccg caaaacagta ttcaatccta
    11221 caaatcatgt ctatttcaat ttaaccggtg atccagcaca gtcaatcgac gaacatatac
    11281 tttggttgaa tagccgatta tttgctacgc tcaataaaga tacaacgcct acaggagaag
    11341 tagtttcagt aagtggcaca gcatttgact tccaacgtcc taaaaaatta ggtgaggtct
    11401 ttgccagtga ttttcgccaa tcaatgatag tgggcggcat cgatcatcca tttttgttga
    11461 aaaaacaatc tggtttacta aatgctgcgg aattgacaag cccagatgaa aaagtatcga
    11521 tccaagtaaa aacagatgct tcaggaatcg tgatttttac agcaaatttt ggagaagatg
    11581 gaccggaaat gcgcgggaat aaattagtaa atcatggcgg tatcacattt gagactcaag
    11641 aactgccagg agcggagcga cttgaaagtt ttggagatgt cactctggaa cctaaccaag
    11701 tacgcgagtt tgtaacggaa tacaaaataa atataaagaa atagaataaa ttaaaaaggg
    11761 agtgtgacag tgtcacatcc cctttttaat ttattctatt tctttaaagg cagatcaagc
    11821 cagaatgatt tgtctaacct taacaaggtt agtacaggaa cagttgcagc tttaactgta
    11881 cgagtagttg tttctggtat ctgtcttgcc gtaaaattgt tccacaaatc ccagattgaa
    11941 gcgacacgga agatggaaga agcgttgttg acgcgtttta tcgttgaaaa ttctaatcct
    12001 gtgattggca cgccatctgt tgtccatttg attggcacat cgaatcgctt agacatattg
    12061 aggatttcag cgtttgcttc ttcagttgtt gctgcttctt ttttcggttc atcatatttt
    12121 gtcaggtcgt acgcttcgat gatcgcgttc aatttttgtg aatattggcg atctgtagca
    12181 taacgtcctt ccaaatattt ggttgcttct ttgtagtctt ttgtttccga cttccaagtt
    12241 ggtttgtaga aatcaggatt gccagtcagt ccatttttga tcaagtcggc atagtcttcc
    12301 aaactttcct tgtaagaagg atatttacgg aattgtgcgc ggatttggta gctttgtccg
    12361 gaactatctt gttcaagtgt attgaaggtc acgctgcttc ctttatatga tcctttgata
    12421 ccaaaaaggt tatgatttgg ttcgcttgat aataagctgt ttccagatcc actttcaagg
    12481 atagcttgag ccatcatgac tgatgcgtag attccttctt tttgcccgat ttcctgtgca
    12541 tcatctgcaa tctttttcaa gaactgatca gttgtttggt tttgactgaa atggaacgag
    12601 ccatctaatc catctaaaat agatgagccg cctgtcagcg aacctgaagc agcaggtaaa
    12661 cctgctaagt ttactcggac agcaaaatca ggcgtgaaat ctgaaataga agttactcga
    12721 actttatctc ctggttgagg cgcatggata aattgtccgt tcccaatata gatacctaca
    12781 tggtgagtaa caccggaaga aggtccccag aagacgaggt cgccaggttg ggcctgactt
    12841 actggaatct gcgtacctgc atattgctgt tcgcctgtcc agccaccgat ataatgaccg
    12901 gttgctttca tatatacgta ataagtcaaa ccagaacagt caaaagtatt tggacctttt
    12961 gctccccata catatggttt tccaagatgc ttgaatgctt ctcgaacgat tgcttgttgg
    13021 acattcgttg tatttgtagg caatacagca tcaattggtg attttacaac aaaatcagaa
    13081 gaagcagaag atggattctc tataccgctt tgatttgctg tatttggacg agatgaagca
    13141 ttgttcgttc cgccattcga tgaaccattg tttggatggt ttggttctct ctgagtattt
    13201 ccattgttat tcgatccaga tgggtttgtt tgattcgaag aattcccgct gttcgaatta
    13261 ttgttattat tgtttgaatt atttgaacca ttattattgt tgttattacc cacatctggt
    13321 ttgtttggcg tttccggatc tggtgtacta gggacttctt cactaggatc agttggtttc
    13381 tctgttgaag tatccggtcc agctttaaat tctgcagtaa tagataaatt gttcgtacct
    13441 actgtaaagg tctttgcatc ataatttact tctttggcac cattaacagc gactgaagca
    13501 atttgttcac cttcagctgg cgtaatagtg taagttacct tcgtttccgc taataaacct
    13561 tctaccgttc cactttccgt aatatccaaa ggttgctgtt caggggaatc cgctgtcgaa
    13621 gcagtcagtt ggatcttacc aatcaaagaa ggagcaattg taagggaata tgtctcggct
    13681 ggcttttcat tttctgatgg atcagccgga tcaatttcag ttggctcggt cgatcctgtt
    13741 gttgagttag ttgttccagt tgggtcttct gtaaaagaat ccgttgtttc actagtagct
    13801 gagctggatg tagtcgtatc tgttgtgact gtttgatcag ctatttgatt atctacggca
    13861 aatacggtat tggtcgacaa aacagacgga gcaatcagcc aagtagcagc tagcaaacgt
    13921 atcgtttttt tatttttcat aataaactag tccttttcct ttcattgatt ttctgtctta
    13981 ttcagcattc attagacatt ttttggaaca gataatcgtg tcgaaaagct ttttcttttc
    14041 ataaatctgt ctgtgtattc taaataaaac aatttgtaaa taagcaacga gcattcattc
    14101 gtttttcttt gacgttcatt ttattgcata gaccaccttt gtttggggat aaacaagtgg
    14161 ggataaaaat ggttatgcat atgtaaatta taccaaagtt tggaaagtat cacaaaaatt
    14221 tataaaaata atgatcctaa aaagaaaaag actgaaatat gtgataaaca tctcatactt
    14281 cagtcttttt cattaactat tcataagaca ttaggtcttc atcaaacccg ctaacgagag
    14341 tagtgatcat tgctacgact agactaacag ccagtcctgc taagtaccag aaaaaagtac
    14401 cggaaatgaa tgcaggcaat gtggtcagac taccaaatac aaaagcgatc gctttcactt
    14461 gggcaaatcc cattacagct ccaccagcag ctgcaccaat gcaagcagca acaaatggtt
    14521 ttttcaatgg gatcgcaatt ccgtaaacag ttggttcggt cactcccgca agtaaaatcg
    14581 aagcaaaagc agaaccggag atcgaacggg tttttgaacg tttacttcgc agaaatacac
    14641 ctaaagctgc accagccatt ccaaaattgg ctgctcctgt caacggaacg attcggtcga
    14701 acccataagc agagatatta ctgatcatga cagggtttac agcccattgg ataccaaata
    14761 ttaccaaaat attccaacca ccagcgatga ttgcgccact gatcggtccg ttaatatcca
    14821 tcatccaagt aattgcggca gctaaacctt cgccaaggta aacacccgca ggcccgaata
    14881 ccattaacgt caaaggaacc gtgataacta atgaaagtaa aggtacaaat actaattgca
    14941 tattttcgcg aatatatttt ttgaggaatt tttcaacaaa agaataaaag aaaataccta
    15001 aaatcgccgg catgacagta gacgtatact gcatcagtac tacaggaatc cccagaaaat
    15061 cagtagctgc tttgccactg tctgaaagta aagaggtcag attcggttca atcagtgctg
    15121 cagcaatgac agccgcaata tatccattca ctttaaacgt tttggcagca gaaaaagcaa
    15181 gaaagattgg cagaaaataa aagacggcat tcgacgcagc agacaaaata tgataagttc
    15241 cgcttgtttc actaagccag ccaatcgtag tcgctaaaac aaccagacct tttaatatac
    15301 cagaaccggc aaacagcgga atgatcggcg taaagatttc cgatatcaaa ccaaatagtt
    15361 tttcgatcgg atttagcttt tctttcggag ctgaatgatc gatttgacgc gtcaattcct
    15421 tgtaagcagg agccaatccc tgatccatga ttatttctgc atatctatcg agtaaaatga
    15481 aatacttaat ggaagggatt tgctgtttta atttttctgc attggctact ggctgttttt
    15541 tcaatttgat acgaagccga gtcgcacatg gaaggacaga atcaatattg tcttttcctc
    15601 ccaactgttc aatcagcata tgaatctgct cggacagtac catctgttca ggattcattg
    15661 tcgtttccat tagaagacca ctccactcac taatataata ttggcgtaag gtgtcatttc
    15721 gcctgtacga ataaatgcat gacagttatt caactctttt ttcatttctt catgtgggat
    15781 aaagctaatc ggtgtatctg gtaacaataa tttgattttt tccaatattt ccgggttcat
    15841 tgtctttatt tcttccgcca attcaatcga ttgtacctca agttcttcta aaacgttttc
    15901 taaaacagat aaaaaacttg gaagaccgtt tgagacagcc aaatctattt tttcagtacc
    15961 aaaggggaca ggcatgcctg catccccgat acttaattta tcaaagtgcc ccatttgtgc
    16021 gattactcgt gaaatatctg aattgattac ttttcctttt ttcatgtttg tttcttcctc
    16081 tcattgttaa ttttagactg ctgattgttc taaagcttcg cggtaaggga tagaaggttg
    16141 cgcaccataa gactgcacag caacagaaga ggctagattt ccataaagga tcgcttcttt
    16201 caagttacta aggtctggct gaagtgtgct gcttaatgcg ccaataaatg tatcacctgc
    16261 ggctgttgtg tcaacggcat ccactttaaa agcagggacg ataccgtgtt ctttttctgt
    16321 atgatagaaa gcgccggcac tgcctagtgt aatgattact gttccaatac ctaattcatg
    16381 gagtttttcg gctgctgcga ccaaactgtt atgatctgtc actctgattc ctgtaatgat
    16441 ttctgtttct gtttcatttg gtataatgat atctgtcttt tcaagtagtt cagtaggaat
    16501 cgtttcacga gctggtgcag gattcaggat cgttgttttt cctgcgtctt ttgcaataga
    16561 aaaagctagg atcgtcgcat ctaagctact ttcaaactga gcaataataa agtcactgtt
    16621 tgtaatcagt tgcttgtttt ttaggacttc ttgttcatgg aaagcattat ttgcccctgc
    16681 atggatcaag atactatttt ctcctgctgc gtctaccata atcattgctt gtccggttgt
    16741 ggcgccttgg atttcagcta tcccagatgt atcgatgttt tcttttgtca gtagatctaa
    16801 taactgttgt ccttcgctgt cagccccaac gcctccaata aaagatgtcc gtgcgcctga
    16861 acgtttggct gccactgctt ggttggcacc tttacctccg ccagcgtgaa aaatctcatg
    16921 cgcatgcatc gtttcgcctg gcttgggcat gttggttaaa cgaagggtag tgtccatatt
    16981 aatacttcca attactgtaa ccgtgttcat tttacaaacc tccaactatc tcatttattt
    17041 tgatgggaaa attatttttt aaaacgtttt agtaaaacgt tttaatagtt cgataaaaat
    17101 atagcacacc ttgaaagcta tttcaagggt gcagttgaaa atcttttttc taatcgtaca
    17161 ggtaatcttt tttcttccca ttctttttct gggaattgga tccgatctaa caataacttg
    17221 gcagatgttt gtcccaattc gaagattggt tgtgcgatcg ttgtcagttt cggcttgata
    17281 tactcgcaca tatctatatt atcatagcca ataatgctgt agtcttcagg gatgcttttt
    17341 ccagcttcct ctaatccgcg atataatcca aaagctaatt catcatttaa agcaaacaca
    17401 ccagttgcag cagaatcaag aagttctgct gtgatctggt agccaccttg ttttgagaac
    17461 tgtgtaggta agaggatcag ttggtcgtga gggatttggt aaacatcaag agcagattta
    17521 aacccctcaa tccgagcgtg gacattttcc ggtggatttt caggatagac aagggcgatc
    17581 gtttgatgtc ctagactaag caagtgcatg cccgctaggt aaccaccgcg aaaatcgtct
    17641 gttcgtacgg catcactaaa tccttcagat tttttctgat ccaaaacaat gaagggccgg
    17701 ccttgttttt tcaaattttc gttgattgca tctgtagaga cagcagaagt cgcgataatg
    17761 aatccatcca cacctcgacg tgtgagctcg gctagatatt cgatttcttt ttgatgatca
    17821 gaatcagcat tacataatat cgtgacgaaa ttctgcttat acaagatatc ctcaattcct
    17881 cgcatcagtg tactgaagaa tggattcgtg atatcaggaa cgagtacacc aattgttttc
    17941 gtttccttgg taatcatttg tctagcaaaa tagtcaggct gatagcctaa ttcttctttg
    18001 gcagctaaaa ctttctgaac agttttcgga ctgaatttct ccgtatttcc atttaatatc
    18061 aaggagacag ttgtgattga aacgccggaa agtttagcga cttctttgat tgtgatcttt
    18121 tttttcacca tgttcatact cctatctaaa tcctgtggtt attttcccat gaaatcaaaa
    18181 agaagtaaat gaaaatcacg tgtgccagta cttatttgct ttgttggata gcttcgatta
    18241 tttgtttcgt tgagcgtact cgtgcagttt tcggaaatat cttttttatg aacaattgat
    18301 gggattcttt gtcacgatca ctgcaagcat cttcagcaag cacgatatgg tacccgtgtt
    18361 gaaaggcatc taatgcagta gcatatacac cgtttgttgt cgctacaccg cttaaaatga
    18421 tagtttcgat tccacgccgg cgaagttgaa gatcaagaga agtaccaaaa aaagcacttg
    18481 gattgtattt tgtgatagtc aacatattat ctgtatcttt caaagaatcc ttcaaaagta
    18541 aatcagtgaa ttctggaggt acagaagcag tcattccacc catatcagtt tgttgactca
    18601 aataagggaa agtggagatg tctacgttca ctaaaacttt caaggcagcg gtacgtttca
    18661 atgttcttac taacaaatca ttgtttgtca gaatagatgc tgcagtgtgt ggagagagtg
    18721 taccgttatt tacgatctct ttttgcatgt cgatcgcgac aaaagcagtt tttgataaat
    18781 caaaaatcat attttccttt aaatcattca tgatgttcct tctttcaaat aacaatgttt
    18841 tttatgtaag taaatcataa atcgtttatt tgtcaactaa attaaaggat tcctttcttt
    18901 agtctagaga aattcagtgg taaaataaat aagtgtgaga aaacgcattc gatgcataga
    18961 tatagcgaga aatatcgtga aaatggagga atcagagtga agtatgtcga agtaaaaaat
    19021 gaatacaaac gatacaaaat gggagaaacg accattacag caaatgacga tatttctttt
    19081 tcaattgaaa aaggcgaatt agtgattata cttggtccaa gtggagcagg aaagtcaacg
    19141 attcttaata ttttaggtgg gatggataca cctgatgaag gtcagatcat tattgatgac
    19201 acagatattg cgcaattttc agataaacag cttactgctt accgacgaac agatgtaggg
    19261 ttcgttttcc aattctacaa tctcgttcct aatctaacag caaaagaaaa tgtggaattg
    19321 gcaacggaag tttctcctga tgcgcttgat cctgtagagg tattgaggca agtgggccta
    19381 gctcatcgat tgaataactt tccgtcccaa ttatcaggag gcgaacagca gagggtttca
    19441 attgctcgtg cactagctaa aaatcccaaa cttcttttat gtgatgaacc gacaggcgcg
    19501 ctggattttg aaacgggcaa gcaagtattg aaattattgc aaaacgctag cagacaacac
    19561 ggcaataccg ttttgatcat tacacataat tctgctttag cgcccatcgc tgatagagtc
    19621 attcacatta atgatgctaa agtaaggtct gttgaattaa atgatcatcc ttcatccatt
    19681 gatgagattg tatggtaggg ggattttatg aaaacaagaa catatcttaa agcaggtttt
    19741 cgcgaaattc ggcaatctaa aggacgattc attgcaatca tccttattgt catgctagga
    19801 acacttttat ttgtaggtgt caaaactgct ggacctgtca tgcaaaaaac gatggatcac
    19861 tatgttggta cagcaggact ttctgatttg caaatcgttt ctactggtgg cttgacacaa
    19921 aaagatattg ctcaagcaga aaagatccca gatgcaaaag tagaaacagg aaaacagatc
    19981 tattattcca atactggcaa aaacgaagtg attcaaatat tttcttataa caaaaataac
    20041 aagcagaaca agctacaatt aactgatgga aaactcccgg aaaaaccaaa ccagcttgta
    20101 ttggatgaaa aagcggaaga tgaagggtat aaaatcggtg atatttatca gattgattca
    20161 gatgaattga aagaaaaaga gtataccatc acgggctttg tccgttctcc attgttcatc
    20221 aacaatttgg agagaggata cgcgaacgtc ggaaatggta gcgtcgatta ttttgtctat
    20281 ttacctgaat ccgtttttaa aacagatatt tattctgtct tatatcttga ttttacaaac
    20341 gtaaagaatt tgaatacgta cagcaaagct tacaaagata aaatggagca aaatcaagaa
    20401 aaggcagaaa agtatttaca agatcgaccc gaagaacgct tagaagagct gaaaaaatcg
    20461 gccaacgaag aattagaacc ggcacagcaa aaaatagcgg atggcaaagc acaaacaacg
    20521 caagcaagaa cacggttaga agatgctaaa aaacagttag cgcaacagtc agcaactatc
    20581 cagcagcttc ctgaagcgca acgaacaatc gctgttcaaa cattatctca acaagaagct
    20641 caacttgctg aacaagaaag gcaattagtt gagaaagaac gtgaactggc ctctgcagag
    20701 gaagaattga atcgaaatgc agaaaaaatt gaaaatttaa ctaaaccatc atacctatac
    20761 aatgaacgtt cagaaaatgt cggttttcaa gaatttggtg atctgtctga tcgtatcgct
    20821 gcgattgcca atgtttttcc tgttttcttt ttctttattg ccgcattgat cacctttacg
    20881 acaatgacac gtatggtaga agaaaaccgc agggaaatcg ggacattaaa ggcgttaggt
    20941 tatacgaaag ttgaaatcgc aaaaaaatat gcaatctatg caagtctagc ctcagggata
    21001 ggtattgttt taggaacgat attaggaacg aatcttttgc caaggatcat ttttgagctg
    21061 tctaacgagc ggtatgatat tgggagtgcg cttatttttt atgactggcc gccgatttta
    21121 caagcagctg ccgcattctt cattgccgca tttggcgctg caatgatcgt tttattcaaa
    21181 gatttaagag aacggcctgc tgcattgctt caaccgaaag caccgaagcc agggaaacgc
    21241 atttttcttg aatacatcac tccgctgtgg tcacgcctta gctttaatca aaaaatcagt
    21301 taccgaaatc tcttccgata taagtcaaga atgcttatgg cgattatcgg aattgctgga
    21361 tgtgccgggc tgatggttgc aggcgtggga ttgaaagatt ctctaagttc tgtttcagcc
    21421 aaacaatttg gaccgatcat tgactatcaa gcaatcgtta cattggattc atccacggaa
    21481 gatagtcgtc aaaaagcagc ggatatgctg cagaagcaag aaaaagtaaa tgagatccta
    21541 gctattcata ctcaaaccgt agaattgaga aaaaaaggac atgcttcgca aagtctgaca
    21601 gtgatcgtac caagtaaaac agctgatttg gatcgtttta tccatttaac tgatcttgaa
    21661 gagaacccac gctccttgcc taaaacagga atcgctatta cgcaaaaagt agcagaacta
    21721 tttgacttat ctacaggaga tcatatttcg ctttatgatg aagatcaaaa agaatggaaa
    21781 gtcgaagtaa aagaaatcgt gaaaaactat ctaggtagct ttatttatat gtcccctgaa
    21841 tattatgctt cagttactga tcaatcgatg atggataatg catttttgct gcgtacagat
    21901 aagatgacaa caaaagaaga agaaatgctt tccgagaaac tgcttgccac agacgctgtg
    21961 acaaatacgt ctttcatgtc caaacaaatc gaaacgcaag aagaatcaat ggcgaactta
    22021 gactcggttg tcttaatctt tgtcgtatta tcaggactgc tggcttttat cgtcttgtat
    22081 aatctaacta atatcaatat ttccgagcgt gtacgcgaat tatctacaat aaaagtcctt
    22141 ggattttttg ataaagaagt cacgatgtac attgttcgag aaaatatcat ctttactctt
    22201 ttaggaatca tcggaggatt tggtgtagga tatatattga ctgacttcat cttgcaacaa
    22261 gcgtctatgg agaatgtcat ctttccatta gtgatcacat ggtgggctta tgtcttatca
    22321 gcaggattaa ctatcgtatt tacggttatt gtgatgattg ttacccattt caaattaaag
    22381 catatcgata tgattgatgc gttgaaatcg aatgaataaa aacagaggtt gtgaaatcag
    22441 cggtttgacc tgagtattag agaaacaaac ggcaaaatag tttttcatat tttgccgttt
    22501 tttgagctaa taccgaagga cagctgattg aacaccatga atcagaggtt gtaaaaaagc
    22561 cgtcctgcat cgagcaaaaa gaatactcaa ataaaattag ctcctcatgt tttacatttg
    22621 agaagactga ttgccgtaga taaaagcttt tgaacaccat ttatagaaga aacaagaata
    22681 ttttgctatt ttaaacaaga ggatagacaa atacatgtct gatctcttgt tttttattgg
    22741 gcaacagacc tcttagaatg tataaagata aattttacaa acgctttcgg ttcagttata
    22801 ataataaatg ataacttgta tatataagtt gtggtaggta ataaatcagt tgaatggagg
    22861 taaatatcca tgacatttaa agacaaagtc atttaccaaa tctatcccaa atcttacaaa
    22921 gacagtaatg gtgatggaat tggagattta aaaggaatca aagaaaaact cccgtatctg
    22981 aatgatctag gcatcgacat gatttggcta aaccctattt atccaagccc tcaaaaagac
    23041 aacggttatg atatctctga ttacacagcc atcgatccta tctttggtac gatggaggaa
    23101 ttcgaacagc taatagccga agcaaagaaa taccggatag aaatcatgct cgatatggtg
    23161 ctgaatcacg tttcgacaga acatgaatgg ttcaaaaaag cactagcagg ggaccagcat
    23221 tatcaggact tcttcatttt aagagaagag ccaacggatt gggtatcaaa atttggtgga
    23281 agtgcttggt cgccatttgg agatacgggg aaatattatt tgcacttata tgatcggaca
    23341 caagctgact tgaactggcg gaaccaagca gtaagagagg aactattcaa agttgttcgt
    23401 ttttggatgg aaaaaggggt aaaaggattc cgtttcgatg ttatcaatgt tatcggtaaa
    23461 gacgaacaat tgaaaaataa taaagaaaat gatggcaaac ctgaatatac ggacaaacca
    23521 atcacccata cgtatctaaa agaattgaac caagcaacgt ttgggacgga ttccactatc
    23581 atcacagtcg gtgaaatgag ctctacgacg atcgaaaatt gtatcttgta tacagaacct
    23641 agtagagaag aattatcaat ggtctttaat ttccatcatt tgaaagtcga ttatgaaaat
    23701 gggaataaat ggtctactcc tccatttgat tttgcagaat taaagcgact atttcataca
    23761 tggggagaaa agatgagtga gggtaacggc tggaatgctc tattttttaa taatcatgat
    23821 cagccacgtg cgctcaatcg atttgtagct atcgatcagt atcgaaaaca aggagcagag
    23881 atgctagcaa ccatgatcca tttgaaccgt gggacgccct atatttatat gggagaagaa
    23941 atcggcatga tcgacccaga ctattcatcg atttccgact atattgacgt agaaagtttg
    24001 aatgcttata agttactcct tgaacaagga ttcacagaaa aagaagcgtt tcgtcggatc
    24061 aaagcaaaat ctcgcgataa ctctcgaaca cccatgcaat ggtcagacac agaacaagca
    24121 ggatttacta cagggaaacc ttggttaaaa gttggaggca agctagaaga gattaatgta
    24181 gaaaaagaac tatcttctga agattctatt ttgtcctatt acaagaaatt gattcggctg
    24241 agaaaaacat atccaatcgt tgcacaagga gattatcatg cttatgaagc aaatcatccg
    24301 gaagtttatg gctttcttcg ccaatttgaa ggacagcagc tgcttgtatt aacaaatttc
    24361 tatgataaag atacaaggat cgccatccca gaaggattca cagaagccaa tattcttatc
    24421 gataattatt ccgatactgt tcttcatcaa agtctggagc taaaaccata ccaggcaatt
    24481 gctttgctga aaaattaaaa caaaaacaca aaacgtcgtt cctacgagct attctcgtaa
    24541 gatcgacgtt ttgtgtttta tttaaaataa tcttcttcag aaaatgaaga gttttcatac
    24601 tctcctttat cgccataaaa tttattgtag cgttgtttga atttacggaa aataaaagta
    24661 acgagtaatt tgactaacgt atagatagga ataccaaaaa taacgcccac aattcccatt
    24721 agatcaccca tgacaagtaa aacgattaag atcgtaatcg gatggatctg taaacgatca
    24781 cccattactc gaggaatcac caagtcaccg tggagaagtt gtacggcaaa ccagacaatg
    24841 acaaatttga cagccataaa agtagagtct tggaatgcaa tgatcaaacc tggaaagaag
    24901 gcgataaacg gcccaatata tggaatgata cataacaacc cagcagaaat agctaaaaca
    24961 ctagcaaatt ctaatccgat cagtaagaaa caaacccagt agactgctcc aaggataaat
    25021 gaagcaatga tttgcccctt tagaaatgaa cccaattgtt gattagcaat ctttgtcaaa
    25081 tttttaaaat ctgtacgaaa tgctggtggg acaatcgcta aaactgactg ataaaatttt
    25141 tgaggatctc tcaaaaggaa aaaagcgaca attggaccag taaataaagt aataaaaata
    25201 gctgtaacag ttgaaaaaat gttgctgatc cgttgtgcgc cactttccca ataatttcca
    25261 ataaatccag ctatatcaga cgtcacgtcg tcaatagaag cgaaaaattg attaaatgaa
    25321 tcggcaaaag gtgttttctc caaaaattgt cggagactct cttgaaaatc ttgaaacaaa
    25381 tctggcaaac tactgatcaa tgtctttgcc tgttcttcaa gaaaaggaat aagccatatg
    25441 cttccaagcc ccaataaaaa taaaataatc acataaacga taatgactga caagttcctc
    25501 gaaaggtgct tgctcaaacg tttgatgact ggttccaaaa gataatacaa gatcagtgca
    25561 aacaataatg ggggcaagat agtagcgata atgattccga caggcttgaa tataaacgag
    25621 atctgatgga aaaaataaat gatcagcgca agtaagataa ctattcctaa aacatagtaa
    25681 gaggtttttc ctcctaaaaa gcggatcaaa cgattcgttg tatctgtttc ttttttcact
    25741 ctttttcctc ctttatctat aacttcatta tagacaaaag cgggtataaa aggaaaatga
    25801 tagaggttga ttgcaaaaat atcgtcaatc attcatataa actttctcat caattaaaat
    25861 aagaaaacgc ttgacagttc ttttaaccat atgataaatt aaatcctgtt gctaaaacgt
    25921 aactattacg gtttgcgaaa agcgaatcgg aacattcaaa tgaaagaaga tgagaagagg
    25981 agtgtcataa atgaaacgat tgataggtat attggccatg ctggttttag caggtatgtt
    26041 aactgcttgc ggggcaagtg ggaaagccga ggacagtaaa gaaaaattga gcgtgatgac
    26101 gaccttttat cccatgtatg attttacaaa agcaatcgtc ggggatgaag gagaagtaga
    26161 gttattgatt ccagcaggga ctgattccca cgattatgaa ccttctgcta aagacatggc
    26221 aaagatccaa gatacagata tctttgttta taatgatgaa aacatggaaa catgggtccc
    26281 cgcaatccaa aaaacattac aagaaggcaa tgtccatacg ataaaagcga cagaaggtat
    26341 gcttttactt ccgggaagtg aagaaggaca tgatcatgac catgaacacg gggaagaagg
    26401 ccatacacat gagctagacc cacatgtatg gctagctcca agtttagcaa tcaagcaggt
    26461 agcaaacata cgtgatcagc tgattgaagc ttacccagaa aaacaggaag tatggacaaa
    26521 aaatgcagca gcctataccg agaaacttca agcactccat cagctttatc aagaaacctt
    26581 taagcaagca aaacaaagat cttttgtcac gcaacatgct gcattcaact atctagcgtt
    26641 agaatatgga ttgaatcaag tatcgattgc tggtctttct tctagtgaag aaccgtctgc
    26701 ggcgagaatt gctgaactga aacactttgt taaagaacat ggaatcaact atatttattt
    26761 tgaagaaaac gccaaagata gtattgccag aacgttagca aacgaagcag gtgtatcttt
    26821 agaagtattg aatcctttgg aaggattgac gaatgaacaa atagaaaatg gagaaaacta
    26881 tctttctatc atggaagcaa acctcgaagc gttgaaaaaa acgacagaga cagaaaaccc
    26941 gctagaagat actttgaccc ccaaaaaaga aaagagcgtc tacaacggtt attttgaaga
    27001 cgaggatgta caagatcgct ctctttcaga ttgggcagga acttggcaaa cagtagatac
    27061 gtatgtagaa gatgggactt ttgatcctgt ttttgaatat aaagaaaaat tgaatcagga
    27121 taagacagca caagaatata aagaatatta tacgaagggc tatcaaacag atattgcttc
    27181 aatcaagatc agtgagaaag agatggcatt tacgtttaag aatggaacaa cgaaacgttc
    27241 aaattatcgt tatgtaggga aagaaatctt gacctacgag gcaggaaacc gtggcgtaag
    27301 gtatctattc gaagcagaag ataaggaaag tggcgctttt agatatattc aattcagtga
    27361 tcacacgatt gcaccggctg actccgaaca ttttcattta tatgcaggaa
//
LOCUS       DACCXW010000026        27234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCXW010000026 DACCXW010000000
VERSION     DACCXW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.026"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(45..413)
                     /gene="rplL"
                     /locus_tag="IVY71_000911"
     CDS             complement(45..413)
                     /gene="rplL"
                     /locus_tag="IVY71_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7984979.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(466..969)
                     /locus_tag="IVY71_000912"
     CDS             complement(466..969)
                     /locus_tag="IVY71_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7984980.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(1379..2068)
                     /gene="rplA"
                     /locus_tag="IVY71_000913"
     CDS             complement(1379..2068)
                     /gene="rplA"
                     /locus_tag="IVY71_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7984981.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(2172..2594)
                     /gene="rplK"
                     /locus_tag="IVY71_000914"
     CDS             complement(2172..2594)
                     /gene="rplK"
                     /locus_tag="IVY71_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7984982.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            2937..3605
                     /gene="sdaAB"
                     /locus_tag="IVY71_000915"
     CDS             2937..3605
                     /gene="sdaAB"
                     /locus_tag="IVY71_000915"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7984983.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            3618..4490
                     /gene="sdaAA"
                     /locus_tag="IVY71_000916"
     CDS             3618..4490
                     /gene="sdaAA"
                     /locus_tag="IVY71_000916"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7984984.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(4581..5600)
                     /locus_tag="IVY71_000917"
     CDS             complement(4581..5600)
                     /locus_tag="IVY71_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7984985.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(5773..6660)
                     /locus_tag="IVY71_000918"
     CDS             complement(5773..6660)
                     /locus_tag="IVY71_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7984986.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(6737..7282)
                     /gene="nusG"
                     /locus_tag="IVY71_000919"
     CDS             complement(6737..7282)
                     /gene="nusG"
                     /locus_tag="IVY71_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7984987.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(7383..7553)
                     /gene="secE"
                     /locus_tag="IVY71_000920"
     CDS             complement(7383..7553)
                     /gene="secE"
                     /locus_tag="IVY71_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7984988.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(7577..7729)
                     /gene="rpmG"
                     /locus_tag="IVY71_000921"
     CDS             complement(7577..7729)
                     /gene="rpmG"
                     /locus_tag="IVY71_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7984989.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            7891..8535
                     /locus_tag="IVY71_000922"
     CDS             7891..8535
                     /locus_tag="IVY71_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7984990.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(8591..9094)
                     /locus_tag="IVY71_000923"
     CDS             complement(8591..9094)
                     /locus_tag="IVY71_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7984991.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(9107..9153)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            9691..10656
                     /locus_tag="IVY71_000924"
     CDS             9691..10656
                     /locus_tag="IVY71_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7984992.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(10755..11927)
                     /locus_tag="IVY71_000925"
     CDS             complement(10755..11927)
                     /locus_tag="IVY71_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984993.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(11914..13059)
                     /locus_tag="IVY71_000926"
     CDS             complement(11914..13059)
                     /locus_tag="IVY71_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7984994.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(13073..14005)
                     /locus_tag="IVY71_000927"
     CDS             complement(13073..14005)
                     /locus_tag="IVY71_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7984995.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            14122..15201
                     /locus_tag="IVY71_000928"
     CDS             14122..15201
                     /locus_tag="IVY71_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7984996.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            15198..15816
                     /locus_tag="IVY71_000929"
                     /pseudo
     CDS             15198..15816
                     /locus_tag="IVY71_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(15926..16153)
                     /locus_tag="IVY71_000930"
     CDS             complement(15926..16153)
                     /locus_tag="IVY71_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7984997.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(16256..18844)
                     /locus_tag="IVY71_000931"
     CDS             complement(16256..18844)
                     /locus_tag="IVY71_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7984998.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(18864..19523)
                     /locus_tag="IVY71_000932"
     CDS             complement(18864..19523)
                     /locus_tag="IVY71_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7984999.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(19572..20075)
                     /gene="trmL"
                     /locus_tag="IVY71_000933"
     CDS             complement(19572..20075)
                     /gene="trmL"
                     /locus_tag="IVY71_000933"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7985000.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(20296..21162)
                     /locus_tag="IVY71_000934"
     CDS             complement(20296..21162)
                     /locus_tag="IVY71_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7985001.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            21271..21912
                     /locus_tag="IVY71_000935"
     CDS             21271..21912
                     /locus_tag="IVY71_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7985002.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(21974..23335)
                     /gene="mgtE"
                     /locus_tag="IVY71_000936"
     CDS             complement(21974..23335)
                     /gene="mgtE"
                     /locus_tag="IVY71_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7985003.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(23353..24252)
                     /locus_tag="IVY71_000937"
     CDS             complement(23353..24252)
                     /locus_tag="IVY71_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7985004.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(24254..25051)
                     /locus_tag="IVY71_000938"
     CDS             complement(24254..25051)
                     /locus_tag="IVY71_000938"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7985005.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(25029..25733)
                     /locus_tag="IVY71_000939"
     CDS             complement(25029..25733)
                     /locus_tag="IVY71_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7985006.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            25864..26439
                     /locus_tag="IVY71_000940"
     CDS             25864..26439
                     /locus_tag="IVY71_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP7985007.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            26557..27234
                     /locus_tag="IVY71_000941"
     CDS             26557..27234
                     /locus_tag="IVY71_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7985008.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         7930 a         5750 c         4909 g         8645 t
ORIGIN      
        1 accgaaatcc aacataggat ctcagtgctt ttaaatagta aaaattattt aacagttaca
       61 gatgcgccaa cttcttcaag tttagctttc aattcttcag cttcttcttt agatacgcct
      121 tctttaactg gtgcaggagc gccatcaact acagctttag cttctttcaa gcctaaacca
      181 gttgcttcac gaactgcttt gataacttta actttttggt ctccagctgc agttaattct
      241 acagtgaatt ctgtttgttc ttcagcagca gcagcgccac cagcagcagc tacagctaca
      301 ggagcagcag cagatacgcc aaattcttct tcaatagctt taactaggtc gttaagttct
      361 aggatagttg cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc cattataatt
      421 tcctccgttt aattgttctt ttttatttaa tgctttatgc ggcaattaag ctgcatctcc
      481 atcttctttg tctgccactg ctttgacagc gtaagccacg ttgcggactg gcgcttgtag
      541 tacagatagc aacatagata gaagtccttc gcgatttggc aattttgcca atgctgtgat
      601 ttcttccaat gaagagactt taccttcgat gataccgcct ttgatttcta atgcttttgc
      661 ttctttagca aattcgtcca tgattttcgc tggtgcgact acatcttcgt tactgaatgc
      721 aacagctgta ggtccagtga atacttcgtc catgccttca aggccagctt ttttagctgc
      781 acgtgaaaga atagaatttt tgataacttt catttctacg ttagcatcac gaagttgttt
      841 acgtaaacgt gttacttcat caactgttaa tccgcggtag tcaacgacta ctactgatgc
      901 agctgctgta aatttttcgc ttacttcatc aacgattgct gctttttttg cgataattgc
      961 ttcactcatt tttgagtttc acctcctgaa ttttgatgga agagtttttt gcactgaaaa
     1021 ggcaagtcac ttttatagtt actttgctca accagtatta ttcatcacct attcacttcg
     1081 ttcatagtga ttctactcgc tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt
     1141 attgttcatc atctcttcac ttcgttcata gtgattcaca ataaaaaact ctatgccacc
     1201 gcagacatag agggactatt gaatatattc aataaacgtc ctcggtagga aattaaggct
     1261 tgatgccacc tactgtcttc ggtacaagtt aattattaac ctcaactagc ttagcatgct
     1321 aaactagttg agtcaagaat tatttttcag ttggatcaat caacattcta atatttaatt
     1381 agaaagaagc ttggtcaaca tggataccag gtccaaatgt tgttgtgatc gttacatttt
     1441 tcatgtattg acctttagct gctgatggtt tagcttttag caatacatca tggattgctg
     1501 caaagttttc aactaatttt tcatcgtcaa atgaaacttt tccgattggt acatggatat
     1561 ttccagcttt gtccacacgg taagttactt taccagcttt cacttcgttt actgcttttg
     1621 ttacgtccat tgtaactgta ccagttttag ggtttggcat taagcctttt ggtcctaata
     1681 ctcgtcctag acgacctaca gtagccatca tatcaggtgt tgcaacaact acatcaaagt
     1741 caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag
     1801 ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac
     1861 cagttccgtt tggtaataca acagcaccac ggatttgttg gtctgctttt ttagggtcta
     1921 cgttcaaacg gtatgcaact tcaactgttg catcaaattt tgcaatgttt gtttcttttg
     1981 ctaaagctac tgcttcggca acaggataaa ctttacttga atcaactttt tttaatgcat
     2041 cttgcatttt tttgctcttt ttagccattt tggttcctcc ttgattgtgg ttataacggt
     2101 tgtacctccc acgtgttcta tatctttcaa tatagagcca actgagaagc tgctgtctta
     2161 gggtgtatag attattctac agtgatcccc atgcttcgtg cagttccttc aaccatgcgc
     2221 attgctgctt caacgttagc tgcgtttagg tcttccattt ttaattcagc gatttctctt
     2281 acttgatcgc tagaaacttt cgcaacttta tttttgtttg gttcgcctga acctttttcg
     2341 attttagctg cttttttcaa tagaacagca gctggcggtg tttttgtaac gaatgtgaat
     2401 gaacggtctt cgtatacaga aataacaacc ggaatgatca aacctgcttg atcagctgta
     2461 cgagcgttga attcttttgt gaatcccatg atattgatac ccgcttgacc tagtgcagga
     2521 cctactggtg gagctggagt tgctttacct gcaggaattt gcaatttaac gattttttct
     2581 actttctttg ccacgagaca tacctcctta agtccgtgat gtggttaatt ggagatgcat
     2641 atttctcctc ccacgtttta tctgatctga aaaaatccag acaaataatg tgcaaaacaa
     2701 tgcacactgg tacagtatag caaatttatt tgttttttca agattttttt ctgctatttt
     2761 tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt ttcgttctca
     2821 tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc aaacaataac
     2881 acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg gagaaaatga
     2941 aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt ggtccaagta
     3001 gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata tttggcgaac
     3061 agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat cgggggcacg
     3121 gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat gaacgtttag
     3181 ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt cctaagagtg
     3241 aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt
     3301 cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg aatggattca
     3361 aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat gttccaggta
     3421 tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc actatgacag
     3481 tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat gatcctcaag
     3541 tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta aattatttcg
     3601 attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa gcaaaagctt
     3661 atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc cgttcccgta
     3721 gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg
     3781 gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg
     3841 attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct gtacgcaatg
     3901 ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg
     3961 gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt gatttgacac
     4021 atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca
     4081 acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct gcaagtgcaa
     4141 tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag
     4201 tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca ggcttagttg
     4261 aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc tctgctgata
     4321 tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct gcaatgtacc
     4381 aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc ttagctgtta
     4441 cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga taaagctcct
     4501 taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag aggactaatt
     4561 gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc atcgatcgtt
     4621 tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc aaaaacagat
     4681 gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc aatcgcaact
     4741 aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac accagagtcg
     4801 aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac ttcaaaggac
     4861 gcaggacctc catctagttg acgtccacca tgattggata cccagatacc atctgcccct
     4921 gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat aactggaaga
     4981 tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt tgcagctgca
     5041 taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc gctatacgca
     5101 gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc ttcacggtaa
     5161 ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc tactgctttg
     5221 ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa aaactgcggt
     5281 gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt gctgatggaa
     5341 aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt ttcatgtgcc
     5401 aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat tccgaaaaca
     5461 gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat tttctttgta
     5521 ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc gcgaatatag
     5581 ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc aatgtttaca
     5641 atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc ttctttcatc
     5701 ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat ttactctcag
     5761 atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta cttcttggtt
     5821 ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct ctatttcatt
     5881 tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta ctgttccatc
     5941 actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg ttataggaat
     6001 attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag gcccctttgc
     6061 taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat
     6121 tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg
     6181 tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta tgccgtagtt
     6241 ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact cgttattttt
     6301 attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca aatctcctgt
     6361 tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg cttgctgatt
     6421 tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt agccgtggaa
     6481 aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg ttttttcttt
     6541 atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg caaacaaact
     6601 gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg gttctgtcat
     6661 acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca tatgatcgac
     6721 atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt tctgtactgg
     6781 tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg tcaacttctg
     6841 ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact tcgccaactt
     6901 ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata tgattgactt
     6961 cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca
     7021 caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt tcaacaagta
     7081 cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg acttcagttt
     7141 ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata ctttgcgcac
     7201 gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc aagacatacc
     7261 agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata aaaaaacctc
     7321 agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt gtggctagcg
     7381 agttatttta aaatccagcc aaataagctt tggatggctg tatccattac gtaaaacatc
     7441 aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag ttgttttctg
     7501 cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt catgagacaa
     7561 cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta caatatttac
     7621 aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat tttgtgtagt
     7681 tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt tacttctgcc
     7741 cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt tacttgtcaa
     7801 tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa tttgaaatta
     7861 agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt atataagaaa
     7921 aaagatctga caaccgttac agagactagt acattagaag aagcattaaa aattttagaa
     7981 acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt ccgtggaaac
     8041 atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc
     8101 actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt cttcaaagta
     8161 ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa tcatttttat
     8221 ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag cgtagaacaa
     8281 ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc tgctattgca
     8341 aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat cgaaaaagac
     8401 gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa
     8461 acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga aaatttgcaa
     8521 aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt tcttttttcg
     8581 ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg
     8641 gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa
     8701 atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga
     8761 agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg
     8821 tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac
     8881 caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa
     8941 agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag
     9001 gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta
     9061 cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt
     9121 tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac
     9181 ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta
     9241 aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc
     9301 ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat
     9361 aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga
     9421 agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg
     9481 cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg
     9541 ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat
     9601 tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata
     9661 tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat
     9721 ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa
     9781 aataagcata ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt
     9841 gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc
     9901 gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat
     9961 actttctatc agatggaagt agaattccca gttgcttata ttccagcagg ttctggaaat
    10021 gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta
    10081 gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa
    10141 aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca
    10201 aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta
    10261 ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga
    10321 ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt
    10381 ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc
    10441 gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa
    10501 aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca
    10561 acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc
    10621 acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta
    10681 catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt
    10741 tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta
    10801 cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg
    10861 aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta
    10921 ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag
    10981 tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg
    11041 atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg
    11101 atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag
    11161 gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa
    11221 ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg
    11281 tttcgtttac tgatctcttt gcgattaaac gctagattga tgacgctgaa accgctaaaa
    11341 atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc
    11401 atccgctgac tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt
    11461 gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc
    11521 aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt
    11581 atcttgatcg tcgccatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt
    11641 aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca
    11701 tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc
    11761 gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca
    11821 atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc
    11881 gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct
    11941 ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt
    12001 ggtataaaaa ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa
    12061 gttgacgaga tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg
    12121 ttccgacccc atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct
    12181 atccttttgc accagattca tttttggtat caaattacca accaatgtac ctaacaaaat
    12241 cgcacacaac gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc
    12301 tacttgatag ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt
    12361 aaagcttgct gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa
    12421 gcgaatacca tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat
    12481 cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat
    12541 attacgacct gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg
    12601 cgcgagatca acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa
    12661 aaattgatcc atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag
    12721 cgaaacagat gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc
    12781 attcgcttct acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac
    12841 ttgttctaag actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat
    12901 tccagtatta atcgtttcaa gattgccacc ttgatcaatc tcgccaaagg ccgccataaa
    12961 catcaatcct aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag
    13021 cagtattttt gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc
    13081 tcaaatcctt gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta
    13141 tttttccgta actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat
    13201 ttagttcagt agttgttact tggagctgac cttttttata agcgattcca tcagaagcta
    13261 ttgttcgctg aatcgctgac aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac
    13321 gatccgccgt ccgtaccata tcttttagag attctttggt tcctttagca atcacctgtc
    13381 cttgatccat gatcacgatc tggtcgcaaa gaatttctac ttcttccatg taatgtgttg
    13441 tatagacaat ggttgctcct tgtcgattca gctccttgat actttctaaa attttattcc
    13501 gactttgggg gtctacagct actgtcggct catctaaaaa aattaaccgc ggcttgtggg
    13561 caatcccaca tgcgatgttc aaccgacgtt tcaagccacc actaagttct cctggacgaa
    13621 acgaaacata tttttctaat cccactaatt gaatcacctc ttcaatcaag gctttccttt
    13681 tctctttttc ggaaacatat aaaccgcaaa aataatcgat attttcttgc acacttagtt
    13741 cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat tgccttgaca gcataatttt
    13801 caggagtcat tgttgtcccg aacaatttaa tggttccttt gtcataattt aacaaagaaa
    13861 gcatacagtt gatggcagtc gatttcccac tgccgtttgg acctaacaga cctaagactt
    13921 ctccttcttg tacttgcaga tcaaaatgat tcaatgctac aaaatctcca tagcgtttga
    13981 ctaatttatc aatttcgata atcatcatga aaaactcctt ccaaatttct tgttgatctg
    14041 actataataa agtgcaagaa aagaaactag tgacgattgg ctaaaccaca catgacattt
    14101 gtcacaatga aggagtctgc tatgcataaa tctcggctac tcaacgaaac tagctttcta
    14161 ctggttatct cattgctttt gctggtaagt caaggattcg ttgctatgca agttatctat
    14221 ttattgatcg ccgttttatt cactggtgtc agttatttac tgcctacaaa gccaaattgg
    14281 attttccaag ttattctatt tcttttatgt gcctttttac ctgatcttgt cctgtttctg
    14341 ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc ttgattactt gtttttgtta
    14401 ggttttttgt tgatttcgac ggttggtaat catcccccca tcaaggtcgc ctttgtcgta
    14461 attttgctgt ttttttcctt ttacttacga aaaaaagatc aacgaacgac cgatcttgag
    14521 aaaaagtaca ttgtctccaa agatactcat tgggaaaaac aacaagagtt atcggaacaa
    14581 aataaagcat tggccctgtc acaagaaaca caattagcct tgcaaattgt ggaagaacgg
    14641 aaccgaattg cccgagacat ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta
    14701 caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc attagaaaat
    14761 ttaagtgcca ccgttcacac tgggatggat cggattcgtc aaagtgtcca tgacctccat
    14821 gagacatcga tctcttttca acactcctta gagtttctga tcgctgattt tccttcttct
    14881 gttacgattg aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta
    14941 atggtcatca aagaagcctt aaccaacagt atcaaacata gtcatgccaa aaccgttgtt
    15001 ttacattttc gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga tggcaccagc
    15061 ccacaaaaaa attctgctcc cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa
    15121 caaaagggac aagtccatat tttccaagaa aaccagcaat ttcagttaac gatcatttta
    15181 ccaagggagg gacaccaatg ataacagtag tcattattga tgatgatcca tttgtgacga
    15241 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    15301 gtgccaagga tgcgcgaaac ccatcgtccc gatgttttgc tgacggacat ccgcatgcct
    15361 aaacaaacgg gcatcgatgc tgccaaagaa attttggcta aatttcccaa agccatcctt
    15421 ttgcttttga cgacattcaa agatgaagaa tacatccgcg aagcattttc catcggtgtc
    15481 aaagggtatt taatcaaaca gaacttgcaa gcgattattc cttctgtcaa atccgcttat
    15541 aatggacagg ccgtatttgg taatgaaatc atcgagactt tcactcaaat gatgaagaat
    15601 aaaccgacga ttgatagtca tgcttatgct tcttttagtg aacgtgagct tgcaatcatc
    15661 aaagaagtag ctgccgggaa aaacaataaa gaaattgcag atgccttgta cttgagtgac
    15721 ggcactgttc gcaattacat cagccaatta ctagaaaagc tagatttacg ggatcgcacg
    15781 caattggcta tctattttta tcagcatttg caataagtct tatctaacac aaaaaaacct
    15841 gaatgatgct ccttcgcatc attcaggttt ttttcacatc ttctattcaa ttgtcccgca
    15901 aaccatttcc tcacgattga aagaatcaag gattcgcagg gctgccagga ttcgttggat
    15961 tgttcggcgt gttaggcgga aaaataggcg ccttgtctaa cgttttttct agttttgatt
    16021 cttcacgatc gtctcgttct acatcaggac tttcattcaa tttgaattct ttttccatct
    16081 catctaactt tttaaagtca ttgccagatt cttttgctaa tttcgctaat ttatcttcgg
    16141 tttctttagc cataaagtaa cgcccccctt ttgatttcag cttagcatat cacactgaaa
    16201 aaacaaaaga aaacgctaac caacgatcat ttttagctat ttctcatctt taaaatcaag
    16261 cattttttgc cggtataaaa tcgtagggag aactgttttc cataccaatc atcggatcta
    16321 ctaactggtt atcgatcact tctgctgtaa gaacgaaatc tttagaaaca gcaggttcta
    16381 cctcttcttt cttttcttct ccaacaaatg aagaaaacag atctggttcc actgtttcca
    16441 ttacttggtt tttcgtttca ttcgtcccac tgtcatcttc tacaggtagc ttttctttta
    16501 caacccgtgg ctcttttgta tagttcccat cttcaaatgg atcatttccg accaaagaat
    16561 cttcataagc tgctagtttg gtccggagag tgatattcat tttatcatta tccatgatcc
    16621 ttgatttcaa cgtagtcatc cggcttgctg attcattcgc tacacaagtg acataggctt
    16681 gctcttcccc taacaaaatc aacagttcct tactgcgagt cactgccgtg tagagcaaat
    16741 tacgctgaag catccgctga tattgatgga ccatgggcaa aataaccatc ttgaattcac
    16801 tgccttgtga tttatggatt gagcaacaat aagaaagagt gattttattc cattcgtttc
    16861 ttttataagt gacctcattt gcatcaaact ggataactaa ttcatccact ttatcttcag
    16921 aatcttttgc caagatgata cccgtgattt ctcccatatc tccattgaat acattcaatt
    16981 cgggagtatt gactaattgg aggactttgt ctcctatccg ataaacggtg tcgttccaat
    17041 gtacttcctt ttttccaggt ttccctggat tgaatatctc ctgcatcatt ttatttaacg
    17101 catcgatccc tgcaggtccc cgatacattg gtgctaatac ttggatatct tgtgcacgga
    17161 atcccttatt tttcgctttt tccacaattt tagagatcaa gggttcaatt tgataagcat
    17221 cacttcggaa ataggaacga tctttttgat tctttgtgaa atcttttggc agacgtcctt
    17281 ctttgatctc atgggcaaga ggaataatac tagaaccatc accttgccga taaatctgat
    17341 tgagttccat tttgggaatt tgttcgattt ccaaaagatc atggagtact tgaccaggac
    17401 cgacagaggg aagctgatct ttgtctccta ctaagataac ttgcatattc gtcggaatcg
    17461 ctttgaataa tgtgttagcc agccatgtat cgaccataga catctcatca acaatcaaaa
    17521 gtccgccttc taattctttc gtactgactg aaggtgtttt ttcccgtcca ttcagcccca
    17581 gaagacgatg gatcgtactg gcaggcagac ctgtcgtttc gttcattcgt ttagctgctc
    17641 ggccagttgg tgctgcaagt agaataggaa aaatttcttg ggtatatttt gaaggatcca
    17701 attccagtcc gttcaactct gcaaaaagat tgacgattcc gttgatcaca gtagttttcc
    17761 ccgtacctgg tcctcctgtt aaaataaata gaggggaacg gattgcttca gcgatagctt
    17821 gttcttgcga aggtccatac tgtatgccga atattttttc gatacgccga atattcttct
    17881 gcacttcttt ttcaggataa tggatttctt ttttacgact gagaaggcgc tggatcgaat
    17941 taccgatccc ccattcagaa aagtacaaac tgttttcgta aagattagtc tcttcttgtt
    18001 gtatctttcc ttcttcaaca agttggataa ttacagtagc aacctgatca ggctggattt
    18061 caattgggcg acttgtttct aaaatacgga tcgtttgctg aagcaatgtc tccgccggta
    18121 cgtatgtatc tcctgtttga actgcatgct gaaaaatctg atgcaagata gctgctcgaa
    18181 ttcttctcgg tgaatctgct gcaataccga tttgttcggc tagattatct gcacgtttga
    18241 acccgattcc ttctatgtct tcgactagct ggtacggatt ttcttcgatg atatccaaag
    18301 actcgttctt ataggcctgg tagatactga aagctaactg actaccgaac ccgtaacgat
    18361 tcagtcccat gatcacttga tccatcccgt ggtttagccg aatcgtctca gcaagcattt
    18421 ctctttttgg cttcgtcaaa ccggagattt gttttaatac cgaaggatca gcaatgattt
    18481 tttcgatagc atcttctccc agcaattcta caattttttc tgctgttttc ttcccgattc
    18541 ctgggaattt ttcgctggaa agatacgcta cgatcccatt tgcagttgtt ggcttttctt
    18601 gctcatatct ttcgagttta agctgccttc cgtatttagg atgatccact aattctccat
    18661 aaaaacgata agtctcattt tcttgaatat cgccgaaact tccggtgact acaatctctt
    18721 tttcacggta atcagctgtg gtttcactga tgtgtaccaa aagcactttg taaaaattac
    18781 taggattttg aaaaaaaatc gcggcaactg taccaacgaa ataaggcatt tcttctggct
    18841 gcatcggaag ccgcctcctt cttctatagt aaaggttctg gtgctgtttc agttcctaaa
    18901 aaatctgttt ggttccatac tttcagttca tatggcaata atctgcaagg ctcttcagtc
    18961 tctaaaatcg tcaaactact attgaacaat ccacccattt cacgcaactc acttagctct
    19021 tttcctgtca tccattgaat ggctgcagtt aaagaagctc catgtcctac gaataaaagt
    19081 gggccatctt tatgtcgggc aaccgcatct gctacaacag tttcgatacg tgtgattgcc
    19141 gattcaatcg tttctccgtg aaaaggagaa ggatcataca gatctaaatg attacgcaaa
    19201 ttcggcagat tatccggata tttctggtac atttcatcga tcgactgacc ttctaaagta
    19261 cccagcccta gttctcttag cttatctgta taaaaaatct ctaccggacg agcaagctct
    19321 ttgttgatac cttctgctgt atcacgtgct cgctttgctg tactggaata aattgcttta
    19381 aaaggaacat attttacctt tttcccaaat gctttgattt cttcgtaact ttgtggcaaa
    19441 agcggagaat cgccattgct cccttgaaaa cgtctttcct gattccattg tgtcttccca
    19501 tgtcgggtaa aatatagttc catacgcagc aaactcctgt ctttctttta ttttactcta
    19561 taataaactg tctaatccag cttgtcattt tcatagtgat gcgtcaattc taaattaggg
    19621 aatccttgct ggcgaagtgc ttcatacact atcattgcag ctgtattcga taaattcaat
    19681 gagcggacat gctcatcatt catagggatg cgtaaacact tttcttcgtt ttcacgcata
    19741 aatgtttctg gcaaaccggt tgtttctttt ccaaataaaa agaagtgctc ttctccatca
    19801 gcaaaatttt cgtcactata tactttatgg gcaaattttg agatcaaatg tagtttacga
    19861 ttacctaaaa atgttaaaaa agcttctaaa ttactgtggt aagtaatatt tacatcattc
    19921 caataatcca accctgcccg ttttagatgt ttgtcatcag ttgaaaaacc tagtggctcg
    19981 attaaatgaa gcggagtatt cgtggctgca cacgtacggg caatatttcc agtgtttgca
    20041 gggattaatg gttcaaataa aacgatatga ttcatcagga tcattcctct catatttatg
    20101 ttcgtttcta ttttctgaca atttgatttt tgcataaaaa aaaacgtatc aactcggtgt
    20161 cagtcactgc gagtcgttac gttagtgaag ctcttctcac taacttttat cagaacagga
    20221 acctgataaa aaccaatgaa aaaacaactt gttgctaata taacaccctc aacttttttt
    20281 tgcaacctct actcattaaa aactgctaga aaaacgtttt ctcttagttc cttttaataa
    20341 ttctatgtca attgtaatct tcttcaaagg attcttatat aagtctgctt tgattcgctc
    20401 ttctacctgc cattctaaag gtgacatggc caaaagatct ttttgatttt cctctggtat
    20461 ctcaaattca taggttacac gctgacgttc agttaatttc attgcttcag caaacttggc
    20521 tactacacgt tcattggaat aactctgttt ttcaggttga ttcggataaa aagcctgacg
    20581 tagttccttt aaataatttt cttgtggcac caccttcaac acttctccat cttctgagag
    20641 tacacgttgg aattcttggt aatgagaagg cgaaaagata tttaaaaccg cgttgaatgt
    20701 ttgatcagca aacggcaaat tggtcaaatc agctacacac caaaatgctt cggcagattg
    20761 ttctgtagct gcgtagactc cttcttttga aatatcaaat ccaacagcat tttgaatctt
    20821 tttcaatttt gaaagttcat tcaagaagct accttctccg cagccaacat ctaatagcga
    20881 gacttcttca ggtaaatgct gcgcaatagt ctcaataacg ggctgataca tgccagattg
    20941 gatcattcta cgccttggaa cgaacattcc ttgatcgtat tctgttttaa ccgcatggga
    21001 taaaaaataa agtgttcctt ttttggaaag gtcaaaacgg tgattatttt tgcatatcaa
    21061 gccattatca gactttttta cttcttcttg gcaaaccgga cagcggaaca gctgatgatt
    21121 ttctgccaaa aactggcgtc ctcggtcaat tttttttaac atagttcccc ctcatttctg
    21181 ttttatctta ccgttagatg ctcactttgg caactgctaa aaatatgata caatgcctag
    21241 aagaaagtga aagataaagg agaagattag atgatcaaag aattttgcgc tgaaaactac
    21301 acatctattc ctctagcaat tgctagagga gccgatcgga tcgaattgtg tgacaactta
    21361 gcggtcggtg gcacaacccc gagtactggt gtgatcgaag aaacgttaag ttatgctggg
    21421 gaaaaagata tccccgtaat gacgattatt cgtcctagag ggggcgactt cgtctataac
    21481 gatatcgaat tgaaaatcat gcatactgat ttgattgaag caaagaaatt agggacggat
    21541 ggcgtagtat ttggttgtct tactccttca ggctggctag acgaagaggc cttagaacta
    21601 ttgatcgaaa cagcagaagg gttgcagatc accttccata tggcttttga cagtattcct
    21661 gaagatcggc aattcgaagc aatcgattgg ctggtagaac atggtgttga ccgtatcttg
    21721 actcatggcg gtcctgctga caaaactatt gtagaaaatt tcccacggct taaagaattg
    21781 attgcctata cagatggccg cttaatcatt ttgcctggtg gtggtgtcac tgcagagaat
    21841 gcagcacatg tagctaaaga attgaacgta agcgaagtac acggaaccaa aatcgtggat
    21901 cttcagttat aatcttataa acaaaaaaac atttgcaaca ggcataatgc ttattgcaaa
    21961 tgtttttgtt tttttacata aaatagccca taaataaaga agcaatattg aaataaatca
    22021 gtacactcgt caaatcgctc aaagtcgtga taaagggacc gcttgctact gctggatcaa
    22081 atcctagacg atccattagc atcgggatta aactacctgc aagattcgcc actgtgattg
    22141 caaatagcat agccatccct atgacaaacc ccaagataaa attatgtttc caaaaaccta
    22201 cgattagaaa aatcgtgatc cccgtaacag ctcctgtgac aacgccggtc aagacttcag
    22261 ccaaaacaag tcgaacaaat ccatgatctt tctcatcaga aatcgcaagt cgacgaacag
    22321 ccacagctag cgactgggtt ccagcattac cggcagttcc tgtaatcagc gaaatgaaga
    22381 ctgctaaaat actcgcttca cttaccaagt cttcatagtg actgattaaa gaagcggtcg
    22441 acatacctaa aaacaacaga gtgatcagcc aaggcaaacg tcgagacgct gcctttactg
    22501 gattttcatt aatttcttcc acattgaccc cggcaagtcc tgaataatcg ctagcagctt
    22561 catcatcaat aacgtcgatg atatcatcga ctgtgacgat tcctaacaaa tgatcatcat
    22621 aatcggtaac aggaagagct aagaagtcat aatctctaaa tgtttgtgcc acatcttctt
    22681 ggtcgtcgcc tacatgaact gaaagcactc gttcgctcat cagatcagaa atcattgcgt
    22741 cgtcatcatt cacgattagg tcgcgcagag aaataacccc tactagctga ccttcttgat
    22801 taacgacata gatgtaatag attgtttcag ctacatctgc ttcgttcttc agcacgtaca
    22861 tcgcagagcg aacagtttga ttggctacga tcgaaacaaa ttcagttgtc atgatcgcgc
    22921 cagccgtttc atcctcgtaa tggagaagtt ccttgatttc actggcatcg tctgccgaca
    22981 tcaaacttaa atatttagca atttgctttt tatccagcgt attaagcaag tcaacagcat
    23041 tatctgtata catctcagaa agcatatcag ctgcatagct aggacgcatt tcatctaggt
    23101 attctttcat atgttcgttg tcttcttcaa tcacatcgaa catatccgct aattctttag
    23161 gcgacagata ggaatagatc agctgcctgt tctcttctgt aagagattgg tagaattgcc
    23221 cctgctcata gatatgcatc tctaaaaaac tgtttctgaa tgcctgaata ttattttccc
    23281 ttaaatcttg agtcaactgg gcaaatcttt cctctaattc ttgattttca tccaaaccaa
    23341 tctcacctcg ccttattctt caaaccgtct cacaactgcc tccatgtctg cggctaaagg
    23401 ctgcttgaac tccagcaact cttctgtaaa tggatggtaa aaacgcagat cataacaatg
    23461 aagggcttgt ctttttattc ccagatccat tcgcccgcca tacatatcat caccgagcag
    23521 ggcacagcca attgcggaaa aatgaactct tatttgatgt gtccttcctg tatgcagctg
    23581 gatatcaact aacgcgattt ctgttagtcg cttctctatc cagtactcag tctgtgccga
    23641 acgtccttca tcagagacca ttcgcttcaa tagcgaagaa taatctcggg cgatcggcaa
    23701 atcgatatgt ccatgtgccg ataaatctga aagccttcca ctgactaagg cctgatattt
    23761 tttcacaaac ttcttttcac gaagttgcat atctaatttt gcgtgcgcaa atccatgctt
    23821 tgcaaaaagc atcaagccag atgtatcccg atctagtcgc gtcaccacat ggattacttg
    23881 gttttcgtaa ccttgttttt tataataagc ctttacacga ttagccattg tcccatttgg
    23941 atggtattgg gcaggaatag acgaaacgcc tgctggttta tttactacca gaacgtgttc
    24001 atcttcatac acgatatcga tcggtgtttc atccaaaagt acggtttcat gttctccttc
    24061 agcaggaatc acaatggtta ccttatcatc cttagctaga gaaaacaaga cattttctac
    24121 ttgatcatta actttgattt gaccgccttg aaattttatt ttcgctaaca gaccctttga
    24181 gatccctttt tcttttaaaa aatattttac ttgctgagaa tgatctttct cataatccca
    24241 tgtaaactgc atatcaatcc tcactaataa aggcatcttt cacacgatgc caaaaatgca
    24301 tatgtcgata agaagcaaaa tggatccgtt cttctgctat tcgataataa attgagcgga
    24361 tatttgcttt tgcgacattc aattgatcaa ccgttacaag ataatcttct gtattttcca
    24421 attttatttc tagccattca tcatgggcaa tcacgatcgg agaacccaaa gtacgaaaca
    24481 ctcgattatt cagtgaagca atctctgcca actgaaaggc attgatacta ggatggatca
    24541 cagcaccgcc gacactttta ttatatgctg tcgaaccagt aggcgtagaa atcgtcaatc
    24601 catctcctct aaaactttca aacagctcgt ttttgatgta tacatcggct accatcgtac
    24661 gattggcccg tttaatcgtt gattcattca gtgctaaaaa atgctggtcc ggtgtttcat
    24721 ccaaataact gatccgaaca tccagcaagg gataactaac actttgttcc cggttcgttc
    24781 gtaagctgtc tactagttct tccagctcgt aatcccgcca atccgtgtaa aaccctaaat
    24841 gacctgtatg cacgcccaag aaacgcacat cattcagaca atgactgtag tgatggaacg
    24901 ccgacaataa tgtgccgtct ccgccgactg agatgaccaa ttctggctca tgatcatctc
    24961 gttgaatccc tgcttgttct aaaagtgcgt tcaaacgttc tgtcacttct atagtctttg
    25021 cttctttatt atgaacgatc gctactttca tcccctgcct cctctctttc gctttctttc
    25081 ttcaaaagcg ttcgatagta gacatcatct gattttcctc ttccatggga gaataaccgc
    25141 tgtgcttctt ggatttcttc acggatactg gacatctctt catccaactg ataggcagct
    25201 tctgccgcac gctgaagtcg ttcactcatt tcttctggaa aaacgccttg atatttataa
    25261 ttcagcgaat gctcaatcgt tgcccagaaa ttcatcgcaa gagtccgaat ctggatttcc
    25321 gccaaaattt tcttctcacc gctgatcagc tgtacaggat actcgatcac cagatgataa
    25381 gaacggtagc cacttgcttt tttatttgtg atgtaatctc gttcttggag aactttcatg
    25441 tcttttcttt tacggatgat ctcaacaact tgatggatat cttctacaaa ctgacacatg
    25501 atccttaacc cggcaatatc agacatttct tcttctaaac gtcccattgg aatatgccgt
    25561 aattgtgatt ttgttacaat actatcgact ggcttgactc tcccagtgac aaattcaatc
    25621 gggacatgtc gattttgttc acggaactgt ttgcggatcc cccgcagttt cactttcaat
    25681 tctgatacag tttgttcata tggtgctaaa aatgcttccc aatctcgttc catagctgat
    25741 acctctactt cattaacatt cattgtttat tctaccatat atgcaaagtt ttttcgtact
    25801 cttgatttat gcattgacca aaaaaagaaa attaggcaaa atagacaaaa aggagtcgat
    25861 caaatgacac aaatgcttga aatcgaatat aaatctatgc tgacaaagca agagtacgag
    25921 atattcatcg ctcactatca attaacagat aaagatttcc gtgtacagac taatatttat
    25981 ttcgataccg ttgatgaaca gctgaagaaa caaaattgtg gcctacggat ccgtttcgtt
    26041 ggtcatcaag ctgaatatac attaaaaaca cctgctgaaa aagggcgtct cgaaacaacg
    26101 gataccttta taacagaaga agcagaagca tttatcttcg aaaaacgtct gcctcgttca
    26161 ggcgcagttt ttcaaaaact ttctgatttg aatatcgatc ctgcttcatt aatccaaacc
    26221 ggaaagctta caacaaaacg agctgaattc cctattgaag aaggactttt agctattgat
    26281 gaaagctggg gagacaatct acacgatttt gaactggaac tagaggtagg ggatgcatct
    26341 gtgggaaaaa ttgcttttat aaatttttta aaacgttttt ctttacctta tcgtcctgct
    26401 aagaacaaga tccaacgtat gcttgaagct aaaaattaga aacctttcat tttcacaagg
    26461 cacttgagtt cattttttta cttttttctg ttaagttata ggatgtgaaa gaatctagtc
    26521 actagttgta tagaatctat tttgggggag attcatatga tcgaaatcta tttatttgtt
    26581 aatcctttag gggaatactg tttcgaaatg gaacaacagt tattacaatt tatcgaagaa
    26641 gagtacggga caccatcaaa agaaaagatg caatttcgct ttcttccttt ggtcaatttg
    26701 cagacaattg gggatgtcat gcaacaaaaa ggaatttctc agaacaatct tgaggaacgt
    26761 aatcacttat tctcaatgac ttattctgca gcattggact gcaaagcggc gcagtttcaa
    26821 ggaaagaaaa aaggccgccg ttttttgatc aaattgcaag aagcagtcgg ctgtaatgac
    26881 ataccatatt ctcaagaact tgctgaatcc atatttgaag aaatcggcgg cgatttgaat
    26941 atgtttaaag aagatcgtca gtctgacttc gttaaagaag tattcttttc tgatcaaaaa
    27001 attgctcggg aaatgggcat cacaaaacac ccttctgcag tcgtttacaa ttatgcctgc
    27061 gaccgtgatt ttggtgtttt agtagaagta aaaggtacaa gtagcctttg gagaatcaca
    27121 gagctgtgta aaacaacaga taactcatat caagttttta atggtgaaca tttacataca
    27181 aatgattctc agttccattc aagaactagc catttacact tgctttctaa ttaa
//
LOCUS       DACCXW010000027        26644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCXW010000027 DACCXW010000000
VERSION     DACCXW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.027"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1297)
                     /locus_tag="IVY71_000942"
     CDS             complement(<1..1297)
                     /locus_tag="IVY71_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7985009.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHD"
     gene            complement(1299..1967)
                     /locus_tag="IVY71_000943"
     CDS             complement(1299..1967)
                     /locus_tag="IVY71_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7985010.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2097..2681)
                     /locus_tag="IVY71_000944"
     CDS             complement(2097..2681)
                     /locus_tag="IVY71_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7985011.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3012..3245)
                     /locus_tag="IVY71_000945"
     CDS             complement(3012..3245)
                     /locus_tag="IVY71_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP7985012.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3260..4210)
                     /locus_tag="IVY71_000946"
     CDS             complement(3260..4210)
                     /locus_tag="IVY71_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7985013.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4207..5049)
                     /locus_tag="IVY71_000947"
     CDS             complement(4207..5049)
                     /locus_tag="IVY71_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7985014.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5051..5770)
                     /locus_tag="IVY71_000948"
     CDS             complement(5051..5770)
                     /locus_tag="IVY71_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7985015.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6462..6620
                     /locus_tag="IVY71_000949"
     CDS             6462..6620
                     /locus_tag="IVY71_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7985016.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            6692..7918
                     /locus_tag="IVY71_000950"
     CDS             6692..7918
                     /locus_tag="IVY71_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7985017.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8006..8398)
                     /gene="rpsI"
                     /locus_tag="IVY71_000951"
     CDS             complement(8006..8398)
                     /gene="rpsI"
                     /locus_tag="IVY71_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7985018.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8414..8857)
                     /gene="rplM"
                     /locus_tag="IVY71_000952"
     CDS             complement(8414..8857)
                     /gene="rplM"
                     /locus_tag="IVY71_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7985019.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9376..9843)
                     /locus_tag="IVY71_000953"
     CDS             complement(9376..9843)
                     /locus_tag="IVY71_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7985020.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9963..11501)
                     /locus_tag="IVY71_000954"
     CDS             complement(9963..11501)
                     /locus_tag="IVY71_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7985021.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11581..12267
                     /locus_tag="IVY71_000955"
     CDS             11581..12267
                     /locus_tag="IVY71_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7985022.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12468..12995)
                     /gene="msrA"
                     /locus_tag="IVY71_000956"
     CDS             complement(12468..12995)
                     /gene="msrA"
                     /locus_tag="IVY71_000956"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7985023.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13079..13756)
                     /gene="pgmB"
                     /locus_tag="IVY71_000957"
     CDS             complement(13079..13756)
                     /gene="pgmB"
                     /locus_tag="IVY71_000957"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7985024.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13753..14883)
                     /locus_tag="IVY71_000958"
     CDS             complement(13753..14883)
                     /locus_tag="IVY71_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7985025.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14883..17066)
                     /locus_tag="IVY71_000959"
     CDS             complement(14883..17066)
                     /locus_tag="IVY71_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7985026.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17063..18088)
                     /locus_tag="IVY71_000960"
     CDS             complement(17063..18088)
                     /locus_tag="IVY71_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7985027.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18133..18960)
                     /locus_tag="IVY71_000961"
     CDS             complement(18133..18960)
                     /locus_tag="IVY71_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7985028.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18957..20024)
                     /locus_tag="IVY71_000962"
     CDS             complement(18957..20024)
                     /locus_tag="IVY71_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7985029.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20050..20886)
                     /locus_tag="IVY71_000963"
     CDS             complement(20050..20886)
                     /locus_tag="IVY71_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7985030.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20883..21752)
                     /locus_tag="IVY71_000964"
     CDS             complement(20883..21752)
                     /locus_tag="IVY71_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7985031.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21822..23105)
                     /locus_tag="IVY71_000965"
     CDS             complement(21822..23105)
                     /locus_tag="IVY71_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7985032.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23119..24804)
                     /locus_tag="IVY71_000966"
     CDS             complement(23119..24804)
                     /locus_tag="IVY71_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7985033.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25079..26104
                     /locus_tag="IVY71_000967"
     CDS             25079..26104
                     /locus_tag="IVY71_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7985034.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         7991 a         5379 c         4451 g         8823 t
ORIGIN      
        1 gatcatgagt agctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa
       61 tcctttccat attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag
      121 tttttgacaa tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa
      181 acggtaattg atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat
      241 ctttactaac tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt
      301 ggtaatccat ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta
      361 tctttccttt ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca
      421 caccgttttt ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt
      481 ttagaatatt ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga
      541 tatagggaac ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt
      601 cagtttttaa caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt
      661 gatcaattaa ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt
      721 tattttttaa atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg
      781 aagacggttc atccaatata attaatttag agtttagcga taaactacat ccaatagaaa
      841 ttttctgccg ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat
      901 ctaataattc agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt
      961 tttccatcga aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat
     1021 cttgacttac gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt
     1081 tattttcaaa taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat
     1141 tcattaactt taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac
     1201 tgcctttttt tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg
     1261 aaaaagaaac attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa
     1321 aaaatagtaa cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa
     1381 gaacttcttt tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca
     1441 gcaatcatag ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc
     1501 tgaaaaaaag acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca
     1561 gaacgataag tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt
     1621 aaatgtattt tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac
     1681 catgagatca ttacgaccgg caacattcga aatacaatat ataaggatgt cgcaaaaaat
     1741 atagtaaaaa actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa
     1801 taaatcaaga tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta
     1861 gcaataatga aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga
     1921 aaaaaaatat ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca
     1981 aaaaataaga tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag
     2041 tgcgatctta tcaaaaagca taactgagaa caattctcaa tgcaagcttt ttttaattaa
     2101 ctcagtaatt caatccaccc aggaacagaa aattgataca atcttctggt taatagcata
     2161 gtcttttctt ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa
     2221 atgtagaagt ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc
     2281 tttaacttat ctagacaaca gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca
     2341 atatcttgat atttggttcc agctgattgg aataaaatca aatcgaatat acgtttattc
     2401 tcaaaaatat aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct
     2461 atatctatat tttctaagtt taagaggtat ttggtaaact cttcattata catattaata
     2521 agcgcattaa tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt
     2581 ttataaaaag cacccgtagt tacgcctgcg gtcaggcaca gttgccttat cttagtttgc
     2641 tgaaatcctt ctttttcaaa atggcttagt ccactttcta ttaacttttg gtgagttata
     2701 atataatctc tggtttctat gggtacttcc tcctaataag atgaattaac tttattatct
     2761 tacacaggac ttgaagtttc aatcgttaac gtttattact gaactcattc cttagagcta
     2821 aactaatgag aaagagcaga ataactgata caaaaagcaa tcctacaaga attaatagac
     2881 tcatctaaat cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc
     2941 agctattagg ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa
     3001 aataaagtct tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca
     3061 gttaattgaa atcctatatc taaatagtcc tcatatctaa gtttagccag catctcactt
     3121 acagatgctg gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt
     3181 ctttttgata attcctgaat tgctagtaaa taaattagta tagtagtcgt tttaatttgt
     3241 accataccta ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt
     3301 tcatcatcgc atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa
     3361 tcgttgaata gataggaata ttggtatctt ttgatactgt tttcattggt ctatcatcca
     3421 cactgctttc tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt
     3481 ttatttgatc tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca
     3541 cattatacgc tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat
     3601 cattcggaat tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa
     3661 tatacttatc tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact
     3721 gtttttcaat atttttagcg taaataatac cgttttctaa atttaaccaa gcatgaggat
     3781 cttccttccc tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg
     3841 caaaatagtc tttgttttcc tctttgttcg catttttaac taattttgta aaccaagcat
     3901 ttcctccagt ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt
     3961 gaacatcttc aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt
     4021 gtaaatcaat tttatctttt gctatatttt cggtaatatc tgctaaaatc gaattagtaa
     4081 ctactacttt taatttttcg ttcgaagcca ctttatcttt gtctttacta tttattgaac
     4141 atgccccgaa tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt
     4201 ttctcatcat aacgatctct ccttctttct taaaaatttt tgttttggtg agattagaaa
     4261 actaattaca aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa
     4321 actataacca ataaacaatc caagcaatga tgaacttgct ccgactgttg cagaaataaa
     4381 tatcatagtt ttcaagctat ttgcatataa atatgctgta gctgctggtg taattaacat
     4441 tgcaacaatt aatatagttc ccacactttg catagctgtt actgctacta aagttaatag
     4501 aaccatcaat agataatgat aaaaactgac attcatccct attgcttttg ccattaatgg
     4561 atcaaaagaa gtgattaata attctttaaa aaatagaact actataacca acactaaaac
     4621 cgaaacgcca attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa
     4681 aatatgaaat aaatccgttg agctatttgc aactcctata aggataactc ctagtgctaa
     4741 aaaagaactg aaagtaatac caattgctgt atctcctttt attacactat tgctttttat
     4801 aaaagtaata atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat
     4861 acctaaaata taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat
     4921 aagagacatt cctcttaaaa taataaaaca acctactgtt ccagaaacaa tccctataac
     4981 tacagatgta attagagcat tctgtaaaaa gtgaaagtca tacagaccat taataaaatt
     5041 ttgaagcata ttattccccc tctccaataa atatagtatc tccataggct ttttttaaat
     5101 tttcttcatt aaatacagat tctacacttc cgtgagcaat tagatttcga ttcaaaagaa
     5161 taatattatc aaaatacttc tttaccttgc ttaaatcatg atgcacaatt agcactgttt
     5221 ttccctgatg tttcaatttt tttaacgtat ccataattat tcgttcacta actaagtcta
     5281 tccctacaaa tggttcatct aaaaatatga aatccgcatt ttgagcaaga catcttgcta
     5341 aaagtacacg ttgaaattgt cctccagata attctcctat ttgatgatta gaatattttc
     5401 ccatatcgac cttttccaaa gcctttgaaa ctctttgcca ttctgcattt ttaattcttt
     5461 gaaaaacttt cattccggca tatgttccca tagatacaca ttcttttact tttattggaa
     5521 aagtaaaatc aatatcagct ttttgctcta catatgcaat cttcaccagt tttttattga
     5581 tatcttcatt attaatggag acctctccct gatgagggat aatatttaac attgctttaa
     5641 gcaatgtgga ttttccagca ccatttggac caataatacc tgttaaagca ccttcttgta
     5701 tttttaaaga aatctcgttc aatgctaaaa aatcgttata acaaacatta agctttttca
     5761 cttctaacat aactttctcc ttctaaatga taatgattat cacgatcata tggtatacaa
     5821 aaattatatt ttagtcaacc ctaaatttta tagtttagat ttttttctgt tgctgaagat
     5881 tagaatgaca aaactatttg aaactacgcg tatagtaagt agctttcaaa atatataaaa
     5941 aaggagcgtt ctcaaatgag aacatgctcc taaaactact ttattggcgt atggaaaaat
     6001 tatacttgaa gtggatagcg ggttgaataa gttgataagc atcaaaacat ttataaaaat
     6061 tattcaagac tatgtctgtt ccatatacaa atatgaaaat cagtaaactc catttaactt
     6121 ttttcgtagt gtgtcttttt atacatgttt atcgttatta atagtgaata ggaaaaaaac
     6181 tatttttgat cattgacaat taaatacaat ttttgaataa cgatccctgc tattcgagct
     6241 agaaactcaa gtacgccatg acctgattag cgacaaatac ttagagattt ctttttgttc
     6301 tgggaaaaca aaatgccatg acaatagcag caaataatga tacttccgta aggctcgccg
     6361 taaactgggg agaggtgaaa ttcctatgtg cttacccaaa gcaattcaaa aatttcataa
     6421 ctactatttt aggagatgat tttatagaat ccagagtcta cctgaataaa aaacaaacct
     6481 gtgcatttct aggaatcgat aataatactc tagataagtg gattcgtata tataatttcc
     6541 cagtaataag agttggtggt gtagttcgct ttgacgtaga agatattaaa gactttatga
     6601 atacatttaa aataacttga aattttcttc tcgccgcata tactaaaatt agtgaaatat
     6661 actgccttac tttttttaag gagtgaggat tttgcctaca aagctttcaa atgggaaata
     6721 taaaactaat ctgagatatc caaaaaggtt taaagaaata acaggaattg catcagaaaa
     6781 gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa gccgaaaatg atatgaagaa
     6841 gaaaatagaa aaagttttga gagaggagaa tgcaaactca ttagaattaa aaggaaaaat
     6901 aacgtttaaa aaattttacg aatctaaatg gcttcctaga tatgaattag gtcaaacaat
     6961 tagatcaaac aggcctccta gtgatataac cattagtaat acaaaagata tatttcggtt
     7021 gcatatttta cctatgtttg gtgaatacgc aatgaattat ctaaatatca atactgagat
     7081 aatatcagat gaattaacca aaaaatcgaa agaatatgca aatataaaaa tcataaaagg
     7141 atatgttcgt tccatgttcg atattgcaga aatactaaat tatatcgaat ttaatcgaac
     7201 tactaaaatt attcaaagta tcactgcacc taaaaaaaat gctttagaag aaaaaagaat
     7261 acaagaaggt aagcaagcac tatcatcaaa agaacttact aattggatag aagcagtaaa
     7321 tgatgatttt aataatcatt tacttacttt tcatgattac acacttttta tgattaccct
     7381 gtatcttggt gatagaaaaa gtgagactta cgctttgcaa tggaaatata ttgattttga
     7441 aaaacaaacg gtccgtctta aacatacttt agataaatat caaagaaaaa agtttacaaa
     7501 aggtcgaaaa gataccgtca ttcaagtacc agaagttgta atgacattgc taagtgaatg
     7561 gaaatcggta caagctgatc aattgttgaa attaaaaatt aagcaaacgt tagatcagta
     7621 tctctttact tatactaaac catctggaga agtaaattgt cctgttcatg ccgattacct
     7681 aaactatcga atcaattcaa ttaaaagaag gcatcctgac ttagttcatt tatctccaca
     7741 caaactccgt catacttatg caactatagc tagacaaggt ggagcagata tgaatcaaat
     7801 ttcaaatgct ttaacacact cagatatttc tacaacaaag atatatgtga acacaccaga
     7861 tatagttgat aaggctgttt ttgaagcttt tcagagagga ttgaataagt gtgattgata
     7921 tgtgttctta aatgtgttct taaaaacaaa agaagcactt tccaaataat tgaaaagtgc
     7981 cagaaacgtt gatatataaa ggatattaac gttttgagaa ttgtgaagct ttacgtgctt
     8041 tcttaagacc tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac
     8101 gtttaagagc taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac
     8161 ggatagctcc tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg
     8221 cgccttttgt ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg
     8281 gaatgtattc ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac
     8341 gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg
     8401 aaattccctc ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat
     8461 gtggatgttc tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt
     8521 ttttaggaag catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca
     8581 attcgccagc agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt
     8641 tgtcagtcgc ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat
     8701 cgccagtatc caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa
     8761 caactgttga aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt
     8821 ctacttcgcc tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt
     8881 ctgatgtttg acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt
     8941 aacatattac ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat
     9001 atgaaacttt acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa
     9061 aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt
     9121 ctcctgccaa ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt
     9181 aaaattgttt cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg
     9241 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg
     9301 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
     9361 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
     9421 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
     9481 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
     9541 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
     9601 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
     9661 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
     9721 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
     9781 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
     9841 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
     9901 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
     9961 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
    10021 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
    10081 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
    10141 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
    10201 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
    10261 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
    10321 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
    10381 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
    10441 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
    10501 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
    10561 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
    10621 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
    10681 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
    10741 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
    10801 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
    10861 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
    10921 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
    10981 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
    11041 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
    11101 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
    11161 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
    11221 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
    11281 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
    11341 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
    11401 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
    11461 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
    11521 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
    11581 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
    11641 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
    11701 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
    11761 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
    11821 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
    11881 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
    11941 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
    12001 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
    12061 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
    12121 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
    12181 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
    12241 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
    12301 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
    12361 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
    12421 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
    12481 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
    12541 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
    12601 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
    12661 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
    12721 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
    12781 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
    12841 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
    12901 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
    12961 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
    13021 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
    13081 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
    13141 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
    13201 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
    13261 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
    13321 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
    13381 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
    13441 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
    13501 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
    13561 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
    13621 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
    13681 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
    13741 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
    13801 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
    13861 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
    13921 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
    13981 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
    14041 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
    14101 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
    14161 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
    14221 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
    14281 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
    14341 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
    14401 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
    14461 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
    14521 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
    14581 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
    14641 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
    14701 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
    14761 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
    14821 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
    14881 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
    14941 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
    15001 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
    15061 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
    15121 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
    15181 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
    15241 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
    15301 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
    15361 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
    15421 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
    15481 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
    15541 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
    15601 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
    15661 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
    15721 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
    15781 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
    15841 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
    15901 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
    15961 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
    16021 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
    16081 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
    16141 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
    16201 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
    16261 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
    16321 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
    16381 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
    16441 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
    16501 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
    16561 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
    16621 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
    16681 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
    16741 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
    16801 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
    16861 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
    16921 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
    16981 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
    17041 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
    17101 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
    17161 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
    17221 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
    17281 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
    17341 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
    17401 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
    17461 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
    17521 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
    17581 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
    17641 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
    17701 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
    17761 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
    17821 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
    17881 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
    17941 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
    18001 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
    18061 cagcgcctat gattgctact ttcttcattt atttctccct cctttgctta tttacttatt
    18121 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
    18181 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
    18241 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
    18301 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
    18361 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
    18421 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
    18481 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
    18541 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
    18601 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
    18661 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
    18721 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
    18781 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
    18841 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
    18901 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
    18961 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
    19021 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
    19081 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
    19141 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
    19201 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
    19261 taagaaatga tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg
    19321 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    19381 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct
    19441 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    19501 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    19561 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    19621 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    19681 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    19741 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    19801 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    19861 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    19921 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    19981 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    20041 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    20101 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    20161 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    20221 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    20281 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    20341 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    20401 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    20461 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    20521 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    20581 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    20641 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    20701 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    20761 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    20821 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    20881 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    20941 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    21001 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    21061 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    21121 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    21181 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    21241 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    21301 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    21361 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    21421 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    21481 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    21541 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    21601 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    21661 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    21721 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    21781 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    21841 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    21901 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    21961 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    22021 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    22081 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    22141 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    22201 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    22261 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    22321 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    22381 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    22441 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    22501 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    22561 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    22621 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    22681 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    22741 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    22801 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    22861 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    22921 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    22981 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    23041 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    23101 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    23161 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    23221 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    23281 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    23341 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    23401 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    23461 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    23521 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    23581 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    23641 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    23701 gtagtttact cgggtaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    23761 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    23821 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    23881 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    23941 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    24001 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    24061 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    24121 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    24181 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    24241 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    24301 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    24361 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    24421 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    24481 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    24541 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    24601 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    24661 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    24721 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    24781 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    24841 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    24901 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    24961 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    25021 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    25081 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    25141 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    25201 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    25261 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    25321 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    25381 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    25441 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    25501 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    25561 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    25621 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    25681 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    25741 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    25801 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    25861 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    25921 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    25981 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    26041 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    26101 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    26161 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    26221 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    26281 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    26341 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    26401 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    26461 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    26521 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    26581 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    26641 agac
//
LOCUS       DACCXW010000028        26389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCXW010000028 DACCXW010000000
VERSION     DACCXW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.028"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            123..419
                     /locus_tag="IVY71_000968"
     CDS             123..419
                     /locus_tag="IVY71_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7985035.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1063..2016)
                     /locus_tag="IVY71_000969"
     CDS             complement(1063..2016)
                     /locus_tag="IVY71_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7985036.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(2039..2794)
                     /locus_tag="IVY71_000970"
     CDS             complement(2039..2794)
                     /locus_tag="IVY71_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7985037.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2791..3750)
                     /locus_tag="IVY71_000971"
     CDS             complement(2791..3750)
                     /locus_tag="IVY71_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7985038.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3743..4693)
                     /locus_tag="IVY71_000972"
     CDS             complement(3743..4693)
                     /locus_tag="IVY71_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7985039.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4843..5295
                     /locus_tag="IVY71_000973"
     CDS             4843..5295
                     /locus_tag="IVY71_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7985040.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5384..7651)
                     /gene="gshAB"
                     /locus_tag="IVY71_000974"
     CDS             complement(5384..7651)
                     /gene="gshAB"
                     /locus_tag="IVY71_000974"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7985041.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7838..8836)
                     /gene="ftsY"
                     /locus_tag="IVY71_000975"
     CDS             complement(7838..8836)
                     /gene="ftsY"
                     /locus_tag="IVY71_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7985042.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8847..9656)
                     /locus_tag="IVY71_000976"
     CDS             complement(8847..9656)
                     /locus_tag="IVY71_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7985043.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9665..13246)
                     /gene="smc"
                     /locus_tag="IVY71_000977"
     CDS             complement(9665..13246)
                     /gene="smc"
                     /locus_tag="IVY71_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7985044.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13266..13952)
                     /locus_tag="IVY71_000978"
     CDS             complement(13266..13952)
                     /locus_tag="IVY71_000978"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7985045.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14300..16081)
                     /locus_tag="IVY71_000979"
     CDS             complement(14300..16081)
                     /locus_tag="IVY71_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7985046.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16107..17021)
                     /locus_tag="IVY71_000980"
     CDS             complement(16107..17021)
                     /locus_tag="IVY71_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7985047.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(17048..18010)
                     /locus_tag="IVY71_000981"
     CDS             complement(17048..18010)
                     /locus_tag="IVY71_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7985048.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(18010..18954)
                     /locus_tag="IVY71_000982"
     CDS             complement(18010..18954)
                     /locus_tag="IVY71_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7985049.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18955..19959)
                     /locus_tag="IVY71_000983"
     CDS             complement(18955..19959)
                     /locus_tag="IVY71_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7985050.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(20227..20469)
                     /gene="acpP"
                     /locus_tag="IVY71_000984"
     CDS             complement(20227..20469)
                     /gene="acpP"
                     /locus_tag="IVY71_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7985051.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(20591..21592)
                     /gene="plsX"
                     /locus_tag="IVY71_000985"
     CDS             complement(20591..21592)
                     /gene="plsX"
                     /locus_tag="IVY71_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7985052.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(21641..23677)
                     /gene="recG"
                     /locus_tag="IVY71_000986"
     CDS             complement(21641..23677)
                     /gene="recG"
                     /locus_tag="IVY71_000986"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7985053.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23794..25476)
                     /locus_tag="IVY71_000987"
     CDS             complement(23794..25476)
                     /locus_tag="IVY71_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7985054.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(25528..25890)
                     /locus_tag="IVY71_000988"
     CDS             complement(25528..25890)
                     /locus_tag="IVY71_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7985055.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            26160..26348
                     /locus_tag="IVY71_000989"
     CDS             26160..26348
                     /locus_tag="IVY71_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7985056.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7280 a         5730 c         4658 g         8721 t
ORIGIN      
        1 ctataaaaaa taaaaaacct ttaacaacta ttggaaaaag aattactttt tgttcacgga
       61 ttattaacaa ctcttcggta gagtaaactt atcaaataaa agacttcttt ggaggaatca
      121 ctatgaagga atatataaaa aacatctatt ttatcgaaga aacacaaaat atcgagggtt
      181 cttatattga agtaaaaaca ctctttgtca atgaagataa aaccaaagca ttggatattt
      241 ataaaaaatt agcttcaaaa aaaacgaata gttttggatt gatattaagc gagtataaaa
      301 tcaaagcaga agagagttat ttctatcaat tactaaaacg gtggagtaag cttcccgccg
      361 atttttatcg aaaaatgcag atcatcaact atcaaccgct tgctgagaca catgcctaaa
      421 tagaggttgt gaaatcagtg ctttgagcta ataccgaagg tcagctgatt gaacattgtt
      481 tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg acagaaaata
      541 gcttctcata ttttctagtc tagtgggctt attgccgaga agcagcagat ggaacatcgt
      601 ttagaagaga ttgtgacaac tgcgatttgt tttgagtaat aagaacgctg gacagaaaat
      661 agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga tggaacatcg
      721 tttaaagaag agatcggtac aaaataaaaa tatctaaatt taagttttat ctaagtttcg
      781 ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa tggatcacac
      841 tattttttgc cactccccat tctagaatca acccgtatga tgtagataat gaattcacgt
      901 tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa atcatcaata cgattttacg
      961 gatggttttt gataacttct ttgaggatag gctcttgcta tttaagaatg taagaaacat
     1021 tattgttaca tagtatgaga gtttaatcct ctgttattta tgctatttaa atgcttgatt
     1081 cacatcttcg atcatcagtt tcattgattc taaaccgccc ccgcttaaat accagacatc
     1141 tggttcaagg gagatgatct gctgattttt accagcattc gtttgtgcta ccagttcatt
     1201 ggcagaaata tcatctttgc tgtcgtctcc accaatagct tttgttctgt ctactacaaa
     1261 taagatatcc ggattttttt ccagcacata ttcataagaa acactttgac cgtgagtgga
     1321 tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa tcatggatga aaccgaatct
     1381 agaaccagat ccgtaggctg ataattgccc ttcattcaca agtgtgacta gtgctttttt
     1441 gtcagtagct tctgctttct ttttcgtctg atcgattgca ctagataatt cctctaattg
     1501 agttttagct tcttcttgct taccaaagat ctcccctaaa gcagtgacat tttgctggaa
     1561 agattcccat ggatttttcg catctaaaga aaggaaaatg gtcggagcaa tttgacttag
     1621 atctttttga aaatctcttt gtcggccaga aatgatgatt agatctggct gaagctgatt
     1681 aattttttct aagtctggtt cttttattcc acctgctgat tctacttttt tataggatga
     1741 aagatactca ggaaggctgc ttgtagctgc tccaactacc ttatctccaa caccaagagc
     1801 atccatcgta tctaaagaac cattgtcgaa tacgacgact ttttctggat tatttggcac
     1861 ctcgatttgg tctccattac tatctgttat cgtcaatgta gtttcagttt gtgtgctgct
     1921 tttttttgtg tctgcctgac tggtgttgtt gctatttcca catgctgcta aaccaaatga
     1981 tgctagaatc aagataccta ctactaattt tttcatctct tctctcctaa tgttagtttt
     2041 agaaataaag acagaatctg cggccttcaa tctcacaaat ccgtatgtcc atttcgtata
     2101 attcatccaa tacatctttt tttatcattt cttgggtcgt tccttgcgta aataactgtc
     2161 catctttcat tgcaacaatt tcgtctgcat aacttgcagc aaagttgata tcatgaagaa
     2221 caataacaat tgttttcccg atttcttgta ctaaattttt taaaatctgc atgatttgaa
     2281 ccgcaaaatt catatctaga ttgttcaaag gttcatccag taaaatataa tccgtgtctt
     2341 gcgctagcac cattgcaata tacgcacgtt ggagttgacc gcctgaaaga gtgtcaatcg
     2401 attgatccgc tatttcttct aacccaaggt gttccagtga ttcctgaacg atttcctcgt
     2461 cttttgtcgt caattttcct ttcgtataag gaaatcgtcc aaattctact aattctttca
     2521 cggtgatttg gacatttatt tcattcgatt gtcgtaggat cgataatttt ttggctaatt
     2581 cattggattt ccaagccttt acttcgttat ggtctaaata gatttcacct gtatcttttg
     2641 gtatcaaacg actgatcatc gacaacaccg ttgactttcc agcaccattt ggaccgataa
     2701 aagcagtcaa cttcttttca ggaatcgtta actgtacatc tttaaccact gattttccat
     2761 ggtacagttt attgaccttt tgtaaatcaa tcataggata ctcttccttt cgcgaatcaa
     2821 ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg acactcactg ttgtattcca
     2881 gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga aacagcatag ctaccagaaa
     2941 gcttccgata aatagctgac catgacgata agtagaaaag acacgataac tgatatttgc
     3001 gacaataaac cctaaaaaag taactggacc cacaagagcc gttgatgtcc ctgtcaaaag
     3061 actgacacaa cataaaatca aaaattcaaa acgatctgtg tctatcccga ggtttactgc
     3121 ttgttcattt ccgagatgga aaacgtctag cacatgtcga tatcgaaata acaggtacca
     3181 gcagccgata atgatcaaag aagcaggcaa aagatactga ctctggatat tgccaaagct
     3241 tgcaaataat ctgccttgaa gcaaatcata ttcattcgga tccatgatga cttgtaaaaa
     3301 ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct gacatcagta ataaaaaaag
     3361 attcgctctc atttttttca aaagaaagaa aaataacaat agactcagcc cgaccatcag
     3421 cccaacgttc aataagaaaa aaactgtacc tgtctgctga agcatcgtca ctcctccgat
     3481 aaagaagaaa aggaccgttt ggacaaggac atataacgac tcaagtccca atactgaagg
     3541 agtcaaaaac tggttatgcg taattgtctg aaagctgatt gtggcactgc tgacagctag
     3601 cgagacaagg ataaatgcaa aaattttctt actcctcaat gcacatgcaa aatgccagtt
     3661 tccatatgta tggatcgtca agtacattcc tgtaacaaat aaaacaagaa caagcatacc
     3721 aataaaaata gatggctttt ttttaagcat atcgtcgtcc ccttatcaga agataaagga
     3781 agatagcccc accgatgatt ccaacgatca cactgactgg tatttcataa ggaaaaatca
     3841 acacgcgagc gacaagatcg caaaatacta agaacaaact acctgctaaa gcagttacgc
     3901 caattgtttt gtgcacttga tctccatata ttctagagac aagattcgga atgatcactc
     3961 cgataaaagg aatgcttcct accatgatca acatgactgc actagccaat gatactaagc
     4021 caatgcctaa aaactgcatc ttctggtaat ctattcccag atttactgca aacgattcgc
     4081 ccagaccaat gatcgtaaat cggtaagcaa atagccaaac caagagcaac aatggaacaa
     4141 cagcatacaa taactcataa tttcctcgta tcacagtcgc aaaattccct tgaagccatg
     4201 aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa agtagcaact gaaccaatga
     4261 tattccctaa catgactcca atcaacggaa gcatcaattg atttttctgg aggaacaatc
     4321 gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct gtcaaggatc
     4381 ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac taaaatacct aaacgcgcac
     4441 tatccatcgt gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa tgttgcatga
     4501 tcaacccaca aatactactg gtcgctccca caaggatcaa acaaatcgtt cgtggcaaac
     4561 gagtcactag caagatttgt aagctttgcc catctaactg gagcagttct gacaattgta
     4621 cattcgatac tccgatgaac aatgaaatac tacttaaaat aactaataat gctactagtc
     4681 gtccgtagcg catccaatcc acctttctaa ttgagaatga ttttcattct tattttaagt
     4741 gaatcgttga acaaaagcaa ccgctttttc atatgtttta ggtattattg ggaatatgct
     4801 ataatcgtaa gaaagacaaa gagaagaagg agggtatttt ttatggcaca agatgatgta
     4861 aaaagccatt tggataaggg actttatggt acacctttag tgaaccctga agaacagcat
     4921 aaatatttag ggacattcag ggaacgctgc tatcttagta tgactgttgc tgaaatgatc
     4981 caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga tgcttccata
     5041 ctcttgaatg gggctctacc cctttctgtc caaaaccaat atatccagct agcaacgaaa
     5101 aacaatagcc gtttcactgt agtcaacgat tacgtcactg caaatcctga ggaattcgga
     5161 ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga ttgatatcga aaaaaaatac
     5221 ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa aagcttctgg
     5281 gatcgtttat tttaaaacga aaaaacacat aaatttttac atgcaaaaaa agcttttgat
     5341 cagattgaca tcaaatagga caatctgtca aaagcttttt cttttaaaat acttcaggat
     5401 ataacaattt caatacattc atcgtcaatc ttctgccttt tcctgcaaaa ggataaacat
     5461 gcatatgcat ggctggattg aaatttgctt cgatgatccc atatgctttt ttatcagttg
     5521 taggatcgat ttctttatct ggtataatca aatcgatacc actaatctta gctcctagtg
     5581 cttctaccgc agaaacggcg atttttttgt aagcttcaga aaattcatca gtcatatcga
     5641 tcgaatcacc acctgtactg atatttgagt tctcccttag ataaacaatt tgattggctt
     5701 ggggaactga ttcgatcgtc aacccttgtt ctttcaacat caattgttct aattcaccta
     5761 attggatcaa ttctaaaggt gcacgatgat tcgttccacg caaagggtca ctattttttt
     5821 cttcaacaag ttctttcact gttcggatac tgtcaccaat aacattagca gggactcgca
     5881 acatgatggc ttggacttct ccgtcgatca caaagaaacg atattccgtt cccggcataa
     5941 attcttctac taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct gcttgataat
     6001 cttccagact agctccttcc ttgaaaatcg tgatacctaa cccgtaattg gttgatttag
     6061 gtttgatcac aaatgcctgc tcggcaaatc tcggataagc ttttaccgct tcttccatgg
     6121 atgaaaattc tgcgcctcct ggtacccgga acccagcttc ttttaagact tttttcgtta
     6181 ctgttttgtt ttccatgata agtgggacga tatagctgtc cttacttgtc atgtttgcat
     6241 ttttcacata ttctacatgg tcttggaatt gcagacgcaa aaattgatcc gactcatcta
     6301 atactttgac ttggacacct ttttggatgg catcgaacat aaatatctga gtagaaagtt
     6361 ccatttcgcg aaaaccggcc aactgatagg gacgttcatg gctttcttta taatattgat
     6421 gtcccaatgc cacagcaact tcttgctgac tcgatagctg tgcttcttga tagagtttcg
     6481 ctgctaacgt ttgttcagga tgatccattt gagtcaatgc ttcctctact aaataaagag
     6541 aatccaacca atccaattca accagcattt gcttcattcc ttcaagaatt tctctacctt
     6601 cttgatgaaa ttctgtttga tctgatggtt gttctagtgc tactttgtta tttcgagtcg
     6661 taccttctgc tacccattgg tcacagtctt ctttttcttc tgtccataac atgtacatca
     6721 ggaataattg taagaaccga acttgctctg gattgatacc caatcttgca taaggattca
     6781 aatcgatatt ccgcaattca atataacgaa cacctgttgt tgctaaatct gcgactttct
     6841 ttccgcctcg gaaacgaagc ggtgtataaa attctttctc ctccgaaagt ttcccttctt
     6901 cgatcattcg ctcgatatct gctaaatact gtttcataga agcataggaa actttcacat
     6961 ttggatgatt cgtatagccc agcgcactgt tgcgaatact gcgaacaggt tcttgcggat
     7021 gtgattcatc taaaaagtag ttcttctcac tcattggtga agcaccgaat aaataagtga
     7081 tcatccagcg atagcgcata aagtttctcg ctgttttcaa ataaagttct gttttgaatt
     7141 cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt tcttagcaac tcatcgccaa
     7201 attcaaaatt gaaatgaata ccgcttacca tctgttttcg cttcccgtat ttttttgcta
     7261 aataacgtcg atacaagaca tcttcgaaat tttttaattt tgcaataatg atctcttcgt
     7321 ctttttccgg taaggcaggt ggcatactta atggccagat catctcttct ttcggtatag
     7381 aacgagcagt cacatcataa acagcagcga gatactgaaa taattcggga atagaatcgg
     7441 taacaggggt gatcatctct gtttgtgttt cgctaaaatc tgtttggata taggggtgat
     7501 aggatcgatc gccaaagatt gctggatgat cggtttttgc tagatttcct gcaagatcaa
     7561 cccgctgtcc ttcccgttct ataccaaatc gagcttggtc gataaaaggg cgcgcattga
     7621 catgcaataa taattgctta aaattcatca ttaagtgacc tccaattcat atgaatctaa
     7681 ttataatcaa aaagtctaag aaaaagaacg aaattgtttg agaaccgctt acaaataagt
     7741 ataaaaatga aggcatcaaa aacacagcta tgttaaaaac aatcattcaa acaaggagct
     7801 gcaacttttg tcacagctcc tgctttttag acatgggtta ttcttctttt agtaatcctt
     7861 tgaacaatcc aacgacaaaa tcatttggat caaacggctc caagtcatcg attccttcac
     7921 caagaccgac caatttcact ggtaaatgca attcattacg gatagcaagt acgataccgc
     7981 cctttgctgt accatctaat tttgtcaaaa caagaccggt cacatctgtt gtttctttga
     8041 attgttttgc ttgaaccatc gcgttttgac cagtggttgc atctaataca agaagtgttt
     8101 cttggggtgc ttctggtaat tcccgctgga ttactcgttt tattttttct aattcgttca
     8161 tcaagttcac tttattttgc agtcggcctg cagtatcaac tagtaaaaca tctgcctgct
     8221 ctgtctttgc tctttccaaa gcatcgaata cgacagctgc tggatcgcct cctgcattgc
     8281 cacgaacaac ttcaacacca gcgcgttcgc cccagacaac taactggtcg attgctccag
     8341 cgcggaaggt atctgctgct gccatcaata cttttttacc ttcgtttttg aactgatggg
     8401 caagtttacc aatacttgtt gttttcccta caccattaac accgacaaac aaaataactg
     8461 ttagtccgtc ttcttggata tttagttcat ttacttcatt gattccttcc gcttcataga
     8521 gatccacaag tttctcgatg atcgcatttt ggacttctgc cgattttttc gcatttctga
     8581 gtttcacttc ttgacgtaat gcttcggtga ttcgtatcgc tgtttcaaaa cctacatctg
     8641 caccgataag tgtttcttct aattcttcaa aaaagtcttc atcgactgtt cgaaaattgg
     8701 caaacaactc attcatccgt tcaccaaatg ttttacgcgt cttttctaaa cccttgtcgt
     8761 acttctcctg tacttcaact tcttctgttt tctcttcgcc agtaaaagca cgcttgattt
     8821 tatcaaaaaa tcccatttga ctccccctac tctaatacaa atttttggat cactttggct
     8881 acgccatttt cttcattgct ggctgtcacg aaatctgctg cttctttgac gaaaggcaca
     8941 gcattctcca tcgctacgcc tagtccagca tattggatca tcggtaaatc attttcctca
     9001 tcaccgatcg ccatgatttc ttcttgtttc aaaccaagat cttttgccaa taaagaaata
     9061 ccataagctt ttgtaacacc ttttggcata aattccaaaa gcatacttct tgttttgatg
     9121 atctcataca tttcatggaa ttctgctggt atttctttga ttttaggatc taattcttct
     9181 tggtcataag cgattactgc cttattaagc acaaattcat ctgtaatatc gcttatatcc
     9241 gctggttgga attgcagcag cttattcaac acattgtaca aggattgctt ccttggggca
     9301 ctaggcagct gtaaaaccac attgtcagac aaaacatcta gtggcagatc caattgctga
     9361 gccagtttgt ataagcgttg gatatctgat acttccatca ctttcttttc catgatttca
     9421 ccagtatcat ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc accttcatct
     9481 accagtccta attcttgcag ataatgcgcc atcgcagcta aggggcgtcc tgtacataga
     9541 acgatcttga ccccttgctg ttttgcttgt gctaatgctt gtttattctc atctgaaatc
     9601 agcttttgtt catttaataa tgtaccgtcc aaatcgattg ctactaattt gatcattttc
     9661 gttcctacac tttcgtttcg ttgattttcg cctcttcatt gatatcttcc atccgtacag
     9721 aaacgatttt tgatacaccg gattcctgca tcgtcactcc gtacagtaca tctgctgcga
     9781 ccatcgttcc tttgcggtgt gtgactacga taaattgtgt gtcatcttgg aaatcgctca
     9841 agtaacgacc aaaacgtttc acgttggctt catctaacgc tgcttctacc tcatctaaga
     9901 tacaaaacgg aacaggacag acacggataa tggaaaagag caacgcgatg gctgtaagtg
     9961 ctcgttctcc tccagaaagc aaacttagac tttgcagttt tttgccagga ggttgtactt
    10021 caatctcaat acctgttttc agcaaatcag ttggttccgt cagtactaat tcagctttac
    10081 cgccgccaaa catatttgga aagacaattt tgaattcttg acggatagct tcaaatactt
    10141 ctttgaatct tgtccgcact tcatcatcca tctcatccat cgtttcaaac aattggtttt
    10201 tcgctgctaa cagatcatct cgctgtgttg ctaagaatgt atgtctttca ctgacttgtt
    10261 cgtattgttc gattgaattt aagtttactg gtcccagttt ttcaatctgt tcttttaagg
    10321 atgaaacctt tgttctactg tcttcgatat cggttgtttc ttgatagtct tgggaagctt
    10381 tctcaaatgt cagctgatat tctgactgca gataggtcaa atgattatct aaaatcattt
    10441 cagctcggtc tttttggact tctaatttgg attgttccga cagtcgtgct ttttgctcac
    10501 gattacgctc tgccaagact gcttcaagct tatcgatctc cttttgcctt ctatctcgct
    10561 gctctttggc ttttgccaac tcggctttta atgtttctcg ctgagcagat aattcgttaa
    10621 tttgtttttc caaactttct tctgtgactt catgatcaga aaagtctgct gtcaatgtcg
    10681 ccaactgctt ttctatcgct tcttgacgtt cggttgcctc tgctttttgg actcgggcac
    10741 cgcggagctg gatctgcaga tgattaaact gctctttcaa gacagcaaga tctgcttgtt
    10801 cttgtgcttt ttgactttgt acttgtgctc ttctggcttc catctggtcg ctttcttgac
    10861 ttaacgattt gatttcttcg tcgatttttt gtcgttgcga ttcaagatct gtttgtttgt
    10921 ctttcaattc gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt tcacgtgtct
    10981 caaaagttga gatttgcttt tctttttcaa aacgttcaag ttcatttatg atgttttgta
    11041 gctgatttgt cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta cgcaaaactt
    11101 cttgttcttc ggctaaacga gctgtttctt gttgcagctc ctgaactttt ttttcttgtg
    11161 cttggagttg tttatctgct tcttcaaatt cactagtcaa ctgtttcaat tcttgatttt
    11221 gtacaaacaa actgccttgg tttcctcgtt tattcgctcc acctgtcatg gatcctccgg
    11281 cattcatcac atcaccttct aaggaaacca ctcgatattg atagcggatc gtttgcgcga
    11341 ttgcattggc actagttaag tctttggcta aaaggatcgt tcctagcagg ttttgaacca
    11401 cagtttggat ttgatcggga taactgactt gttcactggc aatccctata aacccttcta
    11461 cagctgctgc ttgtgtcaat atatgagccg gtaattgtct tggtttgatt gtcgtcagtg
    11521 gaaggaatgt tgctcttcct cctctttgct gtttcaaata cgtgatggct tgccgagcat
    11581 ctttttcatt ttcaacgatg acatgctggg ctgccccacc taaagctgct tcgatagcta
    11641 acgtgaaatc tgcaggaaca tcgatcagtt cagccactgc tccgacaata cccgaaagct
    11701 gctgtttatg ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag ttttcttgga
    11761 tttcttgaag gcttttctta cgagcacgta gctgctggac ttgattcatt aattgataca
    11821 ttttaggctg ttcttttgct aatgcttctt ggaacttttt cccgttttgt tggatcattt
    11881 ctaacttctc ttttgtttca gttaaagatg attttagctg cgcctgcttt tgtgtcaatt
    11941 cttctttttg taagatcaat cggtctactg atgcttctac ttccgattgc ttggctaacg
    12001 tctgcttgct ttttgctgtt tcttgtatat attgacgttc tagatacttc agttcgtttc
    12061 ctacagctgc ttgttcttgc atcaaatcaa catattgatc tcttaattct gccaataatt
    12121 ctttcgttga tttttggtat ttttctaatt cttgctgtgt ttttatgaga tttgcttcag
    12181 ctttttggat ctctgtttct ttttctgctg ctgctttcat caagctttct tgaagctttt
    12241 caaaatgttt aacctttttt tgtgcttctg ctaatgaagt ttgatattct tgcgagcttt
    12301 tttgtgtatg ctttgtccgc tcctgcaaaa catctttttg tccttctgtc tgcttcagtt
    12361 tttccgaaag atctaataat acttgctgat ttttttcgat cagacgatcg gcttgcgcat
    12421 tttctttacg ctgcttcgcc aaaatggatt cttgttcttg gatcgactca gatagtttgc
    12481 ctaattctag attgaatttc gcaagctgtg cttgtttgtt gtcccaatct ttttttgctg
    12541 tcttgatttc agcaaccatc aaagagacat cggtttgagt cagtgtttct tttagccgta
    12601 aaaactcctt tgctgcctca ctctgggcgg ccaaaggagt taactgttct tccaattcgt
    12661 ggatgatgtc ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc tgttctgctt
    12721 tctttttccg ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc ccacgacggt
    12781 cttccggttt actactgaaa atcgcttcta cttttccttg agaaatgatt gagaacgatt
    12841 cttttcctaa tccagaatct aagaacaatt cttggatatc ttttaatctg cagctttgtt
    12901 tattgatgaa aaactcgctt tccccagtcc gtcgataacg gcgggttaca ctgatctcat
    12961 tgtactccaa tggcagataa tgatcactat tgtctaaaat cactgtgact tctgcaatat
    13021 tcagctgctt acgggtatcg gaaccggcaa agatgatatc cggcattttt ccgcctcgca
    13081 gacttttagc agattgttct cctaataccc agcggatagc ctctgtaata ttgctttttc
    13141 cgctgccatt tggtccaacc actgctgtga cgctattttc aaaatcaatg actgtcttgt
    13201 ctgcaaatga tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct ttccctcact
    13261 tacttttaca gtgctgccaa tgcattttcc gccgcagctt gttctgctaa ttttttcgat
    13321 ttcccttgtc cagtgccaat caattgatca tctacataaa cttcgatcca aaatactcgt
    13381 tcatgagctg gaccttcttc attgatcaat cggtattcga ttgatacatc acctgatttt
    13441 tgcaagactt cttgtagttt tgttttatga tccatctcat gtgaaaaagc acctgctttg
    13501 actttaggaa aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc ctggtcaaga
    13561 tataacgcgc ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg cgtacgtcct
    13621 cctgaattct cttctccctt gcccaacaaa acatattgat caaagtgaca ttctttggca
    13681 aatttcgaca agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa ttttccttct
    13741 ggcaaatgag gatataaacg gaataaatac tgagaaacca tcagttctaa aacggcatct
    13801 cctaaaaatt caagtcgctc attatccgat aattgcagat tgcgatgctc attcacatag
    13861 gatgaatggg taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat gttatagttt
    13921 tcttttagtt cttttgtcag ctggttgtcc attttccaca ttccttttat ggatattttt
    13981 tcgatacttc cttattatac tggttttttg tcaaatttga aatggttcgg tcaaaaggta
    14041 agtaaagctt taataatcct agtgattgac gagaagttgt gaaaaattcg tttagtttcg
    14101 agtatgaaga aaaaattttg tgaaaatggc tttccatatt tttgcataaa tttgacttaa
    14161 taccgagaaa ctggactttg aacaccgttt attggagaag tcatgaaatc agcgccttga
    14221 cctgaatatt agagaaataa acagaaaaat atcaaaaaac cgaaaaagga catcgccctt
    14281 tttcggtcat ttttagtgat tatgaacgtg aatctgctgt aacagaaaca tttgcccatg
    14341 ggtttggtac atcataagac caatcccagt ctttgacacg gtcgttgact ggcaatactt
    14401 cattacggaa caacgttgga atcgcaaatg cttcttcaaa tgcatattgt tgccattttt
    14461 gatatgcttc tttttggtac tcttcatcaa atgctttgct tgaactgata tcgttgatca
    14521 attttgtatt ttcttctgat tcataacgag tgtagttgaa cgcagcattt ggtccccata
    14581 gtccacttgg atttggatca gttcctgtat tccaagcagc ttggtaaaca tcaatttcag
    14641 gatcatcgtt ttctaattta tcatagaaag aattgaattc gatcagacga cctgttgtgt
    14701 attggacatc caaaccaatt tctttccatt gttggatata gtaatctgcc aatggttgcg
    14761 ctgtttcccc accggacatc gaagcaaagt tgattgttag tttatttcca tctttgtctt
    14821 ctcttaaacc atcgtcatcc acatctttgt agccagcatc atctaatagt tttttcgctt
    14881 tgtctagatc ttgtgtgaat cctttcatct catcatcatg gtatgactgg aatactggag
    14941 gaatcaatga agtcgcattg cttctcaaac ctgcatagaa acgttcacct actgcattat
    15001 tatctaatgc atagcccatt gcttgacgta atgatttatc agccattttt gaatctggat
    15061 tgtattccac actctttgta tctgcattcc atttacctaa cttgaatcct agataagtat
    15121 aagaagtttg ttcacggcct aagtttgtat agccagcaat ttctttatag gtatcgtaag
    15181 tatctgttgg catttggaaa atcatatcat ataatttaga gttcaatgct tcagttgcac
    15241 ttgccatcgg tacagatttc atcgtgatct ttttcaattt aggcgtacca ttccagtagt
    15301 gctcgtttgg caagtattct actgattctc ctgcaacgat attactcatg taataagggc
    15361 caaaagtcac tggattttta cgtacttgat cgcttgattc catatctttg attgctacac
    15421 cttctaacgt atgttttggt gctgcatatg accagatacc accacccgat tgaagcatgc
    15481 ttgggtttac ttctttatat tcgatttcaa ctgttttgtc gtctattttt ttgattccgg
    15541 agattgtgtc ggcacttcca tcatgatatt cttccatacc tacgatgtta cggaaagtgt
    15601 cgtcataacg gataccagtg taatctttat tcccaatgat ttcgtaaggg aaaatcacat
    15661 cgtccgccgt tacatcttca ccatctgacc atttgacgtt gtcttttact ttgattgtcg
    15721 cttttttcgt atcttcatct agatcaagcg ttgcagctcc gtcgtctgtg attctgaaat
    15781 cgtcatcgtt tgcaaatagc ggttcatgag aaggagccat aaaagctgca tcatatgaat
    15841 cttggtaaaa ctcccactgg aacaatcctt taaactgact gtccgttgcg accgcagctt
    15901 ctaattcgcc gtcttcgatt gcatcatcat tattttttgt tgcaattgga aaagttaatt
    15961 cctctttgtt attgccgttg cctccagagt tgtttccaga ggtatctgta cctccgcccc
    16021 cgcatgctgc aagaacgaca gcagacatca gcgctgcaaa tccaaaaatc gttttcttca
    16081 ttcctatttc ccccttcgct tctatactag cctaaccgtt ggcgcgcatc tgcagaacgc
    16141 ttgaatgctt gtccaacata attgatactc aacatcaaaa ctaaaataag gattgatgcg
    16201 ggtaaccaga tccattgccg attcactaag acttctccgt tacttgcata accgatcaat
    16261 gtacctaaac taggtacatt tgccggcaag ccgaaaccta agaaagtcaa agttgtctcg
    16321 ataccgatgt ttgccgcaaa gttcatcgtc aaattagtaa tgattaatga acttagattc
    16381 ggcatgattt cgcggaacat gattttaaaa tcattggttc ccattgtttt tgaggcactg
    16441 acataatctc ttcttgcttc agataatgtc ttgcttcgga ataatcgggc tttagctacc
    16501 caataaaatg cactcatgat gaatataaag gaccagacat tgtatctgga aataatcgat
    16561 acgaagacga taatgatcag catgatcggt agaatcatga taaaatcaac gatccgcatc
    16621 aagatgttgt caatgatccc tccgtagtag ccagagatga tccctaatcc aaccccgatg
    16681 atcgaagtaa ggatcgtgat cgcaaatccg atcgtaacag agtttctagc acctatgatc
    16741 aattgtccta atacatcacg tccgccttca tctgctccca gaatgaactt ttgtccttgt
    16801 gtcgaaactg ttcctggtgc agcgtatttg tcgaagatac taacatacat cactttgtct
    16861 tgatcggtca gcatcgcacc gataaagatc actaataata aaaccactag caaacctaat
    16921 gaaaaaatag caattttatc ttttttaaat tctcgagcaa tcattcgtat ccccatagga
    16981 ggaatacttt cttgaactgc tgtattgttt ttttcactca tcgatccatt ctcctttctg
    17041 attggtttta ttgaatccga atacgcgggt caacgatact catgatgata tctgaaagta
    17101 atgtccccac tagcgtcgcg attcctaaaa tcaatactaa ggatgtgatc acggtatagt
    17161 cccgttggcc gatggaatca ataaatagtt ttccgactcc cggataacca aaaatctttt
    17221 cgataactac agaaccagca atcaacgaag tgatttcata ccccatttga gaagccatcg
    17281 gcaacgctgc atttcggaaa atatgtcgac tgaatacttt attcgtcggt acccctttgg
    17341 aacgggctgt tctgacaaaa tcgagcgatt tcgaatcgat tacttcactt cgcagatatt
    17401 ggattgtgac ggcagtcgcc aaaaatgctt gggtaattga tggtaacaga atatatttca
    17461 aacggtctat ccattgagtg ataaatccac cttgcaaacc tagtgtttgt gatccacttg
    17521 ttgggaacca gcctaaacga taaccaaaaa caaatagcat gatcaagccg aagataaata
    17581 aaggaacagc aaaactaaag aagttgtaga ttacgacaat cttatccaag attgaatttt
    17641 gataccggcc agcaagcatc cccagaggca cagcaatcaa ataagtaagg acaactgtca
    17701 agatcgatag atataaagtg ttgatgatcc gttcgccgat caagtcagca acagagcgtt
    17761 tgtataagaa actttgacca aaatcgcctt gcaaagcatt tccgatccaa cggaagtact
    17821 ggacatacca tgggtcattc aagccagctg cttgtcgcat ctgttcgatc actgccggat
    17881 cctgatttgg attgatcaaa ccggtaaatg gatcccccgg catcgcttgg gctaataaga
    17941 agatcagaac acttaaaata ataacttgcg ggatcatcaa taaaacacgt cgcagaatcg
    18001 ttttccacat tagcttgcac ctccatcttt caacgccact ttgtgtgtag ttgtgatcgt
    18061 acgcaaatcg tagactcgac cagtcgcatc atagtattct ttttggtttt ggatatactc
    18121 ttgttctact cttcgtcgat tttctttgtg gctttcccta ttttctacat cgatttgcgg
    18181 aatagctgaa agcagacgtt tcgtatagat atgtcgcggg tcattataaa tgtcttctct
    18241 tgtaccgatt tctacaaatc gacctttgta cataattgcg atattgtcgc acatgtgttt
    18301 tactacacct aaatcatgtg aaatgaataa atagctgagg ccgtattctt gctggattcg
    18361 tttcatgaaa ttcaatactt gcgcttgaac agatagatcc agagcagata caggttcatc
    18421 ggctacgatc agttttggat tggtagcaac agcacgggct actcctagac gttgacgttg
    18481 tccgcctgaa aattcatgag gatatttata tagcgcatct tccggcatcc caacgatatc
    18541 tagcagtcct tttacctttt tcttttcttc ttgatcgctt aaccgttcaa aatttcggat
    18601 gggttcagca atgatatcca acactcgctt tttaggattc aaactggaca ttgaatcttg
    18661 gaagatcatc tgcacatcct tgttataacc catttttttg cggttgcttc gcttcgttac
    18721 atcttttcct tcatacatga tttgtccggc tgttaccttt tctaatccca caacggcttt
    18781 tcccgtagta gattttccag agccagactc tcccactaaa ccatatgtct ttcctttttc
    18841 gataataaaa tccacaccat ctacagcata tacatgatcc gttactcgat tgaagaatcc
    18901 actgcgaata gggtaatgaa ctttcaggtc tttgattgtg attaattctg tcatctacac
    18961 ctctcctttt tcgtctctaa agtggaaatg cttatagcag gagcaacgta ccaagtggtt
    19021 cggtgcgact tcatgaagga taggctcttc ttcatgatcg cttgctggta tccatggaat
    19081 acgtggtgca aaccgacaac ctttacgagg catgtttttc agagaaggaa cgacaccttc
    19141 gatcacatgg agttctgcat catgatttcc ttcttgtggt atagagttca gtaaagagcg
    19201 tgtataagga tgcttaggat gatcaaacag ttcttttaca cctgctactt caacgaactg
    19261 tccgccatac atcactgcca ctcgatcagc catttctgct acgactccta agtcatgagt
    19321 gatcaagatg atccctgttt ttgtttcttc ctgaaggtct ttcagtaaat caagaatctg
    19381 tgcttggatg gttacatcta gggctgtcgt cggttcatct gcaatgacga ttggaggttt
    19441 gcaggcgatt gcgattgcga tgatgacacg ttgtctcata ccgcctgata gttcatgggg
    19501 atattggcgt ccaactctcg caggattagg aatccctact tgttctagca attctagtgc
    19561 acgagcttga cgctgttctg cattcatctt tgtgtgatag gaaagtcctt ctttgatctg
    19621 gtcttcgatt ctcatgagag gatttaatgc agacaacgga tcttggaaaa tcattccgat
    19681 atcatttccg cggattttat tatagagcgt ttcgttcagg tcgattaaat tcagatcgtt
    19741 atataaaatc tcacctgtaa tttttgtgtt attaggatca agtaatccca taatcgtagt
    19801 agccaaggta cttttcccgc acccagattc cccaacgatt gctaaaatct cgtttttgtc
    19861 taaagtaaat gaaacgtcat ctactgcatc gtaatagtca tctttgagtc ggaatcctgt
    19921 atgtaggttc tgaacttcta ataattggtt atcggacaat cgaatacccc tctctgcttc
    19981 ctaaatggaa gtttttctta ataaaaaagg acattacaca gaataaagcg ttttataaca
    20041 aaggttattc ttatctttgt gtaatgaatt attaataatt atatagaaaa tcgctttact
    20101 ttgcaacata atttctgttt atcgccagaa ttatcagaaa aatgactttt acgtgtcaaa
    20161 aagattaaaa aaagaaggat aaaaagctgt atgcagcttt ttatccttct ttgcttttat
    20221 ttgaaattat ttaagatggc tgctgatata atctactgcg ccaccgactg tttcaatttg
    20281 ttcagcgtct tcatctgaaa tttccgtacc gaattcatct tcaagctcta aaacgaattc
    20341 cataatacta attgaatcag cttttaaatc atctttgatg ttcaattcat ccgtcacttt
    20401 atcggtatct atctcaaaat gatttgaaat aattttcgct actttgttaa atacttcttc
    20461 gcgagtcaac tttttcacct ccaagaatca tacacttgtc aattacctac accatggcta
    20521 attttactgg catttttcag gaatgtccac ctttttttca atggaacgga ttgcttcaga
    20581 tatccaaata ctattcttcg cttttttcaa attgttcgac taattgtcca actacatctg
    20641 tctcaagcat tgtgtggatt tggcggatcg tatagcgaac agcatcgggg cctgttgctc
    20701 catgagtttt aatgactggt gctttcaagc caaacaaaac ggcaccgcca tgtttggaat
    20761 aatccatttc tgctttcaaa gaacgtaagc cgtcttttaa aagcatagcg cccatttttc
    20821 ctttgatccc ttcatttaga atagcagatt ttaatagatt catcatattc attgctgttc
    20881 cttcaataga tttcaaaaca gcgttgcctg taaatccatc tgtgaccact acatcagcca
    20941 caccattgag tagctcacga gcttctacgt ttccaataaa attcaagctt gtttcattct
    21001 gaaggagttc ataggctttt ttcgtcactt cactaccttt tgttgcttct gttccgttgt
    21061 ttaagagtgc gacacgagga cgtccgatac ctcggacttt acgtgcataa aaagaaccta
    21121 aaataccgta ttgcaacaaa tgttctggtt tgttttctgc gttagcgcca agatccagca
    21181 tatcgaaacc ggcaccttct ttccctacta ctggcaaggt agacatcaag ccagggcgtt
    21241 cgatattttt gatacgtcct acgatgaaca accctgctgc tagtagcgcg cctgtattcc
    21301 ctgctgaaaa tacagcatct gcttcaccgt ttttcactgc ttgtgcagca aggaccattg
    21361 atgcagtctt ttttcgtcga attgctttta ctggctcatc atcactattg attttttcgt
    21421 ctgtatggat aatcgtgatg tttttttcat ttgttaagta tttcttgatt tctgcttctt
    21481 ttccgtaaag taagaattct acttctggaa aatcttgctg cgctaaagcc acgccttcta
    21541 caattgcttg aggtgcgtga tctccgccca ttgcatctac ggcaattttc attgtttcca
    21601 ctcctttttc cgtcatcagt ataaagtata gcacagtctg ctaatcgaaa taacgattat
    21661 cctgattttc tatttggagg ctttgcgcta aaggctgata ctcaggaagc atctgccaat
    21721 cctttatctg ccaaagggct gcagcctctt ctctggctac ctccaaaaca tttccatccg
    21781 tcaccaagtc agcaatcgca aattgaggca gaccagattg acggaagcca aatacttcac
    21841 caggaccacg taattccaga tctttttcgc ttagtacaaa accattggtg gtctctgtca
    21901 tgattttcat ccgttccaca cctagttcat ttttaggatt agcaactaag atacagtagg
    21961 aagcatcact tccgcgtcca acacgtccac gaagttgatg cagctgtgcc aatccaaatc
    22021 gatccgcatc catgatcagc atgacggtcg cgttcggcac gtttacccct acttcaatta
    22081 cagtagtcga aactaatatc tgcatctggt tgtctttgaa ggcttccatg attacttctt
    22141 tttcctgatt cttcatttta ccatgtaaca agccaacttc atattttggt gcaaatagtt
    22201 cacgaagttt ttcatagatt tccactgcat tttttacatc taatgcttct gattcttcaa
    22261 tcagcggaca gatgacatac atttgatgtc ctcttgccag ttcttttttc gtccaatcca
    22321 gtacactgtc caattgaggt gttcttaccc agcgtgtttc gatcgggata cgtccagctg
    22381 gaagttcatc aatgatagat acatccatct caccatatgc tgtaatagct aatgttctag
    22441 gaatcggagt cgctgtcata aataagacat ctggtcgcca gcctttttca cgaagtactt
    22501 tccgctggtt cacgccaaaa cggtgctgct catccgtaat aactagacct aaattctgaa
    22561 attcgactcc atcttggatc aatgcatgtg tgccgatcac gatgttaatt tccccttgct
    22621 ctagtttttc aaggagctca cgacgttctt ttgctttcgt tgaccccgta agcaatgcga
    22681 tggttacttc attgggatca aacagctgtt gcaagctttc catatgttgt tgtgccaaga
    22741 tttctgtagg caccatcaac gcaccttgga aacctgctgt aacggtagcg tataacgcaa
    22801 tcgcggcaac cactgttttt ccgctaccta catctccctg caaaagtcgc tgcatatgct
    22861 gcggactgcg aagatcccga cagatttcat tcgttacacg tttttgtgca tttgtcagtt
    22921 caaaaggtag cttttgagta aattctttca atttttggac atcataaagg atctccaaac
    22981 cattcgtttc tgctttttct gcttttttca atccttgaag acgcatttga aataaaaaga
    23041 actcttcaaa aacgacacgt cgtttggctt gatggttttc ttcgtggtct tttggaaaat
    23101 gcatacttct tacagcagaa gctcggtcaa gcagtcgata tttctccaca aggttgtttg
    23161 gcagattttc ttcaatcaat gtgccgtatt cttgaaaccc ttttttgatt agatcaacta
    23221 atgtcgtttg acgaattgat ttgttgacgt gatatatcgg cgaaaagtca tccattgttt
    23281 gagaacctaa aatcttcatc ccgttcaatg ctttacgctt tgcatcccat ttaccgtata
    23341 cggcgatttc ctccgataaa atgacttttt cttttagata aggttgatta aaaaaagaaa
    23401 cgttgaacac atcatgttct tgcatcatac gaaattgcaa tcggcttttt ttataaccaa
    23461 atcgattcat cacaggaggc gagactacga ttcctttcaa agtaaccttt tcttgatctt
    23521 ggatctcgtt tagatttctt tcctgaatat cttcgtagcg gaaaggataa taagtcagta
    23581 agtcttcaat cgtttggatg cccaagctgg caagactagc tgctcttttt tctccgactc
    23641 ctgccagcat agtgattgga tcatctaaag aacgcataaa tttttcctcc ctcccttaac
    23701 aaaaaaagga cttggtcagc gatcatacct gatcactaac cgaaggcctt ttatgaatga
    23761 atactgtgtt agaaagatat tctgacacag aaattattca gcagaaaata gataaggata
    23821 gacaggctga tctccttggt ggatctcgat ttccaaatca ggatgttttt ctgttagttt
    23881 ctcagcaaat tcatttgctt gttcaactgt tccttcatca cctgccaaga tcgttacgat
    23941 ctctgtgtcc tcattgatca tttgttctag tgtacttaac gctgttgctg tgatatctgg
    24001 gttagagatc aggatttttc catcgatcat gcccaaaaag tctccttctt tgatctctac
    24061 accgtcaatt gaggtatctc tgatcgcgtg agtgacttga ccgctcacaa cgctagacaa
    24121 catttctgtc atcgtttctt tgttttcttc taaagaagct tgatcattga atgaaagcat
    24181 agctgtcaat ccttgggaaa tcgttcttga aggaacaact gctactggga tatctgctac
    24241 ttctgctgct tgatcagctg ccatgaagat gttcttattg tttggcaaga caatcacttt
    24301 atctgcgttt acttcttgaa tcgctttaac aatgtcttcc gtacttggat tcattgtttg
    24361 cccaccactg ataacataat tagcgcctaa actttcaaac agctcttgga cgcctttacc
    24421 agcagcgatc gctacgatac catacgggat acgtggtgtt tcttttttcg caaactcgtt
    24481 cacttcttca tcgtgttcca aaatcgtctc atgctgcaag cgcatgttgt cgactttgat
    24541 tttgactaat gaaccgaatt tttgtccata attcaagact tctcctgggt tttctgtatg
    24601 cacgtggact ttgatgatct cgtcatcatt gaccacaagt aaagaatctc ctataccatc
    24661 taaatagtta cggaattctt cgtaatcgaa tgtactatcg actgtaggtc cttctccgat
    24721 ttttaccatg atttctgtac agtaaccaaa atggatatct tctgtcgcca tttgaccttg
    24781 gacactgcga tgatgttctg cattgaccat atcatccatt tctgctggag aaggttcata
    24841 tgtttcatcc gcttggaatt ctccattcaa tgcacttaaa aagccctcat aaacaaacag
    24901 cagtccttgc cctccactgt ctacgacacc gacttctttt aggactggaa gcaagtctgg
    24961 agttttagct aaagcacgct ttgccccttt tacaacagct gtcattactt caacgacatc
    25021 atcagtactt tttgctttct tttctccagc tttcgctgct tctctagcta ctgtcaaaat
    25081 cgttccttct actggtttca ttactgcttt atacgcagtt tcaactccat gagtgaaggc
    25141 tgcagcaaga tcggaagcat ttaatgagac aacatctgga atttgcttag aaaatccacg
    25201 aaacaattga gacaaaataa cgccggaatt tccgcgcgca cccatcagca atccttttga
    25261 aagcattgct gctaattctc ctactttttc tgaggttgaa tctactactg ccttagctcc
    25321 actagtcata gatagattca tatttgttcc cgtatcgcca tctggcacag ggaatacatt
    25381 taatgaattg acgtattctg catttacttg cagacgattc gcacctgcct ggaccatttc
    25441 ctggaactgg cttgcactta tttctgttac actcacctag ataatcctcc ttcagatcga
    25501 ctgctgagtt accagccagt ctcctgccta gtctggcagt acacgaacac cttgtacaaa
    25561 gacattaact gaattggcag ttacgcctaa taatgcctct agattgtatt tcactttttc
    25621 ttggacgttg cgggaaactt cagagatttt cgtaccatag ctgataatcg tataaacatc
    25681 tacagcaata ccattatctt cttggcggac aactactcct cttgagtaat tttctttacg
    25741 caaaatctca gtaatattat ctttaatttg atttttgctg gccattccta caacaccgaa
    25801 cacatctgtc gcggcgccac caacaactgt tgcaattaca tcattggtaa tttcaatggt
    25861 tccagctgac gtttgaattt ttacagccat gaatgagcct ccttaacggg taaaaaactc
    25921 ccatttttct tctatcatat tttatcatag cgcaacttga aataaaaggc agttacccaa
    25981 agtactcaaa aaccttgcaa aatttcaggt ataaccttgt ttaatttaca attattcttc
    26041 tcaattgtca attgtatcta tgaaaatgta cttgcaaatt tccattctat atgatatgtt
    26101 agtctggtat gagctgaatt attctattgc tccaaatcaa ggcaaaggag gaaattacca
    26161 tggcaaaagt ttgttacttt acaggtcgca agacaaaaag tggaaacaac cgttcacacg
    26221 ctatgaactc aacaaaacgt actgttaaac ctaacttaca aaaagttcgc gttatggtcg
    26281 acggaaaacc taaaaaagtt tgggtgtcaa ctcgtgcttt gaaatcagga aaagttgaac
    26341 gcgtttaatt gaatgatact tggaccgttc agatagtgaa cggtttttt
//
LOCUS       DACCXW010000029        25495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCXW010000029 DACCXW010000000
VERSION     DACCXW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.029"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            126..3653
                     /locus_tag="IVY71_000990"
     CDS             126..3653
                     /locus_tag="IVY71_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7985057.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="IVY71_000991"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="IVY71_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7985058.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="IVY71_000992"
     CDS             7441..7782
                     /locus_tag="IVY71_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7985059.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="IVY71_000993"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="IVY71_000993"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7985060.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="IVY71_000994"
     CDS             9139..11559
                     /locus_tag="IVY71_000994"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7985061.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IVY71_000995"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IVY71_000995"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7985062.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="IVY71_000996"
     CDS             12993..13730
                     /locus_tag="IVY71_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7985063.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="IVY71_000997"
     CDS             13744..14559
                     /locus_tag="IVY71_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7985064.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="IVY71_000998"
     CDS             14572..15219
                     /locus_tag="IVY71_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7985065.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="IVY71_000999"
     CDS             15235..15891
                     /locus_tag="IVY71_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7985066.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="IVY71_001000"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="IVY71_001000"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7985067.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="IVY71_001001"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="IVY71_001001"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7985068.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="IVY71_001002"
     CDS             18233..18751
                     /locus_tag="IVY71_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7985069.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="IVY71_001003"
     CDS             18858..19358
                     /locus_tag="IVY71_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7985070.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19638..20558)
                     /locus_tag="IVY71_001004"
     CDS             complement(19638..20558)
                     /locus_tag="IVY71_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7985071.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20723..21736
                     /locus_tag="IVY71_001005"
     CDS             20723..21736
                     /locus_tag="IVY71_001005"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7985072.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21846..22601)
                     /gene="fabI"
                     /locus_tag="IVY71_001006"
     CDS             complement(21846..22601)
                     /gene="fabI"
                     /locus_tag="IVY71_001006"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7985073.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22821..23261
                     /gene="fabZ"
                     /locus_tag="IVY71_001007"
     CDS             22821..23261
                     /gene="fabZ"
                     /locus_tag="IVY71_001007"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7985074.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23376..24137)
                     /locus_tag="IVY71_001008"
     CDS             complement(23376..24137)
                     /locus_tag="IVY71_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7985075.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24326..25420
                     /locus_tag="IVY71_001009"
     CDS             24326..25420
                     /locus_tag="IVY71_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7985076.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4423 c         5383 g         7241 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgagcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
    19501 tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    19561 tagagcgtgg gacaaaagtg ttctttactt ttgtcccacg ctctttttag tgagattcgt
    19621 tgcatcgttt gtccaaatta ttttccgaca gcaattggac tgttcggaat cgtgaaacca
    19681 gaagctgtca attctttgac ataggagctc aagaactctt cttttaaggc atattgtttt
    19741 ccgttcaatg cgtagcagac cgtacgaatc gcaaaattgc tgtttccaag atccactaat
    19801 ccaaagatag aaggttcagt ttggagctct tcttggtatt tttcagctaa tgtacgattt
    19861 actcgatcaa tgatctcata gattttgtca tatccttctt caggaacaat ccgaatatct
    19921 aaaagtactt gcatatttgc acgagagaga ttgctgatcg tggtgatgtt tcgattcggg
    19981 atgaaatgga cagtaccatc cgttgctttc agctgaaggg tacggatacc aacagatacg
    20041 acggttcctt caatcgttaa gtttgctagc ttgatgtagt ccccaacatc catctgttgt
    20101 tccatgataa taaaaaatcc ggtaataaca tcactcatga acccttgggc accaagaccg
    20161 attgccacac ctgcaattcc ggcaccagcc aaaagtgatc cgatcggcac accgatagca
    20221 gataaaattg catagataaa aaagaaacca agggtataat gaaaaatagt atttaaaaga
    20281 ctatgtaaag tctttaagcg gctctcgttt agaatggctt ttttagaata ctgattaaaa
    20341 gacttatcaa tcagatattt tcccacacgg tgaagaatcc aaaaaaggac gatcaaaaaa
    20401 atcagatata aacttttttg gataaaggca gccaaaatgg cgtcccaatc aatcccgtcc
    20461 caaaaccgct gaaacgcatt tactttgctg acagcttgat ctgtcaatgt ctcgccagaa
    20521 gagtctgtcg taccggcagt tgctaataat gaaaacataa aatcctcttt tctaaaattt
    20581 gattctttct gtattgtacc aagaagtttt caaaaaagga aatagcaaaa ataactataa
    20641 tgaaggaaaa aagctgaaaa taaaatgaca aataatatgg ataatgataa aatgtggaga
    20701 aaataacgaa ggtggtaagt atatgcaaat cgattggaac aatctaggtt tttcttatat
    20761 caaaactcct tggcgtttta ttgccaaatg gaaagacgga caatgggaag aaggagaatt
    20821 gacagaagat aattacctga cgattcatga aggctctcca gcgcttcatt atggtcagca
    20881 atgttttgaa ggtctaaaag cttatcgtcg aaaagacggc aaggtcaatc tctttcgacc
    20941 tgaacaaaat agtcgacgga tgaatcaaag tgctagacga ttgctgatgc caatggtccc
    21001 tgaaaagatg ttcgttgatg ctgtcaagca agtggtaaaa gcaaatgaag attttgtgcc
    21061 gccctatgga acaggcgcta cattatactt gcgcccttta ctgattgggg tcggtgaaaa
    21121 cattggtgtc catccagctc cagaatattt atttgtcgtc ttctgtacgc cagtaggagc
    21181 gtatttcaaa ggtggactaa aaccaacgaa ttttattgta tctgattacg atcgtgctgc
    21241 tcctaagggg actggtgcag caaaagtcgg cggaaattac gcagcgagtt tacttccagg
    21301 aacagaagca aaagaactgg attactcaga ttgtgtttat cttgatccag ccacacatac
    21361 gaaaatcgaa gaagtaggtg cagcaaattt ctttggaatt acaaaagacg gaaaattcat
    21421 cactccgcaa tcgccctcta tcttaccaag tatcacgaaa tattccttgt tgcagttagc
    21481 tgaagaacga ctaggactgc aagcaattga aggagatatt ttcattgatg atctagatca
    21541 atttgcagaa gctggtgcat gtggtacagc ggctgttatt tctccaatcg gtgggattta
    21601 tcacaataac aatcttcatg tattttatag tgaaacggaa gtaggaccag taacaaaacg
    21661 gttgtatgat gaattgacag gtattcaatt tggagacata gaagcaccag aaggctggat
    21721 tgaagtcgtg gaataatttc aaagtaatga gttgaaaaaa acgtaaaaag aaaaaatcag
    21781 gaagagactt taaaaagttt tttcctgatt tttatatgtt ttactttaaa gaatgcaaca
    21841 aaagtttatg aaagatggac acctttgtca acgtagataa tatcaccgat gattccgctg
    21901 gaaagtgggc tgattaagaa agcacaagca ttaccgactt cttcgatcgt cacaccttgc
    21961 tgatcaggcg tacgagattc agataactgg atcagttttt gatagtcttt aacacctgtg
    22021 acagctaatg ttttgattgc cccagctgag attccattga cccgaattcc tttaggcgag
    22081 aactcagctg ccaaataacg gatagcagct tcaagagaag ctttcgcaat acccatcatg
    22141 ttataattcg ggattgcgcg ttctgaacct agataagtca tgctaacgat cccgctgttt
    22201 ggtgcaagaa acggttgtgc gtattttgat acagcaatta aagagtagct gctgatatct
    22261 tgagctaatt gatagccgtc tctggaaata tcagacacat ttccggacaa ttcttctttg
    22321 ttggcgtaag caactgcatg gacaaggccg tggatctccc cagcatattc accgatggca
    22381 ttcattgctt tttcgatgct ttcgtcgcta gaaacatcac attctacagt gaatgcttct
    22441 tcgccgacca atttgactaa tgattttttc attcgttcat tttggtaagt atagataatt
    22501 tctgcccctt gtttcaacat tgcttcagca caaccccaag caatgctttt tttgttagcg
    22561 acgcccataa tgacgatttt ttttccttct agaaatgaca taatgactct cctttgtttc
    22621 tgataaaaat ttgatagtcg taaaaaaata gagaataata gctaaaataa cttcttgatt
    22681 tcttaattat tagtcaaaat gttttgattg tcaaactatt ttttcttaaa ttaaaaaaag
    22741 attgctaaat atttaggaat atgatacttt gatagtcaaa gtaatgaaaa tggcaattgc
    22801 ctgaaagaag gagtttaatt atgtcttacg tattatctgc tactgaagta atggaattga
    22861 ttcctaatcg ttatcctatt tgttatattg attatgtaga tactttagaa ccaggaaaaa
    22921 agatcgttgc gacaaaaaat gtaacaatca atgaagattt tttccaaggt cactttcctg
    22981 acaatccagt gatgccgggt gcgttgattc tagaaacact ggcacaagca ggttcgatac
    23041 ttatcctaaa atcagaagag tttctaggaa aaagagctta tatcggtggg atcaataagg
    23101 ccaaattccg tcaaaaagtt gttccaggag atgtgctgaa atgtcatttt gaaatcacca
    23161 aaatgaaggg atcagtcggt acagcgttat ctgcagcata tgtagatgat aaaaaagtct
    23221 gtgagtgcga gttcacgttc atcgtcaacc aaaatgcttg actataaaac ctgatatagc
    23281 aaaacaaaag gaagcttact tgctttttat gtaaaaagaa gacgcctgaa tcctagcata
    23341 cgttgtcatc tatgcaggat tcagacgtta tttttttatt tttgttttgc ttcttgcagt
    23401 atattgattt tatcgatttt tacttcttct tttggtttgt ctgaactatc tgtttcgata
    23461 tttgcaatag catccacgac atccattcct tcaatcactt ggccgaagac agtataatgg
    23521 ccatctagtg aaggataacc gccgttttta taagcgtcga tgattgcttg aggatagtcg
    23581 tcttttaaaa gaccatcatg catatcatct gtattttgaa cgataaagaa ctggctgccg
    23641 ttactgttag gatcttgtga gcgagccata gataacgcac ctcttatatt ataaaggtgg
    23701 ttgttgatct ctgtttcaaa tccttctccc caaatgcttt cccctccagt gccatcgcct
    23761 ttaggatcgc caccttggat catgaaatct ttgatgacgc gatggaaagt cagtccgttg
    23821 tagtaaccgt cttttgcatg tgtcataaag ttttcaaccg cttttggtgc gtgttcggga
    23881 aataatttga ttttgattgt gcctttattc gtcaccattt cgaccagatc ttcattttcg
    23941 gcgacatctt tcgtcaattg tggtaattcc aatgcgttca aatcgacgct actggcagtc
    24001 gtgctttccg cagtagatgt actttttgaa ctttctgtag acgaactaga agaagtcgtg
    24061 ttgtttgcag aacaaccagc agcaaacagc aatgctgctg ctaaactaat acttaaaata
    24121 actttatttt tcttcataac tgttcctttc caatttacta ttttgttatc tttaatgata
    24181 acaaaataga gggaaaatac ctacttaaat tattggaatt tatcatttat tttaaaagaa
    24241 aaagaaaagg tgtagaccat ttactttgtg tttgctataa tgtaagtgga tacaaaaacg
    24301 aaaagatgca ggaggaaatg gtaatatggg aaaattagga atctcgattt atccagaacg
    24361 gtcaacattt gaaaaggaca aagcgtactt ggatttagct cataagtatg ggtataaacg
    24421 ggtttttacc agtttgctgc agatcaatga tgataaagaa aaagttcttt cagaatttaa
    24481 agaagtagtt gattacgcga atagcctagg catggaagta atggttgata tcaatccagc
    24541 gttatttgaa cagttggaga tttcttacga tgacttatct tttttccata agatgggtgc
    24601 ctacggggtc cgcttagata tcggattcac aggagcagaa gaagcaaaaa tgacgcgcaa
    24661 tccttttggt atcaaaatcg agatcaatat gagttcgggc acgaattacg ttgacaacat
    24721 catgagttat tcgccaaaca cggataattt attaggttct cataattttt atcctcatcg
    24781 atactctggt ttaggatacg aacactttgt tttttgttca gaaaaattca gaaaatataa
    24841 tttgaatacg atggcattcg tcaattcgca atctgctgag tttggccctt ggcctacgca
    24901 agatggcttg tgcacgctgg aagaccaccg agatcttgaa attgcgacac aagtgaagca
    24961 tttaatcctt acaggattga ttgatgatat ttctattgga aatgcgtacg caagtgaaga
    25021 agaattgaaa gaaatggcag aagcatttaa cgcggattat ccaacattga aagtagatac
    25081 ggaagaaggt attacagaaa acgaacgaat ctgcttgttt gataaccttc atagttatcg
    25141 aggggatcgc tcagaatata tccttcgttc aacgatgact cgtgtatact acaaagataa
    25201 agatttcccg cctcataata cgcgagatat gcatcatggg gatgtactga ttgacaatga
    25261 agggtacggt caatacaaag gagaaacaca aatcgccttg aaggatatga aaaacgacgg
    25321 acgtgtcaat gttgtaggaa gaatctccga tgacgaatta ttcctccttg actttttgaa
    25381 accatggtca agctttaaat taattgaaaa taaaaaatag ataattagtt attgcagaaa
    25441 taatccgcta gaaatagcca gttatttctg cttttttatt ttgttttttt atagg
//
LOCUS       DACCXW010000030        24390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCXW010000030 DACCXW010000000
VERSION     DACCXW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.030"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..616
                     /locus_tag="IVY71_001010"
     CDS             <1..616
                     /locus_tag="IVY71_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7985077.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="IVY71_001011"
     CDS             613..1386
                     /locus_tag="IVY71_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7985078.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="IVY71_001012"
     CDS             1383..1781
                     /locus_tag="IVY71_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP7985079.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="IVY71_001013"
     CDS             1966..2637
                     /locus_tag="IVY71_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985080.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="IVY71_001014"
     CDS             2696..3136
                     /locus_tag="IVY71_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985081.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="IVY71_001015"
     CDS             3148..3816
                     /locus_tag="IVY71_001015"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7985082.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="IVY71_001016"
     CDS             3880..4503
                     /locus_tag="IVY71_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7985083.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="IVY71_001017"
     CDS             4559..4945
                     /locus_tag="IVY71_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7985084.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..6549
                     /locus_tag="IVY71_001018"
     CDS             5158..6549
                     /locus_tag="IVY71_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7985085.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7160..7528
                     /locus_tag="IVY71_001019"
     CDS             7160..7528
                     /locus_tag="IVY71_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7985086.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7708..8649
                     /locus_tag="IVY71_001020"
     CDS             7708..8649
                     /locus_tag="IVY71_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7985087.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8718..9449
                     /locus_tag="IVY71_001021"
     CDS             8718..9449
                     /locus_tag="IVY71_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7985088.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9593..11773
                     /locus_tag="IVY71_001022"
     CDS             9593..11773
                     /locus_tag="IVY71_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7985089.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11793..12275
                     /locus_tag="IVY71_001023"
     CDS             11793..12275
                     /locus_tag="IVY71_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7985090.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12291..13052
                     /locus_tag="IVY71_001024"
     CDS             12291..13052
                     /locus_tag="IVY71_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7985091.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13183..14517
                     /locus_tag="IVY71_001025"
     CDS             13183..14517
                     /locus_tag="IVY71_001025"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7985092.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14824..15309
                     /locus_tag="IVY71_001026"
     CDS             14824..15309
                     /locus_tag="IVY71_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7985093.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15302..15985
                     /locus_tag="IVY71_001027"
     CDS             15302..15985
                     /locus_tag="IVY71_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7985094.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16111..18756
                     /gene="polA"
                     /locus_tag="IVY71_001028"
     CDS             16111..18756
                     /gene="polA"
                     /locus_tag="IVY71_001028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7985095.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18801..19637
                     /gene="mutM"
                     /locus_tag="IVY71_001029"
     CDS             18801..19637
                     /gene="mutM"
                     /locus_tag="IVY71_001029"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7985096.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19601..20230
                     /locus_tag="IVY71_001030"
     CDS             19601..20230
                     /locus_tag="IVY71_001030"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7985097.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            20282..20614
                     /locus_tag="IVY71_001031"
     CDS             20282..20614
                     /locus_tag="IVY71_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7985098.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20926..21417
                     /gene="nrdR"
                     /locus_tag="IVY71_001032"
     CDS             20926..21417
                     /gene="nrdR"
                     /locus_tag="IVY71_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7985099.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21440..22813
                     /locus_tag="IVY71_001033"
     CDS             21440..22813
                     /locus_tag="IVY71_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7985100.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22813..23739
                     /gene="dnaI"
                     /locus_tag="IVY71_001034"
     CDS             22813..23739
                     /gene="dnaI"
                     /locus_tag="IVY71_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7985101.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23868..24308
                     /locus_tag="IVY71_001035"
     CDS             23868..24308
                     /locus_tag="IVY71_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7985102.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         8160 a         4172 c         5374 g         6684 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     6181 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     6241 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     6301 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     6361 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     6421 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     6481 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     6541 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     6601 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     6661 ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     6721 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
     6781 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag
     6841 agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt
     6901 tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt
     6961 tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa
     7021 acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag
     7081 ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
     7141 atcgtttagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt atgtggacga
     7201 tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag atcgtcaaga
     7261 aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct catttctgtt
     7321 atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg ccccatcttt
     7381 gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc aatcagtcac
     7441 attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg atcccgatgg
     7501 caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa
     7561 caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa aactttccat
     7621 gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta tactgtttag
     7681 gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta ttacaattat
     7741 acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg caaatctttg
     7801 gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt gtctggaacc
     7861 gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt tgctgatacg
     7921 atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt aagataccgt
     7981 ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt gacaggtgcc
     8041 tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt ggtgattgat
     8101 gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg cccagatata
     8161 cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt tatggaaaaa
     8221 cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg ttggcttgtc
     8281 cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta tggtaaggat
     8341 cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga acggatcgct
     8401 gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga cggaacacca
     8461 tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct tgcttccatt
     8521 gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat
     8581 cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt tacagcttta
     8641 attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct atactagcgt
     8701 tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc catggcaaac
     8761 atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag cttgttttat
     8821 taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta ggaaagcaat
     8881 tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat tttttgcaat
     8941 ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga aaagaaacgt
     9001 ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa atctcgatga
     9061 tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc ttgtattatg
     9121 aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa gactggcgta
     9181 agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg aaaaacaata
     9241 caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga gatatgcaga
     9301 caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct gtattcggag
     9361 ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt gaatatcaga
     9421 acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag ctgtgacttt
     9481 gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt aacccaggat
     9541 aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt taatgacgga
     9601 aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa
     9661 gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg
     9721 ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg
     9781 gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc taatcgatga
     9841 acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga aaatgcaaca
     9901 agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa cgattgctaa
     9961 agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg gagaggtgca
    10021 atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt
    10081 gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg
    10141 gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc
    10201 agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc ataaaatggt
    10261 ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga aagtattctt
    10321 tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca aaaatcctgc
    10381 ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac gctttattca
    10441 gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg agcaagcaat
    10501 cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt
    10561 tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac
    10621 agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc aagcaaaaag
    10681 agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag atatggtagc
    10741 tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc agttgaaatc
    10801 cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg tttactccgc
    10861 tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa gtgctgtttc
    10921 tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc ctaaagctgt
    10981 cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac aattggattt
    11041 tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa gcccgcagct
    11101 cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca tttatcgtga
    11161 agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct taggaccaaa
    11221 agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca ttttggacaa
    11281 cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt cagtccaact
    11341 ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat tatccgtaga
    11401 gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt tagctgggct
    11461 gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac gtacagatgt
    11521 attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc gtaatgtcat
    11581 tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc atatatcgaa
    11641 actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag atatcgtaac
    11701 agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga tgctttcccc
    11761 ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg cagcagttag
    11821 tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca atgttcaatc
    11881 cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta gattttaatg
    11941 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
    12001 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
    12061 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
    12121 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
    12181 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
    12241 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
    12301 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
    12361 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
    12421 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
    12481 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
    12541 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
    12601 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
    12661 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
    12721 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
    12781 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
    12841 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
    12901 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
    12961 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
    13021 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
    13081 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
    13141 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
    13201 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
    13261 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
    13321 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
    13381 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
    13441 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
    13501 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
    13561 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
    13621 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
    13681 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
    13741 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
    13801 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
    13861 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
    13921 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
    13981 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
    14041 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
    14101 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
    14161 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
    14221 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
    14281 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
    14341 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
    14401 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
    14461 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
    14521 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
    14581 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    14641 caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta tttgatgcag
    14701 gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg gagcagaact
    14761 tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg gagggtttct
    14821 atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc
    14881 gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta tttgggattg
    14941 acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac agaatatcaa
    15001 cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat ttcaaagcat
    15061 cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga accggttttc
    15121 cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat gaaggatgtg
    15181 gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt
    15241 atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg
    15301 aatgaataat ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg
    15361 atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc tttgggtatt
    15421 tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg gtctgtggat
    15481 cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc cgagcttagc
    15541 aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg ttactcttgc
    15601 atactacgat attggaacag taacgcaagc tttcttgaca gctactttga tgttcatggg
    15661 gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa gagcaggaca
    15721 ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt tgagaagtag
    15781 cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga ttacagctta
    15841 tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac atagtggaat
    15901 tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct tggcgtttct
    15961 gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg gctataccga
    16021 attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt gttagaatga
    16081 ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt actcgttgat
    16141 gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga acgtttcaaa
    16201 aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt
    16261 atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa aacgactttt
    16321 cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa
    16381 gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta ttacgaattg
    16441 cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga caaagatcaa
    16501 tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag taaagaagta
    16561 aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc agaacatgta
    16621 gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg actagccgga
    16681 gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta
    16741 ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc
    16801 aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg
    16861 accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta tgaagggaaa
    16921 aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt cctttctaaa
    16981 ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa
    17041 ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat gggcgacaat
    17101 tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat ttatgtcacg
    17161 aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga tccgactcgg
    17221 ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta tactggaaaa
    17281 acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa
    17341 agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca atcagatgaa
    17401 gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact cttttttgct
    17461 catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag ccaagaactg
    17521 gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc tatcattttg
    17581 gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga aatgaagggc
    17641 gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc cggcgaagaa
    17701 ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat gggattaccc
    17761 gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga acagcttcgt
    17821 gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa aattcaatct
    17881 acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca tacccgttat
    17941 gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt gcagaacatc
    18001 ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag agaaaaagac
    18061 tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc gcatatctca
    18121 aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc cagcacagcg
    18181 atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg taggcaggca
    18241 aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc acaaaatcta
    18301 ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa atacccaggt
    18361 gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg
    18421 ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa tatccgttct
    18481 tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga tattttgaaa
    18541 atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc tacgatgctg
    18601 ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga atcattagat
    18661 aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact gatcactgac
    18721 agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac agagcaatat
    18781 aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt cgtaaaggat
    18841 tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg cccaagatca
    18901 tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc atccaatcca
    18961 ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg atctcacact
    19021 tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat aaacatacgc
    19081 atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta cgaaaatttg
    19141 gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg
    19201 gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga ctgaaaaagt
    19261 cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt ctaggtaata
    19321 tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg gcgaacacac
    19381 tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg gatcgtgcaa
    19441 tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa tcaggcggat
    19501 ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc
    19561 cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc
    19621 taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta ccggtaaaag
    19681 tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg atgtgattgc
    19741 cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag tgtttggcca
    19801 agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga ttgtttttcc
    19861 atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc gaaaagcgat
    19921 aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag atatcccatt
    19981 gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt atactacgcc
    20041 agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac aagcgcagca
    20101 gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata ttttgttcga
    20161 caacaatgga acaaaagaaa aacttgtggg acaaatcgaa aattggcttt ttgctaatcg
    20221 ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt gagggatagc
    20281 gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg
    20341 cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag aaacagcaga
    20401 agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc aggcagaaga
    20461 agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa
    20521 aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg aacagcgaat
    20581 gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga gatgtgactt
    20641 gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga ctaaatcagc
    20701 ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt gaaatttagt
    20761 aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt
    20821 ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga
    20881 aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag atgtcccaga
    20941 tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg acgtgcgatc
    21001 cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga acgaattgaa
    21061 gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa
    21121 attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga acaaatggaa
    21181 caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg
    21241 aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat cgcttatatt
    21301 cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa
    21361 gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa caattaagaa
    21421 acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa aaaatattta
    21481 ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc tgtatcttta
    21541 tcagccacta atcggcggag aagccttgag tatctatttc accctgttat cagaaatctc
    21601 tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta gtctaggctg
    21661 cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct tactggatgt
    21721 ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt tagaaccact
    21781 cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg aaaaagttgg
    21841 tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata cagcagaggg
    21901 atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc
    21961 tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg aatcagtagc
    22021 attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc tggcaaaaaa
    22081 acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc atcagcttta
    22141 cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact ttagtgaaca
    22201 aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac gtccggtgaa
    22261 gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt caaacgaaca
    22321 tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac cgatgaaata
    22381 tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa actggttact
    22441 gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt tgctgaatta
    22501 tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata cagtggcaaa
    22561 tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca ttcggcaaat
    22621 aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg
    22681 aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat
    22741 cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg caaaaaagga
    22801 gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat
    22861 tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga tgttcaagct
    22921 tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag ttacgctaaa
    22981 ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct
    23041 cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac ttatgtacca
    23101 acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg
    23161 gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac agatggacga
    23221 ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga tccaaaaggg
    23281 tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata tttattagga
    23341 gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca ttttccgaca
    23401 tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa gttagatgca
    23461 gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat gagtagttgg
    23521 atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca gttaccaacc
    23581 ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt cactcaacgt
    23641 ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta tttgacaaaa
    23701 gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa gtccaactga
    23761 catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa atgaccggag
    23821 acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg agggctattt
    23881 tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt agcgcagata
    23941 gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca agcagtacga
    24001 aaacggagag acaaagcata tttgtcggaa cgattgaaag tatcactcaa gtagaagcag
    24061 aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat ccagcgaata
    24121 tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa aaaataagcg
    24181 tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt gagatgccga
    24241 tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat gtttcattga
    24301 acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg
    24361 tatatttttt aaggagatat gggaattttt
//
LOCUS       DACCXW010000031        24346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCXW010000031 DACCXW010000000
VERSION     DACCXW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.031"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..822)
                     /locus_tag="IVY71_001036"
     CDS             complement(<1..822)
                     /locus_tag="IVY71_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7985103.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAI"
     gene            complement(1046..2986)
                     /gene="thrS"
                     /locus_tag="IVY71_001037"
     CDS             complement(1046..2986)
                     /gene="thrS"
                     /locus_tag="IVY71_001037"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7985104.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3323..3502)
                     /locus_tag="IVY71_001038"
     CDS             complement(3323..3502)
                     /locus_tag="IVY71_001038"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7985105.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3549..5372)
                     /gene="cadA"
                     /locus_tag="IVY71_001039"
     CDS             complement(3549..5372)
                     /gene="cadA"
                     /locus_tag="IVY71_001039"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7985106.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5650..7929
                     /locus_tag="IVY71_001040"
     CDS             5650..7929
                     /locus_tag="IVY71_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7985107.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8022..8669)
                     /locus_tag="IVY71_001041"
     CDS             complement(8022..8669)
                     /locus_tag="IVY71_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985108.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8848..9567)
                     /locus_tag="IVY71_001042"
     CDS             complement(8848..9567)
                     /locus_tag="IVY71_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7985109.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9698..10849)
                     /locus_tag="IVY71_001043"
     CDS             complement(9698..10849)
                     /locus_tag="IVY71_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7985110.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11066..11806)
                     /locus_tag="IVY71_001044"
     CDS             complement(11066..11806)
                     /locus_tag="IVY71_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7985111.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11810..12133)
                     /gene="rsfS"
                     /locus_tag="IVY71_001045"
     CDS             complement(11810..12133)
                     /gene="rsfS"
                     /locus_tag="IVY71_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7985112.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12190..12783)
                     /locus_tag="IVY71_001046"
     CDS             complement(12190..12783)
                     /locus_tag="IVY71_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7985113.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12767..13414)
                     /locus_tag="IVY71_001047"
     CDS             complement(12767..13414)
                     /locus_tag="IVY71_001047"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7985114.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13427..13738)
                     /gene="yhbY"
                     /locus_tag="IVY71_001048"
     CDS             complement(13427..13738)
                     /gene="yhbY"
                     /locus_tag="IVY71_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7985115.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13754..14863)
                     /gene="yqeH"
                     /locus_tag="IVY71_001049"
     CDS             complement(13754..14863)
                     /gene="yqeH"
                     /locus_tag="IVY71_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7985116.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14860..15387)
                     /locus_tag="IVY71_001050"
     CDS             complement(14860..15387)
                     /locus_tag="IVY71_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7985117.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15567..16352)
                     /locus_tag="IVY71_001051"
     CDS             complement(15567..16352)
                     /locus_tag="IVY71_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7985118.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16345..17214)
                     /locus_tag="IVY71_001052"
     CDS             complement(16345..17214)
                     /locus_tag="IVY71_001052"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7985119.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17215..18594)
                     /locus_tag="IVY71_001053"
     CDS             complement(17215..18594)
                     /locus_tag="IVY71_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7985120.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18602..19021)
                     /gene="fabZ"
                     /locus_tag="IVY71_001054"
     CDS             complement(18602..19021)
                     /gene="fabZ"
                     /locus_tag="IVY71_001054"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7985121.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19046..19522)
                     /gene="accB"
                     /locus_tag="IVY71_001055"
     CDS             complement(19046..19522)
                     /gene="accB"
                     /locus_tag="IVY71_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7985122.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19525..20760)
                     /gene="fabF"
                     /locus_tag="IVY71_001056"
     CDS             complement(19525..20760)
                     /gene="fabF"
                     /locus_tag="IVY71_001056"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7985123.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20779..21516)
                     /gene="fabG"
                     /locus_tag="IVY71_001057"
     CDS             complement(20779..21516)
                     /gene="fabG"
                     /locus_tag="IVY71_001057"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7985124.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21528..22445)
                     /gene="fabD"
                     /locus_tag="IVY71_001058"
     CDS             complement(21528..22445)
                     /gene="fabD"
                     /locus_tag="IVY71_001058"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7985125.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22552..22776)
                     /locus_tag="IVY71_001059"
     CDS             complement(22552..22776)
                     /locus_tag="IVY71_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7985126.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22829..23791)
                     /locus_tag="IVY71_001060"
     CDS             complement(22829..23791)
                     /locus_tag="IVY71_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7985127.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23801..24241)
                     /locus_tag="IVY71_001061"
     CDS             complement(23801..24241)
                     /locus_tag="IVY71_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7985128.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6655 a         5427 c         4242 g         8022 t
ORIGIN      
        1 gattgcttct cgtgtttttt tcactttttc gctaagcttt tcgtttttag ccatatagaa
       61 ataaaaaatg atatcgatcg tagcaaattg ggccaaaagt gaattagttg ccgcacttcg
      121 attcgtgatt tcttttggtc tagatgtttg tacgagtaca tcggctatct tgctaagcgg
      181 gttatttcca aattgcgtca acgcaatgat ttcgatattc atgcttttcg ccagctcggc
      241 taatgcgact acatctgcac ttctacctga gtttgaaaca agccaaagca ctttcttttt
      301 ctcattgctg acaagttgcg ggagcaagac gtggatatct ttttcaaaaa tgattggctt
      361 accgacacga gaccattttt gcagaatatc ttctactact aaagaagagg cgccaacccc
      421 atataaaaaa acagtctctg ccgattccaa acactctacg actttttgga tcgtttcttc
      481 ttctagaata tctactgttt cttttatcgc tacttgtgca ttgttaccaa gtttgttttt
      541 gatagagtaa aaggactcat ttgcttctat atcactaaaa ccttcatcga ttccagtctg
      601 tagttctgtt gaaatcgtga ttttgaagtc cgtcagactt tgaaaaccga tttttttgga
      661 aaaacgtaca actgtcggcg cactcaaccc ggaagcttct gctaattcat tagctgtcat
      721 tgtagggacc ttctcaatat taccgataat gtagttggcc aacttttttt ctgctgaaga
      781 ataatcttcc atcatacttt caatacgact aattacgctc ataagttcct ccaccctttt
      841 aatgatagtc tatgtaagta taccatatca gacccttatt cacggtcgta tactggataa
      901 aaaacaaaaa aacaatccgc ccaacagaat atcttatccc atcaatccca cagattctat
      961 tgagcatctg tgtctttgag gaatacctgt tatctattgt tcggattgct ttctatctac
     1021 tactttttta caggaaattt acctattagt tttctctgct gtaatttttg acttctgctt
     1081 ccatagcatc aataaagata ttcaaatctt ctacagctgt ttctttactt ccataacgac
     1141 gtacgttcac tgttgcatct tccaattcct tgtctccaac aactaattgg taagggatct
     1201 tttgtgtttg agaggcacgt atcttatagc ccattttttc attacgatca tcaacttcta
     1261 cacgcaaccc cttcatttgc aaacgttctt tgatttcata agcataatcg ccatgtgcat
     1321 caacagaaac agggatgatt gttgcttgta taggtgccaa ccatgttgga aaagcgcctt
     1381 tgtatacttc tgttaaataa gcgacaaaac gttccattgt agaaacaatt ccgcggtgga
     1441 taactactgg acgatgcgta ttttccccgt cttctccaac atatgtcaaa tcaaagcgct
     1501 ctggcaataa gaagtctaat tgtatagttg ataatgtttc ttctgtacca agagctgttt
     1561 tgacttgaac atctagtttt ggtccgtaaa aggctgcctc gccttctgct tcaaagtaat
     1621 ctagttctaa ttcatccata gcggctttca gcattgcttg ggctttttcc cacattgcgt
     1681 catcatcaaa atatttatct gtattgtttg gatcgcgata gctcaagcgg aaacgataat
     1741 ctgtaatatt gaagtcagca tatactgcca ccatcaattc aagcgtacgt ttaaattcat
     1801 ctttgatttg atcaggacga acaaatgtat gaccatcatt caacgtcatt tctcgtacac
     1861 gttgtaaacc tgacaaagca cctgattttt catagcgatg catcattcct aattcagcga
     1921 tacgaattgg taattcacga taagaatgga tcgtgttttt gtagaccatc atgtgatgag
     1981 ggcagttcat tgggcgaaga acaagcattt cgccatctcc catatccatt ggcgggaaca
     2041 tgtcttcttg ataatgatcc cagtgtccag atgttttgta caactcaaca tctcccatta
     2101 ttggtgtata tacatgttgg taacctagac taacttcttt gtctacgata taacgttcga
     2161 tcgtgcgtcg gattgttgcc ccttttggaa gccagaatgg caaaccagaa cctacttctg
     2221 gggaaaccat gaacaagtca agttcttttc ctagtttacg atggtctctt tcttttgctt
     2281 cttcacggag acggatatat tctttcaaag ctttcttgtc aaaaaaggct gtcccgtata
     2341 cacgttgcat catttgattg tttgagtttc cacgccaata agcacctgcc actgataata
     2401 atttaaatac ttgtatacgg ccagtggatg gaacatgtgg tccacgacac aaatcgatga
     2461 attcgccttg ttgataagca gtaatcactt cttcttctgg caattcacta atcagttcta
     2521 ctttgtacgg atcacttgca aaaatttcta atgcttcctg ttttgaaaga acacggcgtt
     2581 cgatcggcag attttctttg acgattttca tcatttctgc ttcgattgcc ggcaaatctt
     2641 ctgcagttac ttggttttgt ccattatctg tatcgtagta aaaccctgaa tcgatagctg
     2701 gtccaacgcc aaagtggata tttggataga ggcgacgcat ggcttgggcc atcaaatgag
     2761 ctgctgaatg acggactaag ccaagtgcat cttcatggtc tggtgtgatg atctcaatac
     2821 ttgcatcttc ctcgattgga cgagtcaaat caacgagttt tccgttcact ttaccagcta
     2881 aagctttttt tgctaagctt tttgaaatgc tttcagcaat tgccaatgtt gttgttccag
     2941 actcaaattc tttgactgca ccatctggaa aagtaatttt tatcataata gtttcctcct
     3001 tgaaatgttt tgattgatca gatcatcaag ctgaaaaaca aaaaaatcct agcatctctt
     3061 gaaaagagat actaggacga aaagttcgtg gttccaccta atgttcaaac gcaaatgcgt
     3121 tcctcaagcg ttgtaacggt ccgaccgccc ttgcttcaac aagggtttcg aaagtggtca
     3181 tcctttggtt ttctcctgga aaagtttcag cctagctttt cctctcttat cgaagactct
     3241 caaattcagt tggctttctc atcaatcaat tatgtgttta tttaaccact aataaataga
     3301 aaaatcaagt gggaaactaa acttattttt tccattcccc atttacaccg tatgttcctt
     3361 ttttcatctc attgatgatg acatggatgt gttcttttgg tgcctgcgtg tttttatgaa
     3421 cagcttccgt gatatctttc atcatatttt ctaattgttc cggactgcga ccttctacta
     3481 attcgacatg tacgaatggc ataatgctct ctcctctttc tgatcgttct tttttcaact
     3541 cgattctatc atactatgtt tgtcttgggt actgaatcag gaaaaatggc tggatctttt
     3601 tttgctctca aggcactcaa aatagagacc gtatccacga cttcttgcaa aacagctccg
     3661 aataatgcgg ggattagtcc cgtacttgca attaacatca aaaaaataca aataaagatg
     3721 ccaatcagta cagactgttt cgctattctc atcgtatctc ttgaaatcac tactgctttt
     3781 gctacacgca agagatcatc tttcaagatg accgcatctg cactttcact agcagctgta
     3841 gaaccatgag cccccattgc aataccgata tctgcagcag caagtgctgg tgcgtcattc
     3901 acgccgtctc ctaccatcgc aacaggtctt tgatctttag ggatactttc taaaatggtc
     3961 agtttatctt gaggcaagct acgggcatgt acagaatcta attggacttt ttctgcaatc
     4021 gtttgagcaa tggcagatcc atccccggtg atcatcatca aatttttcac atcaagtttt
     4081 cttagctcct tgattgtctt tgctgcttct gggcgaatct gatccgaaaa taggatcgtt
     4141 cctgcaaaag atcgatcaat tgttacatag attgctgtat tggacgtttc tatttcttca
     4201 gctgaaacaa aagaggcacg tccaatcttt acttcatgat tattgactac tgcttggatt
     4261 ccttgaccgg tcacttcttt cagttgacta acaggataca gttggctgtt tttcacgttg
     4321 ttcactaacg aacgagctaa gatatggcta gattcctgtt ctgcactagc tgcatacaga
     4381 agtagctctt ctttttttat tccattgact ggctgaatcg cctctacttc caatatccct
     4441 ttggtaagcg ttcctgtttt atcaaaagca atcgtttttg tacgtgcgag cttttcaatc
     4501 gttgttccag tttttacaac gatcccgttt ttacttgaac ggctcattcc tgcaactaaa
     4561 gcaatgggcg cagctagaat cagtggacaa ggtgatgcca ctaccagtac ctcagcaaat
     4621 cgtaaagggt cttttgagaa ccaccaagaa ataccaccga tcaaataagc aatcaacgtg
     4681 aaagggactg cataacgatc agctaaacgg acaaatcggg caggcttttc ttttgattct
     4741 tcgactagtt taataatcat ttgatactgg ctgtctgcgg cacgtttttc agcgatcatt
     4801 tggatcgatc cgtctccatt taaagaccct gacataatct gatctccagg tttcttggtt
     4861 tccgggcggg actcacctgt caaagaagat tcatcgaccg aagattctcc tttggtcact
     4921 cgaccatctg cgggtacaag ttcgccaggc ttgactagta actggtcatc aatatttatt
     4981 tcatcgatta ctatatcctg caattttcct tctttgaatc gatgggcaat tcttggggtg
     5041 ttatctaaca aagactgcag ttctttaccc gctcgtttat tcgcgtaatc ctctagacta
     5101 tctccgcccg taagcatgat caatatcatc aaactagccc agtattcttt gacggctaat
     5161 gttgcaagga ttgcggtaat cgctaataca tctacgccat atcttcctga acgtaacgtc
     5221 ttgatcattt ccaaaaacat caagaaggcg agaatccctc cagtaaatgc gacaatcgca
     5281 aatgcaaggg ccggctggtg aaaaacaaat tcacttaata aagctatccc accaataagc
     5341 aaagtaagta aaaatttttt gaaatgtgtc attgtttttt ctcctctttt atgtgactta
     5401 gagaggttag taataaattt ccagctgcct ctttcatctt aagcatagtc cttgactagc
     5461 actttttaaa acaataggcc ttaattgaaa acaactatca ctatcagcgt ttccaaaatt
     5521 catcagttct acaaacaaaa atattcagca aggattactt tttcgattca tcagttaaaa
     5581 agtacctttt ttcgaaaaac tatgctatac ttttaattcg ttatttacgg cggaaggagc
     5641 aaacatgcga tgaatgagaa acaactagaa caaacgcacg tggatgaaac gatcgaacta
     5701 atccagatcg aacaaaatat gctggataca aaacaacaag aattgactca ggaaatgaat
     5761 cattcgacaa aagacacagc taatcacaag attcgagctg gttccaatga agctttttat
     5821 gaatctgctg ttgaatatcg ccaacatgag caggaattgc ttttgaaata ccatactctt
     5881 gaagcacagc aaaaacgcct gcaaacgttg tctgtcatga aagatagtcc ttattttgcc
     5941 cgtatcgact ttaaagaaga ccgtgaaaca gaaacattat atctaggtat cgcttcttta
     6001 agagatctgg cagaagaaac aatcgttatt gactggcgcg caccaattgc caatctttat
     6061 tatgaaggtg agattggtcc cgcattttat gaaactgaaa cagatagaat agatgtcgat
     6121 ctgcagttga aacgacaatt taaaatccaa gaaggacgta ttttatctat ggtagatacg
     6181 tcggaagtaa tcaatgatga ctttttgcta gagatattgg atgaatcatc aagcaaccat
     6241 atgaaaaaca tcgtctctac gatacaaaaa gcacaaaatg caatcattcg ggacacaaca
     6301 agtaaagtaa tgctgattga aggaatcgcc ggaagtggaa agacatccgc tttgcttcaa
     6361 cgtattgctt ttctgttgta tcataatcgc aagtggctcg aggctgaaaa cgttcttcta
     6421 ttttcaccca accatttgtt ttctgattac atctcgactg tattgccttc attaggtgaa
     6481 agcggcgtgc ctacacagac attcaaaaat tatttggtgc agctgctgcc agagttttct
     6541 ttactagatg aacagcaaca agaaagtggt tttttgctag gggaaaaaga tccgatacaa
     6601 gtgatgaaaa gcggattgac cttagtcgat cagattgatc gctatatcca aggcatcaca
     6661 aactacggtc ccttgttccg tgatatgaaa ataaatggcc gtacgatcct atcaaaggaa
     6721 tcgattcgcc aatggtataa agaaacaaat gaacaattac ctttacatca tagactttct
     6781 ctattacaaa cgaagctcct gaaaaaatta ggcggtctgc aaaaagacga aacacgtcag
     6841 caatgggtca aagatctggc ggaagaacag ctccaagaac tatacgcaac agatccaaat
     6901 cttgaatata cagaacaaaa ggagcgttca ttacgtaaaa aactggccaa ccaaatcgta
     6961 cgtaaaagat tccgtcgaat ccatcgaggg atcatgaatt atcaatttgt caatataacg
     7021 aaacaatatc tccactttct acaaactgtg ccactttctc ttctaacacg acataaaatc
     7081 agcgagacca ggtggcagga acatttgaca actgttcgta cacacttgcg tcaaagagaa
     7141 ttgcgtcaag aggatgctgt actattcttt ttactgatgc gccgaacata tccagtgacg
     7201 gttacacaca aagctcgcta tatctttatt gatgaaatgc aggatttcgc accatcacaa
     7261 gtggcattgc tgcaagcctt gttcccaaaa gcaaatttga ctttctgtgg agatctaaat
     7321 caaaatgtct ttggaaatga aacgatcgtt ggatcattgg aagacctttt cgtggaccgt
     7381 gaagtcactc gtttccaatt aacgacaagc tatcgctcca caaaagaaat cactgatttt
     7441 gctaatcatt ttattgtgaa agaacatcaa gtagaaacaa cagcaagaaa aggagacctt
     7501 cctttgctca tccaaggaac aacagatgat atcaaattga attggttagc agataccatt
     7561 cgaacgactc agggaaaagc ccggtattgg cgtacagcaa tcatagctaa aacacaagaa
     7621 gaatgcgaag ctctttatgc tcaactgcca gacgagttga aacaacaagt ccagctgatt
     7681 ttaaatgaga gtgactttat gaagcgttcc attattttac ttccagcctt tttagcaaaa
     7741 ggtctggagt ttgaccgagt tttcttgtgg gatgtagatg cagaacattt ttcttctcag
     7801 caagacaaat tgatccttta caccatgtgt acacgtgcga tgcatgaatt gacccttttt
     7861 acctccaata atgaaagtcc tttgttaaac aatatggata tttcatctta tgagaaaaaa
     7921 gtactgtaag taaaaagaca actgctatca ttaatgttag cagttgtctt tttatttaca
     7981 ataataattg cagaaaaagt aattcaatca tttaaggtca tttattctat atataaatcc
     8041 aaatctgttt caggatattt taatttgaca agcgaaataa tcattttcag ttcttccatg
     8101 ttttctggat attctgggtc aaataccttg ctatgacggc caatcccgcc attgtcgatc
     8161 aatcggtaaa ttcgattcca ttcaatgaat tggtcaattg attggaaatt gctgattgga
     8221 tagccgccac aatccaaaat ctctgataca cgaaatgcta ggtatgcact atcgatcact
     8281 ttattttttt tcgtgtctat cttctcattt ctttcgataa gaattttttc aggacgatgt
     8341 ttcaagctcc actctaatag tagaaagaaa gcctccatcc gatactcttc ttcaaataac
     8401 cgaaaggctg aatcagaacg acgaaaaagc agcggaatca gataattttg tctataaatc
     8461 agcattggtg cgcggtccac catacactct aaataacggt tcagataccc tgtaccgaca
     8521 tagtttccta atcgatagcc ttcaacgccg aatcttgcgt atttttgcgc taattttgaa
     8581 caatcagtta cgttcacagc taactcttct actttgcggt aaccgcttaa gatacgttca
     8641 aaaggttcta aagtgatttt tgcttccata ttcttccccg tccatcttcc acatacttca
     8701 tatcatttca catgatactt atgctctcat cataccacag gttgaattag aaaagaaaat
     8761 aaatcacaca aaaaagcaag acaatctgtt agtgagattg tcttgctttt cgaaaaagac
     8821 tgaatacttt tcgttaaaac tggttgctta gtcttctaat gccacattat ggaacacatg
     8881 ctgtacatct tctagatctt ccagtgcatc aatcattttt tcaaatgttt cttgatcttc
     8941 tggagatagt gttacctcgt tttgagggat catttggatc tcagcgattg aaaaatcagt
     9001 gattcctttt tcttttaatg cttcttgtat actatggaaa tccgctactt caccgtaaac
     9061 gatgatttgt ccttcttctt ccattacatc gcgaacatcg atgtcttttt ccattaggta
     9121 ttcaaggatc tcgtcagcgt cttctcctgc aaaagcaaag attgctgtat tgtcaaacat
     9181 gtaagaaacc gctccggaaa cacccatgtt cccgccattt ttcccaaatg ctgcccgtac
     9241 gtctgctgct gttcgattca cattgttcgt caatgtatca acaatcacca ttgagccgct
     9301 tggtccaaat ccttcatagc gcaattcaga gtatgtttca tctcctgcac cttttgcttt
     9361 ttcgattgct cggtcaataa tatgttttgg tacattataa gttttagcgc gatcaatgac
     9421 gaaacgtagt ttttggtttg catgcggatc aggatcacct gattttgctg ccgcgtagat
     9481 ttcaatccca aattttgcat aaatccggct attattagca tctttagcag ttttctttgc
     9541 tacgatattt gcccatttac gtcccatatt ttcacttcgc tttctgattt gatagtttca
     9601 taacgcaaaa acgtcctgtt ccattatact tgtgaatcac gattctgtga agcttttttt
     9661 ctttcttcct tcattcttct catttttagt caattatcta tactcggatt gggatttgcc
     9721 cgaaattctg ttccggaata ttgcctccca attgatagat ttgatcactt ctaactaaca
     9781 gttccgcttc ttgtgcctct ttttttccga ttcgggaata aatagaaatc tctttttttt
     9841 cttttaagaa tcttcttcca gcatcggtaa atccaagtag tcgaagataa tttttatttt
     9901 gttggacagg aatctcttgc tttttgatat tcagcaagac ataacaagat aatcgttgga
     9961 tccgtgtcca tgtatatctt tttgttttta cctttgacac aaaatcctca aaactatcag
    10021 cagttgcagc tgctgttttc atgcgcgatt ctattccttc ggtcatctga tagatatctg
    10081 tcagttccag caaagtagaa gataacaacc gataacgcaa atacggccaa taagattccc
    10141 aagtctgcag cgaacaagtt gaaagatctt cttctgtctg aggcggcaca agtccagcta
    10201 ttctttttcc ttcttctaac gcatggcgaa tggctgttgc actggctatc ctctgatctg
    10261 tgatgatttt ttcatgatag cctgcacttt tacgtcggat cggatgtaat tccatagggt
    10321 ggacatactt ggcattttcc tttgcataac ttaatcccaa gatatgattt ggcgaagaaa
    10381 aatcaagcgt aatatcggga tacagactgc gaaaaacctc agtcattttt tgcggataac
    10441 tatacgtttg atcagacagt tcttgaaatg cctcttctat cctatcttga ttctcttgga
    10501 caaagcgtcc gaaactgctg taatcaaacg aagtctcttc ttctgttcca aaacacaaac
    10561 tgtcacagtc cagtgtttgc aagattttta ctgcaccttt agcaaaatag tcagctgact
    10621 gaaccgccca ttcgacggga agctccacta ctaaatccac accattttgt aaagcttcat
    10681 tcgcgcgatg ccatttatcg atgatagccg gttcgcctct ttgcaaaaaa ttcccgctca
    10741 ttacagcaat gacgacctct gctcctgtta tttttcgtgc catttccaca tgatagcgat
    10801 gtccattgtg aaaaggattg tactctacga taatcccgca gcttttcatg cgcatcctcc
    10861 ttcatagtta tcgataaaaa agaaatagag acaagtaaaa aaaggcaaaa atcggaaaaa
    10921 atacccgaac tatataaact attctcgcaa acacaatact tctgcgacga aaagctgcac
    10981 tgcaagagca atgcctatat cgttcggata tataatttat gttgaagatt tattcttttc
    11041 ttgttaactc gagcaggcaa aaccgctagt ctttgtatgc tacaaagaac cagcgctttg
    11101 atttctctga tgctgcttcg tctgtaaaat cagaaaatgc ttcgactttc gaaaagccgc
    11161 tgttttccag cattcttaaa taactttcca atggataggt acgctcttga tgtagttcat
    11221 cttcacgaat aaatcgacca gacccttctt gttcttcaac gaaaaaggta agaaaatgtt
    11281 cgatgctgtg tttctcgctt cctggataac tatcccacaa aaaagcaaaa ttatcccctt
    11341 gataatgata gctatattct ggaaaaacct catctacttg ataggtggag tgtacgtcaa
    11401 ataaaaatcg gccattttct tctaacgctt gatagacttc atcaaacact tgctgtacct
    11461 cttgtctatt tttcatatag cacagcgaat cagaaaaaca ggtaattgcc tgatattgtc
    11521 cgatttctga taaatcgagc atattgcctt ccacaaattg gatcgatgcg tcttcttcat
    11581 atgctcgctg gctggcaatc atcaacatct cttctgaaag gtctaaccct gtcactttat
    11641 atccatcttt tgcaaaggca accgctaatg ctccagtacc gcatgccagt tccaaaatat
    11701 tttttgtgtc ttcttgtaaa tgacgtttcg aaaaagctaa ccattgctga taaagtgttt
    11761 cgtccatcac ttcatcgtaa atgaaagcaa atgtttcata agccatatgt taatccaacc
    11821 attcactaag atcgactagc ggagcatctg accatagttt ttccagatta tagaaatttc
    11881 gttctggcgc atggaaaaca tgtacgatca catctccaag atcgatcaat acccattttc
    11941 cgccttcttt tccttcaata cgttttactt catatttgtt ttcttcttct ttatcaatga
    12001 tttcttctgt aattgcgttg atttgacgtt cgctatttgc tgaacagatc atgaaataat
    12061 ctgccaacaa ggaaacattt cgaacgtcta aagcgataat gtcttctgcc cgttttgaat
    12121 ctgctgcttc aatcgcaatt tttaattgtt ctttactatc tatgatgatt tcctcctaat
    12181 atgagcagct tatttttttg ctacccagta attatatgtt tcgattgttt ttggataaat
    12241 ctgttgttct tgcgcaagca aatgggccaa tgtatgtttt gtttcaaatg ctacagcttc
    12301 gtctagatca gcccatgcaa tggctcgtgc atcttgtacc ccaggaaaat ctcgacctgg
    12361 ttcaatatag tctgctacat aaatgatttt gtcaaggaga ctcatcttag cagccccagt
    12421 cgtatgcacc cggattgcat gaagaatctc tgcatcggtg attcctaatt cccgctctac
    12481 aaacgaagcg ccaacaagac catgccagat tgcattatta taattcaata actccggatc
    12541 aaaaccgtct cttacgatta ccattttgaa ctcctcatct ggtcgttctt ttgcataatc
    12601 atgtgtcaat gcagcaatac tagctttttc aggagaagca ccgtattttt tggcaagagc
    12661 cacggctgtt tcttctactc caagcacatg cttgaagcgt tgttcgctca ttctcatctg
    12721 tattttttgc atcaggtttt ctctagaata agcagtatat tcattactat agtccatatt
    12781 catacaaccc ttcattttga atataatcaa ttactttatc tggaacaagg tagcggatag
    12841 aacagccttc tttgatcttt tgacgtattt tcgtggaact gatgtcgatc tcaggtacat
    12901 ctacccaaat tacaggatat ggagtatccg ttgtgtatcc tggtcgacgt atacccacaa
    12961 agttgaccat agaagtcagc tcatcgattt tataccattt tggcaaatat tctaccatat
    13021 ccccaccgat aataaagtaa taatctgtat ccggattgtt ttgcgtcaat tccttcatag
    13081 tatcataggt gtaacttttc ccaccacgag ctaattcaat cgtttcaatc tgcaaaaaag
    13141 gattgtcttc caccgctaat tccaacatgt tgagtcgatg ttttgcatca atcgtctgtt
    13201 tctcatcaac atgaggtggc tgataagaag gcatcaaaaa tacgcggtca agacctaaat
    13261 tccggccagc ttgttcagcc atcactaaat gtgcaaggtg aacgggattg aatgttccgc
    13321 ccaagatccc tacttgcttt ttcttctcta agaattgagg catttcttca ggaaataatt
    13381 gtgaacgaac aaatgtctta gcttgcgtat tcatcatggt cactccttat attgctttta
    13441 cttcttttga tagcctttga tacttttctt ttgtggaagg tttgtataat acaataacac
    13501 gtccaatgat ttggacgatt tcgcaatcta tttctttttc taatacttga gctgcttctt
    13561 ctgcaatctc atctgtattt tgaagtaacg atactttgat cagttctctt ttttctaaag
    13621 cttcttcaat ttgaacgatc attgctgaat tgatcccacc ttttccgatt tgaaaaatcg
    13681 gctgaagatg atgcgctttg ctacgtaaaa aacgcttttg ttttcctctt aaactcataa
    13741 ttttccttct tccttaaatc aatgctttgc gtttcaatac atctacaccc tttggcgccc
    13801 agcccgcaat tactactggc gctgttacag tgatccagcc tagaccggca aaaacgatgt
    13861 ctgttttttc tttcacagaa aactcaaaac gaacaagctc cggaaatgcc tcgacttcat
    13921 caggacgagg aggctgtaat aatccaccta catgtttttg ataaaattcg tctgcttttt
    13981 ccagtttcgt tcgatgaata tttacatcat ttgacacata agcaataaat gaagcacgat
    14041 tgccgctgat aaaatcaaat cttgcaagtc cgcccaagaa aagtgtttgc ccttcattca
    14101 gctgataaac tttaggtttg atttctttta acggtgcaat gatccgcaaa tcttttttac
    14161 ctaagtagtg cgccatttga tggcgatgga taattcccgg tgtatcaatc aaaaaatgcc
    14221 cgtcatctaa aggaatctcg attttatcca aggtcgttcc tggaaactgc gaagttgtga
    14281 ttacatcttg tactcccgct gtatttttga tgatttgatt gataagcgtc gattttccga
    14341 catttgttac tccgacgata tagacatccc gtccatcgcg gtattcttcg attttttcta
    14401 atagttccgt catctcatga gatttcttag cactagttaa taatacatct actggacgaa
    14461 ttcctgcttc gtaagcacgt tccttcatcc attgtgttaa tttagttttt ttcaatgatt
    14521 taggtaatat atccactttg ttcccaacta aaagtactgg attgtcaccg acaaaacggt
    14581 gtagtcctgg gattagtgaa ccattgaagt caaaaatatc caccacattg acgatcaatg
    14641 catctttttg ccctaattca ttcagtaagc gcaaaaagtc gtcatccgtc aatgagacat
    14701 cttgggtctc attataatgg cgtaatctga aacaacgctg acaatagact tccccggaag
    14761 ctaaaccttt ttccaaagcc gtttgcggcg tgtatcctaa ttcaccgggt ttgtttgttt
    14821 gtataacggc accacagccg atacattgta attcttctgt cattcgattc cacctcgcca
    14881 gcccatatca gggtgttttg ctaatagata ggccataatt cttttttccc aaaaacgatt
    14941 aaattgtgtc ttccaactat ctgttgcgat aatgggtttt acaaggatgc tacgtatccc
    15001 tgcacgattg gctccacgta tatcagtcat aatctgatcg cctaccatta ctatctcatc
    15061 atctgctaaa tccaattgtt tctttgctat gttgatccct cttgctaaag gtttcattgc
    15121 tcgtgcaaca tacaaaagat caaatttttc aatcgctcgc gcgacacgtt ttgaattatt
    15181 gtttgaaaca acaacaacag ggattcctgc atttttcatt tctaaaatcc aagtaagcaa
    15241 ctcttctgtt ccatctggat tattccatgc aatcaacgta ttgtctaaat cggtcaatac
    15301 tgctttgatg cctagattct ttaattgggc aggtgtaatc tgatagatgg cttctaccat
    15361 ccatgttggt ttgtaatttg aaaacatgct gtctcctcat ctgcaaaaaa tgaaggagca
    15421 cataaagtac tcctcaaccc cgtattatac tgcatatcag caaaaaaaac tacctctata
    15481 ctcgttcgtc tttcagaaaa agcatttctt tacaaaatga ccaaaaataa agattgatca
    15541 cagaaaaatt tagaaacaaa aaacaactaa tattggcgga acctcttgta tcgtttttcc
    15601 agtaatttat gctgatctaa tcgaacaagt tccgtcaatc tttcaatcaa cgcttttttc
    15661 ataatgtgtg cgactttata tcgtggcaac tgctcgccgt tgaacgtttc aggaataact
    15721 cgatcaacga ttcctaactc gcgtagttct ggcgcagtaa ttttcatcaa ctcagctgct
    15781 tctgcggcgc gactaccgtc tttccacaaa atcgacgcaa atccttctgg agaaagcaca
    15841 gcataaatcg tttcttccag catccagact tcatccgcta aacctagagc aagggcaccg
    15901 ccacttccac cttcaccaat gatgatggcg atgatcggta cctttaaatc agacatttcg
    15961 agcagatttc tggcaatagc ctctccttcg ccgcgctctt ctgctcctac accgcaatat
    16021 gcacctgctg tattgatcaa ggtaatcacc ggtctattga acttttccgc ttgtttcatc
    16081 agtcgtaaag cttttcgata gccttcagga tggggagaac caaaatttct gcggatattt
    16141 tcctgcagat cccgtccttt ttgaataccg atcactgtta ccggtttgcc atctaatcga
    16201 gcgattccac caacaatcgc ttcgtcatct gcaaataaac gatcaccatg gcattcctcg
    16261 aaagaatcga agatctcttc aaaaaaatcc aagcttgtcc atcgttcttt agctcttgct
    16321 aactgaacga catcatgtgc tgtcttatcc acgatctatc catcctttct gtgtatgcaa
    16381 ttcaagaagc gtatggattt tctgtttcaa ttccatgcgg ggaacaatct gatcgacaaa
    16441 accatgagat aataagaatt cagccttttg gaaatcttct ggaagttcct gcttgatcgt
    16501 ctgttcgatg acgcgtcttc cagcaaaacc gatcaagctt tggggttctg ctagaataat
    16561 atcgccttcc attgcaaaac tggcagttac tcctccggtc gtcgggtccg ttaaaacagt
    16621 tagataaaaa agaccttcat tgctatgccg tttgacagca gcagagattt ttgccatctg
    16681 cattagagaa aagattcctt cttgcatccg tgcgccacct gaagctgtga acaagacgac
    16741 tggaagactt tcctttgttg ctcgttcgaa taatctggtg atcttttctc cgacaatcgt
    16801 tcccatactt cccatgataa aattaggatc catcactcca ataatgaagg gaatcccctg
    16861 aatggttgct ttaccagtga ggactgcttc atgaagtcct gttttatctt gcattttttt
    16921 aattttttcc gtatattcgg gaaatgctag cggatctttg gttacaagat ccgtatccca
    16981 ttcttcaaaa cttttttcat caataatcaa agctagacgc tgccatgcac cgattcgaaa
    17041 agcgtatccg caatgagggc atactttttc ttcaccgatg tctttcgtat aaaggatatg
    17101 tttgcaattt ggacactttg cccacatgtt atcaggaaca gatggcgagt tagcaggttt
    17161 gctctctcta ttaggattga tccgaatata tttctttttc ttaaacagag ccatctatgc
    17221 gcctcctatc tccgtttctg gtgtccaatt cggtaaaaat tcttcttgta aaaatgctgt
    17281 gctgtaatca cctgctatga ctgcaggatg actaatcaaa tccatctgaa attctgcgtt
    17341 cgtgatgatt ccttctgtga taaattcact taaggctcgc tgcatcttca ttaatgcttc
    17401 aaaacgcgta tttccatgga cgattatttt tgcaatcatt gaatcataat atggcgggat
    17461 cgtatatccc gggtaaactg cactgtctac tcgcagtccc atcccacctg ctggaagcag
    17521 taagttttgg atcttccctg gagaaggagc aaaatggaaa gcaggatttt ctgcattaat
    17581 ccggcattcg attgcgtgtc cttggaatac tacttcttct tgacacacat tcaaaggctc
    17641 accggctgct atctccacct gtttttttac taaatcgatt cctgtgacca tttctgttac
    17701 tggatgttcc acttggattc gagtattcat ttccatgaaa taaaaatcgc cttcttcatc
    17761 catcagaaac tcgatcgttc cggcattttc ataattgact gctttagctg ctcgaacagc
    17821 agcgtctcct agtgcttctc tttttgtttg tgagatcact acagaaggag attcttccag
    17881 tactttttga ttgttgcgtt gtaaagaaca atctcgctcg cctaaatgaa tgacatgacc
    17941 aaatcgatca cctaagatct gaacttctat atggcgagca ggataaatga ttttttccaa
    18001 atacatatca tcatttccaa atgccgctgc tgcttcttgc tgagctgaag aaaaatgctg
    18061 tgggagctct tcttttgatt gaactttccg aatgcctttc ccgccaccac ctgcagctgc
    18121 tttcagcatc actggataac caattctgtc tgctatttcc aatgcttctt ctactgtaga
    18181 taatacacca gtacttcctg gaatgactgg gacatttgct tcttgcatca attgacgcgc
    18241 attgatctta tttcccattg catcgatcgt tgcactttta ggaccaataa atgtgatatt
    18301 gcactcttca cacatggagg caaatcggct attttcagat aaaaaaccaa atcctggatg
    18361 aattgcttcg gcttttgtca cgattgcagc gctcagtact tcctgaacat tcagatatga
    18421 atcaacggct tttgccgggc caatgcagat tgcttcatct gctaactgtg tatgaagtgc
    18481 ttcggcatct gcttctgaat atatcgctac ggtttggatc cctagttcgc ggcatgcacg
    18541 aatgatacgt acagcgattt caccgcggtt agctatcaat actttagaaa acatatttca
    18601 cctatccaat cataaaggtt aattccgctt cggctacttt tttaccgtct actttagcga
    18661 ttcctttgcc tattccggct gaagctttca ctttcaatat ctcaacttca aggtataagg
    18721 tatctcccgg tgtaactttc ttacggaatt ttgctttgtc caatccgccg aaataagccg
    18781 tctttccctt gaattgttca agagaaagta atgcaactgc tcctgcttga gccattgctt
    18841 caacaatcaa tacccctggc ataacaggtt cttctggaaa atgtccttga aaaaagggct
    18901 cattgatcgt tacatttttc ttagcaacga tccgttctcc ttcaaccatt tcttccactc
    18961 gatcaatcaa aagcattgga taacgatgtg gaatgatttc ttttatttct tgtatgttca
    19021 ttgttttccc tctttcctgc tttgcttatt tttgctgaat cctaaagagt ggctgtccat
    19081 attcgacaac tgactcgttt tctacaagga tttctgtgat cactccgtcg gcctcactag
    19141 ttatctcatt catcagtttc atcgcttcaa taatgcagat aacttctcct ttggcaactt
    19201 gatcacctac ttttttgaaa gctggtttgt cgggcgctgg ttgcagataa acgattccca
    19261 cgattggcga aacaacctcg attccatcaa atactgccga ttctttttct ttcattattt
    19321 ctggagcagt tgttgcttgt tcaggtatga cattgactgg ctccgaaact ggcaacgttt
    19381 cgtttgtttg aaccatattc atgcctcgac ttgttttatt tttattcata tacaattcaa
    19441 attgaccttc acgcaaatcg aattccgtta gcgaggattg atcaaattga ctgactaatt
    19501 ctctgatttc attgatatcc aaaattattc ctcccagcgt ttcatgcaca gaaccgcatt
    19561 atgtccgcca aaccctaatg aattactcat tgcataagta aaacttgcag gtttgctttc
    19621 gtttttgaca actgtcaagt caatctcagg atcttgtttt tctacattga tcgtaggtgg
    19681 gataaattga tgttctaatg ccagcaaagt tgccaaggct tcgatcccgc cggctgctcc
    19741 caataaatgt ccagtcatcg atttcgtgct gctgacgtaa gtatctctga aattttcacc
    19801 taatgcgatt tggattgctt ttgcttctgc ctggtcattg gctggtgtac ttgttccgtg
    19861 agcattgata tatccgactt gttccggctg tacaccagct tcttccatcg caagcttcat
    19921 tgctttagat gcaccagaac catctggagt aggagctgtc atatggtaag cgtcacagtt
    19981 tgcaccgtat ccaacgattt ctccatagat tttggcaccg cgagcttgtg catgttctaa
    20041 agattcaaga actagaacgc ctgctccttc acccatcaca aaaccattcc gattttcgtc
    20101 aaacggaatc gaaccacgat taggatcagt tgattctgag agtgctgtta aagctgcaaa
    20161 tccagcaatc cccatctcac aaatcgttgc ttctgttcct ccagcaagaa tgacatcaga
    20221 atagccatgt ttgatgttac ggaatgcttc cccaattgaa ttcgttccgc tagcacaagc
    20281 tgttacgata gctgtacaaa taccttttgc acctacacgc aaagcaatat ttccagctgc
    20341 catgttggca atcgccattg gtacgaacat tggagaaact cgttttgcac ctttttcatg
    20401 tagacgaata acttggtttt cgattgttgg aagaccaccg atcccagatc cgatgatgac
    20461 gccaaaccgg tcttgattca ttttttcttt atctaaaccg ctcatctcca ttgcttctaa
    20521 agcagcgtag attccataga tcgaaaacat atccattcgt ttgctatctt ttttaataaa
    20581 atatttatcg taagggaaat ctttgacttc acccgctaag gtaatccctg tttctgaagc
    20641 atcaaattta gtgatcggtg cgatgccatt cgtcccctct tttaagctat tcaaaaagct
    20701 gtctgcgtca tttccaatag gtgaagtgac tccatagcca gtaattacca ctcgattcat
    20761 ttgtttgctc tccttttctt atccgtgcat gaccaatccg ccatcgacat tgatgacttg
    20821 tccggtgata tatggatttt gtgctaagaa aactgctgtt tgtgcaatat cttctacttg
    20881 tccgaaacgc tgcatcggaa tttgcttttc cgcttgttcc ttgactttgt ctgctaaaac
    20941 ttccgtcata tcagttgtga tgaatcctgg cgcaatggca ttacatgtaa tgcctctagt
    21001 tgcagcttct cgtgccacag attttgttaa tcctactacg ccagctttac tagctgcata
    21061 atttgcttga ccaatatttc caatcaaccc agatacacta gagagattaa tgatggcccc
    21121 ttctcgctgc ttcatcattt ttttcaatac gtgctgcgtc atattgaagg ttccaatcaa
    21181 attgatatcc agacattttt tgaaatcttc tgcatccatt cgcatcacaa gtttgtcatt
    21241 tgtgattccg gcattgttta ctaacacatg gatcgatcca agcttttctt ctgcttcttt
    21301 gatcatttgt cccgctttct cataatcaga gatgtcaccg gaaactccta cgcattttac
    21361 accgaatgct tcgatttctt ctatcttttc ttttggaatc tctccgcgac cattcaaaat
    21421 gatatttgct ccagcttttg caaaagctaa ggccatcgct ttgccgattc ctcttgtact
    21481 acctgtgata aatacatttt ttccttttac atccataacg ttcctcctta tagtattgct
    21541 attgtttcat caaatgtctg cttattctca acacgcagaa ctggtattgt tttatcgatt
    21601 tttttcataa atccgcttaa tacttttccg gggccgactt cgatgacttg ttcgatgccg
    21661 attgttttta acttatggat actctcatag aaacgaacag gttgcatgac ttgctgttca
    21721 agtagtcccg cgattgtttc tttcttcatt atttccgttg ttgtgttgct tatgatagga
    21781 aaagaaggtt cagagaattg aatctgactt aggtccttcg ccagtttttt tgctgccggt
    21841 tctaaaatag ctgtatgaaa aggcccgctg acatttagag ggatcattct tttgaaccct
    21901 ttttctttta atagtaaaac agcttcgtct actgcttttt cttcgccacc aatcacgatc
    21961 tgctgaggtg tattataatt tgcaggagat acgatgccgt acttgctagc ttcatgacag
    22021 ctttcctcga tcgtttcaat cggcgcattc atcactgcaa ccattttccc gcttccagct
    22081 ggggcagctt ctgtcatata agcgcctctt tttgctacta acgcaactgc ttcagtgaag
    22141 gatagcgcat tactagctac taatgccgaa tattctccta aactcagtcc taaagctgca
    22201 tctgggatga ttccatgctc cttcaataag cgataatacg cgatgctgac tgttagaata
    22261 gctggctgtg tatattgcgt ctgatccaaa cgttcatttt ctgtaaagca aagttcagcc
    22321 atatcgtagc cgagaatctc acttgcttca tcaaatgtct gctttacaac ggcttcttca
    22381 tataaatctt ttcccatacc ttgatattgt gcaccttggc cactaaataa gaatgctgtt
    22441 ttcatgtttt tctccaatat atccagaata tatgtttgat tcttcctatt caaaaaggcc
    22501 gagacattcc tatttcagga aatgtcccag cccccaacct aattttaatg attatgcagt
    22561 ttgtttttct acgtaagtca ctaaatcttg aaccgttttg attccgtctt ctgtttcgat
    22621 tttcacgtca aattcatctt caatttcatt gatgatttgg aataaatcca agctgtcagc
    22681 ttctaagtct tcttggatgt cagtagtcaa tttgatttct tcagtttctt ttcctaattc
    22741 ttctgcgatg atttcttgaa ttttttcgaa taccataata atatattcct ccgatattgt
    22801 ttgcttttta tttttataat ttaacagtct acaactccat gagaatactt ccccatgtta
    22861 aacctccgcc atagcctgta aatattactt tctgctgttt tcctaaggtg atctttccac
    22921 tagaaactgc ttcatccaat aagataggga tgcttgcagc agatgtattc ccatatttat
    22981 ccatattggt caggaacttt tcttgcggca tctttacttt tcgggcaatt ttctcaataa
    23041 ttctgaggtt tgcctgatgc aaaagcagat aatcaactgg tttgtctttc atgacttctt
    23101 ggatattgtt tgcaacgtct cgtacggcaa aatcaaaaat atctctgcca gtcatttgca
    23161 aataatacga accttctgaa tcttctgaat agaacggatt tttattttgc tggtagccag
    23221 cggtcaatga gctacttctg gtaccgtcag ctgctaattt ttctttcagc aattttttct
    23281 gtggattcgc ttctaagaga atcccacctg ctccatcgcc aaacaacaca gcagtcgtac
    23341 gatcagacca atcgattagc ttagatagtg tttcaccacc aatcaccagt cccttagttg
    23401 agccgcagcg gatcattttt tcagccatgc ttaacgcata aacaaatcct gagcacgcag
    23461 cactgatatc aaaagcgata gcatgagttg ctcccaattt cccttgtact tgtgctgcta
    23521 cagatggtgt cgtgtaatcg ggcgacattg ttgctactaa aataaaatcc aaatctttag
    23581 ggtttagatt tttctgtttt atcaattttt ccgccactaa aatgcataaa tcagaagtct
    23641 cctgatttgt tacacatctt cgttcacgaa tacccgtcct tgacgaaatc cattcatcag
    23701 aggtatccat catcttggaa agatcattat tagtaataat cttttcagga aggacgctgg
    23761 ctgttgcagt aatcattcca aattgttcca ttaaatatcc tcacttatag tcttgcaaga
    23821 agtcgtgcaa gtttttcagt gcatccagca aggcatgctg ttcctccgta gccattccgt
    23881 ctaagatgtt cttgaccatt tcacgatgaa aatgctgatg gacccgaaat agtaactttc
    23941 cttttttcgt tagtcccaac tttacaacgc ggcggtcatc ttcactgcgg atacgttcga
    24001 catagccttt ttttactaac ttgttaatag cagtggtcaa ggtcccaaca gtaattgata
    24061 gttctttggc tacttctgaa gttgtttttt tcttgtacat cccgattgcc tcgatcgtgt
    24121 gcatctctgt gattgacaga tctttgaatt gagatttttt cagctcagac tcttcgattg
    24181 tcaaaatatc attgaagaca ctaacaagat aatcatttac tgtttttaaa tctggttcca
    24241 tgttccaccg tccactaatt agtttgatta tcaaatcttt tgatgttcaa actatatctt
    24301 aacttttttc aaaatgcaac cttaaaagat cattttttta ctatat
//
LOCUS       DACCXW010000032        24259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCXW010000032 DACCXW010000000
VERSION     DACCXW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.032"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="IVY71_001062"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="IVY71_001062"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7985129.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2485)
                     /gene="purK"
                     /locus_tag="IVY71_001063"
     CDS             complement(1352..2485)
                     /gene="purK"
                     /locus_tag="IVY71_001063"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7985130.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="IVY71_001064"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="IVY71_001064"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7985131.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="IVY71_001065"
     CDS             complement(3373..4680)
                     /locus_tag="IVY71_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7985132.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="IVY71_001066"
     CDS             complement(4683..5264)
                     /locus_tag="IVY71_001066"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7985133.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="IVY71_001067"
     CDS             complement(5836..7128)
                     /locus_tag="IVY71_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7985134.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="IVY71_001068"
     CDS             complement(7125..7748)
                     /locus_tag="IVY71_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985135.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="IVY71_001069"
     CDS             complement(7751..8587)
                     /locus_tag="IVY71_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7985136.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="IVY71_001070"
     CDS             complement(8604..9491)
                     /locus_tag="IVY71_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7985137.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="IVY71_001071"
     CDS             complement(9507..10790)
                     /locus_tag="IVY71_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7985138.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="IVY71_001072"
     CDS             complement(11473..12156)
                     /locus_tag="IVY71_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7985139.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="IVY71_001073"
     CDS             complement(12174..12791)
                     /locus_tag="IVY71_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7985140.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="IVY71_001074"
     CDS             complement(12801..13625)
                     /locus_tag="IVY71_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7985141.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="IVY71_001075"
     CDS             complement(13618..14178)
                     /locus_tag="IVY71_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985142.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="IVY71_001076"
     CDS             14352..15308
                     /locus_tag="IVY71_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7985143.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="IVY71_001077"
     CDS             complement(15613..16569)
                     /locus_tag="IVY71_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7985144.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="IVY71_001078"
     CDS             16673..17275
                     /locus_tag="IVY71_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7985145.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="IVY71_001079"
     CDS             complement(17363..18862)
                     /locus_tag="IVY71_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7985146.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="IVY71_001080"
     CDS             complement(18878..19402)
                     /locus_tag="IVY71_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985147.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="IVY71_001081"
     CDS             19700..20449
                     /locus_tag="IVY71_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7985148.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="IVY71_001082"
     CDS             20440..21225
                     /locus_tag="IVY71_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7985149.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="IVY71_001083"
     CDS             21194..21910
                     /locus_tag="IVY71_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7985150.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="IVY71_001084"
     CDS             complement(22012..23031)
                     /locus_tag="IVY71_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7985151.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24108)
                     /locus_tag="IVY71_001085"
     CDS             complement(23050..24108)
                     /locus_tag="IVY71_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7985152.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7624 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgatttt
//
LOCUS       DACCXW010000033        23945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCXW010000033 DACCXW010000000
VERSION     DACCXW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.033"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            14..646
                     /locus_tag="IVY71_001086"
     CDS             14..646
                     /locus_tag="IVY71_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985153.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(780..905)
                     /locus_tag="IVY71_001087"
     CDS             complement(780..905)
                     /locus_tag="IVY71_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7985154.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1039..1932)
                     /locus_tag="IVY71_001088"
     CDS             complement(1039..1932)
                     /locus_tag="IVY71_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7985155.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2114..3040)
                     /locus_tag="IVY71_001089"
     CDS             complement(2114..3040)
                     /locus_tag="IVY71_001089"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7985156.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="IVY71_001090"
     CDS             complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="IVY71_001090"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7985157.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="IVY71_001091"
     CDS             complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="IVY71_001091"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7985158.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6638..9088)
                     /gene="parC"
                     /locus_tag="IVY71_001092"
     CDS             complement(6638..9088)
                     /gene="parC"
                     /locus_tag="IVY71_001092"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7985159.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9100..11145)
                     /gene="parE"
                     /locus_tag="IVY71_001093"
     CDS             complement(9100..11145)
                     /gene="parE"
                     /locus_tag="IVY71_001093"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7985160.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11311..11745)
                     /locus_tag="IVY71_001094"
     CDS             complement(11311..11745)
                     /locus_tag="IVY71_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7985161.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11872..12510
                     /gene="plsY"
                     /locus_tag="IVY71_001095"
     CDS             11872..12510
                     /gene="plsY"
                     /locus_tag="IVY71_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7985162.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12642..13517)
                     /locus_tag="IVY71_001096"
     CDS             complement(12642..13517)
                     /locus_tag="IVY71_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7985163.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13599..14390)
                     /gene="codY"
                     /locus_tag="IVY71_001097"
     CDS             complement(13599..14390)
                     /gene="codY"
                     /locus_tag="IVY71_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7985164.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="IVY71_001098"
     CDS             complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="IVY71_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7985165.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="IVY71_001099"
     CDS             complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="IVY71_001099"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7985166.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="IVY71_001100"
     CDS             complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="IVY71_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7985167.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="IVY71_001101"
     CDS             complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="IVY71_001101"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7985168.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18855..20933)
                     /gene="topA"
                     /locus_tag="IVY71_001102"
     CDS             complement(18855..20933)
                     /gene="topA"
                     /locus_tag="IVY71_001102"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7985169.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="IVY71_001103"
     CDS             complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="IVY71_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7985170.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21968..22735)
                     /locus_tag="IVY71_001104"
     CDS             complement(21968..22735)
                     /locus_tag="IVY71_001104"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7985171.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="IVY71_001105"
     CDS             complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="IVY71_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7985172.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6247 a         5147 c         4117 g         8434 t
ORIGIN      
        1 atttggagat attatggaaa ttcaacgtag aaccactaat tttttacatt taggctcttt
       61 ttttgcaact ttgacgatct tattttttct atgttattca taccttaaga cctcacatgc
      121 gccttctagt gcattaggct ggttgctttt ggatcattct gatatcacag aagccgaaga
      181 aaatccgttt tttttgattt tgtcttctct ttgcggctta ctgttttctg tttatttcgg
      241 tgcctatttt tttcatcgac atcctggtac gttaaacgaa gcttcaagcg tattgattgc
      301 atttaaaacg ttttcttttt tagcaattct tttttattcg tttaatcaca atacgctttt
      361 cttctttatc atgaatggtt tagtcgtgat tttatccatt tttacttatt ttattttttc
      421 actcattttt agccaattga aatgtccgct cttctttcga ttagttcctg tcacctgttt
      481 tatttacagc ttgttttcct tttttgtcct tgctttaatg ccactgacca acccttcgac
      541 gatccaagtc gcaggaaatc tgttggatat gctcttcatc gtcagtctag gtgtatttat
      601 gtggcaaaga aaaaacaaac aaaataaaaa gcattttgaa gcctgactat tacgacttca
      661 taaatgcttt tttagtttta aacacttcaa tctaaaagaa taacccaaga ttggataaat
      721 tattaatgac tcgccattcg tttcttagtg attctggatt atcaaatgga cttcctaatc
      781 taatcagcta gttcttccca taaaatgcca tacttgactg aatcataata ttgattattt
      841 aaaaatctaa cttttcttat cactacttcc tttgtcattc cacatttctc accatttttt
      901 tgcattcctt tgtttccaga ccatgtagtg aatctaatag gtggtagata ggcaaaatac
      961 tcaaataatt cttttaacca taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa
     1021 tttttgctta ctgctctctt aggttttact cactaaaact gttccatatt ctttgatata
     1081 agaaaatgca tgcggtataa atttcttgct cagctcagaa aagatttgtc gtttaagaaa
     1141 ggccatttcc tccattgtca atgaaggatc caatgttggt aattcaatct caaaattgtt
     1201 tttttcgaca ataggtaatt ctaatgtgaa tgattcttct tcctttatag tattcgtcac
     1261 aagtagagac tgatggaaaa tactaggccg gcgattttta tatttattct tacgtatcct
     1321 gtcaacgata acttctattt gatcaaaaaa gtacgattca tttacgtctc caataagttc
     1381 ggctaattct ttgaatgcct tcgttacagg aagctgattc ttctgtacaa aaaagtaatt
     1441 agccaaagta agcgggaagt gttctataga ggaaagtgtg ataaaacggt acttctcatt
     1501 tcctgcagtt gtcctcagca attctgtctc tcggctgttt cttactccat aaaaatacgc
     1561 ttctttccgt tcatcttcga aacaatacgc ccaaataact tctcccatcg ctaatttttg
     1621 ttcatcaaca gagagtctct tcaattgttc agctatggtt tcagcagccg aagtggcttg
     1681 ttgcaagtaa tctttaggat caaatattgg aaagttcaaa tgatatcttc gttcttccct
     1741 cttgattaat cctgcatcga tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa
     1801 ttcttttttc aattctctta gaatcgtttc ttgttctcga tgttcatata gataatcggt
     1861 caatgaagaa aaaagcggat catcggtcag tttttgataa aagttttcct tattttttga
     1921 ataatagttc atatatgttc tcttttctat ccgttttatt ttacttaaaa ggcttttacg
     1981 taaaaaggag gaagaacaag agtcataatt gactgttatt ctcctccatt tcttcggcct
     2041 ttagtatgtt ctatgtttct ttttatatca atcgcttatc cattcatttt gaatgcttca
     2101 tactaccatt tgattagcta aatacagccg tcaattgagg tacgacttgt tttttacgtg
     2161 aaacgacacc tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta
     2221 ctttttcttt tcctttacca gcaacaagta attctgaatc actgtctaag atgtttgtga
     2281 caactaatac gaacagatcg tagccatttg ctttcatttc atcttccatt gctgcttcta
     2341 gctctgcttg tcgatccatc acttcattaa ggtccactgt attgacttga gcgatacgaa
     2401 cgttggaact gcccattggg aaactttttg catccaaatc aagaagaacc gcagctgatt
     2461 tatcacttag atttgttcct gctttcaaca tttcaagtcc ataagtgttt agatcgactt
     2521 cagcgattgc tgcaagttct tttgcagctg cttcatcttc aggcgtacat gttggtgatt
     2581 tgaacaacaa tgtatctgaa atgatagctg agagcatgat tcctgcgatt tctttaggaa
     2641 ttgttacttg gttttcttta tatagtttta aaataatcgt acttgtacat ccaactggtt
     2701 ctgcacgata gtacaatgga ttagctgttt caaagtttgc aatacgatgg tggtctacta
     2761 cagctaaaat atttacttcg ctgatatctg aaatactttg ttggaattca ttatgatcca
     2821 caagcatgac atctgcattt tctgctttag ctgactctac tactcgtggt gcagatagct
     2881 gaaagtggtc taatgcgtat tgtgtttctt cattaggtgt acctagagca actgcttctg
     2941 tttctttacc taattcattt tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg
     3001 tatctggatt ttggtgacca aaaactaaaa tttttgacat gtcattcgct cctttgtgat
     3061 ttctcctatt taataatagc aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg
     3121 cgacctatcg cgttaaatat tttgtatact cttctgtatg aagcaatcgt ttagcatttt
     3181 cgacacgttc ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt
     3241 cccacttgta ttttcccatc gtatgataag gaagaatctc tactctgtct acatttgaca
     3301 aggtttgaat aaagttgtct agtcggatca aatattcatc ataatcactt cgctcaggaa
     3361 ctagtacatg gcgaatccaa accggtttgt tgatttttga caaatattga gccatttcta
     3421 aaatattttc attgtcccaa tgcgttagtt ttttatgctc ttcattatcg atgtgtttga
     3481 tgtcaaataa cagtaaatct gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt
     3541 cttcatacgt aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg
     3601 cttttttaaa tagttcgatc aaaaaatcga tctgcaatag aggttctcca ccactaacag
     3661 taataccacc tttatctccc cagtaaggtc tgaagcgttc tgctttatct aaaagttcat
     3721 ctgctgtgta ttctttccct ccgccaatat tccaagtatc tggattgtga caaaattggc
     3781 aacgcatgcg gcatccttgc ataaacacga caaagcgcag tccaggtccg tctactgaac
     3841 cgaaagtttc gatagaatga acatagccga ttgttttttc ttccatttaa gttcacatct
     3901 tttctttgat ttcattttaa gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat
     3961 ttttcgcaat tgccgcttcg ttatgtgtaa aatttacagg gacggaacat acaagtcctg
     4021 cccctgtaaa tcgtttcttt caaataagca tactgattat tcgattttga tgcgatggct
     4081 attcaacgtc ctttcgacgc taagtcgaaa aacttttttc acgaactttt tacattctgt
     4141 cgtgtgaagt tctcatcaag acatctaatt gttgttctct tgtcaagtca cggaatttaa
     4201 ctgcataacc agatacccgg atcgtcaagt tcggatattt cattggattt tccattgcat
     4261 cacgcaataa atcattactg aatacattga cattcaaatg ataagctcct tttgagaagt
     4321 atccatctaa gacattacgc aagttttcga ttcttgtatc ttgttctttt ccaagacctg
     4381 aaggattgat tgtttgggta tttgaaattc catccaatgc acagccgtaa ttcattttag
     4441 ctgttgagtt caggctagct aataacccac tttgttccgc attgtaactt ggatttgctc
     4501 ctggcgcaag tggttttcca gcttcacggc catcaggagt atttcctgta tttttaccgt
     4561 aaacaacatt tgaagtaatc gtcaataatg aagttgttgg ttttgagttg cggtaagttt
     4621 tatgacgttt gatttgtccc atgaagtatt ccaagatcca tgaaactaat tcatctgcac
     4681 ggtcatcatc atttccgtat ttagggaagt cgccatccac ttcaaaatca actgctattc
     4741 ctttttcatt acggattgct tttacttttg catatttgat cgctgacaag ctgtctgccg
     4801 catgagagat cccagcaata cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag
     4861 ccagctgagc tgcttcataa gaatatttat catgcatata atgaatgatg ttcaacgtat
     4921 tgacatatag ttctgctaac caatccatca tattcatgaa gttatgtttc acttcttcaa
     4981 agtctagtat ttctgatgtg attggacgat attctggtcc aacttgggcg ccagtcactt
     5041 catcgactcc gccattgatg gcatataata gtgttttagc caagttagca cgagcgccaa
     5101 agaattgcat gtcttttccg acaactgttc ctgatacaca gcaagcaatc gcacaatcat
     5161 cagaacccca ttgttcacga agctgattat cattttcgaa ttgaatcgaa gaactttcga
     5221 tagcgattct gctagcatat gttctgaatc cttctggaag gtgttcagaa tacaaaacag
     5281 tcaagtttgg ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga cggaaatcat
     5341 tctttgtaac taatgaacga ccatctaatc ccatacctgc aatggataaa gtagcccaga
     5401 tagggaatcc tgagaataat tggttatatt caggtgtacg cgcgaatttg accatacgta
     5461 atttcattac taagtggtcg atcaattctt gtgcttcttt ttctgtgatt actccattat
     5521 ccaaatcacg ttggatatag atatcaagga atgctgagat acgtccgatt gacatcgcag
     5581 caccattttg tgatttgatt gctgctaaat atccgaagta tagccattgg acagcttctt
     5641 ttgcatttgc agctggttta gaaatatcat agccatattt cgcagccatt tctttcattt
     5701 gcgctaatgc acgatattgt tcagtgattt cttcacgtaa acgaatcact tcttcactca
     5761 ttgtatgata accagtcata tcatgatctt ttttcttttg ttccatcaaa tagtcgattc
     5821 catacaatgc cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa
     5881 gccctgtaat gattttgttt ttacgagcag cacgcatttc agcagtataa gcgtcaaata
     5941 caccttgatt atgtgttttg cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg
     6001 tatagccatt gctttttagc gcattgttag ccatattgat tccgccgaac ggcatgaatg
     6061 cttgtttcaa aggtttgtct gtctgtagac cgacgatttt ttccaaatct ttatccaaat
     6121 agcctgcatc gtgtgaagta atagttgata cgatttctgt gtccatatca tagacaccgt
     6181 tacgctcgaa ttggatatca tttaactttt gcaattcatc ccataatttt tctgttgctt
     6241 ctgttgggcc ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta ttttgaataa
     6301 agtctctagt gtctacgcct tctttccatt tggtaccttt aaatccattc cattgctcca
     6361 ttgtatggac ctcctaaatt tctgttttat gtaacagttt ttacgtgatt agtataacac
     6421 agcaccctat ctttgcaagg gttttcttgt tttttattta acaattttta tgttttattt
     6481 acatatattt aaatcaacgt tgacatacca acatttaagc attgtgaaca ctttcatctt
     6541 tttttgcaaa cgtataagga tttattaaaa atgaatgaaa aagagacgcc acctgtatta
     6601 atacagatgg cgtctcttca ttaaaatact acaacagtta cgactcgatg aaaatctcag
     6661 gagcatcgat aacttcgatg acttctccac cttttttctc atccatcgca aatgatccat
     6721 tcgatgttcg atcacctata gggaaatcag ttgggtcaat cgttaactgc tgcccttttt
     6781 gattcatcaa ggttatttct tttgatcttc cttccgacat atagataata cggtgcggat
     6841 tttttttcag ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt tggctgattt
     6901 cttgagcaag catgcgttta acagctcctc gctgagtaac gattacgatc ggcgtatcac
     6961 cttctgctaa cacaagcagc ccgttgataa tcctatctcc atctttcaaa ttcatagact
     7021 tcactccagc tgcctttgct ccaacaacgg gaacatctgt taaaggatag cgcaaaccca
     7081 ttccttgatg cgttactaag aaaacatcta agcctggctg atcttctttc agatacacag
     7141 caatcagttc atcttcttcc gcctttagct tcatacagct gagcggacgg ctcttatagg
     7201 tgcgccatgg ttcgaactct gtcattcgtg tttgcttgat caatccattc ttgctgatga
     7261 atacaaatgt tttctctgct tgaatttttt gatatgggaa aacagctaaa atcgattcgt
     7321 ctacagacag attggagatt gtttgagaaa tgtgctcacc agcatctttc catttcaaat
     7381 cgggtagttc atggacaggg cggtagatca catttgcttt atttgtgatc agaagaacat
     7441 ggtccaatgt actcaattca cctgagtata gcaagtagtc gccttctcgc atcccgattt
     7501 cttctggttt ggaagcacta taagaacgaa ggcttgttcg cttcacatag ccttctcgcg
     7561 tcacactgac aattacgtct tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt
     7621 cgatttcgtc ttcgattgtc gttagacgag cggaagaata attttttttc acttctcgca
     7681 attctttttt cataacagag aaaagctctt tgtcatttga aaggatttta ttcaactctg
     7741 tgatttgcgc aatcaagctt tcggattcct tttgaagctc tgtgatatcc gtgttcgtca
     7801 aacggtataa ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt
     7861 cgactaaatt ctttttggcg tcttttttgt ctttactttc acgaatcgtt gcgattactt
     7921 catccagaat cgataaagct ttcatcaatc cttctacgat atgctgtctt ttttgagctt
     7981 tggctaaatc gaattgacta cgttttgtta tgacttgctt tcgatgctcg atataacttc
     8041 ttaaaatgtc tttcagtcca acttgatgcg gagtcatatg gtcgatagcg accatattga
     8101 aattgtaatt gatctgtagc tcagtgtttt tgaacaaata attcaaaatt ccttgagcat
     8161 tggcgtcttt tttcaattcg acgacaattt gcagacctgt tctatcactt tcatctcgta
     8221 cttcagctat tccatcgatt ttcttattca aacggatctc atccattttt ttcaccaagg
     8281 tcgctttatt tacttcatat gggatttctg tgatcacgat ttgctgttta ttacccttca
     8341 ttgcctctat ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt tcataagctt
     8401 ttttgatttc atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat
     8461 attccatcaa tttttcaagg ctcgcttgtg gatgatcgat catatagatt gtgccatcga
     8521 tcacttcagc taagttatga gtcgggattt ctgttgcata tccagcagat atacctgtcg
     8581 atccattgac gagaaggttt gggtatcgag ctggaaggac tgttggttct ttttctgtat
     8641 catcaaaatt ccacacgaga tcgactgttt ctttctcgat atctgctagc atctctccac
     8701 ttaacttgga taatctggct tcagtataac gcatcgcagc aggtggatca ccgtccatac
     8761 tcccgttatt cccgtgcatt tcgatcagta cttcccgcaa cttccagtct tggctcatgc
     8821 ggaccatcgc ttcataaata ctgatgtcac catgaggatg ataattcccc atgatatttc
     8881 cgacagattt tgctgattta cggaatcctt tgtcatacgt attgccatct ttgttcatgg
     8941 caaataatat ccggcgttgc actggcttca atccatcacg gatatcaggc agtgcacgtt
     9001 cttggataat atatttggaa taacgtccga agcggtcacc catcacttct tctaaagtta
     9061 attcttgtac ttcttgtcga ttttccatca ggtgacctcc tattctactt caaataactg
     9121 attgttttca tttttatctg ctcgttcttc atctaataaa tcattagaaa ctgaaggaga
     9181 gatttcagta tcctcttttt tatctaagat actgccatct tcttcgagcg taaattgcac
     9241 gtgattttcg atccattttc ttcgaggttc tactttgtca cccatcagtg tggttactcg
     9301 acgttcagct tgcgcagcat catcgatacg gacgcggatc aacgtacggg aagtcggatc
     9361 catcgttgtt tcccacaatt gttccgcatt catctctcca agtcctttat atcgctgcag
     9421 catatatcct ttgccgactt ttttaatcat tgctgccaat tcgtcatctg tccacgcata
     9481 ttcaatgact tgcttctttc cttgtccttt ggatacttta tataaaggcg gtaacgcgat
     9541 atatactttc ccagcttcaa tcagcggttt catataacga taaaagaaag taagcagtaa
     9601 tacttgaata tgggcgccgt ctgtatccgc atcggtcatg atgattactt tgtcgtaatt
     9661 acagtcttcg atagaaaact caggaccgac tcctgcacca atcgtataga tcatcgtatt
     9721 gatttcttca tttttcaaga tatcttgcat tttagctttt tcggtattga tgacttttcc
     9781 tcgtaaaggt agaattgctt ggaactttct gtctcttcct tgtttagcag atccgccagc
     9841 agaatcccct tcgactaaat acaattcatt tttcttaggg tttcgggatt gtgcgggtgt
     9901 caattttcct gaaagaagag attcgccttt tttacgcttc ttcccatttc ggctttcttc
     9961 tctggcttta cgtgctgctt ctctcgcttc tctggctttg attgctttac ggaccaacat
    10021 ctggctcatt tcgctatttt cctgcaaata aaagcccatt tgttcactga tgacgttatc
    10081 tactacggta cgagcaactg gcgtaccaag tttttctttt gtttgccctt caaattgcag
    10141 aagattttca ggtacacgga tagaaagtac agcagctaac ccttctctga aatcactacc
    10201 ttccagattc ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt cattataaga
    10261 tttcgtcatc gccgctttca taccagcttc atgggttccc ccatcttttg tccgaacgtt
    10321 attgacaaaa gataaaacat tttctgaata accgtcattg tattgataag cgacctctac
    10381 ttcgatacct tctttttctc ctgaaaaata aacaactggt gtcaatgtat ctttttcttc
    10441 attcagataa tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg
    10501 ttcttctcca cgaagatcag aaagagagat tttaacgcct tttagaagaa aggcagattc
    10561 tcttaaccgt tcggctaaaa tttcatatga aaatttcgtt gtactgaata ttgtatcatc
    10621 tggtaaaaaa tggacagtcg tcccgtttgc tttttttgtt tttcctatct ttttcaaagt
    10681 accgtcaggt ttaccgccgt ttttaaattt ctgctgatac tctacgccat ctctaacgat
    10741 cgttacaatt agccatttcg ataaggcatt taccacactg gctcctacac cgtgcagtcc
    10801 accagaagtt ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat
    10861 cacttcaact gttggtattc cggaagcatg cattcctaca ggcatcccac gtccgctatc
    10921 tgcaacagtt atactattgt cttcatggat ggtcacatca atctcactgc cataacctga
    10981 taatgcttcg tcaactgcat tatcgacgat ttcatagact aaatgatgca agcctcggct
    11041 gtcagtggat ccaatgtaca tccctggtct ttttctaact gcctctaatc cttccaatac
    11101 ttggatggac gagtcattgt attcgttgtt tacttttttt gccaaggcaa acgctccttc
    11161 aataatactt tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa
    11221 aagagggtag acgagaaatc aattttaatt atctcctaac cgctttttcg acattcccct
    11281 ctatcgatca tctcttcttt gtggtcattt ttatttttgg ttttctttca acttaccaag
    11341 ttcaatctta atacaacgat ccattacgat atctgtacgt cctgcttttc ttagcatctc
    11401 agctgcttct ttgttttcaa tccctaattg tgcccagaac actttagcat cagttttcaa
    11461 aaaatcacct gctatttcag gtaaaaattc actccgtcta aatacatcta cgatatcgat
    11521 tggatcatcg atttctgtca aattcgcata gactttctcg cctagaattt cttcaccagc
    11581 caataatgga tttactggta tgattttata tccattgtct tgcaatacct gtgcaaccat
    11641 gtaacttgtt cgttctggtt tattgctcaa gcctacgacg gcaatatttt ttgcttcttc
    11701 caaatagttg aaaatcgttt cttgtgttgg attttttaca gacatttctg ccaccccttg
    11761 cttttctttt tattccatct attatacttg tatctttcat tttttcaatt gttcctgtta
    11821 cctattcatg ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa aatgaaattg
    11881 atcttattac ttattttagc atatctttta ggttctattc cttccggtgt ctggattggc
    11941 aaattgtttt tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg aacgaccaat
    12001 acatttcgtg tattaggcaa aacagccggg attgttgtcc tgcttatgga tattttaaaa
    12061 ggtacattgg ctacttgttt gccgatgatc ttccaccttt ctgggattaa ccctctttgg
    12121 tttggggtat gcgccattct tggccataca tttccgattt ttgctgggtt caaaggagga
    12181 aaagctgttg cgacaagtgc aggaatgctt ttaggattta atcctgtatt ttttgtttat
    12241 tcttgtatca tctttatcgt tagtttattt tgtacaagta tggtgagttt gagcagtatg
    12301 atcagtgcag tattgatcac tttatctacg atcgtccttc cttatgtagc tcctgtgatt
    12361 ttggctaagc ccaattggtt actgacgatc attgcctttt tagtttcttc ctttatcttt
    12421 tatcggcata aagacaatat caagcgtatc agaaaccata ctgaaagtcg aatcccgttt
    12481 ggtttgaata gcagcaagaa aaaatcctga tactttttca ttccaaagag ataaaacggg
    12541 agtgtgacaa atcgatgtca cactccctta ttacttacag gatagctttt ttacacacct
    12601 tggtaaacgg tgttccctgc taacttctcg gtcacaacct cttacttcac tgtgatggaa
    12661 tattttgtct tgaagatttc atgggcacct agttgattga tccctacttt ttccatcaag
    12721 tttccactac tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc
    12781 gcttcttttg gataaggaga ccagatccct acataaggca tatctttata gcttaaagtt
    12841 acgctatggg aagatttttc acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa
    12901 accaaggcat cttgctcaaa aagttcacga ttcaaagcca gattcgtatt tgtctgacct
    12961 aacgtctttt gatccatatc gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc
    13021 aatggagaaa atgccagata gaaatcttca aatgacaatc cttcttcaag aggtacattg
    13081 aaagcaggat gtccaccgat agaaaagaac atttcttttt caccggtatt tgccacttga
    13141 taatgaaccg tgatgccgtc tcctcctaat tcatagctga tcactaattc aaaatcaaat
    13201 ggatactttt ctaatgtctc cggcgtactc cttaagatga aactagccat ttcttgtcca
    13261 tgttcaatga cttcaaaatc catatcccga gcaaatccgt gttgccccat ttgatatgtt
    13321 ttcccttgat aaacatactg atcatctttt aaacgtccaa caaaagggaa taaaaccggt
    13381 gcatgtctgc cccaatacgc tggatcacct tgccagatat actctaaacc atgttctttt
    13441 gatttcagac tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt
    13501 tgaataataa cagtcattgc cattcctcca acttcgactt actttttaaa atgggaaggc
    13561 tggaacaaac atttctatct gccccagtcc ccttacttct aataactgtg ttttgctaac
    13621 tcgtctttaa aacgggaatt caatactttt aaataagttc ccttcatgcc taacgaacga
    13681 gattcaatga ttccagctga ttccaatttt cgtaaagcat tgacgatcac agaacgagtg
    13741 atgccgatac tatcggcaat attagaagca gtcagacgac cttcatctcc atccaatgct
    13801 tcaaagatag cttgtacagc cttcaattcg ctataagata atgtgttgat tgccatttgg
    13861 acagccgttg cactccgtac atctgcttcg atgcttcgag acttctgata aaggatctgc
    13921 ataccgacaa ccgtagctcc atattctgct aagactagat cgtcatctgc aaacgattgt
    13981 tccatacgag ataagataat cgtacccaaa cgttcgccag ctccaaagat cggaacgaca
    14041 gtcgtcagac caaatggata tttctctctt gactctaccg gaaatgcagt aatatcacta
    14101 gtaattggga tattagcttc agttttcatc aactgtgcca ctgtatcagt ataagattca
    14161 ggaaattgtt tctcaacaaa cattgttttg attcgttcat tgttcacatc gtgacgttca
    14221 ttataaccta gtaatactcc gtcacttcct atgatatagg tgttgctgtc aagaatacca
    14281 cctagtgtga cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt
    14341 tgctgcaaaa gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc
    14401 cgatttttta taaaatatag cgacttaaat cttcatttgc cgcaattgag ccaagtttgt
    14461 catccacata tgcttctgtg atcgttattt ctcccatttg catatctggt gcttcaaata
    14521 atagatcttc tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg atattatctg
    14581 tgtcacggtt gacatcgaaa gcgatttgag cgatccgttc aattgcttct tttgtaaacg
    14641 taactgacac attttctgta ccgatcaacg cgacgtattg ttttactaaa gcattatttg
    14701 gttcggtcag aatcttcaca aaatcttcag ctgtcaaatc atctaattct acccgtatcg
    14761 ggaagcgtcc ttgaagttct ggaattagat cacttggttt agacaaatgg aaagctcctg
    14821 aagcaatgaa taggacgtga tctgtttgga tgatcccgta ttttgtgttt acttgtgaac
    14881 cttcgacaat tggcaatatg tctctttgga cgccttctct ggatacttct ccagattgct
    14941 gtgatttaga agtgatctta tcgatttcat cgataaaaat aattccactt tcttccgcta
    15001 atttgattgc ctcactatgg atatcagcat ccttgactaa ttttgctgat tcttccttga
    15061 tcaataactc taaagcttct ttgactgtta ccgtccgttc cacttttttc ttcggtgtca
    15121 aagcacctag agtttcattt aggtcgatcc ccatttgctc caatccattg ttcatcattg
    15181 gtgcaggttt cttttggtct tcaacttcaa tggttacttc acgactatcc agtaaaccct
    15241 tttgtagttg ttccaagact gtacggcgat tgattcgaat atcttctgtc acttcttctt
    15301 gcggttcagc ggtattttgc tgactagcat taaaaagatt catcatttgt tcaaattgat
    15361 tagtggattg tttctgttct tttttgatac caggtaccaa gatttttacc aaacgttttt
    15421 ctgcattttt tttagcactt gtgtatacac ggctgtactg ctctttttct acgatttgga
    15481 tcgcattttc tactaaatcc cgaaccattg attctacatc tcttccgaca taaccgactt
    15541 cagtaaattt tgttgcttcg actttgacaa atggtgcatt gacgattttt gccaaacgtc
    15601 gtgcgatttc tgttttaccg acccctgtag gtccgatcat cagcatattt tttggtgtaa
    15661 tatcttgttg cattttttca tctaactgca aacggcgata gcggttacgc aaagcaacgg
    15721 caacagattt ttttgctgcg tgctgtccta cgatatattc atctaattca cttacgattt
    15781 gtcttggtgt ttgttttaat tcatacatat ctactcacct cttacagttc ttctacgata
    15841 atattatgat tagtaaagat acagatatct gcggcaatat ttaacgcatt ttctgcaatt
    15901 tctttcgcac tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc agccaacgca
    15961 aaattccctc ctgatccgat agctaagata ccatcatctg gtgtgataac ttctcctgta
    16021 cctgaaacaa gaagcatttc tttatcattc attacgatca gcatcgcttc aagtttttgc
    16081 atagattgct gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg tagattcccg
    16141 ttatattcgt ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc tgcaacactt
    16201 ccggcgaaac ctacaacaac ttctccattg tagatacgtc gtactttctt tgcagtgcct
    16261 ttcatgacaa cagattcacc cattgttact tggccatctc cagccatagc aaatttcccg
    16321 tctttttcga cggcacagat agtggttgaa tgaaatggtg attcgaccat gtttgtttcc
    16381 tcctaaattt gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt
    16441 tgtaaacttt cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct
    16501 agcaattctt ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta
    16561 tggcgcagca tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc
    16621 aagacatatt caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata
    16681 tcatgctctt cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc
    16741 agagcatctt gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg
    16801 atcaacatga cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt
    16861 aaacggatgc ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga
    16921 ctatctcctg ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc
    16981 agtctcaaat ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct
    17041 tctttgagaa gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt
    17101 ctgctatagc taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat
    17161 tccgccttca gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg
    17221 tcttcttcat atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc
    17281 aaaaattttt gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa
    17341 taatttagca cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct
    17401 aagtttgttc acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac
    17461 gcaagaaaaa gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg
    17521 ggattgcagt tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg
    17581 caattgcttc atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac
    17641 catagttggc attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtaagcca
    17701 tacttccaat agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg
    17761 cggcgttgat tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag
    17821 tcatctgacc tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca
    17881 attctggcga attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat
    17941 tttctaaccc aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct
    18001 gaaagcccac gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag
    18061 cataaggacg tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa
    18121 gcatcgtttt gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt
    18181 tttctttctc aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag
    18241 ctttgaattc ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc
    18301 gggacttcaa atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg
    18361 catcatagaa ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat
    18421 gagaagtcaa aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt
    18481 cgtttatcac tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag
    18541 aatcgcggtc aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga
    18601 ttactaccga attcaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac
    18661 tgtttcctct taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct
    18721 cagtcgattt gactggacgc atttcatata aacggacagg gactcctgct tgtgcgatct
    18781 gccaagcagc ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa
    18841 gaacctcttt tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact
    18901 tgtttaccgc ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg
    18961 cccactggtt tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat
    19021 agtctgtttt tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc
    19081 acaccgattt cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca
    19141 taaaacttcc catatttccc taattttatc accatcggat gtccacagac atcacaatcg
    19201 aatcctgctg gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt
    19261 tctttttcaa acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct
    19321 tcaattttat ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg
    19381 aagaattcac agattaatga attgacaatt tctcctagtt cagtcggctc aaatcttttt
    19441 tgtgtcaatt ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa
    19501 ggtcgcccta ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct
    19561 ggtggttgag taaaatgttg ttttggttca atatcaacag attggactac atcgccttct
    19621 tcaagttctg gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac
    19681 acttgcatga atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt
    19741 tgttctaatg tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa
    19801 cgtgaccaga tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca
    19861 tctggtgtac gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga
    19921 gcatttttga tctttcggtg atcatgtcta gaaaattctt ttccatacgt atcttcaata
    19981 aatgctgcgg cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat
    20041 gtaatcaagc cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct
    20101 accatcatgg tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta
    20161 cttgtagtga aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact
    20221 tcgaagtctg ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat
    20281 ttctttttct taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta
    20341 aaattaccgt cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg
    20401 tcaataataa tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc
    20461 ttacgccaga gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct
    20521 tgctgtgcat ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact
    20581 gcgtcttttg tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt
    20641 aggtacgcta aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa
    20701 actttttgag ctttttttgc tgcggctttc aggcttttga tgacatcgcc ttttccgcgt
    20761 atagaaatat aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt
    20821 aaatctctta tatggccaac actggccatt actttatagt ttcgtccaag atatttctca
    20881 attgtcttgg cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa
    20941 tggactcctt tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct
    21001 aatcatatcc attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc
    21061 tttaaattct gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa
    21121 atgatgacat ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct
    21181 tccaagatcc aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa
    21241 gacacatgtt cctttagaca aagctgcgat aatccgattt ctcattggaa aacaatgctt
    21301 tttcgggccg gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc
    21361 ttgcaagtca cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat
    21421 tgtccgtcca ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca
    21481 gccactgacg atcacatatc cagcttctat cactggtttt agtaaataat aaactgtttt
    21541 tttggctaaa gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa
    21601 agatagatca ccattataaa acagtacaat tggcggatag gtcaagtgaa gcagctgagg
    21661 gggatatttg ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt
    21721 agcgtgaatt tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta ttttacctat
    21781 cttaatgatg tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag
    21841 tgcccatttt ccttgactac tgattccttt tgtataagca agtttcaata aattttcttc
    21901 tatttgatac atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg
    21961 ctgtttttta ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa
    22021 ttccttgtcg ttcaagtcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga
    22081 aaccatatcc aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag
    22141 caatgatact tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct
    22201 gctgaatagg caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca
    22261 atttttctac tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga
    22321 ttgtctgctg atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat
    22381 aaagaagttc tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct
    22441 catttttcgg caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta
    22501 cttcatcaat gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa
    22561 acatttcttc atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag
    22621 ccagtgcttg ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat
    22681 ctattctctg tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt
    22741 ttttgctcca tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttgacgaata
    22801 tcatgcacga ctaattcgct gccacgatca tagtcttctt tatacccacg ttttgctgta
    22861 atatccatca gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga
    22921 tcgctgattc ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca
    22981 tctaaatgga caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct
    23041 tcatcttcaa atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct
    23101 aaacgaagcc actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc
    23161 ttgcctacta gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt
    23221 gcccgaatag cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat
    23281 gcttctttag ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct
    23341 aatgcaagat ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct
    23401 gccagatccg ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc
    23461 attggattac gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca
    23521 tatttcattt tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg
    23581 atggtcattg ttctctccat tcctttcata ccggaatatt ccccggcttt acttgttttt
    23641 ttctttttca atataaattc acttttttat tataaagaaa ttccatctat tagttaagtg
    23701 aaagtgaacc agtcctcatt ctatcacatc ttcacttttt tgatgatgca aatttttatt
    23761 ttggtttcta tatatgaatc taaatttttg acagcgtttc ctaaataaga gatgatagac
    23821 gaacaaaaca gaaaggagac gaaacaaatg aatagtatgg tttttgttaa ttttcctgtt
    23881 gctgatgtca atcgttccgt tgctttttat gaaaagctgg gttttaaaaa aaatgaagaa
    23941 ttttc
//
LOCUS       DACCXW010000034        23828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCXW010000034 DACCXW010000000
VERSION     DACCXW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.034"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(53..787)
                     /locus_tag="IVY71_001106"
     CDS             complement(53..787)
                     /locus_tag="IVY71_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7985173.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IVY71_001107"
     CDS             1114..1524
                     /locus_tag="IVY71_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7985174.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IVY71_001108"
     CDS             1517..2218
                     /locus_tag="IVY71_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7985175.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IVY71_001109"
     CDS             2218..3804
                     /locus_tag="IVY71_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7985176.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IVY71_001110"
     CDS             3823..4044
                     /locus_tag="IVY71_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7985177.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IVY71_001111"
     CDS             4041..4802
                     /locus_tag="IVY71_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7985178.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IVY71_001112"
     CDS             5182..5691
                     /locus_tag="IVY71_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7985179.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IVY71_001113"
     CDS             complement(5766..6305)
                     /locus_tag="IVY71_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7985180.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVY71_001114"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVY71_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7985181.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IVY71_001115"
     CDS             7372..8169
                     /locus_tag="IVY71_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7985182.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IVY71_001116"
     CDS             8197..8832
                     /locus_tag="IVY71_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7985183.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IVY71_001117"
     CDS             8852..9625
                     /locus_tag="IVY71_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7985184.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IVY71_001118"
     CDS             10033..10830
                     /locus_tag="IVY71_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7985185.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IVY71_001119"
     CDS             10808..11221
                     /locus_tag="IVY71_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985186.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IVY71_001120"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IVY71_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7985187.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IVY71_001121"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IVY71_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7985188.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IVY71_001122"
     CDS             15998..16558
                     /locus_tag="IVY71_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7985189.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVY71_001123"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVY71_001123"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7985190.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IVY71_001124"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IVY71_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7985191.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IVY71_001125"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IVY71_001125"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7985192.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IVY71_001126"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IVY71_001126"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7985193.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IVY71_001127"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IVY71_001127"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7985194.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IVY71_001128"
     CDS             21905..22483
                     /locus_tag="IVY71_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7985195.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IVY71_001129"
     CDS             22535..23017
                     /locus_tag="IVY71_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7985196.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IVY71_001130"
     CDS             23134..23508
                     /locus_tag="IVY71_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985197.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DACCXW010000035        23653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCXW010000035 DACCXW010000000
VERSION     DACCXW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.035"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..506)
                     /locus_tag="IVY71_001131"
     CDS             complement(<1..506)
                     /locus_tag="IVY71_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7985198.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="IVY71_001132"
     CDS             779..3250
                     /locus_tag="IVY71_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7985199.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="IVY71_001133"
     CDS             complement(3353..5089)
                     /locus_tag="IVY71_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7985200.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="IVY71_001134"
     CDS             complement(5086..5790)
                     /locus_tag="IVY71_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7985201.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="IVY71_001135"
     CDS             5921..6424
                     /locus_tag="IVY71_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP7985202.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="IVY71_001136"
     CDS             6384..6722
                     /locus_tag="IVY71_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7985203.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="IVY71_001137"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="IVY71_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7985204.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="IVY71_001138"
     CDS             8273..8851
                     /locus_tag="IVY71_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7985205.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="IVY71_001139"
     CDS             8855..9376
                     /locus_tag="IVY71_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7985206.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="IVY71_001140"
     CDS             complement(9455..10009)
                     /locus_tag="IVY71_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7985207.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="IVY71_001141"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="IVY71_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7985208.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="IVY71_001142"
     CDS             10549..10800
                     /locus_tag="IVY71_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7985209.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="IVY71_001143"
     CDS             10925..11716
                     /locus_tag="IVY71_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985210.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="IVY71_001144"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="IVY71_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7985211.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="IVY71_001145"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="IVY71_001145"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7985212.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="IVY71_001146"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="IVY71_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7985213.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14014..14676
                     /locus_tag="IVY71_001147"
     CDS             14014..14676
                     /locus_tag="IVY71_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985214.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14755..17982
                     /gene="fss3"
                     /locus_tag="IVY71_001148"
     CDS             14755..17982
                     /gene="fss3"
                     /locus_tag="IVY71_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7985215.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18096..18410
                     /locus_tag="IVY71_001149"
     CDS             18096..18410
                     /locus_tag="IVY71_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7985216.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18423..18797
                     /locus_tag="IVY71_001150"
     CDS             18423..18797
                     /locus_tag="IVY71_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7985217.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18807..19151
                     /locus_tag="IVY71_001151"
     CDS             18807..19151
                     /locus_tag="IVY71_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7985218.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19222..20574
                     /locus_tag="IVY71_001152"
     CDS             19222..20574
                     /locus_tag="IVY71_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7985219.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20634..21776
                     /locus_tag="IVY71_001153"
     CDS             20634..21776
                     /locus_tag="IVY71_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP7985220.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21801..22055
                     /locus_tag="IVY71_001154"
     CDS             21801..22055
                     /locus_tag="IVY71_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985221.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22311..23495
                     /locus_tag="IVY71_001155"
     CDS             22311..23495
                     /locus_tag="IVY71_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7985222.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23510..23629
                     /locus_tag="IVY71_001156"
     CDS             23510..23629
                     /locus_tag="IVY71_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7985223.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
BASE COUNT         7832 a         4218 c         5073 g         6530 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    13681 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    13741 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    13801 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    13861 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    13921 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    13981 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    14041 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    14101 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    14161 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    14221 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    14281 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    14341 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    14401 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    14461 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    14521 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    14581 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    14641 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    14701 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    14761 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    14821 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    14881 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    14941 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    15001 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    15061 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    15121 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    15181 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    15241 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    15301 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    15361 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    15421 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    15481 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    15541 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    15601 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    15661 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacattg
    15721 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    15781 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    15841 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    15901 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    15961 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    16021 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    16081 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    16141 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    16201 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    16261 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    16321 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    16381 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    16441 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    16501 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    16561 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    16621 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    16681 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    16741 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    16801 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    16861 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    16921 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    16981 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    17041 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    17101 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    17161 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    17221 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    17281 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    17341 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    17401 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    17461 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    17521 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    17581 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    17641 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    17701 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    17761 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    17821 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    17881 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    17941 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    18001 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    18061 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    18121 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    18181 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    18241 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
    18301 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18361 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    18421 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    18481 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    18541 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    18601 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    18661 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    18721 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    18781 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
    18841 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    18901 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    18961 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    19021 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    19081 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    19141 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    19201 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    19261 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    19321 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    19381 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    19441 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    19501 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    19561 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    19621 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    19681 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    19741 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    19801 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    19861 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    19921 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    19981 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    20041 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    20101 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    20161 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    20221 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    20281 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    20341 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    20401 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    20461 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    20521 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    20581 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    20641 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    20701 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    20761 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    20821 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    20881 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    20941 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    21001 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    21061 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    21121 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    21181 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    21241 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    21301 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    21361 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    21421 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    21481 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    21541 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    21601 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    21661 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    21721 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    21781 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    21841 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    21901 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    21961 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    22021 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    22081 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    22141 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    22201 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    22261 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    22321 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    22381 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    22441 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    22501 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    22561 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    22621 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    22681 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    22741 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    22801 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    22861 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    22921 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    22981 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    23041 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    23101 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    23161 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    23221 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    23281 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    23341 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    23401 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    23461 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    23521 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    23581 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    23641 tcggtcaaaa ttt
//
LOCUS       DACCXW010000036        23117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCXW010000036 DACCXW010000000
VERSION     DACCXW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.036"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(166..756)
                     /locus_tag="IVY71_001157"
     CDS             complement(166..756)
                     /locus_tag="IVY71_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7985224.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            1240..1467
                     /locus_tag="IVY71_001158"
     CDS             1240..1467
                     /locus_tag="IVY71_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7985225.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1483..2649
                     /locus_tag="IVY71_001159"
     CDS             1483..2649
                     /locus_tag="IVY71_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP7985226.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2883..3926
                     /gene="hrcA"
                     /locus_tag="IVY71_001160"
     CDS             2883..3926
                     /gene="hrcA"
                     /locus_tag="IVY71_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7985227.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4020..4583
                     /gene="grpE"
                     /locus_tag="IVY71_001161"
     CDS             4020..4583
                     /gene="grpE"
                     /locus_tag="IVY71_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7985228.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4634..6463
                     /gene="dnaK"
                     /locus_tag="IVY71_001162"
     CDS             4634..6463
                     /gene="dnaK"
                     /locus_tag="IVY71_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7985229.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6614..7780
                     /gene="dnaJ"
                     /locus_tag="IVY71_001163"
     CDS             6614..7780
                     /gene="dnaJ"
                     /locus_tag="IVY71_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7985230.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7862..8527)
                     /locus_tag="IVY71_001164"
     CDS             complement(7862..8527)
                     /locus_tag="IVY71_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP7985231.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            8503..8676
                     /locus_tag="IVY71_001165"
     CDS             8503..8676
                     /locus_tag="IVY71_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7985232.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(8721..9458)
                     /locus_tag="IVY71_001166"
     CDS             complement(8721..9458)
                     /locus_tag="IVY71_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7985233.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9460..10962)
                     /locus_tag="IVY71_001167"
     CDS             complement(9460..10962)
                     /locus_tag="IVY71_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7985234.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11221..13215
                     /gene="uvrB"
                     /locus_tag="IVY71_001168"
     CDS             11221..13215
                     /gene="uvrB"
                     /locus_tag="IVY71_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7985235.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            13225..16041
                     /gene="uvrA"
                     /locus_tag="IVY71_001169"
     CDS             13225..16041
                     /gene="uvrA"
                     /locus_tag="IVY71_001169"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7985236.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16304..17569
                     /locus_tag="IVY71_001170"
     CDS             16304..17569
                     /locus_tag="IVY71_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7985237.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17573..18304
                     /locus_tag="IVY71_001171"
     CDS             17573..18304
                     /locus_tag="IVY71_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7985238.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            18439..19323
                     /gene="rapZ"
                     /locus_tag="IVY71_001172"
     CDS             18439..19323
                     /gene="rapZ"
                     /locus_tag="IVY71_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7985239.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19320..20318
                     /locus_tag="IVY71_001173"
     CDS             19320..20318
                     /locus_tag="IVY71_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7985240.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20352..21287
                     /gene="whiA"
                     /locus_tag="IVY71_001174"
     CDS             20352..21287
                     /gene="whiA"
                     /locus_tag="IVY71_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7985241.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            21830..22480
                     /locus_tag="IVY71_001175"
     CDS             21830..22480
                     /locus_tag="IVY71_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985242.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            22667..>23117
                     /locus_tag="IVY71_001176"
     CDS             22667..>23117
                     /locus_tag="IVY71_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP7985243.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNL"
BASE COUNT         7613 a         3939 c         4962 g         6603 t
ORIGIN      
        1 ataccagtat agcataatcc tgaatttttg aatccgtaaa accttgtcta tgaatataaa
       61 tagagtgtat cttctttgat aaatgtcaga aaaagtgtga caaataatca tcagtaaaat
      121 tacttgtcac actattgata aggagtattt aattgaagag aatgattagg agaaaggatc
      181 tccgtacttt ttatagtaat catagaatcc aagtttgtat agacgaatcg ttttttctct
      241 tatttcttct gtttcaaaat ggactgcttg aagaatcaag tctgtgaatt ctataggagc
      301 tgttccattc gcagtaatca aattacgatc tttcacagct tgttgttcta taaaatcttc
      361 tttattctga taattttccg attctaacca aggaaattgc gcatttccgg tgtgtttgta
      421 accttgcaat aatccgctct gagctaaata atcaactgcc ccacaaatag cagctactgg
      481 tacgtctgtt tttaacaatt tatcgattat ttgatataaa tgatgatttt ttatgtccca
      541 tgaattgcca ccgatcataa taaaaaggtc aactttttca ggtatttgtt cgattaagta
      601 atcgattttt gtttttaatc ctccaatgga agtgacctct gattggatag aagcagtttt
      661 gttttgccat ttctcatttt ggtttagtgt gctagtaata taagcacctt cccaatcggc
      721 atattcctct aatagaaacg taatcgcagt tttcattttc aagctctcct ttactatcta
      781 taacaaggac aacagtttta acctacagac attcattaaa attgggtagc gacagcgaaa
      841 acaacaattc ttaagtttta gacagagtat acagatagtc aacttaatta aatacgaact
      901 aaataaaaat gacaggtaat catttttgtt taccagcatt ataacatttt gcatacatgg
      961 atgacaaaag aaccaattcc cacttgcttg agtacaatat taaaagaaac tggaaggcaa
     1021 aaagtaatta tagaaacgaa aaataaatca tatcgggcac tcagacatga aaacaaccct
     1081 agagatatat aactaggtaa ccagtttaat gaaagaaaaa atcatccaag aagtcgattc
     1141 ttggattttt tagtggattt gttcgccaaa tacgtttagt atgcgtatat gagaggttgt
     1201 aagcaaagcg ttgtggtctg agcacttaag gaggaatctg tgaaaatagc ttttcatatt
     1261 tttgcagatt ttgacttaat gccgaaggtc agctttgcga ttaccgttta tcgagaggct
     1321 gtaaaagcac cgtgtagctc cgatgacaat aagcaagcat ttttagagct aggtttcctt
     1381 gattctaaat atgcggctta tttcaatgag ttggtgcttg aacgcgattt taagaaggtc
     1441 gaaaacaggc acgacttttc gacgtaagga gaaagaattt caatgataga aacaataaat
     1501 cccgcagaaa acaaggtttc tgcgtacatt catatcccat tttgcgagca tatctgctat
     1561 tactgtgatt ttaataaagt ttttttagaa gggcagccag tagatgagta tgtggaaatg
     1621 ctgctgaatg aaatgaaaat aatgctagaa agtcatccga cagcgaattt agaaacactt
     1681 tacgtagggg gcgggacacc aacttcttta tctgctaaac aacttgatcg actgttatca
     1741 ggtgctagag agatcctgcc ttttcaaaat ggtaatgagt ttactgtaga agcgaatcca
     1801 ggtgatttga cccgtgaaaa acttcaagta atgaaaaact atggcgtcaa tcgtttatcc
     1861 atgggagtgc agacatttga taatcgctta ttgaaaaaaa ttggacgtaa acacaccgca
     1921 gaagatgtgt atcaaactat gcaattttta gaagcagaaa attttaccaa tgtaagcatc
     1981 gatttgattt atgcattacc gggacaaaca ctagaaggat atctggatac tttggaaaaa
     2041 gctttggctt tggatctgcc tcactattct ttgtactcat tgatcctcga aaacaaaaca
     2101 atgttcatga actgggtacg acaaggaaga ctcgaactgc ctgatcagga aacggaaacc
     2161 agaatgtttg aagagacgat tgaagcaatg gaaaagcatg gacgtcacca atatgaaata
     2221 agtaactttg ggctctccgg acatgaatct caacataacc tcatgtactg gaacaatgcc
     2281 cactattttg ggtttggagc tggtgctagc ggttatcttg gagatattcg ttaccgcaac
     2341 aaaggaccaa tccaacacta tttacgccca ttacgagcaa atgaacgccc ggtattggaa
     2401 gaagaaaaat tgacacgaaa aaatcaaata gaagaagaaa tgttcttagg actaagaaaa
     2461 aaaacaggga ttactaaaaa acatttctat gatcgctatc aaaaaacaat tgaatcggtg
     2521 tacggtgatg tgctaaggga attagagcaa gaagggttac ttgttaatga agaagaccgt
     2581 gtttatttga caccaagagg gacctttttg gggaacaatg tgtttgagcg ctttttgttt
     2641 gaaaaatagt ttaaagaata tttgtttttt atctgaattt aaataaaaaa agttcaaaaa
     2701 tacaataaag gactattgaa acgttgatat aactggattt acatcaatta tttagaatta
     2761 gcactcttgt gtttagagtg ctaaaaaatg gagtctttct cttgacagga gaagggcttc
     2821 ttgctatatt tataagtgga ttagcactta gaatatatga gtgctaatga gaggtgagga
     2881 agatgttaac aaagcgacaa aacgacattt tacgtctgat catccaacac tatacgagta
     2941 gtggcgtgcc cgttggttcc aaaacattga tgtctgaggg agtcaaggcc agctcagcaa
     3001 cgattcgcaa tgatatgaaa gcattagaag atgaaggatt gctgttgaag actcactctt
     3061 cttctgggcg gattccatct gctttaggct atcgttatta cgtggatcat ttgttaaaac
     3121 ctgctcgcgt agcagaagat gatttgctgc tgattcgtca gtctctaggc agagaatttc
     3181 atgaaatcaa tgagattatt aagcaatctg ctgagatatt atctgagctg acaagctata
     3241 cgactttttc attgggacct gaaataaaag accgccgact gactggcttt cgtatcgtac
     3301 ccttgaataa tcgacagatt attgctatcg tcgtaacaga tatggggaat gtagaaagcc
     3361 aagtgttcac tcttcccgaa tatatgggaa gtcaggaact agaaaaaatg gtacagattg
     3421 taaacgaccg tctagtcgga gatcctctga tgacggtgta ccataaactt cgtacggaga
     3481 ttccgatgat tcttcataaa tatttccaaa cgacagaagg gatgtctcag ttatttgaca
     3541 ccgttcttgg tcatgttttt gaagataagg tttatgtcag tggacaaatg aatctcttgg
     3601 attatgagcc ccgtcaagat cttgatcagt tcaaatcgat gttttctttt atgaaagact
     3661 cagacggact tacacaaatg atcactccga tggatagcaa tattcatatc aagattggtg
     3721 ctgaattagg caatgattta ttgcagaata tgagtatgat tcaagcaagt tacgagatag
     3781 atggtcatgg aagaggtaca atagccttgt tagggcctac tagtatgcct tattctcgga
     3841 tgttcggtct agtcgatgtt tatcgaagag aactagcagc taaacttgct gattactacc
     3901 gttcgcttga cttgtcaaac tcgtaatgtg aataacgaag ctatttgcag ctgatcgttc
     3961 tctctttctt agtaagagca atcaaacagc tgactgaata atgaaaggaa gactgtatcg
     4021 tgaaagaaaa tcaagaagaa ctagataaag aaatgacaga tgctcagcca gaaccggaaa
     4081 tcgatgtcga agctgcagaa gattcgggta tttcagaagc tgaagcggaa gaattcgaaa
     4141 cagcgaaatt aaaagctgag ttagaagaaa tggaagataa atacttgcgg gctcgtgctg
     4201 aaatcgcaaa tatggcaaat cgaggaaaaa atgagcgtga gcaattacaa aaataccgtt
     4261 cacaagattt agccaaaaaa ttacttccat caattgacaa cctggaacgt gcattagcca
     4321 cagaagtgtc agatgatcaa ggggcaggtt tgaaaaaagg cgtggaaatg gtcttggaaa
     4381 gtttgcgtaa cgcactggaa gaagaaggaa ttgaaaaaat ccctgctaaa ggagaagcct
     4441 ttgatcctaa tcttcatcaa gcagtacaaa ctgttccagc gactgaagat acaccagccg
     4501 atacgattgt agaagtacta caagaaggct acaaattaca tgatcgcgtg ttaagaccaa
     4561 ccatggtcat tgttgcacaa taattgtgcg gatataaaac ttacactttc aattaaagaa
     4621 ggagacgtta attatgagta aaattatcgg tattgactta ggtacgacta actctgcagt
     4681 agcagtattg gaaggaaacg aagcaaaaat catcgctaac ccagaaggaa accgtacaac
     4741 accttctgtt gtatcattca aaaatggaga aatccaagta ggggaagttg caaaacgtca
     4801 agctgtgaca aacccaaata cgatttcatc aatcaaacgc catatcggtg aagcaggtta
     4861 caaagtagat gtagaaggta aatcatatac accacaagaa atctcagcaa tgatccttca
     4921 atatatcaaa ggattcgcag aagactattt aggtgaaaaa gtcgaaaaag cagttatcac
     4981 tgtgccagct tactttaatg atgcacaacg tcaagcaaca aaagatgctg gtaagatcgc
     5041 tggtctagaa gtagaacgta tcgtcaatga accaactgca gctgctcttg cctatggttt
     5101 agataaaaca gatcgcgacg aaaaaatctt agtattcgac cttggtggcg gtacttttga
     5161 cgtatctatc cttgaactag gagacggtgt ctttgatgta ttgtcaacag ctggcgacaa
     5221 ccatctaggt ggggacgact ttgataacaa aatcatcgac tacatggtag cagaattcaa
     5281 aaaagaaaac agcattgact tgtctcaaga caaaatggct ttacaacgct tgaaagacgc
     5341 tgctgaaaaa gcgaaaaaag atttatctgg cgtatcaagc acacaaatca gcttgccatt
     5401 tatcacagca ggagaatctg gaccattgca tttggaaatg acattgacac gtgcaaaatt
     5461 tgatgaatta acagctgact tagtagaacg tacaaaagta ccagtacgtc aagcgctaaa
     5521 agatgcaggt ttatcacaat cagaaatcga cgaagttatt ttagtcggtg gatctactcg
     5581 tatccctgca gttgtagaag cagtacgtaa agaaacagga aaagaaccaa acaaatcagt
     5641 taaccctgat gaagtagttg ctatgggtgc tgctatccaa ggtggcgtga tcactggtga
     5701 tgtgaaagat gttgttttac ttgacgttac accattgtca ttaggtatcg aaacaatggg
     5761 tggagtgttc actaaattga ttgaccgcaa tacaacgatt ccaacaagta aatcacaagt
     5821 attctcaact gcggcagata atcaaccagc agtagacatc catgtattac aaggtgaacg
     5881 cccaatggct gctgacaaca aaactctagg aagattccaa ttgacagata ttccagcagc
     5941 tcctcgtggt attccacaaa tcgaagtaac attcgatatt gataaaaacg ggattgtaaa
     6001 tgtatctgcg aaagatctag gtacacaaaa agaacaaaaa atcacgatca aatcttcttc
     6061 aggtctaaca gacgaagaaa tcgaacgtat ggtaaaagat gcagaagcaa atgcagaagc
     6121 agataaagct cgtaaagagg aagttgacct acgtaatgat gtagatgcat tgctattctc
     6181 tgtggacaaa acattgaaag aattagaagg caaagtggac gaagaagaag tgaaaaaagc
     6241 agaaacagct cgcgatgaat tgaaagccgc tgttgaagca aacaacatcg aagaaatgaa
     6301 aactaaacgc gatgcattga acgaaatcgt tcaaaacttg actgttaaac tatacgaaca
     6361 agcggcacaa caacaagctc aagaaaaccc agaagcagct caaggcggag cagatgatgt
     6421 cgttgatgct gattttgaag aagtcgatgg cgacgataaa taatcagttc actgaatttc
     6481 gagagaaggc caaagttgct actttggctt tttcttttga aaaatagttt gaagtatcgg
     6541 tttttgtttc aaacaaaata tgatatgata acaagcgacg tgaaaatgat gagaaccaat
     6601 aggaggaaag cctatggcaa caaaacgtga ttattatgaa gtcttagggc tctcaaaagg
     6661 agcatcagaa gatgaaataa agaaagctta tcgtaagctt tcgaaaaaat accatcctga
     6721 catcaataaa gaacctgatg cggaagaaaa attcaaagaa gtatcagaag catatgagat
     6781 tttaagtgac ccgcaaaaac gcgctgcata tgaccaatac gggcatgcag gcacagatcc
     6841 aaattacggt gctggtggag ctggtggcgg atttggtgga ttcggcggct tctctggtgg
     6901 cggcttcggt ggttttgaag atatcttcga ctctttcttt ggaggaggcg gtcggtcagt
     6961 cgatccaaat gcgcctcgtc aaggtgctga tttgcaatat acggtcgact tgtcttttga
     7021 agaagcgatt tttggtgtag aaaaagaaat caagtataat cgggaagaaa tctgtcatac
     7081 ttgtggtggg aacggtgcaa aaccaggcac acaaccaact acttgtcata aatgtcatgg
     7141 tgcaggtacg attaatgtag aacgccaaac accacttgga cgagtgatga gccgtcaaac
     7201 ttgtgatgtt tgtcatggga cagggaaaga aatcaaagaa ccttgtccaa cctgtcatgg
     7261 ctcaggacat gagaagaaag cacattcagt taaagtgaat gtaccagctg gtgtagaaga
     7321 aggacaacaa atgcgtttag ctggtcaagg agaagctgga gaaaacggcg gaccatttgg
     7381 tgatttgtat gtggtcttcc gtgtagaaga aagtgatatc tttgatcgtg aaggttctga
     7441 aatttactat gaattgcctt taagttttgt acaagcagct ttgggggatg aagtccaagt
     7501 accaactgtc catggagatg tgaaattgaa gatccctgct ggaacacaga caggtacgaa
     7561 cttccgttta cgtggaaaag gcgctccgaa gttacgtggt ggtgcaacag gcgaccaaca
     7621 tgtcaaagta aaactgatca caccgaaaaa tctaaatgaa caacaaagag aagcattacg
     7681 tgcattcgct gaagctggcg gcatcaaagt tgaagaacag caagaagacg gtttcttcga
     7741 taagtttaaa gatgcttttg gcgggaaaaa gcggaagtag tttttctagt aaaaacgcta
     7801 agtcattaat aattaaaaac cagacgaaca atcatatgtt cgcctggttt ttttgtagtt
     7861 gtcatgtttg taccaacaaa aaagccagct ttagaatatg ataatcgtca tagtttctag
     7921 gggtataacc agtgagagaa tggaacttgt tcaatcgata ttgcaaggtg tttttatgaa
     7981 taaataattc ttctgagcaa tttttgatgc ttccgttatt tttactgaag catgccaaag
     8041 tcttcttgaa caaatgtact tcttcttctg tcaggctttt caaaatacgt tttacatata
     8101 atgttcgttg atctgacggg attgctggta aaacgatccc gaggtctaaa tcttcaaaaa
     8161 agcggatctc gttcacagat tgatggaaag ctatccattc gcaaacttgt ttggcatgaa
     8221 ggaaagattg cggtgtctct tgctctgtat aacaaagcga cccaattcca aagtttagct
     8281 gtaactgata cattttatta aactttgtct ggattttatc aagcaatgat agcaatgagg
     8341 ctctggaaat ccgattgact aggataatga tttgttgtct atgtacagtg agcaaagcct
     8401 gtccgtaata agagaagacc ctttcgagca actccaataa aggattgatc cgttcttgac
     8461 acaattgctg attgtcagaa agttgtccca caactacata atatggagaa gaaaaatcaa
     8521 gctcaattaa gtgagggtgg tatcgactat gtacttgata tcatcaatga accgatggta
     8581 ctttcttatg cgatggaaca ggtagagcga ttgcttgttt gtgcaggaaa acaagcgatg
     8641 cggctgatac gattaggtga gaaaataaga agataaaaat acacggtccc ttcatggcga
     8701 ccgtgcattt ttggttttta ttaaatattt aatactttat tcaagaaatc ttttgtccgt
     8761 tcattttgcg gattttcaaa gatttgttga ggcgttccat cttctaaaaa gtttccgtcg
     8821 tcaatgaaca tgacgcgatt ggccacttct ttagcgaacc ccatttcgtg cgtaacgata
     8881 accatcgtca taccttgttc tgctaacttt ttcatgacgt tcaagacatc accgaccatt
     8941 tcaggatcga gtgcagaagt aggctcatca aaaagcatga tgtcagggtt catcgctaga
     9001 gcacgagcaa tagccacacg ctgtttttgt ccaccagaaa gcatttcagg atacatatct
     9061 tttttatctt ctagaccaac tgtctgcagt aatttgattg cttttttctc tgcttcttct
     9121 ctagattctt tcttcaggtc catcggtgct aaaatgatgt tttccaaaat agaaagatga
     9181 gggaataggt taaagtgttg gaataccatt ccgatatgtt gtctcaccaa gttgatatct
     9241 gtgcttttat ccatcaagtg ggcgccgtca ataataattt caccgtcaga aggttcttct
     9301 aaacgattca agcaacgcaa gaatgtactt tttcctgaac ctgaagggcc aatgatacag
     9361 acgacatctc cttcgttgat ggatacattg atgtcgttta atactgtgtt atcgccgtat
     9421 tttttgacta aatgctcaac taagattttt tctgccatct tacttcacct tcttttctaa
     9481 aacttttgct aattttgtca atgctgtgat caaaatcaag tacatgatcg caatgatcaa
     9541 gtaaacatac gtactttgtg ttgtacgtgc aacaatgatc ttaccagttt gcagcaattc
     9601 gactacaccg attgcggaaa tgatcgttgt gtcttttaat gagataacga actgattgac
     9661 gaatgatggg atcatgatgc gtatcgcttg cggcaaaatg attttttgca ttgtccgatt
     9721 ataagaaaga cctaaacttc tggaagcttc catttgtccg acaggaacgg cgttgatccc
     9781 gcctcgaaca atctctgcga tataagcact tgcgttaaga gtcaaggtga taacaccagc
     9841 aaggaagtca ggaatcgtaa atccaacgat accaggcaac ccaaagaaaa taaagaacgc
     9901 aagaaccatc attggaattc ctcggatgat atcgacataa atactagcaa ttgtgcgaag
     9961 agcgcgtatt ggtgctacac tgaataatcc aaaaatcaca ccgattacta tagccaaagc
    10021 aaaggagatc aaagttagga gaattgttct ccaaagtcct ttcaacaatg cttgatagtt
    10081 gttttggata agtccaagaa atgttgattc atcagcagtg gaagactcac tggaagtttc
    10141 tgaaccatcc gctacatatt ctttaataat cttatcgtat tcgcctgttc tttttaattc
    10201 tttcaatcct tcattgaaca tattaagaag ctcaggattt tgtccttttt taacggcaaa
    10261 tgcatatttt ccgccagatt cttttttgat cggtgtttcc aattcttgtc cttgtttgat
    10321 cgcataaccg attaccggat aatcatccat cattgcatcg attgcgccaa cttttaaccc
    10381 gtcgtacaat tgatcagcag catcaaaata tttgatagaa tagccatatt cagattcatg
    10441 gtcgcttaaa aattcagcac tttcggtacc aacttttgct ccgactgttt ttcctttcaa
    10501 atcttcataa gacttgatcg aagtatctcc ttttttgaca gctaattgga tcccgctatc
    10561 aaagtaagaa tcagaaaaat catagctttt ctttcgttca tctgttacag tcattccggc
    10621 aatcattccg tctgcttgtc ctgattccaa agcttgcaaa gcagcagaga aaccacggta
    10681 gtccattcgg atgttaaatc cttgcagttt ggcgatgttt tccatcaatt ttacatcgat
    10741 gccttcgtac tcgccctttg cgttttgaaa ttcaaatgga gcaaaagcag agtcactggc
    10801 aattacgtat acgtcttttt tgggctctat tttttgcatt gtctcgcttg tgcttgactc
    10861 tgccgttgta gtgttgtcag cttgtgcaac atgaccaaac gcaagtgtca caagtcctgt
    10921 cattaagaaa acaaatgagt gaaataaatg ctttcttttc ataatcaacc tcctaaattt
    10981 ccaatatttt attattatag gcgaaaagtg ctgaatcaga caagactgta agctcgaaaa
    11041 aatgaaaaaa atgcgagttt ctaacatttt atgttataaa acataacatg tataataaga
    11101 taaaaaagtg gcgaaagtat gttcggtgtg gtataatttt ttagtaagga atgcctttgc
    11161 tttccttttc tttttattgg tcgtaaaatg aaaaggaatg aatgaaaagg aggacgaaga
    11221 atgattgaga gagatacctc ccgacacttt gagcttgtct cgaaatacca accggctggt
    11281 gatcagcctg aagcgattaa ccaattagtg gatggcgtag tcggcgggaa aaaagcacaa
    11341 atcttattag gtgcgactgg aacagggaaa acatatacga tttccaatct tattgagaaa
    11401 gtgaataaac caacactgat tattgcccat aacaaaacat tggctggtca gctgtacggg
    11461 gaattcaagg aatttttccc taacaatgct gttgaatatt ttgtttctta ttatgattat
    11521 tatcagcctg aagcttatgt gccttcaagc gatacgtata tcgaaaaaga ttcaagtgta
    11581 aatgatgaga tcgacaaatt acgacattcg gctactagtt cattattaga aagaaatgat
    11641 gtgatcgtca ttgcatccgt ttcatgtatt tttggattag gctcgccttt tgagtaccaa
    11701 aaacaagtag tttcgattcg acagggagca gaactagatc gcaatcagtt gattcgtgac
    11761 ttagtcagta tccaattcga aagaaatgat atagattttc agcgcggtcg ttttcgagtg
    11821 cgcggtgatg tcgtggagat ttttccggct tcaagagatg aacgtgcctt acgtgtggag
    11881 ttttttggag atgaggtcga acgtatccga gaagtcaatg cactgacagg tgaagtttta
    11941 ggagaaacgg aacacgtagc aatcttcccg gcgactcact ttgtgacgaa tgatgaacat
    12001 atggagcatg ccgtagcgaa tatcaaagcg gaattagaac agcgtttaac tgtcttgcga
    12061 aatgaaaaca aacttctgga agctcaacgt ttagaacagc gaaccaatta cgatattgaa
    12121 atgatgttag aaatgggcta cacgtcggga attgaaaatt attctcggca tatggacgga
    12181 cgtaaagaag gtgaaccgcc ttatacgcta ttggatttct ttcctgaaga ttttttgatc
    12241 gtggcggacg aatcgcacgt aaccatgcct caaatcagag gaatgtataa tggtgaccga
    12301 gcgagaaaac agatgctagt agattacggt ttccgtttgc catctgcttt agacaatcgg
    12361 ccacttcggt tagaagagtt tgaaaaacat gtcaatcaga tcatttatgt ctctgcgacg
    12421 ccaggaccat atgaacatga acaaacagat accgttatcc aacaaatcat ccgtccgaca
    12481 gggctgcttg acccagtgat cgaagttcgt ccaatcatgg gacaaatcga tgatctagta
    12541 ggcgaaataa atgaacgagt cgaaaaagac cagcgggtgt ttgtaacgac attgacgaaa
    12601 aaaatggcag aagacctcac agattatttc aaagaattag ggatcaaagt caaatatttg
    12661 catagtgata tcaaaacgct ggaacgaaca gaaatcattc gagatcttcg attaggtgaa
    12721 tttgatgtgt tagttggaat caatttgctt cgtgaaggat tagacgtacc tgaagtttcc
    12781 cttgttgcta tcttggatgc ggataaagaa ggctttttac gtagtgaacg ttccttagtc
    12841 caaacgattg gacgagctgc tcggaatgaa gaaggtaaag taatcatgta cgcagacaaa
    12901 gtaacagatt ctatgcgtct agcgatggat gaaacgtcaa gacgtcgtac gattcagcag
    12961 aagtacaatg aagaacatgg gatcgttcca aaaacgatca ttaaagaaat ccgagatttg
    13021 atttctatta cgaaagaatc ggaagatgat acaaaagaag cggtacaagt ctcgtacgaa
    13081 gaaatgacga aggaagaaaa agacacgtta ttgatgaaat tagaaaaaga aatgaaagat
    13141 gcggcaaaag ctttggattt tgaaacagcc gcgaatgtgc gagatatgat tttagaatta
    13201 aaagcttcaa agtaggagga caacatggct aacgataaaa ttacgattca tggtgcacgt
    13261 gcccataact tgaaaaatat agatgtaacg attccacgag ataaattcgt ggtcgttaca
    13321 ggtttgtccg gttctggaaa aagttcccta gcatttgata cgctgtacgc tgaagggcag
    13381 cggagatacg tagagagcct ttctgcttat gctcgtcagt ttttaggaca aatggataag
    13441 ccggacgtag atagcatcga cggattgagt ccggcgattt ctattgatca gaaaacaaca
    13501 agtaaaaatc ctcggtcgac agtaggtact gtgacggaaa tcaatgacta tttacgcttg
    13561 ttgtttgcac gtgtaggaca tccgatttgt cccaacgatc atatcgagat taccagtcaa
    13621 tcagtagatc aaatggcaga tcaagtaatg gctttgcctg agcgcacaaa gatccaagtg
    13681 ttggcaccga tcgtggtaaa gaaaaaagga caacataaaa aaatattcga acgaatccaa
    13741 aaagagggat acgtccgcgt tcgagtcgat ggtgagatct atgatttaag tgaagtgcct
    13801 gagctggaga aaaacaaaaa acatgatatc gcgatcgtca tcgaccggat cattgtgaaa
    13861 gaagggattc gttctcgcct atttgattct ttagaggcgg ccctgcgatt agcagacggc
    13921 tatgctctgg ttgacgtgat cggtgaagaa gaaatgttgt ttagcgaaca ctatgcctgt
    13981 ccttattgcg gatttactgt tggcgaacta gaaccaagac tgttctcatt caatgcgcct
    14041 tttggagctt gtccagactg tgatggttta ggtgtcaaat tagaagtcga tcaagattta
    14101 gtgattccag atagaacgaa gacacttagt caaggtgcta tcgttccatg gaatccaatc
    14161 agctctcagt attatcctca aatgttagcg caggcttgtg aaagtttcgg tatcgattta
    14221 gatacaccat tcgaagaatt accggaagag catcaggaga tcatcttgaa tgggacagat
    14281 gatcttttcc atttccatta tgaaaatgat tttggtggcg ttcgcgatgt agaaacgact
    14341 ttcgaagggg tattgaagaa tatcgagcgt cgttatcatg atacgaacag tgatttcacg
    14401 cgtgagcaaa tgcgtcttta tatgacagaa ttaacttgtc agacatgtaa aggttatcga
    14461 ttgaatcccc aagctctatc tgttcaaatc aatggaacga atatcgggca aacaaatgag
    14521 ttgtctattc aaaaagcagt agatttcttc tcaaacttaa cgttatctga acaagagaaa
    14581 gtaattgcca aaccgatttt aaaagaagta aatgacagac tttcattctt ggaaaatgtg
    14641 ggactagact atctaacttt gagtcgcgct tccggaacat tatctggggg agaagcgcag
    14701 cgaatcagat tggctacaca aattggatcg aatctttctg gagttttgta tattttagat
    14761 gaaccatcga tcggattgca tcaacgcgac aacgatcgac tgattggctc attaaagaaa
    14821 atgcgtgatc ttggcaacac cctcgttgta gtcgaacatg atgaagatac gatgtgggca
    14881 gcagattatt tgattgacgt cggacctggg gccggagaac aaggcggaca aatcgtggct
    14941 gcaggcactc ctgaagaagt agctaatgat gaaaattctt tgacagggaa atatctttca
    15001 gggaaaaaat caatccctgt accaaaagaa cgccgtaaag gtaatgggaa agcaatcaag
    15061 atcactggtg cttcagaaaa caatctgaaa aatattagtg ttgaatttcc attaggagaa
    15121 tttgtagcag tcacaggtgt gtctggttct ggaaagagta cattggtcaa tagtatctta
    15181 aagaaatcgt tagcgcaaaa attaaataag aattctgcta agccaggtaa attcaagaca
    15241 atttccggct acgaaagtat cgaaaagatc atcgatatcg atcaaagccc aatcggccgg
    15301 acgccgagaa gtaatccagc gacttataca agtgtatttg atgatatccg tgggttattt
    15361 gctcaaacga acgaggcaaa aatgcggggt tataagaaag ggcgttttag tttcaacgta
    15421 aaaggcggtc gttgtgaagc ttgtcgcggg gatggaatta ttaagatcga aatgcacttt
    15481 ttgcctgatg tctatgttcc ttgtgaagta tgtcatggta aacgatataa ttctgaaaca
    15541 ttagaagtgc attacaaagg aaaaagcatt gctgatattt tggaaatgac agtagaggat
    15601 gctgtagaat tcttcaagca cattccaaag attcatcgca aactgcaaac gattgttgat
    15661 gttggcttag gttatgtgac gatggggcaa ccagcaacga cattgtccgg tggtgaggca
    15721 caacggatga aacttgccag tgaattgcac aaaatctcta atggaaagaa tttctatata
    15781 ctagatgaac caacgacagg acttcatagc gatgacatcg cccgcttgtt gcatgtatta
    15841 caaagattag tagatgctgg taacacagtt ttagtgattg aacacaatct agatgtaatc
    15901 aaaacagcag attatatcat tgatttagga ccagaaggtg gagaaggtgg aggaacgatc
    15961 cttgcgactg gaacaccaga agaaatcatt aacgtaaaag aaagttatac aggtcactat
    16021 ttgaaaaaaa taatggtata aagaggatag atagctgaga tagttaaaaa aacataaaaa
    16081 aatgaatttt tgcttgtttt attaatatca gtgttctaga atcattacat ggtagttctt
    16141 tctacactga ttggtataga taaaaacttc ctataagttc aatccgttca aaaaagaatt
    16201 gagcaatcgg aggactttcc tttgattgct tttttcttta tggtatgata ttccttgtgt
    16261 agttggaaaa gaaaaacaag agattatgag taagtgagag ataatgatga acagtattga
    16321 aaatgaagaa tttatcccta tattattagg aagcgacatg aacgtttatg gtatggcacg
    16381 ttcttttaat gaagcatacg gcaaaatctg ccaagcatat gcttcggatc agttagcccc
    16441 tacccgctac agtaaaattg tcaatgtaga agttattcca ggatttgata aagaccctgt
    16501 atttatcgag acgatgcttc gtttggcaaa agaaagatat tctgataaga gtaaaaaata
    16561 tcttttgatt gcttgtggtg atggatatgc agaattgatc tcccaacata aacaagagct
    16621 gtcagaatat tttatctgtc catatattga ttattcttta tttgaaaggc tgatcaataa
    16681 agtaagcttt tatgaagttt gtgaagaata tgatcttccg tatcctaaaa cattgatcgt
    16741 cagagaagaa atgttagtaa atggacattt ggaacaggaa ttgccttttg aattcccagt
    16801 tgctttgaaa cctgcaaaca gtgtggagta tctttctgtc caatttgaag gaagaaaaaa
    16861 agcttttatt ttagagacta gagaagagtt cgatttaatt ttaggccgga tatacgaagc
    16921 cggttataaa agtgaaatga ttgtacaaga ttttatcccg ggtgatgata gtaatatgcg
    16981 tgtgctaaat gcgtatgtag atgaagatca tcaagttcgg atgatgtgcc ttggtcatcc
    17041 actattagaa gatcctacac cagcatcgat tggtaattac gttgtgatca tgccagatta
    17101 taacgaaaaa atctatcaaa ccattaaggc atttttggaa aaaatcgaat atacaggctt
    17161 tgccaatttc gatatgaagt atgatcctag agatggagaa tataaattat ttgaaatcaa
    17221 tttgagacaa ggtcgtagca gtttctttgt gacattgaac ggcttgaatc tggctcgttt
    17281 tgtcacagaa gatcgagtgt tcaacaaacc ctttgttgaa acgacctatg gaacgaatca
    17341 atcagataaa gcaagattat ggatgggtgt gcccaaaaaa atctttttag aatatgctcg
    17401 ggaaaatgaa gacaaaaaac tagctgagca aatgatcaaa gaaaatcgtt acggtactac
    17461 tgtattttat gaaaaagacc gatcaatcaa gcgctggttg ttgatgaaat atatgttcca
    17521 taactatatt ccaagattca aaaagtattt tcatgtgaaa gaaggctagt tcatggagaa
    17581 ttttttcagt attttaggcg gaatgggcac gatggcgaca gaaagttttg ttcgcttgat
    17641 caaccaccga acgaaagcta caaaagatca agaatatttg aattatgtct tattcaatca
    17701 tgcaacagtt cctgatcgta cagcttatat tttagatcga tcagaagaaa atccaatgcc
    17761 gtttctgctg gatgatattg agaaacaaaa tttattgcga ccaaacttca ttgtgttgac
    17821 ttgtaacacc gcacactact tttttgaaga gttacaagcg gcaacggata ttcctatctt
    17881 gcatatgcca agagaagcag caaatgaact tgtccgtcag catacgacag ggagagtagc
    17941 gattctagga acagaaggaa gtatgaaagc tggaatctat gaacgcgaag tcaaaaatct
    18001 tggtttcgaa acagtgattc ctgatactgc gcttcaagaa aaaatcaatt acctgatcta
    18061 tcatgagatc aaggaatcgg attatttgaa tcaagagctc tattacgaaa tcttagagga
    18121 agcggtagag cgcctaaact gtgaaaaagt catcctaggt tgcacagaat tatctttgat
    18181 gcatgaattt gctgaagata atcattaccc agtgatcgat gctcaatcta ttttagcgga
    18241 tcgaacgatc gaacgtgcat tagctgaaag aagcgaggca ttggatacag catcggaaaa
    18301 gtaaaaaaat tggatttggg gcaaatatcc taaaagatat ttggtccaaa tctttttgta
    18361 aaagaaaaag aaaactggta atgatacgcc cgggatgata taattttatt atgcacaatg
    18421 tgagaggggt tcgtaaatat gacagataat ttgcaattag tcatcataac aggtatgagt
    18481 ggtgcaggga aaacagtagc tatccaaagt tttgaagata tgggctattt ttgtatcgat
    18541 aatatgccgc ctcgattgat tccaaaattc tgggaattga tcaaagaatc aggtaaagtc
    18601 acaaaaatgg ctttaggagt ggatttgcgt tctcgttcct tctttgaaga aatccaaaac
    18661 atgctgatcg aaatcgaaaa tacgaacttc atcgacactt ctatcttatt tttagatgct
    18721 tcagacgaag aactagtggc tcgctacaaa gaaacaagac gagcacatcc aatggcgatg
    18781 gacggtttga tcactgaagg gatacgtaaa gaacgggcga tactagacga cttgaaagcc
    18841 caagcttccg tgatcattga tacgacaaca ttgacaccac gacaattacg tgaaaaaatc
    18901 aatgagtcat tcaaatcaaa gggagaccaa ggatttcgga tcgaatttgt atcatttggt
    18961 tttaaatacg ggttgccgat cgattcggat attgtgatgg atgtgcgttt tttgcccaac
    19021 cctcattata tcccggaact aagaccattg accggaaaag atcagccagt ctatgattat
    19081 gtgatgtcct ttgatgaaac gcaatcattc taccataaat ttttggatct tctggaaact
    19141 gtattgcctg gatacattaa agaaggaaaa agcagcttga cagttgcaat cggctgtaca
    19201 ggcggtcaac accgttctgt ggcattgact gaacgtttgg caaatgcttt agggaaaaac
    19261 tataaagtca atgtgacaca tcgggacaag gataaaagga aagagacggt gaaccggtcg
    19321 tgaaaatgaa aacctaccgg ataagaaaac caaagatcgt agtggtcggc ggtggcactg
    19381 gtctgccagt tatcctgaaa agtttacgaa atcaaggagc agatatcaca gcagtagtga
    19441 ctgttgcaga tgatggtgga agcagcggtg caatcagaga atcgatcgct atggctcctc
    19501 ctggtgactt gagaaatgtc ttagtggcgt tatcggatat gccacaattc tatgaagata
    19561 ttttccaata ccgtttcaaa aaggaagatc agtttttagc caatcatacg atcggtaatc
    19621 tgatcattgc cgcagtttcc gagatgcggg gaagcactta tgaggcgatt caattattag
    19681 caaaaatgat gcatgtagat ggacatgttt atccttcttc agaaactcct ttgaccttgc
    19741 atgctgtttt taaagacgga acgactgcag taggagaatc taaaatcgcg atcaaccgta
    19801 aaaccattga ccgtgtgtat gtctgcaaca caaatgataa aagtgaagcc aaagcggctc
    19861 gaaaagtcgt ttcagcaatt atggacgccg acatggtcgt tttgggaccc ggaagtttat
    19921 tcaccagtat ccttccaaat ttagtgatac cagaaatcgg cgaagcaatg ctcaagacaa
    19981 ctgctgaaac cgtctatatc tgtaatatca tgacccaaaa aggggaaacg gaacatttca
    20041 cagatgccga tcatatacgt gttttacaca agcatttgaa tcagcctttt atcgatacag
    20101 tattggtcaa tacggaaaaa gtgcctgaag attatatgga cccagagatc tatgatgagt
    20161 atctagtaca agtcgcacac gacttcaacg gattgagaaa tgaaggatgt cgagtgattt
    20221 ccaccgactt tttagaattg aaagatggtg gtgtcttcca cgatggagac aaagtagtag
    20281 aagaattgtt ccgtttagtt ttcggttcaa aatattaagc aatttattgt tggagaaagg
    20341 aggagaatag gatgtcattt gctgcagatg tgaagaaaga actaactggt ttggaagttc
    20401 atcgagaaca tgcgaaagcg gaattagctg ctttgctgcg aatgaacggc tctttaggta
    20461 ttgtgaacca gcagtttatt ttgaacgtcc aaacagaaaa tgctgcaatt gccagacgta
    20521 tctattctct tttgaaagat cattatcagg tacgaagtga gctactcgtt cgtaaaaaaa
    20581 tgaaattaaa aaagaacaat gtctatatcg tccggttgaa gcaaggcaca aaagatgtgt
    20641 tgatggatct tgatatcatg gatggtatga tgttcaatgg acacgtttcg gacgagatta
    20701 tgggaaattc gcaaaaaatg cgttcttact tacgtggtgc ttttatggcc tcaggttcag
    20761 tcaataatcc ggaaacgagt cgttatcatt tggaaatatt ttctatttat gaacagcata
    20821 accaagacat ttgcgatatg ttaaattatt acggtctaca tgctcgaacg cttgaacggc
    20881 gcaatggcta tatttcgtat ttaaaaggag cagaaaaaat tgctgatttc ttaacattga
    20941 ttggtgcaac caactcaatg ctccgatttg aagatgttcg aatcgtacgg gatatgagaa
    21001 attcggtcaa tcgtctagtc aattgtgaga cagcaaatct gaacaagacg attgatgcgg
    21061 cttctaaaca aatcgaaaac atcgaattga tcgaagcaaa ggtcggtcta catgcccttc
    21121 ctgaaaaact tcaggaaatc gcagaacttc ggcttcaaca tccagaggtc agcttgaaag
    21181 aattagggga aatgattccc tcaggtgcaa tatcaaaatc gggaattaat caccgaattc
    21241 gcaaaataaa tgaatttgcg gaaaaactgc gtgaagaagc agtttaatcc aaagaaaaaa
    21301 ctgatacatg aagcaggaga tgcgaatgta tcagtttttt ttaaaagcta tacttgcttt
    21361 tcttccgtca aactataatt ccaactttac tggagtaaca taatgttgaa aggcttctat
    21421 tgttttttaa tacaatcgta taaaaagcgg ggagacaact gaatgaagat tttagtttta
    21481 tacataaact tttgcttaat atagaagtcc tgatatagat aggatcaagt atttatctat
    21541 aactggagat taattaaaaa ctggaaggaa ctgctcgatc aaccttgaga actgtttttc
    21601 ttacttctgg tcaagattct cgtggttggt ggataaggat ggttgataag ttcattggat
    21661 tagctagtcg aaaacaacca ggtgtaatta atgtagaagt tttaacaagg ggatggagtg
    21721 atttattcag tgataatcat aagttcataa atagtaattg ggatgtatgg ttggtatacg
    21781 ttatacctga acttgaacta tcaagatttg gtttagaatt tgagaatcga tgtttataaa
    21841 gggagggcaa aaaatgtgct tttttacaac ttttactatc attatgattg cagtactttt
    21901 tatttttcta gatatagcaa agcgaaatac agcattttta ttatatcggg ttttacttcg
    21961 agcaggtctc ataacattta tatcgatcgt tggatttttt ctattcacag ttattgtatt
    22021 tatttggaga actacagcac caccattgcc agagattaca tatggagaat ttccgtttag
    22081 tcttgaatat gaaatagatg aggaaagaat tttgattgaa gatacggtga ttgcagaatt
    22141 tataggaagt tttagaggga atgtgacaac tcaacctaga cgaagttgga gtataaaact
    22201 tttaaataat agtggagagt tgaaagcatt tcattatgaa ttcttaataa tagaaagtga
    22261 agatgtaaca atcactttaa gtagtggctc tgctggatat tttatgaatg atggtggaag
    22321 caagccacag ctcacagtcc gtgatcgtat tgcaggtttt atgcattttt ctcactatga
    22381 cttagaagaa gctcgtaaaa tacttgcaga atatgggatc acattaatca attgggaata
    22441 tgcgccaccg ataagaaatt ttttcaatga ttattattag tcttataaaa aacagaatgt
    22501 aaaatttatg tgcgcgagta tttagttttc cattttttgt ttgatagata tatagcattg
    22561 ttgaagcatg ggatagtggt gccaatttta tcaatgaggt tggtcaggca gttaacataa
    22621 ttggtgatgc tataaatcca tttaattgga attggaggaa ttaggtgtgg aaacagtgag
    22681 attatggata ttcatgaaaa attttcgttc ggctttgaga tatgttattc tggaatcaaa
    22741 atcaggttat tatctgattg acacagatca ctatactttt tggggaggct acctttttcc
    22801 gatttttaat tggataatcc cccataaggt tatgaaaatt aacttgcctg cagaagaaat
    22861 aaatagatta atactcaaca aagatttaca aaggaaaaga gagaaagaac attctaatgt
    22921 tgcttggata ggtccagcat ttgcagtagt gacttcttca attgtcggtc ctatgctaga
    22981 gagtatttat agtgattttt ctattggaat acgttattta tttgttactt taagtatagc
    23041 tgttcttttg tgcataaagg gttatgttag caagaagaat aaagatattt taacactaat
    23101 cggtgtaaac aatttat
//
LOCUS       DACCXW010000037        22636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCXW010000037 DACCXW010000000
VERSION     DACCXW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.037"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(263..715)
                     /locus_tag="IVY71_001177"
     CDS             complement(263..715)
                     /locus_tag="IVY71_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7985244.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(728..1138)
                     /locus_tag="IVY71_001178"
     CDS             complement(728..1138)
                     /locus_tag="IVY71_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7985245.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1413..1625
                     /locus_tag="IVY71_001179"
     CDS             1413..1625
                     /locus_tag="IVY71_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7985246.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1628..1840
                     /locus_tag="IVY71_001180"
     CDS             1628..1840
                     /locus_tag="IVY71_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985247.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(2132..2356)
                     /locus_tag="IVY71_001181"
     CDS             complement(2132..2356)
                     /locus_tag="IVY71_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7985248.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2434..2622
                     /locus_tag="IVY71_001182"
     CDS             2434..2622
                     /locus_tag="IVY71_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985249.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2948..3283
                     /locus_tag="IVY71_001183"
     CDS             2948..3283
                     /locus_tag="IVY71_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985250.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3516..4457
                     /locus_tag="IVY71_001184"
     CDS             3516..4457
                     /locus_tag="IVY71_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7985251.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4459..5349
                     /locus_tag="IVY71_001185"
     CDS             4459..5349
                     /locus_tag="IVY71_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP7985252.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5392..6192
                     /locus_tag="IVY71_001186"
     CDS             5392..6192
                     /locus_tag="IVY71_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7985253.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6207..7058
                     /locus_tag="IVY71_001187"
     CDS             6207..7058
                     /locus_tag="IVY71_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7985254.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7097..7258
                     /locus_tag="IVY71_001188"
     CDS             7097..7258
                     /locus_tag="IVY71_001188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985255.1"
                     /translation="MLQIIGDRDMDELSQQESRMVRKISKLIIYRDTERFLTVLGNVY
                     ENPELLEVE"
     gene            7259..7720
                     /locus_tag="IVY71_001189"
     CDS             7259..7720
                     /locus_tag="IVY71_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7985256.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7746..8198
                     /locus_tag="IVY71_001190"
     CDS             7746..8198
                     /locus_tag="IVY71_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985257.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8304..8498
                     /locus_tag="IVY71_001191"
     CDS             8304..8498
                     /locus_tag="IVY71_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985258.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8529..8915
                     /locus_tag="IVY71_001192"
     CDS             8529..8915
                     /locus_tag="IVY71_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985259.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8912..9115
                     /locus_tag="IVY71_001193"
     CDS             8912..9115
                     /locus_tag="IVY71_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7985260.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9112..9408
                     /locus_tag="IVY71_001194"
     CDS             9112..9408
                     /locus_tag="IVY71_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985261.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9484..9900
                     /locus_tag="IVY71_001195"
     CDS             9484..9900
                     /locus_tag="IVY71_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7985262.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10708..10938
                     /locus_tag="IVY71_001196"
     CDS             10708..10938
                     /locus_tag="IVY71_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985263.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11097..11930
                     /locus_tag="IVY71_001197"
     CDS             11097..11930
                     /locus_tag="IVY71_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP7985264.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11923..13338
                     /locus_tag="IVY71_001198"
     CDS             11923..13338
                     /locus_tag="IVY71_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAP7985265.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13350..14879
                     /locus_tag="IVY71_001199"
     CDS             13350..14879
                     /locus_tag="IVY71_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7985266.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14971..15849
                     /locus_tag="IVY71_001200"
     CDS             14971..15849
                     /locus_tag="IVY71_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP7985267.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15851..16168
                     /locus_tag="IVY71_001201"
     CDS             15851..16168
                     /locus_tag="IVY71_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985268.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16217..16918
                     /locus_tag="IVY71_001202"
     CDS             16217..16918
                     /locus_tag="IVY71_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP7985269.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            16933..17823
                     /locus_tag="IVY71_001203"
     CDS             16933..17823
                     /locus_tag="IVY71_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP7985270.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17846..18061
                     /locus_tag="IVY71_001204"
     CDS             17846..18061
                     /locus_tag="IVY71_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985271.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18073..18414
                     /locus_tag="IVY71_001205"
     CDS             18073..18414
                     /locus_tag="IVY71_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985272.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18414..18785
                     /locus_tag="IVY71_001206"
     CDS             18414..18785
                     /locus_tag="IVY71_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985273.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18778..19173
                     /locus_tag="IVY71_001207"
     CDS             18778..19173
                     /locus_tag="IVY71_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP7985274.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19175..19552
                     /locus_tag="IVY71_001208"
     CDS             19175..19552
                     /locus_tag="IVY71_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985275.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19553..20161
                     /locus_tag="IVY71_001209"
     CDS             19553..20161
                     /locus_tag="IVY71_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7985276.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20161..20502
                     /locus_tag="IVY71_001210"
     CDS             20161..20502
                     /locus_tag="IVY71_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985277.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20744..>22636
                     /locus_tag="IVY71_001211"
     CDS             20744..>22636
                     /locus_tag="IVY71_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP7985278.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLT"
BASE COUNT         8177 a         3302 c         4663 g         6494 t
ORIGIN      
        1 aatctgatag tgtttcttcc ttacttaata taaatctttc tctatcatgt aatttattta
       61 gtttttcatc atttttttgt tctaattcat aagcttcttt ttttattgtg ttagctttcg
      121 acagtttagt gattcctata attaataaaa taagtccaat caataataac attcaaaact
      181 cctcatttct ttgatataat tattgtagag tttactcaga aatgaggaaa gagtccatgt
      241 gaaagcatgg gcttttttat ttttaatgtt gtaatactaa atgttcaaga ttatgaggaa
      301 gccctaaata ttcaataatt tggaactgac tcatatcaga taaatcttgt tcatctattg
      361 ttctagttaa catttcaatt gcaaacatat tagcttcagc ttctatacca taaatcatac
      421 aactaaggtt gtttgctcta aaagctgctg tgcaaatacc tttatggagt ctaacgtggg
      481 ctatttcatg ccataataca aatcttttca tagaaggtga taggttttga ttaattaata
      541 tcacataaca tcttttattg tgtatcgttt gtcctaatgt tttattccct aaatcagttt
      601 caattaaatc tatatcagaa tatttgatta ttttatatgg attaatcgca tcagatccaa
      661 actctacaaa taatttttct atttcttcta atatccagta tttgtttaaa tgcataccaa
      721 agcccaccta ttgacgatat tttttaggag tgaatttttt ctttgcttta actttggtta
      781 aggcaagtgc aagttgaatg gcatcgtata atttttcctt atcttcatcg tctaaaggtt
      841 caccataata atttgtttca gcatctgaat tcaagccttc aagtaatttt tcagcttgta
      901 aagcaatatc ttgtttatct ttttctgtta aatcataata atggcgttta tcagtacgtc
      961 ctaaaagata atcagttgat acattaaagt aatctgcaac tttttgtatt ttatctccag
     1021 atggagtact tttattccat ctagacatgc ttccatttcc gaaatttaac tttctttcaa
     1081 gttctgccaa cgtcattgaa cgttctttta ataagatttt tatacggtct aatagcatag
     1141 gatatgcatt cctttcaaaa taccgacaaa aaaagtattt ttctgcaaat tatctattta
     1201 caaatagaga atttgcagtt atactagttt tgtaagcaag atatttacgg catacaaaga
     1261 ctaaaagcaa taaactaata gtctccccag acatcagtaa gctcgctttt tgtgcgctgt
     1321 attttgtatg cttacataat agcatatttg cagtttttgt caaacttatt agacaaaaat
     1381 attagaaaat attcgataag ggggtatgaa gcatgacttt aaaacagcaa gtattaattg
     1441 ctttagtaaa gaaaggttgg agtcaaagag agttagctcg acgaatgggt atatcaatta
     1501 cttatttacg agatatcttc atcgaaaaaa gaaaacctaa ggaacgttta aaacaaatcg
     1561 aagaactttt ggatatcaag ttagaagtag attctaatga tcagccatca gaacaggagg
     1621 cgtaaaaatg gtacaacgtt tgagcaaagc ggagtttaaa gagaaatata agtattctga
     1681 aagtacttat caaagaagaa tgaaggaatt taaaaaatca cgttttagtg aggggtataa
     1741 cgcagttact tccaaagaaa taacaattga tgtcgattta tatgaacagt ttagaacatg
     1801 gcgttctaaa aatcgattta gagttagagc tgtttgttaa tagctaaaaa atctaaattc
     1861 accacttctg aaaagaggag ttgctgctct cctttcaagt ttgccaaagt ccacgttttt
     1921 tcattttggc aactcctctt cttggaggtg gtgacggcga gagacgaagg agggatgaca
     1981 tgaaggaaga atcaaaaata gggattttga atgagaacaa ttacacaaga ttaattattg
     2041 aaacagaaga agggaaaaaa gtagcagaag ttacattgac cgatgcaact cctgctgacg
     2101 gttatcgtat tcgattaact cctaaatatg attaaccttt tggtgggtgt ggatcgtttc
     2161 cgtgactatc acggctttga atttttccat ctctgccatg aataaatagc tcagatcctt
     2221 gatttttaga aatctctcta gcgattttta ctgcttctga ttttgtatta gtatgtgcag
     2281 tcgctttaga atttccagca cctttgacat tccaaccacc attggaatta ggtacaacat
     2341 gttgattttt tcccatagtt ttcacctcca tttctataga aatattttat caaatagaaa
     2401 tggagaaatt caataaacga aaggagaagt gatatggcat ataccttgca acaagaacat
     2461 caaattctcg gtttgattaa acaacgcagg aaacaattac aagatgaccg tgcagcgctt
     2521 agaaaagccg atgagctatc agataggcaa gctgaactaa ttgcttctga acttgaggat
     2581 ttgagaatgc tagaaataaa aaatagggag attagattat gaagaagaca gacacacttt
     2641 ttataggatt cattttgggg ttattagtga ttgtagcgca ccaaagtatt atcgggggaa
     2701 gcttgttcgc agcattgatg gttttaatca atctgcttga ttcaaaagaa aggagcaact
     2761 atggcacgag aagaagcgct aaaaatcggt aaagtgattg ctgataatcg gtggggaaat
     2821 agccgcccta ttattttaag caagcaacat atcgaaaagc aaaaagcatg gcaacaaata
     2881 aaaaagtgac tctgccagcc agcaaagagt cacatacaaa tttataaaac taggagaatt
     2941 atatcacatg aataagaaaa ttgaaaatct tatcgaagaa ttgaaacgtg aatgccaaaa
     3001 gcaaggtgtt tctattattt gtacagccca aaaagaaggg gaacttaaaa gtctcgttta
     3061 tggtgagaca acagaaattt tactttgtct tgctatgcaa gaagaacatt tagatgaaaa
     3121 tctcccattg tcagcacata ttatgcgtag aattgctgtg gatgcttacg aacaagcgaa
     3181 aaatgaagaa gaaaatcaac cttcaaatca tacttttgtt attaataaca aggaagattt
     3241 agctgatgtg atgactcgta ttctcaaggg ggaatttcaa tgatgaatga aaaaacaatt
     3301 gatctactca ttattttatt tttcacgtta gtgattgtag cgaaaagtta tgtaatcttt
     3361 ggcttgttat tcgcaattac catgcttgtg ctttgcgtgt tagcaagcag aaaagaggtt
     3421 aaacatgaac gagttagcaa atcttgacaa ctatttaact gatcctgatt atgctaaacc
     3481 accatatgaa gcaccaattg atgaggagga tgaagatgag taaatctacc ttagaaatga
     3541 gccatcaaga atggcttgaa gaccgtaaga aaggcatcgg aggttcggat gttggaacaa
     3601 ttttaggatt gaataaatgg aaatctcctt atcaactatg gctagaaaaa acaggacaag
     3661 tagtacttga agaatcagga agtgagcccg cttattgggg taatgtttta gaagaagtgg
     3721 ttgctaaaga gtttcaggaa cgtacaggca aaaaagtacg cagaagaaac caagtatttg
     3781 aacatccatt acatccattt ttaagagcaa atattgatcg ggaagtagtg ggagaaaatg
     3841 ccattctgga atgcaaaaca gctaatcaat ttctcggtaa agaatgggaa ggcgaagagg
     3901 taccgcttag ctatctctgt caagttcaac attacatgaa cgttctaaac aaagactatt
     3961 gttacatcgc tgtcttaatc ggtggtcaaa aatttatctg gaagcggatt gaacgagatc
     4021 aagagctgat cgatacaatc actgaacaat tagtagagtt ttgggaaaca aacgttcttg
     4081 gaggtatcga gccaattatt gatggtagtc aagcgacagc tgatttcttg aaagagaagt
     4141 atgcagatgt tgaagacgtt caaacgacgt taccaattca tttcgatgaa ctagttgaac
     4201 agaaaaacga actcaaacgg actaagaaag aaattgaatc agctatccgt caagtagaca
     4261 acgagattat cagcgagcta ggtaaacgtg aagccagtat cggtatcact caaaaaaaca
     4321 tcatcagttg gaaatttgtc cgtacgagac gtatgaactc gaagaaacta gcagagaaat
     4381 atccagatgt cgcaaatgat gaagagattt ataacgttac tgaatcaaga aggctaaccg
     4441 aaagggagat caaataatat ggcaacaaat gaatcgttaa aaaatcaatt ggcagcaaag
     4501 ccacagaaac aagttgcacc aggtcaatta ggacttaaag ctttgatgaa tacaccaaca
     4561 atgagaaaga agttcgaaga ggtgcttcat gacaatgcta atgcttttat gtcgaatgtt
     4621 atgactcttg tatctaatga cagttatctt gcagatagtg aaccgatgtc tatcatgagt
     4681 ggtgcgttaa ctgctgcaac attaaatctt gggctagata agaatttagg ttatgcatat
     4741 ttagttccat tcaatagtaa aaacaagcaa acaggaaaat gggaaaagaa agctcaattc
     4801 atgcttggct ataaaggata tatccaatta gcccaacgat caggtaaata caaagcatta
     4861 aatgtgatcg aagtttacga aggagaacta aaaagctgga accgactgac agaagagttt
     4921 gagtttgatc caaatggtag aacgtctgat gaagtcattg gatatgttgg ctatttcgag
     4981 ttactgaatg gattcaaaaa aactgtctat tggaccaaac aagaaatcga agctcatcgg
     5041 attgctaaca ataaagatcg cgataagaca aagttaagtg gtgtgtgggc atctgattac
     5101 aatgcaatgg cacgaaaaac tgttttgaga aatcttcttt ctaaatgggg aatcttgtcc
     5161 atcgaaatgc aagaagccac cacatcggat gagagagtcc aaagggttca agaagacggc
     5221 agcattattg ctgaaacaga agttgaagaa gatattcctg aaagaaaaga agcagaggtt
     5281 atatctgaag aaaatgaaga tgtacaaact ggattatttg atgcatctaa tccgccgtta
     5341 aacaaataat gagggagctt tctcccttgc ttttctagag gagaaatacg aatgaacaca
     5401 ggatatgtca aattacatcg aaaagtgatg aattcattcg tttggaccaa tccttatatg
     5461 tataaattat ggaacttgtg tttgatgaaa gcgagtcatg agaatcgcaa gatacttttt
     5521 aatggaaaag agatacaagt gaacagcgga gaattcgtca cagggcgcga tgctatcaca
     5581 tctgagatga acgaaggcgt gaaacgtgaa catcaagtga acagcggttc tgtatggaga
     5641 tggctaaaac aatttgaaaa aaacggaatg ttgaacatca aatcaactac gaaatacagc
     5701 gttatatcaa taaaaaactg gtctttgtac caaagcagtg aacaacaaat gaacatcaaa
     5761 cgcacaacaa gtgaacaaca agtgcacaca aacaagaatg aaaagaatta taagaatgaa
     5821 aagaataata aaacatcatc gtcgcaacca cgcaaaaagc gtgtttacga caccgactca
     5881 gtttactaca ttctcgcgga ggagttattc aaacagattt gtcagaatca ggaaatcaaa
     5941 aagccgaatc tgcaaagttg ggcagataac attcggaaaa tgatcgagat cgacaagcgg
     6001 accgaaaacc aggtacgggg aatgattgaa tggagtcagc acaatgtgtt ttgggcatcg
     6061 aatattttat ctgctaaaaa attgcgagaa aaatacgaca caatggcagc gcaagccaat
     6121 cgtgattata aaacaaaaca aactaaaacg cttgaatacg agaaatttag cacagatgag
     6181 ttgcctattt gagaggaggc gtaggcatgg aaactgttgg ggaaatcatg gaaaagctga
     6241 tacagaaagt gcttgtccaa cgtggcgaat gtcctgaatg cggacagcct ttgtatggat
     6301 ggcgtacgaa gaaccctgat ggctcagaac gttgtaagcc aacctgtatg caatgtgggt
     6361 ataaagcatt gcgagttcag gaagacttac aaacagaacg aatttacaac gagagcctga
     6421 aagcaagagc aatcaatttt ttcaaaggtg gttctgttgt gcctaatcaa gcgttgtttg
     6481 attgcacatt gcagaattat caaattgtcg atcaagaaac aagacaagcg gttgaagtaa
     6541 ccaaacgctt tgttaattca gtcttgttag gaaatccaag tcaccttgtt ttaactggaa
     6601 aacaaggaac aggtaaaagt cacctagcga tggcagcggc ttgggaagtc ttgaagcgat
     6661 caaactacga taaaaaaatc ttatttatcg ggttacagga aatgctggat caaatcaaat
     6721 tttcgtacaa caatcctgaa ctcagaaaaa cgattgaggg atcgttgatt gcagatatca
     6781 aaacagcgga tttagtcatc attgatgata tcggttcaga actaggaaaa gatgcatcag
     6841 atagtcgagc gtttggcata aacacgctaa attcgttctt ggatgcacga cagaacctag
     6901 caacgattat cacaacgaac ttgcttggtg aagaactgaa aaaagcttac ggtacgagaa
     6961 caatatcaag aatgtttgtc aactctgatg gatttacgat ggtattttct caaacagcag
     7021 acaagcgcat aaaaccagtc aaaggagcga tcgcatgaat ttgactaaac aagaaaaaag
     7081 aatctgtcgt cagataatgt tgcaaattat aggtgatcgt gacatggacg aattatctca
     7141 acaagagagt cgaatggttc ggaagataag taagctaatt atttatcggg atacagaaag
     7201 atttctcact gttttaggta acgtctacga gaatccagag ttattggagg tagagtaatt
     7261 gccaaaaata ttagatgcat gttgcggtag tagattgttc tggtttgata agaacaattc
     7321 tgacgtaaca tttatggatt gtcgtcagca gtatgaagaa ttatcaacag gtcatgtgat
     7381 caatgttgat ccagatgtgg tcgcagactt tcgcgatatg ccttttgaag atgatgtgtt
     7441 cgatatggtt gtatttgacc cgccacactt aagacatgtc ggcgaaaact catggcttgc
     7501 caaaaaatat ggaaaattag acgaactttg gccagaagac atcaggcaag gttttgcaga
     7561 gtgtatgcga gttttacgac cttcaggaac attaattttt aagtggaacg aggaacagat
     7621 accactttct gatgtgttag aggctattgg agaacagcca ttgtttggta ataaacgaag
     7681 caaaacacat tggcttgtgt ttatgaaaaa attcagctaa cggaggaaac aaaggaggaa
     7741 aagtaatgaa gattgtatta aataaatgtt ttggtggctt tgggttgagc catgaagcaa
     7801 aaatggaaat ttttaaaaga aaaaatattg aagtattccc atacatcaat aactttagct
     7861 atgactcaga tgatgaatac acgagataca caggccaaaa gctaggttcg atggatttta
     7921 tctactattt caaaaaagac ccgaaaatcg acaaagttac aggaatttat agcgaaattg
     7981 accgactgta cggaattgct gatgattcca gtttttcaag tgattcaaac agaggtgata
     8041 aggacctcgt tgctgttgtc gaaaaactag gaagtgaggc cagtggacca tatgcaagtc
     8101 ttaaagtcgt tgatataccc gatggggctg agtgggaaat aagtgattat gacggcgttg
     8161 aaactgcaca ttatggtttt caaacaggta gtatttagtc cactaatgac aactaaagag
     8221 gaggcagaag gatgagtgaa gatgaatact ttgaacagta gtctattaac gacagagatt
     8281 tttaactgga aaggggtgta gtcatggacg catcggaagt agtaaataaa attaatagtc
     8341 tcaaagaggt tttcggagac gtcgaagtag tgataaacgc acaaggctat gacaacggat
     8401 tttttaagca gattaccgac attagcattc agcaaggaat ggaagacgaa gatggaaact
     8461 ttatcgatga agtagccatt ttagtgactt gcgagtaagt aattgacagc taaagaggag
     8521 gcagaaggat gaaaaaattg caccaactca tatctgagaa agaaagcgaa ctccaaaatt
     8581 tagaagatag tttgggattg gggtttccaa ttgttgagca agctaaaatg actcaaatca
     8641 gtcatctacg acttgaattg gaagatttaa gacaaataga aaaatcaatt caactcaacg
     8701 acaaccagaa aatcgtgttg gagtggttga aaagcgagac aattttaact agagaagcac
     8761 caatattatc tgttaatgct ttttctgata aaaatttact aggaaaatta cctgataaag
     8821 tacgcaaagc ttataaacta ttggcttgta aacaagaata tgaagtccta tcagcattcg
     8881 ctcaatgggg attagaacag gaggaagcgg aatgaacaac agacatcgca gaataacaaa
     8941 actaagaaaa caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc
     9001 agcagaagaa acatataaag tggcaagtca gtttgttgct gatgcgagta atgctattcg
     9061 taagtttggg atttcgatat taaatgatga tcgtaaatgg gaggaaatga gatgaaaata
     9121 aaagacggat tttacgctag tagtcatggt atcggcggtt taatgctaga catgccgata
     9181 aagaacccta aaacacgtca gaaatcaaaa ttcaaaatcg gtgacatggt ccgatgtaaa
     9241 gcagaagagt tcatctatcc gtttcgtgga tatgtagagc atgtctataa tcattcagca
     9301 atcattcaca ttgaaaacac gatggaatgc gataagtggt tagcgaaaag caaagataat
     9361 ttagcagtgg ctcgtttggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc
     9421 gctccgactg attcaataaa tccaacacat ttattatatc acataaagga gcggtttgac
     9481 ttgatgcaat tgttacgaga ggtagatttc aaacagacaa gatgtaatgc gagagatgtg
     9541 ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtcg
     9601 ccgattatta cggatatgcc gaaggcaccg aagcacggca ataaggcaga agacgcgatc
     9661 attcagatga tggatataga agcggagaga gacgcaatac tagcggcttt gatggctctt
     9721 agtctgatta gccgtcagat actctactac agcttctgtg acgtaaacaa gcactctaat
     9781 tatgaaatag ggcaattgat acgaggatac ggagagaaga atgtagagaa gttgaaatcc
     9841 atcgcattaa tcgaatttgc agaagcatac aaaaaaggcg tgttagttca gtatcgttga
     9901 ttttgtaggg tttttgtagg gatagtgtag ggtttttgag cggtttaacg tgatattatg
     9961 gtagtgtcga aagattagtg ataggtctga gacaaaataa aatgtaaggg aggaaatctc
    10021 cctcatcgtt ttaacttaag cttcgataga cagcaacgga aatattaaga ataaggatgt
    10081 gaatttcaac tccttctaaa ttgttcttat tatctatcat ccgttgttgt ctattaattt
    10141 atgcatcgga ggaaaaacga atggataaag aaatcaaagt aactgtcaaa ttagatttga
    10201 ctgaactaaa agaactgctc aacaaggcta gtgaccaagt cgaacagtta caagaaactt
    10261 tagatgaaat tgctaatttt aaaatccaag tttcttagca gtatattttt tagcagctgc
    10321 actcataaaa tcataccaag tatcagaaaa atataactta actaaataat taaaaaaggt
    10381 gggtaaatag aaatgacggc gttaataatt tcaatttttg cgctctgtct taatgtctat
    10441 atgattggat ttaaaaatgg acaaaataaa aaatagtagc agccgagaat aattttatag
    10501 tgtcactgtg gcggaaatag aagacgcaaa ggtggaggta ggaagcgtat ggcgcactat
    10561 cgggaccttg tacaaaacta gaaaggacta gtacgtgtgt ggtgcgagcc ctagggagga
    10621 acaggataac cgcctgtgtg taggttgcta ttacatatta gatcactctt tgagtggtct
    10681 ttttatttac ataaaggagg ctacataatg agaaactact ggtatatatc gctaactaat
    10741 gaatatcctc gaaccattga tgattgttca gtgcgtgttg tgcgttctgt ccaaatcaaa
    10801 aagaagtact ccattgttga aatgaccaga gaagctacgc caaaagagat tgataaatgc
    10861 aaacttcttt attgcggtca tggtttctat gatgaaccaa acattcaaaa taatattaac
    10921 aagaatttga gggattagaa tgcaagaaat ggcttaaaaa tctccaatca ggacaaatca
    10981 agttagacaa agtttccgat ttaaagatat tgattgaagc tgatttaatg ctaaaagata
    11041 ttgaaaatta gaaaacaaaa ctcaacctaa gaagattgcg aggtggtgtg tattgaatgg
    11101 caagacaacg tgatccaaga cgtgatgaag ccaaaagaat ttggttagaa tccaacggag
    11161 aaaagcagtt aaaggaaatt gcatctgaat taaatgtttc agattctcag gttagaaaat
    11221 ggaaatcgca agacaaatgg agcgctgaat tgaaaagtaa cgttaccaat ggcaaaagta
    11281 acgttactaa tcaaggtggc gctcctattg gtaatcaaaa tgctaaaggt aacaaaggaa
    11341 acagcagagc atcaccgcca aagagaaata agaatgctct taaaactggt gagtatgaaa
    11401 caatattttt tgacacgtta accgataatg agaaggacat ctattctagt ttgaatgatg
    11461 atccttcttt tgctttgtcc gaagaaatac gacttcttaa aataagacaa cttcgaatga
    11521 tgaaaagaat ccaacaagct gaagctggac taaatgatga agaagtcgaa cgattgcagc
    11581 agctaagaaa gattaaaact ccgattgaaa aaaatggtaa aaagctagaa atcaagcgtg
    11641 aggttatgca agatgtgcag attagcagaa aaaaacatcg caaaattgat gatattcttt
    11701 caattgaaga ttcattgact cggattagca accagttagc taaagccatc aagcaaatga
    11761 atgaacttta tatgaatgaa tacagaactg atttaattaa agctcagact gataagatcc
    11821 aagctgagac aaatgaaatt ggcggaaata attcaggtga agaaatagaa gaatggaaac
    11881 aggcagtttt aaatgccgca aacaaacggg cggtgaaaga aaatgaataa tgaatttatt
    11941 ccttttgctg atattggttc tgccattgat tattactatg ataaaccagt agctttttgc
    12001 caagatattt tgcatttgaa tcctgatgaa tggcaagaaa atgttttaaa tgatttagct
    12061 gaattttcaa aggtttctgt tcgttctggt caaggagttg gaaaaacagc attagaagca
    12121 ggagcaatac tttggttctt aacgtgtcga ccctacgcta aagtaatagc aacagctccg
    12181 acaatgaagc aactttacga tgtactttgg gcagaggtag ctaaatggtt aaatgatagc
    12241 ttgatcaaaa acttactgaa gtggacaaag accaaaattt atatggttgg tgattcagag
    12301 cgttggtttg ctacggctag aacagcgact aaaccagaaa atatgcaagg ttttcacgag
    12361 gaccatatgt tgattgtggt agatgaagct tctggtgtgt ctgatccaat tatggaagct
    12421 attcttggta cgctatcagg ttttgataat aagctgttga tgtgtggaaa ccctaataat
    12481 attgaaggtg tcttttacga ttcccacaat tcagaccgtg ataaatacag agttcataaa
    12541 gtatcaagct atgatagtaa acgtacaaac aaagacaata tagaaatgat tcttaaaaaa
    12601 tatggaaaag aaagtgatgt tgctcgtgtc cgtatttttg gagaatttcc caaaggtgcg
    12661 ttggattcat ttatcagtct tgaaacggtt gaattggcta cagaaaaaca aattagtgat
    12721 tctttagtca ataaaacaac ggttgctcat attggtgttg acgtagctcg atatggtgat
    12781 gattctacga ttctctttcc tagaattgct accagggcat tggagtatga gaagtattca
    12841 aaacgtagca ctatggaaac aacaggatat gtcatcaaca tggccaagaa tctaatgagt
    12901 caatatccga gtattgataa agtgatgatt aaagtcgatg acactggtgt cggaggtggt
    12961 gtaaccgacc gcctagaaga acttatagaa gacaagcatt atccttttga ggtgtttgga
    13021 gtgaataacg gttcaacatc agaagacgat ttttacgata atttaggtac tcaactatgg
    13081 ggaaacatca aggaaatgtt agaagaaaat atgacagcaa atcttaacgg agaacagcct
    13141 gttattgaat tgccttctga tagttcgtta atcaaagaat taagtactcg caaattcaaa
    13201 atgacaagta gaagtcgtat acgtttagaa agtaaagatg atatgaaaaa gcgaaatatt
    13261 ggtagtcccg atattgctga cgcactggct ttagcgtttt atgagccacc aagtcactat
    13321 caatttattc aattttagga ggtgagtttt tgattagtac agtattatct ttagagagat
    13381 ataagaagct acgtgttaaa tatgcaacac aaattgaaga tggaatgttt gatccgaatg
    13441 gttttataga atatatgaaa ccattttttg ctgatcgcga aagaaaatat ctagcttata
    13501 ctagcgaaaa gaatgaaata gacaatagac caaagcctaa cactgatatt gtaaaagtta
    13561 ataataaact tcatgctggt atgtattcaa ttgtcgtaga ccaagcagtc aatcatttta
    13621 ctggtatacc tatcaaatgg gattacgatg tatcggaaca aaaaagaaca ctcattcaaa
    13681 gattaaaaga taaattctta aaaaatgata tagaacttcc aacagttcca gaagctttta
    13741 acaaattaac aagcaatctt gattccatga gattcgcaat gcttgattct gaaactgcaa
    13801 cttttcaagg agcttgtgga gtagctttcc gattgttaga gcctgtggaa gaagatgatg
    13861 gttggaagtt aagagcaagt aatattgaac cttggagagc agaaagatat ggaaatgccg
    13921 gaatctatat caaagaaaag tatgactctt accagaaaaa attttttcaa gaaatgaaag
    13981 ttatgacaag aaataaaatt ctgacatatg cttgctatgg tgatttgaat tttgttacaa
    14041 gcggaacgtt caaaaaaatt gatgagaccg ataacccttt ggggacgatt accttggcag
    14101 aatttaaaaa taatacgaat cgttattgtg attttgaagt agctgaggaa attggagatg
    14161 caattgatcg tgctttgtct gatcaacaaa acgaaatcga acaatttaag cttgcgtaca
    14221 tgcttgttac tggcacaaca atgagcaaag gcactgctaa agaaatgatg aatcagctag
    14281 ggattatcaa cttaaaagac cctactgcaa aagctgaata tgtcacaaaa aaattagcaa
    14341 aagatttcaa tgaatatcat atggatctat tgaaaaaaca gttttacacc atttgtaaag
    14401 caatcgattt taacgatgag gtgtttaaat caaacagttc tggagaagct cgcaagtggc
    14461 aaatcattag cctagaagcg aaaacaaata ctaaagagca atattttaga gaaggattga
    14521 aagaatgcgc agaaacaatt gctgcttttc ttaagtttca tgacaaagta gaaattgagc
    14581 ctgaaaaaat tatttttact ttctctagat ctttgccaac tgatttaagt tatttagctg
    14641 aagctttacc taaacttgca ccatacgtat ccaaacgaac tattcagagc caaattccat
    14701 ttgtaacaga tgttgattat gaaaatgaaa tgatggaatt ggaaagtgga agtgcttatc
    14761 cagatagtga atacaatttt ggcggaggtg gcaatagtga cgatagaaac gaagtattgg
    14821 accaaacgtc gagaactgga ggatcaagca aggctcaaac aagaaaatca gacacttaaa
    14881 aaattaacta gtgtatttcc tgaagcactg aaagagatac aagcaaaact attatcacaa
    14941 gctgacttac acaatatcac ttatccagaa atgatggagt tttacagtac aagtaatcag
    15001 aaaaaatatc gtgaatatgt ggaaaaaaat tataagtcat taaaaatgta tgatgcaaaa
    15061 tacaaagagt ttatcgatga attttttcca ccatttgact atgcaaaagt caatcgctta
    15121 ttacaaatac gatcagatgt atttaaaatt cttgcagaat atgcgatgga tgcagatgtg
    15181 aatcaatatt tttctgatcg cttagaggaa attcttcaaa gaacatattc ttctaatgct
    15241 aatgtttttg ttcaactttt aaacgttgat ataccaaatt atttaccaga aaatgaaatt
    15301 aaacattatt tgaactatcc atggtcagga aagacatttt caagaagact ttggggaaat
    15361 atttcatcgc ttgaacagaa actctctaat gctattgtaa aaagtgttgc tagtggagaa
    15421 ggcgttatac acgcattaaa caccatgaga ttagactcag aaatttgtga catgtttaag
    15481 ttagaagaat caaagtataa caaagcgata gaaaatctcg ttcgaacgga gtatgcaaaa
    15541 tttgcacaag atggtattga aaaatcatat ttagaaacag gtattgagga atacaacgta
    15601 ttgactgcaa aagatgagag agtttgccgg atttgtggag gaaaggcaag taagaatccc
    15661 tataaactga aagatgctgt cataggtgaa aatcgagcac ctttccatag tcgttgtaga
    15721 tgtacggatg ttcctaattt accaaaatta ggaaaggata ttgatgaaga atatgaccgt
    15781 ttatttggcg atttattaga tgagtttgca catgattctt ttggaattaa tttgaaacgg
    15841 aggaagtaga atgaaagatt tttttgaagc agtactaaca attaatgtaa atgctgatat
    15901 tgcagaagcc tacaaaacag ctattgagtc tgagaaccat cctaatggct tgagagacca
    15961 ttggaatggc aattatgcct acgtggttat tggcgatcaa actgttaatt atcaagataa
    16021 tactccagtt gataagaata ccgttaattt aacgattcaa ttattatctc attcattacc
    16081 aaatttaaaa gaaacagttg attggtatga aaagatggga tgtattgttg ttagaactga
    16141 ctacaaagaa ggaaagtcta gtaattaggc ttttttattt tgtccgaaat gacgttaaac
    16201 tagcgcaata ctgggcttgg ttgaatggtg gggcgcaact attaaaactc aaagcaatgc
    16261 ggggcgtgaa aacgaatcgt ggggcgaaag gagaatgagc atgaaacaca aatcattaat
    16321 gccaatgaat ttgcaatact ttgccgaagg tgacgatcaa aagttttctt ttgatgactt
    16381 caaatctttt gtggaatcca atgaagaagc tcaaaaattt attcagtcac aatctcaaag
    16441 tgttgccgac aaacaattgg aagcttggaa acaaaataac ttagataaaa ttaaacagga
    16501 taccatcaag gaatatgagg aatctaagaa aaacaagtca cctgagcaaa ttcaactgga
    16561 aaaactacaa gctgaatttg aagcagaaaa agcgttgcgt gtgacaagtg ataataaagc
    16621 atttgttgca gaacaaattg ctggattaga actagatgga gagttaaaag agtctatttc
    16681 tcaatttatg ctaaataatc ttgttagttc ggatacagat ttcactaaga atgctgttga
    16741 aggttttaca agtgttttga atgcaattaa tgagaaacat gcagatgcat taaaagaact
    16801 acaaatgaag tctgcatttg gtggaactca acaatcgaat aaccaggttc agcagaacaa
    16861 tgaaacatta acaaatccag aagaacaatt aggacaaatt cttcaacaat ttaactagga
    16921 gagtgaaaaa ttatgaaaaa aacatcttta aataatttag agtatttgga tatttcacca
    16981 gcgattaatg ctatgcaagt accaaataca ccttttttaa gctatttact tggtgctgga
    17041 aaaacagagc cagcaaactc gacagaaatt aaatggcgtg aatatgatat caacaacgat
    17101 gattcttctg aaaaacttga gggcggagaa tatccagatg ctgaatcagg tcgaacttgg
    17161 tttaacaact atactgaaat ttttagaaaa tcaacctctg tatctggtac attagatgct
    17221 attaatgtga atggagtcgg aaatgaatta actaatcaag tagcactacg tggtatggaa
    17281 atgaaaattg atttgaaccg aaaattgatt actggtgtaa aagctgatga aaatggttct
    17341 aaaggtcgtc gaatgaatgg gattttgaac ttgatcaatt cagcaaataa ggcagaaaca
    17401 gccactgcgg gtgcagtaac aagaaaagat atcgatactt tatttaaatt gatgtatgaa
    17461 aaaggttata tgggagaaaa actatgcttg atttctccag atatgcagga gttaatgact
    17521 gatgagttag atggaaaatc aacaaaaatt gttcagttcg gggaaagagt aacttttgga
    17581 ttgcaaattg gaaatatcgt gtctaattac ggtacaggta ttgctctact agaaccatca
    17641 ttgccaaaag gaacaattgc cgcaatcgat actaattatg tgaaactacg tccattacgt
    17701 gaatggagag cagaagaact tgcaaaaaca acggattcta gacgtatcgg tcttgtaggt
    17761 gaatactctc ttgaatacaa tgcttcaaac tctggggcaa ttttaaattt aaagtctgaa
    17821 taaaagggag ttagtactcc cttttttgat aggaggaatt acagtggtaa aaaaagatga
    17881 aactaaaaaa gatgaagtcg tgaaatatag agtaggtaaa actaaaaatt ttgttgggtt
    17941 tgttcatcct aaaactcgta gatttatcac agcagattca aataacgaat ttatcatttc
    18001 tatagatgat aaagaagcaa ttgcaatttt ggaagatgca attgatgtta atgaaattta
    18061 ggaagtgatc tgatggatga atcgctaaaa acggaaatca ttgagtctac aaaagaagat
    18121 tttccagatt tgagtgaaga acgcataact aatttattag aaataatttt gctagaaatt
    18181 gaatcataca acacttgtaa aaatgatatt tcatgggaaa agttaaaaag cgtgattagt
    18241 gaagtgttgt ataaaataat aaaaaatgaa tcagaaaaaa cagtatcttc agttagacgt
    18301 ggcgatacaa cgattagtta tgcttcaaca acaaatgatg ttagtgagtt acttctaggc
    18361 tatggtgatt tgatacgaag agttattggt tgtggaggat tggagttttt ttaatgaatg
    18421 aagcagatat tttagaaact acttatgaag atagttgtat tattgaaaga ctgacggaca
    18481 ttgaagattc taatacaaat attactattc aagattacaa aaaagtatat gataatccta
    18541 tttcttgcgc tctttcacaa ggtcaaatcg atggactagc agtcatagaa gatggagaga
    18601 tggtaaatgt ttcaactgac acatataaat tatttgttca tcctaagatt aaactaaaaa
    18661 aaggagatcg aataacaata actcaaaagg ccagtggctt aattttttct ctatttgcta
    18721 ctaagccttt ttactatcct agtcattgtg aagtgaattt gataggaagt gagaaaaatg
    18781 ggtaatctca aatatgaatc aaatgcagaa aagatcattg agaattttaa aaatatgact
    18841 gtaattgctc aaaaagaagg aatatccttc gttaacgatt caatgaataa agtcgttagt
    18901 ttagctaaac cattaactcc tgtaaaatca ggtaatttaa ggcgaggata tcgtgtagta
    18961 aaagctagaa aactatcaag cggtcgaatt gttggagcag ttataaacaa tgaacattat
    19021 tttaaatatg tagaagaagg tcataggact aaaaatggtg gctttgtcaa agggaaattc
    19081 atgttaactc gtgcaacgaa tcttgcaaat atgtcttata ttcctcgaag atttaaacaa
    19141 atggcaataa aaatcgttaa gaaaggaaag taacatgaaa gatgaaatca ttgctgcaat
    19201 cagtagcaaa ttaaaagaaa tctatcctga tgggacaata tatcttgatt cagttatgca
    19261 gtcaactaaa gatttttatt tcgttttatc agtaatggaa tctggaactg aaaatgtagg
    19321 aattgatatt caaaatgttt ctttcttaat tgatattgca ttgattgata ataaacctaa
    19381 tagaaattta attaatgaat tagtctcacg ttgtgggact ttttttaata cgattacaat
    19441 cgatgagcaa atattatttc caaaagccta tttacctgat gaagcagatg gtgttcaaca
    19501 tattcgtttt acattagaat ttccacaata tattgaatgg agtgaaagat aaatgggaga
    19561 aaaaagaagt aaaaccggaa ttatttctgt cgaaaaacct acttggtttc ctttggaaga
    19621 tgagacaggg aattttccgg tatatggtac tgcgttgcca atgggaacag cagtaagtat
    19681 caaaccaaca gctaattatg aaacaacaca agattatggg gattcagtgg ttcaagatca
    19741 atttacagct tttggtggtg cagaagttga actggaagca aatggatata ctcataaagt
    19801 tttatctaca attactggag ggaaaattgt taaaggtggt gccttgcgtt ctggagaaga
    19861 tattgcgcaa gatggtgcgt ttgcatatag acgtaaaaaa tcgaatggga aataccgtta
    19921 tacggtcttt tataaaggac agtttgctct agattcagat gaaacatcaa cgattgaagg
    19981 aagtaaagta agttttactc atccaacttg gaagggttca tttgtagatg taccaggact
    20041 tggatatatg tattcagttg atgaagatga tgaaggcgtg gataaagcaa tgatcggaaa
    20101 ttggtttaca aaagtagcaa ttccaattga agaagcagaa ctttcaggag gtacagaata
    20161 atgtcaaaat atcaaacaac gattaaatta acgaaaaaag atgaagaagg taaatatgag
    20221 caagtacaat ttaaatctgc tgaatttcta ccaggcccag tcgtagaaga agcagcagaa
    20281 gtaatggagg gaatgcaaaa tgctgtagat ggaaaatcag tatcagaagc attaagtcga
    20341 gcttattcat ttattgcaga tattttgttt gaaggacagt ttactggtga agattatcgt
    20401 caagggatcg atgctcgaga aattgcttcg ttgacaggta agttattaaa atctgttact
    20461 gcaggctttg atgaaactta tacagaaacg aaaaaaaagt aagcgaagct ctcaaatatc
    20521 ctttttttaa acattcgatt acttatcgag aattagattt aaagacgcaa ttactcgaag
    20581 caggttggac gttaccagaa attgaacgta ctgactttga cgagttaatg cgtctttttg
    20641 cttttcgaga tgcagtaaaa gagcatgagg atgttgagta ctacgataac ttcacccaat
    20701 tttaggaggt gatactttga ataacgatga cctaattctg aagatgatac tagatgaatc
    20761 tggttttact gccggtatga ataatgctgt caaaaagttg aactcttttg atgaaacaat
    20821 cgaaagaaaa agtagaaata gtggcagttc attaggtagt atttggaaaa tatttgctgg
    20881 aagttttcta gctagcggag taactagaat cgtaggagct ggctttgatc taatcaaggg
    20941 ttccataagt ggagcggttg atcgagtaga tacgatgaat aacgctctac gaaatttcca
    21001 aaatatggga tttagtaatt cagaaattat gaagaatatc ggaaagaatg ggcttttatc
    21061 tcaaggtatt caaggacttc ctactgcttt gaatgatgcg ataagtcatg ttcaacttct
    21121 tgcttcttct acaggtgatt taactcgttc aactcaaata tttaaagctt taaatgatgg
    21181 aattcttggt ttcggtggtt caactgatca agttaatgaa gctgttattc aattatctca
    21241 aagtttctca aacggaaaag tagacgcaca aacttggaac tcaatgatca acgctcaact
    21301 tggacctact ctttctgcta ttgctaaaaa gatgggaatt acaatgggag atctgaaaga
    21361 aggtttatct caaggtaaga tttctgttga agaattccaa aatcaattaa tagaaatgga
    21421 taccaaaggt ggcggaggac ttaaatcatt aagtcaaatc gctaaagatt ctactaaggg
    21481 aattaaaacc tctatacaaa atgcaaaaac agctgttaca cgtggtgttg gtgaggtaat
    21541 agaaggatta aataaagcat tagtagattc agatttaggt ggatttaagg gaattattga
    21601 taaagtgggc agttcaatgg aatcgttctt aaaagtaatt gctgcaaata ttcctacagc
    21661 agtatcattt ttaagtaatt tatttaacga aattcaaaaa tttggttctg ctttgaaatt
    21721 catgatgccc tttcttgttc ctgcagctac cgcttttgga gcgttcatgt ttcaacttaa
    21781 aggtatacca gcaataataa aaagcttcaa taattttaag aatgccataa ttggcgttgg
    21841 aaattcatta aagattatgg gggcaatagc tgctgctaat tcatttgttt taattattgg
    21901 tgcagtcgtt ggtgcaatag ctgttttcgg ttactttatg gcaaccaatg aagagcttag
    21961 aaacaaggtt atatctattt ggaatgatgt gaaagattcc gtaattggtg tattaaaaaa
    22021 tataaaggac tggggaattg atacttggaa ttctgctaaa gaaatggcat caaatgcagt
    22081 tgagggtgtc aaagatgctt ggtcaggaac aaaggaatgg ttttctaaca cctggaaagg
    22141 tattaaggat ggggccacag gtttatttga taaaacagta gagacttcaa gaaatgcagt
    22201 cgatagtgta aaaaatgcat ggtctaatat gaagaaatgg ttttctgata cttggcaaag
    22261 tataaaagat tcagccagag aaaaatggaa tggaatcaaa ggttctatta tgtcagttgc
    22321 tggtccattg attacaggta taaagaatgc atttttacat gttacttttt atctagagac
    22381 cttatggaat aatttagttg agattggtaa aaacgtcttt gagattttaa aaaatgttat
    22441 acttgcgccg gttttgttta ttacctccat gatttctggt ggatgggaag aaacaaaaaa
    22501 taatatgatt gctgtttgga ataatattaa agaaagtgcc ataaatattt gggaatctat
    22561 aaagaatata tttgtaagtt attttacaaa tatttatttt gctgcactca atatttggac
    22621 agggttcaag cttaca
//
LOCUS       DACCXW010000038        22174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCXW010000038 DACCXW010000000
VERSION     DACCXW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.038"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            240..2075
                     /gene="tdc"
                     /locus_tag="IVY71_001212"
     CDS             240..2075
                     /gene="tdc"
                     /locus_tag="IVY71_001212"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7985279.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2380..3858
                     /locus_tag="IVY71_001213"
     CDS             2380..3858
                     /locus_tag="IVY71_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7985280.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3824..5155
                     /locus_tag="IVY71_001214"
     CDS             3824..5155
                     /locus_tag="IVY71_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7985281.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5269..7821
                     /locus_tag="IVY71_001215"
     CDS             5269..7821
                     /locus_tag="IVY71_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP7985282.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7828..9267)
                     /locus_tag="IVY71_001216"
     CDS             complement(7828..9267)
                     /locus_tag="IVY71_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7985283.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9242..9424
                     /locus_tag="IVY71_001217"
     CDS             9242..9424
                     /locus_tag="IVY71_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985284.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9613..9975
                     /locus_tag="IVY71_001218"
     CDS             9613..9975
                     /locus_tag="IVY71_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985285.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10162..11019)
                     /locus_tag="IVY71_001219"
     CDS             complement(10162..11019)
                     /locus_tag="IVY71_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7985286.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11139..12050
                     /locus_tag="IVY71_001220"
     CDS             11139..12050
                     /locus_tag="IVY71_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7985287.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12057..13708
                     /gene="mdcA"
                     /locus_tag="IVY71_001221"
                     /pseudo
     CDS             12057..13708
                     /gene="mdcA"
                     /locus_tag="IVY71_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13810..14109
                     /locus_tag="IVY71_001222"
     CDS             13810..14109
                     /locus_tag="IVY71_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP7985288.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14099..15775
                     /gene="mdcD"
                     /locus_tag="IVY71_001223"
     CDS             14099..15775
                     /gene="mdcD"
                     /locus_tag="IVY71_001223"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP7985289.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15779..16372
                     /locus_tag="IVY71_001224"
     CDS             15779..16372
                     /locus_tag="IVY71_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP7985290.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16396..17472
                     /locus_tag="IVY71_001225"
     CDS             16396..17472
                     /locus_tag="IVY71_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP7985291.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17497..18366
                     /gene="mdcB"
                     /locus_tag="IVY71_001226"
     CDS             17497..18366
                     /gene="mdcB"
                     /locus_tag="IVY71_001226"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP7985292.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18477..19241
                     /locus_tag="IVY71_001227"
     CDS             18477..19241
                     /locus_tag="IVY71_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP7985293.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19730..21823
                     /locus_tag="IVY71_001228"
     CDS             19730..21823
                     /locus_tag="IVY71_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7985294.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21848..22150
                     /locus_tag="IVY71_001229"
     CDS             21848..22150
                     /locus_tag="IVY71_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7985295.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7178 a         3788 c         4725 g         6483 t
ORIGIN      
        1 tgtttgttcg ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc
       61 gcttttttgc gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc
      121 tacgtggatt tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg
      181 ttacagataa acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa
      241 tgaaagatat ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt
      301 ataaaatgtt gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc
      361 cttcagatat gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc
      421 gcgaccatat gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc
      481 catggcattc agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt
      541 tattagctta caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta
      601 tggcaacttc gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga
      661 cagacggatg gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt
      721 acgcccgttg catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga
      781 aaaaaatgtc tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg
      841 aaagctttac ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa
      901 atatccaaag attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg
      961 cgttagatat ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt
     1021 atcgaatgga tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc
     1081 caattctagg tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag
     1141 ataaaatcgt ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg
     1201 ttgatgccgc atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg
     1261 tgccgtatgc ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa
     1321 cgattgacaa agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga
     1381 tagatcctca taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata
     1441 aaaatatgag aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg
     1501 ctccagatat gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg
     1561 cagtctggac agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg
     1621 gggcttcaat cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg
     1681 ttaaaggaaa aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt
     1741 gggtattcaa agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa
     1801 tgttcgatcg gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc
     1861 atactacatt tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg
     1921 gtctaacgaa agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca
     1981 tgactcctta tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag
     2041 caatggaaaa gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa
     2101 caagagacat atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct
     2161 gtttttcata tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc
     2221 gttcgtttca tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac
     2281 tgggttttgt actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga
     2341 aaacaaaaac atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca
     2401 aagcaaatca cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga
     2461 aatattccag atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta
     2521 ttattgtacg cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa
     2581 aaaggtggtc cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc
     2641 tcatggctgt tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct
     2701 ccgttgtttg gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta
     2761 gttgtcattt tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca
     2821 aaaatcggcg gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc
     2881 ttattagggt ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt
     2941 ttttcatggg acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg
     3001 ccgatcatgt ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa
     3061 aatccagtta aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc
     3121 aatattttga atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat
     3181 aatatcgcgc aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg
     3241 aatctgttta gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca
     3301 ggaccagcaa agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat
     3361 ttttggaaga cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt
     3421 atctcaatct ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg
     3481 ctcgtaaatt caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt
     3541 ttgagattgc gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag
     3601 aaaaagagca atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt
     3661 tctgtcggac tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc
     3721 agtgtcattt tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa
     3781 caggaagtcg ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat
     3841 gaaacggact ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac
     3901 aggattattt ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt
     3961 ttatttgata tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt
     4021 agcaaatagt acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg
     4081 ggcatttttt gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt
     4141 aacagcaata ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg
     4201 aggtagcgca ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga
     4261 attggaatct tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg
     4321 tataggattg agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg
     4381 acagatcgat gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt
     4441 tttattcagt tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc
     4501 gcaaaagaat ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat
     4561 aggaacgatt ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag
     4621 tcctttagtg actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct
     4681 aatgattttg gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat
     4741 tttatatcaa acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa
     4801 aggcgtacct ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct
     4861 acattttttt gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag
     4921 ttgttttcta tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa
     4981 gaaacggaac actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat
     5041 tgctttcagc ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg
     5101 gtttgtattc ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca
     5161 gatacatttt gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa
     5221 aacttccctg cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac
     5281 agattgacgg tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa
     5341 gaagaacgac agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag
     5401 acaaaacgat ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc
     5461 attgcctctt ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc
     5521 gtagtgatcg tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg
     5581 aatggtttga agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa
     5641 aaaatccctg cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc
     5701 gttccagcag atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg
     5761 acgggagaat cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc
     5821 ggcgaccaga aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt
     5881 gtcgttgtag aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg
     5941 gtcgaacaac aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt
     6001 caaggcattc tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg
     6061 gattggcata tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga
     6121 ttgccagctg ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag
     6181 gcgatcatca aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca
     6241 gataagacag gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa
     6301 gaagcctgcg tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag
     6361 aaaacagaaa atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca
     6421 gccaatctac ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat
     6481 atggcaacga tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa
     6541 gtattatttg ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct
     6601 gcagaatttg cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg
     6661 aaacaggaac tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg
     6721 atcgatccgc caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc
     6781 tcagtgaaaa tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt
     6841 ggcttaaaac atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa
     6901 gagttgattc agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa
     6961 ctgcgaattg ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc
     7021 gtaaatgatg cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt
     7081 agtgaagtaa gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt
     7141 gcaaaagccg taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc
     7201 ttcttgccaa ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc
     7261 ccgctacctt tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta
     7321 tcttatgcac tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat
     7381 ccaaagcaag gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg
     7441 atcatggttc ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc
     7501 attcttctgc aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg
     7561 tccaagccgt cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt
     7621 atccttgctt tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt
     7681 acagtaagtt taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc
     7741 ttagttgtag aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca
     7801 gctagagaat acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa
     7861 acgattgagc tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga
     7921 atctagaggg aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc
     7981 ggttagttcg ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg
     8041 caagaacgcc ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg
     8101 tttgatagaa aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg
     8161 ttcattgatt tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa
     8221 gtgtgcatat tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc
     8281 aatcaatatc ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga
     8341 tgtttttttt agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag
     8401 gacctctaaa aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt
     8461 gtaattccaa ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata
     8521 aataccttcc gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct
     8581 gttggtatgg atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa
     8641 gaaccaacat gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt
     8701 tcttaaaaat tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt
     8761 atgagtgaaa ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag
     8821 aatacaagct gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt
     8881 gattttagtc acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc
     8941 agggacagca actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg
     9001 tttgaaaaaa atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat
     9061 tcctgcttga tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag
     9121 gtcagaaaaa atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat
     9181 ctttgtatga ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt
     9241 aatgattttt ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga
     9301 gaaaataaac ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc
     9361 atttactgat gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa
     9421 ataggtgggc aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc
     9481 ggattgaaca tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta
     9541 cttgtattca tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag
     9601 agatggaagc agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat
     9661 ttgtctccag aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa
     9721 agcaaaaaat ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca
     9781 agtgaccata ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt
     9841 ttgcgaggaa aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct
     9901 attgatctac gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt
     9961 aaatcaacgc aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct
    10021 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt
    10081 attttctaag aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat
    10141 tttgtcccag ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat
    10201 cattttgatc aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa
    10261 gggcaaatct ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac
    10321 ttttgggaaa aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc
    10381 atctgtcgtc gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt
    10441 atgacggaag gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa
    10501 ttgcagatgt tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc
    10561 tttcatgata ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg
    10621 atcggtaata tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt
    10681 ttccataaaa attcggacca agttcggaat agaaacagag gctacctcta aatacaatat
    10741 gacggaagta acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt
    10801 gtctaatgtc ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt
    10861 tctgccgcta tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg
    10921 gctcaaggca ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc
    10981 gaatactttt ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac
    11041 ctttttgtta tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga
    11101 tagcagtgga aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc
    11161 ctggacaagg cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac
    11221 gtttagaaaa tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg
    11281 tttttatcca gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa
    11341 acggtattca gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg
    11401 cttgtcgatc tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa
    11461 tgaaagaagc ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg
    11521 aagctgagga aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg
    11581 aaaattgtcc gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg
    11641 cagaagccaa acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc
    11701 actgtctctt gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc
    11761 tatctgatgc tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag
    11821 aagagattcg taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga
    11881 tggacttggc aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt
    11941 tgacaaagct gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag
    12001 accaacatgg tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg
    12061 aaaaaccaaa ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa
    12121 tcagcaactt gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa
    12181 aactgattgc tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt
    12241 ttctttcaga aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca
    12301 tgtctagtat ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga
    12361 tcgatttctc ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca
    12421 caatgaaatt aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt
    12481 tgattccaaa tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca
    12541 caggtttcaa tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta
    12601 tcgtgatcgt tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg
    12661 actgggtaga cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac
    12721 gagatcctca aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa
    12781 tctacgcaga acacggcgta caatctttga atcacggtat tggattcaat acagcagcta
    12841 tcgaattgtt actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga
    12901 actgggtatt gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa
    12961 gcatccatag ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg
    13021 tcttttttgt cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag
    13081 gacaatatgc catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact
    13141 cttctaccgt aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata
    13201 atcctggcgg tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc
    13261 cattagggaa aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa
    13321 aaccagtctt tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca
    13381 atgcaatgca ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg
    13441 aattgcttat ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat
    13501 tggcggtgtt acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc
    13561 agccggcatc gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc
    13621 attattagct gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc
    13681 accaatgaaa ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta
    13741 cccagatgga agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg
    13801 gagagatcca tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa
    13861 atccatgtag gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa
    13921 acagaatcta ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat
    13981 gtattaacga gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc
    14041 ggtgctacac caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat
    14101 gaaaaatagt tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg
    14161 acgtgaattg attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat
    14221 cgtccctgaa agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc
    14281 agtagtaatc gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc
    14341 aaaaatcgtt gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt
    14401 tccagtcatt gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt
    14461 atctatttca gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg
    14521 tttgattcca ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag
    14581 ttatttaatc gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca
    14641 agaagcaggc gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg
    14701 atctcgtcaa cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc
    14761 aatcaaagct gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag
    14821 ctcagcattt tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga
    14881 cgattacaat gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc
    14941 agaaggaaat gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg
    15001 catgaaaaat ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat
    15061 agaagaagcc atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg
    15121 aaacggtgaa gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta
    15181 cgaagaagat aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag
    15241 ccaagcatac ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc
    15301 tgctgcttat gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa
    15361 tgctatttca ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa
    15421 caataaagaa attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg
    15481 ttcggttgct gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa
    15541 taattataca aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc
    15601 ttcagaacaa aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg
    15661 caatacaaat gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg
    15721 acgtgtcgaa acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat
    15781 gattcaagca catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc
    15841 tgaatgggtc gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca
    15901 cgcagaaata ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga
    15961 aatctcaaaa catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc
    16021 gtatagacaa gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa
    16081 catattatca ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg
    16141 gattccggca gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat
    16201 tgaattgcca attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca
    16261 agtagtgact cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa
    16321 aattctattg aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa
    16381 aagagagaga aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag
    16441 aaagtatcag ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac
    16501 agccacagcg caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca
    16561 gcaccaatgt ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct
    16621 tattgggaat ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg
    16681 acggcttatc tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca
    16741 agctttttac tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg
    16801 atgctgcaga tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct
    16861 aataaaaaat atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat
    16921 ctgttctatc aaacacaagt attgactggg ccttatcatg acagctggta cttattagat
    16981 cggtttgttg ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc
    17041 tatcaatatt tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc
    17101 ttttctgtcc gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac
    17161 ttgaatacga tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct
    17221 tccaaaatga ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat
    17281 gcttatcgta cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa
    17341 gatagttggc tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca
    17401 agttgtgcat ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt
    17461 ttcagcaagt aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa
    17521 gcagtgactc agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta
    17581 agtccaaaac ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat
    17641 gctttatttc ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata
    17701 gcatggcatc atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa
    17761 gcggaaaagg caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg
    17821 gcaatggggc tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct
    17881 ttatcgaaaa tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt
    17941 cataaaaaaa ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat
    18001 gaagaagcga tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta
    18061 gataatgatt catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta
    18121 gatgacacat gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt
    18181 gctcatgctg ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt
    18241 tgtcaggaaa accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc
    18301 atgctgaaga tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat
    18361 gactaaataa gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct
    18421 tttagcaagg ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga
    18481 aaaaaatcaa ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga
    18541 cccaaggatt cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg
    18601 gcagaggttc atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg
    18661 gcagagattc ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg
    18721 gatttcttgc attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga
    18781 ataaaagaca aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta
    18841 aagaaaagaa agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt
    18901 gggaagaaga aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac
    18961 atcagaaagt tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa
    19021 ctattcagga agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg
    19081 atttcagatg ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta
    19141 agacacaacg acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa
    19201 gatggcaagc cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg
    19261 gagttgtgac aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata
    19321 gcctatcggc aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt
    19381 cttatcgctc aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc
    19441 ttgcatctgc gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt
    19501 tgttcttatt acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca
    19561 aaatttttgt aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct
    19621 tattttttgt ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt
    19681 tattatgtaa gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag
    19741 aacaattgaa atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct
    19801 agcaagtgag ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat
    19861 ctggttgagc agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt
    19921 gaagctggaa aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg
    19981 ctttttgaat cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat
    20041 cccgatccat ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga
    20101 agatatgaga cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg
    20161 aaagcaagga ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt
    20221 tatcaatcag aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc
    20281 ggctcaaaaa atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat
    20341 ctattccaat aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt
    20401 tacattgatc tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa
    20461 aaatacagaa ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt
    20521 tcaattggac aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt
    20581 caatgctaga gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca
    20641 attgatccaa tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt
    20701 attcgaaaat ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca
    20761 agtcatgaat ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt
    20821 cactcatttt gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat
    20881 agcttttctt gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta
    20941 tgtttataaa tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga
    21001 gaatctgaaa gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat
    21061 cgaattagta gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg
    21121 aaataagccg cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga
    21181 agactttttg aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac
    21241 ggagttattc actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat
    21301 catctatggc aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga
    21361 gatccagcct atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc
    21421 cactacttgg gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt
    21481 cgaagagcgc tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt
    21541 gattggcaaa cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg
    21601 agtcagcgta gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt
    21661 aaagattatt ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga
    21721 actaattgaa ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat
    21781 cgaagatttc aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag
    21841 aacaaagatg aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt
    21901 tgcagcacag atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga
    21961 ccattgtgat atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa
    22021 ttttcgttcg cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa
    22081 aaatattgtt tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg
    22141 agttatctaa atacaaaaag aaaagagggt aaaa
//
LOCUS       DACCXW010000039        21813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCXW010000039 DACCXW010000000
VERSION     DACCXW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.039"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="IVY71_001230"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="IVY71_001230"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7985296.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="IVY71_001231"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="IVY71_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7985297.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="IVY71_001232"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="IVY71_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7985298.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="hflB"
                     /locus_tag="IVY71_001233"
     CDS             complement(4246..6357)
                     /gene="hflB"
                     /locus_tag="IVY71_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7985299.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="IVY71_001234"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="IVY71_001234"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7985300.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="IVY71_001235"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="IVY71_001235"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7985301.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="IVY71_001236"
     CDS             complement(8557..9027)
                     /locus_tag="IVY71_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7985302.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="IVY71_001237"
     CDS             complement(9073..9498)
                     /locus_tag="IVY71_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7985303.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="IVY71_001238"
     CDS             complement(9565..9834)
                     /locus_tag="IVY71_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7985304.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="IVY71_001239"
     CDS             complement(9845..11440)
                     /locus_tag="IVY71_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7985305.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="IVY71_001240"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="IVY71_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7985306.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="IVY71_001241"
     CDS             complement(14993..15553)
                     /locus_tag="IVY71_001241"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7985307.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="IVY71_001242"
     CDS             15906..16880
                     /locus_tag="IVY71_001242"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7985308.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="IVY71_001243"
     CDS             17050..18450
                     /locus_tag="IVY71_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7985309.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="IVY71_001244"
     CDS             complement(18528..19250)
                     /locus_tag="IVY71_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7985310.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="IVY71_001245"
     CDS             complement(19329..19835)
                     /locus_tag="IVY71_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7985311.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="IVY71_001246"
     CDS             19958..21757
                     /locus_tag="IVY71_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7985312.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6053 a         4752 c         3911 g         7097 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ttgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttg
//
LOCUS       DACCXW010000040        21503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCXW010000040 DACCXW010000000
VERSION     DACCXW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.040"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            109..1875
                     /locus_tag="IVY71_001247"
     CDS             109..1875
                     /locus_tag="IVY71_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7985313.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1890..2327
                     /locus_tag="IVY71_001248"
     CDS             1890..2327
                     /locus_tag="IVY71_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7985314.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2340..3491
                     /locus_tag="IVY71_001249"
     CDS             2340..3491
                     /locus_tag="IVY71_001249"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7985315.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(3553..4251)
                     /locus_tag="IVY71_001250"
     CDS             complement(3553..4251)
                     /locus_tag="IVY71_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7985316.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(4244..5596)
                     /locus_tag="IVY71_001251"
     CDS             complement(4244..5596)
                     /locus_tag="IVY71_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7985317.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(5819..7219)
                     /locus_tag="IVY71_001252"
     CDS             complement(5819..7219)
                     /locus_tag="IVY71_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7985318.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            7380..8117
                     /locus_tag="IVY71_001253"
     CDS             7380..8117
                     /locus_tag="IVY71_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7985319.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            8242..8721
                     /locus_tag="IVY71_001254"
     CDS             8242..8721
                     /locus_tag="IVY71_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7985320.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            8718..9485
                     /locus_tag="IVY71_001255"
     CDS             8718..9485
                     /locus_tag="IVY71_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985321.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            9715..10284
                     /gene="lepB"
                     /locus_tag="IVY71_001256"
     CDS             9715..10284
                     /gene="lepB"
                     /locus_tag="IVY71_001256"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7985322.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10388..10990)
                     /locus_tag="IVY71_001257"
     CDS             complement(10388..10990)
                     /locus_tag="IVY71_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7985323.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            11096..11565
                     /locus_tag="IVY71_001258"
                     /pseudo
     CDS             11096..11565
                     /locus_tag="IVY71_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11671..12255
                     /locus_tag="IVY71_001259"
     CDS             11671..12255
                     /locus_tag="IVY71_001259"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7985324.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            12258..13331
                     /gene="prfA"
                     /locus_tag="IVY71_001260"
     CDS             12258..13331
                     /gene="prfA"
                     /locus_tag="IVY71_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7985325.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            13324..14163
                     /gene="prmC"
                     /locus_tag="IVY71_001261"
     CDS             13324..14163
                     /gene="prmC"
                     /locus_tag="IVY71_001261"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7985326.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            14156..14335
                     /locus_tag="IVY71_001262"
     CDS             14156..14335
                     /locus_tag="IVY71_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985327.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            14357..15382
                     /locus_tag="IVY71_001263"
     CDS             14357..15382
                     /locus_tag="IVY71_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7985328.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            15573..16817
                     /locus_tag="IVY71_001264"
     CDS             15573..16817
                     /locus_tag="IVY71_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7985329.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            16970..17917
                     /locus_tag="IVY71_001265"
     CDS             16970..17917
                     /locus_tag="IVY71_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7985330.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            18051..18680
                     /gene="upp"
                     /locus_tag="IVY71_001266"
     CDS             18051..18680
                     /gene="upp"
                     /locus_tag="IVY71_001266"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7985331.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(18819..19061)
                     /locus_tag="IVY71_001267"
     CDS             complement(18819..19061)
                     /locus_tag="IVY71_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985332.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            19182..19254
                     /locus_tag="IVY71_001268"
     tRNA            19182..19254
                     /locus_tag="IVY71_001268"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19215..19217,aa:Thr,seq:cgt)
     gene            complement(19373..20011)
                     /locus_tag="IVY71_001269"
     CDS             complement(19373..20011)
                     /locus_tag="IVY71_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7985333.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(20134..20766)
                     /locus_tag="IVY71_001270"
     CDS             complement(20134..20766)
                     /locus_tag="IVY71_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7985334.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            21117..21416
                     /locus_tag="IVY71_001271"
     CDS             21117..21416
                     /locus_tag="IVY71_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7985335.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         7342 a         3781 c         4322 g         6058 t
ORIGIN      
        1 ctgacatact caaacctttg gcacaaagtt tcgtgccaaa gtacgactat ttctcttttt
       61 agtaagcgga tacaattaga gtatcaaaag gagagaggga gagacaaaat ggaacggacg
      121 attcccaaaa acaacatttc gctaaaagcg agggtacaaa aattaggcag tactttatca
      181 agcatggtca tgccaaatat tggagcactg attgcctggg gtgtattaac ggctctattt
      241 atcccagatg gttatttacc aaatgaggct ttcgctacca tggtaagccc aatgctaact
      301 tatctgattc ctttattgat cggttatact ggcggaaaag taattgctgg tgaccgcgga
      361 tcagttgttg gggcaattgc aacgatgggt gtgattgtcg gaacagatat tccaatgatg
      421 ctaggcgcga tgatcatggg acctcttggt ggatatgcca tcaagaaatt tgatcagcta
      481 ttccaaaaac ggatcaaatc cggttttgaa atgctagtca ataatttttc agcaggtctt
      541 atcggttttg gattagcgtt attaggtttt tctgcgattg gtccagttgt cgatgcattg
      601 actcaagcaa tggcaaaagg tgtagagatc attttatctg cccatttgat tccattgaca
      661 agtattttta ttgaaccagc aaaaatctta tttttgaata atgcaatcaa tcatgggatt
      721 ttgacaccat tgggaactga acaagtagca gaggctggaa aatctattct attcttgctt
      781 gaagcaaatc ctggtccagg acttggcgta ttacttgctt ttactttgtt tggcaaagga
      841 gcagcaaaat cttcagcacc aggagccatg atcattcact ttttaggcgg gatccatgaa
      901 atctattttc cttacgttat gatgaaaccg ttattattct tatcagttat aggcggagga
      961 atgtcaggaa gtttcatttt ccaattgatg gatgctggac taagagcgcc agcttctcca
     1021 gggtcgatca ttgcgattct agcgatgaca ccgttcaatt cttaccttcc tgttattttg
     1081 ggcgtgattg ttgccgcagc tgtttcgttt ggtatttctg cagtaatttt gaaagcggat
     1141 gcgaaagata caaatgaaga cttcgaaaaa tctgttcaag agacaaaggc agctaagcaa
     1201 gcttccaaag gtgcccaagg tattcctcaa ggagctacat tctctggcgt acaaaaaatc
     1261 atttttgctt gtgatgcagg aatgggatca agtgcgatgg gcgcttcgat cttgagaaaa
     1321 aaagtacagg aagcagactt gcctcaaacg gtgaccaatc aagcaatcag ccaactgaca
     1381 gatgactcga atacattgat cgttactcag gtcgaactac aagaaagagc aaaacaaaaa
     1441 gcaccgagcg cgcgttttgt ctcagtagaa aactttttga attcacctcg atatgatgaa
     1501 atcattcaag aattaagcag caagcagaaa acatcagttt ctgaaattga gaatgagcca
     1561 gcagaagagt ttctttcagc aaacatcaaa caaatcaaag agattctact tgtccacgat
     1621 gatcgttccg gatcagcaac aatggggatg accgtgctca aagatatatt agacaagaac
     1681 catttaacta tcccgttaag aaaagtcaat atccaagatt taaaagatga acctgattca
     1741 ctgatcgtag ccaaagaaag tttagcggaa aaagtacaga tggctgcacc tcaatccatt
     1801 catttatcag tcaacagcat ggtcaatccg caaaaatacg aatcgattgc acaatatttg
     1861 aaacagacag cttaagggag gaaaacagca tgcaaagttt gccattcgac atgatcgagc
     1921 tcaatcgatc ttttaaaacc aaagaagaag cgatccgcta ttgcggtcag atgcttgtag
     1981 aagcagggtg cgttgatgag gaatatatcg atgccatggt agatagagac aaaatgttat
     2041 ctgtttatat ggggaatttt atcgcgattc ctcatggaac agatgaagca aaaaaatatg
     2101 tgaaaaaatc cggcatctgt gtcgttcaag taccagatgg tgtagatttt ggtaccgaag
     2161 aagaagaaaa gattgcgact gttttgttcg gtattgcagg agtcggagat gagcatttgc
     2221 aacttgtaca gcaaatcgca ctttattgca gtgatatgga caatgttgtc cagctagcag
     2281 acgcgctcag caaagaagaa gtaacggaaa atctagcaat tgcttaagga gagagaaaaa
     2341 tgagagcagt acattttggt gcaggaaata tcggtcgagg atttattgga gaggttttgg
     2401 cagctaatgg atttgagatt actttcgtag atgtgaatca cacgatcatt gatgctttaa
     2461 atgaaagaaa agaatacgaa atcgaattag cagatgaatc aaaagaaaaa atcactgtaa
     2521 aaaatgtcaa aggaatcaac aatcaaacaa atccagaaaa agtaatccaa gaaatcgctg
     2581 aagcagattt agttactacc gccattggcc cgaatatctt accttttatt gctgagttga
     2641 ttgcaaaagg tatccaagaa agagaaaagg aaggaagcac aacatcttta gatatcattg
     2701 cctgtgaaaa tatgattggc ggttcagcat ttctagaaaa agaagtgtat aaatatcttc
     2761 cggaaacatc atttactgac aaatatatcg gctttccaaa tgcagcagtc gatcgaatcg
     2821 tcccattaca acaccatgaa gatcctttat tcgtccaagt ggaacctttc aaagaatggg
     2881 tcattgacga tagccaaaga aaaaataaaa aaatccaatt aaaaggggtt ttatatgtag
     2941 atgatctaga accatatatc gaacgcaaac tatttagtgt caatactggt catgcgactg
     3001 tcgcttatac aggtgccttg ttaggttacc agaccattga tgaagcaatg caagatgcgt
     3061 tagttgtggc acaattaaaa tcagttttgc aggaaacagg aagtttgctg attgccaaat
     3121 gggggtttga tgcagagcag catcatgcgt atattgaaaa aatcattcat cgcttccaaa
     3181 acaaaaatat ctctgatgcg atcacacgtg tcgctcggac accattacgt aagttaggtt
     3241 atcaagaaag atttacacgt ccagtgagag aactacaaga acataacttg acttgcccac
     3301 atttgacggc aacaatgggg attattttta attactatga tcctgaagat gaacaaagcc
     3361 gtcaattgca tgaaatgaag atccatgaaa acttggaaca attgattcaa gaagtcacag
     3421 gaatcaatga tccgaaaaca atcgggaata tcaagcaaaa cgtgaaccgt tatgccaagc
     3481 aagtcgctta aaataggata cgaaaaaggg gaatgtgacg aaatttttgt cacattcccc
     3541 tttttcgaat aactaggctg tttgtccttc aagatagggc agatccaatt ctttaccgta
     3601 acgttgttct aaagctaggc ggatcaaacg agctgccagg ttctcatttc ttgcaaggat
     3661 aggtccatgg aaataagaac cgaagacatt tcggtaaatg acaccttcag attggtcttc
     3721 accattattt ccttgacctt gtaccacttt ccccaaaggt cgttcacctt cacctaagaa
     3781 agtccgacca ttgtggtttt caaatccata ataagtttca ttgaattctt cgttatggat
     3841 aacgatatca ccgataaacc ggttattttc ctgacttaac gtgtagtgat ccagtgcact
     3901 gattccttgg atcttttctc cattagctcc catgtagtag tgacctagca attggtagcc
     3961 accacaaatg gctaacatga cgccatcatt ttcgatatag ttgatcaaag aatctttctt
     4021 tttctggatg tcttttgaaa tgattaattg ctcaaagtct tgaccaccgc caaagaaaac
     4081 caagtcatat ttatcagcat caaaaggttc atgtatacta acgatctccg tatggcaaga
     4141 aacccccatt ttttctgcta tatatttcaa catcaaaagg tttccgttat caccatatgt
     4201 gttcatcaaa tttccataaa ggtgggcgat attgagttca taattagcca cggttcattc
     4261 ctcctttgat aaatccttga gcagctagtt ctttacgaag ctgcaataca gcagtgtaag
     4321 tagctaaaat atagacatgc tcagttggca attctttgat tttttcgatt acttctggta
     4381 attcttttgt ttctgtcaat ttattttctg gaatcccggc aacttttaga cgtaaagtca
     4441 tatctgtatg acggtctcct ccagaaataa cagctgggat atccatatcg gcaaagtttt
     4501 caaaattacc atcccaaatc cagctgacat cgattccgtc tgcgtaattg gcatttaata
     4561 aagccactaa agaaaatgga taaggagcta gaccaatcat atcgattact tgattgatac
     4621 ctacaggatt tttgacaagt actaatgtac attttttccc attgatattg atgacttctt
     4681 gtcggccgaa tactttctcg tcatatccta aaccttgttt gattttatca ggatcgacat
     4741 tgaagtactc tgccactgca gtagctgcta aagcattgta gacattatac ataccgccaa
     4801 cttcgattcc gtattcatga ccatcgatca cgaaatctgc tgacacattg tccatccgta
     4861 ccatatcagt taatcgataa tccaattcag gacgtttgaa gtcacaattt ggacagtaat
     4921 atttccctaa gtttgcataa gtgatcattt tataatgcaa gatgtgatga caatttggac
     4981 ataagacacc gtctgtattg taatgagcca tttgttcacg atcttcttca tggtcaaagc
     5041 cataatattt tctaggattg actgtttcga tagaattgaa gattggtgaa tcaccgttac
     5101 tgataatcgt tgcatttggt gactttgcgg caccgtctac aatcatttta taggtcgtat
     5161 agatttctcc ataccgatcc atttgatcac ggaaaatatt tgtaaataag aacagttctg
     5221 gtttcatata ttctgtgact ttgctcaaac ttgcttcatc tatttctagg acagcaaact
     5281 ttttctgtcc tttttttggt tttgcctgca agaaagtcga tacaattccc tgtaccatat
     5341 tcgcaccagt agggttggtt agaacttcat cgaattgctg gcgcaaaata ttgactgtta
     5401 aagcggtagt caatgtttta ccgtttgttc cggtgacaac gacgacttgg tagtcttttg
     5461 ccaaactatc tagtatatgt ggatcgactt tcagcgctag tttacctggt aaacttgatc
     5521 cgcctttaaa aaaagttttt aatgcccatt gcgacgtttt tccaactgca atcgcaaaat
     5581 gactgcgtat tcccattatg taacctccaa aaacgatttg ctgacgaata aattcaacta
     5641 atcataccat atgttgaaaa aaaggttaaa ttgcttttct tattcttaag aaaaggttac
     5701 ggctgaaagt gcatgaaatc ggacaaaatc gcatgattta acaaaaaaac agcttgaaac
     5761 aaaatgattg tttcaagctg ctgtgatttt attattccct cttacctata ttcccatttt
     5821 aaccgataat aattttctcg cttggatatt catagcctag atctcgtgtt tcttttggca
     5881 cgaataacat caatgtatat agcattccga tccgcccaat aaacatcagt gtagcaatcg
     5941 tgattttgcc cacaacagac aagtctccgg taatccctaa agataaacca gttgttccaa
     6001 acgcagaagt tacttcgatg atgatcgaga tcagcgtttg ttcttctgta gcagataaaa
     6061 aaacgataca aaagaaacac ataccaagag aaagcataaa gacaacgact gattttcgca
     6121 catcatcttg atcgattctc cgtccaaaaa tattgatgtt atcttcactt ttgaggaagg
     6181 aatatagata caaaccgatg attgctacag tagttgttcg aatcccgccg cctacagaac
     6241 taggactgca tccgataaac atcagtaaag aaaaaatgat cagtgttgtg atttgaaaat
     6301 cacccaaatc gtgaatctgc agaccagcat tacgagtcgt aatcgaataa aacatcgaat
     6361 tgatccattt gacactcata ttggaatctt ggaataaatg gtccttttcc aatagataaa
     6421 tcaagacagt tcccgaaaca aacaagataa caaaagctaa cacagctaat ttggtaaaca
     6481 aggaaaaacg aaacggaagc ttcgcattgg aacgtttgta taatagccat tcccggcact
     6541 ccatcaatac aggaaaacca attcctccga caaaaatcaa aaacatgatc aggatcaaga
     6601 aaaaatagtc atgtgcaaag ggcttgatgg aatccccagt gacgtcaaac ccagagttcg
     6661 tgacagccga tatcgcctga taaaaaccgt aaaaaacggc atcacgccaa cgatcaaaat
     6721 agcctctgta ataaaaataa atagaaaaga atgtcccaaa aagtaattga aaccagatta
     6781 aaatagcaaa agtgatccga atcagtcgga cgataccaga caaacgcggt tgattcatat
     6841 ccgtcatgat cagctgtcgc tgtttcagtg tgatccgtcg tttagaaaaa atgacaaaag
     6901 cagtagaaat catcatgatc cccaaaccac caacttgaaa taatatttcc agcagtataa
     6961 taccgttatc attgaacaca gaattgatat caaaagtcga tagtccagtc acactcactg
     7021 tactgattgc catgaacagc atgtccacaa aagaaacatg gctatctggt tcgcgaaaaa
     7081 tcggcatata aaatagaacg agtgataaga ccgtcatcaa tatataataa aaaacgatga
     7141 tttggatcga tgagaaatga ttggcagcaa acttctgtcc tcgccgctgc agccttcgaa
     7201 agaataaatc aacatgcaca gtactatcct cctgacaata gtcgtttcta ccagtataca
     7261 aggggaatta ggaatactca aagaaattat caaacagata acaatacagc tttcgattaa
     7321 aatagaaatg gcttataata aggaaaatcc tagaaaaaag aaggaggagg aataacaaat
     7381 tgattaatgc tcaacaaatc gtagaaaaaa tgaaaaatca aaaaataaat tatgatacag
     7441 ttctgaaaaa aatgatcatg caatgggaga aagaagaagt aaagcctaag atattaatac
     7501 atacatgctg tgctccttgc agtacgcaca cattggaatt catgtctcaa tatgcagaga
     7561 tcacgttatt tttttcaaat tcgaatattc atcctaaaag tgagtatttg cgaagattac
     7621 atgaacaaaa acgatttgta gaccaattta atgagaaaac tgaccatcat gttgcgctaa
     7681 tcgttgatga ataccgtcca aatgaattca ccaaaatggt tttaacaaac catcttgaaa
     7741 acgaaaaaga aggtggtgct cgctgcagtg catgcttcaa tatgcgatta gatcttgttg
     7801 cgcaaaaagc gcaagaacta ggatacgatt actttggaag tgccctgacc atctcgccaa
     7861 agaaaaatgc agctttgatc aatcagatcg gaatggatat ccaaaagatt tacgggacac
     7921 aatacttgcc aagtgatttc aagaaaaaca aaggctatga acgctctttg caaatgtgca
     7981 aagagtatga tatttatcgc caatgttatt gtggttgcgt gttcgctgcc aagcagcaag
     8041 gatgcgatct gaaagaaatc aatcgggaag caaaagctta cttaaaaaca tcaaccgtag
     8101 tttttgagga aaattaaaga aatctttcgg aaaaacgtaa gaaaaaaata aagaatttat
     8161 ggtagaaata aggtagactg tataatttgt ccgtagtaaa gtaaggaaac aagaataaat
     8221 ttcaatgaag gaagagataa gatgcgaaaa gtagcaccta ttttgaagcc aatcgctgta
     8281 ttaacaatct cgacgattgt cgcgactact acaggtattg tgaatagttc cagctaccgt
     8341 gcaccaagtt caagttctag tactgtagaa gaaacaacac caactacttc agtctcgaca
     8401 tctgaaccaa caacaacgga aagtttgaat gaaaccacta cgacaagtac atgggacgat
     8461 acaacaacta gttcgtcgta tgatgagaca tatacaactg acagctcaag cgaaacaagt
     8521 aatacggtac cagatggcac tccatcggat tcaaccaatc caagtacaag tccggataca
     8581 agtgctagct ccgaaacgat accaagcagt acgactgtag atgataacac gcaaaatgat
     8641 gatgcagcac gtacagcaca gcaaaaccaa gattcaggaa atacccaaac gtataaccag
     8701 caagcaggtg attctcaatg acatttttac tagaccttat caacggggtg cataaattcc
     8761 ttggctattt agacatcagt cctaaatatc ttaaccgtgg atatacgatt ttaagtgtga
     8821 ttcccacgct ttatttattg agaattgttt atggactttg gcaaaaccaa aactatcttc
     8881 aatttttctt atatggactt gcatttctag tgcttgttta ttttactgtt ttaaatgtat
     8941 tttattactt tttagataaa aacacgaaag ccgatgtcac tcagcttttt gtgaagtatt
     9001 tacctgatga agcgttcaac atccagacag aagaatcgat agataatgga aattcgatcg
     9061 ataaagtgaa tacacaagag gtcatggttt cttatgacga agattatcaa ttgaaattag
     9121 ccgagaatat gcggtatttg attggaaatg gtgagatcaa aacaaatgaa ctcggtcgca
     9181 tggatggatt tctggtcgat agaaatacat tgtatcctta ttactatgtc aagaaaacag
     9241 gagagaaaga gtatgttcta aatattggac gaagctactc ggacatggaa aaagtaggga
     9301 ccattcgact tgactcaaca gaagacacat tggaacctgt cggattgttc atcgtcggcg
     9361 gtgattttgt caaagacggg ttacgctatc atgagcctta tcgtctgaaa ttgattgcaa
     9421 aaaaggcaca gtcgcaggaa gaaccagtgg tttatagtcg tagccaaaga agaagacatt
     9481 cataatgtga tgaaacgccg tgtatcagtg gttgcgataa aaacgtttat tcggaataag
     9541 ggggatgtga cacgtctttt gtcacgttcc ttttttatat aacgagaaag ataagacgaa
     9601 cacatcactt attctttcct ttcatttata aacattgtag aataaagaaa gtacaacgaa
     9661 gtattgtgaa aaaacgtaat aaataacgag gaaaatacaa gtaggaggta agctgtgaaa
     9721 gaatttttga aaaattgggg gattttgatc cttgtattag cggctatttt attagctaga
     9781 gtgtacgtgt ttacaccagt tactgtaaac gggcattcga tggaccctac attaagtgat
     9841 ggacaacgat tgatttcatc taagatctca aactatgaac gaatggatat tattactaca
     9901 aaggaaccgg gggatgaaga acggatgatt gttaaacgga tcatcggtat gcctggagat
     9961 acagtcaaga tggaaaatga ccagttgacc attaatggca aaaagtatga tgaaccttat
    10021 ctggatgagt tcaaaaaaga attttcagaa gataagttac aaggagaata tgcttatagc
    10081 tcaggattcc aagcacaagc agaaagttca tccaccttta cgagtgactt tgaatatact
    10141 gtgccaaaag gaaagtatct tgttctaggc gataaccgct tgatttcaaa agacagccga
    10201 atgtttgggt tagtcgacaa agatatgatc caaggaaaag ttgttttccg ttattggcct
    10261 ctttcagaaa ttaaaatcat gtgatgaaaa aataaaaaga agctgtgaca tgttttttgt
    10321 cacagcttct ttttccgaaa attccttctt atttcttgga gttgctcact ttttttgcaa
    10381 tctcatttta agctttttgg ctgatggata agaaaagcga agcgtttttg ttcataaata
    10441 gcttttcccc acgatggaag gtatgactag tagcagagac aaagcccgtt ttttccaatc
    10501 gattattcaa ttcttcttgg ctaaagccat tatggacttt cggatgacta atttgttcat
    10561 tcttgtcaaa gtctacgatg atcaattgac cgccaggagc taaaatttca taaagttttg
    10621 taagaatatt ctccgtatca ggaatgtgta aaagtacaag tgatagaagg atgagattag
    10681 cttggacagc aggaaattcg acagaagcat cagcgtggat cgttcggaca tttttcagat
    10741 ctgctgcttg gatcttttct tctgccattt tcagcatagt ttctgaagca tcggcaatga
    10801 tcagttcttt gaatctttcc gcaagcggta aactaacaag cccagttccg cctccgtaat
    10861 ccagcaggac tttttggtgg ctgtcgcgcg gtagataagt agttagttct tcgcgaataa
    10921 tcttggctaa tgcttgacgg tcaggggaat cataatggtt agcgatggaa tcaaaaatat
    10981 tttttgtcat caaatagctc cttacgtaaa actttgaaat tttaacttat tatagcacgt
    11041 gaaaagaaag aaagcatgct atactcaatt caaaaaggga gaatctagaa agaatatgcc
    11101 gatatcgttt gtaaaagata gagaggaaaa aggaaagtgt gtccgagaaa tattactaga
    11161 cttgcctgag tggtttgggc ttcctgaatc aacggaaaaa tatatcgagg aatcaagtaa
    11221 actaccttta tggtgtgaaa aaagaaaaga agaatattta ggctttatca ctctttcaca
    11281 gacaagtgaa gatactgccg aaatctattc tattgtatgg gaataaaaaa agactatcat
    11341 caccaaggat taggaacaaa actttttaaa gaagcagaag gttacgcagc aaaacattac
    11401 aaatatttgc aagtgaagac tgtagacgag gggcactact ctatatatga tcaaactatt
    11461 tgtttctacg aatcattagg attttcaagg ttggaagttt ttccaaattt atgggatgaa
    11521 tggaacccat gtttagttct tgtcaaaaaa ttggaacaaa agtgagcaat agatttaata
    11581 ctaatctgtg cttgatgaaa attctcactg taaaaaatgc tgaaaatcaa gtataatacc
    11641 ataggaacaa actggaaggt gaaaaagagc atggcacaac tattttttaa gtatggcgca
    11701 atgaatagcg gcaagacgat cgagatccta aaagtcgcac ataattatga agaacaaaat
    11761 aagccagttg tcttaatgac aagcggtatt gatacaagag atggtttagg cgttgtttca
    11821 agccgaatcg gactgaaacg taatgcgatg ccgatatttg aagaaacaga cgtatttgac
    11881 gtaattcaga aaatggaaag caaaccctat tgtgtgctga tcgatgaagc gcaattctta
    11941 aaaaagaaac atgtcattgc ttttacgaga attgtggacg aattggatat tccggttatg
    12001 gcatttggtt tgaaaaatga ttttcgaaac gagttatttg aaggatcaaa atatttattg
    12061 ctttatgcag acaaaatcga agaaatgaaa acaatctgtt ggttctgcca caaaaaagca
    12121 atcatgaatt tacattatat tgatggaaac cctgtttatg aaggcgatca agtgcaaatt
    12181 ggcggaaatg aagcttatta ccccgtttgt cgaaaacatt actttcaccc atcaatgatt
    12241 acagaaggag aataagcatg tacgatcaat tacaagcaat tgaagaccgt tatgaagagc
    12301 ttggagaact tttaagtgac ccagaagtga tttctgatac aaagcgtttt atgcagcttt
    12361 caaaagaaga agcaaacacg cgcgaaacag tcgaagttta tcgtcgttat aaagaagtag
    12421 tacaaggaat caccgataca gaagaactgc ttggtgaaaa actggatgat gaaatggctg
    12481 aactagcaaa agaagaatta tctgaattaa aaaaagaaaa agaagtctta gaagaaaaaa
    12541 tcaagatctt gcttttacca aaagatccaa atgacgataa aaatatcatc atggaaatcc
    12601 gtggagcagc aggcggagac gaagcagcat tattcgctgg cgatcttttc aatatgtacc
    12661 aaaagtatgc tgaagcacaa ggctggaaaa cagaagtgat ggaagctaac gttaccggaa
    12721 tcggtggata taaagaagtg atcatgatga tcaccggaga caacgtatat tcgaaattaa
    12781 aatatgaaag tggtgcgcac cgcgtccagc gtgttccttc aactgaatcg caagggcgta
    12841 tccatacttc caccgcaaca gttgtcgtga tgccagaagc tgaagaagta gagatcgata
    12901 ttgaagacaa agatatccgt acagatatct atcatgcttc aggtgctggc ggacagcacg
    12961 tcaataaaac agcatctgct gtacgtttga ctcacttacc aacagggatc gttgttgcta
    13021 tgcaagatga acgttctcaa atcaagaacc gtgaaaaagc aatgaaaatc ttgcgtgctc
    13081 gtgtgtacga taagatccag caagaagcac aaagcgagta tgatgccaat cgaaaatcag
    13141 cagtcggaac tggggatcgt tcagaacgga tccggacgta taatttcccg caaaatcgtg
    13201 tgactgatca tcgtatcgga ttaactatcc aaaaattaga tcagatttta gcagggaaat
    13261 tagatgagat cgttgatgca ttgatcatgt atgatcaaac atcaaaactg gaagctatgc
    13321 aaaatgggta aaatgaccta tcgagaagtc ctgtcacggg cttcttcttt tttagaggat
    13381 catgggaaag aagcatatag tatccaattt ttatttttgg aacgaaaaca atggaaaaaa
    13441 cttgattggc tccttcatat gaacgaagag atttcagaag aagaacagcg gttgatcgaa
    13501 accgatctgc gacttctgct tgcagatcat ccacctcagt atttgctagg atatgcagat
    13561 ttttatgatc atcggttaaa ggtaactgaa gcaacactga tccctcgacc agaaacagag
    13621 gagttagtcg aatggtgttt agacgagacg cctggtgtac cgttagaagt gatagatatt
    13681 gggacaggga ctggagcaat tgctattagt ttgaaagcag caagaaaaaa ttggcatata
    13741 tctgctgtgg atctttcaga agaagcatta gaagtagcga aagaaaatgc tcagaaagaa
    13801 ggaacgaaaa tcagttttta tcatggagat acattaggac cagtaatgga tcagcaattt
    13861 gatgtgatta tttctaatcc gccgtacatc agtcggaatg aatgggaact gatggatgaa
    13921 agtgtccggt cattcgaacc taaaatggcc ctttttgctg agaacgatgg attagctatt
    13981 tatgaaaaaa tagcagttga agcaccaagc gtactaaaat caaatggaaa gattttttta
    14041 gaaatcggat ttcgccaagg tgaagcagta aaaaaaatat ttcagcaagc atttccagat
    14101 aaaaaagtgg cgataaaaaa ggatcttttt ggcaatgaac gaatgatcca agtttatggt
    14161 tgaagttgtg gatgaaaaat tttttatttg tttttttatt ataaaaaaac acttatttga
    14221 caaggatgta aacgactttt tggtttatca acaagtttat ccactattga caaatagatt
    14281 atccacaaac agtggaaaac ttgaggataa actcagacaa aataagaatg tatgaaaaag
    14341 gggttgtgga taaactgtgg aaacgaaacg atatgatgaa acagaattgc aagaggcagc
    14401 cgaagcactg aaagaaggag aacttgttgc ttttcctacc gaaacagtat acggattggg
    14461 cgccaacgct cttttaccaa acgctgtcaa gaaagtgttt tctgtaaaag ggagaccgca
    14521 agataatcca ttaatcgtcc atgtcgcctc ttttgaacaa gtaaaagagt atgtggacaa
    14581 ctttcatccg gcagcagaga agattgtgaa gaacttctgg cctggaccat taactttgat
    14641 atttaagatc aaaaaagaca ctttgccctc agttgtgacg ggaggattat ccacagctgc
    14701 atttcgtata ccggataaca aaaaaacact agaagtaatc gaactttccg gtgttccgct
    14761 tgtcggtcca agtgcaaata cgtcgggaaa accaagtcca acgactgcag atcatgtata
    14821 tcatgatcta caaggaaaaa ttaccgggat tattgatgat ggagctacgc gaattggtgt
    14881 agaatccact gttcttgatt taagtgatcc aacagccatg cctatgattt tacgaccagg
    14941 tgcagtaacg aaagaacaaa tcgaagcagt catcgaatct cctgttgcta ttgatcagca
    15001 tcttgtaaaa gaaaatgaaa caccaaaagc ccctgggatg aaatacaaac actactcacc
    15061 cgatacacgt gttctgatgg tcagagatgg cgattggtca actgcagttc aatgggcaaa
    15121 aaataagaag atccgtgcag gtgtcatagc aagccccgaa attgcggatc aagtgcggac
    15181 agatactgca gctgtttata tgtataacga caactcagta gaagcggcgg ctaaaggatt
    15241 gtttgcaggt ctaagaggat tagatgaacc aacacttgga ttagatttga tttttgtaca
    15301 agtgtatcca gaaactggat tggggaatgc ttacatgaat cgcttgaaaa aagcagcagg
    15361 acaaaattat tttgaaaaat gatcaattgg ttattgtcgc attcttttag tatgatacaa
    15421 tgctatcaag taaatcttct atcagtgaac gaaaaactaa gcgtaactaa tcgtaataga
    15481 agagatacga atatttggca atttgttttt gatgggaaaa ataaaattgt aggaaaataa
    15541 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    15601 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    15661 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    15721 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    15781 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    15841 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    15901 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    15961 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    16021 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    16081 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    16141 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    16201 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    16261 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    16321 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    16381 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    16441 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    16501 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    16561 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    16621 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    16681 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    16741 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    16801 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    16861 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    16921 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    16981 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    17041 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    17101 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    17161 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    17221 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    17281 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    17341 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    17401 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    17461 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    17521 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    17581 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    17641 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    17701 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    17761 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    17821 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    17881 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    17941 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    18001 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    18061 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    18121 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    18181 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    18241 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    18301 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    18361 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    18421 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    18481 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    18541 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    18601 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    18661 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    18721 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    18781 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    18841 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    18901 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    18961 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    19021 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    19081 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    19141 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    19201 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    19261 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    19321 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    19381 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    19441 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    19501 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    19561 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    19621 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    19681 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    19741 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    19801 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    19861 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    19921 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    19981 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    20041 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    20101 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    20161 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
    20221 tacttcaatc atttcaaagg agttgctact taaatcgata ttttttaaat cagtatcaaa
    20281 aaagctagca ttttctaaat cattagtaga aaacattatt ttagtgaggg aacagtctac
    20341 aaaatctgcg cttgataaga cagagtgctc aaaggtactt tcttttatgt ttgaaccaga
    20401 taaatttaca tagtccattt tacaattctc gaataacgtg ttcccaatat aactttccat
    20461 caatttagta cctgttaatt tacaattgac aaatttcacg cgatgcaaca tgctttttgt
    20521 caatatacag cctgaaaaat cacagttttc gaatatacag tcggtaatct ccattctttg
    20581 aaaatcattg gaagcaaaaa atgcatactt gaaaatagat ttgtaaataa ttggatggca
    20641 gattatttgg ttttcgatga cctctttttg tgaaaggata ttatacagtt caaaatcttc
    20701 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
    20761 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
    20821 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
    20881 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
    20941 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
    21001 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
    21061 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
    21121 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
    21181 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
    21241 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
    21301 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
    21361 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
    21421 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag gctaggttca
    21481 tttatttgaa cagagtctgt att
//
LOCUS       DACCXW010000041        21336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCXW010000041 DACCXW010000000
VERSION     DACCXW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.041"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            16..717
                     /locus_tag="IVY71_001272"
     CDS             16..717
                     /locus_tag="IVY71_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7985336.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1326..2342)
                     /gene="tsaD"
                     /locus_tag="IVY71_001273"
     CDS             complement(1326..2342)
                     /gene="tsaD"
                     /locus_tag="IVY71_001273"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7985337.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2339..2803)
                     /gene="rimI"
                     /locus_tag="IVY71_001274"
     CDS             complement(2339..2803)
                     /gene="rimI"
                     /locus_tag="IVY71_001274"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7985338.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2810..3352)
                     /gene="rimI"
                     /locus_tag="IVY71_001275"
     CDS             complement(2810..3352)
                     /gene="rimI"
                     /locus_tag="IVY71_001275"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7985339.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3336..4160)
                     /gene="tsaB"
                     /locus_tag="IVY71_001276"
     CDS             complement(3336..4160)
                     /gene="tsaB"
                     /locus_tag="IVY71_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7985340.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4249..5229)
                     /locus_tag="IVY71_001277"
     CDS             complement(4249..5229)
                     /locus_tag="IVY71_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7985341.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5253..6737)
                     /gene="galT"
                     /locus_tag="IVY71_001278"
     CDS             complement(5253..6737)
                     /gene="galT"
                     /locus_tag="IVY71_001278"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7985342.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6749..7738)
                     /gene="galE"
                     /locus_tag="IVY71_001279"
     CDS             complement(6749..7738)
                     /gene="galE"
                     /locus_tag="IVY71_001279"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7985343.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7986..8153
                     /locus_tag="IVY71_001280"
     CDS             7986..8153
                     /locus_tag="IVY71_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7985344.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8215..10026)
                     /locus_tag="IVY71_001281"
     CDS             complement(8215..10026)
                     /locus_tag="IVY71_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7985345.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10023..10388)
                     /locus_tag="IVY71_001282"
     CDS             complement(10023..10388)
                     /locus_tag="IVY71_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7985346.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10551..10946)
                     /locus_tag="IVY71_001283"
     CDS             complement(10551..10946)
                     /locus_tag="IVY71_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7985347.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10964..11926)
                     /locus_tag="IVY71_001284"
     CDS             complement(10964..11926)
                     /locus_tag="IVY71_001284"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7985348.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11926..12138)
                     /locus_tag="IVY71_001285"
     CDS             complement(11926..12138)
                     /locus_tag="IVY71_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7985349.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12159..12857)
                     /locus_tag="IVY71_001286"
     CDS             complement(12159..12857)
                     /locus_tag="IVY71_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7985350.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12877..13419)
                     /locus_tag="IVY71_001287"
     CDS             complement(12877..13419)
                     /locus_tag="IVY71_001287"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7985351.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13551..14555)
                     /locus_tag="IVY71_001288"
     CDS             complement(13551..14555)
                     /locus_tag="IVY71_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7985352.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14552..15541)
                     /locus_tag="IVY71_001289"
     CDS             complement(14552..15541)
                     /locus_tag="IVY71_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7985353.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15538..16344)
                     /locus_tag="IVY71_001290"
     CDS             complement(15538..16344)
                     /locus_tag="IVY71_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7985354.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16510..17466
                     /locus_tag="IVY71_001291"
     CDS             16510..17466
                     /locus_tag="IVY71_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7985355.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17543..18061)
                     /locus_tag="IVY71_001292"
     CDS             complement(17543..18061)
                     /locus_tag="IVY71_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7985356.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18149..18298)
                     /gene="rpmG"
                     /locus_tag="IVY71_001293"
     CDS             complement(18149..18298)
                     /gene="rpmG"
                     /locus_tag="IVY71_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7985357.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18526..18972
                     /locus_tag="IVY71_001294"
     CDS             18526..18972
                     /locus_tag="IVY71_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7985358.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19167..21062)
                     /gene="asnB"
                     /locus_tag="IVY71_001295"
     CDS             complement(19167..21062)
                     /gene="asnB"
                     /locus_tag="IVY71_001295"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7985359.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6403 a         4542 c         3688 g         6703 t
ORIGIN      
        1 ggagaggact ttttaatgcg tgttaataaa atcgattttc acttttcaaa actcagatat
       61 caacctgatt ttgaacattt ctcggatgaa gagttccaac agatcaagga acatactgtt
      121 cttcgttctt ataaaaaagg acagatcctt ttcgacgatg gtgatcccag acaacggttg
      181 tatatcttga ccaaaggact cgttcgttta gaacgttatg atgaaagtgc aacttattac
      241 tactatgact atgtgaatac aaataacctt tttcctatgt caggaatgtt tggagaagag
      301 acctacactt atacagctca agcgatgact gatattgaaa cattctacct tcctgtttcc
      361 tactatgaaa aattagcacg aaaaaatccc gaacagttga tctatatgat acaaaaaatt
      421 tctaaagtag ttgaaataca tgaattgcgt atccaaatcg gattgacctc tagcgccttt
      481 gaccgggtaa aacaaaattt attcattttg aaagaagaat tgggcgtacc aacaaaagat
      541 ggtcatatca ccattcctta tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg
      601 acacgtgaaa ctgctggaca agtaatcaaa caactacgtg attccgggct gttagattat
      661 gacaaaaagc gttttacttt ctgtaagaca gctactactc cagctatgag agaataacgc
      721 gaacaaaaat aagcagtgac cctgataaat atcagggtca ctgcttattt tcctccctaa
      781 aacttctgaa agatccggca gttttagatc actggacaag actcaaagat tttctatgtg
      841 tgtttcagtt atgtaatatt cactgtttgc aaaaaatact gttagattaa ctagatgaag
      901 cgtcttgtcc tcatccgatc tagattattc gatattcctc gacaataaac gattactcag
      961 tgagggcaat cagaggaacg gataatgata aataagctgt aaaaaatagc aaaataacaa
     1021 taatgattaa aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca gccctatttc
     1081 gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga
     1141 ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc
     1201 ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac
     1261 atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcagc tttttcacaa
     1321 cctctttatt cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt
     1381 cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt
     1441 cgaagagggg gaatcactag ttctacctca gggagtttag ctgccaaagc atcctgcaat
     1501 ccttcacgca acccttgatt ggccgctaca cctccagcta taatcagttg cttgactgga
     1561 taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag
     1621 ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa
     1681 ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc
     1741 gctcgcggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg
     1801 cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca
     1861 tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta
     1921 tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct
     1981 gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca
     2041 aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg
     2101 acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag
     2161 cataaggtga tttgttctac gtgatggcgg ctagctactt caggtactac gccaccaaaa
     2221 cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta
     2281 acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc
     2341 atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc
     2401 gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg
     2461 ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg
     2521 taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag
     2581 caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct
     2641 ggttatcttc agctaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact
     2701 gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt
     2761 ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt
     2821 ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga
     2881 tcgttgttgc ttgaaacccc aatttccgat aaagtctttg tgcatcttga ttactcatac
     2941 gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa
     3001 taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat
     3061 ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga
     3121 tacctagata tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc
     3181 atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg
     3241 tagtcaattc tcttagtacg taacgacgat cgatttgttc gactgttttt tctgtataaa
     3301 tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg
     3361 tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctactcgttt taaatagcga
     3421 ggaagaaaag cttgtttctc tgcaactggt tcaactaaag cacctaattg agccacagtt
     3481 acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc
     3541 tttatttcag agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc
     3601 ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacagtttct
     3661 aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac
     3721 atgggcacaa taatattatc cacaccgatg caattagccg cgatggtttt cagactagat
     3781 actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata
     3841 cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga
     3901 cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg
     3961 cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc
     4021 aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg
     4081 acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca
     4141 aaactagtac aataatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac
     4201 acaaaaaagc tgcggtatca ctccattttc ccacagcttt tccatctttt atttcgttga
     4261 ttctcgaata atcaactctg tttccacaat aatctttctt gaaaccggtg ctttggaatt
     4321 acttaattga agaatcgtct ccactgctag ctctcccatc cattcggtat aaacttttac
     4381 agtagaaagc gagggcgtta catatttcgc tacactgaca tcattgaagc ccatcacaga
     4441 aatatcttct ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa
     4501 tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta aatattgctt
     4561 cactgcttga tatcctgact cgacagaaaa atctgcttcg ataattggac cttcgtcgta
     4621 gcctttttct gccaaaactt gttgaaatgc cataattctg ggatctttta gccgctgatg
     4681 gtttttcttc gtatattctg ttccagcaag catagcgata tgttgcgctt tatgggagac
     4741 aaagtgttca atgacttttt gaacacttga cttaaagtcg acaactagag agtcgattcc
     4801 ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt tgggatcctt tcatttctaa
     4861 taactgagct tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa gatcactaat
     4921 ggaatcccac gtttctttca ataaagtaac gtttgcttct tctgcctttt tttcgattcc
     4981 taggcgaata gataaataat aaagatcttc aagctcttcc gtatcgtcgt accactggat
     5041 caggcgaagc gtttgcttag tatttgctgc atttttacga tgtttcgtat aatttaagcg
     5101 ttctgctgct tcaaaaattt tttgctttgt ggctaagcca acggaaagat caggatcata
     5161 gttgagaact cttgaaacag tagcaggaga aactcctgcc aactgcgcaa tgtctttaat
     5221 tgttgccatt tgtctctcct ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac
     5281 gctgcctgtc ctttttcatc tcgcttgaat actccagcgt cttccagtac acgtgcaaaa
     5341 acctgaccta cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt
     5401 tcttttttta attgttctgc ccacggacga tgatattcag ccatctggtt ttcttctcct
     5461 aataagaaat gctctacttc tactagttct ggtttcaaac gaggtggtaa aacagcaagc
     5521 cccattactt cgatcaaccc aatgttctcc tttttgatat gttggacatc tttatgtgga
     5581 tgaaaaatcc catcaggatg ttcttctgaa acattgttgt ctcgaaggac aaggtccaac
     5641 tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct
     5701 gctgaaaaag ctctgataga tactgtttca tccgaatatg ctttccattt ttccaatact
     5761 gagttagctg cttcaataag caagtttttc tctgttgatt gcaaacggat cacagacatc
     5821 ggccatttca cgactcctgc gatgacttgc ggataatctt ctagtttgat aagtttttct
     5881 atcggtgctt ttgccatagg aaattcatga cgtcctgcct gataatgatc atgagaaaga
     5941 atcgatcctc caacaattgg taagtcagca ttcgaaccaa caaagtaatg aggcaagact
     6001 tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt
     6061 tctgataaaa tgatcgaatg ttcattgtaa taagcataag gggaatattg gaatccccag
     6121 ctttcaccgt ctagattcat tcgaataatc cggtgattgg tgcgggctgg atagttcaag
     6181 cgacctctat atccttcgtt ttccatgcaa agcatgcagg taggatagtc tacttttacc
     6241 gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg gcttagataa attgattgtg
     6301 atttccagtt caccatactc cgactctgcc ggaaatttga tgttttttgc gatggctctt
     6361 gttttgatgt agtcattttt cttacttaaa ttaaagaaat actcagttgc ttcctctggg
     6421 cttttcgaat agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa
     6481 tccattaatt gcgcttcaaa aatctcttgt tctgctggca attctgaaag aacaccattt
     6541 tctcttgcct tagcgacaag ctgatccaat aagtctacag attcttcctt aactggtctt
     6601 acttcaatgc tttctagagc atcttctcct accatcccta aaagacgatt ctgtaaatat
     6661 aagcggtcca tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc
     6721 gtttgactga ttgacatatc tcattcccct attcttcata accatttgga tggctgacat
     6781 gccaatccca tgctgttttg ataatcgctt tgatatctgt gtattcaggt ttccagccca
     6841 gaatctctct cgctttttca cttgaagcga ctaatcggct aggatctcca gctcttcttg
     6901 gtgctatttc tgctggaatt tcttttcctg tcacttctct agctgcttct agcatttcct
     6961 tgactgagta tcctttgctg ctgcctaaat tgaagaagtt gctttcattt ccttctttca
     7021 agtattccaa agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca taatctctga
     7081 tacatgttcc atctggtgta tcataatcgt ctccataaac agccaaggct tttctttgac
     7141 caagagctac ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga tcttctccga
     7201 tagatgcatc tgcttttgct ccggctacat tgaagtagcg caaagcgaca tagcgcatgc
     7261 catatgcttg gtcacaccac ttcatcattt tttccatcat caatttactt tcaccatacg
     7321 gatttttagg attagctggt gtatcttctg tgattggaga ctctttcggc tctccataag
     7381 ttgctgcagt agaagagaat acgattttat tcacgttgaa ctcgtgcatg acttcaagta
     7441 agatttgcat accgtatacg ttgttgttga aatacatcaa tggtttttcg acagattctc
     7501 ctactaaaga gcttgcagca aaatggatta ctccttcaat cggttccttt tcaaatacgg
     7561 aacgtagaaa ttctttatcc cggatgtctc cttcataaaa atgagcatct ggatgtactg
     7621 cttgtttgtg acctgtgagc aaattatcta ccacagctac ttgatacccc ttttgaacta
     7681 gctggtctac agcgtgcgaa ccgatataac cggctccgcc tagtactaaa attgtcataa
     7741 aaaacactcc tttgattagt aaaattgtgt ttgttgattc tatataattt aaaaaacgaa
     7801 gtttactttt atggtgaaaa caaacaaagt aaaatttcgt aaattcatat ggtaaattta
     7861 taataaaaca tttagtaaac attttcaagg gttttttgca aaggtttaca aacaaacgta
     7921 aaactagcct ttttttctct tttgttccat aatagatata acaaataata aatgaggtgg
     7981 attcaatgaa aaaatttgta tctggaatat tagtaggaag tctggcaaca gccgctgccg
     8041 ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag
     8101 caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg cgtttctcgt taataattat
     8161 ttaaaaatac gcatgaagac tttctaaaaa acagataagt catcatgcgt atttttattt
     8221 gtcagtcaat ataagttgtg ctattttatc tgcctgtctc agattccatg gatctgtata
     8281 aggtctagct actgctgcag ttagccaatc cacaccttca acaggtattc catagcaata
     8341 aaagtcagac tggatttccc ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc
     8401 tatagcttct gaatgataac ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt
     8461 gttttccttc aaatttttta tgacaggatt acttgaacga gaaaagcttg tttgtggcaa
     8521 tcgagcttca atcaaatagc gactgctgat ttctttatct tgtagagaaa taacatagcg
     8581 gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat
     8641 cagtgctgct aattctttcg tccgaatgat tggcggaccg atcgttaaaa agctatttag
     8701 atgagtaaac catccccata gtatttcttt gtattcctta gcagaaaaaa cgtgccattc
     8761 aatagcctgc cggattggat cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc
     8821 tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct tcaataaata ttcggctagc
     8881 ttctgaaaag ggctcattct gataggcaag aggagtttct aaatacgacc aatcccatct
     8941 ataagggatt agtgctggat gctttccgag acactcctct gaagagaggg agagaaaatc
     9001 atgctcaaaa tttttctttg gaatcggcaa gtgatattct tcgatgattt tcttgtaata
     9061 aaacaactca acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga
     9121 ctgatgccat tgctccaggt tttcttttgt tagtaaccgt ggccatgcca ttgctccgcc
     9181 ttgcttttga tttcttcctc gagggtaata agggagcccc cttcttgatc cactgtatac
     9241 aatcggttcc ttgcctgatg gatgatagac cagttttccc ttttcgttct cgaagcgccc
     9301 tcctcttgat actgtaaaca atccgacata atcaaaaaaa cttaagccta gacctcttaa
     9361 aaagacattt tctttaggcg gtatcttcga aagatctaca tctgcaggat tagaaggaag
     9421 ctgatacagt aaaacatttt tctttgcgta gtctacccac actctttctt cttctgtctc
     9481 atcattttcc cagtgaccgc ttgctaaaac aagctgatcg atagaatagc tttgctgatc
     9541 tgtctgtaaa agataaccat cttttctttt gcttactttt tttactaaag aataaacaaa
     9601 ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc
     9661 gtataaacat cgagtagatt gctgatcttt ttccagacga ttgatttctt ctataattag
     9721 ttgcttattt ttggttaccg tctgttcgat ataatctgta gcaggtcctt tactccattg
     9781 aaacaagttg ggtccctgtg ttaccaatcc tccactggac aatgtttcat ctgtaaaaag
     9841 cgtcacttgt tgagaaacag aattcattaa taactccggt ggctgattca accgccaaac
     9901 tcgacctcca ggaccattgg gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt
     9961 attatggaga atcctttctg ctacacttaa tcctctaggt ccggcaccaa tgatccctat
    10021 tttcatttgc atgcctccat ttcgataaat tttttcaata aaacagcatg gttctgatgt
    10081 tcattttttg caccataaac aaaagtaatg actggatgtt ctttcataag ttgactgagt
    10141 ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt cttgctggta tttctttgca
    10201 aatgaagcaa atcgttctgg tacatggtca aaagttttgc gtgcttcatt actaggtgca
    10261 atttctttca accacagatc taactgcgct ttttcttttg aaattcctcg tggccagaat
    10321 ctatccacga gtattctata gccatcctct ttttcagcat caaggtaaac acgttttaat
    10381 ttgatcatag ttgtttcctt ctttctacct tttcgcttag cttatatatg tagataatag
    10441 cacaaaataa aaaaaatgaa cggcacaact atccattgcg ccgttcatgt gatcttggtc
    10501 aaaatctaat gattgattcg gtttttaaag ctgtcataca atcatctgtt ttatactttt
    10561 ttacttttga tttttccaga ccatccgtca tatccaccct taagtaaata aatctcttga
    10621 taacctgctt tacgtaattt atttgctgta cggatgctca aacttttctt ttgatcgtaa
    10681 atatagacag gctggtcttt tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg
    10741 atgtttcttg cacctaaaat atgcccagca tcaaacacat ctttttcgcg aacatcgatt
    10801 acttgtgctt ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt
    10861 ttggccatta ctcgtagata gacttcccaa aggatgattg caagcacaat caatagcaaa
    10921 atgatattaa ttacccataa aaccatgtaa tgcctccgtt tttttaagca aatttcaagg
    10981 ctaatgaggc agcaccaatg acaccagctt cattaccaag ctctgctaat ttgatttttg
    11041 ttgattctct tacttgcggg aaagtaaatt ctttaaaata tttctctaca cggctacgta
    11101 aaaattctcc tgctgccgat actcctccac ctaacacgat acttgacgga tttaatgtat
    11161 tccccaagtt tccgcaagct aaacctaagt agaagcatac gcgatcaaca accatcaatg
    11221 caaacggatc gtcttcttga gccatttcaa agatatcttt acttgttact tcttctccgt
    11281 tgtctagcat tgctttcagt tttgaatccc ctgcgtattc ttctgccaag aaacgaccta
    11341 ggcgtaccac acctgtagcg ctagatacag tttctaaaca tccgcgttta ccgcaagtac
    11401 attcgaaccc ttcaggatcc acagtgatat gtccgatttc tcctccagct ccgctcacgc
    11461 cgtgaatcaa atttccgcca gcaacgatac ctccgccaac acctgttcct agagtaacga
    11521 atacaacgtc tggatcgttt tctccagcac ctttccatcg ttcacctaaa gcagcaacat
    11581 tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc ctcgatcagt tttttcacaa
    11641 attgaacttc tgtccagttt aagttatacg caccaataac cgttccagct tcgctgtcca
    11701 cgcttcctgg ggtacccatg ccgattccga taaagtcttc ggctttcata ttatataaat
    11761 tcaaacgatg attgattgat tcaatgattt caggaacgat atgcttacca tcatccaaaa
    11821 tattggtatc gatgctccat ttttgctgaa tctctccttc tggtgttaaa attgcaaatt
    11881 ttgctgttgt tccacctaaa tcaatcccaa taatcttctt gtccattaca aatttccttt
    11941 ctttcggctt tcttcaattc ggtgctctct ttttaatatg cgccacgctg tcatgtatgt
    12001 atcatgatca atcaaacggc tatcatacag attacgcaat tcgatagtca ttaattcaat
    12061 gtcatagatg cgtgcaccca catagataaa gatgccaaat tttttgaaca gctgctgtac
    12121 atcatataag ctctccatct ttaagcactt ccttcatatt atacaagtaa accatatttt
    12181 tttaaaccca gtaaaataca tattacaaaa agaaatacaa aaactagtgt agaaagaatc
    12241 ctttggtgta tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt ccctaacact
    12301 aatccgccaa ttagcccacc gacatgtccc caaacatcta cagaggtatc cagaagacca
    12361 aaaacaaatg tcaacacaat aaataaaaga aaatgtctga ctagctgctg gatcgctggg
    12421 ttatgtttaa aatgaaaccc taagataaac aacgcaccga acaaaccgaa tatcgaagta
    12481 cttgcaccac cagacagaac attcgcttcg ttaaaagcaa agcttgctgt atttcccatt
    12541 atcccgctga acatataaat caagaaaaaa cgccaatggc catagattgc ctcgatttgc
    12601 tgccccataa aatacagtat cagtgagttc attacgaaat gcatcagtcc aaaatggatg
    12661 aagattggtg tcactaaacg ccaccattca ttgaagtgaa caagataagg accatacatt
    12721 ccggcataat aagggatctc aagtcgcgga aataattcca tcatgaggaa gaccgctgtc
    12781 tgtatcccta aaaaagcata ggtccataac ggtcgtctta accatgcacg caattttatt
    12841 tgctgttgat aattcatgaa aacctcccat ttcattttag tcaataaaca attgttggat
    12901 cttctgatca aagctctccg gttgccattg ttcattcaat tgttcacgaa aaacaaggct
    12961 acatgttttt ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc caaaaccgat
    13021 tcgataaccg gctctattgt agataatacc tggtacaatg aggagatcga tcatctccgg
    13081 caaatagagc ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta caagaacgga
    13141 ctgttcatcc atttcataaa acgccatctg acgtttcggc aaagttttag gtacaactat
    13201 tttcttttgt tctttttctg ctcgttggat aaccggctgt gtatccagtt caaattcatt
    13261 cgataaagtc gtccctatca cttttgctga cttccagtac tcgctggcga atagctgttt
    13321 atagattcgc tgttcttttt cctttttctt gccacttttg gccagatttt tcagcttttc
    13381 gattcctttc cggcgtaatc ttactttcaa ttggtccaaa aactcaccct tccttctctc
    13441 ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa atgcataaat
    13501 agaataaaaa gaagacctag aaatgattgg ccttcctttt ttaatgaatt tcaactgttc
    13561 agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat atggcgcacc aaaaagggca
    13621 atcaaaattc ctgtaggtac ttccccactt ggtagaatca ctctagcaac catatctgca
    13681 ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt attttcttta
    13741 cgtacgacta gcttcgcagc atgaggagct agtagtccta caaaagataa actacctgtc
    13801 accgctacac taccgcttgc caaagcaaca gctaacaaca aaaggatttt ttgactttta
    13861 gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc
    13921 tgtgcataaa gtcctcctag cggaattaag ataaccgccc atggaagcaa tgcccaaaca
    13981 taaggccagc tcgcacccca gattgatccc gctaaccatg cattcacaaa tccataattt
    14041 tccttagata attggatcgt gcatagcaat gttaatgcag aaaggccggc attaacggca
    14101 ataccggaca ataagattcg attcataccc aaaaagccat ttcgatcata ggccgcaaga
    14161 tagacacagc aagcagctaa aacaccacct agaaaggcta caactagcaa tagaaacggc
    14221 agcgaatagt gcgaaaagaa acctaaataa agcatcacta aaaatcctgc tccggcatta
    14281 atgcccaata tactagaatc tgccaattca tttcttgtta ctccttgcaa gatataacca
    14341 gaaacgccaa gacaagctcc tgctaataaa ctgatcagta atcgaggaac tcgaaattct
    14401 ctaacaatca gcgccaaaga agatgattct ttccctatga acgtggcaaa aagctgatcc
    14461 aggtaaataa aaggcgtacc aatcattaaa ctacctatag acaggatgac aagtacaaaa
    14521 agcaagaaat agaacgtcca atatattttt ttcaccgttg ttccctcctt acttgtatca
    14581 gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa aggtgtttca aaagggggat
    14641 tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc accgccaact aaaaaagaga
    14701 caggtagaac ataacgatag tccgttccaa ccattccgcg aacaactggc ggaaccaaca
    14761 gcccaataaa tgaaacaggt ccgactaacg aaacggcaat ggctgctaac actgcaacta
    14821 gacagaaaac aatcaaccgc gttcgtttag gatgacttcc aagagctaat gcatgtgttt
    14881 cactcaaatg aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa acagcaagta
    14941 tatataaagg aaacaatact gctaattgtt tccatgtgat attggcagct ccgcctacaa
    15001 accaaaaagc aagatcctgc tgcagattaa agaattgggt aagtgcttgg ctgatagcag
    15061 taaaaaaaga actaatagct acgccagcta aaatcagctg taaaggattc atgcccattt
    15121 tcgaaaactg caaaaaacca aatacgagca aagttacagc aaaagaacct gccaatgaca
    15181 ttagaaacac agaaaaatca gaagctttag gccaaaaagc aaaactaagg gccattgcca
    15241 acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc taaaggattt cttgtcactc
    15301 cttgcatcaa tgcaccgctc aatgcaaaac aactccctac aagaaatgct cccgccacac
    15361 gaggcaaacg aatatttcgt atgatttggt gactttgttt tgatctatca aaatcaaaca
    15421 acgcctgcca aaaatctgct ttttccacag tcgctgcgcc atttgaaaga gcaacaaaaa
    15481 aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct atttttcttc atagtcctca
    15541 taattgatcc tcctgctctt ttggcaacaa atcataggtt agaaaaactg gtttatttgt
    15601 tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac actcttttgt
    15661 gatcacttct tcaggtgtac cttctgtgat caacttaccg tttttcattg ctaataaata
    15721 atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac atcaagatcg ttgtttgttt
    15781 catttgattg atttcagtca ataactgtag gatctctaat tggtgtgccg gatctaaaaa
    15841 tgttgtcggt tcatccaaga tcaagatatc tgtttcctga gcaagcgcca tagcgatcca
    15901 gacacgctgg gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa atccagctag
    15961 acctgtagca tccaacgccc actcaattgc ttcatgatct tgcgtgtcca tgccactgaa
    16021 cagtttttga taagggtatc gtccgtaaga tactacttct tctactgtca tacctgtagg
    16081 gtgttcacta gtttgtgcta ataatgcaat tttttttgca agttcttttg aactgtatgt
    16141 agagatatca gagttattga caatgatctt tcctgatttc acaggtagta tacgggtaaa
    16201 acttttcaat aaagtagatt ttccacttcc atttggccca atcaaactag tgattttccc
    16261 ttcaggaatc gtaacagaca tgttttcaat gatttttttg ttttcgtagc cggctgcaat
    16321 gtcagctgct cttaatctcg tcatttcacc catcctttcg ctgaaaatca ttctcaatta
    16381 tgacgctgtt tcaacatttc gtcaagcgct actttcatat ctatagccaa atgaagcggt
    16441 tgacagcgtt cgaaatatca ggaataataa ccaatgagaa agattctcaa ttatgttagg
    16501 aggaaagaaa tgaaattaaa aacaaaagta ttcacattat ctagcatatt gctgctggct
    16561 ggagtactga gcgcttgctc cccctcttca gataaagaaa cagctacaac tgccaattcg
    16621 acgacttcat ccaatagtac tcatacagtc acagatacac ttggacataa agtagagatt
    16681 ccaaacgcgc ccaaacggat catcggcagt tacttagaag actatctgat tgcactaggg
    16741 gaaaaaccta tcgcccagtg gactgttggc agtggttcca ttcaagatta tctgcaagat
    16801 gatttaaagg atgtcccaac gatttcttat gaccttcctt atgagaaagt gttaagcttt
    16861 gagcctgatt tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa
    16921 tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac atgggaagaa
    16981 caactaaaag atgtcggcaa agctttggca aaagaaaaag aggcagaaaa aatcatttcc
    17041 gattatcaaa aatccatcaa gaaaactcga gaagaactag cagataaaat cgagaacaag
    17101 acagctgcag tgttgtgggt gaccaacaac tcagccttta tggtcagcaa aaatcggtca
    17161 agtggaagaa tcgtatacga cgatctggaa tttggtgttc ctgatctagt ggaagaggtc
    17221 tccaaaaaag ccacttctga ctggtctgct gtttcctctg aaaagctagc tgaattagat
    17281 gctgactaca tcatccttgt gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc
    17341 tggaagaatc taaaagcagt gacagacaat catgtactgc aattcggacc tgaatctagt
    17401 tggctatata acggacctat tgccagtcag gaaatggtca atgatatcaa agaaaaacta
    17461 aattgatttt aactaaaaaa agcatcttca cgattagtcg tgagatgctt tttttcttat
    17521 taacaaacta gacgcataga ttttattctt cttttgaagc aaccctattt tttactcttt
    17581 cccatttttg aaaacgatgg gcatatagat tttcttgatc cgttatcccc tcatactcct
    17641 ctataagggc gaagtctgaa aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc
    17701 tatcgatcaa tgttcgccag atccgaccag tgtaaggaag aagttcttga aaaatccgac
    17761 taccacccga aacatagata tcttctgaga tcgtttgact atacgacaaa agttcatcga
    17821 tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga
    17881 tgatatggcg ataaggaagt gaaagatccc ccattccttc ataggtcttt cttcccatca
    17941 ctaaaattcg atccattgta tgttcgcgaa agaaacgcat atcatttggc aatcgccatg
    18001 gtaacaaacc atttttgccg atcagaccat ttttatcttg tgcccacatg gaaataaaca
    18061 tattatccgc tccttatgat tttagatgtt ttgagataaa aaaagtctca attctttgac
    18121 cagcaaggaa ttgagaactt taaaaacatt attttgtttc acggtgcaaa gtaactttgc
    18181 gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg attgttacgt ttgtttttgc
    18241 ttgttagata gttacgttct ttacaagaag tacactctaa agtgatgttt acgcgcatgt
    18301 ttttccctcc catttttaca agaatcctct aagatgactc tcagacttga ttatcttacc
    18361 atgatttttt ctagattgct agtgtttttc acgattttgt taagtaaaac tacttgacga
    18421 taaatacaac ggttttactt ctcaaacaaa atctttttct ttttgtataa atacccttac
    18481 acttgtttat atagattatg ttttttaata aagggggata ctaatatgcc atggaacatg
    18541 aaggactttc ctgcatcaat gaaaaactta gataaattga caagaaaaaa agcaatcgac
    18601 atcgcaaatg cattattaga tgaagggtac cctgataacc gtgcgatccc aattgccatt
    18661 gatcaagcaa aaaaatggta tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa
    18721 gaaaaaaatc caagcaagac ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt
    18781 gattcagatg tgatcgtaga atatgaggaa gaacaatgga tcgtgaaatc taaaggggca
    18841 aaaaaggcaa gcaaccattt tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta
    18901 gcacaaaaca aagaatcaac tttgttcatt tataaaaaag atgggacaaa agaaaaagaa
    18961 atcgattatt gaacatatgt aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg
    19021 ttcgaaacaa tggtgttcct ctgccaactc cgaaccataa gtcataatat ctataaaacg
    19081 ggaagagctg ttttttgaaa ttgtttctta tcactcagtg ttaaacggta ggagaaaacc
    19141 tcttttttat tgatatttga ttttcatcaa acagtcacat agtcgattgc ttctgcttgc
    19201 ggaatcgttt catctacaaa gtagacttta taccatgtca agaaactgaa gattgtccag
    19261 atttttcttt gctcttcttt acgtccttcg tgatggtcat ctagcaactt catgattttt
    19321 tcttgatcaa aaaattcttt tgcaaaatct gcttcgaata atgctttgac ttgttcgtag
    19381 ccgtgttctt ctttcaacca agctttgatt ggcacaggga atcctaattt cacacgattc
    19441 gaccattctt ccggaagatg tttgttcgca gcttgtctga aaatatcttt tgtgttgttt
    19501 tgattcaata aaaacttact tggtatttgt tgagccaatt tcatgacttc gatatccaat
    19561 aaaggtactc ggacttccaa agaacttgcc atagaaagtt tatctgcctt taacaagata
    19621 tcttttggca tccattgatg aagatcgaca tattgcatct tattgacttc atctttgatt
    19681 ccttctgttt tctcgtaatg aacagtcatg atttcatcca cagatggtgc tgtttgatat
    19741 tttggtgtta acacttctga tgcttctgat tcttcgaaca ctcgagcatg accgataaaa
    19801 tattcttttg caggagcaag tgattcatat aaatggatcc gtccatggaa gtttttcatt
    19861 tttccgattt tacgtcctaa attatagcgt gttccttttg gtaatttttt caatccttga
    19921 gcaatcacac ggatgaattt tgaattcgta tggaatccat acgtttggta tcccgcaaac
    19981 agttcatccg ccccttctcc agattggact acccgcacac tttgtgaagc aagatttgct
    20041 aaaaagtaca aaggtacgca agaaggattt gaatcaggtt catctaagtg atactggatc
    20101 aaagggaatg ctttgaacga catgtctccg tcgatcactg ctgctgtatt atctaagtcc
    20161 aatagttcgg ttagttttct ggcttcaatc gcttcattat aggttttatc atcaaacccg
    20221 attgagaaag aatgatctgg tttcaataca gaggttacat aactggaatc aaccccgctg
    20281 gataagaaag aaccaacttc tacatcactg accgtatgtg cttcaataga agcttgaacg
    20341 gtttcgtcga ttttttcgat ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct
    20401 gcatcccaat attgctgaat gtctattttt ccatctttat acgtataata atggccttca
    20461 ggcaaacgat agacatcttt gaagaatgtt tcgccgttta atggagaata ttggaaagtc
    20521 atataaggtt tcaacgcttc tttgttcaat tctttgttaa aatcaggatg aggcagaaaa
    20581 cttttgattt ctgaaccaaa cataaatgtt ccgttcattt ctgcataata atatggtttg
    20641 atcccaaagt ggtctcttgc accgaataac tcattctttt cattatccca aatagcaaaa
    20701 gcaaacattc cgcggatctt ttgcaataat tctgttcccc attcttcgta accatgcaaa
    20761 aggacttctg tatctgcatg tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc
    20821 aaaggctggt aattatagat ttcgccatta aaaataatga tctttgtccg atcttcattg
    20881 taaataggct gagtaccgcc ctctaagtca ataatactta atcttctgaa tcctaaagca
    20941 acatgtttat cgatatattc tcctgaacta ttaggtccac ggtgaacgat tcgatccatc
    21001 attgtattaa taattgtttt tttgttgtcc ttgtcgttaa caaaacctac aataccgcac
    21061 atttaacttt cacatcctaa tataaaatta ctgtttattt cattcatatg catcttatca
    21121 tagaataaat agcggacgaa tattttctct cttacaattt cgtcacgtga aaacaaaaga
    21181 tgaaagtttc tgtttttaca gaaaataaag tcccaatatc taatttacca tgttttcttt
    21241 tttgaggcta ccttattctc ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa
    21301 aaccagcaaa aaaagacacg acctaaagat cgtgtc
//
LOCUS       DACCXW010000042        21102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCXW010000042 DACCXW010000000
VERSION     DACCXW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.042"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(122..499)
                     /locus_tag="IVY71_001296"
     CDS             complement(122..499)
                     /locus_tag="IVY71_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985360.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="IVY71_001297"
     CDS             755..1483
                     /locus_tag="IVY71_001297"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7985361.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="IVY71_001298"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="IVY71_001298"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7985362.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="IVY71_001299"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="IVY71_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7985363.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="IVY71_001300"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="IVY71_001300"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7985364.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="IVY71_001301"
     CDS             4618..6057
                     /locus_tag="IVY71_001301"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7985365.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6089..7132
                     /locus_tag="IVY71_001302"
     CDS             6089..7132
                     /locus_tag="IVY71_001302"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7985366.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="IVY71_001303"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="IVY71_001303"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7985367.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="IVY71_001304"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="IVY71_001304"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7985368.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="IVY71_001305"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="IVY71_001305"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7985369.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="IVY71_001306"
     CDS             10651..11565
                     /locus_tag="IVY71_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7985370.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="IVY71_001307"
     CDS             complement(11698..13263)
                     /locus_tag="IVY71_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7985371.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="IVY71_001308"
     CDS             complement(13256..14707)
                     /locus_tag="IVY71_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7985372.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="IVY71_001309"
     CDS             complement(14721..15737)
                     /locus_tag="IVY71_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7985373.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="IVY71_001310"
     CDS             15957..17045
                     /locus_tag="IVY71_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7985374.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="IVY71_001311"
     CDS             17099..18625
                     /locus_tag="IVY71_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7985375.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="IVY71_001312"
     CDS             18627..19832
                     /locus_tag="IVY71_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7985376.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="IVY71_001313"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="IVY71_001313"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7985377.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6757 a         3577 c         4797 g         5971 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag tt
//
LOCUS       DACCXW010000043        21086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCXW010000043 DACCXW010000000
VERSION     DACCXW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.043"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            12..302
                     /locus_tag="IVY71_001314"
     CDS             12..302
                     /locus_tag="IVY71_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985378.1"
                     /translation="MLDMRIEDYRITSDSRNIVLSKVRRDEEGNIRYTEAKEESRADI
                     GYFQTVSSCLKAIQRDYVLSEERTIKSIIEYKKALENITRQFEQACEIEEEK"
     gene            302..619
                     /locus_tag="IVY71_001315"
     CDS             302..619
                     /locus_tag="IVY71_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985379.1"
                     /translation="MEFVDLNVRGIKCDNPECDYSDMAVKYENYPQWLNKPCPKCGAN
                     LLTQEDLDATEQLMEIVNLTNEILKDSGLEKQDMNKYIVPVEANGTGELSFGEIKKLE
                     EEK"
     gene            616..1431
                     /locus_tag="IVY71_001316"
     CDS             616..1431
                     /locus_tag="IVY71_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP7985380.1"
                     /translation="MNENKLIKLGVAGVVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKEHGWVTVNGADTVVTDETGK"
     gene            1570..1884
                     /locus_tag="IVY71_001317"
     CDS             1570..1884
                     /locus_tag="IVY71_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7985381.1"
                     /translation="MDLITQYSEIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            2061..2372
                     /locus_tag="IVY71_001318"
     CDS             2061..2372
                     /locus_tag="IVY71_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985382.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEEA
                     E"
     gene            2369..2572
                     /locus_tag="IVY71_001319"
     CDS             2369..2572
                     /locus_tag="IVY71_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7985383.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            2569..2865
                     /locus_tag="IVY71_001320"
     CDS             2569..2865
                     /locus_tag="IVY71_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985384.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            2942..3358
                     /locus_tag="IVY71_001321"
     CDS             2942..3358
                     /locus_tag="IVY71_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7985385.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            4264..4608
                     /locus_tag="IVY71_001322"
     CDS             4264..4608
                     /locus_tag="IVY71_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7985386.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            4613..4894
                     /locus_tag="IVY71_001323"
     CDS             4613..4894
                     /locus_tag="IVY71_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985387.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            4998..5312
                     /locus_tag="IVY71_001324"
     CDS             4998..5312
                     /locus_tag="IVY71_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7985388.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            5290..6984
                     /locus_tag="IVY71_001325"
     CDS             5290..6984
                     /locus_tag="IVY71_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7985389.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            7004..8182
                     /locus_tag="IVY71_001326"
     CDS             7004..8182
                     /locus_tag="IVY71_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7985390.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            8145..8831
                     /locus_tag="IVY71_001327"
     CDS             8145..8831
                     /locus_tag="IVY71_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7985391.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            8831..9991
                     /locus_tag="IVY71_001328"
     CDS             8831..9991
                     /locus_tag="IVY71_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7985392.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKEAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            10001..10876
                     /locus_tag="IVY71_001329"
     CDS             10001..10876
                     /locus_tag="IVY71_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985393.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            10873..11184
                     /locus_tag="IVY71_001330"
     CDS             10873..11184
                     /locus_tag="IVY71_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985394.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            11174..11527
                     /locus_tag="IVY71_001331"
     CDS             11174..11527
                     /locus_tag="IVY71_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7985395.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            11517..11918
                     /locus_tag="IVY71_001332"
     CDS             11517..11918
                     /locus_tag="IVY71_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985396.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            11911..12315
                     /locus_tag="IVY71_001333"
     CDS             11911..12315
                     /locus_tag="IVY71_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985397.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            12327..12932
                     /locus_tag="IVY71_001334"
     CDS             12327..12932
                     /locus_tag="IVY71_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7985398.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            12952..13314
                     /locus_tag="IVY71_001335"
     CDS             12952..13314
                     /locus_tag="IVY71_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985399.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            13317..13499
                     /locus_tag="IVY71_001336"
     CDS             13317..13499
                     /locus_tag="IVY71_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985400.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            13516..16935
                     /locus_tag="IVY71_001337"
     CDS             13516..16935
                     /locus_tag="IVY71_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7985401.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWIQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            16985..17722
                     /locus_tag="IVY71_001338"
     CDS             16985..17722
                     /locus_tag="IVY71_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7985402.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDAEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFSLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            17732..20023
                     /locus_tag="IVY71_001339"
     CDS             17732..20023
                     /locus_tag="IVY71_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985403.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNMIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHETRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVEDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVNNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQDGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKIA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            20047..20925
                     /locus_tag="IVY71_001340"
                     /pseudo
     CDS             20047..20925
                     /locus_tag="IVY71_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7481 a         3090 c         4569 g         5946 t
ORIGIN      
        1 gaggagaaaa aatgctagac atgagaatcg aagattatcg aattaccagt gattctagaa
       61 acattgtcct atcaaaggta agacgagatg aggaaggaaa tatccgctac acagaagcaa
      121 aagaagaatc acgagcagat atcggatact tccaaactgt ctcatcgtgt ttgaaggcga
      181 tacaacgcga ttacgtgtta agtgaagaaa gaacgataaa aagtattatc gagtacaaaa
      241 aagcgttaga aaacatcact agacagtttg aacaggcatg tgagattgag gaggagaaat
      301 aatggaattc gtagacttaa atgtacgtgg gatcaaatgc gataatccag aatgtgatta
      361 ttctgatatg gcagtaaaat acgaaaatta tccgcaatgg ttgaacaagc catgtccgaa
      421 atgcggcgct aatcttctga cgcaagaaga cttggatgca acagagcagc ttatggagat
      481 tgtcaatctt acaaacgaga tcctcaaaga ttcaggattg gagaagcagg atatgaataa
      541 atatatcgta cctgtagaag cgaatggaac cggtgaattg tcatttggag aaataaaaaa
      601 actggaggaa gaaaaatgaa cgaaaataaa ttaatcaaac taggtgtggc aggagtagta
      661 atagtaggta ttggagttat cggaggattt aagtttttcg aaaaaattga taacggatat
      721 gtgggtgtgc gctattcaat gaatggcggt atcaaagatg aagcactgac gcaaggtgtg
      781 aaatttgttg ggattgacaa agtgatccaa tatccaattc gcttgcaaac tatccaatca
      841 aaaaacattt cagtatcaac aagcgatggc aaaaagacaa cgattgatat caaatatgac
      901 tacaaagttg attcaactaa agcagcaaaa atgtacaaag aatttgggaa tataacttcg
      961 gaagatatcg aaagtggatg gttaaaatct aagcttcaaa aagtagcgcg tgaagtttat
     1021 gccaaatata gcctgcttga tgtcctttca ggagattcct ctaaagttga agctgaggta
     1081 ctaacgaact ttgctaaatc agttgaatct aaagggtttg aagtcgaaga cgtaacactt
     1141 ggtgttccag atgtcgataa agaaacacaa aaatcaattg atgcgatcat tcgagctggt
     1201 caagaaaatg aaaaagcgaa gctagatgca gaaactgcaa aaactcaagc tgatagtgaa
     1261 gcttacaaga aaacaaaagc tgcagaagcg gaagctgaat caaaccgtaa agttgccgaa
     1321 tctgtaacag ataatttaat tcgttatgaa gaagctcaag ctcgaaaaga gcatggatgg
     1381 gtaacagtaa acggagcaga tactgtagtt acggatgaaa caggtaaata atatgggatt
     1441 ctttatagct aaaattctct tgttcttagg tttggtagga gtggcatatc tcgtgtatgc
     1501 cctcctttcc aaaactgatg acaaagaaga tgacaacgac gatgaaatga aattttagga
     1561 ggagaaataa tggatctcat tacacaatac agtgaaatca tcctcaagaa aatcatgatg
     1621 aagattcaga aagataaaaa atcaaaagaa cgagcggaat tagttaagtt ggaaatggct
     1681 gaaacaggag caggagtgcg aagtagcagg cattggaaag cagcagcaaa cattgaattc
     1741 tattacaacg aaattcaaaa agggttcgat cagatgcgtg agctggatcg gcaaacaaat
     1801 tggagcaaga aacttcatca agatcgtttc aaatttgtag aaaagtatag agagatactg
     1861 aatgagtatt tcgaggagga ctagatgttt aatttaatgt tagcgtcaat tcacttttta
     1921 ttctatataa cgatatttgt agctatatta attaatacga taaaaagcgg ttttttaaca
     1981 ccaatcggca caattttaac attagcttca tttgttacaa gttgtattat ccaacttaaa
     2041 tatataaagg agaatgaata atggacgaac taatcacaaa agtagagcag tgggctaaag
     2101 ataagggatt ggatcaagct gattccagca agcaaatgtt aaaaacgatc gaagagattg
     2161 gggaagttgc cgcttctcta gctagaaaag atgaacatgg tttaagagac ggaattggag
     2221 acgtagtagt aaccttgatt attttagcta tgcaaaatga tatggatttg tacgagtgtc
     2281 tgaaccaagc atacaacgaa atcaaaggac gcacagggaa aatggtagat ggtgtattcg
     2341 tgaagtcgag tgatttggag gaagcggaat gaacaacaga catcgcagaa taacaaaact
     2401 aagaaaacag gaactgaatg tactaaagac aaagtttgaa aaagaatatg gaatttcagc
     2461 agaagaaaca tataaagtgg caagtcagtg tgttgctgat gcaagtgatg ctattcgtaa
     2521 gtttggaatt tcgatattaa gtgatgatcg taaatgggag gaaaaagaat gaaactaaaa
     2581 gacggatttt acgctagcag tcacggtatc ggcggtttaa tgctagatat gccgacaaag
     2641 aatcctaaaa cacgtaagaa accaaaagtc aaagtcggtg acatggtccg ctgtgaagca
     2701 gaggagttca tttatccatt tcgtggatat gtagaacacg tctataatca ctcagcgatc
     2761 attcgcattg aaaacacgat ggaatgtgat aaatggacag ctaaaagcaa agagaattta
     2821 gcagtagtga gattggtgga tatggaacta atcaatgaca aataaaaaag ccggatcgct
     2881 ccgactaaca taataaaaca gacaagttta ttatatcaca taaaaggagc agtttgactt
     2941 gatgcaattg ttacgagagg tagatttcaa acagacaaga tgtaatgcga gagatgtgct
     3001 gaagaacttt cggcgtttgg agcggatggc aggtcgctct ttgatagata ttaagtcgcc
     3061 gattattacg gatatgccga aggcaccgaa gcacggcaat aaggcagagg atgcattgat
     3121 tcagatgatg gatatagaag cggagagaga cgcgatttta gcggctttga tggctcttag
     3181 tctgattagt cgtcagatac tctactatag cttctgtgac gtaaataagc actctaatta
     3241 tgaaataggt caattgatac gaggatacgg agagaagaat gtagagaagc tgaaatctat
     3301 cgcgctgatc gaatttgctg aagcatacaa aaaaggcgtg ttagttcagt atcgttgatt
     3361 ttgtagggtt tttgtaggga tagtgtaggg tttttgagtg ttttaacgtg atattatgat
     3421 agtgtcgaaa gattaggaaa caggatcgac aaaataaaat gtaagggagg aaatctccct
     3481 catcgtttaa ttaagcttcg atagacagca gcggaaatat taagaataag gatgtgaatt
     3541 ttaactcctt ctaaattgtt cttattatct atcatccgtt gctgtctatt gtcattatgt
     3601 cactgtggcg gaaagggtag acgcttaaaa ataaggtcaa tacgtcgagg gatagcctta
     3661 acgttttatg atttgaccat gcaatgttcg attcattgcc agtgacttaa ggaacctacg
     3721 gaaacaattc atcttatcgg atgccgatga attggctgac tagtcgggat gctactagca
     3781 gttagaaggc ataaaatact agcgcagacg tgcgccactc tcaggtgtag gttaggagag
     3841 aaacattagt tggggttatt aggaatacga taacctgctt gcgacaaagc tttgtactgt
     3901 cgcgttggtc atgaacagag acggtattct gtttcagtat tcgttagcaa ccgagggatg
     3961 tggcagtggt gaggtgcagg aagtatcaga cttgtttgtg tgcaagttac tattacatat
     4021 tagatcactc tttgagtggt ctttttattt tgcgtaaagg aggcacgacg atgtatagac
     4081 cacaatactt agaacagaag catgaagtaa tcactgtgca aaacggtaac ggtgagatag
     4141 tacgaaagta tagaagacca ataaagagcg atacatataa acggaaggaa agcaatgaag
     4201 ttattccatt gtatggcaaa agaatagcta agtattaaat aagattgcga aaggagatgg
     4261 gacatgaccg aggaattcta tagatggtta ttacagttga taagagaaga tcgtttggtt
     4321 aagttctatc agtctcctaa atggcgcagg cttagagaga aagcgatgaa acgagatcac
     4381 tatgaatgcc aagagtgtag aagactaggt aagtatcata gagtagagaa cgttcatcat
     4441 ataaaggaag tcaaggatag acctgactta gctttagatt tagataatct tatttgttta
     4501 tgtgttgaac atcataatga agttcatggc agatatctta cagcgttaga taaacaagag
     4561 aagaagatag aaagcttcgc taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat
     4621 caatgacaat ggcagagagt atgatacaga aaagattgaa gagtattcat cttatactca
     4681 gggattaatt aaacgtttga tatacgttcg ctatgtaggt attagggatc tgttatcaga
     4741 taactgttgt agtaaataca aagtgaatca agtaagagaa gcgttgaata aagataataa
     4801 cgtcgaaaga ataaaaaatg tttttggata tagtattgaa gagattaatt attacattga
     4861 cttcgctgaa gctttcattc cgatggtgag ataacccccc ccttaaaata aatcgcaaat
     4921 tttttggggg tgatgaaacg gagggggctg tcaggaaaag agattttttc gaactttatc
     4981 atgaaaggag ggctaaaatg tttaaaaacg aattgtctca aaatcgctac agagaaaaat
     5041 tacgccgctc tttaataagc caattggaaa gtcagaaaac aaatattgag ccattcttag
     5101 ataatgttga tcgttatatc agtttatggg aaacggcgat atcactggaa gaagatatat
     5161 ccgagaacgg cattagactg gagaatggta aaaagaatga atcagtagcg ttgcttgttt
     5221 ctgtcaacaa acaaatggga ttgatgttgg ataaacttgc cattactcct gaattggtag
     5281 gtgaagcaaa tgaatcaatt cctgagttat aagcatattg aaaattggtt caaagctata
     5341 gaagaaggca ctatcaaggt atgcaaagag caattattgc taaaaaatta tctagaagaa
     5401 agagtcttta ctagagaaga tatttacttc gataagcaga tggtagagga ttcaatcaat
     5461 ataccagcac aatactttcc attcgaatta attccgtggg aaaaatttct acaatgtttt
     5521 atttatggtg ttcgatggaa aaaagataaa acactagtgt tcaatagata tctttcatta
     5581 atgggacgtg gtaatggtaa aactggtttt gcttcttgga acaacttctt tctactaacc
     5641 gctaaacacg gtattaaaaa ttatgatatt gatatctatg ccaataatga aagccaagca
     5701 aagactagtt ttgatgatgt atttaaagta attaaagatc atcctgattt agataaaaaa
     5761 gtatttaaag ctacgaagga agttattcaa aatatcgcta caaacagcaa acttcgttat
     5821 aacacggcaa atgctagaac aaaagatggg aagcgaccag gtgcaaaccg ctttgatgaa
     5881 attcacgaaa atgaagatta ttcaatgata aatgtggcta cttctggtgg tggtaaaatt
     5941 cgagattata gagaatttta tgatacaact aatggtcatg ttcgtggtgg tccgcttgat
     6001 gacattatag aagaatcaaa aatgattctt tctggagaac ttggaattga caaggatgga
     6061 gcagaatttt ctagtttgtt tccatttatt tgtcgcttgg ataacgataa tgaagttgat
     6121 gatcccgaca tgtgggaaaa agcttgtcca actattaatt acaatgcaga tttaaaacgg
     6181 aaaatgtttc aagaatactc tcaaatgcaa cgtaatgctg gtttaagact tacgttcatg
     6241 accaaacgaa tgaacagacc tatggaagat acacgatttg ctgttgcttc atatgatgat
     6301 gttctgcata cgaaagaaaa agaatttcct gaaaaaatgg atgaagtgat aggaacagtc
     6361 gattttgctg atagacgaga ttttgccagc gttgggttgc taggaaaata cgataaagat
     6421 gtttatttta cacaacatac ttttatccac gaatcagccc ttcgattaca aaacatcaaa
     6481 cgagaggtta tagatatttc tatagatcaa ggaaaatcac agatcgttca tggaaaaaat
     6541 atagaagctg attatattgt aggttggttt cttgaaatga gtaataaata ttatattaaa
     6601 aaaatcgcta tggatatgta ccgtgcaaaa atattgaagc ccgctttaga agaagcaggt
     6661 tttactgtgg aaattgttcg aagcggatct gttacacatg gtatgttaaa agatctggtt
     6721 gatgaccttt ttattaatca acgtttattt tttggtgacg atgcgattat gcgttggtat
     6781 tgcatgaatg tatatgaaga gcatatttct aatggaaata tacgctatga aaaaatagaa
     6841 cctgaaacta gaaaaacgga tggctttttt tcattccttc atggtttgaa ttttttagat
     6901 gatatttatg attctgctcc tgtaacagtc acaaatagct cagtagaaaa tacaggaact
     6961 ggatttactc ctctagtatt ctaacttgaa aggaggtgag aaagtgggga tttttcaaaa
     7021 ggcggtagga tacttcacaa aaaaagcaac ggttccttta gaagaatact tttgtaaatt
     7081 gcaagttgat tttgtgtatc gaaaatttgc gattgaaact tgtattgatt tgattgcaaa
     7141 tgcgatgagt aaagcggaat tcaagtcata tgaagatgga aaaaataaaa agaatgatct
     7201 ttactatagg ctgaatgtag ctcctaataa gaaaaataat gcaacagaat ttagaaaaaa
     7261 actgatcagg agattaatat tctacaatga agtattgatc gtttctccgt ctaataattc
     7321 tagcgaaata tttattgcgg atagttggga tgtcacagaa tatgcattga aagatgatgt
     7381 gttttctcaa gtgcaaatta acaacatagt ccttgataga gaatttctag aaagtgatgt
     7441 tatctatata aaatacgcag atcaacaaat taggcaacta gtcgatgcgt attatcaagc
     7501 gtatgggaaa ctcatttcta gtgccatgaa tgtttacaag cgctctaacg ctcgtagata
     7561 cgtactgaaa gggaatttat tccgaccgca agacaataca acacaagatc aaatcaataa
     7621 aatgatgaca tcacaattta aggcttttat ggaagctgat aatgcaggtg cggtatttca
     7681 attacaaaat gagtacacat tagaagattt cagcggaaac ttccaaagca attcaagaga
     7741 tataaaaaac ttaatagacg acatctttga gatgacagca gcagcgtttc acgttccgaa
     7801 aaacctacta aagggagaca tgagtgggtt atcggatcaa gtggacgctt ttttaatgtt
     7861 cgaaatcata ccgattgctg aacttattca ggatgcgttt aacgctagtc tctatgaagt
     7921 agaagaatac ttgtcaggga attttgtacg tgtggataca actatgatca agattactag
     7981 cttcaaagat ttggttgacg ctattgatgt aggcattaga aatggagtat ttacaatcaa
     8041 cgaaggaaga gagcgcgttg gaaatgatcg ctctgataag gcgatggcag atgaaatatt
     8101 tataactaaa aacaatcaac aagtatcgaa aggaggtgag gcgaatgacg acaatgaaaa
     8161 catttctagc agtaaagaat gaaggcgcag taccgcaaat ttttattcag ggatttattg
     8221 gttctagttg gttctttgaa gggaatactg acaagggaat caaaaatatt ttggatagtc
     8281 taggtgatca agaagaaatt gaagtagtaa ttaattcaaa cggtggagac gtatttcaag
     8341 ggattgctat tgggaactta cttaagtcaa ataaagcaaa agttaacgtt gtgattaacg
     8401 gcttagccgc tagtgctgct tcaattatcg caatggctgg cgatactata aaaatctaca
     8461 acaatgcaca attgatgatt caccgcgctt ccacatacgg agaaggaaat gtcgatgact
     8521 tccgtacgat tgctgaccaa ctggaatcaa ttgataaatc ggtaaaggct tcatataaaa
     8581 cacgattcaa tggcacagat gaagcattgc aagaacttct tgaaaaagaa tcgtttatgg
     8641 atgcagaaac agctttgagt tatggattgg tcgatgaaat tatcgatgca gaaaatagct
     8701 caggtactga agctaaaaaa gaacaaagcg ttgaagaaat tttgaatgac gttaaagaaa
     8761 aaagagcaga aaaaattgct gcatttacag cagcattaaa taaaacattt ggacaaggag
     8821 atgtaaaata atgacagtta aaaatttaaa aggtgtaaca gctgcaagcg accaattgat
     8881 gaaagctttt aaagatggta acgaagaatc ttttagcgca gctatggtaa gcttatctaa
     8941 ggaaattcag gataaaattt tagaagaagc aacagcaaaa aatcaagatc aattagtatt
     9001 aatgaaccgt ggtcagcgtg tattaactac acaagaaaca aaattctata acgaagtagt
     9061 gaaaaacgaa ggttttgcag gggtcgaaga attagtgcca gctactgtat ttgaacgtgt
     9121 atttgaagat ttagaacaat ctcatccact attgcaaaaa attacttttg ttaacacaac
     9181 tggtgtaaca gaatggattg tgtcacgtgg agtcaatcca gcatggtggg gtaaactttg
     9241 cgaagctgtt aaaaaagttt tagataatgg ctttgatgta attaacatga agcagttcaa
     9301 gctatcaggt tatattcctg tatgtaaggc aatgcttgat ttaggtccag tatggttaga
     9361 tcgttatgtc cgtactgttt tagtagaatc attgagaatt gcattagaac aagcaattgt
     9421 tgatggtact ggtaaagata tgccagtcgg aatgatgcgt gacatgagca aacaaactag
     9481 cggagaatat gctgaaaaag aagcagaacc tattacagct ttagatgctg taactatggg
     9541 cggtttgatg gcgcgactat caaaattcaa tatcgaaggt gtgaatgatc cgatttatcg
     9601 taatgtgaat ccttctgatg tggtcctaat tgtgaatcca actgattact ggtctaaagt
     9661 attcccagct aagactgtac taactgctaa tggagaatac gtacaagtat tgccagtacc
     9721 agtttcagat ttgcagtcaa cggctgtgcc agaaggaaaa gcagttattg gggtagcctc
     9781 agattacttc atgggtgtag gatctacact aaaaattgaa gcttcagatg aataccattt
     9841 tgttgaagac gaacgcattt atctagctaa acaatatgca aacggtcaac ctaaacgtaa
     9901 cgatagtttc attgtattag atattagcgc tttgggaact actactacaa ctacaaaacc
     9961 aacaaccaca acaactacaa cacaagcgta ggtgatcaga atgaagtata ttctttgtca
    10021 gccggcaatc aatcggttta aatgggagct tgaagtttgt ttaactaatc tgaagaaact
    10081 aggaatcaaa gatatcgtat tgcttttcag cagacacgat gatcagattc ctattttttt
    10141 tgagaaggaa tatggtgttg aagttcatgt gtacgatgat ctgcgggacg acaaagagta
    10201 tattccttcg attaaaccat atttatggtg gaaatattta gaagaagatc attcgcgtga
    10261 ggacgaccga tatttctata tcgattcgga tgtcattttc aataaaagaa ttaatttgcg
    10321 caaattgcct tctaaagatg atgtttggta ttgtagcgac tgctgtagtt atctaagtct
    10381 tgattatatt agaagctgtg aaaacggaga aaatattcta aaagatatgg caaacattgt
    10441 aaatgttaca gtagaatctt tggaaactat aaacactaat tcaggaggcg cacagtgggt
    10501 tattaaccgc cctaaagcga attattggaa aaaggtttat ctggattcta atcggctata
    10561 tcgctacctt agagggcaaa aaacaaatat aaaaatctgg acagccgaga tgtgggcaca
    10621 gctttggaac atgatgtatt tcaatattgg tcctaaagtt cacgaggaat tagacttttg
    10681 ttttgctact gatccaatag aaaaagttaa agaagtaaaa atcttacaca atgctggagt
    10741 aacaacaaac gatgaagatt tatttttcaa agggagatac gtgacttcta cgccttttga
    10801 tgaagattta tcatttgtaa acaagaaaaa atgctcttac gcatatgtta aagcaattaa
    10861 ggcggtggtt agatgacgcc tgaacaagtg actgaagaat tgctaatagc tgtgaaggat
    10921 aatatttacg ttacctggaa cgaagaagat gagtcaatta aaaagatgat agctaaaaat
    10981 gctgtttatc ttcaaagtaa agtgagtaca acactttctt tttctcctga aagcttagaa
    11041 tacggattgc taatcgaaag atgtagatac gactggaatc gtgctttaga tgagtttgaa
    11101 caaaatttcg ctagtgagtt attaggtttc attcaacatt atgcgctaca agaatatatt
    11161 gcaggtgatg tgaatggcga ataatcgtag actcgaagaa acattcaacg atggttggtt
    11221 aaagattttg acgcaaacta ccaaaagaaa tgaactagga aaaaagattg gtgtagaaga
    11281 tacagaaatc acttctttaa aatttagaaa tctttccatg agagatagtg atataacagc
    11341 tatggatgcg atgggatcga aattaactaa gaaagtaaag actccatttc atccaatcgc
    11401 caagaaattt aataaagatc aatattttat cgtaatcgat agtatgcgtt acaacgttat
    11461 ctatgccgat tacgataatt tttatatcta tttttatctt gaaagtgtgg gtgaatatgg
    11521 tgattgataa ttctaaagaa aaagaacgtt taaataagca aatttctgct atcaaaactt
    11581 ccttagaaga gcattttggc ctcaaactct ttcaagactc cgtaggcgag gatgagctac
    11641 ctgatgattt taattacttc attctcgaaa caggagaaat agaaatgatc actgagccaa
    11701 aatatagcgt gggtcaaaat ctatatctaa ctttctattc agaaaataga gaagatttaa
    11761 caggagattc actagatatt atttcattga ttcaaaatcg ttcgattcgt tttcagagaa
    11821 tggatcccaa tcatttaaaa ctagagaacc aagatcgcta tatcgatcaa ttggtattta
    11881 cgtttagacg attattgaag agtgattgtc atggctaaaa atagttggga gctaaaaata
    11941 aatggacatg atgaacttct tgtgcggatg gaacgctatt caagcgagag cgaacgactg
    12001 attaacgaag cattgaaatc aaaaggttcg gctattgcag tggataggat tacagaaaaa
    12061 attcctgttt ctgaagcaga tttaagaaga gggcaccaac acgcaaaaaa tagtcgtcca
    12121 cttaagactc aatacattaa tttgggtttc atcattagac ctacaagaaa atttgagtat
    12181 ttaaaatatc ctgatttggg gataggtact tctaaaagaa atcagccgga cgaatttatg
    12241 agaagaggat taggtcttgc acttgatcca attacagaac ttctgattcg tcaattcgat
    12301 aaattaaata aataggagga acaacaatgg ctaaaacaac aacagtaaca acttttgaca
    12361 atataagtat taaaagaatt tcttttaatt ttaagaatgc aacaaatgca atttcaactg
    12421 attgtaatgg acaattagat ggcgaaacag aaatgcagag aattgtaaaa aaatgcggtt
    12481 caacagaagt aaaatcgaaa tctaaaccaa tcaatatgac ggtaacaatt actgcacatg
    12541 taccgatgga agtttatcga cgcttcaatg ggttgaaaca agatgaacgt attaaatcgg
    12601 gaatttactc ttatggtcct gattccgtag gggaagattt ctctcttgct gcagagatcg
    12661 tggatgattt cgaagaaaaa aataagctga ttggtatgtt agcatgcact tcgaatacag
    12721 gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa
    12781 aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca gaacttgaag
    12841 aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa aaaacttcag
    12901 ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa
    12961 gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc taaactgaat
    13021 atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc aaaaatgact
    13081 gtgggaaaag gatccatgga tatttctgtt attcaaggtg caaaggctgt gtatattgct
    13141 taccgccaag cgaacatgac tgattatatt tcattcgatg aatttatcga taaatgggat
    13201 tttgatatgg ctactgccag ctatatttat caattgatga tgttcaaaca agcacgcgat
    13261 gcttatcaaa aagaatttga aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc
    13321 caaagctctt agttgaaacg tgggtcgatg tctattcgat gttgaccgac gttttttcta
    13381 tgccttcaga tttggtttta agcgatatct gtttagatga cattttgcaa atggcttaca
    13441 acaagagtgc ttatgaagga tggaaaaact atgcaataaa ccaatcccag aaaaactaaa
    13501 gaaaggaggt aaaaaatggc taaaaagaga acagaagcag aagtaacttt catagctaac
    13561 gatgacggat tgaaatctac gttaaaagaa atcagtgctg aattaactaa aaatagagca
    13621 gaattaaaac tagaacaagc tcaattacaa cagactggtt ctgaatcaga caagttagga
    13681 agtaaattat cttctttaga aaagcagtat gaattacaaa gtcaaaaagt tgaagtaact
    13741 agccaacgtt tagccaatgc gaaaaaatat tatggagaaa attccaccga agttcagaaa
    13801 cttgaaagag aactgattaa tcaacaaaca gcgcaacaac gtttgtcaaa cgaaattgat
    13861 aaaacgagta atgcactagc tcaagcaaaa ggcgaaatac agacgtacga gtctacaatg
    13921 caacagttgg atagcgaaca aaaaaatgtt caagctagtg cttctctgat tgaatccgaa
    13981 tacaaaaaat ggcaagcaac tgctggtcaa tcagcttctg aagccgagaa attagcgaaa
    14041 gcccaagaat atgtttctca acaatctgaa aatgcggaga aaacgataga tatcctaaga
    14101 cgacagttag aagctacaca gtctgaattt ggcgctacat ccacagaagc aatgcagatg
    14161 gaagcgaagc ttaatgatgc tgaacgtgaa tttgaagagt taggacaagc tgctaaaaat
    14221 gtagatacaa ctaacttgga cgatatcgga agcaaaatag atatgaataa tttaatggaa
    14281 gcttctgacg ttttaagcga cattggcgat aagcttacag aattagggaa acaagcagtg
    14341 gactctgcta acagtgtagg tagttcccag agtaagatac aagctaattt tggtttgtct
    14401 aaacaagagg ctgaagaatt aacgaatgta gccagagaca tttattataa aggttttgga
    14461 gaatcgttag atcagtccac agatgcattg attttggtaa agcgtaattt aggagattta
    14521 aataatcaag atttacaaaa tatcacggaa caagctatgg tcctagaaaa caccatgggc
    14581 gctgatatgg atgaaacgtt acgtggtgta aatggcttaa tggtcaattt cggcttgagt
    14641 gctcaagatg caatggattt aatggtttcg ggtactcaaa acggtttaga taaaacgcac
    14701 gaattaggcg acaatatggc agaatatagc caattatgga gtcaaatggg atattcagct
    14761 gatgaaacgt tcggaatgct tcaaaatggt ttagatgcgg gtgcttataa ccttgataaa
    14821 gtcaatgact tagttaagga aatgggaata tcgttaacag atggtcgatt tgagcaaaac
    14881 atggatatgt ttagtgaaag tactagaaaa gcttttgaag agtggaaaaa tggcggagga
    14941 acacaaaaag acgttattaa ttccatgatt caagatttta gcaatatgga tggtcaatac
    15001 gaccaattaa ataaagcttc tacaatttgg tctgcacttg gcgaagataa cgcgatgaaa
    15061 gttgtccaat ctttgactga tgttaatcat acatttgatg atgttagtgg atctgcacaa
    15121 aaaatgaatg aagattctac taccccgttg caagagttga acggtaaaat agctgaatta
    15181 aaggattcat tagctcctat aggcaacaca atcatagatg cactcgaacc agtaattgat
    15241 tttctaggaa agatggctga tgcgtttaat aatcttccac aaccagtaca ggattatgcc
    15301 gtagcgattg gcggattgac tgctgcattt actttattaa tgccaataat agttggcttc
    15361 atggctctag gtggtcctac tacattaata ataggagcag ttattactgc tattgctgga
    15421 gttatagcaa ttataaaaaa ctggggcgca attactgact ggtttaaggg aatatggagt
    15481 aaattcactg attggttggg tggtacttgg gaaagtataa aagaaggtgc ctcatcagtt
    15541 tgggatggag tcaaagaaac ctggtctgga tttgtagatt ggattcaaga tatttggcaa
    15601 ggcgtttctg attggtttgg agagctgtgg agcggattag ttgaaggagc ttccaacatc
    15661 tggcaaggag tccaagagac ttggcaaaca ttcgttgatt gggtttcaaa tatttggaac
    15721 ggagtcaaag aagtatggtc gattatttgg gcagatattg tagggattgt tcaaatacca
    15781 tggaccttaa taacgtcatt gattcaagct ggtattaata ttatagtggg tatttttgat
    15841 gtagctggac agttattagg cgcagcttgg caagctgttt ggacacctat ttctgatttc
    15901 cttaaaaaca cttgggatac tatgacacaa tgggtaagca tcgcttggaa tggaattgta
    15961 actacattcc atactatatt tgatccagtt gtggcatggt ggaacggtat atggacatcc
    16021 attagtacta cggcttcaaa tatttggaat tcaattagtg caacagcttc tagtatttgg
    16081 aacagtatca agaatacaat cactagtttg gtacaagcag ctgctacagt aattcaaaat
    16141 atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag ctctacagca
    16201 tcaaatatct ggaatagtgt gactagtagt ataagcaatg ctataaacgc agctaaaagt
    16261 gccattcaaa gtgtttggaa tagtatatct tcgtggatca gcggaatttg gaacggtatc
    16321 aaaaacactg ctttgaatct ttggaatgga attacaagca ctattagctc taaagtaaac
    16381 gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt ttccgacata
    16441 ttcaataatg ttaaaagtac aattgctaac atttgggaag gtatcaaaaa gactgttagc
    16501 gctccgattg attggattag agataaaatc agtggtattt ttgataattt gaatatttct
    16561 ataccacata ttccgttacc acattttaaa ttgagtgggg aattcaatcc attgaaggga
    16621 aaaatcccaa cgttgggtgt tgattggtat gcgaaaggta gtgtgtttaa ttctccgaat
    16681 attatcggtg taggtgaagc aggacctgaa gcagttttac cattgaaaag atctgtgctg
    16741 caagaaattg gtgatcgtat cttgagtagc acatcagttt catctagggc acaaacgatt
    16801 caacctgtga acaattacga attcaatttc acaattgatg gtaacgcaga tgaggttact
    16861 atgaagcaaa caactcaaca aatcattgac agcattacaa aagttcaaaa tgataatgct
    16921 tcggcatggc gttaaacagg agagtatttc tcctgttttt tagtattaaa aaggatgtga
    16981 aaaaatgact gattgtatac attctataat cgatggattt cctgattatt tgcataaatt
    17041 ggctttagct gaaagaccaa ccataccatc tccaaaaaga cagagagttg aaacttctgt
    17101 tttaggacgg ttaggtggct tagtacaaga ttattcgttt gaagatatgt cgtttacttt
    17161 gcactataac tatttagagg atgcggaaga ccatcaagcg ttcaagcaat cgttttatat
    17221 catgcgtcat tggttaaact atgcaaagaa attagaattc tctgatgatc ccaacgtcta
    17281 ttatgttatc cagactatcg atattgggga tgcagaaaac gatattgttg aatggggaga
    17341 gttcgatgta aatattactg cgaaaccatt cgcaagagtt caagaagatg tgcctataac
    17401 cgtagataaa ccacagtcat ttagtttgct gaataatagt ttagaagaaa gttttccaaa
    17461 gattatcatc actccttcag ctacttcatg ccagttcatc ttaaatgatt atgtgtttag
    17521 ttttgaaggc ttagtagtag gaactgatgt agtcattgat agtgatttaa tgctttgcta
    17581 cgaagagcaa tcggacggag atattttaga tcgatccaac aaaatgaaga ccatgcaata
    17641 tccgacattg caagtggata ttaattattt taattgtact ggtttgagca aaatacaaat
    17701 ttatcgtaat gggttaaggt aggtgaaata gatgatcgat aatttaataa ctatttacga
    17761 taaaaatgac gcgaataatt tagctgaaca tttatatgat acacaaggtt taggcgcttt
    17821 gtcagactgg ttaacagcca ctgttagcaa taaattaaac ggagccgaga tatttcaggg
    17881 tacttatcca ataagcggaa ctaatgcaga cttgattgta gacggacgta ttattcagtg
    17941 ctatgtggat gaaaatcgag caaagcaacg tttacggatt tattatgcaa agacttctgt
    18001 aataggaaat atgatagaag taaaagctga acctattttc aatgatataa gaaaatcggt
    18061 gttgaataaa tatgacagcg gaacagaaaa gatcactgct actcaggcat ggcaaaacgc
    18121 aaaagtttta gcaaaaccag ctattccttc gcagttttct ttctcatcgt tagtagatac
    18181 gcttgctaat gtgaagatag aaaaggcgaa ttttttagaa ttctttggcg gaaaggaagg
    18241 atctatccta gatcgatttc atggggaatt tcttaaagat aataacacat tacgtcatga
    18301 aacaaggcta ggtacggatc ataaaatcaa agcgatttat actaaaaact taactggtct
    18361 cgacttagag atagatgctc aaagtgtttt agttggagtt tatccattca ttagcagttc
    18421 gtcagaagga gaggatgaga tcactctacc agaagaagtc attttcacgg attacgtgga
    18481 agattatcct gctggatatg tttcttttgt tgattttaaa gacaaagcga ctgatgtggc
    18541 cacattaagg gaagctgcta aagactggtt gaaaacaaac atagataaac agaaaccaca
    18601 agtgagtggt tcgattgaat tagtaccatt gaggcatcaa agaggctatg aaaaatttgt
    18661 tgatctagaa aaagtttcga tgggtgacgg agtagatgtg tatcatccac agttaaaagt
    18721 gaatatgtca gcaagaattg tggaatatac gtttaatgtt ttaaccaatt catacgataa
    18781 attagttgta ggaaacgtca aaacaaactt cttagaaaat acagagaata atgtcaacaa
    18841 tttgattaat gatgccattg atcaattgaa aaatggtggc gaaatcagtg atttaatcaa
    18901 tgatattgta gatcatcaaa ctgacatgat tactggtcaa gatggtgggt atgttttact
    18961 agatcctaaa gaagcaccta gtcgtatttt gattatggac acgccagata agaataccgc
    19021 aaggaatgtt ttacaaatca acaatgctgg tattggtttc tctaaaactg gtattaatgg
    19081 aacgtatgac accgcatgga cgttagatgg cggatttaat gcctcgttta ttacagctgg
    19141 tgagatagta gggattacta ttagaggtac tacattaatt agtgatggta ctgattatag
    19201 aacaagtatt gccaatggca aaatgacttg gtactcaaaa aaagttaaca aagatattat
    19261 ggagctagaa gcacgtgatt atgtaagtgc tgatgccggt attgtatcat acaccatgaa
    19321 aactggtggt ggtttcatga ttagaaatcc acaggggaat ttggttttta gtacgtggga
    19381 taatggcaat aacaaaccgt ttctatcttt tggtgcgccc aatttcaggt atagcaatgc
    19441 tagttatata actgatggcg acggtagttc tttaagcatt aatggtagtg cgggtagttc
    19501 atgggagttt aaagtagctg gcaggactat gaaatttact agtgatggta tgctaacgtt
    19561 gccaggttgt ttttttggtt catgggaaga tgggaaaatt gcaaggtttg aacaatcaac
    19621 ggtacaagta tataaaaatt ttactgttag aggtactaaa aactcaaccg taccgacaga
    19681 acattatgga caacgactat tgaacgctta tgaaactcca gaatattatt tcgctgatta
    19741 tggagaagcc gttacaggtg atgatggtaa agttcgtgtt gatattgatc ccatgtttgc
    19801 tgagacagta aatctaagtc ggtatatgac acatgtgaca cctacagaac tagttttgtg
    19861 tgctgttact catgaagatg ttgaccattt catcattgaa actagtaagc caaacgtatt
    19921 agttagatgg aatttagtgg cacaccgtct agggtatgaa gatattagat taaaagagga
    19981 tacagcatat gatagcacag tgcttgacca aaaacgtttt taaaacgaag acaaggaggt
    20041 atataaatgg ctagcagttt atataatttg gctttagatt tcagcaaaga attaaactac
    20101 accaaagcta ttatggctcg tcaaggtgat aaagggatta cggtgacggt taaaccgttt
    20161 ctaaatggct tgcagatgga tacgagtggc ggaacattta ctttaaaagg aacaacacca
    20221 tctaaccgtt acgtagataa tgttgcaact agtgtaacta gtgaagaagt cacgttttct
    20281 cttgatggca catttatgag tgaagcagga tattataaac actgctacgt agaatataga
    20341 aaagacaatc aaattttaac aacgcaagat atcatttttt tctcactagg agtgtctgac
    20401 atttcgcaag gccaagccga tgaatatgtt tcgcaattag aagagttgat tcgaaagtac
    20461 aacgaaactt ttgatgcttt tctggctgaa attaaaggta gaatggatag cttaaataaa
    20521 cagattactg atttaactgg tcaagctaaa acactacaag acaagttaga tgctctgaaa
    20581 gaagaaattt ctaaattagg taacttacat gtgatgtact ccaacagcat cgatttcggg
    20641 gaatatgatt attcggggaa tccgaacctt agcgctaaac ttaatgcttc aagtttttct
    20701 ttaggtactg gcgcaacagt cgcggatgat aatgatgaga ttgtgtttac cttagatggc
    20761 acaaatcaac tatcgaagta cgctagcaaa gcgcaagtgc cggtagcaga agggaaacaa
    20821 tatacgatta gttgtgaaat tatgttagaa gatgggttca ctggcgaccc ttcaggaata
    20881 cgtctgcaac acgcatattt gccaggcggg gttgttgttt tacagacaga gacagtacct
    20941 aaaaatgaat tgaatacgtg gcagacgctt atcggaacgc gaacggtaaa atacacctct
    21001 gacgtaccaa atgaatggta ccctttgttt agagacatcc gaacacttaa accgaccggt
    21061 aaagttagac taagaaaaat taagat
//
LOCUS       DACCXW010000044        20610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCXW010000044 DACCXW010000000
VERSION     DACCXW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.044"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            352..1041
                     /locus_tag="IVY71_001341"
     CDS             352..1041
                     /locus_tag="IVY71_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7985404.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IVY71_001342"
     CDS             1028..2236
                     /locus_tag="IVY71_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7985405.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IVY71_001343"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IVY71_001343"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7985406.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVY71_001344"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVY71_001344"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7985407.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="IVY71_001345"
     CDS             complement(6339..7034)
                     /locus_tag="IVY71_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7985408.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVY71_001346"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVY71_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7985409.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="IVY71_001347"
     CDS             complement(8233..8424)
                     /locus_tag="IVY71_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7985410.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="IVY71_001348"
     CDS             complement(8445..9335)
                     /locus_tag="IVY71_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7985411.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="IVY71_001349"
     CDS             complement(9322..10089)
                     /locus_tag="IVY71_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7985412.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVY71_001350"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVY71_001350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7985413.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVY71_001351"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVY71_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7985414.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVY71_001352"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVY71_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7985415.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14705..15043
                     /locus_tag="IVY71_001353"
     CDS             14705..15043
                     /locus_tag="IVY71_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985416.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="IVY71_001354"
     CDS             complement(15211..15969)
                     /locus_tag="IVY71_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7985417.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="IVY71_001355"
     CDS             complement(15987..16805)
                     /locus_tag="IVY71_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7985418.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVY71_001356"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVY71_001356"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7985419.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVY71_001357"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVY71_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7985420.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="IVY71_001358"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="IVY71_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7985421.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="IVY71_001359"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="IVY71_001359"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7985422.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5890 a         4211 c         3790 g         6719 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg
//
LOCUS       DACCXW010000045        20477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCXW010000045 DACCXW010000000
VERSION     DACCXW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.045"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(45..1184)
                     /locus_tag="IVY71_001360"
     CDS             complement(45..1184)
                     /locus_tag="IVY71_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7985423.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1583..2881)
                     /gene="eno"
                     /locus_tag="IVY71_001361"
     CDS             complement(1583..2881)
                     /gene="eno"
                     /locus_tag="IVY71_001361"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7985424.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2997..3752)
                     /locus_tag="IVY71_001362"
     CDS             complement(2997..3752)
                     /locus_tag="IVY71_001362"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7985425.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3856..5046)
                     /locus_tag="IVY71_001363"
     CDS             complement(3856..5046)
                     /locus_tag="IVY71_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7985426.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5156..6157)
                     /gene="gap"
                     /locus_tag="IVY71_001364"
     CDS             complement(5156..6157)
                     /gene="gap"
                     /locus_tag="IVY71_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7985427.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6198..7235)
                     /locus_tag="IVY71_001365"
     CDS             complement(6198..7235)
                     /locus_tag="IVY71_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7985428.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(7438..8772)
                     /gene="rpoN"
                     /locus_tag="IVY71_001366"
     CDS             complement(7438..8772)
                     /gene="rpoN"
                     /locus_tag="IVY71_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7985429.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8832..8903
                     /locus_tag="IVY71_001367"
     tRNA            8832..8903
                     /locus_tag="IVY71_001367"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8865..8867,aa:Arg,seq:ccg)
     gene            complement(9095..9979)
                     /locus_tag="IVY71_001368"
     CDS             complement(9095..9979)
                     /locus_tag="IVY71_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7985430.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(10066..10965)
                     /locus_tag="IVY71_001369"
     CDS             complement(10066..10965)
                     /locus_tag="IVY71_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7985431.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(10982..11488)
                     /locus_tag="IVY71_001370"
     CDS             complement(10982..11488)
                     /locus_tag="IVY71_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7985432.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(11677..12297)
                     /locus_tag="IVY71_001371"
     CDS             complement(11677..12297)
                     /locus_tag="IVY71_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7985433.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(12373..12810)
                     /locus_tag="IVY71_001372"
     CDS             complement(12373..12810)
                     /locus_tag="IVY71_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7985434.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            12973..13803
                     /locus_tag="IVY71_001373"
     CDS             12973..13803
                     /locus_tag="IVY71_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7985435.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            13787..15154
                     /locus_tag="IVY71_001374"
     CDS             13787..15154
                     /locus_tag="IVY71_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7985436.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            15173..15982
                     /gene="yidA"
                     /locus_tag="IVY71_001375"
     CDS             15173..15982
                     /gene="yidA"
                     /locus_tag="IVY71_001375"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7985437.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16025..16468)
                     /locus_tag="IVY71_001376"
     CDS             complement(16025..16468)
                     /locus_tag="IVY71_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7985438.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            16602..16973
                     /locus_tag="IVY71_001377"
     CDS             16602..16973
                     /locus_tag="IVY71_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7985439.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(17142..17924)
                     /locus_tag="IVY71_001378"
     CDS             complement(17142..17924)
                     /locus_tag="IVY71_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP7985440.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(17921..18892)
                     /locus_tag="IVY71_001379"
     CDS             complement(17921..18892)
                     /locus_tag="IVY71_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7985441.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            18984..20378
                     /locus_tag="IVY71_001380"
     CDS             18984..20378
                     /locus_tag="IVY71_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7985442.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT         5754 a         4225 c         3649 g         6849 t
ORIGIN      
        1 aataatgcta atctggctct gttttttcat ttattaaaaa aatcttacgc taagtaggat
       61 aaaataattc cttgatattt ttcgatcata tccaaataat tatcgatttc aacatactca
      121 tctacttgat gaggcaaagt aacgacacca ggcccaaaga cgacaaagtc gaaggaatgg
      181 ttagctttcg taaattcggc tgcatccgtt gttgctgccg ctccaactaa aggtaatggc
      241 tggctgaatt gttgctgaat gcaatgaatc aaaggagaat ctggatcagc tttgacaggt
      301 attttgttgt aatcgatcat caattccaaa tgatagtctg tctcttgatt caattcattg
      361 acgattgatt gcagcaatgc aatgatttta tcatttgggt attctggaat cgatcgaatg
      421 ttcccttgga gctgcgcatg gcttgggatg ctgttgactt gattcccgcc actgatcaat
      481 gtaacattat ggattgtttt tcctaggaca ggattctcga ttgtttcagc gaggtgattc
      541 atttcagcat ttgctttcgt aatgaattca tttaggtgat tgattgcatt ataaccttgg
      601 tctggcatag agctatgcgc ttcttttccg tgcgaggtga ctgtatagtt gattgatccc
      661 atgtgcgtat acatcaaact gtaattggtt ggctctccga tgatcaaggc atctaagtca
      721 tctacatagc ctgctttcgt gagctgttcc ccacctagtt cacccacttc ttctccgaca
      781 gtggctaata atttgactgt tccgttaaat ggttttcctg actctttcag ttcgatcatg
      841 gcaatcacca ttgctgccaa gccactcttc atatctgtag caccacgtcc gtacaaacgg
      901 ttcccatgga tttctgctgc aaagggtgca taggtccaag acgattcatc accagccgcc
      961 acgacatcca tatgcccaga taggcctaac acttttcctg attggccctt ttggtaacga
     1021 gcaatcaaat tatctctacc atctctgtaa gaaacaatct ctcctgttat gtcatgcttt
     1081 gccaaaagct tattcaaata ggctgctact tcaccttcgt ttccattcac tgatttaatt
     1141 cggatgatct cttgtaaaat cgcgattttt tcttcttttt tcatctaatc ctctcccttg
     1201 ccatttcttt ttattgtatt tgagtgtgct cgaattttca aataggagca accaaagtaa
     1261 aaatgaagaa agagagccac tttatgaaaa atatgctgct acttccctcc tattttattt
     1321 atacgagagg acttgctcat agattcctat tctactgttc tatccttaaa agctcttata
     1381 tgatgctagt tattgttagc tttccttttt caaggatgat cttttcttgt accattcttt
     1441 gtaaacatca tatacagaag ctagttgcaa aaaaactctc tacctgctga tatttcagca
     1501 agtagagagt tcgattactt aactaagtga tcatactcga ctatctttca atagtcgatc
     1561 atgtatcttc attagaaaag aattatttgt tttttaagtt gtagaaagat ttcaaacctt
     1621 tgtattctgc aacttcacca agttggtctt cgatacgtag taattggttg tatttagcaa
     1681 tacggtcagt acgtgataga gaaccagttt tgatttggcc agcgtttgtt gcaacagcga
     1741 tatcagagat tgttgaatct tctgtttcac cagaacggtg agatacaact gctgtgtagc
     1801 cagcttcttt agccatttcg atagcttcga aagtttctgt taatgtaccg atttggttca
     1861 ctttgataag gattgagtta gcaattcctt tttcgatacc ttcagataat ttttgagtgt
     1921 ttgttacgaa caagtcgtca ccaactaatt gaactttgtc gcctaatacg tcagttaatt
     1981 tcttgaatcc gtcccagtcg ttttcgtcta atccgtcttc gattgagatg attggatatt
     2041 tagaaactaa ttcttcgtag aatttgatca tttcgtcagt tgttttttcg ccttcgcctg
     2101 aatcagctag tacgtaaaca cctttttctt tgtcgtagaa ttctgaagaa gcagcatcca
     2161 tagcaagaac aacgtcttta ccaggtacat agccagcttt ttcgattgct tcgatgatta
     2221 cttcaaaacc ttcttcgttt gaaccaaggt ttggagcaaa tccaccttcg tcaccaacag
     2281 aagttgctaa tccgcgagct ttcaagattg aagctaatgc gtggaatact tcagcaccca
     2341 tacgtagagc ttctttgaat gttggagcgc caacaggcat gatcatgaat tcttggaagt
     2401 cgattgagtt atcagcatgt gatccgccgt tgatgatgtt catcattggt gttggcaata
     2461 cttttgtgtt gaatccgcct aggtagtggt ataaaggtac ttctaggtag tcagcagctg
     2521 cacgtgcaac agcgatagaa acacctaaaa tagcgttcgc acctaattta cctttgttag
     2581 gagttccgtc taaagcgatc atagctttgt cgatagccat ttggtcacga acatcgtagc
     2641 caatgattgc ttcagcaatg atgttgttta cgttgtcgac agctttagtt acacctttac
     2701 cgccataacg agctttgtct ccgtcacgta attcaactgc ttcgtattca ccagttgaag
     2761 cacctgaagg aaccattccg cggccaaaag ctccgctttc tgtgtatact tctacttcga
     2821 ttgttgggtt accacgtgag tctaagatct cgcgagcgta aacatcagta ataattgaca
     2881 tgttttgtct ctcctttgag tttgtttttt cctaagggat ttccctcata tacgatttta
     2941 gttaattttg ctcattagtg caaacatttc ttgcattttt cgcaaatttt caacaattat
     3001 tttacagcgt ctaacaatga caagaatgaa tctgcttgca agcttgcgcc accgactaaa
     3061 gcaccgtcaa cattttcttt tgccatgtat tcagcaatat tttctggttt tacagaaccg
     3121 ccgtattgga tacgaactgc ttctgatact tcttttccat aaagtttttc aactgtttga
     3181 cgtacaactc cacaaatttc gtcagcgatt tgtgcgtcag ctgatttacc agtaccgatt
     3241 gcccagattg gttcataagc aataaccatg tttgacacct gttcagctga aagatcagct
     3301 aaaccagctg taatttgtcc ttcgatccat tcagcagttt tgcctgcttc ataggtttct
     3361 aaagattcgc cacagcatag gattggtgtc ataccatttg caaagatagc tttcgctttt
     3421 ttattgatgt cttcgtctgt ttcatggaaa tattcacgac gttctgaatg tccgatgatc
     3481 acataatgaa cacctagatc agcaagagct gctggtgaag tttcaccagt gaacgcacct
     3541 gagttttccc agtagcagtt ttgtgctgag atttttagtt ctgtaccttc agctgatgtc
     3601 attaattctt gtaagaataa tgcaggagat ccgatgactg aatcaacttt gtcatttgaa
     3661 gggattttgt tttttactgc ttcagcaaat tcttttgctt ctgatgcagt tttgttcatt
     3721 ttccagttac ctgcgataat aggtttacgc attgaggaac acactcctta atttattgaa
     3781 aaatggctag ggagtcatga aaactccctt caccttttgt tcgattactc gtcagaattg
     3841 agcaaacttg gtcgtttatt tatcgttgat tgctgctagt cctggtaatt ctttaccttc
     3901 aagcaattcc aagcttgcgc cgccacctgt tgagatgtgt gtgaatttgt cagcaaatcc
     3961 taattgttct gctgctgcgg ctgagtctcc gccaccgatg attgttgttg cgccttctaa
     4021 gttagcgatt gcttcacaaa caccgattgt acctttagca aagttactca tttcaaatac
     4081 acccataggt ccgttccaaa caactgtttt tgcaccttgt aatgtatcag caaataattt
     4141 tactgatgct gggccgatgt ctaaagccat caaaccatca ggaatgtctc cttcgattac
     4201 ttgtgtttca acatcatttg aaaattctgg tgcacagaca ttgtcgattg gcaagacaag
     4261 tttgtcgcct gctttttcga ttaattcttt tgctagttcc actttatcag cttctactag
     4321 agagttaccg atcttgattc cttttgcttc gtagaatgtg taagtcattc cgccaccgat
     4381 caagatttta tctgctttag gaatcaagtt ttcgattacg ccgattttat cagaaacttt
     4441 tgcgccacca aggattgcaa tcattggtcg ttttggttct tctactgctt caccgatgaa
     4501 cttgatttct ttttccatca agaatccagc aactgttggg ataccagttg aagcgatacc
     4561 tacgttagaa gcatgtgcac ggtgagctgt accgaaagca tcgttaacaa atacatcgcc
     4621 taatgaagcc cagtatttac ctagttctgt atcatttttg ctttcttttt taccgtcaat
     4681 atcttcaaat cgtgtatttt cgaatacgac aacgtcgccg tcattcatgt tgcggattgc
     4741 ttcttctaat tgttctccgc gagtttcagg tacgaatgtt acttctttac ctaataattc
     4801 gcctagacgt tttgcaactg gagctaaaga ttttccttct ttatcttctt ctgtttttac
     4861 acgtccaagg tgagagaaaa ggattgcttt tccgccttgt tctagaacgt agttgattgt
     4921 tggcaatgct gctttgatac gagtgtcgtc agtgatcacg ccgtctttca aaggaacgtt
     4981 gaagtcaaca cggacaagaa cttttttgtc ttttaaatcg atatctttca ctgttttctt
     5041 agccatttta tttcctcctg aataaactta ttatcaaaaa aagcggaaga agcgcgattg
     5101 cttccccgct tttaaatgta catgtctata atttacactt gtacgtttgt ctatcttata
     5161 agttagcgaa gtattctaaa gtacgtacta attgtgcagt gtatgacatt tcgttgtcgt
     5221 accaagcaac agttttaact aattgtttgt cgccaactgt cattacttta gtttgtgtag
     5281 catcaaataa tgaaccgtaa gtcataccaa cgatatctga agaaacgatt tcgtctgtgt
     5341 tgtatccgta agattcgttt gaagcttctt tcattgctgc attgatttcg tcaacagtta
     5401 cttctttttc aagaactgtt actaattctg ttaatgaacc tgttggtaca ggaacacgtt
     5461 gagcagctcc gtctaattta ccgttcaatt caggaattac taaaccgata gctttagcag
     5521 cacctgttga gttaggaacg atgtttgcag cagcagcgcg tgcacggcgg aagtcacctt
     5581 taggatgagg tccgtctaga gtcatttggt cacctgtgta agcgtggata gttgtcatta
     5641 atccttcaac aacaccaaat ttgtcgttca atgttttagc cataggagct aaacagttag
     5701 tagtacatga agcacctgag ataactgttt ctttaccagt taatgtttcg tggtttgtgt
     5761 tataaacgat tgttggaaca tcgtttccgc caggagcaga gataacaacg cgtttagcac
     5821 cagctgttaa atgtttttca gctgctgatt tagaagtgaa gaaaccagta cattctagaa
     5881 cgatatctac gcctagttcg ccccatggta attcttctgg gttgcggtta gctaatactt
     5941 taacttcttt tccgtttacg ttgaatgaac cttcatgaac ttcaactgtt ccgttgaaac
     6001 gtccttgagt tgtgtcgtat tttaacaagt gtgccaacat tttagcatct gtcaagtcgt
     6061 tgattgctac tacttcgatt ccatctacat cttggatacg acggaatgct agacgtccaa
     6121 tacgtccaaa accattaata cctactttaa ctgtcattta agatttcctc cttatgaaaa
     6181 tcaaaaaaat atttatttta aagggttacc ccttttaaaa tctcatttgc agcagcttca
     6241 tcagtgatga gccacgtttg ttttggtgca tttttcatat aagctcgaat tgctttagct
     6301 tttgattttc cgcccgcgat tgccatgatg atcgggactt tttccaaatc ttgcaattct
     6361 atgccgattc tagggacctt ataaacgact tcaccttttt cattgaaaaa gtagccaaaa
     6421 gattccgcaa cagcatttgc ttgtttcagc atgactaatt ctttttcagt catcttacga
     6481 cgtgctgcca tgtgtaatgc acgcccaata ctatgaataa cacaattcgc ttcactaatc
     6541 aagtttaaga cttcttggat agatggctca ttgagtaaag aattgtaagt tgccagactt
     6601 aattgttccg gaacataaag tgcacggtgg ttaccgtttg ctttagcagc cattcttgca
     6661 ctgacagaat ttgcctgtac agttatcgct tcaccaatcc cgcctcttgc agggacaaac
     6721 aggttatgcc gtttcttatt ttccagatta gacaattgtt cggctaccac agccatagta
     6781 gttcctccca tcacagcgat gatattctcg ccttctggaa gtagtcggtc tagggcttcg
     6841 ctcagcgctt cgccgaatgc atcagccact ttggtctgct cttcgctgtt tcctgaaaca
     6901 acgatacagc gttggttacc aaaatattct gccaactgtt gttcggtttg atgcgtacct
     6961 aaaagctgat ccatgaagtt ttcaaggctt tgataaactt cttcgccctt agttgtcagc
     7021 gtcataccgc tttttgaggt gtcgatcaaa ttcagatttt tcaaaagatc tgtttctgtt
     7081 cgcaatactc gttctgtgat ccccatactt tccgataaac ttcgacgtcc aatcggctgc
     7141 atccaataga tatttcgtaa aattttatag cgttcttgca acacttcgat aatatctggt
     7201 gcaacagatt caatcatttt tatttcatcc agcatagcga tctccttagt tggacacttt
     7261 acgtccaatg tagacttaaa acgacccata tacgataaaa aaagaaatta actattcgaa
     7321 tttcacatca ataaagggaa atctcaacct cacgactgta agtctaacag ttttgctctc
     7381 gtttttcaac taaaaagact agaaaaagtg aaatactttt tctagtcttt tgactgatta
     7441 gttatcataa cgttttcttt ttgatgaact cgggattcct aataaatcac gatattttgc
     7501 tactgttcgt cttgagatcg ctatctcatc ttttttcagc aattcaacca atttctggtc
     7561 agacaacggc ttgtttttgt cttcttgatc gactaattcc tgcagctttt tcttcgctgt
     7621 atctgctgaa aggtcttctg tctgttctgt actattcgta ggaattcgtg tagtgaaaaa
     7681 tttttttaat tcaaatacac cgaagtctgt ttccaaatat ttcccgttga ctgcacgact
     7741 gaccgtggat tcatgcacat ctatctcagt tgcaacttcc tttaacgtaa gaggcttgat
     7801 tggacgttcc ttgtcgataa agaaagcttt ttgccgggaa acaatgactt gagcaacccg
     7861 caaaattgta tccccacgtt gtaggatcgt tttcttcaac cattcgaatt cctgtttccg
     7921 ttcttttaaa taattttcag tttctttatc tgcttgttgt gacatttgtt tgaagtaact
     7981 ctcttgaaag cgtatatttg gctgattttt tcggtttgag ataacttgta catttttatc
     8041 gtcgtccact aatacccgga catcaggaat aatgtacaat tcgctgctac ggtcgaaaat
     8101 ccggccaggg cttggactca acgtttgtaa atagtcgaat atctgctgaa cagcatgtag
     8161 atcaacagaa aaacgttgtg cgatttcttt ccattttcgc tccaccagtt cctcaaaaca
     8221 ttcttccaac acgatataag ctaattccgg cgcataatca tctcgttctg tttgaagcat
     8281 gagacattct tgcaaactac gtgcacctac tccagctggt tctaattgct gaatcagagt
     8341 caacgcatcg agcatttgga taggcgtagc tccaatctct tttccggctt cttctaaact
     8401 aatcgtcaaa aatccgttca aatcgatgta ttctacaaga taaagaacga ttttcctcaa
     8461 aaaagtatct cggtaattca aatggacttg ttcgatcaat gaatcaaata aagacccttt
     8521 tgtatcagga atctgattta ggtaatttgt ctcttctcct tcataagaag atgaagaagc
     8581 ttttatgtaa tctggctgcc attccggctc aacaacttcc accaatggat tttccattgc
     8641 ttgattctca acgaatgcca acagctcctc actgttatat tgtaaaactt ggatggactg
     8701 ttgcaactgt tgcgtcatgg ctagtttctg tgtctgctgc tgtttttgtg aaaaattttg
     8761 ctcaaatttc atttttttgt ccaatcccct tgtaattttt tatctgttta gttacaatta
     8821 tcccagtgct tgcgtcctta gtgtagtgga tatcacgtaa gattccggtt cttgagacgg
     8881 gggttcgatt ccctcaggac gcgttctact gctattttag cataatttac gaatagttgc
     8941 ttctttattt ttaagtgaaa actgtccgcc aaagaaaatt ggtggacagt tttttgttgt
     9001 acctgtatag atattgttaa gcctttgcaa gtaaggtagt tatgtgttca actacttatt
     9061 aaaatgtgtt atttattaaa gatacttgtc tcacttactg aactggccga tgatcgtttc
     9121 tgccataata gttctcgaca gccgttggat gccctaataa atccccaact gtcgtgatgc
     9181 gatacccttg ctctttcaga taatcgatca cttgaggaac ggcacgaatc gtttcaggat
     9241 agatatcatg gaataaaata attgccccat cgtatactgt tgcttggatc ttctgcaaaa
     9301 tcaaatcagg atttcttgtc ttccaatctt ctgaatcaac agaccattcg atgatcggtc
     9361 gctggatgat cgttgctcct acattcgtga cagaaccata gggcggacga acaaaagtgg
     9421 gggttttgcc agtgactttt ttgatctcag tgtctgcttt cgtcacttcc tccgtcatcg
     9481 actgggcaga aagagtggcc agattctgat gatcgtatgt gtgattgcca atctcgtgcc
     9541 ctgcttcagc tgtctgcttc acgatttcag gatgctgcgc cgtattttct cctaagacga
     9601 aaaaagtggc atgcacgttt ttttctttta agattcgcag aagctgcggt gttgtggtcg
     9661 ctcctggtcc atcatcaaag ctgattgcga cgcttttgac agccggatct cggctcgttt
     9721 ttgtactgtc ttgtgttgat tgtgatgact gactttgtac cttttccgct gattcttgaa
     9781 tggcttcttt gacgttagct gtttcagttg attggctttt aatggttgat tgttctgatt
     9841 gtttgaaaga ttgtgacgaa tgctccgaag agtgtgtttg tccttttact tcggtgatca
     9901 aggaggccac cgaagtaatt tcgaaaaaca aaagcacgga taaagcaata atactgataa
     9961 ctaaataatt tcttttcact atatctcttc tttctctttt tctcttctta ttatttactt
    10021 gtacttactt gaaaattgaa actgctagtc gcaactactt ctcgtctaat gatccaaata
    10081 atcttccgat gccaaaatcg tttttgtcac ggacttagga atgacaaatt ctggtgtacc
    10141 catataacca ggagcaattt cgtattgaga aaaagtcaaa actaattgat gctctttgtt
    10201 caaataaaaa cttcgtgtag gcgtaacaag tgttggtttt tctatcgtcc cttcttgaat
    10261 atcttgctca gtccaatagt acttagatgg atcttccttg acttgttcgg ccatctgccg
    10321 tttgacttct tttgtcaaaa cagatagata ggtgtcatct ttgaataaaa gtggaagtga
    10381 gagctggaca ctgtttttct tgtcgattgt gtcgtatctt ttttcaacat ggctatctgc
    10441 tgctgtttgg gtgatctgcc tttcaatcac gagaaaccgg ttatcgtcta cgattttctg
    10501 ataatctccc aaaacttggt aatgatccgt ttcaaaattt ccatattctt tttcagcttt
    10561 ttcaaaatct gcttgtcctt cgcgaaagta tttttgattt aatccatgaa ttgttttatt
    10621 ttctttctca ttttgtgtat tgatcttcgg tacctgaata tcgatttttt cagatcgatc
    10681 agtaaagcgt tcgcctaaga tcaattcaat gactgatcct aagatgggta aatcttctgc
    10741 aaagcttcga acttgactat taaaaagaag gctacttgtt gtaatcatta cgatagccaa
    10801 agatactccg aacccttgga gacgtctctt tctccgcttt gcttttataa atgattgctc
    10861 ttcgataata aaacgctggc ggatctcctc tttaagccct ttgggaattg cttgttcttt
    10921 ataagttttc tttagttttt ttaaattttc tttttgggaa tacatggcac tcttctctcc
    10981 ttcattctag gatttgacgt aaagcattca atgagcggta cattttcgtt ttaactgtgt
    11041 tttcattcat atccaagata gtcgcaattt ctttcaaacg aaatccttca aaaaagtaga
    11101 ggatcagaat ctctttttcc ggactgtcta ttttatctaa aatcatatgg agctctgcaa
    11161 cggaagtttc taaaggtaac tgttggggga tatcttgaat ttcaaataag tccacaggcg
    11221 tccttttttg tctgcgccaa ctatctaatg ccgtgcggat caatatccga taaaaccatg
    11281 cactaaaatg ctcgatctct ttttcttttt caaaatagtt cagggcttta tgaaaactat
    11341 cttgcatcac atctaaggcc tcttctttat catgtacgta attcatcgtg atacggaaca
    11401 ttttttgata atcagtttcg atgagtccat tcagcaggtg acggtatttt tcgatctgcc
    11461 gttttttctt atatccagta aaaatcattc tccactcctt tctgcctcac ttaatatgac
    11521 gtacaaggtg cgctaaaagt ttcaattttt ttatttttta tgtatttact tgggattttt
    11581 atatacaaaa aagaaggtgt gacaaaaatc gtgtcacacc tccttattct gataggctga
    11641 tcgtctctgt cacaacctct cgtaagaaaa taatgtttat tctttttcag agtcagaaaa
    11701 ttcttcctct tcataatctt catcatcatc gtcttcgtct atgatactta aatcttcttc
    11761 gatatttcct ggcaagtctt cttcatcatc tgtcgggtca tctgctccga tttcttgcaa
    11821 gtcagaatta taagcagcga tttcttcatc ttcatcatca tcgtcatcaa acagatcatc
    11881 gtcatcttct tctgtcagat ctgtatcttc tgggtcatcg tcattgtaat cgatcgcgtc
    11941 ttcatcatca ttgataaagg cgttgacttt cttgcgttta cggcgacgtg gagtatcttc
    12001 atcctcttcg tccattccat ggtttacttc ttcatcgatc gaatcgatag cataccatga
    12061 acgcaagccc caacgattat cccctaatga gataaagctg ccgtcaatat tcaagtcagt
    12121 ataaaattgt gcgagtgaat cacgaatttc actgtctgat ttcccaagat agttttggat
    12181 ctggttagct aaatcagaaa aatccatgac atcgccacgt tgttctaaga tcgcgtgtgc
    12241 tacttcgatc attgataatt cgcttttgtt tgcaccttca aatacactaa tttccaatca
    12301 ggacacgtcc tttcacagtc tactccttat catacaaaat cagacacaaa aaatcaaact
    12361 tttcttttat atttacgctg taaaatccaa ggtcaaataa tattcgccga ttttttccgg
    12421 tcccatgaac agatcataat cgattcgaac cttccctgct gtgggacggt ctttcaaact
    12481 aacatgcagt tctttcgtaa aagtagcaat ctggatcata ccgtatggtg ttcgatagga
    12541 tgtttctaac cgttctttat agccaaattt caaacgcatc cgcatttctc ctgcacggat
    12601 cagctgtaca tgaccatctg gaactaattt gatcgttaca ggaatctcat cgcccgtctc
    12661 ctcctgaatc tctttataac gtatgtacag cgtatcgcca attgtcacca tttgcccttt
    12721 caaatcaaaa taaaaatctt gcacttcccc ttgctgtttt acttttgtac gcaatttgat
    12781 tgaaatcggt gttccttgtg ataattccat tattcattct cctttacttc tcatagctac
    12841 attgttccta gtttcttttg ttttgtcaac caacttgtcc atttttacag aaaacctttt
    12901 ttccaaaaca actctttgat tttagagtat aatattgtag aaaatatgaa tttatacaaa
    12961 ggagccggtt gaatgaaccc tgcgattcga ctagatcagc acatttatca aaaaaacgac
    13021 ttttcccctt ttgcattaac agatatatta acggaatatt ctaaagaaca ccagcaaatc
    13081 tttttacatt tctggcaata tgactgcaca gtcatcttag gtatgaagga tgttcgaact
    13141 ccttttctaa gtgacgggat ccaaacactg aaagatgcag gttacgctcc agtgatcaga
    13201 aattcaggtg gattagggat cgtatcagat gaaggaattt tgaatatttc tttgattttc
    13261 ccccaaagtg acacaaaaaa aataaccata gatgacgctt acgagcaaat gctcctattg
    13321 actaaatacg cttttccaaa ggcaccgata caagcattcg aaatcaccca ttcttattgt
    13381 cctggaactt atgatttaag catccatggc aaaaaatttg ccggtatcgc tcagcgtcgg
    13441 atcaaaaatg ggatttctgt tatgatgtac ttaagtgtca acggtaatca ggaaaaacgc
    13501 gggcaagtca tgaagcgatt ttatcaatct gctttaaaag aagactttgg tacaaatggc
    13561 tatcctgcag tggatcctgc tacaatggca acacttgaag aactgcttga ccaacccctt
    13621 tttgtaaaag aagtcaaaga agcatttgca aaagcttttg aaaagctgta ttctgcaagt
    13681 gatttacagg attctaccga ctggctaaaa aaagaaatcc agctggagca atggaacaac
    13741 caaatccaga gaatggaaca acgtaataac ataaaggagc tacattatga caacccctta
    13801 taaatatcaa atgctgccta tggaaaaagt ttttcgtgat ccagtccata actatatcca
    13861 tgtccagcat caagtaatct tagatttgat caattcgaaa gaagtccaac gtctacgacg
    13921 cattaaacaa ttaggcactt cttctttcac ttttcacgga gcagaacata gccgttttac
    13981 tcattcatta ggcgtttatg agatttcaag acgaatctgc gatatctttt ctcgaaattt
    14041 ttcaaaagaa aaaataggca acggaggctg ggatgatcag gaacggctgg tgactttatg
    14101 tgcggcactg ttgcatgatg tcggtcatgg tccttactct catacatttg aacatatctt
    14161 ccatacgaat catgaagcca tcactgtaga gatcatcact tctccagaaa cagaagtgta
    14221 tcagattctt aaccgagtag aagaaggttt tcctgaaaag gtagctagtg tgatccaaaa
    14281 aacttacccc aacccacaag ttgtccaaat gatttctagt cagatcgatg cagatcgaat
    14341 ggactatttg cttcgggatg cttatttcac tggaacagaa tatggaacat ttgatcttac
    14401 ccgaatatta cgtgtgattc gtccttatga aggcgggatc gctttttcca tgagtggcat
    14461 gcatgcagtg gaagattata tcgttagccg atatcaaatg tatgtgcaag tgtacttcca
    14521 tcctgtttcc cgtggtatgg aagtaatttt ggagcatctg cttcatcgcg cacacgagtt
    14581 gtatcaagaa gatgccagca gttttgaact ccattctcaa ttacttctgc catttcttga
    14641 cggaagcttt actttagaag aatacctcaa acttgatgat ggggtcttgg gcacttactt
    14701 tactcaatgg tctgaatctg ctgatcctat tttaaatgat ttagctaaac gttttttgaa
    14761 tcgtaaacca ctgaaatctg ctactttttc tggcaaccgg gattcaaaat tagtccaaga
    14821 actcactttg ttagtagaaa aggtcggata taatcctgtt tattacactg ctgtgaattc
    14881 tagctacgat ctaccttatg atttttatcg cccagaacaa ggcagacatc gcacacaaat
    14941 cgagattttg cgaaatgacg gaactttgat agaattatca caggttagtc agcttgttgc
    15001 tgcattagcg ggacaagaac aaggagatga gcgatttttc tttccaaaag aaatggttga
    15061 tccgtccttg cgtgatcatt atgatttatt tgacgaaact tatcaagaat ttgccagcca
    15121 tatccgtaat ggcgcattga ttgaaataaa ttaaaatata ttggaggaaa ttatgtctat
    15181 caaactagtc gctatcgaca tcgatggtac tttattaaat gagaaaagag aagtaacaaa
    15241 agaagtaaaa gaagcgattg ccgctgctgt tgcaaaaggt gtttcgatcg ttttatgcac
    15301 tggccgtccc ttaccaggtg tccaggaaca gttaaacgag ttgaaccttt ttcaagataa
    15361 tgattacgtt attacttata acggggcact cgtacaacaa acgaaatcag gtaaaatcat
    15421 cgcccgtcac ggactgacac atgaagattt cctagagatc gaagtgatgg cacgccgtgt
    15481 cggcagtcat ctccattcga tcgatgacaa agcgatctac acgcctaatc gagatatcag
    15541 cgcttatacg atccacgaag caagtttagt caagatgccg ctaaaatatc gaacacctga
    15601 agaaatgaca ccagacatga acatcgtcaa aatgatgatg atcgacgaac cagaaatttt
    15661 agatgcagca attgcgcgtt taccgcaaac tttccgtgat aaatacacaa cggtcaaaag
    15721 tgcgccctat tacttcgaag tactaaataa agaagcaagt aaaggtgcgg cagttgcgaa
    15781 tctcgcacag caccttggta ttgatcaaga tgaaatcatg gctatcggag ataacgagaa
    15841 cgacttatcc atgattgaat atgctggtct aggagtagca atgggaaatg ccgtttctct
    15901 tgtcaaagaa gcagcaaatg tcatcacttc atcaaacgac gagcacggtg tagctgaagc
    15961 aatcaaaaaa tatgtactgt aataaataaa aaaactgaga gaaagctgat ctactcgctt
    16021 tctttcagtt tttatttttt tcgatccatc tctcgactgc ccatcggtgg ctaccacgtt
    16081 tcaatttcac aagcgcttct tgagggctca cccaccaaat tttattggtc ttttcagttg
    16141 gttctcctat acgcttccaa gaatcagcaa caaagaaata tcctggatta taataataag
    16201 ttgcacgata attcgagtaa aaatactcat cagcttctcc cagataagaa gaaatttctg
    16261 ccacaattcc catttcttct atcagctcac ggacaatcgc ttcttctttc gtctcttctc
    16321 cttcaatttc cccgccagga agaaaaaaag caccatttgg agcctcgatt actgcaatct
    16381 gctgatctct ttctatgaca atatatgctg cataacgggt ttgataatca gcaaattgct
    16441 ttttttcacc aaaaattggt ttatccatga atagtttcca ccttaacagt tttattttct
    16501 atccatttta catgagaaat ttctcatgaa caagcacaaa tcaactattt ccttacttct
    16561 ttttgttatg attgagtaaa aggaaaagga gagatgaatc tatgaaaatg gctcacacat
    16621 gtgttcgagt gaaagatctt gaagcatcaa tcgaatttta tcaaaaggca tttggttttg
    16681 aagaaagccg ccgccgtgat tttccagaaa acaaatttac acttgtttat ttgacacttc
    16741 ctggggatga ctatgaatta gagttgactt acaactatga tcatggtgcg tatgatctag
    16801 gagacggcta tggtcatatc gctatagcag ctgatgatct agaaaaatta cacgagcaac
    16861 ataaagctgc tggtcttgag attactgact taaaaggatt acctggcact gcgccttctt
    16921 actactttgt cattgaccct gatggttata aaatcgaagt catccgagga tagttagtat
    16981 ggggcatttt actcttgatt gaataaaaaa caaacaccaa taattaaaat gagacgcaaa
    17041 cttttcatta aattgaaaaa gtttgcgtct ttttttaaca accattgtag ctataaggtt
    17101 gccactctcc accaatttat ttttaattca tttgaaagaa atcaaataaa cgaggtacct
    17161 ttagaaaaca aaactgcttt tcctcctatt atcacacgat cttcttttaa ttcacattct
    17221 agatccccgc cccttgatga catttgataa gctgacaatt ttgttttatt cattctttct
    17281 gcccaataag gaataagatt agaatgtgct gatccacata ctggatcttc attgattgtt
    17341 aattttggaa agaaggttct agaaacaaag tctttttgca cactttctgc tgtcacgatg
    17401 actcctattc ctaattcaaa atttttgata gctgtaaaat ctggctgcaa tttagccacc
    17461 gttttttcat cttctaatac gaaaaataaa tcacgtgcta agtaggcttc tttaatctta
    17521 gccccaatcg cttcttcata ttcagacaat atcggcactt ttttcggcat aatactagga
    17581 aaatccatgt aataataatc tttaattctt gtcacaaata gattaccgct ttgagtagaa
    17641 aatttaatcg tttcatcagg tattttgtaa tagttaaata aaacaaatgc tgttgctaat
    17701 gtcgcgtgac cacaaagatc gatttctcga tctggcgtaa accatctaag ctcaaattct
    17761 tggtttttct tcactgtaaa tgctgtttca gataaattgt tttcaatggc aattttttgc
    17821 atcgtacctt ctggcagcca ttcttctagc acataaaccg ctgctggatt tcctttgaac
    17881 acctcatctg caaatgcatc aactacgtaa taatctaagt tcattttaaa gcccctttct
    17941 ctaatttgtt ataaatatat tctgcacaac aaagatcttc aatagctaga ccaactgcgt
    18001 cgaaaatcgt cggcctcgat tctcctttat aaaatgtttt tttctcagaa actaattcgc
    18061 ctaatgtacc aacaatacaa gattcctcta aatctccttc tgccatggct ataagcaggt
    18121 ccccgctttc tgttaaggca gctgagtaat catctacata gatttgagaa gtttccatca
    18181 acgatgtact tatctctctt gtatcaggag taaatgtgcc cacagcattg atatgtgcgt
    18241 cttcccttac ccactctttt tgaagaaatg cctctttact agaggtcaac gtacaaatga
    18301 tttccgctgg acgtactgca tcttctactg atattgcatt tataaacgtt attttaggat
    18361 actgtttttt agctttagcg ataaaagtat ttcgtctttc ttctaaaata tcgtaaacaa
    18421 acactgtagt gatgggacga atggtagaaa tcgcagtcaa atgcgacaat gcttgttgac
    18481 cagctccaat taaagcaagt gtttctgcat gcggatttgc taagtaatct gttgccagcg
    18541 cagatacagc agctgttcga atccaggtga ctgcatttgc gtttatcaat gctttgggta
    18601 gtccgttttg agcatcaaac aagaaaatct ctcctaaatg agaaggtaaa tgatactgat
    18661 gattatccgg aaaagcggta atgacttttg tgccaaaata tctgttctcc cccaaataag
    18721 caggcatcat cgcaaacata tttttgcctt ttccgtcagg taaaatttcc accaatcgag
    18781 gattttggct gattttacca ctttcataat caaaaaaaca ttttttcatt acttgaatcg
    18841 cttcttcaaa actaaaagaa gctactactt cctcaaaatt aataattttc atagacaatt
    18901 ccctcctcac aaaagattaa cttctaaaat caatacaatc aatggtaaaa ttgtatggta
    18961 tacaatttta aggagtggag aaaatgccta ttaattctta tgacaattac ccgttaactt
    19021 ggcgaccaga caaacaaaaa ttgaagcgtc cttattatcg atcgttgatt caacaattag
    19081 aacaagatat tttaaagaat aaattacaga aaaacactca gttaccctcc caaagagagc
    19141 tagctgatta tttagattta aattttacta ccgttggcaa ggcttataaa tatggaattg
    19201 agaaaggttt gctgtatacg aatattggaa gtggcacatt tatttctccc aatgtctttg
    19261 agtccatcac catctctacc aatgaagtaa ccgaacatat cattgatttt ggtctggtta
    19321 gttcttttga agaatgtaat aaattcgttg tcccgtttat tcaatcaact agtaataacc
    19381 caaatcttgt tggtttgctt aattaccaag atcccaaagg cacaaatttt caattgacta
    19441 ctgcagcaaa ttggttaaaa actcaaggag tcattccgtc cttagaatct ctatctattg
    19501 tttccggtgt tcaaaatgga ttggctatca cactttctgc acttttctct ccaggtgata
    19561 gaatagcaac agaccgatat acatacgcta atttcattga attggctcag ttatatcatt
    19621 tagagatcgt tccaattgat tttgacgagc aaggaatgcg acctgatata ttgattcagg
    19681 aatgtagaaa aaagaagatt catggtgtct ttcttatgcc ttcttgtaat aatcctatag
    19741 ggtttcaaat ctctcacaaa agacgagagc aattagccga agtgattgaa aaagaaggtc
    19801 tatgggttat tgaagatgat atccattcat ttttaacaac ttactatcaa catacgatca
    19861 ttcctccttt ccaaaattta ttacctaacc aaacaatcta cttagctgga atgacaaaat
    19921 ttatttgttc tggattgcga gttgcttatc ttgtagttcc agtacatgtc caaagcaaga
    19981 tagaacaagc aatttttaat ataaatgtga aaacttctgg attagatgtg gaagtcatca
    20041 ctcaaatcct ccattcaaat gaagcaaata ggatcttaga aactaaatta agtcttacaa
    20101 aaagagccaa tcagcttttt gattctcttt tcaaagtaga aaaaccaacg aatcgtgtcc
    20161 cttactatcg aattcttcca gttgatcctg agattgaccc taagataatt gagaatgact
    20221 ttcttgctaa cggtatccga ctttatcatt ccagacgctt tactacacgt acgcaaccag
    20281 atgcattcgt tcgaatttcc ctatcctcta ataaccttga aattttagct tctggactaa
    20341 gtatcgtaaa agaaaaaata aaaaaatatg aattataacc tattgataaa taaaggaatc
    20401 aaatcttatt gattcctttc aataaaaaag aggcactacc cgaggttcaa caacgggtag
    20461 tgcctcaaaa aataaac
//
LOCUS       DACCXW010000046        20399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCXW010000046 DACCXW010000000
VERSION     DACCXW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.046"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(387..1853)
                     /locus_tag="IVY71_001381"
     CDS             complement(387..1853)
                     /locus_tag="IVY71_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7985443.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IVY71_001382"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IVY71_001382"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7985444.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4370..4549)
                     /locus_tag="IVY71_001383"
                     /pseudo
     CDS             complement(4370..4549)
                     /locus_tag="IVY71_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(4753..5112)
                     /locus_tag="IVY71_001384"
     CDS             complement(4753..5112)
                     /locus_tag="IVY71_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7985445.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5142..5483)
                     /locus_tag="IVY71_001385"
     CDS             complement(5142..5483)
                     /locus_tag="IVY71_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985446.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5480..6154)
                     /locus_tag="IVY71_001386"
     CDS             complement(5480..6154)
                     /locus_tag="IVY71_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7985447.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="IVY71_001387"
     CDS             complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="IVY71_001387"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7985448.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8668..9858)
                     /locus_tag="IVY71_001388"
     CDS             complement(8668..9858)
                     /locus_tag="IVY71_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7985449.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9879..10406)
                     /locus_tag="IVY71_001389"
     CDS             complement(9879..10406)
                     /locus_tag="IVY71_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7985450.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10453..13242)
                     /locus_tag="IVY71_001390"
     CDS             complement(10453..13242)
                     /locus_tag="IVY71_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7985451.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13389..13589)
                     /locus_tag="IVY71_001391"
     CDS             complement(13389..13589)
                     /locus_tag="IVY71_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7985452.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14042..14362)
                     /locus_tag="IVY71_001392"
     CDS             complement(14042..14362)
                     /locus_tag="IVY71_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985453.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14865..15983)
                     /locus_tag="IVY71_001393"
     CDS             complement(14865..15983)
                     /locus_tag="IVY71_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985454.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16454..17761
                     /locus_tag="IVY71_001394"
     CDS             16454..17761
                     /locus_tag="IVY71_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7985455.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17882..19081
                     /locus_tag="IVY71_001395"
     CDS             17882..19081
                     /locus_tag="IVY71_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7985456.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19108..20376)
                     /locus_tag="IVY71_001396"
     CDS             complement(19108..20376)
                     /locus_tag="IVY71_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7985457.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5905 a         4344 c         3559 g         6591 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacatgact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaatttct tccatacaga tgttttggct tattctttct
     4381 ttttctcttc gatcattgcc cctgctctta tccaacccgc acttgccttc ttgatcttga
     4441 caggatagca actaagtttg aagcctgttg ctggcaattt gtctaaattc attaattttt
     4501 cgataggaca atatcttttt tcaatccctg caaaatgtcc ttcccagata ttcgaagctc
     4561 cactacgctt tcaaaaatat agaaagagtt ctttaatgaa aattatttca actttccata
     4621 aacagtaatc ccatataaat aaaagacgta aaagtcacat gggagaactt atacaggaaa
     4681 acacatagag tcaggtgaaa tacatgtatc ccacctgact ctatgcgttt tctaaccttt
     4741 attattctct ttttatacaa tttctgcacc gaatggcatc aacgccaatc gagcaagttt
     4801 gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga tgccgctgac
     4861 gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat taacgatcaa
     4921 tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag aaagggaggc
     4981 gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc aaagtatccc
     5041 cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga tgacatttcc
     5101 taaacaactc attgtatttc tccttctatt actgtttttg attaaaactg ggcaaaaatt
     5161 gattttattc ttcttttttt agcttccagc atcaagtatt ctgcaaattt tttttggatt
     5221 tcttccgtat tttcttttgt tactaatgtg tgttggatta gttttcctga tggtcgctga
     5281 tccacccgtt cttctgactg ttgcacgtcc gcttgcgcaa atccgataat ccgtttttct
     5341 tttgttacag caacgagcaa tagtttattc ttatcattta ggtcagctgc cgtttttgct
     5401 tttacgaatg attctggatg atcagtaatc agctgattga tttgaggtaa atcttctttt
     5461 actgcccacc gtatttccat catttctcac ttttctttcg ctcattttta tctccactaa
     5521 aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc aatgatctca tgtccgccaa
     5581 tcgttgggtg gaccccatct tctcttgtat atttcgaaaa accataagca atccctaaag
     5641 cattgaatat gccatctaaa ggaaccagca atgcgccata ttccttagca agtttacgaa
     5701 caatttggat tttagggtct aagtctttac gccagccatg acggtcgtcg gttataggaa
     5761 gtacaaacgg ctccatcaag atgatgttgc agatttccgc tgtttgaagt tcatttagta
     5821 attttcgata aacggattca aattgctgcg cagatttctc agttccgaat atagcctgat
     5881 cgctagtatt atgccaggta tcattgattc cgatcaaaat cgatacatag tcaggttgta
     5941 aagctaaaca atcttcttgc cagcgtactt gcagatctgc aattttgtct cctcctatcc
     6001 cacggttcat gcattcaaat ttcatcgttg gatattggga gattagtttt ccggcaatca
     6061 aaagtggata acctttacct agatcagttg cttctgctcg gtctcttcct gcgtctgtaa
     6121 tgctgtcacc gataaataat atacgttttt tcatttgtca aaaactcctt tttgaataaa
     6181 acgatgattt tcttccttat attatcccat aagccctatc aagacgaaag agaaaatata
     6241 tgtataaaaa atgacaccag cctagtccct tcaatcaatt gaatgaacgc tcatcaagtg
     6301 tctagttttt tatacttttt ttcttaacgt gccggattcc ccttgattcc ttcgttgttt
     6361 ggtccagaga aatcgatcac acctaagctt ccgtcaatca gaccactgat gacgccatca
     6421 gaacatatat ttataggctc atctaagact tccagtaaat gttcttttac taatatctct
     6481 tctagattgc ttgggataga ttggtcataa ctttatctgg tccgatgatt tcaaggatga
     6541 attaaactta catatggatc attgcatgct gaaaatacgg acctactcca tcttcgaatg
     6601 ttactattag tttgttttca ggtactagat gctgctttat ttcaacagca gaagcatcat
     6661 caaatcagat ttccatttga ataaccttct ttgtatatgt ttgattgatt gcgatttcgt
     6721 tatagaacct tgtgaatcaa atagcaattt cttttgcttt cttctcttgt ctgctggaaa
     6781 cttcctttat cccgtatttt aaaaaaagaa ctgcgagagt tcgcagttct tttttttaaa
     6841 taaacgttgc tctccggcca actcctcggt cacaagtcac aatagtcatc aaacatgaaa
     6901 aactgttttt tgaaattact ccttgtgact cggagctgaa cggcaggcgc acaacctctt
     6961 gatataaacg gtgttcaatc agctgaccct cgatattagc taaaaaaacg gtaaaatatg
     7021 agaagctatt tttccgtttt gtttctctaa tactcaggtc aaggcactga tttcacaacc
     7081 tcttccttaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     7141 tgtggaaagc catttccaca aaatttttct taatactcga aactaaacag ctttttcaca
     7201 acctctccac taaacggtat tcaaaagctt acatctgcgg taataagtcc cgccaattca
     7261 aaaacatgaa cagctgtttt tgcttggctg ttctaattac ttgatgcaga acagcttttt
     7321 catcacctct tatggataaa ttcggttcaa caaacgtggg aatggaatag cttcacgtac
     7381 atggtcgata ccagcaatcc aagtgactgt tcgttcaagg cctagaccaa aaccagagtg
     7441 cggaacagaa ccgtatttac gtagatcaag ataccatgaa tattcttttt catccaaacc
     7501 gtgattacgg atttgttcta gcaagaaatc atagtctgtt gcccgttcgc ttccgccaat
     7561 aatttcaccg taaccttctg gtgcgatcat atctgcacaa attaccacgt cttctcttgt
     7621 tggatgtggt ttcatgtaga atggcttgat ggcttttgga tagtttagga taaagacagg
     7681 ctgttcgaat gaattcgcaa tgaacgtttc atgaggtgaa ccaaagtctt cgccccattc
     7741 gatgtcatca aatccatttt tcttcagtaa ttcgattgca tcatcgtaag taatacgagg
     7801 gaatggcaat tttgtataag tttttaagac ttctttgtct cgtcctagta catctaatgc
     7861 atattcgcaa tgatctaaaa cattttgtac taagaatgct acatattgtt cctgtacttc
     7921 taaactttct tcttgatgag tgaaagccat ttctggttcg atcatccaga attcgatcaa
     7981 atgacgtcgt gtttttgatt tttcagcacg gaaagtcgga ccgaatgaaa agacttttcc
     8041 gaaagccata gcagcagctt ccatgtataa ttggccactt tgagataaat aagctttttg
     8101 atcaaagtaa tttgtttcga atagatccgt agtgccttct ggtgcagaac cagtcaagat
     8161 cggcggatcg attttgacaa aatgatgttg attaaagaat tcatacgttg cacgaatgat
     8221 ttcatttctg atctgcataa tcgcatgttg tcttgaagaa cgcagccata aatgacgatg
     8281 atccatcaag aaatctgttc cgtgttcttt tggtgtgatc ggataatcgt ggctctcgct
     8341 taccacttcc acatttgtaa ttccgatttc ataaccaaat tttgaacgac tgtcttcacg
     8401 gatttctcca gttactaaca cggatgtttc ttggtgtaga tttttagctg tttggaaaac
     8461 ttcttccgac acttcgcttt ttacaactac accttggaaa aaggctgttc cgtctcttaa
     8521 ttgtaaaaat gcgatttttc cgcttgatcg cttatttgcg acccaagcgc cgattgttac
     8581 tgtttctccc acatggtttt tcgaatcgat gatttgaatt tgttccacgt atgactctcc
     8641 ttttatattc cttgaatttc ttcaggatta attttgactt ttactctcaa taaattgctt
     8701 gattctgcgt accgcttctt ctaacgtagc gcgatcagtg gcataactca tgcggacatt
     8761 ttctggtgcc ccaaagcctt cgccagtcac aagcgctacc cctgtttctt ctaaaagatc
     8821 ttctacccat tgtgtgacat tttcatactt acacatgcgt agcgtttctt ttacattagg
     8881 aaaaagatag aaagctcctt gcggcttttc aagagaaaca cccggcagtt ctgccacaag
     8941 cggatataac gtattcaggc gttcttcaaa ggcgttccgc atttcttcta ctgtatcttg
     9001 ctcacctttc aatgcttcaa cagctgcata ttgactgact gctgtcggat tgctagttga
     9061 ttgggaagca acagcgatca ttccattgat gatcacagga tttcccaccg cataaccaat
     9121 gcgccagcct gtcatcgcat atgttttaga cacgccgttg atgatgatgg tctgattttt
     9181 gattgcttct gaaatcgttg caattggagt aaactcattg ccattataaa ctaaacggcc
     9241 ataaatatca tcagcaacta tcaaaatatc attttttact gcccattcac cgattgcctg
     9301 cagttcattc ttgctataaa tcattccagt cggattcgat ggagaattga tgattacggc
     9361 ttttgttttt tctgaacgtg cttgttctag ctgttcaaca gtaactttcc agctattgga
     9421 ttcttcccct ttcacaaaga caggaagtcc ttctgccagt tttacttgct caccgtaact
     9481 gacccagtaa ggcacaggaa tgatgacttc atcttgcggg tcaaggatcg tttggaaaag
     9541 ggtatacaaa gcaaatttcg ctccgtctgt tacgactgtt tgtgaaggtt catagacaag
     9601 tccatagttt ttcttcagat aatcaacgat tgcctgtcga agctctggga ttcctgctgt
     9661 tggtgtataa tagctagctt tcccattgcg gattgcttcg attgctgctt cttggatgtt
     9721 ttctggtgtc gcaaaatccg gttctcccac agtcaaactc agcacatctc tgccttttgc
     9781 ttttaatgcc ttggcctttg ccgcagctgc caaagtgaca gacggttcta atcgttttgt
     9841 tctttttgat aactccatcc cattcattcc tttacctttt aaattccctt gattgtcttg
     9901 acttcatctc cagattcaaa gccaatcagg taataattca gtttcccatt tgtttctttt
     9961 cctactactt cccacacagg ctgatcatca tacatcccta aagcagcttt ttcgatttgg
    10021 atttcaggat gggccgcagc aattgtctgt tttgcttcgt tttcagtcag tccctttgtt
    10081 tgatccaata cttttacttt ttcaccagat ttaggaatga taacggcgat ttcttttcct
    10141 ttatcatttg tacctgtcag tgaaaagtat gtcttctttc gagtaaacca ataaaaatga
    10201 tcggtttcct caagatttgc atatttcttt gcgatttcta ctgcttcttt cttcgcctgc
    10261 accgccggac gactggaacg caagtaaaaa atcgagctga tcacgatgac cgctaataaa
    10321 aatacgatca agccaagtaa cacgcgatta atcgtttttt cctttgtctg ttttttcttc
    10381 aagggtcgtc gctcctttat cttcattcct tttcattata gcagatttta attatgaaaa
    10441 aagacagctc ctctaacttc cttcttcatt tttctttaag aattcatgga tatcctgata
    10501 gatttcctcc atagctagct ctttgatcgg tacttttttc ggtaatgcat ggatgatccg
    10561 tgcgccatat tttgcagtga ctagtcttcg atccaaaata agcaatacgc cacgatctgt
    10621 ctccgtcctt atcaagcgac ctaatgcttg gcgtaacttc aaagctgctt taggtacgga
    10681 ttcctggtaa aaaggattga tcccctctga ttctaaataa gcgttcctag ctttgaccat
    10741 cgggcgaagc ggattatcga agggtaaacg tgtgactaca acgatctgca aagcatctcc
    10801 aggcaaatct acaccttccc aaaagctgtc agcgccaaac aatacgcttt tttctgacaa
    10861 aatgaagcgt ttcaaaagct tctcccggct tccaccaaac ccttgagcta gtatttctcg
    10921 tcctttttct agcatagaca gatgcatttt ctgatagacc ttctgcaaaa catcatgaga
    10981 agtgaataac acaagtattg gccgatcttc ttgttcgatc agacctgtaa gtgctgtctc
    11041 taaataatcg atatatgctt gattgctgac ttgatggacg gcaggtgcct cttttggcac
    11101 atagatcctt gcctgtttct cataatcata aggagaggca atcactttaa gaggtgctgc
    11161 ttcaatgcca agcctcttag agagatagtg tcgattaggg ccgatcttca gcgttcctcc
    11221 aatataaaga atacgatcat atcgaggata ccaaacagtc gacggcaaca tagccgcttg
    11281 aaaatcatgg agatgaaaaa tcatctgctg cgctgtgttc ccaggaacga accaatggat
    11341 caaatgatct cgccagtctt ccagccattg atccatcagc aagtattgct tgtctaattg
    11401 atcaaatagg ctatataaac ttgctaaatc gatccgcatc tggtttgtcc agctttcttg
    11461 gataatctca aaagtcagac gcatttgtga ctgtagctgc tggatctcct gataaaaaag
    11521 ctgcaggtga tcaatggctg tcccagattc cagtggcagc tgatccaaca tctcttttgt
    11581 catcatgact tcttctggat actgtccgtt aggttgcttg ccgctcacta aactcattaa
    11641 tccttctgcc atacaaatct gtgcttcgca tagcgcagaa agttcttctc gataaatcga
    11701 taaccagcgt aaggcttcat gttctttcgg taaatagctt tgtattcggt caaacagctg
    11761 atttggttct tctaattgct ggatttgttt atggaaatag ttaatactta atcgtttgtc
    11821 atttactttt tcagcgattt ccggcaaatg atgtacttca tcgatcaaca aaaatggact
    11881 agctggaagc aatggttctt ttcgctgtgt ttcttgtact aaaaacgcat gattgacaat
    11941 caaaaaatta ctttgcctga ttttcttttg caagaaacga acaaagtctt gttcgtaaaa
    12001 tgactgattt cttgctaaaa atccaatacc tctgtgcgcg atatctgcaa agaatgaatg
    12061 gttcaaattt gttaaatgta attcatccaa atcaccagtc ttcgtttgcg tcagccaaac
    12121 taagaccgcc atttgataca aagcatattg tttttgttct ggcggttcca caagtgtgcc
    12181 tttaaaacgc tgcaaatcaa tatagtggcg atagcttttg ataattacag gttgtatggg
    12241 ctgatctagc aattgattca gcaatggaat gtctttttct atgatctgct gctgcaaaac
    12301 tagcgaaacg gtactgatga cagcgggttt ttcaggtgta gccaaataac tcaatggcag
    12361 cagataaccg attgttttac ccattcctgt agctgcttcg atcatcaaat cttttgattc
    12421 atcttttgtg aaatggtcgt agaccgcatt catcagtctg ttctgttctt ttcgatacat
    12481 cagtgtttta ccaaacattt tttcttttgc ttgttttttt cttggatatg tccgctcgcc
    12541 ataatggacg gatgtgaata gttccacttc ttttttctgc aaagctagcc catcgacgat
    12601 ttctaatgag ggatcaagtg gctctggttt ttctttcatt tcttcaatca catgataaat
    12661 gaaacgactc gtatccatac ctgtgtgtct acttaatcgc gcaatctgct ctaacgtgat
    12721 gattggcaat tttctcatcc gttcttcgat cagcaaaaga agttgacctg tcacttcggc
    12781 atcactatct gcctgatgag gattctcatg aataaatcca aggctttctg acaaatcagc
    12841 taagcgaaag cttggttccg tcggcaagaa aatttgtgca agttctactg tatcgatgcc
    12901 tggtatcttc aaactaggaa gtccgcatcg catgagttca tggttcaaaa aattataatc
    12961 aaaataaata ttatgcgcga caaatatggt gtctgccaaa agattgtaaa tcgtttgggc
    13021 gacatcttcg aaataaggcg ctttttggac gcgttgattc gtgatccctg ttaaatgctg
    13081 gatttgtttt gaaatcttcc gccctggatg aatatcgatc gaaaaacgtg aagtgatccg
    13141 acctgattca atcatgacgc agccaaattg gatgatccga tcttcttttg gattcgtccc
    13201 agtcgtttcg atatcaacga ctgcataagt tcttggtttc aaaatatctt cctcttctcc
    13261 tgcctctaat gttcttttga aattatacta caaagtgcag aaaaatactc tcatcgcttt
    13321 ttaaagaaaa tagacaaaaa cgcccagaca cgcaaatgtc tgggcgttta ttttttagta
    13381 taggttaatt aagctttagt aacgtttgtt gcttgaggtc cgcgttggcc ttcttcaaca
    13441 tcgaaagtta ctgcttggcc ttcttctaaa gttttgaatc cgtcgccttg gatagctgag
    13501 aaatgaacga atacatcgtt tccgttttct gcagtgatga atccaaatcc tttttctgag
    13561 ttaaaccatt ttactgtacc tgtttccata ataaaaatcc tccttgtgta tcaacacata
    13621 ttttgcaatt acttgaatgg tgtatatctg gaagatgttt atttgaaaca agctaccaca
    13681 gattaccgaa caaaaactac tcctatagta tatcataccg tttacaaaaa agctagagtt
    13741 tttcgacttt ttttattaaa aaaagttttc tcagcgatta cataaagtga aaaaccgaac
    13801 ttttttttaa aataaacgac atattgttca gttggtgttt ttttgctctg atgttctttt
    13861 ttaaactaaa aaaattattc tttctgacta cttatcggtt ctgtttcaac ggtcgaggct
    13921 ggggaagatt ctttagtagg tctgatgttc tcatggtatt tctgatagcc tatgacagtt
    13981 accagaagaa caaggcaaac caatacgacc aacgagatta gtttttcttt tttgctcata
    14041 tctatacctc ataaataaca ggatgatgtc catcatattt ctgagtatga gtaaaagcaa
    14101 aagctgcagc tgtagaatgt aagaaatgcc agcaactttc ataatcacag gtcggacagt
    14161 accaaaagta accaaatctt ggctgcaaag accgttcttc tgtttcagga atcgcagaat
    14221 caagatctgg tgtctgtaca taaacatttt tgtacagacg tgtgcctgta ataggaaacc
    14281 gctgtgtttc ttcagcaaaa actggctgac ttgttaaact tagggataga gacagcaaac
    14341 accataacag tctttttttc ataaagacct ccttatcgat aagtttttcc caattaaaat
    14401 catatctcaa gaacgcccgt tattcacatt ttgttcataa atgatgtttt ttgtatcata
    14461 atcgtcaaaa agaggtcgtg accacgtcac aacctctttt ttaaacggtg ttccagaagt
    14521 agcttcttcg gaaataagcc gaaatttcac aaaaatttga gaaataattt ttgtcaatct
    14581 cttcttattt cttgaagctg accacttctg tcacaacctc ttccttaaac ggtgttcaaa
    14641 agcttgcatc tgcggtaata agcccaccaa acaaaaaata tgaagagcta tttttgcttg
    14701 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctcatacac ggtgtttaat
    14761 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gtgaaactat ttttccgttt
    14821 gtttctctaa tactcaggtc aaaacactga tttcacaacc tcttttaata acgaagaatc
    14881 gctgtcagac tcttttcatc tggcgcatct ttttgatcca aaataaacac ttttccgcca
    14941 ttaatgatca catcatctgc taatgcattc aacacttgac gccgatcata ttctgtttca
    15001 ggatcttcac cgaaaccatc agctaagttt gacgtagcga taaataaatg tgacactttt
    15061 ccttctttcg cagctggaac gatatcaact aactgatcga tgaatttctt atctaagagt
    15121 ttattgtatt cttttgattc ttgagctgcg agttcacttg acagcttctt cgccccttct
    15181 tcgatatctc taaaggatag ctgcgcagga gaagcagaaa tcgcaacgtc tgcacaataa
    15241 tagggatttt tagcaatttt tttgaacatc gtctgatttt ctggtagagc aaataaataa
    15301 atcggccatt gttctggatt ttcgaaagaa tccttcaagt acgtatcaac tgcttggtaa
    15361 tagttgaccc aatcgatctc tacttcttca tcttttggat ttacgccatg gaaagttact
    15421 ccatctcggg ctccgcctcc tccattagaa gagaagttca aaccgccacc tgtcagttca
    15481 gatcccaatg cagtaacgac atcaaccggt gccccttctg gtagagatac ttcttttact
    15541 tgtttgttgc tgacttcata caatttcatg gagtcgcggt tcaatcccaa taaataataa
    15601 gaatagttga actgcccatt tttgataata gccagtaaat aaggaagatc acttacatag
    15661 tattgatcgt ctaccgggat actcaaacga tggatatagg tttggtcagc agttataatg
    15721 accgctacac tcgtagttgc actacgccaa aacgaaggat ctcctaacaa attttcaatt
    15781 ttttcttgga acggggacca gtcatgttcg gaatattttt tctcaaaacg tttctttgct
    15841 tctttcgcaa aatttttgaa tttgatctga tctttttcca catcttgatg ggcgacatgc
    15901 gtattcagca tgatcgtgac gaatggtcct ttagcgcctt ctgaagtcaa ttgtgacaat
    15961 aattctttat ccttttttgc cataaacgta cccctcctaa aaaaatcatt cctttgtttc
    16021 tatctaaagt ctaccacaac actatttttc catacaataa aacgtatcta aaaaaataaa
    16081 acggtttcta cggttttaaa tttagctatt tttttaagtt atcttcataa tttcctcaat
    16141 tatttagccc cagtcttcaa ctattcttca tgaaatgctg ataaactaag aaaaagaata
    16201 aaagaggtgg atcttatgca tttgattatg attttcagtt tattaagtat tatcagttta
    16261 tttgctttca gcgcttcccg cttattagat gcggaaacac agcctattcc tgtaaaagta
    16321 cgtaaaaacg accgctaacc ttccataacg tgcgttctta tcctaaagtt ttttcagaaa
    16381 cattgaaact ctctcccctc tctgataaaa ttttaatttg tagcgaaaca aattaattac
    16441 aaggagatcg gatatgtcca taaaaaaaac acgtctagtc ctattattgg ctagtctcat
    16501 tctttgcctt gcttcttttc taccagctgt tgctgtgcaa gctgaagata ctttcaaagt
    16561 aaatgcaaaa gcagcctttg ctgtagatgc gcaaagcggt aagattcttt atgatcaaga
    16621 tggagaaaaa accatgggga tcgcttcaat cacaaaaatc atcggtcttt acatcgtcct
    16681 tgatcaagta aaagaaggga aactatcatg ggatgataaa gtttcaattt cagattatgc
    16741 agaaaatttg agtgtcaccc cagatttatc aaatgttcct ttgcataaag aaaacaccta
    16801 caccgttaaa gaattattcg actctgcaat catccagtca gccaacgctt caatggttgc
    16861 cttggcagaa aaaatctctg gcagcgaagc aaaattcact gaacgaatga aagaacaatt
    16921 gaaagattgg ggtatcaaag atgcaacgat cgttaatgca tcaggactga ataactctta
    16981 tcttggcgaa aaccgaccag aaggtactgg agaaaacgac gaaaatcaaa tgtctgctca
    17041 agatgtggct atcgttgccc gtcatttgat cttggatttt ccagaaatac tggatgtatc
    17101 tagcactacg acacaaatgt ttggcgaaaa cacgcaatca ccagtagaaa tggtcaactg
    17161 gaactggatg ctcccaggat tcgtgaatta taaagaaggc gtggacggat taaaaactgg
    17221 tacgactgaa cttgcaggag cctgtttcgt cggtacgatc actaaaaacg atcaacgaat
    17281 catcactgtc gtattgaatg cagacggcca tgcggaaaac ccaagtgccc gtttcaatga
    17341 aacagctcgc ttaatggatt attgctacga caattggtca gaaaaagagc ttggaaaagc
    17401 caatgcaagt atccctagtc taaaaaccat tgatgtaaaa gacggaaaag aatcaacagt
    17461 gcccgttgtg cttaaaagtc ctgtaaaagt atgggtacgg aacgatatgg atactggaaa
    17521 attaacaatc acaccaactc ttgacaaaaa acaagtcact gacaatgaga tccaagcacc
    17581 tatggataaa ggaacaaaaa tcggtaccgc aacgatcacc ttggcagaag ataaattagg
    17641 ttatcttgaa gacaacaacg cgccatccac agaaatcatc acggataaaa ccgtagaaaa
    17701 agcaaatatt ttcgttattg gctggcgacg cgtcgcagaa tttttcggta acttgtttta
    17761 attgaacaaa aagaaaaaag catctttcca tgtggaagat gtttttttgc tttttctagt
    17821 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    17881 tatgaatcta accagagatc tgattttttt ggttctctat cttattgggg tattctccat
    17941 cgtttattgg cgaaaacaga aaaaggatcc ttccttgttc ttttacagca gtacatggac
    18001 gtttgcggta atcttgactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    18061 tttgattccc ttagtgtttt tcagtatctt tgccttttcc tatttttcag aaaagcgaag
    18121 gctgttgaat ggtcttttat tcaatatttt cctcattagt tttggtatct acttgtttgt
    18181 cttgctatac gaaacacaaa atatttttct tggcggactg attgccttga tcacgatccc
    18241 gttactgctt gtctttctat tcggcattta tggattgatc gtttttcttt tttggaacgg
    18301 tgtcacagtt ttacgtagag agtcgcattc attagctaat cttttaacgt tgatactagc
    18361 catttttctt acattgtttc ttgtttttga ctttttcttg ttaaaatatt taccacaatg
    18421 gataaatgct ttgtttttct gcatcccatt gatcttgatc tatctattta tcgtattcta
    18481 caactttttg actgtctcat tcttgtatca gttcaaccgc cctcgataca accaagactt
    18541 catcgttgtg ttaggcgccg ggttgatcaa tggagaaaca gtttctcctc ttcttgccaa
    18601 gcgaatcaat aaggcgattg ctttttattg ggcacaaagc cgtgcgacat taaatccgcc
    18661 tatcctatta atgtcgggtg gacagggagc agacgaaaaa gtaccagaag caatggcgat
    18721 gaaacaatat gccatggaac aaggaattcc tgaaagagat attcttgtcg aaaccaattc
    18781 gacaaccact ttggagaata tgcgctattc aaaggaaatc atggatcagc aaatgaaagg
    18841 tccctatcgc gcgatcttca gttccaacaa ttaccacatt ttcagagctg gtctctatgc
    18901 acgacaagcg aaactgaagg caaatggcat tggggcaaaa acagctttct attacttgcc
    18961 taacgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    19021 catttgcgga ttgcttttcg caggttccgc attcttatca ctgatcactt tacttttcta
    19081 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac
    19141 tattggcaaa aagagtatcc cttttctgac cgctacttgc tgagcagaat aaataccgct
    19201 gttgcctgaa cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga
    19261 accgaacaat tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa
    19321 tactccgata aagcccaatc ctgcaagaaa aggaacagaa acatggagca aaggcgctag
    19381 tgctgcacca agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc
    19441 cgcgcctaaa gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg
    19501 tccattaaat gattcctgca gtaatggaag actcaatccg agataacgtt gtgtttgcaa
    19561 aatcaatgct gcggctacaa ctacagcccc acctatcgta tttcgccaaa caggatgagg
    19621 aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
    19681 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
    19741 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca taaaatttgc
    19801 aaacagtgca gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa
    19861 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
    19921 actgattcca ctgataatca agatttcccg ttctgattta tccaatcgga acaaacgtgc
    19981 tgtattatct gctaacgctc cccccatttg gactgctgtt ccttcacggc caacagatcc
    20041 tccaaataaa tgggtagtta tggttccaaa cagtgtaagt ggaataagtc gcaacgggat
    20101 tttttcttct ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagacc
    20161 gccgtgatac gcatagagat aagcaaaaag cgcaccacta actggtagta gataaaggag
    20221 ccaaggatgt gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga
    20281 taagctcccc atgaatatac cgagaatcgc tgtgatcacc agccacttca gcatgtacac
    20341 taaaatgcgt actggactcg gtaattgttt ttccataaaa aattcctcct caataaaaa
//
LOCUS       DACCXW010000047        20388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCXW010000047 DACCXW010000000
VERSION     DACCXW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.047"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            69..3458
                     /locus_tag="IVY71_001397"
     CDS             69..3458
                     /locus_tag="IVY71_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7985458.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3461..4882
                     /locus_tag="IVY71_001398"
     CDS             3461..4882
                     /locus_tag="IVY71_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7985459.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4879..6756
                     /locus_tag="IVY71_001399"
     CDS             4879..6756
                     /locus_tag="IVY71_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7985460.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6848..7690
                     /locus_tag="IVY71_001400"
     CDS             6848..7690
                     /locus_tag="IVY71_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7985461.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7842..8717)
                     /locus_tag="IVY71_001401"
     CDS             complement(7842..8717)
                     /locus_tag="IVY71_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7985462.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8924..9955
                     /locus_tag="IVY71_001402"
     CDS             8924..9955
                     /locus_tag="IVY71_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7985463.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10044..10349
                     /locus_tag="IVY71_001403"
     CDS             10044..10349
                     /locus_tag="IVY71_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7985464.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10528..12543
                     /locus_tag="IVY71_001404"
     CDS             10528..12543
                     /locus_tag="IVY71_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7985465.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12545..12829
                     /locus_tag="IVY71_001405"
     CDS             12545..12829
                     /locus_tag="IVY71_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7985466.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12855..14228
                     /locus_tag="IVY71_001406"
     CDS             12855..14228
                     /locus_tag="IVY71_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7985467.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14311..16326)
                     /locus_tag="IVY71_001407"
     CDS             complement(14311..16326)
                     /locus_tag="IVY71_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7985468.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16348..17562)
                     /locus_tag="IVY71_001408"
     CDS             complement(16348..17562)
                     /locus_tag="IVY71_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7985469.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17783..18727
                     /locus_tag="IVY71_001409"
     CDS             17783..18727
                     /locus_tag="IVY71_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7985470.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19023..19967
                     /locus_tag="IVY71_001410"
     CDS             19023..19967
                     /locus_tag="IVY71_001410"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7985471.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6967 a         3733 c         3882 g         5806 t
ORIGIN      
        1 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
       61 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
      121 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
      181 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
      241 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
      301 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
      361 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
      421 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
      481 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
      541 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
      601 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
      661 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
      721 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
      781 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
      841 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
      901 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
      961 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     1021 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     1081 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     1141 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     1201 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     1261 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     1321 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     1381 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     1441 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     1501 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     1561 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     1621 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     1681 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     1741 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     1801 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     1861 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     1921 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     1981 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     2041 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     2101 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     2161 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     2221 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     2281 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     2341 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     2401 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     2461 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     2521 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     2581 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     2641 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     2701 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     2761 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     2821 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     2881 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     2941 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     3001 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     3061 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     3121 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     3181 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     3241 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     3301 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     3361 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     3421 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     3481 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     3541 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     3601 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     3661 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     3721 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     3781 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     3841 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     3901 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     3961 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     4021 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     4081 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     4141 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     4201 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     4261 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     4321 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     4381 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     4441 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     4501 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     4561 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     4621 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     4681 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     4741 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     4801 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     4861 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     4921 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     4981 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     5041 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     5101 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     5161 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     5221 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     5281 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     5341 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     5401 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     5461 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     5521 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     5581 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     5641 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     5701 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     5761 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     5821 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     5881 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     5941 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     6001 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     6061 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     6121 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     6181 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     6241 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     6301 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     6361 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     6421 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     6481 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     6541 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     6601 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     6661 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     6721 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     6781 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     6841 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     6901 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     6961 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     7021 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     7081 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     7141 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     7201 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     7261 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     7321 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     7381 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     7441 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     7501 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     7561 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     7621 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     7681 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     7741 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     7801 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     7861 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     7921 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     7981 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     8041 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     8101 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     8161 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     8221 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     8281 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     8341 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
     8401 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
     8461 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
     8521 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
     8581 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
     8641 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
     8701 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
     8761 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca aaccgaatga
     8821 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
     8881 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
     8941 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
     9001 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
     9061 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
     9121 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
     9181 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
     9241 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
     9301 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
     9361 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
     9421 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
     9481 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
     9541 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
     9601 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aaagggatgt tcgtgaaaca
     9661 aacaaaagat cgacaagaag aacatctgaa aatgttttac atgcctgatc atgaagactt
     9721 tggtatcgat ttgcccgaac attatggtgt attgacctat atagatgaac aagggagtta
     9781 tcatgaagaa aaagtaattt ccgaggttgg tgattacagc cgtgtctacg aagggattta
     9841 cgagacatta gttaatggcg cagaaaaagt catcaaagat gaagaaacaa tcttacaaat
     9901 gaaaattttg gaagaaggaa tcaaaagcat tcaagaatca gaaggaaaaa tatgacatat
     9961 ttgtaagaat cgggtttgtt ttttgacgca taaactgtct tattgtacga tgaaaaagta
    10021 tagaactact aggaggaacc aacatgacaa attatgacgg tgaaaaacac aaacgattaa
    10081 gaggttcaat ggcccctaaa acagaagaag aagtggcttt agcaggaact aattctggtg
    10141 aaacagaaga caaagaagaa tcattagctg gcacaaactc tggtgaaaca gaaaataaag
    10201 aaacagcttt atctggttca aattctgtag aagaggaaac aaagcctaat gagcccaatc
    10261 actttgaaaa agatatcgaa aaagcggttg aagatgtcaa agaaaaattt gaagaactga
    10321 cctcaaaaaa taaacatgat catcattgat cggacgaaat caattcataa aagcaggaga
    10381 catttggtca cctgcttttt ttctgtatcc attcgataga aatcgttcgg tacagtcgta
    10441 aaaaaaatcc aactgtaagt tgtaccaaac acccctcgat ttaaaagcgc taccatacta
    10501 gaatataagt aggaggtgaa cagcgctgtg aactacaaaa ttagttatct cctgaaaaca
    10561 ccaagagaaa tcagtctaga aaaagcagca gagatcatcg aagtaaatac aagcgaggcg
    10621 atcaaacaat taactccttt tgccgaatat gtggatgata cccgtatccg tataaagaaa
    10681 ttccgagcaa tcgactgggc agaaaaattt ttggatatta ctcgatttga agctgtgtat
    10741 accgaacaag agcgaagaga tcttctttat ttgatggttt attcgactgc aacgtctgtt
    10801 tctgtttttc attttcaaga ttttcttcag gtttctaaag ggacagtcct gtcagatatc
    10861 aaaaagataa gaaatgaatt agaagtggaa gattgtcagc tgtcgtatac taggaaaact
    10921 ggttttaggc tgctagggtc agaagcaata ttaagaagag aagcaaaaaa tcgcactgct
    10981 cgtttgatgc agtcacttgc tggaagattt gggctcacgt atttaaccgc aaccctttcc
    11041 attgattatt acgccaaagt tagagatttg ctagataaaa cgatttcact atcagatctt
    11101 cgattcgcac caaatcgaat ggatgaatta gcttattatt tgagtttgac aaccattcga
    11161 attggaaaaa catccatgga actggtaaaa gatgctgaac tgttggcttc tcttgcagtc
    11221 actgacatta gtcgacaagt attgactggc cttggctatt ccgaacatca aaattcggag
    11281 attatatttt tctctctttg cttagcatcg atcgttgaag gagatgtcca ggagcctgca
    11341 cttgattttt tgctggaatg ttcttcagaa gtcatccatc gcatggaatt cctgatggcc
    11401 gtagaatttg cttcgtttcg tgaattattg atgaatgtct tttatcactt ggtccctgcc
    11461 tattttcgta tttcctattc tttttattta cctaatgtga tgatcgatca aattaagcat
    11521 caatatgctt ccatctacga aatgacaaga aaagcattac ttccgttaga aagacgaata
    11581 gggaagagca tccctgaaga agagattggt tttttcacta tactatttgg cggagagatc
    11641 agaaaagcgg acgcagaaga aagaaataga aaaataagag ccgtgatcgt ctgtccaagt
    11701 ggcattagct cttcattgat cttaaaaagt gaacttcagc aattattccc gatgatcttt
    11761 tttacagaga ctaattcagc tgaccgctta cacgaggttg acgaaacttc ttacgatatt
    11821 attttttcta ctgtacctct cacacttact gataagaaaa agtctctcta tgttctgcat
    11881 ccaatcatga ctgacttgga aaaaaatcag ctgatcaatc aagtacaaag agattggctg
    11941 ataccaggat tttcaatgcc ttctgcccaa gaattgttgt cggcgctact gccttatatc
    12001 gaattaaaag aaggcgtaga cgaagaaaag ctctatcaag tattaaatcg taaaatgaac
    12061 aaacttttag aaaagaagga ggatcaacgt cctatgctat ctgaattatt aacagaagaa
    12121 atgatccaat tatcggatca accaaaaaac tggcaagaag caatcacatt tgccgcacag
    12181 ccgctattat cagaaaataa aatagaagaa agttatatca aagctatgat cgagcgcgtg
    12241 gagcagtatg gtgcgtttat ccatattggc gatcacatcg cattacctca tgctcgtcca
    12301 gaagatggcg taaacgaagt agggatgtct ttgctgaaac ttcaacaacc tgtggatctg
    12361 cttgataatc cagcccatcc cattagtttg ttcatctgtt tagctgctat cgataatgag
    12421 gcacatcttc gggctttggc aaatttaaca aagctacttt ctaataaaca aaatcttcaa
    12481 gatttattac acgcatcaac aaaagaagaa atattagcga tcataagaaa aggagacgaa
    12541 tgatatgaaa atttttgcag tatgtcaaag tggattagga acaagtttta tggtagagat
    12601 gaacatcaaa caagcacttg cagatcttgg agcagatgtg gacgagtttg aagtaagtca
    12661 tacggatgtt ggaagtgtat ctagcgatat ggctgattat ttcttcattg agcattcttt
    12721 gaaagatacg ctaacttcta tacctgatga aaagctcgta cctttgcaat cgatcattga
    12781 cagcgaagaa gtaaaagaaa aagtaacgaa gatcttgtca aaagaataaa aatgattcaa
    12841 gggaggaact atcaatggat aaattgttga atttgtttgt gtcaatcgtc agtcagccct
    12901 ccatcctcgt ttctttgatt gcactgcttg gtctcgttct tcagaagaag aaattttcag
    12961 aagtgatcca aggaactatc aaaacatttg tcggattcct agtactgatc ggcggtgctg
    13021 gtttaatcag taattcacta accccttttg cttcgatgtt ccaaacagct ctgcatactc
    13081 aaggagtagt tcctagcaat gaagcagtag tggcgattgc tttgcaacaa tacggtacac
    13141 ctactgcttt gatcatgcta gtagggatga tcgtgaatat tttacttgct agattcacaa
    13201 gatataaatt catttttctt acaggtcaag caatgcttta tgtttcttgt ctaacagcag
    13261 tcattttagt aagcgcgggg tttactgcaa atttgtggat gatcttatta ggcggggtct
    13321 tcgaaggaac actattaact attacgcctg ctctttgtca accgtttatg aagaacatca
    13381 ctggaaacaa tcaagttgcg ttagggcata ccggaaatat cggttatgct tctagtggat
    13441 ggatcggaaa gttttttgga aacaaagacc gttctacaga ggacataaag attcctaaaa
    13501 gtttcagctt tttgcgagat tctactgtat cgatcatgct cttgatgtcg atcgtatatg
    13561 tcgcacttgc tttgttagct ggtcctggat ttgttgaaag agaactaagc aatggagaca
    13621 acgctgtaat ctttgcactg atccaagctg gaacattcac agctgggttt gttatcgtcc
    13681 ttcaaggggt tcgaatggtg ttggcagaaa tcgttccagc attccaaggg attgctaaaa
    13741 aactcgtacc cgattctaaa ccagcattag atgttcctat cgtttttcca tatgcaccta
    13801 atgctgtatt aattggcttt tttgttagct tctttgtagg aacactatcc atgtttgcta
    13861 tgataggctt gcaaactaca gttatcattc ctggggtagt aggacatttc ttctgcggag
    13921 ctgcttctgg tgtcttcggc aactcgactg gcggtagaag aggggcggta ttaggttctg
    13981 ctttcaatag cttattgatt agttggctgc cattatttat ccttccagta ttaggagatc
    14041 ttaaaatcgc ttcttctaca tttgccgata ctgattatct agtaccagga cttgtacttg
    14101 ggaaactagg agaatacggt caaatagcct tgacagttgg aatcatcgct ttctttttac
    14161 ttgtcgtatt atctagcttt ctttttgccg gaaaaagaac aaacattgtt tctaaagaac
    14221 agaattaagc aaacttcaaa ataaaaattg cttggacaat atcagaagaa gatttaaagt
    14281 cttctgatat gtcgcaagca gttttttgtt ttatgcattt atttttgaat agcttgttaa
    14341 gacaagtggc tgttttgcta gaagaggata acttttacca ttcattttca ctgttccgcc
    14401 tgttctatca gaagtaatca cgatcttatc ttttgttttt tcaagcgaaa tctttccatc
    14461 tttgataggt agcgtacaat tcagtgtttc aaatgaatct agattaggtt gtatcataaa
    14521 ttcttcataa cctggctttg ttggatacaa accgataaaa tagcgtccta aaagatagat
    14581 tgggcttgct ccccatgcat ggcataagct ttttccataa ggatctccat acatactgta
    14641 cttttcattt cctgtttgag tagggtcgta ttcttcccag aaagttaccg catcatgaga
    14701 taacatccct ccccaataat caagaatcgt ttggtagacg atattcgtct gtccgagttt
    14761 gcataaagca tcttgttcat agaatttgaa atatggagta gtaatctgtg tgatagtgtc
    14821 attcaataag acattcttta agatcgattg ctgttggtct ttgttagcaa aatcgaataa
    14881 aacagcgaaa atattcgcat gacgcgtcac atttcttctc ccagattcat agcaatcgat
    14941 aaaagcgcca agttcttcat cccaaaagta gtcaaatagg ttttgtttca atagttgata
    15001 tttttcttga tagagatgag tatgataatt ttcgttctct ccatatgttt cagctaagat
    15061 atctccgcaa gtgatcatgg aatgataggc tttcagtaat aagacttgtt ctgcagcaag
    15121 cgttccttct ttatccattt cagaccaatc aataaatatc caatcttttg ctctcccata
    15181 aataaaccct aattcattcg tttgctccat caagtatctc attaatgatt gcatcttagg
    15241 gtagacgatg cgtaaaaatt catagtcttt cgtcatcata tagtggtttt cgataccaat
    15301 caaccacaag atcgaatagt caacgatagt attcatatgc tgcttgattt cattttgtcc
    15361 tcttaatgct aatatcgttc gtttattgat ttcctcgtca aaaaaaagat attgattgat
    15421 catgaatgat tgatacgcat ctccagacca aatccagcga tctcgtttga taccgtcgat
    15481 aaaaaacaaa tcactacata acttgaaggc ttcttctgat actgaccaga tctgattcag
    15541 taaagaagaa tcgcttgaaa aagcagcttg tttcgaaaga gataaaaatt catgaaaaac
    15601 ttgaagttga accattccag tagaaacctc tggtataaac aaatagcgaa aagcgcgttt
    15661 acgagctttc atttttcttg tcattcctat ttgcttatag tagcaataat cgatatccat
    15721 agcttctaca tcggactctc cataacatag agtgatagat tcgatatggt catccagaga
    15781 tattagttct acacagccat tcactgctcg cccaaaatcg aataagattc ctccgccaat
    15841 ctccgtttca gtgactggat ggacgcattc tttttgataa tagatttgat ttggatcttt
    15901 tgcgatatcg acataaagcg gactccagcc agctggatat tcttcaataa aattacttgc
    15961 tgtccagcca atatctgatt ttacttcgtc tccctcgata aacatcgctg gtagccctga
    16021 tgtatgtcct acaaagatac gaatttttgc tttaccagct ggacaagtca gccattttcc
    16081 acatggatgt ttttgtccat taatttctac atacccaact ccttggatcg tcactttaaa
    16141 catggaaggt tgatcaagaa agtaatatct cttaaacttg acgttccgat ggcaatcatc
    16201 catgtaccag tacgctggcc agtcaaaacc tcgttcttcc cgttggaaat tttgtagcag
    16261 tccatggcgg atctcaaagt ctccaggata ccaaagccat ctatctttat gtttttcatt
    16321 tgtcatttta cacgacctcc atcaaattta ttttgttgtc aattgttctt ttatttcttc
    16381 atctactctt atcactgtct ttctattcgt cctgtgtact actagaaggc taataagcgc
    16441 gctaatcaac agcaagatac tgccattgac gaaaacagca agtgttttac tcccaagaga
    16501 agcaaaagag agaaagagca aaccatatgg tgcaatgaaa gtacctaaat tagcgccaac
    16561 caataagatt gaagtattga aactctccgc gtacttgggc gcttggatac ttacttggtt
    16621 gaataagtaa ggaatgaata acgaaaaaga aattcctgtt aggatcgcac caaacccaat
    16681 cacatacagg tttatcacag tggcaatcaa tacaaagccg attcccatca gtagtaaaga
    16741 aagtgtaagc atatagcgat gtgcttttgt ctgaagccag ccaaaacaaa ctccggcagc
    16801 catacttgcc aattgcatga ttgttaagat attcgctgca ttagtttcgt ttcctagaga
    16861 ataagtcgtc aggactgtgg ctaatttaat cgatacagca ttatatacca cgatgaccag
    16921 caagagaaaa ataccataac taatcatttt aggattcgtt ttgattactt gtcttgttgg
    16981 ggctgtgcca tgcgattgaa gttcagttgt ttcaggtgta ggaacaaaca aaacaaacaa
    17041 gacaagaata ggtaagctga tggcgtagac taaaaaggta tattgccaac cgaactgtac
    17101 tagctgtcct gcaacaaatg tcattaaagc tgcaccaaat ccttgaaatg cactttgaaa
    17161 accaatcata gaggctcgtt catctccgtg ataaaaggca gcaatcatgc tgactgccaa
    17221 agaattgatc aatccaaagc caatacctag tccagctctt gaagctaaga caatactgta
    17281 actttgtgag aagaggggga ggataccaca aatcgatgca atcactaatc cgatgatcac
    17341 agtttgtttt tgtccgattt tcctagcaat aaaactacta atcaaaacaa aaatagtgac
    17401 cataaaagag ggagcagtcg ttaataattc aacagcagat aaagaacgcg tggcataagt
    17461 agctgacata ctgggaattg cagacgtgac tgcaccagca gaagtcaaaa taagagagac
    17521 tgacagcaga gaaaatttca tcatcttttt attcataagc atgttccttt catttttgta
    17581 agcgcttatc aaaaatatag tatgaagtgg ttattctctc aatagcggaa tcacaggaag
    17641 ttcatgtccc aaccatagaa agtctttatg aaaccaaaaa tagctttgca tataatagca
    17701 gtacagcgtg aactaataag cgaaagaaac cgcttacaaa aaggagggag tcaattggaa
    17761 ctagctgaat tagacatttt ttttgcgtaa cgaagaatca atcgaaactc tgcagcgtga
    17821 acaaggaatg aatatcaatg atttaaactt agcttaccat cttcagacac cgccaaaaat
    17881 ccaccgaggg gatctatttc aacagggaaa tattcatatc agtaaacacc gtcgatatgc
    17941 ggaagttccc gagcatacac atgattttgt tgaaatcaat tatatgtata gcggtacatg
    18001 tttacaaatt attaatggag aaaggataga gatggaagaa agtgacgtgg tgatcattga
    18061 taaagaagct actcaaagaa tcgaatacac agggcaagag gatattttaa tcaacatctt
    18121 attaaaagaa gaaaccttat ctacagagat attgaatcat ttggcaagca caaataatct
    18181 actttcttct tttttgataa atgcatcaaa agaaaaaggg gaacatacac aattcattca
    18241 ttttcgcggc ggaaaaacaa gtcgtttgac gcatttgatt gaaaatatgc tgttgattta
    18301 ttttgataca tacagtcata agatccgttc gttaaatctc tatacatcac tattactgat
    18361 cgaattgact catttattag aacaacaaag cttttcatca gcagatggat gcgcagaaaa
    18421 tgagatgctt agagtattag attatatcga taagcatttt acttcttgta cgttaaaaga
    18481 aacagcagct cattttggct acaatccggt ctatcttagt aataaattga aaatgtattt
    18541 tgatgcttcc tttcaagaac tggttctaaa gaaaaaattc gactttgcgt tggaactgat
    18601 tcaagaaacg agtttttcgt tggaagaaat cgctaatcgt atcggatata aacaaacgac
    18661 ttctttattc aaattattca agaagtattc aacatgtact cccaatgaat ggagaaataa
    18721 acattaagaa gaaaatcttt cactaaagca tatgaaggaa aattgtttta ggtgcgattg
    18781 ctgtcttatt atatcgttgc agcgaatttt tttggattta ttttcagttc taaacttttg
    18841 gttgtattat caggggatat aggtgtaaac atttgtcatt tatctgttat gcgtattcag
    18901 gaattcgttt ccatcaaaaa ttataataac aactattata attaacatgt tgataaaatg
    18961 aataaacaag actatacttt tcatgaaagt aacaaatagt tatgaaaagg agctgtttat
    19021 tcatgaaaaa aacaagtcgt aaagtagtaa tcgtcggtac aggttttgtt ggaacaagta
    19081 tcgcttatgc aatgatcaac caaggaatct ctaatgaatt agtattgatc gatgtgaatc
    19141 aagaaaaagc agaaggggaa gcattggacc ttttagatgg tatggcttgg ggcgatgaaa
    19201 atgtggctgt atggtcaggt ggctatgaag aatgtaaaga tgctgatatc gttgttatca
    19261 cagctggtat caaccaaaaa cctggtcaat ctcgtttaga tttagttaaa acaaatgcgt
    19321 caatcatgcg ccaaatcgta aaagaaatca tgggatcagg attcgacggt attatcgttg
    19381 ttgcttcaaa cccagtagat attttgacat atatcgcttg gaacgaatct ggtctgccaa
    19441 catcacgtgt tatcggtact ggaacaacat tggatacaac tcgtttccgt aaagaaatcg
    19501 cattgaaatt aaaagttgac ccacgcagcg tccacggcta tatcttaggt gaacatggtg
    19561 attcagaagt tgctgcatgg tcacatacaa ctgtcggtgg taaaccagtg tttgaaattg
    19621 tagaaaaaga ccatcgtatt gcccaagatg aattagatgt gattgctgat aaagtccgta
    19681 atgctgctta tgagatcatt gatcgtaaaa aagcaactta ctatggtatt ggtatgagta
    19741 ctgcacgtat cgtaaaagct atcttgaata acgaacaagc tgtattgcct gtatctgctt
    19801 atttaactgg tgaatacgac gaaaaagata tctttacagg tgtgccatca atcgttgacg
    19861 aaaatggtgt tcgtgaagtt gttgaattat caatcaacga agaagaaaaa gcaatgttca
    19921 gcaaatcaac aagtgcttta agagaagtat tgaacactgt attataagaa acaatgaata
    19981 tgaactaaaa atcctaaaac tgcttaacag ctgaaatagc tgttaagcag ttttttattg
    20041 atatctaata ggtaagcaaa taaatagaat ggttaaaatt aatcggtccg aaaatgtatc
    20101 cgtacatatg aacatatgaa atttaatctg ttgattacaa taagcgaatc gttatcaatg
    20161 tttatctgtg gttaattaaa aaatccttcc gcaacggcaa atacaatgat tcatatctaa
    20221 ttcgttcata tttgattaac tgaataaaga aactggtttt tatcattcaa ttaaacgaca
    20281 aaaaaagtaa gaaaaactgc aaaatttttt tataattttg cataagcaga ttttgatgga
    20341 aaaatgacga tttttgataa aaaaccgttc gagagaggtt tatcattt
//
LOCUS       DACCXW010000048        20032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCXW010000048 DACCXW010000000
VERSION     DACCXW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.048"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            300..608
                     /gene="rpsJ"
                     /locus_tag="IVY71_001411"
     CDS             300..608
                     /gene="rpsJ"
                     /locus_tag="IVY71_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7985472.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            639..1268
                     /gene="rplC"
                     /locus_tag="IVY71_001412"
     CDS             639..1268
                     /gene="rplC"
                     /locus_tag="IVY71_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7985473.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1296..1919
                     /gene="rplD"
                     /locus_tag="IVY71_001413"
     CDS             1296..1919
                     /gene="rplD"
                     /locus_tag="IVY71_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7985474.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1919..2209
                     /gene="rplW"
                     /locus_tag="IVY71_001414"
     CDS             1919..2209
                     /gene="rplW"
                     /locus_tag="IVY71_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7985475.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2248..3081
                     /gene="rplB"
                     /locus_tag="IVY71_001415"
     CDS             2248..3081
                     /gene="rplB"
                     /locus_tag="IVY71_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7985476.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3124..3402
                     /gene="rpsS"
                     /locus_tag="IVY71_001416"
     CDS             3124..3402
                     /gene="rpsS"
                     /locus_tag="IVY71_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7985477.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3425..3772
                     /gene="rplV"
                     /locus_tag="IVY71_001417"
     CDS             3425..3772
                     /gene="rplV"
                     /locus_tag="IVY71_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7985478.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3786..4442
                     /gene="rpsC"
                     /locus_tag="IVY71_001418"
     CDS             3786..4442
                     /gene="rpsC"
                     /locus_tag="IVY71_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7985479.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4445..4879
                     /gene="rplP"
                     /locus_tag="IVY71_001419"
     CDS             4445..4879
                     /gene="rplP"
                     /locus_tag="IVY71_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7985480.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4869..5057
                     /gene="rpmC"
                     /locus_tag="IVY71_001420"
     CDS             4869..5057
                     /gene="rpmC"
                     /locus_tag="IVY71_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7985481.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5082..5348
                     /gene="rpsQ"
                     /locus_tag="IVY71_001421"
     CDS             5082..5348
                     /gene="rpsQ"
                     /locus_tag="IVY71_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7985482.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5406..5774
                     /gene="rplN"
                     /locus_tag="IVY71_001422"
     CDS             5406..5774
                     /gene="rplN"
                     /locus_tag="IVY71_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7985483.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5811..6119
                     /gene="rplX"
                     /locus_tag="IVY71_001423"
     CDS             5811..6119
                     /gene="rplX"
                     /locus_tag="IVY71_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7985484.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6147..6686
                     /gene="rplE"
                     /locus_tag="IVY71_001424"
     CDS             6147..6686
                     /gene="rplE"
                     /locus_tag="IVY71_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7985485.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6705..6890
                     /locus_tag="IVY71_001425"
     CDS             6705..6890
                     /locus_tag="IVY71_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7985486.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6926..7324
                     /gene="rpsH"
                     /locus_tag="IVY71_001426"
     CDS             6926..7324
                     /gene="rpsH"
                     /locus_tag="IVY71_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7985487.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7356..7892
                     /gene="rplF"
                     /locus_tag="IVY71_001427"
     CDS             7356..7892
                     /gene="rplF"
                     /locus_tag="IVY71_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7985488.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8053..8409
                     /gene="rplR"
                     /locus_tag="IVY71_001428"
     CDS             8053..8409
                     /gene="rplR"
                     /locus_tag="IVY71_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7985489.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8430..8930
                     /gene="rpsE"
                     /locus_tag="IVY71_001429"
     CDS             8430..8930
                     /gene="rpsE"
                     /locus_tag="IVY71_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7985490.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8945..9124
                     /gene="rpmD"
                     /locus_tag="IVY71_001430"
     CDS             8945..9124
                     /gene="rpmD"
                     /locus_tag="IVY71_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7985491.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9168..9608
                     /gene="rplO"
                     /locus_tag="IVY71_001431"
     CDS             9168..9608
                     /gene="rplO"
                     /locus_tag="IVY71_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7985492.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9608..10903
                     /gene="secY"
                     /locus_tag="IVY71_001432"
     CDS             9608..10903
                     /gene="secY"
                     /locus_tag="IVY71_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7985493.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10965..11612
                     /locus_tag="IVY71_001433"
     CDS             10965..11612
                     /locus_tag="IVY71_001433"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7985494.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11803..12021
                     /gene="infA"
                     /locus_tag="IVY71_001434"
     CDS             11803..12021
                     /gene="infA"
                     /locus_tag="IVY71_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7985495.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12054..12170
                     /gene="rpmJ"
                     /locus_tag="IVY71_001435"
     CDS             12054..12170
                     /gene="rpmJ"
                     /locus_tag="IVY71_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7985496.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12188..12553
                     /gene="rpsM"
                     /locus_tag="IVY71_001436"
     CDS             12188..12553
                     /gene="rpsM"
                     /locus_tag="IVY71_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7985497.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12581..12970
                     /gene="rpsK"
                     /locus_tag="IVY71_001437"
     CDS             12581..12970
                     /gene="rpsK"
                     /locus_tag="IVY71_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7985498.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13050..13988
                     /locus_tag="IVY71_001438"
     CDS             13050..13988
                     /locus_tag="IVY71_001438"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7985499.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14016..14396
                     /gene="rplQ"
                     /locus_tag="IVY71_001439"
     CDS             14016..14396
                     /gene="rplQ"
                     /locus_tag="IVY71_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7985500.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14668..15507
                     /locus_tag="IVY71_001440"
     CDS             14668..15507
                     /locus_tag="IVY71_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7985501.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15483..16352
                     /locus_tag="IVY71_001441"
     CDS             15483..16352
                     /locus_tag="IVY71_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7985502.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16342..17139
                     /locus_tag="IVY71_001442"
     CDS             16342..17139
                     /locus_tag="IVY71_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7985503.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17342..18088
                     /gene="truA"
                     /locus_tag="IVY71_001443"
     CDS             17342..18088
                     /gene="truA"
                     /locus_tag="IVY71_001443"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7985504.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            18753..18990
                     /locus_tag="IVY71_001444"
                     /pseudo
     CDS             18753..18990
                     /locus_tag="IVY71_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19134..19910)
                     /locus_tag="IVY71_001445"
     CDS             complement(19134..19910)
                     /locus_tag="IVY71_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7985505.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT         6663 a         3593 c         4297 g         5479 t
ORIGIN      
        1 aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa
       61 tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata
      121 tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga
      181 tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg
      241 tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga
      301 tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat
      361 cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc
      421 cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt
      481 cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac
      541 caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca
      601 aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga
      661 aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg
      721 tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag
      781 ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc
      841 atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc
      901 taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta
      961 ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac
     1021 aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc
     1081 cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc
     1141 gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga
     1201 tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag
     1261 ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac
     1321 aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta
     1381 atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac
     1441 acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag
     1501 gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag
     1561 tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag
     1621 caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct
     1681 tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg
     1741 actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta
     1801 acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa
     1861 atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat
     1921 gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga
     1981 tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc
     2041 ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa
     2101 attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac
     2161 attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga
     2221 taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat
     2281 ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa
     2341 tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta
     2401 cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa
     2461 gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc
     2521 gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa
     2581 gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca
     2641 ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt
     2701 ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac
     2761 gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca
     2821 atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct
     2881 cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca
     2941 cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt
     3001 caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt
     3061 cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc
     3121 accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt
     3181 gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca
     3241 gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca
     3301 gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact
     3361 tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat
     3421 ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc
     3481 tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc
     3541 aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc
     3601 agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc
     3661 ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc
     3721 tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat
     3781 aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg
     3841 atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc
     3901 gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg
     3961 caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag
     4021 gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc
     4081 atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa
     4141 tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac
     4201 gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag
     4261 cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg
     4321 cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag
     4381 tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat
     4441 aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg
     4501 ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc
     4561 acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa
     4621 acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat
     4681 tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg
     4741 tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg
     4801 tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg
     4861 tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag
     4921 aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag
     4981 aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc
     5041 gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc
     5101 aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg
     5161 ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat
     5221 acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa
     5281 ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta
     5341 ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac
     5401 acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa
     5461 ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga
     5521 tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta
     5581 aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat
     5641 ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct
     5701 ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac
     5761 cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta
     5821 aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac
     5881 tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac
     5941 accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc
     6001 atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag
     6061 aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa
     6121 agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag
     6181 taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg
     6241 aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata
     6301 aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa
     6361 aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc
     6421 gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac
     6481 gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta
     6541 ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta
     6601 tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac
     6661 aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa
     6721 tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac
     6781 gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg
     6841 aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt
     6901 cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac
     6961 tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat
     7021 taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat
     7081 cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg
     7141 tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga
     7201 cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt
     7261 tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg
     7321 gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg
     7381 ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac
     7441 caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg
     7501 aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc
     7561 gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac
     7621 taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt
     7681 actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca
     7741 cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc
     7801 gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg
     7861 tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag
     7921 cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc
     7981 cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa
     8041 gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt
     8101 cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct
     8161 aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc
     8221 tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt
     8281 aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt
     8341 ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta
     8401 gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat
     8461 tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc
     8521 tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg tttggtacag
     8581 gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact
     8641 tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg
     8701 gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag
     8761 ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa
     8821 atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag
     8881 aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac
     8941 aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca
     9001 acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc
     9061 taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat
     9121 ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg
     9181 aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat
     9241 ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg
     9301 gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag
     9361 gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct
     9421 ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg
     9481 aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag
     9541 cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg
     9601 tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat
     9661 cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg
     9721 cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt
     9781 ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat
     9841 tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg
     9901 gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt
     9961 agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt
    10021 aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc
    10081 cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg
    10141 cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat
    10201 ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt
    10261 ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc
    10321 tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta
    10381 tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat
    10441 tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta
    10501 caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat
    10561 tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc
    10621 ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga
    10681 agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt
    10741 agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt
    10801 aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga
    10861 aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt
    10921 tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa
    10981 tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta
    11041 ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg
    11101 gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg
    11161 gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct
    11221 ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa
    11281 aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg
    11341 gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag
    11401 ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac
    11461 ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt
    11521 attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat
    11581 ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta
    11641 tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga
    11701 gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt
    11761 tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg
    11821 attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta
    11881 gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt
    11941 atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt
    12001 attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag
    12061 taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg
    12121 ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc
    12181 tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc
    12241 acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg
    12301 tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc
    12361 agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa
    12421 acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg
    12481 tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg
    12541 caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa
    12601 tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt
    12661 caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc
    12721 tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc
    12781 agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa
    12841 aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt
    12901 gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg
    12961 tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac
    13021 ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa
    13081 aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg
    13141 ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca
    13201 tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag
    13261 aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag
    13321 aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta
    13381 tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag
    13441 gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg
    13501 aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg
    13561 ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata
    13621 aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt
    13681 ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa
    13741 atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga
    13801 tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata
    13861 ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac
    13921 gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag
    13981 aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca
    14041 catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac
    14101 gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca
    14161 ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg
    14221 aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac
    14281 tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga
    14341 agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc
    14401 acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt
    14461 tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca
    14521 tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata
    14581 aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag
    14641 gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat
    14701 tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat
    14761 aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa
    14821 acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg
    14881 gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca aaatccagac
    14941 aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg
    15001 attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct
    15061 gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt
    15121 gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta
    15181 gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa
    15241 ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta
    15301 gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt
    15361 cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg
    15421 aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg
    15481 ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac
    15541 aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag
    15601 ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa
    15661 caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt
    15721 taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg
    15781 aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag
    15841 aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg
    15901 aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc
    15961 ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg
    16021 gtagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg
    16081 tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg
    16141 aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc
    16201 taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta
    16261 aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc
    16321 attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga
    16381 gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc
    16441 ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta
    16501 ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg
    16561 atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact
    16621 tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt
    16681 ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct
    16741 ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca
    16801 gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac
    16861 gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac
    16921 ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc
    16981 aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga
    17041 agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt
    17101 gtattattaa ctgtgctact gatctttttg agaggataaa cgttcagtgg gccgctatct
    17161 attcttttct tgctgatctg tttgcaagaa aggatgcgag cagtgtatga caagaaatat
    17221 tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga
    17281 aaaaatttta taaaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa
    17341 catggtacgt tacaaagcaa tcattgcata cgacggaaca aattttaatg gattccaaaa
    17401 acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa
    17461 tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac atgcgattgg
    17521 gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct
    17581 agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt
    17641 ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc
    17701 acgcagtcct tttcgacgcc attatgcaag ttattttcct taccctcttg atttggaaaa
    17761 aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc
    17821 aggaagctcg atagaggata aagtacgtac gatctacgag gctaagatgg aggtaaatga
    17881 aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg
    17941 gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc
    18001 agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacatc ctgaaggact
    18061 ttatttgtat gaagtgaaat atgaataaga ggttgtgaca gaagtggtca gcttcaagaa
    18121 ataagaagga atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg
    18181 aaggagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag
    18241 taataagaac aaccaaacaa aaatagctcg tcatattttt cgtttggttg ggcttattac
    18301 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cgttttgacc
    18361 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    18421 taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    18481 tctgagtcac aagtaacaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact
    18541 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    18601 aaacgatgga acgttgtttt gattgccctt aatgaggaca gtcggaacag gtttgtcatt
    18661 tttatattta aatggcacaa atttttctaa ttatttgtca tttagcgctg aaaatttttg
    18721 ctatttatct aaaaaagtga taactactta agatgaagca gaatatttac gccatacggc
    18781 atacaaccgc gaaaaatata aaaaaaagaa aacgattgaa cgcgtttttg cagtcgcaaa
    18841 ggaaaagcat ggaatgcgct acatcaagta tcgagacttg gaaaacaatc aggtgtatac
    18901 gctgattgtt tttgctgcaa tgaatctgaa aaagttgtcc acttggcagt tggaacatat
    18961 gtcttcatat cttttattat acaaaaatag caaaagcacc agtctacact cttagtaaag
    19021 aggaaactga tccttttgtc tacattctta aagtacttaa actaagtact ttattttact
    19081 ctttaggatt gatacagtag catctatccc tttattaata atcttttatt tttttaaata
    19141 aagatgttgt ctccatcttc aggcactaaa actttttcgg aaattccttt ttctttattg
    19201 aaagacttta attcttctct tgacagtccc caatggttta cagcttccat atgaacggca
    19261 ataattgtgg aatctggtat tgctttatgc aattcatata aatcgtcttt ccccataata
    19321 agagattctc caactaaaaa atgattatct ccagcatttg ctactatgat ttctggatta
    19381 tactgatcaa tagtttgttt aacagcttca taccaaactg tatctcctgt aaagtatagt
    19441 gtcttctcag tcgaatgttt gaaaacaata ccgcaaacat tacctacata attcaagatt
    19501 tctcctctgc catgtttgcc ttttgtttta accaattgta tagattcaaa aactgtgtct
    19561 tctcgtagta cctccacatt tttaaaacca tatagtcgaa tttcattcgc atcattttca
    19621 tcttgcacaa ataattttat ttctttaggt aggacctctt tcgctatatc gtcccaatga
    19681 tctagatgta aatgtgttac aataacagca tcaataccgt tgataatatc ttcgactggc
    19741 attggcaaat caactagagg attcatttga tcttttcttg gtgatggaaa aggacctttc
    19801 gaaataaatg gtggaagtgt tcctttttcc gctaacattg gatcaattaa aaatttttta
    19861 cctccatagt ctaagatagc tgttgcatta cgaatgtgat gtatattcat aaacgttgtc
    19921 tcctttttgt tgatatgaaa tatttttagt ataattcaat tccatacaat aaataaaccg
    19981 aacaaaaaaa gtcttttact atttttactt aatttatgaa aggttgttta cc
//
LOCUS       DACCXW010000049        20032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCXW010000049 DACCXW010000000
VERSION     DACCXW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.049"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            65..877
                     /locus_tag="IVY71_001446"
     CDS             65..877
                     /locus_tag="IVY71_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7985506.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            874..1674
                     /locus_tag="IVY71_001447"
     CDS             874..1674
                     /locus_tag="IVY71_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7985507.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1835..3103
                     /gene="rseP"
                     /locus_tag="IVY71_001448"
     CDS             1835..3103
                     /gene="rseP"
                     /locus_tag="IVY71_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7985508.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3171..4880
                     /locus_tag="IVY71_001449"
     CDS             3171..4880
                     /locus_tag="IVY71_001449"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7985509.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5092..9450
                     /locus_tag="IVY71_001450"
     CDS             5092..9450
                     /locus_tag="IVY71_001450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7985510.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9595..10068
                     /gene="rimP"
                     /locus_tag="IVY71_001451"
     CDS             9595..10068
                     /gene="rimP"
                     /locus_tag="IVY71_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7985511.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10091..11266
                     /gene="nusA"
                     /locus_tag="IVY71_001452"
     CDS             10091..11266
                     /gene="nusA"
                     /locus_tag="IVY71_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7985512.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11288..11581
                     /locus_tag="IVY71_001453"
     CDS             11288..11581
                     /locus_tag="IVY71_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7985513.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11578..11889
                     /locus_tag="IVY71_001454"
     CDS             11578..11889
                     /locus_tag="IVY71_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7985514.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11902..14208
                     /gene="infB"
                     /locus_tag="IVY71_001455"
     CDS             11902..14208
                     /gene="infB"
                     /locus_tag="IVY71_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7985515.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14235..14582
                     /gene="rbfA"
                     /locus_tag="IVY71_001456"
     CDS             14235..14582
                     /gene="rbfA"
                     /locus_tag="IVY71_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7985516.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14739..14912)
                     /locus_tag="IVY71_001457"
     CDS             complement(14739..14912)
                     /locus_tag="IVY71_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7985517.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15360..17722
                     /locus_tag="IVY71_001458"
                     /pseudo
     CDS             15360..17722
                     /locus_tag="IVY71_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18090..19013
                     /gene="truB"
                     /locus_tag="IVY71_001459"
     CDS             18090..19013
                     /gene="truB"
                     /locus_tag="IVY71_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7985518.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19017..19958
                     /gene="ribF"
                     /locus_tag="IVY71_001460"
     CDS             19017..19958
                     /gene="ribF"
                     /locus_tag="IVY71_001460"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7985519.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT         6868 a         3522 c         4396 g         5246 t
ORIGIN      
        1 aacagttaga tattgctata atacttaaga tagtgggaat cttttaagta taggaggaaa
       61 aagaatgtta cgtttttttc cgcaaaaaaa caaatatatt caagaaaaaa gcgaatttca
      121 atttaatacc gatggaccaa ttcctcagca cattgccatc atcatggacg gcaatggtcg
      181 ttgggcgcaa aaccgtcgtt tgccacgagt tgctggccat aaagaaggca tggaaacagt
      241 aaagaaagtc acaaaaaaag catcaaggct tggagtgaaa gtgttgacgc tttatgcctt
      301 ttcgactgaa aactggaagc gaccaaaaga tgaagtaagt tttctcatgc aactacctgt
      361 cgacttcttt gatacgttcg taccagaatt gattaaagaa aatgtcaaag ttcatgtcat
      421 gggttatgaa aacgtattgc cagaacatac gcaagatgct gtaagacgtg caattgagca
      481 aacaaaaaac aatactggaa tggtattgaa ttttgctttg aattatggaa gcagagcaga
      541 gatcgtgact gctgtccaag aaattgccga agaagtagca aaaggagaaa tacacgcaga
      601 agaaatcgat gatgaattga ttgctaagca tttaatgaca ggtttcttgc ctaaagagtt
      661 acaagatcct gagctgatga tccgtacaag tggagaagag cgaattagta attttctgtt
      721 atggcagatc gcatatagtg agctgtattt tacaaaagcg ctttggcctg attttgatgg
      781 tgcgcatctt gaagaagcaa tcgcctctta tcaaaatcga gaccgtcgat tcggcggtgt
      841 aaaaaaaaca gaagatgaga aaggggatca gtcatgaaac aacgtgtggt cacagcagcc
      901 gtggcgctag tcttgtttat tccgattata tggatcggag gcatagcggt tgagcttaca
      961 gcagcaatct tagcagtaat aggagtttac gagctgtttc ggatgaaagg attgacactc
     1021 ttaagttttg aaggagtttt atccgccgtt ggtgcagtgt tacttgtttt gccaaaagaa
     1081 agatggtttt tctttcttcc agagaatgta agtacattta tgttatttta tcttatagtt
     1141 atgattcttt taggaacatc agttatttct aaaaatacgt atacgattga tgaagcaggt
     1201 tttcctgtgc ttgtcagctt gtatgtggga ataggtttcc agaattttgt caatgcacga
     1261 acagaaagct tggctgtttt gatatttggt ctatttattg tttgggcaac tgatatcgga
     1321 gcatatatgg ttggtcgacg ttatggtcag cataagttaa gtcctgatat ttcgcccaac
     1381 aaaacgattg aaggtgcgct cggtggtatt ggtagtgctg ttgtagtcgc atttctttat
     1441 ttattgctat acccagcaaa agaactattt aatcatggta tgtttgtcat gcttctgatg
     1501 acgattattt tttcagcagt cggacaattt ggtgatttgg tcgaatctgc aatcaaacgt
     1561 cattatgatg tgaaagactc gggaaatatc ttaccaggcc atggtggaat cttagaccgt
     1621 ttcgacagtt tgctgtttgt atttcctatc atgcatttat ttggcattat ttagtaagta
     1681 tacctgcgtt tctttgtgaa tttcataaaa gacgcaggtt tttatttttc attatattga
     1741 tactattggt gtctttggta tactatggta aaattatagt gttttatcgt tgttttataa
     1801 aaaacacaaa aaacaagtaa aggagaaaaa agctatgaaa acgattctga catttatcat
     1861 cgtttttggt atattagtga ttgttcatga gtttggtcat ttcttctttg cgaaacgatc
     1921 aggaatcctc gttcgcgaat ttgctatcgg tatgggtccg aaaatttatg ggcatcaagc
     1981 aaaagacggg acaacatata cgcttcgcct attacctatt ggcggttatg ttcgaatggc
     2041 aggcaatggg gatgatgaaa cagaaatggc accaggaatg cctctttctc ttttactaaa
     2101 ttctgacggc atcgtagaga agataaattt aagtaagaaa atccaattga caaatgctat
     2161 cccgatggaa ttgagccgtt atgatttaga agatgagctg actatcactg gttatgtgaa
     2221 tggagacgaa acagaagtag taacctaccc tgttgatcac gatgcgacga ttattgaaaa
     2281 cgacggaaca gaaatcagaa ttgcgccaaa agacgtacag ttccaatcgg ctaaattgtg
     2341 gcagcgtatg ctgaccaact ttgctggacc catgaataac tttattttgg caatcctgtt
     2401 attcatcatt ctggcattta tgcaaggtgg tgttcaagtg acgaacacta atcgcgtagg
     2461 agagatcatg cctaatggag ctgcggcaga agcaggctta aaagaaaatg atgaggtagt
     2521 cagtgtggac ggaaaagaaa tccattcatg gaatgatctg acaacagtca ttacgaaaaa
     2581 tccggataaa acattagact ttaagatcga acgagaaggt caggtgcaat ctgtcgacgt
     2641 gacaccgaaa tctgtagagt caaatggcga aaaagttggc caacttggta taaaagcccc
     2701 aatgaataca ggttttatgg ataagatcat tggagggacg cgtcaagctt tcagcggttc
     2761 attggaaatt ttcaaagcct taggatcact atttacaggt ttcagtttag ataaattagg
     2821 cggacctgtc atgatgtacc aattgtcttc agaagcagca aatcaaggtg taacaactgt
     2881 tattagtttg atggcgcttc tttcaatgaa tctcggaatc gtcaatctgc ttccgattcc
     2941 tgccttagat ggcgggaaat tagtcttgaa tatttttgaa gggattcgcg ggaaaccatt
     3001 aagtcaagaa aaagaaggaa tattgacatt agctggcttt ggatttttaa tgttgttgat
     3061 ggtgctagtt acatggaacg atattcaacg attctttttc tagaaaaagt tgacaaatag
     3121 ggtataataa caaacgataa ctattttgag gaaataaagg agtatataga atgagacaat
     3181 caaaaatgtt gattccaaca ttacgcgaag ttccaaatga tgcagaagta ttaagccatc
     3241 aaatcttgct aagagctgga tatatccgcc aagtatcagc gggtatttat tcatatctgc
     3301 cattagcaaa tcgtgtattg gaaaaactga aacgcattat gcgggaagaa tttgaaaaaa
     3361 tcggagcggt agaaatgctg atgcctgcca tcttgccagc agaactttgg gaagaatccg
     3421 ggcgttacga aacatatgga ccaaacttgt atcgtttcaa agaccgcaat gatcgaaaaa
     3481 tgatccttgg accaactcac gaagaaactt ttactgattt gatgcgtaat gaaatcaatt
     3541 catataagaa attaccttta aatctttatc aaatccaacc taaatatcgt gacgaaaaac
     3601 gacctcgctt cggtttgctt cgcggaagag agtttatcat gaaagatgcg tattctttcc
     3661 atgcaacgga agaaagtttg gacgagacat ataaagatta cgaaaaagcc tacaccgaag
     3721 tattcaagcg ttgtggctta gaatttcgct cgattatcgg agacggtggc gcaatgggcg
     3781 ggaaagactc aaaagaattc atggcgatct cagaaatcgg ggaggatacg atctgttatt
     3841 cgactgaaag tgattatgct gcaaacttgg agatggctac aagctactat gtatcaaaaa
     3901 aatcgcatga aacacagctt gaattggaga aagtagctac acctaacgta aaatcaattg
     3961 acgaagtagc agatttcttt tctgttgaac ctcaaaaaat catcaagtct gtattgttca
     4021 ttgcggatga acaacctgtc cttgttttgg tacgcggaga ccatgaagtc aatgacgtaa
     4081 aactgaaaaa ctttttatct gctgactttt tagaagaagc aacagaagaa gaagcacaaa
     4141 aatatctagg tgctgagttt ggttctgtcg gaccagtcgg tgtttcagat gacgtaaaag
     4201 tatatgcaga ccgttatgtc caagatttag cgaatgccat tactggagcg aacgaaacag
     4261 gattccacta taaaaatgtg aatccagaac gcgatttcac tgtactttct tatgaagatc
     4321 ttcgttttgt acaagaagga gatccttcac cagacaacaa tggtgttctt gcctttactc
     4381 gaggaatcga aatcggacat atcttcaaaa tcggcactcg ttacagtgaa tctatgggag
     4441 caactgtact agacgaaaat ggacgagaaa aatttgtgat tatgggctgt tatggtatcg
     4501 gtgtaagtcg tttattatca gcaatcgtag aacaacattc agatgaacaa ggaatcaact
     4561 ggccaaaagg aattgcgcca tttgatttgc atatcgtcca aatgaatcta aaagacgaat
     4621 accaaacaaa cctgacgaat gaactagaag aagcgatgac aaaagctggc tatgaagtac
     4681 tggttgatga cagaaatgaa cgcgcaggag tgaaattcgc ggattctgat ttgattggct
     4741 gtccaatcag aatcactgtc ggtaaaaaag cagtggatgg aatcgtagaa gtgaaaatca
     4801 agaaaactgg cgaaatggtt gaagtacgta aagaagaatt ggtgaatact ttaccaatcc
     4861 tcttgaacca aggcgaataa taagcgttaa aacacagaaa ataaaaatag ctgaatcagc
     4921 tgtttttgac aatctgatga ttcgctttta aaattggaac tgcgatttga atcgcagttc
     4981 caatttttta atgtaaaact cagaaaaaac gagtatacta aaaggtactg aaaaagaaga
     5041 ggataagatg cgatcaattg cattgtttct catcagaaag ggcgtatatc tttgtcagaa
     5101 aaacgagatt tatttgaaaa actgttggaa caaatccaac tagaggaagc agaaaaaaat
     5161 catccactgc ttactgctgg tgaaatggat agtgtagtgg tccatcgtaa atcgcgttta
     5221 tgggaattta cgctgcattt ttccaaaatt ttgcctatta tgctatatcg gtcattgact
     5281 cagcatctga ctatcgcttt taaagatatt gcacaagtca aactgaatat cttagcagat
     5341 gaccagacat tcgatgaaca gctcttgcaa gattactggg cccaagcttt ggaaaatcag
     5401 caatgcgata caccactagc acagcaagta ttgaaaacgc aggtacctgt gataaaagat
     5461 cgtaaagtca tcttgccaat cgacagtaca ggagctattt cttatttgaa gcaacaatac
     5521 ttgccattag tagaagagtt gttcgtcagt tacggattcc caaaatttcg tatcgaacca
     5581 gaagtggatg agcagcaagc ggaacgagta ttgaaacttt ttgaagaacg caaacaagaa
     5641 caggcagaag cctttatgaa gcaagcagct gaaagtctag ttgtccacga acaaaagaaa
     5701 aaggaaagaa aagagcaatc gccagctctt gaaggaccca tccacatcca gctaggacga
     5761 aatattccag ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga
     5821 gtcacacttg aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag
     5881 atcttgactt tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac
     5941 ggcgaaaaag atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga
     6001 ggaagtattc aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata
     6061 gaagtcaaac atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt
     6121 catgttcata gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca
     6181 caagcaggga aatggggaca ccgagctatc gccattacag accacggtgg tgcgcaagcc
     6241 tttccggaag cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa
     6301 gcgaatgtcg tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt
     6361 gaagccacat atgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg
     6421 atcatcgagc tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa
     6481 tttatcgatc caggacatcc tttgtcacga acaacggtag atctgactgg tatcacggat
     6541 ggaatggttc gaggatcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca
     6601 aaagatacaa ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc
     6661 tacgcaaggt atggtattcc tgaagcggcc aatccagtca ttgatacgtt ggaattagct
     6721 agatatctgt atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaaatttggt
     6781 gtcagcttgg aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca
     6841 tggatctttg tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat
     6901 gagcatatcg gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta
     6961 gcaaaaaatc aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa
     7021 tacttcgaac gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta
     7081 ttgatcggct cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta
     7141 gaagaggcaa gaaaccgagc aaaattctac gattatattg aagttatgcc aaaagcggtc
     7201 tatgcgcctt tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc
     7261 caaaatttgg tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta
     7321 cattatctga acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga
     7381 gcgaatccgt tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg
     7441 ctaactgcat ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg
     7501 aataaaatcg cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg
     7561 aagattccag gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg
     7621 tacggagatc cgttacctga gatcattcaa aaacgattaa aaaaagagtt gaattctatt
     7681 aatggtaatg gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat
     7741 gaagatggct atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg
     7801 acaggaatca ctgaagttaa tccgttagcc cctcattatt attgtccaga gtgccaatat
     7861 tccgagtttt ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt
     7921 ccaaaatgtg gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta
     7981 ggattccatg gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca
     8041 gaagcccata actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg
     8101 attggtacag tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat
     8161 aatctgcagt ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa
     8221 cggacaaccg gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat
     8281 gattttacgc cgatccaata tccagcagac gatcaaaatt cggaatggaa aacgactcat
     8341 tttgattttc actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac
     8401 ccaactgtta ttcggatgct tcaagattta tcagggatcg atccacaaac gatccctacg
     8461 gatgacccgg aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa
     8521 caaatttatt ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga
     8581 gggatgctag aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta
     8641 tctcatggta cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg
     8701 acactggcgg aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga
     8761 ttagatagcg ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag
     8821 atccctgatg aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac
     8881 tggtatatcg attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct
     8941 tacgtattga tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac
     9001 tgtgcatact tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa
     9061 gatgctgtga aacaagcaat gaaagaaatc acggacaaag gattagatgc ttccgtaaaa
     9121 gaaaagaatc aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa
     9181 tttggaatga ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg
     9241 ttgatcgcac catttagagc tgtcccaagt ttgggaacca acgtggcaaa acaaatcgtt
     9301 gaagcaagaa aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt
     9361 tcaaaaacat tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa
     9421 aatcaattat cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt
     9481 aagcagagta tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt
     9541 cgctcactct ttttaatgga attacgcact taatttttta aggaggcgaa acttttgagt
     9601 agcgtcgttg aaacagtcac agaaatggtg acaccgattt tagatgaaca aaattttgaa
     9661 cttgtcgaag tcgaatttgt aaaagaagga aaaagctggt tcttacgagt atttattgac
     9721 aaagaaggcg gtatcgatat cgaagaatgc gcctttgtca gtgagaaact gagtgaaaaa
     9781 cttgatgcag cagatccaga tccgattcct caagcttatt tcctggaggt atcctccccg
     9841 ggagcagagc ggccattgaa aaaagaaagc gactatgaaa aggcacttgg tgattatgtc
     9901 catgtctcct tataccaacc agtagatggc gaaaaacaat atgaaggatt tcttcaatca
     9961 tttgatgctg aacagcttac attgaaaata cgcataaaaa cacgagaaaa agaattgact
    10021 tttgaccgaa aaaatattgc aaaagctcgc ttagctatcc aattttaatc acggagagga
    10081 atcagaaaaa atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg
    10141 gatctcaaaa gaaatcgtga tcgatgcttt agaagcagca ttggtttctg catacaaacg
    10201 tcactatggc caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca
    10261 tgtctatgcg gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt
    10321 gaaagatgca cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt
    10381 aacaccaaaa gattttgggc ggatcgctgc gcaaacggct aaacaagtta ttttacagcg
    10441 tgtacgtgaa gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat
    10501 catgcaagga atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat
    10561 tgaggcagtt ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg
    10621 aatcaaagtt tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag
    10681 ccgcagccat ccagacctat tgcgccgctt atttgagcaa gaagtaccag aagtctatga
    10741 tggaatcgtt gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt
    10801 tcgctcaaat gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg
    10861 agttcaagca atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga
    10921 agatccagct gtattcattg caaatgcatt gaatcctgca caagtcatgg atgtgatctt
    10981 cgatgagaca aatccaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc
    11041 aatcggtaaa cgcggacaaa acgcacgttt agctgctaaa ttgactaacc ataaaatcga
    11101 tatcaaatca gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac
    11161 agaagaactg catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac
    11221 aattgcattc aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg
    11281 caaagagatg aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa
    11341 acctaaaaaa gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc
    11401 aactggaaaa atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca
    11461 agcttgggat aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta
    11521 tcaagaactt cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg
    11581 aatacagtta atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa
    11641 ctaatcacgg gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg
    11701 tttgtggcaa ccgatgcaag cgagaataca agaaaaaaga taaaagataa gagttcatat
    11761 tatgaagttc cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa
    11821 cctcgcaagg tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt
    11881 gaaggttagg aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc
    11941 aaaaaatcta gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac
    12001 catatgggtg cgattactag caatgatgag aagaaattgc aacaagcgtt caaaaaccca
    12061 agcacgaaac aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac
    12121 gatcaacgta aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat
    12181 caagatcgcg gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa
    12241 aataatacgg gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa
    12301 aaccgtacta acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat
    12361 aaccgtaatc gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca
    12421 gctgtaccac cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg
    12481 aacgtagcag atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta
    12541 tttatgcttg gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg
    12601 ttagcgacgg actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc
    12661 gataaattct tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagtc
    12721 gtaacgatta tgggacacgt agaccatggg aaaacaactt tattggatac attacgccat
    12781 tcacgtgtaa caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc
    12841 gatattgatg gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt
    12901 atgcgtgcac gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat
    12961 ggcgtcatgc cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc
    13021 atcgtcgctg taaataaaat tgataaacca ggtgcaaacc ctcaacatgt tatgcaagaa
    13081 ttaagtgagt atgagttaat tcctgaagca tggggtggag aaacgatctt tgttgagatc
    13141 tcagcgaaat tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa
    13201 gtagaagatt taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt
    13261 cttgataaag gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcatgtt
    13321 ggtgacccaa tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt
    13381 ggccgtcgcg ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat
    13441 gtacctcaag caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg
    13501 ggtgaagaac gcgcgaaacg tgcattatta gaaaaccgtg ctgccacaag ccgtgtgact
    13561 ttggataact tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc
    13621 aaagcagatg tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg
    13681 gaaggtgtcc gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta
    13741 accttagcag cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag
    13801 gcaaaagtgc aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa
    13861 gccatcgaag aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa
    13921 atcaccggtc aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca
    13981 ggtgcctatg taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac
    14041 ggaatcgtaa tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa
    14101 gaagtcaaaa tgggttatga atgtggtgca atgattgaaa aattcaatga catcaaagta
    14161 gatgacgtaa ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa
    14221 ggaggccaat cattatggct aactaccgtg accgccgagt cggtcaagaa atcttaaaag
    14281 aagtcaatga tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca
    14341 cagatgtgaa agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg
    14401 acttagcatc agataagaaa acagcacaag atggattgaa taaagcatca ggattgattc
    14461 gtcgagagtt agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg
    14521 atgaatcagt tgtctatggc aaccatatcg atgaattgct tcgcgacttg aacaaagact
    14581 aatcaaaaac tcccgcaaat cctgtgaaat caggggttgc gggatttttt tgttttcatt
    14641 aagacgtatt acctaagagt tccatgactt tagtcttgcg gcgtgtcagc taaccaaaaa
    14701 agagaaaaag aaaagtaaaa aattcgactt tcttttttct agtagtatat aaatttttgc
    14761 atatagataa agatcaagat accgataacg gaagctagat tgtaatctat tttagtgaca
    14821 gtcaggtata gccaagtaac tagaagatca gtggaacctg caccataaaa gtttgagttt
    14881 gtcggtccgt tgacagttaa aagaaaaatc acgttgaagt tattgatatt tccgattacg
    14941 ccgcttcgat ttggtcacga tccaaatttt ggataatccc tgtagaaatt agcatagtag
    15001 ctgggatacc aattcacata ttgacaaaga taacagagac ttttacataa aacggatcaa
    15061 tcaggaaagg aattgctcga tccagcccca gttttccagc aatgtgttaa ctggcccagc
    15121 gtcattcagc aaattatgca tgatgaggag cgaaatgaac tgcggaacag ccattgtgat
    15181 aacgacaagt tttttggatc aatgtaaaaa acgtcagcga aaggaaaaat ttcgtggttt
    15241 tttatacctg ttcatttggc tagatataaa atccaaacaa atgaacagaa gtgcttatta
    15301 ggtttcaatt gggagtatct gcgctagaat aagagtaata atgaaaagga gtgttatgta
    15361 tggattattc tagtaaagaa tactttgata aaatgacggc ttggtggcgt gctgctaatt
    15421 atctttctgt tgggcaactt tatcttaaag ataacccctt actgagacgt acactgaaac
    15481 ccgaagatgt caaaaaacat ccaatcggtc attggggaac gattcctggc caaaatttta
    15541 tttatgtcca tttgaaccga gtcatcaata aatatgattt gaacatgttt tatattgaag
    15601 ggccaggaca cggcggacaa gtcatggtat cgaatgctta tttagatgga agttacacag
    15661 agatctatcc agaagtaacg gaagatgaga caggaatgca aaaattattc aaacgatttt
    15721 catttcctgg aggcattgct tctcatgcag caccagaaac accaggttct atccatgaag
    15781 gcggagagct tggatactct ctttcacatg cagtcggagc tgtattagac aatcctgaag
    15841 taatctctgc tgtcgtgatt ggtgatggtg aagcagaaac cggtccttta gctggaagct
    15901 ggttctcaaa tgtctttatc aatccagtga tagatggagc tgttttaccg atccttcatt
    15961 taaatggtgc aaaaatagcc aacccaacga tcttggcacg taaatcagat ggagaattag
    16021 caaattactt taacggactt ggctgggaac ctttctttat tgaaggaaat gattcagaaa
    16081 aattaaatcc tgttatggct gaaaaaatgg atcaagcaat cgagaaaatt aaatccatcc
    16141 aaaaagaagc tcgcctgaag acagcaacag atgtcgtgat gccaaaatgg ccagtattga
    16201 tcgtacgcac accaaaagga tggaccggtc caaaagaatg ggacggggaa ccaattgaag
    16261 gaaccttccg agcacaccaa gtaccgattc ctgtagatca agaacatatg gatcatgcag
    16321 atgctttact ccgttggctg aaatcctatg aaccagaaaa attatttgat gcacaaggac
    16381 gtattttaga agaaatcaga gaaatcgcgc ctactggtga ccaaagaatg gcaaaaaatc
    16441 ctatcaccaa tggtggaatc gatccaagac cgcttattat gccagattgg aaaaaatata
    16501 ctttgcaatt tgaaaaacca ggctccgtta aagcagaaga tatgaccgaa cttggtaaat
    16561 tcgttcgcga gatcattgag aaaaatccag aaaatttccg tatatttgga ccagatgaaa
    16621 caaaatcaaa ccgattgaat caagttttca aaacgacaaa tcgccaatgg atggaaaaaa
    16681 tcgaaccaga aaatgatgaa tggctttcgc catctggtcg cgtcatcgac agtcaattat
    16741 cggaacacca agatgaaggg ttcttagaag ggtacgttct gactggacgg catggttttt
    16801 tgcaagttac gaatcattct tgcgagtggt tgactctatg ttgactcagc acttcaaatg
    16861 gatgcggaaa tcacatgacc tttcttggcg aaatgattat ccttcgctaa atctcattgc
    16921 aagctcaaca gtcttccagc aagatcacaa tggatatagc caccaagatc caggaatatt
    16981 gacacatttg gcagaaaaga aggcggagtt catccgtgaa tatctgcctg cggatgcaaa
    17041 tacattgctc gcagtcatgg ataaagcatt ccgctcgtca gaaaaaatca atttgatcat
    17101 ctcttccaaa catccgcgtg ctcaatttta ctcagcagaa gaagcagccg tcttagtgaa
    17161 tgaaggcttg aaaatcattg attgggcttc gacagcaaaa gaggaggaac ctgaattggt
    17221 cattgctgct gcaggaacgg aatctaactt agaagcatta gcagctgtaa ctttgctgct
    17281 ggaagaattc ccaaaactaa aaattcggtt catcaatgtc gtagatttat tgaaattacg
    17341 tcatccaagt caagatccgc gtgggttgag cgatgaagaa tttgatcaat attttacaaa
    17401 agacaaaccg atcttatttg ctttccacgg ttatgaaaca ttggtccgca cgattttctt
    17461 tgatcgtcat aaccatcatt taatgattca tggctacaaa gaaaatggag atatcacgac
    17521 accatttgac atgcgtgtag taaatgaact tgatcgttat catttggcaa aagacgcagc
    17581 tttaaaaatc aaaggaagtc aagcagaaga ttttgcgaaa aaaatggatc agaaattaca
    17641 agaacatcaa aactatattc gtgaaaatgg tattgatctg ccagaagtat tggattggaa
    17701 atggaaaaat cttgaccaat aaaatgataa aatcgatccc gcaaaataga attttttaca
    17761 ttgattaaga aactgaaaaa ataaataaaa aaaacggaaa aaagactgga agaatggcca
    17821 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
    17881 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaagcat
    17941 aacatgattt ctatttacgt gagcatgaat tttgttacgt ttttattgcc gaaaaataaa
    18001 aaccgttcat tatgatgtca aattcgtcag taattgatgc aatgtgttat aataattcag
    18061 ttaaatcaaa aatgaaatgg agaaaagaca tggacgggtt attaccttta tggaaagaac
    18121 gcggaatgac aagtcatgat tgcgtattca aattaagaaa aatcttacat acgaaaaaaa
    18181 tcggacatgg gggtacgctt gacccagacg tagacggtgt gttgcccatt tgtattggaa
    18241 aagccacaaa agttatcgaa tatttgacag attctgggaa aatctatgta ggcgagataa
    18301 ctctaggttt cagtacaacg acggaagatc gatccggaga acgggtagaa acaaaaccag
    18361 tagatatgtc tttatctgaa aaaaaaatag atgaagtgat ggcttcattt attggtgaga
    18421 tcacccagat accaccaatg tactcagcgg taaaagtcaa cggtaagcgg ttatatgaat
    18481 atgcccgcaa taatgaaaca gtggagcgtc ctgttagaaa ggctcagatt taccgtttta
    18541 tccggacaag tgaattgaca agagacgaag tgacaggaac catttcttgg cgcttcgaag
    18601 tagaatgcgg gaaaggaacc tatgtccgca cattagctgt agatacaggc aagaaacttg
    18661 gttaccctgc gcacatgtct gatctgaccc gaacagcaag tgccggtatg tcccagaagg
    18721 atgctctgac attagaagaa gtagctcaag ctgtgaaaaa tgggacaatc gatgattgtt
    18781 tgctgccaat agaaaaagga gtggaacggt tcaaacgagt agatattact gatgaagtat
    18841 ggcagaaagt aaaaaacggt atgagacttg attatactgt atttgcgctc ccagaaatgc
    18901 cgtcagaaga aattgctttg ttttataaag gaaaagttgt cagtatttat cagccaaacc
    18961 cgaaagaaaa aaacaaatta aaacctagta aagtattgag aaatgaggaa taacgaatga
    19021 atatcatcaa gatcagacat ccttaccaat cagaacaaat tccagaagac gatgtcgttt
    19081 tagtgctggg atttttcgac ggcgtgcata tcggacacca gaaagtcata aagacaggta
    19141 tggaaattgc acaaaaagaa ggattgaaat tagcattgat gactttcaat cagcatccat
    19201 ctattgtctt taaaaagatc gatcctagca gcgtgaaata tttgactagc cttaagcaaa
    19261 aagaagaaaa aatggccgct ttgggtatag attatttata tgaagttgat tttacttctt
    19321 cttttgctca tttagcaccg caggaatttg ttgatcaata catcgtaggg ttacatgcaa
    19381 aatatgctgt ttccggtttt gattatacgt atggaccaaa agacattgca gatgtcgctc
    19441 attttcctga atatgccaaa ggaagattca aaatcgtcac agtttctaaa gaagaagaac
    19501 aaggtgaaaa aatcagctct acccgtattc gtggattact agacagcgga caagtaaaag
    19561 aagcagcaaa actattaggt tgcacatatg aggtagaagg ggtagttgtc catggcgatg
    19621 cacgaggcag actactagga ttccctacag caaatgtcaa agtaaagagc acggttcatt
    19681 tgcctaaaga aggtgtttat gtgtcggaaa taaaaattgg ggaaacatgg tatccagcca
    19741 tgggttcaat tggacataat gatacattcg gtgaaggacg ggagctgact gtagagttgt
    19801 atattttgga ctttaatcaa gatatctatg gtgaacatgt cgcggtacgc tggaatcaat
    19861 ttttaagaga tcaagtgaaa ttttctagtg tagaggaatt gattgaacaa ctcaaggagg
    19921 atgaacaagc aacagctgaa tatttttctc agcaataaaa cattatgcat aaaatctctt
    19981 gtttatgtat aactagcaat tacaataaaa tgggtgataa ataagcccag ga
//
LOCUS       DACCXW010000050        19649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCXW010000050 DACCXW010000000
VERSION     DACCXW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.050"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            22..669
                     /locus_tag="IVY71_001461"
     CDS             22..669
                     /locus_tag="IVY71_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7985520.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            753..1697
                     /locus_tag="IVY71_001462"
     CDS             753..1697
                     /locus_tag="IVY71_001462"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7985521.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1832..3283
                     /locus_tag="IVY71_001463"
     CDS             1832..3283
                     /locus_tag="IVY71_001463"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7985522.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3304..4140
                     /locus_tag="IVY71_001464"
     CDS             3304..4140
                     /locus_tag="IVY71_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7985523.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4140..4799
                     /locus_tag="IVY71_001465"
     CDS             4140..4799
                     /locus_tag="IVY71_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7985524.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4925..5614
                     /locus_tag="IVY71_001466"
     CDS             4925..5614
                     /locus_tag="IVY71_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985525.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5620..6576
                     /locus_tag="IVY71_001467"
     CDS             5620..6576
                     /locus_tag="IVY71_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7985526.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6620..7132
                     /locus_tag="IVY71_001468"
     CDS             6620..7132
                     /locus_tag="IVY71_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7985527.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7388..7936)
                     /locus_tag="IVY71_001469"
     CDS             complement(7388..7936)
                     /locus_tag="IVY71_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7985528.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8025..9206)
                     /locus_tag="IVY71_001470"
     CDS             complement(8025..9206)
                     /locus_tag="IVY71_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7985529.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9442..10950)
                     /locus_tag="IVY71_001471"
     CDS             complement(9442..10950)
                     /locus_tag="IVY71_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7985530.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11109..11300
                     /locus_tag="IVY71_001472"
     CDS             11109..11300
                     /locus_tag="IVY71_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985531.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11503..12420
                     /locus_tag="IVY71_001473"
     CDS             11503..12420
                     /locus_tag="IVY71_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7985532.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12413..13486
                     /locus_tag="IVY71_001474"
     CDS             12413..13486
                     /locus_tag="IVY71_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985533.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13634..14152
                     /locus_tag="IVY71_001475"
     CDS             13634..14152
                     /locus_tag="IVY71_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985534.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14133..14525
                     /locus_tag="IVY71_001476"
     CDS             14133..14525
                     /locus_tag="IVY71_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7985535.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14601..15536
                     /locus_tag="IVY71_001477"
     CDS             14601..15536
                     /locus_tag="IVY71_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7985536.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15669..16448
                     /locus_tag="IVY71_001478"
     CDS             15669..16448
                     /locus_tag="IVY71_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7985537.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16441..16758
                     /locus_tag="IVY71_001479"
     CDS             16441..16758
                     /locus_tag="IVY71_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7985538.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17002..18612
                     /locus_tag="IVY71_001480"
     CDS             17002..18612
                     /locus_tag="IVY71_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7985539.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18769..19617
                     /locus_tag="IVY71_001481"
     CDS             18769..19617
                     /locus_tag="IVY71_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7985540.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6350 a         3513 c         3956 g         5830 t
ORIGIN      
        1 ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct
       61 ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc
      121 agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg
      181 tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca
      241 gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa
      301 ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa
      361 tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta
      421 gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg
      481 atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca
      541 gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc
      601 aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa
      661 gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta
      721 gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc
      781 atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat
      841 tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg
      901 aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta
      961 tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag
     1021 tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa
     1081 acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt
     1141 tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg
     1201 aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt
     1261 attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct
     1321 atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag
     1381 tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg
     1441 ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta
     1501 caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt
     1561 tacagattgg tacagagctt cataaagaag ggccggaaat tttttctaga atcataaaag
     1621 aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat
     1681 tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa
     1741 aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg
     1801 ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga
     1861 gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa
     1921 gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca
     1981 cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa
     2041 aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac
     2101 acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc
     2161 ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta
     2221 tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc
     2281 tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc
     2341 tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat
     2401 gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac
     2461 agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc
     2521 gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat
     2581 agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt
     2641 aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc
     2701 actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc
     2761 aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc
     2821 catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc
     2881 tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct
     2941 attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga
     3001 tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt
     3061 tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct
     3121 gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg
     3181 aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt
     3241 tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga
     3301 agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct
     3361 tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa
     3421 gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat
     3481 gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa
     3541 atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca
     3601 gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca
     3661 gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc
     3721 cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg
     3781 cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag
     3841 tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag
     3901 aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc
     3961 aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc
     4021 gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat
     4081 tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga
     4141 tgtactttcc tctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag
     4201 gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat ttggtagggt
     4261 tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt
     4321 atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta tatttcgggt
     4381 taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg
     4441 catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg
     4501 ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag
     4561 cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt
     4621 cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc
     4681 ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta
     4741 tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatccatcag tcaatctgaa
     4801 aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct
     4861 gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga
     4921 aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga
     4981 gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac
     5041 gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt
     5101 gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt
     5161 tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt
     5221 tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat
     5281 tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt
     5341 catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata
     5401 ttttccaaaa gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt
     5461 ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga
     5521 gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca
     5581 gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga
     5641 ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat
     5701 aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa
     5761 ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc
     5821 aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct
     5881 gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt
     5941 gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt
     6001 gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa
     6061 attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca
     6121 gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct
     6181 gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct
     6241 atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac
     6301 agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa
     6361 aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg
     6421 gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg
     6481 catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct taaatattat
     6541 tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga
     6601 atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt
     6661 ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca
     6721 aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta
     6781 tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcag gcgtggatcc
     6841 cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca
     6901 tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca
     6961 tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc
     7021 taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga
     7081 atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa
     7141 tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa
     7201 gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct
     7261 caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca
     7321 tcgagaggtc gtgacatagc ttttgtcacg atctctcggc ttatcctacg agtattattc
     7381 gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat
     7441 cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt
     7501 ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc
     7561 gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat
     7621 actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca
     7681 gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact
     7741 ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat
     7801 cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt
     7861 ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata
     7921 catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga
     7981 aaatatttcc aaagctttta aaagtaactt tgcttttttg tattttatac cagcaagcca
     8041 gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt
     8101 tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg
     8161 atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca
     8221 gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt
     8281 ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag
     8341 gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg
     8401 aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg
     8461 atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct
     8521 ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt
     8581 gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa
     8641 tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca
     8701 ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa
     8761 acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc
     8821 ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata
     8881 tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag
     8941 aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca
     9001 aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt
     9061 gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc
     9121 aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa
     9181 ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt
     9241 acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
     9301 aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa
     9361 cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca
     9421 acatttataa gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc
     9481 gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac
     9541 agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat
     9601 tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc
     9661 caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga
     9721 caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc
     9781 cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc
     9841 gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata
     9901 gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat
     9961 cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt
    10021 ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac
    10081 gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt
    10141 accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata
    10201 gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt
    10261 tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca
    10321 tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata
    10381 cagttggttc aagcaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac
    10441 gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt
    10501 ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct
    10561 ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac
    10621 actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc
    10681 ataagtaatc ccataatcac cacggatctt attacgttca atatctccag catagacgca
    10741 aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc
    10801 aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact aacttcccac caaattctaa
    10861 atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata
    10921 tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt
    10981 tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa
    11041 atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa
    11101 ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga
    11161 tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt
    11221 atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa
    11281 gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc
    11341 agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt
    11401 atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt
    11461 attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca
    11521 aatgcaaatt atgagccaga cgaactagag gagacgaacg cggatcagga tacaggacaa
    11581 gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta
    11641 gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt
    11701 ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc
    11761 gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa
    11821 caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat
    11881 cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat
    11941 gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag
    12001 ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa
    12061 gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa
    12121 atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta
    12181 ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag
    12241 caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc
    12301 agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat
    12361 tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga
    12421 acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa
    12481 cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct
    12541 agaggaaaca aaaatacatg aaaaagaaaa agtaactccg acgaatacga atactgcaga
    12601 aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga
    12661 tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat
    12721 gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat
    12781 attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta
    12841 tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac
    12901 atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt
    12961 ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa
    13021 cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg
    13081 acactggtca gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt
    13141 gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga
    13201 caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt
    13261 ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga
    13321 aactacttat caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa
    13381 agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt
    13441 tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga
    13501 ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa
    13561 ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg
    13621 gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg
    13681 tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt
    13741 tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat
    13801 tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta
    13861 tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct
    13921 atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt
    13981 ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc
    14041 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
    14101 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
    14161 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
    14221 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
    14281 gtcaaaactt ttcggaaaaa gaagtggtct tcaaaaaagg cgatgattta ctgagtattt
    14341 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
    14401 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
    14461 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
    14521 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
    14581 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
    14641 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
    14701 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
    14761 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
    14821 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
    14881 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
    14941 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
    15001 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
    15061 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
    15121 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
    15181 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
    15241 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
    15301 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
    15361 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
    15421 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga
    15481 agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg
    15541 tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc
    15601 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
    15661 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
    15721 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
    15781 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
    15841 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
    15901 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
    15961 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
    16021 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
    16081 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
    16141 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
    16201 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
    16261 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
    16321 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
    16381 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
    16441 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
    16501 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
    16561 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
    16621 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
    16681 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
    16741 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
    16801 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
    16861 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
    16921 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
    16981 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
    17041 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
    17101 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
    17161 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
    17221 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
    17281 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
    17341 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
    17401 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
    17461 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
    17521 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
    17581 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
    17641 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
    17701 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
    17761 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
    17821 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
    17881 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
    17941 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
    18001 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
    18061 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
    18121 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
    18181 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
    18241 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
    18301 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
    18361 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
    18421 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
    18481 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
    18541 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
    18601 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
    18661 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
    18721 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
    18781 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
    18841 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
    18901 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
    18961 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
    19021 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
    19081 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
    19141 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
    19201 cttgcacata tcgatcgaat ccgagataat acctatgctt atctgattgt agatgatttg
    19261 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
    19321 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
    19381 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
    19441 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
    19501 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
    19561 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
    19621 cttttattca aaaaacatac caaaaatat
//
LOCUS       DACCXW010000051        19085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCXW010000051 DACCXW010000000
VERSION     DACCXW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.051"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            18..572
                     /locus_tag="IVY71_001482"
     CDS             18..572
                     /locus_tag="IVY71_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7985541.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(745..1218)
                     /locus_tag="IVY71_001483"
     CDS             complement(745..1218)
                     /locus_tag="IVY71_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7985542.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1230..1766
                     /locus_tag="IVY71_001484"
     CDS             1230..1766
                     /locus_tag="IVY71_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7985543.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1806..2177
                     /locus_tag="IVY71_001485"
     CDS             1806..2177
                     /locus_tag="IVY71_001485"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7985544.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2461..3732
                     /locus_tag="IVY71_001486"
     CDS             2461..3732
                     /locus_tag="IVY71_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP7985545.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3977..5002
                     /locus_tag="IVY71_001487"
     CDS             3977..5002
                     /locus_tag="IVY71_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7985546.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5296..6405
                     /locus_tag="IVY71_001488"
     CDS             5296..6405
                     /locus_tag="IVY71_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7985547.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6488..7384
                     /locus_tag="IVY71_001489"
                     /pseudo
     CDS             6488..7384
                     /locus_tag="IVY71_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7400..8224
                     /locus_tag="IVY71_001490"
     CDS             7400..8224
                     /locus_tag="IVY71_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7985548.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8256..9566
                     /locus_tag="IVY71_001491"
     CDS             8256..9566
                     /locus_tag="IVY71_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP7985549.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9714..10973
                     /locus_tag="IVY71_001492"
     CDS             9714..10973
                     /locus_tag="IVY71_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7985550.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10983..11855
                     /locus_tag="IVY71_001493"
     CDS             10983..11855
                     /locus_tag="IVY71_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7985551.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11867..12697
                     /locus_tag="IVY71_001494"
     CDS             11867..12697
                     /locus_tag="IVY71_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7985552.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12737..14920
                     /locus_tag="IVY71_001495"
     CDS             12737..14920
                     /locus_tag="IVY71_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7985553.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14981..16201
                     /locus_tag="IVY71_001496"
     CDS             14981..16201
                     /locus_tag="IVY71_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP7985554.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16198..17658
                     /gene="gtfA"
                     /locus_tag="IVY71_001497"
     CDS             16198..17658
                     /gene="gtfA"
                     /locus_tag="IVY71_001497"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP7985555.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17735..18841
                     /locus_tag="IVY71_001498"
                     /pseudo
     CDS             17735..18841
                     /locus_tag="IVY71_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         6480 a         2909 c         3723 g         5973 t
ORIGIN      
        1 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
       61 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      121 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      181 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      241 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      301 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      361 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      421 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      481 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      541 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
      601 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
      661 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
      721 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
      781 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
      841 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
      901 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
      961 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1021 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1081 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1141 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1201 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1261 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1321 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1381 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1441 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1501 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1561 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     1621 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     1681 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     1741 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     1801 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     1861 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     1921 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     1981 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2041 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2101 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2161 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2221 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2281 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2341 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2401 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2461 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2521 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     2581 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     2641 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     2701 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     2761 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     2821 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     2881 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     2941 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3001 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3061 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3121 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3181 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3241 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3301 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3361 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3421 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3481 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3541 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     3601 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     3661 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     3721 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     3781 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     3841 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     3901 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     3961 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4021 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4081 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4141 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4201 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4261 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4321 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4381 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4441 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4501 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4561 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     4621 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     4681 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     4741 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     4801 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     4861 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     4921 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     4981 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     5041 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     5101 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     5161 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     5221 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     5281 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5341 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5401 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5461 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5521 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     5581 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     5641 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     5701 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     5761 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     5821 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     5881 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     5941 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     6001 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     6061 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     6121 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     6181 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6241 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6301 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6361 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6421 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6481 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6541 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     6601 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     6661 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     6721 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     6781 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     6841 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     6901 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     6961 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     7021 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     7081 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     7141 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     7201 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7261 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7321 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     7381 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7441 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7501 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7561 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     7621 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     7681 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     7741 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     7801 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     7861 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     7921 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     7981 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     8041 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     8101 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     8161 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     8221 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8281 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8341 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8401 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8461 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8521 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     8581 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     8641 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     8701 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     8761 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     8821 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     8881 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     8941 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     9001 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     9061 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     9121 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     9181 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9241 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9301 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9361 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9421 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9481 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9541 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
     9601 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
     9661 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
     9721 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
     9781 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
     9841 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
     9901 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
     9961 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    10021 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    10081 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    10141 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    10201 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10261 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10321 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10381 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10441 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10501 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10561 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    10621 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    10681 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    10741 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    10801 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    10861 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    10921 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    10981 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    11041 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    11101 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    11161 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    11221 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11281 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11341 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11401 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11461 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11521 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    11581 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    11641 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    11701 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    11761 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    11821 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    11881 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    11941 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    12001 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    12061 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    12121 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    12181 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12241 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12301 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12361 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12421 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12481 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12541 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    12601 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    12661 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    12721 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    12781 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    12841 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    12901 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    12961 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    13021 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    13081 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    13141 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    13201 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13261 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13321 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13381 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13441 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13501 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13561 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    13621 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    13681 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    13741 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    13801 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    13861 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    13921 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    13981 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    14041 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    14101 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    14161 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    14221 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14281 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14341 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14401 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14461 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14521 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    14581 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    14641 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    14701 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    14761 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    14821 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    14881 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    14941 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    15001 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    15061 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    15121 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    15181 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15241 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15301 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15361 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15421 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15481 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15541 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    15601 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    15661 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    15721 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    15781 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    15841 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    15901 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    15961 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    16021 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    16081 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    16141 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    16201 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16261 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16321 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16381 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16441 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16501 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16561 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    16621 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    16681 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    16741 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    16801 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    16861 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    16921 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    16981 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    17041 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    17101 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    17161 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    17221 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17281 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17341 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17401 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17461 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17521 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    17581 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    17641 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    17701 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    17761 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    17821 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    17881 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    17941 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    18001 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    18061 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    18121 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    18181 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18241 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18301 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18361 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18421 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18481 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18541 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    18601 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    18661 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    18721 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    18781 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    18841 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    18901 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    18961 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    19021 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    19081 aaatt
//
LOCUS       DACCXW010000052        18782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCXW010000052 DACCXW010000000
VERSION     DACCXW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.052"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            95..1522
                     /locus_tag="IVY71_001499"
     CDS             95..1522
                     /locus_tag="IVY71_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7985556.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1614..1979)
                     /locus_tag="IVY71_001500"
     CDS             complement(1614..1979)
                     /locus_tag="IVY71_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7985557.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1976..2341)
                     /locus_tag="IVY71_001501"
     CDS             complement(1976..2341)
                     /locus_tag="IVY71_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7985558.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2533..2781)
                     /locus_tag="IVY71_001502"
     CDS             complement(2533..2781)
                     /locus_tag="IVY71_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985559.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2780..2908
                     /locus_tag="IVY71_001503"
     CDS             2780..2908
                     /locus_tag="IVY71_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP7985560.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2965..4659
                     /locus_tag="IVY71_001504"
     CDS             2965..4659
                     /locus_tag="IVY71_001504"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP7985561.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4726..4932
                     /locus_tag="IVY71_001505"
     CDS             4726..4932
                     /locus_tag="IVY71_001505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985562.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5310..6443
                     /locus_tag="IVY71_001506"
     CDS             5310..6443
                     /locus_tag="IVY71_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP7985563.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6474..6794
                     /locus_tag="IVY71_001507"
     CDS             6474..6794
                     /locus_tag="IVY71_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985564.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6823..7287)
                     /locus_tag="IVY71_001508"
     CDS             complement(6823..7287)
                     /locus_tag="IVY71_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7985565.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7419..8672)
                     /locus_tag="IVY71_001509"
     CDS             complement(7419..8672)
                     /locus_tag="IVY71_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7985566.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8701..9018)
                     /locus_tag="IVY71_001510"
     CDS             complement(8701..9018)
                     /locus_tag="IVY71_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7985567.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9044..9364)
                     /locus_tag="IVY71_001511"
     CDS             complement(9044..9364)
                     /locus_tag="IVY71_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7985568.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9828..11243)
                     /locus_tag="IVY71_001512"
     CDS             complement(9828..11243)
                     /locus_tag="IVY71_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7985569.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11260..12576)
                     /locus_tag="IVY71_001513"
     CDS             complement(11260..12576)
                     /locus_tag="IVY71_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7985570.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12726..13424)
                     /locus_tag="IVY71_001514"
     CDS             complement(12726..13424)
                     /locus_tag="IVY71_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP7985571.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13713..14702)
                     /locus_tag="IVY71_001515"
     CDS             complement(13713..14702)
                     /locus_tag="IVY71_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP7985572.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14782..15615)
                     /gene="msrA"
                     /locus_tag="IVY71_001516"
     CDS             complement(14782..15615)
                     /gene="msrA"
                     /locus_tag="IVY71_001516"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7985573.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15790..17424)
                     /locus_tag="IVY71_001517"
     CDS             complement(15790..17424)
                     /locus_tag="IVY71_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985574.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17411..18781)
                     /locus_tag="IVY71_001518"
     CDS             complement(17411..18781)
                     /locus_tag="IVY71_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7985575.1"
                     /translation="MSFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIA
                     LTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLS
                     KQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISD
                     SIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWF
                     LFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSI
                     AAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVG
                     ESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNV
                     DVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEIN
                     KKNENK"
BASE COUNT         5938 a         3704 c         3140 g         6000 t
ORIGIN      
        1 atgctttttc agaaattaac tgatgatttc aaaaagaaaa catgtcataa tatttcccat
       61 attgtgcaaa aaaatacaag gcggaggctc tactatgatt gaagaatata ttgagaaaaa
      121 tattttaaga cagctgtttt tatgtgggca attctatgta aataaagaag tcaatctaga
      181 aaagctctcc aatctcctgc atgtttgcaa aaccacttta ctcaatgata tcaataacat
      241 aaaaaaagaa tttgaagaac agatcgctta tacacatcgg gaaaaggacc gttacacttt
      301 atatttttca gaacatatac ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt
      361 gtttttaaaa acttgtctgc tttatttgga agaagatgaa cctgattatc ttcaattaac
      421 tgaatgtgaa ttcatctctg tcagcaaagc ttattcatta aaaaaacaag tattagctta
      481 ttttaatgac tgcgggatcg aaatcgaccg ctactcccct cgattcactg aaatggaaag
      541 acgtctgctg cttcttaatg tgtcgtatcg tttaggcggt tttaattctt gggagctacc
      601 tgaatcgttt tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa atagtggcag
      661 attttatgat aaagaaaata aagaaatctt atcgattggt tttgctatca gtttcctacg
      721 ccagcaagtg tgtgctgtga caatagattc aaaatttatc gaagaaatca aaaaacgtcc
      781 tatttacaat tatgtcgaat cagcatggga aaacactgat ttccaaacct attataaaaa
      841 agaagaattc gcctttattt tgacactatt caatctttgt aattatggtt tccattctta
      901 tcagttgatt gcggaagact ttcaacagct acaccaagtg tttattgaca atactcccga
      961 aataaaagaa ctagttgcta cctttgaatc tcacttcaat caagaattat tcggaaacca
     1021 accgttcgaa cgtgcgctga ttcatctgat gcgttcggct tgggataact atcaattatt
     1081 catgccagaa aaattttatc ttttaaatga ggaacagaca aatttatata aagaagtcca
     1141 gacaattttt tccagctggt ccagccagtt accttatgat ctacggttga atcctaattg
     1201 catgcgggct tttgtgatcg aattgagcgg aattctccgt ctgacaaaag aacatctgac
     1261 tatctatatc gtcacaaatt cggatgttca ttatctgatt tatcgggaag ccttggaagc
     1321 tgtaactact ttcgattttc aagttgcacc aacaatttat tcaagtatat ctgatataaa
     1381 gaagtatgcg cagcagtcct caaaccgggt attatgtgaa cgtacattat atacaccaga
     1441 tgcggtccaa tatgaaaata ttattcctat ttcgatcaat acgatcgatc gagctatcat
     1501 ttcagctgtg caaaacaaat aaggggctgt gacaaaaatc atgtcacagc ctcttatttg
     1561 ttgaagatgc ccatttctgt aacaatctct tgatacacgt caggctcaca acctcactta
     1621 ttttggacat ctacgggtcc gatctcttta tttaaaaaat aagaaatgat ggttcggata
     1681 actaccaacg ccgctagttg gaaaatatcc tgcaaggtag gtttgataat cgattcgatg
     1741 atcccagctg aaacaagaat ttccaaactt agaagtatat agcctcccaa catcttttta
     1801 atagcattgt tctcgctgac tcggtcgctt tgcacttttg tatggacaaa taaatatttc
     1861 aaaaaatcct tgattgatag acaaacaccc caaaccaata aacagatagc aaagatatca
     1921 agtcccagta caagtttttc gaaatatgga agaagaaact gcatcaactc tgtcgtcatc
     1981 ctacctctcc tcttctttgt ttttctttgt cttctatttc ctgattcaag aaataagaaa
     2041 tgactgttcg aatgataata ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga
     2101 taactgactc gatgatatca gcaacgacta gtacttccaa gcttaacaaa atataacttg
     2161 ctagcagttt tcttgtatct gcattacgag tattacgttc tgcatagttt ttcttgacat
     2221 gagaccaatc aaaaagtttg aatagaacaa gaacgatccc gacaattaag ataccgatag
     2281 aacaaatatt taaaatagca atgatccatt ctagaaattc aattaaattt gagcgaaaca
     2341 tatacgctac ctactttctt ttaatttgtt cctattcttt tattgtaaaa gaaagttacc
     2401 ttgttcgtct atttaaaagt tgttctaatg ataacggttc gcttaaattt ttctttttca
     2461 ctctcccatt tttctccctc catccataaa tcgtggaagt atccaaaaca acgtagcaag
     2521 ttaaaataag gcttagtccg tcatcagctt ttcaaagaag cactgtctcg gtgctttttt
     2581 tgtacccaaa tttctaaaaa tagaaccata cacctcctca aatcttttct tcttacctac
     2641 cctattttta tttacaggaa aatcagacac ttcttctttt ttgtcaaaat ttcctttttc
     2701 ttctcgtcct atcatttggc attctaataa aaacgaacta tccttggata tagaaagaga
     2761 aaacaaggag gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt
     2821 gcttgccctt ggcgctgcat atctaggaaa aaagacagga ctattcgaag atgacagtca
     2881 tttatatgat gaattcgaat caatctagca tcctatttag ttgaatcact acggacatac
     2941 ttggaagaca ttggaggaat cacaatgaga aaaagtaaag caataatgat tggtgccggt
     3001 attgccaata tggcagcggc tgtttatttg attcaagaag gcaaatggaa aggggatcag
     3061 atcacctttt attcattgga tgatcacggt tcaaatgatg gtgcaccgac cgacactgta
     3121 gtggatgaat attggaataa aaaccatcca ttagaaaacc aaaaaggcta tattgctcga
     3181 ggcggtcgca tgcttaatta ccgtacctat gtagatttga tggatctgtt aagtcgaata
     3241 ccttctgtta ctgagccagg gatgactgct gaagaagata cgagagattt tgatgcaaaa
     3301 caccgaacct ttgacaaagc acgtttgatg gaaggtggca aaggaatcat tgatggcgga
     3361 aaactgggat tcaataataa agaccgtatt cttctgacta aactgattgc tatgcctgat
     3421 tcagaagaag aaaaactaga caatatcact atcgaagaat atttcaaaga tgatccacac
     3481 ttttttgaaa caaacttctg gtttatgtgg gaaacaacat ttgctttcag aacacgtagt
     3541 tctgctcaag aattgcgtcg ctatatgcat caaatgattt atgaattcac tcaaatcgaa
     3601 catctagtgg gcgtcaatcg aacacgttac aatcaatacg aaagtattat gcttcctttg
     3661 atcgagtact tgaaagaaca aggatgcaaa atcattttga accgccgtgt cactgactgg
     3721 gaattcaaag aaacaccgat gcaagatgaa atcactgtga ctggtttgaa aattaccaat
     3781 acactgactc aagaagaaga atacgttgtg gtcgatgaag atacagcagt tattttcact
     3841 aatgggtcca ttacagattc agctacttta ggagattacg aaacaccagc tgtagaaaat
     3901 atggattatg gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg
     3961 ggaaatcctg acaaattctt tgctgatcga gatgcaagcg aatgggtcag ctttaccctg
     4021 acgacgaaaa accatttgtt gttgaatgaa atcacccgta tcacgactca agaaccagga
     4081 aatgccttga attcatttat ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc
     4141 aatatgtcta ttgtcgtgca tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca
     4201 gttatttggg gttatttcct ttatccaaga cgacgtggcg aatttgtgga taaacagtat
     4261 attaaaatga atggaaaaga aatgctagaa gaactgattg gacaactttc aaaagtagat
     4321 cctggaccag tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt aataaataat
     4381 atccctgtct atatgcctta tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga
     4441 ccaaaagtca ttcctaaaca ttcgaccaat ctggccttta caggagaatt tgtcgagcag
     4501 ccttatcaga tgatctttac tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc
     4561 cattttgcgg gtattcctat gtcccgtctt gtcaaaacac cgcgttacga caaagatatt
     4621 cctactctag ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg tgaaaaagct
     4681 gccctgcatc aagtaataag aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg
     4741 ttggacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
     4801 gctgccctgc atcaagtaat aagagcagcc aagctaaaac agcttcttat gtttttgact
     4861 tggttagact tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttatgaa
     4921 aaaggcgttt agtttcgagt atgaagaaaa attttgtgaa aatggttcct cacgtttttg
     4981 cataaatttg acttaatacc gagaaactga tcccttctgt cataacctct ttttatttat
     5041 gtctgtttct ttttgaaaac aactattttt tcaacgttta ctctcttgcc aatccaacat
     5101 catctaacgg atacaactat ttcaatgatt accattacac taaaaaatcg gaaacacacc
     5161 tctccgaaca caaatacata agactataga atgtttcatt taagaaaaaa agcgtttcat
     5221 tatcaaaaag gtaagatctc ctttaaaatg aaattatctg tagtactaat aatgcagaca
     5281 attttatgaa tctgaaagga gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga
     5341 aaatggaaat cagaaacgta gatgatccaa aaatattaga accaacagat gcaatcatcc
     5401 gtatcacagc aacagccatt tgcggctccg atctgcatct ttaccatcat ggtgaagcag
     5461 ttttggaaaa agggtatgtt gtaggacacg aaccaatggg aatcgttgaa gaagtgggat
     5521 ctcaagttca aacattgaaa aaagggcaac gtgtggttat cccttttaat attagttgtg
     5581 gtcattgtca tttctgcctg aatcacatgg aaagccaatg tgatgaatca aatccagatc
     5641 agttatatgg cgggcttttc ggatttggca agttgaacgg taatcattgg ggtggtcaag
     5701 cggaatatct aagagttcct tatgcagact ctacttcatt tgttgtacca gataatgatc
     5761 ttcctgatga aaaagtttta tttctatcag atattttgcc cacagcttat tggagcgttg
     5821 aaaacgcagg agtaaaaaaa ggcgatacag ttgtgattct aggttctggt ccagtcggtc
     5881 tattcgctca aaaattcgct gttatggctg gagcagagcg cgtaatcgca gtagatccag
     5941 tacagcaccg tctagataaa gcggctaatt acaatggggt tgaaacttat ttcttagaag
     6001 acacggcaca agctggaaca gaattgtatg aactggctaa aggtggcgca gatgtcatta
     6061 tcgattgtgt aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa
     6121 gcttacaaag cggcacaatc tctccattac agatggcatc gcaagctgtc aaaaaatttg
     6181 gaacagtcca agtaactggt gtttatatga ctccagcttc ttcttacccg ctgcaagaat
     6241 tttttatgcg ggatatcgct gtcaagcatg gacaggcacc aattatccat ctaatgccaa
     6301 aaatttacgc aatgattgca gaaggactat tcgatcctac acaaatcatt actcattcgc
     6361 tgccactatc agaagcagca caagcatatg agatctttga caaaaaagaa gataaaaata
     6421 taaaagttgt tttgaaacct tgaattcagt aaaaaataca ggtgattttc tttatgccca
     6481 aaatcaaacg aaatacccat gagatcgaaa taggaaaaga agaagatatc gaaatacgag
     6541 gacgtcgctt ccgcttctat ttccaaaatc gttacacatt gatctcactg gctgtcgatt
     6601 tattaaccgg gattttctat atttttggaa gccttgcttc cttaactaaa atccccgatc
     6661 gatatggcac ctatttttat ttggctggtg ctattttctt gacgattcgt cctattctca
     6721 gaatcgtaag gaacattttt atttacgacg aaaaaaaagc aatgaagaga acaagcaaaa
     6781 aaaaagaggc atgagaattt ttcaaatctc atgcctcttt tttcacccta tcgtatcact
     6841 ttgatacaga cgagcggtcg tcccactttc ttccttgacc attgtcaaaa atgtccactc
     6901 gtatcgttca taaaacgtgg tatgatctgt aattaagtaa agtttttcgt acccaagttg
     6961 atgcatttct tttctgattt ccgctaatat ccgcttggct atcccctgct tacgcttttc
     7021 cggttcaaca aacagggcac aaagatttgg tgtcagatca gggcggttat ggaagtcgtt
     7081 ttcaatgacg cccgctcctc cgacgatggt ttctttttca tctaagacca cataccattg
     7141 aggaatactt tccttattag cgattccagt acgaatactt tcctcataca caacagcagg
     7201 aactccccat tttgatgaaa accaattcgc gctttgtttt actaattctg ggtgttctct
     7261 taatttgatc cagttatatg taaccaatgt ttaaacctcc gactaaaata aaacgtttac
     7321 aaaaattata caagtcttta tgaaaattag caatgacaac aatagtgatg taaaaaaaaa
     7381 aacggaaaac aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt
     7441 cttctttctt ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc
     7501 aaattgacag cggctagtac accacctgca atactttttg ttgccaatac accgccaata
     7561 attggtggag ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc
     7621 gcaaagtaag caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc
     7681 gggttcaaca cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc
     7741 gcaatcgaca agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata
     7801 gctaacagta aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa
     7861 ggtttgttca cgataaacgg tgcgctatgt ccagctgcga ttgcattaac gtttgcttca
     7921 atggctggcg cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca
     7981 aagaaccata agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca
     8041 agtcccataa atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg
     8101 aatgcagcaa aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt
     8161 aatgaagcaa atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat
     8221 ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca
     8281 ataaacaaac cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa
     8341 gaaatcacac ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc
     8401 aagaaagcaa tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat
     8461 cctttccatg attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt
     8521 aagttattga tcattactgc taatgctcct aagatcatta acggcatact caccataaat
     8581 gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa
     8641 tgtttctcca taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga
     8701 ttacgcgttc aatcgacgat acagatcaat gatctctttt gctaaatctg taaaagctat
     8761 agatgtcatc agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc
     8821 ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc
     8881 tgcatctttg attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc
     8941 ttccattgca tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag
     9001 atttttttga tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt
     9061 tttctgcttg gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta
     9121 catctaaagg aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta
     9181 cttgcggtcc taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg
     9241 aagcagatac agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt
     9301 ttgtgactag gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt
     9361 ccattttaaa cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac
     9421 tttaaaagcg ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac
     9481 acactaaaaa agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt
     9541 taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt
     9601 attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt
     9661 tttactttgc tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac
     9721 ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta
     9781 tttcaaataa ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt
     9841 tcgtcaggca aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt
     9901 ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta
     9961 aatgcattca ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt
    10021 gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg
    10081 taatcatctt gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata
    10141 ccgttttctg ttatcagcca agggatatta tgataatttt cttttagata catggctata
    10201 tcatacatga ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt
    10261 aaatcaaaga cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa
    10321 cgaggggcgc gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga
    10381 tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac
    10441 aactccagga actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta
    10501 taaaacaaat catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag
    10561 accatttcag gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc
    10621 tctactgctt tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg
    10681 ttttgttcat gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt
    10741 tcgttgaatg taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca
    10801 tacttagcat aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga
    10861 aaagctggga cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa
    10921 gagtcgatca tttcacttat atgctgagca tattcttcat ctactataca gttctcataa
    10981 tcagtaaaaa aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc
    11041 atcagatcca catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac
    11101 ccattatgcc aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct
    11161 tctttttttc ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa
    11221 ttttttggaa tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac
    11281 acgatttgct acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag
    11341 ttgcgtaata actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg
    11401 cattggccaa gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata
    11461 accaatcgtt ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag
    11521 catgatcggc ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga
    11581 taacttggtg atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa
    11641 accgatcgtg actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat
    11701 aatgttcggt acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa
    11761 aagcaacggt tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa
    11821 gacttgcgca ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa
    11881 tgggcgttgt acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat
    11941 gataaatccc gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa
    12001 actagcagaa acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt
    12061 cctcaaccag caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc
    12121 tttcgtatct gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt
    12181 cactaattca taaacagttg gattaacaga taaataagac atcactgcga tgataccagg
    12241 gaatacacca tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata
    12301 gatggtcaag aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc
    12361 taaaaaacgc aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga
    12421 aaacaatgtg ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt
    12481 tatcaccatg atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc
    12541 taatttatcc ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca
    12601 tctctttttg tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct
    12661 tttttaccaa atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg
    12721 aactgtcaat tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc
    12781 atatggacag tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg
    12841 atctctgtat gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta
    12901 aacaaagcaa ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta
    12961 gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct
    13021 cgaaaagaag tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt
    13081 gcatattccg ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag
    13141 tctcgtatca tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc
    13201 cctcgcttat cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa
    13261 gctagagccc gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat
    13321 tcatattcat tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg
    13381 atcttgtcag tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt
    13441 tcatctgatg tattttttcg taattattat aacacgtttg aatcatctga tgattttttg
    13501 tatacaaaat tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa
    13561 aagactagga taaaattcac tcctagcctt cgatcatttt tattttgaac gacaaaaaat
    13621 atgatgttca ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta
    13681 atatatggat aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat
    13741 tcttcttctg gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt
    13801 aaccgacttg ctccgaccag tacacttgta acagattttt gtcgcaaatt ccatgcaagg
    13861 gccatttgcg ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt
    13921 tccagagttg atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta
    13981 gaatcttcag gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg
    14041 aacgtgattg cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt
    14101 gcacgagaaa acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt
    14161 aaaatttcag tcattttttt tgtatcttct ccattatagt tggatatacc aatatataat
    14221 gcttttcctt gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa aggtgtatca
    14281 gggtctgggc gatgatgata gaagatgtca acataatcta gccccattct ttggagactt
    14341 tggtcacaac ttgaaatgat ggatttacga gagccccatt ccccatatgg cccttcccac
    14401 atatgataac ctgctttgct agaaataatc agttcatcac ggtaggcttg aaaatcttct
    14461 ctaaaaatac gcccaaagtt tttctctgca gctccagcag ggcctccata attattcgca
    14521 aggtcgaaat gggtaattcc catgtcgaat gcgcctctta aaatctctct ttgattatgg
    14581 ataggatcat aatcaccaaa attttgccat aatccgagag ataaaatcgg taatttcaaa
    14641 cctgaatgac cggatttacg atattccatt ttttcataac gtgcactatc tgcaatatac
    14701 aatttttctc ctcctcatca aatcttttta aaacgctttc ttttttctat ttttagttta
    14761 ctacatttta aagaaaagga gttaatcaaa atgatttttt cgtatatttt tttgccattt
    14821 gttttttact cgttgataga tcagaaattg attacgtgct tgctggattt ttttataacg
    14881 tttcggttgt tttttgtaaa attgctgatg atagttttct gctggccaaa acgtaccggc
    14941 tggttcgatt tttgtgacga ttggttggtg gaaagttcct gaactgtcta gtttctgttt
    15001 tgattgttcc gctaattctt tttgtcgttc gacttgataa aaaatcactg gacgatattg
    15061 cttccctcta tcttgaaatt gtccaaatgt atctgtggga tcagtaattt gccaatagat
    15121 tgtcagcaac tcgctataac ttatttctct tgtgtcataa atgatctcca cagcttccac
    15181 atgacctgtg taaccaccac ttacttgatc ataatttggt ttttcaacat gtccgcccgt
    15241 gtatcctgac aaaacagata ggatcccttt tttcgattca aacggctcga ccatacacca
    15301 gaaacatccc cctgcaaata ctgcacgttc ttgatagtca gcatcttgca tggcatgctt
    15361 ggaaaagtca tatttttgct ccttatcaaa tccttccgta atcaataaat aaaagtccat
    15421 tacatcaggc gtcagattca ttcggatagc taatggacgt agtgttgctt ctagttttga
    15481 taactgttct ttaacggata tattttctct tatgtcattc tttgtctgaa tcaatacttt
    15541 tcgttcccaa tctcgtgtat tcggattcaa aatcagatta taaagtgttt gaatgaatat
    15601 ttgttcctca tccatagatt ctcctccttt ccgaagaaga caagctagtg actttagctg
    15661 taatttatca taaagtgagg aaataagcga ttacttcatc cccctacaga tttttcgtat
    15721 aaaaaagaga gaatccttat cgacagattc tcctttttaa tcaatttatt ttataaatga
    15781 aaagttaatt taccttttat ggaaaactat tttaaaaaat aaacataaat atgcagttaa
    15841 tgtaacaaga taagaaataa tagtcgttac tgtataactt gtgcttactt taatatcagc
    15901 aactcctttg actccgctta tttgtaccaa tccattagca ttcacctgct cctttacagg
    15961 ttgatgattt atttctacat tcgttcctaa ataagcatat acaggaagtg tgattacttg
    16021 gctcttctca ttagttactc tatatatgat agaactacca gttgttttat aaggaaggtt
    16081 tacccattga ctgccaatct ttgcttcgtg ttttattata tgctccttat tctccaatga
    16141 agcgatagga gaataatcat ataaaaaata atccattcct ttagtggtat acgcctctac
    16201 tgtttctttg tttacaacaa tatgtcccgg ctgagaaatt attttttgat tgaaattttt
    16261 tttactagaa acagttaaag tgattaataa caaaacagtc ataaataaat aaaatagttc
    16321 tcttgtctta ttgtatcttc tatttttgaa aaatatcatc gttaatatca gagaagagaa
    16381 tattatttgc aagcttaaga tccgccaagg aaattgtaac aatttggctg gcgtattttg
    16441 cagaagatac catggcacca aactagttgt agctagccaa gtcaaacacg taccataata
    16501 aatctttttt tctatgtttg ttaatttttt aaataaaatc aggggaatac ataaagataa
    16561 cagaccaact agtcccattg agtaagtagt taagtcgttg ttcatagctt cagtcagact
    16621 atcgaacaca ttagatgcat gagcatataa atttgtcacg ctaggtttgt tgattttttg
    16681 gtagagcatt tgttcaaaca tcggatacca aaaagcactc gtcagaacca atgtcattcc
    16741 tgccgcacta aataattttt taaatatagc aataaaatct gcattcttga aacctttaga
    16801 aaccaaacga ataaaaataa aaatgataat taatgctgta ctcattaatg ttgatagtac
    16861 atggctgtac actagcaaac tcatactcaa tgctagcttt acagacggat acttttcttc
    16921 ttttccagag atgatttgat aagcataaaa taatacaggg ataacaagtg ttattgcgat
    16981 agcttcggca attgcggaac gatagtagat gtctactgtc cgataagcag agaaaatata
    17041 gagacaacta aataaaaatg ccgctttttt ttgttttaaa aaccgctcgc cataatgata
    17101 agctacgagt gttgtacaca agttcaataa ccaaacgtaa ataatatagc ttacaatcaa
    17161 attttttgtc atccaatata acattacagc tggaaaaaaa gtcagaaagg gataaaaata
    17221 atttacacca tatccagtat aattgaatgt ctcataatta atcggactgg taaaaatagt
    17281 cgataacccc ttgattcgag ataagtgaaa agaaagatct ccagtggtat aaatactctg
    17341 tgtatttttg aaaaagatga aagaatatat actactaact ataattaatc cactcaatat
    17401 tactactttt ttatttattt tcattcttct tgtttatctc ctttttagtc acatatcgag
    17461 aatctttcaa cttattaggt gcaaatcgtt taaaacttga atacaccact aaacaataag
    17521 tcagaatact gatccataaa gccatgagag cagttggagt atattttgtt gtaatttcta
    17581 tcttattttc tccagcattt acatccacat tcactaagcc atgtttttta taagagtcat
    17641 ttacgacttt accgtttact tttacctgtg tacctaaata agcatatact ggtactgtca
    17701 ctttttgctt tttgttgctt gccactgtgt aagtaatgtt attatctgca gctttatact
    17761 tccctttttc ccacgattct cctactttta cttcatgttt ttctaagtga ggcctttctt
    17821 ttatcgcttc tgttggggcg taatcgtata atcctcccat atctgatgtt gtataacgtg
    17881 ggagtgtttc ttcattgata acaatatgac cgtgttttga ttggataact tttgcgtaat
    17941 tttcttttgc tgctatagac gtgacgagta gaaacaccac agtagcgcca atgattaacc
    18001 atgaatattt tttattcata gtctttttca taaaaacaag cgttatgatc attgaagaaa
    18061 aaaagatttg gatacctaag atacgccaag gaaactggag taagcttatc ggtgtttttt
    18121 gaaacaaaaa ccatggaaat aaattagtta cgcataccca tgtagaaact acgccaacat
    18181 agatgatttt ttcttttttc tccatttctt tcagtatgaa taaaggaaaa atcaacgcaa
    18241 ccaatcctaa taaccccata gtgtatgttg taatatcgtt atttaacgct tgaatgatac
    18301 tatctgaaat agaaagtgct tctagctgca aatctgtttt gaagggactg tgtatttctt
    18361 gataattcat ctgctgcaac ataggatacc aaaaatagct agttaatgca caagtcaaaa
    18421 aaacagcctt tactaaattc acgaccacaa attttatatc gttttttatt tgcttgctta
    18481 aaaacaaacg aactataatc atacaagcaa tcaatgcact acacaatagt gtagttaaga
    18541 catgagtata cacaagtaga cttatactga cagataaagc gatgactttt ttttctctat
    18601 tgaatatcaa tccataagca taatataaaa cagctgggat aatcgtaaga gcgattgctt
    18661 ctgcaattgc ggatcgataa tagatatcaa ctgttcgata agttgcaaaa gtatataaac
    18721 aactaaataa aaaagcagca tctatctttt taaaaaactt ttctccataa taaaaactaa
    18781 tc
//
LOCUS       DACCXW010000053        18559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCXW010000053 DACCXW010000000
VERSION     DACCXW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.053"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            81..515
                     /locus_tag="IVY71_001519"
     CDS             81..515
                     /locus_tag="IVY71_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7985576.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(593..1126)
                     /locus_tag="IVY71_001520"
     CDS             complement(593..1126)
                     /locus_tag="IVY71_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7985577.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1220..2398)
                     /gene="mutY"
                     /locus_tag="IVY71_001521"
     CDS             complement(1220..2398)
                     /gene="mutY"
                     /locus_tag="IVY71_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7985578.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2391..3188)
                     /gene="recX"
                     /locus_tag="IVY71_001522"
     CDS             complement(2391..3188)
                     /gene="recX"
                     /locus_tag="IVY71_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7985579.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3295..4677
                     /gene="rlmD"
                     /locus_tag="IVY71_001523"
     CDS             3295..4677
                     /gene="rlmD"
                     /locus_tag="IVY71_001523"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7985580.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4851..5429)
                     /locus_tag="IVY71_001524"
     CDS             complement(4851..5429)
                     /locus_tag="IVY71_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7985581.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5751..6221
                     /locus_tag="IVY71_001525"
     CDS             5751..6221
                     /locus_tag="IVY71_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7985582.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6329..7555)
                     /locus_tag="IVY71_001526"
     CDS             complement(6329..7555)
                     /locus_tag="IVY71_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7985583.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8309..8509)
                     /locus_tag="IVY71_001527"
     CDS             complement(8309..8509)
                     /locus_tag="IVY71_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7985584.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8643..9251)
                     /locus_tag="IVY71_001528"
     CDS             complement(8643..9251)
                     /locus_tag="IVY71_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7985585.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9336..9806)
                     /locus_tag="IVY71_001529"
     CDS             complement(9336..9806)
                     /locus_tag="IVY71_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7985586.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9854..10768)
                     /locus_tag="IVY71_001530"
     CDS             complement(9854..10768)
                     /locus_tag="IVY71_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7985587.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10914..11717
                     /locus_tag="IVY71_001531"
     CDS             10914..11717
                     /locus_tag="IVY71_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7985588.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11895..12842
                     /locus_tag="IVY71_001532"
     CDS             11895..12842
                     /locus_tag="IVY71_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7985589.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12910..13203)
                     /locus_tag="IVY71_001533"
     CDS             complement(12910..13203)
                     /locus_tag="IVY71_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7985590.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(13231..13896)
                     /locus_tag="IVY71_001534"
     CDS             complement(13231..13896)
                     /locus_tag="IVY71_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7985591.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14036..14668)
                     /locus_tag="IVY71_001535"
     CDS             complement(14036..14668)
                     /locus_tag="IVY71_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7985592.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14675..15742)
                     /locus_tag="IVY71_001536"
     CDS             complement(14675..15742)
                     /locus_tag="IVY71_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7985593.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15739..16470)
                     /locus_tag="IVY71_001537"
     CDS             complement(15739..16470)
                     /locus_tag="IVY71_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7985594.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16639..17118)
                     /gene="greA"
                     /locus_tag="IVY71_001538"
     CDS             complement(16639..17118)
                     /gene="greA"
                     /locus_tag="IVY71_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7985595.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17254..18429)
                     /gene="mltG"
                     /locus_tag="IVY71_001539"
     CDS             complement(17254..18429)
                     /gene="mltG"
                     /locus_tag="IVY71_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7985596.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5529 a         3743 c         2982 g         6305 t
ORIGIN      
        1 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
       61 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
      121 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
      181 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
      241 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
      301 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
      361 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
      421 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
      481 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
      541 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
      601 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
      661 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
      721 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
      781 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
      841 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
      901 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
      961 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     1021 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     1081 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     1141 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     1201 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     1261 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     1321 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     1381 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     1441 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     1501 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     1561 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     1621 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     1681 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     1741 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     1801 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     1861 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     1921 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     1981 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     2041 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     2101 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     2161 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     2221 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     2281 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     2341 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     2401 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     2461 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     2521 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     2581 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     2641 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     2701 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     2761 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     2821 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     2881 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     2941 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     3001 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     3061 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     3121 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     3181 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     3241 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     3301 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     3361 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     3421 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     3481 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     3541 ggtggctgtc aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg
     3601 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     3661 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     3721 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     3781 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     3841 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     3901 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     3961 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     4021 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     4081 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     4141 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     4201 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     4261 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     4321 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     4381 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     4441 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     4501 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     4561 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     4621 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     4681 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     4741 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     4801 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     4861 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     4921 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     4981 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     5041 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     5101 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     5161 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
     5221 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
     5281 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
     5341 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
     5401 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
     5461 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
     5521 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
     5581 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
     5641 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
     5701 ttgctgtgag ccttcatggt aaactaatat tatagaaagg agttagtaaa atgaagatga
     5761 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
     5821 ggttgcttag cggaaatccc ggaaaatatt actattcttt gcaacttcca ccatttgcac
     5881 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
     5941 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
     6001 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
     6061 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
     6121 tgtggctgca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
     6181 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
     6241 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
     6301 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
     6361 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
     6421 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
     6481 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
     6541 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
     6601 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
     6661 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
     6721 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt
     6781 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
     6841 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
     6901 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
     6961 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
     7021 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
     7081 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
     7141 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcgtaggca tgatttaatt
     7201 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
     7261 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
     7321 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
     7381 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
     7441 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
     7501 tcagcaaaac gaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
     7561 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
     7621 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattatta ttttttaaaa
     7681 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
     7741 gcagttcgca atcgttttat ctttttttat gtattttttc cagaaaaagc gaacaacaag
     7801 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
     7861 gtctttgcct accgttcaga ttatcattcg atttcacgac aatttcttcc tatgactggt
     7921 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
     7981 tgactcgaac tagtgattgc attaccatca tcttttaatg gatagatatg ctcctcttca
     8041 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
     8101 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
     8161 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
     8221 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
     8281 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
     8341 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
     8401 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
     8461 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatatc
     8521 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
     8581 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
     8641 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
     8701 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
     8761 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
     8821 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
     8881 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
     8941 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
     9001 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
     9061 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
     9121 tcaattgctg aatttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
     9181 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
     9241 gtgtaagtca taaaaaaatt cctcctcaat gttttcttaa taatgtctac attacaaaga
     9301 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
     9361 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
     9421 gcaaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
     9481 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
     9541 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
     9601 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
     9661 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
     9721 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
     9781 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
     9841 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
     9901 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
     9961 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    10021 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacatcct
    10081 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcgata gcaggtgcac
    10141 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    10201 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    10261 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    10321 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    10381 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    10441 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    10501 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    10561 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    10621 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    10681 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    10741 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    10801 taacattttt ttgatatttc accaatcatt cagctgtttc cttaaagcac ttgtatttca
    10861 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    10921 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag gcgagaaatg
    10981 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    11041 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    11101 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    11161 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    11221 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    11281 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    11341 aacttcccta caccaatgaa tctttaaaaa acttaacagg taagcagctc cgttcttttt
    11401 taagtgaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    11461 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    11521 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    11581 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacta
    11641 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    11701 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    11761 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    11821 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    11881 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    11941 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    12001 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    12061 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    12121 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    12181 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    12241 aaaaggcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    12301 ggaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    12361 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    12421 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    12481 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    12541 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    12601 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    12661 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    12721 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    12781 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    12841 aatagctgaa aacaatattt tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc
    12901 tctctttttt tattgttttt taaagtgata ggttggctcc tccaggttgg gattataatc
    12961 aacaagtaaa tcatatcctt taaagaacca ttcatctgct tcctcgataa aaaaaagtgt
    13021 cccatcaatt tccactttct ttacaggatg ttctggctgt tcaactgaca ttccaatcga
    13081 aaaaccatca tgaacctctg ttttgccgta gacttttcca aaaaaacgta ttccgtaagt
    13141 ttctggcaga tcaatttctg agtcaaacca cttttgtgct tttggcgtta ccgttaattt
    13201 catcccaaca acctactttc tcttttcttt ctattgattc atcttatcgt ctagaatatc
    13261 cacatcgcct gtctctccaa taaccagtag attatcattt tcacgaacat attcatctgc
    13321 tgcaggagaa gcgataaccg tttgatctac tcttcgtatg gcgactactg ttaagtcaaa
    13381 acgttgcctg aaatttaatt cagctaatgt tttgttgaaa aatttaggat tggagactcg
    13441 gatttctgct aaagaatatt tatctgacaa ctctaggtaa tctaaaatat tctttgacac
    13501 taaattatgc gcaatcctta ttcccatgtc tcgctcggga tggacgacgc gatcagctcc
    13561 gatcttttca agtactctgg catgatactc attttgggct tttgctaaaa tatttggtac
    13621 gcccatttcc tttaccatca gagtaactaa gatactggct tgaatatctt ctccaatagc
    13681 gacaattaca tgatcgaaat ttcttatgcc taatgatcga agtgtcattt catcctgtgc
    13741 atttgccaca acagcatgag tggctatatt catatattca ttgacccgat cttcattact
    13801 atcaatagcc agtacttctt gaccagcttc gatcagtgtt cggcagatac ttccgccaaa
    13861 tcgtccaaga cctacaattg caaaattttg tttcaccgtt ttcctccttt agtatcttct
    13921 tttctcagta taacaaagcc tttacaaaaa caaaagcaag catcattatt gtaaaaaagc
    13981 caagaaaaat aatagttatt ttctcagctt ttgattatcc tctttatcat cgtttttatt
    14041 tcaccaaacc atgtttgaat gcatagatag cggcttgtgt acggtcttcc acttctagct
    14101 tagccaatat attggaaaca tgtgttttta ctgttttcag tgtaataaac aattcgtccg
    14161 ctatttcctg attacttttt ccttcagaaa tcagcattaa aatttcattt tcccgattcg
    14221 ttagttcttc atgtaataca ggctcatttc gccgactcat tttttccatc atttttgttg
    14281 tcacttctgg ttctaatact cgctcgccac gttgcgttgc ccttatagca tcagcaattt
    14341 catgtgcggt agaggtcttc aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa
    14401 ctttttcgtc atcaataaaa ctggttacga taatgatttt agcttctggc caatctttta
    14461 agattgcttt tgttgattcg ataccatcca tttcttccat gactaagtcc attaaaatga
    14521 cgtccggtcg aagtgtcatc gccttctcat agccttgtcg tccattttca gcttctccaa
    14581 ttacttcaat gtcttcttga atagataaat aaaaagagac gcctaagcgc accatttcat
    14641 ggtcatctac taataaaact tttatcatcc ttatctatgc ctccttcatc aaagggacct
    14701 tgatttctac acttgtacct tgacctttaa aactaatgat ttttactgtt ccaccaatac
    14761 cagctactcg ttctttgata ttgttcaatc cataacttcc cgttttcgtt tcattcatat
    14821 taaaccctgt tccgtcgtca ataatacgta acaaaagatt attgtctatt ttgtgcaaat
    14881 atacctctaa ttcattggct ttagcatgtc ttaatgtatt tgataacaat tcttgaacga
    14941 ttcggaacaa gtgatcctca atggaactag atagttttac atcttctaca tcccatttca
    15001 gcgaaatctg gattttattt tgcaattcct tcaataactg ctcgatacct tgctttaaac
    15061 ttttctcttc taaattgact ggacgtagat gaagaagcag cgcacgcatc tcagactggg
    15121 aagcgttgat gatctctgct accattttca actgcttgcg aaacatttct ggtgtctcgc
    15181 ttttttctgc ttgctccgtc aatgccgaca tcatcatcat tgctgcaaac aattgttgcg
    15241 aaactgaatc atgcagctca cgggctaatc gatgtctttc taattctaaa atttcttctt
    15301 tggtttgtcc atctacatat tggggacggc tcgttacaat ttgtaattca ctagatactt
    15361 caatcatttt ttcttttatc ttagtgattt ccttatcaat atctttgatg tataaatcgt
    15421 ccgatgcact tgggatcgga agaagcaaca atttggattc ataattgcca gaagaaagtg
    15481 cacgcagctt ttcttcgatt ttcccgtatt ttgtcttttg aataagtgat aataatagaa
    15541 agacaattaa tcctacacct aaggaaatgg ctaacaaatg gaagataagc ggaacatata
    15601 ataaacgtgc acgcagtaat tctccccacc aatgactttg attgctggca taaaaataag
    15661 tgaaaagtga aaatagaata ataaggaaag ccgcaatccc cgaataaaca gctagcatcg
    15721 ctctggatat ttttcccatc atattcgtat cacctcgaca tctccaatca aggtattagt
    15781 aataatcttc aatctacgag ggttttcatc ataatcactg ctgtagattt tgatttgttc
    15841 atttttcaaa gaaaattgct cttcttcaaa caagacattt cctactaatg tcgcatgctc
    15901 taatcggatg gctacgccca aaggtactaa gatccgtgtt ctgcctatcc cttttcttac
    15961 aatcacaata ttatcatctt ttggcaaaag cgtattgccc aaatctatga tagtatcacc
    16021 agaaatgacg gcgatgttga tatcgtccca ttcatagacg tcgtttccaa tacgttggtt
    16081 accgaataat tgctgtcttt tccgctcatt attatgtgtt tttaattgct ctgtttggat
    16141 catcatgatc tgtttctttc gccaaaaagc atttttggtt aaatctatcc ctgaaatctc
    16201 tacacctttc agaccaataa ataaaacggc aaatacagcc atcatccata aagcaggact
    16261 gttgactaag ctaaaaaaaa tgaccaaact tcctaatatc aattgaaaat tttgaaattt
    16321 agtacgagga tgtttgcgca tagctagata tatattgaaa ataccgaata tcactaaaag
    16381 caaaagtcca gtattgttta cgatttgcca aacagcaaat aacagcagta gtgcttcaac
    16441 taccacaaaa aaacgccaag aactattcat cgctctttct ccctcctgac acattctcat
    16501 ttcatcatac ctattttttt aactgttggc tataggactt tagatgtaag atcgtttgtt
    16561 gaaatctaaa atacacaaag agaaaaagat ctagagtgta tcatcagtaa gatgaactct
    16621 ctagatcttt cagtcagact acgcttgttc tactttgacg attttaacta acatatcgcc
    16681 acctggagta gcaatagtta cttcatcatt tttacgtttg ccaatcaatg cttgcgcaat
    16741 aggagaatca ttggaaattt tgccagaaaa cggatctgct tcagcgcttc cgacaatcgt
    16801 gtactcttct tcttctccat ctggtagttc gatgaatgta actgttttac caattgatac
    16861 ctcatctgca tctacaccgt cgttatcgat gatttgagca aaacgaatca tattttcaat
    16921 tgttgtgatt cttccttcaa caaatgcttg ttcatctttc gctgattcat attcagagtt
    16981 ttcggaaaga tctccgaaac cgcgggcaat tttgattcgt tcaatgattt ctccgcgttt
    17041 gactgttttt agttcttcta attctttttc taatttttct ttaccttcta gagtcattgg
    17101 ataaactttc tctaccatgt gtaacgtctc cttttttcga taaaataccg caaaaggtaa
    17161 tcttttgagg aatatagtcc aaaaaagatt accacgcttt tcttcaaatg taaatgtttt
    17221 tctaaaaatg atcaatgtct agagcctgtt tttctaagaa ttatttacat atttttccac
    17281 taattccata tgttcttcat aagttttagc gaaataaaca tttccagtgc tcgtatctgc
    17341 tacgaaataa taatagtcat tttctgctgg ttctaatact gccttgattg cctcttcgct
    17401 tggattgtca aaaggtccag gtccatagcc ggtattggta tacaaattat atggtgaatc
    17461 tacagctgta tcttcatacg tcactaattc tttgtgttca cctaatgcgt ataaaacaga
    17521 gatatctgac tgaagtggca tatttgcttt gattcggtta aagaagactt gtgcaatgtt
    17581 tttacgatca ttttctttta cgccttcttt ttcaactaga gaagctagag ttaaaacttc
    17641 ttgtacagtc aaatttttct gcttgatagc tgaataatag ttttgcatga ccgtattcgt
    17701 cttgttcacc atttcaatca ctagatcctt aagcgaagta ctcgtgtaat aatcatatgt
    17761 ggctgggaat aagtaacctt ccaaacggta ttttgtgttc tcagctttag atgcactttc
    17821 caacagttct gggaattttt ggtgcaactc gttaaagaaa gtctcatcat tcactaaatc
    17881 taaaaattct ttcttgtctt taccagttac cttcgctacc cgttcagcaa tctgatcgat
    17941 atcgtaccct tctggaatgg caattttccc atctgcaact tttgttggct cagaagtacc
    18001 gccttcttgc aactgtttgc caatctcgtc aagagtcata ttaggcgcta actgataata
    18061 gccagcttga aagccagtta aatttttaaa ttttgtataa taattgaaaa caatcccgct
    18121 tttaataata ttgtccttct ccaaaatctc accaatctgc ttgttagaag agccactcgg
    18181 gatctcgact tggactaatt ggtcatctga tttatctaaa ggcttcaacc cgctatctac
    18241 ataacgatac acggtaaatc caagaaatcc gccaataatc aataatgtaa gagcaatcac
    18301 taaaatgatc ttcctcacaa tcttatcttc ttttttacgc agatttctcc gcttgtcgat
    18361 tatatctgct gtagctgcct tactggcttc ttttttatct ttacttgggt gttgtttgtt
    18421 tcctgacaaa acaactcctc cttactatca agttcgtaac tttattcatt atacatgata
    18481 tttgaattgg cgtgaatgat tttatttaaa tattctatca aaaatgtaag tagtaaatcc
    18541 attactataa gattaaaat
//
LOCUS       DACCXW010000054        17294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCXW010000054 DACCXW010000000
VERSION     DACCXW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.054"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(60..605)
                     /locus_tag="IVY71_001540"
     CDS             complement(60..605)
                     /locus_tag="IVY71_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7985597.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(617..1309)
                     /locus_tag="IVY71_001541"
     CDS             complement(617..1309)
                     /locus_tag="IVY71_001541"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7985598.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1328..1612)
                     /locus_tag="IVY71_001542"
     CDS             complement(1328..1612)
                     /locus_tag="IVY71_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985599.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1625..2188)
                     /locus_tag="IVY71_001543"
     CDS             complement(1625..2188)
                     /locus_tag="IVY71_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7985600.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2416..3078
                     /locus_tag="IVY71_001544"
     CDS             2416..3078
                     /locus_tag="IVY71_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7985601.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3202..4398
                     /locus_tag="IVY71_001545"
     CDS             3202..4398
                     /locus_tag="IVY71_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7985602.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4499..5875)
                     /locus_tag="IVY71_001546"
     CDS             complement(4499..5875)
                     /locus_tag="IVY71_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7985603.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5912..6778)
                     /locus_tag="IVY71_001547"
     CDS             complement(5912..6778)
                     /locus_tag="IVY71_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7985604.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6867..7892)
                     /locus_tag="IVY71_001548"
     CDS             complement(6867..7892)
                     /locus_tag="IVY71_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7985605.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7910..8584)
                     /locus_tag="IVY71_001549"
     CDS             complement(7910..8584)
                     /locus_tag="IVY71_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7985606.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8768..9661)
                     /locus_tag="IVY71_001550"
     CDS             complement(8768..9661)
                     /locus_tag="IVY71_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7985607.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9735..11432)
                     /locus_tag="IVY71_001551"
     CDS             complement(9735..11432)
                     /locus_tag="IVY71_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7985608.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11425..12738)
                     /locus_tag="IVY71_001552"
     CDS             complement(11425..12738)
                     /locus_tag="IVY71_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7985609.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12738..16775)
                     /locus_tag="IVY71_001553"
     CDS             complement(12738..16775)
                     /locus_tag="IVY71_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7985610.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16788..17246)
                     /locus_tag="IVY71_001554"
     CDS             complement(16788..17246)
                     /locus_tag="IVY71_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7985611.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4835 a         3890 c         3012 g         5557 t
ORIGIN      
        1 gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt
       61 tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg
      121 ggatcgaaac ttgccacgga atctttgaaa atatggagtt tgtatcctag attatacgca
      181 tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg
      241 atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt
      301 ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag
      361 ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc
      421 ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga
      481 tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca
      541 gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg
      601 ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc
      661 gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt
      721 cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag
      781 cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt
      841 tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat
      901 tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata
      961 gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct gcatcaaaat
     1021 aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag
     1081 ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa
     1141 tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg
     1201 aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc
     1261 tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc
     1321 cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa
     1381 taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg
     1441 ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt
     1501 cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg
     1561 cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat
     1621 ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat
     1681 cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact
     1741 ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat
     1801 gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc
     1861 tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg
     1921 gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta
     1981 aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc
     2041 cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa
     2101 cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt
     2161 tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg
     2221 cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc
     2281 ttagattttc tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg
     2341 tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga
     2401 catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta
     2461 tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta
     2521 gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt
     2581 cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa
     2641 atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg
     2701 aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca
     2761 gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag
     2821 tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat
     2881 gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc
     2941 gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat
     3001 gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa
     3061 ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg
     3121 tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga
     3181 ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca
     3241 gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg
     3301 accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta
     3361 attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat
     3421 gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa
     3481 ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc
     3541 ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa
     3601 gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca
     3661 aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa
     3721 gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac
     3781 tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca
     3841 acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag
     3901 attgccaatg attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc
     3961 ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat
     4021 caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac
     4081 caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa
     4141 gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc
     4201 atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact
     4261 caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa
     4321 aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg
     4381 ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt
     4441 ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct
     4501 atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa
     4561 ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac
     4621 aagtcaaaat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt
     4681 ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg
     4741 cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga
     4801 atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga
     4861 tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa
     4921 gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca
     4981 ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc
     5041 ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag
     5101 tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt
     5161 cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac
     5221 catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga
     5281 aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat
     5341 tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta
     5401 cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg
     5461 cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg
     5521 aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg
     5581 cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc actggcactc
     5641 gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa
     5701 atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata
     5761 ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt
     5821 ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag
     5881 ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga
     5941 taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt
     6001 ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa
     6061 gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta
     6121 agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac
     6181 ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca
     6241 tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga
     6301 agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc
     6361 cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa
     6421 taccgtatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg
     6481 tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact
     6541 tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga
     6601 tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca
     6661 gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac
     6721 ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa
     6781 ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag
     6841 cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg
     6901 aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct
     6961 aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta
     7021 agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg
     7081 ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc
     7141 acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag
     7201 ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag
     7261 cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct
     7321 tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac
     7381 aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt
     7441 tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact
     7501 ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct
     7561 gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt
     7621 tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg
     7681 gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga
     7741 aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc
     7801 gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc
     7861 cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga
     7921 tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc
     7981 ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc
     8041 acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa
     8101 ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc
     8161 cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt
     8221 agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat
     8281 gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat
     8341 tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat
     8401 aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt
     8461 caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct
     8521 caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg
     8581 ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa
     8641 acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg
     8701 cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat
     8761 tggtctatta tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc
     8821 aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa
     8881 gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc
     8941 ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga
     9001 ctagatcctg cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt
     9061 tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt
     9121 gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa
     9181 tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc
     9241 ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg
     9301 gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat
     9361 ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat
     9421 gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt
     9481 tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga
     9541 gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca
     9601 taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca
     9661 ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag
     9721 cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct
     9781 ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca
     9841 ggaagtgacc cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg
     9901 atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca
     9961 cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa
    10021 tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg
    10081 cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct
    10141 ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta
    10201 agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc
    10261 tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc
    10321 gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg
    10381 atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact
    10441 ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa
    10501 tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa
    10561 gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt
    10621 tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt
    10681 cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt
    10741 acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt
    10801 ttcggataca aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg
    10861 ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg
    10921 gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt
    10981 gcttcttccg tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt
    11041 ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc
    11101 cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct
    11161 cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata
    11221 ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat
    11281 gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg
    11341 gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca
    11401 ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt tttttattaa
    11461 taaaacagca cctaacaata aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt
    11521 cgtttgcggg aatacggacc attttcctga ttgacctgga ttgcttggac cactcggctc
    11581 tttcggtttg tccgttgttt cttttacggt ctcgttataa acacgtggtg ttgcggattc
    11641 ataggcagag actggaactt tcccggattc taattcagcc atctcctgtc cgttaactac
    11701 tacatattgt ggttggcctt ttgcatccac ccaaaattca gggatgatga cctcgattct
    11761 cttacttgct tcatccatct cgtaaccttc cacacctttt aattcttcaa aataataggt
    11821 gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg aaatgaattt
    11881 gcttacattt ttgtcattca aaggatcatt gtctgctggt ttgatccatt gatttttcaa
    11941 gtcattagca ggagccatat ctaaataata tttcccccct tctatcatct gatatagagc
    12001 aaaaacagca ccttctaatg gttttccttt ttcagttgtt cctctttgac gtccatactt
    12061 gaagaagtat ggatctctta gataaccgac atttttagga tagatatgaa tctcttgcag
    12121 ttcctcggtt gattctgtcg ggtgataaat tggcaaaata gcagcaattg ggatagctgt
    12181 ctgatcgata tcaacatcca atccaacttc tggatcaatc gattcttcga aaatcaaata
    12241 aactgcatcc cgatcttggt ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa
    12301 tccgccaacc gtatctttcc cagttgttac cgaagtccct atttggacaa gcctttcttc
    12361 tgtgattagc tgacggatat tgtttctgtt cgtttcagcg atttgctgga cgaattcttt
    12421 aggagtcatg ttttcagcag cttttttttc atgataataa tcggtcatgt cgtaaatggc
    12481 aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata tctgctgttt cccctgcttc
    12541 ctcagagata gccaagccat cattttcatg gtattcaaat tgatcgttat aatcgatatc
    12601 tcgaatcatc cgtttatgca acacgaattc cacgacatct tcccctgatt cttctgccga
    12661 catcgcatgt ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa
    12721 acacaacagc tttttcatta ccttgctccc tcctttttag attgtctgac aaacaaacca
    12781 taggctgctc ctgcaagcgc catgaagaat gcagcaagca ggatatagaa taatcgtccg
    12841 gatcctcctg tagcaggcaa cggattttca ggatcgttcc cgacactaag ctgtaatctg
    12901 attcgatttg tctccaaaag ttcatattct aactcttgct gtagatcagg cgtctctgct
    12961 tcttcgtttg gataatacga aatctcaact tttagtgtac ccgcttcttt tcctggaact
    13021 acccgatcat tttctatgtc aggttcttct cgataactaa tttctaaaat aaagtaacct
    13081 ggaagttttc tatagctgtc aggtgacttc acttccttga tccgatagac cccttccaac
    13141 aagtctgaag cattgaatag ggaagaatcg tttggatctg gtgccaagcc tccctcgatg
    13201 atttcatact ctcctgcact attttgtttc tcaattgaaa attctgcgcc ttcaagacgt
    13261 cggctattca tactgtcaat tttcaaaagt tccaaatcga ttggcgctag ttcatcttct
    13321 aaggttactt gtagtacacc atcgatgatt tcatagcctt ccggcaaggg agcagtttcg
    13381 tcttcgatga ccgtatcttc aatgataaac ttaccttctt cattcagtgc aaactggaac
    13441 acttgatcag atagccgata cccatttggt gcgacagatt ccgagacctc atattcacca
    13501 atagacagat gcagaggttg gtcgtaattt aaatcgacat acttttctac tcctacggca
    13561 cgagaataga agtatccgat cccaggcaaa cgtttcgatt cttctggttc taacggatta
    13621 ccagtgtatg ctcgatacag cgggaaggaa gaatccccca ctttcctgat atcgaaacct
    13681 gcaccatcta atgtatggcc ttcatggtct aattttctaa ctcggatctc ccgataatca
    13741 aacgtattgt aagcagtgaa tgctacccat aaacgatcct cttcatctgt tgtatacgtg
    13801 agatattgcc aattttcttc acctgtttgc ttgagttgga tagtccctat agcattatcg
    13861 taaagtcgca agatccatgt gtcattttca tcaggttctt tgaaaccttc tgggttctgg
    13921 ctctcaatga ttttgtattc tgcatagttg gtagtttctt cactatcctt ataaacaaat
    13981 gaatctggct ctccgattgg atgaataact ttctcaccat ccacgattac atatttcccg
    14041 tcttcatcta ctgaatagaa cgtgagtctc cctttctctt cctctacttc gttcacacca
    14101 gaaagccctt tcgctactgg atatccgatt tccagtccgt taccatctgt ttcataaagt
    14161 tcaaactctg cgccattgat tggcatatta ctgccttcaa ttagtttatt gatattcatg
    14221 tggattggct tgaattcatt tcgcatttcc agtttgacat acaactcatc atactctgtg
    14281 ccttctatgt taggctcttg tacaacagaa agcttgtgat attctccttc ataataggaa
    14341 ttcggatctt taccttccgg tccgccgact ctcgaaccag atgtatattc atctacaagc
    14401 tcgtagtgac ctaacatctc atcttcgttg gctgcatatt ttttgatttg gaattgatac
    14461 gtctcatcta ctaactgata acgtggtggt ccagcgatct ctctgatcca aaatatgtcg
    14521 ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat cgctattccg
    14581 tccgtggtag ttttacttgt ttcgattggt tcggcttccc cactctctgg attttggtca
    14641 tataatgcaa attcgactcc agccaacggc tcgccatttt gacccttctt gctgacttgg
    14701 aaattacggc ctttatgttc gtaacgaatg gaagaagatg ggaaatacat ccgtccagta
    14761 gtgatatcat tctcataatc ctccaggttc tgaatatagg ccatattgtt cactgtgcaa
    14821 aaatttccgt cttgagctcc cgcatcaagt tttacttggt acttgatcct gaccatttcc
    14881 ccttcaccta aagaaatcgg agaaaattta taagcttggt tttcccgtcc gacatacgtt
    14941 gtcggagtcg ccactacttc tggtggggct tgtccatgac gttcttcatc ccatatttct
    15001 ggctcttggt acttcatgcc gttttctcct ggatctagat ggtaaaactg aatatctgcc
    15061 gatctgccag ctccaaatgg tgagacatta aaaccagttt catcataaag tgtggcttgc
    15121 tggatcgttc tcttgaaggt agaagagata tcctccaagg ctgtaacaat gtcattcttg
    15181 ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc aatatttttc
    15241 aatacatttc gtccaatagc atctagtcgt tgtctcccgc taacgtttcc atcgattcct
    15301 aaaccaatgg agaagatact tggcgggttc tcttgatact tgtttttcag ccaatgatga
    15361 taagcaactg tgttgatcgc agatgacttt tcttgcgaag taggactgcc attcgtattg
    15421 ttattagaag aaccatcacc aacccagtga gtcgctttat tatctccttc ttttacctct
    15481 ccataacgat agtgtacatt tacttcatct tctggacggt taaagtcaga tgcataggta
    15541 actggatgag taaaccctgc attagaagta ttcccagaaa actcctcaaa atttctgaac
    15601 aaaacgtcat tatcttcagc aatcttatct tccatcacag tccagttgct ccaagctcca
    15661 ttagtgctcg tacgataact tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct
    15721 cccacaacga ttaggatttt ttctggattt ctattttctc cgccattgtc tttatacaat
    15781 gtttcatagc catttttaag ccctagtgta agcggagttc ctccgattgg tgtacgattt
    15841 aatgctgtat tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc
    15901 gaactgattt gctcatggtt attgatagct gtactattat ggtaaaaatt caccataccg
    15961 attcgaatat ccagactcgg gtcagagagc agatctttac tcatttcgtt tactgcctca
    16021 atcaacgcag catttttagt ttgactattg gtcccagcgg ttaattcact catactcgct
    16081 gacttatcta gaacaaaaac gatatcgatt ggactgattt cctttttctc gcccccgatg
    16141 acatctagat agacattgta aagggttgtt gcatcttcag gatgtagatc agatgctgtg
    16201 ctgcttggca gaacaggttc tacccatttt ttgatgtacg ccccgttata atccaagtaa
    16261 ctgttttcaa aattccgttc atttccccaa agctgaacgg tttgagctgg aacgtttcta
    16321 tcttcttcgc ctgcagttga tccattatta gtagcatagt cgtaatttcg ccagttcgct
    16381 cccccgtttg tataatcact atcatcgatc aagttctctc cgtcatatgg cgagcctttg
    16441 tctccttgat agcgttcatt gctttgtgtc caatttccta acacttttgt atagtcattc
    16501 gttgtaaact tcgggtagcg atgctgttcg ttttcaactt ctccgctagg atagatatat
    16561 tcataggggt cttgcgtagc tgttggccca agacgctcca aaataccgtt gaataactgg
    16621 cgttgaatgc gctggagtgc ttgaccgctg ggtacgattg gttcgtccag cttttccatc
    16681 tcattttctt cgctacgttg aatttcagcg gaatcttctg tatcagtttc attttcttgt
    16741 aattcttggt tatctattct ttcatcaacc accacttgaa gaggtagtta cccttcgttc
    16801 acatcgggga tcttttctgt ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa
    16861 acttttccga cataccattc ttgaccatcg atcgtctcga cttttaggtc ttctccatct
    16921 tcatcatagg aggatagacc tgttccacga acgtttttta cggtaccaat cccggcggct
    16981 gccgcatcga ttgctaaacg tattctacct tcattggcac tttccttttt ttcaaaagcc
    17041 agatgccatt caagggtttc ttctttttca atatacttcc cttcaatttt tgcatagtcc
    17101 tcatctaaga atacctcatc ctcctttgtt tctgttgact cagcagctac atccagggtc
    17161 gtcatatgat agactgactg gaaatatggt ccaaatagca aactgacagt tactaactgc
    17221 aaaagcttca attttaaccc tttcacatcc ttatactcct ttcaaaaata tttgtcttct
    17281 ttcacaacaa tcaa
//
LOCUS       DACCXW010000055        16836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCXW010000055 DACCXW010000000
VERSION     DACCXW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.055"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..126
                     /locus_tag="IVY71_001555"
     CDS             <1..126
                     /locus_tag="IVY71_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7985612.1"
                     /translation="IYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            199..315
                     /locus_tag="IVY71_001556"
     CDS             199..315
                     /locus_tag="IVY71_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7985613.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(370..2052)
                     /gene="recN"
                     /locus_tag="IVY71_001557"
     CDS             complement(370..2052)
                     /gene="recN"
                     /locus_tag="IVY71_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7985614.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2070..2519)
                     /locus_tag="IVY71_001558"
     CDS             complement(2070..2519)
                     /locus_tag="IVY71_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7985615.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2626..3444)
                     /locus_tag="IVY71_001559"
     CDS             complement(2626..3444)
                     /locus_tag="IVY71_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7985616.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3453..4343)
                     /locus_tag="IVY71_001560"
     CDS             complement(3453..4343)
                     /locus_tag="IVY71_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7985617.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4345..4569)
                     /locus_tag="IVY71_001561"
     CDS             complement(4345..4569)
                     /locus_tag="IVY71_001561"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7985618.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4574..5911)
                     /locus_tag="IVY71_001562"
     CDS             complement(4574..5911)
                     /locus_tag="IVY71_001562"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7985619.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5912..6766)
                     /locus_tag="IVY71_001563"
     CDS             complement(5912..6766)
                     /locus_tag="IVY71_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7985620.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6863..7312)
                     /gene="nusB"
                     /locus_tag="IVY71_001564"
     CDS             complement(6863..7312)
                     /gene="nusB"
                     /locus_tag="IVY71_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7985621.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7305..7730)
                     /locus_tag="IVY71_001565"
     CDS             complement(7305..7730)
                     /locus_tag="IVY71_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7985622.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(7976..9040)
                     /locus_tag="IVY71_001566"
     CDS             complement(7976..9040)
                     /locus_tag="IVY71_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7985623.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(9212..9457)
                     /locus_tag="IVY71_001567"
     CDS             complement(9212..9457)
                     /locus_tag="IVY71_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7985624.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(9596..9970)
                     /locus_tag="IVY71_001568"
     CDS             complement(9596..9970)
                     /locus_tag="IVY71_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7985625.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10053..10967)
                     /locus_tag="IVY71_001569"
     CDS             complement(10053..10967)
                     /locus_tag="IVY71_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7985626.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(10988..11791)
                     /locus_tag="IVY71_001570"
     CDS             complement(10988..11791)
                     /locus_tag="IVY71_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7985627.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(11824..12813)
                     /gene="manX"
                     /locus_tag="IVY71_001571"
     CDS             complement(11824..12813)
                     /gene="manX"
                     /locus_tag="IVY71_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7985628.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(12916..13407)
                     /locus_tag="IVY71_001572"
     CDS             complement(12916..13407)
                     /locus_tag="IVY71_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7985629.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(13598..16432)
                     /locus_tag="IVY71_001573"
     CDS             complement(13598..16432)
                     /locus_tag="IVY71_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7985630.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
BASE COUNT         4726 a         3752 c         2795 g         5563 t
ORIGIN      
        1 atctatggaa tgaacattaa gttacctatt gctgaaaggg atgatgcctt tatatggcta
       61 gttctattaa cattatttat ttgtatcgta actacctatt atttaaaaaa agggaagttt
      121 ttataaaaaa gccctcttgt ggaaatggaa aaaagattgt acacttgaat caactaatta
      181 atgacgaggt tgaaaacaat gcgtgaaaaa aaacaaacaa gtacttttgc taaagtaaca
      241 aaatttgtca tttggacaat gctgatttta acgattggat ctgttgtatt gactgctgta
      301 atgagtgtca tgtaaaaata aaatatagct atcagctgct gaaaaggacc tgaaccactg
      361 cgaggtggct caggtccttt ttgcgagttt caatagttct tttgcatgtt cgagtgtgag
      421 ttttgtaatc tcacttccag ccagcatacg tgcaatttct tcttctcttt cttttgtttt
      481 caactctgaa acagaagttt gcgtacgtcc atcaatcact gctttcacaa tataatattg
      541 atagtcggcg actgcagcaa cttgaggcag atgagtgatg cacaaaactt gagagtattt
      601 cgatatttga gaaattttat ctgcgatggc ctgtgcaact cttccgctca cacctgtatc
      661 cacttcatca aaaataatac ttgtcacacc ttgctcagaa gaaaaaatag ttttcaaagc
      721 aagcaacatc cgagacagtt cgccaccaga agcgactttc accaaaggct tcagtggctc
      781 tcctggattg gtcgtaatgt aaaattcgat ttggtcaaat ccatcatgat tcaattgtct
      841 attttcttct tttaagaaac gtacctcaaa ctcagtgttt tccatataca gacttttcaa
      901 ttcatgcaga atggattttt ccaattcttt tgcgattttt ttacgcgctt catgaagatc
      961 tgcggcttgt tgctgaagta gaagctcttt ttgctctagt tcctctttca ttttgtccaa
     1021 ttgaccttct gaaaaatcag aagaagctag ctcttcggtg atttcgtcat agtaagccaa
     1081 tattgactca atggattcgc cgtatttacg tttcaactgt cggattaatt ctaaacgttg
     1141 cgtaacttct tctaaacgac tctcatctaa ctccaataaa tcaatctgac gactcatatc
     1201 gccgattgca tcttgcagca aatagtaagc actttggata ttttcataaa ttgcttcata
     1261 ctccacatct agatgggcga tcccttgcag ttctgaaacg gccaagccaa caccatctac
     1321 actgttttgt tcattattcg tcagtgctcc gtaacctgca gctaatccat cagcaatttt
     1381 ttgataattg ctaagttttt ctctttcttc ttttaatttc tcttcttctc cgacttgcaa
     1441 atcagcagca gctatttctt cttgctggaa atggagcata tctattctct gaacatagga
     1501 tttttcattt tgctgcactt ttcgtaccct tttttccagc tcacgataag agatatattc
     1561 ttgctgataa ctctttttct tctgttggaa agctacatct ccaaaacgat ccaataaagc
     1621 aagatgtgat tcaggctgga gcagttcctg atgttcgttt tgtccttgga tatctactaa
     1681 ataactgccg attcttctca gattagctaa tgtgataata tgaccattca ctcgacaaac
     1741 attttttcca gttaaggaca tatcccgtcg aacaatcaga ttgtcctcat ctgtttcgat
     1801 ccctaattca accatcagtt cagagaatcc ttcttgtttt ggtagttcaa acaacccttc
     1861 aagcacacat ttttctgctc cttgacgtat ataatcgctt gaacctcttc ctcctgctaa
     1921 cagacctaaa gcatcgataa taatggattt acccgcacca gtctctccgg tcagcgcggt
     1981 cattccttcg tgaaaagaaa gccgcaattc cggaataatc gcaaagttag taatactgat
     2041 ttcttgtagc atcacattcg ctcctcaact taaaggtaat gcaggaagtc tttcttcaat
     2101 aaaactgcct ggtcttcagt tcgtgtaatc atcaaaacat tatcgtcatc attgataatc
     2161 gaaaataatt ctttcttgta acgtttatca atcaaatttg ctgctgccga agcatttccc
     2221 ggcaatgttt tcaaaataac aaatttttcc atttggtcaa ctgccacgaa agcgtctttt
     2281 aatagttttt ccaatttagt atttacatct tcgtttgttt cagctggtat gctataacgg
     2341 tagccgccat ctgaagccgg taccttgatc agtttcagtt cctttatatc tctggaaatc
     2401 gtagcttgag taacagatat tcctttgctt ttcaggattt caacaaattc ttcctgcttt
     2461 cggatatctt gctcactcaa caatcgagta atcaaacgat gccgatcttt ttttctcatt
     2521 acgtcttcgc tccttagcat attcttctta atcaatgata gcttatttcc ttggtataat
     2581 aaagaagaat ccgcatactt ctcacttgat tttcacagtt acaatttatt tatggtcaaa
     2641 ctgttcgtgt gcatttgaaa ccacttctgc catattaata gctgaattga ttgttccgct
     2701 ttcgggtact ttcctcaagt gagccaagaa ctcgatattt ccttctcccc ccgtgatcgg
     2761 agaataactt aattccagca catcatatcc accttgtgcc gcaaatgaca aaatgtcttc
     2821 taaaactttt tgatgggtct gagggtcacg aacaattccg tttttcccta cagcttctct
     2881 gcctgcttcg aattggggtt tgattaaagc aatcacttct ccctcttctt ttaaaatagc
     2941 atgtaatgga ggcagcatca gttttagtga aataaaagaa acatcgataa cagcaacttc
     3001 tggaattcct aaggtaaagt cttcgggttt tgcataacga aaattcactc tttccattac
     3061 gacgactcta ggatcttgtc ttattttcca agctagctga ttgtagccaa cgtctaaagc
     3121 ataactcatt tttgcgccat tttgcaaagc tgcatcagta aatccgccag tggatgcacc
     3181 aatatctaac aatgtttttc cttcaacaga tagatcaaat gctttgagtg ctttttctaa
     3241 ttttaatccc cctcgagaaa cataaggaag ttttttacct ttgactctta attcactact
     3301 cactggaatt ttttctcccg gtttatcaaa ccgctcgttt ttttcgttgt aaatcaaacc
     3361 agccatcact gaacgtttgg cttgttctct tgtttcaaat aatccttgtt tgaccgctaa
     3421 tacatctacg cgttcttttt ccattctttc cctcatttct ccacatcaaa ttgcttgatc
     3481 atctggcgca tcagcccgcc atcaaaagaa aaaacgtcct cttctaattt gtctatgatc
     3541 ttgttggcgg ataacagctc aatctccaac gcctcacgtg tcttttctag gcctaataaa
     3601 cgcgggtaag tatttttttc catacgttca tctttaccag ctgttttccc taatgttttc
     3661 gttgtaccaa tcacatcgag tagatcatct cttatctgaa aagctaaacc taaatgttcg
     3721 gctaatcgtt gaagtagcaa taagatttct tcaggttgct tcgctaatat gccaccagcc
     3781 agcaaggcat aacgaatcaa atgccctgtc tttctttcat gaataaatgc taattcttct
     3841 aaagaaagct ttttcgattc gccctcgata tctgcagctt gcccagcaac cataccttgt
     3901 gatcccgcac aaacagctaa ttgctgaagc agcagaagct taggagaatt acctaagtgt
     3961 gccatactga ttagctggaa tgctcctgta agcaaaccat ctccggccaa tatagctaat
     4021 gcttcgccga atactttatg attggtaggt tttcctctac gaagatcatc attgtccatc
     4081 gcaggcaaat catcgtggat caaggaatac gtatgcacca tttctaaagc agctgctact
     4141 tgatagacgg gaacatcaat aggctcatca aaggaagcaa cggccgttaa aagtaataag
     4201 ggacgtattc ttttcccgcc tgctttcact gaataactca tcgcatcata gagtcctcta
     4261 tccgttgtat gctcatctaa aaaagacaag atttcttttt ctacagcagg tagatgaata
     4321 gctgaaaagt cagttaatct catctcagct ttcctcgttt ccgtcaaaat caacttcttc
     4381 atttgattct gtcatcattt tcgtcaaggt ctcttctgct ttagctaatg tatcttggca
     4441 atgtttgctt aattccattc ctcgtttaaa cgaatctaga gctgattcta atggaacgtc
     4501 tccttgttct aactgcatga caattttttc taattcttgc agtgactctt caaaagttgg
     4561 attttccata tttttattct cctttgtcta tctcttgtac ttgtccttta acgattccat
     4621 cttgataatg gatcgtcagc aaatcccctt gatgaatctc ggtgacactt tttactactt
     4681 gatcatttaa cgtcgtatag ctgtagcctc ttcccatgat tttcaatgga ctcaacaaat
     4741 caagagattg aacagcttga gcaaaacgct gctgctgttg tttcatatat tgttggattt
     4801 tctcttctaa gcgtttttct agataagcag tttcttggcg agctgcttta actcgataaa
     4861 gcggagccac ttgttccaat tggtggctta atgcgatcgt ttgtttttct ttcgcatgat
     4921 aaatttgctg tgtagcttga tataatcgct gtttcaactg gtctaatttg atggtctgac
     4981 cttcatacaa tctttcaggc tgccgaaata cataggaacg tcgagcacgc tcgaaccgtt
     5041 cttgtttgcg ctggatctgg cgtacaaacg cctgctgcaa tctagcttgc cgttcttgaa
     5101 tgcgcatcaa ctcttctgac aaaacaggaa cagctaattc tgcagctgct gttggagtgg
     5161 cagcccgaac atctgctact aaatcagcga tcgtggtatc tgtttcatgt cctacagatg
     5221 aaatcaccgg cgtattagct tgataaatcg cccgagcaac ccgctcttca ttaaacggcc
     5281 ataaatcctc aatcgaacca cccccacgac cgatgatcat tgtatcgaaa ttgcccatct
     5341 cttctactct ctggatattc ctgacaacat tgtccgctgc ttggtttcct tgtacaactg
     5401 ttgggaacaa aaccaattgg acaattggat atcttctttt caccgtcgtg atgatatcgc
     5461 ggataaccgc tccgcttgga ctggtaagga ccgcgattcg ctttgggaaa cgtggcagca
     5521 tttttttagg cccttgaaaa aggccttcct tcatcagctt ttcttttcgt tcttccaatt
     5581 cttgatataa agcacctatt ccatcaggct ccatgtgctc tacatagatt tgataagaac
     5641 ctcccgcttc atataatgaa atacgcccaa cgacaaggac tttcattcct tcttttggct
     5701 ggaatcgtaa tttttggaat gctcctttga acataatagc agatattttt gcatggtcat
     5761 cttttaagct gaaatattga tgggcattgg cacgtaaacg gaaattcgat atttcgcctg
     5821 ttaaatatac gcgttctaaa taaggatcgg catcaaattt tcttttcaaa tacttcgtta
     5881 atgtagtgac agttaaatat tcttgtgcca tctattcacc tgctccaaaa gcttcggcag
     5941 attttacggt ttgatacatc agcatcgtga tagtcattgg ccctacaccc tttggcacag
     6001 gagtgatcca gcctgcaata ggctccactt catcaaattt cacatcaccg atcagcttac
     6061 cgttagcatc acgattcatc cctacatcta tcactacagc acctggtttt acgaattctt
     6121 ttgtgacaaa atgtccacgt cctattgcta cgactaaaat atctgcttct tgtgccagtt
     6181 cagccagatt ttcggtttta gaatgagcaa cagtaacagt tgcatctgcc atcaagagta
     6241 gctgagccat cggtttaccg acgatattgc ttcgtccgat aactactgct cgtttacccg
     6301 ccggatcgat agaataagct tcaaacattt tcatgatgcc gtatggagta caaggaatct
     6361 tgtccggttt acccgcaagg agtctgccta aattcatcgg atgaaaaccg tctacgtctt
     6421 tttcagggcg gatagctaac aggatctttt cttcatctat atgctttggc aatggtaatt
     6481 ggaccaatat accatgaaaa ctaggatctt gattgtattt ttcaatttct gcaagtagtt
     6541 cgtcttcaga aatcgtctca ggataacgct ctaccaatga atggattccg atttcttttg
     6601 ctacacgttc tttatttctt acatagattt ggctggctgg attttcccct accaataaaa
     6661 cgactaatcc gggatggata ccattatctt tcaatttttg gattttttca gcaatctctg
     6721 cctgcatttt ttcagctaat tcttttccgt taatcaattc tgccatctaa ccactcccag
     6781 atattttttg tgttttcatt ctaccataga attttcccga tttataacag gtttatgatg
     6841 attcatagaa aaaagctagg acttacgccc cagcttctaa ttctttcaat acattggata
     6901 aaacaccgtt gacgaattta cgtgaacggt catcactaaa tgtttttgcc aactcgattg
     6961 cttcatttaa agcaacagcc gctggtacat ctgtcacata agtcatttca aaaatcgcga
     7021 tacgcaagat caccaaatcc atttttgaga tccgattgat gtgccaattg ttttttaaat
     7081 gtttttcaat cactttatcc aattcatctt tttttgcaca gacaccgcca actagcgtat
     7141 caagataaac aggaacaaac tctgattcat cttcgttgat catgtcgcgg tggtctaatt
     7201 cgattgcgtt gaaaatcgca tcttcttttg ttaggtctgc attgaagtct aatggaaata
     7261 aagcctgcaa tgccatttcc cgaatctcgt gtcttgataa ttctttattc attctcttcc
     7321 tcttcgtctt caaaaagttc ttcgattgtt ggttgttcta atttttcagg aacaacagct
     7381 actacatgga tattgacttc agataaatcg atatccgtca tgaaaaggac ttgctggctg
     7441 acccgttcct gcatttccat tgctacttta ggaactgaaa caccatattc tagataacag
     7501 taaagatcta ctttcaagcc atcttcctcg tttgctaagt aaacaccttt tccgtgagct
     7561 gcacgtccta ataattcagt gacattgctg gcaaaagttc cgcgcattcc gtatacgcct
     7621 tctactttag aagctgcaat cccgataatt acttcgatga cttcaggagc gatcacgatc
     7681 tctcctaaat cttggtttgc gtttaaaatc aaattttttt catctgccat ttatttattc
     7741 ctccctttgc ctaagcacga gatacatccg aattctctat gatgcggcat agtgatcacg
     7801 tggtaagaaa actgccgccg atcagctgca aactgctatg aattggagaa aacacccaac
     7861 tttattatct cttacagctt aaaagttctt ctatagtgta tcattaaatc gagtgatttt
     7921 ctataacaaa gtctgtgaac actgattttt acgcttactt cttctattga tgaaactata
     7981 agatgatcaa ttcttttggt gaacgagtca acacacgatt tccatcttct gtgatcaaca
     8041 agtcatcctc aatacgtact ccaccgattc ctggtaagta gatccctggt tcatcagtga
     8101 tcacatttcc tggtacaaat tgcttttctg cacgagaaga aacgtttggt ccttcgtgga
     8161 tctctaaacc gatcccatgt cctgtagaat gtccaaatgc ttccccgtaa ccatgttttg
     8221 aaatataatc tctagcaact gcatctaatt ggacaccagt cattcctggt tttgcagcat
     8281 caataactgc taattgagca tctaagacta tttgataaat ttcttttaat ttttccccag
     8341 gatcaccaat agcgaatgta cgtgtcatat cggaaacgta cccttcgtaa tagcaaccaa
     8401 agtccaatgt gatcaagtca ccttgttcga tgattttttt gcttgctaca ccatgcggca
     8461 tagccgaacg caatccgcta gcaacgatcg tatcaaaaga aacacctgaa gcgccaagag
     8521 aacgcatata aaagtctagt tgatttgcga cttcaatctc agtcatgccc ggttggatca
     8581 ttttcaggat gtgatcatat gccatatccg caatatgaca tgctttttcg ataatagcga
     8641 tttcttcttc atctttcact tctctgagtt cttcgatcat ctgtgaaaca ggtactaatt
     8701 ccgcatcgat gatttcttct aaaaccgaat attctaaaaa tgagactgtt gtttcctcaa
     8761 aagctaatga gtgcagttct tctttgcgta ctaagtttgc tacctcatca aaaataggtc
     8821 cgacattttt aataatttca aacccttgag cctgcgctgc cgcctgctcg gtataacgaa
     8881 aatcagtaat aaaaaatgct ttttccaaag taataacagc taatcctgtc gtacctgtaa
     8941 agttcgttaa gtaacgaaga ttgtacggac tggtaaccaa aaatgaatcc aaattttctt
     9001 cttgcatttt tttgcgcaat ttttctacac gtagcatcat aatgaaactc cctctttctt
     9061 tgcacgcttt tttccattat agcaaacttt tcttcgtaca cgacagttaa ataaaaattt
     9121 ccggaaaact tctttttatt ttaaataaat aaagccaaaa aggagtcaca gtctcgctgc
     9181 aactcctttt agtttactat tttgtaaaaa cttatgatcg ttttccaaag aaaaatgaga
     9241 caactgctac caaaatcact gcgcctaaaa tagatggaat aagtgccata cctgcagctt
     9301 ttggtcccca ttcgccgaaa atcgcttgtc caatacttga accgactagt ccagcaatga
     9361 tattcgttac acagcccatc gaatttcctt tactggtgat cgcacctgca attgctccga
     9421 tcacggctcc aacaatcaat gtccataacc aactcataat ataacctcct tctttgctaa
     9481 tcaattttag catatgggat ttaaagggga caaataattg gttttgctat ttcatatttt
     9541 cttctgcaaa gtaaaaaaat aaagaaaacc tcctcttaga agttttcttt attttttact
     9601 tgcgtttaaa catactacga ataccacgtt tcactacatc aaagaaactc aatgattgta
     9661 ccattctatc ttcatctaca tattctctga tcgcacgaag cactttttta ggttctttgg
     9721 aagaaaatgt gtatgtacca ttttttttcg tttgaatggc atatcgagga atccattttc
     9781 cctttagcat gactgatgct agtacataat ccacttcttc ccatggaatt tggatatatt
     9841 tgcgtctatc tcgcgcatgg taaaactcaa aaccattatt acctatcatg atttgcccgt
     9901 aatctgtcag acccgtaaac gaagtagcat caattaccaa gtctactttt gtattgattg
     9961 attgaaccat tttctccctc cagatatttg tttatgaaca gtatactata taaaaaaagc
    10021 tcgggacaac gcccgagcta tatttttatg gattataaaa gtccgatgac gtgtccaaca
    10081 acaccgacta cgaataaacc aaggatgata acgattggag aaaccttttt cttaagcaac
    10141 cacatacata acaatgttaa tcctaatgca gctaaaccag gaatcaattg gtctaagttg
    10201 tcttgtaatg ttgttacctt ttcaggagaa agggctaagc cattattaac ttgttcaaat
    10261 gcactcttga ttccttcacc acctgaaggc aatttatccc attcgatgta agcaccttta
    10321 tctaatttta cagttgatac aactggtaag aatttgattg atacccatct ttgtacaagg
    10381 gcagctaata cgaacatccc taaaattgaa gcaccttttg ttacgtcttg taacaatccg
    10441 ccagaaaggt catcagtaat tttagaacca gctttgtagc caaattcttg tgtataccac
    10501 atgaatgccc aacgaataat gttccaacct aagaagaaga tgattggtcc aaggatattt
    10561 cctcccatag ctaatgaagc acctagtgca cctagcattg gacgtactgt aaaccagaac
    10621 actgggtcac caacaccagc taaaggaccc atcataccta ctttaacccc ttgaatcgct
    10681 acatcatcaa caggcgcgcc attcgcacgt tcttcttcca gagccaatgt aacccctaaa
    10741 attggagaag caatgtatgg gtgtgtatta aagaattcca agtgacgttt taatgctgca
    10801 gaacgttctt cttttgtttt gtataatttt ttgattgctg gaatcataga aaatgcccag
    10861 ccaccgtttt gcatacgttc gtagttccat gaaccttgaa tgaaggtaga gcgccacgca
    10921 acagctaaac gatctttttt tgataatttg attttttctt ctgccatttt attttcctcc
    10981 ttcaagatta atagtcattc aaaatgtcgc ctaaagggtc accagagttg cttccgcctc
    11041 cattatttga tgaaccaccc attttagaaa ggttcaagta aataagagct aatgcaacac
    11101 ctaaagcacc tagagcaatc aatgttaatt gagaaattgc agctacaacg aaaccaataa
    11161 cgaagaatgg ccatacttct tttgtagcca tcatgttgat taccaaagcg taacctacag
    11221 ctacgaccat tcccccaccg atagccatac catctgttaa ccatgctggc attgattcaa
    11281 ggaatgattg cacagtttgt gctgggataa atagcaacgc tgctgctggg accgcgatac
    11341 gaaccccttg taaacataca gcaatgatgt gccacatttc aaccttacgg atgtttcctt
    11401 cttcagcagc cgcatccatc aaatgaacga ttggaacagc gattgtacgt acgatcattg
    11461 ttaagaacaa ccctgcaaca gccaaaggaa cggcaatcgc gatcgcagaa ggaacacctt
    11521 taacaccttg tccacctaaa actaaaataa ttgctgatgc tactgatgct aatgcagcat
    11581 ctggtgctac agcggcaccg atatttgccc aacctaaagc gatcatctga agcgtaccgc
    11641 caagtacgat accagcttcg aggttaccgg taactaaacc gatcaatgta catgccacta
    11701 gtggttgatg aaattgaaat tcatcaagaa taccttccat accagctaga aaggcaacta
    11761 atacgactaa aattattgaa ataatagaca tgataagcct cctataaatt ttaatattat
    11821 ttattacgct tttgctaatt cagcttttgc ttttttcaaa atatcatcca tgttatcgcg
    11881 tgaatcatta ggtactttac gtacatcgaa tttcacacct ttttgttcaa gcttctcaaa
    11941 cgcttcaacg tcttcttgtc ccatagacaa tactttactt acaacgactt taccgaccga
    12001 atgcgccatt gacccaacat ttaattcttt gatgtctacg ccaccttcaa cagctgtcaa
    12061 tacgtcttca ggattttcaa ataaaagcaa tgctttggta tttccaaaac gagggtcttt
    12121 tgctacttcg atcattttgc taataggaat aacatttgct tttacacctg gaggagcagc
    12181 ttgttcgatt agtctcttac gcagatcatc ctttgatact gcatctgaaa caacgataat
    12241 acggtttggt tgcactgctt ttgtccaagc tgtcgcaact tgtccatgta acagacgtga
    12301 gtcaacacgt gctaatacat atttgatttt tccgtctccg accacagttc cttctggaag
    12361 tgctccttta ggttgtgcgt cttcaactgc tgctttcggt gcttctgccg gttctaattc
    12421 ttccggtttt acttttacac cgtcttttgc tgtttcaatg atgtgtgctg caatttcatg
    12481 agcagattcc attgaaaagc gtgatgcgta ggcttcaatc aacattggta aattcaaacc
    12541 gctgacgatt gcccatttat ctttgtgctc ttcgaataat gtgtttgatt gattgaacgg
    12601 tgttccgccc cataaatcaa ctaagaataa cacttcgtct tgattatcaa aagaagcaac
    12661 cgcttcttcc aatttggctc tcaagtcatc tggaccttca cttggtttca aaatcacggc
    12721 tttgacgttt tcttgttcgc caaagatcat tgaaccggat tgtaagattc cttcagcaaa
    12781 ttgtccatga cttgctagga taatccctac catatttata cctcctattt acttttgttg
    12841 taacaaattc aaaaatatta ataattgcta accattacaa cagaattatt tgaccttttc
    12901 caacaaccat tcttgctaca cctttttgat tttcgctagt agttccatca aaaggatttt
    12961 cctgtcagca ggtacttttc gaatctctaa ctccacttct tgactgttca agtaacgaaa
    13021 ggaatcaata tcttgttgat ttactgaaac aaaattggta atcattgatt tgccttcagt
    13081 aaaactcata ccgccgatat tcaacgactt caactgtaca ccgcagcgga caaccgcttc
    13141 aacatcttgg ggattggtga ataaaagcat cacttttgct cgtgataata atccactatg
    13201 atagatttga atcattttct gcacggttat tacatttgct gtgatccccg gcggtgcagc
    13261 ttcttttagt agaaactttc tcaatttatc ttgagcgact acatcgctga tcacaatgat
    13321 acgttcaaca cctgtttgct tcgaccaaac tgttgcgact tgcccgtgga ttaaacgatc
    13381 atctatacgt gccaatcgaa tatccatctg caaaactcct tcttcctcac tacttcacag
    13441 aaacccctat catttctgtg tgaacaacca cctcctagca cacactacta taagcaaaaa
    13501 gcgtgccaac ttttttacga tacactaatt gaatttaaag gattcttaac caatacacaa
    13561 aaacgataca ctataaaagt gtatcgtttt tgtgtattca tggcacagtg tattcttttt
    13621 tatctaactg attatggact agacgtagca gataatacat ttcttctatc ggcactttta
    13681 cttttatctg atcttccaac agaacaactg aggctgccaa ttggctataa agaggatcat
    13741 tcgttgtatt tgtcaattcg ttttcttcca cagtcaatgg ttcattccga ataatacgtt
    13801 cgatcattcc tgctgtgtgc atcactaggt tgattttgaa tccatattct tcatcgccga
    13861 ttcccgtcgt ctgagagatt tgtgtagaaa attgccacat tggatcgatt aacttactac
    13921 cattaataaa gatgaagtta tcagagataa actcaacaca cgtattttta gctgactctt
    13981 catctaggga gatgaattca gtttcctcca attctgattc catcagcaac tgatccaaaa
    14041 tgacttcgat attttgatca ataaaacgtt ctaaaggaat aaatggggct tggatattca
    14101 aatcagtaat cccagtagta gctatgatct gatattcttt ttggataact ggcagttctt
    14161 ctttcacttc cacaatcgac aaagctagaa cttctaattc tgtttcttga cgcttgttta
    14221 ccgctcgttc gatcaattct ttgattcttt gtgctgtacc ttcgccagat gcgcagatag
    14281 ccaaaacagc ttttttctta tgaataggtt ttgcttcctg aacagattct acagaagata
    14341 tttctgcgta acctcgaaaa tttttcaatg attcatgcaa catatctaga tctgtcccta
    14401 atactgagac ctttcgtaca gtttccaaaa ctagagaagt tgttaccata tcaacagtac
    14461 gtactggaat tccagtgtct ctttgaattt gcgaattaaa cgacgcaagg gaccccatat
    14521 ctactaagag tatgaccccg cttccttcat ctacttcttg tacattgcgt tcaattcggt
    14581 ccaatgctgt tttcggatcc atatccaaag gcatgtctac tgctcgtacg ttctctgcat
    14641 ctaacaactg ttggacaact tggaccatac tgcttgctgt gctgtttccg tgagctgcaa
    14701 cgactacacc gattctgccg cttgcttgat ttgcccgtaa cgagacgagt agaacggtca
    14761 agtaatagtc ttcactttct ggaatctcca cttgaaaata cgtttcgata taactacgca
    14821 cttctttcgc aacagcatat tcatctggaa aatcaaaagc catctcacgg atgttatcat
    14881 tcgtatgccg ttcttctcct aagttgattt ttttcaaaaa agaactgata tgcaagctca
    14941 tagcataaat aaagttttgc tgaaactcat atggtaaaga tcttttgacc attacataaa
    15001 tctgattggt cacttctatg attttcggat ctacaaattc tgctaatttg ctgtctgcat
    15061 taaaagaaaa accatggtct ttatagaaag acttcagatg gatgttgata tctgtagaaa
    15121 tgaaatgatt gattgcttct tgatccaaac catccgactt cagtaaagct gctttatctc
    15181 cgatgatatc atatagatta taaggtaact cataggagtc cgattggatt ttcatgatcg
    15241 tatcatttgg tgctacagtt atctttggct ccaatatttt agaaagctct gccattgcac
    15301 tgcggttgct tgctaattga atcaatccgc tacgaatccc ttctgtcaaa tcatcgattg
    15361 tgatcgagat ttcgggtgat tgcatatgat ttagaaatcc tcgtgcacat actaactgga
    15421 tatttgactt caattgtcct atatttccat aggtcacact gccgatcaat gcttttgcta
    15481 catcttctgt caaagaaatc tttcgctgcg tgcggtttgc ttcgtgggca atcatgattt
    15541 ttactaaatc gactttctca tttgcaggac gtttttcaaa agccggaagt ttgatattga
    15601 ttgggatacg ccgaacaaaa gtttctaata gagaagaacc tgggtcttct gtggtcgcac
    15661 ctacgatccg tacatttgct tcatgggatt tcgttgtttc cccaagacga ctgtaagttc
    15721 catgatccat aaagtaaaag atcatttctt gaccttcagg cggcaaccgg tgaatctcat
    15781 ccaaaaacag cataccgcca tccgcttgat cgataatccc cattttttct tcatctgcgc
    15841 ctgtaaaagc accttttgcg tacccaaata aatgactcat caaaagctca gggttattgg
    15901 cataatcagc acaattgaaa acaatcagtt ccgtttcctc atctattaca tgatgcgctt
    15961 ttgcaaaatg atacatcgcg tgtgcaaaat aagtctttcc tgatccggta ggtcctgtaa
    16021 tcaaacaatt taatcctctt ggcggataca aaatcgcagc ttttgcctgt tccactgaat
    16081 ttttcatgct gccgttagct ccaatgattt ttgcaaagat atccttagta tctatttgga
    16141 gtgtattttt ttcttcagag accggcgttt ctttgtaact gactacatgt tttgtaactg
    16201 gctttacgtg cgtaacgagt gtttgatata tgtaacgtac tggtcgtccg tctgtcttta
    16261 tcagttttcc ttcgcgcact agctgattca gatctttact gctatttgtt cgctgaatcc
    16321 ctaacacttc agcaatttcc tgtgttgtta ctccttgatc atattctgcc tttgttaaat
    16381 gagcggtttt ttctttgaca tacatataaa ttcggtcaat gcgttttgcc aatggtatcg
    16441 ctccttttct atagactatt gtacttaggc ttcaaaacat aatactatgt atttatttct
    16501 gtttcgtcaa ctatttacct gattttgttt tgttgataac acgcggatag atagaaatgt
    16561 tcaatttaaa agaacgaaaa atcccgatac gatttttata atggaaacaa gaaaaagaac
    16621 actgtttacc atataattat ttcactgata gcaaacaata tagcttccat caattgaaaa
    16681 cgatagttat caaaacatcc tgattccaaa ttggcaaaaa aatattaagt gtaaaaagac
    16741 aaagtaaact tcgtagcctc cactacaact tttattttta taaacaaaaa aggctgtgac
    16801 aaaagtcttg tcacaacctc tttaatcata agattc
//
LOCUS       DACCXW010000056        16620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCXW010000056 DACCXW010000000
VERSION     DACCXW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.056"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2131)
                     /locus_tag="IVY71_001574"
     CDS             complement(<1..2131)
                     /locus_tag="IVY71_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985631.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWM"
     gene            complement(2128..2832)
                     /locus_tag="IVY71_001575"
     CDS             complement(2128..2832)
                     /locus_tag="IVY71_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7985632.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2844..5153)
                     /locus_tag="IVY71_001576"
     CDS             complement(2844..5153)
                     /locus_tag="IVY71_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7985633.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5348..5812)
                     /locus_tag="IVY71_001577"
     CDS             complement(5348..5812)
                     /locus_tag="IVY71_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985634.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5812..6438)
                     /locus_tag="IVY71_001578"
     CDS             complement(5812..6438)
                     /locus_tag="IVY71_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7985635.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6445..6810)
                     /locus_tag="IVY71_001579"
     CDS             complement(6445..6810)
                     /locus_tag="IVY71_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP7985636.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6800..7138)
                     /locus_tag="IVY71_001580"
     CDS             complement(6800..7138)
                     /locus_tag="IVY71_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985637.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7128..7454)
                     /locus_tag="IVY71_001581"
     CDS             complement(7128..7454)
                     /locus_tag="IVY71_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7985638.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7435..7713)
                     /locus_tag="IVY71_001582"
     CDS             complement(7435..7713)
                     /locus_tag="IVY71_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP7985639.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7715..9079)
                     /locus_tag="IVY71_001583"
     CDS             complement(7715..9079)
                     /locus_tag="IVY71_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7985640.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9092..9667)
                     /locus_tag="IVY71_001584"
     CDS             complement(9092..9667)
                     /locus_tag="IVY71_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP7985641.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9633..10820)
                     /locus_tag="IVY71_001585"
     CDS             complement(9633..10820)
                     /locus_tag="IVY71_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7985642.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10884..12611)
                     /locus_tag="IVY71_001586"
     CDS             complement(10884..12611)
                     /locus_tag="IVY71_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7985643.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12608..13060)
                     /locus_tag="IVY71_001587"
     CDS             complement(12608..13060)
                     /locus_tag="IVY71_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP7985644.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(13172..13552)
                     /locus_tag="IVY71_001588"
     CDS             complement(13172..13552)
                     /locus_tag="IVY71_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7985645.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13549..13935)
                     /locus_tag="IVY71_001589"
     CDS             complement(13549..13935)
                     /locus_tag="IVY71_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985646.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13937..14215)
                     /locus_tag="IVY71_001590"
     CDS             complement(13937..14215)
                     /locus_tag="IVY71_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985647.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            14263..14991
                     /locus_tag="IVY71_001591"
     CDS             14263..14991
                     /locus_tag="IVY71_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP7985648.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(15137..15613)
                     /locus_tag="IVY71_001592"
     CDS             complement(15137..15613)
                     /locus_tag="IVY71_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP7985649.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15573..15920)
                     /locus_tag="IVY71_001593"
     CDS             complement(15573..15920)
                     /locus_tag="IVY71_001593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985650.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15907..16092)
                     /locus_tag="IVY71_001594"
     CDS             complement(15907..16092)
                     /locus_tag="IVY71_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985651.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(16093..16365)
                     /locus_tag="IVY71_001595"
     CDS             complement(16093..16365)
                     /locus_tag="IVY71_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP7985652.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16362..16565)
                     /locus_tag="IVY71_001596"
     CDS             complement(16362..16565)
                     /locus_tag="IVY71_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985653.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
BASE COUNT         4661 a         3522 c         2582 g         5855 t
ORIGIN      
        1 ccatccattg tccatttgta aatgtccaaa taagcaatcg aacttgtgca tctaagctcg
       61 ttattggttc tttaatacct gcagcattaa ataagttttg aacataaact tttccatccc
      121 atgttgctgg accaacaaga ggatatgaag aaccatccca ctgaactaat ccgtatgctg
      181 gtccacctat ttgaacagta tcaggatcaa aagttcctcc tgtttcttgt tgaatgttcc
      241 ccaatattcc acatgcagat tgtttcgtaa atccgttatt acacaaaata tcgtatattc
      301 tccaagctct cttttctgca tctgttttta attcatcagg atacccacca ggtgtttctc
      361 ctccgccaga tggaccgcca ttttgtcctg gtatcacttc tttacctcta acagtaagtt
      421 ttccctgaat atctaaatca ccgaaataat atgcttttcc gtttcctaat aacacaaatc
      481 ctttttctgc agttggagaa attaaaatgt attttccatc gccatttgta cgtattacca
      541 aagaattttc ctcaagaggt gtaggtgtta cagcatttgg aaatggattt cctgctgaat
      601 ctgttgttcc gatcgttcca atggattctt ttctattcca gaattccata ccttttttgg
      661 ttagctccat gatttttttg ttttcattcc aaatttgaag cgtgcctttt acaagtttta
      721 acacatcacc ataagcatta aaggaagttt cgaatacttc tgcatttatt gttcctactt
      781 tgatgaagtc agcaacaatt tctccttttg atgtgatagc aataccgaac gggccattta
      841 ctccgttgtc tgaataacct aagccattta aattccaacg ccacactcgc tttgcattcg
      901 ccacatttgg agtatccatg ataagaatct ctgatggagc tttttctgga cgaaaaacga
      961 catgcccacc agaattacca gtgatccatg ccgtcgcatt caacacattt tgaactaacg
     1021 tttctgttcg attgtcgatt ttctttttca attcttgtgc ttgagtattt actgccgaag
     1081 tgtacagtga taaatcatta cctaaaacaa tatccttata tttcccaagc gttgggaaat
     1141 aagtgtattc gaccatacgc tcttttatct cgatatctag ctctttcgcc ctaacatgag
     1201 cgacatcacc aaagtgtaaa gacgctagct cttgatacat gtcaccgtat tcgagtgtgt
     1261 gttccaacgc caccatactg actgtatgag tagcttttgg ctcatggata cgatcattgt
     1321 caaataaggt tttgccccat ttgatcaatt catcaactgt tttacaatct ccgttttcac
     1381 gtttggcaat acgtcgattc tcgttggtta ctccatcaat ttctaaataa ccatattcga
     1441 taggttcttt gtcttcatca taatcgttat caggaacacc gccaaccaaa tacaaactat
     1501 tgacaattga ttcttcatca atttcttctt cgattgcttc taaattgatg ccaaaatcaa
     1561 tccgaaaacc attatctgat ccaatctgtt ttaccagttt caaatcatag ttatccatat
     1621 caagttccgc gctagtcacg ccagtcaaat tttgattacc gttatttgaa ccaatgattg
     1681 cttcaactgg cgcgacttgt cttgctgtaa attggtgcgt tgtccctaca ttcgaaagat
     1741 agttaaattt ttgatcaaat gccaaactgt ttttaagatt ggtcatgatc tgagacccat
     1801 tgccgttatc cgtaaacgac ttgacaataa agttcttatt ggccataaaa ccaatatgcc
     1861 gagctgttac agaaacggag gtcaaattct ttttgacatt ataaatttca aaatattgcc
     1921 atgatccatc tggtaccttc gcttttataa aatttccttt ttttaaataa gagcgatact
     1981 gtccgcttct tgaatagtta ccataaaaac gatattggcc attcagcaca cgattgattt
     2041 ctggcaaatc ttcccaatca actaacgacg ctccgttcac gctcaaatca tctggcattt
     2101 ttttataagc ataaataaat tcttgcgtca cagatacaca ctcctattcc aaaaccgcac
     2161 ttctttaaat tttccggata tttttacctg attccattca ggctgcaata ctggccaatc
     2221 accacgcgta aataaattca aaccttcttg tattgctttt ccaagctgag tatcaaccac
     2281 gattgttgca gctaacgtat taagtattgt taggctttta tcaccgacag cgatcgttat
     2341 atctcctccg ttcgattcta tttccagata gggatgtgca atttcgtcac catgatcaaa
     2401 aatatccata acactagaat gaaaaatttt aggtgcttca ccgatttttc ttttgagtgg
     2461 ctgacaacga aaagtaacat cgaatgtata aaagaagccc cactcgtttt cgaaagggac
     2521 ttctttatcc atgctgcaga tagcctctag atacttgtct ggatcattat gtgtgattaa
     2581 ttggctttta ccagtgagcc atcgtttgac ttctctcagt ttcgaatgtg gaatagtgat
     2641 cccttctatt tccaaatcaa aaggttcata gtcatcaaac gtctccgtca attctccgct
     2701 tcggccctga atcgtgtaag tttcgtatcg cttgttcggc ataatatctg gaagctctgc
     2761 ctcaatgatg caattcatat caattactgc attccgattt ttccaaataa aatttggttc
     2821 atctggattc ataaattgtc gactcaaata ggaacacctc ccaaatcacg aatagcctgt
     2881 ttgtttgctt tagcaaattt acgattcata cgatctaact cagatggatt gtttgcatcg
     2941 actttgccaa tgtgtatatg ctgttcaatg ctacctccag aaactcttcc actgatccct
     3001 ttgcgttttt cttcgtctga taatggagta actgtggttt taccattctt ggcagtcagt
     3061 aactcaggtc cagcttcacc aacaatcgct tgtccattga ttagatgacc gccttgtgct
     3121 aaataaggca gttttgaaat agaaaatgat tttccaccta caccaggaac ccattttggt
     3181 atctttatat tattcaatcc accaataaat ccattgatca atccaatcat ggcattaata
     3241 ggagctttcc ctacagcaac aataccatcg aaaatacctc caaaaatatc aactagacct
     3301 tgccatgctt ttgaccaatt ccctgtaaat actccagtga taaaatctag aaaaccactg
     3361 aaaattcgtt ttccagcatt ataaaaatta ttgaaatttg ctataacacc atcgaaaaat
     3421 ccaccaaaat aaccacttaa aaaattaaat atttcaacag ctacatctga aacacctttg
     3481 aagaaagaat tcactccatc atgaaaccat ttaacgttat tatatgccca aattagtcca
     3541 gtaactaatg cgccaatagc tataactgct aatgcaaacc agcctcccga cattccaaat
     3601 aaaccagcaa gtccttgcca aactcctata aaacttttaa cttccccaac gatttttgta
     3661 acagatccca taagtgaacc aagaacgatt agtactggtc caactgctgc cgctatacca
     3721 gcaattgtta tgatccaatt ctttgtatct tgatctagag acccccacca tttagagaat
     3781 tcctgtaata aggaagttgc tttttcaaaa aaaggcaaaa ggcttacttg gactgcttca
     3841 cctacgtcag ccattgctaa ttttgcattg ttcatagctt gatcggcttg atcaattggg
     3901 tcgagagtag catcgaatgt gtctcctaca gctcccccac tttctccagc agtttttgct
     3961 aaatcttcca ggttcaaagt acctctacga attgcatcgg ccattctagg accgccttta
     4021 gtcccgaata cttctgctgc tgcattgatg gcttctgttt cagaacctgc attttttacc
     4081 ttgtcttgta gttcttctag tccttgactt aaagattttc cgtctttcgc ataagccact
     4141 gttgctttag ataaactact taaagctgcg cttgaatcaa caccagattg ctcaaattgt
     4201 ccaagtaaag tgaccccttc accaaagctc aaccctaatt gtttaatttg tggtgcgcca
     4261 tcaattgctt tttgcatcaa gtcgtctaca gattgaccag tattctgcga tgttttcgtt
     4321 gttacatcaa ggacagaatt caaatcatca tattctagtc cataagcatc aatagcttgt
     4381 cgagcagata ttgctgattg cgaaacatct gtatcattga tttcagcgta ctgtaggaga
     4441 taattggttg aatcttccag ttttttatcc atgaatccaa attgtgtatt tacctcacca
     4501 atagcttccc caaccgtttg taaaggtaaa tgagtatttg aaccaacgtt tttgaaagac
     4561 tgtgaaagtc tatcagcttg atcacctgtt gctccggttt ttgtaatgat ggtatcaagt
     4621 gcctcgtcaa cttcaccaaa tgctgcaagt cctgctgctc ctgccgctac aataggcgcg
     4681 gttactccaa cagacatttt ttcaccaacg ccttttactt tctcacctgt ttcttcgatt
     4741 ttctggagtt tttttgctgt atcaacagaa acgtcacctt gttctttaag tgcatcgttc
     4801 gtctgttcta atgctgtacg aagtttattt tctccggttt cagaattgag taattgttta
     4861 taaagttttt ctgattcagc agaatattca ccggtttctt tgacagattt ttcatattcc
     4921 tctctcagca tttgagatcg ttgttcagcc aatcctaatt gtttttctaa tttttgttta
     4981 gccgctcgca atttttctgt ttgagttgca tccttgtcca tcgcagacac ttggtttttg
     5041 tactcggcag cggctaagtt catttctttg ttgatttctt tgattgtttt cgaataactg
     5101 acttcgccat tcgttttaaa attaagaacg acatcagatt ctttaccagc catttatctt
     5161 agcgctcctt tctaccacca aggcgattta tccatagtta cagattgagg tggttcaaaa
     5221 tcggtgtttt gttgtaacca ctgtaaataa gatttgagcc acaagttagg tgtagacttt
     5281 aaaaagaaac tctcactcca attcaataga gtgagagcaa cgtaaatata aaaactccaa
     5341 ggagttccta tctcttccga ttctttttgt ttttctttct ttttgcttgc gctttttgaa
     5401 agtcttgtgg cttcttggat ttttttaggt cttccacctg aaatgtctgg ctagcaaaaa
     5461 tttccataca ggtactataa gcagacaaca cctctccact cattcccaaa aatttgaaaa
     5521 tagtttctgg atcttcctct aaaccaccag tacgcagcat ggcataaatc aaggcacgca
     5581 tgatttttag atcactaggc gataagttag cagaagaaat ttgtccttct tttttagata
     5641 gcatggcgtt catatcagat tcaaattgcg aataatctcc accatacaca tctgctataa
     5701 attccatcgt ttgcatagta aatgaaatag ggaattctgc gccttgaata attacaacag
     5761 cagaattttt taagtcttca acgtgaattc cataatcaga taaccgagcc attaacccgc
     5821 acctccaact ttagccaatg ttttccactg ttcttcatcg tatacaggtt gagcaatgaa
     5881 tttattaaat aattctagag aagctccgtc tctatttgaa tcaaaacttg catacatcac
     5941 attattgtat ttcagtccga tggaaaccag atttgcagtt acatcgtcaa tttttgtttc
     6001 ttcctctgca gtggcatact cttcgtcaat cacattggat agttgtgtat taggatacca
     6061 aactgctttt ttaccgccat tttcaatatt tccaataaat ccaaaggcga agtacggaaa
     6121 ttctcgtgcg gtatttttgc taaacgttac tccgctttta gcaactaatc ctttgatttc
     6181 atccatcact tctatcggaa tacccacgtg atccaacgca agttcatgtt ttgtttctcg
     6241 cgacacacga cgaaacattt tgctggatgc ccatttttct aatgctgttc cattcccttt
     6301 gacaccgatt tttgtggcaa ttggcaatcg taaaacttca ctataagtcg gtgcagttcc
     6361 aacagaatct ggcgttgcca tcatcgcaat taaaatgtcg tccaatcctt caaaataaaa
     6421 agtatcttgt tttcccaatt aaaatcaccc ttcccataaa tcaagtattt ttctagtcat
     6481 aatttcttca attttttctt tgttttgttc atacgtacca cttgcaaaat gttgggcacg
     6541 ttgatttgtt gtgccatttt cggcaaagcg ccagtaaaag gccgtctctt caaattgaac
     6601 tttcacttga tcgttttcaa ctactacttt tatttgatcg cgcatatgtt tcttttttaa
     6661 gagcgactta ggaatattag gaagcaactg ctttagataa aagttagctg cttcttctaa
     6721 agattccaac gataattttt tcggatcaac ttttgaaagg tttcctaaat aatccgatat
     6781 ttcagaaaaa ccattcttat tacttggcat tttccacaca cctcacatat gtgtaatagt
     6841 tggttacggt atcgtcgttc tcatcacctt gtatacctgt aaaatctgaa tatggaatgc
     6901 cagcattttg cagcgcttgt tcaataacaa ctaaatcctg ttctgttcca agtgtaaaaa
     6961 aagagacttg gtaataaggt aattttttat agactttacc ggatgccatt tttttgctgt
     7021 tactcacatt tgagtacaca atgtatggat acatcgtccc taatctggct ttgtctctga
     7081 acactgctaa ctttgttgat ttcagcgctg ttttcaattc atcaaagcta atcgacataa
     7141 gctaaactca actccatttc tcttgcatcg ggattcgtat aaatgcgagt aatgttatac
     7201 gttacagaat caattttgag cgcacttaat ttctctgtga tggatttatc ccatctgact
     7261 ttaattcgtc tgacaacgtc tgtcttggct tgctgtgata aatatttttc ttgagaagtc
     7321 acaccgagtt cttcatagaa tattagacgc ttaaattcgt aaattgtagt tggacgatcg
     7381 tttccgtctg ttcctgtttt gatgtctagc aattcggctt tccatctgag attattagtc
     7441 tgtctcttcg gcattttgaa tcgctccttg cacgataaat ggcgtcatgg cattcatagc
     7501 tttgtcgagt tcatcctctg aaactctata ttcataggca atgccggcaa ccatcaaaat
     7561 aagatattct tgttggccac cagttgctgt tttgacataa tcttttgcca tatttaaata
     7621 aaaagagagc aaagaatcat ccatgccctc ttcaaaatga atatgtgatt tgaatttttc
     7681 ctctaaagac aattctttag tttgctcttc catcttaacc accaactggt tttgtaattt
     7741 cgtagcgata tactgccggt tcaaatggag aataaaccaa ttgaccatct agcaagttgt
     7801 aaatttggaa tccaatttga tttttaccag agaatttttc aacaagtttt tgaatttcca
     7861 aggcaccaat aacttcttga attttaaatg cagaaaaatc gccaaaatat aaaactggcg
     7921 tgtctggttc acccttttta tctgctgcat ctgtccaatc cacaggatag ccaactaatt
     7981 ggtaaccaat tccaccttct gcttgtgtga atggccgcaa caaaggaaat ccatcatctg
     8041 ttttcatttt ttcaatggca gtcaaagcag ctcgattaat aataaagcgt ccctttttca
     8101 tcacttctgt cactggtgta tttttaaatt cgattaatgc atcatataat ttttgcccag
     8161 cacctgaagc agttagatct aaaggttttt taaatgctac agcctttttg gctaatgcac
     8221 caggattttc atttccagcg tcatcaccat tgaacatata attgatttct ttacgcacat
     8281 aagctttttt caattcttcc acaacaatat cttcaactgg aacaccagac atttttaata
     8341 attttttagt tactgttgcc aaagcatcga attcggcagg attaagcaaa atttcatcaa
     8401 actgaatagc tgtttcagca atatcagttg aacgctcttt cttgtttaca ttcgcatctg
     8461 ctttcttcac aagaattgga tatttgacat ctcctgatgt tcgcaccact gttccgtatt
     8521 tacgaagtaa attttcttct tgagcataag taataacttc agatgcaatt acttctggga
     8581 cagtaactga accgttgcca gcttcaatcc ctaaagctcg agcttctgct tcagaaatat
     8641 ttccagctac aaaattagca aatgcttttc gtagttgttg atctttttgt ttcttggtca
     8701 tttttgcacg tgcctccaat ccgttcttaa ttgatccaag taatccatct cgttgctgtt
     8761 gagtaatcat tccagaacga ttttctgaac tatcttcact gtctgaatct tcttcttgat
     8821 cgttgtcttc accagaacgg ctttcgtctg aatccgtact attcgattga tcatctgtat
     8881 tgtcgtctgt ttcatcagtt ccagaatctg cgcctaattc gtctttaatt ccgttcaatt
     8941 catcaataac actgtcaatt tcttctttca cggcttctaa atctgcttcg cgtaattctc
     9001 ctgattcaac tttttcacgt aattcagtca atcgttgctc acgacgtgcc ttcatttttt
     9061 tcaataattc tttatccatg tatttttcct cctacgcttc tagcgcttga ttaatttttt
     9121 tgattaattt ttttctttct tcgacgtttt tttctaattc atcacgactt cttaatgcag
     9181 cttctgtatc ttcgtatgct ggcaaaggca caatagaaac ctcgtataat tccacttcat
     9241 ggattgttcg aagcattggt tcagaattgt agtcccacgt ttcttcagtc ggcacaaaac
     9301 caaaactaca ttggttgatg tctcctcgtt ccatagattt aactaagtcc cttgctaccg
     9361 ttgtgtccgg taaatcaact tcgaatttca aaccacgttc atcttcttca aggcgaaggg
     9421 ttccgctttt agtacgtcct agcacattgg accaatcgtg attgaacaaa caacgaacat
     9481 ccgaattact aattgccttt gagaatgcac caggaacaat cacttcgctt aagtcatccc
     9541 ataaaagtgt ttgactgttg aatacagcag cgtagccgct aatggtcctc gtatgagttt
     9601 cttcatcgga acgtgttgaa agattggtga tgtcaatcgt gcgaatttcc ttcttcttca
     9661 tttccatcac ctcctttcaa gtcttgatca ttcgttggta aggaatcatc tgttgcattt
     9721 ttctggccaa tcctagataa gtcatttgaa atatagatag cttgtgtttc tggagtattc
     9781 tgtttaggaa aaccaagcat ttctgccacg ttatctggac ttgtaatccc ggtacgaacg
     9841 atgttgtagc caatatttgt ttttgttgag taaggaacaa aatccaaaat attaattttc
     9901 cattccactc tatagccaga attaggcata aaaaaaagag cggtgtaatg ttcgctcttg
     9961 tttttcaata ttggtttgat tgctttgttg tgcagataca tcatcgcttt ttcaatatct
    10021 gtcttcatca gcgattgata cgtatcaaca ttgattccta gaaattttcc taagtctttt
    10081 ttgtaaacac ctaaataatt caaaattgcc gcgtcatcaa caggactttt taatgtctcg
    10141 atggaatatc cttttcccaa aggaatcatc ttaacagaat gatcactctc attttgcgtt
    10201 ccttccagtt gatccaatat agctttgaca atttttgttt gggcgctatt atttggattg
    10261 atgtgggcgt ctagtttaag catgaacgca agtaaaccgc ctttagtata tttgtccgtc
    10321 aaaacttttt cagcgctcag aacgccttct agagtgtttc ttgcaagatc aattattcca
    10381 gcacctttta atgaatcagt tccgatgttc ttaatgtgtc gaatcatttg accaggtatt
    10441 ttttgaccat tcatttcaaa ttcttcttga agtcgttcat tgattttagt tgttacaccg
    10501 tacgccaaat gaagctgatc cctatccgtt aatgggaatg tctcaccatt gatcaataaa
    10561 gtatttgttt ccaatttggt aaattcgaat cctgtcaaat aatcattggg actcttcaaa
    10621 atatttagta agtgatgatc tttgacttcg ttgccgtcgg gaccaataac aattggtgtg
    10681 gccaatgcca cctgattaga tatgtcttga actaattcat aaacatcaga agattccatg
    10741 atagatgaat ccgtcacata tctttgacca taacgtgtgt agtgtccaaa aatatcctcg
    10801 atatatccac gtttttccat aaaagaatag actgcattcg ataaccgatc acgtaatttc
    10861 aatttttttc accgcctttc tatttatcta tagatggaac ttaggtaatc atctaactca
    10921 tctgaatcaa tatctgtcat ttgattcatc gtttccttat gaccacacaa aaacgccacg
    10981 aacccatcaa tctttttctt tgattgacgt ttacttggcg ctttttgtcc attgatgtta
    11041 gtaattgcta caacgttcaa agtgcaataa aggaataatg gattatcaaa ttgaattcgt
    11101 ttctcataaa acaaccgttc gacatcgtca aaaggagcat tcaatacttt aggatattga
    11161 gcaacttcaa cgcattccaa tcctaagttt tctaatttct caacaagttt gtcactcatc
    11221 gctggatcat aattcacttg ctgaatatca tataaattca tgcagtcctc tatgtactgc
    11281 aaaatctgat cttgatcaat catttttccg tcgcaaaatt caacgaaacc ttgttcagcc
    11341 aaatcactgt aaggaacatt atcttctttt tctcgaaact ctaaatcttc attcggaata
    11401 aaataaagtt gctttacttt aagaaccgct ttaccatctt catcccatgt tgggaagttt
    11461 aaagatacac aggtcaaatc tcgtgtacgt gataaatcta aaccgatata acatggttcg
    11521 ccacttaaat caccaagttc ttgtgtggta actaaacatg gctctacttg atcctgttca
    11581 aagaaattat ccgcgccgtt cacaaataca tccaagtgtt tcgttagaaa ttcagctttg
    11641 gaatgagctg accgttgcgc tgttttaaat gccgattcta aagcagataa atcgactgat
    11701 attccccagt taggattgca catttcccag acttttctat ccgtccaatc ataattttta
    11761 tttggctcat agattagaac aaagtttgaa tcattatcat cacgtttcaa cacttctttt
    11821 gcttcgcgat atacacgcat tccaacagac gacgaacctt tcccagcagt tgagatatta
    11881 aacatcaatg gctgtggtaa agatatctga gcagacttaa aattgtcata ctgttccatt
    11941 ttctcctgtt tatgcaattc atcgttcaat acaaaatatg gattggatcc ctcgatatta
    12001 tcaatattct tggtttgaac aataaattta tttgagtaag ccatatcttc atgaatatag
    12061 tcataagtaa tactagaaac agttcccttt ggccccctaa atattttagt tccttcaagt
    12121 aggattggat tgtttaagat tgtaactgca aaaggtttgg cagcatattg cgcttgagca
    12181 aaatcagatg cgcaagcata gcaatcaact gataacgccc cttctccgta cattgcatat
    12241 cctaatgaac cgacagcaat caatgtttta ccattctttt ttggaatctg gacataagcc
    12301 tcacgagtaa cgcgtacaat ttggcctttt tcattttctt taacccaacc ataaatccaa
    12361 gaataaataa atttttccca gggttctaaa agaaacggtt ttcccaccat ttctcctttt
    12421 gtgtgaacaa taaatgattc tacccaatcc atcatttcat tcgcgcgatc tacatcaaac
    12481 caaatatctt ttcttttttt ccactgatac cagcgatcta ttgctaaacg aacggtctta
    12541 ggatacttct taggtttctt tcttacttct tttgcaaata aatcagcata atttacacca
    12601 ggttcaatca tttttcagca cctgccttct tacgccattt atttctgtgc gctgctagtt
    12661 catctactgg ctttacttct gggcgtgtaa tttcttcatc ttttcttgcg gtcgatcctc
    12721 cagcaattag tctaccagtt tttgctttgt ttgttaatcc taataaatct agagctttca
    12781 tcttcttatc tgcccaagtt tctacttgtt gtgccaatgg atgttttgat tgattagtag
    12841 ctcctgattt attcgtgaat ttttgcgtct ccggaaatcc tttttccttc cacaataaat
    12901 atttgtgttg gtaaatttca aaaatatcca agtatgattc gattaatgga tcaagagtga
    12961 tggtgtacaa atcagacaaa ttcattattt ctaagatacg agctttttca gcacttactt
    13021 tttcatcaat aatcgcttta cgttgcgctt tagtcgtcat ttttgtatac accccccttt
    13081 gttttttgaa aaatttgacc taacgatgcg cgtgactccc cgctacccta tctccccacg
    13141 cgaaaaaatt ttgaaaatga ataggggggc ttcaattgaa atacgaagga aaaacttttt
    13201 tgtcttccgt ttcgttttca acaattggat gacattttga acataatagc atgagattgt
    13261 ttggatcaag cttaagcagt tcgttgtctt tgattggtac aatgtgatgg acgtgtgctc
    13321 tctttccaaa tatgaactga ccacaccgct gacaatgacc accttctctt tcataaataa
    13381 attgacgcat atctttccat gcttgcgttc gatagaatgg tttgttctca tgatgataaa
    13441 cagactttgc ttgctgcttc ttcttgcgtg atctgcttga tctcttgtgt tcagtacagt
    13501 agataccctt tgctattttg ttcgtgcatc cgtcaaactg acagtatttc attctacttc
    13561 acgaataaga tttatgatat cgccttttgc acggatagca ccaggaatat caataccatg
    13621 tttcttagca tatgcacgca attcttttgc agtcatattg tccagttcat ccgtatcttc
    13681 agtggactga tcattagtag cttcgtctcc atcaaaatta gcagcagtgt ttccatcgct
    13741 atcaagaatt tcactgctat gagctatgag ttcgccattg acagcaacaa atgatttgcc
    13801 atcactcaaa attccttttt cttcttttac ttcaaaattt ggttcttgat ctttcggaac
    13861 aactactgtc cttttctttt ctgaatccca atactctgtt ccactaatgg atgttctaat
    13921 tttgatcatt gccattttaa ttctctcctt tgtaatttgt acggattact tttgcgccca
    13981 ttcgctcata ccattcaatc gtttcctgca gattgggtag agtgtgtgac aataaagaaa
    14041 taactaacgt attcattcca gttctgtaat catcattctc cacttgcaca tgtgcatgat
    14101 taccattcca attttctctg tacggactat tctctgcttc aattgctttc ttataaactt
    14161 tagccaaact tgattctact tcaatactaa gtaccgcttc atgaaaatca ttcatttgtt
    14221 tactctccta tcagatattt tgtggtatga tttataaaaa agatgcgagg tgttttaatg
    14281 cttcaagaag ttaatattaa gtatcgtgat cgtcaaaata cagagacaat ttatttgcca
    14341 aatagttgtc cgcattgcgg aaatataatg actccgtatg tgtattttgg ggtttcatca
    14401 tatagtgcgt tcgataatga tagaatcttt ggcgtattag tacagtgtat aaatgatgag
    14461 tgcaacaaat tttatagttt acaatttata aatacgttta atgaagatga aggatattct
    14521 gaaaatattt taattaaaga ttattaccgt ccgccgatta aaatagattt accagaaaat
    14581 attgaaaaag tttctcctac atttgttgat atttatactc aagccacaca agctaaatct
    14641 gagcaattgg aacaaattgc tggagttggt tatagaaaag ctctagaatt tctaattaaa
    14701 gattatgcaa tatctaaaaa ttcagaggat gaagataaaa taaaaaaaat gttattaggt
    14761 aaaacaattg aaaattattt gaatgatttt cctaaactcc aaagattagc caaagctgct
    14821 acttggattg gtaatgatga aacgcactat gttcgtagac atactaataa agatgttcat
    14881 gatatggatg catttattaa atcagcagcg caatttatcg cggctgatta tgatgctgat
    14941 ttagctgatc aatttataaa tggaaataac tttatgccaa ctactaatta aaagaataaa
    15001 agagccacag ataccaatta ataaaacggt tgggatatct tggctctttc atattttttt
    15061 gacactaata gaatatcatg ctgctaacaa tgtttacata gctacaatga ccgaacattt
    15121 agtgaacagt gtaattttag aataaagaga gttgttcttt gtacttctcg ctattgtaaa
    15181 aatcaatttc gttttctttt ttcaatcggt cagcttgtct gtgttcatac tcatctaaaa
    15241 agttcaatgt ctttctaatt tctgcatgtc gctgtctgac atacgatgag ctatacccaa
    15301 cagattcagc aatatcttct aagctcattt gatcaacata tttcatctta acaatttgat
    15361 tatcgatacc agaaaagcta tctatcagca ataacatttc ttctttttgc tcgatcaaca
    15421 attccaattc attttctatc ttttggatat tctcttctaa agaagaagtt ctcgaattct
    15481 tttcgatgcg gacgtttgct aaatcaccac agacccatcg attcaattca agcttacttt
    15541 tattaagatt ccactttaag taaagaatct gctcatctag ttcttggtaa tcttttaacc
    15601 aatgaaatct cacaaacgcc accccttatg gtagaatagt attgcgctgc tatctcgaaa
    15661 ggggtagctt ttttattttt tcttagccgt tccattctgg attgcctttt gttccaaacg
    15721 tcgttttttc ttcttaatct ttgattttgt ttttcccata caattctcct ttccgaaaac
    15781 acattttgtt cgatttttaa tactaaataa aagttggcca gaatttttcg ttcgtcttta
    15841 ctcatctttt tttgaataag attactagca taaaaatagt tagtaccacg aaaatgaaca
    15901 ggtaaatcaa ttacgttcac cttcttcatg ccagttatca gccactagaa tctttgcgtt
    15961 agtgccgcca ccgttttctt ttttgtattt ttcaaatgct ttataagcag ctgtttttga
    16021 tttatagatt tttccggaac cgactgggaa catccatgtg ccctctgtga tagctaagat
    16081 gtatactttc atttaattca cccctttaat ccccaacact acatatccgg acttctgcat
    16141 gtaatcagta atataagtga tctctgcttt gtatgagtca ccagtgtaac taaatgttgg
    16201 tctcgttagt ccaggtaatt catgtttaat gtatttttta tattcacgta gtattaactg
    16261 atcaccaact ttgaagtcac gatcattctt gcggatctcg aattgtttgc gtccgctcgt
    16321 cactgcttca aaatattctg gtagtatttt tagttcgtgg atcattctgt ttcctccttc
    16381 aaaattcgct catcatcaaa aagtgaatct ccacaaaatc cttctcccaa atatttatcg
    16441 ttcatgtata agtcaaccaa atatttgtga tctgcttctc gatattcatt aatattaacg
    16501 atctttattt tgtaaacatt accttcgtcg ctcactatct tgaattgttc tctgactttc
    16561 accaccgttc ctcctctttt tgaaaaaatt ttagtgtttt tgtaagaccc cctaaatcag
//
LOCUS       DACCXW010000057        16615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCXW010000057 DACCXW010000000
VERSION     DACCXW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.057"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="IVY71_001597"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="IVY71_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7985654.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
     gene            complement(680..1666)
                     /locus_tag="IVY71_001598"
     CDS             complement(680..1666)
                     /locus_tag="IVY71_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7985655.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1689..2255)
                     /locus_tag="IVY71_001599"
     CDS             complement(1689..2255)
                     /locus_tag="IVY71_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7985656.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2269..2685)
                     /locus_tag="IVY71_001600"
     CDS             complement(2269..2685)
                     /locus_tag="IVY71_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7985657.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3142..5076)
                     /locus_tag="IVY71_001601"
     CDS             complement(3142..5076)
                     /locus_tag="IVY71_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7985658.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5378..6313
                     /locus_tag="IVY71_001602"
     CDS             5378..6313
                     /locus_tag="IVY71_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7985659.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6403..7506
                     /locus_tag="IVY71_001603"
     CDS             6403..7506
                     /locus_tag="IVY71_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7985660.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7519..8472
                     /gene="menA"
                     /locus_tag="IVY71_001604"
     CDS             7519..8472
                     /gene="menA"
                     /locus_tag="IVY71_001604"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7985661.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IVY71_001605"
     CDS             complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IVY71_001605"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7985662.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10887..12128)
                     /locus_tag="IVY71_001606"
     CDS             complement(10887..12128)
                     /locus_tag="IVY71_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7985663.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(12146..13069)
                     /locus_tag="IVY71_001607"
     CDS             complement(12146..13069)
                     /locus_tag="IVY71_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7985664.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13290..15782)
                     /locus_tag="IVY71_001608"
     CDS             complement(13290..15782)
                     /locus_tag="IVY71_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7985665.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15789..16256)
                     /locus_tag="IVY71_001609"
     CDS             complement(15789..16256)
                     /locus_tag="IVY71_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7985666.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16392..16465)
                     /locus_tag="IVY71_001610"
     tRNA            complement(16392..16465)
                     /locus_tag="IVY71_001610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16429..16431),aa:Arg,seq:acg)
     gene            complement(16523..16596)
                     /locus_tag="IVY71_001611"
     tRNA            complement(16523..16596)
                     /locus_tag="IVY71_001611"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16560..16562),aa:Pro,seq:tgg)
BASE COUNT         4906 a         3477 c         2957 g         5275 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
      601 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      661 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      721 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      781 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      841 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      901 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      961 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
     1021 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
     1081 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
     1141 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
     1201 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
     1261 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
     1321 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
     1381 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
     1441 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
     1501 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg attgcttgtt
     1561 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1621 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1681 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1741 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1801 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1861 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1921 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1981 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     2041 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtctcg ttttttcatg
     2101 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     2161 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     2221 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     2281 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     2341 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     2401 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     2461 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     2521 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2581 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2641 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2701 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2761 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2821 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2881 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2941 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     3001 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     3061 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     3121 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     3181 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     3241 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     3301 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     3361 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     3421 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     3481 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3541 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3601 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3661 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3721 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3781 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3841 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3901 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3961 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     4021 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     4081 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     4141 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     4201 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     4261 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     4321 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     4381 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     4441 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     4501 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4561 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4621 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4681 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4741 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4801 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4861 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4921 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4981 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     5041 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     5101 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     5161 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     5221 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     5281 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     5341 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     5401 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     5461 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     5521 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5581 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5641 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5701 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5761 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5821 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5881 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5941 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     6001 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     6061 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     6121 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     6181 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     6241 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     6301 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     6361 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     6421 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     6481 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6541 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6601 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6661 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6721 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6781 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6841 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6901 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6961 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     7021 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     7081 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     7141 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     7201 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     7261 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     7321 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     7381 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     7441 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     7501 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7561 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7621 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7681 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7741 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7801 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7861 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7921 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7981 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     8041 ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta
     8101 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     8161 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     8221 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     8281 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     8341 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     8401 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     8461 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     8521 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8581 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8641 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8701 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8881 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8941 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     9001 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     9061 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     9121 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     9181 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     9241 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     9301 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     9361 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     9421 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     9481 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9541 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9601 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9661 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9721 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9781 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9841 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9901 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9961 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
    10021 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
    10081 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
    10141 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa
    10201 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
    10261 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
    10321 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
    10381 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
    10441 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
    10501 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10561 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10621 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10681 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10741 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10801 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10861 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10921 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10981 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    11041 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    11101 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    11161 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    11221 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    11281 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    11341 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    11401 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    11461 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    11521 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11581 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11641 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11701 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11761 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11821 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11881 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11941 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    12001 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    12061 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    12121 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    12181 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    12241 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    12301 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    12361 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    12421 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    12481 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12541 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12601 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12661 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12721 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12781 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12841 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12901 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12961 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
    13021 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    13081 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    13141 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    13201 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    13261 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    13321 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    13381 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    13441 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    13501 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13561 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13621 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13681 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13741 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13801 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13861 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13921 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13981 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    14041 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    14101 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    14161 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    14221 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    14281 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    14341 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    14401 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    14461 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    14521 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14581 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14641 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14701 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14761 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14821 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14881 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14941 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    15001 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    15061 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    15121 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    15181 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    15241 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    15301 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    15361 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    15421 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    15481 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15541 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15601 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15661 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15721 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15781 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15841 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15901 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15961 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    16021 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    16081 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    16141 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    16201 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    16261 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    16321 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    16381 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    16441 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    16501 acaaaaagca agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc
    16561 caaaccaagt gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DACCXW010000058        16505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCXW010000058 DACCXW010000000
VERSION     DACCXW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.058"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..546
                     /locus_tag="IVY71_001612"
     CDS             <1..546
                     /locus_tag="IVY71_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP7985667.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="IVY71_001613"
     CDS             735..1403
                     /locus_tag="IVY71_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7985668.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1833)
                     /locus_tag="IVY71_001614"
     CDS             complement(1603..1833)
                     /locus_tag="IVY71_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985669.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1960..3342)
                     /locus_tag="IVY71_001615"
     CDS             complement(1960..3342)
                     /locus_tag="IVY71_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7985670.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="IVY71_001616"
     CDS             complement(3498..4685)
                     /locus_tag="IVY71_001616"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7985671.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="IVY71_001617"
     CDS             complement(4692..5723)
                     /locus_tag="IVY71_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7985672.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5769..7193)
                     /locus_tag="IVY71_001618"
     CDS             complement(5769..7193)
                     /locus_tag="IVY71_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7985673.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            7368..8273
                     /locus_tag="IVY71_001619"
     CDS             7368..8273
                     /locus_tag="IVY71_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7985674.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="IVY71_001620"
     CDS             8364..9041
                     /locus_tag="IVY71_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7985675.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9322..10218
                     /locus_tag="IVY71_001621"
     CDS             9322..10218
                     /locus_tag="IVY71_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7985676.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="IVY71_001622"
     CDS             complement(10273..11643)
                     /locus_tag="IVY71_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7985677.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="IVY71_001623"
     CDS             11881..12627
                     /locus_tag="IVY71_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7985678.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="IVY71_001624"
     CDS             12624..13463
                     /locus_tag="IVY71_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7985679.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14414
                     /locus_tag="IVY71_001625"
     CDS             13464..14414
                     /locus_tag="IVY71_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7985680.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14555..15307
                     /locus_tag="IVY71_001626"
     CDS             14555..15307
                     /locus_tag="IVY71_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985681.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15326..16477
                     /gene="queG"
                     /locus_tag="IVY71_001627"
     CDS             15326..16477
                     /gene="queG"
                     /locus_tag="IVY71_001627"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7985682.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5370 a         3085 c         3318 g         4732 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgcaaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgttactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca
     5761 tcgtgaagtt aatcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctgcattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact tttcttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagcgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa
    14041 gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt
    14101 atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc
    14161 aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg
    14221 ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg
    14281 gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag
    14341 attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa
    14401 caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa
    14461 gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc
    14521 cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata
    14581 tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca
    14641 agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac
    14701 aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt
    14761 agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg
    14821 cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt
    14881 catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat
    14941 tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg
    15001 gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta
    15061 ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag
    15121 tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc
    15181 aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc
    15241 cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag
    15301 ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa
    15361 acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga
    15421 atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga
    15481 tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact
    15541 tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc
    15601 tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat
    15661 cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc
    15721 gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt
    15781 tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga
    15841 gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg
    15901 tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc
    15961 ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa
    16021 aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca
    16081 aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaaa tatatccaaa
    16141 attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc
    16201 tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct
    16261 aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat
    16321 cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct
    16381 tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa acgaattgga
    16441 acatgcccag aaattattgc ggagaaagtt aaaataaaaa aatagttaac attattcaaa
    16501 gtttt
//
LOCUS       DACCXW010000059        16293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCXW010000059 DACCXW010000000
VERSION     DACCXW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.059"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(242..1969)
                     /locus_tag="IVY71_001628"
     CDS             complement(242..1969)
                     /locus_tag="IVY71_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7985683.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1962..3155)
                     /locus_tag="IVY71_001629"
     CDS             complement(1962..3155)
                     /locus_tag="IVY71_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7985684.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3342..3986)
                     /locus_tag="IVY71_001630"
     CDS             complement(3342..3986)
                     /locus_tag="IVY71_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7985685.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4080..4214)
                     /locus_tag="IVY71_001631"
     CDS             complement(4080..4214)
                     /locus_tag="IVY71_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7985686.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4216..6348)
                     /gene="feoB"
                     /locus_tag="IVY71_001632"
     CDS             complement(4216..6348)
                     /gene="feoB"
                     /locus_tag="IVY71_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7985687.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6345..6818)
                     /locus_tag="IVY71_001633"
     CDS             complement(6345..6818)
                     /locus_tag="IVY71_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7985688.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7219..7443
                     /gene="nrdH"
                     /locus_tag="IVY71_001634"
     CDS             7219..7443
                     /gene="nrdH"
                     /locus_tag="IVY71_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7985689.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7602..9761
                     /gene="nrdE"
                     /locus_tag="IVY71_001635"
     CDS             7602..9761
                     /gene="nrdE"
                     /locus_tag="IVY71_001635"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7985690.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9811..10776
                     /gene="nrdF"
                     /locus_tag="IVY71_001636"
     CDS             9811..10776
                     /gene="nrdF"
                     /locus_tag="IVY71_001636"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7985691.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10865..12004)
                     /locus_tag="IVY71_001637"
     CDS             complement(10865..12004)
                     /locus_tag="IVY71_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7985692.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12313..13026)
                     /locus_tag="IVY71_001638"
     CDS             complement(12313..13026)
                     /locus_tag="IVY71_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7985693.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13280..13981)
                     /gene="nagB"
                     /locus_tag="IVY71_001639"
     CDS             complement(13280..13981)
                     /gene="nagB"
                     /locus_tag="IVY71_001639"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7985694.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14215..15747
                     /locus_tag="IVY71_001640"
     CDS             14215..15747
                     /locus_tag="IVY71_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7985695.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15938..16117)
                     /locus_tag="IVY71_001641"
                     /pseudo
     CDS             complement(15938..16117)
                     /locus_tag="IVY71_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16137..>16293)
                     /locus_tag="IVY71_001642"
     CDS             complement(16137..>16293)
                     /locus_tag="IVY71_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7985696.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT         5044 a         3371 c         2854 g         5024 t
ORIGIN      
        1 tttttgagaa tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg
       61 atcgatctct ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt
      121 tcgaagatga gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga
      181 gcttccaaat ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt
      241 tttatttttt ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt
      301 gctgtggatc ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc
      361 aatggaaatt gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa
      421 ccatcgtatg gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca
      481 gatcatattt ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt
      541 ttttgatcgc ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc
      601 agtctttcaa gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc
      661 ctggctcgat tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta
      721 catccatgta ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt
      781 cttcgacttg atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg
      841 aaaccatggc atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg
      901 gcgtcatcgt tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag
      961 caacttctgt atcccattca acataagata agttgatacg ttttgtttct ttggcagatg
     1021 aaaaactccc tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc
     1081 cttgacgttt tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt
     1141 tcaaatgctg tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga
     1201 caaatccatt cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg
     1261 gtgcaccgat catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga
     1321 tccctgccat gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac
     1381 ttgtgctccc gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta
     1441 aattcgtaaa acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa
     1501 ccatcccgat accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct
     1561 gcataaagtc aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga
     1621 tccctaatgg taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag
     1681 tattcatcaa atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga
     1741 acaacgtaag tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga
     1801 taaacaaagg agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc
     1861 caatcgtttg taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat
     1921 tttctaccca gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc
     1981 tcgcttgttt ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt
     2041 ccatcctcta caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt
     2101 accaccatgt ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt
     2161 ttttgcttcc tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt
     2221 ctgtcaacag ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt
     2281 ggtccatcag attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc
     2341 actttggaac gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc
     2401 aatatttctt cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg
     2461 tccccaatcc gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca
     2521 ttggcttgca ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc
     2581 gagaacgctt cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca
     2641 acccgttgtt gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa
     2701 ctagaatttt ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg
     2761 acttctaacc cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt
     2821 tggaaaacca tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc
     2881 gatacatctt ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat
     2941 agacggatca atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct
     3001 tcttttacgt caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc
     3061 tccgttttcg gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat
     3121 atctttgtta aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat
     3181 tcatttttct ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca
     3241 aaaatgaaag aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa
     3301 aaggtttagc caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc
     3361 tccgggtaaa aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg
     3421 atgtcccctg ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga
     3481 gaaattgcga taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg
     3541 tccagatgca ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta
     3601 tgtattcgct tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca
     3661 tcttgttgct tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca
     3721 tcttgatatt gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt
     3781 ccatgccgta cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc
     3841 tcaggagaag cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact
     3901 ttatatcggt tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg
     3961 cggacgattt ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat
     4021 cttactatgc tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat
     4081 catcgcttga tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta
     4141 ccggatttta aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat
     4201 aagaatgtcg ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt
     4261 ggattttggc tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata
     4321 cgtccctaac gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact
     4381 aactaaataa gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt
     4441 catttcccga tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc
     4501 taacaatgaa taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct
     4561 gccattttcg gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc
     4621 tttagcgacc aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg
     4681 ctgaaaaaca actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac
     4741 aggttgagca taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat
     4801 aatcgttcct gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc
     4861 tgaccattga ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc
     4921 aaacaaacgt gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa
     4981 ataggcagaa ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat
     5041 aatcggcaac tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc
     5101 ttgctcgttt tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat
     5161 cagcatagga atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac
     5221 aaaagcgatc ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat
     5281 aatcaattgc ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat
     5341 cagctcctgc atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg
     5401 taaccatgta ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg
     5461 aatagccaga taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg
     5521 attcgttgca atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac
     5581 aacacagagt gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc
     5641 actatcgtcg tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg
     5701 agaaactgct atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc
     5761 ataccagcgt ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc
     5821 ttctaaacgt ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc
     5881 ctgaatcttt tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac
     5941 tggtacacca agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat
     6001 gacatccatc atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt
     6061 taaataaaga tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg
     6121 ttcctgaacc aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata
     6181 gatgccgggt aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt
     6241 ttctactgtt acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa
     6301 caagctagtt ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc
     6361 ctttgtatca agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact
     6421 gtcagttcga ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc
     6481 gtcaatccca tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg
     6541 atcccagtct ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc
     6601 tttttattga caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt
     6661 accagtaaaa tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg
     6721 atcccgatat tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga
     6781 taaacgaaat tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat
     6841 aaatcatgta ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa
     6901 atgagaatca ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga
     6961 tattatgagc gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag
     7021 ttttccacaa ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc
     7081 tcttttttca aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt
     7141 ttgttgtttg acttttttac taagagtttt atactatcta ttatagataa taagaatgtg
     7201 ttagaggagg cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt
     7261 aagatggtca aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat
     7321 gaacagcctg aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt
     7381 acttctgatg cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc
     7441 taacatgact aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca
     7501 gtttttgtcg caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca
     7561 atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac
     7621 aagatgtcag ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc
     7681 ctttgaataa ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata
     7741 cgatgacttt tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag
     7801 aagcagaatt cattcaattg tattcaccat catttattga aaagctatat caatttttgg
     7861 acgagcaaca tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg
     7921 cgttgaaaac caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca
     7981 atgcgcttta ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga
     8041 tccatcaacg atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg
     8101 gcgaattggt gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct
     8161 caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa
     8221 atcttcgtga agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc
     8281 cagtcatgaa attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag
     8341 gagctggcgt ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa
     8401 aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac
     8461 ctgataaatt ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata
     8521 gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc
     8581 atctagttgc aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg
     8641 aaatctcaaa attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga
     8701 accgcgagaa tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac
     8761 aagttcaaac gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata
     8821 tctcatgtaa cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc
     8881 aatctgtgcg tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg
     8941 tcgtaccacc gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg
     9001 gcttacacac ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact
     9061 ttacaaatat ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg
     9121 ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt
     9181 atttcgaaaa atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt
     9241 ttgaaggcat ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga
     9301 aagatggact gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata
     9361 tcaacgatac gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga
     9421 agaaaatcgg taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt
     9481 acacttctgc ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac
     9541 aacacgtcga tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca
     9601 tttatgaatg gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt
     9661 acgctttcca caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag
     9721 aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct
     9781 tattcatatt ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac
     9841 tggaatgaaa ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt
     9901 tggctagata cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa
     9961 ttggaaaaag atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa
    10021 tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt
    10081 ttgaacaata ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt
    10141 acactgaata cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat
    10201 ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag
    10261 aaaattgcca gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt
    10321 tattatttag gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga
    10381 gatgaatctg tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta
    10441 cctgaagcag aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac
    10501 gaaaacgaag aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc
    10561 aagacattcc tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc
    10621 tttccagata ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca
    10681 agcaaccatg acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg
    10741 atgaaagacg acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt
    10801 ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt
    10861 atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt
    10921 ccattcttct caataaatag attttccgga tatttaatat tatcgtctta atcactgcat
    10981 agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata
    11041 ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca
    11101 aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg
    11161 gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg
    11221 ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata
    11281 ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga
    11341 tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg
    11401 ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata
    11461 gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt
    11521 ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat
    11581 ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt
    11641 catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg
    11701 tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa atgattacga
    11761 ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa
    11821 cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg
    11881 aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga
    11941 tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt
    12001 gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg
    12061 cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga
    12121 cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt
    12181 ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa
    12241 tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca
    12301 acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac
    12361 ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat
    12421 atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac
    12481 gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa
    12541 ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc
    12601 aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat
    12661 ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt
    12721 cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag
    12781 ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa
    12841 tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa
    12901 aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc
    12961 gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc
    13021 attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt
    13081 gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta
    13141 tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg
    13201 ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
    13261 tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa
    13321 cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca
    13381 tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt
    13441 tcataattga accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga
    13501 agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt
    13561 caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt
    13621 ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat
    13681 cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga
    13741 catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta
    13801 agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag
    13861 taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt
    13921 tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca
    13981 tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt tttcttttta
    14041 caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc
    14101 gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag
    14161 tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt
    14221 atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc
    14281 gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa
    14341 agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg
    14401 caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca
    14461 atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct
    14521 tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa
    14581 ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg
    14641 agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt
    14701 ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc
    14761 ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt
    14821 caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc
    14881 accacccata tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa
    14941 caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa
    15001 caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca
    15061 acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct
    15121 tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg
    15181 gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc
    15241 atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta
    15301 ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac
    15361 acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga aacaccacta
    15421 caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct
    15481 aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa
    15541 caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat
    15601 gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa
    15661 tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa
    15721 cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg
    15781 tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt
    15841 cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt
    15901 acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc
    15961 tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc
    16021 taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag
    16081 ccgcacctcc aataaagtac ataaacataa atacagaccc gtggtgctag ttaattttaa
    16141 tatcctaaag aatatctcaa agttccttca aaacgtcgtt gtgctcgact taatagaaca
    16201 ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc ctttaacaga acgtgagttg
    16261 aaattttgac aacctaattt ttctaaggaa gaa
//
LOCUS       DACCXW010000060        16291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCXW010000060 DACCXW010000000
VERSION     DACCXW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.060"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(76..732)
                     /locus_tag="IVY71_001643"
     CDS             complement(76..732)
                     /locus_tag="IVY71_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7985697.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            925..1203
                     /locus_tag="IVY71_001644"
     CDS             925..1203
                     /locus_tag="IVY71_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985698.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1222..2430
                     /locus_tag="IVY71_001645"
     CDS             1222..2430
                     /locus_tag="IVY71_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7985699.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2578..2943)
                     /locus_tag="IVY71_001646"
     CDS             complement(2578..2943)
                     /locus_tag="IVY71_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7985700.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3090..3863)
                     /locus_tag="IVY71_001647"
     CDS             complement(3090..3863)
                     /locus_tag="IVY71_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7985701.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4037..4897)
                     /locus_tag="IVY71_001648"
     CDS             complement(4037..4897)
                     /locus_tag="IVY71_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7985702.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4897..6315)
                     /locus_tag="IVY71_001649"
     CDS             complement(4897..6315)
                     /locus_tag="IVY71_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7985703.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6372..6821)
                     /locus_tag="IVY71_001650"
     CDS             complement(6372..6821)
                     /locus_tag="IVY71_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7985704.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7266..7529)
                     /locus_tag="IVY71_001651"
     CDS             complement(7266..7529)
                     /locus_tag="IVY71_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7985705.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7628..8923)
                     /locus_tag="IVY71_001652"
     CDS             complement(7628..8923)
                     /locus_tag="IVY71_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7985706.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8920..10191)
                     /locus_tag="IVY71_001653"
     CDS             complement(8920..10191)
                     /locus_tag="IVY71_001653"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7985707.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10356..11225)
                     /locus_tag="IVY71_001654"
     CDS             complement(10356..11225)
                     /locus_tag="IVY71_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7985708.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11517..13127)
                     /locus_tag="IVY71_001655"
     CDS             complement(11517..13127)
                     /locus_tag="IVY71_001655"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7985709.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14320..15660)
                     /gene="glnA"
                     /locus_tag="IVY71_001656"
     CDS             complement(14320..15660)
                     /gene="glnA"
                     /locus_tag="IVY71_001656"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7985710.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15688..16080)
                     /locus_tag="IVY71_001657"
     CDS             complement(15688..16080)
                     /locus_tag="IVY71_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7985711.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4646 a         3451 c         2769 g         5425 t
ORIGIN      
        1 aatatgagga actatttttc cgtttgtttc tctaatactc aggtcaaaac gctgatttca
       61 caacctcttt gttcatcaaa agataatatt gtcttctaac aagtccatat tcaaaataac
      121 gatatgatgg tctttaacgg tgataatacc atcttttttc agttgtttta aaattctgtt
      181 tacactgcta gcagttgaga tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa
      241 atcgatcagc atcccttctt ctgtccgcac accgaataaa tcatataatt cgtaaagttg
      301 cgtacaaaca gctcccagct ttccgtttaa tagcatttgc tgcatttttt tcatcgaata
      361 aagcagacga tagcggtaat actcttttac atagtgttgt agctttgggt cttggttgat
      421 attttgccaa aagcgcacac gattgattcg ataaaactct gcatattctg attcaactcg
      481 aatattatac ggcgaatcga tgaattgtga atattcgtct tttaaaagag agacgatttc
      541 aagatctttg atatagcgaa ggttaaattc tctcccatca tttgaaataa cacttgcttt
      601 taccgttccc tctttcaaga tatacgtatg ggaatcttcc atcccttcga atgtcaaata
      661 tttatgatat cctttgcgta taatcggaaa atcattttta tccaaatatt cctttaagac
      721 tctactatcc aatgatgctc ccccatccaa actttcatct aaaaagatca tacagtactt
      781 taaaactttt taaagtccaa aggtatttta ctaatgatat taacatttgt taagtacctt
      841 tttccatcac ttatgcgatt tttttccttt tttttattcg ttatactgca gaagtaaaat
      901 tttttaagaa tagagggaaa aaacatgaat tactggatga acacaatcat taatcgatta
      961 gaaacagcat atcaaacaag atttgatatg aaagcttctt tagtcttttt aaatgatgcg
     1021 tatcaaaact cgatcgaatt gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa
     1081 gaatttttaa atctgtttat gtctacaaga gatttattta ttcgccaact tgttgatcgc
     1141 tatccatcca attatcacga tgtagaagtt caaatacaaa aattaaaagc atattcagct
     1201 taaggggaat gtataacata catggaaaag aaattattta ataaatattt tattggaatc
     1261 acattgatca attttattgt ctacctgatt tattatctgt tgatggtcat catggcagtc
     1321 attgcgcaag acaccttaca cgccagcttg agccaagcag gttttgcttc tgggatctat
     1381 atcatcggaa cattgctggc acgcttattc atggggaaac agttagaact atttggtcgg
     1441 aagtttacct taagaggcgg cgcgatcttc tatctccttt caacattagc atatctttat
     1501 acgccaacta tcggggcatt gtatttagtt cgattcttga atggattcgg ctacggtaca
     1561 gtttctacag ctacaaatgc tatcgttact gcatatatcc ctgcatctaa aaaaggggaa
     1621 ggcatcaatt attatggatt aagtactagt ttagctgccg ctgttgggcc atttttagga
     1681 atgattttgc tgaatatgac tgattttcgt ttcattatct ggttttcaat tgtactggtc
     1741 ttctttgtta cgattgcctg ccttgtcttt cctgtaaaaa atattccatt atctgaagaa
     1801 caaaaaatat ctcttcgctc atggaatatc aatagtttta ttgaaaagaa agcacttttc
     1861 attacaggaa ttgccttttt gatgggtctt tcttattcaa gtgttttatc atttttatct
     1921 tcttatacaa aagtaatcca cttagttgct gctgggtcat ttttcttcgt cgtatatgct
     1981 ttggtcatca catttacacg tccattgact ggaaggattt tcgatgtcaa aggagaacaa
     2041 tacgtgatgt atcctagcta tttattctta acagcaggat tatttttact gagtgtaacg
     2101 acgaatagtg tgacattgct tatctctgga gcattagtcg gcctaggtta tggtacgttt
     2161 atgtcaaatg gacaggctgt ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct
     2221 ttatctacct actttattgg tttagaccta ggcttaggcg ttggtcctta tttgatgggg
     2281 atgctaaaat ctcttacttc atttagagga ttgtatgtga ttgccggagt cattccattg
     2341 atatgtacag cactctatct tgttaatggc cgaaaacata gtcatgaagc agagggcgaa
     2401 ttgattgaat cattgaaaaa tgaaatttga tcttgatgaa tagataaaaa aaaccgttca
     2461 gatctgaaaa taatctgagc ggttttttga gtaaaaatag tttatgatta ttcttaatta
     2521 aacatctatt tatttttaaa gctttcattt atcgataaga atagtttaac tgttgattta
     2581 ttccattttc cgttcatctg gtacgatgcc ttgacttagc aaccacttgt ggaagaacca
     2641 ttctgccaca ccgatcaata gagcaggaat caatactttc atccaaatat cgactgtatc
     2701 attcataaaa tatagataga gccacaagat aactgcagct gaaccagcat ctgcgatcgt
     2761 cgctgcgatg ttccctactt tacgtaagat caacagatcc cctagcacat agatcatgac
     2821 tacaataatt accccaacaa tcgttgaatc cataaagcta acaccgtata aacctgtcag
     2881 cacgacccac agaatcgccc agatcatgat tgccttcact accaacgcct tcacatgttt
     2941 cattttctgc actcctttct cctggataaa aaagtaagat tatctaacaa gataaccttt
     3001 tcattattat accatcggct gagagaagcg agcaaagata aggtcctatc taaaaatgag
     3061 gaagccactt agaacattca cgtaataatt tatccgtaca gacattcgat atggtgttcc
     3121 tttactttcg catactgttc ttttgtcagt tctttacttg tcaccagata atcgatttca
     3181 tgaaaagcaa tggcttgatg catgaattta tgtgcaattt tttgctgtgt aactaaaacg
     3241 gcaagccggt ctgattgttt tctaaaagct tttttgacca ttgcctctcc ttcatgagtt
     3301 tctgttactc cttggctgaa atctagcccg cgacaactca ttatcgctaa atccgcataa
     3361 tatgacaatg catcgttata ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt
     3421 gcccctccca atacagatac ttttgccgtt gtatattcac tcaacatagc agctgtgacg
     3481 atgccattcg taatgatctg caaaccatca aatcttgcta ggtttctgac taagtgtaaa
     3541 gaggttgtac tggaatccaa gaataagacc atatcatctc gaatcaaaga agaagctgta
     3601 tctgcaatct tacgtttttc tgtttcttct tctaatttgg tgatcttcat caggtaaggc
     3661 acgtcaattt ttcgattgtc aataagcatg gcgccaccgc gaactctacg gatcaatcca
     3721 cttttttcca gaaaaacaag gtcacgttta atcgtggctt cgctataatt tactaactcg
     3781 atcaaatcgg tgactcttgc aaattggttg ttttttagat aattcacaat ctcctgctgt
     3841 ctattctcca cttcattcaa cataaaatca ccccatttac tttatatagt aacaggaaaa
     3901 aagaggttgt gacagaagtg attggcttcg agaaataaga agaaattttt cgaaaaaact
     3961 acttctgaac atcgtgtgtg gttaagaggg tatgacaaga ctattgtcat accctcttga
     4021 ccacacattt ttatttttag tataaactag ccttgcctac tgtgttcaaa atttccattt
     4081 tatgtttgac cacttcttgt acctttttat tagctgctgg ataaattgca ttcggctcgt
     4141 acatcgatgg attttcactc aatatttctc tcaaagcatt gaaaaagacg cttttaagat
     4201 ctgagctcaa attcactttg gcaactccgt attttactgc ttcgctgact tctgcgtccg
     4261 gattacctga accgccatgt aaaacaaatg gaagatcgat ttgttggttc aattctttca
     4321 gtaagtcgat atttaatttt ggagttctgt ttttcggata caagccatgc gctgttccaa
     4381 ttgctacagc aagtgtatcg attcctgttt ctttcacaaa aatcttcgct tgatcaggat
     4441 ctgtataaat gattgtttca gcacctcctt cagcagaacc attattccca atcgttccaa
     4501 gctcagcctc cactgagaca tttaccgcat gagctaacgc aaccactttt ttcgtaatct
     4561 gtacattgtc gtcgaatgat ttcaaagacg catcgatcat cacggaagaa tagccgtttc
     4621 gaatcgcccg taagacatct tctatcgtac cgccatgatc caagtggata acgatcggca
     4681 gtttacttgt ataagcatag gctcttactg ccgcaacaaa ctcatcactt aaataggcga
     4741 tttcatcagg atgaatttct aagattactg gtgcttctaa tctctctgct tcttcgatga
     4801 ttgctttcat catatcatag ctgcaaatat tgaaggctgg tactgcaaat tgattctctt
     4861 tagccacttt caataactct ttcattgtat ataacattac gcttccacct catttgttag
     4921 attttcattt ttcgtccact tgtcttgttt tttctctttc actgtttttt tcaatagtcc
     4981 aagcataaag gctgtagtga ctgtacctga tgcaattgcc aagacataca taggtaaatt
     5041 gttcataaca tttggaatta gcatcaccca aatccctcca tgaggtgcca ttgaagtaac
     5101 tcctactgcc atggaaatcc ctgcagcgac tgctgaacct gtcatcaaac taggtatcac
     5161 gcggatcggg tcagataccg caaacggtat agcaccttct gtaatatacg aagcgcctaa
     5221 aatccagcat gattgacctg aagtccgttc ttctttggta aatttatgtt tgaataataa
     5281 agtggctaaa gcaataccta ggggaggtgt cattcctgca gccatacaag cagcaatcgg
     5341 tgcatttact ccagctgaca tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg
     5401 cccacccata tcagctgcca tcatgatccc gataagtaat ccgaacaata gcccggatgt
     5461 atttcccaag ctattcagcc aatttgtcaa agcatccaac acccatgtga tcggttgttc
     5521 aatcacaaat accatcaaaa gcccaataat taagatcgat aaaaagggga cgacaatcag
     5581 ctgataaata gcagaaaatt ctttttttac gctaatttta tttgccagaa aatcacagat
     5641 ataccctgct agcacgccac caatcattcc gccaagaaaa cctgaaccac ctgtagaggc
     5701 cagtaatccg gcaacaattc ctggggcaaa acctgctttt ccagcaatcg atgtagcaat
     5761 ccccgcgcct aacgcaggaa ccatcatggc aaacgctgta tctcctccga tccttgaaaa
     5821 tgcagctgct aacacattat agtctgctga gttgggatca gccgctgtaa tcccaaaggc
     5881 aaaactcagt gcgatgatga ttccgcctgc gatcacaaat ggaagcatgt aattcacacc
     5941 attcatgaaa tgattataga ttgtcggctt tttctcattt gcagctggtt ctttttcatt
     6001 agacacaacg aatacgccaa tatctctcaa tgcttcgtct attactactt gcggttcttt
     6061 gattgctcgg cttgtagaga cacgctttac ttttttcccg gcaaatcggt ccaattcgat
     6121 ttctttatct actgcaagaa tgattgcatc tgcctctttg atttcttgtt cagataactg
     6181 attttctgtt tttacacctt gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt
     6241 tacttctaat ttttctgccg acatataagt atgagcaatt ccagtcgggc aagcagttac
     6301 accaacgatt ttcatgatta ttttcctcct ttttcttcac tatcgatact cggacaaaga
     6361 ctctactatt ttcattcttg actaatctgt ctgatgatat ctgctggcgt agtggcttct
     6421 tttattccct ttctaaaatc tgcattcatc aatcgtctgg agagtgtgct gagaatctct
     6481 agatgcatat ctgctgattt ttcaggaact acgatggcaa aaatatattc aattggcatt
     6541 tcatctatcc cgctatagtc cgttaatttg gctgtatgaa aaaaacacag tgctggtttt
     6601 gtcacatatt tgctttttgc atggggaatc ccaaatccat cttctattcc tgtggagatc
     6661 tcctcctctc gtttgaatag tgccttaacg actttttgaa catttcttac cttatttgtt
     6721 tttttcaaca catcccctat tcgctcaaat aatgatcctt ttgaatctat cgatggatcg
     6781 aaaacaatca gttcttctgc gatcaattga tcaaacctca taaaaaacct ctcccccaca
     6841 aaaaatgtta acgttttctt ggaactgagg tcagtatagc atcgctagct gtaccgttca
     6901 atattatgaa tgaacagagg aaaaagaccc atcatccgcg tgtataagtc attaaaatga
     6961 acgatctagc tcacaaacaa tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg
     7021 aaagcttagc ttctaatttt aggtaaatgg ttttttactt cgaaataacc cctcttcatt
     7081 tcgttaatat aaggtgtttt tatcaagtgg ataattaaaa ctatatacta gtttttcatt
     7141 gttttgtgca ttatgatcct acgacttttt cagcatcaaa cgaagtttta aattggcaaa
     7201 aaacataaaa aaacagacta tccctatcag atagtctgct aaacgttgag ttatcagcgt
     7261 ttttattatt ccgcagcgtt tgcgtctttg agaccgtatt ttttgttgaa acggtccaca
     7321 cgtccgtctg cttgtgtgaa tttttgacgt ccagtgtaga atggatgtga gtcagaagtt
     7381 acttcgacac ggatgactgg gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa
     7441 gtttttgttg aacctgacaa gaatttgaaa ccagttgttg aatccatgaa tactactggg
     7501 tggtaatctg gatggatgtt ttctttcatg attttttcgc tcctttgccc tgctccattt
     7561 gctgaaccag agttgatttg tcgatttaca acattaacat cttaacatgt ttcagtcaag
     7621 gttgcaatta tcttttcaga tttctggtcg gatttttttt accaaaggag acatcttgaa
     7681 attctttgaa aaattgctga ttgttttttg ttttacgcaa aaagcggatc agttgttccg
     7741 tatattctaa tgaatctcca gtcatatttt ttcgaagttt ccaaatttct tctaattctt
     7801 cacttggcat caataattct tcttttcggg tacctgattt tttgatatcg atggctggga
     7861 agatccgacg ttcagataat tcacgtgaaa gttgtaattc tagatttcca gttcctttga
     7921 attcttcata gatcacgtca tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa
     7981 tcgtcaaact gccaccttct tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat
     8041 acaatgcggc tgggtcaatc ccgccactca atgtacgtcc gcttggtgga acgactaagt
     8101 tataggcccg agcaagtcta gtaatactat ccatcaagat aacgacatct cgtttatctt
     8161 cgactaaacg cattgcgcgt tccaatacta attcagaaac tcgagtatgg ttttgcggct
     8221 gcagatcaaa tgtagaagat accacgtcgc ctttcacgct tcgttctaga tccgttactt
     8281 cttccggtcg ttcatcaatc agtagaacga ttaattctac ttccggatag ttttcggtga
     8341 taccatttgc gatttctttt aatacagaag ttttccctgc ttttggcggt gcgacgatca
     8401 atccacgctg accaaaaccg attggagaaa agatatcgat cattcgggta gatagtcttt
     8461 gcggtgttgt ttccagttga agctgtcttt gcggataaag aggtgttaag gctggaaaat
     8521 gcggacgttc ttttgcttct tccggatctt ttccattaac gctttccacg tgcatcaaac
     8581 cataataacg ttccgattct ttaggtggac gggcttttcc tgccactttg tccccatttc
     8641 tcaaactgaa tcgtctgatt tgtgaggcag aaatatagat atcttctgca cttggtccat
     8701 aattgattgg ccgtaaaaaa ccgtaaccat cttgaccaac gatatccaat attccttcca
     8761 tataataaaa tccttgtttc tctgcttgtg cacgaatcac agctagagat aattcctttt
     8821 tattcatctt actgtaataa ggaattttaa attctttggc ataactataa acttctttta
     8881 acgttttgtt ctctagttct gccattgtca aataatcgct cattcagctc ccacttcctc
     8941 tatcatttca attgatgccc ctaaggctgt tagtttctcg ataatatgat cataaccgcg
     9001 taagatataa tctacattgt aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac
     9061 taaacaagca cctgcccgaa gatcagaagc cacgacttct gtgccgtgaa gtttatttgg
     9121 accattcacg atgatcatat ttccttccac ggtaatatca gcgcccattc gtgctagttc
     9181 cggcacatgg ttcacacgtt tcgcatagat cgtatcgatg atttctgaag tgcctgtagt
     9241 catcaatagt aacgcagtaa gtggctgctg cagatctgtg gcaaatcctg gataaggata
     9301 ggtcatgatg tttgcgggtt ttaattcttt cgaggggtaa acttcgattt catcttcgct
     9361 gattttcata tggacaccaa tttcctgcaa ttttgctata aagctttcca aatgttcaaa
     9421 gatcacattt ttcactttga taccatttcc tactgcagct gctaatgcta gataggttcc
     9481 tgcttcgatg cggtcaggaa tcatgaaatg ccgacagcca tgtaatgttt cgactccttc
     9541 gatacggatc acatctgtac ccgctcctcg aacttttgcc cccatattat tcagtaaagt
     9601 tgccacatct atgatttccg gttctcgtgc agcgttttca attactgtct gtccttttgc
     9661 ttttacagca gctaacatca cattgatcgt tgctccgact gaaaccacgt ccatgaaaat
     9721 ccgattgcca cgcaatcctt tattttctgt ccgtaaatac atggcaccat gttcatttgt
     9781 cacttcagca cctagagctt caaatccttt gacgtgtagg tcaatcggtc tagggcctaa
     9841 ataacagcca cctggcaggc ctactaccgc ttcgccaaat ttaccaagca gtgttcccat
     9901 aaaataataa gaagcacgca aactattgat ttttccgtgc ggcatcggaa tcgatacgac
     9961 ttctcgagga tcgatttcta gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc
    10021 taaaatttca attaatgaat gaacatcttg tatgtccggc acaccttcca agatcactgg
    10081 ggaatcagct aagattgcag ctggaatcaa tgcgactgca ctattttttg caccgctgat
    10141 cgttacttca ccagctaaag gtcggtttcc tttgattacg atttttttca tctgtttcca
    10201 ccttgtttat taatcttttt tcatggatat gcttgtattt tatcatagat aaaaaaaaga
    10261 aacgagcatg gtctgccgtt ttaagtaaaa aagaatggag agcgacttca aaagtcgttc
    10321 tccattccta tatatcgtat ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac
    10381 cagtcgatat gagatttagc atcttcaacg attgctgctt tacctggagc taataattta
    10441 cgagggtcga atcctttacc ttctttatct ttaccagctt cgatatattt acgagtagct
    10501 gcagcaaatg ataattggaa ttcagtgtta acatttactt ttgagatacc cattgagata
    10561 gctttttcga tttgatcttg aggaatacct gatccgccat gtaatactaa aggcatgtct
    10621 ggacctacag catcagcaat tgcttgtaag tggtcaaaag ctaatccttt ccagttttct
    10681 ggatattgac catggatgtt accgataccg caagctaagt agtcgatacc tgtagcaacc
    10741 atttgtttgc attcttcaat atcagctaat tcgccgttac cgatgattcc gtcttcttct
    10801 ccgccgattg aaccaacttc acattcaact gagatacctt ttgcatgtgc tttttcaaca
    10861 acgtctttag ctaatttcaa gttttcttca aatggtagat gtgaaccatc gaacattact
    10921 gaagtatagc ctaattcgat acattccaaa gcagcttcgt aatcaccatg gtctaagtgg
    10981 atagcaactg gaacagtgat gttcattgct tcaactaaat cagtgattaa atctttacag
    11041 attttgtaac cacccatgta tttagctgca cccattgatg tttggataag tactggtgct
    11101 tgttttgctt cagccgcttc aaggattgct tgagtccatt ccaagttgtt tgtgttgaat
    11161 ccgccgacag cgtaaccgcc tttacgagca gcttttaaaa attcagctcc tgatactact
    11221 ggcataataa aattcctcct aagttttgta aaactaaatt tgttaagaca attcttaacc
    11281 aacgacccta ttttaccaaa catttcggta gttttctact aaaatcaatg aaaatgaaaa
    11341 ttttcaagaa gttttttgca aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc
    11401 ttttgctttc tatctttttt cctacaaata ataacaaatc agaaaaagag aaaagtgaca
    11461 agaaaattgc ttatcccctt ctcttgctac ttttctcttt ctatcaagtt caaaaattat
    11521 tttgtttcgt gatctgctaa tgatgctccg acaaatgctt tgattaattt ttgcgggcga
    11581 tttggacgag aaatcaattc tgggtggaat tggcatccca caaagaattt tttctctggc
    11641 agctctacga tttctactaa acggttatct ggtgagacac ctgaaaagac tagcccttgt
    11701 tcttcaaaca attgacgata tttgttattg aactcatagc ggtgacggtg acgttcttgc
    11761 acaacatcgg ctccatcata tgcttcagcc gtcttcgttc cttttttgat tttacatggg
    11821 tataatccaa ggcgtaaagt tccacccaaa ttctcgatgt tttcctgatc agccattaaa
    11881 tcaataatat tgtttgctgt atctggattc gtttcagctg atgacgcatc ttctaatcca
    11941 acgacgttac gtgcaaattc gacacaagcc atctgcatac caagacaaat acctaaaaac
    12001 ggaacgtcat tttcgcgagc ataacgaatc gcttcgattt tcccttctac tccgcgatca
    12061 ccaaatccac caggaaccaa gattccatct gcgtctttca aacgttcttc tacattttct
    12121 ctcgttaatt cttgtgcttt gatccaatcg atctcgatgt ctgaattata ggcgaaacca
    12181 gaatgtttca atgcttcaac tactgagata tatgcatctg gtaactcgac atatttaccg
    12241 acaagcgcaa tccgtacttt tttcttcaag ttcaagacac gttcttctaa ttctttccat
    12301 tctgtcatgt ctgcttctgg tgtatctagt tttaaatggt cgcaaacaat ttgatccatt
    12361 ccttgtgctt gtaaaactaa tgggatggag taaagtgtat ctacatctgg cgactcgata
    12421 actgcttctg gttcgacatc acaaaattgt gccaatttgt ttttcacgct ttgagaaaca
    12481 ggttgctctg tacgtaccac caaaatattc ggttggatac ctaaaccacg tagctctttt
    12541 acactgtgct gcgttggttt agttttcatt tctccagcag ctttcaagta aggaatcaat
    12601 gttgtatgga tatacataac attatctgta ccgacatctg ctttcatttg acgaagtgct
    12661 tcaaggaatg gcaaagattc tatatcccct actgttcccc ctacttctgt aatgatcaca
    12721 tctgaatcag tcattttagc tgctcgcatg attttttctt tgatctcatt tgtaatatga
    12781 ggaataactt gaacagttgc tcccaagtat tcgcccttac gttctttacg caaaacttca
    12841 gaatagattt ttcctgtcgt cacgttggag tatttgttca aattgatgtc aataaaacgt
    12901 tcgtagtggc ccagatccaa atctgtttca gcaccatcat ctgttacgaa tacttctccg
    12961 tgctgatatg ggctcatcgt ccccggatcc acgttgatgt atggatcaaa tttttggata
    13021 gttactttta agccgcggtt tttcaataag cgtcctaaag aagctgcaac aatccctttt
    13081 ccgattgatg aaacgacacc gccggtcaca aaaatatatt ttgtcataag ttcaaaaact
    13141 cctttttaat ttatgtgcag gaaaagtctc agacaataaa caaagctccc tattcttcaa
    13201 taagaatagg gagccgaaat ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg
    13261 tataatacaa gctgactaag ggaaagtcaa gtactcaaat gaatactatc cgcttacaat
    13321 tttttctttt tcgatatcct ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa
    13381 aatttaaccc acataataag cttgatataa acatagctaa cgttaaacct aagcaaatca
    13441 gaaaatcgtt actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta
    13501 tgatagataa tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat
    13561 atagcttatc gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa
    13621 acgtagtcca cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca
    13681 aaacaattcc actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg
    13741 attcattgaa ggtaacgaat cacataagta aaaaataagg agatgcgaca aaagtcgtgc
    13801 ctccttattt ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    13861 ccatcaaaca tgagaagctg tttttgaaat tatttcttgt gattcggagt taaatggtag
    13921 gctcacaacc tctggataaa cggtattcaa aagccagttt ctcggtatta agccaaattt
    13981 atgcaaaaat gtagaaagcc attttcacaa aatttttctt aatactcgaa actaaacgtc
    14041 tttttcaaaa cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagttcag
    14101 ccaattcaaa aacaagaaaa gctgtttttg cttggctatt cttattactt gttgcagggc
    14161 agctttttca caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc
    14221 aaaaaacggc aaaatatgag gaactatttt tccgttagtt tctctaatac tcaggtcaaa
    14281 acgctgattt cacagcctct aattcacggt attcctgttt taataaagtt ctagatattg
    14341 ttctctttcc cactcggaaa cagtttgtct gaatgccgcc cattcgagac gttttgcttc
    14401 aacaaaattt gagaagatat gttgtcctaa tgcgtcaatc attacattat ccttacgcaa
    14461 ttctttgata gcattatgga tagttgaagg taagtcctgg atatgtgcag cttctctttc
    14521 ttcctcatcc attacataaa tgttacggtc aacagctgct ggcggtgtga tctgattacg
    14581 gataccgtct agtcctgctt gcaacaatac tgccattgcc aagtatgggt ttgcagatgg
    14641 atcaactgac cgcaattcca aacgtgtgga taaaccgcga gattctggta cacgtactaa
    14701 tggcgaacga ttacggccac tccatgcgac ataaacaggc gcttcataac ctggaacgag
    14761 acgtttataa gaattgactg ttggattgca aacggctgtg taggcacgag catgtttcag
    14821 caaaccacct aagaaatgat aagctgtttc acttagttcc aattcaccgt ctttatcata
    14881 gaaaacattt ccttcatcat tgaacaaaga catattgcaa tgcataccag aaccattgat
    14941 cccaaataac ggtttcggca tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt
    15001 gacaacgagc ttgaaggttt ggatattatc acaagcatct accacatctg catatttaaa
    15061 gtcaatttca tgctgtcctg gcgctacttc atgatgtgaa gcttccactt caaagcctag
    15121 actttctaat tccaacacga tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa
    15181 atcaaaataa ccacctttat cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt
    15241 aaataagaag aattctggtt caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat
    15301 atctttcaat gctcgtttca aattcccgcg aggatcgccg gcaaatggtg tcccatccgg
    15361 attatagata tcacaaatta aacgagcaac ttttccatga gcactttccc acgggaagat
    15421 catccaagtg gagagatctg gatacaaata catatcgctt tcttcaattc gaacaaatcc
    15481 ttcaatcgaa gaaccgtcaa acatcatttt attttctaat actttgtcga tctggctcac
    15541 tggaacttct acatttttga tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg
    15601 tacgttttct tcctcaatga ttcgtttgat ttcttcacct gttatctgtt tctttaccat
    15661 ggtatccctc ttcctttttt aatttattca gtcaaagaat tgtctttgca atccttgcga
    15721 ctgataatga cctgttttgc ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg
    15781 tcgaacgtca tgatctgtca acggcttctt gttttttgct tcttgttttt tcgcctgtgc
    15841 catttcgtat acacgtttga tcccagccat attcaatccc tctgaaagat agtctttaat
    15901 ctcaagcaac atatcaatat cattcagcga atacatacgt cggtttcctt cgcttcgttc
    15961 cggatgaact aaatcttgtt cttcataata gcgaatttgc cgagcagaca aatcagtcag
    16021 cttcataact gtaccaattg gaaatacgga catagatcgg cgcaattcct tttctcccat
    16081 cagacgtccc ctttcgtttt ataagttggc taaagtgtac caattaaaaa aaatattgtc
    16141 aactgcatat gttaggtttt ctaacatgag atctctattt tttataaaat aacaaaaaaa
    16201 taagaacaca aaaaaggtat ctagttgatt ttcgtaccag atacctttta ttttttatta
    16261 tactgtacta tcctttatta gtgtttttat t
//
LOCUS       DACCXW010000061        16179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCXW010000061 DACCXW010000000
VERSION     DACCXW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.061"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..195)
                     /locus_tag="IVY71_001658"
     CDS             complement(<1..195)
                     /locus_tag="IVY71_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985712.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKK"
     gene            complement(183..812)
                     /locus_tag="IVY71_001659"
     CDS             complement(183..812)
                     /locus_tag="IVY71_001659"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7985713.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(819..1535)
                     /gene="pyrF"
                     /locus_tag="IVY71_001660"
     CDS             complement(819..1535)
                     /gene="pyrF"
                     /locus_tag="IVY71_001660"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7985714.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1532..2464)
                     /locus_tag="IVY71_001661"
     CDS             complement(1532..2464)
                     /locus_tag="IVY71_001661"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7985715.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2461..3237)
                     /locus_tag="IVY71_001662"
     CDS             complement(2461..3237)
                     /locus_tag="IVY71_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7985716.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3291..6476)
                     /gene="carB"
                     /locus_tag="IVY71_001663"
     CDS             complement(3291..6476)
                     /gene="carB"
                     /locus_tag="IVY71_001663"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7985717.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6481..7557)
                     /gene="carA"
                     /locus_tag="IVY71_001664"
     CDS             complement(6481..7557)
                     /gene="carA"
                     /locus_tag="IVY71_001664"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7985718.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7562..8842)
                     /locus_tag="IVY71_001665"
     CDS             complement(7562..8842)
                     /locus_tag="IVY71_001665"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7985719.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8861..9787)
                     /locus_tag="IVY71_001666"
     CDS             complement(8861..9787)
                     /locus_tag="IVY71_001666"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7985720.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9800..11083)
                     /locus_tag="IVY71_001667"
     CDS             complement(9800..11083)
                     /locus_tag="IVY71_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7985721.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11097..11636)
                     /gene="pyrR"
                     /locus_tag="IVY71_001668"
     CDS             complement(11097..11636)
                     /gene="pyrR"
                     /locus_tag="IVY71_001668"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7985722.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11871..12782)
                     /locus_tag="IVY71_001669"
     CDS             complement(11871..12782)
                     /locus_tag="IVY71_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7985723.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12779..13243)
                     /locus_tag="IVY71_001670"
     CDS             complement(12779..13243)
                     /locus_tag="IVY71_001670"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7985724.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13248..13946)
                     /locus_tag="IVY71_001671"
     CDS             complement(13248..13946)
                     /locus_tag="IVY71_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7985725.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13933..14319)
                     /locus_tag="IVY71_001672"
     CDS             complement(13933..14319)
                     /locus_tag="IVY71_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985726.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14466..16136)
                     /locus_tag="IVY71_001673"
     CDS             complement(14466..16136)
                     /locus_tag="IVY71_001673"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7985727.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT         4358 a         3730 c         2884 g         5207 t
ORIGIN      
        1 cttctttctg ctaaccagtc ttagaaaaat gaattttaaa aaaatcaaaa actgatcacg
       61 atagaagttt cttttcatac ctgatgaaaa gatgcttctc gcaatcagtt tttggatttt
      121 tgctattcca attgatgatt ctggagacac aaatcggatt tctctatctt catcaagcgc
      181 gcttaaatct gccaattttc aggatctttt ttccattctt ttagcagagt taattcttgc
      241 tcatcgatat aacgatcttc taaagctgct tcaatcaata cagaataatt ggtcaacgtc
      301 ataagaggga tttcggcttt ttcgaaattt gcttttcctt ttggcaactc atacgtaaag
      361 atagccgcaa caccaaggat atccgcacct tctctttttg ctgcttcagc tgcttccaag
      421 acacttccgc ctgtagaaat caaatcctcg atcacaacca tcttctgacc ttcaaatata
      481 cgtccttcga tttggttacc tttcccatga tctttcgcct tacttcgaat atagaccatt
      541 ggcaaatcca gtatttccgc tacccatgcg gcatgaggga ttcctgctgt tgcagttcca
      601 gcaatcactt gtacttcagg gtaagcttct ttgattttgc ttgctaatcc ttttgcgatc
      661 tcttttcgga cttttggata gctcatcgtg attcggttat cacagtaaat tgggcttttg
      721 attccacttg cccatgtaaa aggattactt ggatttaaaa atactgcttc aatttctaat
      781 aagtcttttg cgatactttg ttcaacattc attttaggtt agctccattc tgtttgaatc
      841 gtttgatatg ctttataagg attagttgac tgagtgattg gtcgacctat aacgataaaa
      901 gttgagccga tcttttttgc ctgagaaggt gtgactactc ttgtctgatc tcctgcgact
      961 gttccttctg gtcttatacc cggagttaag caaacaaatt cgtctcctgc catttctttg
     1021 atcgcttcta cttcccaagc agaagaaacc acaccatcta atcctgcttt tttggcacag
     1081 ctagcataat ggatcacact tttttctaaa gaaacttcga ttagttgatc ggcatgcatt
     1141 tcttcttcac ttgtagaagt aagctgagtg atagctagaa gtagcggacg ttttccacct
     1201 tctggagtcc cctcttctaa tcctcgcatt gccgcctcca tcatacgaat accgccagcg
     1261 gcatgaacac aggtgagatc aacaccaagc tttgccaatc cccgcattgc tttttcgacc
     1321 gtattcggaa tatcgtgaag tttcagatct aaaaatacat tatgaccagc ctcttttaga
     1381 taacgtacga tttcaggtcc ttcttggtag aacaattcca ttccgatttt cacaaacaat
     1441 ttttcttctt tcgggaaaaa tcgaagaaag tcttctacct cttgcctagt tgaaaaatct
     1501 aatgcaataa ttggtcgttg gtctagttga ttcatcgttc acgttcctct ctcacttctt
     1561 tgattaatct ttccagcgat tcgatgccta gttcgctcat tcgcacaggg agctctttaa
     1621 ttagtttagg acagatatac ggatcggtga aattcgcggt ccctatagct acagcacttg
     1681 ctccagccat aaacatctca agaacatcat ccactgtctg cacgccgccc atcccaatga
     1741 ttggaaggct ggatatactt gctacttggt gaatcaaacg aatagcgaca ggcttgattg
     1801 ctggtccgga gagtccacct gtttgattgg ctaaaatcgg ttttcttgtc ttaaggtcaa
     1861 tccgcatacc aagaagagta ttgatcatcg taaacccatc tgcccctcca gcttcgattg
     1921 cctgagcgat tggaacgatg tctgttacat ttggggacaa tttgacatag acaggaacag
     1981 aagaaacttt ctttactgct tgtgttaatt gaaaagcaac atcagaatct gttccaaaag
     2041 caattccgcc atgtttgaca tttgggcaag aaatgttcag ctcgattgct ttcacatttg
     2101 gtgcttcgcc aattttgcta caaacttcta catagtcttc ttcacaagca ccagcaacat
     2161 ttgcgatgat gggtaactca ttgtactcct ctaattctgg aagaagttta tgcataacaa
     2221 cgtctagacc aggattttgc aaaccaattg cattcaacat gccagatggt gtttccgcca
     2281 ctcttggcgt gggattgcca aagcgggcat ttggagtcgt tgccttgacc atgatcgatc
     2341 caagctgatt caaatcatag tattttccgt attctttgcc aaaaccgaaa cagccgcttg
     2401 ccggcatgat aggatttttt aattctaggc caggaagttt gaccgctaaa ggattctcat
     2461 tcatataatt acctctccta ttttaaaaat cggtccttcg tcacaaactt tcacactttt
     2521 tgttcctgtt tcatcttccg gcgtatgaca aacgcaagca tagcaagccc ctatcccaca
     2581 tgccatgcgg gattctaatg ataactggac attttgatgc gaagaaaaca gctgttccac
     2641 tgtcttcaat aacccgtttg aaccacatgc aaaaacagct gtaggttccg tcttcgttcc
     2701 taacaataaa ttaccgacat ttccgtgcat accgtatgtt ccatcgtcag tagcgattct
     2761 tgtttctcct agacgattaa attctttttc ataataaacg acttcatgtg tggcaaaacc
     2821 taaaaaatgc gttggcttaa ttcctttttc gactaactgt tttgataact catatagagg
     2881 agggacaccg attcctccac cgattataaa tacttcgtct cctgtatgaa gattggacaa
     2941 atcaaaacca ttgcctaaag gacccatgac atctaaatgg tctccggcag aaagagtaga
     3001 gaagactcta gtcccctcac cttccacccg ataaatgata cggcaagtat ttgtttcgtg
     3061 gtcgatatga ttcaagctaa tcggtcggcg taatagcaag tctgcacgcg gcacgagaat
     3121 atgcaagaac tgtcctggtt gctgcatttc ttttactaga tcacctttca atgtcatttc
     3181 aaaaatcttt ggtgccagct gtttttgctg aacgatttcc atcatttctt gtttcatctg
     3241 tcccctcctg caacggtttt tcgtcttttt gtcttaacat gtgctcgccg ttaaataggg
     3301 ttagttgaaa aagcacggga ttccatcact cgaacgatcg catctgctgt gtctaaagaa
     3361 gtaaacaatg gaataccatg ttcaactgct tctcttcgga tgatgaaacc gtctttagaa
     3421 gcatttcccc gatctttatc aatcgtattg acaaccactt gtgttttacc atctcttatt
     3481 aagtcgacta catttttttc tgctgcttcc tcagaaattt tcgctactgt ttcaacgacc
     3541 agaccatttt tttcgaaata agcagctgta tgtttcgttc ctatcagact atagccaatt
     3601 tctgtaaaac gtttagctaa ttctagtgct tcttcttttg tttcgtccgc aatcgtaaat
     3661 aatacggctc catattcagg caagtgtaat ccagaagctt cgaatgcctt gtacaaagct
     3721 ttgtctaatt gtctgtcaga tcccattact tctccagtcg atttcatttc aggccctaag
     3781 taagtatcaa ctttctgcaa tttcgtaaaa gaaaagacag gtgctttaac atgaacttct
     3841 tttgtttctg gataaagtcc gttttcataa ccaagtgtct ttagtttctc tcccaaaata
     3901 gctttcgtcg caatttgtgc cattggaatt cctgtcacct tacttaagaa tggaacagtc
     3961 cgacttgctc gaggattgac ttcgatcaca taaacctcat tttcatggat gacaaactgg
     4021 atattcatca tcccgacaca attcaaacct aatgctaagc ggatcgtgta atcttcgatc
     4081 gttttcttga tcgattcaga taatgtttga ggtggataca cagccattga atcccctgaa
     4141 tggacaccag cacgctcaat atgctccatg atccctggga tgagtactgt ttcaccgtca
     4201 caaatcgcat ccacttcgca ttcaccacca agcaagtaac ggtccactag tactggatgt
     4261 tctggagatg ctttcacagc atgttccatg taatcttcta aatctcgctg gttttcaacg
     4321 atttccatcg cacgtccgcc taatacatag cttggacgaa caagaactgg ataaccaatt
     4381 ctcgaagcga taaccacggc ttcttcttta cttgtagcag tatcccctaa aggttgagga
     4441 atatccaact ctttcagcgc ttgctcgaat aaatcacgat tttccgcacg atccaagtct
     4501 tcgatcgttg tccctaagat ttttacacca gcagcagtca aaggttcggc taagttgatt
     4561 gctgtctgac caccaaattg aacgattact cctatcggct gttctagctc gatcacattc
     4621 atcacatctt cgaaggtcaa tggctcaaaa tataatttat ccgaaataga aaagtcagtc
     4681 gaaactgttt caggattgct gttcatgatg attgcttcgt atcctgcctg ctggattgct
     4741 ttgacagaat gcacagtcgc atagtcaaat tcaacccctt gtccgatacg gataggacca
     4801 gatcccagta ctagaattga gggcttttct gatcgtgaac tttcattttc atattcatag
     4861 gaactataaa aataaggtgt ttttgattcg aattccgctg cacaagtgtc aaccatctta
     4921 tatacaggaa tcaaatcatt ttcttttctc atctggcgaa cggcttcttc gtttatttcc
     4981 cacagctccg ccactttttt gtctgtaaaa ccgttttgtt tcgctgtttt caatatcttg
     5041 tcatccaacg gctgctgttt caactgctgt tcgatttcta cgatatgcaa cagtttatct
     5101 aaaaagaata aatcgatttt tgtcaaatcc gctaattctt ctatggtgta ccctcttcta
     5161 atagcttcag ctacataaaa caggcgatca tcttgtgctc gaaccacttt ttggattagg
     5221 tcatcgtccg tgatgtctgc caactcagga agttcgatat ggtgtgtacc gatttctaaa
     5281 gaacgcacag ctttcaataa agactcttcg atattccgac cgatagccat cacttcacca
     5341 gttgctttca tctgcgtgcc taaaagccgt tctccgtttt caaacttatc aaacggccag
     5401 cgaggaattt tagctacaac ataatctaat gcaggttcaa attctgcaaa agtcgttcct
     5461 gtaacaggat ttttcatttc gtctaatgtt aatccaagcg ctattttagc agctagctta
     5521 gcgatcggat agccagtcgc cttacttgct aacgcagatg aacgagaaac acgcggattg
     5581 acttcaatca cataataatt aaaactatgt ggatcaagag cgagttggac attacagcct
     5641 ccctcgatct taagtgctcg aataatcgaa agagaagcat cacgaagcat ttgatattca
     5701 tggtcagaca atgtttgact aggggcaaat acgatagaat cacctgtatg gatacccacc
     5761 gggtcaaaat tttccatatt gcagacaacg attgcattat cagccgaatc tctcatcact
     5821 tcatattcaa tttctttaaa acctgcaata cttcgttcaa tcaaacattg ggtagcgggg
     5881 gaaagactta atccattttc tgatacgata cgtaattctt cttcattttc acacatacct
     5941 ccaccagttc cgcctaaagt aaaggctgga cgaacgatga caggatagcc gattttattt
     6001 gcaaaatcta cagcttcttc tactgtattg acgatttcag actcggggat tggctgattc
     6061 aattctttca tcaactgttt gaatagatca cgatcttctg cttgatcgat cgcagaaagc
     6121 tttgttccta gaagttctat gttcaattct tctaaaatac ctgatgctgc cagctccatt
     6181 gccatattga ggcctgtctg tccgcctaaa gttggaagga gagcatctgg acgctctttt
     6241 cgaagaatcc ttgatacaaa ttcaagtgtg atcggttcga tatatacttg atctgccact
     6301 tctttatccg tcatgatcgt cgcaggatta gaatttacta aaaccacttc atatccttct
     6361 tcttttaagg caaggcaggc ttgtgttcct gcgtagtcaa attctgcggc ttgaccaatg
     6421 atgatcggac cagatccaat caccataatt ttcttgatat ctgtacgttt cggcatctgt
     6481 ttactgctcc tttcctgcgt ccatcatttc cataaattca tcaaataaat gtagtgcatc
     6541 atgcggacca ggggcagcat ctggatgaaa ctgcacactg aaagcgggat aatctctgtg
     6601 tttgactcct tctactgttc cgtcatttac ttctacatga gttacgatga gtttttctgg
     6661 atcaatcgtg gcttcatcga ctgcatagcc atgattttga gaagtaaaat caattcttcc
     6721 ggtcgctact tctcgtaccg ggtgatttaa tcctcgatgt ccgaatttca ttttataggt
     6781 atctgcaccg ttggctaaag caaataactg gtgaccgaga caaataccaa aaatcggtac
     6841 ttttccttgg atttttttga tcatttcgat tgcttctggc acatcttttg gatcaccagg
     6901 tccattcgtc agcatgactc cgtctggtga tagcgctaaa atcgtctcgg catctgtgtt
     6961 atagggaagt actgtgaggt tacagtttct tttactcaat tctcgtaaaa tactatgttt
     7021 caagccgaag tcaatgacaa tgactttccg acctgttcct ggacttggat aaggtttgtt
     7081 agttgagact tgctgcactt gattggttgc tagaacggtt gctttcagct gatcaaatgc
     7141 atgttcgaag gaatctccag catcaactat actagctttc atcgttccga cttcacggat
     7201 tttgcgagta agggcgcgcg tatcgatacc tgatattcca ggaattcctt tttgtttcaa
     7261 aaatgaatct agcgtcattt ggtttcgcca gtttgaagct acacgtgcat gttcttttac
     7321 tactactcct ttgcatgttg gagaaatcga ttcataatca tctctattta ccccataatt
     7381 acccaccata ggataggtaa atgtaatgat ttgaccgtta aaactttgat cagtgatagt
     7441 ttcttgataa ccagtcatac ttgttgtaaa aacgatttct ccgaagacac tagcatctgc
     7501 accaaaagcc tttccttcga aaactgttcc atcttctaat ataagcaaac gttccatact
     7561 tttattctcc cttctgccac acaagttttc cgttgacaaa agtcatttgt gtttcgcctt
     7621 ttactttcca gccgataaaa ggtgtattct ccccttttga taaaaagtct tctttatcga
     7681 tcgtgcaagt ttgtgtaata tcaaaaactg ctacatcagc gggggcacca accgttagcg
     7741 ttcctgcatt caaaccaaaa atctcagccg gtttaactgc caaccaattg atcacttgtt
     7801 ccaacgtgaa tcgaccagtt tctacaaaat gagtatagat cagttgaaac gctgtttcgc
     7861 ttcctacgat accaaatggt gatttcatga aactttggct tttttcttct aatccatggg
     7921 gggcatggtc tgtagcgatg caatcgatcg taccgtcaag caagccttcg atcaaagctt
     7981 ctctgtcctc ccgtcctctt agcggcgggt tcattttcca aaagccgaaa tcttcgggaa
     8041 tatcttcgtc gatcaaaata agatgatggg gtgaaacttc tgctgttacg tggattcctg
     8101 ctttttttgc gtctctgatt acacggacac tttctttagt agaaacatgg caaacatggt
     8161 aatgaacacc agttgcttca gctagcaaca gatctctagc aatttgagaa gactcggtta
     8221 cgcttaagat cccgggaaga ccaagttctt ctgccttctt gccagcgtgc ataacgccgc
     8281 caaataataa agattcatct tctgtatggg ctactagggc tttattgttt tttgcagctt
     8341 ctttcatagc cagatacatc gtcccagctg tttggacacc tacaccgtca tttgtgaaag
     8401 caaaagcacc ttcttcaatc agtgcttctt gatcaactaa tttctcactt cgcagatttt
     8461 ctgtgattgg cgcatactgc aataccttca caactgcatc ttttctaatg atgtcatata
     8521 cttgtctcag cttttctgcc gtatcaggta caggatttaa attcggcatc gcgcatacag
     8581 tagtgaaacc gccacgagca gctgagcgag tcccagcttc gatcgtctcc ttgtatgtaa
     8641 atcctggctc tctcaaatga acatggacgt ccactaaacc aggtgtgatc aattgtccct
     8701 ttgcatcaaa cacttcatca aattcggctt ctgagaatcc tgtaccgatt gctttgattc
     8761 gcccatcttc tatccaaatt tctgctggtg tcgtcatatt ttttcgtgta ttgatctgac
     8821 cgtttttaat gagtgttttc attttttcat cctcctataa ttacgctttt ccatttagca
     8881 ctgcttctaa gattgccatt ctgacgaata ctccattgct catttgctga acgatccgcg
     8941 actgtaatcc ttccactaat gaatcagcta attctacatc acggttaact ggtgctgggt
     9001 gcataatgat tgcatgtttt tgcaacttct tcgcacgctc aactgttaac ccatgttcct
     9061 gatggtagcc ttcttttgag aaactttctt ttccatcatg gcgttcgtgt tgcacccgaa
     9121 gcatcatcat gacatcaact tgatctagta gttcatccaa tggcatataa tgtccgtaca
     9181 cttcgaattc atcgtcgtac cattctctag gacctgagaa gaatacttgt gcgcctagac
     9241 gttttaacat ctgcatattg gatttagcta cgcgagagtg agtgatatca cctacgatgg
     9301 caactttcag accttcaaaa tgaccaaatt cttcataaat ggtcatcaaa tcaagcaagc
     9361 attgggttgg atgctgtccg cttccgtctc ctccattgat aatcgaacac tggattgttt
     9421 tactttgtat cagttcgtca taatagttct catcaccatg acggataacc gctacgtcta
     9481 cgcctaatgc tgacatcgtc agtactgtat cgtatagtgt ttcccctttt tggacagagc
     9541 ttgtgcttgc ttcaaattca atcacttcta gccctagttt tttctccgct acatcgaagc
     9601 ttttatgtgt gcgagtacta ttttcgaaaa agaggtttgt agcaaagtac tggcgctttt
     9661 gcggagtcca attagcacct tgcttgaatt cctgccctct gcggatcaag cccataactt
     9721 cttgatcagt aagtgcctca acggtcaata aatgctttaa gctgatacgt tctgatttga
     9781 tgatcataat gtttcctcct tagttttctt cgcttcgtga agtttctggt aagataagat
     9841 ttaaaatgat gccaagtaca gttgctaatg ccattgccga caatgtaaat gtgcctactt
     9901 caaagacgag tccgccaatt cctactacta agataactga agcaatcaac agatttttct
     9961 ttttatcaaa atcgattttg ttctcgatta aaatcttcat tccacttgcg gcaattactc
    10021 caaataagat aaaactgatt cctgagatga ctggtccagg gatacttaaa atcaaagcgc
    10081 ttaattttcc tacgaatcct aaaatgactg caaatactgc agctccaccg atcacaaaca
    10141 cgctatggac acgtgtgata gccagaacac caatgttttc accgtaactt gttacaggag
    10201 gacctccgac aaaggctgcc acgatttgtg ctaatccatc acctgtcaac gtcttatgaa
    10261 gacccggttc ttcaaagaaa ttacgtttag ttagtttatt caataccata agatggccga
    10321 tatgctctgt catcgtcaca aaagcgatgg gtgccattgt tgtgattgct ccaagatata
    10381 gctttggttc atattgtaca aagggaatct caaagttagg aacagcaaac caggcagcgt
    10441 ctacgattgg cttagtatcc acgattccaa acagcaaggc tacgatgtat ccgctaataa
    10501 tacctagtaa aatcggaatc aatccgagaa aacctcttag ccacatgttg aagaaaattg
    10561 ttaatcctaa tgtgatcagc gctacagcaa tatacttgaa atcatacgtg ccctgattat
    10621 acatagcatt tgttgctgca tttgccgcaa gtcccaaccc gataaccatg atgactggac
    10681 cgacgacgat tggaggcaag attttatcca gccatgctga accgatcttt ttgatgatca
    10741 gcgcaacaat caaatagact aatccagttg tgactgctcc ttgtgcaatc gctggataac
    10801 catctgtttt catcaaaagc tgcatcgctg caatgaaagc gaagctgctt cctaagtacg
    10861 caggtatctt tcctttagta gtaaataagt acaccaatgt acctagtcct gaactgacta
    10921 gcgcaatacc tggatcgata cctactaaga tggggactaa cacggttgct ccgaacatcg
    10981 tgaacaagtg ttgtaaactt aatcctaccc aatgtcctgg ggttgggcga tcgtggatat
    11041 ctaacaccac gtcatcatta tgaaatcctt ttttctccga catgtttctc tctcctctaa
    11101 tcttcctctt tcaaaatcat gatgcgatct tgtccatcaa gctcttgcat ctctactaaa
    11161 atctcttccg cccgagatgt tggaatgttc ttcccaacat aatctgcacg gattggcaat
    11221 tctcgatgcc cacgatcgac caggactgct aatgaaattt ttcttggtct gccaaaatcc
    11281 attactgcgt ccattgctgc tcgaatcgtt cgtcctgtat acaaaacatc atcaatcaaa
    11341 ataacttctt ttccttctaa ggaaactggg atgtcagaag aatgaagttc cggttcttct
    11401 ggattttctt ttttgtcatc tcggtataaa gtgatatcca gttctcctac aggaatatcg
    11461 atatcttcta gctgcttcag gcgttcggca attcttgatg caatgtagat tcctcgtgtt
    11521 ttgataccta ccaatacgat atcttggata ctatggtttc tctcgataat ctcataagta
    11581 attcgagtca atgctcgctt catggttacc tgatctacta cttcttttgc ttgcattttc
    11641 attcccccta cttttttgca taaaaaaact cctgccctaa gaggacagga ggaatgttta
    11701 gactgagcgt atactgatac actgatagct atactatgtc aatgagcaat ctacttatgg
    11761 cgcaccatac gtatccttgc tcccaccctt cttagcctca cgggactaaa cttaaaggaa
    11821 ttctattaag ttttatgctt tcgttgttaa ttactatacc gttatctaaa ttaaaatgca
    11881 atgttttttc ttaatttttc taaagttttt tcaaaaactt ctggaagagg tgcttcaaag
    11941 atcatctttt cgtttgtagt cgggtgcgta aaacctaaca tttgcgcatg gagaaactga
    12001 ccattgcctt tcaaagtttt cttcggtccg tatactggat ctccagctaa cggataaccg
    12061 atatacttca tatgcacacg gatctggtgt gttcttccgg tttctagttt taattcaacc
    12121 agagtaaatc cttcgaaacg ttccaataca gtaaaatgtg tgacagccga cttcccatct
    12181 tctcttatcg cctgcatttt ccggttttcc ttcgaacggc cgattggcgc attgatggtt
    12241 cctttttcgt gaggaatctc tccatggact aaagcaacat atttgcgcaa agaggtctta
    12301 tcttttaatt gctgagctaa tgattcgtgt gcctggtcat ttttggcaac cattagcagt
    12361 ccggaagtat ctttgtcgat tcggtgaaca atacctggac gaataacgcc gttgatcgta
    12421 gaaagatcct tgatatggta catcagcgca ttgaccattg tgccatgcgt atgacctgct
    12481 gaaggatgaa cgaccattcc ttgaggtttg tttatcaccg ctacatcttc atcttcgtaa
    12541 acgatcgtca acgggatatt ttctgggacc acatctaatg tttctggttc ggggatcaag
    12601 acagtaatct cgtctccttg ttttactttg taatttgctt tgactggctg tccattgaca
    12661 gaaaccgctc catttttcag ccacagctga atttgcgaac gactgtaatc ggtgaataaa
    12721 ttagtcaata ctttatcgat cctaccagat tcatggtgaa tcgtcgtttt aagttctgtc
    12781 atttttttct ttgatccttt tcatctaaaa taatataaat taacatacaa atcactccac
    12841 atacaagtgt cacatctgct acgttaaaaa tcggaaagct cataaaatct gtctggaaca
    12901 tatctactac atagccaaga cgaatacggt cgataaaatt gccaatcgca ccagctagaa
    12961 tcaaactcaa tccaatcatg aaccagcgat tacctttttc acgattttta atcatcaatg
    13021 tgagaatcac aacgactgca atgagtgtca ctatagtaaa gaaccacatc ttcccttcta
    13081 gcatactcca tgcggctcca gtgtttcgaa tatgattcag tgacaaaata cctggtataa
    13141 gatttttcgt ctctccaagc gctagattat cagtgatcca ccatttgacc aactgatcca
    13201 ctcctactag caaagcgctg aatatccaat aaataattaa caaaaaatca atccttttct
    13261 atttgtttat aaatatcacg aggtcgtacg atcccgagca gattaccatc gggattccca
    13321 tctttagtga taagtacggc ttccaaagtc ccttgctcac gaaacatcgc ttcaacctcg
    13381 taaatatgtg ttttagctgg aacaaaatga taatttgtgt actttccgtt ttggatgatc
    13441 aaatccaacg ctcgtttatt tgttaaatca accggacctt tttggctttc ttttgctagc
    13501 caaaacccga tattacgtaa agtaatcaat gctacaaatt ttcctttatt gtataacgga
    13561 aattgtgaat accttttttc tgctactatc tttaacagtt caagtaacgg aagatcttgt
    13621 tcaaaacctg ttacacgttt tgcaaatcga ggcagcacgg tttcaggctg aatcagttct
    13681 tgttcgatca gctggattcg cttgactgtc caatcatttg gctctgcaat gacaaaatcg
    13741 actgcgatcc gatcatggac gatcgcattc cttaactgag caagctgtaa aagatcatct
    13801 tcgtattttc tgatcattaa ttgttttctt tgagctaggc gtctgacaag ttcggtaaat
    13861 cccatatttc gtgcatttcc catctcgtct ctcatccatt tttcgattcg attgaaactg
    13921 attaaaaaaa gatcagagtt tgccataatt ttcctccttg atcaaatatg cccggttttt
    13981 tatatagaaa aagaacgcaa tcaacacaat aactgcaaaa aaactgaaaa ataaattttg
    14041 taggaaaacg gcccccaacc cagttgctag aaataaaaac ggatagattt gaagaatcaa
    14101 gtaattcatt gtgatcacat aagcaggctt tgcttctttc actgtataaa cggggctttt
    14161 tagccaataa aaggtgacct gcatcgtaaa gggcacttcc agatcaagcg ttatcttttg
    14221 ttcatgactg agaacactgc gtttttcatt gttgattctt agctgaatcg agctacccat
    14281 accgtaaaaa ccggtttttc ttataaatgt cgttttcatc ataccctcct attatgcttt
    14341 agtatagctc aaaatacaat aaagaaaaag aatgatccta gtgtaaacaa aattaattcc
    14401 ctatcgtcta aaaaaagaag ctgtagtcaa aatacagctc cttcgatggt agattataat
    14461 caagcttaaa acaatcccaa cacttctccg tcatttgtca cgtccatatt caatgcagca
    14521 ggttttttag gcaaaccagg catcgtcata acatctccag tcaaggctac taaaaatcct
    14581 gcccctaatt ttgggatgat ttcgcggatc gtgatggtga aatcttccgg tgcacctagc
    14641 agtgaaggat catcagaaaa tgaatactgt gtttttgcca tacaaatcgg cagtttgtcc
    14701 catccattct tcgtaaactc ttctatttgt ttttgcgctt ttttgctgaa aacaacacca
    14761 tttcctccgt aaattttctt gacgattgtt tctgtctttt cttgtatcgt ttcttctagg
    14821 cggtataatg gcttgtaatt agcttctttt ctatcaatca atcgtactac agcttttgct
    14881 aaatcaacgc cgccttctgc tccatctgcc catacactcg ctctcttggc aagaatcccc
    14941 tgattttcac acaagtgctc caacagtgtg agttcattgt ctgtatctgt tacgaattca
    15001 ttgattgcga cgatcacagg tagctgatat tgttccatat tacgtatatg gcgctttaaa
    15061 tttgcaaaac ctactttcaa agcatcaaga ttctcatttt taagttcatc ttttttcatt
    15121 ccgccatgca ttttcaatgc acggacagtg gcgacaatca ccacagcatc cggcgctttt
    15181 ttcaagttag gtactttgat atctaaaaat ttctctccgc caagatctgc accaaatcct
    15241 gcctctgtga caacgtaatc tgctaatctc aatgctgtct tggtagctaa aatactattg
    15301 cagccatgag cgatattggc aaacggtccc ccgtggacaa aagctggtgt tccgtaaatt
    15361 gtttgtacaa gattcggctt gattgcatct ttcagcagta atgccagagc gccttctgct
    15421 ttcaagtctc cagcagtcac aggctgacgg tcaaatgtat agccgacaac gatccttgca
    15481 agacgagcct ttaaatcttc aaggtctgcc gcaagacaca agatagccat gatttcgctg
    15541 gcaactgtga tatcaaaccc atcttctctt ggaacacctt ggattggccc acccaagcca
    15601 acaattactt tacgcagttc acgatcattg agatcgacta cccgtttcca aatcactcgg
    15661 cgggcatcga tgttcaattc atttccttga tgaatatgat tatctaaaag agcagataat
    15721 gcattattag ctgttgtgat agcatgcatg tcaccagtaa aatgcaagtt gatatcttcc
    15781 ataggcaata cctgtgcata tcctccgcct gcagcaccac ctttgattcc catcacagga
    15841 ccaagtgagg gttctcgcaa tgcaataaca gattttttat tgattttatt gagcgcgtct
    15901 cctaacccta cagtgatcgt tgactttcct tcgccagctg gtgttggatt gatggaagta
    15961 actaagatca aatgcccctc agcgttatcc tttgatcgat tgattccaga aaaattgatt
    16021 ttcgccttat atttcccata cagttccaaa tcgtcttcct taagatctaa tttactagcc
    16081 acttcaacaa tcggttctag ctctgtttct tgtgaaattt gcagatcatt cttcatttga
    16141 ttaccccttt tcacgaactt tattcaacaa aatatcttc
//
LOCUS       DACCXW010000062        16069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCXW010000062 DACCXW010000000
VERSION     DACCXW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.062"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1162
                     /locus_tag="IVY71_001674"
     CDS             <1..1162
                     /locus_tag="IVY71_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7985728.1"
                     /translation="KLDDSPSYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYK
                     LTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQL
                     RVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVD
                     ELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQS
                     GISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1545..2273)
                     /locus_tag="IVY71_001675"
     CDS             complement(1545..2273)
                     /locus_tag="IVY71_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7985729.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2251..3825)
                     /locus_tag="IVY71_001676"
     CDS             complement(2251..3825)
                     /locus_tag="IVY71_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7985730.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            4054..4992
                     /locus_tag="IVY71_001677"
     CDS             4054..4992
                     /locus_tag="IVY71_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7985731.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            5004..5915
                     /locus_tag="IVY71_001678"
     CDS             5004..5915
                     /locus_tag="IVY71_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7985732.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            5939..7411
                     /locus_tag="IVY71_001679"
     CDS             5939..7411
                     /locus_tag="IVY71_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7985733.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7462..8073
                     /locus_tag="IVY71_001680"
     CDS             7462..8073
                     /locus_tag="IVY71_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7985734.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            8103..10016
                     /locus_tag="IVY71_001681"
     CDS             8103..10016
                     /locus_tag="IVY71_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP7985735.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            10144..13125
                     /locus_tag="IVY71_001682"
     CDS             10144..13125
                     /locus_tag="IVY71_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP7985736.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            13122..14783
                     /locus_tag="IVY71_001683"
     CDS             13122..14783
                     /locus_tag="IVY71_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP7985737.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14823..15755
                     /locus_tag="IVY71_001684"
     CDS             14823..15755
                     /locus_tag="IVY71_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP7985738.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         5206 a         2604 c         3217 g         5042 t
ORIGIN      
        1 aaaattggat gactctcctt cctattgtga acactgtcga tcggttcgta ttatccgcaa
       61 cggttcctat acgacacgca ctcagatatt gaaagtcaaa gaaaagttaa ctattttaga
      121 attaaaacgt acccgttttc tctgccatga ttgtggaagg accttttctg cgaaaacgga
      181 cttagttgat gaacatcatc aactaacaaa ggaactgaag caggcaattc tcatggaact
      241 gtacgaaaat caatcacgta aactaatcgc taagaagtac ttcgtctcgg atgggacggt
      301 gacacggatc ttgcgtgaag caacgaagca ttaccagtca cgtatgaact tccttccgac
      361 ggtgttgtgt atggatgagt tcaaatcaat gaagtctgtt tctggttcca tgagttttat
      421 ttgtgttgat gggacaaccc atcagctatt tacgatcctt gaagatcgca gactttacaa
      481 actaactcaa tacttcctgc actttcctag aaaagcccgt ttaaaggtca agtacctagt
      541 gatggacatg aatgccagct attgtcagtt gcttaaaaca gtctttccaa atgcggagat
      601 cgtaactgac cgctttcata ttgtccaaca catcaatcgc agcttcaatc aattacgtgt
      661 ccagatcatg aatcgttttc gaacctcaca ctctgaaaat cagaagaagt accgtcggct
      721 caagcgatat tggaaactct tactcaaaga ttccacgaca tgggagccac tcaaacggca
      781 ttatcatcga ctgtttaaac gaccaattag tcaaacagaa atcgttgatg agctgttaag
      841 ctacaacgaa gaattgcgaa ctgcgtatca tttttgtcaa ttattacgct attatttcgt
      901 taaacgaggt acggaaggct ttcaagaaac ccttaaaaca atttcatcta cgttacctca
      961 gtcgtttaaa aagaaattca cgatcttcca ttgctaccaa tcgggtattt caaatgcctt
     1021 taaggtgtcc cactcaaacg gcgtcactga ggggctgaat aacaagatca agctgattaa
     1081 acgaattgcg tttggttatc gcaactttta taactttcgg gcacggatct atttacaaca
     1141 aggtctgatc tttgaaaact aaaaaaatat gcggcgcaca gaacgcgccg catatcggac
     1201 taagttttat tcaaatttgt cttcaccaac acgatttgac gaagagccta tttaaataac
     1261 tacatccatt tattctatct tctaaaatag cctttttcat ttcagttaaa tagttcagcg
     1321 ccatttttgc actcgacttg catagtagtt ttttccatat tcctcaacct cagcgggggt
     1381 tgaagacata aggaaaatcc tatgcgttat gctcaactgg atctgattaa atattctaat
     1441 gtatttttct ctaccatcat taaatggtta atagccaaaa atcaccactt ttcaatggct
     1501 ccttgttgta taagtataac tagaagatga acacgtctga gaaattatag aagcttttta
     1561 cggtaattac tggggctgtg tccagtagtt tttttgaaga gaacagaaaa atagctcacg
     1621 tgatctattc ctactttttg actaatttca tcaatagata agaatgtgtc tttcagtaga
     1681 gcacttgctc ttttgattcg aaagtctagc aagtaatcat gaaaggaata acctgtttct
     1741 tttttgaagg cccgtcctaa ataagcggga tggtaatgga attgtgtagc tacatctttg
     1801 agcgtaagct tttcactata attgttttga acgtaatcaa tcacttgttg aattgactca
     1861 ttaaaagaat catcagttag agagttttga ttagctgctg atttacgcat tttttcttct
     1921 tgtactaact taactaaata aggaaaatca atgggtttcg ttatgtaact tttaatagat
     1981 aaatcaatcc ccttttttgc atactgaaaa ttttggtggg cactaatgac aatactagta
     2041 aagtagcgat tggatgtttg ttgcaaagat tcgattaatt cgagtccaga aattttcccc
     2101 atcgtaatat ctgtgataag taaatcaaag cgctggtttt gcaggttttt aaatgcttcg
     2161 tctccgtctg tgtagcagtg atcgataatg atgtcgggga ctttcttcaa aagataatgc
     2221 cttatgcctt ctacaattgt acaatcatcg tcaacaattg caattctcaa catgcatatt
     2281 cccccctttt tgtattttga tagtaatcgt tgttccgacg tgtagttggc tttctataac
     2341 aaaacgtgta tctccaggat agagatgctt tatacgtcga aaagaatttg agatgccgat
     2401 attctcacta taaggaatat catcattaag tttttgttga atatgctcca gcttgtcttg
     2461 gtccattcct ttcccatttt caacgatttt taacacataa tggttggttg tttcagttgc
     2521 atagacagtc aaacgatttt ccttttttga tgaatcgaag ccgtgtttaa tataattttc
     2581 aacaaatggc tgtaaagaaa gttttggtaa cattaattga tcaatatgat caatcagctg
     2641 atagtcaaag ttatgtcgat aacgacgttg atgtagttgc aaggagaggt cgatattaat
     2701 taattcctct ttcaaatcaa ttatgtcggg ctgatatgta ttattgcgat acaacttgct
     2761 caataaatat atactgtcgc tgatttcttc gtcatcattc aatatggcag tcattcgaat
     2821 aaattccaaa ttattaaata gaaaatgagg ctcgatttga tattgaagtg ctgcatactt
     2881 tgcttcttgc ttcaaccaat tattttcata aagtgttttt tgatatgtag cattatctaa
     2941 ataaaactga ttgatatgca tagcgatttc tttcaaatca ccgactttgt tttcgatatt
     3001 gattgacttg tagtctcctg tagatgaatg atttaaggaa gtaatgattt cctgatactg
     3061 ctggatatag ttacgaaaac ttctatttaa taattgatga atagcaagaa taaacaaact
     3121 cattaataag aaaatcatga ttgccagacc taataaggtt tgtgacgaat aatttggatt
     3181 ttcatatact tgtaatgtgt ggttaccgat agctatctgt tgcgtttttt tccatttaga
     3241 aggcgagatg tcttcagata aattgacgga atcaatacca ttagacaaag aataattgta
     3301 atgtgttgga aagagactct tttttagcgc gttgatcgaa acgtctagaa tcaatgtagc
     3361 atcgggcatg tcctcactaa tgaaacttaa tgaacgaata aaagaaattt tttcgttttt
     3421 tgtaggtgcg taggcactta agtatttttc gtaatctaat gagatagata tatttttaac
     3481 ttcatcatct tgcacaagtt tttttaagtt atctactaaa taggtagggg agcgatcctt
     3541 tgacaaaata tcttccatgt attcagcagg tgatgagagg tagtagtcat taaggttttc
     3601 ttggaaatca taattttgat agatctctgt cacgataccg gagagaatac gattatatgt
     3661 ttccatatag tgggtgattt gctgaatgtt ttgcaggttt tgattttttt ctttttcaat
     3721 cacattgata taggaaaaga acaatgtgaa acaggcaagt aaagtaataa aaaaaatcaa
     3781 tatgtatgta aaacgcataa taacttttga taaatcgttt ttcattcaaa gtcctcattt
     3841 tgataatctc taattttatg aatcattata gcacatatta caagtgatgt aaacgctttg
     3901 caaacagggc tacttatgat tgtgaataca gtttccgttt aaaaactatt ctattgtaga
     3961 ggtttaataa actaaaaata atgtataaaa aattaatgta agcgattaac caagtgggta
     4021 taatcaagga aacgctgaga gaggagagac aaaatgaagc taaagaaagg gttattagga
     4081 aatctaaaaa aaaaccgagc attgtttatt atgcttttac caggtgcgtt atggttattt
     4141 atattttgct atataccaat gtttggagtt gtaatcgctt ttaaagattt cagaatttca
     4201 tcaaggggat tctttacaag tttgttcaac agtccttggg ttggctggga aaatttcaaa
     4261 tatctctttg cttcgcaaga tgcttggatc attactcgca atacggttgt ttataacgtt
     4321 gtaggtattt tgctaagtac tttttgtaca gtgtcattag caatcattct gagccaaatg
     4381 gcgaacaaac ggttattgaa aaagatgcag acatttatga tttttcctaa ttttttatca
     4441 tgggtagtca tcagttactt tgtttacgca tttttgagta cggataaagg attaattaat
     4501 attttactaa gcaattttgg tgtggaaccc ataaattggt atagtgaacc gaagtactgg
     4561 ccttttatta ttgtttttac gagtttatgg aaaggcgtag gctatggatc catcatttat
     4621 tacgcgtcaa ttatggggat cgatactagt tattatgaag ctgctttagt tgatggtgcc
     4681 agtaaatggc aacagataag gaatgtagtc ttgccacatt taagatcgat tattgtcatt
     4741 atgttgattt tgtctgttgg tagtattttt cgagctgatt ttggattgtt ttatcaattg
     4801 ccaagaaatt cgggggctct ttatgatgtg acgaatgtat tagatactta tgtctaccgt
     4861 ggattaatga atagcggaga tatcggaatg tctacggcag cgggattata tcagtcatca
     4921 gttggctttg tcttgatttt gattactaat aagatagtca acaagttaga tcctagttca
     4981 gcactatttt aggaggaatc aaaatgatat caaaattaaa acggcagatg tacttttcgc
     5041 tgccagtcga acggtttttc acaatattac taatcttatt tactcttagc tgtatttttc
     5101 cctttatttt cgtaatcgtc atttcattaa cagatgaaaa agcattacaa ctcaacggtt
     5161 acgaattatt gccggctcaa tggagcactg atgcatatca atacttaata caagatggcg
     5221 gacagttact tcggtcatta ggcgttacaa ttatgattac agtgattgga acgttgatta
     5281 ctgtttttat gacgggaacc tatgcttatg ttttatcaag agcctctttc ccttaccgga
     5341 agttctttac tttttatctt ttttttacca tgttatttgc gggagggatg gttccttctt
     5401 acttagtgat gacgaagatg cttggcttaa aaaatacaat ttgggctttg attctacctc
     5461 ttgcttttag cccatacaat gtcattattt taaggacatt ctttaagaag tcgattccag
     5521 aatcgattat cgaatccgct aaaatggatg gctgtagtga gtttcgagtt tttttccaaa
     5581 tcgttttgcc tttagcgatc ccaggggttg caacaatagg tttattttca agcttaggtt
     5641 attggaatga ctggttcaac gcattgctgt atattgacac caacaaattg attccattgc
     5701 aatatttgtt aatgaagatt caaaattcaa tggaattctt agcaaataat aatgacatta
     5761 cattagcgca gcaacaagca attcaaaata gccttcctca agaatcaaca cgaatggcaa
     5821 tggtcgtagt agctacttta ccgattgcaa ttgtttatcc gttcttccaa aaatactttg
     5881 ttcaaggatt gacgatcggt ggagtgaaag aataaaaaag aataggagat gttaaaagat
     5941 gaaaaagaac atgcagaaat atttgattgg tggtgcttct gtactaaccc ttggcctatt
     6001 aggtgcttgt ggtagcagta atggcgaagc aaataatgaa acaggaacca caaaagttac
     6061 ttggtatcag gttggagaag aacccaaaga ttttgatgag gtcatgacaa aagtcaacga
     6121 aaaattaaag gaagaaatca atgtagagtt ggatatgcgg ctgattccgg acggggatta
     6181 tcagcaaaag cttggtgtga tgatcaattc aggagaagaa tatgatattt gtttcgtcaa
     6241 tggcacagat tatgtgaatt atgggaacaa aggtgctttc attagtctca acgatcattt
     6301 ggataaagag ttgaaagatt atgcagctga attgaatgaa ggatttattg aaggtggggc
     6361 aattaatggc gaaacttatg gattgccagt caataacgat attgccacaa cggaatattg
     6421 gacctttaat aaagatttgg tagatcaata ccaattggat gtagagaatg ttgaaggtgt
     6481 agaaacattg gctccagtat tagaaaagtt tgcagaaaaa aatactgata cgaatattac
     6541 gccaatcggt atgacccgta ccagtgtctg tattccttat gacaatatta tcggtggtgg
     6601 tgtaccgcta gcggttgcat ttgaaggcga tacacaaaca gttgttaata cttatgagac
     6661 tccagaaatg atgacttatt tgaaggctat gcatgagttt tataaaaaag gatatatttt
     6721 gaaagatgca gccactgtta cgacagatcc atttcgaatt gatagtaaaa actggttttt
     6781 aggtcgtaac tcaagtggtt atcaaccatt agcacaaaaa aaattagaag ctctagcggg
     6841 acatggatta attttaaaac cagctggaaa agcttttaaa acgactggat ttgcaagagt
     6901 ggcaatgcat gcggtatctg cttcaagtaa acatccggaa gcagccttga aattgttgaa
     6961 ttatgtgaat accaatcaag aagtagcgga tttaatggtt tatggaattg aaggaaaaca
     7021 ctatgatcgc aatgaagatg gttcgattac tttaacaaac tctgatgatt attatcaagg
     7081 cgcttgggca ttggtaaata cttatctagt ttcaaaaact tcaccgatta cagaagaaag
     7141 taaaaaacta gaagaagaaa gtaaaaagtt tcaaaatgaa gcaccgatgt ctcctatttt
     7201 aggtttctct tttgacgctt ctgattttcg aacagaaatt gcaagtgtac aaaatatcgt
     7261 gtcacaatat gaagtacttt taagctcggg aacagttgac cctgaacaat atgtaccaga
     7321 gttgttagaa aaactagaaa aagctggatt ggataaaatt caggcatctg ctcaagaaca
     7381 atacgatgaa tggcgaaaat ctaacaaata attttttact aaatgaaaag aatagactaa
     7441 gcagttagtg gggggattca aatgaacgaa aacaaatgga ccaaaggatt taattgggtt
     7501 tttcaattgt tgttactaaa ctttacttat tttatactaa tttgtttagg atttggaatt
     7561 ttaggttttt tccctgctac gatagctttg attgctggcg ttgtgaatcg aaaaaaatat
     7621 ggcttgcaag agtctgtgaa gttcatgagt aaaatctata agaaaaattt tgttagatat
     7681 aatctatatt gctggtcagc aactttgttg ttggccggca atattactac gatctggctt
     7741 tgttggcagc aaagcgcacc attgttttat ttattaagta cattattatc ggtaattaca
     7801 ctgttcctag tctttgcaat gttgaattgg gtcgtgatca aaagtcttgg gttgtggatg
     7861 atgggagacc gactgattga ctatttgctt ttcacagcaa cgatgttgcc aacggtagtc
     7921 atgcaaggct tggtgcttgg aatttatttc cgaatattga tgattttgcc tgtgattttt
     7981 ctgtttggcg gaacagctgt aacggtaata ttgattagta aattaactga aagagcgtta
     8041 gcaaagttgc cttgtcagat acaatcgcac taattttttc acaaaaagga gtatttcttt
     8101 ctatgaagtt aactgataaa caagtagcta atatagcctt tatccagcaa cttgagattc
     8161 cattgacaca aaaagatgaa gaacaaatag acttgatttt tactgacaca aaggctaatg
     8221 tgcgacaaat caagattact aaaaatgagc agcaacgaat ttgcttgtct tattcacatg
     8281 aagacttgct gtatcgagga ttgtttatct gccttccacg ttttttagct caaggaggag
     8341 agatgccata cattgaagaa acctcgatca ttgagaccag ttattcatta gatgcttctc
     8401 gtaatggcgt gatgaataaa gagactctaa aaaaattcat ttcttactta gcgttgatgg
     8461 ggtacaacac attgtatatt tacacagaag atacctatga agtagaagac cttccatatt
     8521 ttggttacct tcgcggagcg tatacaaaag aagaagtgaa tgaaattgtt gaatttgctt
     8581 ctggttttgg catagaagta gttccatcga tccagaccct tgctcacttg acccaatttt
     8641 tgaagtggta tgctcaacaa gaactgatgg atgatcaaaa tactctactg atcggtgaag
     8701 aaaaggttta tgcggcaatc gaaaagatga ttattgcctg caaagaaatg tatcagacag
     8761 agcgtattca tttgggaatg gatgaagcct atcaagcagg tctgggacga tatttaagcc
     8821 tgtatgggta ttgtgatcgg acaactttaa tgattcaaca tatggaccgt gtgttatcaa
     8881 ttgttgataa atataagatg aaaccaatga tttggagtga tttcatttat aagctattag
     8941 atgaaaccgg taaagatagg tattacaatc ctgattttaa actcaaagca ttagcagcta
     9001 aaggattacc agcaggatta acctatgttc attgggacta tggttcagat gaagtttcct
     9061 cctatgagaa agtaattgat aaccatttga aattttgtga cattgatcat tacgttttag
     9121 ctagtggtgc ccatatctgg gggagactag ccccgaatca tgggaaaaca gaagcaattg
     9181 tttctgcagc tgtaaaagct tctaaaaata aaggtgtacg ttctgtgatg ttaactactt
     9241 ggggagatga agggcaggaa acagagcatt ggcattcctt aattggggca ttaaaattag
     9301 cagattatgt ctattctgaa gaagagtgga atgattttga ggaaatctac gatcgtttat
     9361 taggaacagg tagttatcaa ttaatgtccg aattacgata tttcgatgaa gtggatacca
     9421 ttacaaataa caacttacaa atggcttccg tgagtaaact gctactttgg caagatccgt
     9481 tagttggtat atatgacttt catgtagccg ctcataacaa aattgccact agaacaattg
     9541 gtacttatta tcatgagtta gctcagaaat tagatatgaa taaaaattgg aatccaaaac
     9601 aaccaatttt aacaactatt cgtcaacgat atatactttt agctgaaatt ttagcacaaa
     9661 aggcagaact aggcatctat ttacaacact gctgtctggt agaagacctt gcaggattaa
     9721 tggaattagc gaatcggtgt gaagcactag ccaatcgagt aaatgaattg gcatctattc
     9781 atgaaataat ttggtatcaa acatacaaaa cctatggttg ggaagtatta gaacaacgat
     9841 atgctgcatt agctcatcgt ctgaaaaccg ttgcgcgtcg tattaaaaat catcttcagg
     9901 ggcaagatgc cctagaggag cttttggaag aacgattacc gttttgtcaa tatcaagtgc
     9961 cgcttgaaat ttctggcttt aattatcggc aaagctcggt gtcaggctac aattaacgaa
    10021 ggaatcttcc cgttgaacgg accacttgct catctaatgg gaaagtggtt cgttttctat
    10081 tcataagtag cttaccacta actcttttat taagaagaga aaaaaagtag gaagtgagaa
    10141 aatatgttat cggactatta tcaaaatctg tcagttataa gtgtcaatac agaaccatta
    10201 cgtaattatt atattcctta tgcaagtaaa gaagaagcaa aacaagagtt agaaagggaa
    10261 aaaacgagcc agtttatcga tctcaatggt acatgggatt ttcagtatta tccttctgtc
    10321 caacagttac cggaaaattt ttttcaagag gcctttactg aaagctatga tagaatttct
    10381 gttcctggag tttggcaaac agagggattt gacttacctc agtatatcaa tgtatcttat
    10441 ccaattcctt ttgatccgcc ttatgtacct aatgataccc cttgtggtgt atatcggcgt
    10501 cagtttcaaa ttgagcaaac agatgcacgc ttttacttaa atctagaagg agcagatagt
    10561 aattgttttg tttggataaa tagaaacttt gtaggctatg accaagtgag tcatagtaca
    10621 tctgaatttg atgtgaccaa gtatttacag gttggggtta atgaaattca aattctagtg
    10681 atgaagtggt gtgatggtac ctactttgaa gatcaggata agtggcgaat gagcggtcta
    10741 tttcgtccag tgtatcttct tcaacgacca actatgcatc tgacaagtta tcaactacgg
    10801 acgttgactg atcctaaaaa aggaagcgga acattaatgg tttcttttca acgatttact
    10861 cctattgaag tatcgttacc tatttcttac gaattattcc atcctgatgg acgtctcata
    10921 gcgaaagggg agtgttactc ggaacaacaa ataaatttgg atgttgaaga ttgccttttt
    10981 tggacagcag aaacgcctaa attatataca ttacttttaa catgtaatgg tgaatatttt
    11041 aaagaaaaag ttggctttcg agcactgacg atcaagaatc gtaagttatt gctgaacgga
    11101 acacctatta aattatatgg agtcaactat catgacgtga atcagcaagg cacgccagtg
    11161 atgacccgag agcagttacg tgcagatttg ttattaatga aaaaattaaa tgtgaatact
    11221 gtacgaacgg cacattaccc taaatcacca gatttttatg aattggcgga tgagattggt
    11281 ctgtatgtaa tcaatgaagc agatttggag tgtcatggtg tggttaatct atatgatact
    11341 gaagcgaatt atcaactaat tgctaatgat ccgacattcg aaaagccgat tatggaccga
    11401 gtgatccgtt ctattcttcc ttatcaaaat cgaagctcta tcattatgtg gtctatgggg
    11461 aatgaatctg gttatggagt aaattttgaa aaagctcaag ctttagcacg gaaattggat
    11521 gattcacggt tgattcataa tgaacgctcg attgaaccag ttattgacca agtgaatgat
    11581 tttagtaact tggatgttat tagtcaaatg tatcccccac tggaagatgt tcatcgtcat
    11641 tgtcaaagaa gtgatactga aaaacctttt tttatgtgtg aatatagcca cgctatgggc
    11701 aacggtcctg gagatcttaa aacgtacttc gatttgattc aacagtatga ttcctttctt
    11761 ggcggttgtg tctgggagtg gactgatcat gcaatcaata gaggaacttt agaaacgcca
    11821 tcttatttat acggtggtga ttttggtgaa aaatatcacg acggcaattt ttgcgtagat
    11881 ggtttattga ccccagatcg gcaattaaca gtaggtgcat tggagtaccg taatattcat
    11941 tcgcctattc aactgattgc tgtagatgag gagctgcaag aagtaacctt gagcaatata
    12001 actgctttta cagccggttc agcatttaac ctttctcttg aatggtatat agatggaaaa
    12061 aaaacagttt atgaagtaat gaatcctttc atacttgagc ctggcaaaag ttgttcattg
    12121 cccatcacag tacctacaac gattcatggt gctcatacgg ttgctgtttc gctatctgtt
    12181 tataaaaatg atgagcttta tggacagcaa cagctctttc ttaaaaaaga aaaaatgaga
    12241 ttgccaagaa ctgctcatca taatgatgca ccacaaattt cagtaaaagg cacagagctg
    12301 ataatcaaag gagaatcctt tatttatata tatcaattag caaaagctgc gttcacttct
    12361 gtaaagatca atcaaaagga acttttggaa cgtcctactc aattttcaat ttggcgtgca
    12421 ccaacggata atgatcgtaa cttagtaaac aaatggaaag cagctggata tgctgaaatg
    12481 tctttagaaa tcagaaaaaa agactggaaa ttcgtagatg atcatctcct gatcgaaacg
    12541 tcttatcgag gagttattcc aggattacag ccaattttta caatgcacat acagttgaaa
    12601 ataaatggtg caggcgtaat caaccaaaaa gtatcagtgg agaaaaatcc gatttttcca
    12661 gatttccctc gctttgggat ggactggccg ttgaatgata ctttttcgaa ggtttcttat
    12721 tgtggctatg ggccgtatga aagttatcct gataagcact atgctgcgat gtttggtatt
    12781 tatgagatgg agattgagga atttgatcag ccatatatca aaccacaaga atatggtaac
    12841 cgctggcaat gtgattgggt atcaatcaaa aatcatcaag gatctaccat gacggtttat
    12901 ggaaactcta atttttctgc tctgccttat agtcaagctc aattaaccga aaagtcacac
    12961 cattatgaat taataaaaga aaatagaact tggttcaatt taagttataa acaaaatggt
    13021 atcggctcac acagttgcgg tccttattta gaagatcgtt tccgttttat ggaagatact
    13081 tttgactgga atatagtgat tactttcaaa ggagaaaata gatgaaagaa acaaagatca
    13141 atcaagtcaa aaaaaaatta tctcgttatc aagaagttgt ttatcgaaac cattgcttaa
    13201 aatggtcaaa aaataagctg aatgttcgtt ttaatggatt attttatcgt ttcgaacagt
    13261 tagaagaagt ctggaaaccg gtaacgaatt tccaatcatg tcctgtacag ctcataaatg
    13321 atccaaaaga aacatacgat ttatgtataa cacaccttta taaccagcat ttgaaaaatg
    13381 acggatttta tttaaaggtt actaatgaca atcaacttac gattgaatca gcaaaccttc
    13441 gagggtttca atatgctatg gaagccttat tagagagttt tttttcggta ggagatcaat
    13501 tattattacc ggaattgatt ctgaaacatg aaccatcagt gaaaattcga ggaatcattg
    13561 aagggtttta tggtatcccc tggacacatg aaatgcgcct ctctcttttt tcatatatga
    13621 aagataacca attgaatact tttatgtatg cccctaaaga tgatgaatta ttacgtaaaa
    13681 aatggcgtga actctacgat gcgcaagaat tagacaaatt taaggagctt ttatctgctg
    13741 ctgaggctag caatatcgat ttttggtact taattagtcc tggaaatgat attgatatta
    13801 cgtgtgaaga agacattcaa gttcttctga aaaagttaga acaactgatt gagcttggcg
    13861 tatttcaatt tggtctatta atggatgata ttgactatca attaaaaggt gctgctaaac
    13921 gccgttttcg agagccagca ttcgctcatg cctatttagt gaatcgagta gaggaatatc
    13981 ttagcactcg gttgtccaat catgaattag tcatttgtcc tacagaatat gataatcgtc
    14041 atggttctcg ctatttagca acgttaagtc aagaaatacc agaacaatta ccgttctttt
    14101 ggactggccc aagcacgtta gcggcgtcaa tttctacaga agagctgcag gaaatggctt
    14161 ctgtctatca acggccgatg ctgatttggg ataatattcc ggtaaatgat tatttagagg
    14221 ataaagaact attgttcatg agtccatatg agaatcgaac accgaatctt tctaaggaga
    14281 gataccaggt tacaggagta gtttcgaatc caatggctca attagaagct tcaaagttta
    14341 cgatcaatag tatggctaat tacttatgga actgtgagcg ttttgatcct ttggaaactt
    14401 ggacgtcagt cgttgaaaaa gtagttggaa gtaaactaca accagcttat cttaccttaa
    14461 ccaatgcttt tccaaatcat tacacaagca gtctgctttc agatgaagaa ttactgctag
    14521 ccaaagaccc agaatgggtc acaaaagaaa tggctgcttt agtccaagca gtaaaatgga
    14581 tccagcaaga acaacataca tctagattga gaacagaatt ggagccttgg ttacaagcaa
    14641 tcacagaaag ttggactttc tggcaagaat ggcaattaaa aaaagatcca aaagaagcag
    14701 aagaatggtt ggctaagaaa aaaacacacc gcataggacg tgatttagta actgcccatt
    14761 tagagcaaat tatcaaaagt tagatatctt aaaaaataaa aagcaagaaa aaggagaaca
    14821 aaatggtcaa gagattaatt agtgcaaact acagtgatgt caagaaaatg acgtcaaaag
    14881 agttgaagca atccatcaaa gcgagtgaag gacgggtgat tgtttgtgaa acggttgcta
    14941 atactcttcc acaatctgga gaaattacga atgcagaagt tgcgtcagca tttggtgcag
    15001 atttgattct tttaaatggt ttcgattgtt atcgtcctgc agtttttgga ctaccaaatg
    15061 tgacgattga gaaggttttt gatctacagc aaacgattga aaacccgatt cctaaattaa
    15121 aagaactagt cggtcgtcct gtgggaatta atttagaacc cattgcccct aaaacaaata
    15181 tgatgagtga gaaagtagaa atcagccaag gaagagcaag ctctgttgaa acgatacaaa
    15241 aagcagaaga actaggagtc gattttattt gctttaccgg taatccaggc actggagtaa
    15301 ccaatttaga aattactaaa tccgtaaaaa tggcaaaaaa gtatttttcg ggattgatta
    15361 ttgctgggaa aatgcatagc gccggctcag aagagccagt tgtgaataaa gaggcgatta
    15421 tttcttatgc ggaagcaggc gcagatattt tactagttcc ttcagtaggg tctatcccag
    15481 gctttttaga gcaagaccta attgaagcgg ttcggttggc aaaaaaatat gatttgttaa
    15541 cgatgtctgc aatcggtact agtcaggaaa gtgcccgacc agaaacattg cggcaattag
    15601 ccattatgaa taaaagttgt ggtgttgatt tacaacatat tggagatgct ggatacgggg
    15661 gattggcacc agcagaaaat attttcgaaa cctcggttgc aattagagga atgcgacata
    15721 ccatcaactg cgttagccgc tcgatcaatc gctaacaaaa ttaaataaaa aatggttaaa
    15781 ttgattattt aagtgggaac atacttgaga tatcagctaa cttctgtcac tttaaaaaag
    15841 ggactgtgac agaagtgaaa atagacaaaa aatatccgaa caagagacta ggatagcaga
    15901 aaaactatcg ttttgcaacg aggaccaact ggaccgtagg agcaattctc ttattgttcg
    15961 gatatttcgc ttgttcaagg acttatgtct gtagtgccct tttttggtag gaaaaatggt
    16021 agggaaccat atcattttta atgaaaatca agcatttcaa aagttgaaa
//
LOCUS       DACCXW010000063        15481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCXW010000063 DACCXW010000000
VERSION     DACCXW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.063"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1689
                     /locus_tag="IVY71_001685"
     CDS             <1..1689
                     /locus_tag="IVY71_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015968361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985739.1"
                     /translation="TTDINDCRVWSWGVCDIDNVDNITFGLDIDSFFEWCEMQGSADI
                     YFHNEKFDGEFMLSWLFKNGFKWAKETKEERTFSTLISNMGQWYALEICWNVKCTTTK
                     TGKTKKEKQRTIIYDSLKKYPFPVKEIAEAFNFPIKKGEIDYTKKRPIGYNPTEDEWD
                     YLKNDIQIMAMALKIQFDQGLTRMTRGSDALGDYKDWLKATHGKSTFKQWFPILSLGF
                     DKDLRKAYKGGFTWVNKVFQGKEIGDGIVFDVNSLYPSQMYVRPLPYGTPLFYEGEYK
                     PNNDYPLYIQNIKVRFRLKEGYIPTIQVKQSSLFIQNEYLESSVNKLGVDELIDLTLT
                     NVDLELFFEHYDILEIHYTYGYMFKASCDMFKGWIDKWIEVKNTTEGARKANAKGMLN
                     SLYGKFGTNPDITGKVPYMGEDGIVRLTLGEEELRDPVYVPLASFVTAWGRYTTITTA
                     QRCFDNIIYCDTDSIHLTGTEVPEAIEHLVDSKKLGYWKHESTFQRAKFIRQKTYVEE
                     IDGELNVKCAGMPDRIKELVTFDNFEVGFSSYGKLLPKRTQGGVVLVDTMFTIK"
     gene            1702..1872
                     /locus_tag="IVY71_001686"
     CDS             1702..1872
                     /locus_tag="IVY71_001686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985740.1"
                     /translation="MDLYKAIFIVRDEGTIDGFDTEHYVDISLHDFEEIYAKETREIE
                     AVTLVKTGNFKK"
     gene            2139..2429
                     /locus_tag="IVY71_001687"
     CDS             2139..2429
                     /locus_tag="IVY71_001687"
                     /inference="COORDINATES: protein motif:HMM:NF022859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985741.1"
                     /translation="MEIKEHEAILNGILESVTDGEARSKIVEHLEALREDYGATTEAL
                     TTANSTLDKLKKDNEALVISNSKLFRERASVEPTENNEPETDQNITLDDLGI"
     gene            2443..3771
                     /locus_tag="IVY71_001688"
     CDS             2443..3771
                     /locus_tag="IVY71_001688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Other names: p8; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head protein"
                     /protein_id="HAP7985742.1"
                     /translation="MADKITEQDVLNATNVETPVQLMTAIYNSSSSLFQANVPMPNAD
                     NIQAVGAGITRLDVVKNEFISTLVDRIGKVVIQYKSWRNPLKMFKKGNMPLGRTIEEI
                     FIDIAQEHKFNPEESVTGVFKQEVPDVKTLFHEINREGYYKQTIQEAWLEKAFTSWDN
                     FNSFVAGVMNALYTGDEISEFEYTKLLISNYQEKELFKELEIGEITESNAKEFIRKIK
                     STSNKLEFMSSAYNAQGVKTSTPKSDQYVIIDADTDATVDVDVLAAAFNMSKTDFVGH
                     KIVIDEFPKKQGEESSNIVAVIVDSEWFMIYDKLYKTTSLYNPEGLYWNYWLHHHQLY
                     SSSQFGNAVAFVKTATKPVTKVAFASATDTVVKGATKDIALTFTPAEATNQQGEVVSS
                     APSLVKATVKETSGKATAVTVEGLEVGQSLVTFSAIGGQQATVLVTVTSD"
     gene            3783..5597
                     /locus_tag="IVY71_001689"
     CDS             3783..5597
                     /locus_tag="IVY71_001689"
                     /inference="COORDINATES: protein motif:HMM:NF028148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985743.1"
                     /translation="MARRYTHVKLLANVPFDNTYAHTRWCKTQQEQESYFNSFPVLNE
                     NTDCSYQRDTQLGGVFRVDKHKDALYACNYLIFKNEETYPSKWQYAFVTDIEYKNDNT
                     SFVTFEIDVLQTYRFDIGIRESFIAKEHPQLYYSNGIPFINTIEESLDYGREYTTTNV
                     TTFHPNDGVNFLVILTSEAMPVGDKEDKSGGSIVGGPSPYSYYLLPLNSYGEVYKPNG
                     AGNANFGEYMAFLTTKEPFLNKIVGMYITSYTGIPFIVDHANKTVRYNAGGSYKITLP
                     TYASDPTGTMKTFAFFCVKEARTFVPKRIDLVGNVYNYFREAFPFNVKESKLFMYPYC
                     LLEITDTKGHVMTIRPEYLTGGKLSVYVKGSLGISNKLMIEPIDYDVTNSSIITNLSD
                     KMLIDNDPNDVGVKSDYAAAFMQGNKHSLIAQEQNIRNTFRHGMGNSAMSTGGAIFSA
                     LASANPFVGLTNIMGAGQQVNNYVAEKENGLNLLAGKVADIENIPDNVTQLGSNLSFT
                     MGNFQNYYQLRFKQIKYEYATRLNRYFSMYGTKSNRVATPNLQTRKSWNFIKLKEPNI
                     VGTMSNDVLTRVKQIFSAGVTLWHTNDILNYNQDNGDV"
     gene            5612..6592
                     /locus_tag="IVY71_001690"
     CDS             5612..6592
                     /locus_tag="IVY71_001690"
                     /inference="COORDINATES: protein motif:HMM:NF017190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985744.1"
                     /translation="MSRRKGAGLARNNRYTAKSRPYPNEPYSSDVEEISYYEHYRRQL
                     TLLTFQLFEWENLPKSIDPRYLEIALHTNGYLGFFKDPTLGFMVCAGAEDGQIDHYHN
                     PIFFTANEAMYHKRYPVLRYDDDDDKSKCIMLYNNDLKVPTLPSLHRFALDMADINQI
                     SRVNRRAQKTPVIIQTDEKKYFSLLQAYNQIDENNQAVFVDKDMEFDESFNVWQTNAP
                     YVVDKLRSELNEVWNEVLTFLGINNANVDKTARVQTSEVLSNNEQIESSGNILLKSRK
                     EFCDRVNRVFGDELDGKIDVKFRTDAVRQLQLAAGQSKKDQMSGGLPSAT"
     gene            6582..7418
                     /locus_tag="IVY71_001691"
     CDS             6582..7418
                     /locus_tag="IVY71_001691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985745.1"
                     /translation="MLLKRYIESFTYYQPELSRKERIEVGRKQLFDFDYPFYDETKRA
                     EFETKFINHFYLREIGSETMGSFKFNLDEYLNLNMPYWNKMFLSNLEEFPIFDDMDYT
                     IDEKQKLLNEIDTNIKANRDESKNQTKQVDQTDNRNKNTRDTGTTDSFSRNTYTDTPQ
                     KDLRIASNGNGTGVINYATNITEDLSKETTSSTGVETNNDKTNQNTRSNASEKETKNT
                     DINKDQNQTKDTITRYKGKKGNTDYADLLEKYRRSVLRIEKMIFREMNKEGLFLLVYG
                     GR"
     gene            7424..7975
                     /locus_tag="IVY71_001692"
     CDS             7424..7975
                     /locus_tag="IVY71_001692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985746.1"
                     /translation="MVDFNHDKRFDGLPSVFKERFSKYPHTEYRYELLLDEEVSALIA
                     YLNEVGALVNDMSGYLNYFIEHFVEKLEEITTDTLKKWLSDGTLESLINDTVFANYIK
                     EIKRLQILVAETRANSVNILLTKNKPDVADDRTFWFKIQRDNTDYGDDPIDTMRIVAI
                     NKVSGWNTATGDIYLNIKGTEAV"
     gene            7975..8271
                     /locus_tag="IVY71_001693"
     CDS             7975..8271
                     /locus_tag="IVY71_001693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985747.1"
                     /translation="MADIRTQLTSEDGTDNLFPVSKAVNIMTNSGTNVEGELGALKQN
                     NETMNTSVQNAVVTANQAKDSVAELNVNVGKLTNRITTLESTVANLDGIRYVEV"
     gene            8393..8797
                     /locus_tag="IVY71_001694"
     CDS             8393..8797
                     /locus_tag="IVY71_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985748.1"
                     /translation="MADKNIQMKDKDQNRLMPVTIAKNVFYNETNLELTVVDIENKAN
                     EAKQTAQQAKTTADGLSTKIETATSTANQALAKGGTAQQTAEQAKTTANSASSVATTA
                     KNTADSAKQTATDLAVRVSSLENTTIQYSVLP"
     gene            8809..9231
                     /locus_tag="IVY71_001695"
     CDS             8809..9231
                     /locus_tag="IVY71_001695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985749.1"
                     /translation="MANKNIQMKDSNENNLYPSVRAENLLNLTSRAELTMTNCQLYTP
                     SDKTNAISYLGAVGMLEGMVKFTESLTNPVITTLPEGFRPIRIKRIGCLAKYYTPNPA
                     NTKEMVYVVITPDGRVSVNDNVGKIEYLSLDNCVFPLK"
     gene            9242..10576
                     /locus_tag="IVY71_001696"
     CDS             9242..10576
                     /locus_tag="IVY71_001696"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985750.1"
                     /translation="MEERIDIQMNKMKEENQKNYLLHPETNPTQVVFDESLHGNENQE
                     SFNEFVDKRKMTTTIDVSAYGVIADGVTDCTPILNKLLEEKSEMGITFYFPPCERDSY
                     YRFANTIELKRDVPIVTFLGSGETTLKFETMTAFNVNIESFNIDGFALWLPQGAQSGK
                     GIFFNDTRNYNRFDFDLFVRNCTLNEGTYVVVARGRGVTFENCLFSNISQAIIKTAFP
                     DVNSMWQGNDINSRGTGFRGFFVKHNRIHFCTAIIIDNEDDYQNVINFCEISSNTIEG
                     GVSYYRGYAHNLHVQNNNHFLAYGNRNALFEFQDVDQAYIDVDVYSRNSKVEGMNSTA
                     ISRLVVVYGHYRNLRITGKLYRCQGHVITLYGGGVNFYCDLMAQEAPLTDGYRFIQTA
                     DKRVNYDGFIVRGLSNSTKVNTPMIYKAPQTVFYNRKIDHVLTGPNASNVYN"
     gene            10984..11625
                     /locus_tag="IVY71_001697"
     CDS             10984..11625
                     /locus_tag="IVY71_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAP7985751.1"
                     /translation="MCHWERPGKLHIEERLDLAQAYSKHIDGSGGGGVKRCYGTPIKN
                     TNLDPSSFMSGQLFGTHAGNGRPNNFHDGLDFGSIDHPGNEMIACCDGTVTHVGTMGA
                     LRAYFVINDGTYNIVYQEFSYNQSNIKVKVGDRVKNGQVCAIRDADHLHLGFTKKDFM
                     TALGSSFVDDGTWEDPLKFLGQCFGDGDNDDNNKGKDDLIYLWLSDSLNGWKF"
     gene            11642..12034
                     /locus_tag="IVY71_001698"
     CDS             11642..12034
                     /locus_tag="IVY71_001698"
                     /inference="COORDINATES: protein motif:HMM:NF016961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985752.1"
                     /translation="MIEYITSWLANDNHMIYGLILWLMVAMTIDFVLGFTIAKFDKEI
                     DFSSFKAKAGIIVKVAEMILVVYFIPVAVKFGALGITMYVTMLVGLILSEIYSILGHI
                     STIDDNNNWTDYVKKFLDGTINRKDDIK"
     gene            12034..12909
                     /locus_tag="IVY71_001699"
     CDS             12034..12909
                     /locus_tag="IVY71_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25"
                     /protein_id="HAP7985753.1"
                     /translation="MNGIDISSHQTGIDLSKVPCDFVNIKATGGTGYVNPDCDRAFQQ
                     ALALGKKIGVYHFAHEKGLEGTPEQEGAFFLKNIQGYIGKAILILDFEGSNQKDSAWA
                     KAFLDYVYNQTGIKPWFYTYTANLNTTDFSNVAKGDYGLWIAEYGRNLPQGYSQPSPP
                     QTNNFPVVACFQYTSSGRLSGYNGNLDLNVFYGDRETWDLYVGKKSDQIVPPENKIFD
                     ATSDEFIFTLTKGSTSVFYFDGETIFELSDPTQLDHIRGTYNHVHGKEIPSLVWTPEQ
                     FDIYLKMYDKKPVYK"
     gene            12921..13952
                     /locus_tag="IVY71_001700"
     CDS             12921..13952
                     /locus_tag="IVY71_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015991957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA encapsidation protein"
                     /protein_id="HAP7985754.1"
                     /translation="MTNSLGVKLKEKNLYYSPNNALGFNCLMLFVIGARGIGKTYGYK
                     KFVVNRFIKHGEQFIYLRRFKTELKKIPQFFKTMAKEFPEHKLEVKGKEFYCDDKLMG
                     WAVPLSTWGIEKSNEYPDVRTILFDEFLIEKSKITYLPNEAEALLNMMETVFRDRTNT
                     RCILLSNATSVVNPYFLYFNLQPDLNKRFNLYQDRGILIELCDSKDFAEVKKQTPFGR
                     LIRGTDYEDFSINNEFVNDSDTFIEKRSKNSSFLCAIAFEGKIFGYWIDAETGCVYVS
                     YDYQPNTNHFYAMTTKDHEENRLLMKNWRNNYYLSTVAKAFKNSYLRFDNIVIKNLHY
                     DLFNKMKIW"
     gene            complement(13955..14128)
                     /locus_tag="IVY71_001701"
     CDS             complement(13955..14128)
                     /locus_tag="IVY71_001701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985755.1"
                     /translation="MRKDFVYINTPDPKANKKALAKITNAKEPKQNYRRLQSLCYLLF
                     IIVIELIVVTLVK"
     gene            complement(14125..14436)
                     /locus_tag="IVY71_001702"
     CDS             complement(14125..14436)
                     /locus_tag="IVY71_001702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985756.1"
                     /translation="MIARTFKEHRELIEWLRFYCKRNLSDNEKIEIIEGTLQDFDVPE
                     INITELFLTHSTLLPESSQFNILEKYCQAMNLVTSYVKVGSRYQLALQIPKGYLKEKE
                     L"
     gene            complement(14442..14732)
                     /locus_tag="IVY71_001703"
     CDS             complement(14442..14732)
                     /locus_tag="IVY71_001703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985757.1"
                     /translation="MINNLSLILEGLNQLTKEDNDSLASIKSEITQGGNQVILYIDYV
                     TKEFVLTHDKYNYVYLDSHCINIAITKSMKSVEHYAEQLKHDGYKQITDKWE"
     gene            complement(14805..15038)
                     /locus_tag="IVY71_001704"
     CDS             complement(14805..15038)
                     /locus_tag="IVY71_001704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985758.1"
                     /translation="MFNKALIAEKGEVSLLRPFNVWDMNYHIIDTENNKHYRIDIDDV
                     GDEEYCLLSFEELKELDMDLISEYSWKTTKITD"
     gene            complement(15111..15299)
                     /locus_tag="IVY71_001705"
     CDS             complement(15111..15299)
                     /locus_tag="IVY71_001705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985759.1"
                     /translation="MYFDILIELYDGDIEKTNLFTDTDKLKTYLYKILDKEDAEFLLE
                     NGKVVIDDYTLYLDSVTY"
BASE COUNT         5497 a         2387 c         2956 g         4641 t
ORIGIN      
        1 acaacagata ttaatgattg tcgcgtatgg tcgtggggcg tatgcgatat agacaatgtt
       61 gacaatataa cattcggttt agacattgat tctttttttg agtggtgcga aatgcaaggt
      121 agtgcagaca tctatttcca caatgaaaaa tttgacggag agtttatgct ttcatggtta
      181 ttcaaaaatg gtttcaaatg ggctaaagaa acgaaagaag aaagaacatt ctctacattg
      241 atatcaaata tgggtcaatg gtatgcttta gagatatgtt ggaatgtaaa atgcacaaca
      301 acgaaaacag gtaaaacaaa aaaagaaaag caacgaacca tcatttatga tagtcttaaa
      361 aaatatcctt ttcctgtaaa agaaattgca gaagctttta attttcctat aaaaaaaggc
      421 gaaatagatt atactaaaaa aagacctatt ggttacaatc caactgaaga tgaatgggat
      481 tatttaaaga acgatattca gatcatggcg atggcattaa aaattcaatt cgatcaagga
      541 ctaactcgaa tgactagagg aagcgacgct ttaggcgatt acaaagattg gctaaaagct
      601 acacatggaa aatcaacttt caaacaatgg tttcctattt tgtctttagg gtttgataaa
      661 gacttacgta aagcatacaa aggcggcttc acttgggtaa acaaagtttt tcaagggaaa
      721 gaaataggtg acggcattgt ctttgatgtc aactctttgt atccctctca aatgtacgta
      781 agacctttac catatggaac acctctattc tacgaaggag aatacaaacc gaacaacgac
      841 tatccgctgt acattcaaaa tatcaaagta agattccgtt taaaggaggg ttatattcca
      901 accattcaag tgaagcaaag ttcgttattc attcaaaacg aatatcttga atcaagtgta
      961 aacaagttag gagttgacga attaatcgat cttactctta caaatgttga cctagaatta
     1021 ttttttgaac actacgatat tttagagata cattacactt acggatatat gttcaaagct
     1081 tcttgtgata tgttcaaagg ctggatcgat aaatggatcg aagtaaagaa caccaccgaa
     1141 ggggctagaa aagctaacgc caaaggtatg ttaaatagct tgtatggaaa gtttggaaca
     1201 aaccctgaca tcacaggaaa agtgccttac atgggagagg atgggattgt tcgattgaca
     1261 ctaggagaag aagaattaag agatcctgtt tatgttccgc ttgctagttt tgtgacggct
     1321 tggggtaggt atactaccat tacaaccgct caaagatgtt ttgacaatat catttattgt
     1381 gatacagata gcattcatct aacaggaaca gaagttccag aagcaatcga acatcttgtt
     1441 gattctaaaa aacttggata ttggaaacac gaaagcacat ttcaacgagc aaaattcatt
     1501 cgacagaaaa catacgtaga agaaattgat ggcgaattaa atgtaaagtg tgcaggtatg
     1561 cctgatcgaa taaaagagct tgtaaccttt gacaattttg aagttggttt ttcaagttat
     1621 ggaaagttgc taccgaaaag gacacaaggc ggcgtggtat tagtagatac aatgtttaca
     1681 atcaaataag gaggaataat aatggattta tataaagcaa tttttattgt acgtgatgaa
     1741 ggaacgattg atgggtttga taccgaacac tatgttgata tctctttaca tgattttgaa
     1801 gaaatctacg caaaagaaac acgtgaaatt gaagcagtaa cattagtaaa aacaggaaat
     1861 ttcaaaaaat aaattattta catcctttgc aaagtatggt aaaatattct tgtgatagtt
     1921 aacaagagtc aaatttggcg agattgggcg aatgtacacg tgagagatcg tgcgctcccg
     1981 ttaagttatg gacacataaa cgttttgacc gttaaccaat cgcaaaaacc ttttaggagt
     2041 agcccttaaa tgtggctact cttttttgtg tttcacagat ttatgtttca cgtgaaacag
     2101 tttttatggt ataatagaat caaaaggagg tggagattat ggaaattaaa gaacatgaag
     2161 cgattctaaa tggtattctt gaaagtgtca cagacggtga agcaagatca aagattgtag
     2221 aacatcttga agcattgcga gaagattacg gagcaacaac agaagctttg acaacagcga
     2281 atagcacact tgataagtta aagaaagata acgaagcgtt ggtgatttca aactcaaaat
     2341 tgttccgaga acgagcgagc gtagaaccga cagaaaataa cgaaccagaa acagaccaga
     2401 atattacact agatgatcta ggaatttaag gaggaaacaa acatggcaga taaaattaca
     2461 gaacaagatg ttcttaacgc cacaaatgta gaaacaccgg tacaattgat gacggctatt
     2521 tataatagtt catcatctct ttttcaagcg aacgtaccta tgccaaatgc agataacatt
     2581 caagcggttg gtgctgggat cacacgttta gatgtagtaa aaaatgaatt tatttcaact
     2641 ttagttgatc gtattggtaa agtagttatt caatacaaat catggcgaaa ccctttgaaa
     2701 atgtttaaaa aaggaaacat gcctttaggt cgaacgatcg aagaaatttt tattgacatt
     2761 gcacaggaac ataaattcaa tcctgaagaa tctgttacag gtgtatttaa acaggaagtt
     2821 cccgatgtaa aaacattgtt tcacgaaatt aatcgtgaag gttactataa acaaacgatc
     2881 caagaagcat ggctagaaaa agcatttact tcatgggata atttcaatag ttttgttgcc
     2941 ggtgtaatga atgctttata cacaggcgat gaaataagtg aatttgaata cacgaaattg
     3001 ttaatttcaa actaccaaga aaaagagtta ttcaaagagc tcgaaattgg tgaaattact
     3061 gaatcaaatg caaaagaatt tattcgtaag atcaaatcaa catctaacaa attggaattc
     3121 atgagttctg cttacaacgc tcaaggcgtt aaaacatcta caccaaaatc tgaccaatac
     3181 gttattattg acgctgacac tgacgcaaca gttgacgttg atgttttagc agcagcattc
     3241 aatatgagta aaactgactt tgtaggacac aaaatcgtta ttgatgagtt tcctaaaaaa
     3301 caaggcgaag aatcgtcaaa tattgtggca gttattgtag atagtgaatg gtttatgatc
     3361 tatgacaaat tgtacaaaac aacaagtcta tacaatccag aaggcttata ttggaattac
     3421 tggttacacc accaccaatt atattctagt tcccaattcg ggaatgccgt tgcttttgtc
     3481 aaaacagcaa ctaaaccagt tacaaaagtt gctttcgcaa gtgcaacaga tactgttgtt
     3541 aaaggagcaa ctaaagatat tgcacttaca tttacaccag ctgaagctac aaatcaacaa
     3601 ggagaagttg tttcatcagc tccatcattg gttaaagcaa cagtcaaaga aacatcaggc
     3661 aaagcgacag ccgtaaccgt agaagggtta gaagtcggtc aatcattagt aacattctca
     3721 gctatcggag gtcaacaagc aacggttctt gttacggtta cttctgacta aggaggacaa
     3781 ttatggcaag acggtataca catgtaaaat tgttggctaa cgtgcctttt gataacacct
     3841 atgcacacac aagatggtgt aaaactcaac aggaacagga atcgtatttt aattcgtttc
     3901 ctgttctaaa cgagaatact gattgttctt atcaaagaga tacacaactc ggtggagttt
     3961 ttagagtaga taaacacaaa gacgctttat atgcttgtaa ctatctcatc tttaaaaacg
     4021 aagaaactta tcctagtaaa tggcagtatg cctttgttac agatattgaa tataagaacg
     4081 acaacacaag tttcgttact tttgaaattg atgttttaca aacttatcgt ttcgatattg
     4141 gcatacgaga aagtttcatt gcaaaagaac atccacagct ttattattcg aacggaatac
     4201 ctttcattaa tacgattgaa gagtcgcttg attatggtag agaatacaca acaacaaatg
     4261 taacaacttt tcatcctaac gatggagtca attttcttgt tattctaaca agtgaagcaa
     4321 tgccagtagg agataaagaa gataaatcag gaggatcaat tgtcggaggt ccatcgcctt
     4381 attcctatta tttacttcct ctcaattcat atggcgaggt atacaaacca aatggtgcag
     4441 gtaatgctaa ctttggagag tacatggcgt ttcttacaac gaaagaacct tttttaaata
     4501 agatagtcgg gatgtatata acgtcatata caggtatacc atttattgtg gatcatgcta
     4561 acaaaacagt aaggtataat gcaggaggtt cgtataagat tacactccca acctatgcca
     4621 gtgatccaac aggaacaatg aaaacattcg ctttcttttg tgtgaaagag gcaagaacat
     4681 ttgttcctaa aagaattgat ctggtaggga acgtgtataa ctactttaga gaagcttttc
     4741 catttaatgt taaggaatca aaactattta tgtatcccta ttgtttacta gaaattacag
     4801 atactaaagg acatgttatg actatcagac ccgaatatct tacaggcggt aaattgagtg
     4861 tatatgtaaa aggttcgtta ggcatttcta ataaactcat gattgaacca attgattatg
     4921 atgtaactaa ctcatccatt attaccaatt taagtgacaa gatgttaatc gataatgatc
     4981 ctaatgatgt aggagttaaa tctgactatg ctgctgcatt catgcaaggg aacaaacact
     5041 ccttgattgc tcaagaacaa aacatacgaa acacctttag gcatggtatg ggaaacagtg
     5101 caatgagtac aggaggggcg atcttttccg cattagccag cgccaatcca tttgttggtt
     5161 tgactaacat tatgggggct ggacaacaag taaacaacta tgttgctgaa aaagaaaatg
     5221 gattaaatct tttggcaggc aaagtagcag atatcgaaaa tatcccagat aatgtcacac
     5281 agctaggatc aaacttatct ttcacaatgg gaaactttca aaattattat caattgcgat
     5341 tcaaacaaat taaatatgag tatgctacaa gactgaatcg ttacttctca atgtatggca
     5401 caaagagcaa tcgagtagct acaccaaact tacaaacaag aaaatcatgg aatttcatta
     5461 aattaaaaga gccaaatatt gtaggtacaa tgagtaacga tgtgttaacg cgagtgaaac
     5521 aaatttttag tgcaggagtt accctttggc atacgaacga tattttaaat tataaccaag
     5581 acaacggaga tgtgtaggaa ggaggaataa gatgagtaga cgaaaaggtg caggacttgc
     5641 tagaaataac cgttatacag caaaaagcag accttatcca aacgaaccct attcaagtga
     5701 tgtagaagaa atcagctact atgaacatta tcgtagacaa ctcacgctcc ttacgtttca
     5761 gttgtttgaa tgggaaaatt taccgaaatc aattgaccct cgatatttag aaattgcatt
     5821 acacacaaat gggtatctcg gtttctttaa agaccctaca ctagggttca tggtttgtgc
     5881 cggtgcagaa gacggtcaaa ttgatcatta tcacaatcct atattcttta ccgcaaacga
     5941 agcaatgtat cacaagagat atcctgtttt aaggtatgat gatgatgatg ataaatcaaa
     6001 atgtatcatg ttgtataaca atgacttgaa agttcctaca ttaccaagtc ttcatcgttt
     6061 tgctttggat atggcagata taaaccagat atcacgagtg aaccgaagag cgcaaaaaac
     6121 acctgtaatt attcaaacag atgaaaagaa atatttttcg ttgctacaag cttataatca
     6181 aattgatgaa aacaatcagg ctgtttttgt ggataaagat atggagtttg acgaatcttt
     6241 taatgtatgg caaacaaatg ctccatatgt ggtagataaa ctacgatccg aattgaatga
     6301 agtatggaat gaagtgttga cttttttagg tattaacaat gcaaacgtag ataagactgc
     6361 acgtgtacaa acatcagaag tcttatctaa caacgaacag attgaaagct caggaaacat
     6421 cttgttaaaa tcaagaaaag agttttgtga tcgtgtaaat cgtgtctttg gcgatgaact
     6481 tgacggaaag attgacgtga agtttagaac ggacgcggtg cgacaattac aactagcggc
     6541 aggtcaatcg aaaaaagatc agatgagtgg agggttgcct agtgctactt aaacgttata
     6601 ttgaaagttt cacttattac caacctgaat tatctcgaaa agaacgtatt gaagttggca
     6661 gaaaacaatt gtttgatttt gattatccat tttatgacga aacaaaacga gcagaatttg
     6721 aaacaaaatt tatcaatcac ttttacttga gagagatagg ttcagaaacg atgggatcat
     6781 ttaagtttaa tcttgacgaa tatttaaatc taaatatgcc ctattggaat aagatgttcc
     6841 tatcaaatct tgaagagttt ccgatttttg atgatatgga ctataccatt gatgagaaac
     6901 agaaattgtt aaatgagatt gatacaaaca tcaaagcgaa tcgggatgag tcaaaaaatc
     6961 aaacgaaaca agtcgatcaa acagacaaca gaaacaaaaa tacacgtgac acaggaacaa
     7021 ccgattcttt ttcaagaaac acgtatacag acacccctca aaaagatttg agaattgcca
     7081 gcaatggaaa tggtacaggt gtaatcaatt atgcaacaaa tattacagaa gatttgagta
     7141 aagaaacaac aagctccacc ggcgttgaaa caaacaacga taaaacaaac caaaatacac
     7201 gaagcaatgc ttctgaaaaa gaaacaaaga atacagatat taataaagat caaaatcaaa
     7261 ccaaagatac gattacacga tataaaggta aaaaaggaaa cactgattat gctgacttac
     7321 tcgaaaaata tcgtagaagt gttttgagaa ttgagaaaat gatctttaga gaaatgaaca
     7381 aggaaggctt atttctcctt gtttatggag ggaggtagta acaatggtag attttaacca
     7441 cgacaagcgg tttgacggtt taccctcagt attcaaagaa cggtttagca aataccctca
     7501 tacggaatac agatacgaat tactattaga tgaagaagta tcggctttaa ttgcctatct
     7561 gaatgaggtt ggcgctttag ttaatgatat gagtggttat ttaaattact ttatcgaaca
     7621 ttttgttgag aagttagagg agatcacaac tgacacactc aaaaaatggt tatctgacgg
     7681 tacactagaa agtttaatca acgatactgt ttttgcaaat tatatcaaag aaattaaaag
     7741 attacaaatc ttggttgctg aaacacgtgc taacagtgtg aatattcttt tgacaaaaaa
     7801 taaacctgat gttgctgatg atcgaacatt ttggtttaaa atacagcgtg ataataccga
     7861 ttatggtgat gatcctattg acactatgag aatcgttgca atcaataaag ttagtggatg
     7921 gaataccgct acaggagata tttatcttaa cattaaagga acggaggctg tataatggca
     7981 gacattagaa cacaactaac aagtgaagat ggaacagaca atttatttcc agtttcaaaa
     8041 gccgttaata ttatgactaa tagcggcacg aatgtagaag gagaattagg tgcactcaaa
     8101 caaaataacg aaacaatgaa tacgtcagtt caaaatgctg tagttactgc caatcaagca
     8161 aaagattctg tagctgaatt aaatgtaaat gttggcaaac taaccaatcg aataacaaca
     8221 ttagagagta cagtggctaa tcttgatggt attcgttatg tagaggtgta atagggcagc
     8281 taaaaacaca ggtgattcac cgcaaaaaaa gtgcaactga tcttgctgtt cgagtaagca
     8341 gtttagagaa cacaacaata caatatactg ttttatcata ggaggaaaaa acatggcaga
     8401 taaaaatatt caaatgaagg ataaagatca aaatcgttta atgcctgtaa ccattgcaaa
     8461 aaacgttttt tataatgaaa ctaatttaga gttgactgtt gttgatattg agaacaaagc
     8521 taatgaagca aaacaaacag ctcaacaagc aaaaacaaca gccgatggat tgtcaacaaa
     8581 aattgaaaca gcaacttcaa ccgcaaatca agcgttggcg aagggtggta cagcacaaca
     8641 aaccgcagaa caagcgaaaa caacagcgaa cagtgccagt tcagttgcaa cgacagctaa
     8701 aaacacagct gattcagcaa aacaaactgc aactgatctt gctgttcgag taagcagttt
     8761 agagaacaca acaatacaat atagtgtatt accataggag gaaaaataat ggcaaataaa
     8821 aatattcaaa tgaaggatag caatgagaat aatttatatc caagtgttcg agcagaaaac
     8881 ttgttaaatt taactagtcg tgctgaattg acaatgacaa attgtcaatt atatacccct
     8941 agtgataaaa caaatgcaat ctcttatctc ggcgcagtag gtatgctcga aggcatggta
     9001 aagtttactg aaagtttaac aaatccagtg attacaacat taccagaagg ttttagacca
     9061 ataagaataa aacgtattgg ttgtttagca aaatactaca caccaaatcc agcaaataca
     9121 aaagaaatgg tttatgtagt aatcacacct gatggcagag taagtgtaaa tgataatgta
     9181 ggtaaaatcg aatatctatc attagataat tgtgttttcc ctctaaaata aggaggttcc
     9241 tatggaagaa cgaattgata ttcaaatgaa caaaatgaaa gaagaaaatc aaaagaatta
     9301 cctattgcac cctgaaacga acccgacaca ggttgttttt gatgaatcat tacatgggaa
     9361 tgaaaatcag gagagtttca acgaatttgt tgataaaaga aaaatgacaa ctacaatcga
     9421 tgtaagtgct tatggcgtta ttgcagatgg tgtaacagat tgtacaccga tcttaaataa
     9481 actactcgaa gaaaaaagcg aaatgggtat cactttttat tttcctcctt gtgaacgtga
     9541 ttcatattat cgctttgcta acacaattga attaaagcgt gatgttccaa tagtgacttt
     9601 cttaggatca ggagaaacaa cattaaagtt tgaaacaatg acggcattta acgtaaacat
     9661 cgaaagtttc aacattgatg gttttgcatt gtggttgcca caaggcgctc aaagcggtaa
     9721 aggaattttc tttaatgata ctcgaaatta caaccgtttt gactttgatt tgtttgttcg
     9781 caactgtact ttaaatgaag gaacatatgt tgttgttgct agaggtagag gggttacctt
     9841 tgaaaattgt ctattctcta atatctctca agcaatcatc aaaacagctt ttcccgatgt
     9901 aaacagtatg tggcaaggga acgatattaa tagtagaggt acaggattta gagggttttt
     9961 tgtgaaacac aatcgtattc acttttgtac agcgatcatt attgataacg aagatgatta
    10021 tcaaaatgtc attaatttct gtgaaatttc tagtaatacg atcgaaggtg gcgtaagtta
    10081 ttatcgagga tatgcacata acttgcatgt tcaaaacaac aaccattttc tagcatatgg
    10141 aaatagaaac gccttgtttg aatttcaaga tgtggatcaa gcttatattg atgtagatgt
    10201 ttattctcgt aactctaaag ttgaaggaat gaatagtaca gctatttcac gtttagttgt
    10261 tgtttacgga cattaccgaa acttaaggat tacaggaaag ttatatcgtt gtcaaggaca
    10321 tgttatcact ttatatggtg gtggcgttaa tttctattgt gatctaatgg cacaagaagc
    10381 acctttgaca gatggttacc gctttattca aacagcagat aagcgtgtta attatgatgg
    10441 gtttattgtt cgtggattgt ctaattcaac aaaagtaaat accccgatga tctataaagc
    10501 acctcagact gttttctata atcgtaaaat tgatcatgta ctaacaggac caaatgcaag
    10561 taacgtatat aactaggagg atatgagatg gcaactctta caaatgaaca aatagctaga
    10621 ggacaaacaa ttgctaaaat acttggtaaa catggctata ataaaaattc acaagtagga
    10681 gttgtcgcta atctccattg ggaatcggct ggattgaacc cgaacgccaa tgaatacggt
    10741 ggcggtggtt atggcttagg tcaatggacg cctaaaagca atctttatcg ccaagctcaa
    10801 atttgtggat tgtctaatgc tcaagctgaa acgttggagg gtcaagcaga gatcatcgct
    10861 caaggagata aaacaggtca atggatggat aatacaccta tttcttatcc aggatatact
    10921 aatcctcaga ccctctcggc atttaagcaa tccacaaata ttgatgttgc tacaatcaat
    10981 tttatgtgtc attgggaaag accaggcaag cttcatattg aagaaagact tgatcttgca
    11041 caagcttata gtaaacatat tgatggtagc ggtggaggtg gcgtaaaacg ttgctatgga
    11101 acaccgatca agaacacaaa tctcgatcct agtagtttca tgagtggaca gctttttggt
    11161 acacatgcag gaaatggcag accaaacaat ttccatgatg gtttggactt tggttcaatc
    11221 gatcatcctg gtaatgaaat gattgcttgt tgcgatggca cagtaacaca tgttggaaca
    11281 atgggagcat taagagcgta tttcgtgata aatgatggta cttacaatat cgtttatcaa
    11341 gagtttagtt ataatcagtc aaatataaaa gtaaaagttg gcgatagagt taagaatggt
    11401 caagtgtgtg ctatacgtga cgcagatcat ttacacttag ggttcacaaa aaaagacttt
    11461 atgacagcgt taggctcatc ttttgttgat gatggaacgt gggaagaccc tctaaaattt
    11521 ttaggtcaat gttttggcga tggagataat gacgacaaca ataaaggtaa agatgatctt
    11581 atttatttgt ggctatctga ttcattaaat ggttggaaat tttaataagg agaaaaatgg
    11641 tatgatagaa tatattacat catggttagc aaatgataac catatgattt acggtttgat
    11701 tttgtggcta atggttgcga tgactattga tttcgtgtta ggttttacaa ttgccaaatt
    11761 tgacaaggaa atcgacttta gtagttttaa agctaaagca ggcattattg ttaaggtggc
    11821 agaaatgatt ttagtggttt actttattcc tgtagcagta aaattcggtg cattaggtat
    11881 tacaatgtat gtaacaatgt tggttggttt aattttatca gaaatttata gtatactagg
    11941 acacatttca actatagacg ataataacaa ttggactgat tatgttaaga agtttttaga
    12001 cggaacaatc aacagaaagg acgatattaa atgatgaatg gtattgatat ttctagtcat
    12061 caaacaggaa ttgatctttc aaaagttcca tgtgactttg taaatattaa agcaacaggt
    12121 ggtacagggt atgtcaatcc tgattgtgat cgagcatttc aacaagcttt ggctttaggt
    12181 aaaaaaattg gtgtgtacca ctttgcacat gagaaaggct tagaaggtac accagaacaa
    12241 gaaggagcat ttttcttaaa gaatattcaa ggttacattg gtaaagcaat tctaattctt
    12301 gattttgaag gttcaaacca aaaagattca gcatgggcga aagcgtttct tgattatgtg
    12361 tacaatcaaa caggtattaa accgtggttt tatacgtata cagcaaacct caatacaaca
    12421 gacttttcaa atgtagctaa aggcgattat ggattgtgga ttgcggaata tggtaggaat
    12481 ttacctcaag gatatagcca acctagtcca ccgcaaacaa acaactttcc agttgtcgct
    12541 tgttttcaat atacgagtag cggtcgttta tcgggctata atggaaacct tgatttaaat
    12601 gtgttttatg gagatcgaga aacatgggat ttgtatgttg gtaaaaaatc tgatcaaatt
    12661 gttcctcctg aaaataaaat atttgacgcc acaagtgatg agtttatttt cacacttaca
    12721 aagggtagta caagcgtgtt ttattttgat ggagaaacga tctttgaatt gtctgatccg
    12781 acgcaactcg atcatattcg tggaacgtac aatcatgtac atggaaaaga aatcccatca
    12841 ttggtgtgga cacctgaaca gtttgatatt tacttaaaaa tgtatgacaa gaaaccagta
    12901 tataaatagg ggtgcatagt atgacaaata gcttaggcgt taaacttaaa gagaaaaact
    12961 tatactatag ccctaacaat gctttaggtt ttaattgcct aatgttgttt gtaataggcg
    13021 cacgtggtat aggtaaaacg tatggatata aaaaatttgt tgttaatcgg tttataaaac
    13081 atggcgaaca atttatttat ttaagaagat tcaaaacaga gcttaaaaag attcctcagt
    13141 ttttcaagac aatggcgaaa gaatttcccg aacataaact tgaagtcaaa ggaaaagagt
    13201 tttattgtga tgataaatta atgggttggg ctgttccgct tagtacatgg ggtattgaaa
    13261 aatctaatga atatccagat gttcgcacaa ttttgtttga tgagttttta attgaaaaat
    13321 caaaaattac ctacttgcca aatgaagcag aagccttgtt aaatatgatg gaaactgttt
    13381 ttcgtgatcg cacaaatact agatgtattc tgttgagtaa tgcaacgagt gtagtcaacc
    13441 cttatttctt gtatttcaat ctacagccag atttgaataa gcgttttaac ctatatcagg
    13501 atcgaggtat tttgattgaa ttgtgtgatt ccaaagactt tgcagaagta aagaaacaaa
    13561 cacctttcgg aaggttaata cgtggtacag attatgaaga ttttagtatc aacaatgaat
    13621 ttgttaatga tagtgataca tttattgaaa agagaagtaa gaatagcagt ttcttgtgtg
    13681 caattgcttt tgaagggaaa atatttggtt attggataga cgcggaaacg ggttgtgtat
    13741 atgtgagtta tgattatcaa ccaaatacaa accattttta tgcaatgact acaaaagacc
    13801 atgaggaaaa cagattgttg atgaaaaatt ggcggaataa ttattatctt tcaacagtgg
    13861 cgaaagcctt taagaatagt tatttaaggt ttgataacat tgttattaag aacttacatt
    13921 atgatttgtt taataagatg aaaatctggt aaccctattt tactagagtt accacgatta
    13981 gttctattac aatgatgaat agtagataac atagtgattg tagtctacga tagttttgtt
    14041 ttggttcttt ggcgttagtg atttttgcta acgccttttt atttgctttt ggatcaggtg
    14101 tgttaatgta gacgaaatct tttctcatag ttctttctcc tttaaatatc cttttggtat
    14161 ttgtaacgct aactgatagc gagaaccaac ttttacgtat gaagttacta ggttcattgc
    14221 ctgacaatac ttttcaagta tgttaaattg acttgattcg ggcaatagtg tcgaatgagt
    14281 taaaaaaagc tcggtgatat ttatttccgg aacgtcgaaa tcttgtaaag taccctctat
    14341 gatctctatt ttttcattgt ctgaaaggtt acgtttacag tagaaacgta accattcaat
    14401 aagttcgcga tgttctttga atgttcgtgc aatcatttta atcactccca tttatccgta
    14461 atttgtttat atccgtcatg tttcaattgt tccgcatagt gttcaacgct tttcattgat
    14521 ttcgttattg cgatattaat gcaatgacta tcaagataaa catagttata tttatcatgt
    14581 gttaacacga actcttttgt aacgtaatca atgtataaaa ttacttggtt tcctccttgt
    14641 gttatttctg acttgataga cgctaaacta tcgttgtcct ctttagttag ttgatttaaa
    14701 ccctctaaaa ttaatgataa attgttaatc atgtaaaaca ctccttttat attaatttga
    14761 tattgatacc accaatcaaa taagattggt agcattgtat taaattaatc agttattttt
    14821 gtagttttcc atgaatactc tgaaataaga tccatatcta attcctttaa ctcttcaaaa
    14881 gataacaaac aatattcctc atcgcctaca tcatcgatat ctatacgata atgcttattg
    14941 ttttcggtat ctatgatatg ataattcata tcccagacat taaaagggcg aagtagagat
    15001 acctctcctt tttcagctat taatgctttg ttaaacatat gaaacactcc ttttatatta
    15061 atttgatatt gataccacca atcggataag attggtagca ttgtatcgaa ttaatatgtt
    15121 acgctatcaa gatacaacgt gtaatcatca ataactacct ttccgttttc taataaaaat
    15181 tcggcgtcct ccttgtctaa aattttgtaa aggtaagttt ttaatttatc tgtatccgtg
    15241 aataggtttg ttttttctat gtcgccgtcg tataattcaa ttaaaatatc aaaatacatt
    15301 tttatcatcc tttctttatt acatctatat tatatcatgt atctacatat atgtcaatca
    15361 tttaattcat ttatttaatg atttatttga ttgtttttat gatcccttct ttattacata
    15421 tatagtatat catgcatgtt tgtatttgtc aacaattaaa ttcatataaa tgtagtttgg
    15481 g
//
LOCUS       DACCXW010000064        15380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCXW010000064 DACCXW010000000
VERSION     DACCXW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.064"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            119..2179
                     /locus_tag="IVY71_001706"
     CDS             119..2179
                     /locus_tag="IVY71_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985760.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2262..2831
                     /locus_tag="IVY71_001707"
     CDS             2262..2831
                     /locus_tag="IVY71_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7985761.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3006..3812)
                     /locus_tag="IVY71_001708"
     CDS             complement(3006..3812)
                     /locus_tag="IVY71_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985762.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3950..5464
                     /locus_tag="IVY71_001709"
     CDS             3950..5464
                     /locus_tag="IVY71_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7985763.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5867..6850)
                     /locus_tag="IVY71_001710"
     CDS             complement(5867..6850)
                     /locus_tag="IVY71_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7985764.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6855..7502)
                     /locus_tag="IVY71_001711"
     CDS             complement(6855..7502)
                     /locus_tag="IVY71_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7985765.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7587..8219)
                     /locus_tag="IVY71_001712"
     CDS             complement(7587..8219)
                     /locus_tag="IVY71_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7985766.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8235..8786)
                     /gene="coaC"
                     /locus_tag="IVY71_001713"
     CDS             complement(8235..8786)
                     /gene="coaC"
                     /locus_tag="IVY71_001713"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7985767.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8783..9520)
                     /locus_tag="IVY71_001714"
     CDS             complement(8783..9520)
                     /locus_tag="IVY71_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7985768.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9517..10506)
                     /locus_tag="IVY71_001715"
     CDS             complement(9517..10506)
                     /locus_tag="IVY71_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7985769.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10764..11306
                     /locus_tag="IVY71_001716"
     CDS             10764..11306
                     /locus_tag="IVY71_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7985770.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11323..12408
                     /locus_tag="IVY71_001717"
     CDS             11323..12408
                     /locus_tag="IVY71_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7985771.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12420..13226
                     /locus_tag="IVY71_001718"
     CDS             12420..13226
                     /locus_tag="IVY71_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7985772.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13227..14054
                     /locus_tag="IVY71_001719"
     CDS             13227..14054
                     /locus_tag="IVY71_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7985773.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14051..15124
                     /locus_tag="IVY71_001720"
     CDS             14051..15124
                     /locus_tag="IVY71_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7985774.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4942 a         2779 c         3136 g         4523 t
ORIGIN      
        1 aatcaaaaaa aatactctcc taatagtcca tcttcttttg attttattta tataatgaaa
       61 tcatcttatg aaaattacta cttcatgttt agacaagagt agggaggact aagaataaat
      121 gtgttcaaaa agacgttcaa aacaactaaa taaacacgct tgtcgtttag ataaggaacg
      181 gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc acgatgctga tcagtttatc
      241 tgtaggtgtc tatatgcatg tacccgttca ggcaacgaca caaaccatca aggtcgtacg
      301 tacctctgtt tctgagacca ctgattcccc tatcactatt actcatactt tcactgatgc
      361 aaatggaaaa cagctacttc ctgcattgac ttcctataat aaagtcagtt atcctccaac
      421 gataaaccac tactggttaa ttcctaacaa aacattatgg acatcggaaa ccggaagcag
      481 tgtgccgctt tctatctttt ccgatatgac tgatcctaca aaaggtgatc agttaaaaca
      541 gatcactgat attttgaatc atgctttgac agatataaag gacggtggaa gcgcacaagt
      601 ttcttatgta tatgaaaaag attattcaca gtttgaaaga aagaaaatcg atgttctggc
      661 agggacaaaa gtttcgatat ctgatctggt agaaaggctg aagaacgcag atggaacaca
      721 aggagatatg tccaaagtaa tttcaaacaa tgggaatatc gtggatacga ctaatacagg
      781 aacatctact atttccttgt cctattttga tccaattgct ttaaaaacaa tgactgcatc
      841 tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa ggaaaagatc ttcacttaag
      901 aacaggagag agttacgccc ctaaagatct tgttgaatat gtaacaaaaa gcgatggaac
      961 ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta gatactacaa ctccgggaga
     1021 atatcccgtt gtattaactt atatagactc gaaaacatca aatgtggcag aaacaacagc
     1081 tgtagtttat gtagaagcat ctgctgcttc atcgactgtc atcactcatt ttatagatag
     1141 tcaaacaaat aaagaacttc aagcagaaac acatatgagt agtgtaaatc gtattcctga
     1201 tttgcgccta cctgctgaat tttctgatcg agtgccaaat atagatttat cgacttatga
     1261 acaaaatggg aaagtcactt ctatttctga ttggatgcaa gcaaacggat tagacagaaa
     1321 aagtcactgg tcggagatcc ttgaagtact tcactcagaa ctgatgaata ataggaagca
     1381 atcaggaggg gaaattacct ttacttatgt ttatatacca gacaatcgtt cattagaagg
     1441 tcatgatctt attgttcatc caaacgaaaa aataaacaaa gaagagttga tccaatcagc
     1501 aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta tcggttacaa tcaatgaagt
     1561 accattggag gatggttatt tgaccaaaca aacaggtgat tttacgatta tgtatagtta
     1621 tcatgacccg tattcactcc aagaactcca tgctcagtca ctattacatg ttttaccatt
     1681 aaacaaacag atagatcaaa aagaacaaaa aagacaagaa gaaagcttgt cagaaaaaaa
     1741 acaacaaatg gctcatgcag tttctccaac agggaaagta aaacaaacaa gaagcctaga
     1801 gttagcaaga atatctagaa aaatctcaga atctaataaa acgataaggc aagaagctca
     1861 tttggcccca caagtttctg gattggaaaa tgaaatttta gagcctaaaa taaaacaaca
     1921 atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag aaggaatcag atgaccagaa
     1981 ggaaacgaag aaaaaacgct tgtcaactat tgattccacc gatgagcatc tgcagccgca
     2041 agtaccactt cctagtccgg gaacacccgc acctggtgga ggtattcctt cttctgcttt
     2101 aggagatttc atgcgaggta tggcaggaac atgggtatac gctaatcgaa atgaatttga
     2161 cgcagatgac tggttctgat ttatatctct atctgttata gtcaaataag tagctgagtc
     2221 atatgttatt tggcataatg gagacgagga gggatagaaa aatgttagat aaaacgttac
     2281 cttacgcaga aatctggatg acaagaccac ttcaacaaaa tgttccaatc acgcctttag
     2341 cggaaggttt tgaatttcga ttttatcaaa aagaagatga atctgcttgg gcggagattg
     2401 agacttccgt tggggaattt caaacgacag atgaagcact tgagtatttt caacaaaact
     2461 ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt tgtagagaca aaaaaaggag
     2521 aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag agagcaagtg tatccggttt
     2581 ttcattggtt agcagtgaaa tcagattacc aaggaaaagg gctcgcaaaa gcattgactg
     2641 ccagagtctt gcaagaattt cctaccctcc atacggacgg accaatctat cttcacacac
     2701 agacatggag ccatcaggcg attgctcttt ataaaaaaat gggttttact tttattgctg
     2761 aaaatctaga cggcacacct aatccagatt atgaaaaagt tatgcaggtt ttaagtaaat
     2821 tagataaata acgaaatggt gaaataagtc ctcagtattg ggataaaaaa cgaaatagtc
     2881 attcctcata tcttactttt ttgagttaat actatcggat aactaatggc atgccataca
     2941 ttcaaaaaag aaggggcagt gacaaaagta ttgtcactgc cccttctctt gttttttagc
     3001 atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa tttttggtaa
     3061 tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat aaaattaaca
     3121 aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg gatgattgtt
     3181 ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat atctggaaca
     3241 tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg gatgatcgta
     3301 tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa ctcccacata
     3361 acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg agtcaagttc
     3421 atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc cccagctaaa
     3481 aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatagccata aaaatcactt
     3541 aactctccta aaactgttga actaaaaata aaagataaca aaatgatttc tagcatattt
     3601 ggaatctgta tctttaatat cttttccaga aaataaggga tttcaaataa aattaatgtc
     3661 agtaataaaa gcattgtatt tccccagtgt tggttccaga cctgatccac tagcataata
     3721 acgacgatga tacgtaataa aatatagatt ccgtttgtga ttctacctgc agtttttgta
     3781 ctgttcgtaa aaaaggtttt caaatgattc ataaaataac tccttcatgt atatccttaa
     3841 ttatactaag aagcgtgtgt ttaccaaagc tatatgactt aaagaaaaac agagttgata
     3901 ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg gggatacaaa tgaaaaaatt
     3961 agttgctggt ttattaacag ccgttgcact tcttgctatg agcgcatgcg gcgcagatca
     4021 gaaaaataca gcgcagacaa gttctagtac gaaggaatct gccgaagtat catcaggcgc
     4081 ttctgaacaa gtatatacag atccaaaaga aatgaaagaa tcttatgatg tcgtgattat
     4141 tggagctggt ggtgcaggga tggcagcagc tatcgaagca aaagatgctg ggttgaatcc
     4201 agtaattttg gaaaagatgc ctgttgccgg aggaaacaca tctaaatcat caagcggtat
     4261 gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc aaagactcga atgatgcctt
     4321 ctatgaagaa acgctaaaag gtggtggagg aacgaatgat aaagaaatgc ttcgatactt
     4381 tgtcgatcac tctgctgatg cggtcgattg gctagatcaa aacggtatca aactggacaa
     4441 cttaacgatc actggaggga tgagtgaaaa gaggacacat cgtccgtctg atggctcggc
     4501 tattggtgga tacttagtgg aggggttatt gaataacgtc catgagcggg aaatccctat
     4561 ttttgtcaac gcagatgtga cagacattaa gaaaaatggg gaaacggtca atgaagtgac
     4621 tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga aaagcaatcg tagttaccac
     4681 aggcggtttt ggtgccagca aagaatttat tgaaaaatat cgtcccgatt tagctgatta
     4741 tattacgaca aatcaagaag gatcaactgg agatggtatc accatgattg aaaaattagg
     4801 tggacaaacc gttgatatgg ataaaataca aattcatcca actgtgcagc aggatgaggg
     4861 ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc ttggtcgatc aagaggggaa
     4921 aagatttgtc aatgaaatgg atacaaggga taaagtatct gcagcaatta cagcattgcc
     4981 tgaaaaatca gcttatttga tttttgacca aggcgtgcgt gatcgtgcca cagctattga
     5041 attttatgac caaaaaggat atgtcacaac tggaaagaca atggaagaat tagcagaaaa
     5101 aatcgattta ccaaaagaaa cactagcaaa aacagttgat ggatggaata cggatgttgc
     5161 ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg gaacatgact tatcgtctcc
     5221 gaattattat gcgatcaaaa tcgcaccagg aatccattac acgatgggcg gtgtgaaaat
     5281 caatacgaac actgaagtat tagataaaga aaaccagcca attagggggt tatatgctgc
     5341 tggtgaagtc acaggtggct tacatggaaa caaccggatc ggtggaaatt cagttgcgga
     5401 gatcattatt tttgggcgac aagcgggcat acaagcagct cgatttgctt cggaaaatga
     5461 atgaaataga acgtaaataa aaagaggttg tgaaatcagc gttttgagct aataccgagg
     5521 gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa aagctgccct gcatcaagta
     5581 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggttgga cttattaccg
     5641 cagatgcaag cttttgaaca ccgtttattc ggaaaaagag cgtgggacaa aagtaaagaa
     5701 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagaaa ttccttcgga
     5761 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
     5821 ggagttaaac gcttctgtcc cagcctcttt tttgatcagc atgattttat tcagttatct
     5881 tcaaaatgat ttttccgatg tttttgtttg cttccatata ttcatgcgct tgtttgactg
     5941 cgctgaattg gaatgtacgg tcaatgattg gccgaatctt gccattttct agaaaaggcc
     6001 cagctttttg cataaaagtt tgtgcgagct ctgctttata ctgatcactt ctaggtgtca
     6061 ataatgtacc aagcagttgg acacgtttcc caatcaatac accaaggtct atctcaggca
     6121 cgatggtccc acctagcata ccaatcaaga cccaacgtcc atcaacggct atagaagcta
     6181 aattcttttc ccagtaagaa gcgccgatga aatccaaaat aacatcgact ccccgacctt
     6241 cagtgatttt tcgtacctct tcttgaaagt cttgtttttt atagttgatc aattcatctg
     6301 caccaagttc tttacaaaag gcgagttttt cttctgatcc tgcagtaacg atgattttcg
     6361 cgtgagaaag ctgtctagct aattggatcg ctgctgttcc cacaccactt gctcctgcat
     6421 ggatcaagac agtttcgcct tcttgtaatt ttccaagcca gtaaagtgtt tgataagcag
     6481 tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc tagcgtatca gggagaagga
     6541 ttgcacgatc ggcaggcatc acagcatatt cggcataacc acctagattg actaatccgt
     6601 atacttttgt cccaggagta aaagtggaga tatcactttt attctcaact acctctccag
     6661 ctacttcgac tcccaaaata gggtagggag gtttcaaaga gactgattct cttttcataa
     6721 tatctgttcg attgacagct gctgtatgga ctttgatcag taattcgcct tgttttggtt
     6781 taggaacagg cacttcaatt tgttctaata catctacaga accaggatgc ttgattgaaa
     6841 tggcacgcat ggatctattc ctcctttgcc cattcttgga atgctgtatt caactctttg
     6901 ctaccttcag aaagtactag agtcgtacga gaaatatccg tttcttccac tagagcggca
     6961 agtacagcag caactgtaga gcgagtgatc gtttttgatg ccttctcatt aggcttagtt
     7021 aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga ctggttgaag aatgacaaaa
     7081 tctaagttag ttcgtttcag ccattcatct gcataatgtt tagtaatata ataatctgtc
     7141 atggactcag gccattttgc tggttcatct gcataaacag cactgaccat cagatagcga
     7201 gagacatgcg cattttcagc tgcgatcact gttttgattg cgccatccaa gtctacttgc
     7261 agaagattct ttccttgaga gccggcagca aaaatcaata catcgatgcc tttgaatgct
     7321 tcggtcattt tttccacgga ccaagttaaa tcaaaagaga catacgaaac attctctttt
     7381 tttactaatg tttgggaagc aacatctctc acccctgcaa aaatttcatg ggaacttgga
     7441 gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg cacctacaac aaaaattttc
     7501 atttggatca tcctttcgac gtcaaatctt tccattcaat agtaacacca aaagagaaaa
     7561 aaatcagaga tcggctacga tgactatcag ttagtaagag aagggtgctt gaagtatctt
     7621 aacaagacat tgcatactgc tgcagtcgca agacctgcga cgattccttc aggtatacca
     7681 cttgtaaaaa taaccgctag aatagctgaa taaacagcat ttaaatttgt tccgatgact
     7741 ttggcataat cttgctggaa taaaaagtag atcatattca tcactaaaat cgtattggtc
     7801 aatgaaccgg aaagtccagc taagaataaa gaaacggaat ctgctttttg tttcattagt
     7861 ttgcgcagac ctttataaac gaaataagga accaccccaa tcaagatgcg aggaataaac
     7921 gcgatcagca aggctttcca gcttccatgt tctgttccga tgactgggat aaacggagaa
     7981 aagacaaaag acaaaggtgt ttggataact gtgctacgga tcatgctgat cagtccaaat
     8041 gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga tcgaagcaat gattacagga
     8101 atatgcatgg ttgttgcatt gattggaccg atagggataa aacctaaagg cgtaacagct
     8161 aataaaatca aaatagctaa aaacatcgca gttaatgtaa agttttttgt atttttcata
     8221 agtactcctt ttttctaagc atttctttca ttaagagtca gcatgacctg ttcgatgatc
     8281 gtctgaatat cagctaaggc accttttcca aaatctccac aagctaaaag agattctctt
     8341 ggttcaattt cctgatagcc gacagatctc aatgtttcca gattacgctg agtgatcggg
     8401 ttttgataca tattcgtgtt catggcaggg gcaatcaatt ttggtgttcc cactggaagc
     8461 gccattgcga ctgttgaaag aagatcgtct gcaatgccgt ttgctagctt gccgataatg
     8521 tttgctgtag caggcgcgat gagaaacaag tcagcttgtt ttgctagttc gatatgattg
     8581 attacctgcg gatttgattc ttgcatgacg tctgtatgga ctgcgcgctt cgacagagat
     8641 tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag acataatcac atctacctgg
     8701 gcacctaatt tgacgaattg acttgtaaga tcagcgcttt tgtaagcaga aatacttcca
     8761 gtaacgccta gtaagatctt tttcattttg attcctccag tctaattttc tcagtaatca
     8821 atgcagcaat ctcagattta ctttgagctt cttccactgt tccatcttta gataagagat
     8881 agccgtggtg tgtctcgcca tggatttccg tcaaatcatt tgcgaaaata aaatcagttt
     8941 tgttatttat cagactattt tttgcgactg ccagaagaga ttcctttggt acatctacta
     9001 atagtttgaa accaatgatt tttgcttttg gctgccattc acgtagataa gaaatgattt
     9061 ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt agaagagatc tttgaaacag
     9121 tggtatctgt ctgttcattt tccgcaaacc aattgtccag ttcttcttgc gttgaagaag
     9181 tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa agcaggagta aaatcactga
     9241 ctgccatact gtggataatc gcatcgtagt gttctttttt cgttagttct tctaattgta
     9301 aaaataagtc cagtgcagat tcgatcgtat aaatcgttat attggagtgt cttttcggtt
     9361 tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc cggattggca gctaaataat
     9421 cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt aatagcacgt acttgatcga
     9481 ttttttcagt cgttcctcca gctgtgatta gaatcttcat ttatggtcac ctacatttac
     9541 tagtgttctt ccttggtgtg ttccagcaag tagtttagat gctgtttcag gcaattgatc
     9601 cagagtaatc tcttggacga ccagttcatc tgcaatctgc aaatccgcca tctgttgcca
     9661 taaagaaaga cgttgatcta ttggtacatt gacagaatca attccaatca actggatgtt
     9721 tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt cccccagcat tgccacataa
     9781 agctactgca ccgttataag agatcaatgg aagaagactg cttagctgct cgccacctac
     9841 tgtgtcgatg acatagtcga tttgctgttt tcctaaagct tttgttgtct ctggcagaaa
     9901 ttcatcaggt cgtacgactt gcaaagcacc tagacttttt agccatgtga cagactcttc
     9961 ttttctggaa aaggcaatga ttgactggaa tcctagttta tgtaacaggg ctatcgctgt
    10021 actgcctaca ccgccagaag caccagtaac gacaactttt ttatcggagg tcataccttg
    10081 acggattaaa gcgttcacac acaacatagc agtaaaccct gctgtaccaa ggatcatcgc
    10141 ttgacgcagc gtcatatttt ttggtagagg aactaaccag tctcctggta ctttttgata
    10201 ttggctataa ccgcctggat gggtgacgcc aagcccaaaa ccagtagcaa gtacaagatc
    10261 ttcttttgaa aaacgtggat tgttactttg gacgacagtt ccggataaat caatccctgg
    10321 agtcatcgga tatgttcgta tcacctgacc agactcgctc attgccaatg catctttata
    10381 attgacatca gaataggcaa tttttaccaa gacttcattt tcagccaact cagtaaatgg
    10441 cagatcactg attgctgtat ggaaaacagg ttcctttatt acctgaaaag atcgaaaagc
    10501 gttcattcgt gagacactcc ttttaaaatt ttcttttatc ataccataaa gcctactctt
    10561 ttattcttgt tcttccgtaa atttagacaa aaattaggtg aaaaataaag aatcaaacaa
    10621 ctagaattta ttccaaaaga taaagaaata ttcagcaaaa aaaattttac aaagggttgc
    10681 ctttttcaat aaatttcgta tactaatacg agtggtagta aacaggaagt ttactattag
    10741 aaaacttcga ttcggaggaa attatggaaa ttggtgagaa gttaagaaat ttgcgcatcc
    10801 aaaagaattt gacacaagaa gaactaggcg aacgcacaga tttgtcaaaa gggtatattt
    10861 cccagttgga acgagattta agttcaccat caatggaaac attcttttct attcttgaag
    10921 tcttaggtgt gactccagaa gaattttttc atcaagaagc agcgaatctg caagtggttt
    10981 attcaaaaga agatcatacg gtttatttcg atgaagaaaa cgggtatgaa ttggaatggc
    11041 tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt gctgactttt gaaggtgcag
    11101 gagaatataa gacgtttgaa ccttcattat ctgaaacgtt tatttttgta ttagaagggg
    11161 aattagagtt aagcttagga gagacggtct atgtggcaaa agcaggacag tcgatctatt
    11221 atcaagcgac aaaacaacat caattaagga atcatctcaa aaaacgaaca aaagtattga
    11281 ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa acgtggggaa accaatcatt
    11341 tcatttgatc aagtagtgaa acgctatgat gatgaaacag tattaaaaaa agtaagtttt
    11401 gagatcgaac aaggaaaatt ttatacactg cttggtcctt ccggctgtgg taaaacaacg
    11461 atcctacgta ttatagctgg attcaccgaa gctacagaag gggacatcta ttttgaaggg
    11521 aaacggttga atgatttgcc agctaataag cgacaagtaa atacagtctt ccaagattat
    11581 gcactttttc ctcatatgaa tgtttttgat aacgttgctt ttggattgaa gatcaagaaa
    11641 atgtccaaat cagatatcga aaagaaggta aaagatgctt tgcgtatggt ccagcttcct
    11701 ggatacgaaa aacgggagat tagtgaaatg tctggtggtc aaagacagcg tgtagcgatt
    11761 gcacgagcaa ttgtaaatga accgaaagta ttgctattag atgaaccgtt atcggcgctt
    11821 gatttgaaac tacgtacaga tatgcagtat gaactgcggg atctgcaaca acgattagga
    11881 atcacgttta tttttgttac tcatgaccaa gaagaagcgt tggcaatgag tgatgagatt
    11941 tttgtcatga ataaagggaa aatcgtccaa agtggtacac ccgtagatat ttatgatgag
    12001 ccaatcaatc atttcgttgc tgattttgtt ggggagagca atatcgtgga cggtgtaatg
    12061 atcgaagata atctagttga gtttgttggt aagcaatttg aatgtgtcga tggcgggatg
    12121 cgtctaaatg aaccagttga agttgtttta cgcccagagg atttgacgat cacgacacca
    12181 gataaaggaa aactagtcgt aacggtcgat actcagctgt ttcgtggtgt ccattatgaa
    12241 atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc attcaacgaa aaaagctaaa
    12301 gaaggaagca aagtaggatt ggcctttgag ccagaagata tccatgtcat gcgtttcaac
    12361 gagtctgaag aagaattcga tgcacgtctt gacagctacg aagaataggg gggacaagaa
    12421 tgaaaacaat gcgcaggatc tattccgtcc cttatatttt gtggctcggt ttattcgtga
    12481 tcgcgcctgt attaatgatc atttatcaat ctttttttga tatgaatggg caattcacgc
    12541 tgaataatta tctatcttat tttacgtcgg ggacatcgct ttctatgacg atcaattctg
    12601 tatggtatgc atttttgatc acgtttttca cgctgatcat cagttacccg actgcttatt
    12661 ttttaacaaa actgaagcac aagcaattat ggctgatgct gattattttg cctacatggg
    12721 tgaatctgct gcttaaagct tacgcattta ttgggatttt cagtgtgcac ggcagcgtga
    12781 atcatttctt tgattttatc gggatcgggc cgaaacagat attattcaca gacttcagtt
    12841 ttctgttcgt ggcaacgtat atcgagattc catttatgat tatgccaatt ttcaatgcat
    12901 tggaagagat gaatccttct ttgatcagtg ctagtagaga tttaggcgcg aatagctttg
    12961 agaccttccg acgagtgatt tttccgttat cattaaatgg tgtgaaaagt ggtgtccaag
    13021 cagtatttat tccttctctt tctttattca tgctgactcg tctgattggc ggaaatcggg
    13081 tgatcacttt aggaacggct atcgaacagc atttcttggt tacacaaaac tgggggatgg
    13141 gttctacaat cggtgtgatt ttaatcattg ctatgttcat cgtgatgctc ctgacaggcg
    13201 agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa atggtcgtat ctttatttag
    13261 ggatcgtctt tttattccta tatgcaccga tcttctatct gattttttat tccttcaatg
    13321 aaggaggaac gatgaacagc tttacaggat ttacttggga aaattatgaa gctgtttttg
    13381 aagatacacg tttgatcatt atcgtattga atacgttcat gctggctttc ttatctgcat
    13441 tgatcgccac gatcatcggg acatttgggg ctatgggaat ctattacacg aaaaaacgac
    13501 gtgcacggac agcgttgttg agcatgaata acatcttatt agtctctcct gatgttatta
    13561 tcggtgctag tttcttgatt ttctttacac tactaggatt cggattaggt tttcagagtg
    13621 tattgctttc ccatattgct ttcagcattc caatcgtcgt cttgatggtc ttgccaaaat
    13681 tgcaggaaat gaacgattca atggtcgatg cggctagaga ccttggtgct aataatttcc
    13741 aagtagtcaa aaatatcatt ttgccttatc tttctccagg aatcatagct ggatatttta
    13801 tggcatttac ctattctctt gatgattttg cggttacatt tttcgtcaca ggaaatggct
    13861 ttacgacgct ctcagtagag atctattctc gtgcaagaca aggaatcagt ttagaaatca
    13921 atgcgctaag tgcgttagtt ttcttgttct ccatgatatt ggtcattgga tactacttta
    13981 ttagtaaaga aaaccgtcca aaacggctga agaaaaacag aagagtacgc gagggggtgg
    14041 cacggctgcg atgaaaaaat tacaatctct gtttttaggt attctcatca ttattgcggt
    14101 gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc atgagtggag caaaggtact
    14161 taccatctat aactggggag attatatcga tcctagtttg atcacgaaat ttgaaaaaga
    14221 atatggatac aaagtgaatt atgaaacatt tgattctaat gaagcgatgt tcacaaaaat
    14281 ccagcaaggt gggacaaact acgacatcgc tataccaagt gaatatatga tccaaaaaat
    14341 gatcaaagaa aaactagtac ttccattaga tcattcgaag atcaaaggat taaataatat
    14401 tgatccacga tttttagact tggattttga tccagataat cagtattcga ttccatattt
    14461 ttggggaact ttagggatcg tatataacga taaattcttt gatgcaaatc aaattaagca
    14521 ttgggatgat ttgtggaagc cggaattaaa agacagtttg atgctgattg acggtgcacg
    14581 agaagttatg gggctttctt tgaatagttt aggctattct ttgaatagta agaatatggc
    14641 acagctgaca gaagcagcaa ataaattgaa tcatttaaca ccaaatgtca aagcaatcgt
    14701 ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc tccgtggcag tgacattttc
    14761 tggcgaagct gccgatatga tgtgggaaaa tgaacattta cattatgtga tcccaccaga
    14821 aggatccaat ctttggtttg ataatatcgt tatgccaaaa accgtgaaaa ataaagaagg
    14881 tgcctatgat ttcatcaact ttatgctaga accggaaaat gcggcacaaa atgcagagta
    14941 tattggttat tctacaccaa atgaagaagc aaaagcgtta ttgccaaaag acatttcgtc
    15001 ggacgaacaa ttctatccat ctgatgacac gatctcccat cttgaagtct acaaagatct
    15061 aggatcgaaa tacctaggca tctataatga cctattctta gaattcaaga tgtataggag
    15121 ataatggcaa aactcagtaa gaatggggac ttatcaacgt tctaaagagc taaaaatcgt
    15181 cctttttcta tctgagtttt ccaatcttct accaaaagcg gatgaaacgc cgcttttttt
    15241 gtgttatttt attttttcta ccacgttttt ctaccagaga aaagtggaca aaaccattgt
    15301 aaaatagggc ccgagacggg ctagtaaatc taagattgaa aaagttacag aaggataaag
    15361 taattggaaa ctatgcaaat
//
LOCUS       DACCXW010000065        15273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCXW010000065 DACCXW010000000
VERSION     DACCXW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.065"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            19..1137
                     /locus_tag="IVY71_001721"
     CDS             19..1137
                     /locus_tag="IVY71_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7985775.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1265..2641
                     /locus_tag="IVY71_001722"
     CDS             1265..2641
                     /locus_tag="IVY71_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7985776.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3042..4622
                     /locus_tag="IVY71_001723"
     CDS             3042..4622
                     /locus_tag="IVY71_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7985777.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4820..5044
                     /locus_tag="IVY71_001724"
     CDS             4820..5044
                     /locus_tag="IVY71_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7985778.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(5249..5569)
                     /locus_tag="IVY71_001725"
     CDS             complement(5249..5569)
                     /locus_tag="IVY71_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7985779.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5717..7951)
                     /locus_tag="IVY71_001726"
     CDS             complement(5717..7951)
                     /locus_tag="IVY71_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7985780.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8430..8696
                     /locus_tag="IVY71_001727"
     CDS             8430..8696
                     /locus_tag="IVY71_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7985781.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8696..10423
                     /gene="ptsP"
                     /locus_tag="IVY71_001728"
     CDS             8696..10423
                     /gene="ptsP"
                     /locus_tag="IVY71_001728"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7985782.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            10554..11633
                     /locus_tag="IVY71_001729"
     CDS             10554..11633
                     /locus_tag="IVY71_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7985783.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            11650..12873
                     /locus_tag="IVY71_001730"
     CDS             11650..12873
                     /locus_tag="IVY71_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7985784.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            12962..13849
                     /locus_tag="IVY71_001731"
     CDS             12962..13849
                     /locus_tag="IVY71_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7985785.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            13935..14165
                     /locus_tag="IVY71_001732"
     CDS             13935..14165
                     /locus_tag="IVY71_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7985786.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(14222..15187)
                     /locus_tag="IVY71_001733"
     CDS             complement(14222..15187)
                     /locus_tag="IVY71_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7985787.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         4952 a         2627 c         3119 g         4575 t
ORIGIN      
        1 aagaaaggag gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg
       61 cttgaattat tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc
      121 gaaccgatcg ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt
      181 tactatttat tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg
      241 gctgtcgcaa ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc
      301 atcccaagtc tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa
      361 caggtagaaa gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc
      421 aataaatatt ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt
      481 ggagtatcca atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc
      541 attacagcac cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac
      601 atccaacagt ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg
      661 aataaaacat tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc
      721 ttcacatttt taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt
      781 gcgggattga caaacctaat tccatattta ggaccttatc taggactagc acctgcattt
      841 ctagtaacag tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc
      901 gtccaacagt tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc
      961 catccattaa cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt
     1021 atcttcttag gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa
     1081 atcgtcaaag aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta
     1141 gttttagtac acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg
     1201 ctatagtgcc acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc
     1261 gattatgaat gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt
     1321 tttgacattg atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa
     1381 gaatcgaatc gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat
     1441 attgcagaat ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat
     1501 tttgtctggt gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg
     1561 cggcacagcg gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc
     1621 gattgttttt ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc
     1681 aaagttcact tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct
     1741 gctttctgct tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga
     1801 ttcaaaaatg accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa
     1861 tgaagaatta gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt
     1921 catggtccca cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt
     1981 gaatgaagtt ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa
     2041 agtagtcgga atccttcata caaagaattt actaaaagct gcccataaat tcggctttga
     2101 taatttagat atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat
     2161 tgatgatctg ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga
     2221 atatggcggc gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga
     2281 aatcgatgat gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata
     2341 tatcatccaa ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca
     2401 tatgagtgat gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga
     2461 tgaaggcgaa aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga
     2521 aggttctcga atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga
     2581 acctgaagaa gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata
     2641 attggaaact tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt
     2701 ttagtcagct gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga
     2761 atccatgcta tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc
     2821 attaagtaat aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct
     2881 tattgctgat aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc
     2941 ttttgtcaca gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag
     3001 agaaaatata aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa
     3061 agcaacaagt ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa
     3121 caacgattac agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa
     3181 aaggcaaaaa aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg
     3241 ggatttctgt tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct
     3301 tggatacacc aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg
     3361 atagtgcggt catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat
     3421 tccaagttgt aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg
     3481 atggtcgtga accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt
     3541 atccgatgaa ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc
     3601 ataatcgtgt agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg
     3661 atgaagatgg actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag
     3721 tcttagaaga agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg
     3781 ctcgtggcga tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga
     3841 cttttttaga gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg
     3901 atcagatggt aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa
     3961 atatgaatcc agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg
     4021 aacgaggaat ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca
     4081 cacaatttat ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg
     4141 gtgtatatga tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag
     4201 ttgcttatga agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa
     4261 atgtcatgaa acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga
     4321 ttcagcttta taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac
     4381 aattcgaagt attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc
     4441 caatgggaca caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt
     4501 caagtcgcaa tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc
     4561 aatttgcaga acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt
     4621 aaagaagtct tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag
     4681 cctctcatat tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt
     4741 aaaatcatag aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa
     4801 agaaaggaga gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa
     4861 aaaagaagag atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga
     4921 gcaaattgca caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga
     4981 cacgcgtaga actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag
     5041 ctagaaagaa aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca
     5101 caaaaatttg aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc
     5161 tttcgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
     5221 ctctttaatt attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt
     5281 ttggaatcga gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt
     5341 aaacggcgtc tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct
     5401 ggattttacg tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga
     5461 caatagtggt atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct
     5521 ggtttaatac aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct
     5581 cttttatgta tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac
     5641 agtattttga cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca
     5701 cgctataaaa ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg
     5761 gtttagaagt aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag
     5821 aagggtgatc aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc
     5881 gaagaggacg agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat
     5941 tcgtaggaac ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca
     6001 ttagtgtgac gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt
     6061 caaaacggtt cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag
     6121 taacaccttc gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat
     6181 tacttgtcat aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca
     6241 atcgaccatc atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta
     6301 cttcatctaa caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag
     6361 cttcttcgta tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt
     6421 ccatatattc agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg
     6481 caagttgttt cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc
     6541 cgattggacg attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc
     6601 gatctacagc gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca
     6661 gttgtgtctg ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga
     6721 ttttttccat atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt
     6781 tcattttttg aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt
     6841 ctcgagaagc taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag
     6901 gatcaacgat ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg
     6961 ctttatccgg caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga
     7021 tggcctcatc agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca
     7081 aaatactgat cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt
     7141 ctaaagcagc gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt
     7201 gcaattctcc tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac
     7261 cagcagaacc agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg
     7321 cttgacggat ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag
     7381 taccttggac taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg
     7441 gtacatcacc atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac
     7501 ctggttctcc aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc
     7561 gtttgatttc gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg
     7621 tgatattgat tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac
     7681 cattgaagtt gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct
     7741 gttgcatttg gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat
     7801 cattttgggc catattagtc agacctccat tttgttgatt tttgattttt tgataacagc
     7861 tctgacagta atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg
     7921 cctcattctt gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg
     7981 gtacatccat attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta
     8041 agagatttat gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt
     8101 attatactaa aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc
     8161 aaaggaaaga atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac
     8221 gtttgttata aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt
     8281 atctgaaatt gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg
     8341 aaagggtggc atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa
     8401 caacactcac ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag
     8461 aaactgggat ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact
     8521 ctgatgtaaa cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta
     8581 tgtctttagg tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag
     8641 ctgatgcaat ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt
     8701 tgaaatgcta aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct
     8761 agttcaaccg gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc
     8821 tcgtttggat gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc
     8881 agcgcaaagc ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc
     8941 agatccagaa atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga
     9001 atcagcgcta aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa
     9061 tgcttatatg caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca
     9121 cttattaggc gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc
     9181 acatgacttg actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt
     9241 aacagacatt ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc
     9301 agcaatcgtt gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt
     9361 caacgggatc gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga
     9421 aaaagcaggt gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga
     9481 aactgtcaca gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga
     9541 tctagttggt gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct
     9601 gtatatggat tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt
     9661 attagaaggt atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa
     9721 agaactacca tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt
     9781 gcgtatcagt ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct
     9841 tcgtgcatct gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga
     9901 attccgcgca gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa
     9961 ggaagtatct gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt
    10021 agcagacaag tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca
    10081 atacacaatg gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa
    10141 cccatcaatt ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg
    10201 ggcaggtatg tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat
    10261 gggtctagat gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa
    10321 acgcttggac acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat
    10381 gatggaagaa gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct
    10441 ggtgtggctt gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg
    10501 tcagtaagaa tagacaaaaa tagggcttaa gtttgagtga cagtttgtag gctatgttat
    10561 actatataag tgaaggggga cgtaaggtga aggtattatt atattttgaa agtgaaaaga
    10621 tgcttgcaaa atctggaatc gggcgtgcgc ttgatcatca aaaacgtgct ctgacggaag
    10681 tcggtatctc ttatacacta gatgaaaaag aagattatga catcctgcac atcaatacat
    10741 acggaatcaa tagtcataat atggtcaata aagccagaag agaaggaaaa aaagttgtct
    10801 atcatgcgca ttctacagag gaagatttcc gaaattcctt tatcagatcg aaccagcttt
    10861 ctcctatcgt aaaaaaatat ctggtaggat tgtaccaaaa agcggactat ctgatcacgc
    10921 caacacctta ttcaaaacag ttgttggaaa gctatggtat ccgtgtgccg attcaagcta
    10981 tttccaatgg gatcgactta gagaaatatc accctgatcc aatgaaagaa caaaagttca
    11041 gagaatattt caaactgtct ccggatcaaa aagtaatcat ttgtgtcggt ttgtttttcg
    11101 aacgtaaagg aatcattgat ttcgtagaaa ttgctaagaa gatgcccgaa tacacattta
    11161 tttggtttgg tcatgttccc atgtactcga ttcctcggaa catccggaaa attgtaaaag
    11221 aagatcatcc tgaaaatgtc ctgtttcctg gttacattcg tggagagatc atagaaggtg
    11281 cgtattcagg tgcagatcta ttctttttcc cttcttatga agagacagaa gggatcgttg
    11341 ttttagaagc tttagcaagc aaacaaaacg tacttgttcg agatattcca gtgtataatg
    11401 gctggttaga agatagcaag aactgctata tggggaagac aaacgaagaa tttatccgac
    11461 tgatccaaca gattaccaat caagaactac cgagtacgac acaagccggt tatcaaacag
    11521 caaaagagcg aagtatcaat aaaatcggtg aagaacttaa aaatgtatac gaaagtgtac
    11581 tgaatgaaaa agtctctagc aaattaaaaa aagtaaatca ggtcaaggac tagtttggag
    11641 gcgaaaaaca tgaaaatcgg cttttttacc gatacctact ttccgcaagt cagcggagtt
    11701 gctacatcga tcaaaacatt aaaacaagaa ttagaaagac atgggcatga agtctatatt
    11761 tttacaacga ccgatcctaa tgcggataag ttggaaaaag atgtgattcg aatgccaagt
    11821 gtaccattta tttctttcaa ggatcgaaga gttgttgtta gaggcatgtg gtatgcttat
    11881 ttggttgcca aagaactaga actagatctt attcataccc atacagaatt cggagcaggg
    11941 atacttggga agatggtagc taagaagttg aaaatacctc tgatccatac ttaccatacg
    12001 atgtatgaag actaccttca ttatatcggt aaaggaaaag tgatcaggca atcccatgta
    12061 aaatacttgt ctagattatt tgcaaatcat acgacaggtg tagtttgtcc aagtgaacgt
    12121 gttattgata cattacgcag ctatggtgta atagcaccgc ttcgcgtgat tcctactggt
    12181 atcgacattg aaaaattcaa acgttctgat atcactcagc aggatatcag caaattaagg
    12241 gagtcactag gtcttcagga aaaccatttg atgattcttt ctttaagcag gatttcttat
    12301 gagaaaaata tccaagcctt gattaatggg tttcctcagg tcatctccgc ttatcctaac
    12361 gccagattag ttatcgtagg aaaaggacct tatgtagaag agttagagga attgatctca
    12421 gaactgcagt tagaagaatt tgtgcagttc acaggagaag tggacaacaa tgatgtagca
    12481 ctttattata aagcggcaga ttattttgtc agtgcttcta cttcagaaac acaagggctt
    12541 acttatacag aagcgatggc agccggaaca ccttgtgtcg tggaaggaaa tgcttatttg
    12601 aaccgattgt tcgatcatcc ttcattaggg aaaacttttg aaaccgacga agactttgca
    12661 ccagcactta ttgattatat ttccagtgaa gttcctaaag atccaaaagt tttagaagct
    12721 aaactttatg atatttcatc tacacatttc ggcgaagcta tgatcgaatt ttatcaagac
    12781 atggttgttt atcatgaaga agagatgcga agaaaagaag aagaagactc gattgaacga
    12841 atcaaaataa aattaaattc atttaaaaga taaaattcaa acatttttta tgaaaaaatc
    12901 cttgtttcta gcgtatacta gcatagaagc aaggattttt gatttttagg aggtcgaaaa
    12961 aatgaaactt ggttttatcg gaacaggcgt catgggaagt gctgtcgcac gccatctact
    13021 agaagcagga cacgaagtag ctgtctataa tcggacaaaa gcaaaagctg atcctttagt
    13081 aacagaagga gctatctggg cagatacacc aaaagcggta gcagaacaaa gtaatattct
    13141 ctttacaatg gtaggttacc ccaaagatgt agaagaaatc tactatggac agtcggggat
    13201 tttttctgca gatatctcag gacatatttt agttgatttg actacaagta caccaagcct
    13261 ggcagaaaaa attgcaaaaa cagcaaaaga aaaaggcgca gatgcgttag atgcaccggt
    13321 atccggtggt gacttaggag caaagaatgg tacactgacg atcatggtcg gtggagaaga
    13381 agccgtttat gatcaagtac tgcctttatt caaagaattt ggtacgacat ttacattgca
    13441 tggttctgct gggaaaggtc agcatacaaa aatggctaat cagatcatga ttgcaggtac
    13501 gatgacagga atgacggaaa tgcttgtcta tgcacaaaaa aacggacttg atttaaagaa
    13561 agtgattgag acgctttcag gtggaagtgc agctaactgg tctttaagca attattctcc
    13621 tcgtatttta aaagaagatt acacacctgg tttttttgtc aaacatttta tcaaagattt
    13681 aaagattgca ttagaagaag cagaaaaaat ggatcttgta ttgccagcaa ctacccaagc
    13741 attgaaacta tatgaagagt tggcagataa aggttttgag aatgacggaa cccaagcttt
    13801 gatcaaactt tggtggcctg aaggaaaaat accagaaaaa aaatcataaa aaacgagttt
    13861 ttttagagaa aatcaatgca agtaggctat gcttttgata caatataaca gaaataagaa
    13921 aaggaggacc tccgatgaca cattcacaat tagtcgcaat cataaaacga ctggaggcta
    13981 tggtcgagtc agcagataat gaactgcaag ttcgccgttt tgaaaaagaa ggcgtagaga
    14041 agtgtacagt aacttatgat aaagcgaccg atacatttga attaactgaa acagatactc
    14101 atcagcaata cgaatttgat aatatcgata tcgtagcgat ggaaatctat gacttaatcc
    14161 aataaatttt aggataattg acatttgaag ttgaagatgc ctaaaagaag ccgagaagca
    14221 actaatttag ttgcttttca gcttcttttt tgtttaacag ttcaatttca aactgttctt
    14281 tttctaattt tttgcggtaa ctgatcaaga tttgtacagg atcgatttct tcttttgaca
    14341 attgacgttt caaagataaa tgagcttctt tcttttcata atagaagtca ggagaaacgt
    14401 caccagaggt aatcattgtt tctgcaagtt gaccataaca aacgaggtag tgctttttct
    14461 gactctcatc tgctgctaat ggcatgctga aggtgagcga tggtgttctt aaaaactgct
    14521 tttgttcctg gatccgtcta ataaaataag taactaattc gatatctttt tgaagcatac
    14581 cggctttttc gaaattcaac tgatagatga aatgttgttg tctttttttc aagtaagtca
    14641 gtagcttttt atttcggcct tgaaacagcc cttggagttc cttttttttc tgttcaaaag
    14701 tcatttcctg taggtcagga agctggactc gcaaagctaa caaactaatt tcagacaacc
    14761 aaggcatttg ataagtttct tccattatac gcaaaataga cggcattttt ttatagagac
    14821 gaaaaggccc gtatgttcga gccgtaggag tatctgttaa tacaaatccc ttattggtaa
    14881 tatgtacata attataatta ctgaaataat tcatttgtcg gttataaaaa ggacggtaat
    14941 gttggatcaa ttgacactcg agcaataaag catctaattc cgtgtctact acgacgaaat
    15001 caaaatctgt tatatgatga atcatacgta atactttttt tgaatgctgt ttattctgat
    15061 gaaaatagct gcttactcta tttttgagcc gttttgcttt accaacatag atgaccttac
    15121 catctcggtc tttcataaga tagacacccg gcagcatggg gagagttttc attttttctt
    15181 ttaacatagg atctcctttt acgctttgaa taaaaagact ataacaaatt ttgaaataat
    15241 ggacaagttt tagggaattc tatagttctg ttc
//
LOCUS       DACCXW010000066        14850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCXW010000066 DACCXW010000000
VERSION     DACCXW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.066"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            222..1961
                     /gene="yfcC"
                     /locus_tag="IVY71_001734"
     CDS             222..1961
                     /gene="yfcC"
                     /locus_tag="IVY71_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7985788.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2118..3302
                     /gene="iadA"
                     /locus_tag="IVY71_001735"
     CDS             2118..3302
                     /gene="iadA"
                     /locus_tag="IVY71_001735"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7985789.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3413..3616
                     /locus_tag="IVY71_001736"
     CDS             3413..3616
                     /locus_tag="IVY71_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7985790.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3717..4379)
                     /locus_tag="IVY71_001737"
     CDS             complement(3717..4379)
                     /locus_tag="IVY71_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7985791.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4392..5894)
                     /locus_tag="IVY71_001738"
     CDS             complement(4392..5894)
                     /locus_tag="IVY71_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7985792.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6442..8121
                     /locus_tag="IVY71_001739"
     CDS             6442..8121
                     /locus_tag="IVY71_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7985793.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8248..8727
                     /locus_tag="IVY71_001740"
     CDS             8248..8727
                     /locus_tag="IVY71_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7985794.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8946..9626
                     /locus_tag="IVY71_001741"
     CDS             8946..9626
                     /locus_tag="IVY71_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7985795.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9634..10965
                     /locus_tag="IVY71_001742"
     CDS             9634..10965
                     /locus_tag="IVY71_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7985796.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10980..11942
                     /locus_tag="IVY71_001743"
     CDS             10980..11942
                     /locus_tag="IVY71_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7985797.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12053..12391
                     /locus_tag="IVY71_001744"
     CDS             12053..12391
                     /locus_tag="IVY71_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7985798.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12470..13177
                     /locus_tag="IVY71_001745"
     CDS             12470..13177
                     /locus_tag="IVY71_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7985799.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13280..14761)
                     /locus_tag="IVY71_001746"
     CDS             complement(13280..14761)
                     /locus_tag="IVY71_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7985800.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4752 a         2545 c         3178 g         4375 t
ORIGIN      
        1 aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat ataaatggaa
       61 agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag tgattttcta
      121 ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt atccattttt
      181 tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa acaaattctt
      241 caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat gtcattattt
      301 ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga aagttcagca
      361 cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact gttttacgtc
      421 aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat caattagaag
      481 aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa
      541 aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa atctccttga
      601 cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct aaaaaattgc
      661 ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc ttaaattttg
      721 tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc gctgcattga
      781 tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc gacgcaggta
      841 tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg ctattattct
      901 ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt ccattctcaa
      961 tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca gtaaccgtga
     1021 cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt agcgtagcgg
     1081 tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga ttgattatgt
     1141 gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa aaaatccgta
     1201 aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct catatcaaaa
     1261 agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta ttagagatgt
     1321 tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac attccagaaa
     1381 tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt cttttcaaat
     1441 tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca gctgatttag
     1501 ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg ggaggatctg
     1561 atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg
     1621 gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg
     1681 ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct ccactggcag
     1741 atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt tcaggtttta
     1801 tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt gcacggatcg
     1861 aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt gttttaggaa
     1921 ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga cctgtaaata
     1981 aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg gtaaaatcaa
     2041 aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag ttatacttaa
     2101 agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca ccagattact
     2161 taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa gaacagctag
     2221 aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa gtactaaccc
     2281 cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc ggttatcaaa
     2341 atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg acagttgtcg
     2401 gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc aaagcgaaag
     2461 gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga ttgccggtaa
     2521 aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt atcggaatcg
     2581 gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag ttcgctcgtg
     2641 cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc atcaatgtcc
     2701 acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa gaaacagaaa
     2761 ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta tttgaagctt
     2821 gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa gatcctgatt
     2881 tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga ttgatcgagg
     2941 aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc ttgccttatt
     3001 ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca ttgctagtag
     3061 ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta cgagctataa
     3121 ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg
     3181 atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt atcgctaaag
     3241 gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa aaaagttttt
     3301 aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg
     3361 ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca caatggcaga
     3421 tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa aagaagcata
     3481 cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat cactaggtgc
     3541 tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa
     3601 agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa aaaatccgaa
     3661 ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc tgttttttaa
     3721 acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca gctttttgct
     3781 acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc gatttcttgg
     3841 aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg ctcatccgct
     3901 acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg ctgctgacct
     3961 cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac acgagccagt
     4021 cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc cattgctgct
     4081 atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa aataatcgag
     4141 acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc ttgatagtat
     4201 acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt cgtttttcca
     4261 gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag ttctacatct
     4321 ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt ccgtaacatc
     4381 aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat ccctatagaa
     4441 gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag tcccaattgt
     4501 gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa tggattattc
     4561 gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc tggcatttgt
     4621 ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc aagtagctgc
     4681 tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa tgcaagaatc
     4741 atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga ttctcctaaa
     4801 gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat gatcagagta
     4861 agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa acttgccacg
     4921 ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa gctaaatgta
     4981 tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc tggatcagat
     5041 agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag ttcatttgca
     5101 aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt catacccata
     5161 ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata agtggtatct
     5221 tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt tgtttctgct
     5281 agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat tccttcacca
     5341 tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc tgacacacca
     5401 ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc agcaggttca
     5461 ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt gatggcagtt
     5521 attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt tacattatct
     5581 aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa actgcctgga
     5641 tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt tttaaatgaa
     5701 gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc ttgattcgct
     5761 gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag aatggctgtg
     5821 aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt ccatgcacgt
     5881 ttcataaaat tcatattttg catccctcct agtagatgca tttgataata gacaacctat
     5941 cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt tctgtatata
     6001 taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac
     6061 cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac agagggattt
     6121 ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat ttgttgtata
     6181 ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt cgcaaaagat
     6241 ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca gaacgcggat
     6301 gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt tgcatatgct
     6361 tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta aacttggatg
     6421 tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg cggcgttcgt
     6481 gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat tttagattgt
     6541 gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc tgattttacg
     6601 tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg acatgcagat
     6661 gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt cgggacagag
     6721 ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa aggatttaag
     6781 gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt agtgagcttt
     6841 ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac aaaagaaggt
     6901 agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca
     6961 gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact aagtgaatcg
     7021 gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga tgaagtattt
     7081 gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa
     7141 cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt cttgactggt
     7201 caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca attaccaagt
     7261 gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga actgatcatt
     7321 ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc aaacggtaca
     7381 caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac gccaagcatc
     7441 gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg tggaacagtg
     7501 aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga cttgcaatta
     7561 atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta tcgtgaatta
     7621 gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat ctatattact
     7681 ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg aactacaagt
     7741 gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat tgttttacgt
     7801 gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat cagtcggcga
     7861 gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta cgtaaaagct
     7921 agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga gaaacattta
     7981 gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca attaggccgt
     8041 tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat ggaagctact
     8101 caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata gagtgctcgt
     8161 cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc gctttataat
     8221 aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga acgttattta
     8281 gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga agaatctttc
     8341 acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac tctcgtagtg
     8401 aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca cttagattac
     8461 aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat ggattttgta
     8521 ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa actcgcacat
     8581 cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt aattgtgtca
     8641 agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa gatcgtccat
     8701 cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca gagcagagac
     8761 ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc tgttacaatt
     8821 gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta tattttttca
     8881 ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt
     8941 aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg gaaaatggcc
     9001 aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta actgcagaag
     9061 aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata ggagatatga
     9121 ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt gctgattatg
     9181 cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat gcgtttccat
     9241 tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta gacggtatcg
     9301 ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa acgatttatc
     9361 atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa ttcgatattg
     9421 atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat gcagcaaagg
     9481 ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact ttccctgtca
     9541 ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga aaaataatga
     9601 gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga tttgatttta
     9661 gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat cgcaaaagaa
     9721 ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta
     9781 gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa aaaacatatt
     9841 gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt aggcggggcg
     9901 gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa acatgccatg
     9961 gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga agtccaaaaa
    10021 ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac gactgctgag
    10081 attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta tgatacattt
    10141 acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg
    10201 cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca gacagatacg
    10261 atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac
    10321 cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa atcgccaaac
    10381 cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt
    10441 gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt cgtttccgat
    10501 gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat gcagcttgtc
    10561 cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa tatccatacg
    10621 attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt cgtagtagca
    10681 ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga aatcatttct
    10741 gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg
    10801 aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg tccacgtgta
    10861 ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga aaacaccatc
    10921 gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa
    10981 tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc attatccaca
    11041 ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc gaactattac
    11101 gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa gggcttcgct
    11161 ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta gtcatcgtga
    11221 ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca gaaaaactga
    11281 tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa
    11341 aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct gaatctttaa
    11401 agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat acagggcgct
    11461 ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg agaaattatc
    11521 catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac
    11581 tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga agaataatcc
    11641 ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct tcgatcgttt
    11701 cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag cagccttcag
    11761 ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg gctaaacgcc
    11821 atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag
    11881 aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga aaaacagaat
    11941 gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc ttttcaagat
    12001 cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga agatgacatt
    12061 accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct tgttgatttg
    12121 cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag aagacggatt
    12181 agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga cagtgattaa
    12241 agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat
    12301 ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg gaccgatgaa
    12361 actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt cacattcttt
    12421 tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta
    12481 tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat tagttatttc
    12541 cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa
    12601 aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac agataaataa
    12661 tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag gtaataagat
    12721 gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc
    12781 tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga agattcgtgc
    12841 agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga ttatggtagg
    12901 tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt tttctttagc
    12961 attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc gagcaatcca
    13021 aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa gacacgtatt
    13081 atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc agctgttgcg
    13141 cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg tgaaatcagc
    13201 gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt tggaataaga
    13261 ggatgtgaca agaattttgt cacatcctct ttgttttgtt ctcataacct tttttcatat
    13321 acagttcatg aaccgctttt attaacttgc caaataacac tgtattgtgc gttgtatcca
    13381 gatagaaagt ctccgtttca tctgaaattt tctcatgctg atctgtaatg atcaaatcag
    13441 catgttctga atatgcggta attttaacag catctttgcg aaataactgc tgcaaatggc
    13501 tttgaagatg ataatttcct gtaaactgtt tggttatctg caaatggatg agtaactgct
    13561 ctttcgtttc tgtttgtagt aagtgttgga tcaattgact aaggtattga ctgatttggt
    13621 ttctttcccc attttttgaa aaaattgtat cgattttttc tcttaatttc ttttcctgta
    13681 taaaagaact agaatgattg gacgtggctt ctggaaagca aagtgtagaa aaactactgc
    13741 tggattcacc caacagttcg aaaaaaacaa ctgccaacac aagaaaataa aaataaattg
    13801 gtattttatt ttctaaatgg ttgagaggaa actgttgagt taagtaagtc atcaattgac
    13861 cgacttgtcg acaaataagg tttttgcttg tggaaaataa aaaaccaatc tcctgttttt
    13921 gtcgaaaaga gatttgattt ggaaaaaaac aatagaaaaa ataattaaag taggctctct
    13981 ctgtttctgt ctcttcacaa gggcgactca ctgaaaaaga agtaaagtat tctgaataat
    14041 catgtgtatg ctgcaataag tccatcaatt cagtgatttc agaagaagtt tttggaaaat
    14101 atgcctgttg tttgtttcgt aatatgaata aagttaacag caagtagata tttttttctt
    14161 gttttgaaat tttttctatt tgctcatctg tactgttcat ttttagtgca tttaattcaa
    14221 gtgaggaaaa tggccaatcg ttacctccaa atgtatctga aagaaaaaag taaagataga
    14281 gacggataga taactcattt ccttcaagag tacagcaatt ttttacagat aggatccgta
    14341 aatggaattt tattaaggaa tgattgagct gttttgtcag tttttcgaga taggagggcg
    14401 atatataaag ttgttcaagt aaatattttt ttggtacagc agtttgtgga taattgatta
    14461 gtaacatcaa atagttaaaa cgattggatt cttttaggta gcataatttt aacttagaaa
    14521 aaacagctaa ctttttagtt tgatcggttt ctttggtttg gatctctcct ttatgattag
    14581 aataaataaa ttcatcaatc aataaaaatt gcttgagtga tacatttatt tttcggatca
    14641 gttctttgat tgtccgtttt ggaatatgta agtcattgga taattcttgt aaagaacaat
    14701 tatttttttg aatgaccatt tgtagaacaa gtaactcttt ttttcttgct tcaattagca
    14761 taaatacctc ctttttattc tgtttttgta tatagttaga caaaaactgt gagtggaaag
    14821 tcatttcagt ctgtgttatt ctcacagagt
//
LOCUS       DACCXW010000067        14711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCXW010000067 DACCXW010000000
VERSION     DACCXW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.067"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(36..749)
                     /locus_tag="IVY71_001747"
     CDS             complement(36..749)
                     /locus_tag="IVY71_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7985801.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            924..2348
                     /locus_tag="IVY71_001748"
     CDS             924..2348
                     /locus_tag="IVY71_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7985802.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2647..3354
                     /locus_tag="IVY71_001749"
     CDS             2647..3354
                     /locus_tag="IVY71_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7985803.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3341..3664
                     /locus_tag="IVY71_001750"
     CDS             3341..3664
                     /locus_tag="IVY71_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7985804.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3753..4790)
                     /locus_tag="IVY71_001751"
     CDS             complement(3753..4790)
                     /locus_tag="IVY71_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7985805.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5111..6094)
                     /locus_tag="IVY71_001752"
     CDS             complement(5111..6094)
                     /locus_tag="IVY71_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7985806.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6509..7279)
                     /locus_tag="IVY71_001753"
     CDS             complement(6509..7279)
                     /locus_tag="IVY71_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7985807.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7272..8207)
                     /locus_tag="IVY71_001754"
     CDS             complement(7272..8207)
                     /locus_tag="IVY71_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7985808.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8523..10172)
                     /locus_tag="IVY71_001755"
     CDS             complement(8523..10172)
                     /locus_tag="IVY71_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7985809.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10289..12892)
                     /locus_tag="IVY71_001756"
     CDS             complement(10289..12892)
                     /locus_tag="IVY71_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7985810.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12904..13953)
                     /locus_tag="IVY71_001757"
     CDS             complement(12904..13953)
                     /locus_tag="IVY71_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP7985811.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13979..14293)
                     /locus_tag="IVY71_001758"
     CDS             complement(13979..14293)
                     /locus_tag="IVY71_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP7985812.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14309..>14711)
                     /locus_tag="IVY71_001759"
     CDS             complement(14309..>14711)
                     /locus_tag="IVY71_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7985813.1"
                     /translation="NASDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDRENHMTTGIG
                     KNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKGTNHLRIL
                     AELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4358 a         3053 c         2486 g         4814 t
ORIGIN      
        1 aaaaaataag ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca
       61 aaaacaaagt cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat
      121 agagcaacaa ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt
      181 gcgtcaaatt cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga
      241 cccagttcat ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct
      301 ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa
      361 cggcttcgta caatattata tacatagtct tttacactga tacttaattt atcagctact
      421 tttttcgatg gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca
      481 accacgctaa acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg
      541 acgacttcag tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct
      601 acgcctttct ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta
      661 ggaagtttct caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa
      721 atttccatat acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca
      781 tctatacttt aacataaata aaccgataaa atccacatca aaattataca cacaataaaa
      841 aagatatata tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag
      901 tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt
      961 tttggtgggg ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag
     1021 cagagactat ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg
     1081 attcctctat tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa
     1141 aaactgggca taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg
     1201 gtaccggaaa agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga
     1261 ataataacgg aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc
     1321 aagataaagg aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa
     1381 cttgttttaa agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg
     1441 ggcctatttt aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac
     1501 gcggagcgca agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta
     1561 aagaactagc tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc
     1621 gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga
     1681 gctttttaga tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag
     1741 aatataatca gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata
     1801 ccgctcaaat tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta
     1861 tggtggatac atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg
     1921 gacgacgaat gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt
     1981 gcatggatat caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg
     2041 gcgttgcgga tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga
     2101 ttgagtatat caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca
     2161 ttaaggggta tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa
     2221 accgatatgg gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg
     2281 gcgaatttta caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag
     2341 ttgaataata ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga
     2401 aataccaata acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact
     2461 gtgacaaatg tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt
     2521 taaactacgt ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa
     2581 atttagaata ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt
     2641 ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt
     2701 tttggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat
     2761 ccgctggtcg tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg
     2821 gtcagcatgc ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc
     2881 aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg
     2941 cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat
     3001 aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt
     3061 tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact
     3121 gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat
     3181 cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc
     3241 actttgattc tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt
     3301 gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac
     3361 attgctgact attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt
     3421 cttactgaaa aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat
     3481 cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc
     3541 gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc
     3601 aaaaagctta ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt
     3661 ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa
     3721 gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg
     3781 acttttccta atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca
     3841 ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa
     3901 gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta
     3961 gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac
     4021 ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt
     4081 tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct
     4141 aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt
     4201 tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa
     4261 tgcgctagtt gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt
     4321 ttcccgcggt tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa
     4381 gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca
     4441 agagcgacaa tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt
     4501 gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca
     4561 acaactttcc cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct
     4621 tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc
     4681 cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc
     4741 gacaaccctt cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct
     4801 ccttagcttc atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct
     4861 atttgttcca cttttattca tcataattat tctcttactt aaagtaaact atttgcactt
     4921 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc
     4981 aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt
     5041 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
     5101 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga
     5161 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
     5221 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
     5281 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
     5341 tttctgtatg ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc
     5401 caatcatgat gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg
     5461 ggatatctcc gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga
     5521 ttttagggat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
     5581 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
     5641 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg
     5701 cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt
     5761 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
     5821 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
     5881 tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat
     5941 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
     6001 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
     6061 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
     6121 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
     6181 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc
     6241 ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag
     6301 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     6361 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
     6421 agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
     6481 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
     6541 aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg
     6601 ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
     6661 tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa
     6721 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat
     6781 aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca
     6841 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
     6901 tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc
     6961 aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt
     7021 tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa
     7081 tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac
     7141 cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg
     7201 tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
     7261 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa
     7321 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
     7381 gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
     7441 tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct
     7501 aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc
     7561 tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct
     7621 aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc
     7681 cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa
     7741 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
     7801 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
     7861 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
     7921 tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt
     7981 gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta
     8041 ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat
     8101 agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca
     8161 cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
     8221 ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt
     8281 tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg
     8341 acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat
     8401 gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     8461 gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc
     8521 gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg
     8581 gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc
     8641 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
     8701 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
     8761 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
     8821 gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt
     8881 tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa
     8941 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
     9001 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
     9061 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
     9121 tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct
     9181 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc
     9241 aatattgcag cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt
     9301 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
     9361 atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa
     9421 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt
     9481 gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta
     9541 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac
     9601 ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc
     9661 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
     9721 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
     9781 tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc
     9841 tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta
     9901 gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga
     9961 tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag
    10021 ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt
    10081 tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata
    10141 ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc
    10201 tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa
    10261 aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat
    10321 catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa
    10381 gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa
    10441 caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta
    10501 attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt
    10561 tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta
    10621 accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa
    10681 atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat
    10741 gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc
    10801 caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact
    10861 cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct
    10921 tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa
    10981 tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt
    11041 tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga
    11101 gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt
    11161 ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt
    11221 aaacaactaa tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag
    11281 atagttctgt atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat
    11341 cgatcaaatg aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat
    11401 ctttaaaaga aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg
    11461 tttctaattt ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat
    11521 ttacatttat ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg
    11581 tagcaatcat ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat
    11641 tatagggact acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg
    11701 atccactata attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta
    11761 atgcatcagc catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt
    11821 ctttgattcg atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat
    11881 catgagcctg gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat
    11941 ggataccatt tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt
    12001 ccaatttaaa attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac
    12061 gagagtaaac cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga
    12121 taacttctac ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca
    12181 acttctctac gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta
    12241 ctaaaatatc tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt
    12301 cttttgcaaa ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac
    12361 caaatggaaa attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag
    12421 gagatggaac catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta
    12481 cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta
    12541 ccatcattgg atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta
    12601 aaattgattc agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata
    12661 gttgtcctgt tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat
    12721 actcatcgac gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca
    12781 attcatctag aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa
    12841 aataccattc tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt
    12901 ctcctacttt ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt
    12961 tagtgcattg accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa
    13021 aggtatattc gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac
    13081 accaaaaaat ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac
    13141 tcgtaaagga tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat
    13201 aatcatagct gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat
    13261 tgttgctaaa aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg
    13321 tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg
    13381 tcctcccata tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc
    13441 tcctgacatg ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc
    13501 tagtacatac cataatatga ttccaacaac taatgtagta acaaaaggaa tcagcataat
    13561 tgattttaga ctaataactt gcatcggtac ttttactttt ttcaatgcat tagctaaata
    13621 gccacaagct aatccagcaa ccattcctcc aataaatccc ataccaagat tttgacacat
    13681 catcccagtg ataaaagctg gtgctagacc gggacggtca gcaattgaat atgcgatata
    13741 ggctgaaatc atcggtacga taaactgtaa tcccgtctga ccaatcattc gcaggactcc
    13801 ccagaatgta tccgtctggt cccaaacacc tgtgccacca aaaatgactg caatagaagt
    13861 tagtaaccct gctgctacga caagcgggat catataggaa accccacttt ggaaatgatt
    13921 tagaatttct cttccttgtt tcttgaattc cattagtaac tcccccacga tatatttttt
    13981 atttgttaat taatgcaatt gcttcatcaa tcgtctcttc accatctttt actacctctg
    14041 aggtccctac tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa acatttgtat
    14101 ctactgcaaa tatgacaaca tctgcctcgg aaacatccga aattgaaagt ttgttttcta
    14161 tccccatagc cccctgtgtt tctactttaa tatctaaccc tttcttcttc gcaaactttt
    14221 ctaaattagc ttttgccata taagtatggg caatccctgc gatacatgct gttactgcta
    14281 ctatttttgt catatgaaat tcctcctttt aaaatgatag agcttttacc aattcttttt
    14341 tatttctcga atcttttatt cttttaacaa actcgtcatc catcaatctt ccagataatt
    14401 ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc ttttattgct aacaaaaaga
    14461 cgagatcaac tggttgatca tctaatgcgc cccattcaac agaccgcttt aacttggcta
    14521 cagccactgt tgattcaatt actgctaggc ttttgccatg aggaatggcg atatttttcc
    14581 caattcctgt ggtcatatgg ttttctctgt caaatacgtc tttttgaaat tgtttgtggt
    14641 cagacacaaa cccattagtc tctaacaatt tcgataattt ttcgactaca ctttttttat
    14701 cagatgcatt t
//
LOCUS       DACCXW010000068        14476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCXW010000068 DACCXW010000000
VERSION     DACCXW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.069"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..601)
                     /locus_tag="IVY71_001760"
     CDS             complement(26..601)
                     /locus_tag="IVY71_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7985814.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="IVY71_001761"
     CDS             complement(606..1580)
                     /locus_tag="IVY71_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HAP7985815.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="IVY71_001762"
     CDS             1863..2831
                     /locus_tag="IVY71_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7985816.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="IVY71_001763"
     CDS             2928..3221
                     /locus_tag="IVY71_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7985817.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="IVY71_001764"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="IVY71_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7985818.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3687..4433
                     /locus_tag="IVY71_001765"
     CDS             3687..4433
                     /locus_tag="IVY71_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7985819.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="IVY71_001766"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="IVY71_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7985820.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="IVY71_001767"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="IVY71_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7985821.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="IVY71_001768"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="IVY71_001768"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7985822.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="IVY71_001769"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="IVY71_001769"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7985823.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="IVY71_001770"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="IVY71_001770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7985824.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="IVY71_001771"
     CDS             9170..9712
                     /locus_tag="IVY71_001771"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7985825.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="IVY71_001772"
     CDS             9735..11291
                     /locus_tag="IVY71_001772"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7985826.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="IVY71_001773"
     CDS             11305..12207
                     /locus_tag="IVY71_001773"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7985827.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="IVY71_001774"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="IVY71_001774"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7985828.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="IVY71_001775"
     CDS             13650..14072
                     /locus_tag="IVY71_001775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7985829.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4780 a         2608 c         3113 g         3975 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt cctttt
//
LOCUS       DACCXW010000069        14397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCXW010000069 DACCXW010000000
VERSION     DACCXW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.070"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..341)
                     /locus_tag="IVY71_001776"
     CDS             complement(<1..341)
                     /locus_tag="IVY71_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7985830.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(341..571)
                     /locus_tag="IVY71_001777"
     CDS             complement(341..571)
                     /locus_tag="IVY71_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7985831.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            858..1196
                     /locus_tag="IVY71_001778"
     CDS             858..1196
                     /locus_tag="IVY71_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985832.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1401..1640)
                     /locus_tag="IVY71_001779"
     CDS             complement(1401..1640)
                     /locus_tag="IVY71_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7985833.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1616..1963)
                     /locus_tag="IVY71_001780"
     CDS             complement(1616..1963)
                     /locus_tag="IVY71_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP7985834.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2695..3276)
                     /locus_tag="IVY71_001781"
     CDS             complement(2695..3276)
                     /locus_tag="IVY71_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7985835.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3459..4085)
                     /locus_tag="IVY71_001782"
     CDS             complement(3459..4085)
                     /locus_tag="IVY71_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7985836.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4107..5438)
                     /locus_tag="IVY71_001783"
     CDS             complement(4107..5438)
                     /locus_tag="IVY71_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP7985837.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5593..5994)
                     /locus_tag="IVY71_001784"
     CDS             complement(5593..5994)
                     /locus_tag="IVY71_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7985838.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6080..6286)
                     /locus_tag="IVY71_001785"
     CDS             complement(6080..6286)
                     /locus_tag="IVY71_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985839.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6381..7883)
                     /locus_tag="IVY71_001786"
     CDS             complement(6381..7883)
                     /locus_tag="IVY71_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7985840.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7901..8356)
                     /gene="argR"
                     /locus_tag="IVY71_001787"
     CDS             complement(7901..8356)
                     /gene="argR"
                     /locus_tag="IVY71_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7985841.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8454..9071)
                     /locus_tag="IVY71_001788"
     CDS             complement(8454..9071)
                     /locus_tag="IVY71_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985842.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9292..10665
                     /locus_tag="IVY71_001789"
     CDS             9292..10665
                     /locus_tag="IVY71_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7985843.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10740..11852
                     /locus_tag="IVY71_001790"
     CDS             10740..11852
                     /locus_tag="IVY71_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7985844.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11924..13561)
                     /locus_tag="IVY71_001791"
     CDS             complement(11924..13561)
                     /locus_tag="IVY71_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7985845.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13729..14337)
                     /locus_tag="IVY71_001792"
     CDS             complement(13729..14337)
                     /locus_tag="IVY71_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7985846.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3906 a         3011 c         2545 g         4935 t
ORIGIN      
        1 taaaacatat actgtataat ctgatcaatt ttagccatgt cttgcaatgg taagttttct
       61 atttttttga gttttcgttc cttaaaatca agagatttta attgtgtaat aattacttgt
      121 ccttttatat ctagctcttc aggtaatgag taacgtgtcg gtaaattctt tatagtggaa
      181 gtaattggac atatgaccga aaacattgtt ttacggttaa attcataacg agagacgact
      241 agtcctggtc gtcttttttg aatttctttt cctacggagg gatcaaaatc aatccataca
      301 atatcacctt ttttaggaat ataaattctt tcttctgaca ctataaaatt tctctccctt
      361 caggagttaa atcttcccat tcgtctttct cataatactc ggcctcttcc ccaccactaa
      421 atggatcttc aatttttgga actaacgtaa ttgttccatc atcagaatac acaacgatat
      481 attcttggtt ttctgaaggt ttctttccgt tatcagaagg taaagttacc actacagaac
      541 ttccttgtaa tctagatttt gtaacaagca ttatcatcac tcccttaata aaagtatact
      601 atataacact aagtaacgca aaattcccct ttgtaaacta tctagaaaat tgttttgttt
      661 aaagtatatc ttattggata taaatagaaa taaattgtag ccagttcatt ttttctttaa
      721 ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc aaatactata
      781 gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa atttcaaaga
      841 caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc ttttgctctg
      901 acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca actgttcgga
      961 tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact agacaagact
     1021 ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt ttacatgatc
     1081 caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga gaatatacaa
     1141 ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag tcataatgat
     1201 aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca aatagaagct
     1261 atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt atgagcattt
     1321 cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt tgccgctctt
     1381 tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat ctgctgagta
     1441 atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc ttcagtcaag
     1501 acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt agtcattatg
     1561 attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat atacactatt
     1621 ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcatt gggaaaacac
     1681 aacgattttg caaaaatggt tgatgaaagg tctttttttc ttctactgtc tctgcacggg
     1741 cattaatcat caattgtcct tttttaaatc cggtaaatcc ccacttcatg atttccggag
     1801 tgaggatgtt ttcttcctct tttcttaacg tcacaacaag atttgacgga aaaacttctc
     1861 cagctgctaa ttctgctgcc tgcttcggtt gtttcgtctg ggcttctcga taataattct
     1921 gaaggagctc tgactctaga tcaaaaaaat aacgaccaca catcctaatc cctcccaatc
     1981 tttttttcta tcatacactg caacaagaag ttcgcaccaa caaaacgctt tcaatttttg
     2041 tagaactaaa aaagttgtga caagtgacaa actctccagt gaatggtatt ctcctgccaa
     2101 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgcttttt gaaattgttc
     2161 cttgtgactc ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg ttcaaaagcc
     2221 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagctatttt cacaaaattt
     2281 ttcttaatac tcgaaactaa gcgacttttt cacaacctct tccttaaacg gtgttcaaaa
     2341 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagcagcta tttttatttg
     2401 gctgttctta ttacttgatg cagggcagct ttttcacaac ctctccacta aacggtgttc
     2461 aatcagctaa ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg
     2521 tttgtttctc taatactcag gtcaaagcgc tgatttcaca acctctaata cacggtgttc
     2581 cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaatttgaga agcaattttc
     2641 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcta attattaaag
     2701 aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc taagtagtga
     2761 tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa tcaacgcttt
     2821 ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca ggatattttg
     2881 tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt attcttcatc
     2941 cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag tgatctgtgt
     3001 ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg gctgttttcc
     3061 agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg catggatcac
     3121 cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt ttcctgtatc
     3181 agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat aaataagatc
     3241 tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt ttcatttctc
     3301 atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt aaaaaagact
     3361 gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc tagatgttca
     3421 ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg ctctaatact
     3481 tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc tgcatccatt
     3541 gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa tccactctct
     3601 accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc aagaattact
     3661 gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg tccaaagatt
     3721 ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc ttcgataaaa
     3781 tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa catctgctgc
     3841 tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa
     3901 taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac catttcaatc
     3961 atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa gccaccgcct
     4021 gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt tttattattg
     4081 aacatggtgg attctccatt cgttatttat tttaatacat catttagagc ttcactcatt
     4141 gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc attcatagct
     4201 agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc ccctagaatc
     4261 aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc taatacttgt
     4321 gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc ttgtgctttg
     4381 gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac actgtaaggc
     4441 acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc cataatgatt
     4501 cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc
     4561 caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc gcgatctgtt
     4621 agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct tccagttgca
     4681 gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa ggtgacggca
     4741 gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa ttcgattccc
     4801 gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg tggaagaaat
     4861 tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat tgatgcaaat
     4921 tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg ttcctgatcc
     4981 atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat tggtaaaatg
     5041 attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac tttttcacca
     5101 ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc catcactgtc
     5161 ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc cgtcaatttt
     5221 tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa aatcaatgat
     5281 ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt tgacttttca
     5341 acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt accgcccttt
     5401 ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct tcttttttac
     5461 ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa tcatctgctt
     5521 tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc tggttcgatt
     5581 tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag ttgctttgga
     5641 gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt cgctgatgcc
     5701 atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta aattataaag
     5761 agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga tcatatgttg
     5821 taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg gatttgttcc
     5881 ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg gtgcctcgac
     5941 ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt tcattgtact
     6001 gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac aagtaaggcg
     6061 ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact atttttccaa
     6121 aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa
     6181 tcttttctac ataatctttt atatgattga ttttaagata catatatacc agaaagttga
     6241 ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt tcctcctttt
     6301 gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt ccaagcaact
     6361 agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga aaaatgtccg
     6421 aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc attttctatc
     6481 tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc gtttccgtat
     6541 ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt catccctttt
     6601 tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc gcgtggttca
     6661 tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa ctctttgatt
     6721 tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag gatctttgcg
     6781 atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg tccgcttgtt
     6841 tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt attgattaca
     6901 tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat gaatctttgt
     6961 agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc ttttaatacg
     7021 attgtactta atactgcgat caaattattt cgattgatca ctgttccaga agcactgaca
     7081 actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa
     7141 acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat atgattagga
     7201 aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa gctattcaat
     7261 actttttcag caaagaatcc accagcacca tttccggcat caacaatgat tttccaacca
     7321 tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac taaatcttgc
     7381 gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct ttttattttt
     7441 tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc tgaggtaaag
     7501 aattttattc cattataata atagggaaga tggctagcag tcagcataac cgcagcatcg
     7561 cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc taattcgaaa
     7621 tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt cagtgctggt
     7681 ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg atcggttgat
     7741 ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac catgtctgtc
     7801 gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc cgatccattt
     7861 tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc attttttcca
     7921 gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg aatgtatcta
     7981 caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa ttcgttacca
     8041 catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac tggatacgca
     8101 aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca gatggctggt
     8161 taaataatgt atagcgtact attttgtttt cattttgact ttttactaac ttcatttctc
     8221 gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa
     8281 gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc
     8341 gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag ataagtaata
     8401 tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg ttgctaacct
     8461 ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt tcttggatcg
     8521 ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt tgcgatgaat
     8581 gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg taagatttgg
     8641 gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc ttccggaagt
     8701 ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg
     8761 atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa atacttttct
     8821 tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc aggaatcagc
     8881 caattttttt taggcgacat gcgtattgca tagctgactg catatagaca ggctactctt
     8941 cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa tgatttttta
     9001 ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt acgccattca
     9061 attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat catacctctt
     9121 tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag aataagtgaa
     9181 gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg tttctatttt
     9241 tataatagaa atcactctct ttttttacga aattttttag aggtgatgta aatgacaaaa
     9301 caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc ttttgcgggc
     9361 gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca gtttgattta
     9421 acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc gatcattgtt
     9481 ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt agcttcaaat
     9541 ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat ggtcctattg
     9601 tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat gttccatatt
     9661 atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg tactttgaca
     9721 acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc acattttaca
     9781 tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat tatcggctgg
     9841 tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat aagcgtttta
     9901 ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg aacagtttcc
     9961 agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta tcgaagtaaa
    10021 aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt ccgcttgttt
    10081 ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt cttgccgaat
    10141 ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat gcttcccggc
    10201 gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca atatggtccg
    10261 aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc attattccta
    10321 ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta tatgatcggt
    10381 atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa tgaagaacta
    10441 caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc tgtggctact
    10501 tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa ctatgaagtg
    10561 tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc
    10621 tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc atatacggca
    10681 ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta
    10741 tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc agttacttag
    10801 atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca actaaagcta
    10861 gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg
    10921 ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt gatcaaacga
    10981 tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct gaattggctg
    11041 ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa tggctttcac
    11101 ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg cgtatcagtg
    11161 gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt ttagcacaaa
    11221 aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat tcttggctca
    11281 tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct ggcccggcac
    11341 ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa aaagggcagc
    11401 tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct atgcttgacg
    11461 gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct acccatgaaa
    11521 acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg actaagagta
    11581 cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag ttccgtgtag
    11641 gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca gatgatccaa
    11701 gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct tatatcggct
    11761 atctattagc caacttctta aatactggtc attggcctac ggaagattat caaaaggaca
    11821 tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag taaggatccc
    11881 gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc aatttctgaa
    11941 tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct atctgagctt
    12001 caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag gacgtatcct
    12061 caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat ggaccaaacg
    12121 acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt
    12181 tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt tgctgtttca
    12241 aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg aaaggattag
    12301 caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt tccgcgtctg
    12361 atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca ttgatcttga
    12421 taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc ttttcgctaa
    12481 gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca tttccgtttc
    12541 gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg cccacattct
    12601 gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt gttttatcaa
    12661 agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg aaagcatcgt
    12721 catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc gttgattttc
    12781 cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta gcatattgtt
    12841 tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa gcaagcgtca
    12901 aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct gtattatgga
    12961 gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg ggatcacaaa
    13021 agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct aagatcttcg
    13081 aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat ccttctggaa
    13141 tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact tttgtatggt
    13201 aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg tatttgcttt
    13261 gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc acaatgcttc
    13321 catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca gcatgtgaga
    13381 ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa gctgtcttcc
    13441 tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac
    13501 tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct tttgtactca
    13561 tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt attcgagttt
    13621 ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa aaaaaccgag
    13681 aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt agtaccagcc
    13741 gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg ttacatattg
    13801 ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg aagcatctaa
    13861 ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg attctttttg
    13921 tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg gggcagcagg
    13981 agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt
    14041 tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat catttgaagt
    14101 tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg aatttttttc
    14161 agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct tagataatga
    14221 atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc ctacaaacat
    14281 tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg aattcatatt
    14341 aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat catttag
//
LOCUS       DACCXW010000070        14276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCXW010000070 DACCXW010000000
VERSION     DACCXW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.071"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..202)
                     /locus_tag="IVY71_001793"
     CDS             complement(<1..202)
                     /locus_tag="IVY71_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HAP7985847.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLT"
     gene            327..932
                     /locus_tag="IVY71_001794"
     CDS             327..932
                     /locus_tag="IVY71_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7985848.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(998..1630)
                     /locus_tag="IVY71_001795"
                     /pseudo
     CDS             complement(998..1630)
                     /locus_tag="IVY71_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
     gene            1919..2311
                     /gene="merR"
                     /locus_tag="IVY71_001796"
     CDS             1919..2311
                     /gene="merR"
                     /locus_tag="IVY71_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HAP7985849.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYD
                     DVKRVGFIKRIQELGFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKR
                     IETMLDDLKQRCPDEKQLHSCPIIETLT"
     gene            2340..4235
                     /gene="merA"
                     /locus_tag="IVY71_001797"
     CDS             2340..4235
                     /gene="merA"
                     /locus_tag="IVY71_001797"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HAP7985850.1"
                     /translation="MNKFKVNISGMTCTGCEKHVESALEKMGAKNIESSYRRGEAVFE
                     LPDDIEVESAIKAIADANYHPGEAEEFQSEQKTNLLKKYRLNVEGMTCTGCEEHIAVA
                     LENAGAKGIEVDFRRGEALFELPYDVDIDIAKTAITDAQYQPGEAEEIQVQSEKRTDV
                     SLNDEGNYDYDYIIIGSGGAAFSSAIEAVTLNAKVAMIERGTVGGTCVNVGCVPSKTL
                     LRAGEINHLAKNNPFVGLHTSASNVDLAPLVKQKNDLVTEMRNEKYVNLIDDYGFELI
                     KGEAKFVNENTVEVNGNQITAKRFLIATGASSTAPNIPGLDEVDYLTSTSLLELKKVP
                     NRLTVIGSGYIGMELGQLFHNLGSEVTLIQRSERLLKEYDPEISEAITKALTEQGINL
                     VTGATYERVEQDGDIKKVHVEINGKKRIIEAEQLLIATGRKPNTESLNLHAAGVEVGS
                     RGEIVIDDYLKTTNSRIYSAGDVTLGPQFVYVAAYEGGLAARNAIGGLNQKVNLEVVP
                     GVTFTSPSIATVGLTEQQAKEKGYEVKTSVLPLDAVPRALVNRETTGVFKLVADAKTL
                     KVLGAHVVAENAGDVIYAATLAVKFGLTVGDLRETMAPYLTMAEGLKLAVLTFDKDVS
                     KLSCCAG"
     gene            4395..5717
                     /locus_tag="IVY71_001798"
     CDS             4395..5717
                     /locus_tag="IVY71_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7985851.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKL
                     YKHSSRNQLIMDLPIRLKRVGLQLNRRRYKCRECGSTFWERLISVDEKRSMTKRLLKS
                     IQEQSMSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRP
                     RLVLTNIERRTIYDIKPNRNKETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNPILPH
                     AKVVVDKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFL
                     LDTWLGNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQWRHRCMSSNSKDAYKDLVR
                     AVDNWHVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKR
                     KPRFNSSAFNKAMLYDTFNWYEVNDHDITDNFGVDFSTPIKNLEKGDL"
     gene            complement(5844..7349)
                     /locus_tag="IVY71_001799"
     CDS             complement(5844..7349)
                     /locus_tag="IVY71_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepS protein"
                     /protein_id="HAP7985852.1"
                     /translation="MKVSDVISVILKSGIREYKCLNSKIKPIGYKAEGKKGAIFGFRS
                     KKLMTESRGIVLTSQEGLAENEDKFTHWTPNVYSYGAYADEKRIIVKGHNERNLQQIN
                     TFVIDFDRLTGEKLDSQMILDAAIDLELMPTLILETPGGFQAYFILENAWYISSKNNY
                     QSIEVAKRVSENLRKAFAEELPSVDLGCNHFGIARIPRTDNIVYYYPALTHDIQQLIQ
                     WSMKYEPAKTYKKPLLSLVKNKYKQVNEMWYRQLISNPKIVGAKGIMGRNNVIFTLSL
                     ANYASGVDLEDCLNEMDLFNSSLERPLKDRERVRIVKSAYSGKYRGASKTYVQELVSN
                     WGLESLSEEKMFTQQRNTWYKFKKERHKRKKSHLSEWKEDILAYLETQCYRYRPEVRL
                     KKTDLQAAITFNGQSIPKRSLDRALKELMAEGKLFVQIKAGRNGGLVVATRKALIRTV
                     IQVKQQVKSAYKQGIKTFFKEADMLVRIFREPDKYSQRVDHYKQLNLWNTG"
     gene            8053..8943
                     /locus_tag="IVY71_001800"
     CDS             8053..8943
                     /locus_tag="IVY71_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7985853.1"
                     /translation="MANTIIFGQQKGGVGKTTDTCMITLVAAMLMKKRTILIDIDLQA
                     NATNFMTKTFGVETVPQTLMSALENGDLRPAIVNLKENLDLIPAGYDMRLYTEFLTEN
                     FDNTIDRTFYLKNLIEEIKDDYDYIFIDIPPSTDLKVDNAMVACDYLVVVQETQQFSF
                     EGSQRLIFDYIQTLVDDFGSDVPIQIAGILPALLQQKRPMHQKLVEETIDTFGRDNVF
                     NTIIQNHARLEWYPRIGFQFQDYHDKKMIALFADIFCELEERITLYKSNGDIQDYFYT
                     PRYILDGKLTSLGKEISLNV"
     gene            8936..9220
                     /locus_tag="IVY71_001801"
     CDS             8936..9220
                     /locus_tag="IVY71_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985854.1"
                     /translation="MSSLINRKIPQGATANKEADTKRKVTIQKKSSKKILASERKNIK
                     VSPSTFDLIKTISTMKSYKNYEFIDKAVESYIQNNLTEREQRILKNLISK"
     gene            complement(9253..9492)
                     /locus_tag="IVY71_001802"
     CDS             complement(9253..9492)
                     /locus_tag="IVY71_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010621028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985855.1"
                     /translation="MEQSDNVPLDRHKCKEHVFKDKRFVEKESESLTLLGYKIVVDTV
                     TGVNYLETPKGGITPLLDKNGKICIDKKKSINKKG"
     gene            complement(9888..10085)
                     /locus_tag="IVY71_001803"
     CDS             complement(9888..10085)
                     /locus_tag="IVY71_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7985856.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYKQKQGEYVAITIIKE"
     gene            complement(10119..10418)
                     /locus_tag="IVY71_001804"
     CDS             complement(10119..10418)
                     /locus_tag="IVY71_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985857.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(10459..11076)
                     /locus_tag="IVY71_001805"
     CDS             complement(10459..11076)
                     /locus_tag="IVY71_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7985858.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            complement(11401..11796)
                     /locus_tag="IVY71_001806"
     CDS             complement(11401..11796)
                     /locus_tag="IVY71_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985859.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            complement(11876..14227)
                     /locus_tag="IVY71_001807"
     CDS             complement(11876..14227)
                     /locus_tag="IVY71_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7985860.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         4699 a         2556 c         2293 g         4728 t
ORIGIN      
        1 tggtaagatc taatataaaa gttcctaaaa aacgtagtga gactaactgt agtgcaaaac
       61 ctaatctagt agctggtcgt cgtatagtct ccatcaattc taaatctttt tcatgcaaat
      121 aaaaaaattg gtttaattgt tctgtactag gttctcctaa ataaatgcca aatgcttttt
      181 tgttactttt gatttcatcc atttaaaccc tccatgaagt aaattttaag tctacataaa
      241 atttattttt aaaaatgcta tgatagcagt aaaactacaa gtaaatttta catgtaaatt
      301 atatcacttt taaaaaggag ttttttatgg gaaaaattgg ctatattcga gtatcttcac
      361 aagatcaaaa tttggatcga caattggcaa tgatggaagg acttaacatt gataaattat
      421 tccaagaaaa agccagtggt aaagatactg agcgtcctga gtttcaaaaa ttactaaatt
      481 atatccgtga aggtgattgc gtcatcgtta catcactaga tcgtttagga agagattatg
      541 aagatataaa aaatacagtt gcttttatga aacagaaaaa agtagcgtta aaaatattag
      601 atgcacagtt tttagatttt aatactggaa atgaactcct ggataccgct atgttcgata
      661 tgtttttatc actgctaagt tatattgcac aaaacgagcg tgagaaaatt cgtgaacgtc
      721 agcggcaagg agtattattg gctcaaaaag ccggccgata taagggacga ccaatcgagt
      781 attctattaa ctcaacagat ccgcaaaaac gcttagtttt taaaaccgtg gtggacatgt
      841 taaagcaagg cattccagtt gcagagattg caaaagaaaa cgggatctct cgaccaacta
      901 tttataaaat aaagaaagcg aatgatttgt agaaatatac tacattcatt cgcttttcta
      961 ccgcactatt tcgtttctat gttccatgta ccccgatata gggtccatta taaatcacac
     1021 tatcagagtt cgtaccatat ctatctcctt ctttttcaac aacttcctgt ttttgaggaa
     1081 aaaatgctgt attaattagc atagaagtga aatatgggat tggcgtactc aaattaataa
     1141 ccaaagtatt cttatcctta gcataaactc ccaacttatc tggtttcata gttcctgaga
     1201 ttatttcttt accattttta atagattcaa aaaaaccaat atttggtgaa tcttgattac
     1261 ttaacgttct tttccacgaa taaacatagt catctgcaac aacaggcgta ccattactcc
     1321 acattgcatc ctttctcaat cgaataacgt acttaagtcc atccttagag atttctggaa
     1381 ggccattagc tcctccaggt acaatttcac ctttagcatt aagtttatat aatccttcca
     1441 ttacgttatt taaaacaagt tggcttgtag ggtcgatagc taacttcgga tctaagcttt
     1501 gtactgtagt agaaacagca aaagtataag ctttggtttc ttttgctgtt tcttgagact
     1561 tttccccaca accagtaata attattccta acataagaat tgttaaattc tttccccaag
     1621 ttcttttcaa aaaaacaccc ctattcaatt tcttatctga ttgttctagc aacacttagc
     1681 ttaattatac tgtaaaaaaa acgggtcttc ggaatatata gtggaattac tttcttaata
     1741 gtttaaaaac aactggtgtt taaatcggat tttctggtgg gatgttagga taatcaatag
     1801 tattatataa aagtatcaat cacatacgag tgatactttg ggaaaaaaat aaaatagtcc
     1861 aatttacttg aacgtgtact atggtacacg gtttataata ttcatggggt gaaataagat
     1921 gatttatcgc attagtgagt ttgcagataa atgtggagtt aataaagaaa cgatcagata
     1981 ttacgagcga aaaaatttat tacaagaacc tcaccgaacg gaagctggtt atcggatata
     2041 ttcatatgat gacgttaagc gtgttgggtt tattaaacga atacaggaac ttggtttttc
     2101 tttaagcgag atttataaat tacttggtgt tgtagataaa gatgaagttc gttgtcaaga
     2161 tatgttcgaa tttgtttcta aaaaacaaaa ggaagtgcaa aaacaaatag aggatttaaa
     2221 acgaattgaa actatgttag acgacttaaa acaacgatgt ccagatgaaa agcaattaca
     2281 ttcgtgtcca ataatagaaa cattaacatg agagattaat taacgaaagg agctttttta
     2341 tgaataaatt taaggtaaac atttcaggaa tgacttgtac gggttgtgaa aaacacgtag
     2401 aatcagcact tgaaaagatg ggtgctaaaa atattgagtc tagttatcgt cgtggtgaag
     2461 cagtatttga actgcccgat gatattgagg ttgaaagtgc aataaaggcg attgctgacg
     2521 caaactatca cccgggcgaa gcagaagaat ttcaatcgga acaaaagacg aatttattga
     2581 aaaaatatcg gctaaacgtt gaaggaatga cctgcactgg ttgtgaagaa catattgcgg
     2641 ttgctcttga aaatgcaggt gcaaaaggga ttgaagtaga ctttcgtcgc ggagaagcac
     2701 tgtttgaact accgtatgat gtagacattg atatcgcgaa aacagcgatt actgacgcac
     2761 aatatcaacc gggcgaagca gaagaaatac aagtgcaatc ggaaaaaagg acagatgtaa
     2821 gtttaaatga tgaaggtaac tatgattatg attacatcat catcggttct ggtggagctg
     2881 ccttttcatc tgccattgaa gccgttactt tgaacgcaaa agtggctatg attgagcgtg
     2941 gaacggtggg tggaacttgc gttaatgtcg gatgcgttcc ttctaagacc ttattaagag
     3001 caggggaaat caatcatcta gcaaaaaata atccatttgt gggattacac acttcggctt
     3061 caaatgttga tttagcgcca ttagtaaaac aaaagaatga tttagtaacc gagatgcgaa
     3121 atgaaaaata tgtgaattta attgatgatt atggttttga attaataaaa ggtgaagcaa
     3181 aattcgtaaa tgaaaataca gttgaagtaa atggcaatca aatcacagcc aaaagatttt
     3241 taatagctac aggtgcttct tcaactgcac ctaatattcc cggattagat gaagtagatt
     3301 atttaacaag cactagctta ttggaattaa agaaggttcc aaatcgtctt accgtaattg
     3361 gttcaggata tatcggcatg gaattaggac aactatttca taacctcggg tcagaagtca
     3421 ctttgattca aagaagcgag cgtctattaa aagaatacga tcctgaaatt tcagaagcca
     3481 ttactaaggc cttaacagaa cagggaatta atttagtaac aggtgcaacc tatgaacgag
     3541 ttgagcaaga tggagacatt aaaaaagttc atgttgagat aaatggtaaa aagcgaatta
     3601 ttgaagcaga acaattgcta attgccactg gaagaaaacc aaatacagaa tcattaaact
     3661 tacatgcagc aggcgttgaa gttggttccc gtggtgaaat tgtcattgat gattatctta
     3721 aaacgaccaa ttcccgaatt tattcagctg gagatgtcac tctcggtccc caatttgttt
     3781 atgtagctgc ttatgaaggt ggacttgctg ctcgtaatgc aatcggagga ctaaatcaaa
     3841 aggtcaattt agaagtggtt ccaggcgtta cgtttacttc tccatcgatt gcaacggttg
     3901 gtttaacgga gcaacaggca aaagaaaaag gatatgaagt gaaaacatcg gtattgccgt
     3961 tggatgctgt tccaagagcg ctcgttaatc gggaaacaac aggtgttttc aaattagtgg
     4021 cagacgcgaa aacattgaaa gtgttagggg cgcatgtagt ggcagaaaac gcaggagacg
     4081 taatttatgc agcaacatta gctgtgaaat tcggtttaac tgttggagat ctgagagaaa
     4141 cgatggctcc atatctaaca atggcagaag gattgaagct ggctgtccta acttttgata
     4201 aagatgtttc gaaattatct tgctgtgcag gctaagggaa cttttttaca actccctatt
     4261 caagtgaacc agctaatgct ttaagggatt ttctaagtgc atttgtggtc acaaaaataa
     4321 tttaacactg atgatttcga catcaaatct ataattgata ccttaacacc gcagagtaat
     4381 aaaggatgaa taatatgtca gacttattat ccctaccaga cattaaaaca atagagccgc
     4441 cacaagaaaa tgaaaccgat atgatgttta aagttgaagc agtcggacca cctgaacgtt
     4501 gtcctgaatg tggttttgac aagttgtaca aacacagttc aagaaatcaa ctaattatgg
     4561 atttgcccat tcgtttaaag cgagtgggct tacaattgaa ccgtagacga tacaagtgtc
     4621 gtgaatgcgg atctaccttc tgggaacgcc taatatctgt agatgagaag cgtagtatga
     4681 ccaaaaggct tttaaagtcc attcaagagc aatccatgtc taagaccttt gtagaagtcg
     4741 cagaaagcgt tggtgttgac gagaaaacca ttaggaacgt ttttaaggac tatgtggcac
     4801 tcaaagaacg tgaataccag tttgaaactc ctaagtggct tgggatagac gagatacata
     4861 ttatccgtag acctcggctt gtattgacta atattgaacg caggactatt tatgacatca
     4921 agcctaaccg taacaaggaa acagtcatcc aacgtctttc agaaatcagt gacaggactt
     4981 acattgagta cgtcacaatg gatatgtgga agccctacaa agacgcagtg aaccctatcc
     5041 ttccacacgc taaagttgtc gtagataagt ttcatgtagt tagaatggct aatcaagcct
     5101 tagataacgt caggaagtct ttgaaagccc atatgagcca aaaagaaaga cgtaccctta
     5161 tgcgtgaaag gtttatcctt ctaaagcgta aacacgattt aaatgaacgt gaatcattcc
     5221 tcttagatac ttggttaggt aatcttcctg ctttaaaaga agcctatgaa ctcaaagaag
     5281 agttttactg gatatgggat actcctgatc cagatgaagg tcatcttcgt tatagtcaat
     5341 ggagacaccg ttgtatgtcc agcaactcta aagacgcata taaagacctc gtgagagccg
     5401 tagacaactg gcatgtcgaa atattcaact actttgataa aaggctcact aatgcttata
     5461 cggagtcaat taacagcatt attaggcagg tagagcgaat gggtagaggt tactcgtttg
     5521 atgccttacg agccaaaatc cttttcaatg agaagctcca taaaaagcgt aagccacgat
     5581 ttaattcaag tgctttcaat aaagctatgt tatacgatac tttcaattgg tatgaagtga
     5641 atgatcacga cattacagac aactttggtg tcgatttttc cacacctatt aagaatttgg
     5701 agaagggtga tttataagcc cttttccacc ataaaatccg aatacccaaa aaaacaaaaa
     5761 tatacagaat taggataata actaacaaaa acgaaaacaa gtataaaaga aatataatgt
     5821 ataggcactt gacatgtttt attttatcct gtattccaca aatttaattg cttataatga
     5881 tctactcttt gactgtattt atccggttct ctgaagattc ttactaacat atcggcttct
     5941 ttaaaaaatg tcttaatgcc ctgtttgtac gcagatttta cctgttgctt cacttgaatg
     6001 accgtacgaa ttaacgcttt acgagtggca actactagtc ccccattccg tccagctttt
     6061 atttgtacaa ataatttacc ttcggccatc aattctttta atgcacggtc aagagaacgt
     6121 ttaggaattg attgtccgtt gaaagtaatc gctgcttgca aatctgtttt cttcagtctg
     6181 acttcaggtc tgtatctata acattgggtt tcaaggtaag ctaaaatatc ttctttccat
     6241 tcggaaaggt gagatttctt tcttttatga cgttccttct taaatttgta ccaagtattt
     6301 ctttgttgtg taaacatttt ttcctcagac agcgattcta agccccaatt tgacactaac
     6361 tcctgtacat aagtcttact tgcacctcta tacttcccag agtaagcact ttttacaatt
     6421 cttactcgtt ctctatcttt tagaggacgt tctaaagaac tgttaaaaag atccatctca
     6481 tttagacagt cttctaagtc aactcctgat gcataattgg ccaaagataa agtgaaaatc
     6541 acattattcc ggcccataat ccctttagcg cctacaattt ttggattact aattaattgt
     6601 cgataccaca tttcgttcac ttgtttatat ttatttttaa caagtgaaag tagcggtttt
     6661 ttatatgttt tcgctggctc atatttcatt gaccattgga ttaattgctg tatatcatgt
     6721 gttaaagctg gataataata gacaatgttg tcagttctag ggatacgtgc tatcccaaaa
     6781 tgattgcaac ctaaatcgac tgaaggcaat tcttcagcaa atgcttttct taagttctca
     6841 gatactcgtt tggccacttc gattgactga taattatttt tggacgaaat ataccaggca
     6901 ttttcaagaa taaagtatgc ttggaagccc cctggtgttt ccaaaataag ggttggcatt
     6961 aattccagat cgattgctgc atcgagaatc atttgagaat ctaatttttc gcctgttaag
     7021 cgatcaaaat cgattacaaa agtattgatt tgttgcaagt tgcgttcgtt gtgccctttt
     7081 acaattattc ttttttcatc tgcatatgca ccatagctgt aaacgttagg tgtccaatga
     7141 gtaaatttat cttcattttc agctaagcct tcttgagaag taagaacgat tccacgagat
     7201 tccgtcatta acttcttgga tctaaaccca aaaatagcac ctttttttcc ttcagcttta
     7261 tagccgattg gtttaatttt gctgtttaaa cacttgtatt cacgaatacc actttttaaa
     7321 ataactgaaa tcacgtcaga aactttcatc ttatcaccac ccattctata tttaaaaaaa
     7381 ggagaactgc aggcattcta cagctctcct ttaaacctta tttgcagcta aaatatagct
     7441 caaaatcttg atttttaagg tcccaaatga tataattaaa aagactaaga gagctgcaac
     7501 tctctgtaat ctaaaactat aatgggacga aagagaatca tgaaatgctg tgtacatttt
     7561 gattctgtaa tttagttgat cggcaaatca actaagttac cgtctctatt tttttgtcta
     7621 taattcaatt agtaaatgta tcttttttct attcattcaa attcttcttt agaatacatg
     7681 tcactacaac ttttgtaaag taaaaatttt ctctcaaata atcattctta atttattttt
     7741 gtatcctttt atctttgtat ccttttatct ttttatcttt ttatcttttt atcctttaat
     7801 tttattgttg tatcttttta tctttgtatc cttttatctt tttatctttg tatccttatt
     7861 tagaagtcta tttaataact tttcttataa aatgctatct gattaaataa gtatgtttta
     7921 agataattag aaaaaagata catttatcta tttacaatta attctatttg tattactatt
     7981 taaatataga aaaaagatac aaagatacat ttatctttta ttacattttg attacaagga
     8041 aggagtatat tgatggctaa tacaattata tttggacaac aaaagggcgg ggtaggaaaa
     8101 acaactgaca cttgcatgat aacgttggta gctgcgatgc taatgaaaaa gcgtactatt
     8161 ttaattgata ttgatttgca agcaaatgca acaaatttta tgacaaaaac atttggggta
     8221 gaaacggtac ctcagacttt gatgtctgct cttgaaaatg gcgatctacg tcctgctatc
     8281 gtaaacctta aggaaaactt agatttaatt cccgctggtt atgatatgcg tctatacact
     8341 gaattcttaa cagaaaattt tgataataca atagatagaa ctttttatct taaaaacctt
     8401 attgaagaaa ttaaagacga ttatgattat atttttattg acatcccacc ttcaaccgat
     8461 ttaaaggtag ataatgctat ggttgcatgt gattacttgg ttgtagtaca agaaacacaa
     8521 caattttctt ttgagggaag tcaacggcta atttttgatt atatacaaac cttagttgac
     8581 gattttggct cagacgttcc aattcaaatt gcaggcattt tacctgcatt gttacaacaa
     8641 aaacgcccaa tgcaccaaaa attagttgag gaaacaatag acacatttgg aagagacaat
     8701 gtttttaaca ctattattca aaatcatgca cgtttagagt ggtatccacg gattggattt
     8761 caatttcaag actatcatga taaaaaaatg attgctcttt ttgcagatat tttctgtgaa
     8821 ttagaagaac gcatcactct ttataaaagt aatggtgata ttcaagatta tttttatact
     8881 ccgagatata tattagacgg aaaattaact tccctgggaa aggagattag tctaaatgtc
     8941 tagtttgata aatcgtaaaa tcccacaagg ggcaacagca aataaagaag cagatacaaa
     9001 acgaaaagtt actattcaaa aaaaatcgag taaaaagata ttagcttcag aaagaaaaaa
     9061 catcaaagtg tccccctcta cttttgactt aattaaaacc atatcaacaa tgaaaagcta
     9121 caaaaattat gaatttattg ataaagctgt agaaagctat atacaaaata atttaacaga
     9181 acgtgaacaa cgtatcctca aaaatttaat ctcaaaataa ataaaaaagg ataaaaagat
     9241 acatgtatct ttttatcctt ttttatttat tgatttcttt ttatcaatgc agattttacc
     9301 atttttatca agtagaggcg taattcctcc tttaggagtt tctaaataat tcacaccagt
     9361 aacagtatca actacaattt tatagcctaa tagagttaaa gattcacttt ctttttcaac
     9421 aaaacgtttg tctttaaata catgttcctt acatttatgt ctgtctaatg gtacgttatc
     9481 tgattgttct atcaactcac tatttgaatt atccccggca accatcatgc ctaaattttt
     9541 atcataataa atatattctc cacctagtat taaggttaat ggcataaaaa tcttcctttc
     9601 tttaattttc ttaccgtcct ttttctcttt ttttaacggc tttcgttact tacctttttg
     9661 ttcattttct acgcacaatc aatcgattaa acagattgcc tcttattcca ataagtaaat
     9721 ttcaagtgca aatcatccta tttttaaata ggatctgttg tcaacttttt cttaattcta
     9781 tcaaaaaacg gaagattttg cttgcaaacc aattcccttt tactaaatta aaataggttt
     9841 ttatttagta tgaaacagaa accaacattg aagtaaatct tatttgttta ttctttaatt
     9901 attgtaattg ctacatattc tccttgtttc tgtttgtatc ggcgatggca tttaggacag
     9961 taaaagtaat ttttttgata aacacttttt gctaattccc cacagtcaca ctccacgtga
    10021 aaagcaatat ttcgattaaa actagttatt tcaatgcccc aacgatcata agattttcgc
    10081 ttcataaaaa ttttcctcct tgtaaatttt actaacgatt ataaataccg ttgccattct
    10141 ttcaactgtt gactaattaa cgcataaaat tcgacttctg ataaaatggt aatctcatga
    10201 cctaacgcaa tcaagtcccg cgcggtttgt tccttttttg tatagagaga atcatcaaac
    10261 aacgacttag aatactgacc acaaaccagg aaatttgttt ttgaagtaac acttgattga
    10321 caatggccgt ttaaagagtg aaccaaaccg atggcttggg ttcgtgtcat ttgctgcagc
    10381 gttccagtaa aaacgaccct ttgattcgtt aagtccattt tcaaactctc ccttcccgtc
    10441 taatagctgt tatatctatc aagaaaagtg gtgcaaggta tgttcatatt ctttcacccg
    10501 tcgataaaag gtatttcgtt tcatgcccaa ttgatccata gcatagactg cagttatctt
    10561 tcctgctttc cagtcagaat agatcgcagg aaattcagga ggtaaagcaa ttttctttct
    10621 gccgaatttt acaccctttt gtttcgcagc ttctacgcct tctctttgcc ggctattaat
    10681 tcgtttccgc tcgtcttcgg ccatatagct gagtaattgc agaattaagt cagagataag
    10741 cgtctctaac ccatctagtt gcttgtaact agcagtatct aacagaggca tgtctaacac
    10801 agaaatatca atctgctttt ccttagttaa ttctcgccat tcttctaaaa tcatttgttt
    10861 atttctgcct agtctatcta atgattgtag atagagtaaa tctccttttc gaaacatccg
    10921 tttcattaat tgatattcag ggcgtttaaa atctttgccg ctggcttttt ctacaaatat
    10981 atattcttca gggattccta attccttcgc ggaaatcagt tgtcgttcga tcgattgatc
    11041 ataactactg acccggatat aaaaaaattt tctcatcgtt tttatctacc cttttttcac
    11101 taaaattgtt tcaaaacaat cagattaaat cagaaacgtt tcaaattgta ttatgtggtt
    11161 attatgccat acataaacaa agaaaatcaa ggcatgtcaa aaagtatact ttttgaaaca
    11221 gtaagtttac actcccgcat gattatattt attccgctaa aatacataaa aatggcaggc
    11281 cttaacggtc tgccattaaa taaactagtt atccagtttt tcttttatgt cttccatggt
    11341 atattttatt tttcacatca gaatttgact tctttaactt ttacaatttt tttaacacta
    11401 ttattctatt acaacactat atatttctaa tttagggtta aaaataaatc tttttgatac
    11461 ttttgccttt acaatataat tgtattcttt ctttttttct ttgaagtcgc ttgattcaac
    11521 tgtaacttca aaccacattt catttggaga ctcttttaat ttttttactt gaaatgaatt
    11581 atctcttgga tcataaggtg gtactctttc aaactcagga agttttctct gtatttgttc
    11641 ttcaggagta ttcgcaaagt aatctactat cttgacagtt attaaaagta ctactattcc
    11701 caaaactata acaaacataa agataattcc acatccagaa ttatttttat tattttgagt
    11761 cagattattc cattctattt cttccataat catcatgtcc ttttacaatt catatactta
    11821 ttttatcgta tatatttaaa aaggaaaagg acactgttta aatagtaaac gattcttaat
    11881 taagtttact tctattttct tgaaattgaa taattttttc agaaaatcca tttgcaactt
    11941 tcccctcatc tccaataaaa tcaagataag taaaatctgt cccaagtgaa ttattaacta
    12001 ttttatcaat gaaaaatgaa attatcccta cacctaagat atcaactgtt tcgtcatttt
    12061 ctaataaact atttgttact gaagcgattt tatctgaaga accaaatctg ttcttaacaa
    12121 attgattaaa tctatcaata gcacccttat cacaatagta cgtttttaaa gccgataaag
    12181 ctgttgaatc agaatttctt tttgctaagg tataaacatt taccgcgtca acatcttggt
    12241 ataaatcttc tagactaaat gttccacctg cagccgtacc tactaatgca aatacacctt
    12301 gctccatttg tattcctaat ttcttaactt caccccagca agtcattaaa tctcctaacc
    12361 aacctgcaaa gtcagaaaca ctatcattga ttgtgtcggc tagaaacatc tgtgattgta
    12421 acgtcacaat taagtgaggc aaacctatta ttgaaccagt attaggatct ataaaataat
    12481 tatacaggcc ctcgctatta gttaatttat tcctatattc atcataaatt tttctatcat
    12541 gctctgttac aggagaagaa atgatgtccc atgacaaacc agaataattt acatatctat
    12601 aaaatgtcaa atgagggttc caaggattat caagaccagc ttgtgataga tattgatacg
    12661 cacgtaccat ctgatttata accttctgat tgtatttggc aatgtaatta tcattattag
    12721 tatcatttga taactcatta aatccgctat ctctacccga tgtagcgatt aaatcgatac
    12781 caacagctcc gttgccactt cctatggtaa attcaacaaa ttgatcaaaa gaccattgtc
    12841 taggcatagg aaaacctaaa ttcccactat aaccagtcga catatctgaa acaaatgcaa
    12901 acgaagcata cccgttattg attatttgag aacaaacatt tcttgttccg taaacacccg
    12961 tttgataccc gctatatgac attacagcag ataccccctt aaaatattct ccagcatatg
    13021 caagaatttc ttcccctaac atgtctagat caacagcaaa ataaataact gttgttaatg
    13081 gtatatttag attttttgct gcatctatag ccttcttacc atctgataag ccacgatcat
    13141 aagtaaaata agctaatgct gcacctccat cttgatagat aggaaacaca gataaatttt
    13201 tagaaaaaat attattcaat tcctctgtag ttagatactt atttcgttca tcttttccaa
    13261 ttccaacagt accagtaaga tatctaccaa ctatttttat atcattatca accaatgttt
    13321 ggatttgaga tctagttaat tgcgtagaag tatctgcgcc atctgcagat cttctaatat
    13381 ctccagcgct agacaatagt ccttttatta caacaacatc tgctatttca gtatattcag
    13441 gcaataccat aaattttcgg aatgccagta cttctaaacc catatattta gtgaaaatac
    13501 catcgaatgt accgtcttca aaataaccat taacatataa tccatattga attattctta
    13561 catagtttcc cgagttacct tcagacacag gataagtttt actagttgtc ccattaccat
    13621 aagaccctgt tgcttcactg ggagaatatc ctagttcagc ttgtaatgca aaaattaatg
    13681 cttgatttga atttctttga taaataccat cacaaggcat tattcccgtg tattctaaat
    13741 aatttacatt taaccattgc tgcatttttc taattttact atctccgccg ggaacaagaa
    13801 caaaagcact catatcaaat aacgctttgg cccatttcgc atccaaaact ccagttacag
    13861 cgcttccatt aaaccctgca tcagtctgca actcttttac agcagtatct aaatatggag
    13921 tatatacccc atcgaagcca cctggattaa tccccttaca ccaaaaggca ccttgaataa
    13981 gaaatacaat atttttaggt agatttttgc cccatttagg agtaacctga ttatcgtaca
    14041 ttttttcagt ggtaggtcca aaattagcta ctagattagt aataccaagt tctacttgta
    14101 gtccttcaat aagtccatag attgtaggcc accctgttcg cccattttct ggaaccttat
    14161 caaaccctgt aacattactg tatttcttgt ttagccattt ctgagtactt aaaaccatct
    14221 catccataaa aatcccctgc tttcttgccg aacatacgtt cgtttatgtg ttgatt
//
LOCUS       DACCXW010000071        14228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCXW010000071 DACCXW010000000
VERSION     DACCXW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.072"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            39..272
                     /locus_tag="IVY71_001808"
     CDS             39..272
                     /locus_tag="IVY71_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7985861.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(371..802)
                     /locus_tag="IVY71_001809"
     CDS             complement(371..802)
                     /locus_tag="IVY71_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7985862.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            881..1294
                     /locus_tag="IVY71_001810"
     CDS             881..1294
                     /locus_tag="IVY71_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7985863.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(1332..2480)
                     /locus_tag="IVY71_001811"
     CDS             complement(1332..2480)
                     /locus_tag="IVY71_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7985864.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(2602..3198)
                     /locus_tag="IVY71_001812"
     CDS             complement(2602..3198)
                     /locus_tag="IVY71_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7985865.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(3398..6040)
                     /gene="alaS"
                     /locus_tag="IVY71_001813"
     CDS             complement(3398..6040)
                     /gene="alaS"
                     /locus_tag="IVY71_001813"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7985866.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            6428..6859
                     /locus_tag="IVY71_001814"
     CDS             6428..6859
                     /locus_tag="IVY71_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7985867.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(6904..8253)
                     /locus_tag="IVY71_001815"
     CDS             complement(6904..8253)
                     /locus_tag="IVY71_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7985868.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(8323..9486)
                     /locus_tag="IVY71_001816"
     CDS             complement(8323..9486)
                     /locus_tag="IVY71_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985869.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            9615..9938
                     /locus_tag="IVY71_001817"
     CDS             9615..9938
                     /locus_tag="IVY71_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985870.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(10121..11470)
                     /locus_tag="IVY71_001818"
     CDS             complement(10121..11470)
                     /locus_tag="IVY71_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7985871.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(11539..12492)
                     /locus_tag="IVY71_001819"
     CDS             complement(11539..12492)
                     /locus_tag="IVY71_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7985872.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(12594..13784)
                     /locus_tag="IVY71_001820"
     CDS             complement(12594..13784)
                     /locus_tag="IVY71_001820"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7985873.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         4177 a         2951 c         2460 g         4640 t
ORIGIN      
        1 gaaggctatg atcagacttt agaaaggtcg tgagtgcttt gaccacagga attgtaaaat
       61 ggtttgacaa caaaaaagga tacggcttta tcagttatga tgacacagaa gaaatttttg
      121 tacattttac tgcgattgaa gaagagggat tcaaaacttt ggaagaaaat caagtggtcg
      181 aatttgagat catcgaaggg aatcgaggaa cacaagctgc ccatgtcaag aaaggcaacg
      241 ttcagactac cgatactagt atgcatgaat agataaacaa atagagtgag acgctaaacg
      301 ggatgaaccg tacgtctcac tcttttcttt tcctgtaaaa attcgttttt ttaggttctg
      361 atttttaaaa ttagcttttt ctttttttcg tataaggata aggaattgat tctgatttgg
      421 gtccgttttt ttcaaaatga tcaaaaagga ctacttgatc tttttttcgc atcatgaaaa
      481 cagatggggc tttctcatct tgcaacatca tttgcacccc agtattagtt ggcgtcaaag
      541 atcgaatctg tccgacagat atttctcggt ctttccaaaa agcagcataa cgtataatca
      601 cagagtttga ttttaaaata aaagaacgcc gcaaacctag aaatagaaaa acaagaaaaa
      661 tacctaaaac aaaattgctt ttccaataag gacctgtgtt ttctaaagaa agaatcagac
      721 taaaaaacag aatgatcaag gtcaatgacc aatagatgat cgtatgtgcc agttcaggct
      781 gccatcgaat attttttttc attctctaac ctctcaccga ttcagtaatg atataatctt
      841 accataacat tagtttgaac aaaaaggaga atctcttttt atgttaaaag tatacattga
      901 tgcctctaca aaaggcaacc ctggtcctag tggaggtgga atcttgattg tctatcaagg
      961 taaacaagaa cagctatcta tcccgctttc tatagggaca aaccatcaag cagagttcga
     1021 agtcttttta aaaactttag aatttctaaa gaaaaacgac ttacagaatg aaacgatttt
     1081 ctgtttttca gacagcaaaa cactagtttc tacggtcgac aaaaacgcaa cgaagaacga
     1141 gctcttcctt ccctaccttc aaagaatcca agaacttcta tccgaatttt ctttattgat
     1201 cctgcaatgg ttgcccgaaa acaaaaataa aggagcagat aatcttgcac gtcaggctct
     1261 ccataaacaa ttaaaagaaa aaaagactct ttaaattaaa aactttaagg agtcttttta
     1321 tagtttctca tttaatataa aagataattg tgatcaaacg taatttccaa ttggtaatct
     1381 ccgatttttt cttctatttg actacgtaag attctttcta gttctgtatc aacaaactgg
     1441 aatttctcag ggaccactac atcaaattgc agcgtttttc cttgatgaat tactctaaaa
     1501 tcatgaatat taagccctgt acctgtttca agaagaattt gctttaactg ccgcaagata
     1561 ttatagtatt tttcgttatc aacaggcatt ggatccaagt gacagacaag ttccacatcc
     1621 aaagactttt taaagtcttt ttctatcaga tcaataatct tatgagccac attcaaattc
     1681 attgattcat ctacttcgat gtggactgat gcaaagcgtt tattgggtcc atagttatgc
     1741 accaataaat catgatagcc aagtatatca tcgcaagcat ttaaatgctc ttccatttct
     1801 tcaatctctg ctggcgttgg gcgacttcct aatagttcgt atacaaaatt tcgaagcatc
     1861 gtcaatccac tataaaggat atataatgct aaagcaaaac ctatatatcc atcaatacgc
     1921 catccagtta cccattcgat tgctgctgaa agcaatacag ccagtgtagt aaagacatca
     1981 ttataactat ctgttgctgt agctctcaat gtttcagaat caatggattg cgcaataacc
     2041 tgatacattt tgccttgtgc aaatttcaaa aagatggaaa tgattaggac aaaaaagacg
     2101 atgggtgtta aagagacatt ttctggatgg atgatttttt cagcagaaga ctctaaaaat
     2161 tgaaatccta cataagtaat gatgatcgaa acaaataacc cactgatata ttcgaagcgt
     2221 tcgtgtccat aaggatgttc ctgatctgct ggctttgcag caattttaaa tccgatcaaa
     2281 gtcaatatgg atgaagcagt atctgataga ctgttcactg catcagccat aatggagaca
     2341 cttcctgaaa aaagcccgat cattagctta gagacaaaca agatcagatt cgtaaccaat
     2401 cctaagatac ccgctagtac tccttttttt gtccgctgtt gcggtatttg tttctttttt
     2461 tcttcttgtt tcttagccaa gttttttacc ccttctagtt tgcttgttgc tcaggtaaaa
     2521 agatggagag gacgattgtt ttcctctcct ctcttccttt ttctatctat cgtgcgtcct
     2581 attactatta aaagatggtc attattctgg tttcaacggt gttgctgcaa ataacgcttt
     2641 tgctagtaat ggtgttatga ttcccgcacc tattgcttct ggtatcccat tgattgctgc
     2701 aacccctgct aaggttttaa ataaaagagc agagtctaca ccataggcat ctgccgttgc
     2761 acctgtataa aataatccca tgaatcctaa cactaagact gtattgacca atgaacccag
     2821 tacccctgca aaggctgagc tgatcgttat tgatttattt ttttgataaa tccagtggaa
     2881 aaccaagcct gctactaagc caacaagtac tctgggaact accgagataa tcggattcgt
     2941 gaacactaat gtatcaaatg gcgttgttgg catggcatag gcacgaacaa ttgtagcgat
     3001 tccccaaaac aagccaatca ccatgccatc ttttgggcct aatactactg cagcaataat
     3061 tactgtgata tggataattg ttaaactaat gaatcctaat ggaataaacc ccagccaagg
     3121 aaccatcgct tgtacgatga tgattgctaa caagatcgca cgtaagacta gtcgaaaggt
     3181 cttatttcta ctattcattt tttcacccct ttttatttat actaatcgta aaaatttctg
     3241 tcactttcgt caactaaaga ccatccgaat ccgaccattt gattagatgc gccatccaat
     3301 tgaaaaaagt atgagggcaa gacttgtttg tcttcgcccc cataatcatg tttcgttcaa
     3361 tgtttgaact tttgtttttc tgtcaaacgt aaacgcccta ttgttgcgca acccattctt
     3421 tcgcagcttt aagtgcatct ggaataccag caggattttt cccgccagct tgtgccatgt
     3481 ctggacgtcc accaccgcca ccgcctactt tcggtgcgat tgctttaatc aagtcgccag
     3541 cttttagtcc agcttcattt gattctttcg tcattgccgc tagcagactg acttttcctt
     3601 cttgttcgtt tgctaaaaca agaatatctg aaatattttt ttgtttccat tggtcagcta
     3661 attgacgcag ctgattcata tctttcactt ttacttgtgc tgcaatgatt gtttttccat
     3721 tgatcgtttc tggatctttg aatacatcac cagcttgttg attcgctaac ttgctagcta
     3781 attgttcatt ttccttttgt agctctctta cttgctgttg caattgttcc gcttttgaaa
     3841 caacttcttt caattgaggt gatttaacaa ttgttgctac ttgttttagt tgtttttctt
     3901 cttcttcaag tagttcgtac gcttctttac ttgttacagc ctcgattcgt cgcacgcctg
     3961 caccaattcc tgattcagaa acgattttga agattccgat gttttctgta tttgctacat
     4021 gtgtgcctcc gcacagttcg attgaccagc cgccaatatt tactactcga acgtcatgtc
     4081 cgtatttttc accaaacaaa gccattgcac ccatgttttt ggctgtatcg atatctgttt
     4141 caattgtttc aactgggata gcttcccaga ttttttcgtt gacgattctt tccatttcag
     4201 ctaattcatc tgaagtgatt tgaccaaagt gggtaaagtc gaatcgtaaa tgtccaggag
     4261 cgactaaaga accagcttga tttgcatgct cgccaagtac ttctttcaat gccttgtgca
     4321 gtagatgggt tgctgtatgg tttttgatga tccgattgcg catcgattga tcgatcaata
     4381 gatgataggt ttgtccttca ctcattggtg cttttacttc tacggtgtgc ataaattgac
     4441 cattaggcgc tttttgaaca tctgtaacaa ctgctacgat ttcgcctact tcattcatga
     4501 tccatccttg atccgctact tgcccaccca tttctgcata gaaaggtgtt tgatcaaaga
     4561 tcatttgagc ttcaccttct gcgacttcat tcaaaagaga ctcaccttta acgatgacca
     4621 ataattgact atctgattct tgttctgtgt atccaatgaa tttactttct actttgatat
     4681 ctgtcaataa agcagattgt acccccattg atttttcagt actgcgggca gagcgagcac
     4741 gttcgcgttg tgcatccatt tctttttcaa aaccagcatg atcaactttc agtccttcat
     4801 cttctgccac ctcttccgtt aattctaccg ggaagccata tgtgtcgtat agtttgaaga
     4861 tgtcttttcc tgctagagtc gtcccgtttt cgttttttac ttcttggatc acttgattta
     4921 agatttccaa accttcattg attgtttcat tgaaacgttc ttcttctgta cggataactt
     4981 tttcaataaa ttctttttgt tgaagtactt caggataata acttaccatg atttccccaa
     5041 caacagggac taaacgatat aagaatgctt gttcaatacc taattttttc ccatgcatga
     5101 cagcacgacg aagcaaacgg cgtaagacat accctctccc ttcattcgat ggcaatgcac
     5161 cgtcgccaat agcaaatgaa agggcgcgaa tatgatctgc gattactttg taagaaatat
     5221 ctgttactgg gttttcacca tattttactt gtttgcttag cttttctacg gcgtggataa
     5281 taggcataaa caagtctgtc tcaaagttcg ttgacgcatc ttgaacgatt gagaccatgc
     5341 gttccaatcc catccccgta tcaatgtttt tatgaggaag tggttcaaat gtgttatcag
     5401 gcttatggtt gaattctgaa aatactaagt tccagatttc taaataacgt tcattttctc
     5461 ctcctggata gttttcagga tcgtcttctg ctaaatcgtt ataagcttct ccacggtcat
     5521 agaaaatttc cgtatccgga ccacatgggc ctgcaccgat atcccagaag ttgtcttcta
     5581 catcaataat gtgatctagt ggcaggccaa cttcttcatg ccagatgcgt tttgcttctg
     5641 tatcttttgg ataaactgtt acataaagtt tttctggatc gaatgccatc cattttggat
     5701 cagtcaaaaa ttcccatgcc cagtggatgg cttcattttt gaaatagtca ccaatcgaaa
     5761 aattacccag catctcaaac atcgtatggt gccgtgctgt tttaccgaca ttttcgatat
     5821 catttgttcg aatagatttt tgggcatttg tgattcttgg attttctggc acaactgaac
     5881 catcaaaata ttttttcaat gttgcaacac cagagttgat ccacaataag gttggatcgt
     5941 tcacggggat caaagacgca ctaggttcaa tcgtatgtcc tttagattgg aagaaatcaa
     6001 gaaacatttg tcgaacttgt gcactactta attgtttcat gtcattacct gctttctata
     6061 aattaaatac aaaaaaacac catcacagcc aaggacgaaa actcgcggta ccaccttgat
     6121 tgcaatgatg tgtaatcatt acctcttaaa ctcgataacg ctgagctgcg tcggtatttc
     6181 taccaaaata gaaaaaggga gcaatttgat tagtgactat tcttttttca gccaagaaag
     6241 aattttctgt gagtcttaaa aaacaaatca tatccttttc gtacttaatt agtatacgca
     6301 ttctattgat cactgtcaat atctcaattt ttattttcat ttccttttgg tcatactcca
     6361 cgatttttgg tacgataaga ataaaggaat caatacatct ctgctattca tgacaaggag
     6421 gaatatgatg aaaaaaatag ttggagcgga tgaatggata cgcgcagcta gctgcttttt
     6481 gagatatcaa gtttttgtat tagaacaagg gattcctgca gaactagaat tcgacggaaa
     6541 agatatcccg caacaaacct attatctatt tcttgatcaa aaaagaccag tagctacttt
     6601 gcgttatgaa aagaaaacac ctacttgctt caagccggaa cgattttgcg tagcaaaaga
     6661 ataccgagga aaagggttag gcggtcagtt gcttgaagct gctgaaaaac gggcggtttc
     6721 agaaggttgt cagactagtt atgtagtagc tgaaatgacg gctgtagatt tttatgaaaa
     6781 acaagggtat catacttgca cagaaccttt tattgaagat gaagtacttt gtatcggaat
     6841 gaaaaaaagt ctagcttaaa aacaagagat tgtgacacgc cttttgtcac aacctcttgt
     6901 tttttattta tgtgaatttt tcttattttt acgagcagta cggctttgct gtctgcgttc
     6961 gattttgcgt tcttttttat ttttttctgc aattgcccaa tcgatttttt tcttataacc
     7021 aggcttgatc tttttctttt tcttcttcac aagaccaatc agcgtgggat ctaatacatc
     7081 ttttgatttt tctcgtttgg cacgtctatt acgatcgtat gttttgacga tttccccgtt
     7141 tttgatttct tttggttcaa atgagacgcc caatttttct atcgcatcaa tggcttgctc
     7201 atcatttggc gaatataacg taatggccgt tccttttaag ccattacgtc cagtacgtcc
     7261 gacacgatgg ataaagaagt ctagatcttc tggaatttct gcattaataa catgagagac
     7321 accttcgata tcgatccctc tagcagctaa atcagtcgct acaacatatt gatactccag
     7381 attctgtact tgtcgcatca ctcgttttct ctctcttggg gtgatgtctc catgaatctt
     7441 tgctactttc aagccttggt ttttcaagta atcggcaatt tcatccacat gctgttttgt
     7501 attggcaaat acgattgcta aataaggatg accaattgtc agcagttgat aaatcatttg
     7561 attctcatct ttaccttttg ttgaaatcag ccaattttca atgtcttcag aaataactgc
     7621 ttttggtttg atttcttcaa tgattggatt ttccatgtat tttcgtaaga atggacgaag
     7681 tttttctgga atagtggcag aaaaaactaa aaactgcagc ttttctggta atcgacttgc
     7741 aatttggtct acttcattta aaaaacccat atccaatgtc atatccgctt catcgacaac
     7801 aaatgcaaat gcggtgtgaa tcttcagcgc ttgttcattc atcatgtcta atattctacc
     7861 aggcgtgcct actacaacct gtggctgttg gtgatgcaga cgatttaatt gacgctgttt
     7921 gtctgtaccg ccgacaaaat tcgagacacg gatctccggt gtagaaaatt tagcgatttg
     7981 tgtagctgct tcatagatct gtgcagctag ttccctactt ggagctgtaa taacaacttg
     8041 tacttcctct ttttctgcat tgattttttc aaacaaaggc aacaaaaacg tatgtgtttt
     8101 tcctgtacca gtttgagatt gcccaatgat attttttcct tttgcaatta aaggaatcaa
     8161 acgttcctgg acctctgtgg gctgtttgaa gccctttgcg gttaacgctt cattgataaa
     8221 cggtttgaag cggaatatat caaaattatt cattcgtgca cctcttctct ttaaattatc
     8281 acgactttgg cattataaca cagacaaagc gtttttgctc agctaatttc gagaatcatc
     8341 atcctttgtt tggccaatcg tgctaacagt atatgtgtga ctagcttttt cataaaacag
     8401 aatccgtttt tttctcaaaa atgatagcaa gatatttaaa ataaaaatac cttgtagtaa
     8461 caagacaccg agagaaatga atactgcact catctgcaac accgtattcg ttgtcgaatt
     8521 catgatttgc tgaagcaact gccccaacca gcttaaacca taaaaaagaa agtacagata
     8581 cccgtagcgg atcatcaatg cttttaaaga aattcgttcg cggtcttctt ccaccaaacg
     8641 gatacgcaca atttttttgc caagtgtcca tccattggtt ataaacggta caatcataaa
     8701 gtatacccct acttctaata aaagccacca gatatgagaa gtaactgccc ctgaatcgaa
     8761 cgcaatttta aagaaaatcg ccgctaccaa attaataaga gataaaaatg tccagtcgat
     8821 caaataagcg accaagcgtc gagtcaatga gacagtcttt cctttttcat aagaaagttc
     8881 atccattcgt cttcttgtag gaagcataaa tgtaaataac ggcgtaacga caaaaccaat
     8941 gattccaccc aatgtatttg tcactaaatc attcacatca aataagcggt atggtcttgg
     9001 gtagataaaa taaagtccac ttagctgcgt caattcaaaa aatagagaaa ggcagaaact
     9061 agcaatcatc gttttcataa aagaaagccg gaaataatat cttaaataaa tgccaaacgg
     9121 tatgagtaat aaaacattaa aagctggttc caaaaataca ctctgcttca ttgccggcag
     9181 gtaggtacta ggttcagaca agaccaatac cgtgttgtct ctgaaatgtt gccatgaagc
     9241 aaataaatgc agctccatct ttggccctgt catttgggca accgtctcct ttgccggaag
     9301 tggtaagatc accaaaaaat acgcacacaa taaataaaaa acgaaagaat acaaaataac
     9361 tgcccttgtt actagaaatg ttccatactt tcgatattca taaataaaaa aaacagatga
     9421 gatcgctaag gctaaaaatg gaaaaacaat cagcgcaaaa cgaatcggtt cgatataact
     9481 tgccatatga ctcctccttg attctttgta tctctcttat cttagcagtc ctttgctgtt
     9541 tcaagcaagt aacgaggcta ttatttctca tttctttcaa agtaaagtaa gataaacaca
     9601 aagggggtgt cgagatgctg aatatgtact ttgtttttgg cgtacctatt tttttactgt
     9661 tcctttatgc tacgatcgct tatgtaagaa aaagaactac aattcattat ttaggattta
     9721 ttctcctgat tatttcgggt ttcatgctag tcttcaatct tcagacttgg cagcaagcac
     9781 ttcttgaaat ggataaaatg actccacatg ctttgtctaa agtgcttggc taccctgtct
     9841 atctgatttg gcttcccatt ttcatttctg gttgcttggt tttattaaat atttatcgcg
     9901 gcgtacgccg aatagttcag ttacgcaaat caaagtaaaa gacagaaatc ggttgagccc
     9961 gatttctgtc ttttactttg ctatttcatc gtagacaaaa cgacatttac gtgatcaaaa
    10021 ttaacgagta cttttgacat ttcttccacg tactcctcat ttaaataaaa tattttgttt
    10081 ggcagagaag tgcagttcta ataaaattcg aatgttttag ctatctacgt tttaaataga
    10141 gataaatcgg gataccagca agtgtcaata ctatccccat cgaagctaaa aaggtctgag
    10201 tgataagcgt gcttgatacg ataaaagtcc ctccaatgat tgctactagt ggaataaacg
    10261 gatacattgg aactttgtat ggtcgaaaca aatcaggttc ttttttacga agaagaatga
    10321 caccaacaaa gaccatcgta taaaaaatcc aaatgacaaa gatcagcata tccgttagtg
    10381 tatcaaaacc accgatcatc atcatgccaa tagcgatgac tagctctaag atacctgcgg
    10441 ctacaggaac tttagtttta tcatgaagtt tagcaaagag cttgctaaaa ggcaggttgt
    10501 tttcttttgc cataacataa gggagacgca ttccagtaag ggtatagcca ttgatggttc
    10561 cataaacaga aatcaagata ccaactgtga ccagtcttcc accgaagccg ccaaaaatca
    10621 tttttgcaac gtcacttgcc gcattgctgt ttcctgcgac tccgtcgata gatgcggttc
    10681 ttaagaacac cgcatttact aataaataaa cgatcatgat tccgatgatt cctaaagaaa
    10741 ttgcttttgg caaatctttc gccggctttt tcaattctcc cgaaatattt cctacgtgta
    10801 tccaaccgtc gtatgcgaac atcgttgcta gcaagcccgc tcccaaggct gagaaaaatg
    10861 aaagattttc tcctgcttgg atcggaaaca gttggaagtc tactccttct tgtcggaaca
    10921 aaccgaaaat gacgatcaca aacaaaggaa taagcttgca gacaagcgta atcgactgaa
    10981 aagcccctcc agctttagaa cctaaaaaat tgatcaacag gatcgataca gcagctgtaa
    11041 cagctacggg aacaatcatt gactgggaaa gaccaaatag attgacgaac tgtgtaccga
    11101 aaatgatcga caaggctgct acatttgctg gaaaataaat cacgacttgc gcccagccca
    11161 ataaaaaagc tgctgtattt ccgtaaatcc gttcgatata tttaatcatc cctcctgttt
    11221 ctggaatagc agctgccagt tcagctcctg ttagtcctgc acagacagaa atcataccac
    11281 cgagaaacca agaaaacata tgcaaactgg cggatcctgt aacttctgct acgcttgcgg
    11341 ctttaaaaaa tacccctgca ccgatcactg tgcccataac agtcgataaa gcagtaaaaa
    11401 atcccatcgt acgttttaat tcattttcta tttttacttc ttttactggc gtggcttgat
    11461 attcttccat cccttgtccc ctttcatacc atagccaatc ccaaaagtat aaaaaacttt
    11521 ttggatgtgg ttcttgtttt ataccgtctc taccaatttt tctttcagtt tttccaacat
    11581 atcctttgtc atcacatcta agtcgtattt cggcgaaaag ccccattcct cttgcgcaca
    11641 agaaacatct agtgaatttg gccaagattc tgctattgac tgacgtacag gatccacatc
    11701 ataatccatt tcaaaatctg gtatgatttt ttgaatagat gctttgatct cttccgggtc
    11761 aaaagacatc gccgatatat tgaaggcatt tcgatgaacc aatcgatcag gatctgcatt
    11821 catgagtttt ataatggcat cgatcgcatc tggcatatac atcatatcca ttgatgtacc
    11881 ttgatcaatg aaactagtgt actttccttc tttgattgct gaataataaa tatcgactgc
    11941 gtagtctgtc gtaccgcctc ctggcaaggt tgcataagaa atcaatccag ggaaacggac
    12001 accacgagta tctacgccat atttcacata ataataatca catagtagtt ctccagctac
    12061 cttcgttacc ccatacatgg ttgtaggacg ctgaagagta tcttgtgggg tatgatcttt
    12121 tggagtattt ggaccaaacg cgccgattga acttggcgta aaaaatttca gctggaattc
    12181 tctagcgact tccaatgcat tgaccagccc agtcacgttc aaattccagg ctaattgtgg
    12241 ttttgcttct gcaacagctg acaaaagagc agccaaatga atcaatgtgt ccacttgata
    12301 ttgttctgct atgctgcgca ttttttcgta atccattaca tctaggatct caaagatatc
    12361 gtttttctca atgacttcag gcatgcgtat atctgtcgca atgacatttt cactgcctag
    12421 ttcttgacgt agccgtgtga tcaactctga accaatttgt cctaaacatc ctgtcaccaa
    12481 tacttttttc atgatgatcc tccctgatct tttttactac tatcttcaga tgattaacaa
    12541 tatatgcgtg aacatgttct ctaattttaa atagacgaaa aaactaaatt tagttaaata
    12601 atttccattt cttttcctat tttttgataa actgctactg cttcatcaag catttccctc
    12661 gtatgttccg cagtcggcat atttctgatc ctgcctgtcc caagaggcac agttggataa
    12721 acgattggtt tagcataaat cccatgttcg atcagcttct tagagaaagt ctgagtcact
    12781 ttttcgtctc ccaaaatgac tggagtaata ggtgtttcag acataccgat gttatagcct
    12841 acttttttca attcttcttt aaaataatta gcattttccc aaactttttc gacaagttcc
    12901 ggatgttctt gcatcaaagt aatcgaagcc aatgcagctg cagctgcacc tggagttagc
    12961 gaagtagaaa ataggaatgg ccgacttcta gctttcaacc agtcgattag tgttttcgat
    13021 ccagctacat aacccccaac aacacctata gctttcgata acgtgcccat ttgcatatcg
    13081 attttgtcgc ttaatccaaa atgcttgacg gttcctgcac cttttccagt tacgcctgag
    13141 ccgtgcgcat catcgacgta ggtgatcagt ccatattttt caacaatcgg aataatttcc
    13201 ggcaatcgtg caatgtctcc atccatggag aaaacaccat ctgtgatata catgattttt
    13261 ttatatttct tactttctat cgcttccttt gcttttttct caaggtcttt catatcttga
    13321 tgtttaattc ggatgatttt tgctccggat aagcggcaac cgtcaataat agatgcatgg
    13381 tttaattcat ccgataagat agcatcttct tttgtcatta ctgcagagat agcgcccata
    13441 ttgcagttga atccagattg aaaggcgatt gctgcttctg tccctttgaa ttctgcgatt
    13501 ttttcttcta attgctgatg gattttcagc gaaccattga tcgttcggac tgctcctgct
    13561 cctacaccat aagtttcggt tgcttcaata catgcttttt tcaattcttc gcgattggca
    13621 aatccaagat aattgttgga tgctagattg atcatctttt ttccgtctac gatgatacaa
    13681 gcaccatttt cactttctag tatgtcgatc gtattgtata accccttttc tttcagttga
    13741 tccaattctt tctctaaata cattgttagt tctttggaag acattgtctc cacctccata
    13801 ttcatctgtc ttgcagaaca taccaaacat atatcgtgtc attcatctac aaagcaagta
    13861 tgctatattt ttgataacgc ttacactgat tatgagaatt atttaaccac attttttatt
    13921 tacagtatct ttttactttc aaatgactta tatagtatga acaattacct tttagataat
    13981 gatctatttt actctacctg ggcagataaa tataacacaa ccattgatag aagccctttc
    14041 gttaaaatca attgtattct gaatatttaa tttatttatt gcatagacca ataaaaatac
    14101 ataaaatttc catcttcgtt tatcagcaat ttttaatttt actctctgaa tatttcttaa
    14161 aagttttact tgttgaattc gaaaaaagga ctgtgacaaa agtcttgtca cagtcctatt
    14221 cttccgaa
//
LOCUS       DACCXW010000072        14181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCXW010000072 DACCXW010000000
VERSION     DACCXW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.073"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..475
                     /locus_tag="IVY71_001821"
     CDS             <1..475
                     /locus_tag="IVY71_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7985874.1"
                     /translation="LEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALN
                     QWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILR
                     RNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            981..1583
                     /locus_tag="IVY71_001822"
     CDS             981..1583
                     /locus_tag="IVY71_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7985875.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1599..2276
                     /locus_tag="IVY71_001823"
                     /pseudo
     CDS             1599..2276
                     /locus_tag="IVY71_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2788..3084
                     /locus_tag="IVY71_001824"
     CDS             2788..3084
                     /locus_tag="IVY71_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP7985876.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            3085..3501
                     /locus_tag="IVY71_001825"
     CDS             3085..3501
                     /locus_tag="IVY71_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7985877.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3521..5065
                     /locus_tag="IVY71_001826"
     CDS             3521..5065
                     /locus_tag="IVY71_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7985878.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5208..5432
                     /locus_tag="IVY71_001827"
     CDS             5208..5432
                     /locus_tag="IVY71_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7985879.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5447..6316
                     /locus_tag="IVY71_001828"
     CDS             5447..6316
                     /locus_tag="IVY71_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7985880.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6297..7031
                     /locus_tag="IVY71_001829"
     CDS             6297..7031
                     /locus_tag="IVY71_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7985881.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7064..7972
                     /locus_tag="IVY71_001830"
     CDS             7064..7972
                     /locus_tag="IVY71_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7985882.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7969..8511
                     /gene="sat4"
                     /locus_tag="IVY71_001831"
     CDS             7969..8511
                     /gene="sat4"
                     /locus_tag="IVY71_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP7985883.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8604..9398
                     /locus_tag="IVY71_001832"
                     /note="aph(3')-IIIa"
     CDS             8604..9398
                     /locus_tag="IVY71_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7985884.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9506..9694)
                     /locus_tag="IVY71_001833"
                     /pseudo
     CDS             complement(9506..9694)
                     /locus_tag="IVY71_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9674..10246
                     /locus_tag="IVY71_001834"
     CDS             9674..10246
                     /locus_tag="IVY71_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7985885.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            10586..11440
                     /locus_tag="IVY71_001835"
     CDS             10586..11440
                     /locus_tag="IVY71_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7985886.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            11539..11748
                     /locus_tag="IVY71_001836"
     CDS             11539..11748
                     /locus_tag="IVY71_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7985887.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11765..12037
                     /locus_tag="IVY71_001837"
     CDS             11765..12037
                     /locus_tag="IVY71_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7985888.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12039..12902
                     /locus_tag="IVY71_001838"
     CDS             12039..12902
                     /locus_tag="IVY71_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7985889.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13165..13902)
                     /gene="erm(B)"
                     /locus_tag="IVY71_001839"
     CDS             complement(13165..13902)
                     /gene="erm(B)"
                     /locus_tag="IVY71_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7985890.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14027..14110)
                     /locus_tag="IVY71_001840"
     CDS             complement(14027..14110)
                     /locus_tag="IVY71_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP7985891.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5017 a         2221 c         2926 g         4017 t
ORIGIN      
        1 ccttgaaggt gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga
       61 aagattatct aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga
      121 aactgccctt aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgtt
      181 tgactgtgga aaagaatttt ctaactggaa agccattagt aaccaacatg atattgatat
      241 atattttgcg gaccctggaa caccttctca acgcccatta aacgagaatt ctaacgggat
      301 tctgcgtcgt aatggactgc cgaaatcaat ggattttaga aaagtgaatc agacatttat
      361 ttccagtgtc agcaatcaac gtaatcatat tccaagaaaa tcattgaatt acagaacacc
      421 aatcgaaata tttttgagct atgtacaaga agcattttat tctagcttaa tttgacaaat
      481 catactatga tacgtgtttg tttttctgtg ctgtttagtg aatgattagc agaaatatag
      541 agagtaagat tttaattaat tattaggggg agaaggagag agtagcccga aaacttttag
      601 ttggcttgga ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc
      661 gaagcgaaca cttgatcttt taagttgcta tctttttaag gtcaatagag tatacttatt
      721 tgtcctaata tggttttagc agtataattg acttggtgaa taggtcattt aagttgggca
      781 taataggagg agtaaaatga aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt
      841 ggctattttt aatgagcaac aatatgcgca agattttatc gcttacgaaa agacaatttc
      901 tgataagcaa tttgaaattg aaaaagtaga tatagctgat tggttattgc aaccgagaga
      961 attttagagg ttggttgaaa atggctaaaa ttggttatgc acgtgtcagt agcaaagaac
     1021 agaacttaga tagacaactg aaagcgttag agggcgtttc taaggtcttt tcagacaaat
     1081 taagcggtca atcggtcgaa cgcccacaat tacaagctat gcttaactat attcgtgaag
     1141 gggatatagt tgttgttact gaattagatc gattaggacg aaataataaa gaattaacag
     1201 aattgatgaa tcaaattcaa attaaggggg caaccctgga agtcttaaat ttaccctcaa
     1261 tgaatggtat tgaagatgaa aatttaagac ggctgattaa taatttagtg attgaattgt
     1321 ataagtacca agcggaatct gaacgcaaac gaattaaaga acgccaagcc caaggaattg
     1381 aaattgctaa gaaaaaagga aaattcaaag ggcgacaacc taaattcaaa gaaaatgatc
     1441 cacgtttaca acacgctttc gatttatttt tgaatggttg ttccgataaa gaagttgaag
     1501 aacaaactgg aattaatcgc cgaacgttta gaaggtatcg atcaagatac aacgtgacag
     1561 tagatcaaag aaaaaaacaa tgaaaagagg gatagttaat gagtacggtt attttagctg
     1621 aaaaaccaag ccaggcatta gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg
     1681 gttattttga gatcaaagac ccactattta cagatgaaac gtttatcacc tttggttttg
     1741 ggcatttagt cgaattagca gaaccaggtc attataacga aaagtggcag aactggaaac
     1801 ttgaatcttt gccgattttt cctgatcgat acgattttga agttgcaaaa gataagggaa
     1861 agcagtttaa aattgttgct gaacttctca aaaaggcaaa tacaattatt gtcgcaacag
     1921 atagcgacag agaaggtgaa aatatcgcct ggtcgattat ccataaagca aatgcctttt
     1981 ctaaagataa aacgtataaa agattatgga tcaatagctt agaaaaagat gtgatccgaa
     2041 gcggttttca aaatttgcaa ccaggaatga attactatcc cttttatcaa gaagcgcaaa
     2101 cacgccaaat tgccgattgg ttgatcggca tgaacgcaag ccctttatat accttgaatt
     2161 tacaacaaaa aggcgtacaa ggtacatttt cattaggacg tgtacaaaca ccgactttat
     2221 atctcattta tcaacgccag gaagcaatag aaaactttaa aaaagaacct tttttcctga
     2281 ataattcata gttttactaa tagtctttcc taacattgat ttaatagtct ttaaagtatt
     2341 ctgcttcatc acccattagg tgatgaaaaa agaacccctt tctgatttag cggacaaata
     2401 ctttgaccta atctcagaag atataaaaga aaaaataata aaatatccga tttatacttt
     2461 gtataaagaa ctaaagtagt tagcaaatca tttacagcaa atcactatgt tccacatacc
     2521 attttacata cttaccttat ccacgacaca tgttgagata gtatcgaaga taaagaaagt
     2581 tgctccatgt tgtttttgcg agatggggaa atcaaagcca cattgacaac catgatgaat
     2641 aagcttgctt tcagtcataa gctaatccta gaaccacttt tcaagtcagt tagccaaatc
     2701 gatgaagaaa gcgaccgtga aagaatggac gctattgata agctaatgga gcaactattg
     2761 gaagaacgca atacccttat tgcactgatg tccaaaggtt tcctagaacc tgctcttttt
     2821 aatcaggaac gaaatgtttt agatagtgag attaaaaatc ttactaccga aaaaacaaac
     2881 ctggtaacga actctgcgag tggggttttg cgagcaaacg agataaagga cctcattaat
     2941 tatgtgtcag cagataattt taatggtgac tacacggaag aactatttga agaatttgta
     3001 gtgaacatca ttgtaaattc cagggatgag ctgacattca atttgaaatg cggtctttcc
     3061 ctgaaagaaa aggtggtgag ataaatggca tatattccat atggatacaa aattcaagat
     3121 ggagtggtta ctgtcgatga aaaggcagca ggtcaagtaa aggtattctt tgagaaatac
     3181 atatcaggac tatcccttac agtggctggc gaacaggcag gtattgaaaa gacacattct
     3241 gtgatggggc gcattttgaa aaacgtcaac taccttggaa atgatacgta tccagcaatt
     3301 attgataaag agatatttga taaagctgaa gaagttagag ataaacgtgc aaaggattta
     3361 ggacgagtgg tagagcttgc cgctttcacc tctccccctc ccaaagaacg attcaaaatg
     3421 aaaaaggcag ataataagat gccagtggat ccttttgaac gagcagaata cttatacagt
     3481 ctgatagaaa gcgaggaata aagtgacaga gaaaaatata atggttattc ctgctcgtaa
     3541 aagagtagga agtacagccg caaaagaaaa gataaagaaa cttcgtgttg ctgcctattg
     3601 ccgtgtttct acagaaacag aagaacaaaa ttctagctat gaggttcagg tctcacacta
     3661 tacagagttt ataaagaaaa ataatgaatg ggagtttgct ggcatctttg cagatgatgg
     3721 tatctctggt acgaacacta aaaagcgtga cgaatttaat cgcatgattg cagagtgtat
     3781 ggacggtaac atcgacatgg ttattacaaa atccatcagc cgatttgcgc gtaacaccct
     3841 agactgcctt caatacatta gacagctcaa ggataagaac atatcagttt attttgagaa
     3901 agagaacatc aacaccacgg atgccaaagg tgaggttttg ctgactatta tggcatcttt
     3961 ggcacagcag gaaagccaga gcctttcaca aaacgttaag cttgggctac agtaccgata
     4021 ccaacaagga aaggtgcagg tcaaccataa gcgatttatg ggctacacga aagatgaaga
     4081 tggaaattta actattgtcc ccgaagaagc tgagattatc aaacgcatct accgagaata
     4141 ccttgagggt cagagtctag tgggcattgg tcgagccctt gaaaaggatg gtattttaac
     4201 agcagcggga aaaccaagat ggcgaccaga atcagttaag aagatacttc aaaacgaaaa
     4261 atacatcgga gatgcccttc tgcaaaagac tgtcacagta gattttctga ccaagaaacg
     4321 agtgaaaaat gaagggcatg ttccccaata ttatgttgaa aatagccatg aagcgattat
     4381 tcctaaagag ttatttttgc aggcgcaaga agaaattcat cggagaagca atatctacac
     4441 aggagaaggc aagaacaaaa gaatttacag tagcaagtac gctttaagtg ccatcacctt
     4501 ctgtggagat tgtggcgata tttatagacg ggtctattgg aatatccacg gaagaaaaga
     4561 atttgtctgg cgatgcgtga ctagaatcga gcaaggtcct gaagtttgta agaaccgaac
     4621 cgtaaaagaa gatgaacttt atggtgcagt aatgactgcg attaataagc tacttgcagg
     4681 tgggaacaat atgataaaga cactggaaga gaatattcat gctgtgattg gcgaaaccac
     4741 agaataccaa atttcagaga ttaacaactc actagaggaa aaacaaaaag aactaatcaa
     4801 gctggcgaac aagggtcaag actatgacca cttagcagat gagattgatg agctgagaga
     4861 caagcgacag atccttttag tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa
     4921 tgagctgatt gaatttatcc gcaagaacaa attccgcacc ttagaatatg atgataagct
     4981 tgtaaggaag ataatccaga gcgttacagt ctatgaaggc cacttcgtca tagccttcaa
     5041 atctggcatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctacagtag
     5101 agttgtgggt tttcttttgc ttgctgatta taataatatt ttaataacta ttgtgtttat
     5161 caaccaaatg gtttataata ttcttagcgt aaacttggag gtgctgtatg actacggcag
     5221 aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta
     5281 ttggccagac tccacagaat ttcaataaaa aattacaacg agaaacagta accttggatg
     5341 agttgaaggc cattgctgat gtgctaggtg tcaagtttgt gcaggcattt attttaccag
     5401 atggagaaga aataaaaata tctaacgaat aaaggaggat gtttatatgg taggtaatgt
     5461 tattcaaata gtaacagaga aattatcatc tttgcctttt atagaaggca tcgtattagg
     5521 gggctcacgt gcaagaggca cccatacaga aaattctgat atagatatcg gaatctatta
     5581 caattcagac tcatttgacc tgacagcgat taatcaaatt gctacagagt tggatgatga
     5641 gaatagaaac aaccttgttg tacctcccgg agcgtggggt gattgggtta atggcggtgg
     5701 atggttagtt ataaacgggt gtcatgttga cttgatatta cgtgatataa aacgggtgga
     5761 acaaataatc aaagatacgg agcaaggaat tgttactgcc aattatcaga ccgggcatcc
     5821 ccatggttat ataagtgcta tgtatcgtgg agaattagcg attagtaaaa tactatatgc
     5881 taagaacgaa agcttatgcg aattaaaaaa gcaggcagaa atttacccta ctgctctaaa
     5941 gaaaagcttg atgaactttt ttatatttga agctgaattc tctttaatgt ttgtaaaagc
     6001 aaatgcgggg gcagaggata aatattatat tgcaggccat gtttttcgta ttatttcatg
     6061 cttaaatcaa gtactatttg catgtaataa tgcttattgc attaacgaaa agaaagctat
     6121 aaaactgctt gaaacttttg aatataaacc tgaaaaatat gccgaaagag ttaatcatat
     6181 ttttgaagta ctcggtcttt ctctttttga atgctacgac atgaccgaga aactttataa
     6241 agaagtgaat gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga
     6301 aagaaaacaa atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc
     6361 agaaaggact ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta
     6421 agggtaagcg tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg
     6481 aaaacggtgc ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa
     6541 aaggaaaaac ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt
     6601 tcccagagga gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact
     6661 atgagaattt aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta
     6721 cagttgaaca tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag
     6781 gagaaatact gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt
     6841 ttttggaaga aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc
     6901 tttcaaatag ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg
     6961 atattccggg gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa
     7021 agaagatgta ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga
     7081 aatgatggat ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct
     7141 tgaggggtca cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac
     7201 atattttgta agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg
     7261 gaatataata atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg
     7321 attttcctat cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt
     7381 ggaagagtta gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga
     7441 ttgtagaatt aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag
     7501 cgcaagggag tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa
     7561 aggattgtgc cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca
     7621 tgagctgctg agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt
     7681 aggcaagaac tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt
     7741 gtccacctac cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca
     7801 attgttcagg gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta
     7861 tgataggaat ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg
     7921 ctgcctggat agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa
     7981 atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt
     8041 aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg
     8101 ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt
     8161 gaatatattg acaatactga taagataata tatctttact accaagacga taaatgcgtc
     8221 ggaaaagtta aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta
     8281 tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg
     8341 gcaaagcata aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct
     8401 tgtaaattct atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc
     8461 aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg
     8521 aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt
     8581 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
     8641 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
     8701 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
     8761 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
     8821 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
     8881 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     8941 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     9001 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
     9061 atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg
     9121 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
     9181 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
     9241 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
     9301 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
     9361 aaataaaata ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa
     9421 aagctttaac tacaagcttt ttagacatct aatcttttct gaagtacatc cgcaactgtc
     9481 catactctga tgttttatat cttttctaaa agttcgctag atagagttct atattagaga
     9541 tgtaaagagt tttggtgaag aggtttgtga gtatggcttt tataaccagg gtgcaatgag
     9601 aagcactaac agcagctagc ttaagaacgc ataggatata ctttttatgg aagtcacaga
     9661 ggaggaataa aatttgaaca ggattaacag agtaacctct attcttattc agctgcaatc
     9721 aaaaaaaata attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt
     9781 ttacagagat atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa
     9841 aggatatttt cttgtagagg gcttcctact tccgccggta atgtttacag cggcggaagt
     9901 gggtgcattg attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa
     9961 ggattttgat tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta
    10021 tgcacaggag ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt
    10081 ggcggataac gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat
    10141 tcaatatcct gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact
    10201 ggggttttat gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg
    10261 aattttcgtg tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat
    10321 gatggctctt tggaacaaat attaaaacaa atgctttcat ataaaaaact atatgcccta
    10381 caatgccgtt gtaaaaattg gggaaaaggg atatattgat tttatatctt tctcaaaata
    10441 aatataaaag ccctttaaag agggctttta tatattaatc acaaatcact tatcacaaat
    10501 cacaagtgat taatcacaaa tcacttgtga tttgtgattc ttaatgatac aatattacta
    10561 tacaaaaaaa gaatggggcg tagttatgga gaaggaaaaa ctaaaaatac ttgaagaatt
    10621 aagacgtatt ttaaacaata aaaatgaagc aattattatc ttgaataatt actttaaagg
    10681 tggtgttgga aagtccaaat tatcgactat gtttgcttac ttgacagaca aattgaattt
    10741 aaaagtttta atgatcgata aggacttaca ggcaacattg acaaaagact tagcaaaaac
    10801 ttttgaggta gaattgccac gtgtcaattt ttatgaaggc ttgaaaaatg gaaacttggc
    10861 ttcttctatt attcatttga ctgataattt agacttgatc cctggcacgt ttgatttgat
    10921 gttactgcca aaattaactc gctcatggac gtttgaaaat gaaagtagat tgcttgctac
    10981 tcttttagca cctttaaaaa gtgactatga tcttattatt attgatactg taccaacgcc
    11041 aagcgtttat acaaataatg caatcgtggc gagtgattac gttatgatcc ctttacaagc
    11101 agaagaagaa agtacaaaca acattcaaaa ctatatttcc tatttgattg atttgcaaga
    11161 acagtttaac cctggactag atatgattgg ttttgttcct tatttagttg atacggacag
    11221 cgcaacgata aaatcaaacc tggaagaact gtacaaagaa cataaagagg ataatttggt
    11281 tttccgaaat attatcaagc gaagtaataa agtaagtact tggtctaaaa atggcattac
    11341 agaacacaaa ggctatgaca aaaaagtttt gtctatgtat gagaacgtat tttttgaaat
    11401 gattgagcga atcattcaat tagaaaatga aaaagaatag aatcacaaat cacaagtgat
    11461 taatcacaaa tcacttgtga tttgtgatag gtgatgataa aataaatagt aagaagaaat
    11521 agaaagaagt gagcgatcgt gggaaattta ggcgcacaga aagcaaaacg aaatgatacg
    11581 ccaatcagcg caaaaaaaga tataatgggg gataagacgg ttcgtgttcg tgccgacttg
    11641 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggat
    11701 caagccttag aagaatatat acggaaatat ttacctgaca aactttaaaa ggagtgaaaa
    11761 tgaaatggca gttacgtatg aaaaaacatt tgaaattgaa atcattaatg agttatcggc
    11821 aagtgtttat aatcgggtat taaattatgt tttgaatcat gaactagata ctaaaaatac
    11881 tcgtttacta gaagtgaatc ttttaaatca attagaagtg gcacaagaag ttgatttatt
    11941 tcaacaacca tttgaagaat tacaagctat tcatgagtat tggcggtcaa tgaatcaata
    12001 ttcaaaacaa attttgaata aagagaaagt ggcttaacat ggcgaatatt actgacttca
    12061 ccgaaaagca atttgaagat cgtttagaaa agaatgttga acgactaact aaaaatagac
    12121 tagcggttga atcgccaacc gcttttttac ttggtgggca accagggtca gggaaaacca
    12181 gtttgcgatc agcaattttt gaagaaacac aagggaatgt tattgtcatt gataatgata
    12241 cctttaaaca acagcaccca aattttgacg aattagtgaa actctatgaa aaagacgtag
    12301 taaaacacgt taccccttat tcgaatcgca tgacagaagc acttattagc cgtttaagcg
    12361 atcaaggcta taatttagtg attgaaggaa caggacgaac aacagacgtt cccattcaaa
    12421 cagccacaat gcttcaatct aaaggttatg aaacaaaaat atacgtcatg gcagtaccga
    12481 aaattgaatc atacttagga acgattgaac gatatgaaac catgtatgca gatgatccaa
    12541 tgacagctag ggcaacacca aaacaagcgc atgatattgt tgtcaaaaac ttaccgacca
    12601 atttagaaac ccttcataaa acgggcttat ttagcgatat aaggctttac aatagagaag
    12661 gagtgaaact ctattcaagc ttagaaacgc cttccattag tccgaaagag accttagaaa
    12721 gagaattaaa tcgtaaagta tcagggaaag aaattcaacc gactttggag agaatagagc
    12781 aaaaaatggt tcaaaatcaa caccaagaaa cccctgaatt taaagcaatt caacaaaaaa
    12841 tggaaagctt gcagctacct acaccaccaa tacccaaaac acctaaactt ccaggtattt
    12901 aaaccttaaa aaaggaaaag agtagttacc aaaaaacggt aactactctt tttttataaa
    12961 aacatttcct catgttgggg tcccgagcgc ctacgaggaa tttgtatcga taagaaatag
    13021 atttaaaaat ttcgctgttt ttttctaatt tcattttgag gtgaattaat tggtagttat
    13081 tagaggtgcc ctatacctaa taatttatct acattccctt tagtaacgtg taactttcca
    13141 aatttacaaa agcgactcat agaattattt cctcccgtta aataatagat aactattaaa
    13201 aatagacaat acttgctcat aagtaacggt acttaaattg tttactttgg cgtgtttcat
    13261 tgcttgatga aactgatttt tagtaaacag ttgacgatat tctcgattga cccattttga
    13321 aacaaagtac gtatatagct tccaatattt atctggaaca tctgtggtat ggcgggtaag
    13381 ttttattaag acactgttta cttttggttt aggatgaaag cattccgctg gcagcttaag
    13441 caattgctga atcgagactt gagtgtgcaa gagcaaccct agtgttcggt gaatatccaa
    13501 ggtacgcttg tagaatcctt cttcaacaat cagatagatg tcagacgcat ggctttcaaa
    13561 aaccactttt ttaataattt gtgtgcttaa atggtaagga atattcccaa caattttata
    13621 cctctgtttg ttagggaatt gaaactgtag aatatcttgg tgaattaaag tgacacgagt
    13681 gttcagtttt aatttttctg acgataagtt gaatagatga ctgtctaatt caatagacgt
    13741 tacctgttta cttattttag ccagtttcgt cgttaaatgc cctttacctg ttccaatttc
    13801 gtaaacggta tcggtttctt ttaaattcaa ttgttttatt atttggttga gtactttttc
    13861 actcgttaaa aagttttgag aatattttat atttttgttc atgtaatcac tcctgaagtg
    13921 attacatcta taaataaata cagaagttaa acgatttgtt tgtaatttta gttatctgtt
    13981 taaaaagtca taagattagt cactggtagg aattaatcta acgtatttat ttatctgcgt
    14041 aatcactgtt tttagtctgt ttcaaaacgg tagatgtttt atctacatta cgcatttgga
    14101 ataccaacat gacgaatccc tccttcttaa ttacaaattt ttagcatcta atttaacttc
    14161 aattcctatt atacaaaatt t
//
LOCUS       DACCXW010000073        14143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCXW010000073 DACCXW010000000
VERSION     DACCXW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.074"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..319
                     /locus_tag="IVY71_001841"
     CDS             <1..319
                     /locus_tag="IVY71_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7985892.1"
                     /translation="PKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLN
                     EHSNGLLRKDGLPKEMDFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKF
                     CLA"
     gene            complement(497..2494)
                     /gene="tkt"
                     /locus_tag="IVY71_001842"
     CDS             complement(497..2494)
                     /gene="tkt"
                     /locus_tag="IVY71_001842"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7985893.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2596..2847)
                     /locus_tag="IVY71_001843"
     CDS             complement(2596..2847)
                     /locus_tag="IVY71_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7985894.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2994..3617
                     /gene="lexA"
                     /locus_tag="IVY71_001844"
     CDS             2994..3617
                     /gene="lexA"
                     /locus_tag="IVY71_001844"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7985895.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3797..4015
                     /locus_tag="IVY71_001845"
     CDS             3797..4015
                     /locus_tag="IVY71_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985896.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4304..4816)
                     /locus_tag="IVY71_001846"
     CDS             complement(4304..4816)
                     /locus_tag="IVY71_001846"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7985897.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4830..7127)
                     /gene="recJ"
                     /locus_tag="IVY71_001847"
     CDS             complement(4830..7127)
                     /gene="recJ"
                     /locus_tag="IVY71_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7985898.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7269..7703)
                     /locus_tag="IVY71_001848"
     CDS             complement(7269..7703)
                     /locus_tag="IVY71_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7985899.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7713..8501)
                     /locus_tag="IVY71_001849"
     CDS             complement(7713..8501)
                     /locus_tag="IVY71_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7985900.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8498..9439)
                     /locus_tag="IVY71_001850"
     CDS             complement(8498..9439)
                     /locus_tag="IVY71_001850"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7985901.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9616..10203)
                     /locus_tag="IVY71_001851"
     CDS             complement(9616..10203)
                     /locus_tag="IVY71_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7985902.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10424..11737)
                     /gene="obgE"
                     /locus_tag="IVY71_001852"
     CDS             complement(10424..11737)
                     /gene="obgE"
                     /locus_tag="IVY71_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7985903.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            12025..13035
                     /gene="gap"
                     /locus_tag="IVY71_001853"
     CDS             12025..13035
                     /gene="gap"
                     /locus_tag="IVY71_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7985904.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(13120..14055)
                     /locus_tag="IVY71_001854"
     CDS             complement(13120..14055)
                     /locus_tag="IVY71_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7985905.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3933 a         3161 c         2485 g         4564 t
ORIGIN      
        1 acccaaaaat cttttcaaat caattacctt tgattgtgga aaagaattct ctaattggaa
       61 aagtattagt aatacgaatg atattgatat ttatttcgca gacccaggaa cgccttccca
      121 acggggatta aatgaacatt caaacggact cctccgaaaa gatggactac caaaagaaat
      181 ggacttcaac caagtcaatc aaggattcat ctcatccgtt gcgtccaaaa gaaaccatat
      241 ccctagaaaa tcactaaatt accaaacacc attagaagtt tttttgagtt acgtaaatgg
      301 aaagttttgt ctcgcttaat ttgacaaata atattctcaa gaaacacaaa ctaatccgaa
      361 gaattctttg ttttgtcatt taaatgtaac atagccgaaa acgaaaagta atattgattt
      421 attcttctaa cacataaaga acagctgtct ccaacttgca gacagctgtt cttatcagtc
      481 aaacaattgc ttggatctat gatagttgat tgtacgtatt cacgacattt tctactgtaa
      541 atccaaattc ttctaaaatt ttagtgccag gggcagatgc accgaaatga tcgattgtaa
      601 tcgttgcgcc ctctgttcct acgtagcgtt cccatccaaa tgatgcagca gcttcgatag
      661 ccacacgttt cttcactgat ttaggaagta cactttcttt atattcagaa gattgttttt
      721 caaagaggtc aaagcttggc atagaaacaa cagaaacatc tttgcctttt tcagccaatt
      781 ctttttgagc tttcacagcc aaatcaactt cagaacctgt tgcaatcaaa ataccttctg
      841 gtgtttctcc ttgtgatgga gaaagaacat acgcgccttt tttcaccatt tcatctgcta
      901 cttcttttgt agaaggcaat actggaaggt tttgacgact caatacaagg atagtaggcg
      961 catctgttgt ctccatcgct actttccaag cagcacgtgt ttcatttccg tctgccggac
     1021 gaatcacttg aacaccaggc atgcagcgaa cagatgctag ttgttcaatc ggttcgtgcg
     1081 ttggtccgtc ttctccgaca gcaaccgagt catgtgttaa gacgtaaaca acaggagtat
     1141 gttggattgc tgctaaacga actgctggtc ttagataatc gacaaagaca aagaatgttc
     1201 caccgtagat acgagttccg ccatgaagtt ggataccatt catagccgaa gccattgcaa
     1261 attcgcggac accgaaccag atgttgcgtc cttcataatg ttcaggagtg aaatctttat
     1321 ctgcagccac cattgtatta ttggaacctg acaagtccgc tgaaccaccc cagaagcttg
     1381 gtatagcttt cgataattct tggatgactt ctttacttga aacacggctt gcttgactgc
     1441 ttccaacttc ataagttggc aattctgcat cccagttttc tggtagtttg ccatcaaaag
     1501 catcttcaaa ttgttgtgct aattcaggat aagctttttt ataattggca aacatttcat
     1561 tccatgcatc ttcagctttt tgtccttctt ctatcatcgt ttgatggaag cgggcagcta
     1621 cttcttctgg aacagtgaaa tctggatact cccaaccata aacttcttta gccatcttga
     1681 tgccatcttc accaagagga gcaccatgaa cagcagatgt tccttcttta ggagcaccat
     1741 agccgatgac tgtttttact tcgatcaatg ttggtttgtc tgtttcagct tttgcttctt
     1801 caatcgcttt agagattgct tctaggtcat ttccgtcttt cactaagata tgttgccagc
     1861 catatgcttc atagcgagca ccaacgtttt ctgtgaaagc ttttgaagtt ggaccgtcta
     1921 gtgaaatatc gtttgaatcg tataaaacga tcaatttccc tagtttcata tgacctgcca
     1981 tcgaacttgc ttcttgagat accccttcca tcaagtctcc gtcaccgcat attgcatacg
     2041 tatagtgatc catcacatta aagttttctt tgttatacgt tgctgcaagg tgcgcttctg
     2101 ccattgccat accgacagcc attgcaatcc cttgtcctaa tggccctgta gtggcttcta
     2161 caccatctgt atgatgtact tcaggatgtc ctggtgtttt agaatcccat tgacggaatt
     2221 gtttcaaatc atcgattgtc acttgatagc cagctaaatg aagcaagcta tacaacattg
     2281 ctgacccatg tcctgccgaa agaacgaaac ggtcgcggtc agcccagttt tttgatgtcg
     2341 ttggatttac tttcaggtgt tttgtccata acgcatatgc cataggtgct gcgcccatcg
     2401 gcaatccagg atgtcctgag tttgcttttt ggactgcttc aatacttaat gtacgaattg
     2461 tgttaacgcc aagttggtca attttgtcaa acaaaataac ccctcctatt aatttaggtt
     2521 ttttttacct ttatttctta cactttcatt ctataagatg tcacacgaaa aagcaatctt
     2581 tttcagtatt ttttactaaa agttgtcatt tcggttgtgt aagccttttt tcttttggat
     2641 ttcttttaat ttttccgggg tcacgtcatt gccctcagga tcgacgactt tcattccttc
     2701 gatatgatga cgcataccgg aacgaaaagc ttccaaatac tctttgcgca agttgatttg
     2761 ttcttgttct tcggcttttg agagaccatt ttccttcttt ttacgtgcaa gttcattgat
     2821 ccgctctatt ttttcaggtg acagcatgct cagccctcct tttactctcc ttcatattat
     2881 ataaaaactg aaaaaaaaac aactagataa actaaataaa atacgaacat tcgtttgatt
     2941 tagcgctttt tatatggtac tatttattta tcaagtgaaa gaaggtgtac cttgtggccc
     3001 gacaaactga atcaagacaa atcgaagtct tacgatatat ccatgaacaa gtaacccaaa
     3061 aaggataccc gccgactgtc cgtgaaatcg gggaagctgt tcagctttct tctacttcta
     3121 ctgttcacgg tcatcttgca cggcttgaaa aaaaagggtt tatccaaaga gatccgacga
     3181 aacctcgtgc aatcgagttg acaaaacaag gtttagaaaa gattgggatt cgtccaacaa
     3241 ctattccagt acttggcgtc gtaacagctg gagaacccat tttagcagta gaggatgctt
     3301 cagacttctt tccaatccct cctaatcttt cttccgaaga aggcagtctc tttatgctga
     3361 caattcgtgg agatagtatg atcaatgctg ggatattgga tggtgacaac gtgatcgttc
     3421 ggaagcaaga aactgctcaa aatggcgata ttgtcattgc aatgactgcc gaggacgaag
     3481 cgacttgcaa acgattctat aaagaaaaaa actttttccg tctgcagcct gaaaacgact
     3541 tgctcgaacc aatcatctta gatcaagtat ctatattagg acgagtagta ggactttatc
     3601 gtagtcatat ctattaattg ctactaatta aaagaaaact ttttaaaaag agtttgagac
     3661 gttggtctca aactcttttt tcgcccgggt tcttcttctt tcaaattttt atcacttccc
     3721 tctttacaaa ccgaacaaat attcgtatac taaatatacg aacaattgtt cgcataaatt
     3781 aaagaggtga gaattcatga aatcaatgag acgtcttggc ttagtattaa ctgttttgtt
     3841 tttaggtatt attattggta atttcggcat acgtccagct ttacttgtgt ttgttccgtt
     3901 atttactttg tggttcatgt tgtgggatga aaagaagtac cgtcaatctg agcggcgcag
     3961 aattcatgaa gatcatttct atcatgagcc ctactacaga tccaggcaat cataataagg
     4021 attgactgct aattattcaa aagacaagaa gttaaacttt tacgggggtg tgacaaaaat
     4081 cggtcacatc ctttttttcg aataaacggt gttctaaagc cagtttttcg gtattaagtc
     4141 aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt ccatttataa
     4201 aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc gctttaaaat
     4261 cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat tccattaatg
     4321 taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat tcgatcaaaa
     4381 atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc gctttgattg
     4441 ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt ttaatggcat
     4501 ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact tcgatggttt
     4561 ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac tcataagcaa
     4621 caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca attcgttttt
     4681 cttttgcata atttacgatt tgtttagtag cttcccgata agcgtcacca tctgccatca
     4741 gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata ctggcaatat
     4801 aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt tatggtagga
     4861 atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgtcg ctggtataat
     4921 ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac accgttctct
     4981 attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa tttttcaggg
     5041 atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg ttcttgagaa
     5101 tggatcaatt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt caaataggcc
     5161 tcatcaggtg aaatccccat cagataaaca cgattgaatg aataagaccg aaaaacttct
     5221 ttcagatccg ctaaatcatc tggacaatcc ataacaacta gagagtttgg ttttcttttt
     5281 tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc ctggataatc
     5341 tcaggcacga atttcaacgt cgaagaatca aatgctaaat aaaggctacc ttccgacgtt
     5401 cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc acccacagaa
     5461 aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat cgaaagcttt
     5521 cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa accaaaagcg
     5581 accgcatcaa gctgactcga tgcatcggaa aggacaaatt tcagatgctg ttggtttgct
     5641 ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg caaaggatta
     5701 tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt tacttcattt
     5761 ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat ttgatgatcg
     5821 actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg catcgttaaa
     5881 ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg gtcaagcatt
     5941 tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc atcttctttt
     6001 aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc aacaatgccc
     6061 aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc cttattgacc
     6121 atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc attgatctca
     6181 ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag taacgagacg
     6241 gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat cgaaaaaccg
     6301 atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag cgcctgaagt
     6361 cctatccgtt ctgaatgatg aatggcatcg attcctaatg aaacaaggat tcgattctca
     6421 tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa aaattctgca
     6481 ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc tgccaaatct
     6541 ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc gtctggtaaa
     6601 ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg ctgagcgtat
     6661 gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg gattccttct
     6721 tcgatctttt cttggtatac cgattggttt ggtccatacc catgctcaaa tcgatttggc
     6781 aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac cgttgcactg
     6841 gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact gattacagct
     6901 tcttggattc ggctgattgc tttatccata tcgtgtatca gatacggatc atgtaattct
     6961 tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc tctttgccac
     7021 agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag aaaggattca
     7081 gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt ctcactccta
     7141 acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga ataaaaacag
     7201 atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc atttatgaaa
     7261 tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt ttgttcttcc
     7321 ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg ataagcagct
     7381 tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc ttcttcaatt
     7441 ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt gatttgcttt
     7501 ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc aatcagcgca
     7561 gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa acttacagga
     7621 acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac aaatccaaga
     7681 atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat tccgctggct
     7741 aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc taaaataaac
     7801 ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt tgccaacttg
     7861 gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg atcagcctta
     7921 tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg taacagctac gccaagtggt
     7981 ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt ggcagaataa
     8041 accgttgatt ttgcagttgc catttttcca gccatagagg cgatattaat gatatgacct
     8101 tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat tcctaagatg
     8161 ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc ttcgaaaatc
     8221 ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat caccttttca
     8281 tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata agcctctttg
     8341 ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct tcgtgcgcaa
     8401 cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc cagaccgcct
     8461 gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc tcctcgcgaa
     8521 atggaatctc aatgctatca aaatctttca taatcgtcgt gttttcaaac acttcttgtg
     8581 cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata tgagtaagta
     8641 gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc gtggaatgga
     8701 aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt accagaatat
     8761 cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct aaaatagtca
     8821 cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc cgcccatctt
     8881 ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct ggaataccta
     8941 attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc acacgataac
     9001 caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc tgatcagtaa
     9061 aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa cgcgtttgcg
     9121 aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa atctctaatg
     9181 gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca aaaatatgat
     9241 cgccgtgtaa atgtgtaatg aaaattttac cgatttttct cggacgaata gtcgttcgca
     9301 aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg cgctcgtcta
     9361 aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct gcacctgttc
     9421 ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt aattttaggg
     9481 taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa ataacgttct
     9541 aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc gtttgagatc
     9601 ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg aagtttgcgg
     9661 atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag cttctttcgc
     9721 atcatgcaca tctttttcga catcttcata atccaagcga gtaatttctc cgcctaattc
     9781 catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca agatcagtcc
     9841 tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg catcttttat
     9901 ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta ataaaatccc
     9961 tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag aagatttgtt
    10021 attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat taggatcatg
    10081 gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact ttttgatttc
    10141 tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta ctcgtttgct
    10201 cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat tgaaaaataa
    10261 gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt cgtatcacag
    10321 ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca acctccctta
    10381 aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg acaaattcaa
    10441 attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc cgaagagctt
    10501 catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca tcatgatcga
    10561 aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac gttgcatctg
    10621 aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt acagtttctt
    10681 cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa tctgctgtag
    10741 cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat actgggatgt
    10801 catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc actagatttt
    10861 ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc tctagcaagc
    10921 gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa ggatctcgtc
    10981 cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg atatgacgaa
    11041 ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct ggcaagtctg
    11101 ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggt acgagcgtcg
    11161 tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt aaagtcgatt
    11221 tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat tctagttcga
    11281 tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc cggtgcagga ttccgaggag
    11341 aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact agtgtttgac
    11401 cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca gtggttcctt
    11461 gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta ctcatcccat
    11521 tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat gtacgtaacc
    11581 cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca gccggtccgc
    11641 cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc ttgccagctt
    11701 tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc tattattcat
    11761 ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat tttatcggtg
    11821 aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct ttttttatca
    11881 tttggtaagg tttccaacct tttttctgct accaagtata attttttgac aaaatattct
    11941 ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt tcacagcacc
    12001 cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg atttgggcgt
    12061 attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga agtagtagca
    12121 atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga ctctactcat
    12181 ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga tggcgaagca
    12241 actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga aaacggtgta
    12301 gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa agcgcattta
    12361 gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa aaccatcgtc
    12421 tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc aggttcatgt
    12481 acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg gatcgaagtt
    12541 ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga tggacctgta
    12601 aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc acattctact
    12661 ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt acaaggacac
    12721 gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt attgaaaaca
    12781 aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga caatccttca
    12841 ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac tcaaggttcg
    12901 atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact tgttaaaact
    12961 gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt actagaaaaa
    13021 ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact ggatccagtc
    13081 aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat attggcttaa
    13141 aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt ccgcatgctc
    13201 ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga ccttatctaa
    13261 catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg ggcgctggtt
    13321 tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac ggacttttgt
    13381 cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat cgtctagctg
    13441 ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg ctttcacatt
    13501 atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc tgacaccgta
    13561 tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc actgtacata
    13621 gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc catgtcctct
    13681 tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt aattggtcat
    13741 cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac ttttcgtttc
    13801 agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg tccaatcgct
    13861 tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg tctgtgttgt
    13921 accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat aattataaaa
    13981 ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta aaaggatttt
    14041 catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac cttgaattag
    14101 tataatcaaa gaatctctac tatgcaatgt tcatttttat aac
//
LOCUS       DACCXW010000074        14139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCXW010000074 DACCXW010000000
VERSION     DACCXW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.075"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="IVY71_001855"
     CDS             complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="IVY71_001855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7985906.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="IVY71_001856"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="IVY71_001856"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7985907.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="IVY71_001857"
     CDS             8128..9096
                     /locus_tag="IVY71_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7985908.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="IVY71_001858"
     CDS             9114..10019
                     /locus_tag="IVY71_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7985909.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="IVY71_001859"
     CDS             10016..10822
                     /locus_tag="IVY71_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7985910.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10979..11947
                     /locus_tag="IVY71_001860"
     CDS             10979..11947
                     /locus_tag="IVY71_001860"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7985911.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="IVY71_001861"
     CDS             complement(12264..12788)
                     /locus_tag="IVY71_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7985912.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..>14139
                     /gene="scm"
                     /locus_tag="IVY71_001862"
     CDS             13075..>14139
                     /gene="scm"
                     /locus_tag="IVY71_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7985913.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4003 a         3047 c         2793 g         4296 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttggg aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggtaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatca
//
LOCUS       DACCXW010000075        13895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCXW010000075 DACCXW010000000
VERSION     DACCXW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.076"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IVY71_001863"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IVY71_001863"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7985914.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IVY71_001864"
     CDS             complement(1174..1869)
                     /locus_tag="IVY71_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7985915.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IVY71_001865"
     CDS             complement(1882..2598)
                     /locus_tag="IVY71_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7985916.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="IVY71_001866"
     CDS             2928..3536
                     /locus_tag="IVY71_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7985917.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="IVY71_001867"
     CDS             3861..4184
                     /locus_tag="IVY71_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7985918.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="IVY71_001868"
     CDS             4366..5736
                     /locus_tag="IVY71_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7985919.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="IVY71_001869"
     CDS             complement(5866..7167)
                     /locus_tag="IVY71_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7985920.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="IVY71_001870"
     CDS             complement(7521..9512)
                     /locus_tag="IVY71_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7985921.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="IVY71_001871"
     CDS             complement(9656..9922)
                     /locus_tag="IVY71_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7985922.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="IVY71_001872"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="IVY71_001872"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7985923.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="IVY71_001873"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="IVY71_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7985924.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /locus_tag="IVY71_001874"
     CDS             13266..13829
                     /locus_tag="IVY71_001874"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7985925.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
BASE COUNT         3987 a         2901 c         2564 g         4443 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaa
//
LOCUS       DACCXW010000076        13780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCXW010000076 DACCXW010000000
VERSION     DACCXW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.077"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            112..306
                     /locus_tag="IVY71_001875"
     CDS             112..306
                     /locus_tag="IVY71_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985926.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="IVY71_001876"
     CDS             575..874
                     /locus_tag="IVY71_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985927.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="IVY71_001877"
     CDS             complement(971..1405)
                     /locus_tag="IVY71_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7985928.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="IVY71_001878"
     CDS             complement(1637..2347)
                     /locus_tag="IVY71_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7985929.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="IVY71_001879"
     CDS             complement(2337..3197)
                     /locus_tag="IVY71_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7985930.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="IVY71_001880"
     CDS             complement(3202..3840)
                     /locus_tag="IVY71_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7985931.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="IVY71_001881"
     CDS             complement(3855..4154)
                     /locus_tag="IVY71_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7985932.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="IVY71_001882"
     CDS             complement(4197..5681)
                     /locus_tag="IVY71_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7985933.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="IVY71_001883"
     CDS             complement(5701..6162)
                     /locus_tag="IVY71_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7985934.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="IVY71_001884"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="IVY71_001884"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7985935.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="IVY71_001885"
     CDS             7465..8235
                     /locus_tag="IVY71_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7985936.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="IVY71_001886"
     CDS             complement(8508..9335)
                     /locus_tag="IVY71_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7985937.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="IVY71_001887"
     CDS             9903..11138
                     /locus_tag="IVY71_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7985938.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="IVY71_001888"
     CDS             complement(12137..12397)
                     /locus_tag="IVY71_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7985939.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="IVY71_001889"
     CDS             complement(12424..13725)
                     /locus_tag="IVY71_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7985940.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4429 a         2826 c         2172 g         4353 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgcatga caacgcaaag
//
LOCUS       DACCXW010000077        13757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCXW010000077 DACCXW010000000
VERSION     DACCXW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.078"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(374..1066)
                     /locus_tag="IVY71_001890"
     CDS             complement(374..1066)
                     /locus_tag="IVY71_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7985941.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1151..1327
                     /locus_tag="IVY71_001891"
                     /pseudo
     CDS             1151..1327
                     /locus_tag="IVY71_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1503..2072)
                     /locus_tag="IVY71_001892"
     CDS             complement(1503..2072)
                     /locus_tag="IVY71_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7985942.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2146..2469)
                     /locus_tag="IVY71_001893"
     CDS             complement(2146..2469)
                     /locus_tag="IVY71_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985943.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2623..3435)
                     /locus_tag="IVY71_001894"
     CDS             complement(2623..3435)
                     /locus_tag="IVY71_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985944.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3789..4637
                     /locus_tag="IVY71_001895"
     CDS             3789..4637
                     /locus_tag="IVY71_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7985945.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4782..5723
                     /locus_tag="IVY71_001896"
     CDS             4782..5723
                     /locus_tag="IVY71_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP7985946.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5713..7884
                     /locus_tag="IVY71_001897"
                     /pseudo
     CDS             5713..7884
                     /locus_tag="IVY71_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7996..10167)
                     /gene="copB"
                     /locus_tag="IVY71_001898"
     CDS             complement(7996..10167)
                     /gene="copB"
                     /locus_tag="IVY71_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7985947.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10187..12373)
                     /locus_tag="IVY71_001899"
     CDS             complement(10187..12373)
                     /locus_tag="IVY71_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7985948.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12373..12582)
                     /gene="copZ"
                     /locus_tag="IVY71_001900"
     CDS             complement(12373..12582)
                     /gene="copZ"
                     /locus_tag="IVY71_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7985949.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12595..13035)
                     /locus_tag="IVY71_001901"
     CDS             complement(12595..13035)
                     /locus_tag="IVY71_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7985950.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13109..13648)
                     /locus_tag="IVY71_001902"
     CDS             complement(13109..13648)
                     /locus_tag="IVY71_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7985951.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3891 a         2861 c         2424 g         4581 t
ORIGIN      
        1 tccatcattt tctctccaat cattttatta aaatcattaa caaaaatatt ataataggat
       61 ttaagtaagt tctaactaat cacatataaa aataaaaaaa catttgtatt ttatcaaagt
      121 tttattatac ttgtcaattt gtgtacatcc taaaatataa gctatttgtt tacttctgta
      181 gaatatatac tttgttgtta gataagttgc acatgtttat agaatctaaa taataaattt
      241 ttatgtaccg cccctcaaag tgaactgaac cccaaaagtt gaactattta atggactgtt
      301 tccgatattc tactggagat agtccattaa atagttcaac ttttggggtt cagttcaaag
      361 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
      421 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      481 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      541 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      601 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      661 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      721 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      781 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      841 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      901 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      961 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
     1021 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
     1081 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
     1141 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
     1201 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
     1261 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
     1321 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1381 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1441 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1501 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1561 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1621 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1681 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1741 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1801 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1861 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1921 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1981 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     2041 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     2101 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     2161 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     2221 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     2281 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     2341 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2401 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2461 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2521 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2581 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2641 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2701 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2761 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2821 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2881 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2941 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     3001 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     3061 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     3121 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     3181 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     3241 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     3301 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3361 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3421 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3481 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3541 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3601 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3661 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3721 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3781 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3841 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3901 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3961 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     4021 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     4081 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     4141 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     4201 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     4261 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     4321 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4381 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4441 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4501 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4561 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4621 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4681 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4741 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4801 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4861 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4921 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4981 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     5041 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     5101 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     5161 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     5221 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     5281 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     5341 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5401 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5461 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5521 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5581 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5641 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5701 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5761 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5821 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5881 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5941 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     6001 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     6061 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     6121 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     6181 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     6241 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     6301 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6361 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6421 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6481 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6541 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6601 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6661 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6721 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6781 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6841 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6901 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6961 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     7021 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     7081 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     7141 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     7201 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     7261 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     7321 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7381 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7441 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7501 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7561 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7621 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7681 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7741 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7801 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7861 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7921 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7981 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     8041 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     8101 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     8161 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     8221 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     8281 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     8341 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8401 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8461 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8521 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8581 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8641 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8701 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8761 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8821 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8881 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8941 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     9001 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     9061 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     9121 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     9181 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     9241 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     9301 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9361 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9421 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9481 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9541 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9601 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9661 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9721 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9781 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9841 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9901 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9961 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
    10021 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
    10081 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
    10141 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
    10201 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
    10261 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
    10321 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10381 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10441 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10501 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10561 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10621 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10681 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10741 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10801 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10861 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10921 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10981 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    11041 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    11101 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    11161 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    11221 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    11281 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    11341 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11401 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11461 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11521 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11581 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11641 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11701 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11761 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11821 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11881 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11941 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    12001 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    12061 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    12121 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    12181 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    12241 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    12301 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12361 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12421 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12481 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12541 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12601 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12661 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12721 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12781 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12841 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12901 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12961 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    13021 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    13081 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    13141 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    13201 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    13261 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    13321 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13381 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13441 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13501 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13561 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13621 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13681 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13741 gtcccttgtt tcgaata
//
LOCUS       DACCXW010000078        13649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCXW010000078 DACCXW010000000
VERSION     DACCXW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.079"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            50..292
                     /gene="yaaA"
                     /locus_tag="IVY71_001903"
     CDS             50..292
                     /gene="yaaA"
                     /locus_tag="IVY71_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7985952.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            279..1403
                     /gene="recF"
                     /locus_tag="IVY71_001904"
     CDS             279..1403
                     /gene="recF"
                     /locus_tag="IVY71_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7985953.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1400..3346
                     /gene="gyrB"
                     /locus_tag="IVY71_001905"
     CDS             1400..3346
                     /gene="gyrB"
                     /locus_tag="IVY71_001905"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7985954.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3366..5837
                     /gene="gyrA"
                     /locus_tag="IVY71_001906"
     CDS             3366..5837
                     /gene="gyrA"
                     /locus_tag="IVY71_001906"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7985955.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6073..6372
                     /locus_tag="IVY71_001907"
     CDS             6073..6372
                     /locus_tag="IVY71_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7985956.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6422..6934
                     /gene="ssb"
                     /locus_tag="IVY71_001908"
     CDS             6422..6934
                     /gene="ssb"
                     /locus_tag="IVY71_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7985957.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6960..7196
                     /gene="rpsR"
                     /locus_tag="IVY71_001909"
     CDS             6960..7196
                     /gene="rpsR"
                     /locus_tag="IVY71_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7985958.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7339..9312
                     /locus_tag="IVY71_001910"
     CDS             7339..9312
                     /locus_tag="IVY71_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP7985959.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9318..9770
                     /gene="rplI"
                     /locus_tag="IVY71_001911"
     CDS             9318..9770
                     /gene="rplI"
                     /locus_tag="IVY71_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7985960.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10044..11411
                     /gene="dnaB"
                     /locus_tag="IVY71_001912"
     CDS             10044..11411
                     /gene="dnaB"
                     /locus_tag="IVY71_001912"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7985961.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12191..13483
                     /locus_tag="IVY71_001913"
     CDS             12191..13483
                     /locus_tag="IVY71_001913"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7985962.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4552 a         2467 c         3007 g         3623 t
ORIGIN      
        1 aaaaggtata atagagtata cttttaataa tgaaaatgag ggatgggcat tgaaacaaca
       61 agtcattcta aatacggaat tcatgacgtt gggccaaatg ttgaaagaag tcacagttat
      121 cggtagtggc ggacaagcaa aatggtattt ggcagaaaac actgtgttag tcgatggcga
      181 accagaaaat cgtcgtggac gcaaacttta tcccggcatg atggttgaag ttccagaagt
      241 aggaactttt tttatggtaa aaaaaggaaa cgacgataat gaggctgaat aagctgtatt
      301 taaaaaatta tcgaaattac gaagagctga acatcgaatt ttccaagaac ctcgtcatct
      361 ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga aagtatctac gtattagcta
      421 tgacgcgaag tcatcggaca agtagtgagc aggaattgat tggctggact gacgatcagg
      481 cgatgattca aggagagatc acaaaaggtt catccactct tccattagaa atcttattat
      541 ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca gaaaaagtta agtacttacg
      601 tgggacaaat gaatgtcatt ttatttgcac cagaagatct ttctttagta aaaggcagcc
      661 cgcagatcag gcggaagttt ttagatatgg aattaggcca gatcaatcca atctatcttt
      721 atgatcttgt ccaataccaa gcaattttga aacaaagaaa ccagtacttg aagcaattga
      781 atgaaaaaaa acaaactgat ttgctttatt tggatatttt gtccgaacaa ctagcagcgt
      841 ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa gcgattggag ttttgggcta
      901 attctttgca tcagcaaatc acccatcaaa aagagcagct ggaaatcgag tatctcaccg
      961 cagtcgattc tttagaaaca catacgcaag agcaaatcca agaacaattt ctcgcacttt
     1021 tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac gacagttggt cctcatcgtg
     1081 acgatctttc cttttttatt aatcagaaaa atgtgcagac ttatggatcg caaggacagc
     1141 aaagaacaac tgcactaagt gtcaaattag cagaaatcga tttgattaaa gaagagacag
     1201 gggaatatcc aatccttttg ttagatgatg tgatgagcga gctggatgat aatcgtcaac
     1261 tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt cttaacaaca acgacacttg
     1321 accatgtaaa aaataaaatg actgttgaac ctgagatttt ttatgttcaa caagggaaac
     1381 taaaaggaga agaaactgaa tgacagaaga aagaagttta gtagaacgcg ctaaagagta
     1441 tgatgccagt cagattcaag tacttgaagg acttgaagcc gttcgtaaac gacctggcat
     1501 gtatatcggt tcgactagtt ctgagggtct gcatcacctt gtttgggaaa ttgtcgataa
     1561 ttcgatcgat gaagtattag caggttttgc aacgaaaatc catgtgatta ttgaaaaaga
     1621 caacagtatc accgttattg acgacggtcg ggggatacct gtagatatcc aggcaaaaac
     1681 tggacgtcct gcagttgaaa cggtctttac tgttttacat gctggaggaa aattcggcgg
     1741 tggtgggtac aaagtttctg gtggacttca cggagtagga tcttctgttg taaacgcgct
     1801 ttctacacag cttgatgtca aagtatacaa agaaggaaat gtgtactatc aagaatatcg
     1861 tcgtggagct gtagtggacg acttaaagat tatcgaacaa acggatcgcc atggaacaac
     1921 ggttcacttt acgccagacc ctgaaatttt tacagagaca actgagtttg atttttcaaa
     1981 attggctacc cggatccgtg aacttgcttt cttgaaccgc gggatgcgta tatcgattga
     2041 agacaaacga gaagaagaac cagtcatcaa tgaataccat tatgacggcg ggatcaaaag
     2101 ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt ccagaacctg tatatttgga
     2161 aggtgaacag caagatatcg cagttgaagt ctctatgcaa tatacagacg gctaccattc
     2221 caatatcatg agttttgcca acaatatcca tacgtatgaa ggtggaacac atgaatcagg
     2281 attcaagact gctttaactc gtgtgataaa tgattatgcc cgaaaacaaa agctgatgaa
     2341 agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa ggattgacag ctgttatttc
     2401 tatcaagcat cctgaccctc aattcgaagg acaaacaaaa acaaaattgg gcaattccga
     2461 agtacggaca gtcactgatc ggttatttag cgaacatttc atgaaattct tgctggaaaa
     2521 cccaagtgtg ggccggcaaa ttgttgaaaa aggattacta gcttccagag ctcgtctagc
     2581 agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct ttagagatta gtaatctgcc
     2641 aggaaaatta gcggactgct ctagcaacga tcctgaaaaa tgcgaattgt tcatcgtcga
     2701 aggagattcg gccggcggat cagctaagca aggtcgtaat cgagagtttc aagccatctt
     2761 gcctattcgt gggaaaatct tgaatgttga aaaagcaagc atggacaaga tcttggcaaa
     2821 tgaagagatc cggtctttat ttactgctat gggtacaggg tttggagaag attttgatgt
     2881 atcgaaagct cgctaccaca aattagttat catgactgat gccgatgtcg acggcgcgca
     2941 tatccgtaca ttgctgctga ctctttttta tcgttacatg cgtcctattg ttgaagctgg
     3001 atacgtctat attgctcagc cacctttata cggggtaaaa caagggaaga atattacgta
     3061 tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa gtcgttgctt cacttcctgc
     3121 ctcaccaaaa ccaagcgttc agcgttataa aggacttgga gaaatggatg atcatcaact
     3181 atgggaaaca actatggacc caagcaatcg gatgatggcc cgcgtcagtg tggacgatgc
     3241 tattgcagca gatcagatat ttgaaatgtt gatgggggat cgagtagaac cccgtcgtgc
     3301 attcatcgag gaaaatgcac actatgtgaa gaacttagat atttaatcat gaaggaggaa
     3361 aaagaatgag tgaagaaatc agagaaaaca tccatgatgt caatctaacc agcgaaatga
     3421 aagactcctt catcgattat gcgatgagtg ttattgtcgc acgtgcactt cctgatgtca
     3481 gagatggtct gaaacccgtt catcgccgta ttttatacgg catgaacgaa ttaggtgtaa
     3541 caccggataa agcccataaa aaatctgcca gaatcgtcgg agatgtcatg ggtaaatacc
     3601 atcctcatgg agacattgcg atctatgagt caatggtacg gatggcacag ccattcagct
     3661 accgttatat gctagtggat ggtcatggaa actttggttc tgttgacggt gacggtgccg
     3721 ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc tacagaaatg ctgcgtgata
     3781 tcaataagaa cactgttgat ttccaaagca actatgatga tacagaaaaa gaaccagttg
     3841 tactacctgc tcgcttcccg aatcttttag tgaacggaac aacggggatc gccgtaggta
     3901 tggccacgaa tatcccacct cataacttgt ctgaggtagt agcggctatc gatttgctga
     3961 tggataatcc tgatgtgacg accaatgaat tgatggaagt attacctgga ccagattttc
     4021 caacaggtgg tttggttatg gggaaatcag gaatccgaag agcgtatgaa acaggtaagg
     4081 gttctattac cgtacgtgca aaagttgaga ttactgagat gccaaatggt aaagaacgga
     4141 tcatcgtgac agaattgcct tatatggtca ataaagcaaa attgatcgaa cggatttctg
     4201 aactgcacag agacaagcgt atcgaaggga tcacagattt gcgggacgaa tcttcccgtg
     4261 aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag cgcatccgtt atcttgaata
     4321 acttatataa attgacatcc ttgcaaaatt catttggatt caatatgttg gcgatcgaaa
     4381 aaggtgtgcc gaaagttttg agcttgaaac aaatccttga aaactatatc gaacatcaac
     4441 gcgaagtcat cacacgacgg acggtctttg aaaaagaaaa agcagaagca agagcacaca
     4501 tcttagaagg tttgcgtatt gctttggatc atatcgatga aatcattgcg attatccgcg
     4561 ggtctaaatc agatgacgag gcaaaagcaa caatgatcga acggttcgat ttatccgatc
     4621 gccagtctca agctatttta gatatgcgtt tacgccgatt gaccggtttg gaacgggaaa
     4681 aaatcgaaaa tgaataccaa gaactcttaa aactgattga agatttaact gacattctgg
     4741 caagaccaga acgtgttaca gaaatcatca agaatgaatt agcagacttg aatcaacgat
     4801 ttggtgataa acgacgcaca gaattactag tcggtgaggt cttaagtctg gaagatgaag
     4861 atttaatcga agaagaagaa atcgttatta cattgacaaa caatggttac atcaaacgtt
     4921 tggcaaataa tgaattcaga gctcaacgaa ggggtggacg tggtgtccaa ggaatgggta
     4981 tccacaatga cgacttcgtc aaaaatctgg tttcttgttc tacccatgat acattgttat
     5041 tctttacgaa taacggaaaa gtgtatcggg caaaaggata cgaaattcca gaatacgggc
     5101 gtacagccaa aggaatccca atcatcaatt tactaggtat cgattcgagt gaaagcatcc
     5161 aggcaatcat tgcggtgacg ggagaagcag aagaaggcca ctatctcttc tttacgacgc
     5221 gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc taacatccga agtaatggtc
     5281 tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa tgtgctactg acagatggtc
     5341 aatctaatat cattattgga acacataacg gctactctgt cactttcaaa gaagaagcag
     5401 tccgcgatat gggacgtaca gcttccgggg tcagagggat acgcctgcgt gaggaagatt
     5461 atgtcgttgg ggcagcctta atgaaggaaa atcaagaagt attagtgatc acagaaaaag
     5521 gttacggaaa acggacaaaa gtatcggaat atccagtgaa aggccgcgga ggtaaaggga
     5581 tcaaaacggc gaacatcact gaaaaaaatg gtccactagc tggattgacc actgtttctg
     5641 gagatgaaga catcatgctg attacagata aaggtgtcat tatccgtttc aacgtatcga
     5701 ctgtttctca aactggacgt tcaacattag gcgtacgttt gatgaagatg gaagctgata
     5761 cgaaagttgt cacgatggca gcagtcgaac cagaagctgc agaacaaaca gttgaagaac
     5821 aaggatcaga agaataacca tcaagactaa gtgtgtaagc tagcaagaat aaccgattaa
     5881 aaaatctggt tattcctgct ggcttttttg attagttgaa tttctcttaa aatattcgaa
     5941 actgttcatt ctgtattgct tttgaccaaa atagagtgta taattagagc ttgtgagtag
     6001 tctaattaat tagataactc tccttgctct taggaaccta agagccgaaa gtccacaggg
     6061 aggtgaatat caatggaaaa cacgaaatat gaaatcatgt acattattcg tccaaacatt
     6121 gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg caatcttgaa agataacgga
     6181 gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt tcgcttatga aatgaacggt
     6241 taccgtgaag gtatctatca catcgttaaa gtatcttctc catcatcagc aaatgctgtc
     6301 aatgaatttg accgtcttgc taaaatcaac gacgatatca ttcgtcacat gattgttaaa
     6361 gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa cgatgagagg agatgactat
     6421 tttgattaat aatgttgtat tagttggacg tctgacgaaa gatccagatt tgcgttacac
     6481 agcaagtggt acggcagtcg caacatttac tttggctgtg aatcgtaatt ttacaaacca
     6541 aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt tggcgcaaat cagcagaaac
     6601 tttagcaaac tatgctcgta aaggaacact tttaggtgtt actggacgta tccaaactcg
     6661 ttcttatgat aatcagcaag gacaacgtgt atacgtgaca gaagtagtag ctgacaactt
     6721 ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa caaagcggcg gattccaaag
     6781 ttctggtcaa tcagctggcg gatttggcgg aaacaataac tctaatcaaa catctcaatc
     6841 atccaacggt atgcctgatt tcgaccgtga tacttctgac ccattcggct caagttcaac
     6901 gatcgacatt tcagacgatg atttaccatt ctaaacgaat ttgataggag ggaaatagaa
     6961 tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt agactacatt gctgctaacc
     7021 atattgaata tatcgattat aaagatatcg aattactaaa aagatttatt tcagaacgcg
     7081 gtaaaatctt gccacgtcgt gtaactggta caggcgctaa aaaccaacgt aaaattactg
     7141 ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt cgttagcgaa gactaataaa
     7201 tcctaggaat tgaatgactg atccttttga tcagctattc aattcctttt tttctgtaaa
     7261 aataacctct attcgtttag tcgctcgaat gacgaaagtt atggtaaaat caaattagtc
     7321 atatatggga ggaaaattat ggaaaataaa aacaatcatt tatccccgaa acctctcttc
     7381 cttatcctgt ttatattaat cgaagtattg gtagtcttaa ttttaccatt tcgttttgtt
     7441 agcgcttcgg tcattctatt gatgaattta tggggtgttc gctattggtt gaaaatgaaa
     7501 cgttggctta ttttaaataa acgtgaaaaa atccagcagg cttcgactgt agctgaaacc
     7561 aatctagctt atgtctcaga agtcatgcct gtaggagtga ttgcttatca gccatcaagc
     7621 caaaaaatcg aatggctcaa tccattagca ttgaaaatca aagatcaaag tggattaaca
     7681 gaggaagaat tgctagatac tttttttact gcaaaagaaa aaaagaaaaa ccaagtctat
     7741 ctagcagata aaacatttca gtttgatatg gatacagtaa agggtgttgt ttattttaat
     7801 gatattaccc aaaaccagtt gttaaaactc catcagacga acagtcagcc ggtgattggt
     7861 attttatcta ttgataacta tgcagatgcc attgataaat tagatgacaa agagatttct
     7921 tatttgaact ctttcgtcac tacactagtt tctgactgga tgaatgaata ccatgtcttt
     7981 ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac agatgatcga tctgaaaaag
     8041 atgatggata aaaaatttga tttgctggat cgctttcgag cagaagctga aaaacagagc
     8101 tcgcctatca cccttagtat ggggattgct tatggctcaa gaaccctcga tgaaatcggt
     8161 ggagaagctc agaataattt agatgttgct ttagtccgtg gaggcgacca agttgtcgtg
     8221 aaagaggtaa aagaaggagc aaaacctgtt tattatgggg ggaaatctgc taagaatgtc
     8281 aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt tgcgtcggat ttttgatgaa
     8341 acaggcgatg tgtttatcat ggggcacaag tttccggata tggatgcttt aggctctgct
     8401 tttggtgtca gttgtctggc acgcttcaac cagaaaaaag cttacatcgt tatcaatgag
     8461 aacgagatca ttccagatgt agaacggtgt cttgaagaaa tccataagca tcctgaatta
     8521 gaaatgcaac taatctctcc agaacgtgcc atagagctga aaaaagaaga ggacctcctc
     8581 gtgatggtgg attaccataa accttctcta tcgatctctc agggcctata tgagcaattt
     8641 gataagattg tgatcattga tcaccatcgt cgtggggaag agtttccaag taaacctctg
     8701 ctgacttata ttgaatcttc agcttcttca gcttcagagc tagtttctga gctgatccaa
     8761 taccaaagca gcaaaacaaa tcgcttagat cgttttgagg ccacattatt gcttgcaggg
     8821 atggttgtag atacaaatag tttcaatgtc cgtacaactg cacgaacttt tgacgttgcc
     8881 agctatttaa ggtcttgtgg agcagacgca tccttagtac aatatttatt gagctcggat
     8941 ttagcgacat acttggagat cagcaatttg attgctggaa gcgactatgt gacttctgat
     9001 attgttgtgg cggcagggac agacgaaaaa gagtataata gtgttatagc agcaaaaaca
     9061 gcagatacat tgctttcgat gatcaatatc aatgcagctt tcgtcattac aaagcggaca
     9121 gacggcttga ttgggatcag tgccagaagt accggcacga tcaacgtcca aatcatcatg
     9181 gaatccttag gtggcggagg acacttcaca aacgcagcta ctcaactgga aaatacgaca
     9241 gttgaggaaa caaaacagaa gctatttgct gcaatcaaag aaaatatgaa tcaaatatat
     9301 gataaggagt aatccaaatg aaagtaattt ttttacaaga tgtaaaagga aaaggtaaaa
     9361 aaggggacat caaagaagtt ccagcaggtt atgcacaaaa ttatctaatc aaaaataact
     9421 tggcaaaaga agcaacgaaa agcagtatca gtcagttagc aggacaaaag aaagcacaag
     9481 aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt aaaagaattc ttggaaaaag
     9541 aagatactgt agtcgaattg aaggcgaaag caggggaaga cagccgtttg tttggttcga
     9601 tcccttcaaa acaaattgct gaagggttaa acaaacaata caaaatcaaa ttagataaac
     9661 gaaaaatcga attagaaaat ccgatccgtt cactaggcta cacaaacgta cctgttaagc
     9721 ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt acaagaataa caaatcttga
     9781 tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa gacaaaccga aatttatctg
     9841 agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg atttcagatt gatttcggtt
     9901 tcttttttgg gaacagcaaa atagcataaa gaaaacgagt ataataaaag agaactattt
     9961 ttttattaca ctttcggtac gaatgatgac ttttctctca ttttcttgta aaatgaagca
    10021 attgaagaca aggagcgata cgtatgaatg aagtgtggca ggatcgaatc ccaccgcaaa
    10081 atattgaagc tgaacaagcg gttttaggct ctgtttttct tgacgcagaa acgatcattg
    10141 atgccatgga gtacatagag ccaaaagact tttatcgtcg tggccatcaa attatttttg
    10201 agacaatgat cgagttaaat gatcgtaatg aagcgattga cgtggttact gtcaaagacc
    10261 ggctggaaca agccaatttg ttagaagatg ccggtggttt aagttatctc tcagacttgg
    10321 cattagcagt tccgacagca gcaaatatcg tctactatgc gaaaatcgtt gaagaaaaat
    10381 cattgctgcg tacattgatc cagacagcaa caggaatcgt tacgaaagga ttcgagcaag
    10441 gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag tatcttggag gtttcggaaa
    10501 aaagaaatcg aagcggcttt ttatctattg ctgaagtact gaactccacg attgaaaata
    10561 tcgatcgact agctcaaaac aatgaagaaa tcacaggtct accaactggc tatcaaggtc
    10621 tggataaaat gacagcaggt ttccagcccg aagaactaat cattcttgct gcacgtccag
    10681 cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa tgtaggaaca aaaacagatc
    10741 gttctgttgc gatatttagt ttggaaatgg gggcagaatc tcttgtaaat cggatgcttt
    10801 gtgcagaagg ttcgattgag gccagccatt tacgtacagg tcagctttcg gaagaagaat
    10861 ggtcgaatct gatcgttgcg atggggagtc tttctcgagc aaatattttt atcgatgata
    10921 caccagggat caaaatctct gaaatccgtg cgaaatgtcg gaaactagcg caagaaaaag
    10981 gcaatctagg cttgatattg atcgattatt tgcagttgat tgaaggtaac ggtaaagaga
    11041 accgtcaaca agaagtatca gatatctctc gccaactgaa aaaactggcg aaagagctga
    11101 aagtacctgt cattgctctt tcacagttat ctcgtggagt agaacagcga caagacaaac
    11161 gaccagtgct aagtgatatt cgtgaatccg gatcaattga acaagatgcg gacatcgtag
    11221 ccttcttgta tcgagatgat tattatgaaa gagaaggcgg agatgacgat gatggaaacg
    11281 agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa gaaccgaagc ggtgcacgag
    11341 gaacagttga actactgttt atcaaagaat acaataagtt ttcgtcgctt tcgccaagag
    11401 aagaatttta aagaagaggt tgtgagcctt gagcaatccc gagagctctc gtaagaagat
    11461 gcaaggagta accgcaggaa cgactagagt acttaattta aacagatctc caaagggatt
    11521 gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac ggtgttcaat cagctgaccc
    11581 ttggtattag ctcagcaaac gtcaaaatat gaggaactat tttgacgttt gtttctctaa
    11641 tactcaggtc aaaacgctga tcttacgacc tcttttcctt aaacggtgtt ctcctgccaa
    11701 ctcctcggtc acaagtcaca atatcatcaa acatgagaag cagttttttg aaattgctcc
    11761 ttgtgactca gagttaaacg gcaggctcac aacctctttc ttaaacggtg ttcaaaagct
    11821 tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga gaagctattt ttgtttggct
    11881 gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg tgttccaaaa
    11941 gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt tgagaagcaa ttttcgtaca
    12001 ttccttctta tttcttgaag ctgaccactt ctgttacaac ctctttttta ataaccattc
    12061 taaaaagata aagttcgttt tttaagcgtt tttataaaat aaaaataaaa atcattcgat
    12121 tttatttgaa atatgtatct attattgata caatggttca ggattaaata atatagaacg
    12181 aggtgttcac atgtcatctg tagtagtagt aggtacgcaa tggggcgatg aagggaaagg
    12241 aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt gcacggtacc aaggtggcga
    12301 caatgcaggg cacacaatta agtttaacgg tgtgacttat aaactgcatt tgattccttc
    12361 tgggattttt tataaagaca agatcagtgt gatcggaaac ggcgttgtag tcaatcctaa
    12421 atcattggtt aaagaattag cttatttaca tgaaaaaggt gtagaaacaa gtaatttgcg
    12481 tatttctgac cgcgcgcacg taatcttgcc ttatcatatc aaattggacc aattacaaga
    12541 agatgctaag ggcgaaaata agattggtac aacgattaaa ggaatcggac ctgcctatat
    12601 ggataaagca gctcgtgtag gtatccgtat tgcagactta ttagataaag aaatttttga
    12661 agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa tttgtaaaaa tgtttgattc
    12721 agaaccactt gattttgacg atatttttga agaatattat gaatacggac aacaaatcaa
    12781 acaatacgtc acagatactt ccgttatctt gaatgatgct ttggatgcag gcaaacgcgt
    12841 gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac cagggaactt atccttttgt
    12901 tacttcttct aacccagtag ccggtggggt aacaatcgga agcggtgtag ggccttcaaa
    12961 aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca cgtgtcggtg atggaccatt
    13021 tccaactgaa ttatttgatg aaacaggaga acaaatccgc gaagtaggac gagaatacgg
    13081 tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat tctgtagtta tgcgccattc
    13141 taaacgagtt tcaggtatta ccaacctatc tttaaactcg atcgatgtat taagtggatt
    13201 ggaaacagta aaaatttgta cggcttatga attagatggc gaactaatct accactatcc
    13261 agcaagcttg aaagagttga accgctgcaa accaatctat gaagaattgc caggctggtc
    13321 agaagacatt acaggatgca aaacacttgc agaactgcca gaaaacgcac gtaactatgt
    13381 acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca ttttctgtcg gacctgatag
    13441 aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc taaggataaa aacgacgact
    13501 gaatcaggac gttcttacca gagcgagtcg aagtgtttat ctatacgata ataataactt
    13561 gaagaggtag tgaaagagac gtttagcctt attgccgata ggctgtctct tgaacactgt
    13621 ttattcagaa taagggactg tgacaaaaa
//
LOCUS       DACCXW010000079        13163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCXW010000079 DACCXW010000000
VERSION     DACCXW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.080"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..326)
                     /locus_tag="IVY71_001914"
     CDS             complement(<1..326)
                     /locus_tag="IVY71_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7985963.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDE"
     gene            complement(323..1015)
                     /locus_tag="IVY71_001915"
     CDS             complement(323..1015)
                     /locus_tag="IVY71_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985964.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(1021..1692)
                     /locus_tag="IVY71_001916"
     CDS             complement(1021..1692)
                     /locus_tag="IVY71_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7985965.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(1685..2026)
                     /locus_tag="IVY71_001917"
     CDS             complement(1685..2026)
                     /locus_tag="IVY71_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985966.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(2204..2902)
                     /locus_tag="IVY71_001918"
     CDS             complement(2204..2902)
                     /locus_tag="IVY71_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7985967.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2989..3459
                     /locus_tag="IVY71_001919"
     CDS             2989..3459
                     /locus_tag="IVY71_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985968.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(3502..3681)
                     /locus_tag="IVY71_001920"
     CDS             complement(3502..3681)
                     /locus_tag="IVY71_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985969.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3668..4006)
                     /locus_tag="IVY71_001921"
     CDS             complement(3668..4006)
                     /locus_tag="IVY71_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7985970.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(4022..4225)
                     /locus_tag="IVY71_001922"
     CDS             complement(4022..4225)
                     /locus_tag="IVY71_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7985971.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(4240..5016)
                     /locus_tag="IVY71_001923"
     CDS             complement(4240..5016)
                     /locus_tag="IVY71_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7985972.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            5315..5635
                     /locus_tag="IVY71_001924"
     CDS             5315..5635
                     /locus_tag="IVY71_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7985973.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5653..6084
                     /locus_tag="IVY71_001925"
     CDS             5653..6084
                     /locus_tag="IVY71_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7985974.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            6211..6795
                     /locus_tag="IVY71_001926"
     CDS             6211..6795
                     /locus_tag="IVY71_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP7985975.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            6897..8045
                     /locus_tag="IVY71_001927"
     CDS             6897..8045
                     /locus_tag="IVY71_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7985976.1"
                     /translation="MLKVWVEQTKDGRFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALENYNKSDITFDELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYSSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRLTDPDQYIFISTSGTPLTLHSFNAVLHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(8134..9759)
                     /gene="groL"
                     /locus_tag="IVY71_001928"
     CDS             complement(8134..9759)
                     /gene="groL"
                     /locus_tag="IVY71_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7985977.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(9810..10094)
                     /locus_tag="IVY71_001929"
     CDS             complement(9810..10094)
                     /locus_tag="IVY71_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7985978.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10319..10981
                     /locus_tag="IVY71_001930"
     CDS             10319..10981
                     /locus_tag="IVY71_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7985979.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(11057..12349)
                     /locus_tag="IVY71_001931"
     CDS             complement(11057..12349)
                     /locus_tag="IVY71_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7985980.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            12519..13148
                     /locus_tag="IVY71_001932"
     CDS             12519..13148
                     /locus_tag="IVY71_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP7985981.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
BASE COUNT         3996 a         2570 c         2197 g         4400 t
ORIGIN      
        1 ttttcgtcaa taggggtatt gcgtttttcg tcaatagggt attgattaat tcgtaggtac
       61 ctattgatta tttgattggt accctctttg taaatgattt cccgattcaa gtatccaaac
      121 ttaatcaaat cacttaccca tcgcgatatg gtctctttat tcacaccata taaatctgca
      181 aagtactcat tgcctgccca acaaaagcct ctttcattac acaaggccgt tatctctccg
      241 tataacaact tagtatttgg tttaagtctt ttgtcgtacc ttacgttggc tggtataatc
      301 gcataataac ttcgatgttc tgtcattttt cccctccaat atttaacttt ttgattgttt
      361 tctggtttaa tttgatccct ttgatttgat atttattttt gaaattaatc acacctattt
      421 tgtgcttctc tgtgtgatgg attctgcaga gtgctgcaaa tgtgtactct gcatgatcaa
      481 cttctttgcg ctttcgtctt cctagcgctt tgtcaaagtg atcgatgtca gctcctgttt
      541 tgccacagat gcagcagact ctttttgtga tgcatttgta gaagtaatat tcttgattcg
      601 caggtaaaat ctcatagcct tctttgaaag gaatatgatg ttcaaagatg aaatctaaga
      661 tgatatttgc taagacattg gcatcactca cagttgtatt cgattcgtct ttcaggctta
      721 ttttgcgccc tgtgacacct tcaaaacgaa agtagaagaa ttccttccag aagtccgttg
      781 gcatgcctgt atcgataaaa atatcgccta tcagcgcata gatgaagttt cgttgctgta
      841 cggtgaaacg tctaggatca ataaaacgaa tttcaatgac tcgatcacca tcgtagccgt
      901 catacatcgt ctttagtcga tcaatgttca cttcctcatt gatggttgcg cttatgtctt
      961 ttcctttgaa ctttttcaga accgctgaat atgaatcgat taatggttta aacactcata
     1021 tcacttctta tctaattctt ttctcttagc tgctattgct cgctccatca aggcacattg
     1081 ctcatagctt aactgttcaa tagtttcaac gttatcagct aagagcccta atttatctgt
     1141 ctgctcatta acatattcga ttaaggtttt ggtcatatct ttacccatct gctcattgaa
     1201 agcttctaga atcgtctcta gcatgtttaa tttctttgta tcgattctag gtggtgttgg
     1261 aatatcttcc ccttgaaata catataatcc cagtccgtgt agagccaatg ctttcacaaa
     1321 gcatcgcttc aatgagttat tgatttgcat tgcatttggt ttaacaactg gttggtttcg
     1381 ataatctaaa acaggaaata attcggtttc cgtgtgtcct ttaaccgtta ctgagacaga
     1441 tacataagtc ccagtttcat ccataagaaa aggtttatat tcctcaacaa gaaagtcttg
     1501 atgagttcca gaaacaaccc tgtagtgttt gtactcatta atagttaccg ttgcctgtgg
     1561 atcattcttt ttcataatct cccacgcgtg agcccaagat aaataatcaa aatttccttt
     1621 tttcttgaga attttattta acttacgact aaaaagtttt tcaaagttcg ttgtcccttt
     1681 gatttcactc atcaaattct gcctccattt cagcaatgta tttcttacct ggtccgtaat
     1741 aagagatatc aatcaagtta tctctgtcgt actcttctag cgcatcaatc aagccatctt
     1801 cgatgacgta aatatattca ggtttattcg aatgcttcga tagatggata agataaacat
     1861 gatcccaaat agttacatgg ttgcctaaat catcttgatc ccaagctagt tcttcattcg
     1921 tcaaaagatt tcgtctgatt tttcgaccac ttgtttcctc aattttcggc ttgccccaat
     1981 caggatcaat caaatattga tctagagtgg aaagttcttt ttccatatgc taaaatctcc
     2041 ttatgatgtg ttttctttgt gactctttgc ttgccggcgg agtcactttt ttatttgttg
     2101 ccatgctttt tgcttgtcaa tatgttgttg gcttaggatg attggtttat agtatttcca
     2161 ccagcaatta gcaattgccg tccctattct tagcgcttca gctctattca ttgtcatcac
     2221 caaaaagtct ctgttgtctg ttcagttgat caatttccat acggatcgca gtttctggca
     2281 accacatttc aataaatgaa acagcatcat cgaatctctt acgaggtaac tcgccatatc
     2341 ttgggattga aaaggtacgt ttaaattcag accaaaattt tgagaatact tttttgctga
     2401 tttcttcata agctcggctt tcttttcccc ctagaactcc cataactttc atatttcctt
     2461 tttgcttaat ttcaaactct tgttgtccgc taattcgcat agtatcttta agcatggaaa
     2521 catctttttt aacatctttc atttcttcta attgatagat catcatatct tcaattgttt
     2581 gaggaacagt attcttgcga ataacatctt ccatttcgtt gaatgcttca atgtattttt
     2641 gtttgaagta gatagctttc tttcctgtaa accccatagc cagcaagaaa aatccatctc
     2701 tactaatgaa gaaaactcgt cgatttctgc catatgaatc tggttcatta ccttctacaa
     2761 acatctgtcc aaaattggac acatcttttt ttagtgcatc aatatctctt aaaacatgtt
     2821 gatgtttttt ctcgaagctt tctgccactt gtaagctcgt agtcacagct tctttatttt
     2881 tcaaaattac taattcttgc attatttctt ctctcctttt tggtataatt tagttaaaaa
     2941 ctggtggtgt ttattttgaa tttttttcac ttaacaattt ttcaatggat gtcaataata
     3001 agttttatat tgagtacgtt tttaacaggg ataaaaataa aaggaatgcg ccctcaacta
     3061 gaagttgaac ttaatgcttc ttacttcgct gcagatatga tttatacaaa agtaatcatt
     3121 tctaactatt caacagaacc agctatgtta gttaatttag aactttcatc cgctaactta
     3181 aaccgcagat ggtcagctac tccatttaag aaattaattg ctaaaggagg atcagtcgat
     3241 gataatagaa tatactctga agctgtaccg ctaaatattc ctcctaaaag tgctatatct
     3301 tgttaccttg catttgaagt agggaaaatt aattttaata aactccttaa tcaccaaaca
     3361 aaaatgatat ttactctaaa tagaacccaa atccacaaaa ttgttgatat caaaaataca
     3421 aacttcccaa tagagaaatt agtgaaagaa ttaaactgag taatagtaat atacattcta
     3481 gtttctcttt tctattcatt tttagtcagc cccctcggtt ggctttttcg ctctgtactc
     3541 agcttcagct tcatcaagcc ccataaaaat ccaaaccatg taaacaatcg ttcctatcaa
     3601 cgcttgcctg cttccccaaa gtcctaaagc gtagatgatt agcggtgcgc tgaacactag
     3661 cgctctatta aattttccca tccgcttgcc tccttaagat ttccgaaaaa tttgtttcta
     3721 aaaattcaag tgttttactt cttaaaaata gatatgtgtc tcttccttca actggataat
     3781 agacaaatcc atttttgttt ttctcgatat cgataatatt tctatatctt gggttcttta
     3841 aaactctaga agtaaaccaa tcatattttc tgttaatccg ttctagcact tctggcaacg
     3901 tcatccatct accagtatca tcagcttttt ttaactcctc ataatccact tgggagataa
     3961 ttacatagcc ttctggaatt gggatttttg cttctagata ttgcattagc tgttcctccc
     4021 tttacaattc gtacatagtg ataatcgaat ctataattct gtttgcttct gcagaagtct
     4081 ttttaccgtt taaaattaaa gataagtagc ttttgctaat tccaaatctt tcagcaagca
     4141 tggtgtaagt taagaacttt gaactttcga catattcttt gattttttct ctatctcgtt
     4201 gagtgatttc tgcaatgtca gtcatactaa aactcctttc taaccaattt cctctaaatc
     4261 catttgaggg taatatcctt ctttttttag taattcgtag ataaatagac gccctttctg
     4321 tgtccatttg gtattcatta caattttagt gccaccatcg gctttcggga tctcagttgt
     4381 atgagatttt gtgtatcctt ggttcatgtg ttttttgcac aataaccatt ggttaccgac
     4441 ttttttctga atacctagtt tatgaagtaa tttattcatc tgttgtggag acatcccata
     4501 atctgctgca atctgactaa ttgttactga atctgtagaa gataatatgc tatctaaata
     4561 ggagatttta ggttcgtact ctgcaatctt ttgttctgcg attaatcttc cagttctttc
     4621 ttcttttagt tgagttgcta attgaatgat tgtatctgga ttaagcaaag cttcttctac
     4681 tttttctgga gttagataac ctccatgttt tctaattgct ggcaacactt cacttgttac
     4741 ccaacgttta aattttttgg cagagggaag ttttgatttt aagattaaac tgtacaaacc
     4801 agattcgttg atgattgtca tctctcttga ttgacctgag gtcgtgattc gcgaccccat
     4861 cttatcttcc aaatctacat gccgcgacaa agcatcttta gtgtttgaat aacccaaaac
     4921 actcgcaacg tctttgccta caaaatatgg ttcatcattt actaaaattg tccgaacttc
     4981 gttttgttcg aaattaaaaa tttgcggtgt gttcatattt ctcattcctt tcttttatat
     5041 tcgtaaacaa atttaacaac ttttttctaa attcaattga cagtatctga gtaatactct
     5101 atactaagag cataattaaa taagacataa aaacattgat tttaaagctt tcttggcggt
     5161 ctgcatttac atatcaatag tgttttttgt tgtcttttta gttgttaaat ctgtttacaa
     5221 gatatagtat agctcattac tcagaattag tcaaattatt tctgcgtatt tttctaaact
     5281 ttttttgtaa acatttagaa aggttgtttt atcgatgaat acctatgaaa taataaaaga
     5341 cttggcgaag aggaaaaaga tatctatacg gcagttagaa attaattttg gatattctaa
     5401 cggttatcta gcaaagtgga aaaataatac tcctaattca gatgaattaa gtcggattgc
     5461 tgactatttc gatgtctctg tagactacct tctaggtaga gaagaaagag aaacccctaa
     5521 acatgtggat ttatcagaag acgatactgt tttttctttt gatggaaaag aaatatctaa
     5581 ggagacaatg cgtaaagcga ttgcaattgc taaagcttta gaggaaaatg aatagttgga
     5641 gtgatggttt gtatgtattt gaaattgaaa gaaatgttga gcgaatacaa tttgaaagta
     5701 gtttatatgg agatgaaaga gcctggtttc tactatccaa aacccagaat catttttttg
     5761 aatgaaaatc tatacggtga aactgcagaa gcctttcatt tatctcatga acttgcacat
     5821 ttcagcgcct ctcattttga attttcagtt ttgtacgata cttctacaac ttttcactca
     5881 aaatttgaaa cagaagcaga taaaatagct attctaattt tattaaatat ctatattgag
     5941 aacgaactga ctgatgaatc tcagtttaat ctcgaaaaat ttatggagta ttattctatt
     6001 cagaataagc tcagatacac ttgttatgct gtctgccaat gctattttaa gaaaaaatat
     6061 tcttacgcaa ggcaatatgt atgaatacat caagaattat taaaaaaacc gatttcagca
     6121 aaacattaaa aaagcccgtg ctggcacacg gactcatacc tcatttctga gatcgcaaat
     6181 atattataac aagaagtgag gaatatttaa atggcaaaaa aagttatggg tcaagacgga
     6241 aaaatgtata aagttagtaa acctttttac aaaaaagtat ggttttgggt attagcagta
     6301 attttaatta taattattgg ttctgcttta aatggtggat cagatagtaa taaagcaagt
     6361 gataatggtg gcgaaaaagt aactaaatct tcaacctctg cttcatcttc taaagaagaa
     6421 aaaagcgata ccttctataa gattggtgac actgtaaaag ttggtgatgc tgaatataca
     6481 ctaaatagtg tagaactaac tgatgaaaga aatcaatttg aagaaaacca acctgcacaa
     6541 gtagtaaaaa ttacttatac cgtaaaaaat gatggcgatt cagatatccc tgtaggtaca
     6601 gatgtggaag tatatggacc agatgacaaa aaatcagaga catatgccaa cgaaaataca
     6661 atgggatctg ttgctccagg aaaacaaatg gatgtaactg ctcatttcac gttaaaccaa
     6721 aagggagaaa tagaaatcca cttctctcct ttagtatcat ttgaaaaagc agctattttt
     6781 aaagcaactg tataataaat aaaaaacacg ccccaccgtc cagagtaaga gcgtgctttt
     6841 agaaaacaaa cctatatagg tctatttgtt atgcctatta tagcaaatga taggagatgt
     6901 taaaagtgtg ggttgaacaa accaaagatg ggcgattcaa gtttattgaa agatatgtag
     6961 atccttatac agaaaaaaca cgaaaaaaat ctacaacact tacaagtaat tcaccacaag
     7021 cgtggaaaaa agctcagaaa attctagata aaaaaattaa agaagcactc gaaaattaca
     7081 ataaatcaga tatcactttt gatgagctgt ataaagaatg gtatgaatat tataagcagc
     7141 atgttaaacg tactagctat ctgaaggtgc caatgatgat gaaacatgtt tctaagcaca
     7201 taagcgatga tacaatcgtt agaaacatag atgagacact cattaataag ataattgaag
     7261 atatgtatac gtttggtgac ctctcactga actacacaaa acaaacaaaa acaactctat
     7321 ctgttatgct aaactatgca atcgatagaa aatacatcca aagaaaccct gctctagcag
     7381 ttaaaatcca ccctaaaaaa gtggaagaag aaaaaagaaa gctttctatg gataaaaaat
     7441 atctggagaa agaagaaatt gatcaaatac tgaaacagct atactccaat cctcgcagaa
     7501 aactacacgg cataatagct gaatttttat atttgactgg tttgagatat ggggaattgc
     7561 tagctttaca aatgaaggac tatgaggatg ggaaaatttc cattaacggt accttagatt
     7621 actcatctgt gaaaatggat aatgctataa aaacaactcc aaaaaatact tattcgcaac
     7681 gcgaagtaca attgcccaat cgtgcaaaag aattgattga aagtgtgata gctgacaaca
     7741 ttcttgcggg tagactcaca gatcccgatc aatatatatt tatatctaca agtggcactc
     7801 cgcttacgct gcactcattt aacgctgtac tccataaagt agaggaagaa ttggagttag
     7861 aaaaaagtct atcatcgcat atatttagac atagccatgt ttcactatta tctgaattgg
     7921 gcgtacctct taaagccatt atggaacgtg taggacattc tgatgcaaat acaactctgt
     7981 ctatctataa ccatgtaacc aaaagagcca aacaacaagt aattgataaa ctaaatagcc
     8041 tttgatattt ttgccccttc tttgcccctt ttacttaaaa taaaacaaaa aaagaagaaa
     8101 gaaccctgtt aaaatagggt tctttcttaa ttactacatc ataccgccca tcattgatgg
     8161 atccattgca ggagctgctg gcgctgctgg ttctggttta tctgcaacga ctgcttctgt
     8221 cgttaataac aaggcagaaa cggaagcggc attttgcaag gcagaacgtg ttactttagt
     8281 tgggtctacg atacctgctt caaccatgtt tacccattct ccagtagcag cattgaaacc
     8341 agtgcctagt tccacgttct tcaatttatc aacaatcact gatccttcat aaccagcgtt
     8401 ttcagcgatt tgacgaattg gttcttctaa agcacgaaca acgatcttga tacctgttgc
     8461 cacgtctcct tctgcttcta cagcagaaac tttgctgata acgttcacta gagctgtacc
     8521 accacctgag accattcctt cttctactgc ggcacgtgta gcattcaatg catcttcaat
     8581 gcgtaatttt aattctttca attctgtttc agtagctgca ccaactttaa caacagctac
     8641 accacctgct aatttagcta agcgttcttg taatttttcg cggtcaaaat cagaagttgt
     8701 ttcagcaatt tggtttttga tcaattgcac acgagcttcg attgcttctt tttctccaga
     8761 accttctacg atcgttgtat tgtctttatc tacaacaact ttagaagcat ttcctaagtt
     8821 ttcgattgtt acatctttca attctaaacc tagatcgtca gtaattactg taccacctgt
     8881 caaaatagcg atatcttcaa gcattgcttt acgacgatcg ccgaaaccag gagcttttac
     8941 agctactacg ttgaatgttc cacggatttt gttcaataca agagttggca atgcttcacc
     9001 atctacatcg tcagcaataa tcaacaatgg acgtgattgt tgcaagattt gttctaacaa
     9061 tggcagaata tcttggatgt tagagatttt tttgtctgtg atcaagatat atgggttttc
     9121 tagaacagct tccattttgt cgttatctgt taccatatat tgtgataagt aaccacgatc
     9181 aaactgcata ccttctacaa catctagttc tgtttcgatc ccttttgatt cttcgattgt
     9241 gatgacaccg tcgtttccaa ctttttccat tgcatcagca atcaaatgtc caactttatc
     9301 agaacccgaa gaaacagctg ctacttgtgc aatggcttct tttgaatcaa caacagttga
     9361 gatattatgc aattcttcga ctgctgcttt tgttgccaat tcgatcccgc gacgaatgcc
     9421 tagaggatta gcacctgcag ttacgttttt taaaccttcg cgaacgattg cttgtgtcag
     9481 aacagttgct gtcgttgtac cgtctcctgc aatatcattt gtttttgatg ctacttctga
     9541 tacaagtttt gcacccatgt tttcaaaatg atcttctaat tcgatttctt ttgcgatcgt
     9601 cacaccatca tttgtgatca atggagagcc gtaagatttt tctagtacga catttcggcc
     9661 tttaggtcct aatgttactt tcactgtatc tgctaattta tctaccccgc gtaacatagc
     9721 tgcgcgtgcg tcttctgcaa atttcaattc ttttgccatg attgattcac ctcattgatt
     9781 atttttgatt atttttttat cttccaaatt tactcaacaa tcgccatgat atcttttcct
     9841 gcgacgatca gatattcttt tccttcgtat tttacttctg taccggcgta tttttcgaac
     9901 ataacttggt cgcctgcttt tacagcggca ggaacttttt caccgttttc taacaaacgg
     9961 ccttcaccga ctgcaacaac tgtcccagtt tggggtttct ctttagcagc agaagctaaa
    10021 acgattcctc cgacagtttt ttcttcttct tttgcgactt cgataatgac gcgatcacct
    10081 aatggtttta acacgataaa tccctccgat aactcttttt ttaatctgtt agcactctta
    10141 accttcgagt gctaacctac atttttaata atactcattc tccctctttt ttgcaagctt
    10201 ttaaatcaaa gaaaagaaat tttattctga cagctgctta ttgaaattat gatataattg
    10261 aaatcaatcg atataagctt gccttgcatg cttgttatcc cttaaaagga ggaattacat
    10321 gtctttaaaa aaatatagct tcctcagcat tttttgctat ggtctaattt ttatctcacc
    10381 cttgatccta gcttcgatag gacttgtaaa atctacgtct gaattgatca cagcaactgc
    10441 agtcatgtat atactaggcg ccgttgtcct agctattttt tattttaagc aacgtgaacc
    10501 cttagcgatc gaatcagcgg taaaaaaagc ttctgctcca aagattgtta tttatggatt
    10561 ggttggaatc tttgtcgcat tgattctgca aagcatcgct gtcatgttag aatctgtatt
    10621 gtttggcaaa gcaacccctt ccgagaatac acaaaatatc atccagatga tcatggaagc
    10681 acccgctttt attttggcga caactgttgc tggacccatc atggaagagt tcgtattccg
    10741 ccgcagtata ctggggatca tcagtcgata ttcaaatttt tgggtcggag cagtgatcag
    10801 ctctttgctg tttgcgtttg ctcataatga tggtcatctg ctaatctact ttttcctagg
    10861 tttcttcttc agcctagagt acaaagcaac tggtcggatc tggacatcga tgatcacaca
    10921 cgtaggaatg aacacattag ttgttcttgt tcaattagct gtccaaaaag ggctagtata
    10981 aacatgacta taaatactcc tgtcatccta gattggtgat tttcccaatc taaagcgaca
    11041 ggagtatttt tgttttttag tcgttcatct tttctgctgc tccatcattt tttgtctgtc
    11101 tttccttttt gcagtttgca gaatctattt ttttcaaaaa tgggaaataa atgaaaaaac
    11161 taattgacaa cacgatacct tgtaaaatcg ctgcaggcac tccgcctact aagaaacccg
    11221 agaggatcgg cggtgttgtc caagggactt ggactgctgt aaataaagga acgaatccaa
    11281 aatataaagc ggaataggta atcaatcctg aaacgaccgg tgcgaaaata aaaggaacag
    11341 ccatcatcgg gttcatcacg atcggtgtgg aaaaaataat tgattcattg atgttgaaaa
    11401 aggctggtaa aagagcaaga tttcctaatt ctctgtattt cttcgatttc gcaaaaaccg
    11461 tcataaaaaa tacagcccct aacgtcaatc cagcacccgt aaccgtcatg aactgatcga
    11521 gaaattgctg agtaacgata tgtccgccat ttgccaccgt caactcttgt cctgaggcta
    11581 agatttctgc attcgctaat gaattcgctt gcaagacggg actaaccaac ccattgacca
    11641 tcgcagatcc atgaagacca aataaccata aaaacggaac aaaaaaacct aataaaagca
    11701 caccaaacaa cgtgtctatg aacgattgaa tgggaagctg gacatatgtc catactcctt
    11761 gaacaacagt cattctgaac aactgttgaa aaaccgctga accgataagc gtaaaagtga
    11821 ctataacaat gattggaatc aatgaagtaa aagactgcca aacattttca ggtatcttct
    11881 ctctttgctt ccaccctttc gcaagtctta ctaaacgtgc atagccccat ccgatgatga
    11941 gcccagttgt aactgctcct agtatccctt cagcacctgt ccagacagta tctatctgag
    12001 tatttagtgg ctgaataagc agtagataag cagacaaggc tgttatgccg actggtaagc
    12061 gatcgatgtc ttcttctttt gcataagcgt atgcaatggc aaaaacgatc caaaaggcta
    12121 tcacatcaaa tgtagcctgg tatacttgtg taaaatacat agaaaatcct gttttgtcta
    12181 tccactgagc gatgtaatcc acaggaaaat gagcaaataa gaaaaacagc gagccaacaa
    12241 taattattgg gatcgtgtaa gtcattccat tacgcaaagc agttaagggt ctaagattca
    12301 caacagatgt tatcattggt aaaaaggcac gatctaacca ctctttcatt tcttatctcc
    12361 ttcaacttct atttttatca atcagttttc ttaatttaac tatacccaat gatcacgaac
    12421 catgaaagga atgcgcactg agcttttcaa aaatcggatt ctttgacgaa tattcagcaa
    12481 ctgcttacaa tggacaggaa taaaaaagga ggttttttat gaaaattgca ctcattggag
    12541 ctactggcca tgcaggttca tttattttag acgaagcatt gtccagagat ttagacgtga
    12601 cggcaatcgt gcgtcatcca gaaaaattgc catcagatgt tccgtttata caaaaggatt
    12661 tatttgagtt gacaactact gatcttgcat cttttgatgt tgtcgttgat gctttcaacg
    12721 ctcccaaagg aaaagaagaa atgcatcaaa cttctttgtc ccatttgatt gagatgcttg
    12781 aaggaaccga tacacgctta attgtcgttg ggggtgcctc ttcgttattc ttagatacag
    12841 aaaagacgac tcgtatgatt gaccaagtac ctaaaaatgc acccttctat ccaacagctt
    12901 tcaatatgta cgaaggattg ttaaaattga aacaatctaa aaatctacat tggacttata
    12961 tcagtccagc ttctttattt gatccacatg gcaaacgtac aggtacttat ttgttaagtg
    13021 atgattattt acaaagaaac gctgctggcg acagtacgat cagcatggca gattacgcga
    13081 ttgcattagt agatgagata ctggatgaga aacatgaaaa gcagcacatc agtgtagtca
    13141 gtcgataata cggttatttt ttt
//
LOCUS       DACCXW010000080        12952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCXW010000080 DACCXW010000000
VERSION     DACCXW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.081"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            18..719
                     /locus_tag="IVY71_001933"
     CDS             18..719
                     /locus_tag="IVY71_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7985982.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            716..1837
                     /locus_tag="IVY71_001934"
     CDS             716..1837
                     /locus_tag="IVY71_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7985983.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1852..3084
                     /gene="pepT"
                     /locus_tag="IVY71_001935"
     CDS             1852..3084
                     /gene="pepT"
                     /locus_tag="IVY71_001935"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7985984.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3216..4124
                     /locus_tag="IVY71_001936"
     CDS             3216..4124
                     /locus_tag="IVY71_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7985985.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4159..4431
                     /locus_tag="IVY71_001937"
     CDS             4159..4431
                     /locus_tag="IVY71_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7985986.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4442..5488
                     /locus_tag="IVY71_001938"
     CDS             4442..5488
                     /locus_tag="IVY71_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7985987.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5739..8348
                     /gene="clpB"
                     /locus_tag="IVY71_001939"
     CDS             5739..8348
                     /gene="clpB"
                     /locus_tag="IVY71_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7985988.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8499..9170)
                     /locus_tag="IVY71_001940"
     CDS             complement(8499..9170)
                     /locus_tag="IVY71_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7985989.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9163..9657)
                     /locus_tag="IVY71_001941"
     CDS             complement(9163..9657)
                     /locus_tag="IVY71_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7985990.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9677..10897)
                     /locus_tag="IVY71_001942"
     CDS             complement(9677..10897)
                     /locus_tag="IVY71_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7985991.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11044..12879
                     /gene="lepA"
                     /locus_tag="IVY71_001943"
     CDS             11044..12879
                     /gene="lepA"
                     /locus_tag="IVY71_001943"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7985992.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         4245 a         2372 c         2723 g         3612 t
ORIGIN      
        1 ctagaaggag tgaagctatg aaccatttag atttatctca gcgcttagcc gcagtgggtg
       61 actttgtccc tgaatccgct cgtttagcag acattggatc agatcacgct tacttacccg
      121 ttgcattgat attaagagga aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc
      181 cttatgaatc agcacgtaag caagtcaaaa aagatggatt agaagaccgt atcaccgtac
      241 gccttgcaaa tggtctagat gctatccagc cacaagatgg tattacagct gtcacgattg
      301 cgggtatggg aggttcactg atccgagata tcctagaagc tggcaagttg aatcaacgct
      361 taagcggaca agaacgtttg atccttcagc caaacatcgg agagcgtaca ttacgatctt
      421 ggctgcaaga aaatcagtat aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa
      481 tatatgaaat cattgtagcc gaaaaagaac aaaaaccagt tacttacaat gaaatggagc
      541 tgatgttcgg gccatttctg cttgaagaga aaagtgagat cttcaaatca aaatggacaa
      601 gagagcttca acaaagaaaa gctatccgtg aacagctcaa acaagcagct aatcaagagg
      661 cacgtcaaaa acaaatcgag caggaaatcc aaatgattga ggaggtttta aagccatgac
      721 aatcagcgga cgcactttca tccaaaagtt tgaaagctat tgtcctcagt ggctagctga
      781 agaaggagac ccagtcggac tgcatatcgg aacattggat aagccaatcc aacgagtaat
      841 gatgacatta gacgttcgtc cagaagtggt cgaagaagca atcaagaaaa aaatcgattt
      901 gctgattgct aaacatcctc caatctttcg tcctgtaaaa cggctggtta ctgatcagcc
      961 acaagaaaaa atgtatgcag atttgttgaa acatgatatt gccgtctatg cagcacacac
     1021 gaacatggat attatttggg atggactgaa tgactggttt tgtgaacttc tcggtattga
     1081 agtggagagt tatttagtaa agactcatga gattcattat aaaaaactgg ccgtttatgt
     1141 acctgtggat catgcacaga aaatgcgtga agtattagct gcagctggtg ctggtacaca
     1201 aggtgattat accggtacaa gttttacctc gattggacac ggtcgattta ctccggaagc
     1261 tggtgcacaa ccagcgattg ggaaagtagg gaagacagaa caagttcaag aggcaaaagt
     1321 tgaggttatt ttgccggaaa ctatcgaaaa acaagtgatt caagccatgc ggtcagctca
     1381 tccttatgag gaacctgctt atgatctttt cgctatagat gaaccagtcg aaatgtttgg
     1441 gttaggacga gtaggtgaat tgccgcaaga aatttctata gaggcatttg tagaacaagt
     1501 aaaagaagcg ttccaactag atggactgag aatagtccag ccaaaaaatg caaaatcctc
     1561 tgtgaagcgc attgcgattt gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc
     1621 ccaaagagca gatgtctata tcacgggaga tatctattat catacggctc atgatatgca
     1681 aagcgctggg ttgattgcaa ttgatcccgg tcattatatt gaatcgttat gcaagcaacg
     1741 atttatcgag aaatttgaat cttggaaaca agaagaaaat tgggatatta actttttcgt
     1801 ttctgaaaca aatacaaacc catttcaatt taattaaaag gagagaatac tatgtacgaa
     1861 aatcttttgc cacgtttttt acgatatgtc aaaacagaaa cacgctccga tgcaacaagc
     1921 aaaacgactc catcgactca gacacaagtt gcttttgcgc aagaattgaa aaaagaatta
     1981 gaagagttag gcttagctga tgttttctat aatgaagata acggattcgt tatgggaaca
     2041 ttaccaagca atgttgatca tgaagtgcgt tccattggat ttatttccca catggataca
     2101 gcagatttta atgcagtagg agtcaatcca caaatcattg aaaactatga tggcgagtct
     2161 acgatcgtac tagatgcgga agaacgctat acattgaata caaaagattt tccaaatttg
     2221 aaaaattatg aaggccacac attgatcaca acagatggca gtacattact gggagcagat
     2281 gacaaatcag ggattgcgga aatcatgact gcaatggaga tcttgatcaa taatccttct
     2341 atccctcacg gtaccatcaa agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg
     2401 gataaattcg atgttgagca attcgatgta gattttgctt atacagtcga tggtggtcct
     2461 ttaggtgagt tgcagtatga aacgtttagt gctgctcaag caaatattac gatccaaggg
     2521 aaaaatgtac atccagggac ggcaaaagat acaatgatca atgctctaca gttagcaatc
     2581 gattttcata atcagcttcc agccgatgaa gttccagaaa aaacggaagg atacgaaggt
     2641 ttcttccatt tgatggcatt ggatgggaat cctgaagaag cttctatgag ttatatcatt
     2701 cgcgatcatg atcgtgaaaa attcgaagca cgcaaagcac aaatcacaca tatccaagaa
     2761 gagttgaatc agcgatttga tcaagaacga atcaaagtag atatgtatga tcaatattac
     2821 aacatgaaag aaatcattga aaaagatatg agcatcgtgg aattagcgaa acaagcaatg
     2881 atcgagcttg gtatctctcc agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa
     2941 atctcttaca tgggtattcc gacgccaaat ctatttgccg gaggagaaaa tatgcacgga
     3001 cgattcgaat ttgtttcttt acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt
     3061 gcagaattga acacaaagag ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc
     3121 ccaatgatgg aatgtcatct gtctaaggct aaagggcagg acaatggaat tggttgtccc
     3181 gccctctttt ttgaagagta aataggaggt aaagaatgga aaagctgcta gttttttata
     3241 acaaaagttc agggaaagat gagggtgaga aattagcacg ctggtttcaa gattatgtaa
     3301 aagaaaagca gccagaatta tatgtagaac tagctgaaac tggcccatcg attgatgatg
     3361 cggaattagt caagaaagca gaagccatag aagctgatac tttagtaatt ataggtggag
     3421 acggcacgat tcatcatatc gtccaagcgt tccaaaaaac attatctcaa taccatgtcg
     3481 gattacttcc aggtggaact gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa
     3541 aagaagaagc tgctgacgtg atcctggaag gcaatgtacg gaaaatagac tatggtcaag
     3601 tcaatgatga agtcattatc tctacactga caatcggaat cttagcagat acagcagttt
     3661 ggatcagtca agaagaaaaa cagcgttacg gctcgtggat ctttatcaag aagtttttta
     3721 agttattgct gaagaagaaa aaatatcatt tggatttaga aacagacgag gagcattgga
     3781 aaggaaaatc ccaactgttg acagtcacga tgtctaactc cgtgggcggt tttacaaatt
     3841 ttgacgactc agcgacccta gatgatggaa aatttcatgt aacgatcgta ccttccttag
     3901 atattttccg atttattttt tatttgccta gaatcatgag aggcaaattc tatacgatcc
     3961 ccggcattac ttatttcaca gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg
     4021 gcactcgaac ggatggagac acgacagatg atcttccagt tgaattatct gttgtatcaa
     4081 aaggattaac agtttattca ccaaagaaag aagaaaaagc ttagaaatag tagttaacag
     4141 aaagttggaa gaagagtaat ggatgtattg ttacacaatc ctttgtggtt ttaccttttc
     4201 attgtgaatt tttacttatt ttgtctgatg gggtatgata aacatcaggc tagaactagc
     4261 ggctggcgtg ttcctgagaa taatttgata tttgtcagcc tgttaggcgg tggtttggga
     4321 ggattgatct ctcgaaaagt tttccatcat aaaactcgga aaaagaagtt tactatagga
     4381 ttcgctgcag gtgtacttgt tgatttgatt ctcatttatt ttttccatta aggagcagga
     4441 gatgtcgtct aaaggaacga aaactaaaat cattctgaat ctgcttttgc tgattgtgat
     4501 ttttgccatt atttattatt taatcaatca atcatttgca gatatctttg ccgaattgct
     4561 gtctaccagt gttgttgttt tggcagcggc tgttatatta gggacagttt accaaatcgc
     4621 tgaagggcgc tcaatcaaag aaattgctga acctttcgcg ccgaatttta caacgaaaga
     4681 tgggttcttg acctctagct atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac
     4741 gctgatctcg gagatttatt tttataacaa aaaaggaatg aagctatccc agggagcagg
     4801 ggtgacagca ctccatatga ttatgtacaa actggcagtg atcacttatg cattagctgg
     4861 cttggtcatc caattctctc ttttttattc taatggtcca tcaatgatct ggtttatcat
     4921 cgctggtatc cttttgacga tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt
     4981 gaatgttcaa atattttttg tcagcttatc gaataagtta tttaaaagtg agcgtttgcg
     5041 gaaatgggtg gatacatgca atcagcagat ctactcttta agggaaacgg tccaaaccat
     5101 tatccaagac aggactgcgc tattgcggat ttatctatgg aatatgttaa aacttgtatt
     5161 ttggtatgta cttccttatt tgattctgat tgaaaaccat cctaaaatag attttctact
     5221 gaccatttcg tttatcagct ttgcagtagt gctttcaggg gttattccta cgcctgctgg
     5281 tattggttcc tttgaatttg tctatttgtt attattcaga ccagttgttg gaacagttga
     5341 cgcagtttct tcgcttttac tgtatcgttt cagttccttt atcttgccat ttctctatgg
     5401 atttgtgtat gtgattgctg aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa
     5461 aaaagagaat aaagaaaatc aagggtaggt cttttataag atctaccctt tttatcaaat
     5521 caaagaaaaa cgttatggat aagataattt tacgtttgtt tggctctttt tttatataca
     5581 tacaatcaat tttgtttttt taagatttat ataagtcgag aaaaaaagtc aaaaaactat
     5641 gtgtatattt tgaccaaaat tgaccaaagc gatataatag tttttgtaaa gatcaaagaa
     5701 ataagtgatc ttacctttaa ttaggagggt atacctgtat gaatattgag aaaatgacta
     5761 cgacgcttca agaagcgatt gctgaagctc aacagattgc ggtcactcga aaacatcaag
     5821 atatcgacat tgcccatgta tggaaaatct ttttacaatc taatcatttt ggtcgaaatt
     5881 tctatacgga tgccggattg gatgtcgaat cttttgaaca tgaaattgat cgtctactgg
     5941 atgagtatcc agttgtttct ggtggaaacg tccaatatgg acaaaacctc agccagaatt
     6001 tctttcgatt attgaatgaa gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt
     6061 caacggaagt cgttatttta gcgttgatga aattaaaaaa ttatccgtta actgtttatt
     6121 tgaataaaaa cggattatct gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag
     6181 gagaaagagt gacctcacaa aaccaagaag aacaatacaa agcattagaa aaatacggcg
     6241 ttgatttagt acagcaagtc aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag
     6301 aaatccgtga tgtcatccgt attctttcac gaaaaacaaa aaataatcct gttctgatag
     6361 gagaacccgg tgtagggaaa acggcaatcg ttgaagggct tgctcaacgt atcgtacgca
     6421 aagatgtgcc tgaaaattta aaagataaaa cgattttttc attagatatg ggtgcattga
     6481 tcgctggagc aaaattccgt ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag
     6541 tcaaaaaaag tgatggacgt atcctcttgt tcatcgatga aatccacaat atcgtagggg
     6601 caggaaaaac agaaggaagt atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg
     6661 gtgaattaca tttgatcggt gccacaactt tagatgagta ccgtcaatat atggaaaaag
     6721 acaaagcgtt ggaacgtcgc tttcaaaagg tattagtcaa agaaccaaca gtggaagata
     6781 cgatcagtat tctgcgtgga ttaaaagaac gttttgagat ccaccatggt gttaacatcc
     6841 atgacaacgc attagtagca gcagctactt tatctgaccg ttatattact gatcggttct
     6901 taccggacaa agcaatcgat ttgatcgatg aagccagtgc aacgattcgg gtagaaatga
     6961 actctatgcc aacggaatta gatcaagtta ctagacgttt gatgcagttg gaaatcgaag
     7021 aagctgcact gaaaaaagaa tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag
     7081 aagaattagc tgaacttcgt gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa
     7141 aagaagaagt caattctgtt tcagcaaaac gtgctgaaat cgataaagca aaacacgaat
     7201 tagaagatgc agaaaataac tatgatttag aacgcgctgc tgtattgcgt catggtacga
     7261 tacctcaatt agaaaaagaa ttgaaggaat tagaagctaa agcaaaagac agcgagatta
     7321 agatggttca agagtccgtt accgaaaatg agattgctca agtcgttggc agattaacgg
     7381 gtatccccgt aacgaaactt gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa
     7441 cacttcataa acgagtgatt ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa
     7501 ttcgttcacg ggctggattg caagacccaa atcgtcctct aggttctttc cttttcctag
     7561 gaccaactgg tgtcggaaaa accgaattag cgaaagcgtt agctgagaac ttgtttgact
     7621 cagaagatca tatggtacgg atcgatatga gtgaatatat ggaaaaacat gctgtgtctc
     7681 gtctagtcgg agctcctcca ggctatgtag gttacgaaga aggtggtcaa ttaacggaag
     7741 ctgtcagacg aaatccttat acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg
     7801 atgtcttcaa tatcttgtta caagtcctag atgacggacg cttgactgat tcaaaagggc
     7861 gagtagtaga tttcaaaaat acggttctga tcatgaccag caatatcggt tcacagttac
     7921 tgctggaagg cgtgactgct gatggaacga ttccagaagc agtggctgaa caagtcaaca
     7981 cgctattacg tggaaacttc aaaccagaat tcttgaaccg aatcgacgac acgattttat
     8041 ttacaccatt gagcctagac aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg
     8101 cacaacgtct ggaacatcaa gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga
     8161 ttgctgaaaa tgcttatgaa ccagcatatg gtgcaagacc attgaaacga tttatcacta
     8221 aggaagtaga aacaccacta gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa
     8281 aagtaacgat tactttactt gatggacaac ttcatttcaa aacagaagag ctagaagaaa
     8341 tcgtctaatc ttccattgag actataaaag aaaaaagggg tctgacacaa ctagtgtcag
     8401 acctcctttt ttcaaataaa cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa
     8461 tttcttgaag ctgcccgctt ctgtcacaac ctcttttatt aggtattttt cactttttct
     8521 aatttataac tttcaaagta acgctcatct tttttacttt gagaccatgc aaacaatccc
     8581 attaaggcag cacctatcgt atttgtcagc aagtcgccca tcgtatccat caatgctgct
     8641 cgtccgacaa acagtgtgcc atcagaagca gcaaaacgtt gaagattcat gcctaagaac
     8701 tggtcgcata agaattccca gaactcccaa aacacgccgc aaagtccagc aaacgcaaag
     8761 ccgaataaga ggaacaacca aggactggtt tttgatatct cagcatcttt catcaagtaa
     8821 ccaatcaatc catagccaag agctgtcagg accattggac tcaccgcatg taagattttg
     8881 tcccaaaaag agatgatact gatcaaatgc atccctgtgc ctaaaaagac agagatgaac
     8941 aagaaaaacc agtaaaacaa aacgattgct gctggtattt taagtttgaa aactttcaaa
     9001 atgacttgtg gcatgaaaat caatgcaatc cctaataaag attccacgat aaatgtgatt
     9061 ccttgagaag tcgaatattt ttgtgtgata aattcataaa aattatagca gacaacgaat
     9121 acaccaaaag caagtaaagc tatttgcacc tttttgattt ctttaatcac tgaaagtccc
     9181 tccatctaaa aatcttttta cttctgaaag caactcaatt tctgaagaaa ccacttttcc
     9241 gttcaatttg atcaaaccta ttgtatataa gttcaggtag tgaaattgat tttcagctgt
     9301 ttcttgtagt gcagcgattt ttttcgggtg ggttccgcct tgctgtcgag tatcagtata
     9361 taagccgatc actgggatgc ctttcgcata agccacaccg atttccgatg ctaccccggc
     9421 atcgatcgtc agcccatcta gtaaagcaat cattaaatca ctagccaaca ctttttctgt
     9481 gtcagcaaga gcaatcattc gactgtctgc ataagcagtt ttgtcattga ttgcttcatt
     9541 ttcttgagga agatagatcg aaagctcaga agagagctct ctgatttctt taactaactg
     9601 ttggttatat aacaaatcac ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta
     9661 aacatcatcc tttttatcaa aaataatcgc taaagtaagc ttccatgttt ggaatgatac
     9721 gctctagtga cttcagtgtt tcttcatctg tttctaaccg ttcgattctt gccaaataag
     9781 aagcacggcg ataattcatc atcaagcaag tcaatgtttg gatatccagt cgaacggatt
     9841 ttcctatagg ttcatttaag acacggactt gtccctgttc atcccatgtt aaagcgaaga
     9901 caccattatt ccaaggagcg acaggatctt caatgatgaa atgaaaagca tcagctgttc
     9961 caacaaacgg aaaccgttga aggaattctt ttacatcaac gatccgagcc ataaaatatg
    10021 gttcgatctg ctctttgatc tgactatctt caatcaaaaa tgctaaaggc tcatttttat
    10081 aaatatctcc atgtacccag tagatcatgg aaaaatgtgc ggaaataaag ttccataagc
    10141 catttcgagc ttcttgatca agatagaaca tttcttttac gtgaaatatt tcttcagcta
    10201 cccagtagaa cagaacacct cttggttcat ggttgctatc gtagtagaca gcggctgtgc
    10261 gctcatcttc attttcgaaa cgccaatact cttcccaatg aaaatcactg cggaataaag
    10321 caccatggtt ttgttgggca aatttgtcat aaacggaaaa tacgtctgga tgatcaactg
    10381 gtaatcgctc aaccatacca cttactggga tttgtttagg tagctgtgta tctctgattt
    10441 taaaggaaag tttatcagac atgatttccc atcctttacg ccgatagtag gggatactgt
    10501 aaggatacaa ataagagatc cattgcttat tcttgcgcat attatccaat gcaacgataa
    10561 ttagatcttg catcaatcca tggttagcat attctggata agttccaact ccagtcacac
    10621 cacccatttt ataacgtgca ccgtgaatat tgacttcaca aggatagata gcgatttgag
    10681 agatcagttt attttcgtgg aaccatccga acactttcga tacttccaaa atcggttttt
    10741 tggaacggat aaaagctcgt ttattctcaa acccgctttc ctcgatgtct gcttcagtca
    10801 cttggaacac ataggaaagc aattcattga attgatcgat atgttcttga gtgaccggtt
    10861 ttaattcaag attttctcta aaatcttttt gatccatttt ttcatctcca tttattagct
    10921 tgataacagt atagcaaaat tctacaaaaa aaacttctat agctgactaa ttaaaaactc
    10981 tttaaattac tgcttttcgt tggtataatg gaaagtgctg attatatgaa aagaggagac
    11041 gctatggata taaagaaaat gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc
    11101 gctcatatcg accatgggaa atctacattg gccgatcgta ttttagaaat gacgcacact
    11161 gtgacgtctc gcgaaatgca agatcagctg ttagactcga tggatttgga acgtgaacgt
    11221 ggaatcacga ttaaattaaa tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca
    11281 tatatctttc acttgattga caccccaggg cacgtcgact tcacctatga agtctctcga
    11341 agccttgctg cctgtgaggg tgccgtttta gtggtcgatg cagcacaagg aatcgaagca
    11401 caaacattgg ccaatgttta tttagcgtta gacaatgatt tggagatcct gccagtcatc
    11461 aataaaatcg acctacccgc agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta
    11521 atcggaatcg atgctagtga agctgtatta gccagtgcca aatcagggat tgggattgaa
    11581 gatattctcg agcagatcgt ggaatatgtt cctgcaccaa gtggagactt agatgcaccg
    11641 ctaaaagctt tgatctttga ctctatttat gatagttacc gcggtgttgt tctaaatgtc
    11701 cggatcacgg acggtatggt caaacccgga gacaaaatca aactgatgag taatggcaaa
    11761 acatttgatg ttacagaggt cggggtcttc tcgccaaaag cagttgctag agactttttg
    11821 atggtcggtg atgtcggtta tatcacagca agtatcaaaa ctgtccaaga tacacgagta
    11881 ggggatacag taacgttagc agacaatcct gccgctgaag ctttaccagg ttaccgaaaa
    11941 atgaatccga tggtttattg tggtctttat ccaattgata cttctcgtta caacgatttg
    12001 cgggaagctt tagaaaaact gcaattgaat gatgctgctt tacaattcga accagaaacc
    12061 tctcaagcat taggttttgg gttccgctgt ggattcctcg gacttcttca tatggatgtc
    12121 gtccaagaac gcttggaacg ggaattcaat ctagaattga tcacgactgc accatccgtt
    12181 atttaccatg ttaataaaac ggatggtacg actgctacag tagataaccc ggcagatttt
    12241 cctgaacctg taacgattca ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc
    12301 ccaaatgatt ttgtcggtgc agtaatggaa ctttctcaac gaaaacgggg agaattcatt
    12361 acgatggatt atttagatga ttaccgagta aatgtcgtct ataatattcc gctttctgaa
    12421 attgtctttg actttttcga caaattgaaa tcaagcacga aaggctatgc ctcattggat
    12481 tatgaaatgt ctggttatca aaaaagcaag ttagtgaaga tggacatctt gctgaatgga
    12541 gaaaaagtcg atgcattaag ctttatcgtt caccgagact ttgcctatga acgtggaaaa
    12601 gcaatcgttg aaaaactgaa aaaactaatc ccacgccaac aatttgaggt gccaatccaa
    12661 gcagctattg gacaaaaaat cgttgcccgt tcagacatca aagccttgcg taaaaacgtc
    12721 ttggctaaat gttatggtgg agatgtttct cgtaaacgta aactcttaga gaaacaaaaa
    12781 gaagggaaga agcggatgaa acaaatcggc tccgtcgaag ttccgcaaga agccttcatg
    12841 gccgttctga aaatggacga ggacgaaccg aagaaatagc aagggtttgt ttgaaaacag
    12901 tgtaaaaaat aaaccatgtt tacatggatt tgccaccggt ttgccaccgg tg
//
LOCUS       DACCXW010000081        12864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCXW010000081 DACCXW010000000
VERSION     DACCXW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.082"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            107..1393
                     /gene="efmA"
                     /locus_tag="IVY71_001944"
     CDS             107..1393
                     /gene="efmA"
                     /locus_tag="IVY71_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP7985993.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1531..2130)
                     /locus_tag="IVY71_001945"
     CDS             complement(1531..2130)
                     /locus_tag="IVY71_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7985994.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2534..3655
                     /gene="mnmA"
                     /locus_tag="IVY71_001946"
     CDS             2534..3655
                     /gene="mnmA"
                     /locus_tag="IVY71_001946"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7985995.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3901..5322
                     /gene="arcD"
                     /locus_tag="IVY71_001947"
     CDS             3901..5322
                     /gene="arcD"
                     /locus_tag="IVY71_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7985996.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5336..6454
                     /locus_tag="IVY71_001948"
     CDS             5336..6454
                     /locus_tag="IVY71_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7985997.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6514..7806)
                     /locus_tag="IVY71_001949"
     CDS             complement(6514..7806)
                     /locus_tag="IVY71_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7985998.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7956..8516
                     /locus_tag="IVY71_001950"
     CDS             7956..8516
                     /locus_tag="IVY71_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7985999.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8645..9022
                     /locus_tag="IVY71_001951"
     CDS             8645..9022
                     /locus_tag="IVY71_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7986000.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8955..11027
                     /locus_tag="IVY71_001952"
     CDS             8955..11027
                     /locus_tag="IVY71_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7986001.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11238..12185)
                     /locus_tag="IVY71_001953"
     CDS             complement(11238..12185)
                     /locus_tag="IVY71_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7986002.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12431..>12864
                     /locus_tag="IVY71_001954"
     CDS             12431..>12864
                     /locus_tag="IVY71_001954"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7986003.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         4145 a         2338 c         2745 g         3636 t
ORIGIN      
        1 tgaaacgatt gttatttagg gaaaaaggta ttttttgaaa aaagaatccc gtataatgat
       61 tgttgtgcaa acgctataca aaatgaaaaa gaaaagaggg atgtttatgg aaaatgaaca
      121 gtcggtcgtt ttaacgaatt ggaagagaaa ttatttgttc tttttatcgg gacagttttt
      181 gtcaggaatc actagtatgg tcgtccaata tgcgatcatc tggtatttga caagagaaac
      241 cggttcggcg acaattttga gttttgctac tttactagga atgattccca tggttttact
      301 aagcccattt gtaggaccgc tagtggatcg ctgggataaa aaagctcttt tgattgtcac
      361 ggatattatt gtagcgattt tcgcgcttat tttagcagta gtcggaacga tttcagaatc
      421 cttcccgatt tggctagttt ttgtgtcttt gtttatgcgt tcggtcgcac aaacattcca
      481 aatgccaacg atccagtcga ttatgccgac aatcgttcca tcttctcaca taacaaggac
      541 gaatggacag ttaggaatgg tccaatcagc gaatttcatc attgcgccag ctctaggtgc
      601 ggctttgttt tcagtcgttc ctgtcaatta ccttatttta ttagatgtat tgggggcagt
      661 ttttggtgtc ggcttattga tttttgtgaa gatcccaaaa gtttctcctg aaatattgga
      721 agttccgctt actattttta aagatgcaaa atttgggctg cagcaattga tggataataa
      781 agggttatgg tacataacga tcaatggtgc atttgtgatg ctgttgttca tgccagcaat
      841 cagtctgtat ccattgatga cgctagatta ctttggtggt tccgttggac aggcgggagc
      901 agtggaagta gtctacgcag tcggaatgct cttaggcggt gcactgatca gttttatagg
      961 aacatggaaa gaccgaatga aacctattat tatagcctac atcattatgg gcctgacgat
     1021 cggtgcaagc ggattggttc caaacgatag tcaagggttc ttgtactttc ttatcttgaa
     1081 tgcaggagca ggttgtgcaa cgccatattt caatacactg ctgatggcaa tgatccagca
     1141 gagttatgaa tcgaatgttt taggtcgtgt cttagggaac ttcaactctt tgatgaacct
     1201 ggcaggtcca attggattat tatttgcagg accattagct gatcgtttag gggtagaaaa
     1261 aatgttcttg ttttccggaa tcgggatttt attatgcgga atcgttttat ttttaacgtc
     1321 agctgcccga aattatgata aagaattaca gaaaaaactg gtaaaagaac accatgagca
     1381 aaaagacgaa tagtatggaa tggagttgtg acagaagtct tgttacaact ccatttctga
     1441 tcctaatgtt cctgtggcaa taagcctact caaatgaaaa atatgagtaa ttatctttat
     1501 ttgtttaata cttgatatag ggcagttttt tcacgaccta tcgtacttcc tctttgcttc
     1561 atagatttct ttgatttgtt gctcagataa cggagtgcca aatggtagtt tttcctttaa
     1621 gagttctctt tccggtgcgt ctacgcctac attgacatcg ttctcgatag gtacgctgta
     1681 atgatggata tgtccatgaa gattgcggat agtgtcgtta ggcccgagca acatcggata
     1741 atgagtcaag taatattgtt gatggtcgaa tttaacgatg gcacctacat cataaaaata
     1801 aaatttggga cttccgtcag taagcctagg atcatttttt tctaaataac ggaaaaaagc
     1861 gcgactgtca tggttccctt tgatcaagtg gattgttcct tgcagttgag tgaccagttc
     1921 caaaatttct gcatttgctt tttcatattg cggatgcata gcaaggtcgc ctagatgata
     1981 aacatggtcg gtttctttta ttactgcatt ccagctagtg atcaactgtt ggttcatttc
     2041 atccacattt gcaaagagcc gcggagcaaa gtcgtttttt cctaataagc gttcatggaa
     2101 aaagtgcata tcactgataa agtaattcat gtatcttttg cctccctttt ttcttagagt
     2161 aaacaaaatc acttatctga gcaactcata acatctaaaa agaaaaggtt gtaaatgaga
     2221 cgtgcagctt taagcgataa gaagcaattt tgaaaaatag tttttcacaa tttataaaat
     2281 tattccttat tgccgaaagg ctggcccttt ttatgagtat gccaattttt tatatcgaat
     2341 gctcaaaaga tgagtgattt tttggttgac tatcatgttt gtgtactgaa taggtccagt
     2401 agaacagtat ttttacgact tgataattaa gggatgaaat tttcagaaat atgacgtata
     2461 atgtaaaata ctgaaaagtt gcgaccttca actcgtagat gtttcagaat ctaattgaaa
     2521 tgatggtgat atcatgacag acaacagcaa aacacgcgtt gtcgtaggga tgagcggcgg
     2581 tgtcgactca tctgtaacag cacttttgtt aaaggaacaa ggatacgacg tgatcggaat
     2641 attcatgaaa aattgggatg acacggacga aaacggtgta tgcacggcca cggaagatta
     2701 taaggatgta gcgaaagtag ctgctcaaat cgggattcct tattactctg tgaattttga
     2761 aaaagaatat tgggatcgtg tatttgaata tttcctagcc gaataccgcg caggacgtac
     2821 acctaaccca gacgtgatgt gcaacaaaga aatcaaattc aaagcattct tggattatgc
     2881 aatggatctt ggcgccgatt atgtagctac tggacattat gcgcaagtca ctcgcgatga
     2941 aaacggcgtg gtccacatgc ttcgtggagt agacaataat aaagatcaaa cttacttcct
     3001 tagccaatta tcacaagaac agctttcaaa aacgatgttc cctctaggcg gcatggaaaa
     3061 atcagaagta cgggcaattg ctgaacgcgc aggccttgcg actgctaaga aaaaagattc
     3121 aacgggaatc tgctttattg gtgaaaagaa cttcaaacaa tttttgagca actatctgcc
     3181 agcaaaaaaa ggaaatatgg tcactcttga tggtgaagtc aaaggacaac atgctggctt
     3241 gatgtattat acgattggtc aacgtcaagg actagggatc ggtggcggag gagattcgca
     3301 agaaccctgg tttgttgtag gaaaagatct agcgacaaac acattatatg tggggcaagg
     3361 gttccatcat cctgcattgt atgccacaag cttggatgca agtgaaatcc actttacgac
     3421 gaatgaacca atgccaaaag aattcaagtg tacagcgaaa ttccgttatc gtcagcaaga
     3481 cgttcctgtg acagttcgct tattggatga taatcgtgca gaagtcgtat tcgacgaacc
     3541 tgtacgtgca atcactcctg gacaagcagt tgtattttat gatggaatgg aatgtctggg
     3601 tggtggtttg atcgaccacg catatcaaga aacaaaagtt ttacaatacg tttaagcgat
     3661 attaaatgaa tcaaaacagc tatggctaac aaaagaaaca ccttttcttt tggatagcat
     3721 agctgttttt ttgcaataaa attaattttt attcatttta tacttgaaat acttgtgtaa
     3781 aaagttcata atatccaata atatgaataa agttgctttt tgttaagcaa gagcagttgc
     3841 aaaatagaag ggaatctttt gcaagtgatt tgttcaattt tataagtgag ggtgatggaa
     3901 atggaaaaga aatcgacggg tattagcaaa tggggactgg ttgctctggt tgtcagttcg
     3961 tcaatcggaa gcggtgtgtt tgggatcaca agtgatctgg ctagttctgc agctccaggg
     4021 ccagcgatcc tttcatggat aatcgttggg attggtattt tagctttggt attatcgctg
     4081 aatcatttag gagaaaagcg tccagatcta gatggtggta ttttcggtta cgcggaagct
     4141 tcatttggta aattaggtgg cttcatcagt ggttggggtt actggttgtc tgcgtggctg
     4201 ggaaatgttg cttttgcaac gatgctgatg agcgccatgg gagaattttt cccgatattc
     4261 aaaggtggac aaaatgttcc ttctatttta tttgcaagta tttttatttg gggtctgact
     4321 ttattagtaa acaatggagt tgaaggagcg agcgtcatca atacaatcgt gacgatctgt
     4381 aaattagtgc cattattcct atttttaata tttgtattta tcgcatttaa agtgaatata
     4441 ttcactgctg atttttgggg taacgtatct gataatctag tgaatggaac agggcagcaa
     4501 ggaacgatct ggttgcagat taaaggttgt ttgatggtta tgatgtgggt ctttgtaggg
     4561 atcgaaggcg caagtgtttt agccaaccgc gcgaagaaac gttcagaagc ccagcaagca
     4621 tcgatcattg gtctgctttg tttattgttt atctatatcc ttgcttcgat cttgccatac
     4681 ggcgtcttat ctcaagaaga gttagcaact atttcacaac ctgctatggc aaatatttta
     4741 aaaggaatcg tgggaaattg gggagcagcg ctgatcaata tcggcttgat tatttcgatc
     4801 attggttctt ggttgtcttg gacgatgctc ccagcagaaa caacgatgtt gatggctcga
     4861 gacggtctgt tgccgaaagg ttggggaaaa acgaataaaa aaaatgcccc aacttattcg
     4921 ttagttgtta ctgcaatctt gactaatcta ttcttattga ctttcttagt tactgattat
     4981 gcctatcaat ttgcctattc tttatgtaca gcggcaattt tgatttgtta tctacttgtc
     5041 gggatctatc aagtccagtt ttctttaaaa gaaggaatca aaaaacaagt agtgatcggt
     5101 gggattgctg tagtttttga attgatgggc atcattttag caggattttc ttatgtcctt
     5161 ttatgtagta ttgcctatct tcctggtttt tgcttctact ggatcgcttg tcgtgaaacc
     5221 aaacatcaaa ttacgggaaa agaaaaagta atgattgggc tgatttgtgt ggccgcactg
     5281 ctttcaatcg ggttgcttgc tgtaggctgg atcaagatat aagggggaaa gaaacatgaa
     5341 aatcgcaaat ttcggtgtag aagaatggct aaataaatgg gaaaagaaag cggtatatga
     5401 tattgcacaa agctcgatcg aagcattgac attagacgaa ctcgttggct tagatgggac
     5461 tagcgtaagt aaatttttcg aaaaacgcaa aaacgaacca ttggactatg gctggatcga
     5521 aggatcagat gttttcaaac aagaagtcgc ttctctttat caaaccgtcg atccggaaaa
     5581 catcttacaa acgaatggag cgactggtgc taatctttta gctctatacg cgttagtgga
     5641 agcaggagac catgttgttt cgatgcttcc tacttatcag caattatatg acatccccaa
     5701 atctttaggt gccgctgttg actttgttca tttgaaggaa gaagaagatt ggcagtttga
     5761 tttagaacag ctagaagcct tagtcaaacc tgaaacaaaa atcatctgtt tgaatagtgc
     5821 aaacaatcct acaggcacat tgctagatcg gaaaacttta gaaaaaatcg cagaaattgc
     5881 tcgtacagta gatgcatatg tattgataga tgaagtatat gcgcctttaa cggataaagg
     5941 tgaattcttg tcgatcgtag atgtctatga aaaaggtata gcaaccaatt ccctatccaa
     6001 aacctactcc attccaggta ttcgtattgg ctggacagct actggacctg agttggcaga
     6061 agtttttcga aagtatcgag actacacgat gatttgcggc ggagtcctgt cagatgatct
     6121 tgctgtacat gcattgaaga ataaagaaaa aatattggcg cgaaacaaaa agatcattac
     6181 agagaacctt gtgattttaa aacaatgggt agccaatgaa ccgaaagttg aacttgtcgc
     6241 tcccaattac gtctctactt ctttcattaa attagccatc gaagaagatg accagacatt
     6301 ttgtatcaat ttattagaag aaacaggagt actattagta ccaggaagtg cctttgattt
     6361 accgaagcat gcccgactag gttactgttg taaaaaagaa accttggaaa agggattatt
     6421 cctcttatct gagttcttga aaaaacaggc ctaaacaaaa acactgcaag gacagtctgt
     6481 gacaactgtt caagcagtgt ttttcattta cgcttattgt ctatgagaag gagagtgccc
     6541 tgaagctatg gagactcctg aagctttttc ttctgcagtt tcttgctgat ggatctcttg
     6601 tgtattgtgt cgtacaagca agaagttgac caaaacaaga agagaagtcg aaagaaagac
     6661 gccgcgataa ccaaatgcac tggaaacact ggagccgatc ataggcccta ctacgttacc
     6721 agttgcttgg aaagattggt tataactgaa gatccgtcca gcagcttgat gaggcgaata
     6781 ttttgtgatc aatgcttgga cagcagggag cagacaagca tcagagatac caatcaaaaa
     6841 acgtaaggca gctaattgcc aaacattcgt gacgaatgcc attgggatat aaaccagaat
     6901 cgcaaaaccc aaaccaatcg caagtattct ttcactccca atcttgtcac ctaagcgacc
     6961 aaatcttgga gcggctatca aagtggcaat cccaggtatc gaagcaatga ttccgctgat
     7021 caacgtaaca ttcccatgtc catgcagcaa ttggcgaatg tacagactga tgattggact
     7081 aatggaattg ttggatgctt ggatgatcat cgttgtgacg aacataccga tgacgacgtg
     7141 tggatatttc aagtctttga aaatctgttt ggcagaaacc atgttttctt ttttcactgg
     7201 gacaaatttt tcatggacga aaaacagact caacaagaag acgaaaaata agatgatccc
     7261 tgtaatgaaa aaggtagggc gatagccaaa cgctgaagca gagatcccgc caagaagcgg
     7321 tccaagtaat gttcctgtaa cagaaccagt tgctaaggtt ccaagaactt gaccactttt
     7381 ttcttttggc gtcccagttg cgacaagggc agtggcatta ctgatataac cagaaaaaat
     7441 cccttgaagc agacgcaatg cgactaattg atacacacta gtcacgcaac ccatcaaaga
     7501 aatgacgatt gccattccta atgaagcacg caacagcatc aattttcttc ctttttggtc
     7561 ggctaatttt ccccaccaag gagagataag cgtagtcacc aagaaagtag atgaaaaagt
     7621 caatccgctc caaaagttca attgtgacgt actataatta cctaacgtat ctatgtaaag
     7681 tgacataaaa ggcatgacta aactaaatcc gataccagcc ataaatgttc caaaccataa
     7741 gacaaacaag ttttgttccc aagtttctct tttcctaaat aatttttcct ttattcttgc
     7801 agccaattag actccttctt tctgaaaatt ttcatctaca gctctttatt ataaactaca
     7861 atatgtagtt tatcaatgaa agtctctttt ttctctttct ttttcgtggt aaactgaaag
     7921 agtgaaggat ttattttatg agaggaaaca tggaaatgaa aaaaagaaat ctgtctcaag
     7981 agaaaatcat cgactgcttt cgagaactag cagaagagat ggatgttcag caaataacct
     8041 ttcagcattt agccaaacgc ttggatatca aggctccctc gctgtataac catttcaaaa
     8101 atattcgtga tgtcaaaaca gcgctaacag cgaaacttct gcaggaactt aatgaccagc
     8161 tgcgacgggc tcttgtggga aaaagtggtc aggaagcact gcagatctat gcagaaactt
     8221 atcgatcctt tgctttttca aatcaagcag tgtatgagtt actgatcagt gtaccgcata
     8281 ccaatgaagc tgttttatta gatggaatcc atgaaacgaa tcagatcatt ttgcagatat
     8341 ttgaggcttt tcaattaacg aaagaagaaa aagttcatcg aagcagggaa cttcgtagta
     8401 tgatccacgg ttatctttcc ttacgttttt taggatactt tacaaaagaa ccaacaatcg
     8461 atccagaaga cagctatttt tggatgatca ataactttat cgctactttg ccttaaacaa
     8521 tcagattcat ttgtaacaat tttaagatgt tcattaaatg acccttttat aagagaaaat
     8581 tcttgcaact ttcttgaaaa gtgactaaga tgagtctaga aaagctgaaa gaaggagcac
     8641 gagtatgtat caagtaatta ccatgttcgg ggacaatgag ccttggtggt tttttgaaga
     8701 ctgggaagaa gatattgaag aagaagaaac attcgagtct ttcgaagaag cacgacaagc
     8761 gtacgaaaaa caatggaacg aaatccatca aaattatgaa tatatcaatg caaaaagtaa
     8821 ctttctcagc gccttttgga atgacggaga tgagcgctgg tgtgaagagt gtgacgatga
     8881 tttacagcaa tacaagggac ttgcgttgtt gaaaaatcat cagcccatca cagtagaaag
     8941 cagaaaggaa ttttatgaaa caactaattc tagcggaaaa accaagcgtt gcgaaagacc
     9001 taagcaaggt gcttggtgct aatcaaaagc acaaaaatta ttatgaagga cccaaggtga
     9061 tcgttacttg ggcccttggt catttattag gattgaaaat gccggaagat ctgaataagg
     9121 aatggcagag ctggcaaatg gacacgttgc caatgatccc taaaaaacta ggaatcaaac
     9181 cacttcctaa aacaggacat caattaaaag caatcaagca gctggctcag cgaaaagatg
     9241 tctcagaagc agtcattgcg acagatgccg gaagagaagg ggaacttgtt gctcgctgga
     9301 tcttagagta tgtgcatttc aataaaccag tgaagcggtt atggatctct tctcaaacag
     9361 ataaagcaat caaaactgga ttcaagcaac taagacctgc gaaagaatat gatgcattgt
     9421 atgaatctgc tcttgcccgg gcaaaagcgg actggctggt aggattgaat gtgaccagag
     9481 ccttaactgt gaagtacgcg gataatttat cagcaggccg tgtgcagaca ccgacactag
     9541 cattagtgag agaccaagaa aagaaaatcg agcagttccg tccacaaacg tatttcacga
     9601 tcacgttgca agctggaaaa gaacaagcca aaatgcttca aaaaaatcaa tttgctttaa
     9661 aaacacaaga agaagcacaa aaacttgttc aattgatcag tacaagcaaa ggaactgtca
     9721 cagaagtcca agaaaaatta aaaacggaat ctgcgccatt gccttatgat ttaacagaga
     9781 tccaaagaga agccaatcaa cgctatggct attctgcaaa gaaaacatta agtcttgtac
     9841 aaagtttgta tgagacccat aagatcgtta cttatccgcg tacagacagc aaatatctga
     9901 caaatgacat gaaaagtacg atgaaagaac gattgcaagc agtcagcgac tttgctcctg
     9961 aggtcaagca gtatttgaaa aacggagcca tcgtccaaca gacaaaagta ttccaagaca
    10021 gtaaagtgac cgatcaccat gcacttttgc caactgaaaa cagagcacgt tatgaaaaat
    10081 taagtaatga agagcgaaaa atctatcaaa tgattgtctc tcgtttttta ggactgtttg
    10141 ctcaacccca taaagtcagc caaacaaaag ttacggttga atttggtaaa gaacgattca
    10201 tttttcggca aaatcgtgtc gtccaagcag gatggaaagg cgaaactgaa actgagacgg
    10261 aaacagtcaa atgggaaaaa ggaatgagaa tcaatcccga tttcgccatc aaaaaagaac
    10321 tatctgcacc gcctaaacca ttgtcagaag caagcttgct tggtcaaatg gaaaaataca
    10381 gtctgggaac cccagctacg cgtgcagaga ttatcgagaa actaatcaaa tctgaactga
    10441 tggaacgtac ccctcaagga ctgcaagtat ctcctaaagg taagcaatta ttagtattgg
    10501 taaatccctc tttagtaaca ccagatttga cagaaagctg ggaaaaagac cttgaagcta
    10561 ttgctgcagg aaagaaacaa gcaggaacct tcttgaatgg aatcgaaaaa gaaacaaaac
    10621 gcttggtgaa tgagatcaaa tcaagcaaac aagaatatca agatttctcg attacccaaa
    10681 agaaatgccc agaatgtggg gccaatctgc gcgagaaaaa cacacgtgac ggcaaaatct
    10741 atgtctgcac caattcagat tgcaactacc gcagaagaaa agatccaaaa gtctctaatc
    10801 atcgatgccc gcaatgtcac cggaaaatgg aaatcatcga aggtaaaaac ggtgcttatt
    10861 tccgttgtaa atacgatgga acgacagaaa aattgcttga taaaaaagag cagaagaaga
    10921 aaatgaccaa acatgaagaa cgacgcttgc tgaaaaaata tgcacaagca gaagaaccag
    10981 aagaaagccc attagcagca gcactaaaag cagcaatgaa gaagtaataa gatatgaaag
    11041 aggttgtgat agaagtggtc agcttcaaga aataagaagg aatttacgaa aattgcttct
    11101 caaatttttg tgaaatttcg gcttatttcc gaagaagcta cttctggaac accatttatt
    11161 cggaaaaaag gactgtgaca agacttttgt cacagtcctt tttcatttaa aaaaatgttt
    11221 acttcatttg acgtatatta ttggtgaggc ttcaattcat ttggcttcac tttttctgtt
    11281 tctcttatga tataaacagg acgctgtttc gtttctaaga agattttccc aatatatttc
    11341 ccgatgattc caagacataa caattggatt ccaccaatga acaggaagat cgagaccatg
    11401 gaaggccatc ctgatgtagg atcaccaaac aacaatgccc gcaaaataat gaacaaaatt
    11461 gcaataccag aaccgacaca agacaaagcg ccgataaatg aagcaatcgt taacggtgca
    11521 tccgagaagt tcacgatacc atcaatcgag tagttgaata atttccagaa ggaccaagat
    11581 gtttcgccag ccacacgttc gcggttttca taagaaatat attctgtttt aaatccgacc
    11641 cagctaaata atccttttga aaatcggttg tattctgtta attctaggac ggcatccacc
    11701 atttgacgag tcatcaagcg gaaatcacga gcgccatcga ccatttctgt atcgctgatt
    11761 ttattgatca actggtagaa ttttttcgca aagaaactgc gtatcgcagg ctcgccttca
    11821 cgagtcgaac gccgggtgcc gacacaatcg atatcagaag tagtgatccg gttgatcatc
    11881 tcaggaagca gttcgggcgg atcttgcaga tcaacatcca tcacggtcac taaatctcct
    11941 gtagaagctt gcaatccagc gtacagagcc gcttctttcc caaaatttct ggaaaatgag
    12001 acaaagcgga cataaggata agctgaggct agcttcttga ttgctgctaa cgtaccgtct
    12061 tttgatccgt cgtcaataaa caaatattcg aactcatggg cacctttgaa tttttcaacc
    12121 tcatcaaaaa aaagaggaac agttttttct tcattaaaac atgggacaac aattgatact
    12181 ttcatcgttt ctatcctttc aatcgtatgt ttatatatgg tttacattca aaatttaata
    12241 aaccttaaat aacaccttta aaatttgatt ttacgtaaaa gaacaggtat tctttttgta
    12301 aactaacgta tttagtttac catgtttcct cggtttatca aacttttaag aacaattttt
    12361 attagttggc aagtagtttt ggatagagaa aaaggaggaa gttccagtct attgactagg
    12421 agcaaacaaa atgaaaatac acacacctat cggcttttta tggttagatg cagatgaatc
    12481 cggattgact gctgtatctt ttcagccaat ttcagaaagc gaatcagcga atcagcgaat
    12541 tttaacgcgc acacaagaac agtttgaaga atattttgcg ggaaaaagaa aagaatttga
    12601 tgttccttta tctataaaga gaggtacgcc ttttcagcaa aatgtctgga aagctttgtc
    12661 gaccattcct tatggagaaa tccgaacgta taaagaagta gccgttgctg ttgatagtcc
    12721 gaaagctgtg cgagcgattg ggcaggcaaa tcgaaccaat ccattgccaa tcgttattcc
    12781 ttgtcatcga gtgatcgggc aaaatggaaa actgacagga tatatgggaa acgcaataga
    12841 tggggttgaa atcaaacgcc aatt
//
LOCUS       DACCXW010000082        12781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCXW010000082 DACCXW010000000
VERSION     DACCXW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.083"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(287..649)
                     /locus_tag="IVY71_001955"
     CDS             complement(287..649)
                     /locus_tag="IVY71_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986004.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IVY71_001956"
     CDS             complement(723..1103)
                     /locus_tag="IVY71_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7986005.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IVY71_001957"
     CDS             1185..2360
                     /locus_tag="IVY71_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7986006.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IVY71_001958"
     CDS             complement(2437..3021)
                     /locus_tag="IVY71_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7986007.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IVY71_001959"
     CDS             3222..4220
                     /locus_tag="IVY71_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7986008.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IVY71_001960"
     CDS             4332..4841
                     /locus_tag="IVY71_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7986009.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IVY71_001961"
     CDS             complement(4871..5530)
                     /locus_tag="IVY71_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7986010.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IVY71_001962"
     CDS             complement(5532..6296)
                     /locus_tag="IVY71_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7986011.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IVY71_001963"
     CDS             complement(6304..6981)
                     /locus_tag="IVY71_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7986012.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IVY71_001964"
     CDS             complement(7018..8403)
                     /locus_tag="IVY71_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7986013.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8372..8626)
                     /locus_tag="IVY71_001965"
     CDS             complement(8372..8626)
                     /locus_tag="IVY71_001965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986014.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IVY71_001966"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVY71_001966"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVY71_001967"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVY71_001967"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7986015.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="IVY71_001968"
     CDS             9341..10198
                     /locus_tag="IVY71_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7986016.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="IVY71_001969"
     CDS             complement(10279..10614)
                     /locus_tag="IVY71_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986017.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="IVY71_001970"
     CDS             complement(10681..12051)
                     /locus_tag="IVY71_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7986018.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="IVY71_001971"
     CDS             complement(12129..12665)
                     /locus_tag="IVY71_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7986019.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3813 a         2588 c         2121 g         4259 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga caactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 ctttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatca
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg ctactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa
    12721 aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc
    12781 g
//
LOCUS       DACCXW010000083        12732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCXW010000083 DACCXW010000000
VERSION     DACCXW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.084"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVY71_001972"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVY71_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7986020.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IVY71_001973"
     CDS             complement(322..891)
                     /locus_tag="IVY71_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7986021.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IVY71_001974"
     CDS             1077..1979
                     /locus_tag="IVY71_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7986022.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVY71_001975"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVY71_001975"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7986023.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVY71_001976"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVY71_001976"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7986024.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVY71_001977"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVY71_001977"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7986025.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IVY71_001978"
     CDS             8547..8732
                     /locus_tag="IVY71_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7986026.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IVY71_001979"
     CDS             complement(8794..9990)
                     /locus_tag="IVY71_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7986027.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IVY71_001980"
     CDS             complement(10160..11596)
                     /locus_tag="IVY71_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7986028.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IVY71_001981"
     CDS             11889..12698
                     /locus_tag="IVY71_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7986029.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4009 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt ga
//
LOCUS       DACCXW010000084        12495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCXW010000084 DACCXW010000000
VERSION     DACCXW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.085"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..1957)
                     /locus_tag="IVY71_001982"
     CDS             complement(26..1957)
                     /locus_tag="IVY71_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7986030.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="IVY71_001983"
     CDS             complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="IVY71_001983"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7986031.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2886..3638)
                     /locus_tag="IVY71_001984"
     CDS             complement(2886..3638)
                     /locus_tag="IVY71_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7986032.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3797..4006)
                     /locus_tag="IVY71_001985"
     CDS             complement(3797..4006)
                     /locus_tag="IVY71_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986033.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4017..5344)
                     /locus_tag="IVY71_001986"
                     /pseudo
     CDS             complement(4017..5344)
                     /locus_tag="IVY71_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5709..6296)
                     /locus_tag="IVY71_001987"
     CDS             complement(5709..6296)
                     /locus_tag="IVY71_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7986034.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="IVY71_001988"
     CDS             complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="IVY71_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7986035.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7876..9165)
                     /locus_tag="IVY71_001989"
     CDS             complement(7876..9165)
                     /locus_tag="IVY71_001989"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7986036.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9319..10251)
                     /locus_tag="IVY71_001990"
     CDS             complement(9319..10251)
                     /locus_tag="IVY71_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986037.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10408..11067)
                     /locus_tag="IVY71_001991"
     CDS             complement(10408..11067)
                     /locus_tag="IVY71_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7986038.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="IVY71_001992"
     CDS             complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="IVY71_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7986039.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12108..12398)
                     /locus_tag="IVY71_001993"
     CDS             complement(12108..12398)
                     /locus_tag="IVY71_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986040.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
BASE COUNT         3471 a         2701 c         2099 g         4224 t
ORIGIN      
        1 aaacccagcc tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata
       61 aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg
      121 ggtgactcag taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga
      181 aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa
      241 tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga
      301 tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag
      361 caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac
      421 ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt
      481 tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag
      541 cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag
      601 ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga
      661 ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata
      721 cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg
      781 tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg
      841 aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt
      901 tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc
      961 caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag
     1021 gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat
     1081 ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac
     1141 cactaagggc tagattgatc aactcttctg gtttacggat accatcactg acaggacgat
     1201 tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta
     1261 caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt
     1321 tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg
     1381 ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt
     1441 ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt
     1501 tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta
     1561 attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat
     1621 gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt
     1681 ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa
     1741 ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg
     1801 cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa
     1861 acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga
     1921 tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc
     1981 aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc
     2041 atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc
     2101 tttcgtacgg ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga
     2161 atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa
     2221 agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt
     2281 cccatacgga atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc
     2341 gtgattgttt ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc
     2401 tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata
     2461 aaatcctttt cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc
     2521 tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa
     2581 gccattgatc tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt
     2641 tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc
     2701 agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg
     2761 ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc
     2821 gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt
     2881 gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct
     2941 aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac
     3001 aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct
     3061 aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca
     3121 ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta
     3181 gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc
     3241 tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt
     3301 ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct
     3361 tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg
     3421 agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca
     3481 tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat
     3541 tcagaagcat tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc
     3601 aagtctagga ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa
     3661 ataatagcat attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt
     3721 ataaatcagt caaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt
     3781 ctatcatcaa gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag
     3841 aagcaggttt tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga
     3901 ccaaaaaacc gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata
     3961 ataatgatgc cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat
     4021 attttttgta aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga
     4081 tgctgctcac ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg
     4141 aaggtaacat cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct
     4201 gaaggaacat agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct
     4261 acttctagat attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca
     4321 gtcggatcga taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa
     4381 tacgcctgag catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata
     4441 tcataacttt ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca
     4501 ttctgatact ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt
     4561 ttcaataaac cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa
     4621 taggattgaa acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg
     4681 atacatatga cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa
     4741 atcggtttag ggatgatttg atgataatag cgactataat acatttccca acacgaagtg
     4801 aaacataaac cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact
     4861 tccccgtttt gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat
     4921 agccgttgag agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag
     4981 gcgaacaccc catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt
     5041 tctttgaaaa cagagataga caatacattt tttatcaata attgattatc ttccaagcga
     5101 aaactacatt cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct
     5161 agcattattt tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag
     5221 cggataaatg ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt
     5281 ttttttcttc ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa
     5341 tcattttctc acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa
     5401 actaaaactt tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag
     5461 aaacaaagag gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact
     5521 tttcgaatca tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg
     5581 atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa
     5641 gctcaaaaaa agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt
     5701 tattcttttt atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt
     5761 tttgtaacgg aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg
     5821 atttcgctct catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca
     5881 acaattacag ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa
     5941 ggcttatgac gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa
     6001 tatgtctcga tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca
     6061 tatcccggaa catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg
     6121 gtctttcctg gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata
     6181 aaagaagatt tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct
     6241 ggatattgtt ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc
     6301 tctctcctta acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt
     6361 tacggcagcc tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg
     6421 ctcaccagac aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga
     6481 agggatatca aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc
     6541 accggtattg cgttcgattg cttttttcgc aatggctctc aacgcttcag gttcgaattc
     6601 aagttcggta ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg
     6661 ctcagtcaag atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg
     6721 taatcgtccg ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg
     6781 ctgcatgaca ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt
     6841 ttcgcccata cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa
     6901 caatatgttc gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtccgccctg
     6961 aggtggcaca cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc
     7021 gccggaaacg tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc
     7081 atcaatatag atgatccctt tttctgcaag ttccacattg taatcagcag attgaagcaa
     7141 tttaagcaaa atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc
     7201 atctgcgatt gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt
     7261 accagaccct gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt
     7321 gttttcttgt gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga
     7381 gcgttttgcg cgatcttgtc caatcacgta gttattcaat acttcaagaa tttctagtgg
     7441 tttagggact tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc
     7501 tttacatagg tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt
     7561 cacttcttct tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt
     7621 gtcatacatg gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt
     7681 tacagtcttt tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc
     7741 aactgaccta tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat
     7801 cgtgtcacag tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga
     7861 tttattactt gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attcgattgc
     7921 ttttttcatt gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc
     7981 gattggcatg ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt
     8041 tgcttgaaga ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat
     8101 ttctgcttca ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata
     8161 gtacatttca ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg
     8221 atgtacttca tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc
     8281 catacggatc gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa
     8341 ttctttgcga tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt
     8401 tgcgaattcg tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt
     8461 gaagactgct tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt
     8521 tacatctaga ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa
     8581 tgagcctgaa cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc
     8641 atctacaaag ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc
     8701 tttgataact aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc
     8761 atctgttact tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac
     8821 ttctggtttc actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc
     8881 aacatcgatt tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata
     8941 agcttctggt aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg
     9001 gttgaatact tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt
     9061 cactttatta aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt
     9121 taaaacacca tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag
     9181 tttagaaatt cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa
     9241 ccctttcaaa ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat
     9301 ctttttcact gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt
     9361 ttttaaaaaa tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc
     9421 ctctacagtc agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata
     9481 aacaattgca gattgcaacg tgaattcttc ttttaacacg gaactaagca gcgcatcttc
     9541 attcccgtaa cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt
     9601 tggcagatct tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg
     9661 atggcattcg ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac
     9721 ctctggcgat acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc
     9781 gatctcatca atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt
     9841 attgtggagt gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg
     9901 gattttttca atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt
     9961 tctagcatat aaaaaatctt gtgtactgat taacaagtca aaataaaatt cagcaaactc
    10021 ctcattttga agatactgtt tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc
    10081 gtccaactcg taaagacaaa aaacaagcag gctattagcc tcaaagcttt ctttcaactc
    10141 gtaagctcgt tgaaataatt cttttgcctg ctgatattgc tggtttgcta gtgcttgttg
    10201 tgccactcgc attaaattct catatttttc tggaaaatca atcggcttca cgcagtgtcc
    10261 tcctcttgct catattcagc tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt
    10321 aaaaataact gaagcaccct ctaaaagcta gattttagat ctgactttta gaaggtgctt
    10381 tcaactgtta ctgcttttta ttgctcatca gctgttgact tcctggttga acgctttgat
    10441 cagattgatc gtaacaacac ttgtattagt gaaaaaatcg cccagatgtt gctttttctg
    10501 attaaagact gctggaagat accctaacat cagcaaagga aattttagaa tgaaccggca
    10561 agcaccttct ctaactaaaa cggttgacca gctaagttct tcttcatcaa agctgatgac
    10621 tcgaatacca aagatcattt tcccaatcgt ttggccatga ttgaatttcg tcaataaaat
    10681 aaaatacgct agataaataa gtaaggctgt gaatccgtag agactcagat aagaatctga
    10741 aaaattaagc caacctagat ttttggcaat accaagtgtt aaacgcgtaa tagctccgat
    10801 acacaaaaga tcgaccaaga aggcaaacat tcggataaaa aaacctgcat agaaatactt
    10861 aggaaaatca tttgcttttg gcggttcatt tcttgtgtcc gtatactttt tccatttacg
    10921 ttgtttcttt ttgatttctt cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt
    10981 ttgggaaaag ttggagtcag gtcctacagc aggctgctgt tgcttatctt tttcttgttc
    11041 cttattttca tttgaaagct ctgacatgct tactcacctc cgtaataata cattgctttt
    11101 ggtgattccg ctgtccctag attctcaaga acggataaga tctgactagt ctcagaaggc
    11161 ttcaatccct ggaattctgc tagtttactt cctaaccatg tactagcaaa cccagatggc
    11221 gtggaagaat attctactag ttccgcatct tcaagctgtt gttcctttcg tatagctttt
    11281 aaagcatctt caggaaatcc caattcatcg actaaaccta cttcttttgc ttggacacca
    11341 tcataaatcc ttccatcagc aatttttttg acttcttctt cagatttatt ccgtccctcg
    11401 ctgacgattg ctacaaaacg actatatgcg ctgtcgatat atgcttgaag caccgcttct
    11461 tcttcttttg tctctggtcg cgttcctgaa cccatatctt tcaacgctcc gcttttaact
    11521 gttgtatctt ccacacccag tttttctaga agtcctgaat aattcaatcc tgacatgatc
    11581 acaccaattg agccggtcac tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa
    11641 atatagtagc caccgctagc tgccatgttt ttcatgctga catacattgg gatatcatgt
    11701 tctttacgaa tcgtatccaa tttcttagcg atttccgcac tttcgtagac accgccacca
    11761 ggagaattta cttctaaaaa gatacctttg actgttggat cttcttcaat cgccttcagt
    11821 tgttccataa aatttttatg attgtaacct tcagaagcaa ataaacctga agaccctccc
    11881 gcagcaatcg ttccatctac tgataatttt acgatttttt gcgtactgtc tccctcttcc
    11941 aacacatttg gagctagttc ttcatcccca tataaccaac tgttcaatcc ctcaatttga
    12001 tcgttttcct gaggcttagt caaactagct gaaactaacg aaaccacaaa taatcctgca
    12061 gcaatcagaa ctgctgccca acgtctttta ttcattcttt ctcctttcta atacaagttt
    12121 tttatgacga tcacagtttc tgactgctca gaagtgtcga accgctctgc tttttcttcc
    12181 cgcgcatacc cggaaaacga ctcttttgtt aattcatctt ctaccgtaaa ctcacgagct
    12241 tgagcaactt ccaaggcatc aggtgcgatt tcttggatgc tatcccagcc agtatctaaa
    12301 aatattttcg tatcatctga tacaccagct gatgcttcga atttggacaa ttgttttttt
    12361 aacattccca gtgtcatgat ctcaattttt gaatccattt ttactcctcc tctatccgat
    12421 tactttctat tgtaccgaac tcctgaaaaa gatgatagat aaaaacccat tattttttga
    12481 cataaaaagg acatt
//
LOCUS       DACCXW010000085        12343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCXW010000085 DACCXW010000000
VERSION     DACCXW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.086"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..562
                     /locus_tag="IVY71_001994"
     CDS             <1..562
                     /locus_tag="IVY71_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986041.1"
                     /translation="NNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIA
                     ENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLT
                     TPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIV
                     TLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(707..1447)
                     /gene="truA"
                     /locus_tag="IVY71_001995"
     CDS             complement(707..1447)
                     /gene="truA"
                     /locus_tag="IVY71_001995"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7986042.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1638..2273)
                     /locus_tag="IVY71_001996"
     CDS             complement(1638..2273)
                     /locus_tag="IVY71_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7986043.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2420..3661)
                     /gene="sstT"
                     /locus_tag="IVY71_001997"
     CDS             complement(2420..3661)
                     /gene="sstT"
                     /locus_tag="IVY71_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7986044.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3859..4857
                     /locus_tag="IVY71_001998"
     CDS             3859..4857
                     /locus_tag="IVY71_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986045.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4870..6888
                     /locus_tag="IVY71_001999"
     CDS             4870..6888
                     /locus_tag="IVY71_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP7986046.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6948..7664)
                     /locus_tag="IVY71_002000"
     CDS             complement(6948..7664)
                     /locus_tag="IVY71_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7986047.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7891..8433)
                     /locus_tag="IVY71_002001"
     CDS             complement(7891..8433)
                     /locus_tag="IVY71_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7986048.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8438..8665)
                     /locus_tag="IVY71_002002"
     CDS             complement(8438..8665)
                     /locus_tag="IVY71_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP7986049.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8744..10624)
                     /locus_tag="IVY71_002003"
     CDS             complement(8744..10624)
                     /locus_tag="IVY71_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7986050.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10813..11427)
                     /locus_tag="IVY71_002004"
     CDS             complement(10813..11427)
                     /locus_tag="IVY71_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7986051.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11569..12129
                     /locus_tag="IVY71_002005"
     CDS             11569..12129
                     /locus_tag="IVY71_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7986052.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         3896 a         2447 c         1965 g         4035 t
ORIGIN      
        1 aaataattca tggaaaataa tggtctttac gtttcttgca ctaatattgc atctctcagg
       61 agtaagcaat tctatccatg ctaacaaaga ttcattaaca cttatagcac ctgaagacgc
      121 tgttgctata gctgaaaatt acaatcatac tgattatgca aacaaagagt caatctatca
      181 tcctgatatg attaataatg atcaatattt actaggtaac caggaaataa atccaacaac
      241 tcattttatg agtaacaaac ttacaattga attagataat tcgaataata aaaatactgt
      301 cttgacgaca cctatttatc gttataaagg gcaagtggct tctattaatg gcaaactagt
      361 acaaacaaaa ttatctaagt ttggcacaac tgaattaact attcctccag gtataaataa
      421 agtcgttatt acttatcagt acacaaaact agcaattgct tcaagatatt tatctatcgt
      481 tactttgatt ttatttttac tttatcgatt tactttttct aaacaaaaac aaccgagaag
      541 agaagtacaa cattctcact agatttttat ttaagaaaga ctttttagct gtacacataa
      601 acaggctaaa aagtcttttt ttatatttct ttaatttgct tcatacatta tttaacatcg
      661 atatttatac tgtgaaacat gcgtttcatc cattatcaaa gtatttttat cgataataaa
      721 cttcatccaa aaacagacct tgtgcaggga cggtttctcc tgcatgttgc cgcactttgc
      781 tttcaaatat ttcatcgatt gtttctatcg gcatggcacc tgtgccgatt tctagtaatg
      841 tccccatgat aatccgcacc attttatata agaatccgtc tccgacaaaa gtaaaatgaa
      901 gcatttctcc ctttcgttcg atagataatt catcaatcgt acgcactgtg gatttttttg
      961 attttttcaa tgcagaaaaa ccaatgaaat catgtgtacc cactaatttt tcgcaagctg
     1021 cattcagctt gtccatatcc aactcttgag ggaaataaaa gctgtggttt cgctcaaatg
     1081 ctgaaggaat cacgttgttc caaacataat agctgtattt tttgcccacc gcattatatc
     1141 ttgaatggaa gcgctcaggc atttcttcga ttcttttgat gacaatatcc cgcggaaggt
     1201 aacgattcat aaagtcgatc atttctgcca gtccaatttc tgtgtttgtt ttaaaatttg
     1261 ctacttgtcc tcgcgcatgg gtgccagcat ctgttcttcc tgatccaata atttctatct
     1321 cttcatttgt catctgatgc aatacttttt cgatttttcc ttgaatggtt ttatcgtcat
     1381 ctccaagtct ttgccatcct gaatatcttt ttccgtcata ttcgattgtc aacttgatgt
     1441 ttctcatatt ttagtggctc ctctgtcttc catttggtct tctctctacc tttttaagat
     1501 tgtagcacaa aatatagctc cttgcttctc taagacagaa gctatacaag aaaactgtgg
     1561 aaatatatat ttcagaaaaa caaaaaacag ccaccggatg aagtatccgc tgactgttca
     1621 atttgacggc tcaagtttta ctgtttgaga tgcaagatac tcaaccaggc aatgggcaca
     1681 taattaaaga aaaaactgat tctttggcaa aaacctattt tattcagtaa tggccaagaa
     1741 tttatagaag gaattcttgc cagtccataa atcgccgcaa aaagcaaact gatcacagac
     1801 agtccaaggt ataagtggct tacgtccatt tgcccatttt ttcgatagat caagaaaaga
     1861 agaactggaa acaataaaaa accactgtaa cctaaacctg atccgatatt atggacccat
     1921 gtcgagacat tccacgaaga ttcatgggta ttgatactga acaaacctgt aaagatacaa
     1981 tcacctatac catagaacgc gatggctgct gtaactaggg tagctaacac acgagatgta
     2041 ctaacaaatg cagcatacaa tgcaggcaat gacaggatga aaaagatccc tgaaaccaca
     2101 gaccaaataa gaaacgcttt tcttacagga ctggaaacct ctccaaatac actgatcacg
     2161 ctcttcattt gatctaattc aggataatac aatcctaata cgtacggagt caaaaaatca
     2221 ctcagcacgc ctaataataa aaagaaaaaa ccaaatttac gtaaaaaatc catgatacgc
     2281 ctccaataac aattacctat gcttttaatt gttattataa acgactttat cttgatttaa
     2341 cagaaacctc tatttacacg aacatctata taactcatta aaaactcgaa tcattttaaa
     2401 aatactaaat atctaacgtt caacgttcac ttaatccatc taaacaatct ttcaatttga
     2461 ttttttcccc acttctttta cgcgcgccta gctctcctat tgcagtaaac aacagatcac
     2521 ttgacgaatt taaggcagtt tccattgaat cctggataac accgataata aatccaatac
     2581 cgacgatttg catcgcgaca tcattagaga taccaaacaa actacatgct aatggaatca
     2641 ataacagtga accgcccgca acacctgaag ctccacaagc agctaaagca gacaaaatac
     2701 ataaaaggac tttcaaaaca aaaggtgctt caatccctaa agaatgtaca gcagtcaacg
     2761 tcatcaagga aatggtaatc gcggcaccgc ccatattgat cgtagcacct aacgggatcg
     2821 atatggaata agactctttt gtcaatttca atctttcagc caacatcatg tttatcggga
     2881 tatttgcggc agaacttcgg gtaaagaaag cgggaagtgc gctttctttc aaacaaaaga
     2941 aaatcaatgg atagggattt tctttaataa acaaaaacgt gaggaatggg tacacaacta
     3001 atgcgacaaa cagcatcgta ccgatcaaaa caagtaaaag ctgcagatac tctgccaatc
     3061 cagcgattcc agtcgtagca attgattgat aaaccaatcc taaaatccct atcggagcaa
     3121 atgcaataat cacttgtacg acttgtgaca acgctataga tatctgatcc accaactctt
     3181 ttgtcgattc tgaacgtgct cgaaaagcta gtcccaacaa tacagcccag aataagacgc
     3241 caaggtaatt cgcatcaata agagattgaa caggattttg tgtgacattc gtcaacatag
     3301 tagataaaac actttccaaa ctttgcggtg ctttttcaga tactgtttct tgtaaagtaa
     3361 taggaacagt aaaaattgaa cttacaataa ctgctacaag tgcagaaagc aatgttccca
     3421 ctaaatataa gatcaaaata ggcttcacga atgtcttatt cccaatttta tgcttggcaa
     3481 ttgacgccat cgtcaagaaa aaaaccagaa taggtgcgat tgccttcaag caactgacaa
     3541 acaacgttcc caatattgaa ataaatgtcc atgtggggaa aaagatacca aacaatattc
     3601 cgactaaaat accaacaaca atacgttgaa ctaacgacat atccaaaaac ttctctaaca
     3661 aatgaccacc ctttcacaat tttaacaatc attgtatcat tttatgcaaa gaaactaaag
     3721 gtataatttt ctaaaaattc taacaataca aacaaccatt tcttgctaat tttgtacttt
     3781 ttctgtctac acgttgtttt ttgagatatt ctaagttagg atgttattaa tgggcaactt
     3841 atcaggaaac gttttcttat gtttttgtcc aatttttttg tgaggtgttt attcttgaaa
     3901 aaaataaaga aggagagtta tggcttcctg acaatctttg tcgcttgtat aatgatggtt
     3961 ttacagcgta tattgatttc agcagtcact attcctacat atgcaacaaa ccctggtcga
     4021 ttctacatct atacattttt acaaacttcc gttgttattg gtgcaaattt gatcccactt
     4081 tacatgggtt accaatatca aaaaatcgcc cgtttaaaag ttttacatta tttaagtcgt
     4141 tttgccttta tattcctaat agccacacta gtttgcaata tcttttttta cgtacaagct
     4201 ggcagcttga atattcgtga ttattggctt atcctaacac ctatcagtca gaattacttt
     4261 acttatgctg tttcttgtac tttgcttttt tgtagtattc cttatacagt tggattttta
     4321 aataaattat cgaaaacaac aattaaatat ctactattat ttttaacaat tggattagtt
     4381 ggctgctcta ctctatttaa taaagacccg tttgcattac agaatggaca gagtatttta
     4441 tggatttttt atttattttt aatgggttat ggtttgaaag aatggaactg gaaagaaaag
     4501 gtccgctata agcttcctca agtatttatc agcttgattg ttttattcgg tttgattatt
     4561 ttgatgacac aaatttcact catcatcaga ggaaatacag atacagcgct gaggttttct
     4621 aaaccatatt ctctcttcgc aatgtattat actttctctt cgtttttatt tttagaaatg
     4681 ctatctgata aattaggtat aaaaggaaga ggaaattttc ttgctgtatt tttgattgtc
     4741 actcaagtta tatgtaattt ccctttagtt tcttatcgcg tatctacatt tatgaaacgt
     4801 gaatttccaa actcagggaa ggcatggctg ataaatatcg ggcagttttt tatttgatat
     4861 ttattagcag tgtccctcct agttatcttt ttattttata tacaaaaaat ccgtttcatt
     4921 catcgtttca tcgaatactt cgcttttgat tcgattgaac aattaactca gcgttggaaa
     4981 aaaagaattc attggctttt tgttcataaa agttattttt tagttgcagc atttttttac
     5041 tgctttacct tcgtgcagat tttcgtttta gaaccaaaag aaggttggaa agaaactatc
     5101 caggttgcct taaaaatatt tactcagcga caagcaccca tgatcctgac aatcatcatt
     5161 attatgggat ttttcttctt actattgttg atttccaatc gtttttggta cgccttgact
     5221 gctaccttga tcattaactt actgctcact atttcgacag taatcaaaat ggaaatgcgg
     5281 gaagagcctg tctttccttc agatttaaaa atgctgaccg gattgtcaga actattatcg
     5341 atggttagcc cagtcttatt aatcgtgggc ggattgatcc ttctcctatt attgattact
     5401 agtattattg tacaacgtag actacaaaaa cagtattcct tgaagattca ttggaaacgt
     5461 cgatttatca gtatcgctgt attattagga tgtttttcgg gtgtcttttt cattaatcac
     5521 aaaaattcgc cttctttctt gctgtttaat ttatttaaag taaacaagac tttctttaac
     5581 cagcaaggcg ccgtaaaaga taacggacca gtcattcaat tcctaaacaa tatcgacatt
     5641 aaaatcatgg aagaacctgc tggctattcg aaagaaaaaa ttaatggaat aatgaaaaag
     5701 tacaacacaa aggcagatga aatcaaccaa acaagaagtg actggttaaa caatcagacg
     5761 atcgttctca atttaagtga aagcttcagt gatcctgcac gtgtccctaa tctgacagta
     5821 cctacagacc ctattcctta catcaaaagc attataacag aaggaacagg aggattgatg
     5881 ttatccgtcg gttatggtgg cggaacagct aacatggaat gggaaggact gacaggcttg
     5941 agtatcagta acctgtctgc ttcgttagtt acaccttata cccagctagt ggagcgtcag
     6001 aaaataagtc caaatattac cgatctattc gatgaaaaaa tagccatcca tccttttaca
     6061 gctgctcttt atcgcagaaa acaagtgttt gataaatttg gttttgacag gttctattat
     6121 ttagacagtc cggataaatt aacttatacg gataaaatag aacaaagccc acgcatatcg
     6181 gatgattcag catacaaaga aaccttgaaa gctttacaaa gcaatacgga aacgtcacaa
     6241 ttcatccaat tatccactat gcaaaaccat atgccttatg atgattacta tagtgaattg
     6301 aactacactg ctgaaggaac tgctgtcatt cctaatagaa gaaaagaatt acaaacttat
     6361 atgcaaggcc ttcactatac cgatacagca gtaaaaaaat tcatccaaga aatcgacaaa
     6421 atcgataaac cgattacctt tgttttttat ggtgaccatt tacccgcaat ctatagtggg
     6481 aatgattcga aaaaatatgg attagaacag caccagacag attatttcat ttataacaac
     6541 aagtatagtc gtgaacaagc agctacgtta aataaagaag ttgttgctcc taataacttt
     6601 ggtgctatgg ctcttgagca tgcgaatatc aaagtaactc cttactatgc gttgttgaca
     6661 gaagtatcta atcatctacc tgcattaaca attgatccta cagaaagctt gtctaatcgt
     6721 tttaatggaa aacaggtctt tgttacagag gatagtcaaa ttatccaaga aaaaaattta
     6781 actgagaaac aaaaagaaat tctagaagat tatcgtctga tacaatatga tcttgttgca
     6841 ggtaaccagt acagtgcaac atgggcgaca caaaaaataa aagactaaca aataaactga
     6901 gcaaggggtt gtgataaaag acatgtcaca accccttgtt tatctcatta atttgcttgt
     6961 ccataaacga tttgattaaa gccatttttc aattcattat tcgctgtctc gttgaagtaa
     7021 agataattat cgggattccc ctgattttgt tgtgtcacca atacttttgg ctgattttgc
     7081 tgaaacccga atagatagac gtgctttttc agatatggtt ctgtctctgc atcggaatag
     7141 acggctgtga tttgatagtc tgcttgacct gtcccacttt ctgaccattc tgcagaaaca
     7201 ggcactccac caatagccat cttccactcc ccgttcaaaa tagcttgagg gactttaaga
     7261 ccatataagt ccacactcat ttgattcgta taacttttgt attgttgtcc cattgtttgg
     7321 ccccatttgg tcataaatgc ctctaattgt gcggatttgt ctgcattcca cagtgtttgt
     7381 tcaacttgtt cgctgctttc tgaagaggaa gatgaagcct ctacagaaga acttgaagta
     7441 gatgtttgag ttgtttcagt cgttttggaa gactctgacg aagtagaggt atctgctgtt
     7501 tctgtacttg ctgcagttga cttgctctct gtgctagttg ttgtagtagt tgcaactgtt
     7561 gctttcgctg tttcttttgt tgattctttt ttcgattcat tcccgttggt acaaccagct
     7621 aacaaaaaag ctagaccaat tgttactaaa atcctttttt tcaattaaaa aactcctttc
     7681 gattactccc acttctttat tctatcaaat aagcaatgtg attttattga tttttcgtat
     7741 tttctcatca atctttcaaa tcttctaaaa actgagaagc acccgctgat gttgtctgta
     7801 atacatcagc gggtgcttct tttattttaa tcttcgtgat tttccaattt acctcacgct
     7861 ttatatttgt atttgaagaa acatagactt ttagtcatca tcgtcttcct catccaaatc
     7921 ttgtcggaca gtttttcctt ggagtctttg tacttggtag atgatttctt tattgaaact
     7981 taaaagttca acaagcaatt gttgcaagcc atatttttct tctttttcag ctaatttgat
     8041 tgcccgtgtg ataaagacat tttggaaatc aaaatctttt gcaaagtttt ccaacattgt
     8101 tgaggctggt aaatatttgt tttgaccatg ttcgtaaatt tttccataac gcatgacttc
     8161 ttctaaagta gacggaacga tttcattttc aatcactaac tcatgagaga ttttattgaa
     8221 ccatctcgct tcttgatcag catattctga aaaaagggta cttaattctt gcgaagatgt
     8281 acccaaaaca tacaattgtg cttggatcaa tttcatttct tctaatttaa aatttgctaa
     8341 tacatgcccc accatggcgc cagcagttgg tttatgatgg tcgatatctg cttgttttgt
     8401 ttcttgaaca aatttttctt ccactatatt cattgattta cgcctctttt acttttgctg
     8461 attggacttc ataacctaat ttttccacaa ctgcagctaa ttgttcaggg tggacttcat
     8521 ctggatcaaa gtttgctttg attttgctcg cattaaacaa aactttcgta ttgcttaccc
     8581 caggctgttg agaaaccgca tgttcgattt tttgcataca gcttggacaa gctagtgtat
     8641 ctaattgcat aataattttt ttcatcgtag ttcttccttc ctttttcttg atagtcttat
     8701 tctagattac agcttccata aaaaaattga ttcttatcaa gttttacgat ttttcttctt
     8761 tgctttcgtg tccaaaatac aataaacgca tcgcgttaaa gataacgatc aagatactag
     8821 cttcgtgaac gaacatccca ctagccatgt aaataaaccc ggcaaataag ccgttgagta
     8881 aaaataagac gacaccaatg gcaatcacta cattttcacg ggtatttaaa acagttttct
     8941 tagccaagcg gtaggcatga accaacgatt caaaattaga ttgcatcaag acaacgtcac
     9001 tcgtttcgat agcaacatcc gttcctgatc ccatagcaat cccgatatca gccgcagcca
     9061 aagagggaga atcattgatt ccatctccaa taaaagcaac gcgcaatcct tgttcttggt
     9121 attttttcac aaattgaacc ttttcgtctg gtaataattc agcatggact tcatcaatgc
     9181 ctaacatatc agctacataa tcagcagtca tctgattatc acctgtcagc atcactaact
     9241 gttttgctcc tgcttgtttc aacttagcta attgttcagc gacttctggt cgaatcacat
     9301 ctgacacacc tatgatttga gtgatttttt tgtcgattgc tacaataatg accgagctgc
     9361 ctgtttgttg taattgatgt aaatcttgta cttgctttgg tgtgagagaa agtttgtgag
     9421 cggcaatcaa tttggtgttg cccgcacaaa ctgtatgacc atcaatctca gctgtgattc
     9481 cttgtccctt gattgtctga ttatctttga ctgctaattg ttgaaaatca atgtctttct
     9541 cctgagcata atggatcacg gcgcgcccca gaggatgatc ggataacgtt tctaatcgag
     9601 ccactaaagc taagctgtcc ccttcatcct ctgtatagtt cttgatagtg gatacagcag
     9661 tagttccttt ggtcaatgta ccagttttat caaagacaaa agtatcgatt ttggaaaaag
     9721 tatccattgc ttcaccgcct ttgactaaga tgccccgttt cgctccatta ccgattcctg
     9781 ctacattcga aacaggcgca ccaatgacta aagcgccagg acaaccaaga accaaaacag
     9841 tgattgctaa acgaaaatca cgactaatca gaccaacaac caacgcaata agtaaaacgg
     9901 ttggagtata atactgagaa aatcgatcaa tgaatttttc cgcatgggat ttggtatctt
     9961 gtgcttcttc tactaattcg ataattttgg caaaggtcgt atcatctccg accttagttg
    10021 cttcaatcgt cacgtagcca tctgctaaaa tcgttccaga gaagacttca tctccactga
    10081 ttttgacgac ttgtcgtgat tcaccagtca ctgcggcttc atccgcatac cctttcccgt
    10141 caatgactcg tccgtccaca ggaatagctc caccagtttt tactaagacg atatcgcctt
    10201 cttccacgtc atcgatttcc acttcttcag tcgttccatc ttcttggaca acactagcag
    10261 tagatggggc catctcggtc aaagccttga ttgactcccg cgttttggtc aatgtttttt
    10321 gttccagata gctaccaaac aaaaacaaga atgtaacgat agctgactcc ttgtattctc
    10381 cgatcataaa ggcaccaagc acagcaatag taacgagtaa atcaattgag ataactttga
    10441 ctttcactgc ctgatacgca tggatcataa taggtatgac cgcaataata gaagctatgg
    10501 ctagagcaat gttatagaac aagacaaaac cagtaaattt tccgataaaa ccaaaaataa
    10561 tcaacacagc tgataaggcg gtgatatgat ttcgatgctc ttggatccac ttttgtaatt
    10621 tcatctttct ccctcttttc ctttcattct tacgagacta agtatagata cttatccatc
    10681 ataaaaaatt gaccactatc aagtttagtc aaaaaggcgg ttttcttttg gtagaataca
    10741 ctacaaaaaa tagttagcaa gaagactcac taaaagtgat cttgccaact attgtcgttt
    10801 ctatcgaaat tttcattgaa tgattttcct gtacagcata catcggagat ctatttcttt
    10861 atctaagatt tttatcttgc taattttttc ctttagtctt tcaaatccac gtcgttcata
    10921 gaattgatac gtacagccac tatctgtaaa aaggaatatt tcttttcctt tctctctttt
    10981 ttcaaattct gttagcaact tggtaccgat gccttttgtt ttcaaatcag gatttgcagc
    11041 taaaaaaatg atctctccat ctggcgaatg cttctttaaa tatgctgcaa gcatttgttc
    11101 atttgctgtc tcgtaagctc ctttactttc tttagcaaat atattttgta aaaaatcgaa
    11161 tatcttaaca tatatttgct gtccaaaaga acgatgtttc tttttttctc cttttatctc
    11221 cgccagcaaa acacctgcca actcgtcctc tgcatacaaa gcaagaattt gagttgctcg
    11281 attcaactcc atatacaaaa aatatcttcc gtaaagattc agcagaagtt cattttccat
    11341 atatctctca aagttcattc cttcaacagc aaaatagata gcttttttgt aatctttctt
    11401 ttctaagggt ttaaaagtta gtttcatcat ttcgcctcct caacttcaat gattgtgtta
    11461 tacttacatt ataatagaca cagtgtctac tatctataat caatcatcta taagtttgtc
    11521 gattaagcga cagctttgct ttgatatgtc ggataaggag ataggtaaat gaaaaagaca
    11581 gatagacgcg tcaaaaaaac agaaaaagct ttggctgaaa cactatccac attattagta
    11641 aataaaaaga ttcaagcaat cactatacgc gaactaacag aaacggcaga tgtacatcgt
    11701 tcgacttttt atacacatta taaggatatc tatgatctat atgatcaatt agaaagcaac
    11761 ttttttcggg atctaaatga aattctctct tatgatccag cccatagcta tgaggaacta
    11821 tacactcgct tgattgacta tgtttacgca aatacttttt tatatcgctt atttatcctc
    11881 aaccatggtg atatttcgtt tcagaagaaa gttaaacaag ttattgaaga aaactatctg
    11941 aacatatggc tatatgaaga caagaaaacg attatcacag aagaaatgag attctttact
    12001 acgtaccacg tccaaggttg tctatccgtt ctcaatcggt gggtagaaag caattacaag
    12061 tactccaaaa aagaattatt agaactctta cagaaactga atgatcacat agagaaaatc
    12121 atgccttgaa taaaaacata ctaagcttag ttaagaagaa ccgtaacctt ctcgtataac
    12181 aactgaatct tatttagcca aattgtttac tgaagatttt ttgagtaaaa tttctataat
    12241 tcctacttat tgaaaagtag tttgtagtgc tgcttctccc tctctaagtg atatatcttg
    12301 ttgtaccaat caaattcacc tcatcattaa taaattactt tta
//
LOCUS       DACCXW010000086        12078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCXW010000086 DACCXW010000000
VERSION     DACCXW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.087"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..583)
                     /gene="frr"
                     /locus_tag="IVY71_002006"
     CDS             complement(26..583)
                     /gene="frr"
                     /locus_tag="IVY71_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7986053.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(586..1308)
                     /locus_tag="IVY71_002007"
     CDS             complement(586..1308)
                     /locus_tag="IVY71_002007"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7986054.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1444..2325)
                     /locus_tag="IVY71_002008"
     CDS             complement(1444..2325)
                     /locus_tag="IVY71_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7986055.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2424..3206)
                     /gene="rpsB"
                     /locus_tag="IVY71_002009"
     CDS             complement(2424..3206)
                     /gene="rpsB"
                     /locus_tag="IVY71_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7986056.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3565..4044
                     /locus_tag="IVY71_002010"
     CDS             3565..4044
                     /locus_tag="IVY71_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7986057.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4261..5352
                     /gene="phnW"
                     /locus_tag="IVY71_002011"
     CDS             4261..5352
                     /gene="phnW"
                     /locus_tag="IVY71_002011"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7986058.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5345..5473
                     /locus_tag="IVY71_002012"
     CDS             5345..5473
                     /locus_tag="IVY71_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7986059.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5477..6022
                     /locus_tag="IVY71_002013"
     CDS             5477..6022
                     /locus_tag="IVY71_002013"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7986060.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6476..8167)
                     /locus_tag="IVY71_002014"
     CDS             complement(6476..8167)
                     /locus_tag="IVY71_002014"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7986061.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8587..9534)
                     /gene="arcC"
                     /locus_tag="IVY71_002015"
     CDS             complement(8587..9534)
                     /gene="arcC"
                     /locus_tag="IVY71_002015"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7986062.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9649..10668)
                     /gene="argF"
                     /locus_tag="IVY71_002016"
     CDS             complement(9649..10668)
                     /gene="argF"
                     /locus_tag="IVY71_002016"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7986063.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10759..11988)
                     /gene="arcA"
                     /locus_tag="IVY71_002017"
     CDS             complement(10759..11988)
                     /gene="arcA"
                     /locus_tag="IVY71_002017"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7986064.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3291 a         2517 c         2191 g         4079 t
ORIGIN      
        1 ccgcccctct gtttttatga ttttattaaa cttccaataa ttctttttct ttatcagcag
       61 cgatcttatc tacttctttg atgctgttat ctgtcaatgt ttgtacgtct ttttctaatc
      121 cgcgcaaatc atcttcggta atatcaccat ttttttgttg ttttttatat tcatcgatcg
      181 catcacgacg gatgttacga atcgcgattt ttgcattttc tgcttctttt ttaacatctt
      241 tagctaattc ttttctgcgt tcttctgtca gttgcgggat aactaagcga atgacatttc
      301 catcattggt tggactgatt ccgatatcac ttgccataat agctttttca acatcttgaa
      361 gaatgctctt gtcaaaaggt gtgatcatta aaacacgtgc ttctggaatt tggattgatg
      421 ccatttggtt caatggtgtt ggcgcaccgt agtagttcac agtaatacgg tccaaaaggc
      481 tagcgttcgc acggcctgcg cggatttgcc caagttcacg ttgaagattt tgtgctgctt
      541 ttttcatttt ttcttttgct tctgtcataa ttgcatctgc catttttatt tcccccttac
      601 agttgttccg atattttcgc ctaagattgc acggcgaata tttccgtgtt catttaaatt
      661 gaagacaagc agtggaatat cattatccat gcttagtgaa cttgcagttg aatccatgac
      721 ttgtaatcct ttggcaataa cttccagatg agtcagttct tcaaatttta ctgcatttgc
      781 atctactttc ggatcagcag aataaacacc gtctacattg tttttagcca tcaggataac
      841 atctgcgccg atttctgccg cgcgtaatgc tgctgttgtg tctgtcgaga agtacgggtt
      901 gccggtaccg cctgcaaaga taacgatccg tcctttttct aaatgacgtt ctgctttacg
      961 gcgaatatat ggctctgcga tttgacgcat ttcaatcgaa gtttgcacgc gtgtcggtac
     1021 acccacgttt tccaacgtat cctgtaaagc taaggcattc ataactgtag ctaacatacc
     1081 catgtaatca gcttgagcac gttccatgcc catttgtgct ccgatttgtc cgcgccagat
     1141 atttccgccg cccacaacga ttgccatttc aacgcccagc tcgtgaactt ctttgatttc
     1201 ttggatgatt tctttgatta ctggtggttt gattccaaat cctgcgtccc cagctaaagc
     1261 ttctccactc aattttaaga ctacacgctg atatttcggt gtgaccatgt tcattcctcc
     1321 aatattttct attgccattt taccatactt agatgatttc gacactctaa gactggtttt
     1381 tcttgttttg cttaaaaaaa gggagcgcac aaaccgtgcg ttcccgtctg ttattcaaca
     1441 gaattatttt ttcacttggc tcataacttc ttctacaaag ttatcttcgc gtttttcgat
     1501 accttcgcca acttcgaaac gtacgaatgt tttaacagtt gcgccttttg atgcaacgta
     1561 tttttcaaca gtcatatcag gatctttaac gaatggttgg tccaccaaag cgatttctgc
     1621 tttgaatttc ttcaagcgtc cttctaccat tttttcaacg atattcgctg gtttgccttc
     1681 gttcaatgct tgttctgtaa gaacagtttt ttcatgttct aattcagctt ctggaatttg
     1741 tgattcgttt acataacgag gattgatagc tgctacgtgc atagctacgt ctcttgcaac
     1801 tgtttcgtca gttgttccat ctagaacagt caatacggca atacgtccgc ccatatgcaa
     1861 gtatccacca aatgcagcat tgtcgtcttt ttctacaact tcaaagcgac ggaagctgat
     1921 tttttctccg ataacttgag ttgcttcgat caagtcagat tcaattgtac ctttatctgt
     1981 tttgattttc attgcagctt ccatgtctgc tggtttgttt tcagcaacaa gttctgcaat
     2041 ttcttttact aaatcttgga acatttcgtt tttagaaaca aagtctgttt ctgagtttac
     2101 ttcaacgatt gctgcagtgt tacctttcac agcaactgaa gccaatcctt cagctgcaat
     2161 gcggtcattt ttcttcgcag cctttgccat tcctttttca cgtaaaagat caactgcttt
     2221 ttccatgtcg ccttctactt caactaatgc ttttttcgca tccatcattc ctacgccagt
     2281 catttcgcgt agttctttta ccattttagc tgtaacgtct gccatgttta actcctcctg
     2341 tgtgtttttc tttttaaaaa aagctgcttc aaaggagagt gaggcttgtc gcccagcctt
     2401 tgaaacagct ccatcattca aaattattct gctgaagaat tgtcgccttc aacaacatct
     2461 acgatttctt cgattgatgt tgctgtgttt tcttctgtga aatcttcttc aactacttga
     2521 tcttcacctt ggtttccttc gatgaaagca tcagccattt ttgaagtaat caatttaacg
     2581 gcacgaattg catcgtcgtt tgaagggatt actacgtcga tttcatctgg atcgcagttt
     2641 gtatcaacca tagctacgat tgggatattt aatttgtgtg cttcttgaac agcaatacgt
     2701 tctttacgtg ggtcaacgat gtacattaca tctgggattc ttggcatatc agcgataccg
     2761 cctaagaatt tttcaagacg ttcacgttct ttgttcaaac cagcaacttc ttttttaggt
     2821 agaacttcaa aagttccatc ttcttccatt gcattgattt gtttcaaacg gctgatacgt
     2881 ttttggatag tatcccagtt tgtcaatgtt ccgcctaacc aacgatggtt aacgaagtat
     2941 tgtcctgcac gagttgcttc ttctttgatt gcttcttgtg cttgtttttt tgtacctacg
     3001 aataatgcta cgccgccttc ttcagcaacg ctcttcatgt aatcataagc tgcatctact
     3061 aatttaactg ttttttgcaa gtcaatgatg tagattccgt ttctttctgt gaagatatat
     3121 ttcttcattt ttggattcca gcgacgagtt tggtgaccaa agtgtacgcc ggcttctagt
     3181 aattgtttca tagaaattac tgccatgttt gtttcctccg atttggtttt atttttcctc
     3241 ttcttatctt ctaccttgcc ggcaaacttt ttcaagcacc taccgacaat ccaataagaa
     3301 tgtggatttg ctgatgcata aagcaccctc agctattata cagacaaaag atccgtatcg
     3361 caagagcaaa tatacttttc tgctcaacat tcaatagttt acctgaaaga taaaaagaat
     3421 tcaagaaaaa cagtagattt ctctttgttt ctttattatc ctttttccat aaaataattt
     3481 taggtataaa agacgaatat tattgggcaa tccttctttt ttttgttatg atacatgtga
     3541 aatctttcgt aaaggagaaa gaaaatgcgc aaagctcaaa gacatttgct gattaaacaa
     3601 gtcattgaag aattcccgat ccgtacgcaa gaagaactat taaacaaact tgggcattat
     3661 ggcgttaccg ccacgcaggc aacgatttct cgagatatcc gtgatctaaa aatcgtgaag
     3721 gcacctgatg aaaatggcgt ttcccgcttc gtattgtttc gcgaaaaagg aactcctgag
     3781 actaggactg aggaagagga tcggctggtt caaatgattg aagacattgt attgaaagtc
     3841 gaacgagtcc actttttgac gatcgtcaat accttgccag acaatgctca tttatttgca
     3901 tctgttttag atgagatcaa acctccctct attgtcagta caattgctgg attcgatacg
     3961 atcatggtca tctcggaaaa tgaggaggac gcaaaacgag tagaagaatt tcttcaccat
     4021 ccaactgaag tatcactttt ttaatcagag cacgtattcc taatatggaa tgcgtgtttt
     4081 tttattttca atattttcct tattgaataa acctttacac tctttacaaa aatacatcat
     4141 tttttattca aatttttcaa caccttgatt gaatcacaat aatcatcatt tatattggat
     4201 gttaacaaga gcagcttctt gctctttgtg tatttcaaaa aagaaatgaa aggaaatcat
     4261 atgtataaat tattaacttc tggaccattg acgacttctg aaacagtgaa aaatgctatg
     4321 ctgatcgatc attgtacttg ggatcaagaa tacaaagaaa ttacacaatg gataagaaaa
     4381 aaattattgg atcttgctca agtatcggaa ggaatataca caacggtctt aatgcaaggt
     4441 agcagctcct tcggggtaga atcggtatta agttctgtga tcaggccaga agatactgta
     4501 ctgatttgtt caaacggcgc atatagacaa cgaatgatca acatgtgcga atgtctgaaa
     4561 ctgaactata ccgtttatga ggtatcggaa catcaaatcc tccaagctca tgtgattaca
     4621 cagctgattg aagagaatcc gtctatctcc gcactagcaa tggtccatag cgagactact
     4681 tccggtattt tgaataattt ggaagagatt gctgctgtca cacaagaaag agatcttttg
     4741 tttatagtgg acgcgatgtc aagctttggt gggataccga tttgtgtagc cgaactaggg
     4801 attgattttt tgatttccag ctcaaacaaa tgtattcaag gtgttccagg cttttctttt
     4861 gtacttgcta ataaagcgga actggaaaaa cgaaaaaatg ttagccgaag tttgtcactt
     4921 gatctatatg accagtaccg cgtgatggaa aacgagaatg gcaaatggcg tttcacttcc
     4981 cctacccata cggttttggc ttttcaacaa gcgctgaagg aactagaggc agaaggagga
     5041 atcgaaactc gatattcacg ttatctcgcc aataatcatg tgctacgaca aaaaatgaca
     5101 gagatggggt tccagccttt cattcaagaa tatcaggggc cattcatcac ctcttttctt
     5161 taccctaagg actggcgatt tgattttaat catttttatc agtatttaaa gcaaaatgga
     5221 tttgtgatct atcccgggaa aatcagcgat catgaatgtt ttcgtatcgg aaatattggc
     5281 gagatctatc cggaagatat tttggaactg acagcaatta tcaaagacta taaggagaac
     5341 gttaatgatt aaaactgtaa tttttgattg ggcaggaaca actgtggact ttggctgtat
     5401 ggctcctgtc cacgctttta gaaatgcttt tctagaaaaa ggaatccaac tgactgacaa
     5461 agaaatccgc taaccgatgg gaaaattaaa atgggatcat atccaacagc ttctttccct
     5521 cccttctgtc aaaaaacaat ggatagctat ttatggacaa ctacctcagg aaaaagaggt
     5581 ggaggaattg tatcaacaat ttgaacatta tctgtttaaa gatttggtca agcattctac
     5641 cttgaaaccc gacacattgg aaacgatcga acggttgaga aacgctggga tcaatattgg
     5701 ttccacgaat ggatttaatg ctcctatgat gacaatcgta gcagaaaccg caaaaaaaga
     5761 aaggtattca ccagacttca tggcaacttt tgaagacgtc gaagggtatg gtcgaccgtt
     5821 tccttatatg atccataaaa acatgcagta cttcaaaaac aagtcggttg atgaagtaat
     5881 caaagtcgga gatacggtct cagatatcca ggaagggaaa aatgccggag tgttgacagt
     5941 aggtgtaatt gaaggtagtt cgctaatggg gatttcttat gatgagtacc aaaaattaga
     6001 gatggggaaa aagatcgttt aaaacagtct gttgctctta gattccaaca agccggcgct
     6061 gatatttgtg taaacaattt aaaagagtta acagaataca ttttgtacgc tgacatgaat
     6121 ccagaatgac ttttatacaa aataagaggt tgtgaaaaag ctgtcctgta tcaagtaata
     6181 aaaacagcca agcaaaaata gctcctcata tttttcgttt ggttaggctt attaccgcag
     6241 atgcaagctt ttgaacaccg tttattcgaa aaaaaaaagg gagctgtgac acgacttttg
     6301 tcacagtccc cttatttgca aacaaacagt gtttaagaga caacctctcc actaaacggt
     6361 gttcattcag ctgactctcg gtatttgctc aaaaaacgat aaaatttgaa aagctatttt
     6421 ctcttttgtt tctctaatac tcggatcaga acgctgattt cacattctct ttttcttaca
     6481 tcttatctgg tgcatgaagt cctagcaaac gaaggtcttc tttcaataat acagtcactg
     6541 cataaactag agctaagcgt gcttcttttt gttcatcatc agctaaaatc ttcgtatgag
     6601 catagtattt gttgaatgct tgtgctaatt tgattgcatg tttcgcaatc actgacggtt
     6661 cgtatttttc cccagcagac aagaccgttt cagggtattt ttgaactaat ttaaccactt
     6721 cccagctggt atcgtcattc aatgcatagt ctgctgtttc tgaagggaca aatcctgctt
     6781 tttccaacag gctgactgca cgagcatgtg tatattgcac ataaggtcca gtttctcctt
     6841 caaatcgaac aacttcttcc aggttaaagt caaatgtatt cagtcgatcg tttttcaaat
     6901 cgtggaaaat gactgcccca accccaactt gtttagctac agcatctttg ttttccaaat
     6961 cagggttttt ctcgctgatt tgttctttag cagattggat tgcttcattt aatacttctt
     7021 ctaataagac gatctttcct ttacgagttg ataatttttt accaccttgc gtgatcaaac
     7081 caaatggaat atggtgcatg tcgtcagacc agtcaaagcc catttccttc aagacagctt
     7141 tcaactgttt aaaatgatag ctttgttcat tccctactac atatagagat tgtttgaagt
     7201 cgtaagtacg tttacggtac aatgcagcag ctaagtcacg tgtaatgtat agtgtcgcac
     7261 cgtctgactt cttaatcaac gctggattca ggtcataagc agataggtca acgatctctg
     7321 cacctttatc ctcgttcagc aaatgttttt cttctaaaag tttgacgatt tcatccatct
     7381 tgtcattgta gaaagcttca ccgttcaacg aatcgaaacg tacttccagc agatcataga
     7441 ttttattgaa ttctttcatc gattcatcac ggaaccattg ccataattga atagcctctt
     7501 tatcgccttc ttccagacgt ttgaaccaag cacgagcttc ttcatttaat tctggttctg
     7561 tttcagctac ttcatggaat tggacgtata aacgaagcaa ttcattgatt ggttctgctt
     7621 taacagcttc ttctgtaccc cattttttgt aagcgacgat cagttttcca aactgtgttc
     7681 cccagtcacc taaatggttg atgcggattg gctgataacc gattttttcc ataatgaaac
     7741 cgatcgaatt accgataact gtcgaacgca aatgtcccat tgagattggt ttggcaatat
     7801 taggagaaga catgtcgatt ggtactgttc cttgattccc gatgttgcta tccccataat
     7861 gttctttttc tttcactact gtttgcaaaa cttttttgct gataagttct ttattcatga
     7921 aaaagttcaa gtaaggtccg acaacttcga ttttttcaaa gttggcagaa tcgatttttt
     7981 ctgctaaatc tgcagcgatt tgctgtggcg cttttcggta aacttttgct aatgaaaagg
     8041 ctggaaatgc aacatctccg tgttcagctg attttggatt ttctagtaat tgctcgactt
     8101 gttccagtgt taaatcatct ttgacagcgt catagacagc ttttgcgaca agatctttat
     8161 tattcatctt tattcctcct aaagtgttcc cttgattgtt aaacaaaaaa aatctctagc
     8221 gacggcatac ttagtatgcc atcactagag acgagatttc ccgcggtacc actccaattg
     8281 tcctaaaaaa ggaccagctc catctgatat cggtagacga ccgtctgttt aaaacagaat
     8341 tctccaagtg cgcttcgttg tatcttcttc attcggcttc caccacttcc gaattcgcta
     8401 gccaagaatg atacaagtac tctcttgttc aacaaatctt agtcttatta aactttcttg
     8461 aattatatca gaaaaaaaaa cgaaaggcta gtcgtttcat aaaaaaacaa tccagatggc
     8521 ctaaaggaga agaaatacca cctggatagc tataaatcat ttcgtaaata cgaaagactt
     8581 aacaaattaa tcagctacga taatcgttcc ttcttcagaa gctaaaagat tctcgatatt
     8641 ttctaaagaa gtgatgattg ccttagcgtt tggttttgct tcaacaaatg caatagctgc
     8701 ttctactttc ggaagcatgc ttcctggagc gaattgcttt tcatcaatgt attgtttcat
     8761 ttctgaaaca gtaacagttt ctaatttctt ttgatctggt ttttggtaat tgacatacac
     8821 attgtctact cctgtcaata cgatcagcaa atccgcatcg atgatttcgg ctaatttttc
     8881 tgaagcaaaa tctttatcga ttacggcttc tcgtccttct aatccagtag ctgtttctac
     8941 aacaggaatc ccaccgccac caacagagac tgtgataact ccttgatcga ccagcgtttc
     9001 gatgacacgt gcttctttaa tcgagatggg tttaggggaa gcgactactt ttctccatcc
     9061 acgtccagca tcttctttga atgtcacagt ggagtcagca ttcatttgtt ctttggcttc
     9121 ttcttccgtg tagaatggac cgactggttt acttggattt ttgaatgaag gatcattttc
     9181 atccacaata acttgagtga ctaaagaaac cacatcttta tcgatccctt tttccttcag
     9241 tacttcgccc atggcgtttt gtaaccagta accgatagat ccttctgtca ttgcaacaca
     9301 tgtatctaaa ggcattgccg gtgttttctc agaatcagca gcttgctgct gaatcaatag
     9361 gttaccgact tgcggaccat ttccgtggga gatgatcaat tcatctcctt gttcgataaa
     9421 tttcactaaa tactttgctg tttccattaa cgcttcttgt tgggcttttg cacttgcatc
     9481 cgtagacaag atggcattgc cccctaaagc gacaactact tttcgtttgg ccatttcatt
     9541 cgtcctttct tcagttctac cgaattttct agataacaaa acgagccagc gttttgtgat
     9601 tttccctcaa tgctgactcg ttttttcgtg agatgaatgt ggttatgctt aaacgcgagg
     9661 gataaacaag ttacctaatg ttgctgccat gatcgctttg attgagtgca tacgattttc
     9721 tgcttggtca aattgacgag catatttgct gcggaagact tcatctgtta cttccatttc
     9781 agtgatgcca aagcgttctt tcatttgttc accgtaaaca gtgtttgtat catggaaagc
     9841 tggcaagcag tgcaagaaga tcaaacgatc tgtgttgtgt gttttttcta ccatttccat
     9901 attgatttgg taaggtttca acaatttgat acgttcttcg aatttatctt cttcacccat
     9961 tgatacccat acatcagaat aaaggacgtc tgctccgtct acacctttat caacatcgtc
    10021 ggtaatcatc aattgtgcac cggatttttc agcaaagcct tctgccattt tgacgatttc
    10081 ttcttcaggt tgtaattctt ttggtgcaac gatacgcata ttcgctccta aaattgcacc
    10141 tgtgactaat aaagagttag ccatattgtt tcgaccatct ccgcagtaag caacagtgat
    10201 accttctact gttccaaagt tttcttggat agttaaaaag tcagcgatca tttgtgttgg
    10261 atgccattca tcagtcaagc cattccaaac tggaacacca gagaattctg ctagttcctc
    10321 taccattttt tgactgaatc cacggaattc gattccgtca aacatacgtc ctagtacttt
    10381 agctgtatct tctgtggatt cttttttacc taattggata tcatttgcac ctagatattc
    10441 aggatgtgca cctagatcga tcgctgcagt agtaaatgca gaacgagtac gtgtagatgt
    10501 tttttcaaat aaaagcgcaa tattttttcc ttctagatag tgatgtggga taccacgttt
    10561 tttcaagtct ttcaagtgtt ctgaaaaatc gatcaaatat tgtaattctt cttttgtaaa
    10621 gtctttttct gctaataagc ttcttccttg gaatactgat tctttcatat taaacattct
    10681 cctttagatt gtttttattt aagattaaag aggaaatccc gacggcagct gacagtaaga
    10741 ctgccgtcag gaaacaaatt acttcaagtc ttcacggaca agtggttggc tcatgcagcg
    10801 tggaccacca cgacctcgtg acaattcact tgaattgatt tctaacactt tgatgccata
    10861 gctacgaagc aattcgttcg atacataatt tcggttataa gtgactacaa cacctggagc
    10921 gattgccaat gtgtttgatc catcattcca ttgttctcga ggtgcaacga tctcatctcc
    10981 attaccagtt gggatcagta caagatcatc taatccaaga gcagctttca tcgtttcgtg
    11041 taagttatct gaatgagtaa tcttgatatc atcaccatct ggttcgattg taaagacatc
    11101 catcttaccg tctttacttt gaattgctgg atggatagtg aatttatcgt gatctaccat
    11161 tgtaaatacg gtatccaaat gcatcattgc acggttgttc ggaattttga ttgccaacac
    11221 tttttcaaat cctgaatttt ttgcgaatag gtggcgtgct aatttttcta aagctttcgc
    11281 attagttcgt tgagaaatcc caacagctac tactttatcg cttaagatca attcgtctcc
    11341 gccttcgata tgatctggat tttcacgatc caaccatact tcgattcctt tatttgcaaa
    11401 tcttggatga tgtttcaaaa tatattctgt aaacatcgat tcacgacgac gtgcttcaaa
    11461 agtcatctta ttgactgtca tcccatttcc cattgatgcg gaagggtcgc gtgtaaagta
    11521 caagtttggc attgggtcca tgtaaaatgg atagtcatcg tctgctgaca gatcatatag
    11581 actgctagaa cgtacttcga tatcttttgt acgaacccca gccatgattt tatctaccat
    11641 ttcttgcgtt tccattgata gaaggaactc atgaagaccc tctcttacag cattcgatgc
    11701 aatatgagat tcatccaaca ttttattcaa gaattgttct ttgacattgc ctgcgtcaat
    11761 cgcttctgcc gctaattttt ccaagtaaag agtctcgacg ccttcatttt gcaatgtctt
    11821 cgcaaagttg tcatgttctt cttgagcaat cggaagatat ggaatatcat cgaacaacag
    11881 acgatccata atatctggtg tcaaattttc cacttcttgt ccaggccgtt tcagtaaaac
    11941 tgtctttaat ttccctattt cagagaaaac gtgaataggt ttatccatgt tgtgttgcct
    12001 ccttcttttt gattacatgt ataggatatc ctccttttgt aatcactttc ataattttgt
    12061 ctcattcaaa atgagaca
//
LOCUS       DACCXW010000087        12068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCXW010000087 DACCXW010000000
VERSION     DACCXW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.088"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(277..1002)
                     /locus_tag="IVY71_002018"
     CDS             complement(277..1002)
                     /locus_tag="IVY71_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986065.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1156..1482)
                     /locus_tag="IVY71_002019"
     CDS             complement(1156..1482)
                     /locus_tag="IVY71_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986066.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1519..1884)
                     /locus_tag="IVY71_002020"
     CDS             complement(1519..1884)
                     /locus_tag="IVY71_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP7986067.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1921..2136)
                     /locus_tag="IVY71_002021"
     CDS             complement(1921..2136)
                     /locus_tag="IVY71_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986068.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2265..2600
                     /locus_tag="IVY71_002022"
     CDS             2265..2600
                     /locus_tag="IVY71_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986069.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2676..4673)
                     /locus_tag="IVY71_002023"
     CDS             complement(2676..4673)
                     /locus_tag="IVY71_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986070.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4771..5046)
                     /locus_tag="IVY71_002024"
     CDS             complement(4771..5046)
                     /locus_tag="IVY71_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986071.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5278..6012
                     /locus_tag="IVY71_002025"
     CDS             5278..6012
                     /locus_tag="IVY71_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986072.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            6051..7193
                     /locus_tag="IVY71_002026"
     CDS             6051..7193
                     /locus_tag="IVY71_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986073.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(7821..8306)
                     /locus_tag="IVY71_002027"
     CDS             complement(7821..8306)
                     /locus_tag="IVY71_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HAP7986074.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(8308..9072)
                     /locus_tag="IVY71_002028"
                     /pseudo
     CDS             complement(8308..9072)
                     /locus_tag="IVY71_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9242..10435)
                     /locus_tag="IVY71_002029"
     CDS             complement(9242..10435)
                     /locus_tag="IVY71_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986075.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(10519..10722)
                     /locus_tag="IVY71_002030"
     CDS             complement(10519..10722)
                     /locus_tag="IVY71_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7986076.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            10979..11221
                     /locus_tag="IVY71_002031"
     CDS             10979..11221
                     /locus_tag="IVY71_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986077.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(11143..11376)
                     /locus_tag="IVY71_002032"
     CDS             complement(11143..11376)
                     /locus_tag="IVY71_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986078.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(11383..11814)
                     /locus_tag="IVY71_002033"
     CDS             complement(11383..11814)
                     /locus_tag="IVY71_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7986079.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         3700 a         2471 c         1946 g         3951 t
ORIGIN      
        1 ttttttcata aaaaaacagt actgtctagt agtataaaat atacaaattt cttaaaaatt
       61 ttttcactaa aatcactacc tgaaacagtc taaaacactt atatatcaac gtttaatgaa
      121 actatctccg tttcatttca gatacaaata aatatagtcg ggctccaaat ccgaaaaaaa
      181 atacccccag tagcttatcc taagctactg gagatgtata agtacggtca tataacaacg
      241 gggtagggta aataaatgaa aaagatggaa gaatcattat tcaataactt ttttaaatac
      301 ttcgttgtaa tcagttacct gtccgttctt aaataccaca gaataatctt ttttcccatt
      361 ttcaagtaac cgaagtggaa tatctttacc aactttctct gtcactgcat tactgatacc
      421 tgttagatta gctaataaat ctgataaaga ttgagcatct gtagaaatct ctgtatcagt
      481 atttatcgtg attcctttat cttcatttac aagtttccaa gtaggtggta tagctgtgta
      541 tgtttctaca aatgaattaa gtttttcatt aagtgacttc tcttttatat ttccatctaa
      601 aaactctgta tatatggctt gagctatact aaaacaaagc tggccttgtt ctaataattt
      661 tacatcatta gttagtaaag ctgtgttcac gttatcaatc gcttcaagaa gagtatcttt
      721 ggtgtcccta acagctttag gtgtgccttc tggaatttcc ctacttgata cctcgtccat
      781 tttatcttcc ataaacccct taaaatcata tctttctccc aaatccatag aagtttttga
      841 gaaagctttc atttctgagt ctatttcttc aataatattt aacatgtctg atacatattg
      901 tttaggctga ttcgttttta atctatatgt ggtaaatatc tcaattcctc caattatcaa
      961 taaaattgcc aaacaaatcc ataataattt tttctttttc atttttcctc ctatacactg
     1021 ctgttataac agtgactaat gtaaagattt tcccagcaat catcagacta ttatttacag
     1081 ggaatcaaaa aaactcctta gtaagcttac acctttagga gttcctgttt gaacggtatg
     1141 cataatacat ttaaatcaat ttaacattaa cacctcagta tatggtatct ctacatatac
     1201 acggtctttt tttggatagt actcgacatt tatattgata gcacttccat tgtctaacgt
     1261 agctttagcc cggtacgtgt tatcaccatt atcatgagtg atcgtcacat catcacatgt
     1321 ccgatttaag ttatactgtt cttgtaaaat ctgagttacc aatggtacag atgacttctc
     1381 tagtccagat gaacccgtca ttcttgaaaa aattatacct ataatagcca atacaacgat
     1441 acctataatg acaaacttct tattcatatt tactttattc aactttttat ctcctatctc
     1501 caaaatgatt ttctctcatc agtaactcat tgacatcccc aatagtacac caaagatggc
     1561 tccagctacc cctgctatca tcaaaatcgt acccatttgt tcatctttct ctcgatataa
     1621 gtaacccata ataacagcaa cagcaccaaa taccacagga ataaatgcta atgatactac
     1681 catagaaatc caaccaataa tcaaatacag cttccctgtc tcttgtccat gtacgatttg
     1741 ttccttcatt tcattaattg tctgctcagt acttttcttt actgtgtctg ttccggttgt
     1801 ctcatctgtt tcttgtaatg ctccgcaaaa ctggcaatac ttactttcat ttgttatttc
     1861 tttaccacat tttctacaat acatgtcttc tcctcctaaa atcattcaat tgcttattag
     1921 ctaactgttt actttattaa atacatcaga aaacagctca tttattggta cacttaatat
     1981 ctcagaaagc attttcattt gatagggttt aggaatcgtt ctccctgttt gccacgaact
     2041 tacacattgc tgtgtaacat tcattttttt ggctaactca gtttgggaaa gtcctttttc
     2101 ttttctgtat cggctgagat tacttacttt tttcatgatt ctcccctcct gttttattat
     2161 atacaacaat atgatgtatt tcaactcata aaatatataa atacttcaaa tgtcattaca
     2221 aatacaacaa cttgttgtac tctactgaga aggggggtgt attaatgaaa cagaagcaat
     2281 atgcaaaggt cattagagaa aagcgaaaag cactcgggtg gacacaaaaa gaattagcta
     2341 aaaaaatctt ctctacacaa caggcagtag caagatggga aaattctgta acagaaccta
     2401 acctcgatag cttaacagct ctttctagag ccctaggaac ccctgtaagc cattttctag
     2461 acaatgttga catagattat gaggaagagt ttttggctct ctatcgctct cttagtactg
     2521 aagatgcaat tcgaacaatt gactatatga agctattaaa aaggcaagaa aatgaacgca
     2581 atcagctatt gaaagattga aaaataaata acccaccgaa ctttaacggt gggtgtattg
     2641 agatgaaata agattaagag ctatggataa cctgtctagt ttaatataat tgggtctctt
     2701 ctattcctta ctctccgtcc atcatctctt gttacacttg taaattgata aataggaact
     2761 aattcaactc ctgataatat caattctttt attacttcat ctatccctgc ccctttcgct
     2821 agatatacta aaaaactact gcttgctttc acattgtata gtttcttaat atccttagaa
     2881 gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc tgttttctat taaagcattt
     2941 ttcacgctgt tatccctgac atttcgtagg cgttcacaaa cagtgttaat aagttctttt
     3001 tctgtcatta gatatgtccc ttgccacgat aagccctgta aaacttcatg tgcttcatct
     3061 aaaatcaatt gtttttcctt gctaagttct gatcgggaat tttgaggaaa tactctttta
     3121 gcctcttttt cgttaaaaca tatcttaacc cattcataag aaaaatattg atatgtaaaa
     3181 tgaaattttt ccagaagtgg aaggatatct tcattcaatt tttgaatcac agaagttgca
     3241 ttaaaaacgt ctaatcgata gacattgctt ctcttatgtt taaagtttcc taacgctttc
     3301 tcttccttta acctatttag gtattcgagc atcttcaaag gaacatcttc atcatctaat
     3361 ttttttatca atcctataaa agtcataagt gatagtaact tattacattt atctatgtca
     3421 taagttttac catagtaaga actcattttt ttagctaatt gttcccccga caaaatacaa
     3481 gtcacacttt ccttactatc atcaaaaaaa ttaacatcag aaagcaagac atcatagaca
     3541 cgtcgcattt cctctagata attccttatt gtcctgaaaa gtttgtcttt cattgattca
     3601 agagtgtcaa aaagtacttt tgattcctct ttcaattttc gaaaatgtgt aggatacaag
     3661 tctaggttct ctaatagcca attaaatgtt tctacccgac tgtctttctt taaaattagc
     3721 atcacaactc caataaaatt ttcactgata tcacaattac tggaatgaca ataaaaaatg
     3781 gaatgcccag ttttactggt aaaaatacta gcagagggat tatgatcgtt gtgaaacaga
     3841 catcttaagt ttttacttga cttaagtaat ttattcatat caatggttaa caatctttca
     3901 taaatctcat taaaagacat gtcggaggaa tcaaacaggc agtctttaaa ccactgtttc
     3961 atttcttctt tgtcgtcatt ttttatcaac ttaacaaaat cggtttcatt gactagttca
     4021 tactctctcc ctgaatcact ttgacaattt ttagaacatg tcatggtaaa atcaatcgga
     4081 agctcagtaa tatctaacat gttgtcttca ttaattatta tcactgcatc tgacttatat
     4141 cctccaaaaa acattctatt cgaagcattt acattgtcat cacacccggg gatgttcttt
     4201 ttcaagtatt cgtaaacagc aaccatttta ccatagtcag taattgcttc ttttaataga
     4261 aagacaatct tgtatttttc tagattttca gagtaccgaa tacttttttg tatcgcaaac
     4321 gcagaattat gaactaaatt gatagataaa accttttcta tgcttaagta tctactatca
     4381 tctttactca aatctactac cttccccttc tcatctttaa cagtgttatc gacatccaga
     4441 ataattactc tggtttccac aaaactctcc ttgtcattgc ctttattctt tgtcttgctt
     4501 ggaataaatg attgtgatga atttagtatc tctacaaact catctcttgt aacttctcta
     4561 tagttatcta aacacctatc tcttacatca gttttttctt tttttgtaac tggcttctct
     4621 aataattttt ctttatctac ataaattgga atattaatca ttgatgtttc catttgtgtt
     4681 tcctccttat aaaattctgt taaagactaa aacatacaga taataaaaac aaactcttta
     4741 ttactatatg ttttagtctt atttgtttac tcaaccttta taccgttcca tgaaactatc
     4801 aatatcagat tttttaaatc taatgaccgt atcaattttt ataacaggta acccgtattg
     4861 agaaacatat tttctaatcg ttcctactga tacacctaga tattcacttg cctgctgata
     4921 gtccatatac tctagagtga ctccagaaat aatgtcttct agcataggct ttagcactgt
     4981 aattatctct ttcacttgtt gttcaacttg tatagaaaca atgctatcaa tgatttgtag
     5041 agacatattt ccccctcctt tcgtgattgc ttgaacaaat tttctaagaa ttatcttcgt
     5101 attttttgct caaaataaaa gtattttgtt attaaccgat taatcaaaca aaaaatgatt
     5161 accagttcat cgacatacac ataatacctc gataaaaaag gggtgtcaat agttttgtaa
     5221 aatttattta caacacccct tttttgaggt atactaattt taggaggtgt ggtcaatatg
     5281 ataaaacgtc caccaataaa ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa
     5341 gctataagaa agagtaaaaa attaacacaa ccagcatttg gtcttatgat taacaacggt
     5401 caattaattg acaaaaagac tatctacgaa tgggagaagg gaacctattt accaatacca
     5461 gagcgattat cccgaattgc tgatttggga aatatgagta tagaggagtt agtttgtggc
     5521 aatgtagaag aatatatttt gggaattatt ctttatagag atagcattgt actagatggt
     5581 ataacatttc cagataaaaa cctgttccag catcttcgtc aacagttccc accagtacac
     5641 tctaatttag atacatggct ggatagatat tccaaattag aaccagagat gcaagaattt
     5701 attgctaaca agacttgtaa taaagttaaa aatgaaaaaa ttagtctttt caatatctta
     5761 aaaatagaag aattattcat caatgctatt gtagaagaat ttgacaataa cattcttttc
     5821 ctcactagca gtatagaaga actactagaa agaatggtag atgaatggct acctattcaa
     5881 ttgaaagaca tgtcttatcc cgaagaagct gtgagagaaa ttactgataa tataaacaag
     5941 ctagaacaaa cgatttcatc aattgggaaa aaatacacta agaaaaaaat gaagggaggt
     6001 gataccatct agtggtaagt actccttgta aactaagagg agatgatttc atggcaaaaa
     6061 tatcaaagta caaaaagaaa aacggtcaag tggcgtggat gtataaagga cacattgcca
     6121 ccgatccacg aactggtgaa aaaataaata ctacgagaag aggttttagt agcaaacaag
     6181 aagctcaaaa agattatgac gagtacagac atcaaatatt atacggtgtt aaaaagaaag
     6241 cacctgatat gacatttgaa gacctgtata atgaatggat aactcatcag cgtaccagtg
     6301 ttaaagcttc aacaatcgca atctcagtaa gatacgcaaa taatcaaata cttccagcat
     6361 ttggtaagct taaactatcg aatatctctg tcccatattg tcaaaaagtg gtagatgaat
     6421 ggcacagtaa atatgaaagt tacgattata tgagaaaaca gactgcacaa attttacgct
     6481 acggggttgc aatgcaatat attgacaata atcctatgga aaaaacactt cttccacgaa
     6541 aaaaagagta tgagaaaaat cgtaaatttt actcaaaaga ggagcttaat aatttactgg
     6601 atgcttttaa agattttggt aacatgaaac aatacgcctt cttcagatta ttggcataca
     6661 caggtatgag aaagagtgaa gtactcgctt tacagtggaa agacatagac acgtttaata
     6721 aagaactaca tgtcaataaa actcttgctg tagatgaatt tggtaaagtt ataattcaat
     6781 ctccgaaaac aagagcttct agacgagtta tctctttaga tactgagact ctttctatcc
     6841 tcaacaactg gaaattacag caaaaagaag agtatttaaa acttggatac aatactagct
     6901 ctaaagagca acatgttttt acaacagtta aaaacactct atacattcca aacactgtta
     6961 atgattggtt aagatatatt ttgaaaaaat ataacttgcc tcgtattaca cctcatgggt
     7021 ttagacatac acatgctagt ttattattag aagcaggaga atctgtcaag gtagttcaac
     7081 aacgattagg gcatgaaaat agtaaagtga cattagacat ctatgcacat attacaaata
     7141 atgcacctaa gaaaacaggt caagactttg ctgatatgat ggctcatcag taaaagagct
     7201 ggtcaaaatc tggtcatttc tagtatacag gcaaaacaaa aaagctagaa cccttatata
     7261 tcaagggttc tagctcctat aacaggttta cccaacactg ccttccaatg actcttttgg
     7321 gaatcagagg aaagaatcag gctttataga gatatttcat ctaacaaatt actttagact
     7381 gacagtatat atcagcaggt actgtcatcg ggaacgtagc caatatcgta tccaaaacag
     7441 caggtagaga agctatttga ggattcaagt tattctattt caaccatgaa gttaagttca
     7501 ttaaaaataa agatagttgg tatttcaata atttttgtat agagttaatc actttattga
     7561 tgctaattca tcttgattgg tattataatc gtattcaagt tgcgtggcgt gcttttgcta
     7621 gaaaatatat aactgttctt tataaagaaa tataaggatt aaacattttt tatcatagag
     7681 ttttacttat ctatagaatt atgtctgttc ctaattcagt ttgatgaatt aactgataaa
     7741 aaaatatcta ctatttaaag atacagggat aagtaagtaa aaaaataaca tgtcattaga
     7801 tagtagacag ttccaactct ttatttacct tttttgtatg ctgcactgtc aatccaagtt
     7861 cctaaattgt tgtatccatc atcacttata taatcatatt ggccatcaat aatttcgtca
     7921 aaccacaaat attctccatc tggtttttgc ccaattattt taattactga ttgactagta
     7981 gttgtcatct ggttaaaatc ttttatttta cctatattaa cggccaaaat agcatttcct
     8041 cgcttataac tctcctggat ttcatattta acatattctc gatcaagagt ttctttacct
     8101 atcagcacca cagtcactga tgttccaatt aattgattat ctatccagtt ttttatagca
     8161 atatctccat tacgtttaac tttctcaaat tcagccttat ctacaaaacc agcttccttt
     8221 gttcctcgca aagtccagct gtttctaact accattgctc ttgttacatc tgcataatgg
     8281 aaactaaaaa aaacccttct caccatatta atcctcctga atactattta ttaaactgac
     8341 aatttgttca acttttttat ctacttctcc ttcagtatct aatttttcta gcgcttgaaa
     8401 tttacttttt aaggaatcat caaaataatc actgaaattc tcttgaatct tatcaaacaa
     8461 aaccttactt gcacctttgg taaatccaat cggaactact atattttcat tttgaacaga
     8521 taattcaaat tcttcaatca ttccatctgc tgtaactatt tcacctgatt caagtttgtt
     8581 tccaaaaaca aaaatactaa tcccagactc tgcaataatg ttttctcgat atcttctcca
     8641 ctcatgaggc atatcaaggg gaagtggtct taaagaaagt acgtcttgta tgttattttt
     8701 tcttaaattt cttctctgct ttagaactcc tgaaacaata ctattaccaa ttccaagtcc
     8761 gtaacctgat actattctat aaccggattc agaaagccta tatgctaatt tctcgacaaa
     8821 ttttaatcct ttcgattcag atgtaaatgg agtataatca acagcacttc ccgaaataaa
     8881 aatagttttt ctcaaatact tttttctaat acttttcaaa atatcagtaa tctcagaata
     8941 atcatctact tcaacaacgt ttatcgaaaa tctggacaaa tcattgattt gtagtcttct
     9001 ttttgtcagt tcataattat atctctcttg atcaaaaaaa tcctcagaat cgacaaaatt
     9061 ttcctttaaa attatatggg attttagatt atctttttag aagttcttga atgtcggtat
     9121 agcccgtgtt tccgggcttt tccggcattt ttgatattgt tagatactta caaatgttct
     9181 tattacctct caccatttcc ccctcaaaag tagtaaatgg agtagtaaaa ccggccttgt
     9241 gtcaggctgc cagccgttcc atttctgccc ttgcggaatc gaatgtggcg tgtgcataat
     9301 agttcagcgt catggtgata ttggaatgtc ccataatgta ctgcaaggct ttggggttca
     9361 ttcccgcatt ggctaaccgg gtgcagaatg tatgacgcat agcatggggc gtcgtcgttt
     9421 tcggcaacgc ttcttcatgg ctcttgttgt acttcttcac aagcctgcca aacatcccgc
     9481 cataatctac cgccgtcatg ggatagccgt tctgtttgag aaacaggaaa ttggcatacc
     9541 cgtcaataat gaacggcttt gcacctttgc ggttttttat cactcgctgg aacgcctgat
     9601 acacttcctc attcatgggg atttttctga cgccgctttg ggtcttgggt tcgtcaatat
     9661 agtaaccgac gccggtattt ctcaaaagct ggtggctcac gatgattacc cggttttcaa
     9721 aatccaaatc tttgtccgtc agtccgcaca gttccgaaat gcgaagccct gtccccagca
     9781 gaataacgat tgcgtcataa tgcttttggt aaaccttatc accttgaata aaggacagaa
     9841 aactttcttc ctgtgctggt gtaagaggca cctttggttc cgtatcgtcg tccagaacgt
     9901 cactaagctg gaagtcaaac gggttttttc ggatgcaatc gtcctgtatg gcggtgtaaa
     9961 aagccgcttt cagggaacgc ttgtcgttat tgatggtctt ataagaaagg cccttttctt
    10021 tcatccgaag cgcccattcc tttgcgtcgg acagcttcac actgtcaatc gggcagctcc
    10081 ccagccgatc ttgttccaga atacgcatca gccgttcccg gcccagtttg gtgttgtgct
    10141 tcacattgcc ccggtgcctg atctgcttgg cgtagagctg gcagacggtc attttcttgc
    10201 cgatggtgtc tatcccgtcg tcaaggtctt tctggatttc tttctccttt tcccggagtg
    10261 aaatatcctc ccgtttacct gccggggtct tatccgtagg cactaacttc catgagtaaa
    10321 caaactgcgg ttttccaaag gtatcaacat atttgtaagc atatcgtccg tcttttctct
    10381 ggctctctcc agaccgcaaa acgcgatttt tgctgtctcg tcttttttct gacatctcgt
    10441 catgctcctt tccatgacgg aaagagccgc atatatgctt ataaattatt ataacatatc
    10501 tgcggccctt tttcactatt agattgtgtc caaagtgtca attattttct caaactgctt
    10561 gcgtttgatc tggatgcggt tgccattcat aatgacccag ccagaggccg gattttcctc
    10621 ggccagctta cgcaatttgt tttctccgat acggaaatat ttagacgctt cctcaatcgt
    10681 cagcgtgtat ttttcccaaa taggcacatc atggttcata atgcagcccc ctttcacaaa
    10741 ttcaaaggtt tgtgtgattt aggggcatag acggacggct gctggcacag ctccacggga
    10801 ctttcacccc cgcgtggttc tcacgcggcc ctacccattg cctgcgacgc ttttaacgct
    10861 cggactgtgg ctataaggga gtatcatagt cctgcctgta cgtcgtcgcc cgcaagccgc
    10921 cacgctcaca ttgcggcgtg gtgttcctcg ctccgctttt cccgtaggga aaagtatcat
    10981 ggcgcaccag ccgtccggct cggcacagac agaatgtgtc cgtatgccct atgatgcgcc
    11041 gccgttatct tgcgggcata tttatcaagg tgcgatcagg ccaccgggaa aacaccgaaa
    11101 cggggaaagg gtaagccgtt tcatgctgcc ggggccggtt cgtcagttcg tgacgatagc
    11161 gtggatgagc ttgatttcca gacggcgctt catggcctcg tccagacaca catgcggata
    11221 gccgttttcg tcatagagcg tccgggtaca cagcttgttc atgtaaccgc tgtaatactg
    11281 caaaacgcgc tctacggctc cggcatcccc agcgcgggcg gcctcgatca ccgaaagggg
    11341 caaaagctca atgcccttgt agttcgggtt cttcatccgc gtctaacctc ccttcggcat
    11401 caacgccttt agctgtttcc gcagctccga taaggtattt gtcctgtgcc gctgaacggc
    11461 actccgggac attcccacaa ggccgccgat ctcaccatct gccatatcca gaacacagtg
    11521 caggattaaa atactctgct ccatgctggg cagcgcggca aaggcttcgg caacaagctc
    11581 attgttgata tgtaggtcat agccatgcga cgaaaacgta aagctgtcgc tggggtagtg
    11641 atctactgta cagagcttgt ccaaatctgc ctgtgacaaa gagcttagtg acacttcacg
    11701 gtcacggcgg cgcttcattt cagccaagta gcttttggcc tcgttacgca gtaccgtctt
    11761 gcaaaaagca tcgaacatgc accgcgcttc atagtcgcgg gggatagcat ccatcttctc
    11821 accccctttc tgtgggggac ttaggtggtt ctctcccttt ccgctaacaa gactttgaca
    11881 aggccgccag ctacccgcta aatacctcct ttttgaaaat ttctcaaaaa atttttttgg
    11941 ggacagccgg gccgcaaaag acgacaaaaa tcgcccggca ggtcgcaaac ctaccgggcg
    12001 agaaaatgct ttgctatgct gctgatttac atggtatagt acatactcat aaccggaata
    12061 gccctgtg
//
LOCUS       DACCXW010000088        11958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCXW010000088 DACCXW010000000
VERSION     DACCXW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.089"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            46..2064
                     /locus_tag="IVY71_002034"
     CDS             46..2064
                     /locus_tag="IVY71_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7986080.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            2491..4905
                     /locus_tag="IVY71_002035"
     CDS             2491..4905
                     /locus_tag="IVY71_002035"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7986081.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5253..6806
                     /locus_tag="IVY71_002036"
     CDS             5253..6806
                     /locus_tag="IVY71_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7986082.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7187..8971)
                     /locus_tag="IVY71_002037"
     CDS             complement(7187..8971)
                     /locus_tag="IVY71_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986083.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8986..10707)
                     /locus_tag="IVY71_002038"
     CDS             complement(8986..10707)
                     /locus_tag="IVY71_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986084.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10807..11568
                     /locus_tag="IVY71_002039"
     CDS             10807..11568
                     /locus_tag="IVY71_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7986085.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3918 a         2161 c         2425 g         3454 t
ORIGIN      
        1 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
       61 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
      121 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
      181 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
      241 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
      301 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
      361 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
      421 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
      481 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
      541 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
      601 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
      661 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
      721 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
      781 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
      841 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
      901 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
      961 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
     1021 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
     1081 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
     1141 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
     1201 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
     1261 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
     1321 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
     1381 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
     1441 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
     1501 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
     1561 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
     1621 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
     1681 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
     1741 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
     1801 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
     1861 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
     1921 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
     1981 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
     2041 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
     2101 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
     2161 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
     2221 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
     2281 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
     2341 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
     2401 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
     2461 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
     2521 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
     2581 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
     2641 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
     2701 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
     2761 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
     2821 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
     2881 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
     2941 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
     3001 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
     3061 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
     3121 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
     3181 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
     3241 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
     3301 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
     3361 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
     3421 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
     3481 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
     3541 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
     3601 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
     3661 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
     3721 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
     3781 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
     3841 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
     3901 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
     3961 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
     4021 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
     4081 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
     4141 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
     4201 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
     4261 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
     4321 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
     4381 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
     4441 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
     4501 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
     4561 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
     4621 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
     4681 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
     4741 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
     4801 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
     4861 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
     4921 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
     4981 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
     5041 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
     5101 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
     5161 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
     5221 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
     5281 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
     5341 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
     5401 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
     5461 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
     5521 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
     5581 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
     5641 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
     5701 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
     5761 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
     5821 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
     5881 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
     5941 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
     6001 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
     6061 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
     6121 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
     6181 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
     6241 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
     6301 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
     6361 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
     6421 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
     6481 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
     6541 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
     6601 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
     6661 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
     6721 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
     6781 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
     6841 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
     6901 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
     6961 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
     7021 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
     7081 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
     7141 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
     7201 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
     7261 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
     7321 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
     7381 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
     7441 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
     7501 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
     7561 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
     7621 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
     7681 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
     7741 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
     7801 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
     7861 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
     7921 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
     7981 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
     8041 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
     8101 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
     8161 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
     8221 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
     8281 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
     8341 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
     8401 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
     8461 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
     8521 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
     8581 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
     8641 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
     8701 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
     8761 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
     8821 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
     8881 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
     8941 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
     9001 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
     9061 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
     9121 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
     9181 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
     9241 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
     9301 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
     9361 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
     9421 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
     9481 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
     9541 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
     9601 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
     9661 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
     9721 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
     9781 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
     9841 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
     9901 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
     9961 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    10021 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    10081 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    10141 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    10201 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    10261 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    10321 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    10381 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    10441 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    10501 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    10561 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    10621 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    10681 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    10741 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    10801 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    10861 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    10921 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    10981 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    11041 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    11101 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    11161 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    11221 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    11281 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    11341 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    11401 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    11461 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    11521 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    11581 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    11641 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    11701 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    11761 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    11821 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    11881 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    11941 tatgttaata aggcaaaa
//
LOCUS       DACCXW010000089        11878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCXW010000089 DACCXW010000000
VERSION     DACCXW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.090"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            366..635
                     /gene="rpsN"
                     /locus_tag="IVY71_002040"
     CDS             366..635
                     /gene="rpsN"
                     /locus_tag="IVY71_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7986086.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            650..829
                     /gene="rpmF"
                     /locus_tag="IVY71_002041"
     CDS             650..829
                     /gene="rpmF"
                     /locus_tag="IVY71_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7986087.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            861..1238
                     /locus_tag="IVY71_002042"
     CDS             861..1238
                     /locus_tag="IVY71_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986088.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1259..1408
                     /gene="rpmG"
                     /locus_tag="IVY71_002043"
     CDS             1259..1408
                     /gene="rpmG"
                     /locus_tag="IVY71_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7986089.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1432..2415
                     /locus_tag="IVY71_002044"
     CDS             1432..2415
                     /locus_tag="IVY71_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7986090.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2445..2567
                     /locus_tag="IVY71_002045"
     CDS             2445..2567
                     /locus_tag="IVY71_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7986091.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2585..4108
                     /locus_tag="IVY71_002046"
     CDS             2585..4108
                     /locus_tag="IVY71_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7986092.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4132..4371
                     /locus_tag="IVY71_002047"
     CDS             4132..4371
                     /locus_tag="IVY71_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986093.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4564..5025)
                     /locus_tag="IVY71_002048"
     CDS             complement(4564..5025)
                     /locus_tag="IVY71_002048"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7986094.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5220..5438)
                     /locus_tag="IVY71_002049"
     CDS             complement(5220..5438)
                     /locus_tag="IVY71_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986095.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5556..7172
                     /locus_tag="IVY71_002050"
     CDS             5556..7172
                     /locus_tag="IVY71_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7986096.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7413..8753)
                     /locus_tag="IVY71_002051"
                     /pseudo
     CDS             complement(7413..8753)
                     /locus_tag="IVY71_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9029..9184)
                     /locus_tag="IVY71_002052"
     CDS             complement(9029..9184)
                     /locus_tag="IVY71_002052"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7986097.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9480..9812)
                     /locus_tag="IVY71_002053"
     CDS             complement(9480..9812)
                     /locus_tag="IVY71_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7986098.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10326..10517)
                     /locus_tag="IVY71_002054"
     CDS             complement(10326..10517)
                     /locus_tag="IVY71_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986099.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10611..10997)
                     /locus_tag="IVY71_002055"
     CDS             complement(10611..10997)
                     /locus_tag="IVY71_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7986100.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            11691..>11878
                     /locus_tag="IVY71_002056"
     CDS             11691..>11878
                     /locus_tag="IVY71_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7986101.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFT"
BASE COUNT         3973 a         1843 c         2262 g         3800 t
ORIGIN      
        1 taatcaaagt tttaaaaaat tggcgataaa ccagttaaac ataaaagatt gaataaaaag
       61 ttatagtttc tttcatattg ccgttttaag tagaggaaaa gctacttaaa acggtatttg
      121 tgtttgattg gactaataaa agtgaattct tatttttcag agcctaaact gcatattctt
      181 ttaaatgatt tgattatctc tatgtaccga taaattcgtt ttcaaatagt tatctattca
      241 tatgagtttt atttcatgtt atttattgtt gacaagagca actcttgttg gtaaagtatc
      301 agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat caaaataaaa ggagtataaa
      361 ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa acaaatggcg atgattgaaa
      421 agtatgcaga taaacgtcag gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat
      481 tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa
      541 gagggtatat gcgaaagttc ggtatgtccc gtattaagtt tagagaactt gcccatcaag
      601 ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc
      661 agcaagaaaa acatctaagg cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa
      721 accagaaatt tcatttgacg aagcgaatgg tgattataga cgtagccatc atgtatcttt
      781 aaaagggtac tataatggaa agaaagtaat taaaggagct tcaaaatagt ttaatatata
      841 acttttagga gggaatcaaa atggaaatta tttacccacc tttagttgaa gaaggattaa
      901 agtattatct tgaaacgacc cagcaatcgc tagataaatg cactttttat cgttcgatgg
      961 tagagagagg aattattact gaaacgggtt taccgaccca acaggcaatt gaaaatggat
     1021 tagtcaaaga ttactatgag gaccaaggct tatcatttga tgatttttta agaatctatc
     1081 caatttttga agagtatgac gaggagttat ttcaatgtat cgatggttat tgggagatac
     1141 ctgttgatat gaaagaaaat ttagtttcac aattggaatc tggcgagtta acttttgaag
     1201 atgcacaaca aattcaagca tatcttgaag atcgataagt attgaaggag gaagatttat
     1261 gcgtcaaaat attattttgg aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa
     1321 aaataaacgg aataaccctg aacggttaga attaaaaaag tattcgccaa aattgcgcag
     1381 acgggcaatt tttaaagaag tgaaatagct aagtggtaaa aaggtgacaa aatgggcata
     1441 ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa caacgctaat caatcaagcc
     1501 ttgcaagtct caccctaccc cagggaagaa attattataa ttgaaaatga gtttggtgaa
     1561 gttgggattg atcacaaatt attagttcat agcgaggaac gggtgttaca actaaataat
     1621 ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa tattgatttc tttactggaa
     1681 acaatagaag aacatcaaca accaatttca caaatcatca ttgaaactac aggtatagca
     1741 gatccccaac caatcattca aacactttta acagcgcctc aactaagagg aagagtatat
     1801 atcgatagtt tattgactgt tattgatagt gacagttttg aacttattga aagagaacca
     1861 caagccctta agcaacttgt catggcagat cgcatttttg tgtctagaaa acaaaacttg
     1921 aatttatctg cactaaaaaa attacagaga gaaatcaaaa gtattaaccc tttatccgat
     1981 attcgaatgt tcacacatag tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa
     2041 tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa atcactataa tggcaaatac
     2101 tatcatcaac accatcaagt acatgagttt aaatcaattc tcttaacttc agatcaagac
     2161 ataaaaaaag attttttact ccaatggatt gactggctga ttttaaccaa ccaagggagc
     2221 ctgtatcgtt ttaaaggatt gattgcttta caagagcagg attttatagt tgccatgcaa
     2281 gaggtaaacg aacaggttaa ttttcaatac accactcgac aaaaagacca aaccactatt
     2341 attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac agagttttga taaattactg
     2401 gcggaaagca aataatgata agtagtgatt gggggtaatt tctaatggag aaaacggata
     2461 tatctagtgc atatcgacga cttaaaagcc cgaatattaa aactagaaaa agggcattaa
     2521 aaattatcaa agaacataaa cgaaataaaa tgaagaaatt agcataatta aggaaggaaa
     2581 aagaatgaag aaaatcagtg taggacttat tgggatagca gctctaggat tattgggagc
     2641 ttgctccagc actaatgatg caaaagtatc gaatgataaa gatggaaaat tagaaattgt
     2701 aacaacattc tatccaatgt atgatttcac caaaaacatc gtcggcgatg aagcgaacgt
     2761 ggatttaatg gttccagcgg gatcagaacc tcatgactat gagccctcag ctaaggatat
     2821 ggcaaaagct catgatgctg atgtttttgt atatcataac gaaaacatgg aatcatgggt
     2881 accaaaagct aaagaaagtt ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga
     2941 catgatttta ttgccaggta gtgaagaaga agatcatgat cacggagaag aagatcacca
     3001 tcatgaacta gatcctcata cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa
     3061 tatcaaggat cagttagtta aattgtatcc gaaaaaagca aaagtatttg agacaaatgc
     3121 tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac tataccacta gtcttaaaga
     3181 agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt ggatatttag ctttggatta
     3241 tggattgaaa caagttccta ttgctggact aagtcctgaa gaagagccat cttctggtcg
     3301 attagctgaa ttaaaagagt atgttaaaaa aaataagatc aattatattt actttgaaaa
     3361 gaatgctaat gataaaattg caaaaacttt ggccaacgaa gctgggataa aattggaagt
     3421 actgaatcct cttgaaagtc ttacaaaaga acaaatggat aatggtgaag attatgtttc
     3481 agtaatggaa gacaatctaa aagcgttaga aaaaacaact atggttgctg gtgaagaggt
     3541 agttccagaa aaagaagcga aggatgaaaa gacagttgct aatgggtact ttaaagatgc
     3601 cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa tggcaatctg tttatccgtt
     3661 gttaaaagat ggaattttgg atgaagtttt cgattacaaa gcaaaaatca ataaagacat
     3721 gacagctgcc gagtacaaag attattatac tactggctat aagacagata ttgatactat
     3781 caacattaaa gacaacacca ttgattttgt agtcaacggt gaacatcatc aatacacata
     3841 caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa
     3901 ttttgaaact gatgatgcag gtgctggtcg ttttaaatat attcaattta gtgatcatgg
     3961 aattgcacca agtaaggcag agcatttcca tattttcttt ggtggagaaa gtcaagaaaa
     4021 actctataat gaattgcata attggcctac tttctatcca gcttcattgt ctaaacatga
     4081 aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt ggaggaaata catggtaaga
     4141 aaagtattat tgtctatgga ttccaagaat attgatgctt atcggcgtta tcgatttgca
     4201 aagcaaatga gattaaatgg gagtacatta gctgatattg ctgcaacttt acatgttagt
     4261 attgacacag ttcatagagt attaaaacgg gatcctgatt gttgttttat caaggtcaat
     4321 actggaagaa gtagacgttt agcaaaacgg cataatagct tggccaatta attgataata
     4381 ccagagtaga tcatttttcc cttatttgga tttgatctgc tttttattat gtcttgaatt
     4441 gtcaaatcaa gcgcaacact ttatcaaaga agacttcata aggtgttctc caatctaaac
     4501 actttctagg tctgttattc agtttttcaa tcatctgatt tatcgttgag ttatctaccg
     4561 tagctatctc tttcgatttt ggcaagtatt ctcgaatgag tctattcgta ttttaatttg
     4621 ttcattgttg ccaagggcat gaggatctgg aaaataaaag ggcatattat tcaatgcttc
     4681 tgtaactgcg aaatgattgg agaattcttt tcctctatct ggagtgatcg ttttgaccgc
     4741 ctcatttgga atactgctta aaagttcaat cattttctcg ctcacataag cggctttctt
     4801 tctggggacc ttctttcctg ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa
     4861 acaagtacca ccagttttgc cagctacaat atcggctttc caatgaccaa aatcacttct
     4921 atcatctgcg gattttggac ggccatggat cgaatgagta aggcaaatct ttccacgaga
     4981 ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc
     5041 tttattgccg tgtgagaggg gttctgtatc gaaaagatca ttgcaaattg cacgttagat
     5101 cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc aaacgattcg agatttcctc
     5161 tggcgaccac tgaaaatcga caaataaatg acgaacaatt gaccaaactc acggatcatt
     5221 tagaatgtga taacgtccgc atttttttct tcgataagca taattttttt ccgctttgga
     5281 aggtgaatag tttttttctt gtttcttctc agttcatgta aaatagtaaa cgggctatgc
     5341 tgaatgtcgt tgccaattat actaatagaa aatccttctt cgtgaagcag aaagattctt
     5401 tctcgttcga gtatagtaag atgacgatac tggctcataa ttattttcct tctaggagtg
     5461 ttctcgacaa acttatacta gttggatatc gattatgact catttttatg tgttgcactt
     5521 aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa aaaaatacct attacaatct
     5581 taactggcta tcttggttca ggaaaaacga tacttctaaa tcatattctg tacggtgatc
     5641 acggaaagaa aattgctgtc attgtaaatg acatagggga agtaaatgtt gatggggtat
     5701 tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact tgtttcgatg ccgaatggat
     5761 gcatttgttg tacattacgc gaagatttga ttgttgaact agatcaatta gtgcaatttt
     5821 ctgacttgaa tcaaattgtt attgaagctt caggaatcag caaaccaatt ccaattattc
     5881 aagcaattat attagctaaa aaccctttag acattgattt gacccaacga ttagagattg
     5941 gtgccattat tatagtagtg gatgtaaaac gtttttggga taatttcgaa acgggtgaaa
     6001 agattcagga aagaaaagct gaaggtaatg tagttgaaga agaacaggat attcgtggct
     6061 tgttaattga tcaattcgag tgttgtaaca tattactttt aaataatagt gacttagtgt
     6121 caaccaagac gatttacaaa attaacggtc taattgctaa gttacagtcc aatgccaaaa
     6181 taattaggac aattaaaggt gaagtagact tatcgaaaat tctaaataca aatcttttta
     6241 atatagatga gacttcctat tcagtgggct ggttcaaaga gttagagctt ggctatcaaa
     6301 atcatattcc tgaaacagaa gaatatggta tctcaagttt tatctatcga aatcggttac
     6361 tccttgacgc acaaaaattt gcccatctta cggataaaaa ttttcctggc aatattacaa
     6421 gagcaaaagg gtttctttgg tttgcccaac atagtgattt ttcgacgatc tttgaaacat
     6481 cgggtagcta taaaagtatt gaatcattaa attattgggt agctagttta cctaaaattg
     6541 agtgtcaaat gattatacaa aaaaatcccg aattgaagga agcctggcat ccgttgtttg
     6601 gtgaacgcat taataaacta gtttttattg gtatgaagat ggatcaacaa tgtattaagg
     6661 aacaacttga gggttgttta actgattctc ttaatgcaga taatttccga aaaatttcac
     6721 catttccatg gaaaaagtct aatatgttta gccagatatc gttaatgaaa tttttaagaa
     6781 aagctggtgt caagataggc gatgtcataa ttattggcat tttgattatt acctctttta
     6841 ttcccttatt tgtattctct atagaaaagg agaatgaaac aactgaagat gctatcaaat
     6901 atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt cgatttggat aagatcaatc
     6961 ataaactcgt gaattatcat cctgcgaagg gacaatataa tattgttgag attaaagatg
     7021 gaagaattcg agttaaagaa gataatagtc ctgatcaaat cgcggttaga actggatgga
     7081 tctcaaaacc cggtcaaacg agtatttgtt taccccataa gttagttatt agtattgaga
     7141 aaaaagaaag cacagattac tacatttact aatctggaaa tagatgagga ggtttttaat
     7201 aagaaaaaga tattattatt aataatagca ggggctttat taagtgtgtt aagcgcttgt
     7261 ggacaagaaa aagaagcggt gacaacaggc acaagtatct ttaacgggac ctgcaatggt
     7321 ttagttaaca gtcggttaga ttacttattt tggctcttcg tcaatgagtg ttggtgaaga
     7381 aattcaatag aataatcgag atactcacac ggctagacag ctatcttgtc tagccgtgtt
     7441 gttgttctgg ctttagtcag ttgggtagta ttcttagttt tcttaaaata ttttcctcga
     7501 tataaaaaga ttcgttctcg gtagttttga aagtttctat agccataggc aattcgtttt
     7561 agtactttga tatggttatt caaacattct atagggccgt tggaataggg aacgcgcaac
     7621 gctcgtttga tttctgtttg gtatttcttg aaggtcgtga acactggttg gtagtgctct
     7681 ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt caggaacatc ttgtcttctg
     7741 ataacggaaa gaaaatcctg gtagacttcg tagcctcgct ttaaggaatc atcataagat
     7801 aatagccgat cgactatctc tgcttctgtc agatgatctc gaaatgacgg acgccagtag
     7861 cgatgctgac ctttcaattc ccacgcattc ttttgaagta gtttccagta tttctttaag
     7921 tgcttcgacc gacgatcccc ttttttaaag gtcttcatta cttgaattcg gtggttggta
     7981 aaagcccgac caatgtgttg gctaatatga aagcgatcgg cgacgattcg ggcattcgga
     8041 aaacactctt tcaccaatgt gcgatagggt gtatagaaat ctgaaaccac tagacgcact
     8101 tgttcacgga cttttatagg gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga
     8161 ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct
     8221 cccgagacat ttttcactga tttgaattca tcgaaacaca ccacttctgg taacagctgg
     8281 ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa
     8341 atgtgcttca tacgtgcgat caaagacata gaaagaggtt cgcttaatag ctcaagaatg
     8401 gcttgtttga ctctgcgagc aatggagcag cgacgatcac taactgaatc ttctgctaaa
     8461 aaagtctttc cacagtgttt acatttgaag cgctgtttgt taatccgcag aatggtcttg
     8521 tagttactga catcattcaa taagatggat acttttttcc agccctattt aatgatcgtt
     8581 tgttcatttt gatagtggcg atgaaagcat tcctccggtt gataggtcaa tgtgccatca
     8641 aaaacaaaac atgtttcgtc attgattcgc tctttgcgaa aggcatcttc atgaaagata
     8701 agatttaggt ctagaatatc tagtaactct ttggtaaact ctgaatagga catcttgaaa
     8761 cactcctttg aatggttttc gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg
     8821 tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt caccaacact agatattata
     8881 gatcccttat tttcttcgct tcaaattgtc aaggagtcat cataaatttt gtatggattc
     8941 ttttgtttgg gtagtagtct tctaaataat cttggattaa caccttcatc tctattttac
     9001 ttttatattt gttggagtcg attaattctc atttgattga tcgatgaaac gcttcaatta
     9061 ctgcattgtc aacaggagtc ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca
     9121 acagctcttg ataaataaaa gaccgatgct ggctacctct atcggaatga attaccgtag
     9181 gcaattctcc ttaataaatt tgtaatggac ggacgacaat ctcttttttc atgtgtgaat
     9241 ctaccatata gtaacgtaga atacgagttg ctaagtcaat ataggtggac aaatagaggc
     9301 gaccgtcatt acatggaatg taagtaatat ccatcaccca aacagcatca atttggttct
     9361 gttcaaatta tcgattcaac atttttttac catttctttg attgtttttt ctctaccatg
     9421 agaagatttg cgtcgatgaa atcctcttgt gcataaattc atttcacgca taagaacagc
     9481 tacaacacgt ttattgattt cgattccttc atcgtaaagt ttttgagtga tccctggaga
     9541 tccgtatcga tctttgtgtt cgtagaacac tcgtcttcct cttttagtcg tttattttcc
     9601 tgttcaagaa tgtagaaatc tgctttatca ggtaatcttt ttccttgtcc agcaaaagca
     9661 tttttcccgt atcgacgata ttctgaaacc catcgggaaa ttgttggacg agagatattg
     9721 tattcttcag caattttctt ggtagttact tttttctcaa ggatatcctg tgaaatcttt
     9781 agtttaaatt tacgatcata tgtttttctc aatagagttc attccttccg gtaacgtaac
     9841 tgatagtcaa gaaaagtgta tcatagccca acttagtatc agggtttgtc aactaaactg
     9901 tggaagttga tagataagag tttttaacaa ccaaaattct cttgactatt tttgaaatca
     9961 gacaaatttt gattggacac atagttgaat aaatctaccg tcgttacgga aggtaaatcg
    10021 catgcttttc aattctagag gagaagtcct cgttgaagta ccatagccca gttctggatt
    10081 cgacctcctg cccacttcat acgtaaataa agtaatttta ggagtgagac tgtgacaaaa
    10141 gttattttta agctaaaaac gaattaaaaa acgttgataa ttaaaatcat tcgttttaga
    10201 ttctatgaat cattatagaa cgctgtaaaa tcgatacttt ataattataa agaagatagt
    10261 ttcaaattta ttgttcgaaa tttgaaacta tctttttact tatgtcacag actcccttag
    10321 ttgagttatt ctttaatttc gaatttcgag atagctagat atattgcaag catacattct
    10381 tgataacaat cttgatcata ttttccagtt ctccaggatt gcttatgaat caaagagcta
    10441 aatcttttga gaattgcctc aacagcttct tcatttccag cctttgctaa aatgaaatct
    10501 tcttttaaag aattcataaa tctaccttct tcccatggac ttataatcta tttctttggg
    10561 aaggaggaga acgagcaaag aagcatacaa aattagttat tatgcagata ttaaaccagc
    10621 attttttgaa tttttttgta aagtcgatgt tttaaatttg tcgcaccctg tcttgtaatc
    10681 cccagaacct ttccaatttc tttatcagtt ttatcaaaga taaatttttc gataagaaaa
    10741 atttgttcag gttctttcaa caaaggaagg atttttgcta attttatatc ttcaacaagg
    10801 agagtcacat ttaaaacatt aatcggttgt ttaaatttta tagtatcttt agtaaacaaa
    10861 tatggtggga gttgatcaag ttgttcatgt ttttgaattt cttcttgttt tttataataa
    10921 gtattagcgg tgttcaaaat gaccttttca aagaatcgaa tcaactgggc atttacgcgt
    10981 tcgtcgttat ctgacatatt gcacacctcc tccattaaga tttaaaggga aaagaggcaa
    11041 aaaacacgaa aagaagctaa tgcaattatg ctgcgctgcg aataacttct ataagctcac
    11101 atgcattgat aggactgctg atgtttattt tcaaaaatgt ttttttagtt gataatggat
    11161 agttatcttt atacttagaa aagaaaattc atatatcttc ttaaatatca aacatgtcct
    11221 tatggctaag aaaatgtctt gagcttttta cgtataataa atcttttcta aaacctcctt
    11281 ttggataata ttcttattcg tgtatagatt gtatttttta tttcttcccc cttcataatt
    11341 aacaactaga aaagagttcg aatagacacg tatttgaacg aaaattttta ttggattccc
    11401 aaattggaat atttagaaac gaaaaattcc tattatctgt atagtgctta gataataacg
    11461 gatcataata attattgtct ttgaactaga tatatgtaaa tagtattcat catgatgaaa
    11521 aatgatgagt tctcggtgat aaaatgtact ttttacggtg gaatatacat gaaagcgata
    11581 agtggatatt ttataatgaa gtaagttagc ttattttgac agaggaatag tattttagat
    11641 aacgtataaa atactattta atgatgtaga aaggaaagag agaaacaaaa gtgttttcag
    11701 ataaagaagt cttattaaag tctcttcatg tttgtttaaa gttacctatc gtggttttta
    11761 attccgaatt cagtattata aaagagtatc gctcagatag aactacttat tttttttatg
    11821 attataaaaa gttactaact gataatatta aaaaaactaa agatttccat ttctttac
//
LOCUS       DACCXW010000090        11827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCXW010000090 DACCXW010000000
VERSION     DACCXW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.091"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(113..490)
                     /locus_tag="IVY71_002057"
     CDS             complement(113..490)
                     /locus_tag="IVY71_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986102.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(790..1671)
                     /gene="galU"
                     /locus_tag="IVY71_002058"
     CDS             complement(790..1671)
                     /gene="galU"
                     /locus_tag="IVY71_002058"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7986103.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1714..2733)
                     /locus_tag="IVY71_002059"
     CDS             complement(1714..2733)
                     /locus_tag="IVY71_002059"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7986104.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3366..4370)
                     /locus_tag="IVY71_002060"
     CDS             complement(3366..4370)
                     /locus_tag="IVY71_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7986105.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4383..5432)
                     /locus_tag="IVY71_002061"
     CDS             complement(4383..5432)
                     /locus_tag="IVY71_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7986106.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5449..6270)
                     /locus_tag="IVY71_002062"
     CDS             complement(5449..6270)
                     /locus_tag="IVY71_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7986107.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6257..7036)
                     /locus_tag="IVY71_002063"
     CDS             complement(6257..7036)
                     /locus_tag="IVY71_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7986108.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7053..7523)
                     /locus_tag="IVY71_002064"
     CDS             complement(7053..7523)
                     /locus_tag="IVY71_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7986109.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7535..7954)
                     /locus_tag="IVY71_002065"
     CDS             complement(7535..7954)
                     /locus_tag="IVY71_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7986110.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8035..10803)
                     /locus_tag="IVY71_002066"
     CDS             complement(8035..10803)
                     /locus_tag="IVY71_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7986111.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            11003..11227
                     /locus_tag="IVY71_002067"
     CDS             11003..11227
                     /locus_tag="IVY71_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986112.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3434 a         2481 c         1917 g         3995 t
ORIGIN      
        1 aaaaagattc ttttatataa gtgaaaaaag cttttttcca ccaattaaat aaatctctca
       61 tctcaatagt ttatccatta ttttatcttt ttttgcgtat acctactaaa tattaccgtc
      121 ttcttctcgt tcttctactt tgttcgagag aatctgtact agaaaaagta gttgtatgtc
      181 taccttttct gcctatccga gactttcttt tcatatatgg gatataaatg attagataca
      241 gtacagcaaa aaataaagca aatacgcaag cttcccaact acctaacgcc aattcaacca
      301 tcaaggtagc aaccccaacg gatgctgcat accagataaa aatcagaaac gtttcttttt
      361 ttctgaccat gcgaaacatg ataaatagac taataagcaa tagtggcaac acaaatatcc
      421 aattgcttcc ttccatcagc cacttgagta tatcaagcag cgactgtgca acagcgacta
      481 attttcccat agtgaacaca cacctttttt tataattaaa aaattcgcct ttttattcta
      541 ctatgaatct tctggcttgg ctttcatttc tcttatgtaa tcattttctt ttatacaact
      601 taaaaaaaaa ttaaaccatt agcataatat tttctgaatc tattcacgaa agtctttttc
      661 tatctttcgt tttccacgac tcaactgatc tagagtcaaa aaactaaaac gacagttgcg
      721 caaatgacgc acaagctgcc gctttaataa caaatagcct ttcattctca gcaggccact
      781 tatttccagt taactttctg ttcttagctt tttactcaac tggataatat atgcacttaa
      841 tgaagtccca atttccgaat gttgaagtcc atattcaata tttgtcttca tatatcctaa
      901 cttatcgcct acgtcgtatc tttttccttt aaactctctc gcatagaccg cctctgtctg
      961 gttaagagtc tgaagtgcat ccgttagctg tatctcgttt cctgctcctg gttcttgagt
     1021 ttctaatatc tcaaatattt taggagtcag tagataacgt ccgataatgg ctaaattact
     1081 tggtgctttt cccatttcag gtttttctac aaaccctttt actctgcaac gacccttttc
     1141 gctatctgtt ttaggatcaa taattccgta tttagatgtt tcattttctg gtacacgcat
     1201 gacggcaaca gcggaagctt gtgtttcttc ataatccatc atcaattgtt gagtcaatgg
     1261 gatatcatct ttcatcaaat catcccccag catgacgaca aacggttcat ttcctataaa
     1321 tgcttttgct tgaagcacgg catcccccag tcctcttgga tgagattgac ggatgaagtg
     1381 aagattgact tgagtcgttt cttcaactaa agcgagtaat tctgtttttt ccttttcccg
     1441 caaattgttt tccaattcga tatttgcatc aaagtgatct tcaatgggtc ttttttcttt
     1501 tcccgttacg atcaagatgt cttcgattcc cgaatccaat gcttcttcca caataaactg
     1561 gatcgttggt ttatccacga ttggaagcat ttcttttgcc atggcttttg ttgccggcaa
     1621 aaaacgtgtt cctagcccag cggcaggaat gactgctttt ttgaccttca cttgctgttc
     1681 ctcctaaacg acgattaact attgatcatc tatttaacga agagaaaatt cattctccat
     1741 ttttccgtca cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa
     1801 aacattatag attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac
     1861 tgctgctttt gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc
     1921 cagcttatgt ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt
     1981 acaagtaacg atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc
     2041 catcgcgaca ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc
     2101 atcatctccg aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag
     2161 tgctgcacca gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt
     2221 catgaacagt ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt
     2281 tgtgatatca tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc
     2341 ctgtcttttt tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc
     2401 ttgttctagt cctttacttg catgtataat gattggctta gtattcattt taccaataag
     2461 ttcctgtgcg actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt
     2521 atccactgct tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg
     2581 taaaaaatgc tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc
     2641 ccaaatacgg acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga
     2701 tccaggacca agaactgcga ttttttgttt catggatatt cctccaaata aataattgat
     2761 aatttccatt atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt
     2821 ttgtttactt cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac
     2881 attttatttt tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg
     2941 ttactaccag cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa
     3001 atacataaga gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt
     3061 ctctgttgac ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct
     3121 atgtaaactg gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa
     3181 atagtactat tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata
     3241 agttgaactg tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta
     3301 aaaaaataaa gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt
     3361 ctatcctaat aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac
     3421 tgctccacat gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca
     3481 cgtccgcgat atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta
     3541 taattgctgc aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca
     3601 tcttcgccta aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga
     3661 agaaactcgg cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac
     3721 atggcatatg aataaacagc tccccattga ttccctgctc caataaaata atgcatacta
     3781 tcacgtcgat gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc
     3841 actaaccctg ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat
     3901 tctccattca aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca
     3961 ggagctggat aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg
     4021 ataaatccaa tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct
     4081 tgagttgtac cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt
     4141 ccagtagtgt aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc
     4201 atataagtga cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca
     4261 gcgtcaaatt tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct
     4321 agtgcgcctt ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc
     4381 tcttataatt tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca
     4441 atacctttag ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat
     4501 acagacagat attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt
     4561 tttgtggtaa tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc
     4621 gaacgttcct tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc
     4681 tccaggtatg ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt
     4741 gtttcagtca atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg
     4801 gtgacgaaac gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt
     4861 ttttgaataa tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt
     4921 tctatttttt tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac
     4981 tgtaaaacag tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct
     5041 aaatcaataa tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca
     5101 acaacaggta acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc
     5161 ccgctttgtg acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa
     5221 aaataaaaag gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc
     5281 gctgctaaac aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt
     5341 ttcatatgtt ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt
     5401 tcttcataga tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc
     5461 tatccacgaa ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac
     5521 ttctttcttc aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac
     5581 gatatcatca aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg
     5641 aatattgatg gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga
     5701 cgcgcctaat gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt
     5761 tcctaagcga tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa
     5821 aatcaaaatg aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac
     5881 acctgtggca atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg
     5941 tcccatcaaa gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt
     6001 attttcttcc tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt
     6061 gttaaaaaac tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa
     6121 ctttttcaaa atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt
     6181 ccaagaaaac tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat
     6241 tttgctgttt ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa
     6301 gtggtagttg acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa
     6361 tagctactcc ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat
     6421 atggtgcaac ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa
     6481 tcaaccctgt agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat
     6541 ttccgatcgt attgcttccc accatataag aaaccattac cacaacacca agcatcaagg
     6601 aaagaccgat accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg
     6661 cataattgtc tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca
     6721 atatcaatgt caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc
     6781 cagctgtaat agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg
     6841 aagcacctac tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc
     6901 cagtttttac atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa
     6961 gcaacgatgt ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga
     7021 ctgcttgtac aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt
     7081 ttatctgctg gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag
     7141 cgttcctcct cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt
     7201 gccttgatcc ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat
     7261 gcatcctcga tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc
     7321 aacgcggcaa tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct
     7381 aatttgatcg ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata
     7441 cagtctgcgc ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg
     7501 tcgactcttg tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct
     7561 gattgagccg ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga
     7621 tcaaaacagt tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca
     7681 aattcaatcc ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt
     7741 tgacacttcc gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa
     7801 aaagctgcct tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag
     7861 taatagaatg atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt
     7921 ttccatggga agctaagata atttctctat ccactttttc atctcctctt ttcacttaca
     7981 catactaaaa aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa
     8041 ttcttcttct atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat
     8101 ctcagaattg gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat
     8161 agcagataac ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat
     8221 tggcatttgt cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt
     8281 tttgggattg ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat
     8341 ttctcccatc accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga
     8401 aaaattacga atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc
     8461 caaccattca gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata
     8521 gtcgatattg gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga
     8581 aaataatggg tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt
     8641 caactgacaa gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc
     8701 gattccagtg aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag
     8761 tcgccattct ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt
     8821 tttttgcagg cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa
     8881 aagcggtacg gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc
     8941 tactaatatg atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga
     9001 agcaatctgc tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg
     9061 taataaacga tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat
     9121 catcgctttt ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag
     9181 taaaatccga tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat
     9241 tcgttggaca tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt
     9301 gattagttgg ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag
     9361 atagtaactc aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat
     9421 ttgtcgaaag gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg
     9481 tctactggtc tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc
     9541 ttcgcaagaa tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact
     9601 tttaacgatt cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc
     9661 ttgcgtaata tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc
     9721 tttttgcaaa tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc
     9781 ttttgctgct gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg
     9841 acttgttaaa agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg
     9901 ttcttcgaga aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag
     9961 tgcaggtagt tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt
    10021 aatctcttca gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc
    10081 tcccatacga taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg
    10141 tgttaagcga tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc
    10201 ttttttattt tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt
    10261 caaaagttca ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc
    10321 ttctagcaga tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc
    10381 tgtgccactt tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt
    10441 tttgatttgt tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa
    10501 gtcttttggt tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa
    10561 attcgtgcgt aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg
    10621 tcgagaattg attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac
    10681 tgtgtttctt tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat
    10741 atgttctaaa tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa
    10801 caacttattc acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta
    10861 ataaagaatt gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt
    10921 tattctaaaa aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga
    10981 ataaggaggc tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc
    11041 ctggaaaact tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt
    11101 gctttatatg gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc
    11161 cgaaacagga tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac
    11221 aaaataagat tcacagattt caactttcct gtctgttttt agctattaca aatccttata
    11281 tgtggtataa caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag
    11341 agtaaagagg tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt
    11401 ttcccattga ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt
    11461 tattttcaga tcaaaatctc aagtggcttt tgacttgatg tcgggaggtt gtcttcgttc
    11521 catcaaacct gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct
    11581 aagatagtac ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa
    11641 aatcaattag atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc
    11701 aagaacaaat taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt
    11761 ttttatgcag aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga
    11821 gactggc
//
LOCUS       DACCXW010000091        11719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCXW010000091 DACCXW010000000
VERSION     DACCXW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.092"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            5..2377
                     /locus_tag="IVY71_002068"
     CDS             5..2377
                     /locus_tag="IVY71_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986113.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="IVY71_002069"
     CDS             2438..3898
                     /locus_tag="IVY71_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP7986114.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="IVY71_002070"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="IVY71_002070"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7986115.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6353..6946
                     /locus_tag="IVY71_002071"
     CDS             6353..6946
                     /locus_tag="IVY71_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP7986116.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="IVY71_002072"
     CDS             6959..7858
                     /locus_tag="IVY71_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7986117.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="IVY71_002073"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="IVY71_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7986118.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="IVY71_002074"
     CDS             8724..8984
                     /locus_tag="IVY71_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986119.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="IVY71_002075"
     CDS             9429..9635
                     /locus_tag="IVY71_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7986120.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="IVY71_002076"
     CDS             9683..9886
                     /locus_tag="IVY71_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986121.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="IVY71_002077"
     CDS             9902..10339
                     /locus_tag="IVY71_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986122.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="IVY71_002078"
     CDS             10332..11039
                     /locus_tag="IVY71_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986123.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="IVY71_002079"
     CDS             11419..11598
                     /locus_tag="IVY71_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986124.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         4393 a         1952 c         2450 g         2924 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcc
//
LOCUS       DACCXW010000092        11714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCXW010000092 DACCXW010000000
VERSION     DACCXW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.093"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            507..1070
                     /locus_tag="IVY71_002080"
     CDS             507..1070
                     /locus_tag="IVY71_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986125.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1131..1496)
                     /locus_tag="IVY71_002081"
     CDS             complement(1131..1496)
                     /locus_tag="IVY71_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986126.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1572..1817)
                     /locus_tag="IVY71_002082"
     CDS             complement(1572..1817)
                     /locus_tag="IVY71_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986127.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1887..2141)
                     /locus_tag="IVY71_002083"
     CDS             complement(1887..2141)
                     /locus_tag="IVY71_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986128.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2305..2628)
                     /locus_tag="IVY71_002084"
     CDS             complement(2305..2628)
                     /locus_tag="IVY71_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986129.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2815..2888
                     /locus_tag="IVY71_002085"
     tRNA            2815..2888
                     /locus_tag="IVY71_002085"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2849..2851,aa:Ile,seq:cat)
     gene            complement(3273..3770)
                     /locus_tag="IVY71_002086"
     CDS             complement(3273..3770)
                     /locus_tag="IVY71_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7986130.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3810..4619)
                     /locus_tag="IVY71_002087"
     CDS             complement(3810..4619)
                     /locus_tag="IVY71_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7986131.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4953..5810
                     /locus_tag="IVY71_002088"
     CDS             4953..5810
                     /locus_tag="IVY71_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7986132.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5803..6705
                     /locus_tag="IVY71_002089"
     CDS             5803..6705
                     /locus_tag="IVY71_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7986133.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6738..7088
                     /locus_tag="IVY71_002090"
     CDS             6738..7088
                     /locus_tag="IVY71_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7986134.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7122..8060
                     /locus_tag="IVY71_002091"
     CDS             7122..8060
                     /locus_tag="IVY71_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7986135.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8138..9031
                     /locus_tag="IVY71_002092"
     CDS             8138..9031
                     /locus_tag="IVY71_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7986136.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9069..9632
                     /gene="amaP"
                     /locus_tag="IVY71_002093"
     CDS             9069..9632
                     /gene="amaP"
                     /locus_tag="IVY71_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7986137.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9646..9834
                     /locus_tag="IVY71_002094"
     CDS             9646..9834
                     /locus_tag="IVY71_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7986138.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9847..10749
                     /locus_tag="IVY71_002095"
     CDS             9847..10749
                     /locus_tag="IVY71_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7986139.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10781..11023
                     /locus_tag="IVY71_002096"
     CDS             10781..11023
                     /locus_tag="IVY71_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7986140.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11279..11650)
                     /locus_tag="IVY71_002097"
     CDS             complement(11279..11650)
                     /locus_tag="IVY71_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986141.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4149 a         1980 c         2227 g         3358 t
ORIGIN      
        1 cattggtcgt tgaatgggct gaatagtagt gtataaaaca attttgtgga gcgtgaagct
       61 tttatggtag aggaaaatac gaataaagtg atgttgataa aaagattcct atcttgaata
      121 tgaatcgaga tgtcttgaaa atgaatgagt ttgaccaatc attatcctat cgaaatttac
      181 agtttcgttc gattggtctg tcaattaatg aaaaagtaaa aaagaatatg ttctaggaag
      241 agggcggcac atctaaattt ttaagtaacg gacagttcaa atatcaagca ttgaaagagg
      301 ctatcctact gtttgaagga atacgaatat ataaaaacga caaagaggaa aaagtagttc
      361 attaaatccc ttcaagtgat gaaagaatga gatctaataa aacctgatct ttcatctgtt
      421 tttttaataa cgtaataatt tttttatttt ttgtgctttc gccgttattg tagagtgatt
      481 tagcattctt atttttaaaa ataactgtgt tatatattaa aaaaggcata atagttataa
      541 gcttttttaa agaatattta gaaattatta attttcgtct gaaaacaaaa aaagaaagcg
      601 ttattatttt agtctatttt caggggggga accttatgat aaaaaaacta tttttactat
      661 caatattaac gctagcaatg gcaggttgtt ctgactctgg cttagcaact gaactgatca
      721 caaaatcaac tgaagccacg acagaggagt taaaatgtgg aaacgatcac agtgactaca
      781 gaggcttaaa tcgacgatgc atgggaatat taggcagaag caattgtaag catcgatgta
      841 gtaatcgaag aaggtgtaga agaagcaaca gaaaaatcaa aagaaagatc aaaagaagca
      901 atctgtgctg tgcatcgtat acagacgtcg aagatgcggt acttttaaac cacgtcagga
      961 gatgttcatt tatgtctagg gaagttggcg atgaaccaaa actagagtac gcttttagtt
     1021 ttgcacgtga ggatgaagat aaccctaaaa cttacgacat tttagcttga tctaagatgg
     1081 ttaaagcttt ttaatgaagt aagaggagat agaaggcttt tctagttata ttaggtaaga
     1141 taaattcctt tttgctccaa atttgctaaa cattctgcta gataaacttc atcgtgttcc
     1201 ggataaatcg gcacgttgcg ctctacagta ggtaaatcaa aaaatgcttc gcatgatttg
     1261 ccacgataca taggattttt ccactctggt gctgggctgt aaccacgttt ttccatttca
     1321 tccatgacaa gctgatgata ttggaataat ctataaggag aatagtgaaa aacataatcc
     1381 acagtagcat gtttctttcc ccagccattt cctcttaatg cacagcattc ccgatgttgt
     1441 cctaaaagct gttgacgagg aagctttgaa atcaattttt catgccataa tcgcatagaa
     1501 atgtctcctt tatatataga ttgttaacct tagacgttaa atgaaaaaac cagcaccaag
     1561 gctggaaaaa atcacttcag tttgcttgct gtttctgagc gaccaattag gtaatcaacg
     1621 gaaatatcgt aaaagtcagc gatatagatt aattcaatga tgcttggaat ttcttctcct
     1681 tcttcgtact tacgatattt gaataaagga atacttaacg cgctagaaac agctacttga
     1741 gataaagatc ggccagttct taagaaattc agacgacaac gtaaagtatc cagtaaaata
     1801 gcttcttcga tactcattta atcacctttc attatagtag aatagttata atgactatag
     1861 aaagctacac atgcaatcaa tagtactcaa tgttttttat ttaaacgtat caaataaaga
     1921 atgacgatca ataaagacaa aaccagtcct gataattctt ctataaaaac actatcgggt
     1981 tcaaagtagt tgtaattaaa aactttccac ataaagtaga tagccggaag agtcaaaata
     2041 gctaaataaa accgttcttt gttccaattc ataagataaa caatacttgc gaccagtgcg
     2101 accgcaggca cccaaaacaa aactctcacc aacgtaatca tgtaacggac tcctcaacga
     2161 ttcatataat aaaagataac atgttattgc atacatcaca aatgaaatgc taggaaaata
     2221 aaagaagaat agatcaatga aaaatgaaaa agcagaacag ttttctgctg ataagcagga
     2281 actgttccgc tgaatgtgtc ttatctatcg agaaaaaatc aatggattga tatctaagta
     2341 attcaataca tataaaaaga ggacgatcgc attcactaaa aaggaaagaa tgatgatccc
     2401 atagcgaatg gaaacatatt taacaaatac ttttgtttga atacgggcat atctttttcc
     2461 tttatagctg agcatcatga caagaagcaa aagggtaatc acaccttgaa aaatcattcc
     2521 aataaacaga attcctgagg tttgataatt taattggaga aaataagctg ctgccgcaac
     2581 taaatcgaat aaaatcgcaa taaatggcaa aataatcact cctaacattg agaataaata
     2641 aaagaataac attttttcta aaataagcaa ataaaaatta aagtaacaaa atagttatga
     2701 cacaagtaaa agaaataaga ataacaatat tattttactt atattttgaa acttgtgaga
     2761 gtttgtcatt cgtgctatag ttgaaaagta acaaaaataa attttattta tgatgtacct
     2821 ttagctcagt tggttagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac
     2881 aaggtacata agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta
     2941 aaaacagctt cttatgtttt tacttggctg ttcttattac tgcagatgca agcttttgaa
     3001 taccgtttaa ggaagaggtt gtgaaatcag cgttttgacc tgagtattag cttaacaaac
     3061 ggaaaaatag ttcctcatat tttgccgttt gttaagctaa taccgagggt cagctgattg
     3121 aacaccattt aagaaagagg tcgtgagcct gccgtttcac tctgtgtcat aaaaaagcca
     3181 atctactttg atagaaaaag cacgtaggac taaaacagtt ctgcgtgctt tttttattgt
     3241 gtgttaaata tcgtaaatga agatagatat ttttatgatt cgacgataga gacgttgaaa
     3301 tctttcaaca gctctaaagc ggattctccg tgttttctac tggagactaa cattggatca
     3361 acaaaaaagt ctgcttcagg aaaagcactc tgcaggccta tcagattaga gagtacacac
     3421 aagtttgttt caatcccgac tacttcgatc tctttttgtt cttctttgct ttcaaataaa
     3481 gtttcctgga tttcagctaa agtttcaggt ggaatcgtaa agtaatactt tttaatcact
     3541 cgttcatttt cttgtggaga aagaagaggg atcaattgca aatcttctcg ttcttcttct
     3601 ttgtccttca gttcaactgg tatatcccgt gtgaagagaa tatattcatt attttggcgt
     3661 gctttagcta atctttgagc aatacgttct gccaattgat ctgactctgg aatataaaat
     3721 tcgctgtttg gatcaagaat atgattttgc atatcaataa taactaacat tttctttcct
     3781 cctacattca tatcgtcatt ttattctttt taatagaagt tttttaaaat ataagcaaca
     3841 ccagcttctt cattcgtcag tgttacttgg tttgcaattt cttttacttg tcgctgtgca
     3901 ttccccatcg caatactcaa cccgctgtaa ttcaacatag caatgtcgtt ttcttgatcg
     3961 ccaaaagcca tgatgtcact cgattcgatt ccaaggtaat ccgccagaaa aacaagtgaa
     4021 tgagctttag tgatacctgt aggcgtgatt tctaagaaat gcggatcaga gaatactgat
     4081 tggataccat ctgcttgcag atctctttgt agcttctgta gtttttcttc actcttatct
     4141 gttaatgcta atttaagata gctaagagaa ggatcttcca acatatttaa aatttgatca
     4201 aaagaatggt tctgtaaatt gaaatgttca taataagggt catctttgac tagatttgat
     4261 tcatcgaatg tcagctgatc atcttgggtt tctcggctag caatgatgga tattttcgct
     4321 tcttgagcat agaagaaggc ttttctcagt gtatcgctat ctatttttga acgtctgaga
     4381 actttcgcca tttttccatc cttcatttca gcaatcagtg aaccattgga agaaatcaaa
     4441 tagccaccga ttcctaattc ggaaaaaata tctttagcag aaatgctgtt ccttccggta
     4501 gcgacggtta cgattccgcc attttcatca aatttccgaa gtgcttggat attttcttct
     4561 ggtatctttc catttcgatc aagcaaggta ccgtctaaat caatcgctgc tagtttcatt
     4621 ttatcttcct actttctttt tacatatcta ttttcctgtt accttaagat taaatgagaa
     4681 tttaaaaaaa tgaaactaac ttgctttgtc attatgaatt ttttcaaaaa acattcttaa
     4741 gaaaaaggtg gctatatata gtagtttttg gtcaatacct ttgaataaaa agccatgaaa
     4801 aacgaatagt tagcgcttac cgatgcgttt tgtgataaaa aaattttaaa atattcaatg
     4861 aaaacaaagc atatattttg ctttaaaacc aaatgaggaa tattctagat gtagaacagg
     4921 ttagaaaaag attagaaaaa ggagtgattt tgatggagaa gagatacggc gtagttcctg
     4981 tggtaactgc ttcagacgaa aattatgctc cttacttgag cgtgatgatc gcaactgcat
     5041 tagaaaattg caataaaaca agacgcataa aattttatgt gattgacgat ggtctatcag
     5101 aatatagtaa agaaggattg gaagaaacgg taaataaata ttcaagtaat gcaagtatcc
     5161 aattcctgac agtggaaaaa gatatctacg aagacttcct agtcagtgac catatcacaa
     5221 ctaccgctta cttaagaatt tctttaccaa acttattagc taaagaggat tataagaaag
     5281 tcctttactt agattcagat gtgttagtac tagacgatat cgtaaaacta tatgatgagc
     5341 cgttaaatgg aaaaacaatc ggcgctatta tcgatccagg ccaagtgaaa gctttggaaa
     5401 gattaggaat cgattccgat gacttgtact tcaattcagg agtgatggtg attgacattg
     5461 atcaatggaa taaaaaagaa attacagaaa aaactatcca ttacctcagt gaaaatggcg
     5521 atcgaatcat ctatcacgac caagatgctt tgaatgctgt actttatgaa gattgggaac
     5581 aacttcatcc aaaatggaac atgcagactt cgctgatttt tgaacgtcat ccagcaccaa
     5641 atgaaaaata tgaacgtcag tataaagaag gaaatgaaaa accttcgatc gttcacttta
     5701 caggacatga taaaccttgg aatacgttga aagatcatcc gtataccaat ttatacttaa
     5761 aaaaattagc acacagtact ctaacgaaag taggcgagat caatgaataa aaaagaaata
     5821 gcagtagtcg caagttgtaa cacaaaattc gtaccacatt tagctgcatt atttgtctca
     5881 gtattagata attgcaaccc gtctaaattt gttcggttct atgtgattga cgatgatatc
     5941 gattttgaaa gtaaacagct tttacgcttc tcagtgaaaa acgcacgaat gaactcagat
     6001 gtggaattct tgaaaatcaa taaagaattc tttacaaatg tggtaatcag cgatcgtatt
     6061 ccagaaacag cttattatcg tattgcgatt ccggaattgt tccgcggcac agaagttgaa
     6121 cgtatcttat atatggattg cgacatgatc gctttacaag atatctctaa attatggcgt
     6181 cttgacttcg gtgattcaat cgtagcagct gttgaagacg ctgggttcca tcaacgactg
     6241 gaaaaaatga aaatttcagc taaatcaatg cgttatttca actcaggttt gatgctgatc
     6301 aatgtgaaaa aatggttaga tgaaaatatt acgcaaaaag tattagattt catcgaacat
     6361 aatccagaaa aattacgatt ccatgaccaa gatgcattaa atgctatctt acatgaccgt
     6421 tggttaccac ttcatccgcg ttggaatgca caaggctata tcatggcaaa agcaaaaaaa
     6481 catccaacag cagcaggtga acgtgaatac gaagaaactc gcaacaatcc ttacattatc
     6541 catttttcag gacatgtgaa accgtggagc aaggattttg aagggccaac gaagaaatat
     6601 tacgaaaaat atgcagggat gacggctttc cgttgtgtag ctaaattccc taaatatcca
     6661 aaatatgcaa aagttcaacc aaaaacagat gtagttgtta aataatagaa gaccacaact
     6721 gaagaggggg agtaggaatg gaaattacaa tgtctaaaaa tgcagtagag acgttaattg
     6781 aaaaggttgg ggaaaataca aaaatcgcac tcgcgcttat caatgattcc gatccatttc
     6841 taagggacaa aggtgctttt gcaaaaggca gcttttttca aatcattcct tttgtttcgg
     6901 aatttggaga atatgcgact aaaatcgagc atccattatt ggacatctat acatctaaat
     6961 tagagcaaaa ttatttcggt aaacgattga acatggactt caacaaacag cttgacagct
     7021 tttcattaga aaatgaaatc gcagtactcg attataatat caaattaaag aactgcttct
     7081 tcagctaata gtttcgagaa agtccaaaaa ggaggtatac attgagatac tactttgtgg
     7141 atgaagaaaa gaatgtaata gaaacagatg aaaaaaaata taattggctc gtacttgata
     7201 gtgcaaagcc cgaagagatt gagcaagcaa tcgaagaatt cgagcttcct gatgatattt
     7261 tcgtaggaac cgaatatccg gaagaagttt ctcggatgga gcgcctttat gagacgaaac
     7321 tagagcatcc attttctttg gtagtgatta atctagatca gtcagataat cgtatcgaaa
     7381 aaaaattaac gccaatatct tttgtacttt ccgataatct cttgattact tgtatcgata
     7441 gaaaaaccga atttgtggat cggctgctga caaagcatgg aaatcattta aactcgtttg
     7501 aaaagatcat cacgtataca ttattagata tttacaccca ttacgtgaaa gaactaagag
     7561 agatgaaaaa gcgaattgac gctttggata aagcagctcg taaaacaacg gaaaatgaag
     7621 aactgtataa acaggcagat cttgaaagag acatcgtata tatcgatcat accttaagag
     7681 atcaacggga aacaatggat caattatgga attctgaaac tttcatcaaa cgtttagacg
     7741 atgaacagct actctatgat gtccatttga gacagcgaca aacagaaaaa atgatcgaga
     7801 tctatcgaga tttattagag agtattggtg atctattcaa cgggatgatg gacaataatc
     7861 taaatcatat catgaaatat ctagattcag ctgcattaat catttccgtt ccggcgttgt
     7921 tcgcagggat ttggggaatg aatacaggag gattaccggg taaaagttct gctctaggct
     7981 ttatactcgt agtctttggc tctttgatag cagcagtagt tttaggttac catttatacc
     8041 ggaaagatta tacaaattaa agaatctatt taaaacaggt atcaaacaaa gaaatttgtt
     8101 ttggcaatac aatttaaaaa ctaggaggaa tacagcaatg acagaaccaa aattaatcga
     8161 cccgcgaaac ttgtaccata cggagaaatt tcctaaacaa gatcaggata caccagcttt
     8221 acagcagaat atgattcctg ttcctgattg tggagaagaa tcctatgaag gcaataatca
     8281 actagaaaac cgccgtgtac taatcactgg tggagattca gggatcggtc gtgcagcagc
     8341 gattgcattt gctagagaag gggcagatat cgctcttcaa ttcttcccag gagaagaaaa
     8401 agatgcagag gaagtagcaa aatacgtgaa agatgcagga cgtaagattg tcttactacc
     8461 ttttgatctt cgtgacgaac aagcacctaa agaaatcata gataaagccg tgagtgaact
     8521 tggcgggcta gatacgatgg tactaaacgc aggacagcaa atttcttgtc agtctattgc
     8581 tgagttacca cttgagcaag tcaaagacac atttatgata aacatcatcg ctatgtttgc
     8641 tttagtgaaa gaagctgttc cgcatatccc agcaggagga gcaatcgtaa caacaacttc
     8701 tgttcaagca ttcaatccaa gcgaacattt attagattac gcagcaacaa aagcttcaat
     8761 cgctaacttt acgatcggtt tagcaaaaca gcttgcacct aaaggaatcc gtgtaaatgg
     8821 tgtagcacct ggtccaatct ggacaccatt acaactagac gaaggacaac cttctgattc
     8881 gatccctgaa tttggtcagc aaagtttact agaacgtgca ggtcagccag gagaattagc
     8941 accagtttat gttttcttag catcgaataa agcaagttat gttacagccc aaatttacgg
     9001 agtaactggt ggagaagcga tcaacttata agaagttgat cgtttcaccc ctatgaggag
     9061 gtgcgaaaat gaataaaggt gtaaaaacga tcggagtgat cggaacatta cttttgctat
     9121 ctgtgctttt gtttgcgata ctattaagtg caacctatcc attgcctttt cagctagaga
     9181 agtttcgatt cttttccatc actaattcat acgtgcaaca atatgtattt tggcttgccg
     9241 ttgcatttgc tattttgaca attatcacgt tacttgtttt cctgttttat ccaaaaacaa
     9301 tcggaaactt tctactgaaa catggtgatg gggagttgac gttggataaa aaagcaattg
     9361 aaggaatggt tcgttctcac ttgcatgaag atgaattcat ccattcacca aaagtaaatg
     9421 tcaaagcaac gaaaaacaag atcgccgtca atgtaaaagg ggagttgaaa cgaacttcag
     9481 cactcattgg taaaacagga acattgatga aagaaatcga aaatgaagta caacacatat
     9541 tgggaacaaa agagccagtg aaagtatcgg tgaaatacac aggctttgaa gaaccagcta
     9601 aacgaagcaa tgaccgcaaa cgagtagaat agggggggac caaaaatgaa agaactattt
     9661 tcgacataca agctccctat catctttggg gtacttggat tgatattagc cgttttatgg
     9721 atcaccgtgg gctttttcaa gaccttattg atcttgatca tggcagcgat cggcgtcgct
     9781 atcggttttt atctgaaaca aacgaaaatc ttagatcact attttaaatc ttaggaggaa
     9841 acatccatga ataacaataa caaaaataat aacaataaaa tgaacaacca aaaaccagtg
     9901 tctgcacatc cagacccaac gacaccaaac caatcaaatg gtgtgaacga tgaaaagaac
     9961 aaaacagcac cagtatctgc acatccagat ccagtagtcc ctaacgttca acaaggagta
    10021 gaagagaaga aacatgatgc agaaaagcat gcacctcatc atccaggaac tccagaatct
    10081 aaagttccag aacaacatga tgcaatcgat ccttctaaac ctgtggaaga gcaaaaacat
    10141 ccttctgacc atgcgaaagc acaagaaaaa cttagttctg accacacaaa cggacaagga
    10201 aaacttggtt ctgatcatac aaataaccaa ggaaagcatg attctgaaca ggaagattca
    10261 atgcgcggtg agctgaatta tgaagataaa gttgtgcaaa aaatcatcgg aatcgcaatt
    10321 gaacaagtag acggtttatt atctgcaaac ggtggtttct tctctaatgt ggcaggaaaa
    10381 cttgtgaata cagataatgt aacggctggg atcgaaacag aagtcggtaa aaaacaagta
    10441 gcagttgata tggatgtcat tgtggaatat ggcaaagata tcgaaaaaat ctttgaagaa
    10501 atgcaagaag taatcggcaa agaagtaaaa aatatgacac atttagaagt gatcgaagtc
    10561 aacgctaacg tagtggacat caaaacaaaa gaagaatttg aaaaagatca agaaacggtg
    10621 caagacaaag taactgacgc agcgaaaaaa accggtgaat ttgcttcgaa tcaaacagat
    10681 aaagtaaaat cagcagctgg aagcggcgca caaaaagtaa aagaaaatac agaaccacgg
    10741 gttcagtaat aaggaagtaa aacattagga ggaacgaaac atgggattta tttggtcact
    10801 tatcgtcgga ggaattatcg gagcaattgc aggagcaatc acgaataaag gttcttcgat
    10861 ggggattatt gcaaatgtcg tagcaggttt agttggatca gcacttggtc aagcattatt
    10921 aggttcttgg ggaccaagta ttgcaggaat ggcaatcgtg ccatcattga ttggtgccat
    10981 cattttagta ctaatcgtat cattctttgc tcgaaaactg taaaaagact tttaaaaaga
    11041 ggtcgtgaaa aagctgttct tcatcaagta ataagaacag ccaaacaaaa atagcttctc
    11101 atatttttca tttggttggg catattatcg aagatgcaag cttttgaaca ctgtttatac
    11161 ggaataagag gctgtgacaa gacttttgtc acagcctctt atttatgcgg gcaaattccc
    11221 aattagattt ttaaaagttt ttccttaaat gtgtctttat tgatacagag aagcttcttt
    11281 aataaaacaa tgcctcccta aattttcttg agataggaaa ggtgaacata caaaataata
    11341 cgaccacttt gaaaacccag atgatccaca aatggatcgt atgatactga tgaagtaaaa
    11401 agcaatggat cagaaactca aagagattga aaaagacaag gaagaaataa tatctctttt
    11461 ttactcttgt ctgttttagc caataatagc tcgtaaagta atctagacaa acaaatagaa
    11521 gtaaacaaag tttcgtaaga gcagcaatcg tatcatgttg ctctgctaga cgattgacga
    11581 gcaggaaaaa tgttggtaat ggaatataga tcacgagtaa tgaaagaaaa aagtaaacaa
    11641 taaatggcat actattcctc ctaattgtgt gagttcgccc tcattttaaa ataacgatat
    11701 ttgtttgtga agct
//
LOCUS       DACCXW010000093        11401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCXW010000093 DACCXW010000000
VERSION     DACCXW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.094"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            205..411
                     /locus_tag="IVY71_002098"
     CDS             205..411
                     /locus_tag="IVY71_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7986142.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(613..1614)
                     /locus_tag="IVY71_002099"
     CDS             complement(613..1614)
                     /locus_tag="IVY71_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7986143.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1619..3532)
                     /locus_tag="IVY71_002100"
     CDS             complement(1619..3532)
                     /locus_tag="IVY71_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7986144.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3700..4206)
                     /locus_tag="IVY71_002101"
     CDS             complement(3700..4206)
                     /locus_tag="IVY71_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7986145.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4366..4806
                     /locus_tag="IVY71_002102"
     CDS             4366..4806
                     /locus_tag="IVY71_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7986146.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4832..5989)
                     /locus_tag="IVY71_002103"
     CDS             complement(4832..5989)
                     /locus_tag="IVY71_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986147.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5992..6348)
                     /locus_tag="IVY71_002104"
     CDS             complement(5992..6348)
                     /locus_tag="IVY71_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986148.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6646..7620)
                     /gene="sgrA"
                     /locus_tag="IVY71_002105"
     CDS             complement(6646..7620)
                     /gene="sgrA"
                     /locus_tag="IVY71_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7986149.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7816..8655)
                     /locus_tag="IVY71_002106"
     CDS             complement(7816..8655)
                     /locus_tag="IVY71_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7986150.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8840..9727
                     /locus_tag="IVY71_002107"
     CDS             8840..9727
                     /locus_tag="IVY71_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7986151.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10321..11015)
                     /locus_tag="IVY71_002108"
                     /pseudo
     CDS             complement(10321..11015)
                     /locus_tag="IVY71_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(10999..11397)
                     /locus_tag="IVY71_002109"
     CDS             complement(10999..11397)
                     /locus_tag="IVY71_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7986152.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3399 a         2285 c         1705 g         4012 t
ORIGIN      
        1 tattcccata ttttctatta ttggtctaga cgtatgatgg taaactttgt acctatctga
       61 ttgctgaatc attaattgct tatcattagc catctttgtt tcaagttcgc tagatgatca
      121 ttttattcac atcctaacaa acttgtcaca tttatagatt cttaatctat ttatcataca
      181 attataaaaa aggaggtgct gcttatgaaa gctatccata tttctttgct ttttttcgct
      241 agtgcattga ttttatttgc tattcatctt tgttcccagc cttatatcaa taacaacgga
      301 atattagtgg aaccagcatt cttttgctta ccactcggct ttttgtccct tcttgcctcc
      361 gcaggcttcg gatttgttgc tttttttaaa cgatcaaaac aacaaatctg acttataaaa
      421 tttacgtaaa agagatcgtg acaataatcg tgtgatattc ccaaatttcg aataaaaggt
      481 gccccttgcc atctctaact cctcagtcac aagttacaat attcataaac catgaaaagc
      541 ttgtttattg aaattgctcc ttgtgacttg gagctaagtg acagattaca acctcttttg
      601 catcataaga ctttactcct tgaaaaacat tactaacttt tgatatgttt cttgcgcttg
      661 tttattgctg aaatcaaact gatattcatg gttcaaaaca gcatttttct ccgcataatc
      721 tacttttgtg actttcaccc ccgctttctc aaatacagaa gttacttctt ccatttgttt
      781 cgtaaacgta tttgtatttc catccgttaa gaatgtgttt tgcgtccaac gatccgcatg
      841 tctgaccaag gctaaacttt tgaccttctc tgattgttca aagtccttat tatcaaagta
      901 agcccatcca catttttcat aaagaaagct agagatttta tcacttactt gagtaagtgc
      961 agaaggatcc aacagaccag acaaagaaat aaacttcgta atctgcttgt tctccaaagt
     1021 cggatcaaga tgatcagctt ttttaatttc ttcattttga agtgctaaca aatattctcc
     1081 actaatttgt gcacctgccg aatttccacc aatcgaaata ttcgaccaat ctgcttgatc
     1141 ttttatctct tttttagctg cttgaatggc ttcgttcaat tggataactg gtgtaggatg
     1201 ttttgcttcc ggtgatcgtt cataatctat atttacgatg atttgctgcg tgtctgaaca
     1261 tagatattcc aaataatcac gacaatcatt tttatcccct cctacatatg cgcctccatg
     1321 catccagaaa agaatcggta attttttctt ttccactttc ggtgtgtaga tatccatatt
     1381 attatttttg tactttgaag gataggatat ttcttttttt atatcgattt gttcgatttt
     1441 ttgctggaaa tcatctggtc gggcatatac ttgttctttt tcacttgtgt caaattgttt
     1501 tctaacaagc aagctaatcg gctttggtgt gatttgatta atcaaaaata cagtgactaa
     1561 aataactacg atgataactg gtactgcaat cactattcgc ttgaaccatt tcattcattt
     1621 accctctctt tcttcgccaa atccatgtga taatcgtgct aacagataaa aatccacctg
     1681 aataaatgag aagcgaatgt ttcttttctc cagttttcgg caacattctt ttttttgcat
     1741 ctgtcttttt ttgtactttc actgtcatct gaatcggttg cgtatcatca cttgattgat
     1801 cctctcgagg ttcattcttt cccttattgt ctgcattctc tgtcggatct tgcttttttg
     1861 gtggagctac tgggattttt ttgtaataga aaataatagt tggtatcgtc tcattaaaaa
     1921 tcccttcagt cggtccttca acatttgcta attcgtatcc ttcgaatgtc ttttgctctg
     1981 tcatataatt ttctccataa gctccgtaaa gctggatttc gtcctggtct ttgagtagac
     2041 ttttattgtt ttcatccaca tactttacgt ttatctgatc atggaacact actttcaaaa
     2101 agtccgttac atgtttcgtg taggcagcat ggtcaacttc ctgtgccttt ccatgccctg
     2161 ctccttctac taataaaaga tctttatacc ctcctgataa ctcatataat ttttgcgcgt
     2221 tagacacagg tactacatca tctgctgttc catggataat caatttaggc aatgaatctg
     2281 acattccaat cgtaggtaga gcagcttttg tatccaaatg catctcctga tcaaaatagt
     2341 attgtttcaa aaggcccata aataaatctt catgctcttg atccccaatg atgtctaact
     2401 gattttttat ggcatccggt agtgcggggg ctactgtctg gctgtacgta aaacgtagtt
     2461 gttcatcaat tgatgcaaat ccgcaatttt caataattcc tttgacattg acaggaatat
     2521 ctttcgctaa tacagacatc atcgtagctg ctcccatcga accaccataa agtaaaatct
     2581 tttgagatgg ccgctctcga acttcttgat ttatccagta aaggacgtca tctgactcat
     2641 attgtccata ggtcacataa tcgcctccgc tattaccagt cgctctggaa tcaacaaata
     2701 ataaattata cccggcctta taaaaatcat tgtattcttc tgtaacgatc ccgttattcc
     2761 aattccctcg aaatccgcca tggatcatga ctgttttatc tgaaccttga tcgacataat
     2821 atgcatatac atccatcatt ttgccatctt gtttgtttag tacagtttga tgaagttgtt
     2881 ttgtatttgg atagaagcct gtgttttctg taggcgttgt cccatcatct gtacctccaa
     2941 gcatcgacat caacacactg ttttttgcaa aagcgatatc gtacaataga taagtagcaa
     3001 tcagatgttt gataatctct ttatctacac cataatcatt aacagcaagc tcagtgacta
     3061 ataactctat taattttgac ttgatgtctt ctgttggtcc attaagtatt tctgtcatct
     3121 gtttaccgaa gtaattcaaa tattctttac cattgaaatt ttcaccttta ccgatatcat
     3181 cattttctgt cccattctct ttgtcactta ctttagcaga agaggattcg tcgtttgaca
     3241 tttttgtagc tgttgcttct tctgtttgtt ggttatctgt ggtagcggtc gtttcttgat
     3301 ctgtgctagt aacagatgtc tcaaccgttg tttcagtatt tgtttctgtt tgtgaagtac
     3361 tcgatttcgt ttcatttcca gcatcagtag tttctgtggt ttctacctcc cgagattccg
     3421 attcggaaga aatgctctct ccatatactg tccattgcgg gacgcttgta cttacagcaa
     3481 ataagatgac caacgctccc tttaatactt gttttgttct caagctattc actccttttt
     3541 atgtttctag gagaattata tgtaaacgca aacatttttt atacaaaaaa taaatggttt
     3601 atcccataaa aatagaacag gattattttc tcattttacg taataaagca gaatggataa
     3661 accagtatcc attctgcttt ttttaaacga agaaacaact tattgctcca aaaatgtcag
     3721 aaatcgttta tcccaaattt tctcgtaaaa agcgattgtt tcctttgctt tcataaaagg
     3781 attatcataa gttgtcgttg ctcctctaca catcataagc tggtatccta atccatgagc
     3841 aaacttcaca gtcgtatcta tacaatactc tgcttgtgcc ccacataatt cgatcgaatg
     3901 acagtccaat tcagttaata aaacactcaa attagtctta taaaatgaat tcgcatgggt
     3961 ttttctcaca aaataactgt gctcaggaat agataattcc gacaatagct cccatggttt
     4021 tgtttgtggc actaattgag catcttcgtg ttgaacgaaa ataactggtt tcccaatcgt
     4081 ttcgtatgtt ttgattcttt tattcactaa tgctgtcact gtttctaatt cgtgtatcgg
     4141 cgaagaatca aaacataccc ctttttgtaa atcgatcact actattgcgt cggctgtgtt
     4201 tgacatactt gtgccttctt tcttttttct tgatttaata gtatttctta tctagctagt
     4261 tccattatac gtgacaacat tttcttattt tacacctcta aatggcactt ttcgactatt
     4321 tcttcatgtt attatttcat aaatacttag ctattggagg aatgtatgat gattaaattg
     4381 tatacaggtt ctggctgctc gtcttcaaga cgcgcaaaac gttggcttat cgtccatgat
     4441 ataccgttta tcgaacagaa cttaattcgt caaccactaa ccaaaaatga atttatccac
     4501 ttactaaaat tgacaaacaa cggcgtgacc gaaatgctct ctactcattc tattagttat
     4561 aaaaatcttg ttcacagaat agatgaactc agtttgagtg aactatatat tgagattcgt
     4621 acatcccctt ctttattgaa aaaaccgatt atctacgacc aatctgcaaa acttttgact
     4681 ggatttagcg aagataaaat caggatgttc ctttctaaaa ctaaaagaaa gcaagagctc
     4741 aacgaactct tgctttctgg tcatcttgtc gattttaaag aaaataatct ttttgcaatg
     4801 tcttaatttt ggcataaaca atctgctttt cttaattaac gaaatccaat ttttccaata
     4861 tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc gcccaatcat
     4921 tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca tatgcctttt
     4981 tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat gcactatatt
     5041 ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc tgtgttttac
     5101 catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa atatccctga
     5161 ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta ttaagctctg
     5221 gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc actggaaata
     5281 tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc ttttttgtcc
     5341 caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa tttttttctg
     5401 aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac gattcaatcc
     5461 agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc aaataggtgt
     5521 tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg tgattcatcg
     5581 accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct tcttttgtta
     5641 tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag tggccttgtt
     5701 tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct atactacgcg
     5761 tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt atacttgata
     5821 atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa aagtatcgga
     5881 tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat aaatgatagt
     5941 ttttcaaaat agtattaagt tctcttaatt ttctttgaat cgtacgcaaa cttatataat
     6001 tttttgacta agattttcta aggattgata agtacctttg taaagtgaat ccagtaaaat
     6061 ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa actgagaaga
     6121 aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt ctggatacct
     6181 ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg tgtagtttag
     6241 atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac aatagttcaa
     6301 aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag tacatactcc
     6361 tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca aatagattcg
     6421 ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat tcttgctctt
     6481 attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa acaaaaatag
     6541 atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa tattcactca
     6601 aaaatctatc gttttattca attttagatc tgttgctttt attctttatt tcactcgatg
     6661 gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac ccatccatgt
     6721 aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc tattatctgt
     6781 ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct gttttagcac
     6841 actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga taggtaccaa
     6901 atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc ctgtgctact
     6961 ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
     7021 gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt
     7081 gttttctgtg ctaatttcac tgctcgtact ctctgtactg ctttcaccgc tcgtgttttc
     7141 tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa ctgtccaact
     7201 gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa tcaaatcaac
     7261 tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta ttgtatactc
     7321 aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt tttctgaact
     7381 tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat tcacgctttt
     7441 gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa tccgatcagt
     7501 tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac tgattccaat
     7561 cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg ttttcttcat
     7621 ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata aaatgaaaat
     7681 gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta ttcgcttgaa
     7741 tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg atatcagcct
     7801 tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct ttgttttata
     7861 tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg atatagatgc
     7921 ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca gagtggagaa
     7981 aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt gtgtccaatt
     8041 ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga taagtgcgct
     8101 gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt taggattcaa
     8161 tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg gaccaaatct
     8221 aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc ccactgcaat
     8281 aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat ctaaccgata
     8341 cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc caacaacgag
     8401 acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat cttctattct
     8461 ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta aatgtccgac
     8521 cgtaccggca attgctccaa tccattcact atttccaata aacatatccg caagtgcatt
     8581 attgacaaga attcctaaaa caaaaccaac aaagactatt aaaacagatc caatcaaccg
     8641 aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc ttttctttct
     8701 tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt ttacgaacta
     8761 agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct tctgttagac
     8821 tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa ttatgtgcct
     8881 ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa cgattgcaac
     8941 gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag ttaaatcaaa
     9001 taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg tatacggaaa
     9061 agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa aactcggtcc
     9121 aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata agaaactctt
     9181 taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa cagataaaga
     9241 tctagacact gcttggaaag aattctatcc agaagttagt acacagatca ttctattttc
     9301 gactaaagaa gaagcagata aagcaaaaaa agaagcaaat gaaggagaaa atttctccaa
     9361 gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa aaatgaattt
     9421 tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta aactaaaaaa
     9481 cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc aaagctatta
     9541 tctagtaaaa atggtaaaaa aacaggataa aggttctaat aaagacaaat acaaatctga
     9601 gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg ataaaacaat
     9661 ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa aaaatatatt
     9721 caaataaaca aatttgaccc aattggggat gtggcaaaat ttgagaagtg atttttggaa
     9781 attccttctt atttttgagg caaaccactt ttgccacaac ttcatcgggg ccttttttta
     9841 tttgtaccaa aacaaatcac tggatttgtt acaatcatga ggatataatt taaaaaagtt
     9901 ttttatttct atcacatgaa tcaatgcgcc attaagtaga ttctttcatg cttccctatt
     9961 ttttcacttg ccgtatcgct tcaaaaacaa ctatcataaa ctcaaactcg actgaaaagt
    10021 tattttaaaa aagactaaaa atatcccttc tggacttgtt cttcttattc tgttatacta
    10081 gagaaacacg aaagatattt ttatgcgaca cttttcattt gtttttatga aatttattca
    10141 aaactggcgt aacttcagag ttacaaggat gtattagagg ttagggaata catcgtttaa
    10201 atgagaaata agtaatagtt agtaatagtc acccaaactt taaattaatg aatattgaat
    10261 cgtcagatag cctcctcgag ttttcgagaa ggctatcgtt tattttatgg agaaattgat
    10321 tcagaagtaa tcagcgacct tttttagttc accaatcgtt tctgcttgga aagtttgcac
    10381 ccaatcttct tggaagcccc tcactgcttt atcaatcgag ggcatctcta atacttgatc
    10441 tagtagctct ttacctaacg ttatcgctga gctccagatc gaatggctgt agtgacttgt
    10501 tcacaatttt tgaaactagc tgctaatatt tttgttggtc tttgcgtacg ttcgatctca
    10561 gtccgcaacg aatcaatcac tttcatagca tcaatgcctt gattttgcat cctgttataa
    10621 taaggtgcaa tataatctgc tccattatta atagctaaat acccttgtaa ttctgtgtaa
    10681 atagctgtac cggtaatcaa ataaccttct tttttcaact ttttgatcgc ttgaaggcct
    10741 gcttgattca ccggcacctt gataaacgta tcttttccca gttcttctat gataactcga
    10801 gcatcctcta tcatttcttc ttccgttctt cctattgtct gaatatggat aggaatctgt
    10861 ccgattattt ttttgatttc tttcatatgc gagaagaagt ttgacgctcc ttcttttttc
    10921 agaattgtag gatttgttgt tacgcctgtt aaattcaaaa actgttgaca agaataaata
    10981 ctttttgtat ttgctgtatc aagcatgatt tccatatagc tttcccccat tctgatcctc
    11041 ttgatcgatt ttaatgattg tcccctttct taaatgcggc atttccttat cttctacata
    11101 cattgttcct ggaagttccg gctcatgaga tccatcaaat tttatcgtga tatgtcctaa
    11161 ttcattcaaa ttagtttgga caacatctcc tactgctgtg attacataca tttgtttatc
    11221 aaaacagagc ttcatatcac aagttatttc ttttcttacc tgattcaatt caatcttata
    11281 acaataatct gttaatgttt ccggtgcttc atctccaaat aaaatcatta ttttttcttt
    11341 cctgaatact tctgcttcta taccaacatc tgttacaacc gtttcaaaga tcgtcatctc
    11401 t
//
LOCUS       DACCXW010000094        11154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCXW010000094 DACCXW010000000
VERSION     DACCXW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.095"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            116..1375
                     /gene="lysA"
                     /locus_tag="IVY71_002110"
     CDS             116..1375
                     /gene="lysA"
                     /locus_tag="IVY71_002110"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7986153.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1362..2162
                     /gene="dapF"
                     /locus_tag="IVY71_002111"
     CDS             1362..2162
                     /gene="dapF"
                     /locus_tag="IVY71_002111"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7986154.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2165..3046
                     /locus_tag="IVY71_002112"
     CDS             2165..3046
                     /locus_tag="IVY71_002112"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7986155.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3043..3759
                     /locus_tag="IVY71_002113"
     CDS             3043..3759
                     /locus_tag="IVY71_002113"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7986156.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3765..4460
                     /gene="dapD"
                     /locus_tag="IVY71_002114"
     CDS             3765..4460
                     /gene="dapD"
                     /locus_tag="IVY71_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7986157.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4476..5477
                     /locus_tag="IVY71_002115"
     CDS             4476..5477
                     /locus_tag="IVY71_002115"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7986158.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5474..6835
                     /locus_tag="IVY71_002116"
     CDS             5474..6835
                     /locus_tag="IVY71_002116"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7986159.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6795..7946
                     /locus_tag="IVY71_002117"
     CDS             6795..7946
                     /locus_tag="IVY71_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7986160.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7921..9090
                     /locus_tag="IVY71_002118"
     CDS             7921..9090
                     /locus_tag="IVY71_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7986161.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9265..10644
                     /locus_tag="IVY71_002119"
     CDS             9265..10644
                     /locus_tag="IVY71_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7986162.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10925..11154
                     /locus_tag="IVY71_002120"
                     /pseudo
     CDS             10925..11154
                     /locus_tag="IVY71_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3716 a         1948 c         2497 g         2993 t
ORIGIN      
        1 gaaatatact agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct
       61 ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac
      121 agaattgata attggcggag tcacagcaag cacattggca gaaaagtata agacacctct
      181 gtatgtgtat gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt
      241 atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat
      301 gctgcattta gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta
      361 taccgctttg caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac
      421 gccagaggaa ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt
      481 gatggaagtt gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat
      541 gcttcggttg aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat
      601 tgattcaaaa ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat
      661 aaaagaaagc agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt
      721 tgatgttact gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga
      781 ccaacctctt tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca
      841 gccgatccca gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga
      901 aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga
      961 agctggtaca acactataca cgattggttt catcaaaaag acaccacata aaaaatatta
     1021 ctttgtagat ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga
     1081 ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa
     1141 aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg
     1201 tgatctttta gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa
     1261 tcgagcgaca cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg
     1321 cagacaagca ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata
     1381 atggctgcgg aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat
     1441 tagccgcttc tttatgcaca aaagaaaaat atgatataga cggattgatc gccgtaaaaa
     1501 tcgatccgtt ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa
     1561 atggcattcg ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat
     1621 tcaacgtaga gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt
     1681 attgcagcgt agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa
     1741 cttatccaat aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt
     1801 tatttttagg aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga
     1861 gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact
     1921 ttgtagaagt agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt
     1981 ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga
     2041 aaatttcagc agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg
     2101 aggatgcgat acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat
     2161 gaagatgata aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt
     2221 agattatgct ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt
     2281 attagtttta ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat
     2341 ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac
     2401 gaataatacg aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc
     2461 acttttggtc atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt
     2521 tacagcaatt gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac
     2581 aggcatgtcg attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg
     2641 tttgaaagaa gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga
     2701 agaattcttc ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg
     2761 aagcggtgta ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa
     2821 agatacgcag gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact
     2881 gatccatgcc ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt
     2941 agacttacct tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa
     3001 acaattagct cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt
     3061 ggcagtggac agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc
     3121 cttcttttac ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa
     3181 ctgcttattg atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca
     3241 tatcaacttc cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata
     3301 aaagaacatg ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg
     3361 gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg
     3421 attgaaaaac atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt
     3481 caaacaatcc aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga
     3541 gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat
     3601 gtattgtttg ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga
     3661 atctttgctg caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat
     3721 tacaaattag aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa
     3781 tcatccgcta cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa
     3841 ccgagccaat cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt
     3901 ttggtgacta tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa
     3961 tcgaaatcga aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta
     4021 atgctcgtat cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg
     4081 tgatcatgat gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg
     4141 atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag
     4201 gagctgtctt agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg
     4261 gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag
     4321 tcgtggcagc aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa
     4381 tacctgcacg tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg
     4441 ctgcgttacg cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg
     4501 tgggggcaac cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc
     4561 cagtaggacg attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca
     4621 agaaaaagaa acacactgta caggaattga cagaaacttc atttgaagga tatgatttag
     4681 cgctattttc tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg
     4741 gactatttgt catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg
     4801 ttccagaggt caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt
     4861 caaccattca agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag
     4921 tcaattattc gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc
     4981 atcgtacgca aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga
     5041 ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa
     5101 atgagacccg taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg
     5161 tgccaatcga aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga
     5221 cggaagaaat tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag
     5281 cagcacataa atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga
     5341 ggatcagaaa agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca
     5401 tacgcaaagg agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag
     5461 cggaggtggc aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc
     5521 tcatgaagag caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt
     5581 gatcgttgtt tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact
     5641 ttatttgatt tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat
     5701 ttgtgatcgt ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga
     5761 actagcacgc atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag
     5821 tagaggtgaa tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga
     5881 cgcaaaagac ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca
     5941 ggaaatcaca aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc
     6001 gaaccctgca ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat
     6061 actggcgaag atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat
     6121 gatggctgat ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga
     6181 actgagagag atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt
     6241 tcaagaggct gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac
     6301 aaaaatatcg aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa
     6361 ggattttctt tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg
     6421 gaaagcaatg agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat
     6481 cgacaatatc ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac
     6541 gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt
     6601 gatctctgtc gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc
     6661 gatattgaac aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa
     6721 tctgatcatc ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg
     6781 gatggtgaat acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg
     6841 ggtttcatga attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcgaggccg
     6901 ttatccatga aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa
     6961 cgattgcttt tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt
     7021 ttgcttctaa gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc
     7081 ttttagggtt gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac
     7141 tgatctatca gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat
     7201 tgttggataa atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta
     7261 agggagaagt tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta
     7321 tcattaaagg ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg
     7381 cagctgtacg atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga
     7441 atcatctaat gaaatttggc caaatccaag gtggaacgat ccgaaatgtt ttagctggag
     7501 aagttattgt atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga
     7561 ggggattaga ccatttagca gaacagtttc actctacagc tactatttct tttcgctata
     7621 atgacggcta tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa
     7681 acaaactaca tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc
     7741 aacaatatcc atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc
     7801 cgacgttcga ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc
     7861 tttttacaga aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata
     7921 atgatgaatt tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata
     7981 cgacaattcg atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa
     8041 ccggattttc caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac
     8101 ttttctcact ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt
     8161 cagcaacagc gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt
     8221 gcaacggaag cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt
     8281 attccagcgc ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg
     8341 atcccgatag atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca
     8401 tttgaacaat atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact
     8461 gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg
     8521 gtatttgtta taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc
     8581 atagctactt atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg
     8641 atgactggtt ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt
     8701 aaagtccatc aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa
     8761 gcgctaataa atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga
     8821 gactatctgg tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca
     8881 ttttacttgt tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta
     8941 gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga
     9001 gaaggttata tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa
     9061 cggatcaaag actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt
     9121 aattttgcgg tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc
     9181 gtttcttttg attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa
     9241 agaatattca aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct
     9301 catggagggc atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc
     9361 acgctatttg aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta
     9421 gaagacaagg taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa
     9481 tccaatgttc atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca
     9541 gtaacggtta ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt
     9601 ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc
     9661 tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct
     9721 attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc
     9781 cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat
     9841 ttagatgctg agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt
     9901 ttgacaaatg caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca
     9961 agtgaaaaag aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca
    10021 gaatggttaa aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac
    10081 ttgcaaacga atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc
    10141 ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat
    10201 gtagtaaaac atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc
    10261 tctgcattaa gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca
    10321 gcaaaagcag gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat
    10381 gtaccagaag aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc
    10441 catcaattgc taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta
    10501 ttggcactag cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc
    10561 ttccagccag gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta
    10621 ggagaagaac catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc
    10681 tgccttgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg
    10741 gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact
    10801 gtgacacgat ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt
    10861 cggtattttt tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt
    10921 tggatggcaa aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct
    10981 taaaattcgg gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa
    11041 ccgcggtttc tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa
    11101 agtaacggca tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DACCXW010000095        11097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCXW010000095 DACCXW010000000
VERSION     DACCXW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.096"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            387..1826
                     /locus_tag="IVY71_002121"
     CDS             387..1826
                     /locus_tag="IVY71_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7986163.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2121..2339
                     /locus_tag="IVY71_002122"
                     /pseudo
     CDS             2121..2339
                     /locus_tag="IVY71_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2588..2803
                     /locus_tag="IVY71_002123"
     CDS             2588..2803
                     /locus_tag="IVY71_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986164.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3020..3276)
                     /locus_tag="IVY71_002124"
                     /pseudo
     CDS             complement(3020..3276)
                     /locus_tag="IVY71_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(3463..4374)
                     /locus_tag="IVY71_002125"
     CDS             complement(3463..4374)
                     /locus_tag="IVY71_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7986165.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4393..5397)
                     /locus_tag="IVY71_002126"
     CDS             complement(4393..5397)
                     /locus_tag="IVY71_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7986166.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5394..7517)
                     /locus_tag="IVY71_002127"
     CDS             complement(5394..7517)
                     /locus_tag="IVY71_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7986167.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7522..9969)
                     /locus_tag="IVY71_002128"
     CDS             complement(7522..9969)
                     /locus_tag="IVY71_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7986168.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9956..10345)
                     /locus_tag="IVY71_002129"
     CDS             complement(9956..10345)
                     /locus_tag="IVY71_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7986169.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10405..10908)
                     /locus_tag="IVY71_002130"
     CDS             complement(10405..10908)
                     /locus_tag="IVY71_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7986170.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10921..>11097)
                     /locus_tag="IVY71_002131"
     CDS             complement(10921..>11097)
                     /locus_tag="IVY71_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986171.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         3237 a         2330 c         1997 g         3533 t
ORIGIN      
        1 tatttagtat aatcctatgg aattgaatat catagcaatg tatttgaaaa gcaaattgga
       61 aaacaaatag gtgcaacacc ataaatatga aaaaattggg aaaaagtaga atggaaattt
      121 gcttacgtct cacgagatag ctcacactat taatctgaag aacaatgaag tcgactttta
      181 gaaattgcta gaagaaagta aagcgaatta tcactagtta ctatagagta agttcaaata
      241 aagcatctcc aacttacatg gttacatgag gatatcaaat cgaaattact caagcttcct
      301 atggctagag tcgcgagaag ttgttgggca gtagacatag gttatattgt tcttagctac
      361 acgatcatta gaaaggaaat cctattatgt atagttatca agaagatgca aaagtccaaa
      421 aaaatcgctg gtggattttg attgctatat ctatgtttac cttcatgtcc acattggatg
      481 gcagtattgt caatattgca ctacctacca tctcagatga tatgggcata ccaatgaatc
      541 aagcggaatg ggtagtgtcc gtttatctga tgattgtctg ttcttgtttg ttgctgtttg
      601 gtaaaattgg cgatagccgt gggaaaatca aagtatttcg gattggtacg ctgatctttg
      661 tcttgggatc gctgttgtgt gggttcaatc attcgctagg tattttattg ttcgcacggg
      721 tggtacaagc cattggtgct agtatgacta tggctacaaa tgccggaatc atcacggaag
      781 tctttcccat acatgaacgt ggtcgggcat tgggagcaat aggtgcgttt gtttcattag
      841 gctcgattgc tggaccaggt cttggcggct tgattttgtc ccaattttct tgggcgtaca
      901 ttttttggat caatgtaccg gtgggaatcg taacgatgtt gattgctgaa aaattcttac
      961 caaaagatat caccatgagc ggccatacaa tcgatggagg gggctttgct gccattgcgg
     1021 ctacgattat cagctttttt ggagcaatct ttttggggca agaaagtggc tttttccatc
     1081 cagtccccct tgtactgttc gtaatttcag tgattgcctt tgtcagcttt ttaaaagttg
     1141 aaaagaaagc agcctctcca ttgatttctt tcggtatttt taagaacaag atttttacca
     1201 tgagcttgat tacagccttg ttgatttttt cttccaactt ctttgtcaat gtagtgattc
     1261 ctttttatct gcaaaatgct cgcgggttga gtcctagcta tgctgggctg ttgatgatgg
     1321 tctttccttt gatgatggtc gtcggggcgc ctttaagcgg ctacctgacc gaccgggttg
     1381 gcccggagat tttggtatta gtaggcttgg gcttattgtc ggtaacccag gtcatgtata
     1441 tgtttttgtc agtggactct cctttgtggc tatacattgt cgctactgcc atcatgggtg
     1501 ccggcaatgc cttgttccag tcccccaata atacgatggt tatgagctcg gtcactatcg
     1561 aaaacttggg actggcaggc tcgatgaatt cctttgcccg aaatttgggg atggtcagcg
     1621 ggatttcgct ttctacgacg gtcttatatc aagcgatgtc tgctaagatg ggccagcaag
     1681 taactaacta tatcgcaaat cggccggatg tctttttata tggaatgaag attacctttt
     1741 taggcagctt tgttttgtgt tttgttgcct ttttcctgac tatctggcgt atcaaaaaac
     1801 aaggaggtat tcagaaaaat gtctgaaaaa accactgttg aagaattttt ggaggcctgc
     1861 gtatgggtga gtgggatttc catggatgat ggaagatgaa gtgaacttct aaaagccgaa
     1921 aaactcaagt attatcttca aaatactagt gctcaatgag atgcttgatt tgatcacaag
     1981 aagactgcga aagccgctgg caaccgcaag ctaggtatcc caccgatgga ctacgtttta
     2041 gaggctactg gttatgaata cggagccaac gctctcatct gcataagaat acatcaccca
     2101 gattatgtaa agattttcga tacttctgtc aaaaaatcat taagatagat aaatgatttc
     2161 cgtctagtca aattggttca gaatgtagac acacagatgg taagaagtca ctcacaattc
     2221 gagaatggac ttgtcctatt tgccgtatac atcataatcg aaacgtcaac gatagtatca
     2281 atattttgac caagggctat gaatacaagc attgccttag aaaacatcaa gaaccttagg
     2341 aacaacgggg atagcttggt taataagaga taccgctgtt taggcttgca agtatactct
     2401 tttatcaaga attccgtggc tttagtcatg agaggcttaa tactatgaaa attttcttta
     2461 gcatttttga tttaggttcc ttttctaatt ataataagat gaccactaca gtaacctact
     2521 gaaatatata aacagccact gtagtaccat tttcaaagta gtctatgaga ggagaatctt
     2581 cattcttatg aacgctggaa ttgcggatat gaatttaata aaaaaaaccc ttaatgattt
     2641 tacttccaat tcaatatcaa aaggtactgg tatcaattta agcacaataa aaaagctgaa
     2701 aagtggtgaa agatcagtgg aaaagctgaa tcttttagat gctataaaaa taacggaatt
     2761 tgctatgaaa aatggaaaag ctgagattga aatatggaga taattatcgt ctagtgaatt
     2821 acttgccact tagctgaatt taacgatcct tggtgcttga agggggagat tcttgggaat
     2881 agagcatact tgcaagccta tgtctttact aacaacgata tctcttatta accaagctat
     2941 ccccgtagtt ctaaggcttt tacttttatc taagcaataa tcgtattcaa aggaaatttt
     3001 tgacccttag aaagtctcat tttatcagac gtgagcttag gcattgctga attttgttaa
     3061 ttcggtttta ggttgtgctt tgagcataat atgaaagtat tctttatcac gatttcattc
     3121 atctaaggta atgtgatgtg attctgaaat tctttcaaag gcagtttttg cataagctga
     3181 cattacatca tcaatgacat gtttacaatg tttcactact aaaataaggg ggtaaccaag
     3241 aagaaaaact gaatgattat ttgtatctaa ttccatggta tatcaaccat ttcctatata
     3301 gactgattat gacatggatt aaaataaaaa ggcactatgt cttaattgtc ggttttcgag
     3361 ttaccgaatg gcattcatca cccacctata gaggatgggc gacttctgcc tgaaataagt
     3421 tacactactt ataacttcta ataaaattag attgagtcat tattatttaa catcaacgat
     3481 tttccagttt ccattttttt gtaatactaa tttgtactga aagtagctag tggtttttga
     3541 tacattatca aggtatttta tctttgtact cactagaaaa ttttttccgt tttgtagata
     3601 aactggattc tctaactccg aaaactttaa gtcaatattg attggtcgca tcgcaccatt
     3661 ttcaacataa tactccaatt ctttttcaga agctgatgga taaagtttaa aaaatgtttc
     3721 taaaaaatcg gtgatttctt ccgttgtttt ggcatcaatt tctgaatcgt tttctacttg
     3781 ttttgcttca tattttgcct tgtctggctt tccaatcatt gttggcaaac tagttactaa
     3841 atgattcctg ttttcatctt caaagattgt cactcgatat gcagaagaaa ttgattgcgt
     3901 cttttttccc tctgtaatat gttgctccac taaaaatgaa acgacaaatt cttccgagtg
     3961 tttttcaaca tctagaattt ttacagattg aacttcggaa ctcgtgggaa tatctgctcg
     4021 aaccatatct tggattaaag cgagaccgtt ttctgtaagg tactgttcga gattactcat
     4081 tctcttttca attgcttctt tgttattttt ccatgaaaaa tattctttcg caaaatcctc
     4141 tgtaaaactt tcaacgccag aagtattcac taccttctct tttataacca ctttttcatg
     4201 agttgtatgt tgatcaatgg cggtgaaatt tttataaacg ccaaaagcaa gactacaaga
     4261 caataaaagc caaagggcaa gcaccatttt tcgatgctta cccacggata caactcgttc
     4321 ttttttcttt tttgggctct tttccttttg attccgttca atttttatct tcattctttt
     4381 tcctcttttc tcttatttgt atcggccagc tccgataaga tgctgccgcc aatagttttc
     4441 cgttagattc gtccacccca ctggatctcc tgcatgaaac atgcgattct cccctgcgta
     4501 aatgcccaca tgggtcacat aagtaccggc atcataggtt gaatgaaaga aaactaaatc
     4561 acctggtttg gcatctttta gatcaatatg agtcatggca tcatattgat cctgagccac
     4621 ccgaggaagt ttcaggtcaa ctttggcata acaccattgg gtcaagccac tacaatcaaa
     4681 ccccgtttct ggacttgagc caccaaacac ataggccgtt ccttcatact taaatgcctc
     4741 atcaaaaatt tcttggacta ttttgtcatc aaactgagtt gtagttaggt attgcttcac
     4801 taataaacag taaaacatat tgccataatt ataccgccac ccaccatttt ccttaatcgc
     4861 gattgggttg gggtaatcga ctttttgacc acctgatttt tcctttgaaa aagattcagc
     4921 taaagcaaaa ctatgcttct tcccgttttt cccgacatga tctagatacc cgccaccata
     4981 gttataggcc tgaataatac tatcttgatc acaccctagt tctttggaac gcttaaccaa
     5041 agacgcaaaa tagagacagc cttgtttgat ggatgcctcg gtacttaatg aattaggtgg
     5101 taatccggct gactcagaac tttgcataac gtcttccatt gtgccaccag attccacttg
     5161 aataatcgcc aaaagaattg gaatatactc tgttatatcc gattctttgg catacttttc
     5221 caccattgca cggtgtttca atacttcttc tgagacacta gttccgccag gagaagtgcc
     5281 agaactacca tttccctcac tgtcttcacc aaacaacaaa cccacacaca tcaaaatacc
     5341 cataaaagag atggtcagaa aagttaatga aataacaccc actttcttca agttcatttt
     5401 ttctcacctg accttgattt tggacgatgg ttcattcttt tttgtacttt ttgattggtc
     5461 tgctttggca gagtggacct ctgtttcact tgttggaaag aatttttctt acttgctgat
     5521 tggcttttcc cttttgaaat gatttcttga ctagttagtt ttcgtttgat ttcgggattc
     5581 agatttgctc tcacaatccg ctttggtgct tcttttttca gcatcatatc tttctgaggt
     5641 tgagctgctt tatctattgg aatattcgcc cttctttttc cctgacgatt tgttgcttca
     5701 ctcagtatct tgcgtttttc gtccattttc gcatttcttc gttgtcgttg tttttctcgc
     5761 aaatccactc gatccgcttt ttcttggaac agtccgcgct tgaattcttc tggtgctttt
     5821 ttcgactttt cgattccttt atggagattg tatttcaaat tcgttggcgt attccttacc
     5881 tgatccttta cttgtttagt cttatctttc accaaagctt gggtatccaa aactttgccg
     5941 atttttctcc cagccaaatt cattcgggat tgtttcttat tatttgaaac tggattctcc
     6001 ttagttaagt cagatagtat ttcgtgatcg ttcggtaatc gtgaattctt tcgtagagaa
     6061 gaaccggatg gcttaaattt ggattttgat tgttcctttg ccaacttatt tccgactgct
     6121 gcaccaccac caaaagccaa agctcgtccc acatttcgat tgagttgccg caattttcga
     6181 ttcattaaca tttgcggttt tcgcatgacg cgtcttccca ttgactggct atcattactc
     6241 tggaggttga acatactcat aatgtcgccc aatttgaaat agataccggc aaaggtcaca
     6301 atttgcagaa aagcaaccat gaaaaatgga taggttgccg aaatattgaa aaacatcgtg
     6361 gagatgctaa atgcagtcgt aatcaccaaa gtaacacccg ctcgcatcat aatcgtattg
     6421 aataaacgaa taatcgcctt ctttcctaaa ctttcataag tcggcaacat ggacaataag
     6481 aaactaattg gcagaaacat cgcaaagata atgaagagaa tttgcgagaa caacataatc
     6541 cctgttagaa gaaaaacaaa gaaggaaatg ccaatattga acaataccaa aaagaccacg
     6601 gtccctaaac ggttcatggt cttggtaata ctgagattcg cattgtcatt gtcttcaatc
     6661 tctgccttga ccgcttcttc ccgatctttg cctttgtttt catctggact cacagataga
     6721 atcttgttga cccggtcttc accaatttct tctatgttgg aatcatcaaa ttgcagtagt
     6781 aaccaaggtt gttggacttg aatcgaaaac aaactatccc gaatcaaatc cacactatct
     6841 tttccttggc tttctgaatt gggtacaacg atctcggttc caagggtaag ggccgcttca
     6901 ctaacatctg agctaaagtc attgattttt gtgatatagg taggcgcata agcaatgaac
     6961 gaacccgata gtagaaaaat catcacaaaa ttaatcacgg ctcgaactgc tttcgtggtt
     7021 tctcgtttta ataatccggt atacgccaca tacaccccca gagctaaaat catcaataat
     7081 agaaacccaa aataaaatcc ggaggtctgg agtccatttt cagtaatacc agccaatgtt
     7141 tggatattct ttccaatact ttcagccgta tccgaaatga aatctaattt gtaagcctct
     7201 tgaaccacat aacccgtggc gttcgacaaa tacaaactca ccgtccaaat gaaattagta
     7261 atggcataaa gaccatacat cacgcttttc cctaagccat caccccaatt ccaaggcaac
     7321 caatcccaag agctatccac aaagaaatct agttgatagt tgttcagtga atatttggaa
     7381 tatagatttc ctgcttcaac tgtatcatca actagccctg tcgcctcggc taccgtacca
     7441 actaaactca atagaactat cagagtgagt atgctaataa aaacgattcc cacaatccga
     7501 aaaagtttct ttttcatccc ctcactccat tctctcttga acaggtggac gggtatcaaa
     7561 ggcatggaag agttcttcaa agactggatg tacctgaacc acgcctaccc gaccatacaa
     7621 atcctgcatc aagcattggc cattttccaa atctcgtagg cgtttttgat tgctttcatc
     7681 ttctcgatcc aagccaaaga attccaaggt tttcttgatt tcatgaatat cggtactgcg
     7741 aaacgcaaac ttcaacccga tattattctt caagcgttca tccagtaaat catcactatt
     7801 ttgcgtgaca aagtaaaccc cggcattcat agcccgccca gcacgaacga gcttattcga
     7861 taatgttttc ccttgcgcta cttgaagaaa actccacgcc tcatctaagt ctacaatttt
     7921 gaagatttct cgattactat ggataaagtc caaggcaaaa gtggaaatga cgattagcat
     7981 ggaaacacta agcaattcca tggtggtata ttcttcaaaa gtggtttctg catctggtaa
     8041 aaccaaatcc gctacttgaa tgatatttaa ctgcctatct aaactaatcg actgggtaat
     8101 cgaaccatct gagaaaagca aatgggcaaa atcataatcg gtaaaggact cgatgtgatc
     8161 cgcgatattt tccgcaactg gtgagccttc ttttcgaagc tcattaatta cttgtaataa
     8221 tccccgttga ttgctttggg taaccgtccg aatggcctta cgcaacactg gaaacttctc
     8281 cccatctcga ctagaaattc ctgttaaaaa ggtcagaata tcaattgcta gactttcaga
     8341 atccttcttt ttctccataa tcacataagg atcaagtaat ccttgatttt ttggcttact
     8401 agttaagttg agaatattga tttcatccgc aatttctggc aaggtttctt tccagccacc
     8461 ccgttcccct tttggatcga caatcacagc ttgtccacca aaaaggacgg cgtaataaac
     8521 taagagattg ttactaaagg actttccgcc accgagtgaa ccgaggaatg ccgctgctag
     8581 cgcattggta actgaatcct tgaccccttg tgaggccaat gccggtttca ggtaaacatt
     8641 gcgccccgta tctacgttat aaccaaagta aatgccttcc aactccccta acatctgagt
     8701 ggcgccaaaa ccaagtccgg cgagaaaatc agacgtaaca tactggatat aatcattcat
     8761 gtatcgctta ctagaaggta agaattcttc atgtagcccc atcatatcgc caaaaggccg
     8821 gactagtttt acattggtat catcataaaa atctagcact tcatcacacc ggcgctttag
     8881 ttcatctact gattcggcac ttactcgaac cacatagctc agtttgtaca tggattcttt
     8941 ggactgatcc aaggtagttt ccaattcatc tacggaatcc aatgcatcta agacattgga
     9001 atttgtttca ttgtcagatt gataggcatg gttgtccaag tcttttaatt cctttttctt
     9061 attgcgaacg gtagcaagtg cctttttgtt cgtcacaatc tccacattca tactagtgtc
     9121 aattgggaat gtaaattgtt gttgctggta atagaaaagt tctgaggagg gaaactccat
     9181 ttcccccaca attgtattaa ttgttaagta cgctacatat gattcatgat tctcatgttc
     9241 catgcataag taccggggct tttcttcaat caaacaacga ctgggacgta ataagtcgta
     9301 acgtttcacc aaagtttccg acttcagctt tttctttggt agctgaaatt catattcttc
     9361 aaatggtgta cccttttcgc catagatatg ttcaatcaaa tacatcagat cactcggtgt
     9421 gactcgtctg actttaaatc ttcgagccaa tttactttcc attagttttt caagcttggt
     9481 atatcggcga atttcttcat tggataagga aacaaaatcg cccatcaaat gatgattcac
     9541 tccatagaca aattcttgaa agccggagaa gaaagatttc ttgagattct ttagattgac
     9601 ctcttcatct gtcgcaatca gtttgaagcc aataaaaaag cgataatcaa tctgactatc
     9661 gccaatcatg gatactaatg cctctgtctg taaatcaatt cgttgtttcg ctacttctgt
     9721 taatcgcccg gtaatttcct tttttgatcg ttcttgcgtg gcacgaatac tactttctgt
     9781 ggcaatttgt aaggcatgaa tcttgccttc acgattttgc gcaatcaatt gacgaaagtt
     9841 atcgtggact tgatatttct gttcaggtga caaaaaggag taattgtaag gtattaactc
     9901 atagtaggca aaacattctc cctcttgatt aaagacaaga ttattttcaa tatacttaat
     9961 cgggtacata gttcacactc cttaccagag taatgggttc gtcccatcgt ttcttctgtt
    10021 ctttgacttt ctttcctgcg aaagtgacct taaatcgtaa gtgatagcta ataaaagatc
    10081 gtaaaaatcc caatggtttc ttaccgtcaa acgttttttg cgacatgaac caagtaactg
    10141 ccaccggtat acctaagtat tttaaaaagg ccccctctat catagatagt ggaggcacat
    10201 gggcaaatac gatgacaaac agcaaggata aaacaaacca agccatctgg ttaaaagtca
    10261 caggaaaagg cagttgaaag tcattaattg cataaatgac cttttcaact gcccaaatac
    10321 tggtgtagct ttttattttc ttcatcaaat cattccttta aaaagaagga tagtttagaa
    10381 atcaactacc cttcaataat tttgttaata tggatactca aaaacacctc gactcgttac
    10441 caaataattg cctccaatct ccaagtctct accacacgca tcaaaatcaa tatacgtctt
    10501 caagtgttct ggtacttctc caaaatcacc agcttctaca atcaagaaat atgctacatc
    10561 actcatatca tcgcaatctg gataatagat aatatcatcc acatgctcta ccatctcctc
    10621 aaaagaattg aagaaagcat gttgaatctc agaagcaact tcaccaactg gcgttccttc
    10681 aagttcctcc gctaagccac acaaccgatt aatttcctca attggcgtat actcatcaat
    10741 ctcaaaggga agttcataat catgaattgc atattcctcg tattcgtcat ttaacccaat
    10801 tcgttctttc acttcatcaa aatcgacagg tggggtaaac cacgccccca ccaattcacc
    10861 ttcgttatat ttcccgaggt tcgcaatata aacacgcatc tgttccatcg tgcttccccc
    10921 ttacgcaccg acaattcggt taaacaaact gagtaagaca tccttcaccc cagaagcatt
    10981 aaagaccaat ccgaccgcaa tcaaagcaat caccaaaaag ccaatcagtt tggaaaactc
    11041 ccgcttaaat cctaaaaaca aaccaatgac cacaattgcc agtaagacta aggattg
//
LOCUS       DACCXW010000096        11037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCXW010000096 DACCXW010000000
VERSION     DACCXW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.097"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(130..777)
                     /locus_tag="IVY71_002132"
     CDS             complement(130..777)
                     /locus_tag="IVY71_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP7986172.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1056..1559)
                     /locus_tag="IVY71_002133"
     CDS             complement(1056..1559)
                     /locus_tag="IVY71_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7986173.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1682..2446)
                     /locus_tag="IVY71_002134"
     CDS             complement(1682..2446)
                     /locus_tag="IVY71_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7986174.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2553..2828
                     /locus_tag="IVY71_002135"
     CDS             2553..2828
                     /locus_tag="IVY71_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7986175.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3505..4455
                     /gene="yidC"
                     /locus_tag="IVY71_002136"
     CDS             3505..4455
                     /gene="yidC"
                     /locus_tag="IVY71_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7986176.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="IVY71_002137"
     CDS             complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="IVY71_002137"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7986177.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5605..6063)
                     /locus_tag="IVY71_002138"
     CDS             complement(5605..6063)
                     /locus_tag="IVY71_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7986178.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6060..7523)
                     /locus_tag="IVY71_002139"
     CDS             complement(6060..7523)
                     /locus_tag="IVY71_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7986179.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="IVY71_002140"
     CDS             complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="IVY71_002140"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7986180.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8598..9347
                     /locus_tag="IVY71_002141"
     CDS             8598..9347
                     /locus_tag="IVY71_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7986181.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
     gene            9614..>11037
                     /locus_tag="IVY71_002142"
     CDS             9614..>11037
                     /locus_tag="IVY71_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP7986182.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDS"
BASE COUNT         3417 a         2268 c         1893 g         3459 t
ORIGIN      
        1 aaagaaaggg gctatcccca gactttagta ggcgtcatta gctatctttc gttagcattt
       61 aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa
      121 acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt
      181 cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata
      241 cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gacacattcg atgttttctg
      301 acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa
      361 tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat
      421 gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt ttttcacttt
      481 tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgga cgatttgctt
      541 cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt
      601 taaaaatgta tcgatttctg gcagttcgtg ttctaatatg tctcgaatct tttcagctgc
      661 tagtgcctga actgtttgta ccgtttttgg atcattgaca gatgagtacg catttaataa
      721 ctgatctact tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat
      781 tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa
      841 agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg catcttatca
      901 tcaccccctt taatgttcat tcttctatgt acatgattca ttcgataaat aaaagtaaac
      961 cgtatcttga acagatagtc aactgcaaac agaaaaaacc gagaaaatca gccagatttt
     1021 ctcggaatat agtctattat tattggtttt acctattaaa tcgaggattc tttcccaaag
     1081 taacgttgcc atctcttacg catacttgca gacatgggct gagccgcttc tttgactgcg
     1141 cagtatttgt ccacgaatgt cacaatatat ccttctttat acttaggcgg tgcgatcgtt
     1201 gcaccccaca tgtgcttcaa aatgatatct cgctcaagat ccgacaaatc ggtgattttt
     1261 tctgcattct ttacagcaat tcttggatgg atatacgcat gtgtcccctc gtcaaatttc
     1321 gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta
     1381 gcattgccac cccatttttt tgcgagcaga taactatagt aagaaacact gatggaatgc
     1441 tccaaacgag tggaatgaac atgctgtgtg taattcgcta attttttcac tgcttctgtt
     1501 tctaaaagat cttgtacata agacatatat tcttcgtctt cacgccattt ttctgtcata
     1561 attatcgatc tgccaccttt ttgaatttca ttttcttctg aaattgatct aagtatacca
     1621 ccagtgttcg gattattcta gtcgctctag ataaaatagc taaagcttaa tagttcctta
     1681 atcataaaga aaaatgataa agaacgatcc ctgctgcgac tccgacattc aatgattccg
     1741 catttccttt cattgggata tatacgttct gatcagtcaa agacaagatt tcctgactta
     1801 ctccctgacc ttcgtttccc atgattatag cgaaatcctc tgttttttct actttgtcta
     1861 atgcatgtgc ttcttcattt aattctgtac cgtacaccgt aaaccctttg cctttcatta
     1921 cagggattac atctgccaaa tctttttgta acacaggcaa atgataattg cttccctgca
     1981 tgcttcgcag cacctttgta ctataaatat ctgccgttcc tttacctaat aaaacaccag
     2041 ccataccaaa cgcatctgcc gtacggatca tcgttcccac atttccagga tcctgtacat
     2101 tatctagtaa aagccagcct cctgaaaaat ctggcgtatc ttcagatgtt aacgtcatca
     2161 ctgctaagat cccttgcggt gtaggtagat ctgacatagc ggtcatgacc tcatctgaaa
     2221 ctagaaaagt agaaatctct ttgtttacag ccagccagtc tccccattct ttcatacctc
     2281 tctgtgtcac aaaaagttct tttatttctg cattagacga cgcagcttct tcgatcagat
     2341 ggaacccttc caaaagatat tctgcctttt cttctctgta tttcttttta tgtagttttt
     2401 ttaattcttt gattcttgga ttttttgctg attggatctc tttcacattc ctcacctcaa
     2461 ctgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt
     2521 gatttataaa acaggaaaga aaggtgacga acatgcgcaa agttaaaatg aatgtccaag
     2581 gacgtgtcca gggcgtggga tttcgctata tgaccaagat ggttgctgat cagttaggcg
     2641 tgaccggaac ggtaaaaaac gaagacgacg gctcagtcac aatcgaagca atcggaaatg
     2701 acgacatcat ccaaaaattt atcgaagaag tcaagaagtc tccgagccca agtggacgag
     2761 tgcagtatgt ggatgtccaa gaagatccga tgattgagga acgaaagaaa tttgatgtag
     2821 tgggataagg ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt ttgtgaaaat
     2881 ggctttccac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     2941 tcgataaggt tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca ttttcaaaat
     3001 agcttctcat attttgaaat gtaatgactt aataccgaga ggcagctgat aaaacaccgt
     3061 caataaggtt gtgaaaaagc tggtctgcat caagcattaa ggactggttg tgataaatag
     3121 ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt tgaacaccgt
     3181 cgataaggtt gtttttgagg cgctttgcct caagtattaa agacaggtag taaaaaagct
     3241 gatctataac ttgcaatgaa tttgcttaac ccatattttc cagacagaac aaaaaagttt
     3301 ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcta agtatttctt
     3361 tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaacgaa tatttatatg
     3421 aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa
     3481 aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga
     3541 cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct
     3601 gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat
     3661 ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac tgtgatcgtt
     3721 cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat ccaatctgaa
     3781 aaaatgcaag cgttaaaacc acaagttgaa gcagcacagc aaaagctaaa agctgcaaca
     3841 acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg cgaaaatgga
     3901 ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct
     3961 gccttgtatt ttacggcacg ctacacagaa ggtatccggg aatcatcttt ttacggcatc
     4021 gatctaggct caccaagtat ggtgcttgtg gccatcgcag gggtcgctta tctgcttcaa
     4081 ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgctg
     4141 atcgtctctc ctgctatgat cgtcttcatg tcaatcagtg cgccagccgg tgttacactt
     4201 tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg
     4261 aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc
     4321 gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa acaagcaaca
     4381 aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc aggaaagcag
     4441 cagaagcgga agtagatagt aaataagagt gtaataaaaa ttcattaaca gttttagcaa
     4501 ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt cgatattttt atttaagtga
     4561 atgtttaatt tgaatcatcg attgatcgct atctaattgc agcagcatct ttttatgaag
     4621 aaacaacttt ttcttatcta tcaacgtaga caggtgttgc tgtttcttct aatagtgtac
     4681 ttaattcttt aatattttgt tttccttctt ctaacatcca actactcgct ttttcttcgc
     4741 cttcggtaac aaaaggtgcc actcccttcg cccatgttgc tcgcccacat aagacaccat
     4801 tgaatgtcga acctgattgt tttgcaaaac gtaatgtatc ttgaaatagc gtagcattga
     4861 ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca tagaaatacg
     4921 cggatgcctc tgcttgacta taacaagtct cttgcccata tccttccaca aatctcatat
     4981 tcactggcac ttctactttc aatacatcca cttgataacg ttcttttgaa aattctcgca
     5041 tggcttcaat aactttatgt ggttttactt ttgcataggc aagagaagac ggatcgccaa
     5101 tagttgcatc ataagaaaga atctctaaaa agaaaggtat gccttctgct tcgcattctg
     5161 aaccaatccg ttctataact atttgttttt gcacattaat ctctgctggt tcatcaacgt
     5221 catagtacaa taaaaattta caagcatccg caccagcttc ttttaacctt ttcaccgacc
     5281 aaatattcaa taaatcaggt aatcttcccg gtattttcgt atcatagccg gttttttcat
     5341 aagctagcag taagccacat tctttctctc gaagcgctgc tgcaggcagg ccgtattctg
     5401 gatcaagtaa aatagctgag gcatagggag tcaggtattg agaaaccatc tgtttaaatt
     5461 ggatgatgtc ttctgatgta gcttctttat attggttgat cattttcttc aaagcaccac
     5521 gctggtcgat tgctaatgcg ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt
     5581 cctgtttttc tatactcatc ttcttcatag atcttcacct atttctttag ttaatattgt
     5641 ggctatttct gtttttgatt ttgcttttct caactgttct gtaacctctt ttcgcatcaa
     5701 tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg aatctggaat
     5761 aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt caattccttt
     5821 gttcagtgtg gcgataatca tactaggttc gttgattgtt tgagacttcg catgagggat
     5881 agcaaaacct gacattattc ctgtagtccc ttctttttct ctttctagaa gtttttgata
     5941 tacttctttt tcgtcaagag cgatccctaa acagaatgtt tgctttgcta aaaacttaaa
     6001 tgcttcttct tgactatcca atggctgttg taaaaaaatg ttctcttcat gaatgattgt
     6061 catgataagt ctcctgctct tcctttttcg ataactgtac gatttgctgc tttattatcc
     6121 atcttcttgt ctttatcagt tactcttttc ttttggatta atcctagtat cactccacca
     6181 acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac tacaggtaac
     6241 actaaaaaac caccatgtgg tgctggaact tggacgccaa acaaataagt taagacagca
     6301 gcaattgatg agcctaacat catcgtgggt aacactcgga tcgggtcttt cgcagcaaac
     6361 ggaatagctc cttccgtaat atgagttgat cctaaaataa aattaaccaa acctgcatta
     6421 cgatcattgg tatcaaaata tttaccaaac aataaagtag caaaaccagt aatcaacggt
     6481 ggtgcaatac aagctgctga cacaccagcc ataaaagtcg tgtttccttg tgccaaaaga
     6541 gctgttcctg ttacatatgc cgccttatta accggtcctc ccatatcaaa cgcacacata
     6601 cagccaacaa tcacgccaag aacaagtgga ttcgaatgtt caaatccagc taaaaagctc
     6661 atcattcctt catttatagc cttcattgga cctgccagca aagacatcac gatgccaata
     6721 aatgctacac caataactgg atataagaag attgccttca agccatctag ttgtttaggt
     6781 aaaattgtca gtagcttttg caaaaataag ataaaataac ctgctaagaa cccagctaaa
     6841 atacctccta aaaatccagt accgccattc atggctacga ttccaccaac aaatccaacg
     6901 actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac tggaaccatc
     6961 attcccattg ctgcatcgcc aactgctttt accattgccg ctgtagcatt gtattgtgca
     7021 tgatctggat caaaagaata gattccccaa acggcaaatg aaatagcaat caatactccg
     7081 ccagctacca cgaaaggcaa catatgagag acaccgttca ttaaattttt atagatgcta
     7141 cgaccaaaat tagcaccact catcgcttct ggttctaaag tatcagcagc ttcattatct
     7201 gctaagacag taccttttcc tgccaatgct tcgtcaatca gccgttgtgc atccttgatt
     7261 cctgtactga cagaaacatc aatgactcgt tttcccgcaa atctttctga atggacatct
     7321 ttatctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt taattcattt
     7381 tcgatcccaa tttgtccatg ggtctccact ttgatcttga tgcctttttt cctagctgct
     7441 tgttctaatg cctcttgcgc catgtaagta tgcgcaattc ctgtcggaca accggttgct
     7501 gcaattagtt gatattttcc catgtttcaa acctctcttc cttgattttg atttgtttac
     7561 ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcggtt gagctgcctg
     7621 ctgctatgct tttacgtaac gtttcatcaa gcggtcgtcg attcatatat cctgctaaaa
     7681 aagcgcctaa catggtatca cctgcacatg ctgtattgac aacttttcct tttggcgagt
     7741 ttcctcgaaa caccctgtct ttcgtaaaaa gaatcgctcc tttctcaccg agggataaca
     7801 aaacattttc tgcacctaac tctactagtc gttgtccata aacaaagtaa tcttcatctg
     7861 taaccatttt tcgtccgaac caagaagaaa gttcttcctc gttcggtttc agcaaaaacg
     7921 gtcgataggg taaacaatcc atggcttctt gatcactact atcaataatc aaatgaatcc
     7981 ccttttcctg acaaatccga ctgatttcta ttaaaacctt tggagacagc cctcttggta
     8041 aactcccaga aacacaaaga taatcttctg cttgtaatgc tcttatttga tttaaaaaat
     8101 tatggacagc tgtctgcggt attttaggtc cttgattcac taatttgtat tcttctcccg
     8161 tttgattgac ttgtgtaaat acattgatac gcgtcatacc aggcacttca ataaactctg
     8221 tttggatttg tttctgctgt aaatagtctt ctatatactt tcctgtaaac ccagcagaaa
     8281 aacctaatgc cgtacttggg aaatctagca tttttaatac tagtgacaca ttcacccctt
     8341 ttccgtttgc ctgaatgtca tcctcttttg tgcggtttac cataaaagga tgcatctcct
     8401 ctgtttcaat aaatagatca attgccaaat tcatcgtgca tgtgtagata gacataacca
     8461 acgacctcct tgactctatt atgaaatacg cgaaagagaa aatgttttct taatcgtccc
     8521 aacacaaaga aaacctttac atttataaac tcgtcgtgct attttttaac taacttttgg
     8581 tgaaagaagg aaagaatatg gatcacaaat taagtgaaac agaacagtac ctctggaact
     8641 ttatcgaaca ccacatccta gagatcccta actactccat cgtgaaacta agtgaacaag
     8701 caaatgtctc aacagccacc attgtgcgga caatgaaaaa gaaaggctat gaaggattta
     8761 cttcgtttaa gcaccatcta aaagaaggag aaaacaagaa catcaatttt tctttcttag
     8821 ataaagtaga caaaggtatc cgtcgagcca ttctaaaaaa tgaacaagaa gtggtacgta
     8881 cgatcaatat gttggaaatc ggaaatattg aggatgcaat acaaagaatc aaatttgctg
     8941 accgggtttt tgtctttgca cgaggatttt cagaaatgat tggtcaagag atgctcgtaa
     9001 aacttcaatt aacagggaaa aattgtcaaa tgcatactga tccagaaatc atcaaaagca
     9061 tcagtcggaa actaactaag aaagacagcg ttatttttgt ttctttgaac ggcgagacaa
     9121 aagaactcgt caccgccgca aagaattgct atgatgcaga gattggttcg attctagtta
     9181 cagccaatca ttcctctaca ctgaaagact attgcgagat caatctcgta ggtttcaaat
     9241 cagaaggttc ttatttccct gattatgaag tacggtcacg tttgcctctc cagattatag
     9301 cgagaatcct gcttgacgcc tatgtattgc gtatggggga gaattgaaaa gcagtttatt
     9361 atggttggtt acagtttaaa acatcacatt ttttattctt tatattgtat ggactcacgg
     9421 aaacttttta tgtataaagt tattcacaat ctataaagtt actttaaata acaaaaaaat
     9481 aattgaaaat acttaaaatc aaaaaatatc tttttaatca ataaaaaaat tgacagtttt
     9541 attaataata agctattctg ttatagaaga aaaaattgtt tgttattatt agcaacaaaa
     9601 aaaggagtgc atcatgaaaa agttatctca aaaacgttta gctcaaatca ttattgattc
     9661 tagaaaatct ctgaaaatga cacaacaaca acttgcagta gctactggta tcaaccgtgc
     9721 cttaattagt cgattagaat ctctcgattt tatcccgtca atacaacaat tagaatcatt
     9781 agcagaagtt ctaaattttg aaccaacaga tctatttata aatgaatcaa ataaatcaaa
     9841 cagaacaaaa catcagtcga agaatattgc tgtagccggt actggttacg taggtctttc
     9901 cattgctact cttcttgccc agcacaatca ggtaacagca gtagacattc ttcctgaaaa
     9961 agtaaaacaa attaacaata agaaatctcc aatacaagac gattacattg aaaaatatct
    10021 agctgaaaaa aatcttaatt taattgcaac gactgatggt gcagccgcct atagagaagc
    10081 cgattttgtt gtcattgctg ctccaacaaa ttatgatagt aaacaaaatt attttgatac
    10141 ttctgctgta gagtcagtaa tcaaattggt gttagaagtc aatccaaagg caataatggt
    10201 catcaagtcg acggtacccg taggctatac cgaaagtatt cgtaaaaaat acaatactaa
    10261 aaatattcta ttcagcccag aatttctcag agaatctaag gcattatatg acaatctata
    10321 tccttcgcga attattgtcg gcacggacca aactgacaaa actttacgag aagcctctca
    10381 cacttttgca gatttactcc aagaaggagc aatcaaaaaa gatatcgaaa ctcttttcat
    10441 gggattcaca gaagctgaag cagttaaatt attcgctaat acttaccttg cacttcgagt
    10501 atcttacttc aatgagctgg atacttatgc agaaatgaaa ggactaaata cacaagaaat
    10561 cattcacggg gtttgcttag atccaagaat tggcacacat tataataatc catcttttgg
    10621 atatggaggc tattgtttac cgaaagatac aaaacaactt ctcactaact ataacgatgt
    10681 tcctcagaat atgataagtg caattgtcga tagtaatcgt acaagaaaag actttatagc
    10741 agatcgagtg ttagaacttg ctggcacgta tgaggctaac agttcgtgga atgcagaaaa
    10801 agaaaaagag gtagtcatag gtgtataccg tcttacaatg aagagtaatt cagacaactt
    10861 ccgtcagtca tctattcaag gagttatgaa gcgaattaaa gcaaaggggg caactattgt
    10921 aatttatgaa ccaacacttg aaaataacac tacatttttt ggttcaaaag taataaataa
    10981 tttagatgat tttaaaaata tcagtcacag tattattgct aatcgttacg atagtaa
//
LOCUS       DACCXW010000097        11002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCXW010000097 DACCXW010000000
VERSION     DACCXW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.098"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            73..888
                     /gene="thiD"
                     /locus_tag="IVY71_002143"
     CDS             73..888
                     /gene="thiD"
                     /locus_tag="IVY71_002143"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7986183.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1217..2668)
                     /gene="cls"
                     /locus_tag="IVY71_002144"
     CDS             complement(1217..2668)
                     /gene="cls"
                     /locus_tag="IVY71_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7986184.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3009..5675
                     /locus_tag="IVY71_002145"
     CDS             3009..5675
                     /locus_tag="IVY71_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7986185.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6039..6791
                     /locus_tag="IVY71_002146"
     CDS             6039..6791
                     /locus_tag="IVY71_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7986186.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6813..7967)
                     /locus_tag="IVY71_002147"
     CDS             complement(6813..7967)
                     /locus_tag="IVY71_002147"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7986187.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8152..10593
                     /locus_tag="IVY71_002148"
     CDS             8152..10593
                     /locus_tag="IVY71_002148"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7986188.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(10750..>11002)
                     /locus_tag="IVY71_002149"
     CDS             complement(10750..>11002)
                     /locus_tag="IVY71_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7986189.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         3565 a         1966 c         2368 g         3103 t
ORIGIN      
        1 ttgaaaaaca tatgatagaa acagtaacag tattatgaaa aatcttttta taaaaatgga
       61 ggaatcgatt tgatggaaga tcagtatgta caagcaatga cgatcgcagg atcagatagc
      121 gatggaagcg cagggatgca agcagatatg catacattct ttacaagaaa tgtttacggt
      181 gtttctgtca tgaccgcatg tgttgcaggt aattcttacg gaatcggagc aagtgtgaca
      241 ttgccaacag attttatcga caaagaattt gaactgattg cgaaggactt tcaaatacgt
      301 gcagctaaaa caggtatgct agcagattca aagttgattg agacagttgt aaaaaattac
      361 caaaaatatg attttggacc attagtcgtg gatcccgtaa tcgtaacgaa acatggaaat
      421 cttttactag aagaatctgc tttgcaaagc ttgaaagaaa agcttgtccc tttggccgaa
      481 gtactgacac ctaattttta tgaagcagaa aagctgactg gacggacatt agcagctgaa
      541 gaagattttg tgcaagcagc aaaagaactg caagatatgg gtgcaaaaaa cgttatgatc
      601 aaaggacatc atgctaaaaa tgcgcagcaa gaaacagtca atgactacgt attgttcgaa
      661 tcaggaaaat cattttggtt aaatgcacca ttttatccta ccgaccggat caacggaact
      721 ggcgattcgc tatctgcatg tattgcagct gagttagcga aaggaaaaac agttgaacaa
      781 gcgatccgat tggcaaaaga ctatgtaaac cgtgcaattc gtgaagaaat aaatgtaggg
      841 cacaaatatg gccccatcaa tcattgggca gctggtgaat tagagtaaaa gaaagaagtt
      901 gtgaaaaagt tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
      961 ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
     1021 ggtcgtgagc ctgccgtcca gctccgagtc acaaggaaca agttcaaaaa acagcttctc
     1081 atgtttgttg aatattgtga cttgtgaccg aggagttggc aggagaacac cgtttgttcg
     1141 gaagaaggga ctgtgacaag atttttgtca cagtcccttt aatcttagta tatttttctc
     1201 tacttgaata aaaaaattat aggattggag aaaataatcg agaaatttgt tgcttgaata
     1261 atagccagtt agacatttcc ttgactgttt cagttgtcat ggatgaacaa tgtgtctcat
     1321 cttctttgaa ctgagtagca agttcttcta aaaactcagg atcgtaaatg acagcacttg
     1381 tttcaaaatt caatcggtag ctacgaatat cttgatttgc tgaacctacc atgcagattt
     1441 catcatccat tatcaatgtt tttgcatgga ggaagccgtt ctcatagaca aggatttgta
     1501 cattttcctt gatcagctgc cgagcataat attgtgttgc tcgataaata aaaggatgat
     1561 ccggtttgtt gggaatcatg attttcacat ctacaccaga ggcagttgcg atttttaaag
     1621 cagcaatgac actttcatca ggaacaaggt aaggtgtctg tatccaaaca cgttttttag
     1681 cagaagtaat caatttgata aatgaaagct tgatttgttc cctttcgtta ttcggtccgc
     1741 ttgaaacaag ctggatggat gtatcagcaa gcttagattc atctcgatct gcttttttaa
     1801 agaaataatt caaatgatag ccgacctttt tttcttcagg taccgagacg ttccagtctg
     1861 ttaagaagcg aagctggagc aaagaagaag cggcaccgaa aatccgtata tgcgtatcgc
     1921 gccaatagcc gaattttttt gttgtattta catattgatt ggcaatattg aaaccaccgg
     1981 tataactaat ctttccatcg atcacaacga tttttcggtg atcatggtaa ttcaatcgga
     2041 aacgaagtaa tgccctttgt gaggtaacaa atgtatggac atgtccacca tttttgatta
     2101 gacgattgaa atcttttgct tttgtgccat gagagccaaa tgcatcatat aatattcgaa
     2161 cttccacgcc ttctgcggct ttttcttcta aaacatgtaa gacttgctgg ccgatattat
     2221 ctgtcacaaa tgcgtaatat tcgatatgga tcgaatgttt ggcttttttt agatcttgaa
     2281 gcagtgaatc caatttctct tgtccgtctg tgagaagagt gacagaattc attcttgtca
     2341 gcggcatacg atttaatgaa gagaagaagt caacaaattg ctgtttgtct ttatcgccca
     2401 tgtcaggatc gtaatgttca atggtatctc ctctaaaaga ctgaaagttt tctaattctt
     2461 tcaagtcact ttgtcggaga tagaattttt tcttatccgt taatccacgt ccgaaaaata
     2521 aatataatac aaagcccacc ccaggaaggg caaacaatac taagagccat gcccaaatgg
     2581 ctgctacatc tcggggtttg atcaaaatag ccaccaaagc aataagtgca tttaatagat
     2641 aaagggcggt tataatacta gataccacaa aaatgttcct ccttacatac aataacatta
     2701 gtatagcatg atagaagaaa gaagcctacc aaggtgaata tacttttcac tcctttcatg
     2761 aaaatgtagg taaatcgggc taaagtagtt gctttttgta ggttaataag gtaggatatt
     2821 agtacagttg aatatctcct taggggagta accggcaacg tttgttgcga taatatcaac
     2881 agtaagttaa ccaaatggtt ttctggtatt gtatgaaatg gtgagaccta agacaaatgc
     2941 gtatttgtct taggtctctt ttttttcaga caggactagg gccaagatca aaaaatgggg
     3001 gatgaaattt ggaaaacaag gaacaagcac aacaagcggt ctataaagaa acgatcgact
     3061 cgttgatgca gcaaacgcag agctcaaccg atggattgag caccacgcaa gcgaaggaaa
     3121 aattagaaag agatggcttg aaccagctaa aagaaaagcc gaagaagcca acttggaaat
     3181 tgttcttaga aactttcaaa gatgcaatgg ttatcgtatt gctgattgtc gcactgattc
     3241 agatggtcat gggtgactac atcgaattct tagttatttt tgcagtattg atgttgaatt
     3301 ctataatcag tgttgtccaa acaaaaaaag cagaaggttc tttagaagcg ttgaaaaacc
     3361 tttcagcgcc cgatgctcga gtgattcgtg atggtaagga aaaaacgatt ccggcaaacg
     3421 aacttgtggt aggagatatc gtgttgttag acgcaggcga ttatgtacct gctgatggac
     3481 gtttactaga tgcgggatca ttaaaagtag atgaaggtat gctgacagga gagtcgactt
     3541 ctgcagaaaa agaagtaacg gatatcacaa gtacggtacc aatcggagat cgtacaaata
     3601 tggtattcag tggaacgatc gtaacttacg gacgcggtaa attcttagtg acttcaactg
     3661 ggaataatac ggagatcgga caagttgctg gccttttgga atcaacaact gagcaagtca
     3721 ctccgttaca acgaggtctg gataaattca gtaaaaaact aagtttggct attttggcat
     3781 tgtctttatt gattttaggt atccaactgt ttcgtattta tcttggagaa ggtacaggag
     3841 atatgactgc agatatcgtc agcgcagtga tgtttgccgt agccgtagcc gtagctgcta
     3901 ttccagaagc attacagtct atcgtaacaa tcgtgttatc tttgggaacg aataaaatgg
     3961 caaaacgtca tgccattatt cggaagcttc cagcagttga aacacttggt tcgacaagta
     4021 tcatctgtac agataagact ggtacattga cacaaaacaa aatgacagtt gttgactact
     4081 ttttaggtaa tggcgatact ggggatttca caaatgaccc ttcaaaatgg acagctgacg
     4141 aacgtcgctt gatcgaaatc agtgttttag cgaatgatgc atcgatcagt gaagacggta
     4201 ctaaattggg cgaccctacg gaagtagcgt ttatcgactt tagtgaaaaa ttaaaccagc
     4261 cttatcagga aatccgaaac aactacccaa gacaagcaga acttcctttt gactcagatc
     4321 gaaaattaat gtctactgga cacacaattg ataacaaaaa ttacttgttt gttaaaggcg
     4381 gaccagatat cgtcttccaa cgcagtaaaa aagtattgct aaatggtgaa gtcgttccga
     4441 tgacggatga attgatgaaa aaattccaag agcaaaatga agctttttca acacgcgcat
     4501 tacgtgtact agcttttgct taccgtccac ttgataattt tgatttatca tttgaagatg
     4561 aagacgattt gatccttgtt ggtttgcttg ctatgatcga tcctccacgt gctgaagtga
     4621 ctcaggcggt aacagaagca agaagtgcag gaatcaaaac aatcatgatt actggtgacc
     4681 acaaaacaac agctgttgct atcgcaaaac aaatcgggat ttttgaagaa ggaaacctgg
     4741 ctttgaccgg gacggaacta gatgaattga cagaagatca actaaacgaa gttttagaaa
     4801 aagtcactgt ttatgcacgt gtttcacctg agaataaaat tcgtatcgtt cgtgcttggc
     4861 aaaataaagg tcatatctca gctatgacag gtgatggtgt aaatgacgca ccagcattga
     4921 aacaagcaga catcggaatt gctatgggaa cgggtacaga tgtggcaaaa gatgcagcag
     4981 cgatggtatt gacggatgat aactttgctt ctattgtcag cgcagtagaa gtaggacgta
     5041 atgtgtttga taatatcaag aaagctattt cttatctatt tgcaggaaat ttaggtgcga
     5101 tcattgcgat catctttgcc ttagttgctg actggtccaa tccatttaca gcattacaac
     5161 tgttgttcat caatttagtc aatgattcac tgccagcaat tgcattaggt acagaaaaag
     5221 ctgaacctgc tacgatgaac cgcccgccaa gagatccaaa ttcaggaatc tttacggaca
     5281 aaacattgat ttctgtaacg tatcgtggtg tgctgattgg gattgctgtt atctttgctc
     5341 aatggcttgg catgcagcat tcacctgaat tcggtgtagc catgtcattt tcaacattga
     5401 tttggtgtcg gacattgcaa acattcccag cacgttcaaa cacacaaaca gctatccaag
     5461 cagggttctt ctctaacaga agtgtcttac tagctgtagt tgtatgtagc gcgctttact
     5521 gcttaacaat gatcccagga ctgaaagaag tcttctctat cccatcagca tttggattga
     5581 cagaagtctt tacatgtatg ggaatcgcac ttgtgacaat tgttttgatg gaaatcacta
     5641 aattgatcat tgtacgagta caaggaaata aataaaacga atcaacgagg ttgtgagcct
     5701 gccgtttaat tctgagtcac aaggtgcaat ttcaacaaac agcttctcat gtttgttgaa
     5761 tattgtgact tgtgaccgaa gagttggcag gagaacaccg tttaaggaag aagttgtaaa
     5821 atcagcattt tgagctaata ctgagggtca tctgattgga cactgtttat tcggaaagag
     5881 ggactttgac acgactttgg tcacagtccc tttttttatt tatgatagac ggaaagccaa
     5941 tcattttatt attcatcaaa agagatagaa tagataaaag aggagaatga tttattccga
     6001 aaagtgaaaa catataacaa aaaattagag gaaatcaaat gaatgaaaaa gaatgggatg
     6061 aatttgctga agaatattat gaaatccagc aagaatcgca agtaactatt gctgaagatg
     6121 taaaaaatta tctgaaaacg gagaaagtcc tgcctgctga aacagcagca gacgttgctg
     6181 gaggatctgg cagatttctt tccttagcaa aagaaattaa tcgctatgat ttgatcgatt
     6241 tttcttcagg aatgctagaa aatgcaaaaa aagaagcaga aaaaaatgag ttgccaaatg
     6301 tttcctttat caaacagtcg ttagaagaat ttatccaatc aaatgagaca tatgaaatgg
     6361 tttttacagc agcaaatcca gcattggtac atcttattca attagaaaaa ttgatgaaaa
     6421 agtcaagcaa atggtgcgtg ataatcagag tagtggaatc aatcgatgat gttttttatc
     6481 ctatcgaaaa gcaactagga atgatagaag aagatcctaa tacatcgcca gttgtaatgg
     6541 atcaatttga aacatactta attgatgaag gattcaaagt gaagaaaaaa gattttactt
     6601 attttacaca ggaatcaatt actgaagaat tgatgaagct atactatgac gcgtacgcaa
     6661 aagatcctgt tgtgaccagg tacattcgca aactattcag tgaaaaacga gaaatcctta
     6721 gtacgactaa gctgacctat agactgctcc tttggtccgc agataaaaaa agaacctttc
     6781 acgactcgtg aagggttctt tttatgattt tattatattt tgtagtaacg aatatcattt
     6841 tcgatctttg taatcgaaaa aggtacatct gaagtatatt ctgcacattc tccgtgttca
     6901 ggaagcgtct cactgaaaat cgtttcatat tcgtcaacag tgatccgtgt tcgagaatct
     6961 agcatctctt gatgagattg tgcgaagaga tattcttgat agttttcttc cagataacct
     7021 gtaaaaaatt cacttactgc accagaaccg tagctgaata gaccgatgcg atctcctggc
     7081 tgtagtgatt tcgaattttc cagtagagaa gttagcccca ggtataatga accagtgtaa
     7141 agattaccga ttcgtcggct gtaacggatg ctttcttcat agcgagccat aagacgttcc
     7201 tgattttctt cgtctgtttg gtctaatacg ctttgcaatg ccttttttcc catcttagta
     7261 tacggaatat ggaaagcaat cgcctgataa tcttcgagtc ctcgacccga caattcttta
     7321 tgtcgattcc aaactttttg gaatgattcg atatacgtag aattagataa aggaccatca
     7381 acaacaggaa attcgctata atctggacgc cagaaatcat agatatcttc tgtcagaaat
     7441 acgctgtcgt cttcaatcga taaaatacgc gggttttgag tgatcatcat cgcaacagca
     7501 ccgacacctt gcgtcacttc accaccgctt gccaagctgt aacgagcaat atcacttgct
     7561 atgactagta cttttcgttc tggatgattt ttgacatatt ctttcgccat gtgcaaagct
     7621 gcggtgccac cgtagcaagc ctcttttact tcaaaagaac gagcaaaaga ttggattttc
     7681 agtaaatggt ggataattac cgcactcgct tttgaatgat caatcccgga ttctgttccg
     7741 acgatgacca tgtcgattag ctcacggtct ttttctgtta cgattttgct ggcagcattt
     7801 gctcctagtg taatgatgtc ttcatttgca cgattgactg ccatttggtc ttgaccaatc
     7861 cctatatggt attttgcagg atcatcccca cggctttctg ccaactctgt catatctaaa
     7921 tataaattag gaataaaaaa ggaaagacga tcaatcccta ttttcatttc aacacctctt
     7981 tacttatgat aaaaaactta ctttactaag gtatcataaa aagattaaaa gaaatataaa
     8041 aaagagaata tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag
     8101 ctatttgttt ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa
     8161 gtcgttatga tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca
     8221 tttactgcag ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg
     8281 aaaaaagata aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa
     8341 aatgttgcca gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg
     8401 atcaatgaag tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa
     8461 ttaggtgaag cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg
     8521 ctgaaacagt atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac
     8581 gacggattga ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca
     8641 acacaatttt ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa
     8701 gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct
     8761 gatgaagacg tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg
     8821 ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg
     8881 ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat
     8941 ctgccttatt tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg
     9001 ggaatcgctc caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca
     9061 acaatcgatc tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa
     9121 gaactgcagt tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat
     9181 ccaattggtg caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa
     9241 cagaagagat acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg
     9301 ttagaagcga atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt
     9361 acgccatctg aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg
     9421 ttgatttttc aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa
     9481 gtgagtgaag tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa
     9541 aaatggattc cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca
     9601 aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt
     9661 ctatcaggaa aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag
     9721 ctgattcttt gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa
     9781 gatatctcta ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta
     9841 gtggatgtaa aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca
     9901 aacaagttac gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt
     9961 gctacagaat cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac
    10021 aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt
    10081 gacccttacc gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg
    10141 gcagctacag gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga
    10201 aatggattat accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa
    10261 ctaacggtgc ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca
    10321 aaagcatctt tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct
    10381 gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa
    10441 aaaggccaca tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag
    10501 gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct
    10561 atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag
    10621 caagatttac agttatttta tttactgcag atcttgcttt tttggctctt cgtcaaattg
    10681 tgttggcgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt ctgtgcgccg
    10741 catatttttt tagttttcaa agatcagacc ttgttgtaaa tagatccgtg cccgaaagtt
    10801 ataaaagttg cgataaccaa acgcaattcg tttaatcagc ttgatcttgt tattcagccc
    10861 ctcagtgacg ccgtttgagt ggggcacctt aaaggcattt gaaatacccg attggtagcg
    10921 atggaagatc gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa ttgttttaag
    10981 ggtttcttga aagccttccg ta
//
LOCUS       DACCXW010000098        10961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCXW010000098 DACCXW010000000
VERSION     DACCXW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.099"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            92..1051
                     /locus_tag="IVY71_002150"
     CDS             92..1051
                     /locus_tag="IVY71_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7986190.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1316..1795
                     /locus_tag="IVY71_002151"
     CDS             1316..1795
                     /locus_tag="IVY71_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986191.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            1828..4056
                     /locus_tag="IVY71_002152"
     CDS             1828..4056
                     /locus_tag="IVY71_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7986192.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            4185..5828
                     /locus_tag="IVY71_002153"
     CDS             4185..5828
                     /locus_tag="IVY71_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7986193.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(5901..6446)
                     /locus_tag="IVY71_002154"
     CDS             complement(5901..6446)
                     /locus_tag="IVY71_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7986194.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(6469..6954)
                     /locus_tag="IVY71_002155"
     CDS             complement(6469..6954)
                     /locus_tag="IVY71_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7986195.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(6944..7756)
                     /locus_tag="IVY71_002156"
     CDS             complement(6944..7756)
                     /locus_tag="IVY71_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7986196.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(7895..9427)
                     /locus_tag="IVY71_002157"
     CDS             complement(7895..9427)
                     /locus_tag="IVY71_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7986197.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(9536..10567)
                     /locus_tag="IVY71_002158"
     CDS             complement(9536..10567)
                     /locus_tag="IVY71_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7986198.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3670 a         2018 c         2100 g         3173 t
ORIGIN      
        1 ccgattgtaa aattaagcta gacaactaaa aaagtcattt gtgctacact caaaatagtt
       61 ccgaccaaag aattataagg agtgagtaca aatgacctat acccatctta caacggatga
      121 acttgtaata atagagtctt atttcaaaat gaatcaatct gttgctaaaa ctgcccattg
      181 cttgaatcgt tcaagacaaa cgatccataa agtatatctg ttcttcaagc aaggaaaatc
      241 agccttagaa tattatcaac agtataagaa aaacaaatca aactgtggta gacgtccgct
      301 tgttttaccc gaggaacaat cagaatatat tcaaagaaag gttgttcaag gatggacacc
      361 cgatgtgatt gttggtcgtg cagcgtttcc tattagttgt tctgctcgta ccatttatcg
      421 tatgttcaaa aaggggctat ttgattcttc tgacttaccg atgaaaggca agcgtaaacc
      481 gaatggacat caagaaagac gtggaaaaca aactttccgc cgctctattc atgaacgtga
      541 aaaggattat agccaattct caaatgagtt tggtcacctt gaaggtgaca ctatcgtagg
      601 tctgaaacat aaaagtgctg taattacctt agttgaacga ttatcaaaag ttatcatcac
      661 attgaaaccg tgtggtagac aagcgattga tattgaaaaa aaattaaatc aatggtttga
      721 atctgtaccg aaaaacctat tcaaatccat cacttttgat tgtggaaagg aattttcaaa
      781 ttggaaacag atcagtaatg tcaatgatat tgccatttat ttcgctgatc caggaacgcc
      841 gtctcaaaga ggcctaaacg agaattctaa cggattgtta cgtagagatg gtttattgaa
      901 atctatggat ttcaattcag tagatgaatt ttttattcaa tctgtcgcat ctaaacgaaa
      961 taatattcct agaaaatcac tggattatcg aacacctttg gaagtatttt tgagttacgt
     1021 aagtattgat gatctgtcta acttaatttg acaattaaga atacaaaaaa gatgttggtg
     1081 aagttcacca acatcaaaaa gtatagagcc aaaatattcc aacatcatct tggactttct
     1141 acatgttact aatgagcgtg taaaagaacg agatggtgtt tttatttcta tcaatgaagc
     1201 tctttttctt tgcaatcctc acttcaatcc tctataatgt cgcatatagt ttagtaaatg
     1261 agtaaactac taatttactt atttactaaa aataaaagga tgaggagtta aaataatgga
     1321 aataccaaca aatttaaaac ataaaccagt aatcgttgta gaagactatg ataaagttga
     1381 tggaagaaat gcattaaata tggatgcaaa agggctttca ctgggccttg ctcagtggaa
     1441 tgatcgtgga aaagtcgata tctcaggcaa ggtatggcga catactggag aaaaatggtc
     1501 acgacagtcg gaagaattac caatcacacg tattttagat ttagcgatct tagctgcgca
     1561 aggaagttta tacttccaag atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc
     1621 agtcgtagac atcattggat tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa
     1681 tccaaagatc gatgaagaca tcattttgta ttacgataca ctacaaaaag acggagaact
     1741 gattggggaa cgattccgta ttttaaaacg tcttttagat gagctaggat attaatagac
     1801 agagatgatc aaaaggggaa gagaagcatg gagaatttct cactaacaga aaaccgaacc
     1861 ttttcacggc tagaaaagca aatgatctgg aagaaaccga aaagccatcg gacaagtgca
     1921 gaagaatggc ggatcgcctc cgaaatcaaa gggaattggc aagatcctga aatgaaaatc
     1981 agtaacgtac ttttggaagg agatgccggg tcggggaaga cacaattagc caaagcacta
     2041 tctgctgatc tgcaacttcc ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca
     2101 gatgttttcg gagccttatt acctgtagta gattctaata aggaacaaga ccaagaatta
     2161 ctagaagcta tttaccaaac agatacactt gaagctgtac tgacactgat ccagaaccat
     2221 tacggcattg atcctttttc tgctaaagaa aaattagcag agctagttca aaggatcgaa
     2281 gctgattatc cagatgcaat ccattacagc tattaccctt ctgaaatcgt tcgagcgatt
     2341 gaaaaaggct atttattaga gattcaagag ccgactgtca tccgagatgc ctctgttctt
     2401 gtggcactta actcagcgct cgaaccaaat gggatgttga atttaccgac aggaatcatt
     2461 cgtcgccatc cagattgcgt cattgtgatc actacaaatc gaaattacca aggaaatcgc
     2521 ccattaaatg aatctttaag agatcggatg caacatgcgg aaaaaatgga tcttcctgcc
     2581 ttagaagtca tggcagaacg tgcgatggcg aaaactatga tccaaaaaca agaacttctc
     2641 ttaaagatgg cagagattat ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa
     2701 ggtgtagcag gaatgcgttc ttatttttat tgggcaaata cctataagca aggacaagat
     2761 ttactccagt cgatttatcc aaaagttttg tataaattat ccactgattc agatgaattg
     2821 gcaattcttg aacaagcttt gtctgacagt ggattgctac tggaacttca ggacatattg
     2881 agaaaagaaa aatggggtca tcctcaatct gagcatacaa aagaaagaac cattagcgcc
     2941 gaagaagcaa atgaacgaaa catcactgaa aatgctgaag aaattctgcg cttagatgaa
     3001 aagaaatcgt tgtctgctga agaaccaaat gagggagaag agaaacaaga agaaacaata
     3061 gaaaaaaacg agattcctcc tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa
     3121 aatcaaaaag aacaacaaac aggagatgat tctcaagaaa tgtctgcttc tgacatggaa
     3181 gcacatgata aggaaatcaa gaagcaactg aataaagaag cgcgacaaat catgaaggaa
     3241 acgattcatg aaaaagaagg tctcatcgtt caccgaccag ctttccaagg aaaaacgccg
     3301 gaagcacgtg agctacacat gcaagtaatg ccaatagtag aatcacttag ccggcaaatt
     3361 ttagagttac tggataatga acaaacagag acgtaccaaa aaggaaaata cgaaggtcag
     3421 cggttcaatg caagcaaagt agcatatggt gatttgagaa attttgacaa aaagaacccg
     3481 ccacatgagc aaccttcttt agcggtagct gtacggatcg atgaatctgg gtcaatgata
     3541 cgggatgatc ggatcgaagc ggcaaaaaaa gcagcgattg ccattgccga attttctaag
     3601 aaagtcgata tcccattgtt gatttacgga gatacagcag accgttcgtc aagagaaaag
     3661 acttctttgt tttcttataa agaatttgaa gacggatttc aatggttaaa tgaaaaatta
     3721 gtcactatga aaccaaggca aaacaatcgt gatggtgctg cacttcgttt agcagcagaa
     3781 aaattaaaca gacaatccgc aacaaccaaa ctgttgctta atattagtga cggtcagcca
     3841 aaagcgttgc cggattatac aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa
     3901 gaatatgaac gtcaaggaat cctatttgtc tccgcagcta ttggccaaga caaagaagaa
     3961 atcaagtcga tctacggtga aagtcgtttt gtagatatta ctgacttgaa tgaatttccc
     4021 aagcaaatga ttcaactgat tgcccgctat ctatgataaa ataaagctca acgaacgcac
     4081 ttagcagatt cgttgagctt ttcgtttcta gttaccggta acaaggatac ttctaaaata
     4141 gaatcggtta taatcaagag ggaccgttaa aggagtgaaa cgatatgata caagaacaaa
     4201 aatggtggaa agaagcagta ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg
     4261 gcgatggtca aggagacatc aatgggatcc gtgaaaaact taattatttg aaagatcttg
     4321 gtatcggctt tatctggatc aatccgatct atcaatctcc atttgtcgat aatggttatg
     4381 acgtaagtga ctatcaagat atagaagaga gattcggaac gatggaagat tttgatttat
     4441 tgctaaaaga agcacatgaa ttagggatca aagtgattat ggatcttgtc atcaaccata
     4501 gttcggatca gcatcaatgg tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg
     4561 attattacat ttgggtcgac ggggtagatg gcaaagaacc gaataattgg acatcgattt
     4621 tcggcggttc tgcttgggag tattctcatg aaactggaca gtattatctg catgtttttg
     4681 caaaagaaca accagactta aattgggaaa gcgagaaatt gaaagaagaa ctattcaata
     4741 tgatccgatg gtggttggat aaagggatag atggatttcg tttagatgcg atttctcatg
     4801 taaaaaaaga tgaatattca gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg
     4861 tctcaggaat cgaagaacat ttgaccgatt tgaaacatgt ttttgaagag tacgatatca
     4921 tgactgtcgg agaagcaagt ggtgtcactg cagaagaagg accacaatgg gtagggaaag
     4981 acggttattt cgatatgata ttcgaatttg atcatatcca tatctggcag caagaaaaag
     5041 aagggcaatt agatgtcctt aaattgaaac atgcattaag cgcttggcaa acttcattgg
     5101 atggtatcgg atggaacgct ttatatatgg aaaaccatga tgttcctcgt gccgtttcag
     5161 tgtttggaga tactcgtccc gatttttggg ctatgtctgc aaaagcaatc gctatgatgt
     5221 atttcttttt gcaaggtacg ccatttattt atcaaggaca ggaaatcgga atgacgaata
     5281 tgccctttga atcgatcgat caagttgatg cagtagattc gaaaagactt tataaaaggc
     5341 tgcttgcgga aggtaagaca agagaagaag cattggatat cattcgtgaa acaacgagag
     5401 acaacagcag gacacccatg caatggactt cggaacaata tgctggtttt tctacacatg
     5461 aaccttggct gatcactaat ccaaatacca aaacgatcaa tgtagaacaa caagaatatg
     5521 aaccagagtc ggtcctgcaa ttctataaga acatgatccg cattagacaa accaataaag
     5581 gtctgatata cggttcttat aaagaatatc ttcatgaaca tccacaactt tatgtctatg
     5641 aaagatattt agaagatgaa caatatctta ttatggtaaa cttaacagaa tcacttgctg
     5701 actatgaact tccaaaagaa gcagatcagt cctggacatt actcttgtca aattcctcta
     5761 gcggagaatt tgaagctaaa ggcatactag caccttatga agctcggtta tataaaacaa
     5821 acaagtaaaa cttcctcgtt aaagttcatt agttggactt tgcgaggaag tttttcagat
     5881 tgctctgtca attactcatt tcaataattc gctctaggac caccgatata tttaggacga
     5941 gagcgaaggg ctgtcacgtg agcttgtcca agggttttca aacgaggtct gacaggtact
     6001 tggacatgct tgatatgcat accgattgat gtgtcaccga tatcgatacc ggcttgtcca
     6061 actacgtgtt ccacttcgac aggatcattg aactggtcaa aagcagcaac agaacaagca
     6121 cctccagcat gtagtgcagg aacaacagag acaatttcga aattttttgc ttctgccact
     6181 gttcgttcta ctactaatgc tcggttcaaa tgttcacaac cttggacagc tagcgcaatt
     6241 tctttaggat ccaacaactc ttttaacgta cggatgatcc attgaccaac ttccgcactg
     6301 gaattttttc caatatgtcc tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga
     6361 tcaccttttt tcaagtgtgc ctcagccaat atgtcattaa ctatttcagt caattggtct
     6421 tttaattctt tttctgacaa cttcattttc taaaaccttc tttttcaatt aatattgctt
     6481 ccgtgcttga atacgttgaa gacttgcagt tataggaaag gcaagtccca ttccaatcag
     6541 attttggaag atgtttcctg gtatagaagc cactccagcc ggccaatcat ataaaatcca
     6601 accggcgata ccatagccga tgatcataac taagcttcct aataaaagcc ctaatcccca
     6661 catacgagta gaagatttgc tgaaagaacc agcaacatat ccttgtaacc cgtggatcac
     6721 taatgaaaaa actgcccact gcggatatcc tgaaatcaaa tcgatcaata gaccactgga
     6781 agcaccaaca actaatccac ccgttggacc ataaagaaaa gcagcggtat agatcccggc
     6841 ctcgcataat gtcacaaatc cattcgtagc cggtacaggg atcaaaattg ttaatgaaag
     6901 ggcaactgtc aacgcaatca gcatagcata gatcgttaat tgcttagttt tcatttctca
     6961 ttcctccttt ttgaataccg ccagaccaga cacttccggt atgatcgttc atcagcacgc
     7021 cattagaaat acaatgggca acaaattttt tactttctgc tactgctttg gtaaaatctg
     7081 cccctaacac aagattcgct gttattgcag aagaaaaggc acaaccggcc ccgtttactg
     7141 ttgtagtgtc gattttttta tgagaaaata cctcataatg atcaattgaa tagagaagat
     7201 ccacagcttc atctccagcc atccttgtgc cgcctttgat cacaatgttt tttgttcctg
     7261 aagcagtgag ttctttagcg agatggatca tttctgttaa cgaagaggga agagcttgac
     7321 cagaaagtaa agcagcttct gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt
     7381 cttcgatgag ctgcgaaata tacaattgat tgatcacagc ttcagtttct ttgaatgcca
     7441 aaacagggtc taaaatgatt gggatatttt gttttgtttt tatgaagtct cgaacaacca
     7501 tgattgcttc acttgaattc aataacccaa ttttgatacc atctaatgaa gtcattttat
     7561 caatcgaaac taactgtcgt ttaagcaatt ccaaagatat agattccagc ataaatcgct
     7621 cattaatatc tagtgcccct atagaagtca aggcgcttag tccgaataga ccaaaattct
     7681 caaaagtttt taaatctgtt tggattcctc caccagcaaa tggatcagaa ccgccaattg
     7741 tcaagattct tttcataaca ttttcctcca tgaataagag tataacgaaa ttttcaaaaa
     7801 agaaaaacag ccaattggca tatttttatc tcatccaatt taaaaaaaca aaaagaaaag
     7861 aaagaccaaa atggatcttt ctttccttaa atagttagga cttttgtgat tttaattctt
     7921 tctcgatttg aagccgttgc actttcattg tagctgttcg cgggattgca tcaaaatcac
     7981 ggatgatagg gacgttcaag cgaggaagtt catggacttg ctcccaccat cgattccaat
     8041 ccatttcttt atcaggagct aaagcaataa tcggttgtgg agaatgattt ttatccctta
     8101 caatcactac ttctgccaaa aaatctaacg aatctaataa aaagtcttca atagctaatg
     8161 tactattgat cgtttcgatc aaatccactt gccgatcttt caaaaatagg tggccatgct
     8221 tatccataaa accgtaatct ccgctatccc accaatcgcc ataaacattt tcctggaacc
     8281 gtgcatcttc cttgtaataa gtcaaagccc gtcctttaga cagtaattgt atatgaccat
     8341 ctgtgttttc aggtaggaca ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt
     8401 ccagtccaac gcccatatct cttgcatcac ttgttttcaa actctctagt gtatgagcct
     8461 taagaatcat tggtccgcat tcactttggc catatacttg aagaaatacc gcatcctgtg
     8521 ccatcgacgt tcgcaaaaat gtcttcattg tctggttgtt tatagcatca aaagtggagt
     8581 gatagtatcg gatacccgaa aaagcttctg gatgctcctt agcagtaaaa cgccactgaa
     8641 caaagttatt cggatgggtc tctagtgcaa ttggccggtg agcttccaac atatgaacaa
     8701 cttttgaact ttgtgcgttt gctaaaggca tcataggaaa tcccatggac atcaaagaag
     8761 agataccaat attgaatctg gaatgcactg gagagatgtg aaaagcgatt aatttctttt
     8821 ccgaaatttt tgtgaaaact gttttttgcc atttcgtccg ccatcccata gaatgagcag
     8881 aatgacaaat aagtttaggg ataccagttg taccagaagt atgggtcata taagaaattt
     8941 catttttgtc taaaaatgat tgagaaacag acaaagcagg ttgttccatt aaataaagaa
     9001 taggaatctg ttttgatttt gttccatttg aaatttctct cactcgcttt tttgtctcat
     9061 catcaaataa aatgaaaggg tcttctaaac gatcgataaa tacttccatt gttgttgttg
     9121 gtagatgata agaagtcatg acaggcactg caccaaggta agacacagaa acagctagaa
     9181 ggtatgtgtc aaaagcggaa ctcttataaa taataacttt gtctccggat ttcactccaa
     9241 gatgagccaa ttgataagcc cgtttcaaca cactttcgca gcattttttg taagttgttt
     9301 caagtcctaa ttctgggaaa gctgaaagtg gctcatcaaa gataatcggg gtctcggaaa
     9361 agttagctgc agcttcttga aaattcgtaa ataagttcaa aggggaatac gttatggtct
     9421 ccatcattca cactccatta tcttttgtta agtaatatac aaattcaaga taatcgtaac
     9481 ataatccttt atataataac atagtttatt gtgaaaaaaa gatcatacat ttgttttata
     9541 tggaagaaac ccgaacgaat gccgctaatt cgtgataaga aaaatcaacc atcttttccc
     9601 agatatcttg aaaagaatgg gatgagaggt atcgattacc acataaataa tttaccgcgt
     9661 cagcatcatc taaatatagt tctgcataat aacgatatgt ttttctgcat agttctggcg
     9721 atacgttttt atatactgta attttgctca aaatttggcg aatagtccga ggagaaattt
     9781 gctgatcatg tgaggaacga aaaacaaaag aattttcgtc cgtattttgt gctgttaact
     9841 taaaatagtt ctccagaaat ggcatcgctt cttttggtat tggtacttta ttcagtctgt
     9901 tttcttttct attcagatta agaatctgct tggtcaaaga tacatcttta cgtttaagtt
     9961 ctactacttc atggatcttt aatccgcaat acagaagaat ggcagtaatt gcaaggttcc
    10021 tcggtctatt cttttcttca aagctagctt ctctgcttgt ttgaaagcct tcaatcggga
    10081 taaacaaatg ctcaaaatct ttgaaaagtt ctgcatcgat atatcgcgga gctgctttgg
    10141 ttgtttccgg taaacttgta cccaaaagag aaatctcttc ttcaatcagt cctttttgat
    10201 acaaaaatcg taaaaaatga tttaatgaat attgcttcct tgaaatcccg taatagctat
    10261 tttcaaacaa ttgctccgtt tcttggttcg ctgtagtttg gaacgaacga gtatgtcgat
    10321 agattttagc taagaaattc tgataatctt gtttttctaa atggataatc agctgatcat
    10381 ccacttcttc agaaaatact tttctcaaat aatcagtaaa cagatgaaaa tcttttgcgt
    10441 attcatagcg actgcctaat gaaagggaag aacgattttc ttctaaaaac tgttgaaaga
    10501 cggaagggag caagtgtgta cgttgcttta acttttcttg cagttgtttt ctagttttgc
    10561 tgtccattac aaattccctc caatagtgtt cattttctat attttaccgc atttcatcaa
    10621 atgaagatag acagtttgtc ttgatcttag gaagcaaatt tttaactaag tactagctaa
    10681 aagatatgaa gtaagaagat acatttttag ttattttatt agtgcagtca ttcttttgat
    10741 cttttaattg atttatcctc tttttagcta tgtggttact tcatgagcaa tgaaatggat
    10801 ttggttcgtt ctaatggcag gaggctcttt cttttatttc cgaaaaatta gaaaacattt
    10861 tttcagaatc gattggctct ccatttattc aataatggtt tgtctaacga tattgttggg
    10921 ataattctta aagaagtatt agaaatagtt ggtacttcat t
//
LOCUS       DACCXW010000099        10870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCXW010000099 DACCXW010000000
VERSION     DACCXW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.100"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..132)
                     /locus_tag="IVY71_002159"
     CDS             complement(<1..132)
                     /locus_tag="IVY71_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7986199.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="IVY71_002160"
     CDS             542..841
                     /locus_tag="IVY71_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7986200.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="IVY71_002161"
     CDS             914..2275
                     /locus_tag="IVY71_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7986201.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="IVY71_002162"
     CDS             2297..2557
                     /locus_tag="IVY71_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986202.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="IVY71_002163"
     CDS             2571..3323
                     /locus_tag="IVY71_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7986203.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="IVY71_002164"
     CDS             complement(3417..4022)
                     /locus_tag="IVY71_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7986204.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="IVY71_002165"
     CDS             complement(4075..5049)
                     /locus_tag="IVY71_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7986205.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6206)
                     /locus_tag="IVY71_002166"
     CDS             complement(5427..6206)
                     /locus_tag="IVY71_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7986206.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="IVY71_002167"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="IVY71_002167"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7986207.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="IVY71_002168"
     CDS             7697..9094
                     /locus_tag="IVY71_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7986208.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="IVY71_002169"
     CDS             9175..9615
                     /locus_tag="IVY71_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7986209.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="IVY71_002170"
     CDS             9763..10089
                     /locus_tag="IVY71_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7986210.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="IVY71_002171"
     CDS             10090..10839
                     /locus_tag="IVY71_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986211.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3470 a         1917 c         2240 g         3243 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt
//
LOCUS       DACCXW010000100        10096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCXW010000100 DACCXW010000000
VERSION     DACCXW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.102"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..223)
                     /locus_tag="IVY71_002172"
     CDS             complement(26..223)
                     /locus_tag="IVY71_002172"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986212.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(369..1247)
                     /locus_tag="IVY71_002173"
     CDS             complement(369..1247)
                     /locus_tag="IVY71_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7986213.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1286..2404)
                     /locus_tag="IVY71_002174"
     CDS             complement(1286..2404)
                     /locus_tag="IVY71_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7986214.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2401..4500)
                     /locus_tag="IVY71_002175"
     CDS             complement(2401..4500)
                     /locus_tag="IVY71_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP7986215.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4551..5372)
                     /locus_tag="IVY71_002176"
     CDS             complement(4551..5372)
                     /locus_tag="IVY71_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7986216.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5369..6178)
                     /locus_tag="IVY71_002177"
     CDS             complement(5369..6178)
                     /locus_tag="IVY71_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7986217.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6199..6693)
                     /locus_tag="IVY71_002178"
     CDS             complement(6199..6693)
                     /locus_tag="IVY71_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7986218.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6705..7193)
                     /locus_tag="IVY71_002179"
     CDS             complement(6705..7193)
                     /locus_tag="IVY71_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7986219.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7270..9966)
                     /locus_tag="IVY71_002180"
     CDS             complement(7270..9966)
                     /locus_tag="IVY71_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7986220.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3068 a         2270 c         1661 g         3097 t
ORIGIN      
        1 tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta
       61 tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact attaccacag
      121 acataaagta acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg
      181 tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta
      241 atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc gctttatcca
      301 acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca
      361 ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt
      421 attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca
      481 acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt
      541 ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat
      601 gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc
      661 tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa
      721 tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata
      781 tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct
      841 ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca
      901 acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa
      961 tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg
     1021 atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg
     1081 atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca
     1141 actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact
     1201 gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc
     1261 cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac
     1321 gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt
     1381 gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt
     1441 cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt
     1501 cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat
     1561 ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac
     1621 gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg
     1681 ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc
     1741 cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac
     1801 cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc
     1861 aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg
     1921 tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg
     1981 tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca
     2041 acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt
     2101 caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa
     2161 aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc
     2221 cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt
     2281 tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat
     2341 tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac
     2401 tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt
     2461 cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt
     2521 acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt
     2581 atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat
     2641 cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac
     2701 gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc
     2761 tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct
     2821 cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc
     2881 tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg
     2941 atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc
     3001 ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat
     3061 taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc
     3121 atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca
     3181 aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc
     3241 tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc
     3301 ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg
     3361 ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac
     3421 ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag
     3481 taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc
     3541 aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc
     3601 aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc
     3661 aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga
     3721 aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag
     3781 ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga
     3841 ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct
     3901 tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg
     3961 ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc
     4021 gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag
     4081 tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg
     4141 caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac
     4201 aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac
     4261 ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat
     4321 cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc
     4381 ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc
     4441 gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat
     4501 cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt
     4561 ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg
     4621 acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta
     4681 aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc
     4741 aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag
     4801 atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat
     4861 ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata
     4921 ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc
     4981 gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc
     5041 aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt
     5101 tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg
     5161 gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg
     5221 tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc
     5281 ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc
     5341 tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa
     5401 gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta
     5461 gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg
     5521 gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac
     5581 tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg
     5641 ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc
     5701 caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa
     5761 agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag
     5821 cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg
     5881 ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg
     5941 ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga
     6001 cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca
     6061 tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca
     6121 tttcatgtcc agcaactcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa
     6181 tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat
     6241 cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg
     6301 aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg
     6361 tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc
     6421 aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg
     6481 cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga
     6541 tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc
     6601 tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat
     6661 taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc
     6721 tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta
     6781 tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc
     6841 agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt
     6901 ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc
     6961 tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca
     7021 taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga
     7081 aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct
     7141 ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta
     7201 atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt
     7261 ttagacactt tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata
     7321 agcattgact ttaaaattat atttcatctc cattgattga aaaatacttc tagaaacata
     7381 ataaaattca tcttcatctg gtttcaaatc ataattcggt tcttcagatt tttctgtccg
     7441 agccaagaat aaacgttcca tcatcaatgc cgtatgcata tagagattta gttttacacg
     7501 gccatttaaa gtgaaatgat agtaattttc atatttagaa atgactgttt ccacttcact
     7561 catcacgatc gttgggttca gaaagttcaa acggtccgca attccttcca acgaaaagaa
     7621 ttttactagc tcctgaatca agcggttaaa ttctgccggc ttcatttcag gactcaaaat
     7681 atgctccaaa ttggctgctc catcaccgtc taagatgtca taaacattaa tattaggaat
     7741 tgaaaaggaa cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt caaaataagt
     7801 ttcatcattt tggtcgatta attcacgtag ttctctgtag tccttcgtga aaacttcgat
     7861 ggattcatcc gataaatgat gacgaaaaac atctttcaat ttgtccgaaa tccccaaacc
     7921 cgacatacag gaaatgataa tgttgctact ttgagaaaag ccttcaaagt attgaacatt
     7981 gattgaatac ttgtctgctg catcctccgc aatctgttta aatggcgcat tcatcgtcat
     8041 cttcaagcca atatcaagtg caatggaagt cgtcaaatta ttcagaagaa gtaactcccc
     8101 ttttaaatcg ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat ctacgatcag
     8161 cactagacta tctgtattca cttgccgttt cacgaaattc cttgtcgatt cgacaatctc
     8221 tgacacatta ctctctactg gcatatccaa cgcttcaaaa acataattgc cgcataattg
     8281 attcactact gcttgaatgc tgctagccgt actatctccg tgagcaagca acaggccctt
     8341 cagctcgatg gattcatcta cataatcaga taaagtcacg acaataagca gtcttagaaa
     8401 gttctcgtcc ttttctgaga gcggtggtaa atgctccaag aactgattac ataagtaagt
     8461 cgtccgaggt agatttgcag ctaatgtacg atttatcccg gttaagtctg tcttatagct
     8521 tggggaaatg acataggatc tgctaataat ttctgcaaca ggtccactcg tcgtcaatcc
     8581 gtacgttttt cctaaaacgg atacccagac ctttctaaac atctgaatca tcaattttct
     8641 actcattgat aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta cttcattctt
     8701 tatcgaagaa aaattttcat tgaaattacc ttcatttatc agctgagtaa ttttttccaa
     8761 aatcgaatca taatcaaacg gtttgattcg atccgttttt atcttttgat ttttctttat
     8821 caataaacta gcaatcggca agctttctac ttcggattcc agcatattta actgaatcaa
     8881 gatctcattt ttcccaatcg ttaatggttc ttcttgtgtt cgactataca catccgcaca
     8941 actgatttta atgatattct tcagcttacc aatattccca ctaaagggat gagataacat
     9001 taaagacaag gcttctcttg aaacaataat atctttttgt aaaacatccg cttcattttg
     9061 aaaaaataat tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa aagttggcaa
     9121 cataacacta atttgaatac gccgatcaaa ggtgtctaat aaataatttt cacctttttc
     9181 cgtcgtggcg aaaataaaac gaaccttagc agaatgccaa tttacatttt caccgactgg
     9241 acgatagttt ccagtatcaa taaatagaaa gagcttttct tgattttctt tcgataggcg
     9301 atggacctca tcgagaaaaa gatagccgtt gttggccgtt gccaataaac catcggtatc
     9361 cttttcagcc cccgtaaagg cccccttctt gtatccaaac agtgttgccg aaagcagttc
     9421 gggattgtcc gcataatcgg cacagtttaa gatagcaaat ggtgcatctt tttcaataat
     9481 ttgctcatgg atagcgtatt caaaaatttt tgtggctaaa aaactttttc caacacccgt
     9541 ggctccattt attaatacac tgagtccgtt aggcggatat ttgacagcgg ctatacattt
     9601 ttgaatgacc tctctcagac ttccgtcatg accaactaca gaatgaaagg cttcgtttat
     9661 ttgctccgta ttatctacaa ccgcatattc atctaaaggc ttccacaaaa caggctttcc
     9721 cggtaatttc tcgacctttt tatcttccac taatctcgtc aaataatggc tggcattctg
     9781 acgagagata cctagattct tcgaaatttt ctcggcagtg acagaagaca tattccctgt
     9841 tagtcgtatc agctcatcaa aaactcgatc tttagcgctc tttttcattt tttcctccag
     9901 tttaatgtgt ctaaaagcat aataaactat cggctgcaat atgtctattc tatatttgta
     9961 ttccaatttt tactttgaaa cacaaatagt cgaatatttg cctcagatat ctgacaaaat
    10021 aatttatcta ccaatttagt ttttatctaa tatgattttt atgttttttc atagctacaa
    10081 atattgaact ggattc
//
LOCUS       DACCXW010000101        10082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCXW010000101 DACCXW010000000
VERSION     DACCXW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.103"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(109..2229)
                     /locus_tag="IVY71_002181"
     CDS             complement(109..2229)
                     /locus_tag="IVY71_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7986221.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="IVY71_002182"
     CDS             2459..3637
                     /locus_tag="IVY71_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7986222.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="IVY71_002183"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="IVY71_002183"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7986223.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="IVY71_002184"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="IVY71_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7986224.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="IVY71_002185"
     CDS             complement(4990..6243)
                     /locus_tag="IVY71_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7986225.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="IVY71_002186"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="IVY71_002186"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7986226.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFISGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="IVY71_002187"
     CDS             7821..9167
                     /locus_tag="IVY71_002187"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7986227.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="IVY71_002188"
     CDS             complement(9475..9861)
                     /locus_tag="IVY71_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7986228.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10082)
                     /locus_tag="IVY71_002189"
     CDS             complement(9910..>10082)
                     /locus_tag="IVY71_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7986229.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         3215 a         1839 c         2053 g         2975 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt atttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tg
//
LOCUS       DACCXW010000102        10075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCXW010000102 DACCXW010000000
VERSION     DACCXW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.104"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..704
                     /locus_tag="IVY71_002190"
     CDS             <1..704
                     /locus_tag="IVY71_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7986230.1"
                     /translation="LRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVR
                     REIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIR
                     ENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVH
                     AMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKL
                     AKAEQYRRSLELSKD"
     gene            776..1897
                     /locus_tag="IVY71_002191"
     CDS             776..1897
                     /locus_tag="IVY71_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986231.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(1979..3751)
                     /locus_tag="IVY71_002192"
     CDS             complement(1979..3751)
                     /locus_tag="IVY71_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986232.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3754..5466)
                     /locus_tag="IVY71_002193"
     CDS             complement(3754..5466)
                     /locus_tag="IVY71_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986233.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5581..6249
                     /locus_tag="IVY71_002194"
     CDS             5581..6249
                     /locus_tag="IVY71_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7986234.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6316..7104)
                     /locus_tag="IVY71_002195"
     CDS             complement(6316..7104)
                     /locus_tag="IVY71_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7986235.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7187..8659)
                     /locus_tag="IVY71_002196"
     CDS             complement(7187..8659)
                     /locus_tag="IVY71_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7986236.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8789..9982)
                     /locus_tag="IVY71_002197"
     CDS             complement(8789..9982)
                     /locus_tag="IVY71_002197"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7986237.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
BASE COUNT         3323 a         2039 c         1665 g         3048 t
ORIGIN      
        1 aattaaggaa gggtctttta gagtttaaac ttcaagaact agaacttcat tactcttatt
       61 tagtgaaaat taccgaagaa aaagaacaac aaaaagcaat aaaagaacag atgatagaag
      121 aggaaaaagt tcgtagagaa attgatcgtg aaaagaaacg tattgataaa gaacaacgtc
      181 aattcaattc tgaaatttcc aaattaatcg catatatgca aaaagctaat gcagatgtgg
      241 aaaaagaatt atatgcaaat aaaattcaag aactggaaga aaaattaaaa gaattagaag
      301 ttatacgtga aaatgtatta caaagagaat taaatactag agccggttat gtgtatgtaa
      361 ttagtaatat tgggtctttt ggtgaagaca tatataaaat tggtatgact agaagattag
      421 aacctatgga tagaataaaa gaattgagta gtgcatcggt tccatttgaa tttgacgttc
      481 atgcgatgat tttttcagag gacgctccgt ctttggaaac taaattgcat aatcatttta
      541 gaaaacaaga agttaataaa attaatcaaa gaaaagaatt tttcaaagtt tcactagacg
      601 aaatagagaa agttgtttta gaaaattata atggaacagt aacatttaca aaattagcta
      661 aagctgaaca atatagacga tctttagaat tgtcaaaaga ttaaatatat aaaaagcctt
      721 cgggcttttt cttttaacaa ttaataggaa cgtatattcg aaaggaatag aaataatgtg
      781 gttagaaaaa ataaatgaca aaaaatttaa gtacaccgaa cgttatacgg atcccttaac
      841 tgaaaaaaag agaaaagtgt ccgttacttt atctagcaat tcacgccaag cttggaatca
      901 agctaactta cttttgaatg aaaaaatcgc agaaaaaata aaacgaaatg aagaattacc
      961 actaactttt ggagaattaa aaactaaatg ggatgaaaaa tacaagccaa ctgtaaaaga
     1021 aagttcctat cgaacaactc aggtttatct atcattaatt tcaaaatata taaaagatga
     1081 tgtacttgta aaaaacgtta atagtaatct tatacaagat atgctagatt actactattt
     1141 tgaaaaaaat ttatcatata attatgtaat acatttaaaa acttttacag gaatgatttt
     1201 taaattcgcc aaccgtcgat atggattaaa atataatccc gtaaatgggg tttctttagc
     1261 aagaaaacct aagacaaacg aagaaataaa aaaagaaaaa gatggttatt tatcaaaaga
     1321 agaagttcaa atgattgcta gtaaacaaaa aagttcacat caacagtcac gttattcttt
     1381 aattactaaa tttttgtttt taactggact acgttatggt gaattgatat ctttgactga
     1441 atctgattat gatggtaata aaattacggt ttctggtact tatgattacg agttaaaaat
     1501 taaaacaaca actaaaaata caggttctta tagaaccatt gaattatcaa gtaatgctaa
     1561 agaaatcata gatcaattaa ttgaagaaaa caaattaatt aagaataata aagataaata
     1621 cattttcatc tctaaaaatg gtaacccgat atctattcaa gcatataatc aatctttaag
     1681 agcagtatcc aaagaattaa acctagataa aaaagtttct tctcacatgt taaggcattc
     1741 ccacatttct ctacttacag aattaggaat cccattaaaa gccattatgg atcgtgtagg
     1801 acatgaagat tcaaaaacaa cattaaagat ttatactcat acaactaaaa atatgcaaaa
     1861 tcaactagta gaaaaactag gaaaaataga aatttaaatt gccccttttc tgccccttat
     1921 aaaaacaaac aacataaaat atataaaaag aaccccgtta taacgaggtt cttttaattt
     1981 aactttcaaa tacaaattga ttatgataaa gttccgcata aaatccattt tctgctaata
     2041 actcgtgatg actgccttct tcaatgactt ccccatcacg caagacaacg attctatctg
     2101 cattcaagat tgttttcagc cggtgagcaa tcacaaagct ggttcttcct ttgattgctt
     2161 catccattgc tttttggatc cttgcttctg ttaccgtgtc tacattactt gttgcttcat
     2221 ccaaaatcaa taaagatgga tttgtgatga tcgttcgagc aatactgatc aactgctttt
     2281 gacctgtact gaataagtta ttttcttcgg atatctctgt atcataacct ttttctaacg
     2341 tcatgatgaa gtcatgaata ttcgcttgtt ttgctgcatt gatgacttct tcatctgaag
     2401 cgtccggttt gccgaatgtg atattgtctt tgatcgttcc ggagaacaat acagattctt
     2461 gcaaaacgat tcctacatgt ctacgtaatg catcaagatc ataatctcgg atatctattc
     2521 catcgatgac tactgaacca tcattgacat cgtaaaaacg gtttagcaga ttcatgatcg
     2581 tcgttttccc cgatcctgtt ggaccaacaa gagcaaccat ttctcctttg tccacattga
     2641 tcgagacatc tttcaagact agtttttctg gagaataacc aaaatctacg tgctgcaacg
     2701 caagaccttt ttcaacacca tcaaatgctt tacagttttc tggtttgact tcatcttttt
     2761 catcaaatat ttcattcaat cgtcttgccc ctgtaatcgc taattggatc atgctgtatc
     2821 cagatgaaat ctgcatcagt ggttggtaat attgttgtga atactggacg aatgtcacaa
     2881 ctaagcctaa tgccaccgaa cgttccaaat caccatttaa tgctaaccag ccgccgaaaa
     2941 agataactat cgctgtattg accagtgaca ttccttgcat catcggaaat agtagaccag
     3001 aataagcttg tcctttgaag gttgcttcgc gaacactttg attatgctct aagaatcctt
     3061 caatcgtttc ttcttgtaag ccatttgtga tgatgacacg ttgtccgctg attttttcat
     3121 ccatataacc atttagtttc cccacttcat cttgctgaag atccacatat ttccgtgctt
     3181 tgcggataat caagacagct atcaaaacgg ctactggagt ggaagcaatt gtagcccaag
     3241 ctaactctac attttggcgg aacatcatga tcaagatccc gacaagtaaa acagcattcg
     3301 tgatgacttg aagcaaagct tggttcaaac tattttgaat attatctaag tcactggtaa
     3361 aacgacttaa aatttcaccg tcttgatgag aatcaaagaa acggatcgtc aatttttcca
     3421 atttgttgaa tagaccgata cgcattcggt tcgttgactt ccctactact tgtgtgaaca
     3481 aaatactata gataaagttg gctgcactag ctagtacata aaacagcaag agtttccata
     3541 ttacagaaac aaatttgctt ttatcgtcta cgccttgcat caagcctcct acgtaattag
     3601 ctaattcttg aaaagcttcc ccgatgtatt gaggtgcctt tacttgtaga taggtagcaa
     3661 tgatcactgt gataaagata aagaaaaagg agagtttgta ccgcttcaaa tagtgataga
     3721 aaaatttact tgcttttatt agatcagtca tacttactcc tccgttcctt tttgagtttc
     3781 aaagatttcc tgatagacac ggtttccagc taccaattcc tcatgggttc cttcgccgac
     3841 taatctgcct tcatccaata ctaagatgcg gtctgctttg acaacagaag caattttttg
     3901 tgcgatgaca atcgtcgttg ttcccttcaa atctcgatcg agtgcttctc ggaccagctt
     3961 ttcagagcga gcatccagtg cactcgtact gtcatctagg atcaagatct ttggattacc
     4021 gataacgcca cgagtaatgg ataaacgttg cttttgacca ccagaaaagt tactgctacg
     4081 ttcttctatt ggtgcttcgt aacgatcaga taacttttcg atgaattctt ttgcttgagc
     4141 aatagaagaa gcatgttcca tctctttttc tgttgcattc tttttccctt ggcgcaagtt
     4201 ttgagcaatc gttcctgaga ataaaattgc tttttgcaaa acaaaggaaa ccgtttgtct
     4261 taaactcttt tcatttactt gacgaagatc tacaccacct acttcaactt tgccttctgt
     4321 cggatcaaat agacgaggga tcaattgtgc tagtgttgac ttcccggcac cagtcgcccc
     4381 gacgatacca accatttctc caggtttgat agaaaagctg atatctttaa gcgtatctgt
     4441 atcctcacca ggataacgaa aagaaacatg gtcaaaagtg acagaacccg tcaactcttg
     4501 ttctggaacg tctaaataag taagatctgg ttcagcatct aaaatttccg caatccgctt
     4561 cagcgatacg gctgctcttg aagtcatcat catcatcatt ccgccaataa tgattgccat
     4621 cataatctgc atcaagtagt tcatgaatga agcaattcca ccaattaacg taggatcatc
     4681 tttaacaaga tcgctcacaa aaaagatcgc cccaaccaca gccaaattgg cagccagcat
     4741 gaaggacggg atcatcacag aaaataatgt accgactatg atgttgtgtt ttgttaagtc
     4801 ctcacttact ttgctgaatg acttcaattg gttttcttct tgtacaaaag atttcactac
     4861 acgaatccct agtaagtttt cttttgcaat gctattcaca cgatcaatca gtttttgaat
     4921 gatcatgaag tgcttgccca tccttgtaaa tgaaagtgcc gtaatcagaa ataccgtaac
     4981 gataagcgct acgatgatcc accataattg cggcatcgtg atcatagcta aaatgaaggc
     5041 accaataaat aagaatggaa ttcgaaaaag tgattgcaaa gaaatcatca ctaaattttg
     5101 gatttgcgtg atatcatttg ttagacgaac aacaagattt ccagcagaga actgttcgat
     5161 atttccaaat gaaaatgtct ggatctttcg aaatgcttct tctcggatat ctgcacttac
     5221 gccttgagct acttttgcag agaaaatcgt gttcgttaca cctgcggcta atcctagcaa
     5281 agccagactg atcaggtaaa taccaattgt tttcatttca tcattatctt ctgtaataat
     5341 agcttccaat acttgctgta gtaacttagg ttgccataat gctgctgcaa ccatgactgc
     5401 tacggacagc aaggaaataa aaacagctac tttatatttt ttcgcatgtc gtaagattaa
     5461 tttcattcgt tgattacacc cctttgttta aattgacagg actaatttaa cactgtaaaa
     5521 ttagaccgac taacaattta ccatgtttaa aaagataatg caatacacga ggttgataaa
     5581 atggaaataa agctttctca agcaatcatc attcaggccg cctttgatat cctggctgaa
     5641 acacaagctc tttcgcagct ttccatgcga aacatcgcaa acagaattca tgttcaagca
     5701 cctgctttat attggtattt caaaaacaaa caacagctct tgcaaataat ggctgaaaca
     5761 atggaagaag aattagcgct tccagatcca actttgcctt ggaatgaaca gttgctgcag
     5821 tatatggaaa attactatga cctttataca cgtttccctt gtggggcaga attagaaatc
     5881 aatactgtgc ccgcttttcc gagtcgatta gaacatttgg agcaaatgat ccaattactt
     5941 gttcgtgaag gattttcagt cacccaaagt catcaggcca ttgcctctct ccacaattta
     6001 ttgattggac agttaatgga tcagcagcat gaacagcagc ttcgacaaaa aattatggac
     6061 ggaaatgaat ttttgaaaga aacagttcta tttatgcgta attatgtagt tgaaaatcaa
     6121 ttagagggat ttaaagaaag tatgcagtcc catgcagaat tgaatgaaaa agcactcttt
     6181 ctaacgagtg cacaaaccta tattgaaggc ttatgccaac aaaataaaag aagagaaaac
     6241 gagcagtaaa aaagctccta tagagatcac tatgtttgtg atctctatag gagctatctt
     6301 tttaagactc tttttttatt tttcttccat tagccacgcc aatgcacgtt gaatccattg
     6361 gtcccagaag atccagttat gatcgccagg tccttgttca aaggtgaccg ccaagccttg
     6421 ttcctgcata gcagatacca tttcctgatt tgcctgataa agaaaatcag attccccaca
     6481 agccatataa aatcttggaa ctggttttcc gctttttact agttctttca ataagtattg
     6541 cggatcattt tttgaacctt cgatttgatc caatggtcca aagatccctt cccaaaaaga
     6601 tgctttacga accatcaaaa gatcatccat tcgatttcct acagaaacag cgcctgaaag
     6661 agagattact gctgcgaaat tttctgattt tgcaaggcct agttttaacg cgccatatcc
     6721 gcccatcgat aatccagcgg catatgtttt ttctcttttt cttgttaatt gcgggaataa
     6781 ttcatgacaa atctcaggta gctcttctgc aatgaatgtc caatacttca tgtcataaac
     6841 tgtatctgtg taccagccaa gatcagtaga tggcatgatg acagctaatc cgtagtttgc
     6901 tacataacgt tcaatcgacg tacgtctttc ccatacacta tgattgccac ccattccatg
     6961 aagtaaatat aaaacaggta tatccgttgt ctcttgctct gaactggtgc caatcacctt
     7021 ttttgttgtt tgcggtagta tcacatttag attgacttcc atttctaaca cattcgaata
     7081 aatatttgct tgaataaatg ccatattttc gccttcctta gtaagaagtt atttttgaat
     7141 aactttaaat ttgaatgatt cggctacgta catatcttaa ccgaaactac tgttttattt
     7201 taccactttt ttcatgaacg aaaagcgtta agaccaaagc aatgatcacg agtaacaagc
     7261 caactagata aacggaatgt aacccatcat aaagaatttt tcttaatggt aacaaaatat
     7321 ccgcatcaat atatttagcc gtttgtggat tcaccagttt attcatgata tctgcatcat
     7381 ttgttaaatt tgctgctgca agttctcgtt tagttacccc atttatcaat aaaccaaaaa
     7441 tagaaaccat gactgtttga ccaattgttc gaactaacgt attaaaagaa gttgctacac
     7501 caagctggtc acgagataca ctattttgtg cactgacagt cgaggtagtc atggtcacac
     7561 ctaagcctat accgatgacc gttgatatag caaaaaacca taaatagtta cttctgaaag
     7621 gcataagata caaccaagcg cccccaatag ctgcgattag tagtccaatt ctcaagaccc
     7681 aatgagtttt catttgagtc atccacttgc ttgcaaggaa agaaccgacc atccagacta
     7741 gtgataatgg tgctaatacc agcccaccaa ttgctgcatt cagccctaat acaccttgca
     7801 tccacatagg gatataaaca tcgattccca tcaaaaaacc actagccaat gctgcagcca
     7861 agttgaccac aacaaatagt ggatttttga ataaatgcat catgatgaca ggatctttag
     7921 ctcttttttc ggcaaaaaca aaaataccta acattacgat actacctagc agcaaagcag
     7981 aaacaagcgg actcattcct tcgtctccta ataattggaa ccctaacagc aatgagacaa
     8041 gcatggccat caaactaaaa ctaccaaaaa tatccattgg tttagactca tgtatgattt
     8101 ttggttcatt taaatatagc caaatcaaaa taattaatag aattccgatc ggcacattga
     8161 taaaaaagat ccagtgccag ctgatcgttt caacgatgaa tcctcccgca agcggtccaa
     8221 aaacactggc aatcccccag gctgaactat tcaaacctaa aacttttgct cgcttttcaa
     8281 tagaataaag atcgccgatg atcgtcaaag ctactggcat cattgcccca gcacccatcc
     8341 cttgaatcgc acgagcagca atcaacccaa tcatgctttg agataaaccg caaaataacg
     8401 aaccgataat aaacaataat gtcccaaaaa taaagattgg tttccgtccc actttatccg
     8461 caagttttcc gtaaatcgga gtaagcatag cgtttgtcag caaataaatc gaaaataccc
     8521 agttcatgat ttccattcca tgtaatgaac cgacaatcgt tggcatagcg gttgttacga
     8581 ttgtaccttc aatagccgtc atgaacgttg caatgaaaat actcactgtg accaaagtaa
     8641 catttgtttc ttttttcatc ttattcctcc ctttcattat gacaggatct aaaaatagat
     8701 aaataattac tatctttttc aagaggttaa ctgccataaa aaaagctgga acaaccacca
     8761 cttggcgatc gttccagctg aatataactt attttgctaa acttgctttc aacgcatcta
     8821 ctttgtccgt atgttcccaa ggcaaatcta catctgtacg accaaaatga ccgtaagcag
     8881 ctgtttgttt gtaaatcgga cgacgcaaat caagcatctc gatgattcct gctggccgaa
     8941 gatcgaagtt ttctctcact gcagcaatca gttcttcttc cggaaccgtt ccagtaccga
     9001 aagtattgat tgagatcgaa acaggttgag caacaccaat cgcataagct agctgtactt
     9061 ctgctttgcg agcaagccct gctgcaacaa tgtttttagc aatataacgt gcagcatagc
     9121 tggcagaacg gtcaactttt gtcgcatctt taccagaaaa agcaccgcca ccatgacgag
     9181 cataaccgcc atacgtatca acaatgattt ttcttcctgt taatccggca tccccttgag
     9241 gaccaccaat gacaaatcgg ccagtcggat tgatataata tttcgtttga tcatctaata
     9301 attcagctgg aataacttcc ttcactactt tttcgatgac atcatgacga attgtttcat
     9361 tatctactgc atcatcatgc tgtgttgaaa taacgattgt atctacgcgt tccggttgtc
     9421 cttgatcatc atattcaacc gttacttgag attttgcatc tggacgtaaa tacgttaatt
     9481 catttgattt acgcaaatct gccaagcgtc gtaccagacg atgactcaaa gcgattggta
     9541 aaggcatcaa ttctggtgtt tcatctacag caaacccaaa cattaaacct tggtcaccag
     9601 caccaatctc atctaatacg tcttttttat cttcatcccg gatctcaagt gcttcatcaa
     9661 ctccttgagc aatatcagga gattgttcat caatcgcaac caatacagct gctgtatctc
     9721 catcaaatcc gaattttgca cgtgtataac caatttcttt tattgtttcc cgtacgactt
     9781 tttgaatatc tacatatgcc gtcgtagata tttctccaaa tactaaaaca agaccagttg
     9841 ttacagacgt ttcacacgct actcgtgctg ttggatcttg ttttaaaatt gcatccaaga
     9901 ttgcatcact gatttggtca gcaattttat ctggatgtcc ttcagaaaca gattctgatg
     9961 taaataagtg tctttctacc attataatga ttccccctag ttaaattcgg ttacaaggca
    10021 tctccgtcct gcggatcctt tcgggaactt gcaacgaacc aagtatagca gataa
//
LOCUS       DACCXW010000103         9975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCXW010000103 DACCXW010000000
VERSION     DACCXW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.106"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            278..787
                     /locus_tag="IVY71_002198"
     CDS             278..787
                     /locus_tag="IVY71_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7986238.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IVY71_002199"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IVY71_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7986239.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IVY71_002200"
     CDS             2322..3302
                     /locus_tag="IVY71_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7986240.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IVY71_002201"
     CDS             3424..4842
                     /locus_tag="IVY71_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP7986241.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IVY71_002202"
     CDS             4913..6382
                     /locus_tag="IVY71_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7986242.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IVY71_002203"
     CDS             6392..6859
                     /locus_tag="IVY71_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7986243.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IVY71_002204"
     CDS             6895..7767
                     /locus_tag="IVY71_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7986244.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>9975
                     /locus_tag="IVY71_002205"
     CDS             7848..>9975
                     /locus_tag="IVY71_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7986245.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTL"
BASE COUNT         3460 a         1444 c         1987 g         3084 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttag
//
LOCUS       DACCXW010000104         9908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCXW010000104 DACCXW010000000
VERSION     DACCXW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.107"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            91..3318
                     /gene="fss3"
                     /locus_tag="IVY71_002206"
     CDS             91..3318
                     /gene="fss3"
                     /locus_tag="IVY71_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7986246.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3431..3745
                     /locus_tag="IVY71_002207"
     CDS             3431..3745
                     /locus_tag="IVY71_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7986247.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3758..4132
                     /locus_tag="IVY71_002208"
     CDS             3758..4132
                     /locus_tag="IVY71_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7986248.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4142..4477
                     /locus_tag="IVY71_002209"
     CDS             4142..4477
                     /locus_tag="IVY71_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7986249.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4560..5900
                     /locus_tag="IVY71_002210"
     CDS             4560..5900
                     /locus_tag="IVY71_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7986250.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5978..6652
                     /locus_tag="IVY71_002211"
     CDS             5978..6652
                     /locus_tag="IVY71_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7986251.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6885..7319
                     /gene="dcm"
                     /locus_tag="IVY71_002212"
     CDS             6885..7319
                     /gene="dcm"
                     /locus_tag="IVY71_002212"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7986252.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7320..8027
                     /locus_tag="IVY71_002213"
     CDS             7320..8027
                     /locus_tag="IVY71_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7986253.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            8017..8307
                     /locus_tag="IVY71_002214"
     CDS             8017..8307
                     /locus_tag="IVY71_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986254.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8566..9750
                     /locus_tag="IVY71_002215"
     CDS             8566..9750
                     /locus_tag="IVY71_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7986255.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9765..9884
                     /locus_tag="IVY71_002216"
     CDS             9765..9884
                     /locus_tag="IVY71_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7986256.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         3282 a         1670 c         2245 g         2711 t
ORIGIN      
        1 gtcaataaaa attttaaaaa gactttataa acaaagatgt tcaagcgagc atcttatttt
       61 tttgtgctta atttgaaagg agaacatttc atgtataaaa aacaaaaaaa gaaattatgt
      121 gtattaacta ttttgagttt ggctgttcaa gcaattgttg gagcaattgc tcctacgatt
      181 acttttgctg atgaaattac ccatccacaa acggtaaccg tagaactaga tttagcacat
      241 caatatgctg ttgaaggaac ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg
      301 cattatgcag tgtacaacgg ctttaaacaa gatattttct gtattgaacc aggagtcccg
      361 atttacaatg agttcactcc tggctatgag aaaaacccgt taccagatat gtccgataaa
      421 gccaaattag tttccgtttt atggaaaaaa gtaggtacag atgcagatac tcatattgtg
      481 gcacagaaga tgatctggca agaagttaat gggtataccc ttcactcaat aaaacgatca
      541 gatggtagtg ccgtaaatat cgcagccatt gaggcaaaaa taaatcaagc cattgacgat
      601 tatcagaaga aaccaacctt ccataatagt acggctaaaa cagtattagg gcaatcgact
      661 actgtgacgg atacgaataa tctgaatttg tcagagttcg atgaggttgt ggaaaatgcc
      721 gcaaacattg attatcgtgt gaatggtaat cagttgataa tcaccccaaa tgccaattca
      781 aaagaaagtg gtgtgctgac gcttaagaaa tctgctggta ccggaacacc tgtcgcctat
      841 aaaaaggctg gccaacagac tttaatggcc ggggcgattg ataaacctaa tacctacaca
      901 atcaaaattg atgtggaaac ggagggttct ttaaagatca aaaaagtcga taaagaatcg
      961 ggtgatgttg taccaggaac ggttttccat ttagactttg gaaaaacttt acctgcaaaa
     1021 gacgtgacta ctgacaaaga aggcattgct acattggatg agattcctca tggtacaaaa
     1081 gtaaccatta ccgaaaaatc agtaccggcg ccttatacga ttgataccac ccccatgact
     1141 accaccatta aagcaggcga aaccatttac gtgacttcaa aaaacgcacg ggaaaaaggg
     1201 caaatcattc tggataaaag cggagttgaa acagggagtg atctttggaa tgacaattat
     1261 tccttagctg gtaatacgtt tgccattcgc aaagacagtc caaccggtga aatcgttcaa
     1321 gaaatgacca cggatgaaaa cggtcatgcg gaaacaccaa aagagattgc taatgcgttg
     1381 gaattgggaa cctattatgt gaccgaaact aaagctagtc atgggttcgt gaatacgttc
     1441 aaacctgtaa aagtcgaatt gaagtatgcc aatcaaaccg tagcccctgt taccagtaac
     1501 gtaaaagggc aaaatcaaga agtcactggc gaaaccactt tgacgaaaga agacaaagat
     1561 actggggata aagcacaagg taaggcagtc tttgagggtg ctgaatacac cttgtttact
     1621 gctaaggatg gaaaagctgt taaatggagt gaggctttta aaccagagtt agcgaaggga
     1681 acgaaaggtt ctgatgaaac ggtcactttg actttagacg aaaagaacca agttgccgtt
     1741 aaacatttag ctatcaacga gtattattgg caagaaacaa aagcgcccga aggatatagc
     1801 cttgatgaaa caaagtatcc tgtatccatc aaaaaggtgg atgatagcga aaaaaatgcc
     1861 gtgattaccc gaaatgttac ggcaaaagaa caagtgattc gctttggctt tgatttcttt
     1921 aaatttgctg gatcagctgc gggcactacc gaaactggct tcaatgatct gacctttaaa
     1981 gtgtcgccat tggaagggac caatgaaatc acaggtgccg aagatgaagc gaccacagct
     2041 tataacgagc aattagggtt tgatggttat ggcaagtttg aaaatcttcc ttatggggat
     2101 tatttacttg aagaagtaga agccccagaa ggttttcaaa agattacgcc attagaaatc
     2161 cgttctactt ttaaggaaaa caaagaagac tttgtgaaga gtgagtatgt ctttaccatt
     2221 accgaacaag accaaaaaca accaattaaa acggtgaccg ttccttacga gaagctgacg
     2281 aacaaagcat tttctgttag tttgaaccgt ttaatgctct atgatttgcc tgaggaagaa
     2341 gatagtttaa cttctcttgc gacttggaaa gacggaaaca aagaattaac ttctctggat
     2401 tctaccgagc tagttgataa attgagttac aatctacatg aaatcaaaga agactggtat
     2461 gtggtagcgc aagccattga tgtggatgcg acaaaagctg cccaagaaaa agatgaaaaa
     2521 gccaaaccag tggtgattgc agaaacaagc gcaacgttgg cgaacaaaga gaaaacagga
     2581 acttggaaaa ttcagcataa attaaccgcc gaacaagttt taaataaaac tattgtctta
     2641 ttcaactatg tgtatgaaaa taaggaagct tttgaagcag gcgataagcc agtagcgaag
     2701 gatgttagct tgaataacca agcccaaact gtcagttgta cagtggaaca ccatgtttct
     2761 atccaaacaa aggcccacct agaaaacggt tctcagacct ttactcatgg tgatgtggtg
     2821 gatatgtttg atgatgtgtc gattactcat gatgtactgg atggctcaaa agaagctttc
     2881 gaaacaattc tgtatgcttt attaccggat ggtacaaaca aagaaatttg gaaatctggc
     2941 aagattgatt atgaagtaaa tgataaagaa ttcaccaaaa ctgtgcttgc gaaaaaagta
     3001 gataccggaa aatatccaga aggaaccaag tttacttttg cggaaatcaa ttacgataaa
     3061 gatgggaata tcaatggaaa acacaatgaa gatttgaaag aaaaatctca aaccttaaca
     3121 ccaaaagaag ttccaaccat actaagtacg ccgaaacaac cggaaacacc aactgttcca
     3181 agtggctctc aagaatctag tcccacagtg aagaccttcc ctcaaaccgg ggagaaaaat
     3241 tccaatgttt tactgttcat tggttttact ttgatttttg cgacggcggg ctattatttc
     3301 tggaatcgcc ggaactaagg tgatgagatg atacaagtaa atcctaagaa ccaacgtgcg
     3361 cgaccgcctc ctaaaaaagt agtcgcgcta tttttgtatc caaaactaac aatgaaaaga
     3421 ggaaaagaag atggaattga aatttgtcgt gccagatatg gctgaaacct ttggaaagtt
     3481 aagttatgcc ggtgaaggag aaattataac agaaggatat ggacggaata ctacggtgat
     3541 tggtcgcagt tatcatttgt attccagtaa gcaacgggcc gatgatattg aggtggtagt
     3601 agctgcggaa gccggtgaaa aggattttga tcaagaccaa ccgctaaaag ccgtgaatcc
     3661 ccatttggtt gccaaaggct atgagattga gaatcgtggg tttactgatt acgtgttgta
     3721 tgttgatgat ttagtgaaag cataggagga aaagaagatg agattaacag aaggtattgt
     3781 cgtagattca ggtttaacat ttgggaagtt gcgtttttcc gcattacgcc gagaagtacg
     3841 gaagcaaaat gaggatggaa caattggtaa tgaggtaaaa gaacgaacct ataatttaaa
     3901 atcctctgca caagggcgaa tgattcaagt tagtatccca gctaatgtac ccttacgcga
     3961 gtttgcttat gatgcagagg tagagttggt caatccaatc gtggataccg ttgccaatta
     4021 tgtttttcga gaaggaacta ccgttaattg gttcattaag gcggatgatt tagtcttgaa
     4081 gcggcagccg aatcaaggaa atcctacgaa ccaaaatgaa agaaagaaat aggtgaagat
     4141 tatggaaatt aatgttgaac atattttaga ttgtctcgat caatatggaa aaggcgagct
     4201 gactgaggaa caactcgtta aagcattaac ctctgatgaa aaaatgtttc tgattataca
     4261 tcaaggttta cttggagaaa atagcgacag tgaagaagac tctgatgtac tagactggtt
     4321 aggagaagaa gagtctttct ttatagttgt cgaagtaaat gaaaacttat gtcggcaggc
     4381 agaatccatt ttggaagaaa ttggtgtgga gatgccggat gctattgaag gctttttaaa
     4441 tcaattggtt gaaacgaaac aactcccctc ctcgtagcag taaatgacta gcataacttt
     4501 ttgaaattgc ctaatgaaat cctattgggc tttttcgatt ggaggtggaa accatcaaaa
     4561 tgtacaaagg acatcgcata cgcgcaggag atcagcactt ggtttatcac tttgttcttg
     4621 gctggctact cgctctgttc atcggttgga tgagcgtctt ttattttcaa gagtttagac
     4681 aatttgatat ttctaagcta tcactttcta ctattgaaat cgtttggtct ataaaagatt
     4741 tagtatgtct gcttggaagt ctcgcttttt caggagctat gatattgctt tatattcact
     4801 tttttcttga tcattggcga agcttgtggc atcgacaaaa gctagctcgg atgattctag
     4861 agaaccattg gtatgaagtg aaacaaactc aaagtgaagg ctttttcaaa gatttgaata
     4921 gtagccgaac cagagagact atcagttact tccccaaaat ctgttatcga atgaaagatg
     4981 gattactgtc tattcgcgtt caaatttcac ttgggaaata ccaagatcag cttttaaaat
     5041 tggaaaagaa gttagaaagc ggactgtatt gtgagttagt agaaaaggaa ctcaaagact
     5101 cctatgtgga gtatacttta ctgtatgata tgatcgccaa tcgaattggg atagacgaag
     5161 tagtggcaga aaatgggact ctgcgattga tgaaaaatca agtatgggct tatgattctt
     5221 taccccatat gttaatcgct ggtggcacgg gtggagggaa gacctatttc ctcctcacca
     5281 ttatcgaagc actattgaag tcagatgcag aattgtttat cctcgatccc aaaaatgcag
     5341 atttagctga tttaggtacg gtgatgcctc atgtttattc acaaaaggaa gaaatttctg
     5401 cttgtgtgga agatttctat gaacgtatga tggctcgtag caaggcaatg aaagaaatgt
     5461 ctaactacaa aacaggagag aattatgcct atcttggact tccacccaac tttttaatct
     5521 ttgatgaata cgtggcttat atggagatgt taacgacgaa agaaagtgcg gtgattttga
     5581 ataagctcaa acaaatcgtg atgttgggtc gtcaatctgg tttctttctg attttggctt
     5641 gccaaaggcc ggatgcgaaa tatttaggag acggaatccg cgatcaattc aactttcgcg
     5701 tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag gtcgaaaaaa
     5761 acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc agtgtgatta
     5821 gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca attagtaaat
     5881 tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa gtaacgtata
     5941 atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg attcttattt
     6001 agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag ggaaatcccc
     6061 cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa aatcagaggt
     6121 tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg attactttcg
     6181 cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag catcttcttg
     6241 gttggtagag caggcattta gatatgtctt agatcatggc tattctttta ttctggatgc
     6301 aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa aacattttcg
     6361 agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta cacaagaacg
     6421 tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt ttttcaaatc
     6481 aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc atatcataat
     6541 aaaagattat acgaataata tttccgaagt tcattatgct acggataatc tagaattatt
     6601 tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact gagaaagaac
     6661 aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg gaaaatgcta
     6721 ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat gaaaaacaac
     6781 ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat gagccaagag
     6841 caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga tttttagatt
     6901 tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat cattgtatcg
     6961 gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat gatacaagta
     7021 aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa tccctcggac
     7081 cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg ggaaagcggc
     7141 aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc accactcttc
     7201 tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac gaaggagggg
     7261 ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg gagtggtagg
     7321 tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt atcggacatt
     7381 ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca acatctgatc
     7441 aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt ggacaagtct
     7501 atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa atcgcactgc
     7561 cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa caaggtaatc
     7621 gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt ggtacaaaaa
     7681 tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac agtgtgaatc
     7741 tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt gttcatacct
     7801 tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg aagctaacac
     7861 ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga gattctcaag
     7921 tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt ccggtcattt
     7981 ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga gttgattgga
     8041 gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga acaattgcga
     8101 cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc atatttaatg
     8161 aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt tgagcggatt
     8221 gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa agcagagttg
     8281 gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt taggcgctga
     8341 ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca tggacggcgt
     8401 tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac acaaaaaata
     8461 agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt gtataggtgt
     8521 taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc acaacggaat
     8581 ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca aaataaattg
     8641 gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg tggcgtaacc
     8701 gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa tcccgatttg
     8761 cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga tgcacgaaaa
     8821 attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga ttatgccttt
     8881 tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc tcatgaagaa
     8941 gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga aactttttta
     9001 ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc cggtggcgtg
     9061 atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat acctgagcta
     9121 acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa aagttatcgt
     9181 tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt atatatcggc
     9241 agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca atatatcaag
     9301 ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg actgaaaaat
     9361 gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga gagcaccacg
     9421 ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa aaggcgaacc
     9481 aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa agagattcaa
     9541 ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct aggccgacaa
     9601 gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac aacctatatc
     9661 caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt ggagcaacaa
     9721 agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga attgtaaaag
     9781 atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc ttgatgaatg
     9841 caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat gaatttcgga
     9901 caaaattt
//
LOCUS       DACCXW010000105         9903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCXW010000105 DACCXW010000000
VERSION     DACCXW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.108"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            166..1038
                     /locus_tag="IVY71_002217"
     CDS             166..1038
                     /locus_tag="IVY71_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7986257.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1035..2993
                     /locus_tag="IVY71_002218"
     CDS             1035..2993
                     /locus_tag="IVY71_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7986258.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3325..4176)
                     /locus_tag="IVY71_002219"
     CDS             complement(3325..4176)
                     /locus_tag="IVY71_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7986259.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4299..4766
                     /locus_tag="IVY71_002220"
     CDS             4299..4766
                     /locus_tag="IVY71_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7986260.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5029..5511)
                     /locus_tag="IVY71_002221"
     CDS             complement(5029..5511)
                     /locus_tag="IVY71_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7986261.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5611..6474)
                     /locus_tag="IVY71_002222"
     CDS             complement(5611..6474)
                     /locus_tag="IVY71_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7986262.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7072..7947
                     /locus_tag="IVY71_002223"
     CDS             7072..7947
                     /locus_tag="IVY71_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986263.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8092..8244)
                     /locus_tag="IVY71_002224"
                     /pseudo
     CDS             complement(8092..8244)
                     /locus_tag="IVY71_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(8512..9843)
                     /locus_tag="IVY71_002225"
     CDS             complement(8512..9843)
                     /locus_tag="IVY71_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7986264.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         3116 a         1970 c         1718 g         3099 t
ORIGIN      
        1 tgattacgtt aacaataatt cgtttcatgt gactagatca tactaggcat gaaaaatctc
       61 tgaggaacat ggtttcccta ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac
      121 tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag
      181 atctttaatc aaaatgcagt attggtagat gacggtggac aggaaaaagt agctattgga
      241 aaagggattg gatttgacag aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg
      301 atatttgtca tggaaccaga aggtcaaatc aagttgcaaa atttacttaa tcagattgat
      361 gaacgctttt tatttgcagc agaaacgatc attgatcatg cggaaactgt tttgatggaa
      421 aagttgaatg aacatgtatt gatcgctttg accgaccaca ttgctttttc tgcggagaac
      481 attaataacg gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt gctttacagt
      541 gatgaattca gtattgctca atgggcagta gagtatctga caaaagagct tgggattcca
      601 tatacttatg acgaagcagg atatatcgca atccatattc acagcggacg aagcggtcgg
      661 aacaataacc atcgcagtat ccgtgaagtg acgattgtat ctgacatcat ccatttagtg
      721 gaatcagaat tggaaacaga tatccattca gaaccatttt cactgaatta ttcaagactg
      781 gtgaatcatt tacgactgct tctacaacga acccatgcgc aacaatatgc tgttttagac
      841 acggaaatcg tagaaatggt caaacgaaaa tacccagaaa gctataagat ttctaaaaaa
      901 atccgtgtgt tgctgaccaa agaataccag ctcgcgatca cgaaagaaga actgggttac
      961 ttggccatcc atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt taacaatcat
     1021 gaggaggaaa aaaattgaaa gcatatctac aacgaatggg acgttccctt caacttccag
     1081 ttgcggtctt acctgctgct gctttactag tagggatcgg aaactggtgg gcatcttata
     1141 gtaacgacat tgtcgctcat tttttacaag ctggcggtaa cgcagtttta ggtcaattac
     1201 ctttgctgtt tgccgttggg ttagcactag ggatgtcagt ggacaaatca ggtgctgctg
     1261 ccttggcagg tttggtcgca tttgaagttc cagtaaatgt tttaaaaact gattcagtag
     1321 ctactttatt gaatatcaaa ccagaatcag ttgatccatc ttttgctcag atcagtaacg
     1381 tatttattgg tattattgcc ggattgattg cggcagctct ttataatcgt ttccacgaag
     1441 caaaattacc gatggcttta tctttcttta gcggcaagcg tctagtgcca atcttggctg
     1501 cctttgtcat gttgctgatc agtgcagtgc tactgctcgt atggcctccg gtttactctg
     1561 ctttagtttc ttttgctaaa ttcattttga gtttaggctg gggtggtgca ggtctttatg
     1621 gcttcttcaa ccgtttactg atccctacag gattgcatca agcaatgaac tccgtgttct
     1681 ggtttgacgt agcaggaatc aatgatattg gtaaattttt ggcttctgaa ggaacaaaag
     1741 gcgtaacagg aatgtatcaa gctggatttt tcccaatcat gatgttcggg cttccagcag
     1801 gagcatatgc tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga
     1861 tgttagcagc agcttttgct tcattcttta ctggtgtaac agaaccactt gaattctcat
     1921 ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat tttcactggg atttctttag
     1981 cagtgagtgc attcttccat tggacagcag gatttgcctt tagtgccgga tttgtcgatt
     2041 acttcctatc tttgaaaaat ccgattgcga atcagccatt gatgttgatc gtccaaggat
     2101 tagtaacagc agtgatttac tactttggct ttaactttgc tatcaaaaaa ttccatctga
     2161 tgactcctgg ccgcgaggaa gcagatcttt cagatgacga tactgcaacg actaattcag
     2221 acaataaata tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga gccgacaatg
     2281 taacttcgat cgataactgt actacacgcc ttcgtttgca agtcaaagat acaggcctga
     2341 tcgatcaaaa taaaatcaaa gccactggcg tacctggtat gaagatcatc gacggaaaaa
     2401 atgcgcaagt catcgtaggt acagaagtac aattcgttgc agatgagatg gcaaaactac
     2461 atggtggtgc tgcaacacga cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg
     2521 ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg attccaatca
     2581 ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg tgacggctat gctgtattac
     2641 cagaaaatgg agaaatcttc tctccaatcg ctggtacgat cacaaacatt ttcccaacga
     2701 aacatgctgt cggaatccaa acagatgctg gtatcgaagt cttgcttcat atgggcatca
     2761 acactgttga cttaaaaggc gaaccattca ctttatatgt aaaagaaggt caaaaagtcg
     2821 caagaggtca attgattgct ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca
     2881 cggatatggt cgttgtattc acaaatggcg acaaagtcca aagcttagaa atcgaacccg
     2941 cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc caacaaagcc tgagccatcg
     3001 tcttccccag acttttttat cgataaacaa acaggagctg agacaaaaat cttgtctcag
     3061 ctcctttttc cgaataaaaa cggcgttctc ctgccaactc cgcggtaata agtccagcca
     3121 aacgtaacat atgaagagct attttttatt tgactcttct tattacttga tgcagaacag
     3181 cttttttaca acctctgata aacggtgttc aaaaaccagt ttatcggtat gaagtcaaat
     3241 ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttcatactca aaactaatcg
     3301 gctttttcac aacctcttta tacattattc ctgatccaat atttctttta atgctgcttc
     3361 aagagaagtt aatggttttc ccaatacttt ttctaaatca tcagaggaaa tagctaactg
     3421 atccttttta atatcatgtt ggatcgctaa aaacatgtca gctgcttctt gagggaatcc
     3481 ggcttctttt agattttcga caaacgctgc atcacttgct tcaataattt ctaacttttt
     3541 acctgtcgca cgctctaatg ccttggctaa ttcttcatag tcagttagat tgccggataa
     3601 ttctaagatt tctggagagt cactttctac tagtactttt gctgcagctt ctgcatattc
     3661 acgtttcaat gcccaacctg tttttccatt tcctgcagca taaacgaatt gaccagtttt
     3721 taaagcctgc ccaatgatag gtaattcatt ctctacatac caattattcc gtaaaaatgt
     3781 atgggaaata cctgaatcct taatcatttt ttcagtgaat tggtgatctg gcgctaatac
     3841 actgatcgag tgatcggcat ctgcaaaact tgtataagcg atataagata ccccagctgc
     3901 ttgtgctgcc ttcactacat tcttatgttc ggcttgacga tttcctggaa cgccagagac
     3961 aaaaagcaat cgatctattc ctttcaacgc ttctttcatt gagtctaaat catcataatc
     4021 accaatacgt atattaaatc cagcttcttt cagtgcttct cctttttctt cactacgtac
     4081 tagtacatag atttcatttt gtggaaccat cttttttaag aattccagcg cataaccacc
     4141 aaaacctcca gttgctcctg ttactaaata atgcatattt gccactccct attcttttta
     4201 tttgttaaga tttacagctg tacatttata aattacacct ttatcataaa gagtgtggta
     4261 agataagtca agcaaaaaaa atgaaaggat gacaactgat gaagtattct ctgcaattta
     4321 gtgatgcaat ccatgtactt gcctatattg aaatgtttca agatacccga tcactttcta
     4381 gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc
     4441 agctcaaaaa atccggtttg atctttacaa aaaccggaaa accgcagcct tcccttgcca
     4501 aaagtccaga tcagatttct ttactagatg tctatcgcag catcgaaggt aatacacagc
     4561 ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat atccaagaag
     4621 ctttagaaga aagctacgct cggctgcaga aaaaggcaga agaagaaatg gctaagatca
     4681 cacttgatag tctgatacat aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca
     4741 caggcctcgt agagagattt ttataattct ctctactgtg cctgcatttt tctgatcgga
     4801 atcgtctaca taaattcttt tattctccgg gggacaaaag ccttgtcatg cccctttttc
     4861 ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt
     4921 agctgaccct cggcattagt tctgaaaaat gcaaaatatg gaaagctatt tcccattttt
     4981 cctctctaat gctcaggtca aagcgctact ttcacgacct ctatattatc atggtttatt
     5041 gactaccggt atccagattt ctcccatata tttcccgttg gtaactccca tttcgactac
     5101 agcatttgga cccccaacat aggcgtaatc ttctacttct cttaaaacat gcccaaaggc
     5161 cgtattcgtc aaatcatttc ttaattcttc aggcgattct gctaaagcga ttacaacaag
     5221 atacgttcct tttgggaatg ggatgattcg ggtagtgttt ggaacatcct gactcgtttg
     5281 aacaccgaca tagtacatca ttttcccgtt atagggttcg ttgactgtaa agaaataatg
     5341 attttcagca gcttgtttca atgcatctat tgttccatta ccgataactt tttgctcaaa
     5401 tgcttgtttc tctgcagcca aaccgacaaa atccgtattg ctactcttca attccacacc
     5461 aatacccgtc accgtaaaat ctggtttttc ttcgatccta tactttgcca tttctcccac
     5521 cctttctatt atattaagtt catcttacat aaaattaaag ggaaatgaca cgattacgct
     5581 cttttttcta gggatatttt tattttttaa ctattttttt gctttcactg caatccaaat
     5641 ctcactgtaa acattactga aatcctctcc ccattttgta aaagagatct cgggagcttc
     5701 gactaactca taatctgaag aaggaagcca ttctgcatag attttccccc aagtatcttg
     5761 taaagtttgg ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt cttctatagc
     5821 aagcatttct aaatcagcgt acggattttc ttttgtcgta gcaaagccaa tcatgtgcat
     5881 caaactgccc ttttcttcca agcgggctcc atcaaatcca taagaaacgt tgattacttg
     5941 atttggatat aaatctccta acttatgcat ctcttccctt tgagtctccg tgatcgtctg
     6001 cgctaacttt tgtatttctt gattgactcc ttcaaatttg acaggaactt ttttcgccac
     6061 acctgccaca taaaactgtt ctttcttttc gatacgaaat tccatcgatt ttcctcctct
     6121 gacatcaata aaaaatgtca agcgtggaaa agatttttgt atgccgtatc tgctgatttc
     6181 tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa aaaccttcga ctgtttcata
     6241 tccatatcta aacgccgttt ctgtcactct tgatccatct aggagatcgc gattagccaa
     6301 agccaatcta cggtttttga tatagtccat tagcgacatc cctgcaataa acgtaaagat
     6361 gcgttttaat tgatattcag agattcctgt attttcggct attattttat aggaaagtcc
     6421 ttctgtcaaa tgttcttcga tgtaatcgat caattggttc agatctttaa gcatatttat
     6481 cttcctcctt gcttgccttt ttatcatatt atcaaagaaa gtcaattact tgatgaatca
     6541 ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc taaagaaaat tctcaagcag
     6601 cgtttcgtaa cggataattg gtattatctg cctatctatg ttttaccttt agaaaaataa
     6661 attttgggga agacacattc taagtaaaca aagcggtaaa aatatctttt attcccttag
     6721 ttaatccgct tactacctac tgtacaaagc aaattgttgt tcaaactgct gatcagaacg
     6781 ctgcttggat ttttctttgc gatacatcat acctctttta tcaattggaa caattattta
     6841 tttctaacaa aatcacttca tttttgtcag ataaataaaa aacaaaaaaa gttccatctt
     6901 taaaacacgc tattctttta ctttttctca cattctctaa gaaaagaaaa ggagaatgaa
     6961 tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa aatcagctac ttttttctta
     7021 aacgtttctt tatactaaat ataaataagt gtttcatgaa aggagaacta gatgaataat
     7081 tggcttgagt attttcctga aaacgttttg gaacgcggtt acagctatca tctgcacggt
     7141 tttgtccgtc atttgaacta tacaagcaag tacttgtccg caacagtttc tggcacggaa
     7201 gattataagg tagtgatcac ttgggatgaa aagaccaaca tgacttgtga ttgtctatat
     7261 gccatagaag gaaaaaaatg caaacacatg gctgctgtct tattcgctta cgaagaacgt
     7321 ccaataaaaa aatccaacta ctcactttcc gaactctcgt cccttgtttc ctccgctagt
     7381 tcctctcttg tacgggagct tttaacagag attcttatag aacatccgca atttatcgaa
     7441 cgcttcaaag ttaagatgcc atttcatgca ataaattatt cagataaatt aacaactatc
     7501 atccataaat acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc
     7561 atggagatgc gaaagttcat ccaagaagca gttgaatcgc tcattcagca aaatgcctat
     7621 cttcccgcat tcgaattgat caatgaagtg attgctactc tcgaaacatt ctattgggag
     7681 cctgaggatg aacgaaccct tcttctgata gaggactgtt attatttatg gaaagaatta
     7741 ttagcagaag cacctcatgc agaaaaaagg caaatgttct cttggttcgt ctgccaagtc
     7801 gatcatactg acgcctctta ttcaaaacgc tactccataa aaatcctgaa agaagacttt
     7861 cgggaaaaag aattttctaa ccaaaagaaa aaaatagata aaaaaactaa agaaacggta
     7921 aagaaagacg atttcaatga aaaatagcaa gtaaacgata acaaccatcc taaatccaca
     7981 agaaatacag cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa gtaaagaaga
     8041 cttaacgcgt acccaatatt agtcatttga ctattaggta cgcgttttgt tttataagtc
     8101 atgacaaatt tccttcgttg ctgggtagat tttccggcat tgttgataga ttttatgata
     8161 cttttctaca ttttgtaaga gaggttccac tgcatctgta tattggataa aattctgaca
     8221 acaactttca ggtgtgtcga accactctaa tctttaaaga taaatattaa aataacgatt
     8281 aataatatta acagtcttta tttttcacca ccaaaaacct atctttgcat atactaaata
     8341 gacgtatata cacagatagg tttgtcttgt gaaaaataat gattatcctt accaaaaaat
     8401 gtttgttttt ctacatgttc gtacttatca aaaaatatga tccttattga aattactggc
     8461 tatttatatt ccaacgactt cttgtgttaa tttttgttat ctcatagtaa attatgtttc
     8521 attttcactt aatttagttg tcacgtgttc gtcaattcgt aaaaacggta aatatataaa
     8581 cacacttgcg gctataatta atatttgtaa aacagctgct cgccagtctc cggctgttgc
     8641 taaaaatccc gatataactg gtggcgtagt ccaaggaatt tgcactacta catgattaat
     8701 taatcccaca gcagttgcaa aatatgctac tgtcaaagaa aaaatcggtg caagcacaaa
     8761 gggaataatc agactgatat taaatacaat aggcaatcca aaaataatag gttcgcttat
     8821 attgaatata gcgggagtta ccgataattt agcaaagtcc ttataatctt ttctacgact
     8881 gaagataaat attgcaatca ataaagcaat cgtatttccc gatccaccca ttaccgcaaa
     8941 ggtatcttta aaggccatat tgataatatg tggaatttgc tctttgtttg cataagctgc
     9001 catattttct tgcatattct gaatcaaaaa cggatctaac aatgaacctg atattactgc
     9061 ttgatggata ccaaatccaa aaaatagatt agcaatagat gtaattaaca aaaatcctgg
     9121 taaaccagtt gtaattttac ttaatggctc tgtaactatc gtggtaatta gcttatttat
     9181 atccatatta aacaattgaa tgattataaa tgacaataaa gcaaacgagg taatcgtaat
     9241 aatgattggg attaatacat taaaagactt taaaacagcg ggaggaatat ttccggatag
     9301 ctgaatctgt agtttcttat tattagaaag tttaataaat aattctgtag ccgctaatcc
     9361 aacaaatata cctacaaaca ttcctgaagc tcctgtatag gatataggta tcacacccgt
     9421 cacttcaaca gctttatccg cattttcagg agtaaacatc atctgcatag gtgttaatac
     9481 aacaaatgtc gaaatagcaa ctaataccgc tgcaatatga tttgtataat ttctgcttgc
     9541 acacatatga taagatactg ctgctacaac taataatgta gttatgctta atgtaccatt
     9601 ggcaattgaa gtaccaattt gttgtaacgt ggtcaatgta ctagtatcga caatttcccc
     9661 catgaatcct gttggttcta agatcactga attaataagt gtcacaaatc ctgctaacat
     9721 cataaatggc ataaatgtag caaacgcatc tcgcaaagtt cttaaatgga tctgattccc
     9781 caacttagtc gcaactatac caaatttttc aaatacaatg tccttcctgc tacttttgcc
     9841 catatttttt ctcctttatg cttattgtaa tcgattactt tttccaccca aataatacat
     9901 cac
//
LOCUS       DACCXW010000106         9801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCXW010000106 DACCXW010000000
VERSION     DACCXW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.109"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(189..1037)
                     /locus_tag="IVY71_002226"
     CDS             complement(189..1037)
                     /locus_tag="IVY71_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7986265.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1201..2103
                     /gene="gnd"
                     /locus_tag="IVY71_002227"
     CDS             1201..2103
                     /gene="gnd"
                     /locus_tag="IVY71_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7986266.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2105..3106
                     /locus_tag="IVY71_002228"
     CDS             2105..3106
                     /locus_tag="IVY71_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7986267.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3103..3387
                     /locus_tag="IVY71_002229"
     CDS             3103..3387
                     /locus_tag="IVY71_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7986268.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3420..4217
                     /locus_tag="IVY71_002230"
     CDS             3420..4217
                     /locus_tag="IVY71_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7986269.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4231..5064
                     /locus_tag="IVY71_002231"
     CDS             4231..5064
                     /locus_tag="IVY71_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7986270.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5082..5939
                     /locus_tag="IVY71_002232"
     CDS             5082..5939
                     /locus_tag="IVY71_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7986271.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5961..6470
                     /locus_tag="IVY71_002233"
     CDS             5961..6470
                     /locus_tag="IVY71_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7986272.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6507..6908
                     /locus_tag="IVY71_002234"
     CDS             6507..6908
                     /locus_tag="IVY71_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986273.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7020..7373
                     /locus_tag="IVY71_002235"
     CDS             7020..7373
                     /locus_tag="IVY71_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986274.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7407..7850
                     /locus_tag="IVY71_002236"
     CDS             7407..7850
                     /locus_tag="IVY71_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7986275.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7867..8952
                     /gene="uxuA"
                     /locus_tag="IVY71_002237"
     CDS             7867..8952
                     /gene="uxuA"
                     /locus_tag="IVY71_002237"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7986276.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8945..9658
                     /locus_tag="IVY71_002238"
     CDS             8945..9658
                     /locus_tag="IVY71_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7986277.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3256 a         1554 c         2166 g         2825 t
ORIGIN      
        1 tgttatcgca tctaacaagt aactcgaata ataccaatac aaccttctcc aaagatgcga
       61 tgacatacgt tctaacccac cgactattga tgttctgtac cacccttgca gaaccaaaaa
      121 agccactacc aaatatttgg tagtggcttt ttttgtttta tggatttttc gctttttctg
      181 ttatggtgct attctttgat ttcagaaata gtatgtcgaa ctttagcgat agaacgacgt
      241 gattcttttt cgttgtaaaa catcaaaatc acatatagag agtccaaaat gcttagttga
      301 gaaatacggg aagctaaagc ttcagatcgg aattctgttt cttcagaaat agaaataaaa
      361 acgacatcgc caagtttagc aagggaagaa ttagcttggc tagtaataac gattgttttt
      421 gctccagttt ttcttacggt ttcagcaata tggattgttt cttttgattg ccctgtgtga
      481 gaaatcaaaa cagcacagtc tttttccgtc aataaagaag cttccatcaa ttgaatatgg
      541 taatccgtgc tgtgagaagt tgcgataggg gaacgcaaaa atttatgata agcgtcagtt
      601 gctacaatct ctgaaccgcc aacaccgaaa aaatagacac gtttagattc attgataata
      661 gtagccgctt tttttagatc cttttgttgt aacaactttt ttgtgtcatt taaagttgaa
      721 atactagact cgaacacctt ttgcgccata gtgagctcag tatctgtttt ctgaatattt
      781 tcgtggatag aaatagcaga tagatcattt tcttggatca acatggccat tttaaaatct
      841 ttaaatccat tataaccaag tttttttgta aattgaaaaa aagtagagtc agcaatgccc
      901 agttcacttg ctaaattgct aataggacta tgacttactt tgcttggatt ttctaaaata
      961 taatcggcga tagcctgttc ttttgggctg aagtcgttgt acattgtttt gatttgtagt
     1021 cttatggaga cgctcatgag aattcctcat tttcataata agattatcat tttttccgtt
     1081 ctcattaatg ttaaagatac aaggagagta ggaaaaaatc aaacaaaaaa tatttttgta
     1141 aaatctattt acaaaaaaat ttttttgtat aatgagggta taagaaatgg aggcgctatc
     1201 atggaaattg gtgtaattgg tttgggaaaa atgggattga atttagcttt aaacttaaaa
     1261 gagcaccatc acaaagtaca aggactcgac atttcaagta cagctgttag tcaggcaaga
     1321 gaaaatgaca ttcctgctta tcaagagatc tctgaatttc tagctacttt tgagcagaaa
     1381 aaaatcatct ggttaatgct tcctgctgga gagataaccg aaaacatgtt aagaaaactt
     1441 tttccaaaac tcggaccagg agacattatt attgaaggcg gaaactctta ttataaagat
     1501 tcaatccgtc gagcagaaga gtttcagaaa aatgaaatcg gttacttgga ttgtgggact
     1561 tctggaggaa tcgaaggagc acgacatcaa gcctgcttaa tgattggagg agataaagaa
     1621 acttttctat ccgtggaaca attattcaaa gatgtagcaa ttgaaagagg atatctgtat
     1681 gctggtgcat ccggaagcgg acacttcttg aaaatgatcc ataatggaat cgaatatgga
     1741 atgatgcaag caatcggtga aggattccaa cttgtacaaa aaagtgagta tgcttttgac
     1801 ttggaacaag tcgcacgtgt ttggaatcat ggttcggtga ttcgcggctg gctgatggaa
     1861 atcatggaag atcagttaaa cgaacatccc aatttaagta attatcgggg aatcgtttct
     1921 gcatcaggtg aagcaaaatg gacggttgaa acagcgctag aaatggctgt tccagttcca
     1981 gtaattgcaa ctagtttgtt catgaggaat ctttcacagg aagaagatag tttttcagca
     2041 aaagtagttt cagcactaag aaatgggttt ggcggacatg aaatagttga aaagggtgag
     2101 taaaatgcca gattatcata tagcaatcgt taattccagc agtttcggaa gagtctttcc
     2161 acaccacttg gaaagattaa agtcagttgg agacgttcat ttctttactg ttgatcaaga
     2221 gattccagga aaagaactag cggagttact taaaggattc aatatcatta ttgcaagtgt
     2281 tacgccattt tttcgacgag atttttttga aaacaaagat gaactgctat tgatttcccg
     2341 acatggtatt ggttacaaca atattgattt aaaagcagca aaagaacacc agacaatcgt
     2401 atctatcata cctgctttaa ttgaacgaaa tgctgtagca gaaaataata taactaattt
     2461 gttgtccatg cttcgacgca cgacacaagc acaagcaaga gtcaaggacg atcgatggga
     2521 agatcgggcg gagttcgtcg gtaggacgct gtttaacaaa acagttggtg tgattggtgc
     2581 aggaaatacc ggaagcggag taatcgaaat tttgcgtaac ggttttcgct gtgatgttct
     2641 agcttatgat ccttacaaat cagatttata catcgaaagc tttggtgcaa aaaaagtaga
     2701 acttcaagaa ttactggaac aatcagatat catttgttta tgtgcgaatt tgacagaaga
     2761 gaactatcac atgatttctg ataaagaagt cgatcagatg aaagaaaatg tttatctatc
     2821 taatagtgca agaggcgctc tagttgatga agaagcgatg attaatggtc tgaaaagcgg
     2881 aaagatcgca ggatatgcca cagatgtttt agaagaagaa ccgggacgtc aagatcatcc
     2941 gtatttagcg tttgaacaag tagtgattac gccacatata tcggcctaca caagggaatg
     3001 tttagaagca atgggtgaaa aatgtgtcag agatgtcgaa cagatcagtc agcaccttct
     3061 tcctgaacgt tctgtccaga caaagagcaa ctatataaaa ggatgatgaa gaaaatggtg
     3121 aacggtttaa aagtgaaaac aggccctcaa ttttatttat atgaagaagg tggtatttca
     3181 aaagtttctg atctcttgaa atcatacggc gcgaaaagag tactggtcac ccacggaacg
     3241 gtatcatggg aaaaggcgct acctaaatta gtatttttga atgacgaaac gattcaattt
     3301 ttttatcatc gttattctgg agaatgtagt tatgctgaag ccagacgaat agctacaatc
     3361 atcaaaaaaa tgaaatcgat ttcgtagttg gcgttggcgg aggaaaactg accgatctag
     3421 tgggttatag tgccaatctt gcaagaatcg atttcggagt catccctact ttagctagta
     3481 attgtgctcc atggacacct cttgctgtca tgtataagga gaatggacaa gcagaaggga
     3541 aaacagaaca cttttacaga caagctgctt tttttataac agatccccaa ttagtgattg
     3601 attcccctgt tccatatttc attgccgggt tagcagatac actagcaaaa tggtatgaat
     3661 ctgagctcat cttagaacaa gattttttgc aagatgaatc atttttgaag ctagcacaag
     3721 atactgcgaa gatctgcaaa gaagagattt tgaatcattc tgctaaagca atccaagata
     3781 tggcacaaag aaaactgaca tcggagttca aacagctttc agagattatc tttgctgttg
     3841 ctggtttagt tggaggatta ggagataaat acgcaagaaa tgcggcagca catgctatgc
     3901 atgacgctat cagtaaatat attccggaaa gccatcgttt tctacatgga gaaaaagtag
     3961 cgtatgggat tttctatcaa ctggcactag aaggtcgctg ggaaaaaata gacgagttat
     4021 atcctttgta tcgggaatta gaactgccgg tttctctaga acaaatggga ttatttccga
     4081 aagataaaca gataatagat aaaatggctg aatttattga ttcgaaagag aaagtgcatt
     4141 tgattcctat agaggtaaca cagaaatcac tgatacagtc aatgatggaa ctggaaggtt
     4201 atatcagaaa gatctgaagg aggaggacac atggaaacat taagtgtagt acaaagtttg
     4261 ttgattgctt tatgggtagc cgcggttatg tcccgctggt taggtggagg agccacgttg
     4321 accttacgtt tttctccttt gatgactgga ttagtcgtcg ggttgatcat gggggaagtt
     4381 gcacaagcga tgattgttac agccgctttg caaatgattt atatgggggt tttttctcca
     4441 ggaggatcaa tgccagctga accatctatt gcagcagcta tcgcagtacc agtcgcatta
     4501 ttaggggatc tgtcgccaga agcagcgatt gccgttgcgg tgccagtcgg attgctcgga
     4561 agttacttgt atcagtttcg ttttttcatc aatacttttt taggtaaata tacagacaaa
     4621 gctgtagaag atttgaatac aaaagctatg gcccgatcaa ttattcttta tccaacggtt
     4681 gcctcatttt tattattcgt tccgcttgtt ttcattgctt tatatttagg ggcgccattg
     4741 atcgctgatg taatcaaagc tttagaggga acagtcgtta tccacattct tgaagtagta
     4801 ggtggagggt tagcggcaat cggtatcgcg acaactgtat acgttatcgg tcgaaaagat
     4861 tatctggtct ttttcttcct tgcttatttt atgagtgttg ttttgaaatc tttagatatt
     4921 acgatggtta cttatgccat ttttgggatt attatcgctt tgatctttgt ccagtcgcaa
     4981 aaagggaaac aagtacagcc tgccacaagc aataacaatt cagcatcttt tgatgacgaa
     5041 gatgactatg atgacggatt ttaatttagg aggaatggga aatggctgaa ataatggata
     5101 atcaaacagg tgctcctgaa gaaatcacga aaaaagatgt aacaaaagcc tatctgagat
     5161 ggcattttgc aaacgaaatt cctcactctt ttgaacgtta cttggcgcca tcactactat
     5221 acggaatgat gccaatcttg aaaaaacttt ataaagatga agactcttta aaagcagcat
     5281 ataaacggca gttactgttc ttcaacactc aattaagctg gggtggcggt gtgattactg
     5341 gcctaatgtc ttctatggaa cagcaaagag cagaagaaga gtatgaagaa aaagagatct
     5401 tgatgcagga tgacttgatg tataacacga aagcgggatt gatgggggca ctagcaggaa
     5461 tcggagatgc aatcgattca ggaaccgtcc aatatatttt tatcgcaatc gcggtacctt
     5521 gggcacaaca gggaagtcct ttaggcgcac tttttccttt tatctgcttt gggttgtatc
     5581 aggttttact aggggtattt ttcgcaagac aagctttcac tatgggccgt aatgctacag
     5641 gcctaatgca aaacactggt gttcagacag cgattgaaat gctatcagtt ttagggttgt
     5701 ttatgatggg ggtgcttgcg ggaaactatg taaaagtaga atcttcattg aaatttgcga
     5761 tatccggacg tgagtttgtt atccaggaaa ttcttgatca aatcgtgcca gggatattac
     5821 ctttagctgt cgtaatgggt gtgtacttgt tctatacgaa aaaaggattg aaagtcacac
     5881 aagcattatt atggctgact ggaatattaa ttgtgctagc aggaatcggt attttataaa
     5941 aagaagggaa cgtgaataag atgggtgtag tcaatttagc gcgtgtggat gagcgtttga
     6001 tccatgggca agtaatggta acgttatctc aaaaaagtgg agtgaattcc atttttgttg
     6061 tagacgaagt tgtagcaaaa gataaattta tgagagatct ttacaaaaat gcaggaagcc
     6121 gtacaggaca aaaaacaatt gtcattacac cagagaaagc taaattctat tgggatgaat
     6181 atcaatttaa agaatacaat tgtattttga tcgcaaaaac agtccaaacg atttacgact
     6241 tagtaaaaca cggaatcccc atggaagaat tgaatattgg agggattgct caaaaaaatc
     6301 ctgatacaga tcttttagtg acgaaatctg tttatttaaa taaatcagat gctgaaaaat
     6361 tgaaagaaat gaatgaacaa tatggcgtaa agaatattta ttttcaagcc actccatctg
     6421 cacctaaaac aggattaaaa gatgtattga acaagtttga tttgtcataa acaaatgctt
     6481 agaaaaaaga taaagagggc gaaaaaatga agaagaacga gctgtttcgt gattgggaat
     6541 ttcgttatcg ctatatttac cgtaagcgcc gtacaaaaaa gagtaaacag cgttttcttt
     6601 cagcattagt atcagatatt tacagtatga gaacagatgt gacagtcatt gcttacgata
     6661 cgctagccta ccgatcaaaa aatatttatg ttggagatat cgaaaaagca gagaaagtta
     6721 tctgtactta ttacgataca cccgttcatg cattaggaag ttatttcatg tttgattgga
     6781 aagaccaaag gaaaaaaaca atctactcta tattattgtc cttcattttg ttgttcagtt
     6841 taggttggtg gggaatgatg atatataaca aaaatcctca tcacgtattc gatctgctat
     6901 ccgtataaac aggtatcact gtactagcat ttgggagcta ctttttctta ttgggaaaag
     6961 cagctcgcgg atggcctagc agacaaactt ttattcgaaa tacttcatcc atattaacga
     7021 tgctagaaat gatccgtacg attgatgatc cgagtgtagc gtatgcgttt gttgatgaag
     7081 gctgttatgg agaaaagggc ttagacagcg tgagatcggg tatgaaaaaa gaggctattc
     7141 ttttttatct agactctgtt ggggcagata caccgctaca gttttcggga aattattttt
     7201 caaataaaga acagtggctg aaacaagttg ataagttgaa agaaaaaaac gtcaattata
     7261 tttttagtgc aagaaaaaag caagcgcagt tcttctattt gaccaaaaca gatttacgag
     7321 ggaaaacatt taattggcaa aatgccaatc agattattgc tctttttcga taagtaaaaa
     7381 gtgcaaaaca aggagtgaga agactaatgt tggaaatcat gatcgttaca catggtaagt
     7441 tgagcgaagg actgaaagat gcggcggaag taattgtcgg gacatcgagc catattgaaa
     7501 caatgtgctt agagtctggg gaggacatcc aaacattcgg agagaaggtc aaagaaaaat
     7561 taactatgat caacacagca gaacccatgc tagtatttgt tgatctaatt agcgcaagtc
     7621 cgtacaatca agcgctcttg gcagtcaatg aactgactga agaaaagaaa gcagccactt
     7681 atgtcatagg gggcgtgaat ctgccaatgt tgttagaagc aatcaatcat cagatgcttc
     7741 acacaccgat agaagaagcg gtggaacaaa ttgtcaaaca aggaaaagat aatttaactg
     7801 tttggcatgc ttcagcggaa gaaaaaaaat tagatgagga cgatttttag aaggaaggct
     7861 gaaaagatga agtggggatt tagatggtat ggtgaaaaag gggattcgat cccattaaaa
     7921 tatgttcgcc aaataccggg aataaatggg ataattggaa ctttgctgac taaaatgcct
     7981 ggagatgttt gggagatttc agaaattgag gagttgaaag catctgttga gagccaagga
     8041 ctggaactgc ttggaattga aagtgtagcg atccacgatg caatcaaggc agggacagat
     8101 gaacgagatt actatattga taactatata cgaacaatca aaaacttgtc tcgatgtggg
     8161 gttcgattga tttgttatag cttcaaacca atttttggat gggcgaaaac tgatttgttt
     8221 aatgaaaaca acgatggaag ctattctctt ttatacgatc agaaaatcgt cgatcagatg
     8281 gagccaggag aaatgtatca attgatacac agccagtcaa agggattcaa attgccgggt
     8341 tgggaagaag aacggttgaa aaaattcgct cagctggaaa aactgtatga aggaatgacc
     8401 gaagaagaat tatttgaaaa tctgacttat tttttagaaa aagtgattcc agtgtgcgaa
     8461 aaagcagatg taaaaatggc tatccatcca gatgatccgc catgggaact ttttggtttt
     8521 cctagagtga cgaaaaatct tggcgacttg aagaaaattc taaatgcagt agattctcca
     8581 tataatggac taacgctgtg cacaggatct ttaggggcaa atccggaaaa cgatttagtt
     8641 gaaatcatcc atgaggtagg ttcgcgcatc cattttgtcc acttcagaaa cgtggagttt
     8701 ttaggagaaa gaaagttcaa agaaacggca catccctcta cagaaggctc ccttgatatg
     8761 tatcatatta tgaaagcgtt ggtgcaagaa gggttcgacg gtcctatacg ccctgatcat
     8821 ggtagaaccg tgtggggtga agtggctatg cctggatatg gcctgtatga tcgtgcaatg
     8881 ggactcactt acatgcaagg tctatatgag gcgataagta aggaaaaacc actgggagag
     8941 cattatgaat aaaatcaagc tcttcatctt tgatatggat ggccttttat tagaaactgg
     9001 acgtttagct tatcgtgctt atgtaaaatc agcacaaaag tatgattatg aaatgcgtaa
     9061 ggaagtctat tatttactaa ctggccaaac agaaatggcg ataagagaac aaatgggcat
     9121 tttgtacgga gaagatgttc catatatcaa atggcgagaa gcaatcaatc aatataaaga
     9181 aaaaatcgtg aaagaagata agagagtata tacgaaaaaa ggagcagaag aaattttatc
     9241 ttttgcaaaa gaaagaggaa tccatacaat cgtggcttcg tctaatacac gggtaaaagt
     9301 agaaatgtat ttaaaaatgg aaaatctata tgccttgttt gatcaaatca tttcagggga
     9361 tgatgtaaaa aaaggaaagc cggaaccaga gatctttttg aaagcttgtt caaaaatgaa
     9421 tattcctcca agtgaagcac ttgtatttga agactcgatt gcagggatcg aagcagcaag
     9481 gcgagcgggc attttgtctt ttctcatcaa agatcatttg atagggttgc ccgatcataa
     9541 tgggaagcat aagttgaaag tagatgtaag tttatatgaa tccaataaag cagactatga
     9601 attttatgac ctgcatcaag caaaacgatt tttagaaagt aaagggttgt atctctaata
     9661 aagaggacta ttttctagtc ctctttattt attgtcctgc acgactgagg gaacagatga
     9721 aaaagaagta attttttatt ttgaagtatc taccttattt gtagtgaaca atttagaaca
     9781 aacacaaaac gcatctactg t
//
LOCUS       DACCXW010000107         9738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCXW010000107 DACCXW010000000
VERSION     DACCXW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.110"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            59..3337
                     /locus_tag="IVY71_002239"
     CDS             59..3337
                     /locus_tag="IVY71_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7986278.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3878..5785)
                     /locus_tag="IVY71_002240"
     CDS             complement(3878..5785)
                     /locus_tag="IVY71_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7986279.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5915..6790
                     /locus_tag="IVY71_002241"
     CDS             5915..6790
                     /locus_tag="IVY71_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7986280.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6870..7844
                     /locus_tag="IVY71_002242"
     CDS             6870..7844
                     /locus_tag="IVY71_002242"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7986281.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8033..9169
                     /locus_tag="IVY71_002243"
     CDS             8033..9169
                     /locus_tag="IVY71_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7986282.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9306..9650
                     /locus_tag="IVY71_002244"
     CDS             9306..9650
                     /locus_tag="IVY71_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7986283.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3173 a         1800 c         2060 g         2705 t
ORIGIN      
        1 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
       61 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      121 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      181 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      241 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      301 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      361 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      421 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      481 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      541 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      601 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      661 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      721 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      781 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      841 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
      901 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
      961 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1021 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1081 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1141 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1201 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1261 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1321 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1381 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1441 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1501 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1561 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1621 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1681 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1741 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1801 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1861 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     1921 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     1981 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2041 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2101 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2161 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2221 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2281 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2341 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2401 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2461 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2521 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2581 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2641 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2701 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2761 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2821 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     2881 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     2941 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3001 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3061 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3121 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3181 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3241 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3301 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3361 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3421 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3481 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc
     3541 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3601 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3661 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3721 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3781 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3841 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     3901 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     3961 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4021 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4081 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4141 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4201 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4261 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4321 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4381 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4441 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4501 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4561 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4621 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4681 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4741 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4801 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4861 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     4921 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     4981 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5041 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5101 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5161 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5221 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5281 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5341 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5401 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5461 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5521 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5581 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5641 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5701 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5761 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5821 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     5881 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     5941 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6001 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6061 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6121 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6181 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6241 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6301 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6361 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6421 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6481 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6541 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6601 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6661 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6721 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6781 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6841 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     6901 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     6961 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7021 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7081 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7141 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7201 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7261 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7321 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7381 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7441 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7501 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7561 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7621 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7681 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7741 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7801 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7861 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     7921 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     7981 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8041 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8101 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8161 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8221 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8281 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8341 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8401 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8461 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8521 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8581 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8641 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8701 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8761 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8821 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     8881 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     8941 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9001 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9061 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9121 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9181 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9241 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9301 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9361 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9421 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9481 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9541 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9601 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9661 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9721 gcaaattagt tgtctttt
//
LOCUS       DACCXW010000108         9719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCXW010000108 DACCXW010000000
VERSION     DACCXW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.111"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            320..1369
                     /locus_tag="IVY71_002245"
     CDS             320..1369
                     /locus_tag="IVY71_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7986284.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1366..2247
                     /locus_tag="IVY71_002246"
     CDS             1366..2247
                     /locus_tag="IVY71_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7986285.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2250..3020
                     /locus_tag="IVY71_002247"
     CDS             2250..3020
                     /locus_tag="IVY71_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7986286.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3299..4153
                     /gene="aroE"
                     /locus_tag="IVY71_002248"
     CDS             3299..4153
                     /gene="aroE"
                     /locus_tag="IVY71_002248"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7986287.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4331..5494
                     /locus_tag="IVY71_002249"
     CDS             4331..5494
                     /locus_tag="IVY71_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7986288.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5611..6021
                     /locus_tag="IVY71_002250"
     CDS             5611..6021
                     /locus_tag="IVY71_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7986289.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6195..8423
                     /gene="topB"
                     /locus_tag="IVY71_002251"
     CDS             6195..8423
                     /gene="topB"
                     /locus_tag="IVY71_002251"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7986290.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8561..8878
                     /locus_tag="IVY71_002252"
     CDS             8561..8878
                     /locus_tag="IVY71_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986291.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9038..9274
                     /locus_tag="IVY71_002253"
     CDS             9038..9274
                     /locus_tag="IVY71_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7986292.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            9271..9669
                     /locus_tag="IVY71_002254"
     CDS             9271..9669
                     /locus_tag="IVY71_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7986293.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3376 a         1650 c         2066 g         2627 t
ORIGIN      
        1 gacttaaatc aattcttaag gaaagggtga cctgtaatga acttatcaaa acgtatttca
       61 gcagaaaaca aaaaaaattg gcgcagctgg cgtagataag ttgtattaat ggtgagactt
      121 tcccatagat acaacttttt agagtgcatt tctaaagctt gtatctatgc tagacatgaa
      181 cattgactgc cgcatagatt agaatctatg cggttttttg tgtaaagaca atgaccgctt
      241 tggccattgt ctttttttgt agactggata cttgcctgat tataataaat aaaaagattg
      301 actttgcagg aggaaaaaaa tgaaaaacaa acgattaata acagttgtag taattatttt
      361 agtttattta gcaggtgcat ttatttatgg aaaagcctca ggcgaaaaag aaagtacttc
      421 gacgaaccag acaaaaaaaa caacagttgt tggggtattg caatacgtga gccatccggc
      481 attagatgag atttatcgag gaatcaaaga tggtctgaaa gaagaaggat taaaagaagg
      541 agaaaatctt gagatcaaat ttcaaaatgg acaagccgat cagagcaagc tagccacaat
      601 gagtcaacag ctagtacaat ctgatccaga tgccttgatt ggaatcgcaa cacctgccgc
      661 tcaatcatta gcaaacacga cttcatcgat cccagttgtg ttaggtgctg taacggatcc
      721 tgtgggggct ggattagtcg cttctttaga gaaacctggt ggtaatgtaa caggtgtttc
      781 tgatcagccg cctgttactt cacaaatcaa gcttgcttct gatttgttgc caaatgcaaa
      841 aaaagtcgga atcttgtatg cttcttcaga ggataattca aaataccaag tagcacaagc
      901 agaagaagct gcaaaaaaag aagggttaga atcagtaaaa tatgcggtgc cttccactaa
      961 tgagattgca caaacggttc aagtgatgag ccgacaagtc gattttatct atgttccatt
     1021 agataatacc attgccaatg cgatgcaaac agtggtaaaa gaagcaaaca aagcaaatat
     1081 ccccgtcatt ccatctgtgg atacaatggt ggaacaaggt ggactagcaa caatcgggat
     1141 caatcaatat caattagggc ttcaaagcgg taagatggcg gcagaacttg cgagtggaaa
     1201 agaaaaaccg gaaacgacgc ctgtttatac atttgatcaa ggggatacag tgatcaatca
     1261 atctcaagct gatcgcttag gaatcattgt tccccaatcg atgaaggaaa aagccaaaat
     1321 cattacagat gactcgcagc aagaaacatc taaggaggat gacaaatgat cgtatcagct
     1381 atttcacaag gcatgctttg ggcaatatta ggattaggga ttttcatgac ttatcggatc
     1441 ttagactttc ctgatatgac gacagaaggg tccttcccat taggtgctgc tgtctgtgtg
     1501 acagctatta ctcatggggt agcgccgatt gtagctacat tattaggcgt aggtgctggc
     1561 atgatagcag gactcgttac ggggttactt tatacaaaag gaaagatacc ggttatttta
     1621 gcagggattt tagtgatgtc aggattgaac tcagttattt tgtttgtgat gcgaacacca
     1681 aatctttctt tattgaatca tccgattcta caagacagct tccatcgctt tgggttacct
     1741 gattactttg acacagtcct tcttggcttc atctctttgg gcatcttgat tgctgtactg
     1801 ctattcttct tcaatacaga tttgggtcaa ggctatattg cgacaggaga caacgaaacg
     1861 atggctcggt cgttagggat aaaaaccgat cgtatgaaaa ttttaggttt aactttgtcg
     1921 aacggtgtca ttgcattatc cggtgcgctg attgcccaga acgacggata tgcagatgta
     1981 aataaaggga ttggcgtaat cgtcatcggt cttgcttcca tcatcatcgg tgaagtgatc
     2041 ttccaagaat tgacgctagc agaaagattg attgctattg ttgtcggcag tatcatttat
     2101 cagctattga ttttagtagt aatcaaacta gggtttgaca cgacttatct gaagttgttc
     2161 tccgctatca tcttagctat ttgtctgatg atcccgcaat tgaagaaagc actgaactta
     2221 aaaacgggtt ttgagaagga ggcatagtaa tgactgatat attgacaatc aaaaatggca
     2281 gaaaagtcgt aaacaatgga atgaatgaag agaaagtact attggatgaa attaacctta
     2341 caatcagaga aggggacttc atcactgtcc ttggcgggaa tggtgctgga aaaagcacac
     2401 tgttcaatac gatcgctggt acgcttcaac tcagtcaagg atcgatctct tttaaagatc
     2461 agctgatcac aaaagattca gaagaaaaaa gagcacgatt cctttctcga gttttccaag
     2521 accccaagat gggaacggct cctcggatga cagtagcaga aaatttgcta cttgcacaaa
     2581 aaagaggtca aaaacggaaa ttccgtttac gtaacttgaa aaatcaaaaa gaagattttt
     2641 tcagactatg tcaagaagtc ggtaatggcc tagagcaaca cttggatact ccagcgggtg
     2701 aattatctgg tggtcaacgc caagcattaa gcttgctgat ggcgacgatc acagctcctg
     2761 agttactatt attagatgaa catactgcag ctttagatcc taaaacagct aaagcattaa
     2821 tgcgattaac aaatcagcgg atcaatgaac aaaaattgac ttgtctcatg atcacgcatc
     2881 gaatggaaga cgccttgaaa tacggaaatc gtctgatcgt actccaaaaa gggaaaatcg
     2941 ttcaagatat ttctgaaaaa gaaaagcaac atttgacaat gggcgacctt ttgcagttct
     3001 ttgaagaagt agtggattga tttcgacaga aaaattcctt tcagtagaag gacttgaaaa
     3061 ctcattgaca tcttcctttg attcatccta tacttaaaac aataaacaat taaacatatt
     3121 tgggtgtttt gtaggatcac gtagtcaatg attttaacaa ttaaatagaa tattctttgc
     3181 tctgacaagt aaacgatgag aaaagaattt gctactttgc cacctagacg tttcgtactt
     3241 tttcgtatca aacgctaagt ggcttttttt attgaagtaa gaacaaagga ggaataagat
     3301 gatttctgga caaaccaaat tagctgggct ctttgctgat ccggcttctc acagtttatc
     3361 cccattgatg cacaatactg cttttgaagc aaacaacatc gatgcggtgt atttagcttt
     3421 ccaagtaaat ccaatgaatt tgaagcaagc agtcgaaagt attcgaactt ttgatatgtt
     3481 aggtgtaaat ctttcgatgc cgaataaaac agcggttatt ccttatctgg atgaggtaag
     3541 ccaggaagcg caattgatcg gagcagtgaa tacgatcgtg cacgaaaagg gtcgtttgat
     3601 tggctataat accgatggtg cgggcttcat gcaatccgtg caagaagctg ggatttcgat
     3661 caaaaagaaa aaaatgactc tactgggagc tggaggtgca gcaaaggcag tcactgttca
     3721 agctgcgtta gacggcgctg aggaaatcgt agtttataaa cgaaacaacc aaacattcgc
     3781 tcaagtaaaa gaaacattcg aacaactcag ctatcgaact tcttgtaaga taaaagttcg
     3841 tgactttgcc gatacgcatc aattaagaaa agacttgaaa gaaagctgtc ttctagtcaa
     3901 tgcaacagat atcgggatgg gaaagaaaat caatcagtca ccgctcttag atataagcat
     3961 gatgcatgag aatctagcgg tttttgattt gatttatgca cctagagaaa cacgtttatt
     4021 aaaagaagca aaaaaagtag gagcaaaagg atacaatgga cttggcatgt tgatctacca
     4081 aggagcaatc gcgtttgaat tatggacaaa ggaaaagatg ccgatccatg tgattgaaaa
     4141 gctgtttgat taaaaaagga taatagataa agatctttta ttctcgcata aacggtctca
     4201 agaaaagtaa tcctttatgt atcgaagcat ttatttcgtt attttttatt aaattatcaa
     4261 aaaatgatag gcttttgtcg attggttatc gtatccttta tttatcagta agaaacgagg
     4321 aggaaccaca atgagtcgga tggatcgcta taaggacatc cataagaagg cgaaacctct
     4381 gaagcgggat acggggagta tttttgctag aagagaacga aaacgaacgc gtatggaaaa
     4441 tgaggaagat gctacccgta tttatgaaag agagcagcat tcatttgaac atttgaacca
     4501 atacgagcaa cagatataca gagatgcacc aagagcagaa gaacaacaaa aaaataaaaa
     4561 atttttcaat cggcaaaaga agaacaaacc gttaaaacct cgaagaaagc gatcgtggaa
     4621 aaaaatcatt ctatcgattt tgcttgtttt gatcctttat tcaggtatct cattttttat
     4681 tggtaaatat gtagctgaac atgacacaag cttagaggca gcgacttcag aaacctttca
     4741 tgggcaaaaa gcgacgaatg gtgcgcataa tattttgatc cttggaagtg atacacgcgg
     4801 agaagatgcc gggcgagccg atacgatcat ggtcttacag ttggatggtc cttcccataa
     4861 gccgaaactg atttctttta tgcgtgacag cttcgttacg atcccagggg taggacaaaa
     4921 taagatcaat tcagcttatg catacggagg agccgatcta gtgcgtcaaa ctttagtaga
     4981 aaattttgga atcgattgtc aatactatgc gaaagtcgat ttcaaatcgt ttgaaaaagt
     5041 gatcgatgcc ttattcatga acggagtgaa gatcgatgca gaaaaagatc tgaatctgga
     5101 tggtgtagat attaaaaaag gggtccagaa gatggatgga catgttcttt tgcagtacgc
     5161 acgtttccga atggatgaac aaggagactt tggccgtgtc agacgccaac agcaagtaat
     5221 gaacgctatt ttcagccaat tgaagaatcc attgaatctg atccgtgcac cttatgcagc
     5281 tggtaaagct ttaggatata catccacaga tgtcactgct tggttcttga cgaaaaacct
     5341 cttttcgatt gcacgaggag ctggtggcat tgatcgaata agtgtgccag tagacggttc
     5401 gtggaattat gggcaaagtg attatgctgg cagtattctt gaaattgata atcaaatgaa
     5461 tcaatctgct attacagatt ttttgaataa atgatcataa aaaagatcaa gaagaagctg
     5521 cgcaaggctt ttgtcatgaa tcaatcaaac gattcactcg taaaaaacgc tttcttatgg
     5581 tagatttaac caagagagga gctgtttaca ttggatatct atctgaactt caaaaatgag
     5641 tcgcgtgaag ccatcgcttt ttatgaaaaa gtttttcaga ctgaagcatc aggtatcacc
     5701 acatacggtg aaatgcctgg aatagaacaa atgaacttgc cagaagaagc aaaaaatcta
     5761 atcacgaatg cacggctgaa catttatgat acattagtta tgtttgcaga tacgccagaa
     5821 ggaattggtc ctgaattagt tgtgggaaat aatgtttctt tagtcatcca gacaagaaat
     5881 gaagaagaaa cgaagcgcct atttgaaagc ttagctgagg gtggtaagat tgagatgcca
     5941 ctggatgctg ttccgtgggc tcagttgtat gggatggtaa ctgatcgatt tggcatcaac
     6001 tggcagatta accaagagtg acagctaaag atgaatattt tctggaaaca tcaagcagca
     6061 aacggatcat cgtttgtttg cttgatgttt ttgtgtctat ttcgactata cggtatcttt
     6121 tttttattgc cagatgcgaa aatatgttcc ataatagaga agtccgcaaa aaattaagta
     6181 acggaggtca gaagatgaag acggtgatac tagctgaaaa accttcacaa gcaaaagcct
     6241 atgccgaaac cttccaacag caaaagcggc atgaagggta ttatgaaatc agcgatccat
     6301 tgtttgctgg tgaagtaacc atcacttatg gttttggaca tttggtagat atggttgctc
     6361 caggagctta tgaagagcgt tgggcaaaat ggtctttaga aaatttgcct attttcccag
     6421 aaaccttccg ttacgaagtt ccaatggaca aacgtgcaca attttcgatc gtaaaacgag
     6481 aattgcagtc agccgatacc atcattattg caacagatgg tgatcgagaa ggtgaagcca
     6541 tcgcgtggtc gattattcga caagcaaacg cattttctaa ggaaaagaac tatttacgtt
     6601 tatggatcaa ttctcttgaa aaagaagcaa tctatgaagg ttttaagaat ctccaacctg
     6661 gaaagaacta ttttccaaaa tataaagaag cgcaagcaag gcagaacgct gattggctga
     6721 ttggaatgaa tggaagtcca ttatatagct tgctacttca acgaaaaggg attgatggca
     6781 gtttttctct tggtcgtgta caaacaccga cactctatat gatttatcag ctgcaagaag
     6841 aaatcaaaca cttcaaaaaa gaaacgtact ttgaaggaga aggtttaatt acgacaaaca
     6901 aagggcagtt ttctggaaaa atcgtaccaa aaaaagcatt taaaacccaa gaagagttaa
     6961 cgaaggaaat agaaaaactt ggagcgcatt taggtaagca aaacggacgg attcttgaag
     7021 ttacgaaaaa ggaaaagagg ttgcatagtc cacgtttatt ttcgctatct agtttgcaga
     7081 caaagatgaa tcagctaatg aaagcaagtg ccaaagctac acttgatgct gctcaaggat
     7141 tgtacgaaag caagtttcta agctatccaa gaacggattc cttttacatt acagaaaatg
     7201 agcatcagta tctggttaaa catctgcaaa attataaaaa atttttagga atagaagaag
     7261 tacagacaac tgagatcaaa gcaaagaaac gatatgtcga tgcgaaaaaa gttcaagaac
     7321 atcatgcaat tattccgaca aagacgattc cgactcctgc acgctttgcg aagctttcca
     7381 aattacagca agcaatctat ttgcaagttt tgcgcactac tgtggctatg tttgctgcag
     7441 attatatcta cgaagagacg accgtaatga cgggcgtaca gcaattaaag ctaaaaagca
     7501 tcggaaagat cccattacag caaggttggc aggaaatctt acgcatcaag tcaaaaaaga
     7561 aagaagctca gactcttcct cctatcgtag aaaatgaaga agcagaagta gaactgaaaa
     7621 gtatggagaa agaaacacaa cctccaaaac cctataccga aggaacgttg atcacagcaa
     7681 tgaaaactgc gggaaaaacg ctggataatg atgaagcgca agaaattctc aaagaagtag
     7741 aaggaatagg gacagaagct acacgagcca atatcattga aaatcttaag cagaagcatt
     7801 acatcgaagt caataaaaac gaaataactg ttacgccaaa aggaatcact ctttgtaagg
     7861 cagtagccaa tgaaccatta ctgacgagcg cagaaatgac tgcgcgctgg gaagggtact
     7921 tgagaaaaat tggtaaacaa gaggggacac catctgtttt tttagagaat atcaaaaaat
     7981 tcatcctaca tttattggca gaagttccta atcaaatcga caaggtagac ttttcagatg
     8041 agattagcgg gacacgtcag ataaaagcga tggagaagaa aaacgatcaa ttaggaagat
     8101 gtccaaaatg taaaaaggga atagtcatgc tttatcctaa aatcgctaca tgcttgaatc
     8161 cggaatgcga cttcaaattg tggccaacag tagctaaaaa gaagctgact aaaacgaatc
     8221 tcagagattt gctttcaaaa ggaaagacaa gtaaagttgt caaaggtttt actggtaaaa
     8281 aagggaaatt cgatgcagtt ttagtattaa aagaagatat gacaatcggt ttttcttttc
     8341 catggatcga aaacagtgga acaaaagcag ttgatggaaa caaagaaaca tcaagaaaaa
     8401 ataatgaaaa cgaagtagag taaatgaaaa caatgaaata gcggaatatt tattccgaaa
     8461 agtgagaata taaataccaa taaaactaaa aaatatcagt gaatcaagca aaaacgttgg
     8521 ggtaggctga ttttatatag atagcaagga gtttttttat ttgaaaaaag tgcattcatt
     8581 tttaaattat cttatgggag caatcacgat tgtggctgtg taccaagcat ttctaaagta
     8641 tcctgattac gggacgaagt tgttgtcatt gacgcacaat caaacagcat attcaccaac
     8701 gttgatttta tcgactttga ctgcaggagc taaaagcgct acttattttt ttcataaaac
     8761 gacaagcatg ctaaagcgaa agaaacagcg aaaacaattg ttggtggtgg tagcggctat
     8821 gatcattatt tattttctac cagttattac acaagtattt catcaatatt ttggttaaga
     8881 atacgagagt ttgtgacaaa agtcatgtca caaacttttt tctttgtcat tttttcgtaa
     8941 gaaaaagcaa aataaaagta tatacattga ttaaaagtat atacttggat ataatgtact
     9001 tactatagaa tgagaatggt atagagggtg gcgaaatatg aaaaaaatga aaaagtataa
     9061 aattagaaaa tctggcaata gcgatgtaac aactatccca ccagaagtaa aagaattcat
     9121 gggtgtgcag acaggtgacg caatcagtta tgtttttcaa tctgatggtt ctgtacgaat
     9181 gatcaaagca caagaagaac ctgatattga ttcattagtt gattcgatca tgaatcaata
     9241 tgaagacgcg ttaaaagatt tggttgattt atgaggtatc tttcgactaa agaaattatc
     9301 aaaatcaacg cgaaagttat tttacgtcac tcgccaggcg aaatgatcgg tgtaaaagat
     9361 gcaaatgcat tagatatggc cgtcaaacag ccttcacaag cagtattcaa tcaagaactc
     9421 tatcctgaaa tctatgaaaa agcggcgatt ttagcaatta atttagctaa aaaacatcct
     9481 tttcataatg gcaataaacg aactgctctt gttgccatgt tgttgtttct tcaattgaat
     9541 aattgtccag ttgctttttc tcgtcaagaa gctgttgatt ttatcataat gattaccact
     9601 agttcattgg atttcgattc actaaagtcc aagataacca gttatttacg acaaaccgca
     9661 ataaaatgaa cgaattaaaa actttatttc tcaattagat cagcatgtca ttaaggaat
//
LOCUS       DACCXW010000109         9485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCXW010000109 DACCXW010000000
VERSION     DACCXW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.112"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            29..3721
                     /gene="nifJ"
                     /locus_tag="IVY71_002255"
     CDS             29..3721
                     /gene="nifJ"
                     /locus_tag="IVY71_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7986294.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            3910..4797
                     /locus_tag="IVY71_002256"
     CDS             3910..4797
                     /locus_tag="IVY71_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7986295.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            4794..5963
                     /locus_tag="IVY71_002257"
     CDS             4794..5963
                     /locus_tag="IVY71_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986296.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            5987..7342
                     /locus_tag="IVY71_002258"
     CDS             5987..7342
                     /locus_tag="IVY71_002258"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7986297.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(7414..9219)
                     /gene="glmS"
                     /locus_tag="IVY71_002259"
     CDS             complement(7414..9219)
                     /gene="glmS"
                     /locus_tag="IVY71_002259"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7986298.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         2975 a         1811 c         2117 g         2582 t
ORIGIN      
        1 gagagaatat gaacaaggat gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac
       61 ggcagccgca tatatttctt atgcatttac cgagattgct gcaatatatc caataacacc
      121 cagttcgacc atggctgaat tagtagatca atggtcagca gcaggaaaag aaaacgtatt
      181 cggtcaacca gtaaaagtag tagaaatgca atccgaagca ggagcagctg gtgtggtcca
      241 tggatcattg aaaactggtg cactgacaac tacatatacc gcttcacaag ggctgctttt
      301 gatgataccg aatatgtaca agattgctgg ggaactactg ccaagtgttt tccacgttgc
      361 tagtcgggca gtgacgacaa atgcgttgaa tattttcgga gatcacggag acgtgatggc
      421 tgcaagacaa acaggatttg ctatgttagc tgaaagcagt gtacaagaag tgatggattt
      481 agcacctgtt gcccatcttg cttctattga aggagaagtc ccttttttga atttctttga
      541 tggattccgt acgagtcacg agattcaaaa aatcgaagtt ttggaatatg aagaattagc
      601 tcctctgatc aatcaggaga aattagcttc atttcgtaaa cgagggatga atcccaatca
      661 tccgactgtt agtggtacga accagaatcc agacatccat tttcaacaac gggaaacgat
      721 caacccctat tatgataaat tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg
      781 attacgagga acttcttatg acttggtgac ttattacgga gcagacgatg cggaagaagt
      841 cattgtttct atgggatcag ttgcacagac gattgaacaa acgattgatt acttgaatgc
      901 aaacggacgg aaaacaggtt ttttgaatat ccatctttat cgtccttttc cagtggaaac
      961 attcgtgcaa aagttgcctc atacagtaaa aacaattgct gttcttgatc gaagtaaaga
     1021 accaggggca ggtggagaac ctctcttatt agacgttcaa agtgcgttat atgattctga
     1081 acttcgcccg gctgttattg gtggtcgcta cggattgggg tctaaagatg tgactcccga
     1141 ccaaatctca gcagtttttg atgaattgaa aaaagaccca agcatacgca aaaaacggtt
     1201 taccataggg atcgtggatg atgtgactta tcaatctttg gaaccaaagg aaagtctgga
     1261 tttgacagaa ccgcaaacat tccaggccaa gttttgggga tttggatctg acggaacagt
     1321 tggagcaaat aaatcagcta tcaagattat tggagatcac accgataaat atgcgcaagg
     1381 ctatttttat tatgattcga aaaaatctgg cggtctgact gtctctcatc ttcgttttgg
     1441 agatacgccg atacgttcag cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac
     1501 accagcttac ctgcatagct atgacttggt caaaggattg aagccaggtg gcattttttt
     1561 attgaatact ctttggagcg acgaacaatt agaaacgcat ctgcccttaa aattaaaacg
     1621 ttacttggct gaaaataaca ttagattcta tacgatcaat gccatgagat tggcccaaga
     1681 agtcggtttg ggacgccgga tcaatacggc aatggaaacg gcttttttta aattagctga
     1741 catcattcct tttgatgagg tgcttccgct gctaaaagaa gaagccctga aaagctatgg
     1801 acataaatca atggctatcg tagaaaaaaa catacaagca atcgatcgga cgaccgaact
     1861 gcttcatcaa gtcgcgatcc cagaccattg gagaacgttg gagatgcctg aaaaaatcaa
     1921 taaaaggaac actggttata tacaagaaat acttgaacca gtgaatgcac aggaaggaaa
     1981 tcaactctct gttggtacac ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg
     2041 aacggcttct atggaaaagc ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg
     2101 ctgtacgatg tgtaatgaat gtgcctttgt ttgtcctcat gctgccatcc gtccattttt
     2161 agcagatgaa gaggaaatgg aagaagcgcc agaaggattt atcgtgagag aaatgcgagg
     2221 cgcagacggc gtaaaatacc gtatccaagt ttcagtggaa gactgtacag gttgtggtct
     2281 gtgtgtagag gcttgtcccg caaaagaaaa agctttagtg atgaagcctt atgaagaaca
     2341 gaaagaacaa gctgtgaatt gggcttttgc aatgacatta agacaaaaag caaatccagt
     2401 caaaggaaag aatactgtct tgtctacaca attcaatcaa ccgcttcttg aattctcggg
     2461 cgcttgctca ggctgtggcg aaacacctta tgtaaaatta ttgacacaaa tgttcggtga
     2521 tcgaatgatg atcgcgaatg cgacaggctg ttcttctatt tggggcggtg cttctcctgc
     2581 ttctccttat acgaccaatg aatgtggtca agggcctgca tggtcaaatt ctttattgga
     2641 agataatgct gaatttggtt atggtatgct tctagcggca cagactcgtc gcgaggcttt
     2701 ggcaagttca atcaaagaag tattgccaaa agtatcagaa gaactacgag aattgatgga
     2761 agactggtta gtacacatgt ttgagagtga aggaacgcag caaagagcag caaaactgca
     2821 agctgcgatc gaaagcgaac gatcaatttt ccctgaactg gaggatatat acaagagaaa
     2881 ggatctattc gtaaaaaaca gccaatggat gattggtgga gacggatggg cttacgacat
     2941 tggttttggc gggatcgatc atgtacttgc cagcggagca gacgtgaata tccttgtttt
     3001 ggataacgaa gtctattcca atactggcgg tcaaacctca aaagcaacac cagcttcggc
     3061 aatcgctaaa ttttcggcta gtggaaagta tgcttcaaag aaagatctag gaatgatggc
     3121 gatgacatat ggaaatgttt atgtggcgca aattgcttca ggtgccaatc aaatgcaaac
     3181 gatcaaggca tttgaagaag cagaaaagta tcctggtcct tctctgatta ttgcctatac
     3241 gccttgtatc acacatggct tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga
     3301 agctgtttct tctggttatt ggtcattata tcgatataac cctacaatgg tcgaaaaaaa
     3361 caagaaccca atgactttgg actacaaaaa acctgatttc tctgctatgc ctgatttcat
     3421 gaggaaacaa gttcgctttt cctcgctaga taacgcaaat ccaaatcttg caaaagcttt
     3481 atttgaaaag actgtccacg acgctaaaac acgtttttac aactatgctg cactgacggg
     3541 agaattagaa aaaatcaaga aacgacttga accagaggaa tctatcgatg tacccgttaa
     3601 gactagaaaa gaaaaaacga cggaccttga agcagaagaa agaagagctg ctagaagagc
     3661 tgccagagca gaacgacgta aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata
     3721 acagaaaaaa tataatgaat aattctctga agtttaaaaa tatttattgc agagaaaaat
     3781 aaaacgcata cttgcaattt tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca
     3841 tatcagcctc taaaattaaa acatatgata tactagagag gaaatctcaa aagagaagag
     3901 aggcttttta tgtcatttca aatcggggaa ctttttaatt tggactattg gcagcaaatg
     3961 ttttccggtg acttgttctc tctgaatttt ttggtgaaca tacttgatat tctggttgtc
     4021 tggtatcttg tatacaaact gatccaactg ctgcgaggca caaaagcgat tcagcttctg
     4081 aaaggtgtag ctatttttat tgttatccga atcgtagcgg aaatcattgg tctgcacacc
     4141 ttgtcttggc tgatgaacca agtcattacg tacggggtga tagctgctat cgtgattttt
     4201 cagccggaaa tccggagagg actggaacat cttggacgca gttcgctttt caaacaatca
     4261 aaaagcgaac agcaggaaga tgagaagatg atcttgtctt ttgacaaagc catccaatat
     4321 atgtccaagc gaaaaatcgg tgcgttgatc actatcgaac gtcatactgg attagatgag
     4381 tatattgaga cagggattgc tttagatgca gatatcactg gggagctgct gatcaatatc
     4441 tttattccta atacaccgct gcatgacggt gcagttattg taaaagaagg aaagatcgca
     4501 gtagcaagcg cctatcttcc tctatcagaa agtatgttga tccctaaaga attcgggact
     4561 cgacaccggg cagctgtcgg tataagcgaa gtcagtgacg cgattaccat cgttgtctct
     4621 gaagaaaccg gggacgtcag catcacgtta gataatgaac tgatggctgg cttatcccag
     4681 caagaatatt tggcaatctt gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag
     4741 aaaaacgtga ttcaatcttt cctagacggt atgactaagg gaggggatcg caaatgatct
     4801 caaaagagaa acggacaagc attctttaca gttttattgc attgctgttc agtattgcgc
     4861 tgtacttcaa tgcaaatggc cagtccgttc caaatacgct ttctggaaat gaagcgtata
     4921 atcaaacagt tacgggggtg ccgatcaaga tttcttatga ttcaaagcgc tactatatcc
     4981 atggatatga aaatgcagtg actgtcaaac tttccagtgc aaaccgagtt cagttgaatg
     5041 cagaggcaaa cgaagataca cggatgttcc gcgtcacagc agatatcact aagctgggcg
     5101 aaggaacaca tgaagttccg ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga
     5161 tcgaaccatc gactattaca gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg
     5221 aaacgttgat tccttcaacg ttcacaccat ctggatatga acttgatgat acttccgtta
     5281 gtcctaaaaa agtctctatc actacaggtg acaaaacatt ggcagaaatc aaacgagtag
     5341 tagctgatgt ggacgcttcg atggtcacag atgatgggat taatagtgag gtcccaatac
     5401 aggcgctgaa tgcagcaggg gaagtattaa gtatcgtctc tgatccagtg caagtaacag
     5461 tgagagctga ttcgatcaag ccaagcaagt cggtacgact ttatgggata cagcaaggaa
     5521 caccagctgc cggggtagaa agctatgatt tcagtttttc aaaactggaa gcagaagtaa
     5581 gcggctctag cgatttgctt gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct
     5641 caggtatcaa tcatcgaaca actcgtaaaa ttgaaatacc agtagaagag gggttaagta
     5701 tcaagcccga atcagttagt gtggagatta cacccgtgtt gcaaacgtca agcagtagct
     5761 ctgcttcgac gagcagtcgg acacaagaaa atacaagtac gagtagaagt atttcatctg
     5821 ctgattcagg aacggaacca tcgacaacaa ccatttcttc cagttcggaa tctgggacaa
     5881 gcagctcgac cacaagtaca gaagaaacat cagaaacaag cgaatcaaca caaatgagca
     5941 gaacaaattc ctcagaaaat taagttaaag gagtaatttt acaactatgg gtaaatattt
     6001 tggaacagac ggcgtacgcg gtgaagccaa caaggaactt acgccagaac ttgcatttaa
     6061 gctgggacgg tgcggaggtt atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg
     6121 cgtgttagta gggcgggata caagaatctc aggtcaaatg ttagaagaag cgctgatttc
     6181 aggactttta tcggtgggta tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt
     6241 tgcttatctg acacgtttgc aaaaagcaag cgcaggggtc atgatttctg cttctcataa
     6301 tccagcacaa gataatggaa tcaaattttt cggtgcagat ggttttaaac tagtagatga
     6361 ccaagagcta gaaattgaag cattattaga tgctgaagtg gatgaattgc ctcgtccatc
     6421 agctgaagga ttgggaactg tagaagaatt tccagaaggc ttattgaaat attctcaatt
     6481 ccttgtacaa acgatcaacg gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa
     6541 cggagcaact tcaacaagtg ttaatcgttt gtttgccgat cttgaaacag atttttatac
     6601 aatgggcaca aatccagacg ggttaaatat caatgacggc gtaggctcta cccatccaga
     6661 acgtttagct gagatggtcg ttgaaaaagg agcagatgct ggactagctt tcgatggcga
     6721 tggcgatcgt attattgcgg tggatgaact tggaaacatt gtcgacggtg ataaaatcat
     6781 gtttatctgt gctaaatact tggcagaaaa aaatcgattg aaaaaagata cgatcgttac
     6841 gactgtcatg agtaacttag gattccacaa agcggtggaa ggaatcggtt tgaaagatgt
     6901 cgttacacaa gttggtgatc gttacgttgt agaagaaatg cgcaaaaacg attataactt
     6961 tggcggcgag cagtctgggc acatgatttt cttagatttt aacacaactg gagatgggat
     7021 gctttcagga attcagttac tgaatatcat gaaacagaca ggtaagaaat tatcagagct
     7081 tgcagcagag gtaacaatct atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa
     7141 cggtgcgatg gatgttccgg caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg
     7201 atcagaagga agaatcctag ttcgtccttc cggtacagaa ccattacttc gagtgatggc
     7261 agaagcgcct actgatgaga aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa
     7321 agaagaaatc ggactggatt aagagatatt ttcataaaaa ataaaagaac aaggaccagt
     7381 gtcagatgtc tgacactggt ccttgttctt ttattattct actgtcactg atttcgctaa
     7441 attacgtggt ttgtcaacgt cgaatccccg taatagcgtt gcgtaatatg caagtaattg
     7501 agtcggtacg actgtggtta gagtagttag ataagggtgg acagttggta gcaccagctg
     7561 gtcactttcc ttttctaatc cctctgagac aataactagt gttttagcgc cacggctttc
     7621 gacttctttc aggtttcctc gagtatgcgc accagtcact tcttcactga tgattccgat
     7681 cactggtgtt ccttcttcga tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa
     7741 accttctgcc tggatataac tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt
     7801 gtagtcagca ccacgtccaa tgtaaaaggc atttcttgta tcgctgagat atacttctgc
     7861 caactcttct aaataatctt tttgggcgat gaccgattcc atcgcattag caactaaact
     7921 caattcatga gtgatatcga atacaagaga agtttcaaat tcttttttat caccgatagc
     7981 tttggctaat aaagttagta cagctatttg tgcagtataa gctttcgttg atgctactgc
     8041 gatttcgggt ccagcatgca atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc
     8101 agcaacattt gtaatcgtaa gtgaagggta gccgagacgg ttgattttta ccagcacttg
     8161 gcggctgtct gctgtttcgc cactttgact caagaaaata aagaatggtt tttttgaaag
     8221 aagaggtgtg ttatagccaa attcactggc taaatgaacc tcaacaggaa tctgtgtcaa
     8281 tttttccaaa gtctgtttac ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat
     8341 atagattcga tcgctatcag acaatgtatc gaccagcttt tgatcaagag agacacgatt
     8401 gtcttctgtt acgtattttt gagcgatacg acgcatgata ataggctgtt cgtcgatttc
     8461 tttcagcata taaaaaggat aggtaccttt ctcgatatct gaagcatcta cttgtgcttc
     8521 gaatgaagaa cgttctacga tgtcacctgc aaaattttga ataataactt tttctgatgt
     8581 gacggtaacc atctcttcat cttcaatctc aacgaattcc ttcgtatgag caagcatagc
     8641 cattgcatcg ctggcaatca cattgaatcc atcgccgagt ccgattaata gtggactttt
     8701 atttttagcg acgtaaataa catcaggtgc tgtccgatca atcaaagcga aagcgtaaga
     8761 acctttgatg acccgcaaag cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa
     8821 agtttcaatt agatgagcga taatctcagt atcggtctca ccaataaagt ggtcatttgc
     8881 taaataagca attttaagtt cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa
     8941 gcgtccactt tgggaagtat gtggatgggc gttttcttca gaaggttgcc cgtgagttgc
     9001 ccatcgtgta tgaccaatac ctatatttcc tgtagtatca atatttattt tgtttttcaa
     9061 attttgaatt ctgccttgtg ctttgaccag ataatcagtt gtgccatctg agacaaagat
     9121 accagctgaa tcataaccac ggtattctaa tttttctaga ccgtcaatca atcctggtgt
     9181 tacatttttt aatccaatca ttccaacaat tccgcacata aaaaacatcc tactttctat
     9241 aatgaattcg tatgaatgct tccagctgca ggaaaatcct tttgttttat ctttgcagac
     9301 attttttgtg gattttctgt agagtgcagt ccctagggcc atccgccgaa tctttcgata
     9361 aaccctctcc tcgtcatctg tttacacaga cctggcgcta tcctaaaagg tataactatc
     9421 ctcctttttg ttttttatct ggaaacattt aaaatcataa ccttttgcga atgttcttgt
     9481 caaca
//
LOCUS       DACCXW010000110         9315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCXW010000110 DACCXW010000000
VERSION     DACCXW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.113"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(46..714)
                     /locus_tag="IVY71_002260"
     CDS             complement(46..714)
                     /locus_tag="IVY71_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7986299.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            848..1135
                     /locus_tag="IVY71_002261"
     CDS             848..1135
                     /locus_tag="IVY71_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986300.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1551..3089
                     /locus_tag="IVY71_002262"
     CDS             1551..3089
                     /locus_tag="IVY71_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7986301.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3086..4051
                     /locus_tag="IVY71_002263"
     CDS             3086..4051
                     /locus_tag="IVY71_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986302.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4038..5360
                     /locus_tag="IVY71_002264"
     CDS             4038..5360
                     /locus_tag="IVY71_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7986303.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5423..5527
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5601..6344
                     /locus_tag="IVY71_002265"
     CDS             5601..6344
                     /locus_tag="IVY71_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7986304.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6359..7351
                     /locus_tag="IVY71_002266"
     CDS             6359..7351
                     /locus_tag="IVY71_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7986305.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7363..8100
                     /locus_tag="IVY71_002267"
     CDS             7363..8100
                     /locus_tag="IVY71_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986306.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8090..8812
                     /locus_tag="IVY71_002268"
     CDS             8090..8812
                     /locus_tag="IVY71_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7986307.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8896..>9315)
                     /locus_tag="IVY71_002269"
     CDS             complement(8896..>9315)
                     /locus_tag="IVY71_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7986308.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3053 a         1604 c         1967 g         2691 t
ORIGIN      
        1 ataatgatat ttctgtaaat taaataaagt agtgaaaaat aagaatcagt gctctaactg
       61 ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt tttgcttcca attggggcaa
      121 aacagatcca atttccagat acttcacttt attcaatgta taagatagaa tagaacagct
      181 aagtaatgta tacagaaaat gtgtgagatc caaataaagc aaggagacaa ctaaaatacc
      241 aaaagtaatc aagaaaagac ttttgccaat cttaatatga aaatattttt caatggcttg
      301 ataaatgata tctgttccgc ctgtgcttcc gccgaatcga aagacgagac cgcaaccaat
      361 gccagaaaga atacctgtca tcaaactatt gatcagtaga ctatctacag caaaatgtat
      421 atgaaggctc tcccataaat gtaaaaagaa aactagcgca aatgttccga cgatcgtccg
      481 gagaaaagcg tgttttccaa aaagaaacaa tgagataagc aataaaggta aattaaaaag
      541 cagtgaagtg acagaaggag cgaatccaaa aagattatgc aaaagcaaag caataccagt
      601 aaccccacca tctgccagct tagatggaat attgattcct gtcacagcta atccgtagat
      661 tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa tttaattttt tcatttctag
      721 cctcttttct tttgaaaatt ttcagcaact tctttattat actgacttat actcttactt
      781 caagaggaaa atgaaaaaaa attatttttg gctgtttctt tttatttatc aaataaggag
      841 tggaaaaatg cgagcagcag acttattgac aattcttaca aaatcaccca aaaaaacaga
      901 gtatcaagga gtttttttgg ctcaagaaca aaaactggtg cctgtgacaa tggtccagat
      961 aaataaagca aatcaattta tctttttatt tgaacacggc aaagcaccac ttgctatgaa
     1021 agacatcttt ctttttttga tgaagaaccg tgagaaagct gtttattatc agtcaaaaga
     1081 agaactcatt cctctttatg gagtgaaaga gatagaaaat aaagtggtga tataagtaaa
     1141 aataagaaaa caagacgttt atgatgtcaa aacaaccatt ttttacattt ttggcaaaaa
     1201 agtgtcatga tacagtataa tattcgttga acaggccaat aaaggtgttc tcccccacga
     1261 gatttctact ttattggctg tttcttttta ttaaaataaa agttgtgtgt gtaaaaaaga
     1321 tatagctgat tccaagtgac aatttcgtat agatttttat ggtattcgta gtgaaaaaac
     1381 gagaaccaaa aagttgatcc gactttgttg cttggattct ttttttgtca gcatatttag
     1441 aggtattttt ttcttttttt tgttcgtgct gtaaggttaa ggcaagacaa gttagttggg
     1501 aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa ggaaggtcgc atgaataatt
     1561 ttatacaaac attaagtgaa agaaaaggcg agttgctgga agctgcattt caacatctgt
     1621 ccatttctct catttctttg ctgattgcag cactgattgc tattccttta gcgatttggg
     1681 cagccaatca taaaaaaatg gcagaagtct tattgcagat cacgggtgtg ctccaaacga
     1741 ttccttcact tgccttatta ggtttactta tcccatttgt agggatcgga actgttccag
     1801 cattgattgc acttgttatt tacgctttgc ttccgatttt ccaaaacacg tatattggtc
     1861 tagctgaaat tgacccgtct attgaagaag cagcggtagc atttggtatg tccagaatga
     1921 gacgcttgct gaaagtagag cttcccatcg cattacctgt cattatctca ggtatccgaa
     1981 ctgccttagt tttaatcatc ggtacggcta ctttggcagc attgatcgga gcaggtggat
     2041 tagggacatt tattttgtta ggtattgacc gaaatacacc tgttttgaca ttgattggtg
     2101 cgatcagttc tgccttgctt gcaattatat tcagcggact gattcgctgg ttgcagcata
     2161 agaaacctcg atattccatc attactttag cagcagtggt gtgtttattt ggcggcgttt
     2221 ctttatatca gtctgccttg tttaaaaatg aaaccatcac gattgctgga aaattaggag
     2281 cggaacctga aatcctgatc caaatgtata aagaattgat tgaagacaac acaaaaacag
     2341 aagttgaatt aaaaccgaat tttggcaaga cgagtttttt gttcaatgca ctggatcaag
     2401 gtcaaatcga tatctatcct gagttcacag gtactgtcct agaaagcttg gtgaaggtac
     2461 ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac atatgacgaa gcagaagaat
     2521 tattgaataa gcaattcgat atgacattat taaaaccaat ggcttaccaa aatacgtatg
     2581 ccttggcagt caagagatct tttgcgaacg aacatcaact taaaaagatt tccgatttgg
     2641 cagctatctc tgatcagatc aaagcgggat tcacactgga atttattgat cgttcagatg
     2701 gatataaagg aatccaagaa gcatatggct tgaatttttc aagtgtacaa agcatggagc
     2761 caagtttgcg ttaccaagca atcaataatg gagatgtcaa tattgtggat gcctattcca
     2821 cagatagcca gctcaaggag tacgatctag tgactctaga agatgatcga gggttattcc
     2881 cagcttatca aggcgcaccg ctgatgaaaa aagattttgc taaagaacat cctgaaatcg
     2941 ttaaagcact gaataaatta agcggaaaaa taacagagga tcaaatgatt gatatgaatt
     3001 accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc acgaatcttc ctgaaaaatg
     3061 aaggattagt aaaggaggaa gatcagtgac taaattaatc gagttcaaac atgtgcaaaa
     3121 aaagtatgat ggaaagtatg tcatcgatga cctaaatctg gcaatcaaaa aaggagaaat
     3181 ctttgttttg gttggtcctt ccggtagtgg aaaaacaact actttgaaaa tgatcaatgg
     3241 attatctaaa ccatcagcag gagacatcta cttcaaagga aaatcgttaa atgaatataa
     3301 tctgcagaaa atgcggtgga atatgggata tgtgctgcag caaatcgctt tgttcccgac
     3361 catgacagtc aagcaaaata ttgaagtcat ccctgaaatg ttaggttggg aaaagcagaa
     3421 aagagcagac cgtgtagatg aacttcttca aaaagttggc ttatctcctg atatttatcg
     3481 agatcgtatg cctagagagt tgtctggagg agaacagcaa cggatcggga tcatccgagc
     3541 tattgctgca agcccagatg tgattttgat ggacgagcca tttagcgcat tagatccgat
     3601 ttcaagaaac tcattacaag aattggtttt gtccttacac gaagaattgg gcacgaccat
     3661 cgttttcgtt acgcacaata tggaagaagc aatcaagcta ggtgatcgta ttgcatttat
     3721 gaaggatgga gaaattatcc aatgtgacac accagagcag ttattgatga atcctaaaaa
     3781 cgactacgtt cggcatttct ttgatgaacc gaaacagacg aaagagtggc gagtagaaga
     3841 tttagtcgtc aatggatatt tcttaaatga aataccagaa aatgctcgtc aacaagtttg
     3901 ctttgatacg cctatgaaag aagtatattc gcttctttcg gtgtatccaa gcattgtgat
     3961 agtagaaaag caaagaagta ttgggtcgct gacaagtaaa gaaatctttg cttttttaag
     4021 tcgagaggag gaaggaaatg aaaacatatg atgcaatcat catcggttca ggtgtgagcg
     4081 gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac agtactagtg gtcgaagaag
     4141 atctatgggg cgggacttgt cctaaccgtg gatgtgatcc taaaaaagtc ttgttaagtg
     4201 cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa aggttttaac gagatcccaa
     4261 ctgccaactg ggaagaactt caaaaattca aacgaacatt tacggatcca gtcccagaaa
     4321 gccgaaaaaa acaattagca gaagctgaaa tcgatcatct ttcaggaacc gctcgttttt
     4381 tggatgattc atctattgaa gtcaacgaag aagtcttcca tgcggattac ttagttttgg
     4441 caaccgggca aagacctacg atcttaccag tggagggaaa ggaatacttg aaaactagtg
     4501 cagacttttt atcattgcct gtactgccta aagaaatcat ttttatcggt ggtggttata
     4561 ttgcttttga gctagctacg attgccaatg ctgcagggag caaagtaacc attgtccatc
     4621 ataatcaacg gccattaaaa gaatttgagg cgtctttggt agaggaagcc gtccatcaaa
     4681 tggaagcatc tggcattcag tttgcttttg gtgttgaaac ccaaaagatc atttcagaag
     4741 gtactcgcta tcgtttagtc ggaaaagaga cggaattagt agcagatatg attttttgtg
     4801 cgactgggag acagccgaat acagaatctt tagcacttga gcaagcgaat atcgtctttg
     4861 ataaacacgg aattgcagtc aatgattatc tgcaaacgag caatccaaaa atttttgcgt
     4921 gtggagatat tgtttcaagg aaaaccccaa aattgacacc agtcgccact tttgaaggaa
     4981 attatgtagc taagcggata acggatgcaa caagcgaacc tatcaaatat ccgattatac
     5041 ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg agttacaaaa agtcacgcct
     5101 cttcgtcaga tcaagtggta gagatggatc ttaccagttg gttcacctat catcgtgtca
     5161 atgaaccagt tgctaaggct gaattgacct ttgaccagca aaattatttg attggtgctg
     5221 cagtgattag cgagcaagca gacgaattga tcgatgattt gacactcgtc atcaatcaaa
     5281 aattgactaa aaaagagttg gatagctata tcatggggta tccgacattg gcaagtgatc
     5341 tctcctattt attgaaataa tcagcatgat aactaaagcg cttgttgctt tttccttctt
     5401 gtgctacact ttaaaaaatg aatacctgtt tctggggatg tcgtagacga catgagagta
     5461 agttgtgaac ttagacccat tgaacctgtc agttaatact ggtgaaggga gaaacatagc
     5521 agacgcttga agcgggttac tatgtcgtgc ccatttcaag cgttttttgt tattcatatt
     5581 agaagaaagg aggtaacaaa atgaagaaaa atctttatcc agcagtcagt atggcattca
     5641 ttttaattat ttggcagatt gttgtcatag tttggaaaat acctgccttt attttacccg
     5701 gacctgctgc tgtattttct gcattagcgg cagatgcaca agtattactt tcccattctg
     5761 tcacgacatt aaatgaagca gtgcttggtt tggtaatagc tagcgttctt tcttttatta
     5821 ctgccttggc aatggattat tggaagtttt tagaaatgtc tttttatccg ttactagtga
     5881 tttcacaaat gttgccaatc atggtgttag ggccattact taccctatgg tttggtttcg
     5941 gtatgcttcc aaaagtgatc cttgttgtct tgataagcta ttttccgatc gtagtttctt
     6001 tttcggatac attgaggaaa gtatcaaaag agcaaatcat ctttttgaaa acgatggggg
     6061 cagatacagg gaagatttat cgaatatata aaataccagc gggtttatcc ggcttctttt
     6121 ccggtttaaa agtggctgct acttattgtg tcagcggagc agtcgttgga gaatggctaa
     6181 gcgctcaagc aggcttaggg tattacatga tacgcgtaaa aaacagctac cagatcgata
     6241 aagtttttgc tgcgatcatt tgtgtcattc tattgagttt attgctgaat ggcgcttgtt
     6301 cattattgaa aaaagggtat tatcgtatgc tttatatttt ttaagagaag gggatcaaat
     6361 gaaaaaatta tggcttttat taccgctact gttgctttta tccgcttgtg gcactgcaaa
     6421 agaaacaagt agcaaacaag aaataaagga tttgaaaaaa gtgacacttg ttttggatta
     6481 tgtaccgaat actaatcata ctggcatcta cctagcaaaa gaaaaaggct attataaaga
     6541 ggctggttta aatgtccaaa tcatcgaacc aggagataat tcgacaagta ttggtctagt
     6601 aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa gatgtcactt atgctcacgc
     6661 agatggacag aatataccag tcaaagcaat tgctacagtg atcaaacata atacttctgg
     6721 attcgccacc ctatcagaca gtaacatcca ttcaccaaaa gattttgaag gaaaaacata
     6781 tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa gctgtgatgg aaaaagacgg
     6841 cggggacttt tcaaaactga caatggtagg aagcaatgga gaagggccag aaagcttagg
     6901 aaaatcctca gatatccaat ggtactttga aggttgggat atgatcaaag caaaagaagc
     6961 gggtattgaa ctaaactata tcccattgaa agaattagat gaacggttag attactatac
     7021 tccggtaatt attacgaatg atcaactgat caaaagtgat ccagaattgg tacagtcttt
     7081 tatggatgcc acgaaaaaag gctatcagga agctattaag gatccaaatg acagtgctaa
     7141 actgttgcaa aaatatgcaa aagaaaatga tcggacgttt ttagaagaat cacaagcatt
     7201 cttgtcaaaa aattatacgg atgatcctga gaattgggga ttgatggaag aaaaagtttg
     7261 gcgcaattat acagctttta tgcaggaaaa cggattgatc aagcaagatg taccttcgca
     7321 agaactattc actaatcagt ttataaaata ggagtgatag atatgtcaat ccaaatcagc
     7381 cacttgaatg tagtcattga aaacctgaaa atattagaag acatccattt cactatccct
     7441 aaagatacct ttacatcgat tgtcgcgcca agcggagccg gtaagtcaac attgctgaaa
     7501 actttaacag gagtccagcc tatgacttct ggaacgatca aagtagacga tcaaaaagta
     7561 actggtctcc atacagcatt tagttatatg ccgcaagaag atatgttact tccttggctg
     7621 agcgtctatc aaaacgttac gttatatcaa aaaatcaatc atctaacaat caatgaagaa
     7681 caagtaaggg attatctgga tatctttggg ttaaacggct atgaacattt tttgccggaa
     7741 caattatctg gcgggatgaa gcagcgaacg gccttgttac gaacaatcat gaatccttct
     7801 tcttacttgc ttcttgatga accatttgga gcgttagatg ctatgaccag agggcagatg
     7861 caagactgga tgttgaagct gcctaaaaaa gcaaagcgga ctactttact tgtgacgcat
     7921 gatatcgaag aagcaattta tttatcagac aggattcttg tgctttccgc ccgtccggca
     7981 catgtgattg ctgagattca agtgcctgaa aaacgacggt cacgtgaatg gctactgcag
     8041 caatcagagt tgaagcaaac gatttatcag ttattggcag gtgaattaga tgttaagtga
     8101 tgctcattgt catttagatg ggagtcagag tttagcactc cttcagcagg aacacacgat
     8161 tttgacgatc atcaattgtg attcgccaga agaatggaag gaaaacagac agcttgcagc
     8221 aagtaagact caagccttaa gttacggtat ccatccttgg aaagcagata gctatacgtt
     8281 tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc ggtgaaatag ggttagataa
     8341 tatttggacg aatgtaccga tgactacaca aaaaaaagta tttgagcgac agcttgcttt
     8401 tgcagccata aatgagaaac ctgttgtact gcacacaaaa ggatgcgaaa aagagatctt
     8461 aaattatatc caagcttacc ctaatcacta tttgatccat tggtattctt ctttggacta
     8521 tcaacaagac tatattgatt taggctgtta tttcagcatt ggtcttgatt tgaaaaagaa
     8581 tccagcagtc tggcaactag ctaaagcagt cccaatcgac cacttactta ttgaaacgga
     8641 tggactacat gcagcagagt gggctttagg ggggattttt tcagaaaaag attatgtctc
     8701 actgcttgaa gaacagttaa agacaatcgc tgagatcaaa gagctatcag ttgaagaagt
     8761 taaaaaaaat acttatcaaa acttggaacg tttcattaga ttaggtaaat aaaaaagaga
     8821 tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt gtataaaagt aaactgtttg
     8881 attaataaat gaatcttagt accaaccgtt tgcttcccag aatgctttcg ccgcttccca
     8941 agaaccataa cgtgaagtaa catattgttc tgctactctt tcttggtttg cagcagagta
     9001 gtcaccattt aagtaagaag aatctaattg gtaacgtccg atgtaacgtc cgttcgttgc
     9061 tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct tttgcagagc ttgtgcttgc
     9121 tggagtagca gcagtttctg ttacagcagg agcaggttgt gtttcagcaa caggcgctgc
     9181 ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt tcagcttgga ctggtgcttc
     9241 ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag ccttctgttg ggattgttaa
     9301 ttgttgtcct gagta
//
LOCUS       DACCXW010000111         9134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCXW010000111 DACCXW010000000
VERSION     DACCXW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.114"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            22..252
                     /locus_tag="IVY71_002270"
     CDS             22..252
                     /locus_tag="IVY71_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7986309.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            349..1650
                     /gene="murA"
                     /locus_tag="IVY71_002271"
     CDS             349..1650
                     /gene="murA"
                     /locus_tag="IVY71_002271"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7986310.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1663..1839
                     /locus_tag="IVY71_002272"
     CDS             1663..1839
                     /locus_tag="IVY71_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7986311.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1974..2270
                     /gene="yidD"
                     /locus_tag="IVY71_002273"
     CDS             1974..2270
                     /gene="yidD"
                     /locus_tag="IVY71_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7986312.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2334..2801
                     /locus_tag="IVY71_002274"
     CDS             2334..2801
                     /locus_tag="IVY71_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7986313.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2886..3770
                     /locus_tag="IVY71_002275"
     CDS             2886..3770
                     /locus_tag="IVY71_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7986314.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            3887..4120
                     /locus_tag="IVY71_002276"
     CDS             3887..4120
                     /locus_tag="IVY71_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7986315.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4208..4288
                     /locus_tag="IVY71_002277"
     tRNA            4208..4288
                     /locus_tag="IVY71_002277"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4242..4244,aa:Tyr,seq:gta)
     gene            4295..4366
                     /locus_tag="IVY71_002278"
     tRNA            4295..4366
                     /locus_tag="IVY71_002278"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4327..4329,aa:Gln,seq:ttg)
     gene            4882..5589
                     /locus_tag="IVY71_002279"
     CDS             4882..5589
                     /locus_tag="IVY71_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7986316.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5648..6292)
                     /locus_tag="IVY71_002280"
     CDS             complement(5648..6292)
                     /locus_tag="IVY71_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7986317.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            6419..6706
                     /locus_tag="IVY71_002281"
     CDS             6419..6706
                     /locus_tag="IVY71_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986318.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6814..7134
                     /locus_tag="IVY71_002282"
     CDS             6814..7134
                     /locus_tag="IVY71_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7986319.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            7452..8528
                     /locus_tag="IVY71_002283"
     CDS             7452..8528
                     /locus_tag="IVY71_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7986320.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            8525..9134
                     /locus_tag="IVY71_002284"
                     /pseudo
     CDS             8525..9134
                     /locus_tag="IVY71_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
BASE COUNT         3024 a         1514 c         1976 g         2620 t
ORIGIN      
        1 atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg
       61 agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact
      121 tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt
      181 ttagggtaca ctgcaagtaa ttttttctta gaggttatcg ctttatgccg aaatcttttc
      241 gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa
      301 agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc
      361 atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct
      421 gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac
      481 gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat
      541 gtagattttg atgaacaaaa gaatcaagta acaatcgacg cttcaagaca attagaaatc
      601 gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta
      661 cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga
      721 ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac
      781 ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct
      841 agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc
      901 attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg
      961 ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta
     1021 catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct
     1081 gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca
     1141 ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt
     1201 gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc
     1261 cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgtg
     1321 gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac
     1381 gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt
     1441 gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa
     1501 gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc
     1561 catatcaaac tacagcaatt aggtgcagac gtcgaacgtg tagacatgga tcaaacttct
     1621 gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat
     1681 atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg
     1741 gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag
     1801 ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt
     1861 ctagtcgata tgaagtgagc catgaagcag aacatgataa aagaaaacag aagggaacgt
     1921 tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga
     1981 agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta
     2041 gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg
     2101 cgctaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag
     2161 gcggtatcga ttatgtacca ttgacattcc gattaacgcg aaataagaaa gataaaaaag
     2221 aaccagatta taaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag
     2281 gaaatttatt gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg
     2341 aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag
     2401 aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa
     2461 caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag
     2521 taaccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga
     2581 agctttcaca gctagcttca aaccaaactc ggatcattat acgtactggc actactagta
     2641 gcgctgcgtt acacctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact
     2701 attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag
     2761 tgattttagc aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa
     2821 aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata
     2881 tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg
     2941 atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga
     3001 aaacgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg
     3061 gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga
     3121 ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt caactcccag
     3181 taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc
     3241 gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca
     3301 cgaaaggaca aataaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct
     3361 tagatggcga taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt
     3421 gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag
     3481 aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg
     3541 gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt
     3601 ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat
     3661 tcgatctgca tgatttcgaa ccgcagattt tattatgtga gtacagaaac gatgctaatc
     3721 ttataggtgc agcagcaaac tatctcgctg tacgtcaagg agagaattaa gagctttaga
     3781 gaaggtgcag cttgcataaa agaagagaat gaaatgaata ggagattttt acctaaggaa
     3841 tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa
     3901 taaagatatt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga
     3961 agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa
     4021 agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc
     4081 gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt
     4141 caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa
     4201 acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg
     4261 ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa
     4321 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt
     4381 aagctctcgt tagttcgaga gctctttttt tttgctttgc taccttgttc cttgaagatt
     4441 ctgctgaatt aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg
     4501 aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac
     4561 aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc
     4621 ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat
     4681 aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa
     4741 aaaattgttt ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg
     4801 gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa
     4861 acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac
     4921 caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa
     4981 agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac
     5041 ttggcagatg aaggaatctt agaaagaaag atcggttcgg gtacgtatgt tgcgcgagaa
     5101 aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat
     5161 cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg
     5221 gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca
     5281 gatgatgtcc ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac
     5341 agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag
     5401 atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat
     5461 ttagaggtaa aacgaagaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac
     5521 ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg
     5581 gaaaaataaa aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca
     5641 cctgttttta tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa
     5701 attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtta gcactttttt
     5761 catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat
     5821 gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga
     5881 tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat
     5941 gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga
     6001 ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg
     6061 ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga
     6121 tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaat
     6181 tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga
     6241 tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac
     6301 ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
     6361 acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat
     6421 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
     6481 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
     6541 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
     6601 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
     6661 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
     6721 gaaaacgttg acaaatttct ttctttttat ataataaagt aacagacgcg aagaaaacgt
     6781 ttgcgtagtt tgttttaaaa aggagagaaa aatatggcta aaaaaacaat catgcttgta
     6841 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
     6901 aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
     6961 aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt
     7021 gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt
     7081 atgatgaatg gcgaaaaagt gttagatcaa gcaatcgcgc tcataggtga ataaatagaa
     7141 cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt
     7201 ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag
     7261 caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt
     7321 tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt gttggacagc
     7381 ttgcagatta cggttatttt tgtacacatt gtctatattt agggtgatgc gacactcaac
     7441 aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga
     7501 caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc cattcagaaa
     7561 aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta
     7621 ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg
     7681 tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc
     7741 tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc
     7801 agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc
     7861 cttataacgg ggatgctggg ctagctcacg ctgtgcaaac agcaatcaga tcaaacggcc
     7921 gattcatcta cttaagtgcc acgcctgatg agaagttact aaaggaaatc aagcagacat
     7981 ttgagctcca caaacttgcc ttgcgctttc atcggcgctt gcttcctgaa cccgtgcttg
     8041 ttttcatgaa taattggcga caaaaatgtg gaaacaaaaa gaaaatcaac tctttggtgc
     8101 ggatcattca gcaactaatc aaagaaaacc gtatcctttt attttgtcca agtattacga
     8161 tgatgcatcg tttaaaaatc acactgcaag aagcttttcc agaatatgag ataacagatg
     8221 tttcttcaca agacgatcaa cgggcaaaga aagttcagaa aatgcgagaa gcaatatacg
     8281 agatactgtt aaccacaatg atcctagaaa gaggagtcac atttgaaaga atctctgtca
     8341 tcgttttagg agcagatcat ccagttttta ctaaatcctc tttggtgcaa atcgcaggaa
     8401 gagctgatcg taaaggtcct tatacgaaca gtcgagtcta ttttttctat gaagaaaaaa
     8461 cttcagctat tcgaaaagcc tgtcaggaaa tcaaagaaat gaacgaagaa gggaagcaga
     8521 tgctgtgaga tgtagttgct gcggtaaaga catcattcaa aatttaacat tcagcgagat
     8581 catcggagtg tcgaaatcta gacttcctca gacatgttca gtttgccgcg cacaattcca
     8641 tttattagaa gcaaaaggtt gtgcaggttg tcaaaaacca atccatttga aatacgagga
     8701 gacgaaaaag aaagcaaata ctagtttcgt ttactgcaac gactgtcaag aatggaagaa
     8761 gaaatatcct gcatatgatt ttcaacaccg cgcctttttt acgtacgatg aaaagatgca
     8821 ggcttggatc aatcggtaca agtttatggg agatatccga ttagcaggga cttttgcata
     8881 taaatggact cagataaaaa aaagattacc cggcatatat ttattgtcca atacctttat
     8941 ctgaaaaaag gtggctagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa
     9001 aaattccttt tcagatgctg ctcaaaagag agcaacatct gcttgctcaa gcaaaaaaca
     9061 cgcgaaagga aagattggag atgcctcagc cttttgagct tgccgtacct ctttctaaga
     9121 tacaacataa aaaa
//
LOCUS       DACCXW010000112         8985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCXW010000112 DACCXW010000000
VERSION     DACCXW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.115"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..535
                     /locus_tag="IVY71_002285"
     CDS             <1..535
                     /locus_tag="IVY71_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7986321.1"
                     /translation="DGSKKWETCLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKII
                     YTIGSGINYGEIYSTAACWFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGD
                     TRFLDQRVEDFTKKFSNKLMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAF
                     ERGHSLDVRRYMWQMKY"
     gene            517..978
                     /locus_tag="IVY71_002286"
     CDS             517..978
                     /locus_tag="IVY71_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7986322.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1028..1492
                     /locus_tag="IVY71_002287"
     CDS             1028..1492
                     /locus_tag="IVY71_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7986323.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1506..2255
                     /locus_tag="IVY71_002288"
     CDS             1506..2255
                     /locus_tag="IVY71_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7986324.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2255..3085
                     /locus_tag="IVY71_002289"
     CDS             2255..3085
                     /locus_tag="IVY71_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7986325.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3085..3732
                     /locus_tag="IVY71_002290"
     CDS             3085..3732
                     /locus_tag="IVY71_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7986326.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(3830..4606)
                     /locus_tag="IVY71_002291"
     CDS             complement(3830..4606)
                     /locus_tag="IVY71_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7986327.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            4750..5709
                     /locus_tag="IVY71_002292"
     CDS             4750..5709
                     /locus_tag="IVY71_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7986328.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            5826..6134
                     /locus_tag="IVY71_002293"
     CDS             5826..6134
                     /locus_tag="IVY71_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7986329.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6161..6460
                     /locus_tag="IVY71_002294"
     CDS             6161..6460
                     /locus_tag="IVY71_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP7986330.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            6634..7185
                     /locus_tag="IVY71_002295"
     CDS             6634..7185
                     /locus_tag="IVY71_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP7986331.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7331..7624
                     /locus_tag="IVY71_002296"
     CDS             7331..7624
                     /locus_tag="IVY71_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986332.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            7617..7913
                     /locus_tag="IVY71_002297"
     CDS             7617..7913
                     /locus_tag="IVY71_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986333.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            7984..>8985
                     /locus_tag="IVY71_002298"
     CDS             7984..>8985
                     /locus_tag="IVY71_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7986334.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         3170 a         1300 c         1690 g         2825 t
ORIGIN      
        1 agatggctct aaaaaatggg aaacttgctt gaatgaattt gataagttaa atgatcttgt
       61 tattgctgaa aagagcaaat ttgaaataac tgctaaagaa tggggtaaaa accaaaaacg
      121 agataaaatt atttacacaa ttggtagcgg tatcaattac ggagaaattt actccacagc
      181 agcttgctgg ttcatggaaa tgcaatggat taattctaat gctattcatt ctggggaatt
      241 tttccatggt ccttttgaaa tcactgatta tgatgtacca ttcatcttag tgaaaagtat
      301 tggagataca agatttttag atcaacgggt agaagacttc actaaaaaat tcagtaataa
      361 attaatggtt ttagaccaag ctgattttga cttagaatct atagatagtg tagcgaaaga
      421 atatattgca gctgtactta gtggcgttgt tattcgccaa atggtagaag cgattgcttt
      481 tgaaagaggc cattccttag atgttagacg ctatatgtgg caaatgaaat attaattaat
      541 aggaggttgc aaaagatggc aaatcaatta tttcacattg tgattacaac acattctacc
      601 ctttgtgagg gttatttaaa ggcaacaaaa ttaattttaa tgaatgacgc cacaggtatt
      661 agtgctgttc catttaaaga aaatactagt ccagatgatt ttgaggagag tatgaaagaa
      721 ataattagtc agcatcattt gcaacctcta attattttaa cggatttaat tggaggaaca
      781 cctaataatg tttctattaa acagttgaac aacaatagaa ttcaaattat tgctgggatt
      841 aatttacctc ttttactgga gttattaatg gcccagtcag ctggtaaatc tatagaagaa
      901 atagatttta aggaattact gaataactct caaaaaagta tggttcatat taattcgctt
      961 ttaaaagatg tcagttaagg tggcaatttc tattctataa attttaaaaa ttgaaggagg
     1021 aaatagaatg attaaattag tgcgtgtaga ccacagatta cttcatggac aagtaatatt
     1081 ttcttggact aaacagtatg atattaacca tattattgtg gctgataaca aagttccagg
     1141 agatccaatg tctgtgatgg ctttgacaat tgctaaacct gctgattgca aattagacat
     1201 tgttaaaatt agtgatgtta aagggattat ggaaacatcc ccaaatgata attacatgat
     1261 tttaattaaa ggacctaagg aagcttttga attagttcaa aatttaccag aaattaaaga
     1321 aattaatttt ggcggtgtgt ctaagaaaag cgagtccaaa caatatggaa aggcagtctt
     1381 tttaactcct gaagaattag aagccacaaa aaaaattctt gatcaagaag tgaaaatatt
     1441 tgtgcaacaa gtcccatctt caagtattga aaatgtagat tttagtaagt aaggggggaa
     1501 aataaatgtt attaaaagcc attttattag gtttagttgg tgtggtagct gttattgact
     1561 ccaggttaat tggtcgacaa aatattggta gaccactaat tttgagtaca cttgccggac
     1621 ttgtgctagg tgacttgcat acagggattg ttcttggggc atctttagaa ttaatttcta
     1681 tgggattcgt ctcaattggg gcggctggac ctcctaatat gcaacttggt agtattattg
     1741 ccactgcttt tgcaatttta actaatagta gtacagaaac agccttaaca atagctatcc
     1801 cagtggctgt agctggtgaa tttatgagta ttatcatgcg catgataatt gctcaatttg
     1861 gacatgctgc agataaagcc attgaaaata ataaatttag aaaagctcaa attattcata
     1921 tttattggtc atttattttt aatgcttttg tttactttat tccaatcttt ttaacagtat
     1981 attttggtgc tgacgttgtg gcagatttag tagataaaat tccagaaatg attacagacg
     2041 ccttgactgt ctctggaaat atgctttccg cattagggtt tgcaatgcta ttaagcacaa
     2101 tgttgagtaa aaaattatat ccttacttta tttttggatt ctttattgtt gcctattcag
     2161 gtctttcact aattggcgtg acaatttttg cagctctgat tgcctttgta atggatcaag
     2221 ttaaatatgg aaaaagagaa gaggcgcatg gttaatggaa aatagtacag aaaaaaaacg
     2281 tttaatcaca aaaaaagatc ttaataagat gtgtcttcgt tcatggacaa tcatgtgttc
     2341 gtggaattat gaaagacaaa tgcatatggg atttatgtat gggatggcgc caattttaaa
     2401 taaagtttat aaaaatgatg aagagaaaaa aagagaagct tatactcgtc atatggcatt
     2461 ttttaactgt acgcctcaaa tgacttcatt tattatggga ctttgtgctt ctatggaaga
     2521 acaaaatgct caagcagaag aaggaaactt taatactgaa tctatctcca tgattaaaac
     2581 cagtttaatg ggtccttttg ctggaattgg tgattcattt ttccaaggta ctttaagaat
     2641 tattacattt gggattggat tgtcttttgc taagcaaggt agtattttag gaccgatttt
     2701 agctgttggc ctttttgcta taccttctat tttagctgtt ttttacggcg gtaaattagg
     2761 atatgagtct ggcaataaat accttactaa attgtatcaa gaaggtctta tggatcgagt
     2821 aatgcaattt gcttctatca ttggtttggc tgtagtaggt ggaatggtgg ctagcatggt
     2881 gccaattgtc acccctttaa aattcactac agggggaaca gaacttgttt tacaagactt
     2941 acttgattcc attattcctg gattattacc acttgtatgt actttgggaa tatataaatt
     3001 gattaagaaa aatgttagta caaatatgct cttattaggt tgtattttgg cagggtttgt
     3061 tttaagtata ataggtatct tataatgatt aaggctctaa tttttgatat ggacggagtt
     3121 ttaattgata gcgagattat gtatcaaaag tgggtattta attttttaaa acaagaaaat
     3181 ttaagctatc ctttgtattc ttatcaaaaa aagattggaa ctacagcaag tatttttgat
     3241 gatatagaaa agtacaatga gggatgtaac agtttagaat tgaagggaaa atttactaaa
     3301 tattggaaat cgcaaaagat tgattacttg gatatttttc ctaatatact aattgaacag
     3361 cttgaatggt taaaaaagaa aaaaataaaa atggcaattg cttcttccag tccaatggat
     3421 actataaatg aagttgttac cacttgtaaa atagagtcat attttgattg tttaattagc
     3481 ggaagagatt tgccagaaag taaacctaat cctactatat tccttaaagc ttcagaacag
     3541 ctcagaattc cagttgaaga atgtctggtg atagaagatt cttataatgg tataaaagct
     3601 ggtaaaagag caaatatgaa ggttcttgca attaaagata aacgtttttc tcaagatgta
     3661 agtctagcag atgatgttat ttacgatatt aaatatctgc gacaagaagt acaggtgaaa
     3721 tttggaatat aaataagttt tttgatgtat ttagagaata ttagtaaaaa taaaaatcat
     3781 gcccaaatct aatcaaaatt gattgctttg ggtatgattt cttttaatat caatctatta
     3841 ctttaggatc tgaatcattt ttatgcaccg tatctaaagt gatattttct gtgacttcaa
     3901 taacataact aattctatca caacgaactg tggattgaga ataatgaaat ggttgattat
     3961 ttttatcttt gccaaccttt tctaaaataa atagcggatc tcctactata cattgcaata
     4021 agtcggaaag ctcaggtgtt gcagataaac cgtttatttc cataatagag ctcgtagctt
     4081 tgacatcata gtcatcttct agaagtttat ataatgaagt atattggcta cctttttcaa
     4141 ataaagttgg aaatttttct tctgatgcga aaaaattatc aattcccatc ggggcaccgt
     4201 cttcccacat taaacgttta atattgtaaa ctctatcagt tacattgatt tgtaatttat
     4261 tagctaaaaa ctcatcagcc tttataattt tagaatgtaa aacagtatat ctaattttat
     4321 gtcctcgggt agaaagagaa tcgctaaatc catgaactcc agctaatttt aaactttctt
     4381 ttttttgagt tacaaaggtc cctttccccc ttaatcgttc aactgttcca ctttctgcta
     4441 aaagtttaaa agcttttcga atggtaatct tacttactcc ataaatttta cataattctt
     4501 cttctgtagg taattgctct cctggaagat attttttttc acgaatatcg ttacgtatag
     4561 aagcttctac ttgtaaatat aatggtctat tatcttgaaa attcatagta cactccctaa
     4621 aaagttatac ttcattatat cacaatttat taaaacgtcg attgtaaaat taagctagac
     4681 aactaaaaag atcatttgtg ctacactcaa aatagttccg accaaagaat taataaggag
     4741 tgagtacaaa tgacttacac ccatcttaca acagacgaac tagtaatcat agagtcttat
     4801 ttcaatatcg agaaacctgt cgctcttgtc gcacaaagtt taaatcgctc tagacaaacc
     4861 atttataatg tttattcgtt tctcaaacaa ggaaaaaaag tgctagatta ttaccgtcaa
     4921 tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga tgaacaatca
     4981 acatatgttc aagaaaaggt agctcaagga tggtcacccg atgtgattat cggccgtggc
     5041 gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa aggccttttt
     5101 gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca agaaaagcgt
     5161 ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc acacctgact
     5221 aaagaatttg gtcatctcga agtcgacacc attgtgggag ttaaacacaa gagcgctgtt
     5281 attacattgg ttgagtgttt atccaaggtt attattactt tgaaaccgac aggcagacga
     5341 gccgaagata tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa acacttgttt
     5401 aaatccatga catttgattg tggaaaagaa ttttccaact ggcgatctat aagtaactcg
     5461 aacgatattg ccatcttttt cgcagatcca ggaacacctt cgcaaagagg gctaaatgag
     5521 cactcaaacg gcttgcttag acgagatggc ttacctaaaa caatggactt tagggacacg
     5581 gatgaaacct ttattcaatc cgttgcctcg aaaagaaacc atattccaag aaaatcatta
     5641 aattatcgaa caccattgga agtatttttg agctgcatag ataaagacat attgtctagc
     5701 ttaatttgac aaatcagata gtataaaatg aatcaaattg aatatcaaat atatattata
     5761 tccatgaagc ccaatcttct ttaatagaag attgggctat ttttatggca aaggagggaa
     5821 aagaaatgtt tcaaaataaa ggacaacgtt atgtcacaaa gggagtaatg gacagtttgc
     5881 cggtggaatt acaagcttta tgttggaatc taatcgatca aaacgttcaa aagcaatttc
     5941 atttagacta tcttcaaatc tttgaatttt ctacggaaaa aggaaatcaa aagttggttc
     6001 atcgtcaaga agaaccagaa gcaagaaaag agtatctgat ttcacctaag cttcgtttga
     6061 aatcagtgtc tcaaaaaata tgggtcattg attcgggtga gtatcaaacg atgttattac
     6121 cagaagaata ctgatatcta aaagaaagga acgaaaggac ttgttagaaa cggctatcga
     6181 agtactggaa aagtgtgcac agctagtcac tactcctgaa gagtggggct atgaatccgt
     6241 tacgatggaa aaagaggaaa ttgaaatggg tatgcttcct gaagatgtta ctctaccacg
     6301 tttagtgatg acacatcttt atatttattg cgcaccagaa gatggaaaag actatgtagt
     6361 ttatttcatt acagatatta ccagccagcg agaatttgtt cgtgggttac tggtagaagg
     6421 acgattggtt tggagtcaga ttggaggaac gaatgaataa gatcgattat ttgagaatga
     6481 ttgcgaaatg tttagaagat atccttacag cgattcatca aattctgaag aaaaaaggag
     6541 aaagaatatg aaatattttc aactagtatt gacacttata acggcaatgg tgattgaatt
     6601 gcttgaacaa aaattagcat aggagaaaaa gagatgattg aaaaagaatt agtgaccaca
     6661 caaacagaag tttggcgaga tcctcatcat acccatcgct atgttttcaa acgacaatgg
     6721 gcgatcaaag gaaaagaagc ggaagataaa ttagcggtag tcattactat tcgtccaaca
     6781 gatacagaac catttacgaa tgatttgagt atgttattaa tcgaaaagaa tatccgacaa
     6841 ttaggattta ctggttttat tgctgtgaat cttttttcgt atacaaaagc gaaaagtcct
     6901 cgggtatttc ctcgtggaaa tgatgaacaa tctttggaaa tattaacaac tgttttcaca
     6961 gaaaagaaaa tcaacaccat tatttttgct tgtggttcga ttacagcgac gagtcaagtg
     7021 gcttatgaac aagcgaagac catttacgat caattgagtg cgaaacaaaa aaagatgacc
     7081 aaagtattga taaatgcaga aggtaagtta tcccacccac taagtattca tgttcgtaat
     7141 gaatggcaat tggcagatag tcaattatta tttcaagaaa aataacattt tattttctta
     7201 tgttacttat aagtagtttt acatgctttt tgactttaaa aatacatgtg atgatacatg
     7261 ttatatgaat tatcaatgta cccagtaata tgctattttc attatgtaaa aaataacagg
     7321 gggtgtgtag atggagattg atgaaacttg tttgagtaac tcttataaaa gtatgaatga
     7381 cttttttgag gaaatggaac tggattcaga attgctctat caattttatc ttgcttttcg
     7441 agggcaacag atttcttttc caatgaaaat gtacgatcgt gagttagtta gaaaaagaat
     7501 cgaaaacatg atagaacagg aaaaaactgt agatatccgt caattgacag aagtttatgg
     7561 tttctctgca agatggattc aaaaagtaat taaacaaaaa gaaaggagac gagtacatgg
     7621 ctaagctgat tgtattaggt ggtttaattt acggtggata ttggttatat aaatatatgc
     7681 ttgcgcatcc gaatatagat aaaggaaaag taattaagac agttggggta atcatagttg
     7741 gactcatagc tctaactaag aaaaataata ctaatagccc aaaaataatg agaacttatc
     7801 gctgtagagg atgtggaagg gaaatttcta ctagttttaa tccatctgaa agccctcaag
     7861 gaagagatcg ttgtcatgct ggctatcgac attcttggga aaggattaac taaatgaaca
     7921 ccattgagat taattgactg ataaaataag tacgcgaggg agaaattgaa agggggaaat
     7981 aaaatgaaaa aatggttgtt attactaatt ggaatgggga ttttattcac tggttgtaca
     8041 tctgagaata cgaggactaa agagactgta gaaactgata caacaattga ggctaaaaaa
     8101 agtaaaacgc aatcaactga acaaactaca acagaaatga aacaatctgt ggaagcaagt
     8161 acaagtgaaa gttcaatcat tgaatcttct tctgaagagg ttaaaaatac tttgtgggat
     8221 agtgagaaat ctactcaact agcaattttt gtcccacaat ggggaaagac attaggtcaa
     8281 gaatataaaa gctacaatcc tcagaacaat gtaagtctgt atggtacgcc tttaccaagt
     8341 gcagttattg atggaaattg gaaaatggca atcaatgaag caccagttac cgtacaatgg
     8401 tcagaagatg gaactggtca tgcagatttc aatttagtag cagtttattc tgatgtagaa
     8461 actggagaat atttagggca gcatgtatat ttctttggct ttcaaaatgg acaacctaaa
     8521 gtttatttaa cgcaacaaaa tcaaggcaat gataaaaact acatgtactt caatgaaact
     8581 cagaaccaac agttaaaaca aggtttcatt gatattgtga atggacaaac tcctcaaact
     8641 ccagtagtag aagaatctac tcaacaagca acaactacaa cgacacaatc tacaagtagc
     8701 tcagcaattt caagttggga agctgctaaa gaaactgtac taaataataa agctttatgg
     8761 ttgtatgatt ctcctgataa aaatgcatca aatactaatt taattagcac aaatgaatat
     8821 atgaaagatc taatgcaaga tgaaaatgga agctactatt caattaatat tgtaacggat
     8881 gataatggaa atacaacaat ggctggtgga aaacaattcc gtgtttatac agatggaaga
     8941 atttctcaac gtattggaat gactaacaat tttcaaatga ttaaa
//
LOCUS       DACCXW010000113         8851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCXW010000113 DACCXW010000000
VERSION     DACCXW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.116"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            63..1277
                     /locus_tag="IVY71_002299"
                     /pseudo
     CDS             63..1277
                     /locus_tag="IVY71_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1791)
                     /locus_tag="IVY71_002300"
     CDS             complement(1456..1791)
                     /locus_tag="IVY71_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7986335.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="IVY71_002301"
     CDS             complement(1775..2047)
                     /locus_tag="IVY71_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP7986336.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="IVY71_002302"
     CDS             complement(2498..3301)
                     /locus_tag="IVY71_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7986337.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="IVY71_002303"
     CDS             complement(3279..4403)
                     /locus_tag="IVY71_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986338.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="IVY71_002304"
     CDS             complement(4419..5234)
                     /locus_tag="IVY71_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7986339.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6105)
                     /locus_tag="IVY71_002305"
     CDS             complement(5224..6105)
                     /locus_tag="IVY71_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7986340.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="IVY71_002306"
     CDS             complement(6181..7470)
                     /locus_tag="IVY71_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7986341.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="IVY71_002307"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="IVY71_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2742 a         1883 c         1410 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DACCXW010000114         8799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCXW010000114 DACCXW010000000
VERSION     DACCXW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.117"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..233)
                     /locus_tag="IVY71_002308"
     CDS             complement(<1..233)
                     /locus_tag="IVY71_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7986342.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEK"
     gene            505..849
                     /locus_tag="IVY71_002309"
     CDS             505..849
                     /locus_tag="IVY71_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7986343.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            850..1269
                     /locus_tag="IVY71_002310"
     CDS             850..1269
                     /locus_tag="IVY71_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7986344.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1361..1711)
                     /locus_tag="IVY71_002311"
     CDS             complement(1361..1711)
                     /locus_tag="IVY71_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986345.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1677..3008)
                     /locus_tag="IVY71_002312"
     CDS             complement(1677..3008)
                     /locus_tag="IVY71_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7986346.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3264..3830
                     /locus_tag="IVY71_002313"
     CDS             3264..3830
                     /locus_tag="IVY71_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7986347.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3975..4062
                     /locus_tag="IVY71_002314"
     tRNA            3975..4062
                     /locus_tag="IVY71_002314"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4007..4009,aa:Ser,seq:cga)
     gene            complement(4114..5319)
                     /locus_tag="IVY71_002315"
     CDS             complement(4114..5319)
                     /locus_tag="IVY71_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986348.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5457..6345)
                     /locus_tag="IVY71_002316"
                     /pseudo
     CDS             complement(5457..6345)
                     /locus_tag="IVY71_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(6432..6632)
                     /locus_tag="IVY71_002317"
     CDS             complement(6432..6632)
                     /locus_tag="IVY71_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986349.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6661..7083)
                     /locus_tag="IVY71_002318"
     CDS             complement(6661..7083)
                     /locus_tag="IVY71_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7986350.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            complement(7100..7522)
                     /locus_tag="IVY71_002319"
     CDS             complement(7100..7522)
                     /locus_tag="IVY71_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986351.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7813..8004
                     /locus_tag="IVY71_002320"
     CDS             7813..8004
                     /locus_tag="IVY71_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986352.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            8015..8281
                     /locus_tag="IVY71_002321"
     CDS             8015..8281
                     /locus_tag="IVY71_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986353.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
BASE COUNT         2853 a         1478 c         1590 g         2878 t
ORIGIN      
        1 tctttttcct cagcaggaaa aatgatttcg gttctcccac aacgtcgctt attttctttg
       61 tattgttcaa agtattcaag tgctgttcca ccacatttga gaaagttata gacattgtaa
      121 attgtttggc gtccacgttt aagctgcttc gcaacgatag caaccggagt ttcttgatga
      181 aaatatgctt ctatcattac aagctcgttt ggtgtaagat gggtataagt catttatgtt
      241 cactctcctt gtatgcttta gcgggtatta caatttgagt gtaacataaa tggctttttt
      301 atttgtctcg cttaattata caaacggcgt aaaataaaaa aagcgttagc aactatgctt
      361 aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat
      421 gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac
      481 agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta
      541 ggagattttg caccaaaatt tgcagaattg aatgatgatg ttttatttgg acaagtatgg
      601 tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca
      661 ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact
      721 agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtaggatg gccaaaagct
      781 tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa
      841 gaggcgtaaa tgacgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt
      901 gaaaaaaacg aagcttacgc tcaagtattt accgggcaaa gctatctgaa tatgttagtg
      961 aatgatcccg aagtttctgt tggtgtaggt aatgtaattt ttgaaccagg ttgtcgcaat
     1021 cactggcatg tccataaaga cggctatcaa atcttacttg tgaccggagg agaaggctgg
     1081 tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac
     1141 aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc
     1201 actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa
     1261 aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag
     1321 ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag
     1381 cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa
     1441 tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt
     1501 gatagtagat ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta
     1561 aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa
     1621 attctactcc aatacgtatt tcagttattt catgggaata taaataagta agaagctcat
     1681 cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta
     1741 aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta
     1801 tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagtaca
     1861 ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcgag acctgtccca
     1921 aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa
     1981 gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg
     2041 aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc
     2101 aaaacataag aagacagcat cagaccaact aagcctatgt tgatcaaagt agcaccaata
     2161 ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc
     2221 cccaaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca
     2281 aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt
     2341 gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat
     2401 ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga
     2461 agaagtagac caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga
     2521 aaacgcatac caaaagtgat ataactaacg accggtgata atgcgctacc aaacagtccg
     2581 atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa
     2641 atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga
     2701 atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa
     2761 agtaaggtca aaatccctac atttatgaat gcgcttccga aaccgcctaa agcaaaataa
     2821 tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta
     2881 ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa
     2941 tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg
     3001 ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg
     3061 gacaactatc atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat
     3121 ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaaatttaaa atataacgtt
     3181 ttgagtaaaa tgtttgcaaa aaatgctaaa aatagggtac aataaaacaa catatactgc
     3241 agtaaagaag tgggggagtt ttaatgagaa gaaaagtcta taccaaggat caaattctaa
     3301 aagctgctta tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg agaaatattg
     3361 ctaaaaagat ggggatttcg acacagccga tttatctgga attcaaaaac atggaagatt
     3421 taaaaaatac gttgctggat tctatctttg aacaactaaa agaagatgtt ttccctattg
     3481 aacgtactgg tgataaaatc attgatctcg gtttgaacta tatctacttt gcaaaaaagc
     3541 atcatagtct ttacatggca ttgtacattg atgaacatgg ggacggaaag aaaatgcatg
     3601 atttttcata caattatttc cgtgaaatag ttaaagaaga tgaaagatac aatcaattat
     3661 cggatgaata tgtcgatgct ctgcatactg gtacatgggt agcagtgact ggcttagcgt
     3721 cattgatgtc atcaaacatc atgcatccaa cagaagaaca aatcattgca ctgatcaaac
     3781 ggacaattga ctcgatttta gcattagata atcctgaaat aatcagatag tgttctatca
     3841 gtatgaattt gatcggtaag tccgttggct gcaaactgtc aatggacttt tttgctaaaa
     3901 aaagcgattt ctgttattta tctattagaa ataggcttga catttcgtga aaaaagttta
     3961 aaaatagtac ttacggaagg ttggcagagt ggtaatgcac cggactcgaa atccggcgaa
     4021 ccggctaata ccggcgcgca ggttcaaatc ctgtaccttc cttatatatc aacgattaga
     4081 cacgatttcg atcgtgtctt ttttgttgta tatttagcct aaaatgtttt ttgactcccc
     4141 ttttgactcc ccctgagtac tgttcttcat aaatgctaca aaattattga tgactttttt
     4201 gttttgagtt tcagttacat gagtgtaaat atttgaagta gtttgaatgt ctgcatgccc
     4261 taaacgttcc tgaacatctt ttaaagaagc tcctgattca aaaaggagag aggcatgagt
     4321 atgtcgaaaa ccatgagtag tgattctttt agtcaattct ggatgatgct tatatatgac
     4381 attaagccac gaacgtgaaa ctgtagggtt atagagaaga ccatcatggt gaaaaattaa
     4441 gttttccctt tggattgtaa cgatcgtttt gttttttgta taatactttc tcaataaact
     4501 gatcaaatca ttgtccagat aaatttttct ttttccagat tttgatttag gagtatttac
     4561 gatcaatctt ccttgataac ctcttgttac agttttattg atattgagtg ttttgctctt
     4621 aaagtcaata tccgaccagg tcagagcaaa agcttctcct tttcttatac ctgtaaaagc
     4681 caatagggaa aagaataaat atctttcatc atcatcgttt cttattgctt ctaaaaattt
     4741 gattaattct tctttagtat aaaattcgat atttttatct tcgatatcta gagcttctcc
     4801 acgtggaata gaaactaatt tcataggatt gctagttata atttgtaaag aagcagcgta
     4861 atcaaaaaca ttagatgtgt aattttttat tttcttaaaa attttaggat ggctatcgga
     4921 ccaagtgttt actgcttctt gacagaaaaa aacgtctatt ttgtcaatga acttatctcc
     4981 aaacactttt aagatgtgtg tgtcaaaaat ttgtttggtg gaggaccaag tgctttcttt
     5041 aactgtcttt ttataatttt caaaccataa gccgtaaaca tattcgaatt tcttgctctt
     5101 ttgagctgta gaagcttgca gtcctttttc ctgtatatct gcctccaatc tttttaaagc
     5161 tctctcagcg gctaatggcg tactaaaacc tcgtctcgtt gtttttcttt tttttcctgt
     5221 tagaggatca actcctaagt acagactaaa ctgatatttt tcttcgcctt tttttgttaa
     5281 gtgcttttta attcgtttat caatttcttt ttttgccatc tttatctttc ctttcgtacg
     5341 tttgttcggt tgtacagcgg atttcgagat ggtaaaatag ggtacaacaa atagacctac
     5401 tttaccgtag ctctttgcac atttgcgttc ttggtcgggc ggcaaatgtg ctttttttat
     5461 ttcactctta gttcttgtcc tggataaagc atgtagttgt ttggatccat accgtttaat
     5521 gcaaacagtt gatctaccgt aattccagct cgttcagcta tttgtttggt cccttcacca
     5581 ggttgcaaag tcaaggtttc ggtttcagat gaagaaggtg ccgttggttg agcttgatta
     5641 gctggtacat aatcttgtgg tactgattgc tccgttgaac tgggagtggg tgaaacttgt
     5701 tcaacacttg aactaggagt agcttctgca tagttttcta cactttcagc agagtaagta
     5761 ctactagttt caggaggcgt attctgcgca atagcagctt gttctacagt actagaagat
     5821 gcaatagtac tttctgttgt agtcgaagat gattgttcgt tgctacttgt tgagctagta
     5881 gttgttctag attctttagt ttttttcctt tttgtagggt ttcaaaacta atttctcatt
     5941 atcatcagat ttgttaggct tttctggagt taaaataata tttttatctt ctttgctgac
     6001 agtatatttg gcatcattat ctttgtcttt atcatctttc caagtcataa catcgccttc
     6061 aagtgtatac tcaaatttgt aattcatttg gtcgactaag cttttagcaa attcttctcc
     6121 catagcctcc caatcgttac ttgaagttga tttcatagaa cttgtgtcaa cactaaaaga
     6181 tacgacgtga tcagaaaatg atacgatcat attaggttcg tcatctttgg ttgcttcaat
     6241 caaccaatca ttagctttta aatcttctgt agttactttt ttaccacaag cagtgaaaag
     6301 taacagagaa gcaaaaacta ataaacttgt tattactttt ttcatttttt attcctcatt
     6361 tctatgatat gatttttatt ggggaatctt agaaataagg tttcgagtcc gtgttgctgc
     6421 acgggctttt tttatttaaa taaatcccaa aaactgaatg tagttttctt gtatacttta
     6481 ttgtaagcag ccttttttgg gtctttaatc cagccgctac cttttttccc ataaccagga
     6541 ataactgctt tttttacttt tctttttgct tttccagtgg ttctagcgct tatggatttt
     6601 tttatacttg gttttctcat tcctattttc atctatatcc tccttacgtt gtattataat
     6661 tcacaagaaa cgatcgaatt catctggtaa tccaaagcag acgagctttt catattttgt
     6721 catatgatca aagccgtcta aagttggatc aatagtcaga tgagttgcga aataatttgc
     6781 ttctttttca atttttaatt cagatactag cgttttttta gacaacatag gtgtgttttc
     6841 attcggatgc aaaacacagt ggcctaattc atgactacaa gttatccatt ttcccatctc
     6901 atctaaacta gaatttatgt ggatcatttt tatctgtttg aatttactat agtaaccata
     6961 aatttctccc aaatcttctt ctaaaactag tatcttcaat tctcgtgcaa ttctaaaagg
     7021 attgcgtgtt tgatatagtt ttaccagctt attaattttg ttatcaattt gaggtaaata
     7081 cataaacaac gtactccttt taatcgcgat attttttagg tgtgtatttt ttcttagctt
     7141 ctattttagc tattcgcaaa gcattctcta aagaaataat gagagcttca cgtgtcgctt
     7201 cactcatttc attatctttc ttcgaaaaag ccattccatc agcattagat aaatcgtcga
     7261 ttaatgactg caattctttt tggatatcac gctcgtcttt ttctgttaaa tcatagtatc
     7321 tttttttctc agtacgtcct agcaaataat ctacagatac gtcgaagtag tcagcaattt
     7381 cttgtaactt ttcggcagtt ggctgttttc ctgcgtttaa actataaaag taattctctc
     7441 cgtacttcaa atctttagcg acttgttgaa ggtttttatt cttgtttttc gctagttctt
     7501 ttattctttc aaatgttgtc attccaacgt tcctttccaa cacgaaaaaa taatttacga
     7561 aaaagagtaa aaatagcttt acaattttac gatatagagt tattatttat ccgtaagtta
     7621 atttgataga aaaaagcaaa ctaaaaacac accttatagc ataaagtttg gcgaccaagt
     7681 gcaaataaaa ggttttgtta taggcttatt tctctatgta ttcattttac tctatagtgt
     7741 aaaaatgtca atggaaatta tattttttct atcattttaa cttacaaatt tataaaaaaa
     7801 ggaagtgggt taatgtcaaa cttggataac ggtcgtgcgg cgatcaaaga gtttatgaaa
     7861 gagaatggaa taactatgga tgatttggct actgcttatg gtcacacgcg tgtacgaatg
     7921 caacaaattc ttgatggaca ctggagtgga ccaaaggcaa acagaactat tctagagatc
     7981 atccgtgatt acagaattcg ataggaggca agtcatgaat attctaagcg aagagttttt
     8041 gactcgttta agaattgcaa ttgttgaagt tgtaaaggac gcacttagtc aactttcaaa
     8101 aaagaatttg tcagaaacac gatatttaaa aaagatcgaa gctagaaaat atgtgggagg
     8161 tgtaaacgat caaggttttg agaagttaat agctcacggt ttaaaagaaa ttcgtataga
     8221 tggcttttta agatatgaca aaaaggacat cgatgaactg atggctaaat acaaaattta
     8281 aaggaggtca acatgggaaa gtttaatcga acattagtat tcagcgcgcc actaattatc
     8341 tacgctttag gtctatgggg tagtagacaa gcattaatcg gcacgatcgt ttacatgctt
     8401 tggattttca ttgggcttga tgaagctgag tacagagcga aaaagccagt cgggagggac
     8461 tgactaaaaa agtctaatgt ctataagaaa ggtttgacaa taaatgaaag aattattttt
     8521 tttaataact ttcatcattt tttctatcag tgttttctac attaaccttt gcgttgctag
     8581 atttgctttg aaaagtttta ggaacagcaa acgaaaatag agctataaaa gatacgaatg
     8641 tagtagctac gttaaattgc tctttagaaa acgtcaatgt cattggaatg ataattgata
     8701 tcgatgagaa aatctttgcg ataataacta tccatttttc aattataaac atttttaaat
     8761 taagcaccat tagacaaaat gtagttccta tatagagga
//
LOCUS       DACCXW010000115         8730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCXW010000115 DACCXW010000000
VERSION     DACCXW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.118"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            419..865
                     /locus_tag="IVY71_002322"
     CDS             419..865
                     /locus_tag="IVY71_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7986354.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            1489..2481
                     /locus_tag="IVY71_002323"
     CDS             1489..2481
                     /locus_tag="IVY71_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7986355.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            2497..3381
                     /locus_tag="IVY71_002324"
     CDS             2497..3381
                     /locus_tag="IVY71_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7986356.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            3430..5037
                     /locus_tag="IVY71_002325"
     CDS             3430..5037
                     /locus_tag="IVY71_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7986357.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            5038..6522
                     /locus_tag="IVY71_002326"
     CDS             5038..6522
                     /locus_tag="IVY71_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7986358.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            6699..7691
                     /locus_tag="IVY71_002327"
     CDS             6699..7691
                     /locus_tag="IVY71_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7986359.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            7743..8612
                     /locus_tag="IVY71_002328"
     CDS             7743..8612
                     /locus_tag="IVY71_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7986360.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         2819 a         1365 c         1905 g         2641 t
ORIGIN      
        1 tatgtttggt ttagtacaac ctaataaagt gaaggtgggc caacgaatca aagaaataaa
       61 agacggaatg tctctctctt ttacagaatt agggaatcga ttaggtttaa aaaagccgac
      121 gatcagttcc tacgttcaag gctatgcgtt ggctcctaag agtgtgatca accaattatc
      181 tagtatcagc ggaaaacctg tcggctggtt ttattttggg catgtggaag aatatatccg
      241 agagtatttg tttctcattg gtcagaaaaa aatagtggac gattatcctg aaatcattga
      301 gaaaataaag caagaatttt atacgggcga gttcaaaaat cctgcttggg agaatgagct
      361 aggctatcct ttggaggaat ttatccaaga atgttttgta gaatattcga gtgaaatcat
      421 gaaggactat gtcgtacaga ttacgaaaga ggtacttgaa gaatccaaaa cgctaaacaa
      481 actatcatca aaagcaaaag aagaagctgt cacgattctt tcatctggga tcattgacta
      541 tatcgagatg agtggtgaga tcaagtatgg agagaaagat cgcattgcga aaatggtgaa
      601 agaatcaata aaaacctttg atgtgaaaca agacctgaca tttaatgatt cctatttgat
      661 tggaaagttg atcaatattc ttggtacaga tgaagatacg gagctgtttc tttcttcttt
      721 atcaatgata tttactgata agcatttttc cactcgattt ggtggggaag aattagtcga
      781 aatatttcaa tcgatgcgac cagcgttaat caaactctat cgggagaaaa caccagatga
      841 attttatgat tggtttgaga agtaaaataa gaaaagtggc ttagattaca agttggtgaa
      901 aaaatttttt tcagcataac atcagtaatc tataaagggg ttgttgatta tactgtagaa
      961 gttaagtact caagagattt tttgtacgta aacaaagcaa aaaagggtgc agtttcacta
     1021 gaaaatgtac tcttttttgt gatttttctt agttatagag tttttttgaa actttaaaaa
     1081 tgagaaaaca tattgatttt aaagagtgta gacatagctt cttttgctaa atagattaaa
     1141 cttactggtg agtggtcttg aacatacttt taaatcacat tttactgagt tttattggat
     1201 tttataattc attggaaacc tcgtacttaa tctgttttta tctatcgact agaatctgta
     1261 tatattccag ctatcaatga ggccgcagaa aagggtgctt aaaaataatc tgttgtttaa
     1321 aatattatta tcagaatact tagatacaaa attctggcac aaagtaaaaa aaatattgac
     1381 ttaaaaaaat ggatatggta atctataaac gaaaatagtg gtaacgtttc caaaatgcat
     1441 cttcgttaaa tttttatttt tttaaacgaa aatggaaacg tttctaaaat gtattgggag
     1501 gagattgaat tggaaaagac attgggacca ggtggtgaga taagggcggt acaacgggat
     1561 aaaaaaaata tttcgaagtg ggaaaaatta aagaaacagt tgcggcgtga taagtggatg
     1621 tacttgttgc tcttaccagg tatcatcatg acgtttatct ttaagtatgt gccaatgtac
     1681 ggtgctgtga ttgcatttaa agactataat cctatgctag gaattttgga aagtccttgg
     1741 gtaggactag atcactttag ggaatttatc agttcaccaa attttacgct tttgctgagc
     1801 aacacattaa aaattagtat ttatggttta ttgttaggtt tttttccacc aattatttta
     1861 gcactgagct tgaatcaaat tatcaataaa aaaataaaac agaaaatcca gttgattcta
     1921 tatgcgccga attttatctc agtagttatt attgtgggga tgctgttttt gttcctatcc
     1981 gcaaatggac ctattaatgg gttagtagaa aaggttacgg ggcaaccaat tatgttcatg
     2041 tctaatccag cttatttcag aacgatttat attttatcgg gaatttggca aggaatcggc
     2101 tggtcatcca ttctttacac cgcaacgctg gcgggagtca gtaccgattt atatgaggca
     2161 gcaaatatcg atggggcaaa tattttacaa aagatttggc atatcgattt gcctgcattg
     2221 aggcctgtga tggtgatcag cttcatcttg gccgccggcg gtattatgaa tgtaggatat
     2281 gaaaaagcct acttgatgca aacttcaatg aacatccctg cctcagaaat catttccact
     2341 tatgtataca aagtcggctt gcaatcaggt gattatgcct actctgctgc tgttggtctg
     2401 ttcaatatga ttattaatgt cgttttatta ctgattgtca acaatataac caagcggcta
     2461 aacgatggtc aaggcttgta atgaaaggag aaaattatga atagtgcttt atcaaaaatc
     2521 gaccgtcaaa tcttatggtt gaatcgagtg attgtaactt ttttagtcgt catcaccatt
     2581 gttccgctga tttatgtact ggtagcttct tttatggacc cagctacttt gatcaataaa
     2641 ggtataagct tcaatccgaa agattggaca atacagggat atcaacgggt atttgcagat
     2701 gattccattt tacgcggttt tgtaaattcg ttgttttact cttttagctt cagcttattg
     2761 actgtattta ttacgatgat cactgcgtat cctttgtctc gacctaactt ggcggggaaa
     2821 aagattatta tgactttttt tgtcattaca atgttcgtgg gtggtggctt gatacctacc
     2881 tatttattgg taaagaactt gggcatgtta gatagtgtat gggcgattat cgtaccaggt
     2941 gctataaatg tgtggaatat tatcttagcc cgtacctatt ttagtgcttt gccaaaagaa
     3001 ctttctgaag ctgctactat cgatggcgcc aatgacttgc aaatactgtt caaagtcatg
     3061 cttccattag cgaaaccaat tatgttcgta ttgtttttat atgcctttgt tgggcaatgg
     3121 aactcatact ttgatgccat gatttacatc aaagatcccg acttacagcc gttgcaactc
     3181 gtattaagaa atattttgat ccaaaaccaa ccttcacaag acatggtggg agccaatact
     3241 gccatggcag aaatgaaaca aattgctgag ttgatcaaat acgcaacgat cgtcatttct
     3301 agtctgccat taatcataat gtatcctttc ttccaaaaat attttgataa aggtgtttta
     3361 gttggttctg ttaaagggta agtattgttc actatttgta tgaataaaaa ttataaagga
     3421 gattttaaga tgaaagcaaa atggatgctg ccaatcgttt ttacttccgt actaattttg
     3481 ggggcttgtg gaggctcaaa agaaaagagt aaatcaagcc ctgattatga actagaaaat
     3541 gtctcttttc cattgaaaga aaaagtatct ttaaaaatga tgtctcaaag tgcccctttg
     3601 gctcccaatg atcctaatga caaattgatt ttccaacgat tagaagaaga gaccaatctc
     3661 catattgatt ggacaaacta cagtagtgat tttgccgaaa aaagaaattt ggatatcgct
     3721 agtggtgatt taccagatgc catcttcaac gccgctgcag gtgactatga tttattaaac
     3781 tgggcggaaa gtggcgttat tgtaccgatt gaagatttga tcgaagatta tatgccaaat
     3841 ctgaagaaaa tttttgatga aaaccccgaa taccgacaaa tgtctactgc acctgatggt
     3901 cacatttact ctatgccttg gattgaagaa ttaggcgaag gaaaagagtc gattcataca
     3961 gtaaatggga tggcttggat caacaaggag tggttggata atttgggtct tgaaatgccg
     4021 caaaccactg atcagttgat gactgtgttg gaggctttca agacgcaaga tccaaatggt
     4081 aatggtgaag cagatgaaat tccttttagc tttattaaca atggaggaaa cgaagactta
     4141 aaatttttaa tgggggcatt cggtctaggt gataatgatg accacttagt cgttgataac
     4201 gatggtcagg tacaatttac tgctaacaaa gaagggtata aagaagccat caaatatttc
     4261 aatgaaatga ataataaagg tcttattgat aaagaggctt tcgaacaaga ttggaatacg
     4321 tatgttgcca aagggaaaga acataagtat ggcgtgtact ttacttggga taaagcaaat
     4381 atcacgggtg cagatgatag ttatgacgtc ttgccagtct tagcgggacc agatggaacg
     4441 aaacatgtta ctcgtacgaa tggcatgggt ttctcaagag atcgtttcgt aattactagt
     4501 gcgaataaaa acttggaact tacagctagg tggatagata aaatgtatga accgttacag
     4561 tctgtccaaa ataactgggg aacctacggt gatgaagcac aggcgaatat ttttgaatta
     4621 aaggatggta tgttaaaaca tttgccttta gaggggacag caccggggga actaagacaa
     4681 aaaaccgaag ctggaggacc attagcagtt cttgattctt attacggcac agtcactact
     4741 atgccagatg atgcgaagtg gcgtttagat ttgatgcatg acacgtattt gccatatatt
     4801 acagaagaca atatctatcc acgtgtcttt ttagaaaaag aagaaacaga tcgattagca
     4861 aaaatcgaaa cggatatgtg ggattacatc tatcgcaaaa gagcagagtg gatcaccggc
     4921 ggtaaaatcg atgaagaatg ggaagaatac cttgctgaat tggaacgtgt aggtttggaa
     4981 gaatggattc aaataaaaca aactggctat gataattttt tgaagggagc acaataagtg
     5041 ttagtagaag aaacggcgat tttggataaa gcaaatcgat ttattgaaat gatgcgagga
     5101 aaagttaacc ctcagtatcg acaaaactat catttttccg cacccatcgg gtggataaat
     5161 gaccccaatg ggtttatata ttacaaaggg cattaccatc tcttctatca atactatcct
     5221 tacgatagtc gatgggggcc catgcattgg gggcatgcgc gcagtagtga tttggtccat
     5281 tgggaagatc taccagtagc gttagcccca gatcaacctt atgacagcgg cgggtgtttc
     5341 tcaggaagtg caattgaaag agacggaaaa ctatatttat tttatacagg ctgtcaagaa
     5401 attgatggta ttagccatca gcatcaatgt ttggcggtct ctgaagatgg tattcatttt
     5461 agcaaataca gtggtaatcc tgtggtagca gaaaaacagc taggtgaaaa tgggctaata
     5521 caagaatttc gtgatcctaa agtaattgtt cacaacgatg gttactatat ggtagtagct
     5581 actaaatcta cacagtctac tggtagaata gtgatgtttc aatcgtcaga tttgtatact
     5641 tggcactttt actctgtgct gctagaaggg acttcagagc aaggaataat gtgggaatgt
     5701 cctgatttat tccatttaga cggacgagat gtcttaatca tgtctccgat acaaatcaag
     5761 aagaacggac tggaatatca taatatcagt tctaccatgg cttgtattgg tgaggtaaac
     5821 tgggaaacag gtcagatgat agttgataat ttccatgaaa tggattttgg aatggatttt
     5881 tatgcaccac aaacaacaag agatcctaat ggtggacggg tgatgattgc ttggatgcag
     5941 atgtgggatc gtacattgcc tacacatgag ttaggccatc tttgggcagg ctctatgagt
     6001 ttgccccgag agttaagagt gaaaaaaaat cgattggtac aaaaacccat ctcaactgta
     6061 tatagtcatt tagtttatca acaaggcagt gaaaatattg cagtgactga cacatcagtc
     6121 gtatttcgaa acgcgctgtc tgattcttca tatttacatc tagtcgcaga tttggagaaa
     6181 gctgaagttt ttacaattga attactgcgg tctaagacgg attgtctcaa actagcctat
     6241 gacactgcta cacaggtttt tgaattggat cgtcagaaac taggaagaga gcttacaggg
     6301 gcggaagaag accctctgaa tgtacgaaaa gttgaagtac ctttgattga taatcagctc
     6361 gttcttgaga tttttagaga tacctcatca attgaagtct ttattaatgg gttagaagtt
     6421 atgacggcta ctttctatga gctggaaaaa gctcgagata tcttgtttca ttcgatcgga
     6481 gaaagtcgta ttcaagcttt tgaaacagcg agtgtgaaat aaatgtttga ttgcttaggc
     6541 gatgaatcaa ttatttaata ggttttgaag gataatgttt ttagaaaaga acagatagct
     6601 agatttccca tggatttgct attagtattt tgaaaaaatt agtaatttat gacaaaattc
     6661 attatactaa acttgtgcag aaatgtgagg gatcaactat gaaagcaaca atgaaagatg
     6721 tcgcaaaatt agcaggtgtt ggagtaggaa ctgtttcccg ggtattgaat aacggttctg
     6781 ttaaagcttc tactagagaa aaagttgaag cagcaatgac agagttaaac tatcaaccag
     6841 atttttatgc ccgggggtta aagatgaagc atacgaacac ggttgctttg attctaccca
     6901 cggtatggca tccatttttt ggcgaatttg cctaccatgt agaaacagca ttagaaagac
     6961 aaaactacaa gctttatttg tgtaactcca gtggtaatag tgaaaaagaa caagaatata
     7021 tccagatggt cagccaaaat aaagtagacg gcatcattgg aattacttat tcagatatcg
     7081 atgaatacat ttcgagtaat ctgccttttg ttagtattga ccgttatttc actgaagagg
     7141 tgatttatgt gaccgctgac aatgcggccg gtggacgttt ggctgcaaat gaactttatc
     7201 gccgaggatg tcagaatgta gcatatatcg gtgggtacca agaaacaccg aatgaaacga
     7261 agagtcgtcg aaaatatttt gaaatcgagt gccgtcaact ggggatttcc tataaagcgt
     7321 tagatatgtt agaaccttta gaccatctct cagaacgtgt aacaaatttt ttgttagaga
     7381 atcccgatat cgatgggatt tttacgatca acgatccaat ggcattagct gtcattgata
     7441 tgcttgaaaa aatagggaaa aacgtaccag aggatgtcca agttatcgga tttgatggtc
     7501 aaaagatgac agaaggagat ccttatctag tctcaacgat tgcccaacct acaaagcaaa
     7561 tggcggaagt ggcagtggca aaattaattg ctgtcattaa tggagagaca gtagaaaaac
     7621 gtaccgtatt gccgattgaa tttggcgaag gtgacacaac gaaaccttta gtgagaaacg
     7681 attttgctta gtgcaaatac gtttcttttt ttgaaaatga gtgaatgaaa ggtgtagata
     7741 aaatgttata tggtggaata gaagccggtg gaacaaagtt tgtctgtggg attggtaatg
     7801 aacaattaca agtgatagaa cggaaaagtt ttcctaccac aacaccagaa gaaacgatgg
     7861 agcaagtgtt tgatttcttt gataagtaca aaggaaatat cgcatcaatt ggtgtaggag
     7921 catttgggcc tattgatatt caaatcgaat caagaactta cggatatatc accaacacac
     7981 cgaagttagc ttggcaaaat tatcattttg taggagcttt ggaaaacgcc ttgtcggttc
     8041 ccatctattg gactaccgat gtaaatgctg cttgttatgg agaatatatg gcagggtatg
     8101 gaaaaggtaa aaatagtgtt ctgtattaca ccgttggtac aggtgttggt ggtggtgctg
     8161 tagtggatgg gaaaatagtc gcaggtttta gtcatcctga aatgggacat atgctcttac
     8221 aacgtcataa tgaagatcaa tttgaaggaa tctgtccttt tcatcatgat tgtttggaag
     8281 ggttagcatc aggacctgcg attgaaaaac gctttggaag aaaagcccag actatctcat
     8341 ttgatcatcc ttattggacg attgaggcag attatcttgc tcagtgtgtg tataacacaa
     8401 cgttactata tgcaccggaa gtaataattt taggcggggg tgtaatgaaa cagccgcaac
     8461 tactcgagtt gattcgccaa tcgtttaaag aaaaaatggg ttcctacgtg gatacgccta
     8521 ctctcgaaga atatctacta actccaaggc ttgctgacaa tgcgggcttg attggctgcc
     8581 tagctttagc aataaagaaa ctcaatcatt agaatagtat ttattagaac tattcataac
     8641 ataccggaag tttaaaaggg tattctgaaa cttaaatagt ctcaattcat tcttctttct
     8701 tgagaatctg agtagagact tttattctgt
//
LOCUS       DACCXW010000116         8630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCXW010000116 DACCXW010000000
VERSION     DACCXW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.119"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(228..2609)
                     /locus_tag="IVY71_002329"
     CDS             complement(228..2609)
                     /locus_tag="IVY71_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7986361.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2599..3246)
                     /gene="recU"
                     /locus_tag="IVY71_002330"
     CDS             complement(2599..3246)
                     /gene="recU"
                     /locus_tag="IVY71_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7986362.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3310..3849
                     /locus_tag="IVY71_002331"
     CDS             3310..3849
                     /locus_tag="IVY71_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7986363.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3939..4358
                     /gene="gpsB"
                     /locus_tag="IVY71_002332"
     CDS             3939..4358
                     /gene="gpsB"
                     /locus_tag="IVY71_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7986364.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4380..4747
                     /gene="rnpB"
                     /locus_tag="IVY71_002333"
     ncRNA           4380..4747
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVY71_002333"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4956..6122
                     /locus_tag="IVY71_002334"
     CDS             4956..6122
                     /locus_tag="IVY71_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7986365.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6168..7664
                     /locus_tag="IVY71_002335"
     CDS             6168..7664
                     /locus_tag="IVY71_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7986366.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7745..8428
                     /locus_tag="IVY71_002336"
     CDS             7745..8428
                     /locus_tag="IVY71_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7986367.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2710 a         1568 c         1833 g         2519 t
ORIGIN      
        1 gtatttagcc gatttaatta taaacaagta aatggataaa gtatatgtaa tgataaaaga
       61 aaaatggatt ttctaatttt taagctaaat gttagatcac acatgtatat gctggatagt
      121 cttataaaaa atcatgtaat acagcttggt acatgatttt tatttgtagc tgttttttac
      181 atagacacaa aaaagaaacc acgcttgtag acgtggtttc ttttttacta tctgtttcca
      241 gaagctgagg tgctgctgga tggtgtatgg tcatttgcag catccgctcc gctagttgct
      301 ggctcactgg aagaagcagg aggagcagat gaagacggtg tcgagctttc ttcggatgaa
      361 gaagaaggaa ccgttgttga agacgctggc tcgcttgttt ctgctgtcgt gctttctttt
      421 gaggactcag cagttgattc gctttgacta gtagagctag agctttgagt cgttgtctct
      481 gttgttgagc ttccaggagt ctggacatag ctggaagatg gaatagttgt tgatggtgta
      541 attgcattcg atctagctgt atattgatct ttgtagtaca gttcaccgcc cacacgaatt
      601 aatccgcttg gcatctccca gtctgtattt gtcacgttag ctgaaacata ctgcataagc
      661 tcgcgataaa catcagaggc tacgtgtgac gattctgaag tgacaggcgt cattttttta
      721 ttgtaacctg tccacacgga aatcgaataa tttggcgtat agcccgcaaa taggatatct
      781 ggatacaccc cagaagaaat accaagtttt gcatactcgt catctgtgta attggatgtt
      841 ccggtcttac cggcttgata taacccagcg atttgcgcat tcgtaccagt tccttcagtg
      901 atcgtatctt ttaaaatgtc tgtgatcata taagctgttt ctggagacat ggctgttttt
      961 ccatctggct cataagtttc ttctgttccg tcttggaaga cgattttatt aacatattga
     1021 ggtttgtaat aagtccctcc attggcgaaa gccgcatagg ctgctgccat ttttaaggaa
     1081 gaagctccgt attttgtccc atcttgttct tccgtattac tagagatcgc attcgattga
     1141 tggatcgtac tgtattcgat cccaagattt tttaagaaag aagccacttt atctgaaccg
     1201 acttcattga ataatttgac tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa
     1261 gtaatggtgc ccatatattg attatcccag tttcctacag gagtcgaagt cccttcatag
     1321 ttatacggtg cgtcagtaat agtttttcca gtagaatatt tcaaatattc aaaagctggt
     1381 ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac gggaagtatt cactgctaag
     1441 ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc caatctgggc ttttactttc
     1501 ccggtattgg tgtcgataag ggtggaggca acttgcatct cgtcgtctgg gtaagagaca
     1561 tactgatccg tgttgacgat atcatatagt ttcttttggg cgtccagatc caaattcgta
     1621 taaatctcta aaccgtctgt gtagacattc ttatctgttt tttcttgtac ttcattgatg
     1681 acttctttga cataattatc gacgatctta gtattgtcat cggattgagt tagttcttgc
     1741 agaccatccg ttaccggtac attaacagct tgatcatatt ctgtttgaga gattttttcg
     1801 ttttgaagca tcgtatacaa aacagtatct cgtcttttct tcgcctgatc tggataaaca
     1861 taaggatcat aggcggaagg ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa
     1921 gaaagttctg ataatttctt accaaagtac atctcagaag ctgtctccat cccgtacaag
     1981 ccgtttgaca tatacacttt atttacgtag taggtcaaga tctcttgttt cgattttttt
     2041 tgttccaatc ttacagccat ccacgcttct tgggccttgc gtttcagagt ctgatcctcg
     2101 gcactggtgg agaagaagga aagtttgatc aattgctgtg tcaaagtact tccgccttgc
     2161 agtccgccag aggtaaagtt agagagggca gaaccgatga tccgaatagg gtcaaccccg
     2221 atgtgtttat aaaaacggcg atcttctacc gacacgatcg catcttctaa tgttttcggt
     2281 aattcattcg cagaaatttt ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca
     2341 tcttgtgtat atagtttgga agatacggta gcgtctaatt ttttatccgt caattcgggc
     2401 gcatcttttg cgtaatacca gaaaagtcct acacctgcta aaaagaggat acagaaaaat
     2461 gagagtattc ctaaaaggat cttgataaat aatccttttt tatgtgttcc agaggacttt
     2521 ccagaacctt tgccagaatt cttcttcaca ctctttttag gagtaggctg tttctgtcgt
     2581 ctagagcttc tagtttgttc atttgccatt cttgctttct ccttcaatag ataatgttga
     2641 taaatagaag tcaaccgcgt ccaaataagg aatccgcgga gaaataccag ttttgatctc
     2701 tattcctagt tcttcgatca aagataaagg tagtgatttg cgtccttttt ccttttgggc
     2761 gtcccagtaa gcaatcaaat gaacactatt caaataaaaa cagcgcttca aagacgaaaa
     2821 ccataacaag acaaagcaga ccccattttg ttcgaggcat gctttcatat gttcgatttg
     2881 atgggcatgg aagtttttaa agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa
     2941 gtccaagtaa aagcctcgat agacgccatt atagtctgta gttgaggctt gggtaaagta
     3001 ggcttccttg atcacagcag cacttctctt aggatagtct actttcacga tttggatagg
     3061 ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga taatatttat tactttcatt
     3121 gattgcttct tcaaagcgca tcccccgatt gccgaattca gtgatctgat ttcttttttc
     3181 ttgaattttt tcgacgggag aggcattctt ataataaggt tggccatttg gatagcgcaa
     3241 taccatcttt aatcacactc ctaggccaca ttatagcaaa aaagcggata gaaagaaaag
     3301 gaatctgaga tggataagtc aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc
     3361 ggtgttttta aagaaaatga tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa
     3421 ctccatcagt tagcagaaga agggctggaa tgggtacttg tcagtgggaa tttgggcgtc
     3481 gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag aatatccgga actatctctt
     3541 ggtattttgt atcctttttc tgattttggt aataactgga atgaagggaa ccaagaaaaa
     3601 aaacaattag cagaacaatt agcagattat gtcgaagctg ttagccatca accctaccaa
     3661 tcacctagtc agctaaagaa tcacactcgt tttatacttg agcatacagt gggctgtctg
     3721 ctgatctatg atgaagaatt ccctggaaaa acgaagtatt tttttgaaga tgcacagaat
     3781 taccaagcag atcatcccta cgaaattcgg ctgatcagta tggacgatct tcaaaatttt
     3841 agtgaataat cgatattgtg tttgatttat caactttttt cctatacaat gaaaaaggta
     3901 gaaatgttat tacgtaaatg aaaactagag gtgtaaatat ggcgaatttg gtttatagtc
     3961 ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg cggctatgat cccgttgaag
     4021 ttgatgaatt cttagacaat atcatcaaag attatgaaac ttacagcaaa gaactccttg
     4081 ctttacaaga agaaaacgat cgattgagtg caaaagtagc tcaattatct aaaactcaag
     4141 gagcagctca aacacgcgtg caacaaacag aggcaccaaa gagcgcagca gtgaccaatt
     4201 ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc
     4261 aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa taactacaca aatgcagaca
     4321 caagcttaga tgataatgaa aaaacacgtc aattttaatt tttgattaat gaatttgcgg
     4381 ttttcgggta atcgcggtct gacttgatca ggctgaggaa agtccatgct cgcacaggct
     4441 gtgatgcctg tagtgttcgt gcttagcgaa accataagct aaggtactct ttggagtaac
     4501 ggcaggaaaa caagctaagg cttcggctat gcttaagtat ccttgaaagt gccacagtga
     4561 cgaagcggct ggggaaaccc atccggtgga acgcggtaaa cccctcgagc gagcaaccca
     4621 aacaatggta ggggcgcttt ttgtctggaa gaagaacagg acaaaaaggc agtataactg
     4681 cagatagatg gttaccgctt tgttttttct ccctggaaaa gcaaagtaca gaacatggct
     4741 tatagaaaac cgcaaacatc agggaattag gtcgtgacaa aagtaatgcg ttataagaaa
     4801 caagaaaaac atttgaaagc tatttttcaa tacttcagat gaatcgttct tattacaaaa
     4861 caaacttttg tatcaccggt tattcggaat ttgagtaaaa gtgtggggta ggactgatgt
     4921 cccacctcct ttttttataa ataaggagat tgtgtatgga aaaaaacagg aagttcaacc
     4981 tcgtcgcaac tgcggcaagc gggctagaag cactggttgg taaagaatta agagatcttg
     5041 gaatcgaatg ccaagttgag aacggacgtg cacgttttga aggaacaatg gaaacgatcg
     5101 ctacagctaa tttatggtta cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg
     5161 atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc attgccttgg gaagattttc
     5221 ttccactaga tgcagctttt cctgtagctg gaaaatcgat caagtcgaaa ctatatagca
     5281 cacctgattg ccaagcgatc acaaaaaaag caatcgttga acgtctccgt acctattatc
     5341 accgtccagc ttctgtacca ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt
     5401 tgctgaaaga ccatgtgatg gtaactctgg atacgacagg accaagttta ttcaaacgtg
     5461 gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa catggcagca gcacttatta
     5521 tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc
     5581 tttgtattga agcagcctta ataggccata atatcgcacc aggattcaat cgtgagtttg
     5641 cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga aaatgttcga tcagcagccg
     5701 aagaaaaagc agattatgat attgaattag atattactgg ttcagatatc aatggacgga
     5761 tgattgaaat tgccaaagcc aatgcagaag aaatcggatt aggctcttcg attaccttta
     5821 agcaacaagc agtcaaagac ttcaaaacgg ataaagaata cggggtcatc gtggctaatc
     5881 cgccttacgg agaacgtcta ggagaagaag aaactgttcg gaaattatat aaagaaatgg
     5941 gcgatgtatt ccgtccatta aaaacatgga gtaaatatat cttgactagc gacttagcgt
     6001 ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc
     6061 gtacggatct attccagtat tggggaactc gtccgcctag aaaaaaagag aaagacgaat
     6121 aatcttacta attgattcat tcaattggat gtttggagga atagggaatg aaagaaaaag
     6181 agtttttaca agaagtaaaa gaaatcaatc ttctacaaca agctttagga atcctcgatt
     6241 gggatacaca aacaggtatg cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct
     6301 atctttacag catttatttt tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct
     6361 atttttctga acatccggaa gaattgtcgg aagttggaac tgccgttttt gccaaagtaa
     6421 aagaagagta tgatttggaa catcgcgtac cggaagatct tatgagaaaa tatacagctg
     6481 ccgtttcttc agcacacgga caatggcaaa aagctagaga aacacaacaa ttttctgatt
     6541 ttcaacacgc attgaatcaa aatattgaat taacaaaaca gttgatacct tactggagaa
     6601 aagatgaaaa gacgaattat gatgttttgc tgaatcaata cgaacccggg atgactgtcg
     6661 aaatccttga taaggtattt gatcagctta aagaaggaat tctttcgatc cgccgtacgc
     6721 tccaagaaaa agggactgaa cctgaaacag actttttaaa tcggagaatg acgaaaaaac
     6781 aacagaagag atttgtagtg aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc
     6841 gtttagatga cactgtccac ccatttatga ctggaattaa tccgaatgat gcacgaatca
     6901 cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt tggagtgatc cacgaggctg
     6961 gtcatggtat gtatgaacaa gacatcgatg ataaatatgc gtatacaccg gtttatgaag
     7021 gtacatcaat gggtatccat gaatcacaat cgttgtttaa cgagatcatt attggaagta
     7081 gtcgcagctt ctggaaaaag caatatcctt tctttcaaga atgtgcagaa ggaacattcg
     7141 atgatattcc ttttgacaaa ttttatcgtt cattgaacca tacaaaatca agtttggttc
     7201 gtatcgaggc agacagtttg acttaccctc tccatatcat cattcgttat gaaatcgaaa
     7261 aaatgatttt caatgaaggg gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat
     7321 atgaagagta tctgggaata cgaccaacaa atgatgtgga aggcatccta caagacgttc
     7381 attggtcagg tgcaagcttt ggttattttc cctcttatgc attagggtac atgtatgcgg
     7441 cacaattact tcattctttg cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg
     7501 attattctcc aatcaaaaac tggatgtcag agcatatcca tcaatatggt gcatctagaa
     7561 aaccaaatca attaatcatg gatgcaacac atgaaccgct taatccgcaa tacctgatcg
     7621 actatatgaa agacctgtat tttaatgtat atcaggtaaa ataaaataca atttacaaag
     7681 atagctgaaa ttcatctaat ctatagatgt tttcagctat ttacttaaga aagaagtgga
     7741 acgaatggat aaattacaag tgatcgctga ttatagtatg gaccaattag cgcatgatca
     7801 aacaggacat gggagcgatc ataccaaaag agttgtcaag ttagctgaaa aaattttgga
     7861 gacggaaccg caggcagacc gctttgtgac gcttgcagca gcttacctac atgatacgat
     7921 tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa cagcttcgtg tattccttcg
     7981 ctctttgcca atagtagagg aacagatctc gatgattttt tccattatcg aaaatatgtc
     8041 cttttctaaa aatctctctg aagcggccga actatctctg gaaggtaaga tcgttcaaga
     8101 cgctgatcgt atcgaagcat taggagcaat cggcattttg cgtactgctt atttcggcgg
     8161 tggacatggg cacccgattt ttgatcctga actgtatccg caaacattca aggacaaaga
     8221 aaattaccga aaaggcacga cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc
     8281 tgataaaatg aatactgatt acggatatga agaagccaaa cgaagagaaa ccttcatgcg
     8341 tgaattctta gaagaatttt ttctggaatg gtctattgac gatgaaaatt ttactccaga
     8401 caattggatc aaaaataaca aaaaatgatg gaggttgtga cagaagtggt cagctccaag
     8461 atatttcgga tcattttccg taaaagttac ttctggaaca ctgtttacca agaggtcgtg
     8521 agcctgccgt tcagctccga gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg
     8581 atgaatattg taacttgtga ttcggaattg gcaggagaac accgtttatt
//
LOCUS       DACCXW010000117         8605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCXW010000117 DACCXW010000000
VERSION     DACCXW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.120"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(369..1238)
                     /locus_tag="IVY71_002337"
     CDS             complement(369..1238)
                     /locus_tag="IVY71_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7986368.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1258..2379)
                     /locus_tag="IVY71_002338"
     CDS             complement(1258..2379)
                     /locus_tag="IVY71_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7986369.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2381..2776)
                     /locus_tag="IVY71_002339"
     CDS             complement(2381..2776)
                     /locus_tag="IVY71_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7986370.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2795..3592)
                     /locus_tag="IVY71_002340"
     CDS             complement(2795..3592)
                     /locus_tag="IVY71_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7986371.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3594..4382)
                     /locus_tag="IVY71_002341"
     CDS             complement(3594..4382)
                     /locus_tag="IVY71_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7986372.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4396..4872)
                     /locus_tag="IVY71_002342"
     CDS             complement(4396..4872)
                     /locus_tag="IVY71_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7986373.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4944..7601)
                     /locus_tag="IVY71_002343"
     CDS             complement(4944..7601)
                     /locus_tag="IVY71_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7986374.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(7620..8024)
                     /locus_tag="IVY71_002344"
     CDS             complement(7620..8024)
                     /locus_tag="IVY71_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP7986375.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(8256..8513)
                     /locus_tag="IVY71_002345"
     CDS             complement(8256..8513)
                     /locus_tag="IVY71_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP7986376.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT         2706 a         1763 c         1220 g         2916 t
ORIGIN      
        1 tatttttact ctcacttaaa aaagcactaa aattataaga gagggaggta atggtctgaa
       61 caccaagaat tgttccaaca tcgaattgct taactaatga gttcaaagct taattatgtg
      121 gctcttatat ttaccgagcg tggattgcga tctctttagc gaaacaaaca tctttcataa
      181 aaaaaacagc catatccgct ttcttggata tgactattta ctactacttt actatctttc
      241 attcttcttg tctcacttat gataaggcgc tgggtatcat gagtgaatga tattgctgaa
      301 tacaaatagt ccgaccacat taagttgatc ggacttcata agcattttat tgactaatac
      361 ttttttgctt aagaattgat tctaacatct tttgctaaag ttaagcatcc tatagttcct
      421 gcattatctg ctaacttagg tgtgacaatg tattcttcga tatttggcgt atcgacatag
      481 ccgttaatta gttctaaaaa acgtctttgt actttctttt tcagatgttc ttgttgcatg
      541 acaccaccac caaaaattat aatatctggt gaaagcatca gggtcgtatt acaggcacat
      601 tgtgcaatat aatccgcctc gatttcccag aacggatctt ctatagaaag atcttgtccc
      661 ttggttcccg tacgcatctc gatagcgggt ccagatgcca acccttcgag acaatcatga
      721 tgaaaaggac aagtaccaga gaaagtatct cctgaatgtc tcttaactaa cgtatgcccc
      781 atttctggat ggctaaaccc ttcaacaaaa atcccatctt gtatagctcc tccaccaatt
      841 ccagttccaa cagtgtaata aacaacactg gataaacctt ttccatgacc tgaaacatac
      901 tccccgtaac aagcagcatt cacatcggtt gtccaggcaa ttggaatttc gaaatgctta
      961 ttcaacgttc caacaaaatc aaaatttttc caggcagttt ttggagtaga ggtgatatgt
     1021 ccataggtct ttgattcacg atggatatca atcggtccaa atgaccctac tccaatgctt
     1081 tctaattgtt ctttatagtg attaaagaaa tcaattacca aactcattgt ttcattcggt
     1141 gtagttgtag gaaaactcac tctttctaaa acctttaaat catcattgcc aatggcacaa
     1201 acaaatttag tcccacctgc ttctattgag ccatacattt cttactccac cgtcctttta
     1261 tctaggaatt cgttcttccc tgtctaatag tttttcgaaa aactcatgtg gttccttttg
     1321 gtaccaatag gcaacagaag aaatatcgtc ttgacgctca taaagcttga tgtcatcatt
     1381 tcctagggtt tggaaagtaa cacgtaaatt ttctttgaaa ctaatggggt cgggtaaatg
     1441 ccagcggtac aacccatgca tcggtaaagc atcatcgcca aaaccatgaa ctggatttgg
     1501 attaggtttt ccagcactaa aataatcccg ggtgtgatct cttttagatt gaaaaggata
     1561 gcccataaat agcgtattaa aattttgagc atatgccctt tctccaaagt tctttttatg
     1621 gaaagcccaa gcgccgccaa aataatcttc tgctcctgta gatgttattg tagggaaatc
     1681 ttcatctcca tctatataaa acttaaattc tccttccccc caccagtatc tttccaatgc
     1741 gcacaaagca aagtaggttc ctacataata cccagtgcct ttgattccat caataagggt
     1801 gtaatctttt ccttttgtcg tgatgggttc acggttccat ttagcatgaa aatacatcgc
     1861 atcatccggt acttcagtca atgcataatt aaatgtataa aaaatgtgct taataaactc
     1921 aggatgttca ttcgttaaag taatcttagc tttttttcga aatggcatct caaaatagca
     1981 attcatccct gatgttggat tcactacgat tggtaaggaa ttcacttcgc atcttgtccc
     2041 aaacccatta caaaaaaaat ctcctaatgg actttctaca gaaggagtgg attcatcatc
     2101 ccaataaata cgcaaaatta aatcacgtaa gacgaaactc ccagcgattg ttttgtctgt
     2161 cactgtcatc caaaaatgac gtaaaattcc agggccttcc atttctgcta aagtaattgt
     2221 ctctccagca tttatcggaa cagaaggtga cccttttctc ccaattccta gatgactcgc
     2281 tgtcattgcc gctttccctt tttcaccatt tttattttct ggtgaaatag ttctactttc
     2341 ctctcccttg tggagccata tattatttaa acaattcatt ttattcgtcc tctatccttt
     2401 cttcactttt ttgattattc atatagatga tttgttgttg tgcctccgca ataattgctc
     2461 caatatctaa attatcattt cctaactgca aagcgattga cattgccaag ggaagattca
     2521 ttcctgcaat tacgtgcact ctgtcagaaa tataggtaaa aaacttttga tttacacttc
     2581 ctccagccat gtcagttaaa acaattattt cgtctgtagt atccactaac tctttaattt
     2641 gttcatctat atcatcaatt tcttcgacat atgcacataa gctactaatt tgattatgca
     2701 tacctgttaa aaactctagt gtctctttaa aaccctcagc tagtattgaa tggctagcaa
     2761 tgatcaatct tctcataatt caaatccttt tcctttacgc taatattcca aatgcagcca
     2821 taaccatcgc aaaaacaata acgagtagaa tcaaaatggt catactgatt tttttcttac
     2881 tgagtagata atatgctgca gcagtaataa ttactggaat taatgttggt aaaattttgt
     2941 ccaacattgg ttggattttc atactaactt caccaaaagt gaatttaagt ggcgttgaaa
     3001 cactgataac tgaagcaatt aatgatccaa ctactgttag ccccaatact gaagcagctt
     3061 cagttaaata attaatcttt tcaccgtatt cagtaacaat cttcattccc tgcttataac
     3121 caaattcaaa gcctctcatt cggaaaaaga agattaccat gattaaaatg aaccataaaa
     3181 agattcctac aggattattt tctaaagcca tataagccgc aattgatccc atgatggttg
     3241 ggatcatcgt catcaaaaga gagtccccaa taccagcaat tggacccatc gtaccaactt
     3301 taaagtcatt aatcgcatct tttgcttcta atccttgctt ttcctccatt gcaacacaag
     3361 cacctaaaat aatggctgcc agccaaggct gtgtattgta atactgaaag tgattattta
     3421 aagcttcgat gtattcatca tcatctttgt ataattttct taaaataggt gacaatgcgt
     3481 aagtcacaga tgcaccttgc tgtgtttgat aattaaaggt acaaactgac atcaaccaac
     3541 gatagttcgc tttcctaaga tcctttttcg ttacttgata tttttcgctc attttattca
     3601 tcctccccaa taaacccaac atcagaagct cccgcagaag cagtgatttg tgcatctttt
     3661 tcagctctat gatacacatt aaatgctaaa gcgaatccta tgatcgccgc tccaataata
     3721 ggtaccttca aaaaagcggc taagaaaaat ccaacaagta aataactaat gtatcgacct
     3781 gtaggcataa agcgtaacaa catcgcaata ccaacagcag gcaacatttt tccggctaat
     3841 gttagcccat ccataaacca attaggtaaa acatccaaaa cgtaattaac agcagactgt
     3901 cctaaagtaa cggcaataaa tactggaatt gctgcagata aaccaaagaa aattggcccc
     3961 aaccacaaaa cactattcat tttcttaaat tctcgtttat tcgcatattc ctgtgatttg
     4021 cgaacaacaa aaccatttaa gattttgaca attacatcta gttgcacccc taacatacct
     4081 acaggtagtc ctattgctaa tccggcttct actccaagct ggctaccaaa tgccgttgca
     4141 ataattgcgc acgttccata gtccggtaca gatgagccac caaaaccggc aactcctaat
     4201 gacatcaatt gaactgtacc gccaactatc aaacctgttt ttatatcacc catcaaaatt
     4261 cctgtgaata atccccaaaa aactgcgtaa tttacaaaga tcatcggaat cccataataa
     4321 tcaacatgtt taataaatgc taatatagtc agcataataa tttgaaaaat agtaaaatgc
     4381 attatcctta ctcctttatc ctaattttga ttgaatttcc tgtgccttat ccgccggaac
     4441 aaattgtgta acaatttcta caccagaatc caacaatttt ttatacgtgc tgatattttc
     4501 atctgatgca taggctctag aagatagctg taatgcatta tctttaacaa cagtaccgcc
     4561 aacaattagt cggtcaattc taataccgtt atctactaaa tgaaggattg tttccggctc
     4621 tttcacgatg acaaatactt tttcctgatc gtatttcccc tttttaaaat tcgcaatcgc
     4681 tgtttcttca gtgataattg atacagccat tccagcaggt ctagctaaac gcatacttga
     4741 tttttggatg tcgttatttg cgacagaatc atcgatcacc atcactctgt ttgctttaga
     4801 cattggtgcc cattgcgtga caataatccc atgcaataaa cggttatcaa ctctcgacat
     4861 aacaatactc ataaatactt cctcctttat ctttcatttt cttacaagca attaccatgc
     4921 caaaacaaaa agaacttgat aaatcaagct ctttttacat aatcataaac gtatttaacc
     4981 tcatgaatcg ggatgtcgac actataataa tgttcaacga cactgaaaga ttgcttaaaa
     5041 tgtgtctgaa atttttgaaa ttcctcactc gtttcattga aataaatttc atcttccaaa
     5101 gtattttgtt ttactaatcg ttcaattaag caactaatgt gaacatataa tccgaaacac
     5161 gtattattat caaatgaaat tcctagatca ttttgtaaga tgtatatagc atctgaaacc
     5221 cgttctaata atttagtcgg atttaatatg gtcaattgct ccattaaatt cgttagacta
     5281 aaattcttca gaatattatt gttcaaaact ttcagttcat tatcattaaa gtgtctattc
     5341 aattgattgg agaatttact tatattcatt cctaaaatca tatcttcaat tgaaaagaat
     5401 tctacatctt ctagatgtgg atttagtgtt ccaacaataa aaagaatatt gttctcttta
     5461 cgaagctccg ataaatagtt ttcattcact aaagagtaat agtcacacgt ttctatgaca
     5521 agatcagtat cccttggaaa gctatcttca agtatccttt ttaatttaaa cgaagttcct
     5581 aatccgcttg cacaagaaca gacaatcata tccttcttaa tatttgatga ttcataaata
     5641 taatccacat tttgattttc ttttacttgt tcaagaatct gtttaagcgg taaatctaat
     5701 aatatctgat ttgcaacatc aatagccatt cttgttgaaa cattgtttag tagtccgaat
     5761 tttttttgag cgtctagttc caaatttaga taaattttct ccagtgaacc catatcaaca
     5821 agtaaaataa tttcttcata tccggtacac tcttgtaagt atctattaat tttgtctgca
     5881 atctcactac ttgaagtcat tagtggcata tcaatggcgt cgtatatata tctctcaacg
     5941 atcctattag cagtacttgc tatacaggtt gccgttagat ctccatgagc caaaatcagt
     6001 gctaaacgtt tttgctgaaa ttctttacta cacattaact taaataaaat aaccaaaaca
     6061 tttttttgaa tttttgttaa ataaatatcc cctatttgaa taaaacgatt gactaattga
     6121 acttctcgac tacaccaact attaatatca ttgatgaagg aatataattt atcgtccgat
     6181 gttaattgga tttgataaat ccaagaattc aaaaagatag acgttatttg aagataataa
     6241 tagtcaaatt ctatttgaaa atctctctct atttccttaa catactgtct aaatcgaatc
     6301 tcttgatagg tgggatatgt taaagctgaa ttattttgga tatcaacatt cttttgtcta
     6361 aaatatctat catatttctc aaaaagctgc tgcttatcag tgtctttctt ccacgtttga
     6421 taaagatact ctataagttc gttgatatta aaaacttcgt ctactttttc caaatcatcc
     6481 atttgaatat attttaattc attctgtctt ttcataacct tcatgccgtt gaaattcacc
     6541 cattgtggta aatgatacat ctgaatataa agttcttcca aatcatctaa gaaataaaag
     6601 gcattggcac aggtcaattg aatgatattt ttcaaattgc caacattttc actaaagcga
     6661 tagtttaata aaaaatctaa taattgttga gatatcagta ttttctttgc tagtctttct
     6721 tcttctgatt taaaaaatat cttgattaac tgttttcttt cataaagaga acgactgtct
     6781 aatgttggaa gtgttagctt tacaggtatt cttctcaaca aagttgataa tagtttatcc
     6841 tcaggtggtt ctgatgttgc aaatataatc cagcaacttg atgtgtacca gttttcatta
     6901 tcacccatcc gatgaaattt cctcgtatcc ataaactgaa aaagtttctc ctgacattca
     6961 ttgctcaatg catgaatttc atctaaaaat aagactccat tattagctaa ttctgctaaa
     7021 ccctttcttc catcttcggc tcctgtatat gcacctttta catgcccaaa aaaattggac
     7081 aagaaaagtt ctggattatt ggcatattca ctacaattaa ctgaaatgaa agatgccttc
     7141 tcttctatat aattattggc caacaaatcc ttatacacat aatttgctaa atagcttttt
     7201 ccagttccag tctcgccatg aagtaaaatg ggtaagccat ttggcggata gtagattgct
     7261 gctttaattt gagcaattac atttcgcaga cttccttgac ttccgataac cctttctatt
     7321 ccaaccctaa aagagctatc ataccatttt tccgcttcct ctgaagagct aaaaatttgt
     7381 tgatttggaa ctttctccgg taaagaaaag aagattactg gacgagtatt gatttttcca
     7441 aattctccat cttttacgaa ttcattcaaa tattgagaga caatattacg actaacattt
     7501 aatttttcag atattttctg tgctgtattg ttcccccatt tatttttaat ctcagagttc
     7561 tctatgacat aattgacaat gcgttctttt aattcttcca tcacagcacc tcctcgacac
     7621 tattcgaatt ttgccgacag tatttttcca taaatatgac ttgctaaaaa ggctttgttt
     7681 agatcattca attggaacgt tgcttgtttt gcttgatccg ctaagacgtt gaaaagatac
     7741 tctggcgaaa tcgcataccc caaaatatca acgattgttg caatcagatc ttcttttgat
     7801 tcttcatctg aaagtaattc acgatacaac atcgtttgtt tgcttactgt gtcatattcc
     7861 tctaaggatt catctgctaa aagaaccact tgttccaaca aacggtcgga taaatattta
     7921 tagaaaatca gccccaataa ataattttta tattctgacg catccatttt actacggaga
     7981 ttatctgccg cactaaataa cttctgattt aattctgcac ccaaaaagat acactcctat
     8041 ctcttattta aagttgctat ataccctctt attttagcat actcagtttt agaaaaagca
     8101 aaaaaataaa gaaacttatt cttgataaca agctacgtga cgtatatccc taacacaaaa
     8161 atagccatat cctttggttt ggatacggct atttgatatt actcattcaa ttatcaaatc
     8221 acagtaggtg tgaatctcat cgggatcggc aatatctact tctgattagg atcaaaggtg
     8281 attttctcaa taaaggtgtg atacaaagct ttaacttgct tcttctcact ctggataaga
     8341 aagcaatcca aactagtcaa tatctgttgg acatctttga cttgaatcgg ttccccttgt
     8401 tgttgaaaga tccctaagat ttcattctct cgaatttgta actgtcttcg tttttcagtc
     8461 aattcattta aacgatcttt taacatcgga aacaattcgg gactatcttc caaagcagct
     8521 tcctgttttt gctctaatag ttagattttg cgtaccattt tcttctttta ttttccttcg
     8581 attgctgatt ttaatatagt ctaaa
//
LOCUS       DACCXW010000118         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCXW010000118 DACCXW010000000
VERSION     DACCXW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.121"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..173)
                     /locus_tag="IVY71_002346"
     CDS             complement(<1..173)
                     /locus_tag="IVY71_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7986377.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVY71_002347"
     CDS             complement(593..1714)
                     /locus_tag="IVY71_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP7986378.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IVY71_002348"
     CDS             2042..3451
                     /locus_tag="IVY71_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP7986379.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVY71_002349"
     CDS             3448..4176
                     /locus_tag="IVY71_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7986380.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVY71_002350"
     CDS             complement(4304..5215)
                     /locus_tag="IVY71_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7986381.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVY71_002351"
     CDS             complement(5232..6239)
                     /locus_tag="IVY71_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7986382.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVY71_002352"
     CDS             complement(6236..8365)
                     /locus_tag="IVY71_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7986383.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IVY71_002353"
     CDS             complement(8370..8477)
                     /locus_tag="IVY71_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986384.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACCXW010000119         8379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCXW010000119 DACCXW010000000
VERSION     DACCXW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.122"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            624..1487
                     /locus_tag="IVY71_002354"
     CDS             624..1487
                     /locus_tag="IVY71_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7986385.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1616..3238)
                     /locus_tag="IVY71_002355"
     CDS             complement(1616..3238)
                     /locus_tag="IVY71_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7986386.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3487..4008
                     /locus_tag="IVY71_002356"
     CDS             3487..4008
                     /locus_tag="IVY71_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986387.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4194..5924)
                     /gene="spxB"
                     /locus_tag="IVY71_002357"
     CDS             complement(4194..5924)
                     /gene="spxB"
                     /locus_tag="IVY71_002357"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7986388.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6089..6907
                     /locus_tag="IVY71_002358"
     CDS             6089..6907
                     /locus_tag="IVY71_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7986389.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7010..7207)
                     /locus_tag="IVY71_002359"
     CDS             complement(7010..7207)
                     /locus_tag="IVY71_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986390.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7376..7954
                     /locus_tag="IVY71_002360"
     CDS             7376..7954
                     /locus_tag="IVY71_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7986391.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         2512 a         1517 c         1651 g         2699 t
ORIGIN      
        1 aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc atctacggca
       61 ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt
      121 gatgcagagc agctttttca caacctctcc actaaacggt gttctcctgc caactcctcg
      181 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga
      241 ctcggagcta aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct
      301 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt ttttctctaa
      361 tactcaggtc aaaacgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc
      421 tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
      481 atttcttgaa gctgaccact tctgtcacaa cctcttattt tgattagaac ttgcgaaaat
      541 aaaccggtta aagtgtgtct agcgcaagag atttgctata atagacgata agggtaaaat
      601 gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac agattcttgt tgtgatctgc
      661 cataccatgt attagcaaaa gaaaaagttg attttttatc aatgaatctc caattgaatg
      721 atcaagagtt agtagatgac ctcggaaaga cattcaatta tgaccgctac atgaatcagc
      781 tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt tggccgatat ttagaatttt
      841 tccgttctta tgcggaaaaa cagattcctg ttttatatgt ggctttttcc agtggattaa
      901 gcggctctta cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc
      961 ctgagatcta tgtttttgat acaaaagctg caagtattgg ggaagggctt ttagtattgg
     1021 aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa
     1081 gtaattattt gaaagtccat tcatgggtaa cagtagacga tctgaaatat ctagaacgag
     1141 gcggtaggat ttccaaagca agtgctactc ttggcagtct attgagtgta aagcctatta
     1201 ttacggtgga taaagaagga aggctgacga atgttgacaa agttcgagga agaaacaagt
     1261 cgatccagac aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg
     1321 tttatgtggc ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg cttttggaag
     1381 agcagataaa gatgaaagat gtgaagcttt atcccttagg accaacgatc gccagtcaca
     1441 caggctatgg ctgtatcgcg attttttcga tgggaatcag tcgataaaag cagtaactca
     1501 ttctgtaaaa aagcccagaa attgattgta acaaaaacag cgactcttaa aagatagatg
     1561 agcatcctat ctcttagagg tcgctgtttt tttatttttt aagctaaata ggccattatg
     1621 cccataactc gttcaattgt ttttgaatat ctgcattttc caaaaattca tcatatgtcg
     1681 tatcagctcg atcgatcaca cctttatccg aaacggcaat gatgcggttt gccaatgttt
     1741 ggataaattg atggtcatgt gacgcaaata agattgagcc agtaaatgcc atcaagccgt
     1801 cattcaatgc tgtgattgat tctaagtcta aatggttcgt tggatcatct aatacaagta
     1861 cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg gactttctct ccaccagata
     1921 aaacattcac tggtttcaat acttcttcac cagaaaacaa cattcttcct aagaagcttc
     1981 ttaagaaggt gttatcatct tcttctttac ttgcatattg acgcaaccag tctaaaatcg
     2041 ttagatcagt atcgaattct ttcgttgtat cctttggtaa ataagaatta cttgttgtca
     2101 caccccaacg tactgttcca gtatctggcg taatttcacc catgataact ttgaatagcg
     2161 ttgttgttgt tatatcattt tctgcgacga aagcgacctt gtcttctttg ctcaaattaa
     2221 aagatatatc atctaaaatt ttctttccat caatcgttac acttacattt tctacctgca
     2281 ataagtcgtt tccaatctca cgttcaggtt tgaatccaac gaatggataa cgtctagaag
     2341 aaggttggat atcgtctaaa gtaatttttt caagcatttt tttacgtgaa gttgcttgtt
     2401 ttgattttga tgcattcgca ctaaagcggg caataaattc ttggagttct ttgatttgtt
     2461 cttctttttt ggcatttgcg tttgcttgaa gtttagccgc tagttgacta gattccaacc
     2521 aaaaatcata atttcctaca taaagtttga ttttaccaaa atctagatcc gccatatgtg
     2581 tacatacttt gttcaaaaag tgacggtcat gggatacaac gatcacagta ttttcaaaat
     2641 tgatcaaaaa ttcttccagc caattgatcg attggatatc cagaccgttt gttggttcgt
     2701 ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc taataacact tttacctttt
     2761 gtccaccagt taattcactc atttgctgat ggtgcaattc ttcagggatg ttcagacctt
     2821 gcagtaaaac agctgcttct ggttcagctt cccatccatt aagctcagca aattctcctt
     2881 ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat
     2941 ctttttcttt cagcacttca tacagacgct tgtgtcccat gatgactgtt tcaagtacag
     3001 tatagtcttc atagtcgtaa tggttctgtt tcaatacggc catccgttca tctggtccca
     3061 tagtaacgga acctgtggaa ggctgcagat cccctgataa cactttaagg aacgttgatt
     3121 tacctgcacc atttgcacca atcaagccat agcagtttcc gggggtaaat ttaatattca
     3181 catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac atcatttaca gtaatcaatt
     3241 tttttcctca cttttatcat tattagtttt gacaagtctt atagaattat ataggggaaa
     3301 ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa
     3361 ataaaaataa aagcggagcg aaaatagcta ctataggatt tattagtttg caacagcata
     3421 atttttgacg ttatttataa gagggagtac tataaaactg taaaagaaat gaaagaagga
     3481 ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat tgattttggg aattttattt
     3541 gtacttgtct cattgttatc atttcaagac cctgtcggaa atttagttgc aattgtagta
     3601 gtctttgcga tttttgcctt tattaaaggg atatttgaac tgtttgtacg gaacagatta
     3661 aaagagctaa caggatataa aggaaagatg cccttaataa tcgggatcat cgatattcta
     3721 gttggcgtct ttttcttatt caatattggt gcaggagtcg cagcattgcc ttttgttttt
     3781 gcagtatggt ttattgctga ttcagtttta gcgttattta ctgctgactt ggcaagagga
     3841 gtaagtgaag gatattattg gtttacgatc atcgtcaata ttttaggaat tttattagga
     3901 attttcttat tgtttaatcc aatttcttct gcgttgacat taagcttttt agttggattc
     3961 tattttatgt tatttggtat cacacatatc gtctatgcat ttagataagt aaaatcaaac
     4021 aaataaaaaa gccggcttcc gattacagtt ttactgttgt aattggaagc cggctttttt
     4081 atcatggaca aatatgcacg aggtgatttt taggtgtcga gacaaattta tgcaaaaatg
     4141 tgagaagcta tatacacaaa ttttatctta atactcgaag catagcgcag ttgtcacaac
     4201 ctcttttcat ataattcatt caatactggc aaatcatgga cttcatattt ttctttgaat
     4261 gcttggattt cttcttcaga atattttctt ggatctaaat gcagatcttc tactggcaat
     4321 gggcgcttgt tattgatctt cacatcgatt actacaggac gatctgattt tgcagctgca
     4381 tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa agctatttgc cccgagcgct
     4441 tctccaactt taccgaaatc tgcatcttct aaaaatacac caaatttctt ttgttccgta
     4501 tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac attaataatt
     4561 ggaagctgat atttgacttg tgtaatgata tcttgcatga ccatagcaaa cccgccatca
     4621 ccacttaagg taaatacttg tttttctggg tagcttagtt gtgcggcaat tcctcctggc
     4681 acaccattac ccatagttgc gaaccatcca gatgtcgtat gtttttgttt accgttcata
     4741 ttcaataaac gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg
     4801 tcttcagcaa tacgattgat ttctttatag acagcctctg gacgaatcgg ttcttcctct
     4861 cgatcttcaa aagatttcaa ccaatttacc catttttctt tattttcttg atttgctagg
     4921 taccagctat cagcagggcg agcatcccct aactcgacaa gtctttgcaa agctgtgttt
     4981 gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc caaattttga cgcatcgata
     5041 tcgatttgga taaattcagc agttggatta aaaaatgcac gtccaaatgg gaagtcgcta
     5101 ccgacaaata agatcagatc ggcttctgcc aatgcttcat ttccgggttt agtagcgaca
     5161 cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa taattccttt tgctagaacg
     5221 gaagcgacaa caggaactga aaaatgttct gagaattctt tgatttgcgg aaatccgttt
     5281 cgcgttcctt gaccaacata aagaactggt ttttttgctt tttcaagata aggcaaagca
     5341 gctagcaaat ctttttcttc tggtaatatc acacttgttt tgtgagtgtg agcggtggag
     5401 aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag ggatagtcac gatagccacg
     5461 cctttatgct cataggcggc tttaatggct tcatctacta catgcggaag actttgtggt
     5521 gtcatgactg tacgattgta tacgctaaca tctgcgaaaa taggattttc atttagttcc
     5581 tggaaagagt tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc
     5641 acatggtcca tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca
     5701 gagccgaaga cagcgccgac tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg
     5761 ccgacttctt catgacgaac ttgaatgtag ttgatttcgg ttttttcttg atatagcgca
     5821 tccataattg aattgaaaga tcctccggga atgccgtaaa tatggtcgat accccagctt
     5881 tcaagaactt ttaccattgc gattcctgca ttgatttttg acatggtgtt tcactccttt
     5941 agcaatgatg atacataaat tttttgtatc tagagtatag aactttttat cgaaaatccc
     6001 aactaattcg tcttttcaca aaaatgtagt catgtttttg ggaatatgct agagtagtac
     6061 atagatagat tcaagagagg ggaatggcat ggttgaatgg ctattgagac ttgaaccttg
     6121 gcagcaagca cttgctggga cagcttttac ttattttatg acagctcttg gtgccgggtt
     6181 ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc
     6241 tggtgtcatg attgctgcaa gtttttggtc tttgttagat ccggctatta caaaagcaga
     6301 agaaaatgga gatattgctt ggctagttgt aagtattggg tttggtttag gcggtgtgtt
     6361 tttatatatg gcagataaaa cactgcccca tatgcatttt ggaccacaac atgaagcaga
     6421 aggattgcct acccatttaa aacggacgat cttgcttgtt ttttctatta cattgcataa
     6481 cattcctgaa ggtttagccg tgggggtagc attcggtgca gcagcaactg cggataatcc
     6541 gacagccgct atattagccg ctgtttcggt ggcgctaggt attggtatcc aaaacttccc
     6601 agaaggagca gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt
     6661 tgtttacgga caagcttctg gaatcgttga accaattgct ggagtgattg gagcgctact
     6721 agtaacgaaa gtggagctgc tgcttcctta tgcacttgct tttgccgcag gagcaatgat
     6781 ttatgtcgtg gtggaagaat tgatccctga agcacagcag acattgtcca gcaaacgtca
     6841 ttttgcagtt tttggcgtca tgagtggatt tatcattatg atgattttgg atgtggcatt
     6901 aggataaata aaaaaaatat atatgatata tcaaataggg ccgcgaactt atatcgcgac
     6961 cctatttttt tagtaagtta tatcataatg aagtgaatgg tatcatacat taataatagt
     7021 tttttctatg tgtatttcct ttgatccagc gaacaagcca gatgataagt gcgattggga
     7081 ttagaacttt gaccgcaaac cataaaagcc cgcttaggac actgacaaaa agactgatga
     7141 cgaagctagc tgcaattagc actaaaagaa ccatgaagat atttaagaac atattttttt
     7201 gacgcataca aaaccctcct cttgttcatt ctttactata gaaaaagtat aacagggatt
     7261 gtttaaaaac acctccaact gaagactgat ttttacttca tctacttaaa tgatcaaaca
     7321 attgcttttt ctctggttta tcatctaaaa tagagcggaa aaggaggaga atatcatgcc
     7381 taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg attatcatga ctattttagt
     7441 tttagaactt catgaaccta acggagacac gtgggaggcg ctcgctgcat taggttaccg
     7501 attttttatc tatattgtca gttttatcat tttagcgatt tattggaaca atcatcatca
     7561 tttatttcaa gttgtacata agataaatgg acgggtctta tgggcgaaca gtttctttgt
     7621 ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc agtgagtaca tcaatagcct
     7681 ggctccagaa atgacttatg gttttgttac cttgaccgct aatattgcgt attatatctt
     7741 ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa gcattgaagg cgatgttgaa
     7801 taattacaag aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg tcttagggta
     7861 tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg attctagctg cttggatcat
     7921 cccaagtcgt accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag
     7981 aaaaatatag aaaaaataaa taaacgatca aatatccgag tgatactagt attcaaagag
     8041 ggatgcagat ggatcaaaaa agaattcatg taggactgat tactagtttt gtcacgatgc
     8101 ttatttattt ggatcaaggt catttgactt ggacagtagt tggtgtcgta ggatttttga
     8161 cattattcac agcaataaaa aaataaacat ctttatgtaa tctgatgttc tatctattta
     8221 agaaaagcat gtttacagca gtatgattct gttgtaaacg tgtttttatt tgtggaaaat
     8281 ttaattttta gaaaagtgta tcgaaaaata gatagttagt ttttccacag tggataactt
     8341 cgtaagagat aaaaataaaa aattagtcaa agaagggcg
//
LOCUS       DACCXW010000120         8093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCXW010000120 DACCXW010000000
VERSION     DACCXW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.124"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..243)
                     /locus_tag="IVY71_002361"
     CDS             complement(<1..243)
                     /locus_tag="IVY71_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986392.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELT"
     gene            complement(451..1377)
                     /gene="glsA"
                     /locus_tag="IVY71_002362"
     CDS             complement(451..1377)
                     /gene="glsA"
                     /locus_tag="IVY71_002362"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7986393.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1400..2611)
                     /locus_tag="IVY71_002363"
                     /pseudo
     CDS             complement(1400..2611)
                     /locus_tag="IVY71_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2646..4259)
                     /locus_tag="IVY71_002364"
     CDS             complement(2646..4259)
                     /locus_tag="IVY71_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP7986394.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(4371..4580)
                     /locus_tag="IVY71_002365"
     CDS             complement(4371..4580)
                     /locus_tag="IVY71_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP7986395.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            4671..4859
                     /locus_tag="IVY71_002366"
     CDS             4671..4859
                     /locus_tag="IVY71_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986396.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            complement(5569..6783)
                     /locus_tag="IVY71_002367"
     CDS             complement(5569..6783)
                     /locus_tag="IVY71_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7986397.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(6911..7381)
                     /locus_tag="IVY71_002368"
     CDS             complement(6911..7381)
                     /locus_tag="IVY71_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7986398.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(7378..7620)
                     /locus_tag="IVY71_002369"
     CDS             complement(7378..7620)
                     /locus_tag="IVY71_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP7986399.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
BASE COUNT         2300 a         1724 c         1300 g         2769 t
ORIGIN      
        1 tgtgagctca gaaagagcaa taccatgttt gaatacgagt ttatacttat gcttctctct
       61 tacttgttct tttatacaat attctagaca ctcggctaga ctttctagat cgtgaaggat
      121 cgttggtgta gtcaccctta gagaaatagc tgtttgttcg atgtctattt cttggaattc
      181 aaataattgt tcacacagat acacttttct aaatatattt cgctcgatgt aattttccag
      241 cattcctgat acacgcctcc agtttttctt atgattttat ttaatcatat ctgtaaatcg
      301 tatttttgtt tcctttttat ttagaaaagc cctttcaaaa accaataaaa aaagaaactg
      361 ggacaaaagt caaacaagct cacaatttca gcctcattgt aggtagcttt gagccgtttg
      421 gacttttacc ccagcctctt tcaagaatgg ttattcaaag atgtcagcgt ctagttcttt
      481 gactacatct ttcaacaatt ggataccagc tgcgctgtta ccaaatggat ctagtgccgg
      541 gctgaagaca cctatgccgt aacgatttgg cacagctgcc atcaacccgc cgcctacacc
      601 gctttttgca ggtacaccaa catggacgga aaactctcct gattcatcat acaaaccagc
      661 agttgtcata attgatttga cgattgtagc actttcttca gtaatgattt gtttgccatt
      721 ccaaggagca attcctttat ttgccagcac agcagctaat tttgctatgc ctgtagcggt
      781 gacattgagg gagcattgtc tgaaataagc atcgagtatc tccggtacgt ctccttcgat
      841 catttgatta cctttcatat agtaagcaag agagcgattg atatcacctg ttcttgattc
      901 agaatgatag atttcttcat tcaatgtgat attaggatca tcacagatct ctttcataaa
      961 tgacaagatt cgctcaaatt tttcatctgc atctttaccg gcaatcaacg aggttgttgc
     1021 aatcgctcct gcattaacaa aaggattcat tggatgtttg cgatgattga tttccatatt
     1081 catgatagaa ttgaaggcaa aaccggttgg ttccgtgttg attgtttgaa agactttttc
     1141 tattccgttg tctttgattg ctaataacaa tacgggaact tttgacatgc tttcgatggc
     1201 aaagcgcaca tcggcgtctc ctccctcaat cagttgtttt gtatccaaat cataaatcgc
     1261 catacctaat tgtttgggat ccacttcagc taatgctgga atgtaatccg ccacttgtcc
     1321 tgtttgataa tagtggaaat tttttccaat caattgttct aattctttta cttccatttg
     1381 aataaccagc tttcattttt tatttttctg catgctggaa tttatgacgg catgcgtaga
     1441 tcgtaaatgg aataactaag acgataagat aagaaacaat cagaatcgtt tcataaatcc
     1501 catgctgtcc agctgcgata ctagcaggtg gaacaaatga gatacaaaaa gcaaaaagtg
     1561 aagtcaaaag acctaaacaa gcaacgatta cttttacaac ttttccgcct ggaacctgat
     1621 aggctctttc aagactgtca tctttaaaaa ccagcttcaa atagcccgcg aaaaacataa
     1681 agtacaacat caggtagatt acaacagtca atgacatcgc tgcaagaaat gaaagattat
     1741 tccccccgcc gcttaatgta aagacggctg cccagattgt tgcaacgatc ccatttgcga
     1801 tcatcaaatg agcaggaaca ccgtgtgtgt tttcttttgc aaaatattca ggaagtaatc
     1861 cttcttctgc tgctacgtga agtgcttttg aaggagaggt tacccaagaa gcaatttctc
     1921 cgatgacacc taaacaaaca agaatcgcaa gaattttcgc aatccatgca agatttggat
     1981 caatgtgtct taataggaat tctaacgatt gaatcacccc tgtattaaga gacaaaccat
     2041 gttgaggtac tgtagtagcg acggtcaatc cgccaaaagt atctaaaatg attgccagaa
     2101 taaccaatac aaacattgct aatggataat cctttttagg gttttccatt tcatttgcat
     2161 gactggcaga tgtttctacg cccatatatg ctaaaataaa ggaaacaaaa acaaccagcg
     2221 taggcaattt cgaaaaatca ggaatcaatg ttttcataga cagtatcgtt tgtagtggag
     2281 cgcctgaagc gacataatgg atccctaagc caaacaagac taatgctggc aacaagatac
     2341 cagctacaaa accgatccga gcaatcaatg aagtgtattt ggttcctttt aactgtgaga
     2401 gtaaaatagc ccagaaaata atcaatagga ttgccaattt cagatattta ttgttttgta
     2461 tagcaccaat acccgttgca taggataatg caccagttaa gaaatacagc atggtcaagt
     2521 agccaactgt tatctcaaac cattggaaaa agattgcggc aaaaccgaat cgttttccca
     2581 atgtgcgact gacccaagta tagacaccgc cggtttccct tagttaaaaa tattactctt
     2641 gttgattact caactcttcc aataaatcgg taatatatct caatttcctg tattctttcg
     2701 ttatcctcat tcgttgttgc ttcatgtatg aggatttttt ctatcatggc atttaataat
     2761 ggtgctgtca gttcctttgg tacggaatat tccttgatta actcaatcca cttttcagca
     2821 cctatcatat cctgttccat tttacttagt tcttctctta ggcttgttat ctgctgttcc
     2881 agttctatct gttctttttg gtacttgctg gacaacatca caaaatttcg ctctgttatc
     2941 ttctcacagg ctctatcttc atacattttc gcaaataaac ggtcaatctc attttgtctg
     3001 ttctcggctt tcttcaatgt acttgccttt ttcttctttt cccgtattct ctctgcattg
     3061 ccagctttct gtatcttgtt caataccttt tcttcgtcct gctgtactgc cttagcccaa
     3121 tactgcaagc gttccaatac ggcttgatac agcacatcat aacgaatgta gtgcatagaa
     3181 caattacctt tgccaaactg cccgtagtaa ctgcaagcat aataactgta aggcgtttta
     3241 tttgtcttat tcgttccaaa tctcatagac caaccacaat ccgcacactt gacaagccct
     3301 gcaaaaatcg gcgttgcctt ttcctttgtc tgtctgcgtc ttgattttat ctgctcctgc
     3361 acacgataga acacttcctt gtcaataatc ggctcgtgag tgttttctac tcgaaaccat
     3421 tcactttcgg gcttacgcac tttctttttg ctcttgaacg ataccgtaga ctgcatattg
     3481 tgaacactgt ttccgatata aacctcactt ttcaatatcg ccttgacatg agcaatcgtc
     3541 cactcataac gcttactttc gggttttccc tcaaagatat gtgcaaaagt accgtatctt
     3601 gtaaaattta gccatgacgc tgtcggaact ttttctgctc ttaactgctt tgtgattttt
     3661 gcacttccgt aaccttgata ggctagggaa aagatttttt caataatcca ttttgtttca
     3721 tcatcaacca acagttttcc tttgatgtca gggtgtttct tatatcctaa cggagcataa
     3781 gcaccgtaat gagccccctc cgcaaacttt gtcttaaatg ctgatttgac tttacggctt
     3841 gtatctcttg ccacccattc attgatgatg ttcttaaatg gtgcaatctc actttcgcct
     3901 ttttcgctgt ctacaccgtc atcaatcgct atgtagcgga cattatgact agggaaatac
     3961 aattctgtat attgtcctgt cataatataa tttctgccaa gacgggaaag gtctttcgtt
     4021 acaacacagt tgatttttcc tgcctctata tcctcaatca ttctttgaaa actcggtctg
     4081 tcaaaatttg ttcccgacca gccgtcatca atatattcat caatgacatt caaatgatgt
     4141 tcttttgcat ataaacgcaa catacttctc tgtgtggtaa tgctggaact ttcgccctgt
     4201 aattcatcat ctcggcttaa cctaagataa agtgcagtat tgtaaatctg ttgtttcatt
     4261 gtcagttatc ctcctttata actaacccgt gtttacaata cctgcttgca agtaaagtat
     4321 aacacgggta ctttttgtca ttcaatctat ttcttacaac tgcaccgatt ttatgcgatt
     4381 gtctgctttg ctttttccag catgacatca gttagaagtt cttccattgt tttgcccttt
     4441 tcggaaaaat gctcttttac tacaatcttt gtctttcctc tgttgccaat gccacacttg
     4501 ccatttttct tttgaattgt ttttacagca ttatcaataa cacatacccc ctttctgtaa
     4561 aatctttcta agcaattcaa cggcttttgt catagcccac aggagttcca cctctgcata
     4621 gttcttatgt agccgtaccc attgcgtgcg acgctcttaa tgctcaagct gtggctgtac
     4681 gggagtatca ttatctgaca gtacaggtca tggcgataaa agaggacaaa tcttttacaa
     4741 gaacacaaat aggttttcgc tcgctcttgc aggggaaagg tcgtggcgta tttgtttcaa
     4801 cggctagctg tctgtcaaac gtattgaata gctttattca gttgtcaaag aacgattgaa
     4861 agaaaaaaat cgtctttcct atatatcgcg ttggaaaaga ggagaaacgt aaccaaaatc
     4921 caaaactttt tccattatta gtacaaatgg aacggttcat tttggcaatc aacggttgat
     4981 tttctccttt ttcactacta tctgtacagc aaacgtccat ttgctaagtt taaatggaaa
     5041 aattttaact tcttttcatg caccatatag ggatagaaaa tgtcattttg ctaagttgat
     5101 gatgaaaaaa gaatattttc cttttcacac cctaactacc caaaccatac cgttactgcc
     5161 aacaatagaa tgaaatattt tctttgcacc atatagggaa cggaaacctt aaattgttaa
     5221 gttgagaaca aagtattttc atcttttcac atcaacactc aactgctaat gcctgtttga
     5281 atacaaaatg atgatgtttt tcctcttttc acagaagact gtccactaat cacgaatttc
     5341 cgtcattttt tcaaaacttt tttcttcttt cacttcatat agggacagca agccccgatt
     5401 taatgaccta ttttcaaaaa aagtttagat ttccttttct cgcaccatat ctgtacgcaa
     5461 gtagccattt tgttgcagat ttaataaaaa atttattttt cttctttcac ttcacatagg
     5521 gaatgaaaac agctctttgc tgactaaatc tgaaattttt caattacatt aaaaaatacc
     5581 gtcattctct tgtggaacag cggtatctat acgttcttct atggttgatt tctctaattg
     5641 taataaatgt ttatgcttgt cttttagttc tgcctgctca atcatatctt tcagtatcgt
     5701 tgtacgattt tctctgtcta atgcctgtac catttttatg gttggtgcga cttgtctttg
     5761 tagccaatgc aacgcttttt gtaaagtgta aggctctggc tttgtggtta agcgtatcgg
     5821 ctctctgttt tcccctacaa accatgccca atcatcattt gttatccatt gactttttgg
     5881 cttgtcgtcc tctctgtcaa caaagcggac ataatgattg atgatagaaa aagcggtgcg
     5941 ttcagcgtcc cgataggtca gtaaatctac aaccgcataa taggctcgtt cgttcttcat
     6001 gcgtatttca aaacggttct taatctccgt atcttcaatt tctgtgccat tcttgacata
     6061 ctgctcataa tttttctgat aagcacacat atataattcg ctacttgttg aaccgagata
     6121 taaggtttct cctgtattct ttggtaaatc gctaccgcat ttttctgtac cgctgtaatc
     6181 tttctgcata cggaagtagg acatacattc gccagccttg tatttttcct ttaactttgg
     6241 aatatccaat attcctgctt tatcattgat agccaaatca agccgtttca tcacaccacc
     6301 agccgtcata caatccagca tgaagtcata ccatgagcgt tcctgtgcca gcagataaca
     6361 ttccatttgc cgacagcctt tgcctttcag ttccaataaa acacctaaat gctcctgcat
     6421 ggaacacatg atattgatgt cgccaagtac atatttgctt tcatatccgt attttccata
     6481 atcttcataa agcatgtaat tagatttcag ttgtaataca tcacggataa tcgccaatgc
     6541 gtccgtagta ggaaagcgga cacgaaagta atcaatcaat aagaatagtg gttcttgcgg
     6601 attgaaacgc tcaatctgtt tttctatgat ttctcttaat tcgtcattca atggctgttt
     6661 tcctttttca atgcggttgt aatattcacg gctgatacca caggcaacag caagccttgt
     6721 ttgtgttact ccgtattcct cacgcttttg tcttagttct tcaataaagt ttttatcatt
     6781 cattcttcaa ttcctccaat caaaaaaggc aactaccata attgataatt gcctgtcatt
     6841 aaaaatctgt atagaaaaag cagtcaatcc taagactaac tgctttgtgt gtatggatat
     6901 aaaattgctt ctaatcttga ataggtatcc aaatttcaat aactgaatta tcactattcc
     6961 aatgaaaacg ataatcgtat ttttcaaagt gtaggataat atcttttata ggaatatacg
     7021 atgtactagg aataatttct tcataaatct tgccataagt ttcatatatt ttatccatag
     7081 caccaatatg ctccacaacc aaatatttat aagacggtat ttctttatac agaaagccct
     7141 ctggggtaat ggttctttgg ggaatagaac agaaataata aagttttcca tttcttcttt
     7201 ccataaaagc atattttacc caatttcctg attgtgaaag atacgctttt ttcaaactac
     7261 tgttaaactt tctccaaaac tgtgtactaa tctgaatatt ctttttttga taattagtca
     7321 gttctacttc ctgcccaata actgcaaatg cgtttaattc ctttatagaa aaatttatca
     7381 ttttaccttg tgagcagtga taacggtata actgtacgca tttaccgtta taatacaatt
     7441 atcaatcgtt atataccagt tttttccttt tcttgtgatt tccgcattag aaaggctaat
     7501 tttttctttg caccattcaa ttacattttc tacattcaaa gataagttct ttttaattct
     7561 aataacgcca agttcagttg tgtgaagtct tcctaagttt tccagtaatt cattttccat
     7621 attttgatac ctcatttttc agcaaattta tttgtatgtt ttatacccca attataacat
     7681 tctcatatca gcactacaat aaaaataatt aagcaccaat aatcttattg aacatctcca
     7741 accgtaaatc tttcacacca tcagtgataa acactgagct gacagcaatt acgacaacta
     7801 ccataattgc ctgcacttaa atttctgcaa aagaaaacga caatcaattt cttatttttt
     7861 gaaatcaact gtctttctca tatttactat tcaattttca tagtgatata ggtacaattt
     7921 atatcactac tgtaaaaatg gggttttgcc ttataaatac agcgttttcc gcttgttagt
     7981 gtatagtgtt tcttgttttt tgtgcccccc ttgttagata acgggggctt tgaacggctc
     8041 gcaagctcgc cgaaccgccg aagcgaaccg cccccttgca agcatggcgg aaa
//
LOCUS       DACCXW010000121         7949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCXW010000121 DACCXW010000000
VERSION     DACCXW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.126"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            216..1982
                     /locus_tag="IVY71_002370"
     CDS             216..1982
                     /locus_tag="IVY71_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7986400.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2084..3343
                     /locus_tag="IVY71_002371"
     CDS             2084..3343
                     /locus_tag="IVY71_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7986401.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3490..4791
                     /locus_tag="IVY71_002372"
     CDS             3490..4791
                     /locus_tag="IVY71_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7986402.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4794..5645
                     /locus_tag="IVY71_002373"
     CDS             4794..5645
                     /locus_tag="IVY71_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7986403.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5658..6473
                     /locus_tag="IVY71_002374"
     CDS             5658..6473
                     /locus_tag="IVY71_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7986404.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6624..7790
                     /locus_tag="IVY71_002375"
     CDS             6624..7790
                     /locus_tag="IVY71_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7986405.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2584 a         1369 c         1591 g         2405 t
ORIGIN      
        1 ggagaaattg atcgcttaag ataaattcag gacactaaat agtacaggaa gtagattaaa
       61 acttttaaaa gttttaagtc tatctatttg gcaagtttaa aaaataaaaa ttcggtatca
      121 aaaaactgtt accgataaca tttttgaaag cgttgtcttt tgtttctcta tcacttatag
      181 tgatgacgtc atattaaaac acaaggagga aatcaatgga tacagctgca atttatcatc
      241 gaccggaaag tgaatatgca tttctatata cgaatacaag atttcgtatt cggcttcgta
      301 ctaggaaaaa tgatatcaaa aaagtatatg tgctttgtgg agatccatat accataacaa
      361 ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg tctaagtacc aatgttcatg
      421 attattggga aattgaagtg acgagcgaaa caaggcgctt acagtatgcg ttccatgttg
      481 ttggggaaga taacacggac tgtttttacg gtgatcaagg aatttttcct tatcgagaaa
      541 atgtgattac tgaaccaaat ttttattttc gaatacctta ttttcatcaa atcgatcgat
      601 tcactattcc agaatgggta aaagaaacag tatggtatca aatttttccc gaaagatttg
      661 caaacgggga caaagcaaat gaccccgaac acactttacc ttggggaagt aaagatccag
      721 accgagaaga cttcttcggg ggagatttgc aaggtgtgag ggatcatttg gattacttga
      781 cggaccttgg cgtcaatggc atctattttt gtcccatatt taaagccaca tccaatcata
      841 aatacgatac gattgattat tatgaggtgg atcccgcttt tggcgataaa aaacttttaa
      901 aaaacttgat tgatgaagca cataaaagag ggatacgtat catgttcgat gctgtattca
      961 atcatatggg tgtccattca ccacagtggc aagacgttct caaaaacgga gaaaaatcga
     1021 tctataaaga ctggttccac atccgttttt tcccagtaga ttcttatcag atgactgact
     1081 taccagaaac tgctgaaaat atcccatatg atacttttgc ttttactcca ttcatgccga
     1141 aactaaatac agcaaatcca gaagtgcaaa aatatttgct ggatatcgca acttattgga
     1201 taaaagaatt cgatattgat ggatggcgtc ttgatgttgc taatgaagtc gatcatcatt
     1261 tttggaaaaa attcagagaa gcagttacag aaatcaaacc agacatctat atccttggag
     1321 agatttggca ttcctctcaa gcttggctgc aaggggatga attccatgca gtgatgaatt
     1381 atgcattcac agattcgatt aaagattatt ttgcaaaaaa gaaaatcaca gcttcacaaa
     1441 tggtcagtgg aatgaatcat cagcaaatgc tttacagaga tcaggtgaac gaaggaacat
     1501 tcaacttatt agattctcat gatactgcca ggatcttgac cctttgccaa ggtaacaaag
     1561 aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa agggtctcct tgtatctatt
     1621 acgggacaga aatagggatg actggtgaag atgacccgga ttgccgcaaa tgtatgatat
     1681 gggagaagga ggaacaagat ctagagctat tcggttttat aaaagaactt gtttctctaa
     1741 ggaaacagtt gtctaagatt atttctgaag gttctactca gtggctaata gtatatgatc
     1801 gtgaagataa gctctatttc acacgggaac tagagggaca gatcatctat gtttacttta
     1861 atcagtcaaa agaaccttgg gtagtagaac aggaaaatga ggtgattctt agtcaaaatt
     1921 gtcagttgtt agaagacggg aaagcagaga tacgacaata cggatttttg atcatgtgtt
     1981 gaaaactgtt accggtaaca acaagaaagc gtttgcaaat gaagatttgt gcccattata
     2041 ataagggtgt aacagcacgt tgaatacaca ggaggaaatg aagatgaaaa aaagaagtgt
     2101 gaaacagttg ggtttagggt taatctcttt aggaatctta ggcggattag cggcttgtgg
     2161 caatggtaac tcttcagcag aaggggaagc cgatgaagga aaaacgttaa cagtttccgt
     2221 tgattctgga tataaagatt atataaacga aataaaaaac actttcgaaa aagaaaacga
     2281 tgtgaaaatc aagctagtag aaaaggacat gttcgatcag ttggaatcat tagcgttaga
     2341 tgggcctgct ggaaagggac ctgacgttat gatggcagca tatgaccgga tcggtgcgtt
     2401 aggacagcaa ggacatctgg cggaagtaaa actgggaaat gaagcagctt acgatgaaac
     2461 agacaaagcc caagtgactt atgatggaaa aatctacgga gaacctgcag taatcgagac
     2521 ccttgttttg tactataaca aagatttagt cgatacagca ccatcaacgt ttaaagattt
     2581 ggagaatcta tccaaagaca gtcgttttgc ttttgagtca gaagcaggaa aaaatacagg
     2641 atttttagct aaatggactg acttttatta ttcctatggt ttaattgcag gttacggtgg
     2701 ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc ggtttgaata atgcaggtgc
     2761 tgtagaagga atctcttatg caacagattg gttcaaaaat gtttggccaa agggaatgca
     2821 agacataaaa agtgcaggag acttcgccag ccagcaattc atgtccaata aaactgcagc
     2881 aatcattgac ggtccatggc aagcgcaaac atacaaagaa aacaatatca attatggtgt
     2941 agccaaaatt cctactctaa ataatggaca gccttatcaa ccgtttggcg gaggaaaagg
     3001 atgggtagtc agcaattatg ctaaaaacaa agacctctct caaaaatggc ttgattatgt
     3061 aacgaatcag gaaaatcaag aaaaattctt tgaaatggta aatgaaatcc ctgcaaacca
     3121 acaagctagg gaaacagcaa aggctaaaaa cgatgaatta acaacagctg tgattgaaca
     3181 atatgaaaca gctcaagcga tgccaaacat cccagaaatg ggcgaagttt ggacaggtgc
     3241 agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca ccaaaagaat ccgcagatga
     3301 agcagtaaaa acaatcagtg aagccataga acaaaaatac tgattaaaat gataaccgtc
     3361 catttttata aaaaatggaa caggtgcttc agataaaagt ggcaagagcg agatggaaaa
     3421 ttatcttgct cttgttgcca ttatcttaaa agcagtttgc ggtttagcaa gtttagaggg
     3481 ggcattcaga tgcagcaaga taaaacaagc aatatcaacg aaaaaaaaat cacgctttgt
     3541 tcacttattc cagggatggg gcagtttgta aatggccaag cttttaaagg tatcgttttt
     3601 ttcgccatat tcgtcctatt cattggtcag atgcttctgg gcggatttca tgcattggta
     3661 ggattggtta ctttgggaag tataccaatg gaagatcatt cattatttat tttgattgaa
     3721 ggcactttgc aattcatcat tacaatcatt ttcttgattt tttacggttt gaatctttta
     3781 gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa aaatcaatcg gacgatgaag
     3841 gaagtcatac ataatatagg tgatgaaggt tttccttatc tattgacact tcccgcttat
     3901 cttctcatga tttttacgat cattttccca gtcctagtta cattgttcac tgcatttact
     3961 aattatgact tcaatcatat tccaccagcc agtttaattg attgggtagg ggctaaaaac
     4021 ttttttagta tcttcttttt aagctcatat cgtaatactt tttttgcagt cttttcgtgg
     4081 accgtgattt ggactttatg tgcaacaact ttgcaaatca cccttggtat tctcacagcg
     4141 gttgttgctc accagaaatt tattaaattc aaacgtattt tcggtatcat tttcttatta
     4201 ccatgggcgg tcccagcttt tattactatc atgagttttt cgaatatatt caacgatagc
     4261 gtaggtgcca tcaatactca agtgattcct ttcataaacc agcttccttt tgtagatatc
     4321 agtgcaatct cttggaaaac ggacccgaac tggacgaaag tagcaattat cttagtgcag
     4381 ggctggttag gatttcccta tatctatgtg atggttacag gaatcttaca atcgatttca
     4441 gaagacttat atgaagcagc aaaaatcgac ggagcaaatg catggcagcg cttttcaaac
     4501 atcacgcttc ctgctgtctt cctagtggca gcgccaacat ttattacaca gtatacgggg
     4561 aatttcaata atttctccat gatctatctg tttaacaatg gtggtcccgg aagcgtaggc
     4621 ggaggagctg gttctactga tattcttatt tcctggatct ataaacttac gactggaact
     4681 tctccgcagt attctatggc ttcagcaatc acgttgatta tctccgtcat cgttatctgt
     4741 gtgtcattgc tggtatttaa aaagacaaat gcgttcaata tgggggatta acaatgaaaa
     4801 aatttcattc gagtgctcga ttccaaaaac gactttctca cttttttact tacttttttc
     4861 ttttaatact gtcagtcatt atcatctatc cgcttttgat cacagcaagt actgccttca
     4921 agtcaggaaa tattgcagct tttagtttag atctttcaag tgactggact ttaggaaact
     4981 ttactcggct attcagtgaa acactttacg ggacttggta taaaaataca ttgatcattt
     5041 ctgtcctaac aatgatcgta caagtgactg tagtaacttt ggcgggctat acttatagtc
     5101 gctatcgttt tatcggccgc aaatcaagtt tgatgttctt tttagtgatt cagatggtac
     5161 cgactatggc agcattgact gccttttatg tcatggcttt cttattagaa gcgctcgata
     5221 agtactggtt cttgacaatg atctatatag gcggtggaat tccaatgaat acttggctga
     5281 tgaaaggata ctttgacact gtaccaatag atttagatga atctgctaaa cttgatgggg
     5341 cagggcattt caggattttt ttccagattg tattaccgct tgtcaaaccg atgctcgccg
     5401 ttcaagcatt atgggctttt atgggacctt ttggggattt catgttagct aagttcctgc
     5461 ttcgaacacc tgaaaatcag actgttgctg tcggcttaca aacatttatt tctgatgtga
     5521 caaaccaaag agtctctctt tttgctgctg gggcaatttt gattgcacta ccgatttgtc
     5581 tattattctt ctttttacaa aagaactttg ttaccggcct gacatctggc ggaacaaaag
     5641 gataggagca gagtatcatg caaacatttc cgttaaacta tttttccgca ttacgtacgc
     5701 caactcaagt gtttgcggga cgcaaactat tatcatggcc taaatttttc ttgatatttg
     5761 tatttttagt ttccttgatg gtcatgcctg tgactctttt ttatgccaat cagatccaag
     5821 cgattccatt agaacaattt ctatccgttc attcgctgat cgatgaacaa ggaacccaaa
     5881 aattttctga acttgaatta tctgaaactg ggttacaagc atcgcaacaa acgatagctg
     5941 tgacccctga aattttggta ggtgtgtctt tatctgagaa acaacaatcc gatcatggaa
     6001 cttttattga ttttgagaag gaacaatggg tgatccaaca aaaggataaa tctggtatac
     6061 gccgctatac gatgaattat agcccatctt tccaaccaga ttctgtacgt acgcccgaag
     6121 atttccaacg atttttagaa agagaatttt atgccagtaa tcgtccaaca atcattttat
     6181 cttatagttt aagtcttggt cttgtcctat ttgtcatgac gagtctcatt ttattcgggg
     6241 cttccttttt cttgtggatg acaagaaaaa gccaactttc atccattcat acattcaaag
     6301 agagtgcaaa tttgatgctg aatgttatgg gaataggttc catggcagca gcagttgtag
     6361 ggtttatcca ctttgacttt atcttgatgc taagtgtcca aacaacgata actgtcttgc
     6421 tgcttttatg ggtttttgtc aaaacaagat tcaaagataa gcgtgtcgaa tgacaagttt
     6481 aagcgattta tgcgacaatg aatgtgaaaa aaatgaaaaa taaagtagtt tttgaaaaat
     6541 ttctgaaaaa attaaacttc ttagttgatt cttttctttt ttagtattaa cgtaataggg
     6601 tagttttttt aaggaggaaa atcatgttag agaaaacttt atacaatcaa ttattttcac
     6661 gttctttctc gctccctgtc gaagtaaatt attgggacgg tacaacgaaa caatacggag
     6721 atacggacac tcctccccaa atcaaaatca atatccatga agaaatccca atgaaagaga
     6781 ttacaagtaa cgcttctttg gcactaggag aagcctatat ggatcatcga atcgagatcg
     6841 agggaagtat ccaagcattg atcaacgatg cttatcagca agcaaacagc tttttacgag
     6901 gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag
     6961 aagatatcca tgcgcattat gacttaggta acgactttta taaactttgg ctagatccaa
     7021 ctttgactta ttcttgtgct tatttcaata cacctgaaga tacgttggaa caagcacaaa
     7081 tcaataaagt ccatcatatc ttagacaaac tctttatcaa agaaggcgaa acgttgcttg
     7141 atatcggttg tggctgggga acattgatgt tcactgctac caaagagtat aatgtcaagg
     7201 caactgggat cacactaagt gaagaacaat acgatttgat taccaaaaga ataaaagaag
     7261 aacatttaga agataaatgc cgtgtattat taatggatta ccgcgaacta aaaggcgaaa
     7321 cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag
     7381 gttatttcaa agtggtaaaa gatttattga aaccaaaagg aacagctttg atccatggaa
     7441 tcagccgaca acaaggcggt gcgacaaatg cttggatcaa taaatatatt ttccctggcg
     7501 gttatgtccc tggtgttact gaattagttg ataatattac aaaaaacgat ttgcaactga
     7561 ttgatttgga aagcttacgt cgtgactacc aattgacatt agaacattgg acaaagaatt
     7621 ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga ttccttctat cgaatgtggg
     7681 atctatattt acaagcttgt gcagcttcat ttgaatcaag taatatcgat gtgattcaat
     7741 atttgcttgt tcaacctgga aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa
     7801 agtactgttt aaagactatt taaaaagaag ctaagtttta tgatgctcca gaagtgaact
     7861 tttttgtcta acttctagga ttgtctctaa agcttagttt ctttttcttt atattttcat
     7921 aataaaagtt tggttcttta atttttaaa
//
LOCUS       DACCXW010000122         7840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCXW010000122 DACCXW010000000
VERSION     DACCXW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.128"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..107
                     /locus_tag="IVY71_002376"
     CDS             <1..107
                     /locus_tag="IVY71_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAP7986406.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="IVY71_002377"
     CDS             complement(176..904)
                     /locus_tag="IVY71_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7986407.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="IVY71_002378"
     CDS             complement(917..2197)
                     /locus_tag="IVY71_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7986408.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="IVY71_002379"
     CDS             complement(2338..2940)
                     /locus_tag="IVY71_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7986409.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="IVY71_002380"
     CDS             3185..3391
                     /locus_tag="IVY71_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986410.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="IVY71_002381"
     CDS             3703..4422
                     /locus_tag="IVY71_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7986411.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="IVY71_002382"
     CDS             complement(4503..5186)
                     /locus_tag="IVY71_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7986412.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="IVY71_002383"
     CDS             5405..7591
                     /locus_tag="IVY71_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7986413.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2820 a         1316 c         1472 g         2232 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat caaaatagag
     7801 aatatataga aaaattctca ttattcaaac gtaaataatc
//
LOCUS       DACCXW010000123         7771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCXW010000123 DACCXW010000000
VERSION     DACCXW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.129"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(79..151)
                     /locus_tag="IVY71_002384"
     tRNA            complement(79..151)
                     /locus_tag="IVY71_002384"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="IVY71_002385"
     CDS             532..1101
                     /locus_tag="IVY71_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7986414.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="IVY71_002386"
     CDS             1088..1948
                     /locus_tag="IVY71_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7986415.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /locus_tag="IVY71_002387"
     CDS             2138..2842
                     /locus_tag="IVY71_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7986416.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="IVY71_002388"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="IVY71_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7986417.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /locus_tag="IVY71_002389"
     CDS             4679..5995
                     /locus_tag="IVY71_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986418.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="IVY71_002390"
     CDS             5996..6865
                     /locus_tag="IVY71_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986419.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="IVY71_002391"
     CDS             6920..7729
                     /locus_tag="IVY71_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7986420.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
BASE COUNT         2654 a         1317 c         1696 g         2104 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat c
//
LOCUS       DACCXW010000124         7529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCXW010000124 DACCXW010000000
VERSION     DACCXW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.130"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            142..1533
                     /locus_tag="IVY71_002392"
     CDS             142..1533
                     /locus_tag="IVY71_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7986421.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1606..2253)
                     /locus_tag="IVY71_002393"
     CDS             complement(1606..2253)
                     /locus_tag="IVY71_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7986422.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2261..2947)
                     /locus_tag="IVY71_002394"
     CDS             complement(2261..2947)
                     /locus_tag="IVY71_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986423.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2953..4026)
                     /locus_tag="IVY71_002395"
     CDS             complement(2953..4026)
                     /locus_tag="IVY71_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7986424.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4248..4922
                     /locus_tag="IVY71_002396"
     CDS             4248..4922
                     /locus_tag="IVY71_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7986425.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4927..6267
                     /locus_tag="IVY71_002397"
     CDS             4927..6267
                     /locus_tag="IVY71_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7986426.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6416..7402)
                     /locus_tag="IVY71_002398"
     CDS             complement(6416..7402)
                     /locus_tag="IVY71_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7986427.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2340 a         1388 c         1321 g         2480 t
ORIGIN      
        1 ctaaagggaa tcacaccaat tttcatacca attggtttca tttatgaagt tgattccttc
       61 gattaatgag cgtttcattg aaagaaaagg gttgagggag aaaaacatat gatcaggttg
      121 ttggaattaa actatagaaa gatggaaaaa atcattcgaa ttctgtccat ttctgacaag
      181 tggatgacta aaaaagaact tgccaaacga attggtagtt cagaatctac atttgtaagg
      241 tatatcgaag agatcaaaat ccgctgggga acggatgtaa cgattcaatc ttcacaaaaa
      301 ttggggtatc gtcttgaaca tttgaatgta ccagcttatt taaatgttct gatggaaatg
      361 tcgcaaagtt ctacaaacag tcgtttatta aatgagatta ttgtcaatcc gggtaaaacg
      421 attgagtatt attgtgaaaa aatttccatc agccggtctt cttttacacg taagttgaaa
      481 caatgcaatc aaattttaga aagctattta ttaaaagtga tcgttgacca aggttttcag
      541 ttaactagcc ttaaaagtga gctccagttg cgtatctttg ttaccttttt ctttttaagt
      601 tactacggaa gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa agaaattatg
      661 gtcaagaata attgtcagat gaatttgttt caggaagaaa gcccatatga gcaatctttc
      721 tttattatgt attttgttgt gcatctcatg agagaatccc aaggaactcc atttgaatgg
      781 aaagctggag actcttatgt acagttgata aatatcgata aaaaagactt cttaatcttg
      841 aaaaatctct taggaaaatt gaagtacagc agctatataa aagtagtgaa ctattttgtc
      901 aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga gcagatacga
      961 aaaaatatgt cctactgttc gttttcatcc gctgattgca aagaaaaaaa cgaagaatat
     1021 gttgttcaat tattaacgca aatttatttt ttctcccgta ttttcccata cgatacgact
     1081 catttgacga tccgtgcaac ttactttgca cagcagatgc aacaccgcca gatacgtcta
     1141 tatgctggat ttcgtgcaga acttcaattt tattccactc tactgaatca gaatctgatt
     1201 tattatttat ctagctatat cttttggatg cttatcggta atcctgaaat tcaccattat
     1261 acttctgata aaaaaatact gattatcagc gacttaagtc ttcgacatag tcaatatatt
     1321 gaagaatata tcagtgatat tcttgctgtg cataaaattc actctgagac aactgctatt
     1381 actgaagatc agctgtcgaa atacaatctt ctcgagtatg atctgatcgt tacgaatcaa
     1441 cccattttga atgcccatgt tcctcatata ttgattgatg atagtgtgtc attcgcaaat
     1501 gaagaagaac tcatccgctt attcgagtta tgaataatca aaaaacagct atgtataaac
     1561 caccctgata aaaatttcag gacaaataca catagctgtt tctttttatt gtattccttc
     1621 agcaatccaa cgagatttat agcgaaagtc ttttaacaac tcttcttttg tccaattatt
     1681 tgcaatgaga tccgtcaaag tgttcattac gagggtcatg gaaaagtata ggaaactaac
     1741 tgcttcttcc gacgattgaa tggagaaatg attgattctt aataattcca gccatttttc
     1801 gttcattgga gaatcagccg atagttctct acgtttggtt gtctgctgat catttcgctg
     1861 ggttaacaag tataaccttt gccaatagat actttcgtga tctaagtcgc agcaccactc
     1921 taaatatttt tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga attttaagat
     1981 atcttgatgg actatgtgag aactgctttt gatcgtatac aaataagcat cttccaaatc
     2041 ttgaaaatat ttataaaaag caccgcgcga catccccatc ttgtcaacga tttctgagac
     2101 tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt aataggtcat cgattctctg
     2161 tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc atccacatct ctcctttatc
     2221 tctgattata cgtggatagc ccttcattgt catgaaggat tcattccttc tttttctctt
     2281 cttctgtcaa gtacccatct tccattcgat aaacgcgatc actccattga atcatacgga
     2341 catcatgagt aaccataata gtcgcttttt gtttctcatg agcttcttta actagtaatt
     2401 tgacgacttc atatgcatga tctgtatcca aactagctgt tggttcgtct gctaagatca
     2461 agcttggctc attatataaa gcgcgagcaa ttgccactct ctgtctttct ccaccagaaa
     2521 ggtctttagg ataactattt ttaagtgact ggatatctaa agattgcagc aaggaatcga
     2581 tttttgttga atcttctttt tttgaagtaa cttggtcaac tagttcaaat tgttccttta
     2641 ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa tcctatttct ttaaagcgta
     2701 aatctgctcg ttttttttct ggaagctcag ttagtgagtg accgttgatc actatttctc
     2761 cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa agtactttta cctgaaccag
     2821 aaggaccaat gatgctgacg aattctcctt cctttacata aaaatcaatt gctttcaaag
     2881 ctgagacctt tgtatgacca gaaccaaatg ttttggtaac ttttttcatc gttaatattt
     2941 ttttcatttt tattaacctc ctattgctgt aattgggtct actttggtta tcgtacgtat
     3001 cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc ccactatata gcaaccattg
     3061 tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc agactagtca aataagcaaa
     3121 caaaagagca agcagaacgc ctaccacccc aacaataaag gactgtgcta ctaaagattt
     3181 agcgatgaag aaatttttga tcccttgtgc tttcattact ccaaaaatag cagtcttctg
     3241 aagtgtcatc acgtacatga aaataccgac cacggcagca accactaaga acaaaaagta
     3301 tatcatcgca tttaaggtca tattttgggc agaatagcca ggaatacttt caatgaatgt
     3361 cccaatagtt aataactgta gacccttcga tgctttttct ttcgaaattt tagctgaatc
     3421 ctttattacg atcccattga tcggctgttc ttcttcagaa gcaaacggct gttgtccata
     3481 cttcaaagca gtccacgtat ctagggacgc atagatgacg ggagaaactg tgtaataagt
     3541 ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt tgatcgtatc
     3601 tccaatttta tagcctgaat cagccagatt ttgagagatg atgatttcat tctttgtttt
     3661 aaattcatgc cctttcgtga gttttggtaa taaaaatgct tctttcgttg tgccgaaaac
     3721 tgtaacattt tctttgtttt ttcctttgat tgctccgctg tacaatccga ttggtgcttt
     3781 ctctcctcct tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg caaatacttg
     3841 atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg cttttttaaa
     3901 ctcttctgaa agtccattcg ctagtcctga caacatgaat acgacatacg caatcaagaa
     3961 cataataccg acgatcaagc catatcgtaa tttagaatag cgaatttctt tgatagctag
     4021 aaacatttgt ttttcctccc tatgatagat tgatagtgac aaaatgtcac ttgttgaata
     4081 gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt tttcttatca ataaattgtc
     4141 taagacatct ttcgataaaa ccttaacatg agtcataaaa aatttattta agccttattt
     4201 atgctttcca tgataaacta aagtgaccaa atcaatgagg tgggaaaatg attaaactat
     4261 tagtagtaga agacgataaa gtattatcca ataatataaa agaaatactc gctgcaatcg
     4321 gcgagataac acaagtttat gatggtagtg aaggactgtt cgagttggaa agtggagttt
     4381 atgatcttgc aatcttggat ttgatgatgc cggaaatgaa tggatatgaa gtgttagcaa
     4441 aaatacggaa agaaaaaata cagactccag tattgatttt gactgcaaaa gatagtttgg
     4501 aagataagat tgaaggattt gaacgaggtg cagatgatta tctaacaaaa cctttctatc
     4561 gagaagaact gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt ctctttgatg
     4621 aaaatgttct gaagtataag gatatcagtt gtgatttaaa aagcaatgaa gtcttgtata
     4681 aagaacaagt tttgcccata caaggaaaag agtttgaatt gctcgtttat tttttacaaa
     4741 ataagggaat cattttgaca aaagagcaga tatttgaccg gatctgggga tttgattctg
     4801 atacgacgat tacagttatt gaagtttata tgagcaactt acgaaaacat ctgcgaagta
     4861 ctggattgga taaagaaatc aaaaccttac gtaatgtcgg ttatatactg caaagggtgt
     4921 gatgcaatga atggagatat tacacgaaag caacgtagtc gcttttttgc gacaaatgta
     4981 gcagcatttg cttttatctt ttttcttttg ggattcatta ctttacagat attaaaccaa
     5041 tctgcataca gagaaacaga tctgtcatta gagaaaataa attttgattc tcgaacagta
     5101 caattagaga tacaaagata tcagcaaggc gatccctttt taaacccgac tcaaacggaa
     5161 caaaacagaa caaacatgga acagccatta atggaaccat caaatgaccg ttttaacact
     5221 caagtgatct tgtggtctaa aaatggtgag atattgaata aagcagctat tggtgggcgt
     5281 ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc tgaataccat ccaaaatatt
     5341 gaattaaatg aagacagtgg ctcatctgcc ctctctttcc gttcgattac gaaaaaagcg
     5401 gagacgaatc aagatggcgt tgcctacatc caaattttag aaaatacaaa tcaaatcaag
     5461 aattcattag cgacttttcg cacgattatg attttgtgta tgattgtttt ttggattctc
     5521 tctattggta tcagttatta cttgtctagt ctaagcatgc gccctattat tctttcttgg
     5581 agaagacaaa aagaatttgt tgagaatgct tctcatgaat tgcgcacacc gttaacgatt
     5641 attcaaaata gtttggaaca tctttttacc aagcctaatc atactattat tgaagaatcc
     5701 gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca ctggattgac ttcggatttg
     5761 ctgacaatcg ctcggaatga ttctgatcag cagctgcttt caaagcaaat gatcaataca
     5821 caagaatata tcgaacaact tgttaaacca tttcaagaga tggccatcat agatggaaaa
     5881 gagtttattc tccataacga tgcaacatta gaagtaccaa ttgatgaaaa aaaaatccat
     5941 caagttttgg ttattttgct ggataatgca ttaaaatata cgaatccaga agataagatc
     6001 attttggatt catctgttaa ccataaagaa tggctgattc acgtaaagaa tactggccct
     6061 tctatcagtg aagaagatca agcaaggatt tttgatcgat tttatcggga agatcaatct
     6121 cgggcgaaag agactggcgg atacgggctt ggcttggcaa ttgcaaaaca ggtaatcgaa
     6181 cagcatcatg gacagatttc tgtatccaat tatcttccac aaggagtcga ttttaaagtc
     6241 caattgccgt tgaagcctaa aaaataatgc ttatccctaa aaaagggctg tgacaaaaat
     6301 cttgtcacag cccttttttg ttgcgaataa acggtgttcc agaagtagct tcttcggaaa
     6361 taagccgaaa tttctttaag ctgaccactt ctgtcacaac ctctttagct tacgcttacg
     6421 attttttatt gcggtcttca atgtgtacat caccgcttgt gtcaatatcc aattcttctc
     6481 ggctaacatc ttctgtcact ttttcaacat cttcatgttc ttctttatga atcccaactt
     6541 cttcacgaat aactgtatgt ttgcttacat ctacttgctc ttctttgatt gggatagtaa
     6601 tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt actggtttgc
     6661 gttcgatgac gatttcttca cgtttgaccg gtacttctac cgtttcagta tcgttgacca
     6721 catgtttgtg gatgtcgact tctccagttg tcacatcatg tgtatttaca tctaattttt
     6781 cttcttttag ttgaattgta tcatcatcta gatttggttt gcgatcgatg tcttcagtct
     6841 tgcttgtacc cattgtatca gtctcgccag gtacgtccat cggatcagtt cctggtgtca
     6901 tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc attggatctg
     6961 ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc tgggctaaca gtatcttgtg
     7021 tatccattgt atcttctgtt gtttctggct gacgatagcc ttttactgtg atgacaaatt
     7081 ttccatcaga aatatctcct ctatattgat ataaaggatc attttctggt gtagccgtct
     7141 cagaatcata ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa gaacggtctt
     7201 cttcaccatc catccgattt tcatctgttt caacatctat accagacaat ccgtcaaatc
     7261 ctagtctaga tgctgcttca tcagtagtat aaataacgat atcatctcgt tcataccctt
     7321 cgttacgcaa ccgttcaatt actgataatg cttcttccct cgtactatag cttccgatga
     7381 ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa atagatttat gattggtata
     7441 ttgttactta taaatttatt ctataggaga cgattgtttt ctacaaatat ttagattgga
     7501 atttgattaa aacaatttaa ttgaaattt
//
LOCUS       DACCXW010000125         7405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCXW010000125 DACCXW010000000
VERSION     DACCXW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.131"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..250)
                     /locus_tag="IVY71_002399"
     CDS             complement(<1..250)
                     /locus_tag="IVY71_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986428.1"
                     /translation="MDKKATMKRIVELTKSESWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARS"
     gene            complement(253..414)
                     /locus_tag="IVY71_002400"
     CDS             complement(253..414)
                     /locus_tag="IVY71_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7986429.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(429..788)
                     /locus_tag="IVY71_002401"
     CDS             complement(429..788)
                     /locus_tag="IVY71_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP7986430.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(785..1636)
                     /locus_tag="IVY71_002402"
     CDS             complement(785..1636)
                     /locus_tag="IVY71_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7986431.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1653..2483)
                     /locus_tag="IVY71_002403"
     CDS             complement(1653..2483)
                     /locus_tag="IVY71_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7986432.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(2486..3172)
                     /locus_tag="IVY71_002404"
     CDS             complement(2486..3172)
                     /locus_tag="IVY71_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986433.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(3178..3417)
                     /locus_tag="IVY71_002405"
     CDS             complement(3178..3417)
                     /locus_tag="IVY71_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986434.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            complement(3743..3931)
                     /locus_tag="IVY71_002406"
     CDS             complement(3743..3931)
                     /locus_tag="IVY71_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986435.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(3952..4236)
                     /locus_tag="IVY71_002407"
     CDS             complement(3952..4236)
                     /locus_tag="IVY71_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986436.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(4238..4432)
                     /locus_tag="IVY71_002408"
     CDS             complement(4238..4432)
                     /locus_tag="IVY71_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986437.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            complement(4429..5205)
                     /locus_tag="IVY71_002409"
     CDS             complement(4429..5205)
                     /locus_tag="IVY71_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7986438.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            complement(5332..5553)
                     /locus_tag="IVY71_002410"
     CDS             complement(5332..5553)
                     /locus_tag="IVY71_002410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986439.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            5854..6198
                     /locus_tag="IVY71_002411"
     CDS             5854..6198
                     /locus_tag="IVY71_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986440.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            6234..6629
                     /locus_tag="IVY71_002412"
     CDS             6234..6629
                     /locus_tag="IVY71_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7986441.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            6682..6993
                     /locus_tag="IVY71_002413"
     CDS             6682..6993
                     /locus_tag="IVY71_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986442.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            7005..>7405
                     /locus_tag="IVY71_002414"
     CDS             7005..>7405
                     /locus_tag="IVY71_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986443.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYP"
BASE COUNT         2132 a         1404 c         1196 g         2673 t
ORIGIN      
        1 agctcctagc tctatcccag ataatgtttt tgcttaatcc agtaatttca gataactgtt
       61 cagctgtacc tgttactaga attcgatcac catgccagat tgcaattttt ctcggcgttt
      121 tccgtttagg cttttcagtc cacattgatt taccgagctt ttggacttct gcaactattt
      181 ctttgtcttc ttgccaagat tctgacttgg ttaattcaac aattcgtttc attgttgctt
      241 tcttatccac gtttattcct ccaatctacg aatttccctt cttaaattct ctatgtgcaa
      301 atcgattgcc tttctcgccg tttcattgac catcactgcc tttgttcgtt ctagatcgtc
      361 aatttcacgc tgaatgtttc gaatacgcat ttgaatcact tcttctgttg tcatgatgga
      421 ccacctcgtt aaaaacgctc ttccttgaac gtattccgat attttttagc taatatcaac
      481 ggcgcttgat attgatgaca gaacaacttt gccttgatct taaagtcttt tgtctgcatt
      541 cctttgacat ctacgacttt gacaagtttg ccgtttttat aaaatgtgaa gtcgggaata
      601 tactcgatct tgcgatactt ctttccgtct agttcaaatt tcggcatcag ctcaaatctt
      661 tcctgaagtt ttactttcca gccatttgct tcagcttgcc acaaggctag atcgtaatac
      721 tctgcttctg cgatagaatc aaacttgata cctcgatgaa cagttttttt attacggtat
      781 ttattcatgc gatactacct ttcactggtt ttatgcgctt gtctgctgtt tgttggaatt
      841 tcagcgcata accttctgaa ttcttaaata tcctagaaac aattctttca ccgtaggctt
      901 ctcttagttc aggaccagat aagtttgttg tgatgatcgt tgccttgttc tgtctggctt
      961 ctaagagcgt gtttaacgtg ttgtttgtaa actgcctact atttgatacc ccactaccta
     1021 attcagctcc aatatcgtca aaaaccacca aatcagttgt tttaatatcg gctataagcg
     1081 atccttcaat ttcttttctc agttcagtat tgttataaga aaactttatt tgctctaata
     1141 attcttgata gcttataaaa agtattttct tgtcataatt tgagcgctca agtatttccc
     1201 aagctgtggc cattgataag tggctttttc cgcttcctga tttccctgat agaatgaaat
     1261 gtgcaggatg gttcagcaga acctcattcg tatagctttt tgctttttct aaagcgattt
     1321 ttgtttcttg atccactacg tgataatttt ccattttgca tttaaacaaa gttttatctg
     1381 ttaataccga accattttga aaaaaactca acgctcgtgc ttttaagcta tcgttatata
     1441 tccgttcggt ctgtatatcc tctttcacac gtaacgcttt ataaccacaa ctcatgcatg
     1501 ttggtttaca acgttctgaa ccatccttat ttttagctcg ccaactatac aaaggttcgc
     1561 tacattctgg acattttccg ctttgcacta atactcttct tattagtttc tccatagcat
     1621 ttgctaggct ttccatgtga tgcatctcct ttttaaattg gcaagtcgtc atattcacta
     1681 ggattgctgt actgtagttt ttgactttgc tttttatgat tcttcttgtc tgctttgatt
     1741 tcgaatttga gcttctcaaa tttttctctc aatttcttag cacttctaat atttccaaac
     1801 caaaattcat ttgtaggtag ccaattgatc acatactcaa tcgcttctat agatgcttta
     1861 tctctttctt ccatcaacct gattgtgtct gcccattttt cgatatctac tttgttcatt
     1921 tcttttggaa aatcttcagt taaattactt tgcaactttt tagcaaggcg taagtgttcg
     1981 tcagaatact tacctttctt ttcttcttta tctatatctt tatcttcttc tatatcttta
     2041 tctgtaccgt cacgtgacgt cacgctaacg tcattttcca atttgagacg ttcctgtctc
     2101 tttctttccc tgtatttacg gtttctttca gcatttttta gccttacttt atccataccc
     2161 tcgatatttt gatgtttttc ccaattactg atggcaatta gtccatcact gcttagatca
     2221 atcatgttga aatttgccaa tgtagttagc gctaagcgaa ccgtatttac gtttttgcca
     2281 aacaatgtag caagcatttc ttcggtataa ggcatgttcc tctggatata tatcagacca
     2341 tcgtcgttag tctttcctgc taaaactagt aatcgaatcc atataacgat gatggcatcc
     2401 gactcaggaa cagcttggat taatcgtatt ttttcatcgt caaacatagt agttttaagt
     2461 ttgatccaac ttatctcagc caaatttatc ctcctatcct taacttttta attgtttcct
     2521 ggtttaactt gatccctttg atttgatatt tatttttgaa attgatcaca cctattttgt
     2581 gtttctctgt gtgatggatt ctgcagagtg ctgcaaatgt gtactctgaa tgatcaactt
     2641 ctttgcgctt tcgtcttccc agcgctttgt caaagtgatc gatatcagct cctgttttgc
     2701 cacagatgca gcagactctt tttgtaatgc atttgtagaa gtaatactct tgattcgctg
     2761 gtaaaatctc atatccttct ttgaaaggaa tatgatgttc aaagatgaaa tctaggatga
     2821 tattcgctaa gatattggca tcactcacgg ttgtattcga ttcgtctttg aggcttattt
     2881 cgcgccctgt gacaccttca aaacggaagt agaagaattc cttccagaag tccgttggca
     2941 tgcctgtatc gatgaaaata tcgcctatga gcgcatagat gaagtttctt tgctgtactg
     3001 tgaaccgtcg tggatcaata aagcgaattt caatgactcg atcgccatca tatccgtcat
     3061 acatcgtttt cagccgttcg atatttactt cttcgttgat tgttgcacca atgtcgttac
     3121 ctttaaattt ctttagtacc gccgagtatg aatctattag tggtttaaac actcatatca
     3181 cttctctttt gtttcttctc tgtactgatc ttcaagccaa ttaacgcctc gttttagaat
     3241 gcccaagtct ctcttggtcc atttactgtc atcagcggtt atggaagctg catcagtcaa
     3301 tgcaacaatt gcttcatcaa ttgatttttc gtacttgtta gcaaccagtt gtaaattcga
     3361 tttgccccaa tcaggatcgg ccaaatattg atctagagtg gaaagttcat tttccatatg
     3421 ttaaaatctc cttagttatg attttttgag tgactcattg ctttggtcgg cggagtcact
     3481 tttttatttg ttgccatgct tttttctttt cgatatgttg cttgcttaaa ataatagaac
     3541 ggctatttgc ccaccaatta tcagcaatca ctttaccgat ttttagcgct tcttctcgtg
     3601 ccatagttgc tcctttcttt tgagtcaagc agattgatta aaaccatcaa tgctgcgaac
     3661 aagcttcctc cgataatact ttggtgcgct acaatcacta ataaccccaa aatgaatcct
     3721 ataaaaagtg tgtctgtctt cttcataatc taatctccct attttttatt tctagcattc
     3781 tcaaatcctc aagttcagaa gcaattagtt cagcttgtct atctgatagc tcatcggatt
     3841 ttctaagcgc tgcacgatca tcttgtaatt gttttctacg ctgtttaatc agacctagaa
     3901 tttgatgttc ttgttgcaat gtgtaggaca taaaatcatt ctcctttgcc tttagaactc
     3961 aaagttttct ttcaaaaatc tttggagttc cgatcgttca attctgatgt ctaacttgct
     4021 ccactgctgt gtttttaagc ctaggcttat ccaatgtgtt aatttgtcat caccaatgcc
     4081 taaaactttt tttacctctg atttgtttgg atatggagga agctctactg atttgttcat
     4141 aaggtgtaat cgttcgtcca atgaattaag aacagcattc gtaatctgtg tagttaattc
     4201 ggaaactact aaattatctg gaattgttat ttgcatgtta tttctccttc tctaactctg
     4261 ctagtgctgc ctcaattggc ttgatttgtt tatctggttt tctacgccca ttcataatgt
     4321 ccgacatata tggtctagaa attccgatag tatcagctaa ccagttctga gactttccgt
     4381 attttgctaa ttgaattctg actttcaaaa taaattcttg tgacatcgtc attcctcctc
     4441 taataaatcg atttctggaa tatatccttc ttttttcagc gactcataaa tgaacagacg
     4501 ccctttttga gtccattttg tattcatcac aacttttgtt ccgccatcag atttcggaat
     4561 ctcagttgta tgagattttg tgtaaccctg tctcatgtgt tttttgcata ataaccattg
     4621 gttgcctact tttttctgaa tacctagctt atgaagtagt ttgttcatct gttgtggaga
     4681 catcccataa tcagccgcga tttgactaat agttactgaa tctgtagaag ataagatact
     4741 atccaaatac gaaattttgg gttcgtattc tgcaattttt tgttctgcga ttagtcttcc
     4801 agtacgttct tctttcaact tagttgctaa ttggatgatt gtatctggat taagcaaagc
     4861 ttcttctact ttttctggag ttagataacc tccatgtttt ctaattgctg gcaacacttc
     4921 gctagtcacc caacgtttaa atttcttagc atttggttgt ttgcttccta aaattaagga
     4981 gtacaaacct gattcgttaa taatagttaa ccctctaggc gattcaaaag taccgttttg
     5041 gtagttttgc cgatcttcta catcgacgtg tctgttaata tctctactac cattttggta
     5101 ccctagaact ttagccacat cttttccaac aaaaaatggt tcatcatgta ttgttactgt
     5161 tcttacttca tgttgttcaa agttaaaaat ttgcggtgtg ttcatttttg ctcattcctt
     5221 tctttgatat aattttgaat agaaagcgag gtgaaaatag tatggaagaa tttaatatgg
     5281 atgtcgacgc cttattcaaa caaaccgtat tcaagactgt taataaagaa ttcaaaatag
     5341 acttccaaaa tgacgaatct tttccaacag aaattgaatt attcgaagaa ctttctcaga
     5401 gcgtttctga gtctttttct cgtcagttgc aaaagaattt ttttgatgct cttgtggatg
     5461 aatttcatca acaacagcac taaacttttg gtctttaaaa tttaaagtta ttaaagctcc
     5521 ttttgcggaa ggagtttttt tattttgttt catgatgttt cctccttttc tttaaatatg
     5581 taagctaata aaattagcta atttttattg acatttctat ccaactgtct tatatttata
     5641 tacagttaaa taagcctaga aaaaccctaa aaattaacat tcttagtttg ccgacctcga
     5701 attcgtttat tttattaggt gtatttcttg ttgcttgtta gcttattaaa ttagcttacg
     5761 agttaaatat aagacaattg gatagatttg tcaactacct ttctatcttt ttgtataaat
     5821 tattatcgta cgctctagga aggttgattt gacatgcttt tcgaaagggt taaagagctc
     5881 tgtaaaaaaa gaggtatttc tatttctgaa ttagaaaata gaataggatt tggaaaaaac
     5941 tctatttata aatggaagac acaaagtcct aaaatagaaa ctttgcaaaa agttgctgac
     6001 tacttccatg tatcaacaga ttatttgcta ggacgcacag atgatcctaa cgcgggagtt
     6061 gcaccagagg aaagaaaact aaccgtggaa gaagctttag catctgttat gagtagcgac
     6121 ggaaaaccgc tcaccgataa tgatagggaa atactatcag ctatgattga agcatattta
     6181 gagaaaaaag ataactaaat aagtaggtga gtcatttgga cagtcaaatt gaaatgataa
     6241 ttaatgaact cggcgttaag gtagaagagc gtgaaaacct tgatgccgat ggccattatg
     6301 ttgcttgtat gaataccata gtaataaaag ctaatttatc taagtataga agacaaagaa
     6361 ccttattaca tgaattagga cacgcttcta aacatcatga taattatttt ttatataact
     6421 tagcattctc tctccattca aaaatggaat atgaggctga tcgcttcatg attgaaaaat
     6481 tattagatag atatattgca aagtctgaat tagaaccaca caatatcaat tacatgaaat
     6541 ttatagaaga taacaattta agcgttcgct tcgaaccact tgtgaaagaa ttattaaaag
     6601 ctcgcatcta ttgttatgca gctctctaaa attttttaag caaaaaagaa catacgttca
     6661 aaaatagaaa ggtgaacaaa aatgatatat acagaattca aagaatggtt agaaaaaagc
     6721 acaaccggat acgaaacatt tatcatcaaa gctactaatt atcaaattga aaaaaacaaa
     6781 aatagacccc aaaaaaaacg ctgggatgat aagaaaatag ataaagctgt attagaaatg
     6841 tggaaacaag tcgtgactaa cttgtatcaa acaattcgta aagaaaaagg agttccatta
     6901 attaacggga aggaaatatg gcttgaattt atagaggaac aaggactgat cgaatttttc
     6961 aatgatagca tggcagaatt agaatttgaa taggggtaat attgatggca atgataaaac
     7021 aatataaaaa gaaaaatggc gaaaaagcat ggtactttaa aacttatctc ggtattgatc
     7081 cgctaactgg aaagaaaaaa tatactacta aacgaggatt tagaacacaa aaagaagcaa
     7141 aaacagcact ttctaggtta gaactagaat tacaaaaaac aggaatgccc acaagtacaa
     7201 atactacttt caaagaagca gcagaattat ggctagaaag ctacaaaaaa actgtaaaag
     7261 aaagttcata ttcaaggact aaaataatct ttaataaaca tatatatccc aaatttggaa
     7321 atattaagct ttctaaaatt aatacggcat attgtcaaaa ggtagtaaat gattggagtg
     7381 aaaaaggaac ttcaaagcag taccc
//
LOCUS       DACCXW010000126         7390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCXW010000126 DACCXW010000000
VERSION     DACCXW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.132"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="IVY71_002415"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="IVY71_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7986444.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2697)
                     /locus_tag="IVY71_002416"
     CDS             complement(1804..2697)
                     /locus_tag="IVY71_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7986445.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="IVY71_002417"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="IVY71_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7986446.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="IVY71_002418"
     CDS             complement(join(3472..4509,4511..4573))
                     /gene="prfB"
                     /locus_tag="IVY71_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7986447.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="IVY71_002419"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="IVY71_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7986448.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1975 a         1639 c         1289 g         2487 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc
//
LOCUS       DACCXW010000127         7336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCXW010000127 DACCXW010000000
VERSION     DACCXW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.133"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(40..873)
                     /locus_tag="IVY71_002420"
     CDS             complement(40..873)
                     /locus_tag="IVY71_002420"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7986449.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(1065..1769)
                     /locus_tag="IVY71_002421"
     CDS             complement(1065..1769)
                     /locus_tag="IVY71_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7986450.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(1792..2418)
                     /locus_tag="IVY71_002422"
     CDS             complement(1792..2418)
                     /locus_tag="IVY71_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7986451.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            2744..5119
                     /locus_tag="IVY71_002423"
     CDS             2744..5119
                     /locus_tag="IVY71_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7986452.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(5143..5634)
                     /locus_tag="IVY71_002424"
     CDS             complement(5143..5634)
                     /locus_tag="IVY71_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7986453.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            5821..6741
                     /locus_tag="IVY71_002425"
     CDS             5821..6741
                     /locus_tag="IVY71_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7986454.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            6895..7239
                     /locus_tag="IVY71_002426"
     CDS             6895..7239
                     /locus_tag="IVY71_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7986455.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT         2394 a         1324 c         1425 g         2193 t
ORIGIN      
        1 cagaacattt ctcaaataag agaagtgttc tggttttttc tacattgcaa taaaccaata
       61 accaatcaaa atgataccaa ggataatacg ataccagcca aaagcggtaa agtcgttttt
      121 cttcaaatag cttagtaaga atttgatagc taagacggaa accacaaaag atacgaatgt
      181 tcctacaaga agaacaacag tttcttccat tttgaatgaa ttcccttcag ctaaatattt
      241 gacgatcttc aagccgcttg cgccaaacat gacaggaatc cctaagaaaa atgaaaactc
      301 tgtagcgaca aaacgcgaag caccaatcaa gatagatcct aggatcgttg cgccagaacg
      361 ggatgttccg ggaacaagtg ccaagacttg gaataaccca acaagaagag ctgcttggta
      421 cgtaaagtca ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat
      481 aataaaggca ataccgtaaa cgatcaaaac aatggcaact ggtaagaact tgtggaaatt
      541 tgcttctaac caatcatcca aaggcaaacc aatgatgatg gctggtatgc aagcaacgat
      601 cactttaaac caaagtgtcc acgtatcttt tttttctacc gttgatttac ttggagaaaa
      661 tgggtttaac ttgtgaaaat aaagaacgac gacagccaag atcgccccta attggataac
      721 gacattgaac atcgacataa attgtgaact ggcatccagt ttgatgaatt catctgctag
      781 gatcaaatga ccagtactac tgatgggcag ccattccgtt attccttcaa taataccgag
      841 aatcactgct ttgataatat ttgcaaaaaa cataaattcc ttccttccca attaagatac
      901 atcttatagt ataccgttat tttttgagaa acccaatctg ttttttaata ttaagaaaaa
      961 cttgagaaat aaagcagata agtacacgaa gagaaaaaag aagacaaagc cctcgttcag
     1021 actttgcctt ttatgattct ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg
     1081 cgactatgtg ttttcccgtt atcgatataa gacgtatcat tgatgccgtg gatagaaaat
     1141 cgaccgtttg cataagtgac ctttgttacg ctgccattta aaagcccagc acgaacaggc
     1201 tgtttaggat cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa
     1261 acatttccac caccattttt ttcgcagtga tgggcgatat cttcaaatcc tgtccataca
     1321 cggtctcgca atgtttcata agtttccgcc cacccagcgg tatctaagtt gtatacagca
     1381 ttagcaattt gttcaaaaga aacttctgtg gtaaacattt catcatatgt tttaaaattc
     1441 aatacacgag ggatcacacc ccacatttcc atgtcatatc caccttcaag agacccgaaa
     1501 caccattctc taatcctttt ttcaataaaa taaggaattt cttttccctt agaatgttct
     1561 cctagaataa tacgggcagt ttcgattgca cgaccactgt cactagaata agcaaatgaa
     1621 aagtcgacat tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg
     1681 aggggtgtat cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt ttttccgtgt
     1741 cgtacgatat aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt tttaacgatt
     1801 gaaaaaagga tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat gtcctggata
     1861 gactgcaaat tcatcaggta atacaaacag ctgggtccga atactatgaa gtaattgttc
     1921 taaattccct gtatacagat cggttctacc gatacttcct ctaaacaggg cgtcgccaga
     1981 aacgacaaaa tcatcaaaaa taaagctgac gcttcctata gaatgccctg gggtagggac
     2041 gacgcgaaaa gacatgtttc ctaaagtata agttttcatt tcaaattcat attctgcagg
     2101 cgagacaata acatcagcaa tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg
     2161 atcacctagc catttttgct ctagtgggct gacataaact gggatttgat aatacgcgcg
     2221 taaaggttcc actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg
     2281 ctgttggttt gtttttttga tttcttgttt gattcgttcc gcatcttctc cagggtcaat
     2341 gatcaaaagt gcttcttcgt tgtagaccaa ataacagttt tcttcaatca ttcctgtttt
     2401 taatctttcg atatgcatgt gcattcctcc ttgatagcca aaacttcttt catccattag
     2461 tataacgcat tcattgctag gaaacaaaaa caggtactta tttctcttta cttatctgaa
     2521 ttatcagact atatgaaaag tatttggata ttctaaagag aaggaaagta agttttcata
     2581 gcattttaat atgtccttgt tacattagat tcatccaaaa atccaaattc tatatcctat
     2641 atccctgcct aatcagcagg gtgttttttt atgtttggaa agagagctct ttttgctaaa
     2701 ctaggttttt tcaggtaatc ttagagagaa agaggtggtg gaaatgatca aacagtcaaa
     2761 aatatctgtg cggcacttag tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa
     2821 aaaaagtaac catactgcat tagaaggagc gagaatccac cgcaagctcc aaaaagatgc
     2881 gggagaaaac tatgaaaaag aagtcttttt aaaaacaaca gttcaattag atgaatatta
     2941 cctgatcgtt gaaggccgtg cagacggtat tttcaaaaga gacggtattt attatataga
     3001 tgaaatcaaa acctccgaac cacgatttga agatttagaa ccagaacaag tggaactttt
     3061 ctttcatcaa gcaagggtgt atgcctatat ctatagtcaa gaacatgatt tacaagaaat
     3121 aaatctgcaa ttgacttact tccagacggc agaagaagtg atcacgagaa aggttgaaca
     3181 tcagacaaga gagcagttag aaactttttt taaaaagctg actgaagatt ataaagaatg
     3241 gcttgttttt caagaaaact ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt
     3301 cccccatgat gagtatagga aaggtcagcg tgaactagcg gtagctgctt ataaaacgat
     3361 ccgtacgaaa cagaaattgt ttgtagaagc acctaccgga acaggaaaaa caatctctac
     3421 gttattcccc gctttgaaag ctgtagggga agaagaggga gaaaagatct tttatctaac
     3481 ggctaaaaca ataactagac aagtagctga agatgctatg acagcattaa aagatacagg
     3541 agctgaagta aaaagtgtca ctttgacggc gaaagacaag atctgctttt tagatgagac
     3601 gacctgtaat ccagatcagt gcccatatgc caatggttat tacaatcgga ttaatgaagg
     3661 tttatgggat cttttgaatc atgagaatca gatcactcgt gaagtcatcg aaacttatgc
     3721 aagaaagcat acactttgtc ctttcgaact atcattagat gtcagtatct ggtgtgacgt
     3781 aatcattggg gattacaatt atttatttga tccaactgtt tatttaagac gtttttttga
     3841 agatgaaaaa aacgaggact atctgttttt gattgacgaa gcacataatt tggtgaatcg
     3901 ttcccgagag atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc
     3961 tataccaaag gaattcaaaa aacttcatcg ccgtttcaac aaactattaa aagaatttga
     4021 ttcgatccga gaaatagcaa aagaagatca ttgggattat catcatcaaa aagcaccggc
     4081 tgaatcttta gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat ggctggcaga
     4141 atttcctgag cacccatcac aagaacaatt gcttccttat tattttgatt tgcttcattt
     4201 tttgaaggtc agtgaatttt atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg
     4261 cgatctgata gtaaaagagt tttgtattga tccaagcttg tttcttgagc agtcgttgga
     4321 taaaggaaaa agcagtattc ttttttctgc tagtttttca ccactctctt attatcagga
     4381 aacactgggg ggacaaaaaa gtctggctta caaactgccg agtcctttcc cagaaggaaa
     4441 ccagcaagtt ttcatcgcaa attacctaga gacgacttat cgaaaaaggg aacacagtct
     4501 ggcaaaatta gtagagacga ttcatgcttt tgctgatgga aaggtcggga attatttcgt
     4561 tttctttcct tcttatcaat atttagatct tgcggtggaa gcattcagac agcaatatcc
     4621 cgacagcaat gtgctaatcc aagaaacgga catgaatgag gaagaacgcg aacgattttt
     4681 aacaaaattt caaacaaatc ccgctgaaac attgattgga ttttgcgtac ttggcgggat
     4741 tttttcagaa ggaatcgatt tgagagggga ccgattgatc ggcagtatga tcgtaggtgt
     4801 aggtttgccg cagatgaatc atgagcagga actgatcaaa tcatattatg atgagaagga
     4861 acaccttgga tttgcttacg cttatcagct tcctggaatg aacaaagtct tgcaagctgg
     4921 tggtcgagtg attcgtggca tggcggatca aggaatcgtt gtattggcag atcaacgatt
     4981 ttctagtttt tcttatcgac gtttattccc acaacattgg caaacagcaa gagaagtacg
     5041 aagtgttggg gaattggaag aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa
     5101 tcaaaaaaac gaggaatgac agacgtcagc ctcgtttttt gattaacgat agttagtgaa
     5161 ttgcaactcg attggtaagt caagtttacg taataaagca atcacagctt gtagatcatc
     5221 acggttctta cctgtcactc taatctgatc ttcttggatt tgtgctttta ctttcagttt
     5281 ttcatttttg atagctgtag tgattttctt cgcgttttca cgatcgattc cgttgaccaa
     5341 atcggcagtt tgtcgagctt tgccacctag agcgtgttct gattcggaaa aatggatatt
     5401 tttgattggc acatttcttt tgatcagctt accaaataat acatctttga tctgttcgat
     5461 tttgaaatca tcatcgctca atacaactag attgctgttc tctaatttga tttctactgt
     5521 agaatccttg aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa tcgcattttt
     5581 tacttcttct gtattgactt ctgatacaat gtcgaagcta gcatcttttg ccataggaac
     5641 cctcctgata tacatataaa tatttaccat acatctatct tatcatagga ttagggcaga
     5701 ttcttacctg tttgctaaaa ttcttctcat ttgagttcga agtattttat tttaatagat
     5761 aaactgttat tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt
     5821 atgactgcgt atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga
     5881 ttgaccgctg cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg
     5941 atgaatggga tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata
     6001 gacacaggaa catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa
     6061 gtaggaaaac gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg
     6121 atgctgcttc cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt
     6181 gttttattag gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg
     6241 gatatcatct ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg
     6301 attctaaatg gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg
     6361 tatacgatta tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat
     6421 caaaaagtaa cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca
     6481 aaacggatgg tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa
     6541 gggaacatga tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt
     6601 catgaaatcg acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg
     6661 tttgcaaatg aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa
     6721 aatcgttcac aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca
     6781 tctaataaga acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca
     6841 agcagctaaa aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc
     6901 gaagcaaacc aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct
     6961 gccttggaaa tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt
     7021 tatgatatag aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact
     7081 atgggctgtc cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg
     7141 ccagaagtaa cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag
     7201 atgagccgtt atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat
     7261 gagaagcaaa taggaaaaac aaaaaagcct tttatccttt ctagaagaaa agagataaaa
     7321 ggtttctttg tttata
//
LOCUS       DACCXW010000128         6994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCXW010000128 DACCXW010000000
VERSION     DACCXW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.134"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            678..872
                     /locus_tag="IVY71_002427"
     CDS             678..872
                     /locus_tag="IVY71_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986456.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1063..1312
                     /locus_tag="IVY71_002428"
                     /pseudo
     CDS             1063..1312
                     /locus_tag="IVY71_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1556..2386)
                     /locus_tag="IVY71_002429"
     CDS             complement(1556..2386)
                     /locus_tag="IVY71_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP7986457.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2594..3928
                     /locus_tag="IVY71_002430"
     CDS             2594..3928
                     /locus_tag="IVY71_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7986458.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3969..4880
                     /locus_tag="IVY71_002431"
     CDS             3969..4880
                     /locus_tag="IVY71_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7986459.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4877..5731
                     /locus_tag="IVY71_002432"
     CDS             4877..5731
                     /locus_tag="IVY71_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7986460.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            5728..6345
                     /locus_tag="IVY71_002433"
     CDS             5728..6345
                     /locus_tag="IVY71_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7986461.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6347..6865
                     /locus_tag="IVY71_002434"
     CDS             6347..6865
                     /locus_tag="IVY71_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7986462.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2096 a         1205 c         1399 g         2294 t
ORIGIN      
        1 ctaaaggatt cctaaccatc ttattagctt ttatagggta tatgttggtt gggctgctaa
       61 aatcatacag taatgagttg ctgaattttt caacatttat taatgataca ttagttccaa
      121 gtttgttttt cattgtattt tttgctgttg gttattttat aataaagata tgatgactaa
      181 tttagctcaa ctattccagc gtcagctgaa gatattgcaa ttcccgattc cttaatcatt
      241 tatctggatg agacagaaag tgaagttgca acagatgtgc atgtagttga ggataaaaat
      301 gatagtttaa aaattaatag ttcagatact ttactaagag taagtcctat cttttcagct
      361 gttatttaga tgcaagtagc aactaagtta ggtagtactc ctgcaatgga taggtatttg
      421 tgtgttcgat atataagtgg tcgaagtatc aaggttatgt atactggatg ggagatagaa
      481 gagttaaaaa ttggaggtca ccgccacaaa tggaatttag atttagaggt accttagatt
      541 tggtatagtt attaaacaca agatggcctg gtttgtaggg aaagtcgaag agtatttttc
      601 cacttctgcg cccctttaat ttttctagta atgccgtgaa tttgtgatta attggaaata
      661 tgaagaaagg agagtctatg aaaacaacac tagttctatt ctataaaaag catccttatt
      721 ttacacttct gataaatata ttgttggcta gtgttatcgg gatttctgtt gaatatctaa
      781 taaataaaga tttcataggt tcttgttttt atacggcttt atttcttgga cttcttgaag
      841 cattttccat ttataaaaaa tcaaaaaaat aaaatagatt gccgtaagag atctttttca
      901 tttcattttt ttctgaaaac ccgttgtttc tgtcagtgaa gcagcgggtt tttagattta
      961 ttgtctttag acctaattga gcgcgccgag cgattgaaat acaaaaccac ttgctatgcg
     1021 ggtggagcaa taaagggaaa tagtttagcc cgtacaaagt agatgtgtaa ataccacatt
     1081 gtattcacac caaagtatag aagaaaaata atttataatc aatatcgagc caatattcag
     1141 gagatcatca agagattgtg caaatctaaa atataaattt gggtatcgac acttttgggt
     1201 ggaaggatac tatgtgacaa aagtaggaca aaatgaagcg accatcaaaa agtacattca
     1261 agaacaagaa aaacatgata gtgcgttaga taagctgagt gtacgagagt aagacgatcc
     1321 ttttaagggc taaggagtag tacataaacc ttttaagagg tgggcaaaac tggcaaaggc
     1381 aatatggctt gaacataaaa tatgaaagcc agcgtcttta ggcgctggcc ggtgatttga
     1441 gcttatagct ctagagaaaa ccaccctttt gaagggtggt tatgattaat gactttagtc
     1501 agttttgctc acaaagcgtg tggaacaaaa aacgggtgtt tctacgggta gatatttatt
     1561 tcttgaatgg aggtgtccca tacatttttg gtgttactgg ttctaatttt ggtgcagcgc
     1621 cttcgatctt aggttctttc aaccattgat attcgccttc gccatccagt gctttgccac
     1681 tagcccaaga accttcacta ctttggtctc cctctgagaa gttatagaag tcataagcaa
     1741 tgtcttttcg ttctttgtca cgcgggaaag tacttgggac taagattgaa ccttcttttt
     1801 cttctaattc tttcactgca cattcccata gattttgatg atagctatct cttgcaagga
     1861 gaacagacag catatctttt acgccacgat catcgatcat ttcgtaaata cgtgctactt
     1921 gaagtcttcc ttgtgattcg gcatttaaat tagctctaaa gtctgctaac aagttgccac
     1981 tcgcaatgat ataggcaccg ctccatggat tgcccacgct gtcagcagga cgaggtccta
     2041 agccagctac gatcgcgtgc tgtggattca ttccagagat aatggcaccg attgttggat
     2101 ctttttgcat cgctgcttct tgatcttcaa caggcgcacc ctctaataaa cgagcaacca
     2161 ttgtcgccaa catttccacg tggccgattt cttctgttgc aatgtccatc agcatatctt
     2221 tatatttttc ttctcctcgg cagctccagc cttggaacag gtactgcata gctacactca
     2281 tctcgccata ttggccgccg atcagctcct gcagatattt cgcgattaat ggattcggac
     2341 gatctggttt tgcttcaaac tgcaattctt tctggtgtct aaacatcaaa attcctccta
     2401 atctattttt aatacattct cattctgcta cgaaagatta aaaaattcaa agaaagcgct
     2461 cgcgaattat ataaagtatt ggtatcatta ataatttttt tagaatcata ccttataaat
     2521 tgttattttc agaacaaaat gatatcgtta tattaaaacc ggcatcggtt acattaaaat
     2581 ttggaggtaa aatatgaaaa aattattgtt tacagggttg ctggcaagtt cagcattgtt
     2641 tttattagga ggatgcggat caagtaatgc aagcgatgcc agttcaagtg cagcaaattc
     2701 ttctgaaaaa acaagtgatg gaagaaaaaa tattgatttc tggtcctttt ggggatccgg
     2761 tgcacgaaaa gaagctatcg aagaaatcat tgaggatttc aatcaatcac aaaacgagat
     2821 ccatgtaaat tataagtatc agccatgggg tgacatctgg actaaatctt tatctgcaat
     2881 aacagctgga aatgcacccg atgttattgt acaggacata aattccgttg cccaaagagc
     2941 agaagcacag caagcaacta atctctccaa atatgtagaa aaagacactt ctaaggattt
     3001 ttatccgcag ctttgggata cagtagaata tgaaggtgat ccttatgcga tcccattcaa
     3061 tacagataca caagtcattt tttataataa aaagttattt aaagaagctg gaatcagtga
     3121 acaagatcta ccgacaactt gggatgaatt agaagaaaca gcacgtaaac tagataaaaa
     3181 agaaggaaaa gaatttacac gcatcggttt ttatccatta tggaatttag gggctgatgt
     3241 atgggcgcta aacgcagata acgggactag ttggtttgac aaagatgaaa aagtcaagat
     3301 tgatactaaa aataaagtgg aagccttgga gtggatccaa gactggcaag attattatgg
     3361 gaaaaatacg atcaataaat tagaagccga atttgggtct ggtgtatctg atccatttat
     3421 ttccggatta gtagccatgc gtgcccaaaa tatcaattat tactctagtt tgatggaaaa
     3481 tgcaccggat gattttgatt ttggcgtgat gcagctccca gaaaaagaag aaggttcagg
     3541 ccattggtcg tggggtggcg gattcgtctt ggaagtacca tacggagcaa aagatcctga
     3601 ggcctcttat gaattcatca aatatctttc ttctccagaa gtacaagaaa aattcggcga
     3661 aaaaagtttt gatatcatgg cgagcaagac agccaatgag aatttagtga ataatgacaa
     3721 cctaacggat aaaggaaaaa tgatctatca aatggcggac gaaaacttta aaaatacggt
     3781 gatcacacca gttcctttag atgctccgga ttacacgaca ttagtgaatg aacagattga
     3841 ccagattctt ttaggaacaa aatcaccaaa agaaggattg gcagatgcac aaaaagcagt
     3901 agaaaactta gtcaagcaaa atcaataatc catttttgac tgtctaggta cagaggaggc
     3961 aagaaaattt gaagcgttta tctatccgaa agaaaaaaga agcaagatgg gcctatttat
     4021 ttatctcgcc ttttattatc gggttcttgg tatttatgct agggcctatg cttttttcag
     4081 ttattggtag tttcaccgat tataatttga catccaagat gaatttcatt ggtctagcaa
     4141 atttcaaaag aatgttcgtt cacgatgatt tattttggaa atcgttatac aacaccgttt
     4201 atttcgtcat cttcaatgta ccgctgacga cgattttcag tgtgttttta gccacagtac
     4261 tcaatcaaaa aatcaaagga atcagtgtat tccgtacagc tttctattta ccggcagtct
     4321 tatcaggagt agcagtctac atcttatgga tgcagctgct ttctccatct gcaggattga
     4381 tcaacacatt tctgggctgg ttccatatcc ctggaccagc ttggctgttt gatccacaat
     4441 ggacgaaacc tgcgttgatt ttgatgaaaa tatggagttc tggaggagca atgctacttt
     4501 acttagctac attacaaaat gttcctagat ctttatatga atcagcagaa atcgatggag
     4561 caggcgtttg gggaaagttc aaaagtatta ctttgccatt aatcactcct attatttttt
     4621 acgatgtgat cacctctacg atcggttctt tccaaatttt tcaagaagct tatgtcatga
     4681 cgaaaaatgg aacaggcgga cctgcaaatt ctttgctttt ttataatctg catatgtgga
     4741 acaaggcgtt cgtagctttt gatatgggtt atgcgatggc tatgtcaatg atcctgtttc
     4801 tgatcgtgtt ggttctgacg tttgtcaatt tgaagcttgc acctaagtgg gtctattatt
     4861 ctggaggtga cggcaaatga tggattattt caaaaagacc agtgtgaatc ggcaaacaaa
     4921 aaaaatacta agttatgtcc tcatgacagt aataggaatc attttgatca ttccattgct
     4981 ttggatggtg tttacttcat tgaaaccaat ggaagaaatc gttcgttatc ctccgacctt
     5041 tttcccagaa aaaatcgtat gggaaaacta tctggatacg attgccgcct ttcctttttg
     5101 gcgctatgca agaaatacat tattcatcac tgtgctagta gtgatcggta atgtcctttc
     5161 aaattctttt attgcttatg gatttgcgaa gcttgatttt cctgggaaaa agctgatgtt
     5221 tgctttggtt ctttctacga tgatgatccc ggggtttgta acgatgatcc ctcaatatgt
     5281 attattctcc aagatcggct gggtaggtac ttatttgccc ctgatcgttc catcgttctt
     5341 cggcaatgcg ttcaatatat ttctgatgcg tcaattttac ttatcgatca ataatgaatt
     5401 gatcgaagct gctgaaatcg acggagcgaa tcatttgtat atttggagtc atttgatgct
     5461 tccattgacg aaacctgcct tgattaccat tgcgatcaat tccttcaacg cagcttggaa
     5521 tgatttctta ggtcctctgt tatatatcca agatcaagaa aaatacacgt tacagattgg
     5581 cttgcaagtt tttcagaacc aagcaacgac acaatggaat tatctgatgg caggagcaac
     5641 acttgtcttg atcccgacga ttttgctgtt cttttttgca caacgttatt tcattgaagg
     5701 gatggatttg acaggaggtt caaaaggatg atgaatcgat cgattggtaa aaaagccaat
     5761 gattatttct tagaaatcat tgaatggatt ccacgtctta tccatttaca gctactatgg
     5821 ttcctgtgcg tcttgcctgt tttcacatta ggaactgcaa gtcgaacagt tctgtatatg
     5881 atttatcatt acaaaaaaaa tgaagagaaa aagctcggtt cactattctg gaaaaagttt
     5941 cgtgagaact tctctcttta tggaaagcaa gatagtttag caagtgttta ttttcttctt
     6001 ctattgatcg attatcagat tttccgctat tggggtgggg gaattggcta tacattgatg
     6061 tatactacgt tgtttttgct tctattaagc gcgcttctct ttagttacaa aatcttgctt
     6121 caactggaac aaaaagaagc ttcttggatt actgcatttt tcttgttttt taatgatttc
     6181 aaaagcgcgc ttttctattt ggcaggcacg ttgatccttc ttgtcgcatt ctggtttgca
     6241 ggaccaatct attttgcttt attaggtttc agtttcttgt tttattttca agtcctgctt
     6301 tttatccatc gtacgcaaga aaaggttctt aaaaaggagg aataacatgt tgcgtttgat
     6361 tgctaaaatc gatgatagta ttatcaacaa gttcgccaag caacggaaaa cggatagtcg
     6421 aaaaatcaat tttttgaaat ttatcagtaa tatctctaat ggtggtttta tttggttgct
     6481 ccttgctgct gttttagtct tgatacctga tactagagaa tatggtctag ccattcttat
     6541 cggcagtttg atctattttt ttgttggttt gctttttatc aaaaaaacga caagacggaa
     6601 aagaccttat gaaaaaaatc gtcggcttgt cctttacaca agtaaaccga agacttcttc
     6661 ttttccttca ggacatacag cgctagcctt tttatcagta gctgcgttgt tcgaattaag
     6721 tccagttgca ggaatattag cctttttatt tgcttgtttg atcgcttatt cgaggatttt
     6781 tttactttta cattatccta ctgacatttt aggaggagct attttaggaa gtgttattgg
     6841 catcctatgc gctgccgtta tctgaattaa ttgtacgaat aaagcatgag tgcgtaaatg
     6901 cgcatttgtg ctttattttt ttgtttcgag gaaagcgtag ttgtttattc atttgtctta
     6961 ataacttaag ttttgttaaa tgagaacgtt ataa
//
LOCUS       DACCXW010000129         6933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCXW010000129 DACCXW010000000
VERSION     DACCXW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.135"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(60..1613)
                     /locus_tag="IVY71_002435"
     CDS             complement(60..1613)
                     /locus_tag="IVY71_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7986463.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            1761..3050
                     /locus_tag="IVY71_002436"
     CDS             1761..3050
                     /locus_tag="IVY71_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7986464.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3176..4255)
                     /locus_tag="IVY71_002437"
     CDS             complement(3176..4255)
                     /locus_tag="IVY71_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7986465.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4364..4804)
                     /locus_tag="IVY71_002438"
     CDS             complement(4364..4804)
                     /locus_tag="IVY71_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7986466.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4861..5271)
                     /locus_tag="IVY71_002439"
     CDS             complement(4861..5271)
                     /locus_tag="IVY71_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7986467.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5452..5886)
                     /locus_tag="IVY71_002440"
     CDS             complement(5452..5886)
                     /locus_tag="IVY71_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7986468.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(5986..6834)
                     /locus_tag="IVY71_002441"
     CDS             complement(5986..6834)
                     /locus_tag="IVY71_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7986469.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2478 a         1269 c         1150 g         2036 t
ORIGIN      
        1 cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc ttatttggtc
       61 taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc ttttaattca
      121 aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt gatgattcct
      181 tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca gtcgctgata
      241 acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt tgaaaaaatt
      301 tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata gttcacacca
      361 atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc ttcaaccatc
      421 cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg ttctctgaaa
      481 tccatataaa attgttcaat ggcggtttgg acttctttca acacgctatg ttcttgataa
      541 agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc attataaagt
      601 ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat aaacaaatca
      661 aatacagtac aaaaggactc caccatttct cttgaataaa gaacatgagg aacgtttgct
      721 ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt cgtgatagaa
      781 agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag attaggtcgc
      841 aaaatatcta cagagggatc aataaaaaag tcaatatatt catactctgt gaattctttt
      901 tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa tagaaaatat
      961 aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa aaaagtattc
     1021 atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt acttgaaaag
     1081 atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc agaaaaagac
     1141 tttctgccat tattagaaaa ggagatagac aaaccgtaca atgctaaaaa tgcattaatc
     1201 tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact taaactgata
     1261 atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac tgattcgttg
     1321 cagtatcttc caaatagacg ataatagcag taagccggat caattccttc aaaattcaac
     1381 tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg ctcaaagtct
     1441 tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa atgatctgct
     1501 aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat atctaatttt
     1561 gtattgcttt ttttcttcaa gagaaacgtt ttaatcgctt cttgagtttg catgaaagag
     1621 gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc ataaatttag
     1681 acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg catacaaaaa
     1741 aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca tttgcagcct
     1801 taagcttatc gcttactata aatcagacag tccacacaga ggagatagaa acagccaaat
     1861 ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa ttttcatcct
     1921 tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa gcaaaaattg
     1981 actattcagg agacttagca cctatactac aatcagctgt tacatttgag aatgttgatc
     2041 ccgctttgtc gattggctat gacgagaaca ctttgactat ccaacacaag caagcgatat
     2101 ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt actgtcaagg
     2161 tatcagatgg actaccgaac gatcatcatc atttaacgcc gtatagtcag cggcagacaa
     2221 ttgagcaaga ttcagctatc gactcaggag gagatcttgt tgaagaacca acagataaac
     2281 cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac
     2341 cggaaataga acctggagaa gaacccgata cagaaactca accagagcca gacaatgaat
     2401 caaaacctga aataacccct ggagaaaaac cagatgtaga cccagaagaa aaaccggatg
     2461 tgactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
     2521 cagataatga aactgaaaat ccagaaaaac cagaggtagc cccagaagaa aaaccagatg
     2581 taactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
     2641 cagataatga aactgaaaat ccagaaaaac tagaggtaga tccagaagaa aaaccagatg
     2701 tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa
     2761 aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa
     2821 taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa
     2881 ccttaaaaag tagcaaggat acaacctttg ctagtcctga aacgaaaaat aaacagctac
     2941 caaaatccgg agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta
     3001 tagcaacaat gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata
     3061 atgtctgtga acgcattttt caaataaaaa gaaatatcca gaaaacgacc ccaaaatacc
     3121 agattgaaat ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag
     3181 ggatatctgt ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa
     3241 agaaggtaaa gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa
     3301 caagtaagac cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg
     3361 gattcagcac ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa
     3421 catctcggat gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa
     3481 cacctgctac tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat
     3541 gaaagccaaa tacagccaag gcgattgtag tgagcaacgt gtttaaaaga gcgataacaa
     3601 attgagcttc taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat
     3661 cttgaaagaa ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt
     3721 tcttttcaat cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt
     3781 ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa
     3841 aatcgtttcg ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct
     3901 gatgctcgta aaaatcaata acggactgga tcatatcaaa tgtttgatga ataagcatcg
     3961 ggatatactt tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag
     4021 ctaaaacacc tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca
     4081 aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa
     4141 caaaaacgac aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct
     4201 gataaacggt caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat
     4261 gatggaatat gaaaataaga aagagaaaga gcaaaaagaa aaaccgctaa atgacgttat
     4321 cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt
     4381 cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct
     4441 gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg
     4501 cagcaccttg aggagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac
     4561 gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca
     4621 gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca
     4681 tcacaagata ctgggtatag gtaagattca gcggcttgag gagggaagtg tagtgtctaa
     4741 tgatttcctt agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt
     4801 ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt
     4861 ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg
     4921 acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat
     4981 agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac
     5041 ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc
     5101 actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc
     5161 gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgaacttc
     5221 tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc
     5281 acctttaatt atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa
     5341 atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag
     5401 caaacatata caaaaaaata tcagagacaa aaactaatct catcataaac attacatata
     5461 tttctcaaaa agttgttttc tttcttcaaa acttacttca cgatggacct gatgaagcat
     5521 ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt ccatcatttc
     5581 tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg tgtcctcgat
     5641 atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag gagcagtcaa
     5701 accaagagac gtgttttcat ccagcatatg gaatctcgtg ccatacaagg taaaaatgac
     5761 ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat caaagattgt
     5821 ttgatacagt tctagtgctt tcaaactatc atttaataca aaatctattt ccgttccttt
     5881 atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt tattatacca
     5941 gttaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt cgattggatg
     6001 ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa aggcacttgg
     6061 atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca caacgatcaa
     6121 taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg taaggccacg
     6181 tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac tcatgaccat
     6241 aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg aagtgattgc
     6301 aatagataaa atagcataaa gaaactcttc tattccaaaa acaaacagat taagagaaac
     6361 aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat attttttaaa
     6421 aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata aaatcgcaac
     6481 tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg aagtaatcat
     6541 tgtttctgga actattgcaa tgattaaagg aaaaaacaag cttccgaaaa gtactttaaa
     6601 aaagggtttt ttacctaaaa atagaagagc tagtaccaac ataacgatat tcaacactaa
     6661 aataactata gctcggtcaa atcctaaaac agcttccagt aatattccta gaccgctgac
     6721 acctccagca gcaatatgat gaggccctaa aaacatattg atagcgactc ctaatatgaa
     6781 gagggcaaaa ataatcttaa tgtatgtcca tataaattgt ctcgtttctt tcatttatcc
     6841 acttccttaa ttcattaata atactcttat cttattataa cttacttttc accttaagtt
     6901 aactttaatt aataaaaaaa ttaggctagt cta
//
LOCUS       DACCXW010000130         6814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCXW010000130 DACCXW010000000
VERSION     DACCXW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.136"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            60..833
                     /gene="sufC"
                     /locus_tag="IVY71_002442"
     CDS             60..833
                     /gene="sufC"
                     /locus_tag="IVY71_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7986470.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            846..2132
                     /gene="sufD"
                     /locus_tag="IVY71_002443"
     CDS             846..2132
                     /gene="sufD"
                     /locus_tag="IVY71_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7986471.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2129..3364
                     /locus_tag="IVY71_002444"
     CDS             2129..3364
                     /locus_tag="IVY71_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7986472.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3351..3821
                     /locus_tag="IVY71_002445"
     CDS             3351..3821
                     /locus_tag="IVY71_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7986473.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3826..5217
                     /gene="sufB"
                     /locus_tag="IVY71_002446"
     CDS             3826..5217
                     /gene="sufB"
                     /locus_tag="IVY71_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7986474.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5315..6457)
                     /locus_tag="IVY71_002447"
     CDS             complement(5315..6457)
                     /locus_tag="IVY71_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986475.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(6508..>6814)
                     /locus_tag="IVY71_002448"
     CDS             complement(6508..>6814)
                     /locus_tag="IVY71_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7986476.1"
                     /translation="ELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYK
                     DISKYSSVEDFFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT         2163 a         1305 c         1433 g         1913 t
ORIGIN      
        1 cttcattctc aattagtcta aaatgagtga gaacgaaaga gaatttgggg gtataatgca
       61 tgtctgtctt agaaataaaa aatctacatg tgtcgatcga ggaaaaagaa atccttaaag
      121 gcgtaaacct aacaatgaaa accggagaaa tccatgccat catgggacct aacggcacag
      181 gaaaatctac cttatctgcc gctatcatgg gcaatccaaa ctatgaagta acagaaggcg
      241 aaattttgtt tgacgggaaa aatgttttag acttggaagt agatgaacgt gcgcgacttg
      301 gcttattttt agctatgcaa tatccaagtg agataccagg tatcacgaat gcggaattca
      361 tgcgtgcagc gatcaatgcg aaacgtgatg aaaacgacaa aatttctgtt atggcatttt
      421 tgaaaaagtt agatgaaaaa atggctttac taaatatgcc agaagaaatg gctgaacgtt
      481 atctgaacga aggtttttct ggcggagaaa agaaacggaa tgagatcctg caattgttga
      541 tgttagaacc tacatttgca attttagacg aaatcgattc aggtctggat atcgatgctt
      601 tgaaagtcgt tgctaaagga gtaaatgaaa tgcgcggaga caatttcggt gcattgatca
      661 tcacccacta tcaacgatta ttgaactata tcacacctga tgtcgttcac atcatgatgg
      721 aaggccgagt agtcatgaca gggaatgctg acctagctaa acgtctggaa gcagaaggct
      781 acgctggtat cagtaaagaa ttaggaatcg actataaaga agaagaagcg taagggggag
      841 caagaatgaa agaaaaaaac tggctcgatt atcttgacgc ggtaaatgat ttttctttaa
      901 gtaaaggcga acctgattgg atgagaacct tccgtcaaga cgcactagcg aaagcagatg
      961 aactgccatt gccgcatatc gatcgggtga aattccatcg ctggtcgtta tttgatgtga
     1021 aagaaacaca aaccatcagt gaaacaggca cgattccggc atttgatgca atgaaagaca
     1081 atcctgtgct tgtccaacaa ggttcatgga cgatttttga acaactgcct gtggaattag
     1141 cagaaaaagg agtcattttt accgacttgt tcactgcaat gatcgaatat ccagaactag
     1201 tccaagaata ttatatgaaa aaagcagtaa acatgaacga agaccaattg actgcactcc
     1261 atgtagcttt tatgaacagc ggtattttcc tgtatgtgcc taaaaatgtc gtgatcgatg
     1321 aaccactcga gtccttattc attcaagatg gcgcatccga tgagcacttc ttcaagcatg
     1381 tgttgatcgt ggcagatgaa catagtgaat tttcttatct ggaacgtttc cagacaacca
     1441 aagaacaagt agcaaaatca tcaggtaata tcatcgtgga agtcatcgct aaagctggtt
     1501 caaaaatcaa atattcagcc gtagatcaat taggagaaaa tattacttct tatatgaatc
     1561 gccgtgggca cattttacga gatgcttctg tcgactgggc aattggtgtc atgaatgacg
     1621 gtcatgtgat cgctgacttt gactcagatt tagcaggaga aggtgcacac gcagaagtca
     1681 aaatcgtggc aatcagctcg ggcagacaaa tccaaggaat cgatacacga gtaaccaata
     1741 aagcccctca tacgatcggg catattttgc aacacggagt gatccgagaa aaaggaacat
     1801 tgacctttaa tggtatcggc cacatcttga aaggggcaaa aggtgcagat gcccaacaag
     1861 aaagtcgtgt cttgatgctt tctgataaag cacgtggtga tgcgaatcct attcttttga
     1921 tcgatgaaaa cgaagtaact gctggacatg ctgccagtgt aggacgagtt gatccagaag
     1981 aaatgtacta tctgatgagt agaggattgc ataaagaaga agcagagcgc ttagtgattc
     2041 gaggattcct cggttctgtc ttaacagcaa tccctgtaga acaagttcgg aaagaattag
     2101 tcgaagtaat cgaagggaag ttgaatggat gatcgatgct aaaaagctac gcaatgattt
     2161 tcctatctta tttcaagacg tcaatgatga acctttagtt tatctggata atgcagcgac
     2221 gacccaaaag ccaaaacaag tactccaggc attagcgtac tattacgaac acgataacgc
     2281 caatgttcat cgcggtgtcc atacgttagc agaacgcgcg acccatgatt atgaacaagc
     2341 aagagaaaaa gtacgggcgt tcatccatgc aaaagaaaca gcagaagtgc tgtttactcg
     2401 tggtacgacg acaagcttga attgggtagc cagaaccttc ggcgaccgct atatcaaagc
     2461 aggagacgag atagtgctct cttacatgga acatcattct aatgttatcc cgtggcagca
     2521 gttggcaaaa cgaacaggtg ctgtcttgaa atatatcgaa ctaacagaag aaggcttcct
     2581 ggatatggag gatgcagcga aaaagattac ggacaaaacc aaaatcgtct ccattgctca
     2641 tgtatccaat gtcttaggtg tcttcaatcc tgttcaagaa ttagctgaac ttgtccatga
     2701 aaacggcggg gtcatcgtag tagatggtgc tcaatctgcc ccgcacatgg ccattgatgt
     2761 ccaagcacta gactgtgatt tctttgcttt cagcggacac aaaatgtgcg gaccaacagg
     2821 tattggtgtt ttatatggta aacgaaaatg gttagaagaa atggaacctg ttgaatttgg
     2881 tggcgaaatg atcgattttg tcgaattata cgacagtaca tggaaagaac taccttggaa
     2941 attcgaagca gggacaccaa atatcgccgg cgctatcgca ttaggccatg caatcgatta
     3001 tttgcaaaaa ctcggtatgg acaatatcca tcgatatgaa gaagaactag ccgcttatgt
     3061 gttgccaaaa ttacaagcaa ttgatggatt gactgtttat ggtccacaag acccgaattt
     3121 tcggacagga gtcgttgcat tcaatttgga tgggttacat ccccatgatg tcgcgacagc
     3181 tttagacatg gaaggggtag ccgtcagagc aggtcatcat tgtgcgcaac cgctattgaa
     3241 gtaccttgaa gtatcagcga ctgctcgcgc gagcttttat ttctacaaca caacgcaaga
     3301 cgccgaccgt ttagttgaag caatcatggc aacaaaggag tttttccaac atgggactat
     3361 ctaaattaga caatttatac cgtcaagtga tcttggatca ttcttcccat ccgcatcatc
     3421 gtggaagctt gactgactcg acaagcaaga tcgaattgaa caaccccaca tgtggagatg
     3481 tgatcgaatt gcaagtcgat gtaaaagacg gaatcatcaa aaatatcgcc tttgacggca
     3541 gtggctgctc catcagtacg gcaagtgcct cgatgatgac agacgccgtc atcggaaaat
     3601 caatagaaga agcagaacat ttaactgtag actttctaca gcttgtccaa ggaaaagaag
     3661 tcaaagatcc cgaccaatta ggcgatgctg ctatgcttgg tggtgtagct aaatttcccg
     3721 ctcggatcaa atgtgcgaca cttgcctgga aagcactagg aaaagcaatg gaaaacgatg
     3781 gtaccgcagc tttccatcat taccacaagg aagaggagtg agaacatggg cgtaccagaa
     3841 ttagaagaat ataaattcgg gtttcacgat gatgtcgaac cggttttcag tacaggagaa
     3901 ggcttgacag aagaagtgat ccgtgagatg tcaagaatca aaggcgaacc agaatggatg
     3961 cttgaattcc gtctgaaatc tctagaaaca tttaacaaaa tgccaatgca aacatggggc
     4021 cccgaccttt cagacatcga tttcgatgcc atcaaatatt accaaaaacc aagtgacaaa
     4081 cccgctcgtg actgggaaga cgtaccagaa aaaatcaaag atacatttga aaagattggt
     4141 ataccagaag ctgaacgagc ttatctagcc ggtgcttccg cacaatatga atcagaagtc
     4201 gtgtaccata atatgaaaga tgaattcgaa aagttaggaa tcgtctttac tgacactgac
     4261 tctgctttga aagaataccc tgaattattc aaagagtact tttcaaaact tgtaccgcca
     4321 accgacaaca aattagcagc gctgaattca gccgtatggt caggtgggac ctttatttac
     4381 gtgccaaaag gcgtcaaagt cgatgttcca ttacaaacgt acttccggat caatgcggaa
     4441 aacacggggc aattcgaacg tacgctgatc attgttgacg aaggagcaag catccactat
     4501 gtcgaaggtt gtacggcacc tacttattca agtaacagcc tgcatgccgc catcgtagaa
     4561 atctttacaa gaaaagatgc gtattgccgt tacacaacga tccaaaactg gtcagacaat
     4621 gtgtacaact tagtaaccaa acgagcaaaa gcatacgaag gcgcaaccgt tgaatggatc
     4681 gacggaaacc taggtgcaaa aacaacgatg aaatacccaa gtgtttactt agatggaaaa
     4741 ggtgcacgag gcaccatgct ctccatcgcc tttgcaggag caaaccaaat ccaagatacg
     4801 ggtgcaaaaa tgatccacaa tgcaccaaat acatctagct ctatcgtctc taaatcaatt
     4861 tcaaaagacg gcggagaagt aaactatcga ggacaagtaa cctttgccaa aaacagtgcg
     4921 ggttcgatct ctcatatcga atgtgacacg atcatcatgg acgaatggtc gaaatccgat
     4981 acgatcccat tcaatgagat ccacaacagc caagtctctt tagaacacga agccaaagtc
     5041 tcaaaaatct cagaagaaca actctactat ctgatgagcc gaggcctgac cgaatctgaa
     5101 gcaactgaaa tgatcgtcat gggctttgtt gaaccattca ccaaagaact tccaatggaa
     5161 tatgcagtcg aactcaatcg attgatcagt tatgagatgg aagggtctgt aggttaataa
     5221 ctatttcatt gctcgaaacg ttgatattaa agcgtttcga gcgttttttt atttgtattt
     5281 taatggttgg ctacgttttt ggctacgttt ggaattattc gcccataaat tttgcaaatc
     5341 ggtcaccagt tttcttaaca gattgtggtg tgacatgagc atagatattc atggtagttt
     5401 taatatcttt atgacctaat cgttcctgta cttcttttat cgacgcacca ctttcaaaca
     5461 ataagctaca gtgggtatgc ctaaatccat gtggagtaat ttgaggctcc atcggtgtct
     5521 ttttgtaaat ccagtctaac caatcgttta ctacttgggg gtagtacaac ttgtttgtgt
     5581 ttgtagtgaa aagaaactgt tttggtgaat tcgtgttata gccatataag atcattattt
     5641 tcatttgttc ttgttgccaa atacgcagtt gtttaagtgt aaaatcatca agctgaatcg
     5701 ttcttgatga atttttagtt ttaggtactt gtagaactac ttgattaaac tcgtcttgag
     5761 cgatagtttt accaattgtt attgttttat tgaaatagtc tatatcttcc cattgaagcg
     5821 ccaatgcttc gcttttacgc attcctgtat aagctaataa acggaaaaaa gtgtacagtt
     5881 taactgaatt attttcttta gtattctgta aaaacagttg taattgatct ttcgtgtaaa
     5941 acttcaaggg ttgttcttct tcaatttttc ttggaagcaa tgttttagac ataggattat
     6001 ccttacagac ttccattgct actccaaatt taaatatttg agcagttgtt tttctcaaga
     6061 aaccatattg ttttaatgtt ttgtgccatt cattcacctg tttttggcaa tagctaacac
     6121 ttattttatc caatcgtaat tccccaaaag caggtaatat atggtttttg caaaaacgtt
     6181 gagcaacggc aacagaagac ggttttactc ttgttctgta gttatcaatc cataattcat
     6241 acaactcttg aaaagttgta acattgattt taaactcttg tttttcttta aagtctacaa
     6301 gtagtctatt aagtgctgtt tgcgcctcag cttttgattt gaatcttcta cgctttgtac
     6361 gtatatcttt tccagtcttg ggatctttcc ctaaataagt ttcaaatccc caatattttt
     6421 cgccattttg taatttataa ctaaaaaatg ttgccatttg attctctcct tacttcatta
     6481 ttgcaggtag agaagttatt tgaagaatca attattttta aatttatcgt gattatcagt
     6541 taaaaatttt gcgattatac tatttttctc atatgggaaa ttgtattttt tactaagatc
     6601 tgaaataaat ttttctgttt caaattggtt tttagtaatt ttataaaaga aatcctcgac
     6661 agatgaatac tttgaaatat ctttataggc taagtccatt tcgtctaata gctgttttaa
     6721 agaatcaatc attctttctt tttcaccgta tttgaaatca ccatttctta tcttgtcttg
     6781 aagtttactg ttggctcttt tgaaaaatag ctct
//
LOCUS       DACCXW010000131         6771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCXW010000131 DACCXW010000000
VERSION     DACCXW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.137"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            4..894
                     /locus_tag="IVY71_002449"
     CDS             4..894
                     /locus_tag="IVY71_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7986477.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(967..1791)
                     /gene="nadE"
                     /locus_tag="IVY71_002450"
     CDS             complement(967..1791)
                     /gene="nadE"
                     /locus_tag="IVY71_002450"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7986478.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1803..3275)
                     /locus_tag="IVY71_002451"
     CDS             complement(1803..3275)
                     /locus_tag="IVY71_002451"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7986479.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3912..4274)
                     /locus_tag="IVY71_002452"
     CDS             complement(3912..4274)
                     /locus_tag="IVY71_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7986480.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4497..5249
                     /locus_tag="IVY71_002453"
     CDS             4497..5249
                     /locus_tag="IVY71_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7986481.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5389..6312)
                     /locus_tag="IVY71_002454"
     CDS             complement(5389..6312)
                     /locus_tag="IVY71_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7986482.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6327..6599)
                     /locus_tag="IVY71_002455"
     CDS             complement(6327..6599)
                     /locus_tag="IVY71_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986483.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2066 a         1350 c         1157 g         2198 t
ORIGIN      
        1 tatatgcaac tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa
       61 acaaggatca agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca
      121 aaagaacttg gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca
      181 aacatcatca aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa
      241 atcgatatca gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata
      301 cctattggtc actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat
      361 gattttatcg ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc
      421 cgcattctct ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca
      481 gaggatacat tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca
      541 aatgacaatt ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg
      601 acgagccctg gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat
      661 aatcttaatc aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg
      721 aatggagaac ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg
      781 catttgcgaa tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt
      841 aaaggattcg gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt
      901 tcttttctat tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct
      961 ttattgttat ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt
     1021 cttgttccac catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag
     1081 ataatcatca atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt
     1141 cccatcttca agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc
     1201 ttgaagcaat tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc
     1261 accatcacca aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa
     1321 tacggctcct gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt
     1381 gatatttcct ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg
     1441 tgcatcaaca gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag
     1501 agcagctttt gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa
     1561 ttgatagttt tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg
     1621 gcctgctaaa gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg
     1681 atattttttc aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc
     1741 aattgttggt ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc
     1801 tcctatgctt ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt
     1861 ttatggttcc agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt
     1921 ttatactctt cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt
     1981 gcaggtaatt cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct
     2041 gtaaagtctc gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct
     2101 tcttcttctc gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca
     2161 aaatttcgag tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg
     2221 cttgataact tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg
     2281 aatactgcac caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat
     2341 acatcaattt tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc
     2401 gaagcataga tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta
     2461 gatatatagg ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct
     2521 aattctttcg ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg
     2581 actaagaaca cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt
     2641 ccataagatt ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc
     2701 ccagcacgta cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag
     2761 actgctgcat ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg
     2821 atgacagatt tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc
     2881 aacagtgctg tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt
     2941 tcatttgcaa aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa
     3001 ttttgtaaat actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata
     3061 tcggaatcat tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa
     3121 accgcgtatc cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa
     3181 tcagctcgac ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc
     3241 agcgtcaaac tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt
     3301 ttcatttcct acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc
     3361 tgaaaaagca catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact
     3421 atcctttctt attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc
     3481 cgagacacga agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc
     3541 ctactacaat cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta
     3601 caacataatc aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt
     3661 atccccaaag aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat
     3721 gacaagatat accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag
     3781 atacaaagta agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca
     3841 gtctcgcctt gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt
     3901 attagtcgtt attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta
     3961 tttggacgtt tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact
     4021 ttgacatctt ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt
     4081 gtgatttttt tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat
     4141 agcagttgtg ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa
     4201 aagaattcca ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt
     4261 tcgattagct tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt
     4321 atgttaccat ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag
     4381 gatttacctt ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt
     4441 tcttgaccat ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg
     4501 agtacacaat aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt
     4561 atgatgagat tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg
     4621 gcaaaaaaga agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt
     4681 tggatgatat ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca
     4741 agcatcagca aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc
     4801 aacagacact tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag
     4861 ggtttaaaca acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa
     4921 aatacggaaa aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa
     4981 cgtatgaaga aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa
     5041 aagaaccaga agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct
     5101 cttacagctg gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag
     5161 ccgatgaacg gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt
     5221 taaatgaaat tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg
     5281 taaaatgacc taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg
     5341 tgtctgctcc attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat
     5401 aaacgtataa ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat
     5461 ttctccgcaa gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag
     5521 caaattatcg cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc
     5581 tgctgcaacc atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa
     5641 gcctgcttgt tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa
     5701 agatggaaaa agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc
     5761 acttcgcccc aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc
     5821 ttctgtaatt gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc
     5881 ttcacaaaaa actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc
     5941 acgagacgta atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg
     6001 ctctttttct ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc
     6061 tgcttcaatg atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat
     6121 cactccaatg cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata
     6181 atcgacttct ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg
     6241 tccattgatt acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg
     6301 aatgtttgcc actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc
     6361 acagctggac cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc
     6421 tgcccgcccc aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt
     6481 actatattcc tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg
     6541 acaaacgcat aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt
     6601 gcaagccctc cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt
     6661 ttgttttgcc aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa
     6721 attgaataca ttataatgat actgtaaata tatctttatt attagtgaat g
//
LOCUS       DACCXW010000132         6746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCXW010000132 DACCXW010000000
VERSION     DACCXW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.138"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            17..2344
                     /locus_tag="IVY71_002456"
     CDS             17..2344
                     /locus_tag="IVY71_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7986484.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2470..2799
                     /locus_tag="IVY71_002457"
     CDS             2470..2799
                     /locus_tag="IVY71_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7986485.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2839..3843
                     /locus_tag="IVY71_002458"
     CDS             2839..3843
                     /locus_tag="IVY71_002458"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7986486.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3840..4286
                     /locus_tag="IVY71_002459"
     CDS             3840..4286
                     /locus_tag="IVY71_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7986487.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4436..5251
                     /locus_tag="IVY71_002460"
     CDS             4436..5251
                     /locus_tag="IVY71_002460"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7986488.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5457..6629
                     /locus_tag="IVY71_002461"
     CDS             5457..6629
                     /locus_tag="IVY71_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7986489.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         2393 a         1269 c         1338 g         1746 t
ORIGIN      
        1 tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc aacgtatatg
       61 tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact tacatcccat
      121 tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta tttatgtaaa
      181 cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa atagtccacc
      241 ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg agacaacctc
      301 aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc
      361 agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta ctgttgcggc
      421 cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat atgccaaaga
      481 tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt tacaaggcgt
      541 aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt ttattggctc
      601 cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg tggctaccgc
      661 tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt ttacaccctc
      721 aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg gtatacagtt
      781 agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg ttgatggtaa
      841 gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca atgcgaatcg
      901 gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa cgtacgggac
      961 tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag cagtaacggg
     1021 ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg tcgatacggt
     1081 gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt ttggacagat
     1141 gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag acctgacgac
     1201 taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgatag aaacaaagaa
     1261 ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa tcgtaaagga
     1321 cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg caacagtatc
     1381 aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga tcgttaaata
     1441 tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca gtattgatca
     1501 aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg aagtaactgc
     1561 gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac tagctaaact
     1621 agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct atacgattaa
     1681 gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa aagcaggtgt
     1741 cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc cagtacaagt
     1801 ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata tggttaatgc
     1861 tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag cagcaaatcc
     1921 caagttgtca tttactgata tagccaacac atcaggaact tataccatct ataaagctaa
     1981 cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc actcgatttc
     2041 ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt ataccctaac
     2101 agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag gcgaaggaag
     2161 tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga tcaatccgac
     2221 tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt taggactgat
     2281 ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag gtgaacagcg
     2341 atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt tatcctactg
     2401 gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac atttactttg
     2461 cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga cggaataaat
     2521 atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc tatatatgat
     2581 gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga
     2641 aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac aaaaacagta
     2701 gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca aggtcgtgac
     2761 gctgtttacc gatttacgga aataaagact tcagattaag taaaagaaaa atcagcacca
     2821 tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac catccacttg
     2881 tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac agtagagaat
     2941 aagcagacag attttgcaga tggggataaa gtcccttatc tcatcaccgt aacaattcct
     3001 gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc tcagttatgg
     3061 ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac atttcacaca
     3121 caaaaaataa aacatggttt tgtcttatca gattctggaa aagatcttag ttcgtttgct
     3181 ggaaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga aaaaacgaca
     3241 tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca tcttgccatc
     3301 caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa actagcagat
     3361 gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg gataaatcat
     3421 tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga tggaacattt
     3481 tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc gcctaaagga
     3541 tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta tgatccgctt
     3601 catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa taatggaaaa
     3661 acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg aagctttcca
     3721 aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttattgct agtcctttca
     3781 acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag ggaaataaaa
     3841 tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg ctgcttcctt
     3901 tcgttctcgg cctaggaact gcagaggttt ctgcagccgt atcacctaca ccagaaaatg
     3961 ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa ataaacaacg
     4021 gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc aacgtatatg
     4081 acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac gctacaccct
     4141 tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca ggtaaatctg
     4201 atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc gaaacgtcgc
     4261 caaatatcgt gttaagctta ccctagaccc tgacaaacct ttagacacga ttcacctata
     4321 tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga acttttcagg
     4381 ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc aattcgtgac
     4441 cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag cgttgacatt
     4501 cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca attatacttt
     4561 agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag aaactacttt
     4621 tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg cagatgaagc
     4681 caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg ataacacgat
     4741 tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag atgtgaacac
     4801 gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc caactgttaa
     4861 attaatgaaa aaaatagcaa accttgcctc aggagaatac ccatatgctt ggtcagatga
     4921 agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag ggaaattttc
     4981 aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac cagaaggcta
     5041 cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg atccaacaac
     5101 ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa ctggtggtat
     5161 ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag tgatctattt
     5221 caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc atacgataaa
     5281 taaacgatag tgattcaaat ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt
     5341 cgctcaacaa acggcaaaat aggaggaact attttgccgt ttgcttcgct aatactcagg
     5401 tcaaggcgct gatttcacaa cctcttcata tacatattag aaaataggtg gacatgatga
     5461 ataaaagcag aataagaata ctggacatct tcatggcaat cattttagtc gttgggatcg
     5521 ggatcttttc ttatcctttt gtagaagatt cactgaatga ttttcttgcc cagcaaatga
     5581 tcatccatta tcaaaagcaa gcatcgaata agaattcaga ggaaataaaa aaacagcagg
     5641 aaaaaatgac aaagaaaaac caacagttgg cagagcaaaa cgtatcacca gggataggca
     5701 gtttcaacca agcagttgat gcaaaagcat taaaagattt acctagtaat gcctttttta
     5761 tggaacatat gcttggagtc atcgaaatcc caaaaatcaa tgtcagctta cctatattcg
     5821 atcagacaac ggaaatattt ttgcaaaaag gaacgtcatt attggaagaa agttcttatc
     5881 cgactggcgg gaaaaacacc catgccgttc tttctgggca tcgtggactc ccagaagcaa
     5941 aattatttac agatttgcct aagttgaaaa aaggagacca gtttttcatc cagatcaatg
     6001 gaaagacatt ggcgtatcaa gtagaaaaaa tccaagtggt cttacctgat gaagtagatt
     6061 ctttgggtat ccaaaaaggc agagatctcg tgactttgct tacctgtact ccttatatgg
     6121 tcaatacaca tcgactgctt gtgacggggc atcgtattcc ttatcaagca aaagaggcaa
     6181 aaaaagcgat ccaaggaatt gatcaatgga aaaaatggaa attttttatt tggtttatcg
     6241 gtatactact tggatcaatc ggcttagttt ggctactcat cgcttattta gattctttgg
     6301 ccatcgctaa gcgaaactat cctttatctt tttatgtaaa gaacacaaac ggacgaccaa
     6361 tcgaaggaat ggtcttttct gtaaaaacgc tcaacggcaa acactatatt actagagaaa
     6421 aagttccatt tgtgaaagct tctgatgaat acggactagt gagattttct gatttaaaag
     6481 gcaggaacta tcgcttgcaa catgaggagc tcctgcttaa aatccatgta aaacataaac
     6541 attcgaaaca gttttcaatg aaattgaaaa aaggaagata taaactgcga aaagaaaaag
     6601 aggtgtacta tttgattgag aaagaatgaa tgaagatgtc caaaaaagca gagggaacag
     6661 actaaatgaa aaaagagaga agctgacgaa atggacgtgt aaactgttat ttttggttta
     6721 ctatctggaa cgccatcttc tatttt
//
LOCUS       DACCXW010000133         6704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCXW010000133 DACCXW010000000
VERSION     DACCXW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.139"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..579
                     /locus_tag="IVY71_002462"
     CDS             <1..579
                     /locus_tag="IVY71_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7986490.1"
                     /translation="CNRTYYFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLK
                     HLGEYEVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEI
                     GIRRALGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTI
                     LLAVGVSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            980..1585
                     /locus_tag="IVY71_002463"
     CDS             980..1585
                     /locus_tag="IVY71_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7986491.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1601..2173
                     /locus_tag="IVY71_002464"
     CDS             1601..2173
                     /locus_tag="IVY71_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7986492.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2425..2682)
                     /locus_tag="IVY71_002465"
     CDS             complement(2425..2682)
                     /locus_tag="IVY71_002465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986493.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2808..3623)
                     /locus_tag="IVY71_002466"
     CDS             complement(2808..3623)
                     /locus_tag="IVY71_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986494.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4140..5465
                     /locus_tag="IVY71_002467"
     CDS             4140..5465
                     /locus_tag="IVY71_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7986495.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5458..5808
                     /locus_tag="IVY71_002468"
     CDS             5458..5808
                     /locus_tag="IVY71_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986496.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5790..5975
                     /locus_tag="IVY71_002469"
     CDS             5790..5975
                     /locus_tag="IVY71_002469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986497.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT         2297 a         1028 c         1256 g         2123 t
ORIGIN      
        1 tgcaacagaa cctattattt taagtctgaa aaggatacat cgtctctaac gttaacttta
       61 gaggaaggag ttgaacctga caaggtgacc tctaaagtga taaaccaatt gaaagaaaaa
      121 ggtagtttga aacatttggg ggaatatgaa gtgttagata cggctatgtt aacgaaagga
      181 attggtcaaa tcctcagtac gatcacttat tttataacag ctgtagcagg tatatcttta
      241 tttatcgcag gggtaggtgt aatgaacatg atgtatatat ctgtatctga acgaacaaaa
      301 gagatcggta taagaagagc cctaggagct actcgaaaat cgattatgct gcagttcctt
      361 ttagaaggtt tgattttaac tctttcagga gggttaattg gctatttatt aggaatggct
      421 cttgcttata gtgttggttc tttgataaag gtaaacgtgt cacttgatct atttactatt
      481 ttactagcgg ttggggtatc ttcggttatt ggactggtct tttctgtaat gcccgcttca
      541 gaggctgcaa aaaaagattt aattgatatt cttagataaa atcagaatga caagaatatt
      601 tacgttctag attctaaaat aaaacacaaa gagaccaacc aggcacccta ttgtttcttt
      661 gtagtataga gatattcttt ggcttgtaag agatcaatag agttaggtga ccggagttcg
      721 aaataaagga aatttttgat tgtataggag aattctttat ccctaatatt tagaattttg
      781 agtatgccat gttgatgttt tagattctct ataaatatag aggtgtatca aaggtcaact
      841 agactgtgca aaatagagac acttgaaaaa tctatcgggg gtcagaaact taatagtttt
      901 tgacacataa acaaaatcgc gatataatgg ggttgagagt gtttcataaa cttatgtcat
      961 aaagttaagg agatttcgca tgattttaga gaataaattg ggattaacca atcaagttga
     1021 gttggctaag gttgaagaaa aactcagtaa acaaaaagcg aaagaattgt acgattgtgg
     1081 aaaaataaat gaaatcgaag ttggtacatt taaaggttta tctgaaattc atgaatttct
     1141 attttcggat atttacgatt ttgccggaaa aatccgatcg gtcaatattg caaaaggaaa
     1201 ttttcgattt gcaccagtta tgtatttaga acattcgtta cagcatattg atcagatgcc
     1261 acaaacttct tttgaagaga ttatcaagaa atatgtggag atgaatatcg ctcacccatt
     1321 tcgagaagga aatggccgaa gtactagaat ttggctggat ctcattttaa aggaagaatt
     1381 gcaaaaagtt gtagattgga acttaatcga taaagcagac tatctttctg caatggagcg
     1441 aagtcccata aacgatttgg aaatacgcta tctaatttcc aatgctctga ctgataaaat
     1501 tgacgatcgc gagttatata tgaaaggaat cgatgtcagc tatttctatg aagggtattc
     1561 tgagtatacg atagatgatt tgtaaaagta ggtggaccaa atgacgaaat atggatatgc
     1621 acgtgtctca accgcttctc aaagtacaaa agaacaaatt caacagttgc ttgagaacgg
     1681 cgtagagaag aagaatattt tttcagaaaa atttacgggt acaaaacggg atcggccaaa
     1741 attcaatcag ctgttggctg gtctgaagcc tggagatgaa atagttgtta cgaagcttga
     1801 tcgatttgct cgtaacacga gagaagcatt agagatcatt gaacctttac tcgatgataa
     1861 cgtaacgatc aaggttttga atctaggaac gattgaaaat acgtccatgg ggcgaatggt
     1921 gacacggacg ttgttgagtg tagcagaaat ggaacgtgat atgattgtgg aacgtaccca
     1981 agaaggaaaa atgtttgcaa gaaagaataa tcctaatttc agagaaggac gtccgaaagc
     2041 aacaatcaca ccgaagaaga ggcacgcata tgaactgctt acttcaggta aatcgtataa
     2101 ggaagttgaa gctataaccg gtttcagtcg cagtacgctt tttagaataa agaagaaaat
     2161 tgaagaaagt taaaaaagaa gtctcagcga accagacagg taaatactta atgcgttcta
     2221 gatatgagtc tattagcatg ttgtgaaata taatattttt ttatttactt gatttctgct
     2281 caaaaaatgt gtatggtaac tgtaaaaggt aaagtaatta aaggtatgct agccacgctt
     2341 actatattag gataccatca tatatttata atgaacgatt tgttcgtcta ccaaacaaca
     2401 catatcgttc agcgtaagtc acgtttagga acgtggcttt tttagtgtta ttatcgataa
     2461 ataatctatt gactctattg attttatctc catatcccta cggtgttgat tgtctttaga
     2521 tctttcattt aagaaccaaa taactatcat aaaaatcagc actgctgcta atataataaa
     2581 cagcattcct attgtgatat tacctattga ttttaccata actacgatta aagtaataat
     2641 ggcattcaag tctcctttgt tctctatata taaattagcc ataaatcatc ttcctttcaa
     2701 attaagatac ttgtttgata ccaataaata gtaagcgtgg ctagcatacc tttttctgta
     2761 atagcaagtg ttctaatagt ccaaacttaa tgatggaccg atgctagtta tagtacagta
     2821 atgttttctt tcttatctag atttttagta agctctatca gcaaatctaa caaatctgag
     2881 tccttttcag aaactaaatt aaatctagtg tttacaacaa agttttcatg ttctatattg
     2941 tttagattat ttgcggaaag gagggcttct agatcttgac attcaaatct atatgcatcg
     3001 tcataaatct tactagaagg agtgcttaat tcgaaattac ttattttctc atcttggacc
     3061 aagataaatt tatagatagt atctttttgt gtttttcttt taaaaacaca acagagtgtg
     3121 ttataaggaa cgttataatt ttcatcagcc tcattttttc tccattcaaa caaatgataa
     3181 actgcaacca agttgccatc aatttcatgg acagtgctct cggtaacatc ctttaaagaa
     3241 ctatcaaggg ctaatttata gtaactagag agaggaagta aaattgtcga tttactgaga
     3301 tggttatcgt ctatacgtgc aatcatacct aaatcaacta aggttttatt ccaggtgttt
     3361 atagttttag ttgtcactcc tagagactct gctatggtat tgaccgaatg ccaggaatcc
     3421 ccgccttcat tctttgcctt aaaacagtaa tacagatata aattaatagc tttgttattc
     3481 ggtaaatatt tttcaagttc tgttggtaga ataaagtaag aatccttaat gttttttctg
     3541 aactgtctcc agtctctgta gttttgttta taaagttgtg aaacggtttt tccaccttta
     3601 gaaagtcctg ctatcttaac caatcttatt gctcctttct tgaatctgtc atgatcatac
     3661 agtatttttt acttgtagtc cagcacctta atatattatc ctatgtaatt aatttcgtac
     3721 tagatagtaa ttatttttta tttaattctc tagtcactta caaataagtt atataaacta
     3781 cgattaataa attattacaa aaactattaa gaaaacttct acatacaact attacaaaac
     3841 tattaagaaa ctgaatactc ctattttttg tttttaagga aagttttttc ttttttgaat
     3901 ttcttatttt tttctaatat aactaagcaa aactggataa tacgacactt attttcaaat
     3961 tttcacaaga ctaatagtaa atatccattt aataaagtga tacaatgtgt tgattttaca
     4021 aatcaacaga agaagtaggg aaataactta cctatgcttc tttttttgta catttaatcg
     4081 attcttcttt acaaaaacga acgtatgttt gtataatgtg gaagaatgga gtgttattta
     4141 tgatggatta ttcaaaagag ccagtaaatg attacttttt gatcgatatg aaaagctttt
     4201 acgcaagtgt cgaatgtata gaaaggaatt tagatccatt aacgactgaa ttagtcgtca
     4261 tgagtcgagc ggataataca ggatcagggt tgattttagc ttcttcacca gctgcaaaga
     4321 agaagtacgg tatttctaat gtcagtcgac cacgagatct accgcaacct tttcctaaaa
     4381 cgttacatgt cgttccacca cgcatgaatc tctatattaa gcgaaatatg caggtaaaca
     4441 atattttcag aaggtatgta gcagacgaag atttattgat ttactcgatc gatgaatcga
     4501 tcttaaaagt gaccagatca atgaatcttt ttacgactga aggaacaaga agtcaacgga
     4561 gaaagaagct ggctcaaatg atccaggaga atattaaaga agagctagga ttgcttgcta
     4621 cagtaggtgt cggagataat cccttattag ctaaacttgc tttagacaat gaagcaaaac
     4681 ataatgatgg attccttgct gaatggacgt atcaaaatgt tcctgataaa gtatggaata
     4741 ttcctgaaat gacagacctt tggggtattg gatctcgaat gaaaaaaaga ctcaaccaaa
     4801 ttggtatcat gagtattaaa gacttagcta attggaatcc atacactatt aagagccatt
     4861 taggtgtgat tggcttacag ctgtattttc atgcgaacgg gatcgatcga acagatatag
     4921 cgatcccacc agaaccgaca aaggaaaaat catatggtaa ttcacaagtg ttaccacgag
     4981 attataccag gagaaatgaa atcgaactgg tggtaaaaga aatggcggaa caagtagcta
     5041 ttcgtatcag gcaacataac tgtaaaacag gttgtgtcca tttatacatt ggcacgtcga
     5101 tcttagaaac gagaccaggt ttttcacacc agatgaagat ccctataaca gataatacaa
     5161 aggaattgca gaattactgt cttttcttgt ttgataagta ttatgaagga caagaagtaa
     5221 ggcacgttgg aataacctat agcaagcttg tgtacactga ttcgcttcag ctggatttat
     5281 ttagtgatcc acaaaagcaa attgacgaag aaaatttaga taaaattatt gacaagattc
     5341 ggcaaaaata tggttttacg tcgattgtac atgcttcttc tatgttagaa ggcgctcgta
     5401 gtattactag aagtacattg gttggtggcc atgcaggcgg cgcaggaggg cttaaaaatg
     5461 attgattata atgatcgtgg acgtttaaag tgggcaggtt tttatttatc tgatcatact
     5521 gaaaaaattg atgccgacaa tgaacaacga gccaatcaaa atttggcaaa agaacaaatg
     5581 acgactgaag aggttgcgga agtattgaac gctgcagtca taaaaaatag atcagtatgt
     5641 attcaaaaag aagaacgaaa cgctgaagga tactatccag aagatattgt tagcaagatc
     5701 ttaggtagtg atgagctggg gatctacgtt ggaaaaaaca aagttcacta cgacgaaata
     5761 cgtcatatcg aattttacca ggataataaa tggggtcgat tagaatgaac attatccagc
     5821 aatatgagct aaagtatata acctttgatc agctatcaga agagatttgg ggttatggcc
     5881 agcgtttgat aaatgaggtt ggtgttgaac gcttctcttt ctatgtagaa gctgcagcag
     5941 gataccataa atacgaatac tatattggct attaaagtgc aataaaatta tatttctttt
     6001 atgttttaca ttttgtatca attctgataa aattacctat aggtaattaa aaaatataaa
     6061 aaaactagtt acaaccttag gacgttgtaa ctagcttgaa gcatactaga gctaactaaa
     6121 atagcactag aaagcaatgt taaacccgtc caacaaactt gatttaacat tgagaccagc
     6181 ttattcagct aaatatagtt gaataagctt cttttgtttt aaaaaaacaa aaggtgtact
     6241 acgaataacg caagtaaaag aaatactatt acaaccagta ggctacaagt aagtattact
     6301 aaacattgcg tagttagaac ctttgctcta ttgtctaaag ccacaaaatt agagatcagc
     6361 tttatgatgc taagagcgag ctgcatccat gcggacatag gttcttttga cactattaat
     6421 tctccttttt aggataaggg aaattgctag tagttttttc attagcacca tcccccttca
     6481 tttgattgag tctagtgctg ctgtgatagt ttgctgttta atatgcttac attcttatta
     6541 taacataaat gaaacaaaca catggttaga ggtcgtatca atcaaaatag tgtctcacaa
     6601 acgatttatt acttactcct gctgactaac ggactgttct tcagtaactg taactatatg
     6661 tttgttaaaa ccaccattac ctgtagataa aaaccaatat attt
//
LOCUS       DACCXW010000134         6691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCXW010000134 DACCXW010000000
VERSION     DACCXW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.140"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            207..2882
                     /locus_tag="IVY71_002470"
     CDS             207..2882
                     /locus_tag="IVY71_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP7986498.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3064..3303
                     /locus_tag="IVY71_002471"
     CDS             3064..3303
                     /locus_tag="IVY71_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986499.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3319..4692
                     /locus_tag="IVY71_002472"
     CDS             3319..4692
                     /locus_tag="IVY71_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7986500.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4696..6330
                     /locus_tag="IVY71_002473"
     CDS             4696..6330
                     /locus_tag="IVY71_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7986501.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6493..>6691
                     /locus_tag="IVY71_002474"
     CDS             6493..>6691
                     /locus_tag="IVY71_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986502.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2350 a         1075 c         1339 g         1927 t
ORIGIN      
        1 attgaacaat attttcctaa ataatttaat aaggattgat ttatcgtaaa agaaaaaagt
       61 tgtgatttcc aaaatcacaa cttttttctt ttgtcaagat tttgagtggg atatattttt
      121 gtagaactga agatcatgaa attatgagag gttggaggta atgtagtcat acaaataaaa
      181 attttttaga aagtgagtgg taattaatga aaatgaatca cacttcaatt cagaagcatt
      241 tacaactgtt aaatacactg gggatttctt atgttaattt gagaactttt tctacattaa
      301 acaagaaaac cgtttctcat attttttcta ctgcagattc tcaagcaatt attaactatt
      361 tagaaaaaca gaaattgtct gaaggtacag taaacataac ttataatcct attaaacaag
      421 aggtgattac acaaaaaaag cattctgtga gagacaatga tattgaaaaa attcggtttg
      481 tatttattga tattgaccct aaaaggaaag agggaagtgc aacagatagc gaaaagaaaa
      541 aggcagaaaa tgttatggaa caagtagaac gttatttgaa agaaaagaga atagaatcat
      601 ttgtaaaagt tgatagtggg aatggctacc atatttttct tcccataaat gaacaaccaa
      661 ataatcacga gacgattctt actattcaga attttcttca attacttcac cgaagatttg
      721 gagtagaaga tgaagttgat attgatactt ctgtttataa tccttcaaga ttatgcaaat
      781 tagttggtac accagcggtc aaaggaaagt ccacaaaaga gcgtccgcat cgtttatcta
      841 gtttcctttc tattcccgat aacatagata gaagtaattg ttttaaaatt cttgaacaga
      901 tagttgaaga aaattccatg atgtcaacct ctagagtaaa aagccatcca tcttttagag
      961 aagtgttacc ttttattcaa gcagactata aacaatggtt agctcactat cctgaattaa
     1021 cttataccat aaaagagggc gatcataaag gagttacctt acttatattt gacgaatgtc
     1081 cattaagaaa acacacgaat aattctaatg gttcttgttt gtctgttacg gctgataata
     1141 agattcgatt tcattgtctg catgcttcgg ataaaaataa agatattcaa gactttgtaa
     1201 aaaaatatcc gttgccaaaa cctgtaaatc cttctgtaga atcattagaa tccaatcaag
     1261 aatattattt tgaccagttt aaactgaatc aagaaggagt atttttggtg aaagaaggtc
     1321 aagaagaaaa agttgcttgt ccgatgttta ttaagcaaat acgcaaaaat atcgaacaaa
     1381 aagtcattac acttgttctt gcctattatg tcgatcattg ttgggatgaa atagaaatag
     1441 aaggtggatg tttacaaact aataatttta aacaattagc aaaacatggc ttaatcatta
     1501 aagctagaag agaagctgaa gtcgttgact ttttacagat tcaacgggag aatgctccaa
     1561 tcttttttga acatgaatct cttggctgga aaaaagaaaa tgaagcaatg atttttcagt
     1621 tacaagattc ttttggacgt gaaattcctt ctgtattaac taaaaactca tgctaccaat
     1681 taacaactaa tggtacagaa gcggaatttg acgcattggt tcataagtat gttttaggaa
     1741 cgaacttaga acttgcttgg tcgattggta ttgcttctat tgtgttaggc tatttaaacg
     1801 ttacgaaggc taatactttt ttgagtttac taattaacct acagggcaag agcacaacgg
     1861 gtaaaaccac tacgttacac tttattgcct ctttatttgg aaatccaact acggttctta
     1921 gaaatatgaa tgctaccaat aaagccatta taaagctagc atcaaacaat cagggaggtg
     1981 ttccactaat actagatgag ttaggagcag caactcaaaa taactttagc tcgttgatct
     2041 atcaattagc ttcaggagaa gaaaggctaa gattagattc taattgtcag cttatgccac
     2101 aagagacttt ttctgttttg acccttacct cttctgaaga gcgtttggaa aattatttca
     2161 atgagaaatt aactggtttg aaagttcgat atatcgaatt ttctgatatc aagtttacaa
     2221 aggatgcgaa tcacgcagag aaagttaaaa gtctttcttc taatactttt ggttggggta
     2281 taaagttatt tcttttgaaa ctctttgaac aagggattac aaaaattgaa gcaacttttg
     2341 tcgaagctca aaaaaaacta tgggctgttt tacctgaaca ttctttgaag caacgcattg
     2401 gtaataatct agctttgatt tatagtggag ccctcctaat tcaagatata ctgggctatc
     2461 ctttacgatt agagcatata cagacggtgc ttattaattc gtacgaacag ttaattgtag
     2521 atactagttt aggaagtgag caaacggtat ttgaccgagt gaaaacattg ttggtgatga
     2581 atagccataa atttttaaca aacagtgaca acaaacatac gcccagtacg atttggggaa
     2641 aagtatcaat tcaaaaaaat ggggatatta aggtaaatat ttttcctatc ccgtttgagg
     2701 acttgttaag gcgagaattt caagtaaagg atctaaccta tctttttagg caattgcttg
     2761 attcgggtaa tatgtgtgca gaaaaaggga gaagaacgaa acgtattcac atgaatcgtc
     2821 aagttcttcc aacatacgag attctattac cttcttcact caaatcgtat tttcgattgt
     2881 aatttagaat aaaccaaagt atgaaagtga ggcaccatgt tatggaaata tttcttattg
     2941 atttagcaaa acaactgatt acttatctgt atgatagcga gcaaacccaa acggaagtaa
     3001 atgaagcttg atctatgctt catttacttc attcaatcaa acatgaaaga aaggaaaatt
     3061 ctaatgacta aaaataaaat gacactaaaa gcagaggttc ttctgtatat tcaagaacat
     3121 ttttcaaatc aagccttttt tacaaaacca atttacttgg atttcgaaat aagaggggta
     3181 tctgctggat ctatcggggg tactcttcaa gcgttgaaga acgaaggata tttagaaaat
     3241 cactttgtgc aacgttcgtt caatagaaga gtcgtgaaaa aatggtattt agttcattca
     3301 tagagagggg aatcaagcat gcgtgtggct tgttatgatc gaacgtctgt ttatgaagaa
     3361 gcgaaaatta gtaatgacgc tcaatatgat gcagtgaaag agtatgtttt agagaaatat
     3421 gaacaaattc cccaacgatt ttcagacaaa ggagaatcag gggaaaaagc ggagcgtcca
     3481 gcttttcgac agcttagagc ttgggtttgt gcgtcagaag aagagggggt gatctatgtt
     3541 tggcgttatg atcgattggc acgtcaagcc aaaatagcct tagaatttat tgagttgtgc
     3601 caacaacatc aggttcaagt gtattcgatt tctgaacctc ttcctgcttc tttctcacac
     3661 gcgtcaacga ataaactatt tgtgcaatta ttgttcactt ttgcagaaca acaacgaaac
     3721 gtcattattg agaatgttcg tgccggactt tcctataaaa agcaacaacg gcaatatttg
     3781 tcttctgcgg taccatatgg gtatcgttta gtgaatggga aaatccaaca agaagtacac
     3841 gaagcaagga ttgttcgcta tatttttcaa ctttatttga ccaaaaaata tggctacaaa
     3901 aagctttgtc aacgattgac gcaacaaaag cttttcttcc gcgaacgtcc ttttcagccc
     3961 tatcatattt atagtatcct aaaaaatcca ttgtactatg gcgaaatcaa aggaggatct
     4021 ttggggaagt atctaggaac ctttgaaccg attctctcga aagcaacctt tttacaagcc
     4081 caagagattc gccaatcaag gtgtacagct aaaaaagata cgtatctgta cttattacgt
     4141 caaaaaattc ggtgtccctt ctgtggtcgt catctgagtt cgaagtatca atggaataca
     4201 aagaagacga agacgcttca ttattatcat tgtaccgacc gaagctgcca aggaattttt
     4261 cttcgggcag aaagggttga gtcagaagta ttggaaaaga tacaggcttt ttttcaatac
     4321 gaaggcgtct atcaagcctt gattgcccaa ttaacgcaac aattagccga aaagcgaaaa
     4381 gatgaaaaac gcaaagtaat ccaacaaaag aaacaagaag cacaatggat taaggcattt
     4441 gaagagggca attgttcact ggaagaactc aagcaacaac tgcaagctct tcaaaagccg
     4501 atgtctactt cttcgtttct ttcactccaa cagtgtgaag caaaagtgaa agaatggctt
     4561 caactaaaag acctctctag tcgacaatta ttatgtgact atgtttgtcg gattgaggta
     4621 acaaaggata aaacagtttg tgggatttac ttccgacaac tgacacaaaa tattttagaa
     4681 aggaacgaat gatctatgga aatgatacaa accaaacgag tcgcattgta tattcgcgtg
     4741 agtacacaag aacaagcttt agaaggacat agtattcaag cacaagaaca aaatggacga
     4801 cagtttgccg aacggatggg ctatgaagtg gtagaagttt atgccgatga aggaatcagt
     4861 gggaaatcca caaaaaatcg gttcgcctat caacgaatga tgaacgatgc tcggaaccat
     4921 cgttttgatc tactagtgat ttggaaattg acccgtctcg gacgcaatat gttagatgtc
     4981 ttacaaacag tcgaggaatt attgcgatat gacgtaggat tacattcgat ctcggaacaa
     5041 tttgacgtga ccacttcgac aggtaaattg atgttacaac tccttgggag tttcggtgaa
     5101 tttgaacgca accaaattgc ggagaatgtt caaatgtcaa tgcaaagctt agtgaaagat
     5161 aaaaagcgtt atgcaggagg ccgacgttta ggctatgtca gtggtattga cacgaatggt
     5221 caaaaacaat tggtcattga accagaagag gcgaaaattg ttcgtctgat ctatgcaaaa
     5281 tatttaggcg gagcagggta tcgagcgatt gccaattttt tgaatcgtca aggatatcaa
     5341 acagtcaaaa agaatgcttt ttctaccacg gcagtcaaag atatcttacg caataaaatt
     5401 tatgcagggt atttagaata tgcgcgctac atcaattggg aaaccaaacg acggaagggg
     5461 aagaatccga gaccgatttt agtgaaagga acccatgaac cgattatttc ggaagaagat
     5521 tatcaaaaag tacaagaacg ccttgcttta gaaggaaaac aacccaaatg gaaccataca
     5581 ggtgaaaatg tattgacagg cttgttacgt tgtccagaat gtggcgcacc gatggcagcg
     5641 agtaatgtca caaataccct aaaggatggc acaaagaaac ggattcgata ttattcttgt
     5701 tcagtctttc ggaataaagg agcaagtgtg tgtcatgcca attcgattcg tgccgaacaa
     5761 gcggaaacat ttgtcgccga ccgattaaag gagatcgttc agctaccaga agttcttccc
     5821 cgattggtgg cagcgttaaa tgaagaaata gtgcgacaaa gccaaccatt ggaacaagaa
     5881 ctcgtggtgt tactggaacg aaaggaagaa ttgaagacta aaattgaaaa atgggaagct
     5941 gctttggaag atagtcctga attgtttccg atgttaaaag accgcttaga cgaattgact
     6001 gaaaaacgac gacagttgca cattcgagaa aatgaaatct tagggatctt tcaacaagga
     6061 gagccgattc aagtcaaaga tgtccaacgg atattgacta gtttggatcg ttttcttgcc
     6121 cagagtgaga agaagcaaat caaagccttg tatcgtacct ttatagagaa aataaccttt
     6181 gatcctaacc ataaggagat gttggaaatt acgatgaaat tcacgcatgc cgtggtacaa
     6241 caattgaatg agcaatatca aatagccgta tccaaaacaa aggatacggc tatttttgtg
     6301 ttaaaaacac cctttacgtt aacgatctag tctaggttat atcaaacaac gttagagggg
     6361 cgctaacgct tctctagcga tttgaaatcg ttagagaagt agttagggag gtccttatca
     6421 taaataggga tatttattaa tgaatatttg aaatataatt gtatataaga attaaaaaga
     6481 gtggggatag atatgaaaaa gatgggtaag tgtattttat gtgaaaaata cactgaactt
     6541 actaaagaac atgtaccacc taaaaaatca gggaatactg gaggtaaaaa gaccagaact
     6601 ggtaatttag atgatttcct taagagcgat tttacaaaag gtgattttcc gaaggggatt
     6661 aaaaggaaac ctcaaggaaa tgtttattat a
//
LOCUS       DACCXW010000135         6382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCXW010000135 DACCXW010000000
VERSION     DACCXW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.141"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(48..1178)
                     /locus_tag="IVY71_002475"
     CDS             complement(48..1178)
                     /locus_tag="IVY71_002475"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7986503.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1827..3029)
                     /locus_tag="IVY71_002476"
     CDS             complement(1827..3029)
                     /locus_tag="IVY71_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986504.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3089..3292)
                     /locus_tag="IVY71_002477"
     CDS             complement(3089..3292)
                     /locus_tag="IVY71_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7986505.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3434..4666)
                     /locus_tag="IVY71_002478"
                     /pseudo
     CDS             complement(3434..4666)
                     /locus_tag="IVY71_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4829..5641
                     /locus_tag="IVY71_002479"
     CDS             4829..5641
                     /locus_tag="IVY71_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986506.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1902 a         1309 c         1003 g         2168 t
ORIGIN      
        1 gaaaagccga ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact
       61 tcttttgcga cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg
      121 tttccttctt ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt
      181 acaaattgtt gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga
      241 aacttagaag taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa
      301 ggagtaaaat ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt
      361 gaaaacaact cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga
      421 ttcccgcggc agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta
      481 ttgataatct ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt
      541 gcttctcttt tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa
      601 cggcaacctt tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca
      661 taaaaatcag atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg
      721 atctcttgtc gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca
      781 tctgtttcca ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg
      841 aaagataatt tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt
      901 tgattatatc cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca
      961 agatgccaaa atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt
     1021 tggtcttcca ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa
     1081 agtaagcctt cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt
     1141 ccgacatgat ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt
     1201 ttttgtaaaa aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg
     1261 taccaccttt tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct
     1321 tggtaacgaa agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt
     1381 ttcgacagtc aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt
     1441 ctactttcct tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa
     1501 agtcaatagg gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg
     1561 gaatacaata aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac
     1621 ttccttaaaa tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt
     1681 tcccatcaaa actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat
     1741 ttccgataca tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt
     1801 tgtcttatgg tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga
     1861 atagcgccct cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat
     1921 aagtgaccgt aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata
     1981 gatagcggtt gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga
     2041 agcttaatcg gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa
     2101 taaggtggcg taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt
     2161 tgcaagtcat accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga
     2221 taagcacatt tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat
     2281 gtcttctcaa tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca
     2341 agttctccaa tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg
     2401 gtttgaatct tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa
     2461 aactcaactt tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca
     2521 atcaccccct ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg
     2581 atataagatt ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc
     2641 tggcttagtt caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa
     2701 tgatagaaaa actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt
     2761 ttgacctgtc tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt
     2821 tgattggtag aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac
     2881 ttgtcataag caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc
     2941 tttcttccat tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta
     3001 tcttggtaaa tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc
     3061 tactttaata gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt
     3121 caattactcg ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa
     3181 tccttgcaag atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc
     3241 ccatctctat caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac
     3301 ctcctgattc tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga
     3361 tggttgtgtt atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca
     3421 gtactttgct tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat
     3481 aatactcaac ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga
     3541 tctgtcttgg attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg
     3601 tttgatcgac ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca
     3661 tagttttctc aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc
     3721 taataaaggc tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat
     3781 aaaaacaaat tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg
     3841 aaataatctt tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct
     3901 ttacttttgc ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat
     3961 aaaagcaaaa acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt
     4021 cattgtttat gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat
     4081 ggcctagtat taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta
     4141 aagtaaaatc aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt
     4201 gccaatttgt ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa
     4261 actccatcac agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat
     4321 tccacacgta ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa
     4381 tgggcatttc aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg
     4441 ttagataatc aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca
     4501 caagattcaa tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact
     4561 atctcgaaat ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag
     4621 aaactcgaat ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt
     4681 ctgctattta cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc
     4741 ctgtatttat tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt
     4801 taagaactta tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg
     4861 aattcggcag attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg
     4921 tgatttaccg cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga
     4981 aacccttcac caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga
     5041 tcaagagaat caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa
     5101 gattgaaggt ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat
     5161 ggatcgaatg attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt
     5221 tactttccaa tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa
     5281 ggaagcccag ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt
     5341 taccttctcc aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta
     5401 ttcctatttt gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc
     5461 aattaatcaa ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc
     5521 ttcttatcag tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc
     5581 attcgtgatg gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg
     5641 aggtgacaaa attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg
     5701 tagttgtcac agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct
     5761 gcagtccggc tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg
     5821 cgaaacccga cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact
     5881 gatattctca taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga
     5941 taacccgctg gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat
     6001 atacatctca acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt
     6061 accagtgcat tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt
     6121 ggcggaaatt tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt
     6181 attgcaatga aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta
     6241 tctacctctc aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca
     6301 gtgatgattc ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa
     6361 gaaatagttt cttatttttc ac
//
LOCUS       DACCXW010000136         6297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCXW010000136 DACCXW010000000
VERSION     DACCXW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.142"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..316)
                     /locus_tag="IVY71_002480"
     CDS             complement(<1..316)
                     /locus_tag="IVY71_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HAP7986507.1"
                     /translation="MNAFKTALNNNEMEGKIIGADCSELAPALYMCDEKIVLPKVSES
                     NYIEELIKVIKFFEIKLIVPTIDTELSTLSRNKKYIENETDSVVLVSSEKVIDICENK
                     ILT"
     gene            complement(370..2187)
                     /locus_tag="IVY71_002481"
     CDS             complement(370..2187)
                     /locus_tag="IVY71_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7986508.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2201..2965)
                     /locus_tag="IVY71_002482"
     CDS             complement(2201..2965)
                     /locus_tag="IVY71_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7986509.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2993..3691)
                     /locus_tag="IVY71_002483"
     CDS             complement(2993..3691)
                     /locus_tag="IVY71_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7986510.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3703..4482)
                     /locus_tag="IVY71_002484"
     CDS             complement(3703..4482)
                     /locus_tag="IVY71_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7986511.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4498..5442)
                     /locus_tag="IVY71_002485"
     CDS             complement(4498..5442)
                     /locus_tag="IVY71_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7986512.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5488..6267)
                     /locus_tag="IVY71_002486"
     CDS             complement(5488..6267)
                     /locus_tag="IVY71_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986513.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1639 a         1308 c         1136 g         2214 t
ORIGIN      
        1 aagttaaaat tttgttctca caaatgtcga tgactttttc tgaagaaact aatacaactg
       61 agtctgtttc attttcaata tattttttat ttcttgataa tgttgataat tcagtatcaa
      121 tagtaggaac aattaattta atttcaaaaa actttattac ttttattaat tcctcaatat
      181 aattactttc tgaaacttta ggtaatacta ttttttcgtc acacatatat agtgcgggtg
      241 ctagttcaga gcaatctgct ccaatgattt ttccttccat ttcattattg tttaatgctg
      301 ttttaaatgc attcactagt tcacaacgac gtccacaact taatattaaa atattcaatt
      361 tttaatctcc tatctattcg aagcatttgc aaaagtgaca atgtctttag ccaattgttc
      421 atcgtcttgc ggcaggctct tgacaaaatc catcactttt tgtattgagt aacctttgat
      481 gtttcctaca aagattttct cataaactgc ttcatcattt ctttccttat ccaacaatag
      541 ttcttcatat agtttttccc ctggacggat acctgtttca atgatttcga tttcgtcttc
      601 gctatacccg ctcaatcgga tcatgttttt cgctaggtct acaatcttca ctggttcact
      661 catatctaga ataaaaattt cgccaccttt tgctaatgcg ccagattgaa taactaaacg
      721 actagcttca ggaatcgtca taaagtatcg agtcatacga aagtctgtta cagtgattgg
      781 accaccttga gcaatttgtt ctctgaatac tggaatcaca ctaccgcgag aaccaaggac
      841 attcccaaat ctgacagctg agaatttcgt acagccttct tcattcaatc cagtcacaat
      901 catttcagcg attcgttttg ttgaacccat gacatttgga ggattgttag ctttatctgt
      961 cgataccatg acgaagtttt tcacttttgc tgctttagct gcttctgcta cattttttgt
     1021 accgaaaata ttgtttttga ctgcttcttt tggattatat tccatcagtg gtacatgttt
     1081 atgagcagct gcatggtaga cgatatctgg atgatattgt tccatgatct cattgatttt
     1141 ctcgcgatct tggatatctg cgatgatcgg tacgatttct gtaggacaat tttgatgatg
     1201 cgtgcgtaac tcacggtcaa tcaagtagat cgagttctcc ccatgtccta aaagcaatag
     1261 ctttgcagga ttgaattgaa tgatttgccg acagatttct gaaccgatcg atcctcctgc
     1321 gccggtgaca agaatgactt tgtctgtgat ctgatctttg atggattcaa tatccagctt
     1381 tacctcatct cttcccagta aatccactac gtcaattgtt tttaattttg atacgctaat
     1441 tttccctgaa gcaagttctt ctatagaagg catcgtgttg actttcacac gagcggattc
     1501 gactaactcg aagattcgtc gtaatttttt tcttgataag gaagggatag cgatcgttac
     1561 catttgaatg tcatattttt caatcaattc cggaatatct tttaatgcac ctaatacttt
     1621 ttttcctgaa agatacgtat ttcttttatt tggatcatca tctacaaacc cgacaacgtg
     1681 gatatcttga gccgttttgg atccaagaaa gctgttgtat aaaatacgcc cgccttcgcc
     1741 agcaccaaca atcaatgtat ttttagcaga atctgctgat acgtatctca tattttttgt
     1801 ttcgatgtag atacgccaga tcaatcggct accgatgatc aataagagcg aaagcagata
     1861 agcaaagata actaaccgta aactgtatcg ccgattaatg aacagcagta tgaggatact
     1921 tgaggcagca cttgcagata gtgaactaaa aattgcaatg atctctctta aatttgtata
     1981 gcgattgatc cgagtgaaaa ctttgaataa actaccataa aataaataaa aaccaatcga
     2041 caaaccgata gagattaaaa taagatccat tggtatagat acaaacggct tcatgaattt
     2101 agctgctgca atatttgcag caattaataa caagctgtcc acaaaaacca atgtggcaat
     2161 ttttcttctt cttgttaata taaacacttt ttaattcccc ctaaaataaa ccaaacttct
     2221 tcttttttac ctcttcatag tctggataac tgatttcatc cccgttgatc agatcctttg
     2281 caacttgctt catctgtact actttttcct taccaaagtc cttggcaatc tgctcatagc
     2341 actcttttaa gtaaaaggtg cgcttagaga ctccgtgtgc atcagaagca gtcatctgca
     2401 cgagattatg tttgaccatt gttttagctg ttttctgaat atctttgcca aataccccaa
     2461 cataacttgg cgcagtaagt tgggccaaac accccatatc taggaacgga agcaaatgat
     2521 tcgggtcttc gcgaaaatgc gcgttgcgtt ctggatggac gatcactggt gtctttccta
     2581 gttctaacaa ctcaaaaaaa agccgttctg catataacgg cacttccagt gtcggaaatt
     2641 cgatcagaag atatgtatca tctaaatctg taaataaaat ttcatctcgg tggatgtctt
     2701 cgatgagcgt tccggtaata cgtacttctt gtccttccaa taacgtcaaa ggcaactgtc
     2761 ttttctctaa ttcggcttgt aacgaagcga ctagagaaat gacttgtgat ttttcattgc
     2821 tgtattttcc attattgtgg tgtggtgtgc ataaaatatg cgtgatgcct tgctgaatcg
     2881 ctttttcagc cattgcaata cttgcttcta agttttctgc cccgtcatcg attcctggta
     2941 ggatatggca atgtagatcg atcatttctc ttgtctcctc tcttcttatt ccttaatttc
     3001 cgtagtaata accagcatct ttgctatgtt ctgctccgtt gtatacaaca cctaaaactc
     3061 gtgcttgtac catctctagc agttcttttg ctttattcaa tgattcttta cgtgatgtat
     3121 tttcacgtac gaccaataaa gtgccatccg tttttgaaga catgatctgt gcatctgtta
     3181 cggctactac tggtggcata tcgaatatca ccacgtcaaa taactgtctt gcttcgtcta
     3241 ggatctgatc cattcttggc gaacttaaca attctgatgg gttgggtggc ttaggtccac
     3301 taggcaaaat cgacaagttc tcgactggcg ttcgttggat tacatctgct acacttcctg
     3361 atgaactaag tgctgtactt aatcccctgg cattgttcag ctggaatgtc ttatagacga
     3421 cgggtttacg taaatctgca tcgactagta gaactcgttg accggattta gcaaaaacga
     3481 ctgcaatgtt tgctgcagtg gtggattttc cttctccagg acctgatgaa gtcacgacaa
     3541 tcgttttgat ttgctgacca gctgcggagg caaactggat attcgtccgg atcgtacgat
     3601 attgttcggc gattggtgaa gatgggtttg ttaatgtgat caaactgact gcatgagttt
     3661 gtgctgcttt ttgtttctgt gttcgtgcca tttttcttcc tcctagactc ttgagcggct
     3721 tctgcgtgat ggggctgttt tatcgtttgt agcttttggt cgtgcaggag ctgttccttt
     3781 tttacggata ccagcgttca attctttcgc tgacatttct ggaactgttc ctaagattgg
     3841 gaatcctaat gcatccgtca catatttttc atctttcact gtccgatcaa gtaattcgaa
     3901 taagaatgcc agacctacgc ctaccatcat tcctaatact aagccaatca ctagattcaa
     3961 tttattattt ggcgaaacag gtgtcgtatt ggcaactgca cctgaaataa tcgagatttt
     4021 atcgacattc atcacgtctt ttgcgttctc ttggaaaact tgtgaagttg tgttagcgat
     4081 ctgttgagca gtcactgcat tcgtgcttat tgcctggatc gagaacattt gtgaattttg
     4141 tgactgattg acggagatcg catctttgat ttgtccagca gtcattttca cattgtcttc
     4201 tgtttctaaa cgatctttga cttcatttag tactaaatca ctgacgatca tatctttata
     4261 cgtattgatc atttgcaggt tcgtattgac atcattgaca tttgctgtgt ctgattgtgg
     4321 cagtgtcacg atcaattgtg cttgtgaact gtattttggt gtcaagatga aaaatgttac
     4381 gattcctgag atggccaagc cagcaaacat tgagatgatg attgttacca agtgtttgcg
     4441 taatgtttca aaaatttctt tcaagctgat tgtctcttcc attttttctc cactttccta
     4501 tttattctct agattcattt gatcttttaa ttcttttttc acacgatcaa gttcttgatc
     4561 gctgactttt tgataggaga tattatcttg catgaagcct tctccttgca gctgatcttg
     4621 cttgatcgta ccaaatgcct cccgataatc taaagcgatt tttttcattt catcaaagct
     4681 catgtctgtt ttcatattcg actctagagc attcaaaacg gattgatagt tgctgacacc
     4741 gtttaaactc aatacttttt ttgctacacc ttctacgatc tttctttgac gctcttgtct
     4801 accatagtca ccatttggat cttcataacg catacgagaa taagataatg cctgatcccc
     4861 gttcaatgta attttaccga ttggaaagtc atagccatct tgtgagaaag tgaggtcatt
     4921 attgacttcg ataccaccaa ctgcatcaac gagttccttt agccctttca tattgataga
     4981 gacatagtga tcgattggga tgtctacata tttttgtagg gtatccatcg ccatggcagc
     5041 tccgccgaat gcgtaagcgt ggttgatctt gtcttttgtg tcacgaccta cgatgtcgac
     5101 atacgtatct cgaggaatgc tgacgatcgt tgtctgtttg tcttcaggac tgactgtggc
     5161 aaccatcatc gtatctgatc ttccttgatc gacccgacct aaatctccag tatcaatgcc
     5221 catcaataga acagagaaag attcttgttt gtccagatca actggtttcg ttcgttctcc
     5281 ttgggttcgt tccactgatt cgtaagtttt ctcaatagaa tgtgagagat ccagatataa
     5341 ttttgctcct actcctacaa ttgctaatac taatacgata agaactgcta aaataatcag
     5401 aaatattttt ttccctcttg attttcttct tcgtcttgcc atagtttcct ccgtttgcta
     5461 accttgcttg aatcaacgtt ttaactgtca ataacttata ttgctatagc ccatttgctg
     5521 agcaacagtt tgtgcttttg ttgcgtatgc tgcgcttatt ttttcattcc ggatctgctc
     5581 tatttgactg agcgccgtca aatattgatc gtaagtagcg gtggcagtca cttgaccatc
     5641 cttcaacatc gtatcgaata actgatctag ttgcgtgact tgcgcgatct gagtatcagc
     5701 gaatcctaga tattgcataa caaccgtttt ccaaggacta tctttaaaaa agctcatatt
     5761 ttcacgtaca cgagcgacat ctgcacttgt caccttttct ttgatgacga catcgtctac
     5821 agcttgctgc cagtttgata catttttagt aaataaatcg ttgacagctg tctggatctt
     5881 cagttttgat tctgcactat ctaactgtgt cacgattgct ttcttctcat cttggattgc
     5941 aacagcttct ttaggcaatt ctgattcttt gatgtcgtaa tcatctgctg aaagtttgac
     6001 agaggatagc ttatttgaca aacttgtgat tgtttcttct gttatttcag ggtttagaaa
     6061 atttttttct tttgtactat acagcttgtc tacttctttt gataaagaag tcaaggtgtc
     6121 tttttgattg gtgatggcag attctgcata attgatccgt tgctgatgtt gccgctgatt
     6181 attgatatgg taaccaaaca aacagatcgc aatagcacct gcacctaccg ccacagcagt
     6241 caatgacttg acgaatggct tttccataga ttcactccta agtattgatg atagata
//
LOCUS       DACCXW010000137         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCXW010000137 DACCXW010000000
VERSION     DACCXW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.143"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            725..811
                     /locus_tag="IVY71_002487"
     CDS             725..811
                     /locus_tag="IVY71_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7986514.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVY71_002488"
     CDS             complement(932..1621)
                     /locus_tag="IVY71_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986515.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IVY71_002489"
     CDS             complement(1628..1906)
                     /locus_tag="IVY71_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986516.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IVY71_002490"
     CDS             complement(1866..2780)
                     /locus_tag="IVY71_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7986517.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVY71_002491"
     CDS             complement(2762..3142)
                     /locus_tag="IVY71_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7986518.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVY71_002492"
     CDS             complement(3592..3879)
                     /locus_tag="IVY71_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7986519.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVY71_002493"
     CDS             complement(3899..4123)
                     /locus_tag="IVY71_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP7986520.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4793)
                     /locus_tag="IVY71_002494"
     CDS             complement(4578..4793)
                     /locus_tag="IVY71_002494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986521.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="IVY71_002495"
     CDS             complement(4763..5296)
                     /locus_tag="IVY71_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7986522.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVY71_002496"
     CDS             complement(5317..6159)
                     /locus_tag="IVY71_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7986523.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACCXW010000138         6124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCXW010000138 DACCXW010000000
VERSION     DACCXW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.144"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            67..912
                     /locus_tag="IVY71_002497"
     CDS             67..912
                     /locus_tag="IVY71_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7986524.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1193..2413
                     /locus_tag="IVY71_002498"
     CDS             1193..2413
                     /locus_tag="IVY71_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7986525.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2410..5130
                     /locus_tag="IVY71_002499"
     CDS             2410..5130
                     /locus_tag="IVY71_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7986526.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5114..6058
                     /locus_tag="IVY71_002500"
     CDS             5114..6058
                     /locus_tag="IVY71_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7986527.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2286 a          961 c         1197 g         1680 t
ORIGIN      
        1 gtatatgttt tgtgagaaat ttagacttac aataagcagt gaatttataa tcaacgtata
       61 ggagacatgg atgaaatgaa actaattggt atagatttag acggtactct tttgaattct
      121 gaccagagca ttagtcaaaa aaatgcaaga acaatgaaac atttaccgga agactgtttt
      181 ccattcattt gttctggaag agaagtagaa gacattaata atattctcaa aaaatctggt
      241 ttaaggatac cagcagtagg gttaaatggt tctgttggat ttgatggaga aaaaaagata
      301 tttgagtctt ccttcgataa aaatgatgta aaaaaaattg attctgtcct atcgaaattt
      361 ccaaccaaaa tatatacaaa tctcggaagt tatgaatctc agaattataa aaataaaatg
      421 aaaaaaattt ttcacgaggt agggagtgaa ttttctatca aagagctaaa ctatgagctt
      481 gagtatgaaa aatctatttg ttcaatccct tataataata ttgaggaaat tatagaaaag
      541 gatgctatca gcatttacaa attttttatt tttattccta atcgtcaatt gaaagctaat
      601 atatttaacc atttgaaaaa gatgctaaat gtgtctgtta ctgaatcggc acctgtaaat
      661 attgaaatcg tacctcaaaa tgtatctaaa ggtgtggtgt ttgatcgttt ggaatcaatc
      721 tacaatttaa agaaaccttt gagaatcgcg atcggagata gtttaaatga cttatctatg
      781 tttgaaagtg cagatattag ttttgcaatg ggaaatagtc atcaagaaat catgaaaaaa
      841 gctgattatg tcactgtttc aaatgataaa gacggagtat ctcaagcatt tgaaagaatt
      901 cttaccatat aaactaatta aggtagtata gaacatagaa ttctaaatac ggaaaaatgg
      961 agcataaatt ccagtacaaa tagtacttct tcagtgtcaa acgaagcgaa tataacatag
     1021 atatttagga ttgtgacaaa aatcatgtca caatcttttt ttcgaataaa cttatttttt
     1081 gaagtcggcc atttatcgta atttctttgc ataatacgaa caaattttcg gttattgatg
     1141 aaaaaaaaca ttgaaaatgg tatgataaaa gaaaaaaagg aggaaatttt taatggtgaa
     1201 atttatccat gcggcagatt tacatatgga tcggtcattt gaaggactga caactcttga
     1261 taaaactgta caggaaaaac tattgaagac gaatctgacc gttttatcaa atatcattga
     1321 acaggcaata ataaataacg ttgattttgt tttacttgca ggagataatt ttcatcaaaa
     1381 tcgtccttca ttaaaaatac agaaacattt ttcagaacag atgaaacggt tagaaaagaa
     1441 ccatatacct gtatttataa tattcggaaa tcatgatttc tatcaaaaag agcggtattg
     1501 gttctccttt cctaaaaatg tccatttatt tacatccgaa acggtagaga caaaaaaaat
     1561 aacgataaaa agtggagaaa ctgtctctct cagtggattt agttatcgtc aaccgtggat
     1621 tcaaaaagac aaagtgatgg aatttccttc tagagaactc acagattacc atattgggtt
     1681 gtaccacggg gaacctggcg tctctcaaaa aggaaactat gctcctttcc aaccctcaaa
     1741 gatgcaagaa aagggatacg attattgggc tttagggcat atccatgtgc caacggtctt
     1801 aaatgaaaag caaacaattg tttatccagg tgcgcctcaa ggtcatacta aaaaggaaca
     1861 agcttctaca tctattctac tagttgaatt aagtaaagga agttgccaaa tacatccgat
     1921 taaagtagca gaggtctatt ggaagacaaa ggaaatatca ttgaggaaag caagaacgac
     1981 aaaagaggtc attgttcaca ttcgtcagca gctttcaaaa gtagaggatg gatttagttt
     2041 aattgagata aaattaaaag attatgacca tctcaataca gatgttttgg aacggataca
     2101 atcaggagag ctattagact atttgttaga ggaattctca gataggataa atgatttctt
     2161 tatttggcgt atttctctca tagagaatac tttttatacg aagaccccac ttgctgcttc
     2221 tgctaaattg atggaacagt tatttcaata ctatcagaca cctcaagatt tccaacaaat
     2281 tttaaatgaa gtatatagtc atcaagaagc ggctagaata ttgagcgaac tgccggaata
     2341 tcaagaagaa acacttgaaa aagccaagca gctgcttaat caagattttt tatttgagga
     2401 ggatcaggaa tgaagatttt aaggatcgag attgctgctt ttggaaaatg gcgtcaaaaa
     2461 agttttgact tttattcagg caatcagctg atctatgggg gaaacgaagc aggaaaatca
     2521 acgatttatc aatttattca agcaatatta tttggtttcc cttcaaaagg ccgtaaaaaa
     2581 aaggattata caccaaaaga cggctcagcc tatggcggca aaatatggct taaacatcct
     2641 gtatatggtg aatttgctgt cgagcgctac aagcagcaaa atcgcggaaa atcgaaggtg
     2701 tggctggggg atcaagtggg aagtgatgag ttactagaaa aactgatcgc acctttgacg
     2761 aaagagttat tccagcaggt atttactttc cagcaagagc agttgatgga actagatcat
     2821 ttgagagaaa aagagctaca tgatgcgctg atttctttag gcatcacggg cagttctcaa
     2881 gtattccaaa agcggcaaga atattatcag aaagcacaac aattattcaa gaaaaaaggc
     2941 caaaaattac taatcaatca aaaattaaat gaatggaaag agctgcaaga aaaaattcat
     3001 caaaaacaag aacaggaagc ttattttggc aagttgatcc aacaagaaca ggaatacgag
     3061 tcgcaacagc agcagttatt ggcacaatta aaagaggcga acaaacaatt ttcattagcc
     3121 agacagcaag aacttaattt ttctttatat gaagaatggc aatctctcaa aaaaaacagc
     3181 actacaggtc ttcctgatga gcaacttctt ttagatctgg agtctttctc caaacggtat
     3241 cagcaaacaa atgagcgttt ggaagaatta gaagcaacac tggaaaagca tagcgggatg
     3301 gatcagcagt cagccagata ttatttttat ttagagcagg agcaggaact aaaagatctc
     3361 cagcaacttc aatttacagc ggaacaatta aacgaagaat ggcaaagatt acaaattgag
     3421 actagagaaa caaattcaga attgaagatt ttggaacaaa ggtggcattg gcagaaaaat
     3481 ccgttaccgg aagaattttt atatgaagaa gaatggaagc atcttctggc tgaagaaaga
     3541 aggattgctg atctagaaag ccagatttct gtgcgtctcc aaatagctaa agaacagcaa
     3601 gagatgattg aagcagaagt gaatgacttg gaagagacct atccagcatt attacaaaat
     3661 cagtcagaga tagcaccagt ctcttcatct aaaccgtata tgctgatggc tggcggagca
     3721 ataatcattg caggtatatt tgtaccaatg cccttgaaag tgatcctttt actaattggt
     3781 gcaggcatga ttggctattt cttctacaaa aggcaaccaa aaattaaaac taaaaatgat
     3841 ttctctgaga taaaaggaat gtggcaagaa aaactaggac aacttgatct agccttagct
     3901 caattagctg aagcagaaga aaaacaaaag agaactggag aagaaaaaca gattcttcag
     3961 caagaagtat atcgttttgc ggatcatcgg cacttaggaa agatggatac gctcaaactc
     4021 atgaaagaat atggacagga agtcatagat tatcagaaat tatatggcgt atttgagcgg
     4081 aaacataaaa gagaacagga aatacaagaa aaactacgac aaatcgacca aaaatttgag
     4141 tttgttaaag aatggttacc gatacaagat aagacaattt cagaaaaaat ggtattgctt
     4201 tcccgtttta tacaagagat ggaacagatg aaatttgcca gaagctatca gcaaaatact
     4261 ttattgaagc aagaaatcaa tcaattgaaa aagaaacaaa aagtatggat acaagaatac
     4321 agcgaacaat tgactcaagc aggcttatct tatccttcgg aagtacctgt ctttttacaa
     4381 aaagccaaac aagccgaaca gcagatgaaa agatacaatg aattatcgga aatgcttact
     4441 ccactatttc ctgaagagct gacaaaagag aaattatctg taagactgcg ccaactgaaa
     4501 gaaaagcaat cccaactcca gcaagaagaa aaagaagcaa gtgaaaatcg gcaacgactc
     4561 caacttcaag tagaagagtt gcaaagagac ggcacattgg acgaacttta tcaacaagct
     4621 agccggcaaa aagcaaaatt acaggaatta ctggaggaat ggggagcact tgaaatcgcc
     4681 gggacattct taggtgatct ttcaacagag atgagtgaaa gacaattacc ccagttgtta
     4741 agagcagcag gtgattattt aggtatttta acaaaaggaa attatcagaa gatattactg
     4801 aaaaatgata cactgacttt aacagacgga tatacagaat ttcccatcta tgagttatct
     4861 acaggtacga aagatcagct gattatggcg atgagatttg cttacctctc attagaagct
     4921 aaccgttcga tatgcccgat catcattgat gatggctggc ttcattatga tcatcagcga
     4981 aagtatcaat tagctgaact tttagctaaa tttggagaaa agtatcaaat aatttgtttt
     5041 tcttctgata aagaaatggt aagctattac catgagatga accaaatcgt aaaacaatta
     5101 gaaggagcaa ataatgaaaa aaattcgtga tttagtaatc gatgagcagt ttgaacaatt
     5161 tgtcctgatc aaaagtgcag atgtaagaat cgctaaaaac ggaaaaaaat ttattgcttt
     5221 tactttccaa gatacttcag gtactatcga tggtaaatac tgggatgctt cggaggagga
     5281 aatcaataaa tttacagccg gcacagtcgt ttttctaaac ggtaaaagag aggtttatca
     5341 aggaaaccct caagtaaaga ttcttcatat gcgcttaaca agggtagatg agccaggaga
     5401 gcctagttta tacatggaaa gagccccttt gaagaaagaa gaaatggaag aagaaatcaa
     5461 tcaattgttg tttgagatca ccaatgcgca ttggaatcgt attgttcgct atctacttaa
     5521 taaatatcaa aaacaatttt ttgagtttcc ggcagcaaaa agaaaccatc atgcatttgc
     5581 cagtggatta gcttatcata ctttgacaat gcttcactta gggaaatcaa tcgccgatga
     5641 atatcctgag ctgaatcgag cattgttgta tgctggtatc attttgcatg atttaggaaa
     5701 ggtgattgaa ctttccggtc ctatgtcgac agaatatacg cttgcaggaa atttgatcgg
     5761 tcatctggta ttgattgacg aagaaataac taaagcctgt cttgcgttga aaatcagcga
     5821 agctgacgaa gatgttctcg tattacgtca tatggtttta gcacaccatg gcttattaga
     5881 atatggctca cctgtacgtc caagaataat ggaagcagaa atattacatc aaatcgacaa
     5941 tatcgatgct tcgatgcaga tgatgttgac ttcgattcgt caaacagaac caggaaaata
     6001 caccgatcgg attttcggta tggacaaccg aagtttctat gtaccaaaag atttatagca
     6061 aaaatttatt tttttcattc aaaacaaaaa aagcgagaca taagtcttcg ctttttttgt
     6121 tttc
//
LOCUS       DACCXW010000139         6122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCXW010000139 DACCXW010000000
VERSION     DACCXW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.145"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            172..1017
                     /locus_tag="IVY71_002501"
     CDS             172..1017
                     /locus_tag="IVY71_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7986528.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1208..2395
                     /locus_tag="IVY71_002502"
     CDS             1208..2395
                     /locus_tag="IVY71_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7986529.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2401..2757
                     /locus_tag="IVY71_002503"
     CDS             2401..2757
                     /locus_tag="IVY71_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7986530.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2759..3097
                     /locus_tag="IVY71_002504"
     CDS             2759..3097
                     /locus_tag="IVY71_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7986531.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            3492..4526
                     /locus_tag="IVY71_002505"
     CDS             3492..4526
                     /locus_tag="IVY71_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7986532.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4519..5205
                     /locus_tag="IVY71_002506"
     CDS             4519..5205
                     /locus_tag="IVY71_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7986533.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5229..6077
                     /locus_tag="IVY71_002507"
     CDS             5229..6077
                     /locus_tag="IVY71_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7986534.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         1973 a         1103 c         1372 g         1674 t
ORIGIN      
        1 atgaatagga atgtatgatt acgagcaagc agacagctga ttgtcttcat ctaagttact
       61 agttggatca gcaactgttt tgttgtacat ttctattata gatggttttt tgtatattta
      121 aatcaaatag tttttatagt aattatcata aaaagttatg gaagtggtgc catgtttaag
      181 ttattaacga cttttcgagc agtatatgaa acaagaaatt tttccagagc agcagaagta
      241 ctgtttttat ctcaaccagc agtctcgaat caaatcaaac agttggagcg ggaattgaat
      301 atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc caactaagca agccgactta
      361 ttatatgaac ggacactggt tttgttagaa gaatgggaag aaactcagca aaaattgctc
      421 aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt cccacacctt tgctgtctat
      481 cttttgcctc agttgatgaa acatctgatc aaaaggtttc ctaaagtgca ttttgagatc
      541 gaaatcacga attctcatga atcattagaa caagtagcga aacatgaatc cgattttggt
      601 tttatcgaaa agccattaga gacttccggc gtacaaagat actctttgat ggaagaccag
      661 ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg taagagaagc aaactctggt
      721 gtttttcatt acacgcagcg ttattttgaa gagatgaata tccaagataa gaagatgatc
      781 gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag gaatcggtcg ctcgattttg
      841 tctaagcgct ctgttccaga aaatgtgcca atgactccgt tgaatttcca tcgatatttt
      901 tatttcatcc aacgaaccaa tctggtttct gatcagctgc tagagattgc cgaagagatc
      961 cttcgataca atgaaagcac agaaacttct cacagcgagg agaatctcag ctggtagata
     1021 attgcatgta tcaaaaaaag ttctatgata cactaaagat aagcagtagg cttttgtact
     1081 gcatttgctc aagcaactgc acaaacggaa agttaatttt tcccaaatgt ctatggaaag
     1141 actttccatt atctaactgg accgctataa taatcagcag tatggttttt aagaaaaggg
     1201 atgaaacatg gaaaaaaaat taaaagcttc gaacgataat cgtattgatt atggcgtgat
     1261 tttaccagtc tttctgttat gtttgattgg tctgctctca ttatatgtag cacttagcca
     1321 tgatccgaac aatccagcag tcttgaaagg cgtagggatg caagttgtct ggtatttagt
     1381 tggagcggca gcaatcgttg tcatcatgca tttgaattcc aaatggatct ggaaactgac
     1441 tccttacctt tatggtttgg ggttagtggt aatgggactc ttgctcaaat tctatgatca
     1501 gaatttagct gcttcaacag ggtctaaaaa ctggtttcga ttcggtgcgt ttactttcca
     1561 gccagcagag ttgatgaaga ttgcgtacat cttgatgatg gcactgatcg taactaaaca
     1621 taatacgcaa gtaaaagaac ggacgatgaa atcggacttt tggctgattg ggaagctctt
     1681 gatcgtgaca ataccggttt tagcattgat catggcccaa gatgactttg gaacgatgct
     1741 tgtctttctt gcgatctttg gcgggatttt cctgatgtct ggaatctctt ggcgcatcat
     1801 cgcacctgta gtaatcttgg cagtagtcgt tggagcagga acgatcttct tggttacgac
     1861 tgaaggcgga agagacttgc tttataaagt tggattcaaa tcttatcagt ttgcacgtat
     1921 tgattcatgg ctggaccctt tccatgatac gtcaggaatg agttatcagc cggcccaagg
     1981 acttttggca attggtaccg gtggcttgtt tggtaaagga ttcaacgtaa gcaatatcta
     2041 tgtgcctgtt cgtgagtccg atatgatttt taccgttatc ggtgaaaact tcggctttat
     2101 cggtggtgca ttcgtcattt tcttgtattt tattttaatc tatcgtatga ttcgggtgtg
     2161 tttcgatacg aataatgaat tttacgccta tatcgcaagc ggtttgatca tgatgctgct
     2221 gttccatgtc tttgagaata ttggagcaaa tatcggatta cttcctttaa cagggatccc
     2281 attgcctttc attagtcagg gaggatcctc gattttaggg aacatgatcg gtatcgggtt
     2341 gatattatcg atgagatacc aaaatgaagc acctgcaaga tcttcaagga ggtaagaaaa
     2401 ttgtacacct tttactggta cccgaaatgt tcaacatgca aaaaagccaa agcgtggtta
     2461 gaccagcatg gcgttagtta tcaaaccgtt gatatgatca aagaagtacc gaaggcctca
     2521 acgattgaga cttggctaga aacgagtcaa gtaccaatcc gtcgcctgtt caacacaagc
     2581 gggatgaaat atcgggaact tggactgaaa gatcaagtag gcgagtttac ggcttcaaaa
     2641 gcgagccaag tcttatcgac agatggtatg ctgatcaagc gaccactgat cttacgagag
     2701 gaccagcttg ttgcaattgg attcaatgaa aaaatttatg aaggagaact gatataaaat
     2761 ggcaggcaag catttgaaga aaaaggacaa cctttggatc ttatttaatg gaaaagaata
     2821 ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga gacatcacgt ttgccaatct
     2881 gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg atcgaagttg aagcggaaaa
     2941 agcggtcaat gaatttgcca gcccgctaac gggtacagtt tcttctgtta atgataaaat
     3001 cggtgaagat atcaatatct tgaatgattc agatgaaatg aatgcgtgga tcttaagttt
     3061 caaagatgtt gatccaaaag aatttgacga attataaaat cagaaaatca cttgaaaaaa
     3121 gttgacactg attcttccag ttgctataat cataagcaat cctatatgaa acgagaaagc
     3181 tttgaaaaga agagtacatg tcacggacgt ttgaagagag ccctgtttgc tgaaaatggg
     3241 taacgaaaat gagatggaag atggtcttag agcagagtaa gcgagcgtaa gcaaacttac
     3301 tacgggagtg cccgttacag cgcgacagta tacggaaaga agctaagtcc aagtactgga
     3361 agaggctgtt attgcgaagt aacggtaaat caaggtggta acacggaagt tcaagctttc
     3421 gtccttttgc ttaaggcaag agggcgaagg cttttttgtt tgaaaaatta taagagaaag
     3481 atgtgaatgg catgtcatta atccaactaa cgaatgtcaa aaagacattc aatgggaaaa
     3541 acggaaacat ccaagccgtc aatgacgttt cactaaacgt tgaaaaaggg gatatttatg
     3601 gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt ccgtctcctg aatggacttg
     3661 agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga tattacaaaa ttaaaaaaca
     3721 aagaattacg tacattccga aagaaaatcg gtatgatctt ccagcatttc aacctgttat
     3781 ggtcaagaac tgtacttgaa aatatccagc tgccgctgga attagctggc gtaccaaaga
     3841 gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt cgggttagaa ggacgcggac
     3901 aagcatatcc ttcacaactt tccggcggac agaaacaacg cgtcggtatc gcacgagcat
     3961 tggcaaatga tcctgagatc ctgctttgtg atgaggctac tagcgcattg gacccgcaaa
     4021 caacagaaga agtattagac ttattgttag cgatcaataa gaaattgaat ttgaccatcg
     4081 tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg caacaaagtc gctgtgatgg
     4141 agttggggaa agtcgtagaa gaaggagacg tattgactgt cttcagacaa ccgaaaaaag
     4201 aagtaaccaa acgctttgta caacaagaca tcgaaccaga agctgattcg acagaactat
     4261 tagctgaact catcaaagaa aatccaagcg gccgtttggc aactttgacc tttagcgaaa
     4321 gaaatgccaa cgagccagtc atttcccaag cgattcgacg ctataacatc gatgtcaacg
     4381 ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt tggttcattg actgtcttga
     4441 tgactggtga aaccaaagaa ttagatcaag cagaagtgtt catcaaagaa caaggagtag
     4501 gaattgaggt gatccatcgt ggataaaagc ttttcagaaa catatctaga ttttaaacaa
     4561 atcaacccgg aaacgatgaa tcaggctatc cttgatacac tatatatgac attaatctca
     4621 atggtcttag tgacgatcat cggattagtt ttgggattga tcctttactc tattggacgc
     4681 aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat cgatcatcag taatattttc
     4741 cggtcaacgc catttatgat cctgatggtt ttgatcattc catttactaa agcaatcgta
     4801 ggcactatgc taggtgcgca agcagccatt ccagctcttg ttttatccgc agcaccattt
     4861 tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc ctggtgtact ggaagcagca
     4921 gatgcaatgg gagcaagtta ctgggaaatc atctggaagg tcttgatacc tgaaagtatt
     4981 cccgcattga tttctggatt aacagttaca acgatctcga tgatcggatt tactgcgatg
     5041 gccggcgcga tcggcgcagg tggacttgga ggtcttgctt ggcaggaagg ctaccagcgt
     5101 ggtaatctaa cagtgacatt cgttgcgact ttaattattt tactaatcgt atttatcgtt
     5161 caaggaatcg gtgatttctt aactaagaaa acagacaaaa gataatagga tataggggga
     5221 tgtaggatat gaaaaagaaa atttttggat ttgcagcagc gcttttgctg actgtaggat
     5281 tagcagcatg cggtaacaac agcgacagca aagattcagc aaataaagca gacgacacga
     5341 cattgaaagt cggcgcttca ccgacaccgc acgcagagat tttggaacat gtgaaaccat
     5401 tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt cgatgactat gtattaccga
     5461 accaatcttt ggaagaaggc gacattgatg ccaattactt ccagcatatt ccttatttga
     5521 acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc cggtgctgtc cacatcgaac
     5581 caatgggctt gtactctaaa aaagtcaaag atgtttccga attaaaagat ggtgcaacaa
     5641 tcattacttc aaattctgaa tcagactggg gacgcatcat cacaattcta caagatgctg
     5701 gcttagtcaa agtcaaagat ggagtagatc tggaaacagc aacattcgat gatattgaag
     5761 aaaaccctaa gaacttgaag ttcaaccata cgatcgaccc atcactttta gcttctacgt
     5821 accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa cttcgcttac ggcgcagggt
     5881 tgaatccatt agaagatgcc gtattgcttg aaaaggacaa ctctccttac gtgaatatta
     5941 ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa aaaactgatt gaagtgcttc
     6001 accgcaaaga agtccaagaa tggatcacag acaaatgggg cggctctgtt aaaccagtaa
     6061 aggctgatgc aaaataaggt atggaacaaa aaagatgaga aataagtctg taatggctta
     6121 tt
//
LOCUS       DACCXW010000140         6094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCXW010000140 DACCXW010000000
VERSION     DACCXW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.146"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            190..546
                     /locus_tag="IVY71_002508"
     CDS             190..546
                     /locus_tag="IVY71_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7986535.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            556..1455
                     /locus_tag="IVY71_002509"
     CDS             556..1455
                     /locus_tag="IVY71_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP7986536.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1972..2703
                     /locus_tag="IVY71_002510"
     CDS             1972..2703
                     /locus_tag="IVY71_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7986537.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2848..4875
                     /locus_tag="IVY71_002511"
     CDS             2848..4875
                     /locus_tag="IVY71_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP7986538.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            4983..6080
                     /locus_tag="IVY71_002512"
     CDS             4983..6080
                     /locus_tag="IVY71_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7986539.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2112 a         1007 c         1260 g         1715 t
ORIGIN      
        1 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
       61 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
      121 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
      181 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
      241 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
      301 caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa agatacacaa
      361 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
      421 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
      481 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
      541 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaat
      601 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
      661 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
      721 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
      781 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
      841 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
      901 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
      961 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     1021 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     1081 cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa caaattaaat
     1141 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     1201 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     1261 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     1321 gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg tgcccatcac
     1381 ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca agcgatgaca
     1441 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     1501 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gcggaaaata
     1561 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     1621 gtattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt ttatttaatt
     1681 ataattaata ttgattgaag tctaggcagt caagtcacat tttgatagat aggaggggga
     1741 cttacgaatt attttaaagg ctatgacagc tgtatttcta gattgtgttt gcatcaagtt
     1801 gcaataaaaa ctagaaatca aatcgggctg ataatcattt ataactaatc gatacatagg
     1861 accaaatata ggtctatgtt cttttatttc ataagtatcg taaaacagaa ttttgctatg
     1921 gattaatcca atcgactgat atgtttagta aataaagaaa gaggaatgtc aatgatgaaa
     1981 aaagaaatcg tattaacagc agcaatgatg ttattcagca tggtagcaag tacgactttt
     2041 gtttctgcaa ctgaagtata cccaaaggaa tataacacag aaggaacgat tacatttgaa
     2101 gcaggagatg agggtgtaac tcctcctgtt gatccagaga atcctgatcc caataaacct
     2161 gtcgatccat cagatcctcc aagccctgga acaggagggg cattatcgat tgattatgga
     2221 tcaaaattca aatttgggac tcaaaaaatt tctactgcgg ataaaacgta ttatgcagct
     2281 gctgatgtga tgaacgatgg gtctagaaaa ccgacatatg tccaagtgac agataggaga
     2341 agtaccttat ctgggtggaa attaagtgtc tctcaaccag aacagtttaa aactgccagt
     2401 ggggatgaac tagttggagc acagctgaaa tttacgaaag ggcaggctgt ttctttggtt
     2461 gatccaacat atacgccgca aacggtgaat tctgaattaa cgttgacacc gggaggaaac
     2521 aatacgttag caattaatgc taaatcagga acaggtgtgg ggacttgggt ctatcgattt
     2581 ggtgcaaatg aaaatgaaaa tcaagatgcg gtacagcttt cggtacccgg taaatctgtc
     2641 aagttagcgc aacaatattc cactaaatta gtttggacat tagaagatac acctaacaac
     2701 tagtttactg aaaagaactg ttaaaaataa atacgtagtc atattgtctt gcgaggtaat
     2761 cgagcaaaaa ttgtcaaagg ctaggctaaa tttgtttgga tacgaatagt tcatctagga
     2821 atttaacgat caggaggaaa agatatattg aaaaagagga ggatcgtgct cattttaagc
     2881 agtctcttag tgactaatat ctttccgatc atagggtact ctttggaatt gaatcagttg
     2941 gagcatggaa atagaagaga cgaagaaagc aaactcactt cgacattcga cagtttaata
     3001 gaatccaacc agacacaaga aagcagtgag gatttaagta tttcgagtag tggagagacc
     3061 actgacaacg tagaagctga gggttcggac aaacaaggac tagaagatgc accaatttct
     3121 gcggttgagc tgcctgacga tcccaatatt atttctatcg ataaaatttt tcaaacacct
     3181 atcggtacta gcacgtccat attggaaaat gggaagttac tgcagttgaa cccagcagaa
     3241 aggtctcaaa gaggagctat ttggtcaaat aagaagatta gtttactctc ggattttaca
     3301 tttaaatctt atttatactt aggaaatcaa cgagatagag cgggagatgg catgaccttt
     3361 acattgactg gcgatcctag aatggctagt aatcccagtc aagttattgg atcgccaggt
     3421 atgggaattg gctcatattc tactcgttct ggccaacctt acattgcgaa tgcgctctcg
     3481 attgagtttg atacgtataa aaatagtggt tcttctgatc gaatggatcg agagataact
     3541 gctgataata gagggcatgg acatgtcgct tttgtcacgc ctaaaccaaa taataataat
     3601 tattctggag agcatactgg cgtgacggta gctcctacat ttctttctaa tggcacatgg
     3661 cgcttgttaa taattaaatg ggacgcggag caaagaagtc ttacttatga tttggaaggt
     3721 gtcggtcgct cttctcggat tatacctgat ctcattaggc agtttggttc tacagaagta
     3781 aattgggggt ttacttcttc aacaggtact ttttttcaag aaaatgctgt cgctgtcacg
     3841 cgacttccat cgaatgtcac atcaacagct gatgtaaaag tcaacgatag tgattatggg
     3901 gccatcgctg aagctggagg aaacgataag ataactatca gaaatacatt gaaggtcgag
     3961 ggggattttc ttgaatataa gaaaatgcag actagtatcg acctcccaca agaattaact
     4021 attcccgagc atatcttgat caatggaaat caaattgatg ccaaagatat cttgataacc
     4081 ggaaatcgag tcacgctaaa tctgacagat tattttattg ctggagatac gttaataatc
     4141 gaattggaga caactttgca ggaagtaatt cctgaaaaag tactcactat gcattttgaa
     4201 taccttgaaa acgggaattt gattgagcaa tcgaatgaaa taaacctaaa gatcgcaaag
     4261 ctgaaggaga tagatatcca tgtttattat aaagataaag acagtcagat tgatattgct
     4321 gaaatgaaaa caatcactgg gaaaatagga gagaagtata aagaaactcc tattgatatt
     4381 gaagggtatg tttttgactc ggattcagcg aatacagaag gcatcttctc cgaaaataca
     4441 gaggatatct atttttatta cagaaaaggc aaactttatc tctcaactgc tcctcaatat
     4501 gtcaattttg gaaaacataa aatatccagt agaccattaa gtgaattcgg acagttttca
     4561 ggtgggttga aaattataga tgaacgggct tttggaagtt ggaaattaca attgaaacaa
     4621 gagaaagttc ttacaaatgg agattttgag cttccagaag ctctttcctt tgttacctct
     4681 gaaaatacaa cggttatagg gacatccgct gttacgattt tcgaaagtga tcaaaagggt
     4741 gaaagcgatt tgtcctctct tttagattct acagctcatc gtggaatcag aataaatatt
     4801 cctgtagaaa accaaagaat cggaaccttt gaggggaaac tatcttggat acttgcagat
     4861 gtcccaggaa attaacagga agagaagaat ataacaaaat tattgagttt caagaagggc
     4921 acaggtacca taggctcttg tgtcccacag agacaagatt ttgttatagt cttctcttac
     4981 atatgattaa aggagcgatt ttcttgagac caaaacaaca attatttata acactcctag
     5041 ttattttagg ggcacttttt aacaatatat cagtgtcagc tagtgcattt aatttttcag
     5101 tgacgccgat aacttcagag aatcaaatcg acaagagaaa aacttacttc gatcttcaac
     5161 ttgatccgga tcaggaagta gaagtaaaag cggaattgag aaatgataca gaaaaagaag
     5221 tgaaaatcga tatttctgtg aatagcgcaa ccaccaattc aaatgtgatg gttgaatacg
     5281 gaaaaaacga gattgaaaaa gacatgagcc tgatctttga tctggtagat tatgtgagct
     5341 atccacagat agtgacgcta aaacctaaaa gtgtccaaac cgttgctttt catgtcagga
     5401 tgccgaatga gcgatttgat ggtgtattgg ctggtgggat cacattccaa gaaatccaga
     5461 cggaaaagga ccaaacagaa actaaggatc aaagcttatc gattgagaat gaatacacct
     5521 atattattgc tttgttgatg caacagacac tgaacgaagt tgctccgaac cttactcttc
     5581 atgaagttaa accagatcaa atcaatgcaa gaaatgtcat cttagcaaac gttcaaaatg
     5641 atcaaaaaac ctatatcaat caagtagtta ttgaaacaaa aattacaaaa aaaggacatt
     5701 ctgaagtttt atatcaagag gaaaaagaag gattgcagat tgcgccgaat acaaattttt
     5761 cttttccaac tgctttaaat ggtcaaccgc taaccccagg tgaatatcac cttacaatga
     5821 ctgttttagg aaatgaaaat gaaaatggaa aattttcgag aaaaaaagga aatactacga
     5881 tcaactatac caatcaatgg gtatttgaaa aagattttac aatagacgga aaagtagcca
     5941 aggaattgaa cacgacggat gttacgatta aaaaagacaa taattggatt tatatcttag
     6001 tagcttcact tttcttattg tttattttat tatgcatact tttgtttgtt ttgagaaaaa
     6061 aagaagagaa agaaaagtaa ggttaagata acaa
//
LOCUS       DACCXW010000141         6072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCXW010000141 DACCXW010000000
VERSION     DACCXW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.147"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(76..606)
                     /locus_tag="IVY71_002513"
     CDS             complement(76..606)
                     /locus_tag="IVY71_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7986540.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(788..1444)
                     /locus_tag="IVY71_002514"
     CDS             complement(788..1444)
                     /locus_tag="IVY71_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP7986541.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1571..2044)
                     /gene="dfrF"
                     /locus_tag="IVY71_002515"
     CDS             complement(1571..2044)
                     /gene="dfrF"
                     /locus_tag="IVY71_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP7986542.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2439..3830)
                     /gene="amt"
                     /locus_tag="IVY71_002516"
     CDS             complement(2439..3830)
                     /gene="amt"
                     /locus_tag="IVY71_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7986543.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            complement(4171..4413)
                     /locus_tag="IVY71_002517"
     CDS             complement(4171..4413)
                     /locus_tag="IVY71_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7986544.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4445..5002)
                     /locus_tag="IVY71_002518"
     CDS             complement(4445..5002)
                     /locus_tag="IVY71_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7986545.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5015..5203)
                     /locus_tag="IVY71_002519"
     CDS             complement(5015..5203)
                     /locus_tag="IVY71_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7986546.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5216..5782)
                     /gene="amaP"
                     /locus_tag="IVY71_002520"
     CDS             complement(5216..5782)
                     /gene="amaP"
                     /locus_tag="IVY71_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7986547.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1828 a         1276 c          892 g         2076 t
ORIGIN      
        1 aaaaatctta aggattttcc cgccaacgtg gtcggatcgc tccgtcggtt taacagcttt
       61 tctgtttctc tctagttatc tacgcatata aatcaaatcg gttattccgt tataagtctg
      121 tgttttacaa agccttaact ttatttccct gtctatgttg cccaaaagac gaaccccatt
      181 tcctaacaga gtaggaataa cagaaatgta atatttatca atcatatttt tgcttaccaa
      241 ctgctgaaca agatttgctc cgccacaaat ccaaatatcc tttcctgctt cttgttttag
      301 ccgttccaat aataaaacag gattttcact tgtaaaatga atttgttggg aagaattgca
      361 ctctttatgt gttaatacat aagttgtaaa tttattatat acccattctt gcggagaaag
      421 ttcagtaaca acctgatgag aagtattcca ccccattaag acagtatcta tatctttggt
      481 aaattctgta taagtgtcga tgttttcatt gtcatttcca tgaccattta gccaagctac
      541 tccaccatta ccgtccgcaa tatatccgtc aaggctcatg gcaataaata aaactacttt
      601 tctcattttt tctcttttat aaatacaaac aggaatttat ttctcttttt tactcataag
      661 ggaacaactg attgctacaa ataaacccat taaaatccat ggaaatgctg ctcttataaa
      721 atcactaacc atactttatt cctccttaaa ttccaatttc cttagttcta caaactgaaa
      781 tttttgccta tgaatgtatc ttgttcttta tacgcaaaca ggcttctgtg tgcataatcc
      841 aatgtcttga aaacggcatt tgaattagtc cacaaatatc tttattacac caataatcaa
      901 tcagccaaat tgctttttca ttgtcgttta ctacattcaa cgattctaag tatccttttc
      961 tttctttcgg tatttttctt ttcaattcat cataagataa ccgttcaagc attttctcag
     1021 tgctttccca tacttcgaaa atatataaat ataattcttc cagattaagc tgttttgaaa
     1081 agtctgcaat ctgctgtttc atgagttcat ttcctgttgt aatgataggt gaattgatac
     1141 gctcttgata attgcctgca aaaaatactt gttcatcttc atttatcact gtatgcacaa
     1201 ctatatcttc aatgcggaaa atatgccaaa tagaataagc aatcgtctta ctatgataac
     1261 catcagcgtt tatgaagggg attgagttaa aatcttctct gcataattct tctttgaaag
     1321 atactaaggt ttgtattaac tgacttcgta aagtaagtaa agtatcaata cccctcttat
     1381 aagtatcttt cttttttatt tgtgcctgca ttgttttatt aagttcagac cattccttat
     1441 tcatattcaa tcctctctca aattctgatt ttatcgcccc atacaaactg gaatttgtct
     1501 tacaacaatt tatatatgca taaaaagcaa tatatttatg aaaacaccct aaatccaaaa
     1561 tttactcaat tcattttttc cttacataaa atgttctcgt atatttcact tcctcaccaa
     1621 gtgtttcccc tatcaaaact tcaaaatcat tgatatcaaa ttctggaaag aatgtatctc
     1681 catcttcaat atttaaatct acttctgtga tatacatttt atctactatt tgcaaagctt
     1741 ccttaaatag tccatatcca ccagatatgt atacatctcg tcctttagcc aataataatg
     1801 catcttctaa tgatttaact gaaactaaat tatctccttg atactctgtt gtggtggaaa
     1861 caacaatatt cattctatta ggcaacggat gaccgatttc ttcataagac tttcgcccca
     1921 taataaccac attacccgtt gttaactctc taaattgctt ttgttctccc tttattttcc
     1981 atggtatatt accattcttg cctataacat tattctttga cctcgcaaca atcaaaccta
     2041 tcatctattt catcctccat aatatgatgt cgtacctgtg attccatcaa gtgcaccaca
     2101 tcttaacaaa ctcaaaatta tctcgaacta accagctcga caaattcctt ttttgtcttc
     2161 atctgcatta tatcatattg ttataagcat tgccatttct tttctgcttt tgaaaagtta
     2221 cttatcagcc tatgttttgt aaagggtgat attcagcatt tctcacgcaa caagcccttg
     2281 acaaaccata gactaataag ttttaaggta atcaagcaga aatgaaatag tctgctgttt
     2341 cacggcaaca aaaaagagat accaacacac ttatgtagta tctcttagtc tagtagcaat
     2401 attcttttgt ttgcaagcag gtaaacatgg gctgtttctt aatatctttt tgtttgggaa
     2461 acaccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata
     2521 aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat
     2581 gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaag
     2641 aaaaagtcca atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc
     2701 gccgatttta aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca
     2761 atcacttgaa tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat
     2821 gcaaagatcc ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca
     2881 tcaagtgcat catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata
     2941 cccgcaatga tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta
     3001 atggctgcca atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct
     3061 tttacttttg caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa
     3121 ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac
     3181 cagccaaacc aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt
     3241 ctttctccta aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat
     3301 ccagctgtcg tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa
     3361 aatccgccac cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata
     3421 acaagtataa tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga
     3481 acggtaatca cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca
     3541 ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt
     3601 ccaataacac cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata
     3661 atcgttgtta tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc
     3721 cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta
     3781 cataaaatca tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc
     3841 tcctttctac gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg
     3901 aattaatact tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct
     3961 ttttcgtttt tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg
     4021 tttattttcg tcttttttta tttttaaaga tattttttca taaggaataa agaatacttc
     4081 atataaaata tataaaaaag aaccgcagcg agtgtcgcta cggttctcaa atttgttctt
     4141 catttcaaca taagtttttc ttcaatttgc ttataattta cgtgcaaaga atgaaacgac
     4201 taatactaaa atgattgcac cgatcaatga tggaatgata gccattcctg caattgttgg
     4261 tccccaagtt cctagtaaag cttgtccaat tgctgaacct actaaaccag caatgatgtt
     4321 tgcgatgatc cccattgatg atcctttatt cgtgattgct cctgcaattg ctccaatgat
     4381 accgccgacg attaatgccc aaataaatcc cattttaaat tcctcctaac tgacttctta
     4441 cttcttattg tacacgtggt tcgttatttt ctttgacttt ttgtgcacct ttgcctacag
     4501 cagattttgc tttatctgtt tggcgtgaag caaattgtcc tgtggatttt gctgcttctg
     4561 ttactttgtc ttgtactgtt tcttgatctt tttggtattc ttcttgtgtt ttgatgtctg
     4621 caacattcac attcacttcg ataacatcta aatgtgtcat attgtttact tcagtactga
     4681 tcaattcttt gatttggttg taaatatctt cgatgtcttt accgtattct acgatcacat
     4741 ctagatccac agctacttgt ttcttaccta cttctgtatt gatacctgct gttacgttat
     4801 ctgtgttgac taatttttca gccatatttg agaaaaatcc gccatctaat gtcaatagac
     4861 catctacatc tttcaatgca taaccgatga ttttttggat gactttgtct tcataagtca
     4921 attcaccatt catcatgcgg ttttctcctt tttcatttgc caaaactgat ccatttgttg
     4981 tattcatgtt tttgttttcc atctttgttt cctcctatga tttaaaaaat tgatcaacta
     5041 ttcctgactg tttcaaataa aaaccgattc ctgcgccaat tgctgtcatg attatgatca
     5101 acaatgtctt gaaaaatcca atcgttaacc ataaaacagc tagcagtagt ccaagacccc
     5161 caaagataat agggagcttg tatgctgaaa atagttcttt cattatttgg ccctcctatt
     5221 ccactcgaga gtgcttgtag tctcttgtat cttcttgttc ttcataaccg ctgtatgtga
     5281 ctgccacttt gaccgtttct tttgttccga gtactttagt tacttcttct tcgatatctt
     5341 gcatcaatac acctgttttc cctactaatg aagaagttcg tttcaagtct ccgttgacat
     5401 ggatatggat tcgatttttc gttgaacgta tccgtacttt aggtgaatga atgaactctt
     5461 cttcgtgtaa atgggaacgt accaaccctt cgatcgcttt tttgtcgatt gttagtttgc
     5521 catcttcccg tttcataacg aaagttcctc ttgactttgg atagaaaaga acgactagca
     5581 agatgataat agcaagaacg gcaaatacta cagctgccca gaaaatatat tgttgcatgt
     5641 agtaattcgt taaagtaaag aagcgaaatc gttccaattg ttcaggcata atatacggcg
     5701 cattgatcaa cgcaatcgct agaaatactg aaagtagcag taaactcatg ataacaccga
     5761 ttatttttaa ccccttattc attttccacc tcctataaac ttatttttac cctgtaaata
     5821 actctaccga caatcacagt atagatagcg aacagttagc atagcatcag taaataaagc
     5881 gctaccaaac attgctggat cataacggcg ttttattgcc agtctgatac tgaaataaca
     5941 attgcttact tattctctat ttacttttga ttgctggcta tacttaaagg atattattca
     6001 agattaaaaa ttgcaaagat tatgctcagt tattattaca agattctatt tatttattaa
     6061 attttttcgg ag
//
LOCUS       DACCXW010000142         5855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCXW010000142 DACCXW010000000
VERSION     DACCXW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.148"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(519..1022)
                     /locus_tag="IVY71_002521"
     CDS             complement(519..1022)
                     /locus_tag="IVY71_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7986548.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1381..2406
                     /locus_tag="IVY71_002522"
     CDS             1381..2406
                     /locus_tag="IVY71_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7986549.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2729..3607
                     /locus_tag="IVY71_002523"
                     /pseudo
     CDS             2729..3607
                     /locus_tag="IVY71_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4083..4199
                     /locus_tag="IVY71_002524"
     CDS             4083..4199
                     /locus_tag="IVY71_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7986550.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5127..5465
                     /locus_tag="IVY71_002525"
     CDS             5127..5465
                     /locus_tag="IVY71_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986551.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5559..5789
                     /locus_tag="IVY71_002526"
     CDS             5559..5789
                     /locus_tag="IVY71_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986552.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1987 a          882 c         1074 g         1912 t
ORIGIN      
        1 atatctcatt ttacactttt gttattttat acttaaaaaa taacgacaca gaaaataccg
       61 tgtctcagat ttctctacta cttctaaaaa ctactgtttt tttcctttga aatgccttca
      121 aaaagtagga tcaggcagaa tatactggca gaatcaagga gaagggagcc taaataagga
      181 gaaaaaccat agaaaatata aagtaattac ttataaaaca agctttttat agaaaaataa
      241 tatacctgag tagggtataa attaagacgg aattcctttc tagattgaga tattgactag
      301 gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa ccctttttgt taaataaggg
      361 gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct gcaataatat agtagattag
      421 agatgaaaat agattttttt aaaccaatga ctgaataaaa agaccgtcat agatacacat
      481 tggaatgtgt ctattacggt ctttttttac gaacgatttt attgaagacg gacagttcgg
      541 gtcaaaaata aaaaagctac gatcagaaat aagaataaaa tgtatcccaa aataatggct
      601 agacggtcta acttatttgt cgatagatgt ttgtttagaa gttgataggc ccataaacct
      661 atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt ccgctacgcc aataccgaat
      721 aggaattgaa tcaattctgc caagaagctt acaaggaaaa taatccctaa ttttttagaa
      781 aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag
      841 ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg
      901 agattgacat tgctgtaaga ataagtgaga aattgtggaa gattaaatga tagcttgaat
      961 aaaatcaacc ataatagaaa gaataaataa atccctaata aaaattttga tacataccgc
     1021 atagacatct cctccattta aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa
     1081 gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat tagaagcttt tttttgttgg
     1141 gaatccggtt tctttaacaa agctaagata gcgagttgct tggaaaagtt gctatctttt
     1201 ttctgtcaaa acgactcgga atgttcgttt tttgttctaa tttctccaag ttgtttactt
     1261 tttgatacta agtttatgaa aaatcaagaa atagttgtta tactaggtaa tgctgacaaa
     1321 cgggattggc atttcatctt ggttcagctt tttttccaat tactaggaag gagggataga
     1381 ttgttcaaga cttattttcg tttcaataaa atacaagata atccttttcg tatcatgaat
     1441 ctgatgctac ttatctttat tgtattatgg ttaggttaca taaatcatga ttttttgatt
     1501 tcaagttttt catcattagg gatctttact tttttctatt atcaaaacat accgatgaaa
     1561 tatttgctga aacgtatgtt tctgatcggt tgcggactgt tggttgcttt cgttttgggg
     1621 atcttatcga cttatgtttt atggcttgaa ccatttgccg tagctttggt tgctttttcc
     1681 agcagattcg tgttgcgatt attccatatt tctaaaccag gtggcttgtt ttttgcgatg
     1741 ctttctgcca tgggaacgag catgcagctg ccaatcgctc aattaccgat cgtctcactt
     1801 tattttttta tgggtgtcgt ttttgccctg attgcggcag tgattacaaa attactggat
     1861 tcacggcctg agcagacaat tgaaaaagca actttaaaag aaagatttca cgaagagcct
     1921 ttagtgatca ttgattctgt tttctacagc gctgccttat ttctttccgt ttatgtcagt
     1981 cacggcttga atctgcataa tccttattgg ttgaccttgt catgtgcatc gattttgcta
     2041 gcagaaaact tggatgcgat gaagcatcga caagtccaat atctgattgg atcgatgggt
     2101 gggttatgtg tgtcggcatt cttgtcattt gtgccgttta cgcagttgca gaccattttt
     2161 ctgatcactt ttctatatgg aatcgcacaa tttctagttg ctagaaatta tgcagttgct
     2221 aatatttttc ttaatccgat ggcgttgatg ctatctacac ttattcgtgg agcatacttg
     2281 atttctttga tcgaatatcg ttttttaggg atcgtcatag gaagtttcat cggtttaggt
     2341 gttgcgtggg taatgacggt tggtttgcaa cattatttaa cggttgttcg tcaaaaatta
     2401 gaatagaagg acaaatatat gtgccaaaag aggttatgac aagatttttg tcacaacctc
     2461 taattcaagg tgttcaaaag ccagattctc agcattaagt caaattcaca caaaaatgtg
     2521 gaaaggaact ttcaaaaaaa ttttatattt attctatcaa aaaattttgt ttatgatggt
     2581 aactgaataa actttcctga gatggtcaat gctttccttg aaagtcttac agatattctt
     2641 tcttcataga aagtttaagt tttatagtta gtgtacaata aaagtatgtt tataccatat
     2701 ggtgtccgga taatcagtca tttaaacaat gaggttcaaa gatatgaaaa ggggaaattt
     2761 gatgaagaaa ataagcaatg aaatccaaat tctggcagaa actttaactc ttcccaaagt
     2821 cgttatagag caaatgattg tacaagcaaa aaacatcctt gatgaggtgt ttgagcaata
     2881 tagtcatgaa ctaatggatc ctaataaagc aaaggcgact gcaaagaaaa tagacaaagt
     2941 ctatgaagag gaaccttttg cagcattagc tatttatcta tatgcagctt ctcaaagttg
     3001 gaaaaaaatt tatcaaccat tgaagattcc aagaactatc tatttggcaa caatgaatgc
     3061 ttttactaga tttattcatg aacatttcca agtaacagga agctatcgct ttgaccgtgg
     3121 tttttggatt tggcattaca ctagtggctt gatctttcgg attggtgaat tggagtacga
     3181 aatgacatat tttccgaaag ggtacacgga tctaagatta gaaggaaagc agtgcctttc
     3241 tcttcatatt ccttccgatg cagatttatc tcagaataag ataagagata gttacgaaag
     3301 tgccattcgt ttttttgaac tttattttcc tgattaccat taccaagtga tgtacacaga
     3361 tacttggtta ctatcaccca atcttactaa atagttgaaa aaagagtcaa aaatctgtct
     3421 ttttgcagcc gattatcgat tattatcagt tgatgaacag gacgatagtg gagtcccttg
     3481 gatctttgga cgagtagatg cccaaataca tgattatcca gaaagtacga gtttacagcg
     3541 acaagcaaaa gagcaattgc ttgcaggcga gcacattggc tctggcttag gtattcttac
     3601 ttcataagtt tagtacttcg aaatagttta aaagagcgtg agacaaaagt aaagaacact
     3661 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata
     3721 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
     3781 ttaaacgctt ctgtcccagc ctctttttta gtgttctatc gaacacacag tacgttcaat
     3841 aggtaaacta gctataacaa tagatattaa agttaacgga caagagggcc ttttttaggt
     3901 agctaaaacg taataatgaa aatttatttc cagagatagg agtcaatcag ctagtctgat
     3961 cgactgtttt aacaagttga ctttcatttt aacttttgat accatttaaa acacaaatcg
     4021 taattgtttc gatttgtgtt ttttagcatg actacaatca aagaagaaag tgggtcgtcc
     4081 atatggaaaa aacagatatc tcaagtgcat accgccgctt gaaaagccca aatatcaaaa
     4141 cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg gaatagtggg aaaaggtaag
     4201 aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt ttcggctatt ttcaataatc
     4261 ggtttttgtg taaggtttcc gaaagaactg ctgttgattt tccattttta acatatgata
     4321 aattggtaga atctttcttt tcaaattgat accattccct aattccagtt atatagttaa
     4381 ctacagaacc acaaagcacg tacgtttata aaagtattat ctgtagttat ttttcaattc
     4441 cctttcaacc tcttattttg accagtagta ttgatcactt taccttttaa cacctttgtt
     4501 cgtttttgaa acatttttag tcaagaatgg aaacgtaact acataatcaa aggttttgcg
     4561 aaatatttca tgaaatattt catgtagaag cgagcaatac ggttgcaaat aaccattatc
     4621 gtttaataag cagttacgaa agctcccaat atctctaacg ttcagctcac tccatttctt
     4681 ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg tcgaaaaagt taactttaaa
     4741 aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag ttactattgc tagaataaca
     4801 tcaaggaata ataagttttg gttttcagat aatagtcgat aagttgtgta ttggaatttt
     4861 agatacagta ataaagtatt tttcatgaaa ttttgtgaga atcactatta caagtatctt
     4921 taataaaatt aatggttaag gaggaactta aagtagaatg taaatgaata gttgtagtca
     4981 gtcgattgtg atactagact caaagttgca ggaataagtt tttcaatttg tgattggaag
     5041 ataaaaaatg aaaaagaagg gctttttttt atactttgtt gattgtagtg tttattttcc
     5101 ctctgtttag ccaagtaaca ttttctatga ctcaaccagt ttcaagtgag gaacatgata
     5161 acctttttcg tgctacagaa gagtttaaga aatatcagga aaatattgtt ccggagactc
     5221 cattagctag tgagccaatt actgatattg atggaaacca aataggatgg tatatacagt
     5281 acgaagttat ggtggtaaat aatgactcaa tagtaaaaaa tacagtaggc ttagagctaa
     5341 caccgattga aaaggtgagt tcattgttaa cgtatattta tactgatgaa agtgacgaga
     5401 ctgagatcat tttaacggat tactcaaaaa ttgctactga taaaaagtat atattgtgga
     5461 tgtagacact agtgaaatac tttatgatat agaaattgat gaaaatttac tagatgtagc
     5521 acaagaaatc gatcatcaaa agcaggaagc tattcgatat gctggtagaa aagggaaaag
     5581 aaaatattta ttacgtgaat gtagcaaaag ttagagaaga tgaaaatgag tggaaagaat
     5641 tcaagagtcg ctactcaatt aactcgactc cgacatttac agtttacaga gaaggatcga
     5701 tagaaaaaac tgttttttgg actaaagaaa gtggaatgtc tttagctgaa gtagaagagt
     5761 ttttggatta tgttagtatg caacagtaag ggagttctta ttatgaaaaa ttttgacaca
     5821 tattatgtta ttgttttttc gatatctttg ctgtt
//
LOCUS       DACCXW010000143         5824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCXW010000143 DACCXW010000000
VERSION     DACCXW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.149"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            379..1509
                     /locus_tag="IVY71_002527"
     CDS             379..1509
                     /locus_tag="IVY71_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7986553.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1601..2356
                     /locus_tag="IVY71_002528"
     CDS             1601..2356
                     /locus_tag="IVY71_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7986554.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2414..4453
                     /locus_tag="IVY71_002529"
     CDS             2414..4453
                     /locus_tag="IVY71_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7986555.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4562..5713)
                     /locus_tag="IVY71_002530"
     CDS             complement(4562..5713)
                     /locus_tag="IVY71_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7986556.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1993 a         1049 c         1162 g         1620 t
ORIGIN      
        1 tttcaaattc cattgcctaa tcattaggaa aagtctatca tgaactcgta aatagaatat
       61 agtaataacc ttgttaggtg aggctcctat acaaacatag gctgttgccc agaaacgtcg
      121 aaagtcgcca atgggtaaaa cagaaactgt tggattaagg ctttttccaa tagctaagaa
      181 tgttttcttt acgttgtata gtgctaaaac tcggacgatg agatgatgct aggtcagaat
      241 tcttgttcga aaaggtttct gaccttttct ttatattttt gtgactgact tatttgatga
      301 gacaaactcg ctcctcacct gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa
      361 aagatcgatt ggtggatttt gggtccttat ttgactttgt ccatgatcgg tcttttagaa
      421 gtatacagtg ccagttctta tcgtttattg caagccgatg aaaatacaaa aagtctatta
      481 ttgcgtcaac taattttcat atttttgagt tggagcgtga tcttcttagc tcgttcagtc
      541 aaactacact atttacttca ccctaaaata gcaggatacg gtttagcctt atcgattttc
      601 tttttagtat tagtaagaat agggatattc ggtgtcactg tcaacggcgc acaacgttgg
      661 atctctctgt ttggcattca attccagcct tctgaactgg caaatctttt tttgattttt
      721 tatttaagct ggttttttcg tgacggaaat agtagcccaa aagatctaaa aaaaccattc
      781 ctgattacag taggtataac ttttctgatt ttatttcagc caaagattgc tggagcattg
      841 atgatccttt cgattgcgtg ggtcatattt tgggcagcgg cggttccatt taaaaaaggg
      901 atctatctaa tcgttacttt ttctgcattg ctgattggag cagcaggcgg ggtattatat
      961 ttaggaaata aaggttggct tccacagatg tttaatcatg cttatgaaag gatcgcgact
     1021 ttaagagatt cttttatcga tagtcatggt gcgggctatc aaatgaccca ttcgttttat
     1081 gcactatata atggtggcat atttgggcgg ggattaggaa atagtatcac aaaaaaaggg
     1141 tatcttccag agacagaaac agattttatt ttttcgatta ttacagaaga gcttgggcta
     1201 atcggtgcat tgtgtgtgct tttcttattg ttctcattat gcatgagaat cttttgttta
     1261 agcagtcgct gtaaaaatca gcaagctgga ttgtttcttt taggattcgg aactttgcta
     1321 ttcgttcaga cgataatgaa cgtgggaagt atcgcggggc tgatgccaat gactggtgtg
     1381 ccattgccat ttgttagcta cggaggaaca agttatctga tcctttcttt aggaatcgga
     1441 atcacgttga atatttcttc aaagatacag gccgaagagt tggcgctcta tcgacctgaa
     1501 aaacaataaa gagaaactga gaaactctca gtttgaatta tcataaagta caatctttta
     1561 tttttcaagc gagaggttct tttagaaaga ggaattcgca atgaaagtat ctcaaaaaat
     1621 catattatct attttgttac tgcttacatt agtagttggc tatttttcga ttgagtttat
     1681 ccatggattt tcgtctgcaa aacagagttc aactgtgaaa aaggtagagc cgaaaagtat
     1741 ccctaccaca ctaaatgtgg ctttgattgg ttcagatgct cgttcgaaag acgaaaacgg
     1801 tcgctcggat tcacttctgg ttgcacaata caaccagaaa acacaacaag caaaactgat
     1861 ctctatcatg agagattcat atgtcgatat tccaggttac ggaatgaata agataaatgc
     1921 agcttactct tatgggggag ttgatttatt aaatcaaaca ttaaaggaaa atttcaaatt
     1981 tgaagctccc tattatgcaa gtatcacatt tcaagatttt atcgattgcg ttaatgaact
     2041 gtttcctggt ggagtcaaga tcgatgcaga aaaatctcta gatttagatg gcgtatatat
     2101 aaacaaagga gagcaagtga tggatggtaa tactttactc cagtatgctc gatttcggga
     2161 agacaaagaa ggagacttcg ggaggatcag aaggcagcag caagtgatca aagctatctc
     2221 tcagcagctg aaagacgtaa cttcaatatt caagctaccc aaagcggttg ggaaattact
     2281 tggtagtatc caaacgaacc ttccagaaag tgtgcttctg gattgtggta tggatttcct
     2341 caaagacata tgttagaata aacaggtata aatagtgaaa taaaggaatg acaagcaaga
     2401 gaaggaggaa aaaatgaaaa gaagtgacaa gcacggcaaa aatcgaacag gcgcttatat
     2461 tgccggcgca gtgatcttaa tagcagctgc gggtggcggt tatttttact accagcacta
     2521 tcaagaaacc caagcagtag aagctggaga aaagacagtt gagcaatttg tccaagcttt
     2581 gaacaaggga gattataaca aagctgcaga aatgacatca aaaaaggcgg caaataaaag
     2641 tgcattatct gaaaaagaga tcttagataa ataccagaat atatacggtg ctgctgatgt
     2701 caaaggactt cagatatcga atctaaaagt agataaaaaa gacgattcta cttacagttt
     2761 ttcatataaa gcaaagatga atacctcatt aggtgaattg aaagatcttt cttataaagg
     2821 aacattagac agaaatgatg gtcaaaccac gatcaactgg caacccaact tggtttttcc
     2881 agaaatggaa ggaaatgaca aagtaagcct gaccacacaa gaagcagcaa gagggaatat
     2941 tatagatcga aatggtgaac cattagcaac aacaggcaaa ctaaaacaat taggagtcgt
     3001 tccaagtaaa cttggagatg gaggcgaaaa aacagccaat atcaaagcga ttgcttcctc
     3061 cttcgactta acagaagatg ctatcaatca ggcgatttca caaagctggg tacaacccga
     3121 ttactttgtc ccattgaaaa tcattgatgg agcaacgcca gaacttccag ctggagctac
     3181 cattcaagaa gtagacggca gatattatcc tttgggtgaa gcagctgctc agctgattgg
     3241 ttacgtggga gatatcacag cagaagatat tgataagaat ccagaattaa gcagtaatgg
     3301 aaaaatcggt cgctcaggtt tggagatggc ttttgataag gatcttcgtg ggacaacagg
     3361 tgggaaatta agcattacag atgcagacgg tgtagagaaa aaagttctga tcgaacatga
     3421 agttcaaaat ggcaaagata tcaaattgac aatcgatgcg aaggcacaaa aaacagcttt
     3481 tgacagtcta ggaggaaaag ctggatcaac tgttgcgaca acgccaaaaa ccggtgatct
     3541 tcttgcgctt gctagctctc caagctatga tccaaacaaa atgacaaacg ggatctcaca
     3601 agaagattac aaagcttatg aagaaaatcc tgaacatcca ttcatcagcc gatttgcgac
     3661 aggttatgct cctggatcta cgtttaaaat gattacagca gcaatcggtc tcgacaacgg
     3721 cactatcgat ccaaatgaag tgttgacgat caacgggctt aaatggcaaa aagacagttc
     3781 ttggggatct tatcaagcaa cgcgtgtcag tagtgatgta tcacaagtag acttaaaaac
     3841 tgctttgatc tattccgata atatatatgc ggcacaagaa acgttgaaaa tgggtgagaa
     3901 aaaatttcgt acaggcttag ataaattcat ttttggtgaa gaccttgatt tgccaattag
     3961 tatgaatcca gcacaaattt ctaatgaaga tagctttaac tcagatatct tgctagctga
     4021 tactggatat ggacagggcg aacttctaat tactcctatc cagcaagcag caatgtattc
     4081 tgtttttacc aacaatggca cacttgtcta tcctaaattg attgcagata aagagacaaa
     4141 agataagaag aatgtcatca gcgaaacagc agtacaaacg atcgtgccag atctgagaga
     4201 agttgtgcaa gatgtaaatg gtacagcaca ttctctttct gctttaggga ttccgttggc
     4261 agcgaaaact ggtacagcgg aaatcaaaga aaaacaggat gtaaaggggc aagagaacag
     4321 tttcttgttt gctttcaatc ttgataacca aggttatatg atggtcagca tgttggaaaa
     4381 taaagaagat gatgattcag caaccaaacg agcatcggaa ctattgcaat acctcaacca
     4441 aaattatcaa taaaaaattg ctttttaagt acagtaaaag gactgtggcg ttatcgtcac
     4501 agtccttttt tcaaataaac ggttttcacg atgtctcttt cacaacttct ttttgattta
     4561 ctcacacttc ataaactttt tttgtaaaat atttgagaac aagtggagaa ataagtgtac
     4621 ttaataaaat cacgataact aaaggtgaat agtactgtgg ttcgatcaat tgactttgct
     4681 gtccaatttg taaaatgatc aaagccattt ctccacgcga aatcatacca gcaccgacca
     4741 tcaatgaact atgttgagaa aaaccggcaa tctgagcacc aaaataacct ccaagcaatt
     4801 tggtcaaaat cgctacaagc gtcaatatag aaataaagag tagttgttca ctaaagtcag
     4861 aaaaatcaac ttctaatcct acactgacaa aaaatactgg tataaatact gcatatccta
     4921 aagcctctac attgtggtat acttcttctt taacctttgt ctgactgaca gctatcccag
     4981 caaaaaaagc accaatgaca gagctaagcc caacaagatc cgctacgtaa gacattccta
     5041 aacaaataac tagcgatacg atgataatag aagaatttgc gtacactttt tctgcaaatg
     5101 aaagtaggaa aggcgcaatc catttgacta ataagaaaat aaaaacaaaa taaattagct
     5161 gttcaatcaa aacaagcggt aaagaagcat tgctagaagc ttcccccgtt agaaaagata
     5221 ggctgaaact tagaactaaa acgactaaaa tgtcatctac cacagaagca cccaaaattg
     5281 ttgatccttc ttttgtattc actacgttta gttcttttaa tacttctact gaaatactga
     5341 cagaagttgc cgcaagaata atcccaaaga aaatcgcttc atttcccgaa acttggaatg
     5401 ctttgcctgt caaccagcca aaaacaaccg ggaagatgat cccaagcaaa gcaacgaaca
     5461 taccaggtcg gaaatatttt ttcaaaagag ataaatcact ttccagacca gctaaaaaca
     5521 ttaataagat cacaccaatc tccgaaaagt catggactag aatatcaggt ttgacccaat
     5581 tcaatccagc ttggcctagc aaaacaccaa caagtaattg tccaatcact gctgggatac
     5641 ctaatctccg cgagatatgt gagccaatcg ttgttgaaat caatatcagg cataaaatac
     5701 caataaattc cattctcatc attcctttgt ataaaatata gcccgaactt agcataacat
     5761 acattattga aaaaatagga aaatatactc acaaaaaaac caacaatcca aaagattttt
     5821 ttgg
//
LOCUS       DACCXW010000144         5814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCXW010000144 DACCXW010000000
VERSION     DACCXW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.150"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(106..564)
                     /locus_tag="IVY71_002531"
     CDS             complement(106..564)
                     /locus_tag="IVY71_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7986557.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(577..1902)
                     /locus_tag="IVY71_002532"
     CDS             complement(577..1902)
                     /locus_tag="IVY71_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7986558.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1919..3331)
                     /locus_tag="IVY71_002533"
     CDS             complement(1919..3331)
                     /locus_tag="IVY71_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7986559.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3348..4112)
                     /locus_tag="IVY71_002534"
     CDS             complement(3348..4112)
                     /locus_tag="IVY71_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7986560.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4216..5091)
                     /locus_tag="IVY71_002535"
     CDS             complement(4216..5091)
                     /locus_tag="IVY71_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7986561.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5346..5430)
                     /locus_tag="IVY71_002536"
     tRNA            complement(5346..5430)
                     /locus_tag="IVY71_002536"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5394..5396),aa:Leu,seq:caa)
     gene            complement(5448..5519)
                     /locus_tag="IVY71_002537"
     tRNA            complement(5448..5519)
                     /locus_tag="IVY71_002537"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5485..5487),aa:Gln,seq:ttg)
     gene            complement(5529..5601)
                     /locus_tag="IVY71_002538"
     tRNA            complement(5529..5601)
                     /locus_tag="IVY71_002538"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5566..5568),aa:His,seq:gtg)
     gene            complement(5663..5747)
                     /locus_tag="IVY71_002539"
     tRNA            complement(5663..5747)
                     /locus_tag="IVY71_002539"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5711..5713),aa:Leu,seq:caa)
BASE COUNT         1769 a         1240 c          890 g         1915 t
ORIGIN      
        1 acaaaaatca aaatgattaa atctagtgat tgaaacatat catttcaaaa aaattttgta
       61 aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca
      121 actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg
      181 agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat
      241 agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact
      301 tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact
      361 ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc
      421 atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa
      481 gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat
      541 gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt
      601 tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt
      661 ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac
      721 tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg
      781 ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac
      841 tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg
      901 acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa
      961 attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt
     1021 atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc
     1081 aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa
     1141 atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt
     1201 aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc
     1261 tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata
     1321 cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga
     1381 aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc
     1441 agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg
     1501 tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc
     1561 gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc
     1621 tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc
     1681 aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat
     1741 aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat
     1801 ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata
     1861 cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct
     1921 attcttctaa taatttattg aattctgcca taacataagt cacttctaag ccgatgataa
     1981 tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct
     2041 gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta caactataga
     2101 catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat
     2161 aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg
     2221 ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa
     2281 cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga
     2341 ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat
     2401 tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca
     2461 taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag
     2521 ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt
     2581 tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata
     2641 aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt
     2701 ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt
     2761 gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc
     2821 ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa
     2881 agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt
     2941 cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc
     3001 atgccgctac taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca
     3061 taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc
     3121 ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt
     3181 ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg
     3241 ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc
     3301 caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca
     3361 tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt
     3421 aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga
     3481 tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt
     3541 aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct
     3601 cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca
     3661 aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt
     3721 tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga
     3781 taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat
     3841 ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt
     3901 ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt
     3961 tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat
     4021 gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca
     4081 aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa
     4141 taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat
     4201 agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt
     4261 ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca
     4321 atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc
     4381 ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt
     4441 cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt
     4501 cgtcatttct actattttta agctctttaa actattaggc tctcctagct cagcattaat
     4561 gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt
     4621 gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga atctcatttc
     4681 tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat
     4741 atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat
     4801 cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc
     4861 actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac
     4921 atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc
     4981 tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt
     5041 taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt
     5101 tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg
     5161 tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa
     5221 gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag
     5281 gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat
     5341 aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc
     5401 cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg
     5461 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     5521 gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt
     5581 taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact
     5641 aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg
     5701 gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt
     5761 agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctct
//
LOCUS       DACCXW010000145         5779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCXW010000145 DACCXW010000000
VERSION     DACCXW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.151"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..813
                     /locus_tag="IVY71_002540"
     CDS             <1..813
                     /locus_tag="IVY71_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7986562.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IVY71_002541"
     CDS             807..1229
                     /locus_tag="IVY71_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986563.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="IVY71_002542"
     CDS             2363..3331
                     /locus_tag="IVY71_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7986564.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="IVY71_002543"
     CDS             3341..3829
                     /locus_tag="IVY71_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7986565.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="IVY71_002544"
     CDS             4125..4406
                     /locus_tag="IVY71_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986566.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="IVY71_002545"
     CDS             4408..4824
                     /locus_tag="IVY71_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986567.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="IVY71_002546"
     CDS             5438..>5779
                     /locus_tag="IVY71_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7986568.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2154 a          982 c          947 g         1696 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcggttggt
     5281 acaggggcta cttggcatag tcgacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DACCXW010000146         5744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCXW010000146 DACCXW010000000
VERSION     DACCXW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.152"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(191..712)
                     /locus_tag="IVY71_002547"
     CDS             complement(191..712)
                     /locus_tag="IVY71_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7986569.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(715..1557)
                     /locus_tag="IVY71_002548"
     CDS             complement(715..1557)
                     /locus_tag="IVY71_002548"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7986570.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(1550..2275)
                     /locus_tag="IVY71_002549"
     CDS             complement(1550..2275)
                     /locus_tag="IVY71_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7986571.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            2451..2747
                     /locus_tag="IVY71_002550"
     CDS             2451..2747
                     /locus_tag="IVY71_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986572.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            3315..4907
                     /locus_tag="IVY71_002551"
     CDS             3315..4907
                     /locus_tag="IVY71_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7986573.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            4920..5660
                     /locus_tag="IVY71_002552"
     CDS             4920..5660
                     /locus_tag="IVY71_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7986574.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         1638 a         1127 c         1160 g         1819 t
ORIGIN      
        1 aaaagctgca cttccctttt ttatgtttct ccactaatcg aaatttagat agacatctag
       61 aaatcgcaat tccaaaaaat tctgtgtatt ggatacgcat cttttcttat tttttcttat
      121 ttcttccata tgggtgttta tctcagagat gatagaatag tttctaggat aaaaagtagc
      181 agctacgatg ttatccttcg tagaggattc ctgcttcagc tagtttttgt ttgatgtttt
      241 tcagcatatg cttttctggg tagcggatcg tatgcagatg gatgccgcct gttaattcgg
      301 ataacaaagt agcattactt ttttgataac gtctcataaa ggaatcgata tcttgtggcg
      361 ttttgatgtg caaagttcct gctaactcac cgtaaagggg atgttccaca attacatcaa
      421 tgatttcacc accattttca acgattagtc tcagttcttc ttctgtttga tcttttccat
      481 gctggacagc gattttactg atgtgcccag tattggtttc ttctgtttcg aggatataac
      541 ctcttgctgt tgccacgata cttttacctg cagctcgtag taaagcaata tcgcctacaa
      601 tgatctgacg gctgactcca aaagttttcg caaacttgct agcactcact ggtttttctg
      661 tatttgccaa ggtttgcatg atttgttttc gccgtgtctc tccttcgatc acacttattc
      721 ctccttcatt ttgttttggt attcgcgcga gtgtttcatc aaatcaacat acgtgtctgt
      781 tattgtttcc ttatatccct tatttttcag cagactaaca actttatcca atacttgctg
      841 ctctcttttt gcatcataaa ctgcttggtt ttcttctttt ttcacttgtc ctattttagt
      901 taccgtatcc attctttttt ctaataaagc aaccaactct cgatcgatca tatcgatctt
      961 atgacgtaat gtttcaagag actcttctgt tagttcttcc tctactgtct tgatgatacg
     1021 agcaggattt cctgcaatca ctacattgtc gggaaaagat ttggtcacaa ccgaacctgc
     1081 gccgactact acgttgtccc ctagagtgac acccggcgtg acgataactc ctccgcctaa
     1141 ccaaacatta ttaccgattt taatgggttt tgcgtactcc agaccgctgt tacgtttcac
     1201 tggatgtagt ggatgcgtcg ccgtgtaaag ctggacattc ggagcgaaca tgcagttatc
     1261 accgatttca atcgtgctga catctaaaaa tgtacaattg aaatttgcat aaaaattctc
     1321 cccaacaaat atattatatc cgtaatcaaa attgataact ggctccatat agattttttt
     1381 ccctgtacgg ccaaaagtct ctttcaacaa ttgggatcgc agttccgctg attctgcttg
     1441 gttgatgatt tggctttgac ttcttgcaaa catacggtcg gcaaccagct caggatcatt
     1501 ggcaaaataa agctcgcctg caatcatttt ttctttttcg gttttgaatt cagtcataat
     1561 ctgctcctaa ttcttgtatt tttttgaaaa caaccgctcc gtcttcttga ataaacgtat
     1621 aaggctccgc taaataaaca ggcgtttgat tgattactaa gattttggct gacggctggc
     1681 gaaactgtac cagatcacaa aacggatgaa cttgaaaact cgttccaaca atgacgatca
     1741 gctctgcttg gctgacggca taagccgcct gctccatgac tccatcttga aacccttcac
     1801 catataacac gatgtctggg cgtatttgtc ctccgcatgc ttgatgtttg tcgctgtgaa
     1861 gatattcttt ccagtctacc gtttgttgac attttcgaca atgacattga tacagactcc
     1921 catgaaaatt gacgagttgc gggcttttag cctttgcgtg caaaccgtcg atgttttggg
     1981 aaacaaccca aacatttttt tctttcgcta gatgtgctat ttccaaatga atgatattgg
     2041 gttttgcttc aggatgataa atctttttga caaattgata gaatttatcc ggttcattca
     2101 ccattgcttg atgactcagc aagtattccg gctgctccat cccgtggtag attcctgaca
     2161 aagatcgata gtctggcact ccagaaggtg tggatacccc tgcaccggtc aaaaatgtaa
     2221 tttgctgata agctaatatc tcttttgctg cttcgtctac tgttaattgt ttcatgtttt
     2281 gtcacctcac tttccattat agcccatact cgaacaaatt tattgttgaa aagtcagaaa
     2341 aatgggaata aaaaagtgtt atgggcattt gagcaattta tcacaatgtt cactgacaaa
     2401 agaatgaaaa cgtgttacaa tactgctgta taaaagtgag gtgaatcgat atgttaccat
     2461 tctttattta ttggggcgtt attctggctt gtatcgcctg gttagcgtta agcgtttatt
     2521 tctctgtttt ttatcttgta cgcaaagaaa atggaaatct ttgggctttt gctttattca
     2581 acgtgattgc cgcaattgtt ttagccatta cattagccgt atatcgaaca tggggctggg
     2641 gaatcaccca atactcaagt ttgatttatc taatattagc catttatggc gtcgttgttc
     2701 ttttgcaagc gatcctagga cgtgagccaa agaaagcggc agcttaaatg atcgagatgg
     2761 aactggacac aggaaatttt ctgttctagt tcttttttta ttgtattaca tacatttttg
     2821 tgtataatta tactatcgtt tgcaatggat agatgattca cattttgatt gttttttcgg
     2881 taaaagtaaa gatttctata tttatcagta ttttttcaaa aaccagcagt tatagattga
     2941 caaagcctct cgagtatcgt aaactttttt tgtataaata actgaatacg agatgcctca
     3001 atcaatgagg tagaggtcgc gagaatcatt aaccctgtgg agtgaagcaa acatggagaa
     3061 gcaggaatag ggaggatcgc cgaaatgtgc acttttttgc ttggagtgta tgttgggacg
     3121 caagttaaga gcttgcggac tgtcttagca gtgatgctaa gttgcgctat gttttgtgag
     3181 aggtttatca ccttatggaa ttttagcggc ctttttatat ctataaatcg gtcgcttttt
     3241 attttttcag ataaagcttt accgcatttc atctagaccg ctcgtgtatc atataatcag
     3301 aaagggagaa aaaaatgaaa aaaaatatgg tttcagttgc cgtactattt gcttttgtct
     3361 tctcactatt cagtgtggga tctctggcaa atgcagatga aaaaacaccg agcgatgagt
     3421 ttcgcgtagg tatggaggct gggtatgcac ccttcaactg gtctcaacaa acagatgaga
     3481 ataatgcctt gccaattcaa ggccaaaata gttacgccgg cggttatgat gttcaaatcg
     3541 ctaaaaaagt agcagatggt ttagggaaaa aattagtcat cgtccaaacg aaatgggatg
     3601 gtctggctcc tgcccttcaa tcaggcaaga tcgacgcgat catcgccgga atgagcccta
     3661 ctgctgaacg acgaaaagaa attgatttta ccgatcctta ttacgaatca caattagtag
     3721 tagtagttca aaaagatagc aaatatgcaa aagcaaaaag tttagcagat ctttctggag
     3781 caaagatcac tgctcagtta aatacattcc actatactgt gatcgatcag ataccagatg
     3841 tgcagaaaca acaagcaatg gataatttct cagcaatgag aacagcttta gcttctggaa
     3901 tgatcgatgg ttatgtcagc gaacgtccgg aaggtgtaac ggcaactagt gtgaacaaag
     3961 acttggaaat gcttgaattc agcggtgcag atggcttcca gacaaacccg gaagatgttc
     4021 agatttctgt tggtatgcgt aaaggcgacc cggatatcca aaaagtaaac cagattcttt
     4081 ctggcatctc taacgatgaa cgaaccaaaa ttatggacca agcagtcaaa gatcagcctg
     4141 ctgcaactga tagtgaagaa aaacctggca tcatttctga tttcactacg atttggaaac
     4201 aatacggcaa tatgttctta cgaggtgctg gacttacctt gttcatcgca ttgatcggga
     4261 cagttgtcgg tactacactt ggattactga ttggtgtctt ccgttcggta cctgaatcag
     4321 gaaatgcact gactcgcttc ttccaaaaaa tcggaaatgc acttctttct atctatgtcg
     4381 aagtattccg cggaacaccg atgatggtac aagcaatggt catcttctac ggtctggcac
     4441 tcgcttttgg catttcattg aaccgaacga ttgccgcttt gtttattgtc tctctcaata
     4501 ctggagctta catgacagag atcgttcgtg gggggatttt tgcagtagat gaagggcagt
     4561 tcgaagctgc gcaagccatt ggtatgaccc atggccaaac gatgagaaaa gttgttattc
     4621 cacaagtttt acggaatatc ttgccagcaa caggaaatga gtttgttatc aatatcaaag
     4681 atacagcagt tttaagtgta atcggcgtag ctgatttgtt cttccaagga aactctgctt
     4741 ccggtgcgaa tttccaattc ttccaaacat ttacgatcgt cgggatcatc tacttagtca
     4801 tgacctttac gatcaccaga atcctgcggg cggtagaaaa gaaaatcgat ggtccttcag
     4861 cttataccaa gcttgaagaa atcgacaatt caaatttgca ggaataagga ggaagaatca
     4921 tgaatgaaaa aattattgaa atcaaacatc taagaaaaag cttcggtgaa aatgaagtat
     4981 tgaaagatat cagcgtaaca gtcaataaag gtgaagttgt gacgatcatc ggttcttccg
     5041 gttctggtaa atctactttc ttgcgctgta tcaatttact ggaaaaacca acaggcggcg
     5101 agatcattta taatggagaa aatgtattag ctcctaaata taatttacct aaatatcgaa
     5161 cgaatctagg tatggttttc caatccttca acctgttcaa caatatgaat gtcttggaaa
     5221 actgtatgtc tggtcaaaca actgtcctga aacgagataa agaaactgcc aaaaaagtag
     5281 caatcgaaaa tctggaaaaa gttggtatgg atcgttatat cgaagccaaa ccagcacaat
     5341 tatctggtgg acagaaacag cgtgtcgcaa ttgctcgggc attatcaatg aatccagatg
     5401 tattgctatt cgacgaacct acttcggcgt tagaccctga aatggttggc gaagtgctta
     5461 atacgatcaa agacttagca catactggct tgacaatgct tatcgtgacg catgagatgg
     5521 aatttgcaaa agatgtatct gaccgtgtca tttttatgga taaaggtgtg attgcagaag
     5581 aaggtacacc agaagatatc tttgtccatc cgaaagaaga acgtacaaaa gaattccttt
     5641 cacgtatttt aaatgcttag gatcaatagc tgattgggac aaagagctgc ggtgtttacc
     5701 gcagttcttt tttatgaatt actcataaaa ggtaaatttc tgaa
//
LOCUS       DACCXW010000147         5679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCXW010000147 DACCXW010000000
VERSION     DACCXW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.153"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(240..606)
                     /gene="ssrA"
                     /locus_tag="IVY71_002553"
     tmRNA           complement(240..606)
                     /gene="ssrA"
                     /locus_tag="IVY71_002553"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(777..1550)
                     /locus_tag="IVY71_002554"
     CDS             complement(777..1550)
                     /locus_tag="IVY71_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7986575.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1694..2044
                     /locus_tag="IVY71_002555"
     CDS             1694..2044
                     /locus_tag="IVY71_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7986576.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2025..2741
                     /locus_tag="IVY71_002556"
     CDS             2025..2741
                     /locus_tag="IVY71_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7986577.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2741..4189
                     /locus_tag="IVY71_002557"
     CDS             2741..4189
                     /locus_tag="IVY71_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7986578.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4260..4769)
                     /locus_tag="IVY71_002558"
     CDS             complement(4260..4769)
                     /locus_tag="IVY71_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986579.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4759..5484)
                     /locus_tag="IVY71_002559"
     CDS             complement(4759..5484)
                     /locus_tag="IVY71_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986580.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1936 a          984 c         1120 g         1639 t
ORIGIN      
        1 gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt
       61 ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt
      121 tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa
      181 atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac
      241 aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt
      301 catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc
      361 gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa
      421 gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta
      481 ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt
      541 tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac
      601 gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat
      661 tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt
      721 tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa
      781 atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata
      841 aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa
      901 gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc
      961 agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact
     1021 gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag
     1081 tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct
     1141 aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg
     1201 ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac
     1261 tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct
     1321 tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct
     1381 tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg
     1441 ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct
     1501 agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact
     1561 ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg
     1621 aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt
     1681 taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct
     1741 ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc
     1801 cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct
     1861 tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta
     1921 ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga
     1981 aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga
     2041 ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg
     2101 aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac
     2161 attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa
     2221 gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg
     2281 aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca
     2341 gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag
     2401 caagagaaga agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga
     2461 tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta
     2521 aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc
     2581 agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc
     2641 atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt
     2701 ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca
     2761 actcttagct ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac
     2821 acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga
     2881 atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca
     2941 ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt
     3001 tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt
     3061 taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc
     3121 gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt
     3181 gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag
     3241 cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt
     3301 tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc
     3361 gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga
     3421 atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc
     3481 agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat
     3541 gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc
     3601 tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg
     3661 tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat
     3721 taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc
     3781 agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga
     3841 ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga
     3901 aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga
     3961 agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa
     4021 agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt
     4081 gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg
     4141 aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg
     4201 tataaaagat catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt
     4261 tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta
     4321 aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct
     4381 tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg
     4441 gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc
     4501 gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt
     4561 ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac
     4621 atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt
     4681 ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt
     4741 tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg
     4801 gataaaaaaa gtgaaaggga atagactaag aatgagacag catccactga gcccaataag
     4861 agtttgggca aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt
     4921 aaaaaaagtc tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg
     4981 gatatctggc agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc
     5041 ataaactgac tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg
     5101 aaagaacagc tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggtg
     5161 ccaattgatc aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag
     5221 gaaggcaaat agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc
     5281 taaagccgga agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac
     5341 ttgattggct aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc
     5401 agaaaagctt gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc
     5461 agtagcatag cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata
     5521 tgaataaagt atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta
     5581 aaaagcaaga ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa
     5641 cactgtttat tcgaaaagag gagcagcgac aataatttt
//
LOCUS       DACCXW010000148         5497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCXW010000148 DACCXW010000000
VERSION     DACCXW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.154"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..274)
                     /locus_tag="IVY71_002560"
     CDS             complement(<1..274)
                     /locus_tag="IVY71_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7986581.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(246..1199)
                     /locus_tag="IVY71_002561"
     CDS             complement(246..1199)
                     /locus_tag="IVY71_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7986582.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1811..3304
                     /locus_tag="IVY71_002562"
     CDS             1811..3304
                     /locus_tag="IVY71_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7986583.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3439..3735
                     /locus_tag="IVY71_002563"
     CDS             3439..3735
                     /locus_tag="IVY71_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7986584.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3836..4687
                     /locus_tag="IVY71_002564"
     CDS             3836..4687
                     /locus_tag="IVY71_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986585.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4708..4920
                     /locus_tag="IVY71_002565"
     CDS             4708..4920
                     /locus_tag="IVY71_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986586.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
     gene            5353..>5497
                     /locus_tag="IVY71_002566"
     CDS             5353..>5497
                     /locus_tag="IVY71_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7986587.1"
                     /translation="MNPFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIY"
BASE COUNT         1875 a          853 c         1043 g         1726 t
ORIGIN      
        1 aatcttttga tcgtctatta tatataatcc gttcctcgtc gtttaaagta tcaacatagg
       61 cgtccattgc aagtttgact atttcataca taggaatatc ttttgcataa ctaagatccc
      121 gaatggtttt tagtacggga ggatctactt gaactggttt tctttctttc gcagtgtact
      181 ttcgttcccc taactcgaca ttaatatcat ctttaattga aatatcttca gcaccaattt
      241 tcttcttatc tttgttcgtt ggtaataatg ccatttagtt taatctcctt tccaagaggt
      301 aggatatgat tggccattga gtcagcatat ttaggtgtat aatcaaatcc ttcaatatca
      361 ccatgttgtt catagtattc aattctttct tctaattctg taaagatgtc tgcaaaaata
      421 gcccaccatc ttcgatctac ataatcattt aattggatac cattctcacc atacatttct
      481 aaccgatccg agcctttaac aatgctttta aaaatgtttg attcaccata cttatcaatt
      541 gttttttgat agtttgcgtg ttgagaggaa cgtcgcactg agaaaagaac aggcaaaatt
      601 ccaatgattt gaagatgaga ctcttcaaaa ttcgtgacaa tcggaactaa aactttatta
      661 ataaaatctt ctgttccttc cattgcgtac cgttttaatt cttgcattgc aataatataa
      721 tctgcagcag ttaagaatgc acgaaccaca ttatctgttg atggtgggca gtcgataaag
      781 atataatcga aattttttct caattcttct aaaggtttaa tgaaggccaa tgatctctta
      841 cgacgatcag gatatttttc ggacaagtat tcattaagtt cttcagatcc aggagagcca
      901 gccataatat ataaatgtgg acttacttga gtaatacctg aaacccagtc cccttttttg
      961 attgctgcag acatggatcg atcaaagcta gtaatttgat acgttgatcc aatgttggaa
     1021 gtagtatttc tttgttggtc ataatcaatt agcaacacgt ctttttcaaa aagttgacta
     1081 gaaacaatcg ctaataaatc attgtctgta gtcttaccaa tgccgccttt tctacctgta
     1141 agcattacaa cttttcctag tttatctgat gttttaaatg cagtccgtaa gttttccata
     1201 gttccctcca atttcctata ctaaatatat ttagtatact tcatatactt aatatattcg
     1261 atatatctaa tatactccaa attgataaga attcagactt tataaggaat atagtggata
     1321 tactaaatat atttgctata ttcagtatat cagatatatt caaaatgtca aaatatattt
     1381 gtgcgttttg cgttgacaat ttagagtgaa aagaggtaaa cttaattcta tagaaaccac
     1441 agataattca ctatttacaa agtagcaaaa gcagtggtac tctttaaaca gaaaaattac
     1501 ataaaaaaaa gagacggaaa taattgattt tagcagaatc aattatttct gaatcacatg
     1561 gttaggacat gtgttcacgc tcgtctcaag aatatgtaag tagctaccaa ctacttatat
     1621 taaaagttga tcggatacct tttccgattg cctttattct accaaaaaaa ggcgagcaac
     1681 tcaacagaag aacgtttatt tagtgtactt aaattaaata ttcttaaaca ctgaagagag
     1741 aacacatact atggctgaga ttaaagctaa actgtgttct ctttttgttt gagaaaggac
     1801 gttacaatcg atgttgagcg aaatttattc agctattctt aaaaacggct tacgtacata
     1861 caaatatcga aacagcacca tgaaacctgc aggtcatatt gattccagta ggagtggtgc
     1921 tatctttgcg taccgtagca agcaattaat gaatgttggg cgtggtatgg tcattacttc
     1981 ggaagaagct attttagaga acgaaaagaa actaacgcat tggacaccta atacctatcg
     2041 ctttggtact tatgcggatg attatcgaac agtggttaaa ggtcattctg agaaaaactt
     2101 aagtcaaatc aacacatttg tagttgatat cgattcaaaa gagaaccatc aaggcgaaat
     2161 tattttagcc tgtttagatc aagtgggcta catgccaacg ctaattttag aatcagatca
     2221 tggttatcaa gtttattttg tactaaagac accaggctat gtcacaaaga aaagcaattt
     2281 caaggtgatc gatgtagcaa aaaaaatttc aaaaacaatt cgtctacagt tggctgaaaa
     2341 attgtccgga gtggatctag gatgtaacca cttcggaatt gctcgattcc cacaagaaaa
     2401 taatattgtt tttaaagaaa tgaactatca gtattctttt gctgactgga ttcaatggtc
     2461 gatgaagctt gaatcagatc aaaaaacaaa agaagatcga gaacgaaaac taattgtctt
     2521 tcctgagaaa aaagaaattc gtcaagtaga tgaaccgtgg ttcgatatgt tacttcataa
     2581 agcaaacatc gtcggcggag aaggacgtct tggtcgaaat aatgtgattt tcacgctttc
     2641 tttagcttat tactcttcag gttatgcatt ggatacttgc gaatacaata tgttcacatt
     2701 caacgaacgg ttacaggaac cgctatctga agcagaatta gtaaagattg tacgtagtgc
     2761 ttattctggt aattatcaag cagcaagcag agaattcatt attgaattgt gccagcaatg
     2821 ggtttcacca accttaaaaa ataatgatct gttcattcaa cgacgtggct ggtggaaatt
     2881 taaaaagcca cgagatcaaa gaaaatatag tcataaaaat gaatggaaag aagatctatt
     2941 ggcttatcta agcgaaaaaa gtcttgctag tgagccttat ttgaacctgt caaaaaaaga
     3001 attggcttca caactaacga tgcccaagcg aacacttgat aagatcttaa atgagttacg
     3061 agaggagaat aaagtcttct acaaggtaac acgaggaaga aatggcggaa tgatcattgc
     3121 ttcagtcaaa acgttgttcg cgagggttat tcaactaagt aaagaaaaga gactagatta
     3181 ttcaacaagg atcacagagg tatttggact caacgagagc ttagtagaag agacatttag
     3241 acaattatca acccccttga accctcttaa agaagtgaat ctttttgaag tagatacagg
     3301 ttgaaaaata aaaggtgcaa attgccttat acaatatatc tatgatactt ctgtaggtgt
     3361 tgctcccttt gcctagctac gtgtaggtgg tatacttata actgcgtatg cagtcttatg
     3421 tatcatggag gaagttgtat gtcgttaaaa aattatgtct attcacatcc tgttaatgtg
     3481 tttatcattg gtcgtttggg attatctgtg gaagtgttct gtgagcttta tggttttaag
     3541 caggggactt tgtcttcttg ggttaccagg gaaaaaacag ttgcttcttt gccgattgag
     3601 tttcttcatg ggttgtcctt agcttctggg gagactatgg atcaggtata tgattgtttg
     3661 tgtgtgttag agcaggagta tattgagtat aaaatagcta acgagttaag aaaaaggaag
     3721 aagtatatcc agtagtagtt tttatttact taaaaaattg gttcattgag caacctatcg
     3781 ctgctaaagt agcaaaggaa aacgttaaag gaaagaattt agggaagaaa tcgaaatgaa
     3841 atcggacgac tggattgaac acacctggct tcctctgaaa agaaatagca aacgaaagaa
     3901 attttgggta ttgcaattat ggccattagg aaaactttgt agttacgggg cacgacttat
     3961 tcttgattta accgtttact cgattggagt atttggaacg gcttattact tgataaatga
     4021 tcccaatagg attgcagcat cagacatttt tacagtgcta atgacgttga tttttgtcat
     4081 gctattgtct atttttactc aattagtcat gcaatttacc aggcaaaaat taacagcagg
     4141 aaaacttttc aaaagcctat tcgatttaat tgttgtaaat gcaattctag cgactttaat
     4201 aaaacaaatt gaacaatctg gaagaacaac aaaaggagtg aaggattttt tattgacgtt
     4261 tattcacttt gtaaatggac acatgtattt gtttgaagtg ggtattattc tcttttttgt
     4321 cgggtatgta tccacgaaac ttattaaacg ttcaattgaa aaagaaattg aaattgacct
     4381 gattccgaca cttcttggtt atgagtacaa tgttgatttt ggaagatatg acgaattgat
     4441 caatacggat atcgttcaca cgagtatcca tgaatctact gttaagggca tcagatctat
     4501 tttaaaaaat ggccaggaaa tgaaagcaat agatcgaaga aaagagatct acaattttta
     4561 tgaagtcgaa gcaagcctac gatatggcat cggaaaattg tggttagatg gaagtaatat
     4621 tcgtcaaaac aaaccataca ttttttggaa cagtagtgat ttagaacttc caatgaaaga
     4681 taaataatta tggagaatcg tttgaaaatg attcctctat acaggattgt gcttatagtt
     4741 ggcatggagt taaaaacctt aaagaaggag cagttattca aagtgaaaga acgtcataat
     4801 cagttactat gtagtaaaac gggtacgatg aacattgaat accgaacaga atatttagaa
     4861 ttaacgaatc aaactgattt tttgttgatt cgaaagcaac aagagcaaga aaaaaattaa
     4921 tggctactta tagccaattg attgatgaat gggaacaatt caaggaatct aaaattccag
     4981 aacgtctcta aaagccttta tttgtaaaga gcataaggaa acgtgggaac actttttaaa
     5041 aaaagtgttc caataggctg tgcctattac gtttctcaaa aataaatgtg ggaacaaaat
     5101 atttaaaaat aggatagtga aaggatagtg aaggatagta aaaggagggt agtaggatag
     5161 caaaaaggat aggctccttt tcgctatcct ttttaaattg gaggaattaa tatggaagta
     5221 agaataagaa atgtggatga gaaaatcgta agagaaattg atcgaagatg tgacgaacgg
     5281 ttctgttgca aagttttaaa tctactatca aataaggtag aataatagaa aaagatagca
     5341 ggaggaatga cgatgaatcc ttttaaagga aagcaatttc agcaggatgt gattattgta
     5401 gccgtgggct actatcttcg ttataacctt agctatcgtg aagttcaaga aatcttatat
     5461 gatcgtggca ttaacgtttc tcatacgacg atttatc
//
LOCUS       DACCXW010000149         5386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCXW010000149 DACCXW010000000
VERSION     DACCXW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.155"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(51..1598)
                     /locus_tag="IVY71_002567"
     CDS             complement(51..1598)
                     /locus_tag="IVY71_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7986588.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IVY71_002568"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IVY71_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7986589.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IVY71_002569"
     CDS             complement(2043..2237)
                     /locus_tag="IVY71_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986590.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(2463..3665)
                     /locus_tag="IVY71_002570"
     CDS             complement(2463..3665)
                     /locus_tag="IVY71_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986591.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(3668..3862)
                     /locus_tag="IVY71_002571"
     CDS             complement(3668..3862)
                     /locus_tag="IVY71_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7986592.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(3909..4757)
                     /locus_tag="IVY71_002572"
     CDS             complement(3909..4757)
                     /locus_tag="IVY71_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP7986593.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(4962..5312)
                     /locus_tag="IVY71_002573"
     CDS             complement(4962..5312)
                     /locus_tag="IVY71_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986594.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
BASE COUNT         1476 a         1054 c          960 g         1896 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttgaaaaacg ttgatttatc
     2341 ggcattcccc atccatatta tatttacgtc ttattcccac tcaatatgag tagcctcgta
     2401 aagaagctat tatatcagtg ctttttcagc ctattcctca ttttggtagg gaaatggtag
     2461 ggttagattt ttactgcttg atttccatta aagtttgcaa ctttagattt tgttgcacag
     2521 gtaaatttaa tgtttcctgt catttgttct gcaattcctt catcattacg gctccataga
     2581 tgagcgtagt gctttaaagt gatttcaggg ctagaatggc ctaaccgttg cgacacgacc
     2641 agaatgtctg cgttaaactc attgattagg tagctaacat gagaatgacg taatcctttt
     2701 ccttgtatta ggggtacctt agctagtttg gcataacgct gaataattcg ttgaacagtt
     2761 gaacgataga gtgggaggtc agtgtagcta agaataaatt gttctacacc atgatttttt
     2821 tgaataactt tccacttttt tagcacttca attgtatcat cgtccagaga aatgatccgc
     2881 aagccgcttt cagtttttgt gtaaggtttc cgaataaaat ctttttgatt tgtcatttcc
     2941 aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca cgtcattcca attaagtgct
     3001 aatccttcgc ttacacgaac accagtcatg taatatagcc agatcattaa aaaacacata
     3061 tgttcgtaga aatcatcttt ataaattttt gccaaaactt cttcaaattc ttcttttgtc
     3121 cagtaaggaa cgattgattt tccttttgga attgcttttg ttcttttgga aatattctca
     3181 ttcagaaatt gcagggtgac cgcatagtct aatgatttac gaaacatacc gtacataagt
     3241 gaactgtatg cttgtgagta tccgctatcg ttcaataacc aaattcgata attttcacaa
     3301 tctcgcacat tgatttcacg aagcttctta tcaccgaaaa attcagtaat ctgaatcaat
     3361 ccacactcac gagaactcca agtacttttt tgtacgctag ctttgtagtg tggaacgtaa
     3421 gactgctcca taaattcttt gtaagttaaa tcataattcg caaatccatt tacaaccatg
     3481 tattcatttt ttatacgcat gacttctttg tgtgcttcac gagctgatga gaatcgtttt
     3541 cctagagaat cttttgttcc tttcttctgg atcctctttc cagtcacttg atctgttcct
     3601 aactcaacta gataagaaaa gcgaccattt ttatcttgat acacacaagg gtattttgtt
     3661 ttcatagtta ttcctcctgt gaaagaggaa cacctataat ttcttcaatt acttgtcggg
     3721 gtactacccc aactctttta ttattataat atggatatcc ctgatttacc attcttgttt
     3781 ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa tcctagagcc tctaaatctt
     3841 ttcgacaaat tgtatttttc attgtataaa ctcactttca aagaaaacac tatcattggg
     3901 taatagtgtt atttctttat ttttgtataa tgaacggtac tttcttgaaa atccatcact
     3961 tgttcaggac ttcccaactc atttaaagct aaagcattgt aaaaaaatgg acgtctaact
     4021 atatattcgc ttgtatatag tatatcttga ttcgtggaat catagggttt ctttaccatc
     4081 tttttggtga cagttggtag tttgagattc cgggacttga aaaaagcttt tttcttatgc
     4141 tctttcaatt ccagctcttt atcgaaatat ttgctgatat agcgcccgcg attttcgata
     4201 ctgtctacat cgatcttgtt gatcttaacg aaaccctgac cccaaatcgt agttaaccgg
     4261 ttaaatgaga taaaggggaa atcaaacaag ataatatggt agtggattgc accgcgttgc
     4321 tgcttttccc acgtggcgag atactttaaa tttgcttttt tagttttata gaggtcataa
     4381 ttcagtcgtt tgataaattt tttaaattca ttatttgcat actcaatgtc ttgaatgttt
     4441 tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa aattcgtgtc tacaaggcgg
     4501 gcaatctcaa atcgcatact ttgataatgt ttctgtttgc gttttaaact gtcatattgt
     4561 ccttgagcgg ataattcatc atagtttttt cttttttcaa gagtaatatc attttcataa
     4621 atccaagcta atttttgttt tcgttccaga tttacttcgt tttcgatcgt tttacgtttg
     4681 ggagtaatga ttgcttcata atcataaatt tcaatatacg tgggagtttc aatgattttc
     4741 tgattataag cttgcaaaaa tcatcactcc ttgttgcgta ttcgattgtc aaagaaccag
     4801 tcattttctc ctgatgttga tctataaatc aagttaaggg ggaaggcctg cgtagcagcc
     4861 cttctagcct atggggtgtt gggcggtctt aacggacaga aacggctctg tgggcgctgc
     4921 cccgccgttt cgtcctaacc acccaacact ccatagtata gttagttttg tgtagtggta
     4981 ccttgttttc tgggaatcaa agttgccgta tcagcggttg ccgataaccc ataactgaca
     5041 aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat tggtaaatgt aattatggct
     5101 aaaccttgtt gggtaagttc ttccggcgtg atcacaggat gttcagattt gactttgaca
     5161 ctgatttttt cgtagcgaag ttttggtagg atacattcgt agctccaacc tagaatttcg
     5221 ccctcctttt gccagcgagt gacattgacg acttggtatt ctttttcgag tgtttctttt
     5281 ggtaaactga gatcggtaat ttttattggc atcacaattc tcctttttta tacgaaagtt
     5341 tatacgattt gattaaaaaa tatagatggc catctacgac ttgtat
//
LOCUS       DACCXW010000150         5377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCXW010000150 DACCXW010000000
VERSION     DACCXW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.156"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            38..829
                     /locus_tag="IVY71_002574"
     CDS             38..829
                     /locus_tag="IVY71_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7986595.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            795..1748
                     /locus_tag="IVY71_002575"
     CDS             795..1748
                     /locus_tag="IVY71_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP7986596.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2146..3120)
                     /locus_tag="IVY71_002576"
     CDS             complement(2146..3120)
                     /locus_tag="IVY71_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7986597.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3235..3669
                     /locus_tag="IVY71_002577"
     CDS             3235..3669
                     /locus_tag="IVY71_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7986598.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3794..4780)
                     /locus_tag="IVY71_002578"
     CDS             complement(3794..4780)
                     /locus_tag="IVY71_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP7986599.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5208..>5377
                     /locus_tag="IVY71_002579"
     CDS             5208..>5377
                     /locus_tag="IVY71_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP7986600.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGI"
BASE COUNT         1703 a         1025 c          960 g         1689 t
ORIGIN      
        1 aacaaaaatt gacttttatg ttaaaggagt aattcatatg ctgaaaaaag aaagacaaga
       61 acaaatcatt acattattag agaaagattt ttatttaact aattcagaaa tcgctaacaa
      121 attaggcgta tcagaaatga ccatacgccg agatgtcact gaattagcaa aaaaaaataa
      181 agtaaaaaag ctatatggtg gcgttgaacg aaaggaacct tataatcgag aactatcgac
      241 tcaagaaaag attgatacga atgtagccga aaaaaaatat attggtcaag tcatgaacag
      301 tatgattccc gataattcta ctatttatgt cggtgctgga acaacgatcc tatatgcact
      361 tcctttcatt caaaagaaaa atctatttgt aattacaaac agtttgattg ctttcaacta
      421 tttaaagaat catacaagtt atcggatttt acttactggt ggcgaatttt ctcctattac
      481 agaagagttc attggggaag ttgcagaaag atcttttgaa agtttgaatg tcgatatctc
      541 tttcgctgca acaaacggca tatttgataa caatgtgaca accgctcaat ttattgaggg
      601 aaatattcag aacgcagcgc tcaatgcagc aaaaatcaaa tgcattgttg cagattattc
      661 aaaattaaat aaatcagacg tctatacttt tagaaaatta accgattttg attatctaat
      721 aaccgataat catttatcag aacaggattt caatcattat tcaaaatact taacaatatt
      781 aaaggagccc aaaaatgata ctgacaatca cgatgaaccc atccattgat tccatttatt
      841 ttacaaaaca ctttgaatta ggtacgatga cacgctttga ttctccggta cgctatgtag
      901 gcggcaaggg aatcaattgc ggacgagctt ctgctctact aggttcatca gttattttaa
      961 cagggttttt gggcggttat tttggcagta tgattcaaga tatgctgaaa aatgaaaatt
     1021 tgtttgattt gcatttttta cctattgcag aagaatcaag aggtgccgtc acgatcatgc
     1081 atgatgatga aacgcagaca gagctagtgg aagctggtcc tgaaatagca gatactacta
     1141 tcaaaaaatt aatgaatgat atcaaactaa tcagtaaaaa taattacctt tcagtcatta
     1201 caataaatgg ttctgttaac aatccagatc cctttttgta tgctaaatta ttacaatata
     1261 tcaaagaaca aatagatcct tccacctata tattaatgga tatatctgga caacatctcc
     1321 gtgaagtctt agcaagtaag tataaaccga tgttcatcaa acctaatatc catgagtttt
     1381 cagatatcat ccatcaaaaa attactcaaa aaagcgacat aaaacaccat ctaaaaacaa
     1441 atccactatt taaagatatc gatgttatca tgatttcttg cggctcgtat ggtgcagtag
     1501 tcaaagtaaa agaagaattt tggaatgttg agatccctaa gattcctatc attaatacaa
     1561 caggctctgg ggatgccact gttggaggtt ttgctcattc attagaacag aattatcctt
     1621 taaaagaatg tgtaaggtac gcaatggctt gcggcatgtc gaatgcacag caatcagaag
     1681 tcgggaaagt tattccagct gatgtaaaaa atttagcaaa aagcattaaa atcacagaaa
     1741 tgaaataaat agtacaaagt aaataattgt ttatatgaaa tgagacacaa agagactgtg
     1801 acaaaaaacg tgtgtaccga cccccaaaag ttagattttt tggtctaact tttgggagtc
     1861 gatacattgt cacagtcctt ttttcgaata aacggtgttc tcctgccaac tcctcggtca
     1921 caagtcacaa tattcaacaa acatgagaag ctattttttg aaattgctcc ttgtgactca
     1981 gagttaaacg gcaggctcac gacctcttgg tataaacggt gttcaaaagc ttgcatctac
     2041 gctaatgagt ccaaccaagt caaaaatgag gagctttttt ggctgtcctt attacttgat
     2101 gcagggcagt tttttcataa tctcttttag attaaatcct tttggttaca caaggttctc
     2161 ttcaaagaat gttgccattt tttcaaatgg aatgatgtct gtctggtcgt atagatctgt
     2221 atgggatgct cctggaatga tcatgagttc cttgttgtca ccttttagtt ctttgaaagc
     2281 gtcctcccca aagtaacgtg aatgagcttt ttccccgtga atgactaaga ctgcatgacg
     2341 gatttctgga atataatgca atagttttgc attcataagt gacaaccctg atgtcctatt
     2401 ccaaccatca ttggagttta aagaacggac atgatatccg cggtctgttt tataatacgc
     2461 atgatagtct ttgacaaatt gaggagcatc ttcaggcaac ggatcaacca cacctccagc
     2521 acgtgcgtag tccccttgtt tgaaatcaat gatgcgttgt gcatttaatt cttcacgcat
     2581 ttcatatcgg gcattttcat ccataacatc gaagtagcca tttgctgtta ctcgagtcat
     2641 gtcatacatt gtggaaacaa ctgttgcttt gacccgcgta tcgatagctg ctgcattcaa
     2701 tgccatacct ccccatccac aaataccaat gattccgatt ctctcagggt ttacattttt
     2761 ttgtacagat aaaaagtcta ctgccgcttg gaaatcttcc gtattgatat caggcgaagc
     2821 aacatatcga ggtactcctc cgctttctcc tgtaaacgat ggatcaaacg caatcgttaa
     2881 aaagcctctt tcagccatcg tctgcgcata tagaccagac gattgttcct tcactgcacc
     2941 aaatggcccg ctcacagcga ttgccggcaa tttcttttca cttacttctt ttggttcata
     3001 taaatcagcg actaatgtga taccaaaacg attcgggaag ctcacctttc gatgattgac
     3061 ttgattgctt tttggaaata ctttgtccca ttcttgtgtg atgtttaatt ctttattcat
     3121 attctagact ctcctttgat caaaattgac atttgttttt gaatgtatta ttatgataaa
     3181 acttaaactt gactttaggt caaggtaaaa attttatttt aggaggattt ttttatgtat
     3241 tccattggac aagtgtctga aatgtttgga ttgcctgttt ctactttacg ttattacgac
     3301 aaagagggat tatttcctga tatccagcgt gtttctggta ttagaaaatt ttcagagaat
     3361 gagttagagg ctttaagagt cattgactgt ttgaaaaaat ctggtttaga aataaaagac
     3421 attaagcaat tcatggaatg gagcaaagaa ggcagtaaga catttgaggt tcgtaaagaa
     3481 ctatttgaaa aacaaaaaga agtagtagaa aaagaaatag caaaattaga aagagtactc
     3541 gctatgctaa actacaaaag ctggtactat gaagaagcaa taaaagccgg caatgaagaa
     3601 gccgtattga caatgattcc tgatgattta ccccaacatg taaaagaagc ttatgtacat
     3661 tctcattgaa caaacggata actcactctc cttctctatg aacttcatga aaacatgaat
     3721 aaacacctct aactgattgt tagaagtgtt tattttttca ctcaagtatc ctgcacttac
     3781 aaaaatatcc tttttaccgc catcctaatt ctggagcgat atcttttacg attgtttcaa
     3841 gcaagtgcat gttatattct acccccaaag tattaggaat cgtgacaagt actgtgtctg
     3901 cttctttgat tgcttcatct tcagctagct ccttgattaa ttgatcaggt tcaccagcaa
     3961 aagtttttcc aaaaatcgtt gggtttcggt gataagcaag atagccgaca gcatcttgtt
     4021 gcggactacc gtcttgtcca aataagcgtc gatccaaatc agtggtgatt ggctgaatcg
     4081 agcgagtaac taatgttctt ggttcaaaat catgacctgc ttgcttccac gcttctttat
     4141 acgcacgtaa ttggtttgct tgttggatat ggaatggctc gtgggattcg taattgacta
     4201 atgtagatga ttgcagattc attccatgtt gtgctgccca gattgccgtt tgctgcgaac
     4261 cagctcccca ccagattctt tgtcgtaatc cttctgaata aggttctatc cgtaattttc
     4321 ctgatccttg tgggaacatt ggttgaggat ttggttcttc aaatggtttt ccttcaagta
     4381 attccaagaa ttcaagggtg cgttcccgtg ttacctcttt gatttcttct tcactgtaat
     4441 cataaccaaa gtatttccag ccatctagaa cttgttcagg tgaaccacgc cccactccta
     4501 gttgcactct tccttgcgtg atgatatctg tcaatccggc attttctgcc atataataag
     4561 ggttttcata tcgcatatca ataagtcctg taccgatttc aatcttactt gtctttgcgc
     4621 cgattgccgc tagcaaagga aacggcgacc caatctgagg ggcaaaatga tgtacgcgga
     4681 aaaacgctcc gtccactccg attttttctg cttccaccgc taaatcaatc gattgcaaat
     4741 aagcatcttg agctgatttc acaagcgatc cttgctccat ccaatgtcca aagcttaaaa
     4801 agccgatttt tttcaaggta atgacctcct ttaaacctac aaaaatggaa ggttttttat
     4861 ttttaatatg aactgattct actacttttt ttcttaaaag acgaataatt gttctcgcct
     4921 cacaatcaag aaatgtatcg gcttactaca cccctacttt cttcatcatc tgccgtgtct
     4981 taatctcttt taacagtctg ttacatcgtt gatttttgac tcaaaatgat tgtttctaac
     5041 atgtatttat ggtaaaaaag acaagattag ttctttgtac acatcgattt atacgctgaa
     5101 caaaaagaaa tatataaact aagaaaccca ttgttttttc gctccatcac atagagttta
     5161 ctttagataa acagtgacat ttactaatcc ataacggagg aaatcaaatg aaaaaaatct
     5221 atttcttatg tacgggcaat tcatgccgca gtcaaatagc agaaggctac gcgcgtaaat
     5281 acttgtctcc ttcaatgttt gagataagaa gtgcggggat cgagacacat ggcttgaatc
     5341 caagagctat aaaaatcatg gcagaagacg gcatcga
//
LOCUS       DACCXW010000151         5338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCXW010000151 DACCXW010000000
VERSION     DACCXW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.157"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(218..400)
                     /locus_tag="IVY71_002580"
                     /pseudo
     CDS             complement(218..400)
                     /locus_tag="IVY71_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            417..584
                     /locus_tag="IVY71_002581"
     CDS             417..584
                     /locus_tag="IVY71_002581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986601.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(627..1202)
                     /locus_tag="IVY71_002582"
     CDS             complement(627..1202)
                     /locus_tag="IVY71_002582"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7986602.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1357..1883)
                     /locus_tag="IVY71_002583"
                     /pseudo
     CDS             complement(1357..1883)
                     /locus_tag="IVY71_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            2340..3851
                     /gene="gadC"
                     /locus_tag="IVY71_002584"
     CDS             2340..3851
                     /gene="gadC"
                     /locus_tag="IVY71_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP7986603.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3864..5264
                     /locus_tag="IVY71_002585"
     CDS             3864..5264
                     /locus_tag="IVY71_002585"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP7986604.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
BASE COUNT         1637 a          922 c          973 g         1806 t
ORIGIN      
        1 attgaatcaa ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt
       61 ttttacagtg aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt
      121 aatagctaat gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat
      181 tttgtgtaaa taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta
      241 atgtatccgt cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg
      301 tatcaaagat ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc
      361 tgcggctgta tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg
      421 agaacacttt tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc
      481 caactgattt aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt
      541 atagatacct aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc
      601 aattatttga ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc
      661 gaaaggtaat cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca
      721 tcaacaatac gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt
      781 tcctcaattt tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga
      841 aaatcattct tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat
      901 agatcaataa tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact
      961 tttccgtact gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt
     1021 gcaatagctg gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta
     1081 tctattgcgc ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact
     1141 ttcagtagct caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc
     1201 attaaattta ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc
     1261 agctgctcgt ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata
     1321 agccattaga gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc
     1381 tgaaaaactg aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta
     1441 aatgcattta cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag
     1501 tgatgcccat acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg
     1561 aagaggtgaa tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc
     1621 tgttgcttga actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg
     1681 gtcttgaccg tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga
     1741 tcgtactttt cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct
     1801 ttttccaaat ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg
     1861 atttctgtag taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac
     1921 tctattctac agaagggact tccttttttc tatttacaca aaatatttta cactctcaac
     1981 aaaagtatct tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg
     2041 aggtactttc tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat
     2101 acgatataaa acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg
     2161 ttgtcagttt tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat
     2221 tttgcttgct atactttgtc ttgtagaaaa gaggtagaca aaataatgca agctcaaata
     2281 agcgattttc cataaccttg gtaggaatga caaattaaat aggaaaggga agtaataata
     2341 tgaataaaaa aaagttatct ctattcggtt tttttgccct gacagcttcc atggttttga
     2401 ctgtttatga gtatccgacc tttgcaacat cacaattaca tttggttttc tttcttcttt
     2461 tgggaggtat tttatggttt ctaccagttg ctttatgtgc tgcagaaatg gcaacagttg
     2521 aaggatggaa agatggtggg attttcagtt gggttagtca aactttagga gaacgttttg
     2581 gttttgcagc tatttttttc caatggtttc aaattacagt aggttttatt acaatgatct
     2641 acttcatact aggagcgctg tcgtatgtaa ttaattttcc agagcttaat acagatccat
     2701 ttatcaagtt tattggtgta ttagtcattt tctgggtact gactttttca cagttgggcg
     2761 ggaccaatcg taccgctaaa attgctaaag caggatttgt cattggaatt gttattcctt
     2821 cgatcattct ctttggttta gcagcagcct actttatcgg cggtaatccg attcaaatgg
     2881 ctattaatgc gaaagctttt attcctgact tttctcagct ttcaacttta gtagtatttg
     2941 tatcttttat attggcttat atgggcgtag aagcttcagc atcacatatt aatgagctgg
     3001 aaaatcccca aagaaactac ccattggcaa tgtttttact agtgattttg gccatcgcct
     3061 tagatgcaat cggcggtttc tcagtagcag cggttattcc acaacatgac ttgtctctca
     3121 gtgctggtgt aatccaaaca tttgaaacgc ttattttaca ttttaacagt catttacatt
     3181 ggttggtaaa agtgattgct ctcatgattg cctgtggcgt tatgggtgaa gttagttctt
     3241 gggtagttgg tccatcacga gggatgtttg cagctgctca gcgaggtctg ttaccagttt
     3301 tcttccggaa aactaataaa caagacgttc cagttccgtt gattatgata caaggaatta
     3361 tcgttactat ttgggcagca gtcttaactt ttggtggagg aggcaacaat ttatcattct
     3421 tagtagcaat ctccttaact gttgtcattt atctcgtagg atatttacta tttttcctag
     3481 gttactttgt attagttttt aagaaaaaag acttaaaacg aacttacaat gtaccaggaa
     3541 aaactgttgg caaaacgatt atcggtggaa ttggttttat tttatcagtg ttcgcattgc
     3601 ttatctcttt tgtaccacca gcttcaattg cagctagtga gcaccgaaca taccaaatca
     3661 tactacttat tagctttgta gtgactgttc ttttaccttt tatcatttat gaagtacatg
     3721 ataaaagtgc tcacacaact attgaggaac ctactcacgt gaaagctgaa gacgttaatc
     3781 ccgctgttta tccagctgct agaggagagc accacgttac aaaaaatgat gaacatattt
     3841 taaaacatta ggaggaattc attatgttat acggaaaaga taatcaagaa gaaaaaaact
     3901 atttggaacc aatttttggc tctgcaagtg aggatgttga cttgcctaaa tataagttaa
     3961 acaaagaatc cattgaacca cgaattgctt atcaattagt acaagacgag atgttggatg
     4021 aaggaaatgc gcgattaaac ttagctactt tttgtcaaac gtatatggaa cctgaagcag
     4081 tgaaattgat gacccaaacg ttagaaaaaa atgcaattga taaatcagaa tacccacgaa
     4141 caacggaaat tgaaaaccgc tgtgtaaata tgattgctga tttatggcat gctccaaata
     4201 atgaaaaatt catgggaact tcaacgatcg gctcttcaga agcctgcatg ctgggtggta
     4261 tggccatgaa atttgcttgg cgtaaacgtg ctgaaaaatt aggtcttgat attcaagcaa
     4321 aaaaacctaa cctggtgatc tcttctggtt accaagtttg ttgggaaaaa ttctgtgtat
     4381 attgggatgt ggaactgaga gaagtcccaa tggatgaaaa acatatgtca attaatctag
     4441 atactgtcat ggattatgtg gatgagtaca caattggtat tgtaggtatt atgggtatta
     4501 cttacactgg tcgttatgat gatatcaagg gtctgaatga tttagttgaa gctcacaata
     4561 aacaaactga ctataaagta tacattcatg ttgacgctgc atcgggtggc ttttatgcac
     4621 catttactga acctgatcta gtttgggatt ttcaattgaa aaatgttatc tcaattaatt
     4681 cttcaggtca caaatatggt ttggtatatc caggtgtggg ttgggtctta tggcgtgacc
     4741 aacaatactt accagaagaa ttagtattta aagttagtta cttaggtgga gaaatgccaa
     4801 ctatggctat caacttctct catagtgcag cacaactaat tgggcaatac tacaactttg
     4861 tacgctatgg ctttgatggt tatcgtgata ttcaccaaag aactcatgat gttgctgttt
     4921 atttagccaa agagatcgaa aaaactggta tttttgaaat cattaatgat ggatcagaat
     4981 tgccagttgt gtgctataag ctgaaagaag atcccaatcg cgaatggaca ctatatgatt
     5041 tatctgatcg tctgttaatg aagggatggc aagtcccagc ctacccatta cctaaagact
     5101 tggatcaatt aattattcaa cgcttagttg ttcgagcaga ctttggaatg aacatggctg
     5161 gtgattatgt acaagatatg aaccaagcaa ttgaagagtt gaataaagct catattgttt
     5221 atcataaaaa acaggatgtg aaaacgtacg gctttactca ctaaaattct tcagagaaat
     5281 ataaaagaag ttaattaact aataaaagga gttgtgatta ccacacagct ccttaaat
//
LOCUS       DACCXW010000152         5303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCXW010000152 DACCXW010000000
VERSION     DACCXW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.158"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            32..388
                     /locus_tag="IVY71_002586"
     CDS             32..388
                     /locus_tag="IVY71_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986605.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="IVY71_002587"
     CDS             404..754
                     /locus_tag="IVY71_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP7986606.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="IVY71_002588"
     CDS             783..1562
                     /locus_tag="IVY71_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP7986607.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="IVY71_002589"
     CDS             1573..2235
                     /locus_tag="IVY71_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP7986608.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="IVY71_002590"
     CDS             2254..3360
                     /locus_tag="IVY71_002590"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP7986609.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="IVY71_002591"
     CDS             3350..4441
                     /locus_tag="IVY71_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP7986610.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="IVY71_002592"
     CDS             4454..5194
                     /locus_tag="IVY71_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP7986611.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1704 a          867 c         1262 g         1470 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agc
//
LOCUS       DACCXW010000153         5161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCXW010000153 DACCXW010000000
VERSION     DACCXW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.159"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(587..877)
                     /locus_tag="IVY71_002593"
     CDS             complement(587..877)
                     /locus_tag="IVY71_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986612.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1219..1515
                     /locus_tag="IVY71_002594"
     CDS             1219..1515
                     /locus_tag="IVY71_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986613.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1522..3474
                     /locus_tag="IVY71_002595"
     CDS             1522..3474
                     /locus_tag="IVY71_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7986614.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3546..4043)
                     /gene="dfrG"
                     /locus_tag="IVY71_002596"
     CDS             complement(3546..4043)
                     /gene="dfrG"
                     /locus_tag="IVY71_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7986615.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4370..5062)
                     /locus_tag="IVY71_002597"
     CDS             complement(4370..5062)
                     /locus_tag="IVY71_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7986616.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1666 a          899 c          859 g         1737 t
ORIGIN      
        1 cgatagtcaa caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta
       61 tggattgcag taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct
      121 tttaaaattg acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta
      181 actgtcgaac caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac
      241 tatgataatc acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac
      301 aattccacta cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat
      361 tgagcgaata ctaatagggc ttcgaattga tctgttcaat ctttaagcaa tatccctcaa
      421 atgagtttgg aaagtttagt gattcatcac aaatacttaa atacatgtag ttatatatag
      481 attgaggttc ttgtgagcgt atgctcgtgt gggttcaaat cccatcgtct gcatccatct
      541 ttccaaaaca agcggctagg acatttttgt ctcagccgct tgttttctag tttattcttt
      601 catcttgtcg gacctttttt cttgggtata gacttttctc atattctacc acaattttgg
      661 gtttccctaa cacatgatag gatctcacaa caaattgctt tctattatta taataacctg
      721 taacgactaa cttcatccct tcgtctactt cagctaggaa gttcaaacta tgggaagcaa
      781 tcaaacaatt gaccccattt aaactgaaac ggatcaacgg tctggcacta acttgcaaag
      841 tacggatctt atctactgtt ccatattttt tctccataac cttcacctca ataccattat
      901 acgaacatac attccctttg taaagaagaa actctgtttt atctttttga ctttttgtct
      961 attacctttc ttaaaatcat attggcactt cctgcgaaga tgaaggacat gtactaaaaa
     1021 aaagaggcag cagtatagat ttaagtagtt cagttttgga gtacaaaaag tgaaaatgta
     1081 aacctaaatg agtatcaaat tgcaactgaa tttgatactc gttttttatt gttaaatcaa
     1141 cgtttgttgt taatttacac cacaaaatgt aagttaaaat gtatataaaa atgcgaacgt
     1201 gaaataatta ttgtaggaat gcaaattggt gtgttaaaat ggttacaatt tactaagcaa
     1261 aaaggggtgc attttcctat gcaatttctt gagccaaaaa ataagaacgc taagtctgtg
     1321 gattgggaaa tatcagagca agtaagggtg atagttaagc aatacgctga atatgcagag
     1381 cgtactgaaa gcgaagcagt ggatgaattc ttattgaaca ttttagacga taagaagttt
     1441 attgaatgga ttgccaacaa gcgaagtaat aagcggattg ttgaaaaaat gggtatcaaa
     1501 gatagagtgg gttgatagac aatgaccaag cttaaaagat tagccacgaa ggaagatgaa
     1561 attgtagatg ttaaaattcc gatttcaaac gaagaattaa aagatagggc taaacaatat
     1621 gatttattaa ctccaaaacg atttgcaaca aggtataaca aaatgttgtt cttgccaacg
     1681 tcttttaaat ggaacggttc tgaatatccc attcaatata attactgcat caatccgttt
     1741 tgttgtaatt ttgggaaaga acagcacaaa ttcaaagatg taaaagggaa accaagtcga
     1801 tataaaatga ctggtagttc caaagataaa ggtcataaag gtatgtactg caatgacaat
     1861 cctattggca gaggtgtatc gcaaaactgt acagtaaccc ctttatctaa ttggtctgtt
     1921 gttgaagaaa ttaaacgcct tatagaaatc aatagcatcc aagatgttga gccagattat
     1981 caatttcata aagaaggctg ctcggaagaa gaatcaaccc cattcaatga gccgaagcaa
     2041 ttttacaaaa gggggaaaag taggggcaaa tctcaacgct atcaatgtaa ggcgtgtaag
     2101 aagttcacta atgttctacc aaaaagagaa gaaaccacta cttatcacca acaaaagaac
     2161 accattcttc caatgttcgc taagatggtt gttggaaggg tttcagtcag tcgtacttgt
     2221 gatattttag gaattggagt tggaacctac tatcataagc tggagtggct gtataggcgt
     2281 tgtttggagt ttttagaacg atatgagaca caaccccttc aaacaaagaa atttaacgag
     2341 atgtggctta atacggacaa gatgcactac tacctaaata acgtccgtaa gaaaggacaa
     2401 ggttcaaaga aatatacggg gtttgaagac cttaatatgc aaacgtatat agttgtatct
     2461 gctgaggtct tatcaagata tgtgttccgt tctgatgttg cctatgattg gaatatttca
     2521 atggatgaat taaatgaaga tacaagaaaa ttcaaagaag accatctcaa tacttttagt
     2581 cgtaaaaatg ataggttaga ttggtcttat tatccacaag aaccatccgc taacgatagc
     2641 gaaaatagga atgcttatct tcacgaatta gggaaaatta ctaataggag tagatttgtc
     2701 gatggtttaa atgttgatgc tccttatact acaactgctc actattggtt aattaaacaa
     2761 atggttaatg ccgacgaatg gcgaatgatt agtgatgatg acttctctat tagaaacgcc
     2821 ttttatagag tattcacgaa agaattaagg ttgagcgatg cacatcattt tatttgccaa
     2881 gttaacaaga cgaaaagcag aaaacaatgc ttaaaagaat tcggacaagc aaaagcagaa
     2941 ctgctcgatt ggggagatat cagaggattc aaaacaaagt ttctacggac tttggcatct
     3001 cattatctaa ctgaattact aacaagtcat caattccacg aagaggctat tagtaaagat
     3061 ggagaaagat atcgcaaata tgccgataac cctatcaaac atccattagc cactaaggat
     3121 aaaggatttt attcagttga ttgtagaacg gatttatctg ctctggaacc aaacgaaatc
     3181 gcaaagatgc tattaaatgt taatgaccat tcgaccaata gctttattca acagataaga
     3241 cgttatatat catctcttga aagaccatta acaacagctc gtggtgataa gaagagctat
     3301 atttacgcta acttcaatcc gaagtatgct caatttgcga ttacaattct aagaacttac
     3361 tacaactttt gtcgcccttt taaatcagcg gataaaaagg ttttgacacc tgctcaaaga
     3421 ttagggatta cagataaaca atttgattgg aaagatataa tttatttcaa atgacaaaaa
     3481 gagcttggag taatcccaag ctcttgtgct ttatctttac tgttcttctt caattaacgc
     3541 acccgttaac tcaataagtt ttttctttca tatacatgaa aatagtagtt atacggattt
     3601 ttatcattct ttatcccttt ttgggcaaat acctcattcc attcctcata attcacttct
     3661 ggaaaaaaag tatctccttc gaattcatga tgtatttttg tgatgtacat tttctcaaca
     3721 taagggaaaa acaaattata aatctgttct cctccgaaaa taaaaatttc ttcttcgttt
     3781 ttacataact caaaaacatc ttctattgaa tgaacaattt cacaaccatt aaaggtaaac
     3841 cccttatctc tcgtcagaat aatatttctt ctgtcaggta aggctcttcc gattgattca
     3901 aggttcttcc tacctaatat tatcggatgt ccctttgtag tatttttaac atattcccag
     3961 tccttgggaa tcctccaagg aatgtcattc tctttgccaa tcactctatt cttatccatc
     4021 gcagcaatca aagaaacttt catttataac acagcctttt taactaattt accatctaat
     4081 ttcttatttc tatcaaaaaa ccttttctca ttatcctgcc cgttagttga ataagcaatt
     4141 ttttctataa gttatattat cataatacca caaaatcaca aattttcatt caattatcat
     4201 tttcaattgc attttataaa tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa
     4261 aagtagtcca ttttgaagta cattttgtca ttttgtactc caaaactgaa ctacttagta
     4321 tagattactg ttgcttcttt actaatttag gtttacttgt taagaaagat tatttacgca
     4381 aatgttcttc aacaatttgc tgagatttat cagctgtatc ttccacttca tcttttgctt
     4441 tttctaactt ttgaatatat tgtttcaagc ttgagttttc agcagttgct tcgtctaatt
     4501 tcttttgtgt atctgttaac tgagtttcta catttgtttt ttcttgtgtc agtgttgcga
     4561 tttgttgatt caaaccattt acagtatttt gtaattctga aagttttgct tgtgcttctt
     4621 gtagtcttgc tgtccaatct gcattcagat gattgatttc atcatttttt tggttgatct
     4681 catctagttt gacattgatt tcattttgtt tgctttgtag ttgtgcatcc ttgctgacta
     4741 attgactttc taagttttgt ttttctgctg ccgattgatt tttttggttt tctaattcat
     4801 ttacttttgt ttggtattct tggacatctg cctttgaatc ttgcaatgtc tgttcttttt
     4861 gtgctagtcg agcggtaaga tctgtaatcg cttgctcttt atctgtcaat gtatgatcaa
     4921 gctgtgtcag attctcattg attgtatcaa tatctgtctc tccaccccac aaaataattt
     4981 catttgcgta ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg acaatcccac
     5041 cagtaataaa tttctttttc atttgcatag ctccttaatc caatactttt acatgctaaa
     5101 agtaagtgat ctcaaacaca atgtcaagcg tttttaaatt acatatgtaa ttttaatata
     5161 a
//
LOCUS       DACCXW010000154         5150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCXW010000154 DACCXW010000000
VERSION     DACCXW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.160"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(1237..1902)
                     /locus_tag="IVY71_002598"
     CDS             complement(1237..1902)
                     /locus_tag="IVY71_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986617.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2028..4064
                     /locus_tag="IVY71_002599"
     CDS             2028..4064
                     /locus_tag="IVY71_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7986618.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4068..4706
                     /locus_tag="IVY71_002600"
     CDS             4068..4706
                     /locus_tag="IVY71_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986619.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4734..>5150
                     /locus_tag="IVY71_002601"
     CDS             4734..>5150
                     /locus_tag="IVY71_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986620.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPT"
BASE COUNT         1690 a          791 c          942 g         1727 t
ORIGIN      
        1 actacctgta ttagtcctct tttttgacca gattttgacc agtctatttt acattctaat
       61 atattcatat gagctaattt tatgattaaa tggtaagtgt ttccttgttt gttttagttt
      121 aaactagtgt gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa
      181 ggctaggaac ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat
      241 aatttctttt aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca
      301 gagccgaaac agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga
      361 gttggcaaaa gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta
      421 actgtaacaa gctaatttta agagattctt attagaagac gtttaggaat ataactaaga
      481 aaatgattat acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt
      541 taagaaaaag gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat
      601 ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat
      661 aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga
      721 aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt
      781 ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata
      841 aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg
      901 agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact
      961 acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa
     1021 aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt
     1081 ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga
     1141 actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt
     1201 gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg
     1261 gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat
     1321 ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc
     1381 atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa
     1441 gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt
     1501 atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg
     1561 ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt
     1621 tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg
     1681 caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc
     1741 taaagtatga tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca
     1801 actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt
     1861 acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt
     1921 ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg
     1981 aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa
     2041 aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg
     2101 ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt
     2161 atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca
     2221 tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg
     2281 aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat
     2341 tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct
     2401 ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct
     2461 tgatgacgag agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca
     2521 atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa aatagctatt
     2581 cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta actattggat
     2641 tgattctttt attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt
     2701 tattgttgaa taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga
     2761 aaatcattat tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata
     2821 aggagcaatt tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta
     2881 tctctttgat ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact
     2941 atatccaata cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca
     3001 atgtcgaatt agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac
     3061 caaaagaaat cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc
     3121 acgtggattt tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa
     3181 ctttgaataa aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg
     3241 aacagtttgg cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg
     3301 atgtctctgg aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc
     3361 ctgaacgata tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc
     3421 aagaaatcag tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata
     3481 catttattcc ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga
     3541 aaaacagtat gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga
     3601 tcaagatagg ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac
     3661 gtgataactt gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt
     3721 taagcagatc atttcattac ttgatacaga tatttttact cactttcttt tctttcttct
     3781 tgttgagcta tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct
     3841 ttcgttcaag tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca
     3901 tcaaatgggg aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc
     3961 tttttaatat ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt
     4021 ctacagaaaa gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga
     4081 tagtgataaa aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt
     4141 tcactgcgga tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta
     4201 cgttattgaa tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg
     4261 atcaagagac gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat
     4321 cttttctttt tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata
     4381 ttggattgaa gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat
     4441 tgaaaaaagt aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg
     4501 agaagcaaag aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg
     4561 atgaaccgac tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc
     4621 aacaagcttc ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa
     4681 taggaaacaa gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa
     4741 aagcagttat tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg
     4801 aagtggaagc aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga
     4861 ttcattttga tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt
     4921 ctagaacagc aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta
     4981 cgagaaaaac aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag
     5041 cgaaagttgg aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa
     5101 gtgcgacagg aaatagaact gacacattct catgttggta caatccaacc
//
LOCUS       DACCXW010000155         5116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCXW010000155 DACCXW010000000
VERSION     DACCXW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.161"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(406..780)
                     /locus_tag="IVY71_002602"
     CDS             complement(406..780)
                     /locus_tag="IVY71_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7986621.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="IVY71_002603"
     CDS             complement(803..2203)
                     /locus_tag="IVY71_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP7986622.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="IVY71_002604"
     CDS             complement(2207..2617)
                     /locus_tag="IVY71_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7986623.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="IVY71_002605"
     CDS             complement(2635..3126)
                     /locus_tag="IVY71_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7986624.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="IVY71_002606"
     CDS             complement(3136..3927)
                     /locus_tag="IVY71_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7986625.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="IVY71_002607"
     CDS             complement(3920..4693)
                     /locus_tag="IVY71_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7986626.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5116)
                     /locus_tag="IVY71_002608"
     CDS             complement(4849..>5116)
                     /locus_tag="IVY71_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7986627.1"
                     /translation="LYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKE
                     GAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1634 a         1034 c          673 g         1775 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagt
//
LOCUS       DACCXW010000156         5095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCXW010000156 DACCXW010000000
VERSION     DACCXW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.162"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..153)
                     /locus_tag="IVY71_002609"
     CDS             complement(<1..153)
                     /locus_tag="IVY71_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7986628.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFR"
     gene            311..574
                     /locus_tag="IVY71_002610"
     CDS             311..574
                     /locus_tag="IVY71_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986629.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            598..786
                     /locus_tag="IVY71_002611"
     CDS             598..786
                     /locus_tag="IVY71_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986630.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(869..1054)
                     /locus_tag="IVY71_002612"
     CDS             complement(869..1054)
                     /locus_tag="IVY71_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986631.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1269..1640
                     /locus_tag="IVY71_002613"
     CDS             1269..1640
                     /locus_tag="IVY71_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7986632.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1790..1862)
                     /locus_tag="IVY71_002614"
     tRNA            complement(1790..1862)
                     /locus_tag="IVY71_002614"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1827..1829),aa:Gln,seq:ctg)
     gene            complement(1908..2336)
                     /locus_tag="IVY71_002615"
     CDS             complement(1908..2336)
                     /locus_tag="IVY71_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986633.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2396..3154)
                     /locus_tag="IVY71_002616"
     CDS             complement(2396..3154)
                     /locus_tag="IVY71_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP7986634.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3449..3646
                     /locus_tag="IVY71_002617"
     CDS             3449..3646
                     /locus_tag="IVY71_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986635.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3752..4234
                     /locus_tag="IVY71_002618"
     CDS             3752..4234
                     /locus_tag="IVY71_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986636.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4292..4888)
                     /locus_tag="IVY71_002619"
     CDS             complement(4292..4888)
                     /locus_tag="IVY71_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7986637.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         1652 a          790 c          979 g         1674 t
ORIGIN      
        1 cctaaaagaa aaaatttgat aagtaaaata tacagcaggt aaagaggcca gtaaaactga
       61 aaacttcttt tttgtccatt ttatgtaata gaaaagaatg cttatcataa taaaaaaagg
      121 aatataaaaa acaaattgac cgatcgcgat catactattg cctccgataa ataaagtcca
      181 tgatacaaaa taacactttg tgcgaaatgg cgcaagcagt tttgtggatt tatccggtaa
      241 aatctgtatc gattttgaaa ttagaggaga aacttgctat aatcaaagtg aaagaaggag
      301 tgggattttc atggatcatg tagtgttaga aatcgatttg catttgatca ataatattcg
      361 ggtgatttac tatgttgtaa gtaacagtgt cgaacaacgt gtactgacga ataaaataaa
      421 tggtatttta gcaaagaaag acgttcaccg attcaataat ggtgaaggaa gttattatag
      481 tatcccagta gaaaaaatta tttatacgac agtcaaagtc agagaagatc tagataccaa
      541 aaaagcatat gaacctattt ttacaactta ttgattgaca gcagatggag gaaaaaaatg
      601 aatccatatg aacaacttat tcaagatttg atcgatggaa aaatcgaaaa gattgatgta
      661 aaaagagaag aattaatggc ttttcgagaa gcgtggctga agttggagaa tcgtaaatat
      721 gtggtgggaa aagcctcact gaacggcaat gtgacttatc attacgatcc gacacgttta
      781 ttttagtatg aagtattcag aaaagaacca aaggaggacg atgcagcagc attgtcctcc
      841 tttgatttta caaaagtgaa agttttactt atttgaatag atttttaaat ttgtttttga
      901 cactgtcgat atttacatct tttaaaagat tttgggcttt ctctttataa ggtcctaatt
      961 gttctttatg ttcttcgata aatcgttttg cgccatcgaa attgccgttc ttaatcattt
     1021 ccttgacttt ttgaaataat tcttcttgat tcattttgaa cacatccttt gctatcaaca
     1081 tgatttttta catggacaaa acgagaaaaa ctgatttatc cacacgtcaa taatatcaaa
     1141 taacgatcat ttttactaaa aaaacactcg attctcgctt ctaaagtagg agaaaaatct
     1201 ccaacttggg acggatggaa tttctttaac tgacccatat actgaatata taacaaggag
     1261 gtagaaagat gaaagtgatt aaatatttga ttggactggc ggcgattagt ggtattttgc
     1321 tatttggact gaaatattat actgaaggaa gttatggaga aatccctggt atacttgatc
     1381 agctaaatcc attagttgaa aaaggagagg tctatgtaaa gacacaaaaa ccagaggaag
     1441 taaatgaata tggtacagca aggtatgtgc agaaagctgc tgatgccaat gggaaagaac
     1501 gaacggttga atttaatggt ctttctgtgt taaaagaaaa tcattatttg aaaattagca
     1561 ataaaggagc acacatagaa acgtatgagg aagttcctag agaaaaagtt ccggagaaag
     1621 cattagcaat tattgggtaa atcagttttt gtattaagaa tacgcccgat tttattattc
     1681 ataatttaat tatgtaaact aaatacaagt agtaaaaata atacaataat ccccctgcaa
     1741 aaagattgtt cttttacagg gggttcattt tttcagcaag cacatcaaat tgggatggca
     1801 gggttcgaac ctacaattgc ttgattcaga gtcaagtgcc ttaccagttt ggctacatcc
     1861 catttattca acaaaataat catagcactt tcctaaaaaa acgcaagtta tttttcttga
     1921 tcggatagtt gttgctttac tcgtaaaagg cggcgtacta gtttcttttc taatccagca
     1981 ggaagggcaa tcagttctgc tactgacaag acggtgtcca ataactgata ttctttgaca
     2041 ggttcattca taatatgata gatgtttgct aaacgttcaa tatattcaaa gcgagtatac
     2101 tctgttcgag caattagaaa tttcaataaa tcggtggctt tctcgtactc ttgatctgcg
     2161 taataaacaa gagattgacg atacaatttg atttccagtt cattgagggt agcaggtctg
     2221 cgacgctcgt tccactcatt aatagagact ttaccttgaa catttccctt gcagtcacgg
     2281 acaaaaataa tcggaatttc atttggatcc atttttttat gtgtgaattt ttccattatc
     2341 tacgctccct tcaaaggttt ggtatcttga gtataaggga tttttgtttg aattactagc
     2401 ttttctccaa agttagcatc tgtccaccca ttgcagtgaa aatcgccgaa aaatcgttga
     2461 tcggtactgc tcgatttttt acaccatagg gatcgttgac ataaatattt tctttgtcat
     2521 atccagtgac tacacaagca tgaacaagag gagtgactct tacttctcca ttatttgtgg
     2581 tccaagtcat aaaatcagtt tcatctggga cttcaaaatt gatagtcaca gaagtccaga
     2641 caggatttcc tttagatagt gcttttttga tatcatcgag ttgagcatcg gtttttggaa
     2701 aagtgtgata atccgtttgg acgatttgct gagcaaggga ggcaactggg ggaacataga
     2761 cgaccatagc ccagtctccg ccactgatat caccaacaaa tccttcatta ggatcgccat
     2821 gagtcccatc tgcattgaaa acagggacgt aatccaattg ctcagctaac tggtttttag
     2881 ttgtttcata accataaaaa gataaaagca tagaaagcga agtgacttca cagccatttt
     2941 ctaaagaagg agaatcaaac tggctctcta atggaacatc tagtaaaact tctgctttct
     3001 cttttgtctt tttcgttttt tgtgttgaac ttttttgtgc agtgttcatt gtatgttctg
     3061 gagaatggat atgtaggtaa taacttgtac cgcttaataa aagagcagct gtgcccccta
     3121 gaaacaatag ttttttgaac gtttttttct tcatttgaaa tctccctttt cagctgttta
     3181 tctttcctat tataaatgta tttttattgg aaatggaagt attatatttt aatatgtagg
     3241 aaataattag tttttaagga atatgtttta attttttgaa aaaaattctt ctaaatacaa
     3301 cagctgtttt aattgtgaaa acgaaaacag tacgctataa tgcctgtaag gttgattttt
     3361 aactgtaaat aaaaatcaat gagttaaatc tccaaagaaa tagcagtggt aatgtggaga
     3421 aaagttccag cagtttagga ggattttgat gacggaagaa atgattaatt taggagaaca
     3481 atatgcatgc aaaccaattg gttttacaaa aacggtcatt ggtgaagttg tatcaaaaat
     3541 gacaaactgc gccgtggtca aagttgccca atgtgctgca gaggaccaag aattattaga
     3601 tgaaaaagca agcatggttg ttgcaaaata tgatacattt gaataaagaa aacaattgga
     3661 cgtgcggcgt gctgcaagtc caatttcttt gagaaggtaa atcagtattc ttttcctatt
     3721 ttctggagaa gaaaaagcga ggcgagttgt catggtaaca ttctttacgg ttctattgtt
     3781 tttattcgtt gttgtgatgt ttttatttct ttggctggtt cgaaaagaaa ttatttatcg
     3841 gactgtgcga aatcgatggg tctacttggt gatacccttc ttagtgtcac tagtcatttg
     3901 gtatacactc atcagtcatc ctacagcaga tgaattggca aaagggatct tgtcagcaat
     3961 gatttttatt agttttttgt tggacagtag aggaatcaca gaagaaggac ttgttttgaa
     4021 tagctttgac aaaaaaggtg ttcctttttc tgagatcaat aaaattgtat tatatcagcc
     4081 aaaaggttct aaaatcgtaa aaatgaattt tttccgaaac ggatggcgag gaccgatgct
     4141 gaaattttct gcatcattag aagaattagt tccatttttg tcccaacgct taaacgatga
     4201 ggcagagatt gatattatga ttgatccaga ataaaaacag aggttgcgac aagatgtttg
     4261 tcacaacctc tgttttttat ttcaaataaa attatccaaa tacgcggaag cgatcggcat
     4321 cgttcatgta ttctttttcc tcagccggtg tttctggtga accgaatact aattgcgaac
     4381 gtagtttcca gtttgaaggg atattccatt cttttgcaac tgcttcgtca ataactgggt
     4441 tgtaatgttg caagtttgcg cccaatcctt tatcaactaa tgctacccaa gtattagctg
     4501 ttgcgattcc gtttgattgt tctgaccaat ctgggaagtt gtctgcatat aattcaaatt
     4561 gttcttgtag accttttacc acgtcagtat ctttgaagaa taatactgta ccataaccat
     4621 ttttgaaacc agctaatttg ttttgtgtat ttgggaaagc ttctgctgga gtcaatgggc
     4681 gaagtgcttc ttcagtgatt tcccaaagtt tttcatgtgc atctccgaaa aggataactg
     4741 cgcgtgttga ttgagcattg aaagcagttg ggctttcttt aattgcttct ttgatcaaag
     4801 ttgttaattc ttcattagaa agtgaaacat tacgtcctag atgatagatt gaacggcggt
     4861 ttttcaaagt gtcagtaaaa gttgtcataa taaaatttct cctttgattt gcgttttgca
     4921 tttttttaat tgattacgaa atgaagtata gcaccttaca aaaagtaagt aaacagatat
     4981 gctcacaaaa aataagcgaa gttcaaaaaa caattttaat aagaaaaatc gttatgtaag
     5041 gattttctta gttatcgctt tcattgttgt gttttaggaa tttttgctta tttga
//
LOCUS       DACCXW010000157         5011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCXW010000157 DACCXW010000000
VERSION     DACCXW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.163"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..62)
                     /locus_tag="IVY71_002620"
     CDS             complement(<1..62)
                     /locus_tag="IVY71_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7986638.1"
                     /translation="MKTLDAIALTLLIVGGLNWL"
     gene            286..1086
                     /locus_tag="IVY71_002621"
     CDS             286..1086
                     /locus_tag="IVY71_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7986639.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1105..2973
                     /locus_tag="IVY71_002622"
     CDS             1105..2973
                     /locus_tag="IVY71_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7986640.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            2966..3457
                     /locus_tag="IVY71_002623"
     CDS             2966..3457
                     /locus_tag="IVY71_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7986641.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3445..3999
                     /gene="srlA"
                     /locus_tag="IVY71_002624"
     CDS             3445..3999
                     /gene="srlA"
                     /locus_tag="IVY71_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7986642.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4017..>5011
                     /locus_tag="IVY71_002625"
     CDS             4017..>5011
                     /locus_tag="IVY71_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7986643.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1751 a          789 c         1041 g         1430 t
ORIGIN      
        1 aacagccagt tcaaccctcc cacaatcaat aaagttaatg caatagcatc taaagttttc
       61 attagaattc tcctttctta aatgataaaa caattatata tcaaaaagag aaacattgct
      121 tatataacgc tcaggaattt tagcaatcta tgcaaaaaga tgaaaatttt ttttgctctt
      181 ccattagtac acatttttta gctgaagctg taaatttcat gtcttaattt tttttagtga
      241 gcttttatac tggatttgta agaaaaaaga aggagaggat cacatatgtc ggattggttg
      301 gatattgcag gaaaagtagt aattgtaact ggaggttcct caggaattgg aagaagtatc
      361 gtcgaaaatt tgttgaagca gaatgcacaa gtagcgaatt ttgatgtaac tgaatgccgt
      421 atacagcatg aaaacctact atctttaaaa gtcgatgtgt cttctaaaac ggacatagaa
      481 gaagggatat ataaagtaat gaaacatttc aagacgatcg atggtctagt caataatgca
      541 ggaataaata ttccttctct tctaatagat agaaatcatc caaaaagcaa gtatgaatta
      601 tcagaacaag tttttgataa gatgatcgct gtcaatcaaa aaagtgtata tttgatgagc
      661 caagcagttg gaagaatctt ggtgcaaaag ggatcaggag tgatcgtgaa tctttcttct
      721 gaatcaggat tggaaggatc agaaggacag agctgttatg ctgctacaaa agcagcgatg
      781 aacagtttta ctcgttcttg ggcaaaagag ttaggaaaga ggaatgttcg tgtggtaggt
      841 gttgcaccag ggatactgga agaaaccggt ttgagaacac aagaatatga agaagctctt
      901 tcttatacga gaggtataag cgtcgaacag ctgagaaacg gttacagtcg aacaagtacg
      961 atccctctag gaaggagtgg taagctgcaa gaagtggcgg atctagtctg ctattatctt
     1021 tcagaaagat caagttatat cactggagtc acgactaata tttcaggagg taaaacaaga
     1081 ggataaagga taggggggac gcacgtgacg ctagtaaaca gatggtacca attattgaaa
     1141 ttactagtca ctcacaagaa agtttcaatc caggaagtgc aaaaagaaat caaaaccagt
     1201 aatcaaacca taaaaaaaga tattggggag ttgaatgaag aaattgccgg tattgcaaaa
     1261 atcatccaaa aaaacaaaca ttttgaactt gaaatttatg attttgattt ttttgatgag
     1321 atcatgcaag gaaaattgaa aacagccagt gatttcaatt cggcatccaa aagaatcgct
     1381 tatttactaa ataggctgat cgaaacagag gatttcttac gtatggatga tttgtctgaa
     1441 gaagtcggtg tcagcagagg aacagtggtc aaggatctaa agacattaaa agagatgatc
     1501 aatgattttg atgtaaagat tacaggcaca ccaaataaag gactaagaat aataggagat
     1561 gagctagaat tacgactact tttattttat tttgtttctc cctacttttc agagacgaaa
     1621 acagaacagt ttctagaaga aaaacaagca atcaaacatt ttcagaagaa gacgaatgct
     1681 agtaaacaag aaatcaccct tttaacgaaa gttatcggta tttctttgaa tagaatctct
     1741 agtaactatc tgttgaagaa gcccatcaat cgttacagtg ggtgttttga atatatccaa
     1801 gaattcaatg aactgatcaa tcagttggaa gaaatctatg aactaacttt gagccaatat
     1861 gaaaaagatt ttatcagttt tcctttgaac atcatcaaca caagtataac gatgataagg
     1921 aaagctaact ctgatttgcg cttatatttc gaccccatga tgatgcgtat tcgacgatta
     1981 gatctcattg atctggatga gtccttctta tatcaagaga tgaaacagca cctctcttta
     2041 ttgataaatc gggtaatttt tcgttatcga ttgactgatt tattttatgg tgagatagaa
     2101 aaaaagtatc ctttttctta ccaaatcgct agcgaatgca tacaagcatt agaagctatc
     2161 ttgcaacata agatctctaa agtagagatc agctacctaa cgttgtattt ggagttagca
     2221 tttcgatatc aaaaagagca tttttcctct aaaaaagtag ccattgttca tacgacagga
     2281 aaaggaacag cattaatgat ccaaagacaa atcaaaagga tattggggag ggatatacaa
     2341 ataacacatg tttcagaaga acaatatgaa aaagtagatc taaacaaata tttcgctgtt
     2401 tttacaacga ttccgttaaa aaatgtaaat gaagagacac cggtgatcca tttgactaat
     2461 ctttttgatg actcttggtt aagggcacaa tggcaaaaag caaaaaaaaa gcgattagcc
     2521 gggtttcatt ctactgtttt tttctatcaa atatggaaag aaaaaaagac gtatcaagaa
     2581 tatttaaaca aattagtcgc tcctttgatc agaggctatt atgtggatga agaatttgaa
     2641 aaaaggatct tgaaaagaga atatcagcaa agcacatttg ttaacaaaga atttgctttt
     2701 cctcacacaa ccaaccagct atctcaacac attgttttat cagtagggct aataactgat
     2761 caaggtttag cagaaacagg aggaaatgga gttatttttt tatttgccat acctgaagaa
     2821 atgacggctg aagttgagaa tgaattattt gagctatacg atatcctatt tgaactcata
     2881 aaaatgggaa tagatgagtt tattgaatta gcaccgacac aagaatcatt tttaaaacta
     2941 atcaaaagta aaggagagag acttcatgaa tgatatgtat ctattcatag ttttcgcagc
     3001 ttcagcttat gtgctgcaag cagttatcag ttggaagcaa ctaaagcatt tcaatcaaac
     3061 atatagcgaa ctgcgaagaa aaggaaaagt agcgattggt aaacgacctg ggaaaatccg
     3121 ttcaggcacg attgttatgc tagctataga cgatcatgat caagttttag aagtcagaaa
     3181 aatgcaaggg gttacatctt tggcaaaatt tcattcatta cctgtctatg cgggtgaaga
     3241 tatccattac atcgatcgtt accatccctc agttcgagcg gaagatcgtt tgacacaagc
     3301 agctttagaa aatgccagag aagtatactt acgaatagag gcaggaaatg aaaaaagtga
     3361 acaaccttca ttgcctttta ttgctatgct caataatttt cggatatgga aaagtacgat
     3421 caaagaaaaa aggagtgaca aacgatggat atcataacaa aatttgctga gggatttatg
     3481 aggctgttcc aaacaggtgc agaaacattc ataagctgga tgacaggaat cgtaccagtt
     3541 gtcttgatgc tgatggttgc aatgaatacg ctgattgctt ttttaggtga agaccgagta
     3601 acgaaagtag ccaaattatc agcgaaaaat ccgcttactc gttaccttgt gttaccgttc
     3661 ttatctgctt ttatgttggg aaatcctatg tcatttacaa tggctcgttt ccttccagaa
     3721 tactacaaac caagctatta tgctgctcaa gctcaatttt gtcatacgag caatggtgta
     3781 ttcccccata ttaatcctgg agaattgttt gtatggatgg ggattgctca agggatcgaa
     3841 accttagggc taaattcgat ggagctggct atccgatact tgcttgtggg actgctgatg
     3901 aactttattg gtggttggat aactgatttt actactggat ttgtatgcag acagcaagga
     3961 atcgttctta gcaaaaaagt agagctttca gttgattaga gaaaggagag ttttaaatgt
     4021 atggtgatat taagatcgta agaggaaacg gcgggtatgg aggtcctttg attattaggc
     4081 cgacaaaaga taaacacaag ttcatctatg tcacaggtgg aggtgagagg ccaatgatcg
     4141 tagataaaat agaagaactg acaggtatgg agtctatcaa tgggttcaaa acatcaatac
     4201 ctgatagtga aatcgcgctt gcaatcgttg actgtggagg gactcttaga tgtggaattt
     4261 atcctaaaaa agggatacca accatcaata tcgtttccac aggaaaaagt ggcccattag
     4321 cacaatatat cactgaagat atttatgttt cagcagtagg gttagaacaa atacagccac
     4381 taatggaaac tgatgataac aaagaaacag taaatactgc atctccagat aaaaagtaca
     4441 aatatgatac ttctaaaaag ataaccgagc aaaaagcaga gcagcaaaac tggatagcaa
     4501 aaattggaat gggcgcggga aaagtagttg ctacattcaa tcaatcagca agagaagcag
     4561 tagatacgat gatccggaca gtgatccctt ttatggcttt cgtttccctt ttgattggtg
     4621 tgatacaagg ttctgggatc ggtaatgtat ttgctaacat tatggcacct ttagcaggaa
     4681 atgcagttgg attaacaatc attggcttta tttgttcact gccattttta tctcctttgc
     4741 ttggtccagg ggcagttatt agccaagtga ttggaacatt gatcggtgta gagattggga
     4801 aaggaaatat tcctccccaa ttagcattac cggcactatt tgctatcaat actcaaaatg
     4861 catgtgattt tattccagtc ggtctaggtt tggaagaagc aaaagctgag acagtagaag
     4921 taggtgtacc atcagttctt tattcaagat ttcttaacgg catcccacga gttatcattg
     4981 cttgggtagc aagttttgga ttatatagct g
//
LOCUS       DACCXW010000158         4995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCXW010000158 DACCXW010000000
VERSION     DACCXW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.164"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            72..494
                     /gene="mgsA"
                     /locus_tag="IVY71_002626"
     CDS             72..494
                     /gene="mgsA"
                     /locus_tag="IVY71_002626"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7986644.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            817..1353
                     /locus_tag="IVY71_002627"
     CDS             817..1353
                     /locus_tag="IVY71_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7986645.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(1393..3156)
                     /locus_tag="IVY71_002628"
     CDS             complement(1393..3156)
                     /locus_tag="IVY71_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986646.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3159..4874)
                     /locus_tag="IVY71_002629"
     CDS             complement(3159..4874)
                     /locus_tag="IVY71_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986647.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         1526 a          973 c         1009 g         1487 t
ORIGIN      
        1 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
       61 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
      121 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
      181 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
      241 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
      301 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
      361 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
      421 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
      481 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
      541 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
      601 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
      661 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
      721 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
      781 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
      841 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
      901 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
      961 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     1021 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     1081 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     1141 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     1201 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     1261 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     1321 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     1381 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
     1441 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
     1501 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
     1561 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
     1621 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
     1681 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
     1741 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
     1801 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
     1861 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
     1921 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
     1981 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
     2041 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
     2101 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
     2161 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
     2221 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
     2281 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
     2341 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
     2401 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
     2461 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
     2521 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
     2581 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
     2641 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
     2701 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
     2761 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
     2821 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
     2881 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
     2941 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
     3001 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
     3061 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
     3121 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
     3181 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
     3241 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
     3301 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
     3361 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
     3421 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
     3481 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
     3541 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
     3601 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
     3661 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
     3721 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
     3781 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
     3841 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
     3901 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
     3961 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
     4021 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
     4081 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
     4141 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
     4201 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
     4261 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
     4321 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
     4381 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
     4441 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
     4501 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
     4561 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
     4621 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
     4681 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
     4741 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
     4801 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
     4861 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
     4921 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
     4981 aagttttatt atgaa
//
LOCUS       DACCXW010000159         4903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCXW010000159 DACCXW010000000
VERSION     DACCXW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.165"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IVY71_002630"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IVY71_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7986648.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="IVY71_002631"
     CDS             complement(2066..3064)
                     /locus_tag="IVY71_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7986649.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="IVY71_002632"
     CDS             complement(3290..3943)
                     /locus_tag="IVY71_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7986650.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IVY71_002633"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IVY71_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7986651.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1450 a         1021 c          753 g         1679 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aac
//
LOCUS       DACCXW010000160         4771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCXW010000160 DACCXW010000000
VERSION     DACCXW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.168"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(207..1169)
                     /locus_tag="IVY71_002634"
     CDS             complement(207..1169)
                     /locus_tag="IVY71_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7986652.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IVY71_002635"
     CDS             complement(1171..2610)
                     /locus_tag="IVY71_002635"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7986653.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IVY71_002636"
     CDS             2795..4741
                     /locus_tag="IVY71_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP7986654.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1516 a          817 c          790 g         1648 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt t
//
LOCUS       DACCXW010000161         4737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCXW010000161 DACCXW010000000
VERSION     DACCXW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.169"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            150..956
                     /locus_tag="IVY71_002637"
     CDS             150..956
                     /locus_tag="IVY71_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP7986655.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            974..1801
                     /gene="vanW"
                     /locus_tag="IVY71_002638"
     CDS             974..1801
                     /gene="vanW"
                     /locus_tag="IVY71_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7986656.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1798..2769
                     /locus_tag="IVY71_002639"
     CDS             1798..2769
                     /locus_tag="IVY71_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7986657.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2762..3790
                     /gene="vanB"
                     /locus_tag="IVY71_002640"
     CDS             2762..3790
                     /gene="vanB"
                     /locus_tag="IVY71_002640"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7986658.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3796..4404
                     /locus_tag="IVY71_002641"
     CDS             3796..4404
                     /locus_tag="IVY71_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7986659.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1331 a         1002 c         1262 g         1142 t
ORIGIN      
        1 ttgaccgaca ggtataaccc tgccggtctt tttttgctgc tacaggaaaa ctacagactg
       61 gctacaggga aagtacagat gcgcttgcca taataacaat cgtaccagcc acaaatcgta
      121 gttttattgc aaaggaggca ttcgatcaaa tggaaaaaag caactatcat tccaatgcgg
      181 atcatcacaa acggcatatg aaacaatccg tggaaaagcg ggcttttcta tgtgcgttca
      241 ttatttcgtt cacagtctgc acgctgtttt tggggtggag actggcttcc gtattggagg
      301 caacacagat accgcccatc cctgcaactc atacaggcag cagcactgac gtagtggaga
      361 atttggagga aaacgctctt gccaccgcca aagaacaggg agatgaacag gaatggagcc
      421 tgattttagt gaacaggcag aaccccatcc ccgcacagta cgatgtggag cttgagcaac
      481 tatcaaatgg tgagcggata gatattcgga tttctcccta tcttcaagat ttgtttgatg
      541 ccgcaagaac tgatggagtt tacccgattg tcgcatccgg ataccgaaca acagaaaaac
      601 agcaagaaat tatggatgaa aaaattgccg aatataaggc gaaaggctac acctctgcac
      661 aggctaaagc ggaagcagaa acttgggtgg ccgtgccggg aacgagcgag catcagcttg
      721 gtcttgctgt ggatatcaat gcggacggaa ttcattcaac aggcaacgag gtttatagat
      781 ggctggatga aaacagctat cgttttggtt ttattcgtcg ctacccacca gacaagacag
      841 agataaccgg tgtgagcaac gagccgtggc attaccgata tgttggcatc gaagctgcca
      901 cagagatgta caaccaaggg gtttgccttg aggaatattt aaaaccagaa aaatgagaaa
      961 aggatgttat gttatggaca gaaaaagatt gacacagcgt ttcccgttcc tgcttccaat
     1021 gagacgggcg cagagaaaaa tgtgctttta tgcgggaatg agatttgacg gctgtcgcta
     1081 tgcgcagacg ataggggaaa aatcgctttc ccatttgctc tttgaaacgg attgcgcatt
     1141 atacaaccac aataccggat ttgacatgat ataccaggaa aacaaggtgt tcaacttaaa
     1201 gctggcagca aagaccttaa acggcttatt gataagaccg ggggaaacct tttctttctg
     1261 gtggctggta cgccatgcgg acaaagatac cccctataaa gacggcctta cggtgaccaa
     1321 tggtaaactc accaccatgt cgggcggcgg tatgtgccag atgagcaatt tactattttg
     1381 gatgttcctg catacgccat tgacaattat ccagcgcaga ggtcacgaag taaaggagtt
     1441 tccagagcca aacagtgacg agattaaagg ggtggatgca accatttcag agggctggat
     1501 tgatttaaaa gtgcgaaacg ataccgactg cacctaccaa atatgggtga ccttagatga
     1561 tgagaaaatc atcggtcagg tgttcgccga caaagagccc caagcattat acaaaattac
     1621 aaatggcagt atccagtatg tccgtgaaag tggcgggatt tatgaatatg cccaggttaa
     1681 acggatgcaa gttgccttag gtaccgggga aataatagat tgcaagctgc tttatacaaa
     1741 caaatgcaaa atctgttatc ccctcccgga aagtgtggat attcaggagg agaaccaatg
     1801 agaaaaagtg tgggcattac tgtttttgga tgcgaacagg atgaggcaaa tgctttccgc
     1861 gctttatcgc cggattttca tattatccct acgctgatta gcgatgcgat atcggcagac
     1921 aacgcaaaat tggccgctgg caatcaatgc gttagcgtaa gccataagtc cgaggtttcc
     1981 gaggcgacaa ttcttgcgct gagaaaggtc ggggtaaaat acatttctac ccgcagcatc
     2041 ggctgcgatc acattgatac gactgccgcc gagagaatgg gaatctcggt tggcacggtt
     2101 gcgtattcgc cggacagcgt tgcggattat gctttgatgc tgatgctgat ggccatacgg
     2161 ggtgcaaaac ccaccatgca cgccgtggcg caacaagatt tcagattgga tcgtatccgg
     2221 gggaaagaac tgggggatat gactgtggga gttattggaa ccggccatat cgggcaagcg
     2281 gtcgtcaaaa ggctgcgggg atttggatgc catgtgctgg cctatgataa cagccgaaaa
     2341 atggatgcag attatgtcca gcttgatgag cttctaaaaa acagcgatat tgttacgctc
     2401 catgtgccgc tttgtgcgga tacccgccat ctgatcggtc agaagcaaat tggagagatg
     2461 aagcaaggcg catttttaat caacaccggg cgcggagcgc ttgtcgatac cggggcgctg
     2521 gtggaggcgt tggaaagcgg aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag
     2581 gatcagtttg tttataccga ttgctcgcag aaagtgcttg accatccctt tttgtcgcag
     2641 cttttaagga tgccgaatgt gatcatcaca ccccatacgg cgtactacac tgagcgtgtg
     2701 ctgcaggata ctacagaaaa aacaatcagg aattgtctca attttgaaag gagtttacag
     2761 catgaataga ataaaagtcg caatcatctt cggcggttgc tcggaggaac atgatgtgtc
     2821 ggtaaaagcc gcaatagaaa ttgctgcgaa cattgatacg gaaaaattcg atccgcacta
     2881 catcggaatt acaaaaaacg gtgtatggaa gctatgcaag aagccatgta cggaatggga
     2941 agccgacagt ctccccgcca tactctcccc ggataggaaa acgcatgggc tgcttgtcat
     3001 gaaagaaagc gaatacgaaa cacggcgtat tgatgtggct ttcccggttt tgcatggcaa
     3061 atgcggggag gatggtgcga tacaggggct gtttgtattg tctggtatcc cctatgtggg
     3121 ctgtgatatt caaagctccg cagcttgcat ggacaaatca ctggcctaca ttcttacaaa
     3181 aaatgcgggc atcgccgttc ccgaatttca aatgattgat aaaggtgaca agccggaggc
     3241 gggtgcgctt acctaccctg tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt
     3301 aaccaaagta aacggtacgg aagaacttaa cgctgcgata gaagcggcag gacaatatga
     3361 tggaaaaatc ttaattgagc aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg
     3421 gaacgaggat gatttgattg tcggcgaagt ggatcaaatc cggctgagcc acggtatctt
     3481 ccgcatccat caggaaaacg agccggaaaa aggctcagaa aatgcgatga ttacagttcc
     3541 cgcagacatt ccggtcgagg aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg
     3601 ggtgcttgga tgcagagggc ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat
     3661 cgttctaaat gaggtcaata ccctgcccgg ttttacatcg tacagccgct acccacgtat
     3721 gatggccgcc gcaggaatca cgcttcctgc actgattgac agcctgatta cattggcgtt
     3781 aaagaggtga taagtatgga aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc
     3841 cgttgggatg ccaagtacgc cacatgggat aacttcacag gaaaaccggt ggatgggtat
     3901 gaggtgaatc gcatcatcgg cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc
     3961 catgcggctg cgcttggcta tggcttgctt ttatgggatg gatatcggcc aagaactgcg
     4021 gtggactgct tcctgcgttg ggcagcgcaa ccggaggaca atctcacaaa agaaaaattt
     4081 taccccaata tagagcgagc cgagttgatt acaaagggtt atgtggcctc acaatccagc
     4141 catagccgtg gaagcgcaat tgatcttacg ctctaccacc tggatacagg ggaacttgtt
     4201 tcaatgggaa gtaacttcga ttttatggac gaacggtcgc atcatacagc aaaagggata
     4261 ggggatgcag aggcacaaaa tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt
     4321 cagtcttatc gctttgaatg gtggcactat aagttgattg atgagccata ccccgatacc
     4381 tattttaatt ttgccgtttc ataatgaaag tattggtttt gtatcagatt ttggatttga
     4441 taaaatctat gctggtcatg gacaatcttt tactggaaag tggttaaagc atcttttgta
     4501 atacattgca aattatagta ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt
     4561 gggcagacct ttcccacgcc cattttgtat tttgctggcg gcggttttga gaaatcaagg
     4621 ccgccgttct tttctggcag caaaaggtgt ggcggcccat aggaggatgc cgctatgctg
     4681 ttttgctatt gccatgctct aatccacttc caccactcaa aaaaagcctt gcccctc
//
LOCUS       DACCXW010000162         4706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCXW010000162 DACCXW010000000
VERSION     DACCXW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.170"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..506
                     /locus_tag="IVY71_002642"
     CDS             <1..506
                     /locus_tag="IVY71_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7986660.1"
                     /translation="FSDKKLINSIRKLNDAQVLELQTLKPNDLVVIRLELNGKKVSLS
                     NASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKKENRQILIVT
                     HNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGGEDAFTNRAKRY
                     GFKQIVE"
     gene            556..945
                     /locus_tag="IVY71_002643"
     CDS             556..945
                     /locus_tag="IVY71_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7986661.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            932..3277
                     /locus_tag="IVY71_002644"
     CDS             932..3277
                     /locus_tag="IVY71_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7986662.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
     gene            3370..4557
                     /locus_tag="IVY71_002645"
     CDS             3370..4557
                     /locus_tag="IVY71_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7986663.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         1511 a          798 c         1013 g         1384 t
ORIGIN      
        1 ttttttcaga taaaaaatta atcaattcta taagaaaatt gaatgatgca caagttcttg
       61 aattacaaac ccttaagcca aatgatttag tagttatccg acttgaacta aatggaaaaa
      121 aagttagttt atcgaatgct agtgctggtc aaaagacatc tgcaattctt tctatgattt
      181 tggcgtatgg aaattcacca ctcatattgg atcagcctga agatgatttg gatagtcaat
      241 taattaataa cttaatcgta agaagtatca ttacaaaaaa ggaaaacaga caaattttaa
      301 ttgtaactca taatgcaaat atcccagtta atggtgattc agaatggttg attagcatgg
      361 gagattcaaa agaaatatcg gctgatcagt gcgggtcggt agatgaaccc aatatcaagc
      421 caagaatatg tactgtgatg gaaggtggcg aggatgcttt tacaaataga gcgaaacgct
      481 atggttttaa acaaattgta gaataacact agagggtgag acatattatt atgtccgccc
      541 tttttaggag gaaaaatgaa aaaaataaaa agctatacca gtatctgggc agttgaaaaa
      601 gtcatttacg caatcaatga ttttcagttg ccttttcctg tgacttttaa ccaaatggct
      661 tggttcgttt tatctttact gtttgtcatc gtatttgccc atgtgcctcc actatctatg
      721 atagaggagg tctttttaaa atacttaggc ataccggtgg ctgtcacttg gtttatgtcg
      781 caaaagacgt ttgatggcaa gaaaccactg ggatttttac gatcctttat cagctatcac
      841 ttgcgattta aggtgacttt tgcaggaaag aaagtcaaag aacagaagaa acgatgggat
      901 gaacccagta ctcttgtaag gagtgtgaac tatgtatccg attaagtata ttgaaaataa
      961 tctcgtcttt aaccaagaag gcgagtgttt tgcctactat gaattagtgc cttacaatta
     1021 ctcctttttg tcacccgaac agaaatttca agtgcatgat aactttcgcc aactaattgc
     1081 gcaaaatcgt gaaggcaaga tccatgcgtt acaaattgcc acagaaagta gtattcgcgc
     1141 cacacaagaa cgatcaaaaa aagaaattac cggtagactc aaagaagtag cgaaacaacg
     1201 aattgatata cagacagatg cattagtatc catgattggc gatagtcaga ttgattatcg
     1261 cttttttatt ggctttaaac taattgcgac agatgaagaa gtcaatctaa agagtctcaa
     1321 gaaatctttc ttttctgggc ttcaagaatt tgtctatgga gtgaatcatc atttgatggg
     1381 cgattttgtt tccttatcca atgaagaaat tcgctgctat tctaagcttg aaaaattaat
     1441 ggaaagcaaa ttggctcgaa gatttaaagt caggcgagtc acaccgagtg acctgacgta
     1501 tttgattgaa catatttatg gcgaaaaagg gataccgttt gaagaatacg aatttcagct
     1561 accaaagaaa aagctaaaat cagaaacact ggtgaaacgt tatgacttat tacgtccaaa
     1621 tcgttgttta attgaagaaa aaccaaggtg cttacggatg gaacatgaga atcatgagtc
     1681 gtacgtagcg tatttaacaa ttaatacaat tgtgggggag atggaatttc cttcctcaga
     1741 acttttctat taccagcaac aacaatttac attcccaatc gacacaagta tgaatgtaga
     1801 gattgtgacg aataaaaagg ctttagctac cgttcgtaat aagaaaaaag aactgaaaga
     1861 tttagacaat catgcttacc aatctgacaa tgaaacgaat tccaatgtct tagatgcttt
     1921 ggattccgtg gatgaattgg aaaccacctt ggatcagtca aaagaatcta tgtacaaact
     1981 gagttatgtg gttcgagtga gtgccgaatc agtggatgaa ctaaagcgac gctgtgatga
     2041 agtgctcgat ttttatgatg ataccaatgt aaaactagtc cggccctttg gcgatatgat
     2101 ggggctacat gaagaattct taccctcaag taaacgatat atgaacgact atattcagta
     2161 tgttacgtct gattttttgg cggggcttgg ttttggggct actcagatgc taggagaatt
     2221 agaagggatt tactttggct acaatgtgga tacaggtcgt aatgtttacc tgaaaccggc
     2281 attggcttca caaggagtca agggttcagt aaccaatgcg ctggccgccg ctttcctggg
     2341 ttctcttggt ggaggtaaat cctttagtaa caatctctta gtctactatg ctgttttgtt
     2401 cggtggtcaa gctgtgattg tcgatccaaa aggggagcga ggagggtgga aagaaacctt
     2461 gccagaaatt gcggatgaaa tcagtatcct caacttgacg agtaagtcag aaaatcaagg
     2521 tctgcttgat ccttatgtga ttatgaagaa aaagaaagac tcggaaagtt tagcaattga
     2581 tattctgact tttttaacag gaatttcaag tcgagacggg gagaagtttc ctgtgttacg
     2641 taaagccatt cgaacggtga ctcaaagtga tcaacggggg ttattacaag ttattaacga
     2701 attgcgaaaa gacgggtcac caatcgcgga aaatatcgcc gatcatatcg agtcctttac
     2761 cgattatgat tttgctcatt tgcttttctc agacggctcg attacccaat cgattagttt
     2821 agataaacag ctaaatatca ttcaagtggc ggatttggtt ttaccggatg cagaaacgac
     2881 ttttgaagaa tataccacca tggaattgct tagtgtttcg atgctaatcg tcatttccac
     2941 ttttgccttg gactttatcc atagtaaccg agaaatcttc aaaattgtcg atttggatga
     3001 ggcttggagt tttcttcaag tggctcaagg aaaaacgtta tcgaataagc ttgttcgtgc
     3061 tggtcgggcc atgaatgctg gggtctattt cgtcactcaa aatagcgacg atttattaga
     3121 tgaacgttta aaaaataata tcgggttgaa gtttgctttt cgcagtaccg atattcatga
     3181 aatcaagaaa accttggaat tctttggttt ggatcaggag gatcaaagca atcaaaaacg
     3241 cctgcgagat ttggaaaacg ggattacgtc catataattg tgtaaaagtg aagcatcaaa
     3301 aaagccattg atctcagtta ggagtacaat gttttttgca gaaaacatac tcgaatagga
     3361 ctgatatcaa tgactcaagt acattttact tttgaaagcg aagaaattca agcaattata
     3421 aacgaaagcg gtgcgaatga taccgcaaaa acacttatga ctatcatgtt taatcagctg
     3481 atggaagaac aacgaaatca atatattcag gcaaatgcct atgaacgttc agaagaacgg
     3541 caatcacaaa gaaatggtta ctacgatcgt tcgtttacaa ctcgaattgg tacgttagaa
     3601 cttcatgttc cacgaaccag agacggtaag tttagtccta ctatctttga aagatatcag
     3661 cgaagcgaaa aagctttgat cgctgccatg attgaaatgg tcatttctgg tgtctccact
     3721 cgcaaagtaa ctaaaacagt tgaactgcta acggatggtg cgactgtttc taaatcattc
     3781 gtttcaaatc tgatgaaaca gttggatcca tttgtttttg agtggagaaa ccggagccta
     3841 gaaggctcgg aatatccatt tttcatgtgt gatgccctgt atatgaaagt aagggaaaat
     3901 catcgtattg tctcaaaagg tgtatatatt ggtatcggca ttgattctga tggacgacgt
     3961 acgattcttg gttttgatgt tcaagacggc gaatcggaag ataattggga taccgttttt
     4021 caatcgttcg ttcaacgagg tttgttcggt gtaaaactgg tcatttctga tgcacacaaa
     4081 gggttagtta aggctgttcg caagaacttt ttaggtgcga gctggcaaag atgccaagcc
     4141 cactttttga gaaatatttt tgataagctg ccaaagaaag tttcttctga tgtaaaagac
     4201 gagttgaaga gtatctttaa agcctctgaa cttgaattga cacgtgaaag aaaagagcac
     4261 ttcttggaaa aatatggttg tgattcaaaa ttaagtgctg cttgcgacat tttagaaaac
     4321 ggctttgaag atgctattca aattttgtct tttcctgaaa atatccgccg aaggattcgt
     4381 acaaccaatg tgttggaacg gttgaacgaa gagattcgcc ggagagaacg agtgattcgg
     4441 atttttccaa atatcaattc catcactcgg ataattggaa cacttttgat ggaaaaagac
     4501 actgagtggc tggcttctcc acgaaaatat ttagaattta attcgaataa catttagcac
     4561 catgaacctg tgcgagtctc ttctttctga ggagagacat caagagtcgc tgcgctactg
     4621 ctcctgacct ctctcctcag aaagaaaggc atggcagtta ggttcacaac gcaaaacatt
     4681 gtgcttttga tgaggttgaa ctttta
//
LOCUS       DACCXW010000163         4655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCXW010000163 DACCXW010000000
VERSION     DACCXW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.171"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            83..604
                     /locus_tag="IVY71_002646"
     CDS             83..604
                     /locus_tag="IVY71_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7986664.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(1303..1860)
                     /locus_tag="IVY71_002647"
     CDS             complement(1303..1860)
                     /locus_tag="IVY71_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986665.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(2039..2650)
                     /locus_tag="IVY71_002648"
     CDS             complement(2039..2650)
                     /locus_tag="IVY71_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986666.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2763..3020)
                     /locus_tag="IVY71_002649"
     CDS             complement(2763..3020)
                     /locus_tag="IVY71_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986667.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(3617..3895)
                     /locus_tag="IVY71_002650"
     CDS             complement(3617..3895)
                     /locus_tag="IVY71_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986668.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3929..4180)
                     /locus_tag="IVY71_002651"
     CDS             complement(3929..4180)
                     /locus_tag="IVY71_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986669.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1464 a          934 c          664 g         1593 t
ORIGIN      
        1 tctaattttt agacaaacag tctaaatcgt tgcctttttt tgtcagacat gtataaacta
       61 taagcgaaag aggtgtttta acatggatat atcagtgatt gatgcaacga aaacaaatac
      121 acaaaaagga atcctttatg gttcatcgaa tgctcctaaa aaaatggtcg aattcatcaa
      181 cttagcttgt ccgtattgcc gacaatggtt tgaagaatct tatgagctat tagaagaagc
      241 agtccaaagc gggcaattgc agcgagtgat caaattgttc gataaagaaa aagaaagctt
      301 acagcgaggg aacgtgatgc accggtatct cacaatcagc gatggccaaa aagcgatcaa
      361 agagatcaaa caaatattcg atactcaaga cgagtggaaa catctttctc tccaagaagt
      421 agcggatttt gcagtagata aactaaagct gacagaacaa aaagacgaac agctgagcca
      481 agctgtcatc aacgaagctg agcaagcaca tattcgattt gttccaactg tcatcttggg
      541 taaagaaatc tttgatgaat ctatttcaat agaggaatta aaagaattga tccaaacaaa
      601 ataaaaaaat aaggagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
      661 cgtataaacg gcggaagcag aagcaactcc ttcttatttc ttggagttaa acgcttctgt
      721 cccagcctcc ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca
      781 tttcttatga caagctaagc aaattatctc caccaaataa tttattcata tttttgactt
      841 gcgcatacta actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta
      901 atatttatgt acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc
      961 aattctactt caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg
     1021 ttgagctagg tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac
     1081 caaacgcaag atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat
     1141 aaagctaaca actattaact cctctcagct aatagttgct ctctttttta ttcagattca
     1201 tttttgctga tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat
     1261 tacaacattc actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc
     1321 tattggtgga tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc
     1381 taaaccactt ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt
     1441 tacgccatta aatggatacc aattaggatt aggctctctt tttttctgta catctcccat
     1501 agaatagtta gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa
     1561 taagatattt cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc
     1621 catgctacct gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac
     1681 aatcctttcc ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc
     1741 tggtcttggt ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg
     1801 aaacacagct ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat
     1861 tattcgcaca tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa
     1921 atatctactg gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca
     1981 aatcatcagc ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct
     2041 aatcaccaaa attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt
     2101 attgtgtcaa aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt
     2161 ctcctaatat aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat
     2221 gtggttttaa tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac
     2281 taaaatagat tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac
     2341 ttctccatct tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag
     2401 ctatgacagt atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg
     2461 gaaattctcc ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag
     2521 gaaatattaa gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa
     2581 tccaaaatat aatttgtaca attctcattg atccattgta ttccatttta ttccccctat
     2641 tactattcat atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac
     2701 cttgattttt tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt
     2761 gtctatctaa catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat
     2821 gcatctccct cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca
     2881 taagcagata aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact
     2941 gaatttgctg cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga
     3001 tttacgtgga cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga
     3061 actacgcaac aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg
     3121 aatataaaaa ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca
     3181 ttatactgta tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt
     3241 atcaatctat tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc
     3301 ctatgaatct cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta
     3361 attttaccca ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga
     3421 tgatcatttt aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat
     3481 cgcatctagc ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt
     3541 tcggtctcag aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg
     3601 ttgccgatga ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt
     3661 gcagatacca atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct
     3721 taccattaga tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta
     3781 ttttatgaat tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc
     3841 tgataaacca ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt
     3901 ccgagaacga aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg
     3961 gacttagtgt gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta
     4021 aggctatact gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta
     4081 ataatatgaa aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat
     4141 ttttatacaa tgaccattta tttataggct tttctttcat tttacttccc cctactgttc
     4201 tgttcacacg gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac
     4261 ttgctaacta tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta
     4321 ttcatctcat tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa
     4381 gaattattta gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct
     4441 atgatgaaaa gccttggaat acagtagctc ttaattggta atactttttc cattataaat
     4501 aacctctttt cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa
     4561 cttatatttc cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag
     4621 cattacgcct gccagaataa accaattatt tgtat
//
LOCUS       DACCXW010000164         4640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCXW010000164 DACCXW010000000
VERSION     DACCXW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.172"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            1..855
                     /locus_tag="IVY71_002652"
     CDS             1..855
                     /locus_tag="IVY71_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7986670.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            952..3201
                     /locus_tag="IVY71_002653"
     CDS             952..3201
                     /locus_tag="IVY71_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7986671.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3204..4379
                     /locus_tag="IVY71_002654"
     CDS             3204..4379
                     /locus_tag="IVY71_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7986672.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4382..>4640)
                     /locus_tag="IVY71_002655"
     CDS             complement(4382..>4640)
                     /locus_tag="IVY71_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7986673.1"
                     /translation="GITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1608 a          672 c          846 g         1514 t
ORIGIN      
        1 atggaaaatt ctatatattt agtaacagaa cctaacactc agctgaaaga ttgttacttt
       61 aatttttgtg ggtattcaaa aacagaacct agacatagtt ttggaccagc tattcgagaa
      121 caatacttaa tacacatagt gctagaaggt gaaggctatt attcaattaa tgatcaaaaa
      181 tattttctga agaaaggcca aggatttgtt atcccaccag gagtatctac tttttatcaa
      241 gcagatgaaa aaaatccgtg gtcatatgta tggttgggtt taagtggcaa tcttgtagaa
      301 tactatctaa aagaaattgg tgttggaaag aatcatctta cctttaaagt cgaaaattta
      361 aattcattta aaggattaat ttttgaaact ttatcgtatg atcaggacaa tctagtcagt
      421 gaattgtcca gacaaaaaca agtttataaa tttttagaac tcctcttaaa ttcagctagt
      481 tatctgaacg aagggataat tactaaaaag atgaatcctt ttgttcgtca aacaattgaa
      541 ataatacgtg agaatgcgta tcaaaaatta tctgttaaag atattgctgt cagtctatcg
      601 atacacgaaa attatttatc acgattgttt aagaatgata tgggaatatc aataaaagag
      661 tatattagtg aaatcaaaat ttctattgca aacgatctgt tatcatcaac tgaatattca
      721 atccagcaaa tcagtgacaa gataggattc tcaacagtac aatctttttc taaagccttt
      781 aagaaagcaa atggtgtttc accgggagta tatcgaaaac gtagattagg tctaggaatc
      841 attaatggtg aataaactaa acatttgatt ttgataaata ttcgttactt taaacgctta
      901 ctatttatgt gagcgttttt tataatgcaa taaagaaacg gaggagagct tatgtataag
      961 gataatgaaa tgaatattat ttatcgatca gaaaaccaac aatttcattt gtttaatcag
     1021 gagataagtt acatttttaa gatatcggaa gatggtaaat tattgcattt atattatggt
     1081 gagaacttac cagataatga ctatagtcat ctaattgaga tgcatcatcg gcctatgaca
     1141 acctatagaa atgaaaacga tttgctatat tcgttagagc acttaaaaca agaatttcct
     1201 gaatatggta caacagattt tagacatcct gctatttcat tactacagaa aaatggctct
     1261 cgcttatcag attttgtcta tcaagggtac gaagtggaag aaggaaaacc taaactagaa
     1321 ggtttacctg ctacctatgt tgataataat gatgaaagca aaacattgaa agtttatatt
     1381 ttagattcag ttacaaatgt tcaagtagaa ttattatata caatttatag agattatcct
     1441 gttattactc gtagcgcgcg agttatgaat aaaggaaaag atattcaaat aatcgagaat
     1501 attagcagtt tatcattgga tttacccgat gcaaattatg aatggcaaca attatctggt
     1561 gcttggggta gagaaagaac tattaagtcg cgtactttac aacaaggaat tcaatcaatc
     1621 gaatctactc gaggtatttc aagccatcag cataacccat ttgttacttt aaaaagaaaa
     1681 aatactgatg aaaatcaagg agaagctatt ggtgcagcac tagtttactc aggaaacttc
     1741 ttaattcaag cagaagtaga tacatgggat gttactagac tacaagttgg catcaatcca
     1801 tttggattta attggaaact caatccagga gaaatattta cagcaccaga agcgattctg
     1861 gtttattcaa gtaagggttt aaatgccatg agtcaaactt ttcatcaact atttagaaaa
     1921 cgcttagctc gtggtaagtg gcgagaaaaa gaccgtcctg tcttaattaa taattgggaa
     1981 gcaacatatt ttgattttga cgaagaaaag ttggtaaata ttgctaaaaa agcatttgat
     2041 gttggtattg aattatttgt attagatgat ggctggtttg gagaaagaga aaacgattgt
     2101 gcaggattag gagattggca tgtaaatcct aaaagactac ctagaggaat cggcagcctt
     2161 tcagaaaaaa ttcgtagttt tggtatgaaa tttggtttat ggtttgaacc tgaaatggtg
     2221 aataaggata gtgagttata tcgcacacat ccgaattgga ttatttctac acctaatcga
     2281 aaacctaatc atggtaggaa ccaatatgta ttaaattttg ggttagatga ggttgttgaa
     2341 aatattttcg atcaaatgtg taaaatcatt gatgaatcaa acttagatta cattaagtgg
     2401 gatatgaatc gtccattgac tgatgtattt gacagtcact tgtcagcaga tcaacaagga
     2461 gaagtattcc acagatatgt actaggcgta tatcgcttat atgaaaaact tataacaaaa
     2521 tatcctaaca tactatttga gtcttgttca tcaggtggtg ggcgctttga tcctggaatg
     2581 cttcactatg cacctcaagc ttgggctagt gatgattcag atgcaattga aaggttaaaa
     2641 attcaatatg gaactagtat gctgtatcct ctttcgtcta ttggtgctca tgtatcaatt
     2701 gttccaaatc atcaaacaaa ccgtataaca cctataaaaa ctagaggaaa tgtagctttt
     2761 tttggtgcat ttggatatga gcttgactta aatgaattaa gtgaaggcga tttgaggatt
     2821 gtaaaagaac aaattgcctt ttataaaaaa tatagaacca taattcataa tggtactttt
     2881 tatagactat tatcaccatt cgatgatgat aatcaaaccg cgtggatggt tgttagtcat
     2941 gataagaaaa cggctattgt tgctcattat aaaacattaa atgaggtaaa tgctccttac
     3001 cgccgactga aattaaaagg gttagataat gacggacttt ataatgtagg acaaaatagt
     3061 ttgcgatctg gaatggaatt gatgagagca ggactagtct gttcagatgc atctagtgga
     3121 caaataaatg atcagaatcg acgcccagaa acatttgatt ttgattccaa gatttgggtt
     3181 ataacacagg aggaaaagta gagatgaata accaaaaaag cttacttcaa aaaggagcaa
     3241 tcttaagtat ttcactaatg ttaacaagct ctcaagcaat taatggtgtt attccacaaa
     3301 tgaaacagtc tttggggata tcacaaagtc aaagtgaact tttaggaact gcaccaagta
     3361 taactgttat tttgtttatc ttattatcta gttactttgc caaaagaata ggaatgaaaa
     3421 agacaataat tttaggttta ttattagctg gtataggagg aattattcca gtatttacag
     3481 ctaccttcca aaccgttctt attagtcgag taatattagg tgccggttta ggagtatata
     3541 attcattggc tgtgacatat atcagctctt tatactctgg caatactaga gcaacgctat
     3601 taggtattag aaattcaatg gaagctttag gtcagacaat ccttattttt cttgctggag
     3661 ttttagtgaa cattaattgg acggcttcgt ttttagtcta tgcaattgcg tttcctgtag
     3721 ctctcttatt cgcgctaaaa gttcctgaaa ttagagatga aaatagtgac atacctgaaa
     3781 atcatgtaaa agaaaagatg aatccaatgg tttttgccct agttctattt gcaatcttac
     3841 tcgtaatgaa tagtattgct atttctgtac gatttgcatc aattgcaact gaaattaaag
     3901 gggtaaactt taatgctagt aactatttag ctctaatgcc tatcttagga atactagcag
     3961 gatttatgtt tggaccaata aataagtgga taggaaaagg aaccctatac ttgggaatta
     4021 tattctttat aatttcaaat ttgttaattg ctgtgtcaaa cggtaatatg acgttcctgc
     4081 taacgggttt atttttatca agtataacag gatcatggtg cttcccattt atctttagta
     4141 acttagataa agtcactaca aagaatacaa ttaattttgc tacttcattg atttttattg
     4201 gctgtaatat tggtaatttt atagctccta ttgctatgca attagcacaa tttctaacaa
     4261 gaagtacaaa tttgactgct ccattttttg tattcgcagg aatatttatt gtgatcctat
     4321 ttgtaacatt ttttgcgaca aataaaaaaa atactagagt aacccgtggt gctagttaat
     4381 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     4441 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     4501 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtctca actgtttttc
     4561 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     4621 gaacggatat agtaatgccc
//
LOCUS       DACCXW010000165         4607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCXW010000165 DACCXW010000000
VERSION     DACCXW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.173"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            45..467
                     /locus_tag="IVY71_002656"
     CDS             45..467
                     /locus_tag="IVY71_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7986674.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(633..1826)
                     /locus_tag="IVY71_002657"
     CDS             complement(633..1826)
                     /locus_tag="IVY71_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7986675.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2050..2427
                     /locus_tag="IVY71_002658"
     CDS             2050..2427
                     /locus_tag="IVY71_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7986676.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2415..2753
                     /locus_tag="IVY71_002659"
     CDS             2415..2753
                     /locus_tag="IVY71_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7986677.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2743..2856
                     /locus_tag="IVY71_002660"
     CDS             2743..2856
                     /locus_tag="IVY71_002660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7986678.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            2887..3330
                     /locus_tag="IVY71_002661"
     CDS             2887..3330
                     /locus_tag="IVY71_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP7986679.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3592..4407
                     /locus_tag="IVY71_002662"
     CDS             3592..4407
                     /locus_tag="IVY71_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7986680.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4417..>4607
                     /locus_tag="IVY71_002663"
     CDS             4417..>4607
                     /locus_tag="IVY71_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986681.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
BASE COUNT         1573 a          813 c          880 g         1341 t
ORIGIN      
        1 tttcttgtat gtagtatagt aaagtagata caggaggaga aaatatgcag acttttttaa
       61 caaaagtttc tcaaaagaaa attaaaatga ttcatttact gcttgaaaca gaacgttggt
      121 gttcaataga agaactacaa aacgaattga aggtatcaag taagtctatc ttgaattatc
      181 taacagattt agaagaattg ttccaaaaat atcctgataa ggtcattttg aagaatgaga
      241 ataatcggcg cttttctatg gaaaagcagg agaacttccc aatctacatt atttatcttc
      301 atttctacag aaaatcatat aattatcatt tgattgaatt catgtatcaa catcctgaga
      361 aaaatttgga agattatgca gacgctcaat atacgagtgt atccacagtt tttcgttatg
      421 ctaagttatt agtaaagtac tttgaaatga cggacgtcaa agaatagtcg ccgccagata
      481 gaaggaaatt agcagttgaa aaagctgccc tgcatcaagt aatacgacca gccaaacgga
      541 aacagtgtct catgtttttg atttggctgg tcttattacc gcagatgcaa gcttttgcca
      601 cacctctttc ttgtcattta cagactcaac tatcaatgct gcaagctttc ccttaatgct
      661 gtaacaccag taagagttgt caagaccagc agactgacta ggtaaatacc gctcaaaaac
      721 aagattacgg cagttaaact ataatgatcc atcaagaaac cgatgacgat agaagaaaaa
      781 ccaccaacag ctcgtccaag gttcataatg aaattattcg ccgtcgcgcg gatttctgtt
      841 ggatacaaac tactaatcac tgcgccatat ccaccataca tgccattgat aaagtagccg
      901 actatcacag cggctgctac caaactccac tggctatggg ctaacacaag tagatagaca
      961 gataaggatg aacaaattaa aaacaaagaa aaggctagtt taggccccaa tttatccatg
     1021 attatgccaa aagtcatcat ccccaaagac atgcctaaaa tagtactgac catccacaat
     1081 gaagacccag aaaccgaaag ccctaatcga ttttgcataa ttgaaggcag ccaattcatt
     1141 aacccaaaat agccggcaat ttggacagtt accatgatac taaggccaca tgtttgccaa
     1201 gcaagatgtt ttgattcaaa cagtctgcta agtttggttt tcttttcggc agattgtaca
     1261 gatttgaatt cctttggttc agtcaagccg cgacgaatga acaagaccat caacaccggc
     1321 agtacaccta cgacatacaa catcttccaa ccaaacgttg gaatgatcac agcagctaaa
     1381 atcgcagcca aagccgcacc gacctgcgca ccaataccag caaccgaaga agctcgtcca
     1441 atttgttttt ttgaaaaact ttcggaaatc aaggacatac acgccccgta ttctcctccc
     1501 gctcctacac ctgcgataaa gcgaaagaga taaaccaagt agatgtttga agcaaaatac
     1561 atcaataaag aagcaatcga aaacagaagg accgtttgag aaaatacttt cacacgtcca
     1621 tatttatcag ctatgatgcc aaaaaaaata cctccaatga gcatccccaa attagtgatc
     1681 gttgaaatga aacccgcttg cgtaccacta atatgaaaag aagtaatcat tgaagataat
     1741 gaaaaagcta aaaacataat atccatattc tctaaagcaa ttcccgccga tgtcgataat
     1801 aatacctttc gctgatacgt tgtcattttt ataccccttt tcctaaaatt tccctaaatt
     1861 gttaaaagta tatccaaaaa agaataaaaa aacaagactt ttttatttga aataatacac
     1921 aaacaaccga tatagataat ccagcttagt tttaatagaa atgagtattg acttggagta
     1981 cacttaaagt tgtagaataa ataaaataca taagtaaagt ggcaagaaat taagagaaag
     2041 agtgatcaga tgaatatcaa acaagtcagt gaagaaaagg gcatttcggc cgatacattg
     2101 cgctattatg aacgaatcgg gttgattcca ccagttaatc ggacaaaagg cggaatccgt
     2161 gattatacag aagaagacct aaaatgggtc gactttacga tctgtatgcg aaatgccgga
     2221 ttatcgatcg agtctttgat tgaatatatt cgcctatcta gcgaaggaga agcaactgta
     2281 tttgagcggc gtaagctttt gattgaagaa agtgaacttt tgacgaaaga aattgctgaa
     2341 atgcaagagt gtttggaacg tttacaagga aaaatcaaga aatatgatcg tgtgttatct
     2401 agaaataaaa tggaatgtat caagtaaaaa aatcaaggag catcgctatg gagttacgct
     2461 tactaaaata tttttggaca attgcagaag aaggaacaat ctctcaagcc gcagaagttc
     2521 tacatcttac acaaccaacc ttaagccgac aactgaagga gttagaagag gaactaggaa
     2581 cagaactgtt tgtacgcgaa aaccgcaaaa tgatcttgac agaagcaggt tattttctaa
     2641 aaaagccgag cagaagaaat tttagattta acgaatcaaa ccactcaaga attcgagaac
     2701 agaaaaaaac aattattcag cggccgattt acgatcggct gtgtggaagc tgataattcg
     2761 aatacattgg cactattact agaaaagttg atccgtgatt atcccagcgt tcaatttcat
     2821 atccacagcg gaacaagtga tgatatcacg gagtgactag aaaaaagcct gttagattta
     2881 gcgattttgc tagatccagt agatacagag aaatacgaaa cgattttgtt accaagaaag
     2941 gaaaaatggg gattgctcat tttagaaaat tctgttttag caaaaaaaga cgtgataaca
     3001 aaagaagact ttgcgaatgt acctcttttg atctcaaagc gtccagaaat acagcgaatg
     3061 tttgctgact ggttaggcat aaaaaaggag cagttgctga tcacaggaac gtacaatttg
     3121 attttcaatg tatttgcgct aattgagaat caggtaggtg ctgctttagt catagaaggt
     3181 gctgtaagtc atctttcaaa gcgagtgaca ttcgttccgc ttgacccaat gattgaaacg
     3241 aactgcatgc tagtctggaa aaagaatcgg attttatcac ctgtagtcaa tgaattgatt
     3301 caccgtttca aagaagcttt tgaacagtaa taagtatctc taatcgaaga aatttcaata
     3361 caaaagcgag acgtatgaca gtcttgcttt tgtatttttt catttttaaa tcaaaaaaaa
     3421 taaagagatt tacataactt gttaaataag cgcttacaaa cataagaaga acggaaatga
     3481 atcagctata ataaccgttg ttctggattg taatcattac aaagaaatga agattataac
     3541 tttggaggtg ttcgaaatga aagaaaaaaa gcaaaaattc gttcaaaaaa catgcccttt
     3601 attcaatggt tgggtatgat tctgctgatt gtattagctt tattgacgat cattcctttt
     3661 ttatggatgg tttctgcttc attcaaagca aataatgaag tgttcacgat tccgattcaa
     3721 tggatcccta aaacttggca tttagagaat tatctggtta tttggtctcg tattccttta
     3781 gtgacatttt ttaagaatac agctttttta agcgttgtga tcacgttgat tcagttgttt
     3841 acttctagtt ttgctgctta cgggttttct aaactaaatt ttaaagggcg cgatatttta
     3901 tttttagcat atatcggcac gattgctgtc ccttggcagt cttatatgat cccgcagttc
     3961 attatgatga gaaaagtggg tttaacatat acgttgtggt cgttggtgct attacaagcg
     4021 ttcaatgctt ttggtgtgtt cttgttgaag caatactaca gtagtatccc agattcttta
     4081 tgtgagagtg cgcggatcga tggcttatca gagtggggaa tctatcgcaa gatcatttta
     4141 cctcttacaa aaccggcgat tgccagtttg acgatcatca cattcgttaa tacatggaac
     4201 gattatatgg ggccattcat ttatttgtct tctacggaaa ataaaacaat ccagttaggg
     4261 ttgaaaatgt ttattgggct atttgatgca gagtacgcat taatcatggc tgcctcagtt
     4321 gtttctgttc ttcctgttgc ttttatattt ttagcgatgc aaaaatattt tgtagaaggg
     4381 atcgcttcat caggaatgaa ggaataggag gtgattatga aagagcagcg ttttgacaaa
     4441 aatgtttata tggaattctt ttattggttg tatcaatcat tggcaagtag cctttgtttt
     4501 tggtgtgtca atatcttgta tatagcagct gcgttccttt tgtcgataga tggaaggaat
     4561 gtcttttcct ttattttttc tttacttttt attggtccag gttgcat
//
LOCUS       DACCXW010000166         4591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCXW010000166 DACCXW010000000
VERSION     DACCXW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.174"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            190..2859
                     /locus_tag="IVY71_002664"
     CDS             190..2859
                     /locus_tag="IVY71_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7986682.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2950..3570)
                     /locus_tag="IVY71_002665"
     CDS             complement(2950..3570)
                     /locus_tag="IVY71_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7986683.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3684..4223
                     /locus_tag="IVY71_002666"
     CDS             3684..4223
                     /locus_tag="IVY71_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986684.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4220..4558
                     /locus_tag="IVY71_002667"
     CDS             4220..4558
                     /locus_tag="IVY71_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAP7986685.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1423 a          790 c          860 g         1518 t
ORIGIN      
        1 aaaaattttt aagggtggga cttttgattc ctaccctttt tttgcgttta tttttgttat
       61 acgaacaaat tatgaagtta tttattctaa attttatttt tttgtgaaaa ttagactctt
      121 tttaagcaga gaaaaacaag gatatgataa actgaataca acttcataga aatttggtgg
      181 aaaataaata tgaaaaaaaa tttaaagtca ttctttagag aaaacggacg ctacgcagca
      241 gctagtttcc tgattccgtt tttgatttta gcacttgttt atctaagtat cgggatctat
      301 cctggaagca gcagaagtat tttggcaagt gatgcctttt cacagttttc taattttcat
      361 gccagcttcc gaaatgttct tcttggaaag caaagtattt tttatacatg gaatgcttct
      421 ctaggattga attatttatc tttaatttct tactatctag gcgggatctt cacgccactg
      481 gtgttctttt ttccaaacca gatgatgcca gatgcactgt atgttctgac tttgcttaag
      541 gtcggctgtg ccggattggc tttttggttt cttgcacgta cgttcaagat tcctcgctgg
      601 gggcatgttg cacttagcgt cagttacgca ttgatctcat ttatcactgc acattcggag
      661 atcatcatgt ggctggatgc atttgtttat cttccattag tgattttggg gattcatcgt
      721 gttatggatc ttaaacggcc gaaactttta tttatcagtt atttactctt gtttttatcc
      781 agtttttata tgggttttat gattggcgta ttttctttct tatattttat cgcccgtcta
      841 ttgacaaact ggtcagaata taaaaaaacg attgttcctt atggtatcac ttctttactg
      901 gcaggcggcg cttcaatgat cattgtattg ccagcagtgc tggatcttcg gtcaaatggc
      961 gaggcactga gtcagatcac tacgttgaaa acagaagcga cttcttatct ggatctaatc
     1021 atgaaaaata tgattggtgt ttatgacacg acaaaatacg gatcgattcc gttcgtttac
     1081 gcaggtctgc taccattagt cctctgtctg ttttattttg ccagtcggaa agtaccgttg
     1141 aaaaataaat ttttgtacgg cagtcttttc gccatactta tcgcaagttt ctatcttgtt
     1201 ccattgaatt tgttctggca tggtatgcat gcaccgaata tgtttttatt ccgttatgcc
     1261 tatctgttct ctttcttagt gctcctgctt gcaggaaaag gatgggaaaa gatagaacaa
     1321 gacgatcgtg ggctgttgtt aggaatcatt ttggtcgtag ctgctctctt tgcggtggca
     1381 tggggcatca aacctgaagg aagctacacc tatgtgacga tgacttcctt tgtattgaca
     1441 gtggttttct tagggattta tgcttttgct gtgggatttt atcagcataa aaaactttca
     1501 ttcaatcatc tgagtatatt gttattattg atcatgtctg ctgaagcact ggtcaatacg
     1561 aacagtatga tccatggcat tcttgatgat tggaattatg cttcccgcag tttatatact
     1621 gatccttatc ctgacttgaa agctcttgta aatgaaacaa aaaaagattc cgactcgttt
     1681 taccgcttgg aaaatctgga tccagtatct gcaaacgatt cgatcaacta tggttacagt
     1741 ggtatcagtt tgttttcttc tataagaaat cgtaattctt cctcttacct ggataaatta
     1801 ggtttccgtt caagagggac aaatttgaat atccgttata acaataatac tttgctaatg
     1861 gatgggttta caggaatcaa atataacatt tctaaaaacg aaagcacgtt cagtaaatat
     1921 ggcttccgcc aagctggtca aagtggcgac tatacactgt atcagaatgt caatgcattg
     1981 ccactagcat ttactgcacc gttgtccgtt aatgatgttg agcagccagt gacagataat
     2041 ttgaccagtc agacaaactt gatcaataca ttgtctggtt taaatgaaca gtattttaca
     2101 ttctatacac ctactttgaa gaatcagttg aatacgacga tcacaaagac aacgactggc
     2161 gtcacttact cagaaacagc agataaccag ccaaaagagc tcacttggga agtccatgtg
     2221 cctgcaaata cacaagctta cttaagtttg ttcccaacca attttgcaca attagaaagt
     2281 tctagtgcga cgatcactgt caatggtaca tcgcgaaaaa cgcaaatcaa catttctggt
     2341 caatactatg atcttggcta ttatcctcaa gagacaacag tgacatttac tgcaagtttt
     2401 tatggaacga aagaagtaag tttcatggag cctaaagtcg ttggtctaga tgttgtagcc
     2461 tatcaagccg caatgaatca gatcaaagaa aaaggtgtcg aaatggaagc cactggcaga
     2521 aaagcttctg ggactgtaaa taccgctgaa gattccatgt tggtgacaac tattccttat
     2581 gacaaaggat ggaaagctaa aattgatggg aaaccagtca aggtcagcaa tttcaaagac
     2641 gcattcttga tgataaaagt tccagcaggt aaacatactg tcactttcag ttacttgcct
     2701 gaaggattta cgataggtgc aatccttttt gtcctatgta ttgcattgtt tattctttat
     2761 gtctggtacc ttgaccgcaa tcgccccttt tatggcacga ttgatccagc aatagaagaa
     2821 aaaccgcgaa atacacacag aagaagacga agaaaataaa gaaacgatgg aatctgtgtc
     2881 actcttctat ttaaatggca aaaatttcaa tctttaaatg acaaaaacca cctgattttt
     2941 ggtggttttt tatataccta tgactaaact atctcttgct gcttcgacaa cttctacagt
     3001 gatcttatcc atttgattga ttcttaaaat ccgatcaatc tgcgcaaata aacgatgaat
     3061 aagcctaaaa tttccttttg tgatcttaat gatagttgtg atagcctcat aatcagaaaa
     3121 atcttctagc ttgatatcaa ttcctaaatc tgcccatttg tattccaaaa tatgatgtgt
     3181 ttcgtcctta ctaaggttat caaattcatg tgcaaatcct atgcgagagt aaagctgcgg
     3241 ataacgagac aatctttttt caataccagg cataccgata agaaccattg ccagatcatt
     3301 tctatcgtaa atatctctta gctgttctaa atgctgaagc tttaaacgat caatttcatc
     3361 gacaataatt aagtcaattg cttcataggt tttaggcaca ggaggatctt cttgattgta
     3421 ttttagtacc atatgcattg ctttagtcag acctattcta ctagttattg tgttaatatc
     3481 cgaactgaca cgagacatct tttcagctgg tacagtataa aaaactttat ttacacttaa
     3541 tatttcatct gttgcattta tcaagaacaa gaaaaagtct gaatattcaa aaaaaataac
     3601 ggtgtacatt tttaaaagga gatgtataat cgagatttag aaagcgtttt cctcaaaatt
     3661 taatgaatta ggaagtgatt actatgaaaa aattatttag cgcacttttt attctgttat
     3721 ttggctttat tggtattggg acttctggaa atatagttaa agctagcacg atggttgaac
     3781 aaacaagtga aatggcaaaa acttttagtc aattaacacc agaagaacaa agttacttca
     3841 aatccaaagg atttagtgat aaagatactt tttacactca aaagatagag cctcaagatc
     3901 cgaaaacacg tttttctgca aatgtattgc ggttaaccgc atctactaaa aaaattacta
     3961 atacgcaggg gcggacagaa tatattataa caagtcccaa ttcggcgatg tatcgtattt
     4021 ctacacgctt gagtttaggt agtgcaaaat catatactca aacagtaaat ctagctaatt
     4081 cttatggtta tggtggagga atgtatttta catttactgg gaaaagacaa tattacagtg
     4141 taaaattagc tatgcaagtt tataatacat ttggtgtagg gtcagtctct tgcactgcgg
     4201 gaggattgac aataggaaaa tgataatttc ttactatgtc ttaaatagaa gtgatgatga
     4261 aggactcttc gtttttcaat cattacatga ggatacgatg ctaaaagagt tttataatcg
     4321 gattaagaat aaacagatag aaacaaattt atatttttta agtgactcta tcttgccttt
     4381 tattagcaac cctaaaattc ttaacttgtt gcagacaaaa gatttgaaaa gtttaccgat
     4441 tattgtcatt gatgaggaaa ttgtaaaata tggcgcttat ctaagcgtag aagaattaga
     4501 aaaattaact aacattggaa tgagtattca ggaggataat ggggtacatg gaacatagaa
     4561 tgaatatgca cagttgagaa cggaatttga c
//
LOCUS       DACCXW010000167         4581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCXW010000167 DACCXW010000000
VERSION     DACCXW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.175"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(29..1261)
                     /locus_tag="IVY71_002668"
     CDS             complement(29..1261)
                     /locus_tag="IVY71_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7986686.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="IVY71_002669"
     CDS             1516..2085
                     /locus_tag="IVY71_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7986687.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="IVY71_002670"
     CDS             complement(2263..2703)
                     /locus_tag="IVY71_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7986688.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="IVY71_002671"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="IVY71_002671"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7986689.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="IVY71_002672"
     CDS             3657..4580
                     /locus_tag="IVY71_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7986690.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1382 a          854 c          908 g         1437 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag a
//
LOCUS       DACCXW010000168         4423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCXW010000168 DACCXW010000000
VERSION     DACCXW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.176"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(50..1198)
                     /gene="nagA"
                     /locus_tag="IVY71_002673"
     CDS             complement(50..1198)
                     /gene="nagA"
                     /locus_tag="IVY71_002673"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7986691.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1274..2110)
                     /locus_tag="IVY71_002674"
     CDS             complement(1274..2110)
                     /locus_tag="IVY71_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986692.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2294..3511)
                     /locus_tag="IVY71_002675"
     CDS             complement(2294..3511)
                     /locus_tag="IVY71_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7986693.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3894..3977
                     /locus_tag="IVY71_002676"
     tRNA            3894..3977
                     /locus_tag="IVY71_002676"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3928..3930,aa:Leu,seq:gag)
     gene            complement(4217..>4423)
                     /locus_tag="IVY71_002677"
     CDS             complement(4217..>4423)
                     /locus_tag="IVY71_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986694.1"
                     /translation="SRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQ
                     KNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1297 a          939 c          730 g         1457 t
ORIGIN      
        1 cttaaaacca aacttaagaa gtttagtttt aaggttaact taaattatat tatgcgtgga
       61 aacgttccac gccatctaga taagttgctt ccaatgtcat atcagggttc aatacgataa
      121 agtctgcatc gcgtccttga gcgatttttc cacatgtatc atcgatttta cagctgattg
      181 ctggaatcag actagccatc atgactgctt gttctggtgt tgctaatccc cagtctacaa
      241 catttttaat tgcttctttc aatttcaata tacttccagc taagttgcca gattctagac
      301 gtgctgttcc ttcttttact actactggaa attcgcctaa attgtaattt ccatcaggca
      361 tcccaccggc catcatacag tctgtaatta atgcgacatg atcatgtcct gctttttcca
      421 ttaggattcc cgcagcattt ggatgtacat gatggccatc acaaatcaat tcagaaaaga
      481 catgttctaa agataataac gcacccacca tacctggttc gcgatgattc aatccacgca
      541 taccattgaa agcatggaca aatacacttg cgcctgcttc aacagctttt tgcgcttctt
      601 ctagtgtagc attactgtgt cctagagaaa caactactcc ttcttcagtc acttgtttga
      661 caaactcttc tacaccttga cgttccggtg ctaaagcaat ttttttgatt aaaccacctg
      721 aagcttcttg ccattcatgg aaagtatcca aatcaggatc cccgaaataa cttgggtttt
      781 gtgccccttt gtgttcttct gtgaagaaag gaccttcaaa atagattcct ttgattttag
      841 ctcctggtgc ttcctgatac attttgccga ttgtctcagc tacatctttt aaacgttctt
      901 tgcttgacgt caatgttgtt ggtaaaaatg aagtcactcc acaagataac agaccttcag
      961 acatcacttt gatgccttct gcatcattat ccattacatc atgattcata tagccgtgaa
     1021 tatgtgtatc taccaaacca ggagcaatcc atttgccgct ttggtcaatg atgttgactt
     1081 ctccctcagg tagttctttg gtgtattcac caaaaagtcc atcggtaatt tcaagatagc
     1141 ctgcatgttt gacacctgac gctaaaaaga atttgtcagc atagataaaa gttctcattc
     1201 tattctctcc ctttgttttt ctacctctaa agtactctta tttagagaag aagtcaagaa
     1261 tggtctacac catttacaaa ttttttgaaa tttgtttcac agcttgtgct aagccttcat
     1321 agaatgctgc tctttctggt tcttctttac gcttgtatat taagctttta agcacaaata
     1381 aatcattgag gtaatagtag ctattcttct cttgagccca atagattcct tgattcacct
     1441 gttctaacgc ttcgtcgatt tcattttgat ggaataagaa atttccataa ttataaaaaa
     1501 tttgcgaaaa ttcacttttt gatcgttcat cagctgattg atgcagcagt tggatgcttc
     1561 gactcaaata gtagcgtgct tcagccgttt ttcctgccac accatatatt tttccgatac
     1621 aactaatcag ttgtatttct gtgtcagaaa catggattct ttctttttgg ctagagtagc
     1681 ttaatgcttc tttcaaataa taaagggact gagagatatt ttgacgcaac gtgaattcgc
     1741 aaacaccgag ataataataa tagcgttgaa agtcagttgc ttgatacaga ttttcagaaa
     1801 tatcagaaga ttttagtact tgttctaact tccgataatc acgtttttgg aaacaactat
     1861 ccaacagttc aaataattta tcgtttgtcc gagtagtttt tcgagagaga ttcatcacat
     1921 gatcaatcgt tacccctagc cgttgacaaa tctgcatcat caccaataca ttcggcagtt
     1981 cctcattatt ttcgatccga cttaatacac tttgtgaaca aatatcatgc gaaagcattt
     2041 tttgcgtcat tttttgttct tttcgaatct ctttaatgat cgttccaaag gactcgatta
     2101 acgtttccat aagtatcctc cccaaaatat gcgtattcgc attctattcg tattttactt
     2161 taggaagtcg caaacttcaa gaaaagactt gcaactaaat aatatacttt agttatttaa
     2221 gaaaactcca ctagaaagag agcctcagtg tgtttgtctg tggccctcct actgaaaaat
     2281 ttggttttta aaatcatttt tggcgatagg ttgctaggaa ttctctcatt ttcttagctg
     2341 tttgtttcaa atcatccatt ttaggaagat aaacgattcg aaaatgatct ggctggttcc
     2401 aattaaatcc accaccatgt acaagtagaa tatgatgttc atgtaaaaag tccaaaacga
     2461 atttttcatc atccaaaata ttgaatcgct ttgtatcaat ttttgggaat atataaaaag
     2521 cagcttttgg ttttacggct gaaagaccag gaatatcatt gattgcatta tagatatatt
     2581 ctctttgttc atagatcctt ccgcctggca ggagcagctc atcgacactt tgataaccgc
     2641 ctagcgcggt ttggataatt tgctgagaca atacattgga acacaaacgc atggaggaca
     2701 acatattcag cccttctata tagcctttga cgtgtgactt atctccactt aggaccatcc
     2761 agcctactcg aaatcctgct acacgatgag atttggatag accgttcagc gtaacaacga
     2821 acaaatcagg cgctaatgta gcaatcggaa tatgttctag accatccatc actaagcgat
     2881 catagatttc gtcggaataa ataatcaggt tattttgtcg agctacttca acgatttctt
     2941 ctaataattc ttttggataa agtgccccag taggattatt aggattaatg ataacaatag
     3001 cttttgtccg actcgttatt tttgatttga tatcatcaat atcaggatac cattctgcct
     3061 cttcatcgca aatataatga acaggtgtgc ctccagctaa tgcaatagaa gccgtccaca
     3121 acggatagtc cggcattgga accagtactt catctccatt gtccagcaac ccctgcatac
     3181 acatcgtgat caattcgctc actccgttgc cggtgtagat atcattgatc gtcacattcg
     3241 gaaaatgttt cagttggcaa tattgctcaa ttgcttttct agcagaaaag atccccttgg
     3301 aatctgagta gccttctgag tcacgggcat tcatgatcaa atcacgaatg acttcgtttg
     3361 gtgcttcaaa tccaaaaggt gcgggatttc ccgtgttcaa ttttaaaata ctaatgccct
     3421 cttcttgcat ccgctccgct tcttctagaa cgggaccgcg aacatcgtaa ctgacacctt
     3481 ctaatttgtt ggatttttca aattctctca tcacttagcc aatccctcat ttcaaaaagt
     3541 tagaactttc caaatttttt gttatttact atttcaatat catactccat cctagaaaat
     3601 tttgccactt atttaggaaa aacgtaagta ttaaaaaaac acaaacaagc gttgagaact
     3661 taattgtcat gatagtcatt ccatagattc gctgcccaat ccattgcaat tccacaaatg
     3721 attcctagca taaaaccaag caaaaaacgt ccgaatgcaa gtccaatcaa tacacctaag
     3781 atcattccca gtcctgaaaa ttctccgtca tatttctcca tcttaataag tcctcccatt
     3841 tttgagcttg acaactatgt aaaacctcgc tatcattagt gtgttattat tacgcagatg
     3901 tgttggaatt ggcagacaag catgattgag ggtcatgtga gcgttagctc gtgtgggttc
     3961 gagtcccatc atctgcacta caactcgtga agttaatttt gcttcacgag tttttttgtt
     4021 tgaacgtttc tttatgccat tatatacata tcactgtatc ctttacaaaa agaacgtaca
     4081 atcatgtaaa cagcctgagg tgagtatatg aaggtcggaa acaagctact cgtagaaact
     4141 tgcaagtgtt aaaacaatta atcgaaaaac gagtaaaatc cctcacctaa tactaaagcg
     4201 agggattttt tgtatattat ctgtttatca ccgttattat aaaggtacaa ataccaagca
     4261 cggttaatcc aaattgcata aattcatttt tttgtacact catgcttttc attagctctt
     4321 taactgccat tagtactaag atggcgataa caacataaag tatatagtac gtccgcattc
     4381 cggaagccca tactgtggga gaaaatccca taattaacct tga
//
LOCUS       DACCXW010000169         4399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCXW010000169 DACCXW010000000
VERSION     DACCXW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.177"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            156..1862
                     /locus_tag="IVY71_002678"
     CDS             156..1862
                     /locus_tag="IVY71_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7986695.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IVY71_002679"
     CDS             complement(2020..2922)
                     /locus_tag="IVY71_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7986696.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IVY71_002680"
     CDS             complement(2919..3623)
                     /locus_tag="IVY71_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7986697.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IVY71_002681"
     CDS             complement(3654..4295)
                     /locus_tag="IVY71_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7986698.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1348 a          945 c          761 g         1345 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctccctta
//
LOCUS       DACCXW010000170         4369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCXW010000170 DACCXW010000000
VERSION     DACCXW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.178"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            764..>4369
                     /locus_tag="IVY71_002682"
     CDS             764..>4369
                     /locus_tag="IVY71_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HAP7986699.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDIIENPGDNIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVL
                     KAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVTFTAGEG
                     TSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATK
                     LDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKK
                     GTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLP"
BASE COUNT         1644 a          675 c          892 g         1158 t
ORIGIN      
        1 atacatgatg aatttatgga ttatcaaaaa ttggatcagt taattgaaag agaaagctgg
       61 gaagattatt acgtgggtct aactttttca gaaaagttaa acaactacaa gttaatagat
      121 tataagtatt tgagtgaaag caattaatta ttcatgtaga gtactagaca ctttaaatat
      181 ggacaattca attataaaga aaaagaatca ataatttaat gaggctgtga caaatgtcat
      241 tttttgtttc gctaaaaacg agtgaaaaaa tgttgataac tccaatcatt cgtttaagat
      301 tctaggaatc attcagaaat gctgtgaaac cgatacttta taattataaa gaaggtgatt
      361 tcaaattccg aagaaggaat ttgaagtcac ctttttactt atgtcacagt ctcattcagt
      421 aatgcgggat aaggctcctt attccgttat ttgaatactc tgaagtttaa tctaagaaag
      481 ggtaatattt tgaaaacatg cttcatcgca tagaaatgaa cttaatttca gaaagaatac
      541 atacatatct ttttatatgt tttcagctgc tgaatggtta aaaagcacaa atatccgaaa
      601 aaaacatgaa gaaatgtact ttttttgttg taatgttcat gaaattctct aagtaaatta
      661 gttaacataa ggttagtaag attcctattc tgtgaataat acgtatcaga tttatctttt
      721 attcttacta ggaaagtaat tattaaggga gttgatttga ataatggtta gcaagaataa
      781 taagagagta tttctagaaa aaacaaagaa acgagtattg aagtacagta tcaaaaaatt
      841 aagtgttggg gttgcatctg ttttgattgg tgttggtctt gtcttgggaa caactgaact
      901 agttaaagca caagatgaaa tttctccaag cagcccactt gaaacagcga taagctccgt
      961 acaagtaggt gacaaagtag cttcggggaa tacttttcag gagaatcctg gttatacgaa
     1021 aaattataac ttttcggatt tacaatttag tcctcaagaa ctaactggag acacactaaa
     1081 aggcaataca attggatttg aagtttacgg aaaacataat attgcagcat caactaaaaa
     1141 ctgggaaatc cgtcttcaat tagatgaacg attagctaaa tatgtagaaa aaattcaggt
     1201 cgatccaaag aaaggaattg gatcttctag acggaccttt gtaagaatta atgattcgct
     1261 gggtagacct acaaatattt ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc
     1321 tggggcagaa acaaccgata cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag
     1381 cctagatgaa atttttaaag agataggcat agacaatctc aagaccgatc gtttaatgta
     1441 tcgaatctac ttggtaagtc atcaagacga tgataaaatt gtaccgggta ttgatagtac
     1501 gggctacttt ttaacagatt cagatgattt ctataatagc ttagatgtat ctgaaaataa
     1561 tcctgaccag tttaaacatg gatcagtaag tgcaaaatat gagaaaccta atactcaaac
     1621 aaaagatggt tcaggatcca caggtgctaa tggagctata atcttagatc ataagttaac
     1681 gaaaaattat aatttctcat attcatcttc cgcaaagggc acgccttggt atgctaatta
     1741 caaaattgat gaacgattag ttccatatgt agcgggtata cagatgcaca tggttcaggc
     1801 tgacaaagta acatatgatg tttcgtttga atccggaaca aaagtagctg atttagcaat
     1861 cgagcgacgg gaaggtcatg aaaattatgg cataggttca ataactaata atgatttaac
     1921 taaacttatc gattttgcta atgcaagtcc acgtccagtc gttattcgat atgttttaca
     1981 attgactaag ccattagatg aaatcttaga agatatgaaa gcaacagcac aagttgaaga
     2041 aaataaacca tttggtgaag atttcatctt tgattcttgg ttgtcggata cgaataaaaa
     2101 attaatccag aacacttatg gaacaggtta ttattatttg caagatattg atggtgatgg
     2161 aaaccctgac gataaagaag agagcggaga cacgaatcca tatatcggga aacctgaatt
     2221 agaagaagta tatgatgttg acacaacagt taaggggaaa gtattcatcc acgagttagc
     2281 gggaacaggt cacaaagccc aacttgttga taaagaaggt actgtattag cagaaaaaac
     2341 tatcgctcca aatgaaaaag atggggctcc aatttcagat actgtagaat ttgaatttac
     2401 gggtgtagat tcaagtaaat taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc
     2461 aggttttgat aaaccagaag aaggttcaac cgttattaag gaatcaccaa aagcggttga
     2521 taaacaaacc gtggtagttg gatttaaacc agatgctaaa gaatcaattc ggaataataa
     2581 aaacttacct gaagatgcag agtattcatg gaaaacagag cctgatactt ctaacgttac
     2641 tgatagtacg aaaggtattg taactgttaa gatcggaaat cgaactttcg acgtggatgt
     2701 agagtttgct gtaaaagctt ctcaagctat ggaaaatgat gcaacatacg tacctataac
     2761 aacaactcca gaaacgacag ttcaaagtgg aaaacctaca tttgataaac cagatgttcc
     2821 tttagctaaa gatgcctttt caatttcaga tgtctataat aagtacttcg gtaatgcaag
     2881 tgttgatgca aacactggta ttgttacatt cactccagct aaaggtgtag gagaatcgga
     2941 gccgattact ggaacaattc ctattaaaat tgtttaccaa gatggttctg taggcacgac
     3001 cgatttagca gtaactgtaa gtaaagatat ttatgaaaat ccaggagaaa acattcctgc
     3061 aggctaccac aaagtaacct tcaccgcagg ggaaggaaca agtattgaaa gtggaacaac
     3121 agtctttgca gtgaaagacg gcgtaagctt gccagaagat aaacttccag tgttgaaagc
     3181 aaaagatggt tatacagatg cgaaatggcc agaagaagca actcaaccaa ttacagcaga
     3241 tgatacagaa tttgtatcaa gtgcaacaaa actggatgat atcattgaaa atccaggaga
     3301 aaacattcct gcaggctacc acaaagtaac cttcaccgca ggagaaggaa caagtattga
     3361 aagtggaaca acagtctttg cagtgaaaga cggcgtaagc ttgccagaag ataaacttcc
     3421 agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg ccagaagaag caactcaacc
     3481 aattacagca gatgatacag aatttgtatc aagtgcaaca aaattggatg atatcattga
     3541 aaacccaggg gataacattc cggctggcta tcataaagta acctttactg caggagaagg
     3601 aacaagtatt gaaagtggaa caacagtctt tgcagtgaaa gacggcgtaa gcttaccaga
     3661 agataaactt ccagtgttga aagcaaaaga tggttataca gatgcgaaat ggccagaaga
     3721 agcaactcaa ccaattacag cagatgatac agaatttgta tcaagtgcaa caaaactgga
     3781 tgatatcatt gaaaatccag gagaaaacat tcctgcaggc taccacaaag taaccttcac
     3841 cgcaggagaa ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt
     3901 aagcttacca gaagataaac ttccagtgtt gaaagcaaaa gatggttata cagatgcgaa
     3961 atggccagag gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc
     4021 aacaaaactg gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac
     4081 tacagaattg aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc
     4141 aaaagatact aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa
     4201 agggactgtt gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac
     4261 agtgacagac aatcgttctg acgccgataa atatgagcca acagtagaag gcgaaaaagt
     4321 agaagttgga ggcacagtag atctaacaga taatgtaacc aacctacca
//
LOCUS       DACCXW010000171         4355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCXW010000171 DACCXW010000000
VERSION     DACCXW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.179"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(21..917)
                     /locus_tag="IVY71_002683"
     CDS             complement(21..917)
                     /locus_tag="IVY71_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7986700.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(910..1590)
                     /locus_tag="IVY71_002684"
     CDS             complement(910..1590)
                     /locus_tag="IVY71_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7986701.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1591..2373)
                     /locus_tag="IVY71_002685"
     CDS             complement(1591..2373)
                     /locus_tag="IVY71_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7986702.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2366..3283)
                     /locus_tag="IVY71_002686"
     CDS             complement(2366..3283)
                     /locus_tag="IVY71_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7986703.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3447..3770)
                     /locus_tag="IVY71_002687"
     CDS             complement(3447..3770)
                     /locus_tag="IVY71_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7986704.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1278 a          948 c          759 g         1370 t
ORIGIN      
        1 ttctcactaa tcttgtataa ttaaaccctc cgcaacttga cggagatgga gaagatgccc
       61 ccgtctaatc tagcggtcat ctctcctccc attccttcca ccaatttttt tgacaggtaa
      121 agcccgattc cgctagattc cgtatttgtg cggctggtat tttcggtata aaaacgctgg
      181 aagacttttt caacagaggt ttgcgttcct ggtttgatag gattggccac tgttaatcga
      241 atgaatggat cctcttttga aaatgtcacc tttgcttgtc catctgcata acgcaataaa
      301 ttctgtatga tattttgcag cactcgcagc aacatgagcg gatctgctaa tacagttgta
      361 tttatcgagt gttcagcgaa cgctacctcg atatttttct cagaaaattg ttcataaaaa
      421 gagaaaaata cttcttccac ctctttagaa aaagaaagcg tatggagatc catttctttt
      481 tgcttcgtct ctattcgagc aagatcataa aactgatccg tcatactgag taatcttgca
      541 acagattgtt tgatgatcgt taaatacttc tcccgtttct ccgtatcctc tacctgttcc
      601 agtaattgta cataaccgtt aatagcagtc aaaggagttc tgagatcatg agaaaggccg
      661 acgatcgata gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt ttgttcattt
      721 tcatccaacc atgtattcag tgcatctact aggtccatta gattcttttc tcgaaattcg
      781 actgaaagac gaccaccata ttcttgtcgt tcagaaagag tggcgatttt cttagttaat
      841 ctcttgattt ccctttgcag gaagaaaata aaacttccca taaagataac aacgagaagc
      901 aacagccatt taatcaaatc taaaccccac tccccacacc gtatgaatat acacagcatg
      961 tgcctttgct agcttccctc gaagattgga aatatgcata ttcactgttt tctcactatc
     1021 aaaatatggt tcttgccaaa ctgtttcata aagatttgct cgcgaaaata cttttttcgg
     1081 atgttctaaa aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga cagccacacc
     1141 attcacattt acatccctcg actctttgtc cagtaagatt tccttgtaat agatgcgttg
     1201 cgacagatct tttacttgga cagaagtctg atgacgaagt tggatgccga ttcgtaacaa
     1261 aagttcttcg atatcaaatg gtttcgtcac atagtcatcc gctcctgctg ccaacaagga
     1321 aactttatct ctttgatcgc tttttgctga aagaacgatg atcggaacat tcgatgtctg
     1381 tcggatttct gctaacaatg cttcacctga aagccctgga agcatgagat ctagaaggat
     1441 caaatcgaca ggatatgtgc ttaacaataa ttttccttcc gtacctgaat aagcatcaag
     1501 aagtttgtat ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt catcgtcttc
     1561 gataatcaaa atagtttcca gcaggaccac ctacttaatg tcagattttt ggaaaataaa
     1621 cattcctaga gctgatgata ccacgattgt tactagactg accagaatca ttttcggtgc
     1681 ttgcgaataa gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca
     1741 ctccgttaga aattccaagc ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc
     1801 catgatataa agaaaagaaa gaagcaaact gacactggta cttctagtga tgaatgtcaa
     1861 agccactgga acagataatg tccctaatgt cagcagaagc aatacaggaa atacttttag
     1921 aacataattt ccatagtttt tgattccctc aacttcacca aatccgaaag caagtgtgta
     1981 agcaagcatg gctagtcccg tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac
     2041 gagaagtttc gacacataat aagtcattcg attccgtcct aaagataacg tattgcgaat
     2101 cgtaccgttt tgaaattctt ccccaatata aaagcacgca aatgctgcga aagccagaag
     2161 aatccatgca gccatttttt ctaccattaa aatccctgtt gcaccgttga tgactgtatc
     2221 cgttgcttgg tttaattcat tgccgtattt tgtatgcata taccattcgt tccaagaata
     2281 aaaaagcgga agtacgacca tcagtcctag tgagacccac attttacggc tcttccatac
     2341 ttttattttt tcagcagtta atagattaaa catgtggatt gcctccagtt aattgtaaga
     2401 aataatcttc cagtttttgc ccaactaggt gaataccatc cacaggaact tttgcttcta
     2461 ccaatgcttg attgatggct gctacttggt caattccttc aaaaagctgg ataacacggc
     2521 tagattgtac aaaataatcc ctataaccaa gagagtcgag aaccgttact gcttgttccg
     2581 tcgccgaagt gtcaattttt ataaattgac gagattcttg tgccaattct tctttcgata
     2641 attctttgat caagtgtcct tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt
     2701 catctagcaa gtggctagaa agcaagacgg tgatgccccg ttcttttacc aaacgttgga
     2761 tgatttctcg catttctgcc atccctgctg gatccatccc attcgtcggt tcatccaaaa
     2821 tcaaaaattc tggattcgtg atcagtgcat ttgcaatagc taaacgctgg cgcataccta
     2881 aagagaaatt ctttgctttc tttttccctg tattttccaa tcgcatcatt ttcagcaggt
     2941 cttcgatttc tctatccgac acaccgccgt tcgctgcttg gatacgtagg ttatctcttg
     3001 cagttaattc tggataaaga gcaggggttt caatcgattg acccatacgt cgtcttgctg
     3061 cctgcaaatc cgtcgttcca aataattcga tcgaaccttc atcaatcgag atcaaaccca
     3121 taatagttct cataaaggta gacttacccg ctccattttc cccaatcaat ccatagatca
     3181 ttccacgttt gatttcgatt gatactttat ctaatgcttt ggcagcaccg tattttttgg
     3241 ttataccagt tgctttcaag accgtttcat ttccaaaatc catacttatc cttctttctt
     3301 attgatgagt gaagtatagt tgacgggtat aaagaaagca gccagaaatg tataaagaaa
     3361 gtgtaaattt tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt
     3421 atcaggcagt ttgttatacg cttgtatcag tttcctgatt ctttcttttc gaccaaagtc
     3481 gtttctctgt attcctcttc actgaaaatt tttgcttctt tcagatcaag cttgagtaat
     3541 tttaccagac cggttataag gataactata gcaagactcc ctcccagaaa aattagactg
     3601 tctcgcagac gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag tgctaatcca
     3661 agtggactgg ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc
     3721 gttggaactt ctcgctgaag ccagaccata taaaaagcaa aagaaaacat taatccttaa
     3781 aaacggactt atgaaaaata atacatgccg tttgggattt acaattagct accaaataag
     3841 aggttatgac aaaagacttt tcacaatccc ttcttccgaa taaacggtgt tctcctgcca
     3901 actcctcggt cacaagtcac aatatttatc aaacatgaga agcaattttt gtaaattcct
     3961 tcttatttct tgaagctgac cacttctgtc acaacctcag aagcggataa ataatattgt
     4021 ttgatgatac tttatctttt tcccgtttgt ttatagatcg aatgatatta tagaccaaga
     4081 atcctaccta ttaacatagc taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg
     4141 ccagaaaagt taggattaag gtaactgcgc tcagcattgc ttcgcccagt acgattctcg
     4201 taactcgtga actcgaacga taatcgcaat taacgttttg agaactgtga agctttacgg
     4261 gctttcttaa gacctggttt tttcttcgta tcattgaata agtttgaaaa accttgattt
     4321 ataaaggttt tcccgtaaat atattttatt atatt
//
LOCUS       DACCXW010000172         4321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCXW010000172 DACCXW010000000
VERSION     DACCXW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.180"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..598
                     /locus_tag="IVY71_002688"
     CDS             <1..598
                     /locus_tag="IVY71_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986705.1"
                     /translation="FLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEILALTWDNINF
                     KTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLEQRKYLFQLGF
                     TKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASMLYEAGADHKE
                     VQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(691..984)
                     /gene="rpmA"
                     /locus_tag="IVY71_002689"
     CDS             complement(691..984)
                     /gene="rpmA"
                     /locus_tag="IVY71_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7986706.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(997..1341)
                     /locus_tag="IVY71_002690"
     CDS             complement(997..1341)
                     /locus_tag="IVY71_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7986707.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(1368..1676)
                     /gene="rplU"
                     /locus_tag="IVY71_002691"
     CDS             complement(1368..1676)
                     /gene="rplU"
                     /locus_tag="IVY71_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7986708.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            1958..2599
                     /locus_tag="IVY71_002692"
     CDS             1958..2599
                     /locus_tag="IVY71_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7986709.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2661..4292)
                     /locus_tag="IVY71_002693"
     CDS             complement(2661..4292)
                     /locus_tag="IVY71_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7986710.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         1395 a          926 c          750 g         1250 t
ORIGIN      
        1 atttctaaat gaagtatctc aagaacagaa tccatatttt aaaaacagag actatacgct
       61 ctttagacta ttagcattca gcggatgtag aatcggcgaa atattagcac tcacttggga
      121 caatattaat tttaaaacaa atgaaatggc cattaaaaaa actgtagctc gttcagataa
      181 atattatata tctgaaactc ctaaaaccaa aaaatcaaat cgaataattt atttagatga
      241 aaaaactata aagcaactaa aattttggaa gctcgaacaa agaaagtact tatttcaatt
      301 aggatttact aaagctaatt atttgtttac caatgacgaa aataatttca caattaatca
      361 gtcagtggca gaaagataca atatatatcg tgagcgtgcc ggcttacctt atatcggtct
      421 gcatggtttt agacatacac atgcatcaat gctatatgag gcaggcgcag atcacaaaga
      481 agtccaagaa agaatgggcc acgcaaatat aaaaactact atggacacat atacacacat
      541 tactaacagc aaaaaagaag aaacaacaca aaaactaaca aattatatta acttctaaga
      601 aagtatggtc aaaagtatgg tcaaaaaaaa tcccataatt tacaaaaaag ctccatccct
      661 tgctacacaa gagatagagc agtttattta ttaagcttct tgagctactg gataaacaga
      721 cacttgtttt ttgttacggc ctttacgttc gaaacgtact acgccatcaa cttttgcaaa
      781 taaagtgtcg tctccaccga tacccacgtt tacacctgga tagatttttg taccgcgttg
      841 acggtaaaga attgatccac ctgtaactgt ttgtccatca gcacgtttag cacctagacg
      901 ttttgattct gagtcacggc cgttagatgt agaaccgcca ccttttttgt gggcaaataa
      961 ttgtagattc atagataaca acatggtctg cacctcctat ttatcattaa ttgttttaat
     1021 ttggatatat tctgagtttt cagcttctat cgattgtaat cctaataaaa gattttctaa
     1081 tagaatctca gcaatattgt tttgttcttg tgtcattcct gaagtgactt cggtataaag
     1141 ataaccgcct tcttcttcat tcatttcaac aatcggctca aaaccggcta atgaggcaat
     1201 cccgttgaca gtgctgatcg ctaacgcaga aacacccgca caaacgatat cacttccata
     1261 aggaccagca tcagcatgcc cggtcaaagt gaatgaaacg atctgacctg cgtcatttcg
     1321 cttaaatgtt cctttgatca tatataggcc ttctttctca aacgctatta agcgttgatc
     1381 gcgtcgataa ccacttttgt atatggttga cgatgacctt gtttacggtg tgaatgtttt
     1441 ttaggtttgt atttgaaagt aacgactttc ttttgtttgc cgtgtttttc aacagtacct
     1501 tcaacagttg cacctgcaac agttggagcg cctacttttg tagattctcc gcctactaag
     1561 ataacttcgt caaaaacaac tttttctcct gcttcaacgt caagtttttc aacatagatt
     1621 gcttgtccca cttcaacttt tacttgttta ccaccagttt taataattgc gtacatgcta
     1681 taaagcacct ccttctgata tacttagact cgccatccga agtgacagca tatgctgtgc
     1741 ttattacttg ttctgagcgg ttgtagctgt ggagtccaca attacaacag tactagctta
     1801 ccagaacagc aaccgttagt caactaaatt taaaatcaaa aagtaaaaca aaaattcaag
     1861 caatacttct gcttttctat cctcacagtt ctaccaaata aaatagtcgc attgtgtttc
     1921 ttttattctt ataataaaag aaaaaggagg agcataaatg gaatacccaa aagaactgat
     1981 acaagaagct tctttaagaa tgaacggccg tcctgtagaa ggatttatcg ctgggcaagg
     2041 acctcttcat cctaaactga tgctcgtggg agaagcccca gggaaaacag agattgatac
     2101 gcatgttcct tttagcggtc aagctggaaa agaattgatg caggcacttt cttcaactgg
     2161 cttaacacga gaagaggtgt atatcaccag tgctgtaagg agccgcccgt atcgcgtgac
     2221 tcatcggatc aataaacgga cacaacagtc agaaactgtt tatccaaatc ggacacctac
     2281 tcgttccgaa gtatttgcgc atgcaccaat attggattac gaactgatga aggtccagcc
     2341 aaaattaatt gcgacacttg gaaacattgg tcttcaacgt ttacttggca aagaatatac
     2401 ggttaccaaa aatcatggtc aactctatac agggcctgtc ttacaattaa cagaacaaaa
     2461 ggatgcctac gaatggtcag aaaaaaacta tcgaattctt ccactttttc atccagcagc
     2521 tattttctac aatcgctccc ttgtacctgt tatccatcaa gactggcaag tactagcttc
     2581 acttttacag caatattaaa aagaacagac aagaccataa aatcttcagt ttacagtctt
     2641 gtctgttctt tttcattgtt ttaatggcta attgcagtat atttcgcatg taaagctgtc
     2701 aacgcatgag caactgtttg atgttgatgc gtgaatgtat gttcccacaa gtgagaaagt
     2761 tgtttttcta cttctggctg caaggaagta tagataatcg tttgtcctct ttgctctgct
     2821 ttttcttgaa attcatcaaa aactgcacgt gccgtcaaat caatacttgg tacgcctcgc
     2881 atggagaaaa tgacaccatc gtgatgattt agttcttcca atttattttt gagtttttct
     2941 gaagacataa aaaacagtgg accactaata tacactactg tccagttatt cagcttttcc
     3001 gtttctggca gccccattct ttgccaatcg atcgtttcta atgaaatcgt gatagcagcg
     3061 cttttgacaa caaaaaatac acagccgccg atcaccccga tcacgatagc aatacttaaa
     3121 tcaaagataa ctgtacaaat catcgtaagg ataaacaata gaactgctga ccaatattta
     3181 ttagtaaata gctccttgat cgtttcccat tcattcatac gccaagccgt cacaatcaaa
     3241 acacctgcaa gtgctggcat tggaatctgt gacatcactg gcgcaaaaac aagcatggag
     3301 agcagtaaga caactgcatg tatcatacca gctaatctag tttgtgcacc agatttgatg
     3361 gccacgcttg tacgggcgat ggccgcagta gcaggaatcc cgccaaagaa aggaagcaac
     3421 agatttccaa ttccttgcgc cattaattct tgattgctat ctaagggctt atttgccatt
     3481 cggccagccg aagcaccaca tagcaagctt tcaatcattc ctaataaagc aatgctaata
     3541 gctggaactg ccacttgttg caaggcagaa aagctaaaat ctcccatatt caaacgatta
     3601 ctactgatca atgtttgtgg aatcttaccc acagttgcta ctggtaaatg gaacaatagc
     3661 ataactgctg ttgccaaaat gatagccagc agcgaactag gcattcgttt cgcccatttt
     3721 tttggataga taaacatccc taaaatgact agccctccca tgatcaaaga ggctgtgttt
     3781 atcggaaatc ctatcgtttg ataactactt agtttttcaa taacgtttgt accttccgag
     3841 tgaacaccaa ataaattatc gatctgacct aatgcaataa tgatagcaat acctgaagta
     3901 aaccccgtaa ttacgggtgc tgggataaaa gaagtcagtg ttcctaatcg gaaaagtcct
     3961 gctgcaagta aaaatagacc cgctaaaaaa gtagcaagca atacaccttg cattcccttt
     4021 gttgccgcaa tagacatcaa gatagctgcc atcgcacctg tcggacctga tatttgataa
     4081 aaaccgcctg ataaaccgcc aataacgata ccggcaatga tagccgtaat caaacctgcg
     4141 gccgcatcag ctccgcttga taccccaaaa gccaaggcta aaggcaatgc tacagcagct
     4201 actgtgatcc cagccaaaag atctttctga aatcttgaaa gatcataacc tgaaaattct
     4261 ttttttagtg aggaaacata atttttgaac acctatctaa ctcctttttc atgaaatttt
     4321 c
//
LOCUS       DACCXW010000173         4205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCXW010000173 DACCXW010000000
VERSION     DACCXW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.181"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            75..503
                     /locus_tag="IVY71_002694"
     CDS             75..503
                     /locus_tag="IVY71_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7986711.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="IVY71_002695"
     CDS             490..732
                     /locus_tag="IVY71_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986712.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="IVY71_002696"
     CDS             complement(1032..1247)
                     /locus_tag="IVY71_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986713.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="IVY71_002697"
     CDS             1354..1668
                     /locus_tag="IVY71_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986714.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="IVY71_002698"
     CDS             1772..2950
                     /locus_tag="IVY71_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986715.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="IVY71_002699"
     CDS             3353..3646
                     /locus_tag="IVY71_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7986716.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3734..3847)
                     /locus_tag="IVY71_002700"
     CDS             complement(3734..3847)
                     /locus_tag="IVY71_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HAP7986717.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
BASE COUNT         1441 a          627 c          817 g         1320 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaa
//
LOCUS       DACCXW010000174         4118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCXW010000174 DACCXW010000000
VERSION     DACCXW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.182"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(111..341)
                     /locus_tag="IVY71_002701"
     CDS             complement(111..341)
                     /locus_tag="IVY71_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP7986718.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(571..1389)
                     /locus_tag="IVY71_002702"
     CDS             complement(571..1389)
                     /locus_tag="IVY71_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7986719.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1550..2239
                     /locus_tag="IVY71_002703"
     CDS             1550..2239
                     /locus_tag="IVY71_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7986720.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2253..3755
                     /locus_tag="IVY71_002704"
     CDS             2253..3755
                     /locus_tag="IVY71_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7986721.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3768..>4118
                     /locus_tag="IVY71_002705"
     CDS             3768..>4118
                     /locus_tag="IVY71_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7986722.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDK"
BASE COUNT         1347 a          608 c          737 g         1426 t
ORIGIN      
        1 aaaggatcat aaacttttaa caagtgatga aatgggtgga aattgattct tatagacagt
       61 ttgtctcata acattgaagt attttgcaat tttagcattg aaacactttt ttaatcttca
      121 acgttgttct tataaacaag atgattggga atcttttgaa ttaacactgt atagcttaga
      181 acacccttta tagaaaacat tcgcttagca atcttaataa ttccttgtgc ccgatgtcgt
      241 ttagattcag taggttcctg ctcatcgatc atggaaaaaa tttgaaggat cagatcctta
      301 ataaatttat ttaagagagg atgatccatt gcttcatcca tactcgataa actagtgata
      361 atgagctaca cgtttttctt tcaagttgat caatagcttc cacgataaaa atatctttag
      421 ataaaatcaa gaaccgtagg aactacaggg atatttggtc aataagagcc accgctatta
      481 gtaaaaaata agctatcaag tatgctctca tcccaagaat tgcctacttc aatcgttaaa
      541 aaccgttagg tttagctaag tgggggtagt tcactcaata cttttatcaa taatagagcg
      601 caaaactttt tctcttaatt caactgaatc aatattattg ttttgttcat aaccatgaac
      661 taatagatca caaatataaa gttgtgatat ttttccagct aaagatccac cattaagaaa
      721 ttcttcaatg gctgtttgta aaactaagtc tgcagacttt cctataggtg aatgaatata
      781 gttagttata gcaataattt ttgcgccatt ttttttagct attttcaatg aatcataagt
      841 atctttcgtt ttcccagata aagaaaaaat gattactaaa tcattaactg ttaataaaga
      901 agcgacttgg gcttgaaaat gagggtctag tacagctttt gcttgaactc ctacgcgaag
      961 aaacatattt tctaaatcaa gactagtatt tccactagat cccactccaa atatatatat
     1021 gttttttgat tgagtgatca ttttaatggc ttcattcaat ttttcttcgt ctaaaagcct
     1081 aatagtggaa tagagagctt ctgttaaact attagcaatt tcatcatagt actttacact
     1141 agtcggatgt tctttttttt gactaaagtc ttcctttgca atttcaattt ttaaatcaga
     1201 aaaaccagaa aaaccaattt tttgacaaaa gcgaataata gtagcatcgc caacttttgt
     1261 gtttttctta atatcactca tcgtactata aataatagtt ttcccagaat ttaaaataaa
     1321 atcagcgact tttttttcag acttcgttaa aaatggataa ttttctttaa ttaaatagat
     1381 ataactcata caaactcctc tacaatttca acttttctcc attatactcc aaaataataa
     1441 aaaaaactcc taaatactcc atttttatct ttacttttat gaaagcggat gctatattgt
     1501 ttttggagtt gttatccaaa aataataaaa aattggagtg tttaggataa tggataaaaa
     1561 gaggttttta gatcttattt caaaaggttt gattgtttcc tgtcaagcat tacctggtga
     1621 accattttac aaagaggaag aaagtttaat gccttatttt gctttggctg ctcaaagggc
     1681 tggagcagtt ggtatacgtg caaattctgt tcgagatatt attgaaatta gaaagtgtgt
     1741 atcattacca atgattggaa ttattaaaag agattatcca ccagaatctc cattcattac
     1801 tgccactatg agagaagtag atgaattagt agaaaccggt gttgaggtaa tcgctcttga
     1861 ttgcactctt agaaatcgac atgatggact atctataaat tcttttatta ctaaaataaa
     1921 agaaaaatat cctcatcagt tatttatggc agatatttct acatttgaag aaggcaaaaa
     1981 tgcatataaa gcaggaatag attttgtagg aactacttta agtggatata ctgaggaaag
     2041 taaaaaacaa gatgggccag atatagattt gattaaaaaa ttggtagaag aagggatttg
     2101 tgtaattgct gagggaagga ttcatactcc agaacaagca aaagagataa aaaaaatagg
     2161 agttgcaggt attgttgtgg gtggtgctat tactcgacca caggaaattg catcaagatt
     2221 tgttaaagca ttaagttagg gggagaaata aaatgtttaa aaaattttca caattaggac
     2281 gcgctttcat gttaccaatt gctatattac cagtagccgg attactattg ggactgggag
     2341 gagcgttaac aaatgagagt gctataaatg cttatccatt tttagatcaa ccgtggttgc
     2401 atacaatttt aagtattatg agttatgctg gaaatgcagt ttttgcaaac ctagcactaa
     2461 tttttgcaat tggtattgct gtaggacttg caaatggaga taaaggaaca gctggtttag
     2521 ctggtggagt agcttatcta gtatatactg caactattag tgggtttctt gaactttttt
     2581 cagcaaaaga tgcaacacta gatacaggtg tagtgggatc aattgttgtt ggtggtactg
     2641 ttgcattttt acacaatcgt tatagaaaaa ttgaattacc acaattctta ggtttttttg
     2701 gaggatcaag atttattccg attgtttcta gtttagctgc aatcattatt ggttctttct
     2761 tttatttaat ttggccacca attcaaaatg gtttagtagt tgctggtgaa caaattgccc
     2821 aaatgggtag tttaggaact tttttatatg gttttttatt gcgtttaaca ggtgctgttg
     2881 gactacacca caccatttac cctttgttct ggtatacatc tttaggtggt accgaaactg
     2941 tagctggatc aacaattgct ggagcccaaa atattttctt tgcccaacta gctgatccaa
     3001 accacactgg tctgtttact tatggtacta gattttttgc aggaagattt gcaacaatga
     3061 tgtttggatt accagcagca tgttatgcaa tgtatcgtgc aattcctaag aaaaatcgta
     3121 aaaagaatgg tgggctttat tttagcgggg cccttacatc atttttaact ggaattactg
     3181 aaccagttga atatatgttt ttatttgttg ctccttggct ttatgtgatt catgcattct
     3241 tagatggatt atctttttat tttgctgata tcttaaatat tcgtgtaggg aattcctttt
     3301 caggtggttt gatcgacttt ttattattcg gtgtacttca agggaatgac aaaactaatt
     3361 ggttaaaatt aattcctttt ggtattgcat gggcatttat atatttcttt gtttttacat
     3421 tctttatcaa aaaatttaag gtagctattc ctggaatgga agaagaatca gaagttcatt
     3481 ctgaaaaagt aataaacaaa aatacatcat tacatgaaga gtctcttatc attattgaag
     3541 ctcttggtgg aaaagaaaat attgagtctg ttgaagcttg tgcaactcgt ttgcgtgttg
     3601 ctgttacgaa tggggagatt attcaaaaac aaacaataaa agacactggt gcaactgctg
     3661 tcttcgaagt gaagggtggc atccaagctg tttttggagg gaaagctgac ttattaagtc
     3721 aagaaatcaa tcaaatatta ggaaaagata attaggaggt ttttaatatg tttgaaattt
     3781 tcaaaaagaa aaaacaagaa actttttatg caccttgttc cggaaaaatc atttccatca
     3841 cagaagtaaa tgatcctgtc ttttcacaaa aattactagg tgatggtttt gccgtcatac
     3901 ctgatggttc aaaaatttat gctccattgg ctggtcgagt aacaaatata tttcctacta
     3961 aacatgctat taatataaaa agcaactcag gcattgatta tttgattcat attggaattg
     4021 atacagttga attacaagga gagccttttt cgatttgtgt tcaagaaaat gatgaggtta
     4081 ctcctgaaac ctcgttggtt gatgtggatt ttgacaaa
//
LOCUS       DACCXW010000175         4116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCXW010000175 DACCXW010000000
VERSION     DACCXW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.183"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            48..1811
                     /locus_tag="IVY71_002706"
     CDS             48..1811
                     /locus_tag="IVY71_002706"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7986723.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            1895..2353
                     /locus_tag="IVY71_002707"
     CDS             1895..2353
                     /locus_tag="IVY71_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7986724.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            2462..3145
                     /locus_tag="IVY71_002708"
     CDS             2462..3145
                     /locus_tag="IVY71_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7986725.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3292..3906
                     /locus_tag="IVY71_002709"
     CDS             3292..3906
                     /locus_tag="IVY71_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7986726.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            3922..>4116
                     /locus_tag="IVY71_002710"
     CDS             3922..>4116
                     /locus_tag="IVY71_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986727.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         1453 a          672 c          872 g         1119 t
ORIGIN      
        1 aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg agtacagtat
       61 tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct gttgaggaaa
      121 tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc gatgtaggtg
      181 ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat tatgtatttg
      241 attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt cataagcttt
      301 taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc ggatattcca
      361 accaacatga aagtaaacat gactatttta cagtggggca cacatctaca tctatcagct
      421 tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat gtggtagcat
      481 ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac aatggtggtg
      541 ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt gatgagaatc
      601 atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa agcagcaata
      661 acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac gatctggaaa
      721 cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta cttcacgtcc
      781 atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa tatcattggc
      841 atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga gaaacattta
      901 atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct cctattgccg
      961 cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa aaacatccag
     1021 atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct tctgctttgg
     1081 cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa cgggcatatg
     1141 atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta gtcggtggcg
     1201 gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct tacttatcaa
     1261 gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc tctatgctag
     1321 attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca catggtgtag
     1381 aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca gtaaataatg
     1441 gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa gaaacagtaa
     1501 aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg atcacagaaa
     1561 atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc acattagaag
     1621 atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt gattcttcaa
     1681 tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct gtagggcagc
     1741 tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg cgtgtcttga
     1801 atattcaata attcattatg ctcaccatcc agataattgt ttatactagt atctggatgg
     1861 ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag aagaatatgt
     1921 caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt tcttttcatt
     1981 tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa agataaagac
     2041 tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac caaaagttgg
     2101 tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca aaaatataaa
     2161 tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag aaggagaagg
     2221 ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac atgcttataa
     2281 agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag aatttgaagt
     2341 aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt ctcctgaata
     2401 gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg gaggagatat
     2461 tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag ctttggaaaa
     2521 cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa aagaaggcgg
     2581 agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc gaatcgttga
     2641 tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt ttattactgc
     2701 gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg cactggatgc
     2761 aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa tgattcacga
     2821 acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc aagcgcaaga
     2881 agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt cacgaaagac
     2941 gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata tcccagtaat
     3001 tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat taggtgcttt
     3061 ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa gatatgtcga
     3121 agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg acaaaaaaga
     3181 aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt acttttcatt
     3241 ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac catgtttgaa
     3301 cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg ttcagtcatc
     3361 cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa tatcgcaccc
     3421 ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc gatccagaga
     3481 aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga agctgttatc
     3541 catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa tctgcctaat
     3601 gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat catttcagta
     3661 ccagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca tgtcgagata
     3721 cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa atatcattta
     3781 aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt gaatgctgta
     3841 agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat tgcacgtccc
     3901 gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac agattcaaaa
     3961 gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta cttagaagtt
     4021 ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa ggaaatagaa
     4081 atgccagatc aaacgattag cggattgttt aattac
//
LOCUS       DACCXW010000176         4114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCXW010000176 DACCXW010000000
VERSION     DACCXW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.184"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(153..1658)
                     /locus_tag="IVY71_002711"
     CDS             complement(153..1658)
                     /locus_tag="IVY71_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7986728.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="IVY71_002712"
     CDS             complement(1655..2341)
                     /locus_tag="IVY71_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7986729.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="IVY71_002713"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="IVY71_002713"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7986730.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1133 a          866 c          704 g         1411 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa agggatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat attttggctc tataatttat gggactgatg aatcagaatt
     4081 gattcttcag ccccattttt attttaggta ttaa
//
LOCUS       DACCXW010000177         4067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCXW010000177 DACCXW010000000
VERSION     DACCXW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.185"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            86..1435
                     /locus_tag="IVY71_002714"
     CDS             86..1435
                     /locus_tag="IVY71_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7986731.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="IVY71_002715"
     CDS             complement(1563..2153)
                     /locus_tag="IVY71_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7986732.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="IVY71_002716"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="IVY71_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7986733.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1173 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaa
//
LOCUS       DACCXW010000178         4054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCXW010000178 DACCXW010000000
VERSION     DACCXW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.186"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(122..1894)
                     /locus_tag="IVY71_002717"
     CDS             complement(122..1894)
                     /locus_tag="IVY71_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986734.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1848..3602)
                     /locus_tag="IVY71_002718"
     CDS             complement(1848..3602)
                     /locus_tag="IVY71_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7986735.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3712..>4054)
                     /locus_tag="IVY71_002719"
     CDS             complement(3712..>4054)
                     /locus_tag="IVY71_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP7986736.1"
                     /translation="MGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLG
                     SAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVL
                     IGRKVLKKYKK"
BASE COUNT         1348 a          773 c          571 g         1362 t
ORIGIN      
        1 attcaatcat actgattctt tcgtgtattc aaaattagat gctgtttaat aaaattattc
       61 tttttgaaaa agaattataa ctatatccca aaaattgtag gttaatttaa agcctatttc
      121 atcatcgtga ttgagcttct ttttcataga cgaatttctc agatagttta gttttcttta
      181 tgagattttt gtatatttct gaccttcgat acaactcatt atgagtacct tgatcaacta
      241 gttttccttg atccatcact acaattttat ttaccttttc aatagacttc aagcgatgcg
      301 atataattag tactgtctta tcttgtatta aacgatttaa actttcctga atacgttttt
      361 cattttccgc atctaaactg gcagtaattt catctaaaat taaaattggc gcatttttca
      421 aaaaagcccg tgctatggat aggcgttgtc gctctcctcc agaaagtgaa cttccgtttt
      481 ctccgaccaa tgtatgataa ccgtcaggta gtttttcaat aaagtcttcg cagttagcta
      541 aacgagctgc ttttttcact tcctcatctg acgcttgact atttccaata cgtatatttt
      601 ccaaaatact tgtattaaac aaagttacct cttgaaaaac aatagcgatt ttgctaaaca
      661 aagaagctgg agatacacgt ttaatatcat acccatcaat taaaatacag ccttcatcat
      721 aatcatacag tctagacact aatttcaaga tactagtttt cccggaacca ctagcaccta
      781 ctaacgcagt cacttctcct tgtttagccg taaaagagat atgatctaaa attggggtat
      841 tgttatcata tgagaaagag acatctcgca actcaatgtc aaaagatttc agaggactat
      901 ctgatccctc ttggatactg gtttccttca tagctctaat tcgctgtatt ttaggagcta
      961 aataaaatat ttcaagcact gcttctttca tagaatcgaa ggaatccttt atttttattg
     1021 cggctagtaa gtaaccaacg acgtagagta tagtcacttc acccgttaac agtaagtgaa
     1081 cacctactag aatgactact gctaaactaa catatgagaa aatagaggat aaggccatga
     1141 ctgaaaaagt acttaattct acctttaaat gaatcctttc actctcttcc atttttttga
     1201 aaagtctgtc ttgaactttt ttagatagat taaagctatt aatctcttgc tgcaattcta
     1261 tagtttcttg aaattcttcc gagttctctc tcaaagtatc gtaatattta gtattggctt
     1321 tcgtttggga tttctttgat aacaagatta agacaaaact aaataacgtt ggcaaaataa
     1381 cagctaatcc cattttgaca ttaccaacca aaagcatcac tgaaataaaa gggaaaaaca
     1441 gagccatacc gccggcttta ggtattgcat gactcatcgc atgctcaata ccttcaacgt
     1501 cagacatgat agtttgagat aaatctgata aattatgttt tgaaaaatag gataatggta
     1561 aatttgacaa atcgtctgca atttgcactc ttaaatgcgc actttcttta taagtactat
     1621 tatataagtt ttcatactcc ctatctaaca accaatacat gaaaagcaaa gtaacaaacg
     1681 atacggcaaa ataaagccaa cggggctttg tattttctaa aactaattcg tctatcagca
     1741 tcattataat gaatgctggt accatattaa tgcagtatac aaaaaaacta gaaatagaag
     1801 ctttagttaa agcttctgcg ccctgatcac ttaaagcaaa tttgttttta taaaaacttt
     1861 tcattgtcaa gcctccaatc atttgcattc tcataattag tgaccaattg tttatacact
     1921 tgatgtttag ccattaattc attatgattt ccacgttcta ctattttccc ttctgacaag
     1981 acaagaattt catctacaga tttaatgctg gataatcgat gagctatcat aataactgtt
     2041 ttttctttca tcagatgttg aaaagccttt tgtagttcat actcattatc tggatcaata
     2101 gatgcactgg cttcatccat aataacaatc tttgcatctt ttaaaattgc tctggcaata
     2161 gctatacgtt gtttttctcc tcctgacaga taaactcctt tactaccaat taaggtatgc
     2221 tctctttcgg gaaatttttc taaaatcgat tcgcaaccgg ctaatcttag cgcgtcaaat
     2281 actttacttg ctggcgcttg aggatttgct atagaaacgt tatcgtaaat actctgattg
     2341 aatagtttcg tatcttgaaa gacaaaagca attgtgttaa taattgtgtc ttttgaatag
     2401 tgatttaaag gcttaccacc aattttaatc tgtcccttat ccacattgta gaaacctgaa
     2461 attagtttag ctatcgtact ctttccgctt cctgattttc ctactaatgc ataaattctt
     2521 ttttgttcta aaactaagtt gaaattttct aaaacacact cttctttgta tgaaaagctg
     2581 acattttcaa atgcaatgtc acaattttta atctgtgtct cgttcccata ttttagttgg
     2641 tcttgcttca tttctaaata aagggagtct agtgtttcga ctgcatatct tgcttcaaac
     2701 aagtgcatac ttacatacat tattctcatg aaagaaacaa aaataattcc acttaaaaaa
     2761 aacaacatca acaagtcaac agctaatatc ttgggagtgg taagagaagt tataaaaaag
     2821 gttataggaa ctatcaaaat gcaaactatg ccaaaaaata gccattgaaa aagaacaaaa
     2881 ggtgttttcc cactcaaaga atacttatat gcaaacttag cataatcgtt aattgcttta
     2941 ttcaaggctt taaatgatgt tactttagca ccaaatatct ttataacttg aattccgcgg
     3001 atatattcta ccgtttcaga gcttaacaca tctaacgatt cttgatattt ttgaataaaa
     3061 tttgtattct ctgtcatttt ctttaacaag aatacaccaa ttactaacat ggcgattaga
     3121 accaatccaa ttttaaagtt aatgaaaaag cctaaggcaa ctgataaaac aggtgttact
     3181 atggcttgag cactatctgg aatcaagtga gcaattgcca tatgtgtttt agccgcgttg
     3241 tcatcaattg tctttcgaat atatcccgaa gaatttaaat caaaaaaacg aaaacttgct
     3301 tctgttaaac cgctaattcc cttttttctc aaattagtct ctaagcgaaa cccaaggata
     3361 tgagaaaata agccagaaaa aatataaaat aaagctccta aagttaacca agcaacagtt
     3421 tgaaccgcat aactcttagc taaatattca tctcctacaa taattagaga ctgaagaaat
     3481 ttgtaaatac tgtaatatcc atagactgtt aatacgacag aaaaatcaga aaataaagtg
     3541 gctaaccatc cgtagtattt cattttaggg acataactaa ataatttttt gtatactttc
     3601 attttttcct cccccgcatt caatttcttc tcttattcgt ttacaactgt taatgctttc
     3661 cgtaatagcg taaattagag atgtacaaac agataaaagg taaactacgt tctatttttt
     3721 gtattttttt aagacttttc ttccaatcaa tacaccaatc atagataaac ttgctacaac
     3781 taacactgaa atgattcccc atatacctac cgcatagtgg atcattgcat ccattgttgc
     3841 agcagattgt cctttatcta tccaatcttg acgaaaacga tctgtaaaga accatatagg
     3901 aatccactgt ccggcagcac tgcctaattg cattagagcc caagacgcaa cttgaggaat
     3961 tgctcgatca tatccaaata cagatagaat aatatcagct aagatcgcca tactaatcga
     4021 actgataaac caaggtaagt acgccccgcc catt
//
LOCUS       DACCXW010000179         3969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCXW010000179 DACCXW010000000
VERSION     DACCXW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.187"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            15..1955
                     /locus_tag="IVY71_002720"
     CDS             15..1955
                     /locus_tag="IVY71_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7986737.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2042..3964
                     /locus_tag="IVY71_002721"
     CDS             2042..3964
                     /locus_tag="IVY71_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7986738.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
BASE COUNT         1334 a          685 c          838 g         1112 t
ORIGIN      
        1 aaggcggtag gaaaatgaaa aaaattattg caatcacatc ctgtccagtt ggtattgccc
       61 atacatacat ggcagcagaa aacttagaga aagcggggaa agcagttggc gccgaggtaa
      121 aagtcgaaac acatggttcc atcggtattg aaaatgaatt gactgcaaga gatatcgaag
      181 aagcagctgg tgtaatcatc gcagcagata ctaaaataga taaatcacga ttcggcggta
      241 aacctttgat aaccgtaggt gtgcaagaag gaatccacca tgcagatgag ttggtgagag
      301 atattttagc aggaaaagcg ccagtctata aaagtacgga ggcaattatt tcttccgaaa
      361 ataaaagtga atcagaaaat ttaggtaaaa aaatctataa gagtttgatg aatggcgttt
      421 cgtacatggt acctttcgta gttacaggag ggttattgat cgcgatttct ttaacattag
      481 gaggaacagc tacacctgaa gggatcaaga taccagaagg aacgatttgg gcaacgatga
      541 atagtatcgg aagtatcgct atggggttga tggttccaat tctatctgca tttattgcgc
      601 aaagtattgc agatcgtcca ggacttgttc ctggatttgt tggtgggatg ttagcggcaa
      661 acggagcatt atatggtagt gatgcgaatg cgggattctt aggcgggatc atcactggat
      721 ttttagcagg attcattgct ttaggaatca agaaagtgcg agtaccgaaa gctttacaat
      781 cgatcatgcc aattattgtt atcccaattt taggatcatt ggcagttggt tttgtgttta
      841 tctatgttat tggagaacca gtagctcttc tatttagttc attgactcac tttttagcag
      901 ggatgcaagg aggaagtgaa attgttctag ctatgatctt aggagcgatg attgcttttg
      961 atatgggggg gccgttcaat aaagtagcct ttttgttcgg atctggcttg attgcagaag
     1021 gcaattattc cattatgggg ccaattgctg tagctatctg tatccctcca ttagcattgg
     1081 ggctggcatc attgatttta aaaaccaaat ttacgaaaac agaacgagaa gctggtaagg
     1141 cgtccttagc aatgggactg tttgggatta cagaaggcgc tattccgttt gcttcaacag
     1201 atccactacg ggtgattcca agcattgtaa tcggggcaat gacaggaagt gtgatcgcga
     1261 tgctttcagg agtgacagat catgtagctc atggcggtcc gatcgtagcg gttttaggag
     1321 cagtggatca cgtagccatg ttttttgtag cagtgattgc tggtattgct gtaaccgtgc
     1381 taatgttaag aatccttaaa ccgacattac aacaaactgc ggttgctgca gcaaatggtg
     1441 tgacagtagc agatctatca gagactgaaa caagtgatgt ttatacgaaa actgaaactt
     1501 ttgattctat cacggatatt atgtcagatg aagcaatcgt tttaggtgaa acagcaacaa
     1561 caaaagacgg agtaatcgaa gatttagtcc aacgtttaga aaaaacagcg gcgatcacag
     1621 ataaaaccag atttaaagaa gtcatctatg atagagaaca tgaaagtaca acgggaatcg
     1681 gtatggggat tgctattcca catggaaaat cagcttatgt tcttaaacca acagtggcat
     1741 ttgccagaag tcaatctggg gtagattggc attctttaga tggaaaacct gcacatatga
     1801 tctttatgat tgctgtacct gaaaacagcc aaggtgatat gcacttgaaa atactgcaac
     1861 gtctatcacg taaattgatg gatgatgact tccgtcaagc actgatggaa gcgccagata
     1921 aaacaacagt tcaccaatta ttaagcgaaa tgtaacataa gatgaaaaat cgaccgtgat
     1981 gagacataat agatgtgtta tcatgatcga ttttttattt agaagggagt ttctgcacat
     2041 tatgaatcag cgtcagcaat ctttgatcta tcaattaagt gagaacaata attatataac
     2101 aacaaaaaag ctagcagagc atttccatgt gtcagaaaga acgattcata gcgatttgaa
     2161 taccattgaa acttggttga gagacaaaca tctgccttta atgtttgaaa gaaaaaaagg
     2221 tacaggtatc ttgttgaatg gtctgcctca cgaaagggaa caactgaaac gagcgcttaa
     2281 tgaagagact caaacagaaa tggataaaaa acagcttcgt cagttattga ttttccatct
     2341 gctagtagca aaagacggat ttagtcttga agaactttca gaaaaactat atgttggaaa
     2401 acgtacgatt cgtaaagaat tgacagagtt gcaaagcttt tttgagtatc accatttaca
     2461 gctagtatcc aaaacaaaac taggaacttt tcttaaaggc gatgaacaag aaaaaagaca
     2521 gttgcttgtt aagacattac gaaatatgca gaaagaagat ccacaaagtc cgtcgttgaa
     2581 agaattcttc gctaaagata cattaaaagt tatccaacat acattacaag atgtttttta
     2641 tgaaaaccat ttagaacagc cgaatggact ggctagtgtg gagattcaca tctactttat
     2701 gctcgagcga atgaaacagt accagaaagt gaaattaagt aaagacgaaa acgaagtcgt
     2761 ggaacacacc caagcacaaa agttaagtag tcaaatatta gcaaaactag ccaccatcta
     2821 tccaattgaa ttttctcctg atgaaatcaa ttatctggct ttgcgtatcg ccaatctttt
     2881 tacaaattca caagcagcca cacgttttca aaaggaatct agcgcacttg ctgatcattt
     2941 gatcgaacaa gtggaacagt ttctaggcta ttcattaaaa gaagatcagc tactaaaaca
     3001 aaatttaagg tctcatttat cttcaactta ttttcgactc cattatggtt tgcagatttc
     3061 taatccattg acgaaaaatg tgtttagtac gtacacacaa cttttcttag ttttacagtt
     3121 aattttagaa gattattttg aaaaggaaaa tttttatgtt ccacaagagg agacagctta
     3181 tttaacgatc catttccaag cagcgatcga gcgacacaaa catcagaaaa gtagaagata
     3241 tcaaacggtt ttagttagtg aatattcaaa agctatggct acttttctcg aagcacggct
     3301 gaacagagaa cttccagaat taacgattat ggatctagtt gaatataagc cagaattaaa
     3361 gagacaagat tttccctctg ttgattttat cttggctacg ttaccattta aacatataag
     3421 cattccagta atcgagatct caccaatgat cacagaaact gatctagcct atttgacaaa
     3481 atatatgctg gagcatgtgc ctatcaagaa gaaaaaaaca tttgatctag caagctttac
     3541 tcatccattt ctgatctttc ctcaacttga acggacagac ccagccgata ttttaaactt
     3601 tatgggaaac gtcttagtgg aacatcatta tgtggagccg gaattcatcg attctgtctt
     3661 ggaaagagac agtcactcca gtactagagt agcaccattt gtaacaatcc ctcacggtaa
     3721 tccgctgtat gtgaagcatt cgatgatttc tattgctaca atgaaagaac cagtcctatg
     3781 gcacggggaa cagatacgta tcgtactgct attagcaata aagaaaaatg atttgaaaca
     3841 tgcagagttc aaaaaaatat tttctgttat tcactatcta gaaaagcagc cagaacaaat
     3901 ggctcagttg atgcaatcca atcatccgct agaaatctta acgttgttat ctagttatga
     3961 ataagtagc
//
LOCUS       DACCXW010000180         3945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCXW010000180 DACCXW010000000
VERSION     DACCXW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.188"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(33..491)
                     /locus_tag="IVY71_002722"
     CDS             complement(33..491)
                     /locus_tag="IVY71_002722"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7986739.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(662..1807)
                     /locus_tag="IVY71_002723"
     CDS             complement(662..1807)
                     /locus_tag="IVY71_002723"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP7986740.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1820..2737)
                     /locus_tag="IVY71_002724"
     CDS             complement(1820..2737)
                     /locus_tag="IVY71_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7986741.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3165..3728
                     /gene="efp"
                     /locus_tag="IVY71_002725"
     CDS             3165..3728
                     /gene="efp"
                     /locus_tag="IVY71_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7986742.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1226 a          743 c          759 g         1217 t
ORIGIN      
        1 tattgcttct catctttacg ttcttttaga aactatgctt ctacttcaga ccattcatcg
       61 cgttttgcta aaaattcacg agccaaagct tgtgcacctt ctagtgtatg gctggcagcc
      121 cagccacatt gtgtttcatt gcttgcaggt acttcagtag cttcaagaac atctttcatt
      181 gttgcttcaa gaacatctaa aatgtcttcg tagctttcat gattcaatac agtcaagtaa
      241 aagcctgttt ggcatcccat tggagaccag tctacgatgt aatctgcgtg attgcggatt
      301 agttcggcag tcaagtgctc aagtgaatgt agacttggca tgtccatgtg ttctttattt
      361 ggttgtttaa agcggacgtc gtatttaatg atgacatcgc catgcgcacc cgtttttcga
      421 tcagctacac gtacataagg tgctttgact tttgtatggt caagattaaa actttcgaca
      481 ttcattctca tttgtaagac tcctatctgt ttgttcattc gttttgatgc ttttcactaa
      541 agaattattt agtgaaattt acaaaaacgt ctttctactt gattctaaca cttatcgttt
      601 ccaaggtgta gcaatctgaa atatttttta gatttttctg ctaagtcgtt tattgggact
      661 tctagttctt gattctgtca aatgcctgag ctaaatcgtt tatgagatcc tgaccatact
      721 caattcctac agaaagacgg atcaagccgt cctttatgcc agcctcttct cgtttttctt
      781 ttggaataga cgcatgagtc atgacagaag gaatttccac taaactttct acgccaccta
      841 aactttctgc taaagtaaat agttggagtg attcaacaaa tggaatagca gattcttcat
      901 tttttaaagt aaaagaaatc attccgctaa agccgctcat ctgctgctta gcaactccat
      961 ggagttcatg atcgggtaat cctggatagt agaccttttc tactgctgga tgagaaaata
     1021 gaaaatcagc taccacaaaa gcattttttt gatgttcctc catacgaaca cttagtgtct
     1081 tgattccgcg ctgaagtaac cagctatctt ggggaccaag tacggctccg atagcatttt
     1141 ggtaaaatcc gatctgatcg gctagatctt tatggttaga agtcactaaa ccagctacta
     1201 catcgctgtg tccgcctaga tatttggtac cgctatggac aacgatatca gaaccgagat
     1261 ctaaaggtct ttgaaaataa ggagtagcaa atgtattgtc agcaataact atcaatccat
     1321 gttgatgagc cagagtggca cttttttcta aatcagtaat ctttagaagc ggattgctag
     1381 gtgtttcaag ataaagtgct tttgtattgg gtttgatgga ttgttctatt ttatcaagat
     1441 tactcgtatc tatgatcgtg tactcgagac catttttagt aagaactttg tcaaatagac
     1501 ggaatgttcc tccatagaca tcatctccta ataagatatg atcgccagct tgaaaaagag
     1561 aaaaaacggc atgaatccca gaaagaccag aagaaaaagc gaatcctcga acaccgcctt
     1621 ccaaatcagc aatcagttct tccaatgcaa aacgagtagg attaccagat cttgaatatt
     1681 catattgttt aggctggccg acgccatctt gccgataagt ggatgtttga taaataggta
     1741 cactgacagc tcctgttgtg ggatcttcac tgatgcctcc gtggatcaat tttgtttgaa
     1801 tgtgcatgtt tctttcctcc tataagtaaa tgttttttga taaatagcgg tctgctgcgt
     1861 ctggaaaaat cgtaacgaca cgatgaccag gtggtaaacg acgaatttct cttaatgctg
     1921 ctgcaaaagc cgcaccactc gaacttccaa ctaatagtcc ttgttctcgt gctaactgcc
     1981 tagtatagtt aaacccttct acatcagaga ttgtctcgat ctgattgatt gaaaggggag
     2041 aaagaaacgg cggaatgaat tctacaccga taccctcaat ttcgtgaggg gctggatcac
     2101 caccatttaa cacagagccc tctggttcta caccgattat tcgaatgtcg ggatattttt
     2161 ctttcaaaaa ggtagaagtt ccagcaaagg ttccaccact cccaattcct gctacaaaac
     2221 tatggatatt ttctttgatt tcatgaaaga tttctggccc taacgtatga tagtaagcag
     2281 caggattatc tttattttca aattgaagag gcagatagct gtttgatatc tcctctgcaa
     2341 gttttttact tttgctgatg gcaccgatga ttccttgctc actaggagaa tggatgatct
     2401 ttgctcccaa tgctttcatc aattgttgtt tttctaagct aaacttctct ggaacaacaa
     2461 aaaccgttct taatttgtat gtgagtgcgg ccagagcgat cccaattcct gtatttccgg
     2521 cagttggttc gatgatcgtc gttttttcat tgatttttcc ttgggcaaat ccttctttga
     2581 tcaaataact gcccaatcta tctttgatac ttcctccagg atttaaatat tctagttttg
     2641 cataaatagc agaaccttta ggcatagaat agtgctgatt gtcaaagcga taaagcggtg
     2701 tggaaccaat agcttctgta attgtcgaga taatcatgtg ggcctcctaa aaaaagataa
     2761 taaaaaaagg agctgactat taaatcagct ccttgaaaga catagagaaa aattgagtag
     2821 ataaaaagat aagttacctg gtactcattc tatgaagggc aattaataga catgacaaat
     2881 agtatgctgc ttttatgcac catacaacag caacgattca attgtttttg gttgctgact
     2941 gtcatgtgta tccctccaag taaaattttt attaacgata gcatgaaaaa aaataaaagt
     3001 caaaggcttg aaagaaaaaa atatttcgat agcatcgttt acccgtgttt atttttctga
     3061 caaataggca tgagacctta aaagatagag aatactctag ttttctggcc ggttgtctga
     3121 tacaataaag ttgattatga taaagaaaag aaggagcaaa aataatgatc tcagcaagtg
     3181 atttaagagc cggtatgact ttcgtacaag acggcaaact gattaaagtt ctggatgcaa
     3241 gccaccataa acctggtaaa ggaaatacag ttatgcgtat gaaactgaaa gatgtccgtt
     3301 caggtgcaac atacgacaca acgttcagac cagaagaaaa atttgaaaaa gcacatatcg
     3361 acacaaaacc tgtacaatat ttgtacacga tggatgatac agctttcttc atggatttgg
     3421 aaacatatga gcaatatgaa attcctgtag aaacagtaga agaagaaatg aaatatatcc
     3481 tagaaaacat ggaagtaaaa atccaatttt tcggtagcga agtcatcggc gttcaattac
     3541 caacaactgt cgtattacgt gttgtggaaa cacaaccttc catcaaaggt gcaacagtca
     3601 ctggttcagg taagccagcg acaatggaaa ctggcttagt agtcaatgtt cctgacttca
     3661 tcgaaacaga tgaattgttg gaaatcaata cagcagaagg ttcttatgta aaacgtgcgt
     3721 caaaataatt cgctaacgga ttgataagaa tttgaaaaca gagcttgacc tcttgaaagg
     3781 cagatcatcg gatctgcctt tcaagaggtt ttttgtggaa tcagcaaatt gaaaaataaa
     3841 aaagtatttg attttattta tattttgaaa gatgaatggc aaaagctaga gaaagaaaaa
     3901 actgcactta tttatgaaga aattgaaatg attcataaaa ttgaa
//
LOCUS       DACCXW010000181         3876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCXW010000181 DACCXW010000000
VERSION     DACCXW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.189"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(716..3865)
                     /locus_tag="IVY71_002726"
     CDS             complement(716..3865)
                     /locus_tag="IVY71_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7986743.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          635 g         1362 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttggacact tgaatctcgg ctgttttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cttccttcac gaatgggctg attaggaaac tcttttgtta
     2041 accagtcagg ttctttcgtc atttctttga tcgctcgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg
     2161 tataggcatt gtctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagt agggtccaaa taactttctt ttttgacagg ctccatttga tcaatcatac
     2281 gattgatctg ttccggtgaa tactctgcat atgtttctac ttcttttaat ttccgataga
     2341 ttttattctc taatgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaaa
     2401 ccgagccatc ttctaatgca ttcttgatgg tataggtatg caagacctct ccatactgat
     2461 catgggtcgt acgtgccaat tgccctttgg cttgtttccg attctcaggg aaaatcggtg
     2521 tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt
     2581 cagcacttaa cgctcgatga cactcatcta ctacaaacac gatatgttgc cccattaatt
     2641 tttggaagcg attggttcct tttttctctt cttgtttttt tgcatatttc aacgccgcat
     2701 ctagcttttg acgggttgta ataatcacaa catttgcatt ggcatctgcc aacagagttt
     2761 cacttaactc tttggcactt cctgttccca cgatcaagct attcgctttg gcgttcccgg
     2821 aagaaatccc agtattaaat tccgaagcaa atttcgtaaa ctctgtggtg gtttgattat
     2881 ctaaatcttt gcggtccact aacatgatcg tccgatcgat ccccgatttt cgtgctaaaa
     2941 gcttagtaga tacaaaacta gtcaacgttt ttcctgaacc agtcgcatgc cacacatatc
     3001 ctgattgatg tttatttgca gcaataaaca aggcttgaat cgcatgtact tgataaggat
     3061 gtaacaccat gaggatttta ttgtcttgat cttcactgac aatcgtataa tccgcaatca
     3121 agcgatgagc atccgggata tttagcactt gtttgcaaaa ctcatataag ttttctacct
     3181 ttttattatc tttcgttcgc caactaaata aaaacttagg atgcaaatcc tttggtaagg
     3241 catttgcaaa ataccgagtg gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa
     3301 agatattgtt ccgaaacata ccttcttctg catatttttt gatttggtta aacgcctgat
     3361 aaaaactatc ttttgcggtt acttgtttca gctcgatttg gacaatcggt aatccattga
     3421 tcagtaaggt gacgtcaaaa cgtcgatccc gtgcttctat gttacttcca cgttttgcaa
     3481 tttgatggac cacttcatag gtagagattc ctccaccaat gtcttgatta gaataaagaa
     3541 tgagcgagac agaacccagt tgcggatctt cccgttcaat cgtgatccga gccattccat
     3601 tttcaccttt taaccatttc gcagcatcaa agggggtttt cgttcgtagt aacaattctg
     3661 ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc attcagttct gcttgattat
     3721 ttgagatgat tttttggcgt aaattgaccc aaagatcttc ttctgatttt agatcgggac
     3781 gatacgtcca ctgattatgc ccttccccca atacacggat caactgttct tcgacttccg
     3841 cttcctcacg gtgactaatt ttcatttatc ttccct
//
LOCUS       DACCXW010000182         3849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCXW010000182 DACCXW010000000
VERSION     DACCXW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.190"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..720
                     /locus_tag="IVY71_002727"
     CDS             <1..720
                     /locus_tag="IVY71_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7986744.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
     gene            824..1048
                     /locus_tag="IVY71_002728"
     CDS             824..1048
                     /locus_tag="IVY71_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986745.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            1061..1561
                     /locus_tag="IVY71_002729"
     CDS             1061..1561
                     /locus_tag="IVY71_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7986746.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            1658..3505
                     /locus_tag="IVY71_002730"
     CDS             1658..3505
                     /locus_tag="IVY71_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP7986747.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            3508..>3849
                     /locus_tag="IVY71_002731"
     CDS             3508..>3849
                     /locus_tag="IVY71_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP7986748.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQ"
BASE COUNT         1377 a          522 c          807 g         1143 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatatat caatggtttt taaccaatgc tcaatcttta gtcttactag cgattgtggt
      901 cattggtttg tttttaggat ttaaacgcga gttttccaaa ctgattggct tcttggtgat
      961 tgctttgatt gcggtaggtc tggtctttaa tgcttctggt gtgaaggatg tcttactcaa
     1021 tttgtttaac cgaattgtcg gtgcctgagg gggaatcaca atggaacaga tacgtgttta
     1081 tattgcgaac cttggaaaat acaatgaagg tgagttggtg ggggcatggt ttaccccacc
     1141 cgtcgatttt gatgaagtga aagaacggat tggattaaat gacgaatatg aagagtacgc
     1201 aattcatgat tatgaactgc cttttgagat tgatgagtat acaccaattg aggaaattaa
     1261 tcggttgtgt ggtttagcgg aagagcttaa aggaacgcca attggtgagg tcgcttcaga
     1321 gattcaacat gccttcttca attctttcga ggaaatggta gagcatgtgg atgatatcat
     1381 ctattatccc gattgtaatg acatgagtga tgtagcgtat tacttgattg tagaagctgg
     1441 tgatttaggc gaagtaccag aacacttgaa gacgtatatt gattttgatg cgtgtggtcg
     1501 agacttggag attggcggga attatttggt tacgagtcga ggggtttttg aactaatgta
     1561 aatcatttgt tagaattgac cagaaatttc tatcgtactt ttacttttct atttgatata
     1621 ctgaaataga aaatatttgt tgataggggt gttactaatg gttggcaata agtggtacaa
     1681 atgtgatctt catattcatt caactgcgag tcagtgcttt caggataaaa aggttactgc
     1741 taaggaatgg gtagaagaat gtttaaacaa aggactcgat tgtgttgcat taactgatca
     1801 caatacaggg gaaaatattg atgaatataa gaaagtggct atcgaaaatg gcttagattt
     1861 ttttcctgga gtggaattaa cgtgtggaga aaatggaact catttactag tcctatttga
     1921 taaaagtgca tgtacaaatg atatctctga tttcttaacc aagatgggta taaaaagaga
     1981 ggattgggcc agcgctgcag cttgctcaat gaaatcagtt ttagaagtta ttgattttgc
     2041 tgagcacgag ggcttgctcg ttattcctgc acatatagat gagtttaatg gaatttcttc
     2101 agcaaaattt ggaaatctag gaaagatatt tgaatcagaa aatatatatg cagtacagac
     2161 ggttcaaaaa gaattttttg aaaatcgctc acaaatgatt ccctcatcta agcgtgacac
     2221 tatctatgat agtgtgaatg ccagatatga tggtagagtt ggaaaagata ctttggaatc
     2281 ttggtataaa tcagttgttg aggcagaaaa aaataatatg acatttctta gcttttctga
     2341 taacccacac tcgaaaggaa attcaaaaca tggactttgg ggaataggca caagatattc
     2401 ctatataaaa atgaaggacg aacctgactt gggatcactt cgcgatgctt taatgctagg
     2461 cgaaactcga gttcattctg atttttctaa tttctccatt aatgaaaatg aagttttatt
     2521 ggaaaaatta agcttttctg ggaccactct tagcacaaaa gaagttgtag tcgaattcag
     2581 cgataattta acttcaatta ttggaggaag aggaacaggc aaatcttgta ttacaagatt
     2641 cttggtctat gtccttggaa aggaagcaga acttgatcaa ttttctgaaa tacaaagtga
     2701 ttatcagaac tttgcacaaa ttgaacataa tggctctgga atatttttga aggatacaat
     2761 tgtgaaactg aatattttct ataaggggac aaaatatcaa ataataagaa cacaggatag
     2821 acattctatt tatgaattta cacaagagaa tggtctggtt gaaaaagaaa cagaacggtt
     2881 gagaatgatt tccgataagg tgagtatata ctctcaaaaa caaatttatg aaatttcaaa
     2941 aaatcaaact agtatactag aattactaga tggatataac tctgatttga tttcagagta
     3001 taaaaatgag attgaaacat gtgtaaacga aataagaaaa ttaaattggg atattgtttc
     3061 tgtgaaaaaa gaaatacaag ataaggcgaa ggtagagctt gaaattgaag atttaaaaaa
     3121 acaagtagaa aagctatctc ataagagtta taaagatgtt tatgatgagt attcaaaaga
     3181 gacagatatt tatagggaac tgaaacgtga tgtggaggca cttaaggaaa ttcccaaaaa
     3241 tctaactgat agtgttgaca aaattgactt tggcaatggt ataaaagtta cagacgaaat
     3301 tgatgaacac cgcggagaat tgattaagaa tttagtgacc aatcgggcta agattcaaac
     3361 aataattaat gaaatgtcag atgaaattga tagttataga gcagagctga ataaatcaga
     3421 ttggaaagtt aactataatg aggtttcaaa gaggtatcaa agaggtatca aaaacttagt
     3481 gaagagttat ctcaagatga attaaaaatg ttacaaaacc ttgattcttt atatcaatct
     3541 ttatcaaaaa ctgaagagcg attggaagaa attattcaac aagaaataaa gcttaaagat
     3601 taccaagttg aggttgactc agcaatgtca agaatgaata aatcatatca aaatctctat
     3661 tctgagagaa gtagttcctc gcgcgaaatt ttttctgata ttacaggaat agaggctact
     3721 attcaaccaa atagggattt caataattat gttaaagagc tcagagcttg tattaataag
     3781 gaggacactt ttgacagtga gtttggatct cttattgata agctgacaaa aaattccatt
     3841 actaatcaa
//
LOCUS       DACCXW010000183         3721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCXW010000183 DACCXW010000000
VERSION     DACCXW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.191"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..356)
                     /locus_tag="IVY71_002732"
     CDS             complement(<1..356)
                     /locus_tag="IVY71_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986749.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(616..1164)
                     /locus_tag="IVY71_002733"
     CDS             complement(616..1164)
                     /locus_tag="IVY71_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986750.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1165..2022)
                     /locus_tag="IVY71_002734"
     CDS             complement(1165..2022)
                     /locus_tag="IVY71_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7986751.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2308..2594)
                     /locus_tag="IVY71_002735"
                     /pseudo
     CDS             complement(2308..2594)
                     /locus_tag="IVY71_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2584..2913)
                     /locus_tag="IVY71_002736"
     CDS             complement(2584..2913)
                     /locus_tag="IVY71_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7986752.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2990..3262)
                     /locus_tag="IVY71_002737"
     CDS             complement(2990..3262)
                     /locus_tag="IVY71_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7986753.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3262..3525)
                     /locus_tag="IVY71_002738"
     CDS             complement(3262..3525)
                     /locus_tag="IVY71_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7986754.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1092 a          754 c          473 g         1402 t
ORIGIN      
        1 ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac taataatact
       61 tgatctcgct tattataatc gctttcattt atcctaagag tcaaatcttt ttccaccata
      121 acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct ttgtgtagcc
      181 tttaaactga caattagctc attgggtaca tactctattt gagagtcatc taattggctc
      241 ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc catgaaccca
      301 aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt tttcatctac
      361 atctcctcct aatagtttta ttttagagtt agctctatta cttatttact aactcaacaa
      421 cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca ccctaaaatc
      481 ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa aaataaatcc
      541 cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg tagttactta
      601 gttcttcttt gattattatc taactttatt attcattccc caccctttgc gcagtgagtc
      661 ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat catctaattc
      721 tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt cattaaacaa
      781 atcttgctta tcttcttgcg taagattctg tttaatatac tcatctaaag cttttctaag
      841 caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg aagtcgcatt
      901 taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa actttctgat
      961 tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg aggaagtttt
     1021 tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa ttgcttgctg
     1081 ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt tcccaaaaat
     1141 atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct cgtgttcttc
     1201 caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt gttttaagag
     1261 acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt
     1321 tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt gtttatacat
     1381 ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag caacatataa
     1441 atttgtatca aattctttca caaaaaagtc catatactca agatattgca tcgcatttct
     1501 catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt acatcgcgga
     1561 tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa actcatcaaa
     1621 taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat tatattggtc
     1681 tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat agaggttatt
     1741 gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt cagtcacagt
     1801 attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga agttcatatg
     1861 cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta tcaaggttct
     1921 ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac ttttacctac
     1981 gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac ctcttttctt
     2041 ttagcaatat tcatttgata ctattataac atatttattt ttagttacta ccaaaaaaaa
     2101 gatatatatg tactacctta taattaataa gtaaaaggta gtacataaat aaggaaaagc
     2161 tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt tatcgagaag
     2221 cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa aaaagaagcc
     2281 gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt
     2341 ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac
     2401 tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt
     2461 tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt
     2521 ttctttcaat ggcttctctt ttagcttttt aaatacttaa tgacatgttt tgaaggaacg
     2581 atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag
     2641 tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc
     2701 tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct
     2761 aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa
     2821 ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat
     2881 tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt
     2941 aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact
     3001 cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa
     3061 caacccggtg ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact
     3121 tctcaaatga ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa
     3181 attgaggttt caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa
     3241 tcatcacaga atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa
     3301 tccactatca tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa
     3361 tagcgtttct ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact
     3421 gcgctcacta atgatttcaa tttccgtatg attctcattt acatctttca acaacttata
     3481 aaaatttttc cgtgctgagc taggatttaa gactgttttt gccatatcta ctccccctct
     3541 aatttatatc tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa
     3601 attggagtac caaaacagca ttgtaaagtg ttatccgttt cctttatacc cgtggtgcta
     3661 gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat
     3721 a
//
LOCUS       DACCXW010000184         3706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCXW010000184 DACCXW010000000
VERSION     DACCXW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.192"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(132..323)
                     /locus_tag="IVY71_002739"
     CDS             complement(132..323)
                     /locus_tag="IVY71_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986755.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(464..784)
                     /locus_tag="IVY71_002740"
     CDS             complement(464..784)
                     /locus_tag="IVY71_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986756.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(807..1241)
                     /locus_tag="IVY71_002741"
     CDS             complement(807..1241)
                     /locus_tag="IVY71_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7986757.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1576..2214
                     /locus_tag="IVY71_002742"
     CDS             1576..2214
                     /locus_tag="IVY71_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7986758.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(2538..2621)
                     /locus_tag="IVY71_002743"
     tRNA            complement(2538..2621)
                     /locus_tag="IVY71_002743"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2585..2587),aa:Leu,seq:cag)
     gene            3149..3649
                     /locus_tag="IVY71_002744"
     CDS             3149..3649
                     /locus_tag="IVY71_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7986759.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT         1089 a          735 c          594 g         1288 t
ORIGIN      
        1 gacgctttca gtaatttttc ttattatttg gatctatttt ttatataaga atcagaattg
       61 aatccctcgc atgtttaaaa agaaaacgat taaagatgca gcaaatttct gccgatcttt
      121 aatcgttttt tctattctgt tataccaaaa aaatgtttga atcggtgaag gatcgttcct
      181 tgttttaatt ctataaattc ttgtgcgatc gggtactttt cttccaaatt ttctttgttc
      241 ttttcgatca ggcttttcgc ttcttcatat tttttatctg cacatagtgc ttttacttcc
      301 tgtaatactt ttcctgcttc catttgcctc accctcttgc cattttattg ctttaagttt
      361 tatttaagtt atacgcatta ttctagtttc atcccaaata accttttttc atttttactc
      421 ctcgaactaa tcagttttct aaaggctgat tggttcgtct tttttatttt tcctccattt
      481 cattctgctc ttctgttaat ttatctaatg ctttgattga tgctgcggct cttgcataat
      541 catagctgta taattccata aatttatcag gcagaacaaa ccacgctgga tgataaccgt
      601 tagctgtgac agggaatcct ttatcttgtt ggttatctcc atcccatctc agagcaacac
      661 ctttttttcc tttccacttt actaaagcga tcgagtatcc cttctttgtt tgtgtgccgt
      721 cttcaataat tgctaaaatt tctatttttt cttttggcgc acggacttct tgaggacgaa
      781 ccattttcta ttcctccctt atctcattac ttcacgacca acacactgca aggggcatgt
      841 gatacgacat aagaagcggt cgatccaact aatgcttgtt gtatagcacc ttttcctgtc
      901 gctcccataa cgatcaaatc aatattttct tgtccaggga tgacattggc aatcaaattt
      961 cttggatctc cagtttcaac tattgcgtgg atctcagcaa tgccgtactc tttggcatca
     1021 ttgatttttt tcagcatggc tacttctgtt ctttgttttt catctgcata gatttttgaa
     1081 aaggcatagg cactatgggt cagctcaact ttgttgatga ttgacacgat aaataagtgg
     1141 gcttcattgt cctttgctaa gtcaagggct tcagcgaacg ctttgatcga ttgtcttgat
     1201 ccgtcaactg ctaccatgat gttctggtat cttttactca ttggaaccgc ctcctttttc
     1261 ttttattgta acatctcaag caaaaataca aaaaatatac gcaatatgta aataaaatga
     1321 tgaacaaaag caatttcttt caagttattt atacatttct tttctcatca gaacacctgt
     1381 tctaatccca ttatttctgc attatgtgaa attcctcatc atttttcaca tcgagcgaac
     1441 atcattctgt tacaaaaaac gtggatttgt cataaattgt catgcttctt ttacctatga
     1501 aatattttca cgtgttaaag tattcctcgt tgaacacaac aaataaaact ttttaacgaa
     1561 taaaggagat ttttcatgac atcacttaaa actttacttt ttggaacaac tttggctgcc
     1621 ggcgctgcat tcttcatggg aacgactgct catgcggacg aagcttatac cgttcaatca
     1681 ggtgatactc tatcaacgat ttctcaaaaa tacgttggtg ataactcctt gatcaatgcc
     1741 attgcagaat caaattcaat ttctgatatt aacctgatct actcaggaca acaattaaca
     1801 atcccaacag aaggctctgc acaagcagct gctgaacctc aagcagccgt tcaagaagca
     1861 ccagtccaag ctgaacctgt tcaagctgaa caacctgttg tacaagaaac ggttcaaacg
     1921 gaaacacaag cagcgcctgt tgctgaaaca caacctgctc ctgctgtaac agaaactgct
     1981 gctactccag caagtacaag ctctgcaaaa gaatggatcg cacaaaaaga atcaagcggt
     2041 tcttacacag caacgaacgg acgttacatc ggccgttacc aattagattc ttcttactta
     2101 aatggtgact actctgctgc aaaccaagaa agagtagcag aacaatatgt tacttcacgt
     2161 tatggttctt gggaagcggc gaaagcattc tgggaagcaa acggttggta ctaagattca
     2221 ttatttgaac gtttaaacac attcaaaata tagtaagagg tcatgcgatt tattctttcg
     2281 catgacctct ttttgagttt aagtttagtc tttattcctt aactatcgtt tgccagtatt
     2341 tcttgtatca gaatctataa atctactatt tgaatcaatc tattaggttt taaactgtcc
     2401 caccttcaca caaatttttt ttgatttatt tgaatctggt tccgttgttt aaaatttttc
     2461 ctatattctt ttagataata tgttattttc tttctcatcg ctccaggtgt accttctctt
     2521 tataaaaaaa caaattatgc ggctaagaag actcgaactt ccacgaggtc accctcacta
     2581 gcgcctgaag ctagcgcgtc tgccaattcc gccatagccg catcacatct tttagtttag
     2641 gattacatgt gttttttgtc aagaacatat ttttgttctt tttcatttct gtacgccctc
     2701 ccttatgcca tctacacaaa taattcatcc gagcaactcc atcatctatt gagggttggt
     2761 gaaaaaatct gttttatcca tatgatcgat ttcaatgtta aacgaacgac tctgggatta
     2821 gtaactattt tatttttttc ttcatttctt ttttcctctt tttctcgatt cagctatctt
     2881 ctttcatttg gatattgatt atttcttctg ttgattacta tccttctgga attctagacg
     2941 ctgaagtaat tcacgttttc tttcttctag agatcataag taacgaatga acgaacttca
     3001 aataattttt ttattgtaat tcatatttgt attgttcgat gattcttaat acgatatgac
     3061 acaataaata aaaataggca tggctattta caacaatagt gctatagtag aaaacaaatg
     3121 aataaggtta cttaaagagg tgaatacgat gaaactgatc aaactatggg aagcagattt
     3181 aaagaaagct tatcagctac aaactagctt tggaccaaat gagagtggat ttatcaacaa
     3241 tgcttatggc tatacatttg ctgaattttt agattatgta aaaaaatgcg aaaaaacgtc
     3301 tttaggaatc gatcttcctg agagttacgt tcccgcaacc gtctttattt tagaaagtga
     3361 tgaacatgag tatgtaggaa tttttaattt gcgccataaa ctgaacgaaa cattaagaaa
     3421 cggcgctggc catattggtt acggcatctc ccctaattat cgtaaaaaag gatatgccac
     3481 acaaggatta gcattagtac tacaagaggc aaaaaaaatc ggcattgatg aggcactttt
     3541 gtctgtaaat aaaaaaaatc ttggcagtct taaagcacaa caaaaaaatg gggctgcgat
     3601 ttatcgtgaa gatgagaaag aatactatac gaaaatcaaa ctgatgtaaa aaagaaggat
     3661 tccaaaagga aagggatttg aatgtccttt atcaatagaa cacatt
//
LOCUS       DACCXW010000185         3690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCXW010000185 DACCXW010000000
VERSION     DACCXW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.193"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(74..736)
                     /locus_tag="IVY71_002745"
     CDS             complement(74..736)
                     /locus_tag="IVY71_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7986760.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            920..2566
                     /locus_tag="IVY71_002746"
     CDS             920..2566
                     /locus_tag="IVY71_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7986761.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2557..3639
                     /gene="uxuA"
                     /locus_tag="IVY71_002747"
     CDS             2557..3639
                     /gene="uxuA"
                     /locus_tag="IVY71_002747"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7986762.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         1196 a          651 c          745 g         1098 t
ORIGIN      
        1 tattttgtta tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg
       61 tcgcaagaaa atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt
      121 ctttcagcat catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat
      181 caactaaagc cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct
      241 gaatggtcac tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt
      301 tccaaacttc ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac
      361 ggaaaaaagc gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt
      421 ctagcactat tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt
      481 ccaactgttc tcggacaaag cgagcatttt ctgcagaaac aagattaatt tttgaaacat
      541 acgtaccact ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt
      601 cgcgaatggg agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc
      661 ttccaggttc taagtcagca tagataattt gtttacgaat cgcttgataa gcttgagatt
      721 gcaagctgct tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa
      781 gaggtctgaa aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa
      841 ttttggttga catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg
      901 ttagtttagg aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt
      961 atctcaaaaa gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca
     1021 aaaaaatcct gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga
     1081 aatcgctcaa cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac
     1141 attcgatgaa gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt
     1201 ggtcatgcca agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct
     1261 gtattgtcat ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc
     1321 tagtttacaa ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa
     1381 taataaatat ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca
     1441 tacaatgagt attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat
     1501 tgcacttgtc tctacagata atttttcaca aaacggtagt caattacgta cttctttgct
     1561 aacgatcgca aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt
     1621 atcgaatgaa gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc
     1681 ttccttgtca gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca
     1741 aaccgcaaaa ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat
     1801 catggaagat cattttccca atggacgtcc gaaattcgaa aaagctggag tattcttgac
     1861 tgatcgtgca acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt
     1921 gcatacgtct ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat
     1981 gaaagatcca gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt
     2041 tgtggagtct ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg
     2101 cttttctaat ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa
     2161 ggtggcgatt cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc
     2221 gcagacactt gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat
     2281 tgatgatcaa ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca
     2341 taagctttcc gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc
     2401 tcctattttg tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag
     2461 aaaaatagaa gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt
     2521 acaggattac ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac
     2581 ggatcacagg aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg
     2641 attgtaggca cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa
     2701 gaactaaaag aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat
     2761 gtgcatgatg ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag
     2821 cagacaatcc gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct
     2881 gtatttgact gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta
     2941 gcttttatat ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat
     3001 tccagcaatg aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc
     3061 ttgcttgaag cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt
     3121 ctccaatcaa tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat
     3181 gatccgccat actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat
     3241 tggttgtgcc gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt
     3301 gcagaagatc cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga
     3361 atcccatttg cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca
     3421 ccgcataaat ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac
     3481 aacggattta acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga
     3541 agaccaggat acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg
     3601 gaagcaatcg aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat
     3661 tatgtaaata agaaggatgt tcaaattctt
//
LOCUS       DACCXW010000186         3651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCXW010000186 DACCXW010000000
VERSION     DACCXW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.194"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(270..947)
                     /gene="nth"
                     /locus_tag="IVY71_002748"
     CDS             complement(270..947)
                     /gene="nth"
                     /locus_tag="IVY71_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7986763.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(954..1652)
                     /locus_tag="IVY71_002749"
     CDS             complement(954..1652)
                     /locus_tag="IVY71_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7986764.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1920..3104
                     /locus_tag="IVY71_002750"
     CDS             1920..3104
                     /locus_tag="IVY71_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP7986765.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3110..3553)
                     /locus_tag="IVY71_002751"
     CDS             complement(3110..3553)
                     /locus_tag="IVY71_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7986766.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          944 a          729 c          611 g         1367 t
ORIGIN      
        1 cctttagata gatacgtcac ttatttatat aatcctttgg aatactggac ttattttata
       61 ggtatattgg aaggcattat tttctacatc gggtattttc ccaataaaaa taacaacttt
      121 tatttttatc gcaataaatt gtaataagtt accatcctag ctactgagta tgtacattta
      181 ctttttcatg caaaaaagat aaacgaaaca gcttttctcc ataataacaa gataattcat
      241 acatttattt gcaattaaag aaaccacgtc tacaggcgtg gtttcttttt tagcgccttt
      301 tcttttagac gcatccgatt tttcccatct tgacatattg atagcaaagg acatacctcg
      361 cacttgggat ttcttgccgt gcaatgatat ctaccaaaaa aaatcagtgt atgatgtgtc
      421 tttacccata attcttgtgg tactttccgc atcagtgttt cttctacttc catgaccgta
      481 gcatctagtt tgcagatcct caggcgtttt gagacacgtt ccacgtgggt atctactgcg
      541 attgctggta tcccgaatgc atcccccaat acgacatttg ctgtctttct gcctacacca
      601 ggaagagaca tcaattcttc tcttgacgtt ggcacttgcc catcgaaacg ttcaatcagc
      661 tgctgtgcac acgctttgat gttttttgct ttatttcgat acaaaccaat cgtttttatt
      721 ttaagaatga tttcatcgat tgaagcttct gctaacgcat cgggtgtcgg aaaagaggca
      781 aacaaatctg gcgttgcttt attcactgat acatctgtcg cctgggcact taagatcact
      841 gcaatcaata actcaaatgg attattgtgc ttgagttctc catgtgcttc gggaaacatt
      901 ccatacatcg tttctagagc ttccattgtt ttttgtttgc ttaacatatt ccgctactcc
      961 tccaaccaat ttttcagtga gactttcggc aattcttctt gttgagctga ctgttctact
     1021 tctctttgaa gaagctgctg ttttcttcgt ttttggtctt cttccacttg ttgttttgta
     1081 cgtagatttt ttctttccca cgatagcaaa atgcggtcaa cgtaattaaa actataagcc
     1141 tgatttaata cggcttcccg cagtgctagc ttcaaaattt ccggctgata atggtcttct
     1201 tctagccatt gtccgatccg ctggaactcg atcgaagaaa gcgggcgtcc aaattcttgt
     1261 tcaaataatt gatacactga ctggatttct ttttcttgtg ttttttcttc gtattttttt
     1321 tcttgtgttt ttaaatactc acctaataga tcatagatgg ggtaaagatt atatcgatct
     1381 gcttttttac cgttgacaat cgtcgtttct atcttgataa atccatttgt tacgagacga
     1441 tccagtattt gaaaaatctg atcttgtttg attcccatat ccatagcaat catctgcaaa
     1501 tcagggaaag tatctccttc aagctgtgcc atatgcaact gtagaacaaa taaaaattct
     1561 tcagattgta gaccgatcct atgataattt ttaagtaaaa gattagatat gactgtttgt
     1621 ccagcgctta agtaattttc taaattcaac atgatttctc ctccgctcaa tcacagtata
     1681 cgtaaaaaaa acatgggaag caagaacagg tttcttttct atttacttca ctgatcggtc
     1741 agatttacaa tgtcgtctac ttgatttttg tttgttcatg tttttgatgc gtatcttcac
     1801 ttttcggaat aaataaatta agtagatttc gctgttcctt agaatgttga caaggaaaca
     1861 aaattactat acactatttt tgtttccaag ttaacattat tgcataaagg agtttttaca
     1921 tgtacagttt cacaaataaa cattctaaag tttttggata cgtctctgtc ttattcactg
     1981 gattaggatt tactatcgtc agtcctgtac ttccgttttt aacgctgcct tattctcact
     2041 ccatacatca gcaagctttc tatatcactt tgctcatgtc tgtttatgct ctagctgcct
     2101 tcttatctgc ccccattctc ggtagcttaa gtgatcattt tgggcgtcat ccgatcctta
     2161 ttatcagttt attaggatca agtatcggat atttgctttt tggtttagga aattcgatat
     2221 ggatgttgtt ccttggtcgt atcattgaag gattgacggc aggagaaatc tccaccttat
     2281 atgcttactt tgccgacatc acggaaccta atgaaagaac caaagttttt ggatggatgg
     2341 gtgctttagt cggtatagga acaacgctgg gtcctattat agggggtcta ttagctgagt
     2401 tggggaattc tgttcctata tttattggag cactctttac tttcttaaat gccgtttatg
     2461 gctacacatt tatgctagaa agccttccaa taaaaaaacg ttcagtagat ttaagtttct
     2521 cccatgtaag accttttcac caattacaac aattattcaa gatttcatcc gttattcctt
     2581 tattgacagc aggttttgct gtttggttgg cagctggctc tcttcaatct atcttttctc
     2641 aattttcgat tgacgccttt cagtggaagg ctgggttggt tggtttaagc ttctctttga
     2701 ttggaatctt ggatactgtc tcgcaattgt tcattatgcc tcgattgctc aaaaaatttt
     2761 cagaacagca gatcacaaga atagggatgt tcagtgaaat cctcgcctat ctattcatca
     2821 cattatctgg aattcttcta ctgccgattt tgtttttatt tgggattatc tgttatggtt
     2881 tcggtgattc tatttttact cctgtattca atggccaact atccaattct gtctcagata
     2941 accagcaagg tcttgtaatg ggcggtaccc aaagtataca atctctatcg cgcgtcatcg
     3001 gcccgctaat cgcaggacag ctttatgctg cagcaccatt tcttccgacg gtattcggat
     3061 ttttgcttat gatttttgcc tattatcact acaataaaaa atagtagctt catttgctac
     3121 ttcgtataaa ttctctgtat cgaccgataa aacgaaatac tgtgtcaaat tcttcttttg
     3181 acatattttc aaatacagta gcatctcgct gtacccattt ttgatggagt tgctcgtgtc
     3241 tattccttat tttttgtccg attggcgtca attgaaaata gatttctttt ttattctctt
     3301 cgctttgata agtgtttatg atccctctag ctatcagttt ttttgttagc ttactgatgg
     3361 ctcctctagt catgtaacta gcattcgcta attttgtcac gttaggatca gggattttcc
     3421 cgatatgttc aatcacttct agttcattta gagtaaatcc tgcaaattct ttttccattt
     3481 ctggtttatt acgatgaata atctcattag ctaaatccag atattcttct attagcagtt
     3541 gtcttttatc catttctaca ccttcttttt ctttcattat attgtttttt tgtttcctat
     3601 tcaacaaaga taaaaatatt ttgtaggaaa acccaatctg aaaccaagaa a
//
LOCUS       DACCXW010000187         3650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCXW010000187 DACCXW010000000
VERSION     DACCXW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.195"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(474..773)
                     /locus_tag="IVY71_002752"
     CDS             complement(474..773)
                     /locus_tag="IVY71_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986767.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKGLSPSDVLKEAS"
     gene            complement(878..1081)
                     /locus_tag="IVY71_002753"
     CDS             complement(878..1081)
                     /locus_tag="IVY71_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986768.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1096..1842)
                     /locus_tag="IVY71_002754"
     CDS             complement(1096..1842)
                     /locus_tag="IVY71_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7986769.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            1920..2228
                     /locus_tag="IVY71_002755"
     CDS             1920..2228
                     /locus_tag="IVY71_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986770.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(2199..2456)
                     /locus_tag="IVY71_002756"
     CDS             complement(2199..2456)
                     /locus_tag="IVY71_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP7986771.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            3188..3508
                     /locus_tag="IVY71_002757"
     CDS             3188..3508
                     /locus_tag="IVY71_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986772.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3526..>3650
                     /locus_tag="IVY71_002758"
     CDS             3526..>3650
                     /locus_tag="IVY71_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP7986773.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
BASE COUNT         1083 a          642 c          619 g         1306 t
ORIGIN      
        1 gtatacaagg agattaaaaa taaagagttt actgataaag atatgatttt catgggtttt
       61 ttagtcaaaa ttctttttat attttccata gtaatcactt tattattttg tttaaatgaa
      121 acacaatttt ctgtagcgac cactttgatt tcatttattg ctattttttc ctttatattt
      181 tccgataatc agaaaaaaag tattaacgga tttctgcaaa aaataaagaa aaaaatcaat
      241 ggagaaccct aatataaaaa tcaggaaaaa aatattgatt actcctctta ggaagaccat
      301 tagtcagtcc ctcccggctg gctttttcgc tctgtactca gcttcatcaa gccccataaa
      361 aatccagacc atatatacga ttgtgccgat taatgcttgt ctgcttcccc aaagacctaa
      421 agcgtagatg atgaatggtg tgctgaatac tagcgctctg ttgaatttgc ccattagctt
      481 gcctccttca aaacatctga aggcgataaa ccttttacac gatatttgtt tgcgtctttc
      541 cactttaaaa accaaataaa agtatggtaa tgaataaatg tggttgagtg acctggacga
      601 agtattcctt ctgcaaattc aggaattgtt tccatttcct tgcagtattc aactaaagta
      661 gtttttgaca tgccgtggaa cttctccaag attaaacttt gtcggtacca gtcgtctggt
      721 tttactgcct tctcagcagc ttctatcagc tctgaaagtg ttggtttttt cacttattat
      781 ccctcctaaa aattatcgtt ccatactcga tgtttaacat ttcttgaagc tctgattttg
      841 taagaatgat tttatcttcc aacgacttta cctccctcta caattcgtac atagtgataa
      901 tcgaatctat aattctgttt gcttctgcag aagtcttttt accgtttaaa attaaagata
      961 agtagctttt gctaattcca aatctttcag caagcatggt gtaagttaag aactttgaac
     1021 tttcgacata ttctttgatt ttttctctat ctcgttgagt gatttctgca atgtcagtca
     1081 tactaaaact cctttctaac caatttcctc taaatccatt tgagggtaat agccctctgc
     1141 gattaataag ttgtaaataa acactcgccc tttctgtgtc catttggtat tcattacaac
     1201 tttagtgccg ccatcggctt tcggtatctc acttgtgtgc gattttgtgt atccttggtt
     1261 catatgtttt cggtaaagta tccattgtcc gctaacttta tgctgaacac ctaactcgtt
     1321 tagcaatttg tttagagcaa ttgccgacat tccgtaatca gctgcaattt gagaagtagc
     1381 taccgtatct gtcgatgaaa gaatcgtatc taagtagcta atctttggtt cgtactcggc
     1441 aatttgttgt tcgagtaatt ggttcttttc ttctaaatcc gctgctaaac gcaatgcttg
     1501 tgcaaaactt tgaggtacgt ttgaatagct acctgttttt ctaattgttg gaaggacttc
     1561 actcgttacc caacgtttaa attttttggc agagggaagt tttgatttta agattaaact
     1621 gtaaaggcct gactcgttaa tcaaaacaac tgctgttcca ttgacggtga acgaatcgtt
     1681 ctgcgtctta tcctccaagt ccacatgatc tcgaatagct ttttgcggat tcgaatatcc
     1741 tagaacgtct gctacatctt ttcctacaaa atatggttca tcatttacta aaatagttcg
     1801 aacttcgttt tgctcgaaat taaaaatttg tggtgtattc atatttctca ttcctttctt
     1861 tggtataatt tccttatcag tgtgacaggc tgaaataatt gttaaggagg tgaaaattta
     1921 tgaaaccaga atttgtaatt actatgcttg atggagaaaa aatcaaaata tttgccgata
     1981 ctttggtcgt tggatacgat gatgcgtcaa atatcgaaga aaaagatgga atgttctatt
     2041 cttctcaaat ttacgtgaat accttgcaag gtgatattgg ggtatctact cttgcaacat
     2101 caagaccgct tgtaggtctt atcggctttt tgtcaaaagc aacattcttt tctctcggcg
     2161 atgatataga ttcggatgaa actgtcatct ataaaacatc agctgttaaa agtatcggtt
     2221 tcaaatagta attcttgctc gttgatagcg gaagccaact tttcttccaa cacatcggcg
     2281 agctttcttt ttgttggatt catttcaaag ttgatagtct tgagaagcat ttttgcatcc
     2341 ttaacttttt gtccttgcaa ttccttgatg attttatcgg cttggctatc aattccatgt
     2401 gttagatcat ctatcatttt ttgtaccttg attgtttggc tcatttcctt gttcattgtt
     2461 tagcctcctt taataaatga ttttttgtat tttttaattt ccataaattc ctcaaattgc
     2521 tctttggtat tattaccacg accatacata ttgtgaaatt ctttatggca actgacacaa
     2581 agtgtaacgc cgtttgaaat cgctgcggat ttagttgttg catggctaaa tcacgaatca
     2641 caagtatcta ctaaaggcga atcaatttcg ccaaaagaaa ttgttcaagc ttatttagat
     2701 attcattatg ctgttatcta tggtcagctt ccagaagacc gtaaaaacga tgactaaacg
     2761 tgagctaaaa tctctgctat ggctgcaacc atggcagagt cttctttatc taccgcgtgt
     2821 tccatcgcca cttttgcttg tttcagtatg tctaatttca aatcttcgat ttttgccgag
     2881 actgtttttt ccatattctt ctctcctttc ttttatattc gtaaacaaat ttaacaactt
     2941 tttaaaaaac tgcgttgact aaattaacat acagtgttaa aatcagaaca tagttaaata
     3001 agacatataa acattgattt taaaagcttt cttggcggtt ggcatttatt aatcaatagt
     3061 gttttttgtt gtctttttag ttgttaaact tgtttacaag aatgatatta acaccttttg
     3121 ttaaaatagt caacgaattt taacaaataa agttaaatat tttttcttga acgaaaggaa
     3181 tattgatatg ataacgtttg agagaataaa agagttagcg aaaaaacagg gaaaatcatt
     3241 aaataaagtt gaagaagacc taggctatgg taaaaacgtc ttatatagac ttaagaactc
     3301 aaacccctct acagaacgtt tgcaagaaat tgctgactat ttcgatgtct ctgtagacta
     3361 tcttctgggg cgcgaagaaa aagaaacccc taaacatgtg gatttatcag aaaacgatac
     3421 tgttttttct tttgatggaa aagaaatatc taaggagaca atgcgtaaag cgattgcaat
     3481 tgctaaagct ttagaggaaa atgaatagtt ggagtgatgg gttgtatgta tttgaaattg
     3541 aaagaaatgt tgagcgaata caatttgaaa gtagtttaca tggaaatgaa agagcctggc
     3601 ttctattatc cgaagcctag aatcattttt ttgaatgaaa atctatatgg
//
LOCUS       DACCXW010000188         3649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCXW010000188 DACCXW010000000
VERSION     DACCXW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.196"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            57..197
                     /locus_tag="IVY71_002759"
     CDS             57..197
                     /locus_tag="IVY71_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7986774.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAV
                     VN"
     gene            complement(534..950)
                     /locus_tag="IVY71_002760"
     CDS             complement(534..950)
                     /locus_tag="IVY71_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986775.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(952..1233)
                     /locus_tag="IVY71_002761"
     CDS             complement(952..1233)
                     /locus_tag="IVY71_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986776.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1957..2361
                     /locus_tag="IVY71_002762"
     CDS             1957..2361
                     /locus_tag="IVY71_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP7986777.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            2378..3526
                     /gene="tnpB"
                     /locus_tag="IVY71_002763"
     CDS             2378..3526
                     /gene="tnpB"
                     /locus_tag="IVY71_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7986778.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT         1196 a          554 c          684 g         1215 t
ORIGIN      
        1 aaacgagaat tagatacatg gcttcaagga cttgatgtga cggaagtagg tgctgaatga
       61 gtgattcgat agaaaaaatg accctaaaaa aacttgctga tgaattgggt gtatcgaaaa
      121 atacggtgcg caatcaactg gtcaattcgg gatttgacct tgctcagtat aaagaaaaaa
      181 atgcagtggt caattgaccg aaatttatgt ctttgggttt tggggtttta aaacgaaaaa
      241 aagggggcga ttttcgcccc ctttgctcta tgtgaaaaat ttgttatact tattttatcc
      301 ttaaaacata ccaatctttt tcttatttag ctgctcgtac gagcagcttt ttttacgatt
      361 atgaattcaa atcatgtatt tataattttt ttgtttttat ctttttttct gttatttctt
      421 ctgaaattcg tttttttcat tcaattggtt actatcttat tgtaaatgtt gctattaaga
      481 tggtgatcct acccaaaaaa taaaccagga atttaaatct cctggtttat tttttatctt
      541 tttagaaaat ttgttgggtc gaaattttta gaagtaaatg tttgattggt ttctttttga
      601 cgattttttg gttgatttcg tttggaggga ttaagttgtt tttcaatttt ttctttcgtt
      661 tcttgataca ctttagaatt tgtgccaata agaatacctg tatcttttag atataaatca
      721 gcaaattctg caatgatttc ggacatgatg gaaatttctt ttcccatagc atttagtttg
      781 gcttggttgc tggtagtcgt agtattggta tcaggttcat aactcataca taaaaaacga
      841 atggcttccg agtaattgtt gatatctgga tttctttcga taatggaatc taaaacgtcc
      901 acgtgactgg gataaagggt aacgtgtttt tgaacagaag tttttctcat gttatcttcc
      961 tccgataagt gtttcaatga gttggttgtt tgtacgaaca tattcattca gcgtttcagt
     1021 atttttttct tgaatagcga taaatttttc gagtaataaa tcgaattgat ctttcttgta
     1081 atgcataagt gcgtggtcaa aatcgttttc gattaggact tttaagtagc gatttcgact
     1141 ccattttttg ccagttttat ttgtgagttc ttggcaacgt ttgtctattt ttgcaataac
     1201 ctttgaatcg atcttccgaa ctaaaatgtc catagaaaaa ctcctttcgc atttgtgtat
     1261 aatttatgta tatcatacga atatagtatt tgtatagctg ttttttgcta aaatactata
     1321 cataagctag ccacgatggc gaaaaagtga tatcaaattt ttttctgaga aacgtgatag
     1381 gcacagccta tctacccact ttttttagaa agtgggtaca cgtttcctta tgctctttat
     1441 aaaatggtga tgtgcgactt ttttctgttc gtaaaaataa aaaaagttta cgaaaatgaa
     1501 actaaactga acgattatga cgggcatgta atcaagatga aatgatttta taagcgcgta
     1561 ttttgcgttc taagacgttt tagcgttcat ttcgtttagt tatcggcaca atcgttaaaa
     1621 cacgtaagag aagcgagaat ggagcctgta aggcgactgg tagcgaaacg gcgtgttaga
     1681 ttgtgcaagc cgagaactga atgaaatgaa gagcggaagc gtgaaataat ttcggcagga
     1741 ggaggctcaa ggggttcggg gaatgaaatt ccctgaggtc tttttatttt tttaacagaa
     1801 ttaggcagaa gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg tatgagggtt
     1861 accgttggta actcgaagac cgacaactaa ggcgtattgc ctttttattt tgttccatgt
     1921 tacaatcagt ctatacaaga aatgagttga tataacatgg aattagatac aaataatcat
     1981 tcagtatttc ttctttatta ccaccttgtt ttagtaacga aatatcgtag acaagtgatt
     2041 gatgatgaaa tatctgatta tgccaaaact acttttgaaa gaatttcaga atcgtatcac
     2101 attactttag tggagtggaa tcatgataaa gaccacgttc atattatgtt caaagctcaa
     2161 cctaaaacag aattgacaaa attcatcaat gcctataaaa gtgccagctc aagattgata
     2221 aaacgtgact ttccaagagt caaacaattt ctttggaaag aaatgttctg gtctaaaagt
     2281 ttttgtcttc tgacaactgg tggcgcacca attgatgtga ttaagaagta tatccaaaat
     2341 caaggaaaca atcataaata gaaagtaggt gaactttatg gaacaactaa aagcatacaa
     2401 attcagaatc tatccaacag aggaacaaga aatcttcttt gccaaatctt ttggttgtgt
     2461 ccgtaaggtc tacaatctaa tgcttgatga ccgaaaaaag gcgtatgaag aagttaaaaa
     2521 tgattcttct aaaaaaatga cttttccgac accagctaaa tacaagaaag aatttccgtt
     2581 tttgaaagaa attgatagcc ttgctttagc caatgcccaa cttaatttag ataaagcata
     2641 caagaatttt tttcgggata aatccgttgg atttccacgt ttcaaaagta agaaaaatcc
     2701 tgttcagagt tatacaacga ataatcaaaa tggtacagtt gctttgattg atagtaaatt
     2761 catcaaagtt cctaaattga agtcattagt aagaattaag cttcatagac aaccaaaagg
     2821 gattatcaaa tctgctacaa tatcacgtca ttctagcggt aaatactata tttctttgtt
     2881 gtgtaaagaa gaaattatcg aattacctaa aactaattcc acaattggta ttgacttagg
     2941 tattatggac tttgcgattc tttctgatgg acaaaaaatt gataataata aattcacgtc
     3001 caaaatggaa aagaaactaa agcgtgaaca acgcaaattg tctaggcgtg ctttattggc
     3061 taaaaataaa ggtaccaatc tatttgaagc taaaaattat caaaaacaaa aacgcaaagt
     3121 agctagattg catgaaaaag taatgaatca acgtactgat tttctgaata agttgagtac
     3181 agaaatgatc aaaaatcacg atattatctg tattgaagac ttaaacacaa aaggtatgtt
     3241 gcgaaatcat aaattagcta aaagtatttc tgatgtttct tggtcaaagt ttgtgactaa
     3301 attacaatac aaggctgact ggtatggacg aaaaatcatc aaagtagata aatggtttcc
     3361 gtctagtcag atttgttcag agtgcggaca taaagatggc aagaaatctc ttgaaattcg
     3421 agaatggact tgtcctgttt gtcatactca tcatgatcgt gatatcaatg ctagtatcaa
     3481 tattctgacc gaaggtctaa gactacattc tatgggattg gcttagatcc aatcatcaag
     3541 aaccgtagga actacgggga tagcttggta aataagagac accgctgtta gtaaagaaat
     3601 acgctatcaa gtatgctcta ttcccaagaa gctcccactt caagcgtta
//
LOCUS       DACCXW010000189         3648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCXW010000189 DACCXW010000000
VERSION     DACCXW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.197"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..478
                     /locus_tag="IVY71_002764"
     CDS             <1..478
                     /locus_tag="IVY71_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7986779.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="IVY71_002765"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="IVY71_002765"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7986780.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="IVY71_002766"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="IVY71_002766"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7986781.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..>3648
                     /locus_tag="IVY71_002767"
     CDS             3200..>3648
                     /locus_tag="IVY71_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7986782.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDL"
BASE COUNT         1189 a          588 c          799 g         1072 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
     3601 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatct
//
LOCUS       DACCXW010000190         3575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCXW010000190 DACCXW010000000
VERSION     DACCXW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.198"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..196
                     /locus_tag="IVY71_002768"
     CDS             <1..196
                     /locus_tag="IVY71_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7986783.1"
                     /translation="KNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLGLVPVE
                     SVEEVLGMKIEVEEDFQNHG"
     gene            189..338
                     /locus_tag="IVY71_002769"
     CDS             189..338
                     /locus_tag="IVY71_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP7986784.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            373..1157
                     /locus_tag="IVY71_002770"
                     /pseudo
     CDS             373..1157
                     /locus_tag="IVY71_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1441..2526)
                     /gene="xerS"
                     /locus_tag="IVY71_002771"
     CDS             complement(1441..2526)
                     /gene="xerS"
                     /locus_tag="IVY71_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7986785.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2860..3093
                     /locus_tag="IVY71_002772"
     CDS             2860..3093
                     /locus_tag="IVY71_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP7986786.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            3090..3497
                     /locus_tag="IVY71_002773"
     CDS             3090..3497
                     /locus_tag="IVY71_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7986787.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         1257 a          498 c          633 g         1187 t
ORIGIN      
        1 aaaaaatgaa ttaatgaaat taggttatgg gaagtatcag tctgagagaa tcatacgaca
       61 ggcaaaagca attatggctt caaaaagcta tacctattac gaaaatagaa gattaggact
      121 agtacctgtt gaatctgtag aagaagtttt aggtatgaag atagaggtag aggaggattt
      181 tcagaatcat ggttaatttt aaaaaagata aaaatgggaa atatcatttt actgctagtt
      241 tgggattcga caaacgaaca gggaaaagag tacaaaaaaa cgtagagggg ttaaaacagt
      301 taaagaagaa aaaaagcgta taccgaactt gtttctaatt atggaaaaga agcagaaact
      361 tctcaaagta ctatgttgtt tgaggagttt ttttataaat tattcctacc ttattatcaa
      421 gggaaagtag aagaaagaac atataaaaat cggttatcta caatcaaaaa aacttttctt
      481 atttctttaa aatggagtta agagaaatac gtccaattga cattcaaaag tggcaaaata
      541 atttattgag caaatataaa aattcttatg taagaaacat gtatgggatt tttcagatgt
      601 cattagatcg agcagttgtt ttgggactac ttgaaaaaaa tcctgcaaaa gttattggaa
      661 atgtgaagaa aaggaaaact acagttgatt tttggacaaa agaagaattt acaaaagtta
      721 tttcaacttt ttatattgat gatttgtacc aacattttgg ttttatttgt atgtggttac
      781 tttttatgac cgttatgcgt ttgggagaag ccactgcatt aacttggaaa gatattgatt
      841 ttgaaaataa aaacttgcgt gtgaataaaa cactctatta taagagagcc aaaacctatg
      901 agtttgtaga acccgaaacg gcttctagta ttcgtacaat cgaactagac gacaatacaa
      961 ttaccttttt aaaagcttgg aaggagcgtc aagtttcaat aggaggcatg tcgtttgtac
     1021 tttcttataa tggaacacct actcagaaat atacaatccg tcatattatg agaagacatg
     1081 caacgatcgt tggtgttcat ataatccgca tacacggatt aaggcatagt catgcttctt
     1141 tgcttatatc acaataaaaa gatgcacttc gaattaaaga aaggttaaaa catgctgatg
     1201 tacaaacgac tttaggaact tatggtcatt tgtatctaaa ttcattgaga gagataacaa
     1261 atgatcccac agattatatc caaactaatt tttctaaaat caaaccgaca cctatttacg
     1321 ataataaaaa ataaatgata aacaaaagta ggatcacacc aaaagaaaca cactagaaaa
     1381 actataatac aggcttttct agcgtgttgt ttcttatttc cgcctattta tacattatac
     1441 tcataaagca tttaatgcat cgatcgtagc cgcattgtct atatgggtat ataaagttgt
     1501 tgtattggta cttgaatgtc ccaattgttg ggcagttaat accaatgaat ttgtctgttg
     1561 gtagagtcta gtagctaatg tatgacgcag tttgtgaggg ctgacttgaa ttctaaagcc
     1621 ttttgaatat tttgctacca tccgttcgat tgcaataccg gatatacgct tggcttctcc
     1681 ttggtaaatt gctagaaata aagctttttc gttttctgtt actttatatc gttctttccg
     1741 aatagctaga taattagcta aatattccat tgcgaatgct gctatattga cagcatctct
     1801 tttatttcct tttctagtta ctaccacatt attcttatct aaagataggt catctaaatt
     1861 aatatttata acctcggaga gtctcaatcc agatcctaaa atcagtgcat tgatcgccag
     1921 gtctctttct acatctcgtt gataatagat caaagctcgt ttactcaaag tatttttata
     1981 ttcattggca atataatcta gataattttg tgtatcctta tctagaaata attttttctc
     2041 aatggcattg gcacgtgcag ctaacgtagt tttatctgtg atggttgcta cttgatccat
     2101 aggattattg tctaagtatg gttttcctag aggattattg gaacttttcg aaagatattt
     2161 aaatagaacc tttaatgccg tgatatttct attgatagta gaagtagata gacctaagtt
     2221 attattatct ttgagcttag tttttgttgt ctcttttttt acattttctt ttgtgcgacg
     2281 tttcaaatga gatttgtaca agtttaggtc ataggcagtt aactgtctta tcgaggtgat
     2341 agaagtatca ggaacgatag actcactcag aagccattca taaaagagct tataattttt
     2401 tagatattct aaagtggttc gaggtgattg atctttatcc tcacgatctt gaacaaaatc
     2461 ttttatataa tcgggcatat gaagtatgat tgtatctatt ttttgatata tttctgtatt
     2521 tttcatgtca ttcatccttt caataagtag gggagaagag tatctctata attggactat
     2581 accaattata gaatgttttg tcaattggta tgctctaaaa tttattaatt tgtttcaaaa
     2641 aaagcatata aacaggtata cgtttaataa tttaaaataa atggtataca taaataggga
     2701 aggaaataga agggagcaat ctaaattttt tgaattttct taattaatta tatagaaaga
     2761 acaatctttc tatagagtta gacatgagag ggactatgct tgatttaaaa tcaacaagcg
     2821 tatatactgt atatacaaag aaaaaggagg gagaagacta tggcaattaa agaaagaaaa
     2881 tttagggaag tggggaactc agtagtatta actatttcaa aggagtttct taaagaaaat
     2941 gggttaaatc ccggagatac ggttttttta gatgaagaaa aacttcgagc ggcaattaca
     3001 aaaaaacaag ccttatctga tgaaattgat ttagcaatat ctcaagcatt aactgaatat
     3061 gatactactt ttagagagct tgtcgataaa tgagtattca ttacctagaa gccaaagata
     3121 ttataaaaat gaatgtaatc caaataaaaa aatactctcc taatgaaccc cttggtgtaa
     3181 aggatgcgaa tgctttagaa atgtgtgtag ggcaacttcg agcctcagct tttggtgaag
     3241 aggtttatcc ttcgatttat gaaaaagcag ctatcctact tatacaattg ataaagaaac
     3301 atccttttca caatgcaaat aaacgtactg ctttcttagc tacttttgtc tttttgaaaa
     3361 tcaatggaca gcttttagct attgatcaaa aagatgctgt aaatttagcg gtatatattg
     3421 ctacgtatga taaggatttc gatcaactaa aatatgaagt gatcaacact ataaagacac
     3481 atacgcagcc catttaagga gttactgtat gtgtctttat agggttatta gctgctatag
     3541 cgaaataatt gaagtaaatt acaaatagct ataat
//
LOCUS       DACCXW010000191         3502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCXW010000191 DACCXW010000000
VERSION     DACCXW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.199"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..642
                     /locus_tag="IVY71_002774"
     CDS             <1..642
                     /locus_tag="IVY71_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7986788.1"
                     /translation="KNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRV
                     KEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSP
                     SRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQN
                     ISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            632..1372
                     /locus_tag="IVY71_002775"
     CDS             632..1372
                     /locus_tag="IVY71_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7986789.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1369..>3502
                     /locus_tag="IVY71_002776"
     CDS             1369..>3502
                     /locus_tag="IVY71_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986790.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWM"
BASE COUNT         1299 a          433 c          688 g         1082 t
ORIGIN      
        1 aaaaatacca tgattaatac atggaataac attaaggact ctttctggaa tattgttact
       61 ggaattgtta attctgctga aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct
      121 attaatcggg taaaagaaat ctttaattcg ttaagggaag tcaatttatt tgaaattggc
      181 aagaacatca ttgatggact tataaatggt gtaaaagaaa agtggaatgc tttgaaagat
      241 actatcaaag gtattgcagg taatatcaaa aattggatta aaggagcttt agatattcat
      301 tctccatcaa gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt
      361 ggtattgata aagagcaaag taagttagat caaaccatga caaatttagt aaaaacaccg
      421 tctgttcaac ctgtagtaaa tggaataagt cacctaccaa ttacacaaac aaagcaaaat
      481 atatctgcta gtgaaacaaa agaaattcat ttacatttaa atgtttatgg agatttacct
      541 gattcaatgg ttcgaaagat tactacaaaa attaaaacag aattgacaag acaaatgaaa
      601 cgagatgctg gtgcagtagg aggaacatta tatgcaactt aagagaggac aattttttat
      661 taatcaacat tattcttctg aatttaatgt gtatattcaa aacaggcctg cttctgtttc
      721 agctagtcga gtaattgaat tgagagagcg agaaggaaat gattctatca ttattgataa
      781 aggctattac aaaaatgtga ctagaaagat tgagtgttac tataaagcac cttcaattga
      841 tatggtgcag gagtgggaag atcgaatcac tgaatggtta gatatgtcgt cttatagtga
      901 ttttattctt tactatgatg aacaatatat ttatcaagct gtagtaatag aaggacctga
      961 atttaaagga acaagaaaaa caggaaatat tgtccctttt gaatttacag tgagtattcg
     1021 accatttaag gaaaattaca atggtagatt tactattcga caaacagaaa cttttgaaat
     1081 ttataaccca gagaagtatt cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc
     1141 ttctttttat attaataagg ataaatatga tttgaaatcg ttggacagag aattatttat
     1201 agattctaaa ttagaagagg cttatcgaaa attagatggt aatttagaac atcaggatca
     1261 ggtcacctta tttttagatt ttccattttt atatcctgga aaaaatgaaa ttaaatggac
     1321 gaaaaatatt cattcatttg aaataatgcc taggtggtgg agaaaagtat gaaaccaaga
     1381 atatatagtc ctactgaaac agatttctca acgaatggct taggaatttt aaaggattgt
     1441 acaagatgtg aaatatatga agtagctaat ggaaaatatg aattagagtt ggattatcct
     1501 ttaggaacta gatttgatga atattttgaa aatgactatc aaataaaagc aaagccaaac
     1561 gatcaagaag agtatcatat cttttttatt gatgataaag atatagatac ttttttaaat
     1621 acagtaacta tttatgctca gagtcgtaca aatcgacttg gaagacgggt agtcactctt
     1681 gtggaagtgg actctaaaac tggtcaagaa gcgatatcaa ttattgaaac taaaatggat
     1741 aaaaaatctg acatacgact ttattctgat attacggctg tttctagcac aatctttgaa
     1801 gcgagaaatg ttttaaattg tattgctggt gaacaaggat cattgcttca atattggggc
     1861 ggagaaatta aacgtgaacc atttaagctt tctttgttaa agcgaagagg tcgcaataat
     1921 gttggaacga ttcggtatgg aaaagatatg tctggtttga aggtcaaatt agattggaca
     1981 ggtataaaaa caagaattat tccatatgct gatcctcaaa gtgaagtagg tacgactaat
     2041 cgaatttatg gttcgccagt agatagtgcc tatattaata actatcctga tgtctataca
     2101 gaacatgttc agtttacaga agaacaagga gtaaaagaca ttaagagctt aaataaaata
     2161 gctaaaaatt actttaaaac gatcaatcct ggctgtgata agcctaaagt ttctattacg
     2221 gttgaatttg ataagttgac tgatagtgaa gaagcgaaag aatttgcgaa gattagaaat
     2281 tatggtctgt ttgatacatt taaaatatat cataaaaaat ataatattta tcttgaatca
     2341 aaggttagtg gactacaata cgattcttta tctgaaaaaa ctttgaaatt agaagcagga
     2401 gacattcaag ttgcttttta tcagcaacaa gctgtaacta ttcaggataa attaaaagat
     2461 tatgcaacga ataattatat gagtgatttt aatgattatg tttcttcaat gattacaggt
     2521 caaggcagtg caggaggata tgtagtttta tggccaaaag aaaaaccttc caacatcttt
     2581 attatggata atccagattt agaaaaggca agagaagtac taagaatgaa taaaaatggg
     2641 attgcttttt ctaagaaagg atggcggggg ccttttaatt cagcttggac attagatagt
     2701 atatttaatg ccaattttat acaaactgga ttaatcaaag cagatatctt tcaaaattct
     2761 tttaacaaga ctggagatgt attgaaatta gttaatggac tactccaaat ttggaacaac
     2821 aaaaagaaaa taatggagtt aactaaaaaa ggaatggaat tttggaattc taagggttca
     2881 attggaacga ttggaacaac tgattctgtt ggtaatcctt ttcctggggc ttctactcca
     2941 acccctattg aagataattc tttagttatt cgtacaaatg gagacggcaa atatattttg
     3001 atttctccta aagtaggaaa aggattagtt ttattaggaa atggtaaagc aatttatttt
     3061 ggagacttag atgtacaagg aaaacttaca gttaaaggaa aagaaataat tcctggacaa
     3121 ggtggtggtc catcgggagg tggagaatct actggtgggt atcctagtga attaaaaaca
     3181 gatgcagaaa agagagcttg gagaatttac gatattttgt gtaataacgg atttacgaaa
     3241 caatctgcat gtggaatatt ggggaacatt caacaagaaa caggaggaac ttttgatcct
     3301 gatactgttc aaataggtgg accagcatac ggattagttc agtgggatgg ttcttcatat
     3361 cctcttgttg gtccagcaac atgggatgga aaagtttatg ttcaaaactt atttaatgct
     3421 gcaggtatta aagaaccaat aacgagctta gatgcacaag ttcgattgct tatttggaca
     3481 tttacaaatg gacaatggat gg
//
LOCUS       DACCXW010000192         3336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCXW010000192 DACCXW010000000
VERSION     DACCXW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.200"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(451..1761)
                     /locus_tag="IVY71_002777"
     CDS             complement(451..1761)
                     /locus_tag="IVY71_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7986791.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IVY71_002778"
     CDS             complement(1773..3191)
                     /locus_tag="IVY71_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7986792.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1033 a          676 c          515 g         1112 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataaga
//
LOCUS       DACCXW010000193         3210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCXW010000193 DACCXW010000000
VERSION     DACCXW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.201"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..512)
                     /locus_tag="IVY71_002779"
     CDS             complement(<1..512)
                     /locus_tag="IVY71_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986793.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRI"
     gene            complement(611..961)
                     /locus_tag="IVY71_002780"
     CDS             complement(611..961)
                     /locus_tag="IVY71_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7986794.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1118..2647)
                     /gene="ahpF"
                     /locus_tag="IVY71_002781"
     CDS             complement(1118..2647)
                     /gene="ahpF"
                     /locus_tag="IVY71_002781"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7986795.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2657..>3210)
                     /gene="ahpC"
                     /locus_tag="IVY71_002782"
     CDS             complement(2657..>3210)
                     /gene="ahpC"
                     /locus_tag="IVY71_002782"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7986796.1"
                     /translation="GKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFTFVCP
                     TELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSHQISR
                     IFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRTHPGE
                     VCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         1005 a          666 c          540 g          999 t
ORIGIN      
        1 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
       61 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
      121 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
      181 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
      241 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
      301 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
      361 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
      421 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
      481 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
      541 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
      601 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
      661 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
      721 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
      781 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
      841 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
      901 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
      961 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     1021 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     1081 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     1141 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     1201 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     1261 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     1321 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     1381 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     1441 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     1501 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     1561 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     1621 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     1681 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     1741 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     1801 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     1861 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     1921 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     1981 ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt tctttttcaa
     2041 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     2101 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     2161 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     2221 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     2281 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     2341 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     2401 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     2461 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     2521 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     2581 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     2641 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     2701 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     2761 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     2821 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     2881 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     2941 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     3001 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     3061 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     3121 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     3181 gtgcattaaa ttcttcgatt tttttcccaa
//
LOCUS       DACCXW010000194         3152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCXW010000194 DACCXW010000000
VERSION     DACCXW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.202"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            5..1012
                     /locus_tag="IVY71_002783"
     CDS             5..1012
                     /locus_tag="IVY71_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7986797.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1024..2028
                     /gene="neuB"
                     /locus_tag="IVY71_002784"
     CDS             1024..2028
                     /gene="neuB"
                     /locus_tag="IVY71_002784"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP7986798.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2025..>3152
                     /gene="neuC"
                     /locus_tag="IVY71_002785"
     CDS             2025..>3152
                     /gene="neuC"
                     /locus_tag="IVY71_002785"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP7986799.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWI"
BASE COUNT         1193 a          405 c          603 g          951 t
ORIGIN      
        1 ataagtgttt aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga
       61 aattgttcgt cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga
      121 agaaaaacaa ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat
      181 tggtgatatc agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt
      241 tcatgctgcc gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa
      301 aacaaatgtg atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca
      361 ttttgtaggt atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa
      421 gttattgagt gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat
      481 attttcttgt gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt
      541 tagtaaacaa ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt
      601 tatgatgtca ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt
      661 tggagaaaca tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt
      721 aataaaaata tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg
      781 tatcaggcca ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc
      841 atatgaaaat gataaaatgt atatcattcc accacactat catgctactg acgaactgta
      901 caaagattat gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag
      961 tggacaggaa tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt
     1021 atgatgaaat cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta
     1081 gatttagctt tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt
     1141 caaacattta aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag
     1201 gataatctta aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa
     1261 tctgatttta ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact
     1321 ccttttgatg aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt
     1381 ggctcaggag atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca
     1441 gttttattat ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc
     1501 atagatagtc cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat
     1561 attaatttat tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc
     1621 gatcattcat tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt
     1681 gagaaacatt ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat
     1741 cctcaggaat ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat
     1801 ggtgttaagc gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta
     1861 gtggtcactg agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa
     1921 cgtcctggaa atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa
     1981 agaccaataa gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta
     2041 tagtaagtgg aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac
     2101 aagataattg ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat
     2161 ttactaaaga attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt
     2221 ttttagagaa caataaggga aacatggctc ggtctgtggc attagagata aatggtttca
     2281 caaatatatt tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa
     2341 tgttggcagc tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag
     2401 gcgaagtgtc tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg
     2461 ttcattttgc tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt
     2521 ggagagtttt tcccgtcgga gctcctagaa tagatactat tttaaataat agattaccta
     2581 gttttagaga tataaaagaa aaatatcatc taaaatttga aaagaaaaaa tacgttttgc
     2641 aagtatttca tccagttgta accgaactag aaaatataga aaaacaaatt ttagctttga
     2701 tcaattcaat ggaacaacaa tctcaaccaa ttatttgcat tttacctaat agtgatgctg
     2761 gtagcgaaat tatcagaaaa atgtaccaga aatatttaaa aacggatatt atttatatta
     2821 cgaatttaga gccggaggaa tacctgacta tattaaaaga gtgcaaattt atgatcggaa
     2881 attcttcttc tgggataatt gaagctgcat cattcaaaat accagtaata aatttaggaa
     2941 ccagacaaaa tggacgagaa caatctaaaa atattataaa tgcaaatcta aatgaaaatg
     3001 aaattgaaac tgctttgaat aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg
     3061 ttaagaacat ctacggtgac ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa
     3121 tattgaatga tggggaagat attaagtgga tt
//
LOCUS       DACCXW010000195         3123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCXW010000195 DACCXW010000000
VERSION     DACCXW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.203"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(272..640)
                     /locus_tag="IVY71_002786"
     CDS             complement(272..640)
                     /locus_tag="IVY71_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7986800.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            790..1272
                     /locus_tag="IVY71_002787"
     CDS             790..1272
                     /locus_tag="IVY71_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7986801.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1309..2319
                     /locus_tag="IVY71_002788"
     CDS             1309..2319
                     /locus_tag="IVY71_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7986802.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2434..3120)
                     /locus_tag="IVY71_002789"
     CDS             complement(2434..3120)
                     /locus_tag="IVY71_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986803.1"
                     /translation="MVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAEKSSTQKSAS
                     TETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLAGYSDLQIEY
                     ARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVMLTGKYSYQGV
                     LVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAIPTGNPNDVK
                     QLIDVTVIDN"
BASE COUNT         1052 a          498 c          624 g          949 t
ORIGIN      
        1 attatgaaag atcactgata ttttctcgta ttttttcctt attcaaaagt tttgcaggaa
       61 aattgctgaa aaaataattt tactaagtag aaaattcaaa gccattcaat cagtgatttt
      121 tttcagcaca agaaagactc cttataaaaa tagcagatac tttgtcaatt aagtgtttaa
      181 ccagggatga taaaaaggat aggattgaac aaaacctgta taaggtgatg aatagtttcc
      241 tcgccagtaa aaggtttatt cgtataaagc tttatcgcca aaatttatca tgaccttttt
      301 tatcttcaat attaaatgaa atcaattgtt caatcaattc ccagttgatt acacttttcc
      361 aaggaacttt gatgacgtta tccgtatata cgtagcctgc ttcttggata tttttttcaa
      421 gggcacgaat cgctgctttt tccggtgcga tagaaaaatg atttttagca aaactaaatg
      481 caataataaa ggtaccatgg tcagtaaaca taggttggtt ccattttaca gttgtgtcta
      541 gctggggata cgtttctgtt atcttagcaa aaaggtcttt gatcttttgt tgatgttctt
      601 tttctttgat ttgattgatg atcggctgaa atttttccat tctaaaacgc ctccattaag
      661 tggtttgatt tattttacca gaaaaacaat agttatgtat aaaaaacgaa caaaacacaa
      721 gtaatgttgt aaccgctata tttatatgat aagatgtctc aaataataaa atagaaaaga
      781 gacataaaaa tgattgactt ggcaaagaaa ttcttacacg aaaatatata ttttgagttg
      841 tatcatcatc gagccattta tacaaatgaa gatgctttgg taataaagaa agaacaagga
      901 tttacaggta cagagaccaa aagcttattc ttaaaagata aaaaagataa taactacgta
      961 tatctgactt ttactacgaa acaaacggat ttcaaagcat tagggaagat cgtcggtaaa
     1021 aggctttcta ttgtttcatc agaacgcatg gaagaacaaa cgggacaaaa gcagggcgct
     1081 gtttctccgt ttggttatga aaaacagatt ccgattatta ttgatgaaga gctcttatca
     1141 aatgaaaaac ttgtttttgc accaggtaga ccagatcaaa cgatggtcat ccaaacggtt
     1201 gacttaaaca aaatcatcca gctacttgag atgccgatat ttttgatgcc ggcaaacgaa
     1261 aaaaacaatt aaccaagtaa aatagtgcat acttggaggt gaagcaacat gataagttat
     1321 ccattattag aaagaaatgc aacgattggg gttactgcac catcctcagg cgtatcagag
     1381 gaacaccatt cgttactcga atcagcttta aagagaatgg aagaaaaatc tttccaagta
     1441 gttactggaa agacgctttg gacgcaagat aaggaaaaat caggtccagc aagaaaacga
     1501 gcagatgaat tcaatcaaat gatgcaagat gaaaatatcc aaatgatctt tccgacttgg
     1561 ggaggagaat tattgattga gattctcgat tttgtcgatt ttgatgctct acaaccaaaa
     1621 tggatagtag gttattcaga tattagtacg ttgctacttg ctgtcacatt aaaaacaggg
     1681 atagccactg cacatggaac taaccttatt gatttaagag gagagtactt ggatgaaacg
     1741 acagcaatgt gggaaagtgt actgaaaaca agagaaggag aatcgattct acagcaatct
     1801 tctgaaaagt atcaaaaagt gtggccccat gagcattcat catcttctgt tttccatctc
     1861 actgaaccaa caaattggca atcagtcagc ggagatgcag taaggatgaa gggacgatta
     1921 ttaggtggtt gtattgatac gatcaggcat ttaatcggta ctccttatgg agatgtccaa
     1981 tcttttaaaa gaaagtataa tgatggaaag ccgatcttgt ggtttctaga aaactgtgag
     2041 ctatcgtcag ttgatttaag aagatcactt gttcagatga aactggcagg ctggtttgat
     2101 ggcagctcgg ggatcatgtt cggtagaagt gcggcagagg atttgttaga ttataaaatg
     2161 aaggatgtat acgaagatct gtcaaaggaa ttaaaactac cgatcttgta tgatattgat
     2221 tgtggtcatg taccgccaca actgacattg attaatggtg cctatgcaga agtagcggta
     2281 gaagatagaa aagcgactgt tttgcaaacg tttatttaaa aaattttttc ctgccttgga
     2341 gtctatcaaa gaccaataaa tctaaataga aaagaaagtg aaagaagctg acaaaatgct
     2401 gtcagcttct ttttgtataa catattagaa agtctaatta tctataactg tcacatcgat
     2461 taactgtttc acgtcattgg gatttcctgt aggaatagct actacagatg cattatcaat
     2521 gatttcttga gttgtttctc taatgaacgc tggataagat gtttgatctg caggcatttg
     2581 ccaatgtgaa ggaactggat aactagtgat tgtcccgtca tggttacttg aatacactaa
     2641 aactccctgg taactgtatt tcccagtgag cataaccaca tctttcggat agactgcact
     2701 ccctgcatca taaggggcga taggtgttcc tgcaggataa tgaataacat gtaattcgaa
     2761 atcacccgat gtatctctaa aagtattacc tctaatagct agccaaacac gtgcgtactc
     2821 aatttgtaga tcagaatatc ctgctaacgg atctattgac tgagaagact gtgaggaggt
     2881 tgtgtctatt gttgaagaag aaatatttgt ctcaactgta ctactagatg tagaagaagt
     2941 attgggctct gagactttac tgcttgttga agatgtattc gtttcagtag aagctgattt
     3001 ctgagtactt gatttttcag ctgaactggt ttgtctgatt gaacttttgt ctgtggtcgc
     3061 agatggatca ctattttctt tactaggaga cgtacatcct acaaggaata aagagaccaa
     3121 caa
//
LOCUS       DACCXW010000196         2967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCXW010000196 DACCXW010000000
VERSION     DACCXW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.204"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..696
                     /locus_tag="IVY71_002790"
     CDS             <1..696
                     /locus_tag="IVY71_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7986804.1"
                     /translation="EAVTTSLQVADSFEKKHKHVLEAIESIKRSVENSANVEDGSNFG
                     QMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEAFNEMEDVIRK
                     NTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMEVLGGKE
                     SRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQ
                     LNRQQRLFGDENE"
     gene            872..1213
                     /locus_tag="IVY71_002791"
     CDS             872..1213
                     /locus_tag="IVY71_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986805.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWGHVYLIHLWKQPRKPEYIYVIEDGLIDALEEYDRDNLIDISYYGQGKK
                     YIAEMEAEFDE"
     gene            1206..1952
                     /locus_tag="IVY71_002792"
     CDS             1206..1952
                     /locus_tag="IVY71_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7986806.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPQDVSMLEPASQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            1958..2644
                     /locus_tag="IVY71_002793"
     CDS             1958..2644
                     /locus_tag="IVY71_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986807.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2648..>2967
                     /locus_tag="IVY71_002794"
     CDS             2648..>2967
                     /locus_tag="IVY71_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986808.1"
                     /translation="MDHRSYYAIIPANVRYDDSLIPSAKLLYGEITALCNEKGYCWAS
                     NEYFANQYGVRKSTIQNWLKSLEEKGYIYREVKYKEGSREIEARYIRILGGGHPEKWV
                     GGHP"
BASE COUNT         1090 a          484 c          653 g          740 t
ORIGIN      
        1 gaagctgtaa ctacgagctt acaagtcgca gacagttttg aaaagaaaca caagcatgtg
       61 ctagaagcaa tcgaatcaat aaaaagatcg gtcgaaaatt cggccaatgt tgaagatggg
      121 tccaattttg gacagatgtt tgtggaaggg aacgagccag actcatacgg aagaagtcgg
      181 agagtttatt tcatgaatag agacggattt tccttgctag ctatgggatt cactggaagt
      241 aaagcaataa atttcaaact aaaatttatt gaagctttca acgaaatgga agatgttatt
      301 cggaagaata ctgttcctca aacaattgaa gacatgatga tctatcaatt agaagaaatg
      361 aaagatgtta aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa
      421 gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg aagttctagg gggaaaagaa
      481 agccgagctt atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa
      541 cgtacctttt caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt
      601 tcatttattg aaatgtggtt accagaaact gcgatccgca tggaaatcga tcaactgaac
      661 agacaacaaa gacttttcgg tgatgaaaat gaatagagct gaagcgctaa aaataggcgt
      721 aataattgct aatcgctggt ggagacacaa taaaccaaac atcctaagcc aacaacatat
      781 tgataagcaa aaagcatggc aacaaataaa aagcgactcc gccggcaagc attgagtcgc
      841 aaacaaaata catctaagga gatgttacca catgaaaaaa gaactttcca ctctagatca
      901 atatttgact gatcctagtt ggggcaaatc gaatatcaag gaaacaagca atcgaaaaat
      961 cagacgtaat cttttgacgg atgaagaact agcatgtgat caagacgatt tgggaaattt
     1021 tgtgagtatt tggggacatg tctaccttat ccatctatgg aagcagccaa gaaaacctga
     1081 atacatctat gtcatcgaag atggcttgat tgatgcacta gaagagtacg acagagataa
     1141 cttgattgat atctcttatt acggacaagg caaaaaatac atcgctgaaa tggaggcaga
     1201 atttgatgag tgaaaaggaa caacctttga aaaatagaag tgataatacg ctttttaata
     1261 ccttatacaa aataaatgtg aaagatgtta ctgaaaaacg aaataacctc acttatcttt
     1321 cgtgggcatg ggcttgggca gaagttagca aagtgtgtga ggcagtagat tatgaaatct
     1381 atcatgatcc agaaacatat ctgccgtatg tctttgataa gaaaacaggg tacatggttt
     1441 ttaccagtat cacagtcaac ggagtaaagc gtgacatgtg gttaccagtc atggatggtg
     1501 caaacaaggc aatgaaagat gagccatata cctacgaagt caatgattat cagtggaata
     1561 acgaaacgaa gaaaaaagag attgttggaa aaatcgaaaa gcgagttgaa gcagcaacta
     1621 tgtttgatat caacaaaacg atcatgcgct gtcttgtaaa aaatctagca atgtttgggc
     1681 tagggctata tatatttgct ggcgaagata tgccacaaga tgtctcgatg cttgaaccag
     1741 ctagccaaag aagcaaaaag ctattcttag atgctttaca actggttgct aacaagtacg
     1801 aaaaatcgat tgatgaagca attgttgcat tgactgatgc agcttccata accgccgatg
     1861 acagtaaatg gaccaagaga gacttgggca ttctaaaacg aggcgttaac tggattgaag
     1921 atcagtacag agaagaaaca aaagagaagt gatatgagtg tttaaaccat taatcgattc
     1981 atattcagcg gttctgaaaa agttcaaagg aaaagacata ggtgcaacga tcaatgaaga
     2041 agtgaacatt gatcgactaa agacgatgta tgacggctac gatggcgatc gagtcattga
     2101 aattcgtttt attgatccta gacgtttcac cgtacagcaa cgaaacttca tctatgcgct
     2161 gataggcgat atttttatcg atacaggcat gccaacggac ttctggaagg aattcttcta
     2221 cttccgtttt gaaggcgtca cagggcgcaa aataagcctc aaagacgaat cgaatacgac
     2281 tgtgagtgat gctaatgtct tagcaaatat cattttagat ttcatctttg aacaccatat
     2341 tcctttcaaa gaaggctatg agattttacc agcgaatcaa gagtattact tctacaaatg
     2401 catcacaaaa agagtctgct gcatctgtgg caaaacagga gctgatattg atcactttga
     2461 caaagcttta ggaagacgaa agcgcaaaga agttgatcat tcagagtaca catttgcagc
     2521 actctgcaga atccatcaca cagagaagca caagataggt gtgatcaatt tcaaaaataa
     2581 gtatcaaatc aaagggatca agttaaacca ggaaacaatc aagaaattaa gaatcggagg
     2641 atgaaaagtg gaccacagaa gttattacgc tatcatacct gcaaatgtta ggtatgacga
     2701 ttctttgata cctagtgcaa aacttcttta tggagaaatc acagctctat gtaatgagaa
     2761 aggttattgc tgggctagca atgagtactt tgccaatcag tacggtgtaa ggaaatcaac
     2821 gattcaaaat tggctgaaat ctcttgaaga aaaaggctat atctatcgag aagtgaagta
     2881 caaagagggt agtagagaaa tcgaggctag gtatatcaga attttgggtg ggggtcaccc
     2941 agaaaaatgg gtggggggtc acccaga
//
LOCUS       DACCXW010000197         2919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCXW010000197 DACCXW010000000
VERSION     DACCXW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.205"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1238)
                     /locus_tag="IVY71_002795"
     CDS             complement(<1..1238)
                     /locus_tag="IVY71_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7986809.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKM"
     gene            complement(1231..2823)
                     /locus_tag="IVY71_002796"
     CDS             complement(1231..2823)
                     /locus_tag="IVY71_002796"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7986810.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
BASE COUNT          814 a          593 c          466 g         1046 t
ORIGIN      
        1 aacatctttt gtaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tgtcgtcgag ttgtttgaag aaagcgccga ttttttgttg ttcctcttta
      121 ttcctagtaa aaggaaactt gatattagcg aaattctcat agaataagta ttctcgtacg
      181 cttccttcag tgttgcgctt gacctctttg ataaatgttg gtgacttgat aaactgaagt
      241 atgaactcat tatctaagtc ctcgctcatt ttcacaacta cataaagaga gctaacaatg
      301 accgtcatac caagattgtt aaatgcaatt gatcctacgt ttactcttgc tggattatag
      361 gcgaattcat ttggttcaac gaatttataa tttgtttttt ctaaatcatc caacctacta
      421 ccatcaaact gttctgtttg agaaattaaa cctgctttat tacttaccga ataggcaggg
      481 aagtctaaat ctttcgtgtt cttttttcca gtcttcttac taaactcttt gaacttacgc
      541 tgttcccaat cttccgtaaa tccaggaaac cgaatttctg gcacttttgc tccgttttta
      601 gggaacattt tttgtaagaa tccttttttc gtttccttta ataaatctag cttacgttgt
      661 tgaagagcga tagtgtcgtc cagttgtttg aagaagttcc caattttctt ttgttcttct
      721 aatattggaa attgtactat ccattttttc acttctgaca ttggtacgag atttgtaatt
      781 gctgaacctg gattagctcc tgtgagtttt ctttgcataa aatctgcacg aataaattga
      841 ataaaatatt ctgaatctgt gatttcttta tttattctaa gtctaatcaa agcctgattg
      901 aatacacccg gttttatttg ctttggtact cttgaaattc tcccaattgt tccagcgcca
      961 ctcatgataa aatcgcctgg ttctaattta aatttgatta attcattata ttttttttca
     1021 gtaattttat atcttgtttc gtaattatca taaatagcat tttgttgttc atagacaaca
     1081 aaattgctat tagatacaaa gaattctttc tttaaggcac tcccaaatgg tcccctcctt
     1141 actccatctt ttttatcaag taagcattca aacttacgct gttcccaagc ttccgtaaat
     1201 cctgggaaac gaatttctgg ttgtatatca ttactcatgt ttgaacacct ccagcgcacc
     1261 ttgtaaccac gcttcatttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc
     1321 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     1381 atcaattggt tcttcttctt caaacgtatc tacatagcgt ggaatattta agttatagtc
     1441 attttctttg atttcatcaa agtttgccaa atgagcgtat ttctcgacat cttttctttc
     1501 agcataggtt tcgaggattt tttgaatgtt ttcttccgat aatttattct gattttttcc
     1561 tttgacaaat tctcgactag cgtcaatgaa taatacgtct cgagtttggc ggttcttttt
     1621 caatacaatc accgtcgttg gaatcgatgt cccgaagaac aagttcgcag gcataccgat
     1681 cacagcataa atactgccgt cttctagtaa tttttgacga atgacccctt ctgcagcgcc
     1741 acggaataaa actccgtgtg gcaagacgat ggccatagtc cctgtttcct tcaaatgata
     1801 gaaaccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt ttccataacg
     1861 attaaaccga gaatcgtcta agaaggtcgt atctgctgac caattagccg aataaggtgg
     1921 gttcattact actgcatcaa atgtgtaagg ttcatctgtt ggccaatcct tattcaacgt
     1981 atccccattg cggagattca tttcttcggc atctacccca tgtaaaatca agttcatttt
     2041 tgctagattg tatgtcgttg tatttaattc ttgcccatga tatttaacgt tatctgggtg
     2101 agttaagtag ttacgcacat ttaacatcaa tgaacctgaa cccatggttg gatcaaaaac
     2161 actaaagaat cgtcgttctt tttgatccaa cgtcacgatt tgtgccatca tatcagaaac
     2221 catatgaggc gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     2281 cagaaattca taagcatccc cgatcacgtc cccatcgtga cctaaaactt ccacgtcatt
     2341 caatttctta atgacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     2401 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     2461 ttgcacaaag gctttgttta gatcattcaa ttggaacgtt gcttgttttg cttgatccgc
     2521 taagacgttg aaaagatact ctggcgaaat cgcatacccc aaaatatcaa cgattgttgc
     2581 aatcagatct tcttttgatt cttcatctga aagtaattcg cgatacaaca tcgtttgttt
     2641 gcttaccgta tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     2701 acggtcggat aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc
     2761 atccattttg ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
     2821 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
     2881 ctccctttta gaaaaagcaa aaaaaaaaag aaagaaact
//
LOCUS       DACCXW010000198         2908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCXW010000198 DACCXW010000000
VERSION     DACCXW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.206"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            70..531
                     /locus_tag="IVY71_002797"
     CDS             70..531
                     /locus_tag="IVY71_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP7986811.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            565..1200
                     /locus_tag="IVY71_002798"
     CDS             565..1200
                     /locus_tag="IVY71_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP7986812.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            1313..1948
                     /locus_tag="IVY71_002799"
     CDS             1313..1948
                     /locus_tag="IVY71_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986813.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            2247..>2908
                     /locus_tag="IVY71_002800"
     CDS             2247..>2908
                     /locus_tag="IVY71_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7986814.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FE"
BASE COUNT         1164 a          422 c          499 g          823 t
ORIGIN      
        1 aacacgggct ctttcctcat atatgagctt ctacaaaaaa aatcatatca cagaaatgag
       61 gaagaaaaaa tggaaaaaga gattttaata aaatctccag gaaaaacctt gattaaagta
      121 actgatgatt ctatttcaat tattagaaaa ggatttataa atttagttaa tcaaggtatt
      181 aaaggagaaa aaactatccc ctttaaaaat atatcagccg tacaattaaa aaaaccaggt
      241 atgagtaatg gttatataca atttaccttg ctaggcggaa acgaaagtcg cggaggtata
      301 ttagcggcca cgaaagatga aaacaccgtt atgtttacaa aaaaatattg gaatgaaatg
      361 gaaaatttaa aaaaatatat agagaagcaa caatctatat tagataataa ttccaaagaa
      421 acacaaattt caagtgcaga tgaaattaaa aagtataaag agttgttaga tgaagggatt
      481 attaatcaag aagaattcga ttttaagaaa aaagaattgc ttggtttata atattcaata
      541 tcaaaagaga aagaggatct attaatggac ggtatatttg gttttactgg tattgtttta
      601 tttattatag gtttaatcat gttaattgta agatttatca aaaaaacaaa taagaaaaca
      661 cctatgattc tccttattat cgggattatt tttactaccg taggtttctc gctttcctca
      721 tcaaacgagg caaatgacaa taacaaacag gaaagctcat catctgatac tcaaactact
      781 acatctaaca gcagcaataa aagcctaact caatttgaag aatttttaga gacaaataat
      841 catgactggg gtgctttctt agatagttat tattctatta ctccagctac tgagcaaaat
      901 acagctttta ctaaatatat tagtggtaaa acatatactt ttgaaggaac tgttatcgaa
      961 tcaatgacta ctagaatagc cattatagca gacaaagagt acgatgataa aagttggaat
     1021 gatatttctg caacacctaa agtttcatat gtaatttttg cgaaggactt aaacaatgct
     1081 gataccttta caaagggcga taaagtaact tttacaggag aaattagttc aagaggatcg
     1141 aacatagaaa aatcctatgc tcaatgggat atgattaata gtaaagtaag taaaaaataa
     1201 atgaaccacg ccccactaag agttagattg tgcacaataa taaaacctat acaaaagagt
     1261 gccatcagga gtcacaatgc ttgtaaagga aatgacaaaa taggaggtaa ctatggataa
     1321 tagcaatgaa aaactaacca aaaaagaata tcttgaatta atagttgaag gcggtattgg
     1381 agcaattcct tatattggtg gcgttgtgca gtcactttat tttgggtcta aaaacgagaa
     1441 gcgtttcaaa cgcatcgaga aattctacaa aaatttaaac gaatcattag aaaacattcg
     1501 cgatcaaatt ccagaagatg ttttcaactt agagaacaag gaccaactca ttggaatatt
     1561 cgaagggata aatgacgagg tagaaaaatc aaaggcacaa aataaaatag atatgtataa
     1621 aaatctttat aagaattgtt tacttcgaat taacaacgct tcatgggata acgaggagta
     1681 ttatcttctt gtactgaatc aattaacatc gattgagatt caattgttag catgcttaat
     1741 gagtcgtgga aacgaaaatt tcactggaaa tatcagcatg tctggctatt cacaagagct
     1801 aatagatggt agcttaaata gattatctga ttttggtctg ttagaaaaaa cgattagctc
     1861 catagtatta ggtgatgtcg gcaaacaaaa tatgggctac aaaatatctc atttaggaat
     1921 tcaatttatt aactcaactc ttacttaagt tttctttcta atatgacatc tagacctatc
     1981 agcaatgcaa caataaaaaa ccagacatac caataatcaa caaataatct aaactctgga
     2041 agttcagtta ttcttttaaa aatcacttta atcataataa acacctctga ttggatgatc
     2101 taatacacat ccaattatac cattaaaacg acaaatagca catccttgcg ccggcaagca
     2161 aaaaggatgt gcaaaaaata ataaaaccca aacaatgggc ttctttatag ttcctattgt
     2221 atcagagaaa gagagctgat tcaattatgg caagattagt caaacgtgga aatagttggc
     2281 aatacgaaat ttcatacaaa aaagatgacg gaaaatacac gaagataaga aaatcaggat
     2341 ttaagacaaa aggagaagca aaagatgccg ccaacgaatt agaatataac ttgaacaaag
     2401 gccttaaagg ggatcgcaaa aatctattat tatcagatta ctttgaggat tggatgcaac
     2461 tttataaaga aggaacagta tctcctatca cttatagaaa atacgaagat acgttaatga
     2521 acataaagaa atatatgcca gcggtattga tttctgattt agatagagtg ggatatcaac
     2581 gctttttaaa taagtatgct aaagaccatg taaaatctac cgttattaag tttaataacc
     2641 atattagagc gtcgttgaaa gatgccgtag aagaaggatt aattccgttt gatccaacta
     2701 gaaaagcagt aatcaaagga aaagattcat tgaagccaaa agaagataaa tatttagatt
     2761 atgatcaatt taaatcttta atgaaactcg tagaagaaaa cctttctgcg cagtactctt
     2821 ctcctatgct cgtgttagtt gctggtgcca ctggaatgcg atttgctgaa cttctaggat
     2881 taacatggga agatatcgat ttcgaaga
//
LOCUS       DACCXW010000199         2840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCXW010000199 DACCXW010000000
VERSION     DACCXW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.207"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1350
                     /locus_tag="IVY71_002801"
     CDS             <1..1350
                     /locus_tag="IVY71_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7986815.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="IVY71_002802"
     CDS             1484..2827
                     /locus_tag="IVY71_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7986816.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1114 a          389 c          550 g          787 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg
//
LOCUS       DACCXW010000200         2818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCXW010000200 DACCXW010000000
VERSION     DACCXW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.208"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(360..926)
                     /locus_tag="IVY71_002803"
     CDS             complement(360..926)
                     /locus_tag="IVY71_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7986817.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(926..1882)
                     /locus_tag="IVY71_002804"
     CDS             complement(926..1882)
                     /locus_tag="IVY71_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7986818.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1882..2538)
                     /locus_tag="IVY71_002805"
     CDS             complement(1882..2538)
                     /locus_tag="IVY71_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7986819.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          883 a          555 c          510 g          870 t
ORIGIN      
        1 aatttgactg atattaagtt agctattgat atatgaatga catacaaaaa tataaaatct
       61 tttaaaaatg cttatatatc aatgtttttg ccattctttt atatatctgt tatatgtcaa
      121 aaaaatgtgt tcaatttcaa tttgtatttg aataaagagg ttgtgaaaaa gctgtcctgc
      181 atcaagtaat aagaacagcc aatcaaaaac agcttctcat gtttttgatt tggttggact
      241 tattaccgca gatataagct tttgagcacc gtgaataaag aggttatgaa atcaacgctc
      301 taacctgagt attagagaaa aaaacgcaaa acatatcatt tatgttttgc gttttttatt
      361 tatctttttt tctcaataca atataaaata ggcggctggt tcttttggtt gataaaccga
      421 taattcagca cattatatgt ttcctgtggc agtttggaac aaaaagaatc aaccatatcc
      481 agttcttttt ctccaccttc atgaccatag tagacgacaa ggatcaatcg tccgcgaggc
      541 acaagtcgtt tcaatatttc ttccatagct gtgcgtgtag tctctggcat agtgatgatt
      601 gctttatcac tttttgggag ataccctaga ttgaaaatcc ctgctttgag attttgttgt
      661 tcagctatca cttctcctaa cgtttcgtgg ccttgtagaa acaatgagac acgttcttct
      721 aactgacgct gttctagcct ttcacgagta gcatcgattg cttgttgctg gatatcaaaa
      781 ctatagacat gaccagtttt tccgatgtgt tctgctagaa aaaccgtatc gtggccattg
      841 cccatcgttg catctacgac gtgatcccct ggttgcaaga tttcttgcag taatgtatgg
      901 ctgaaatgca aagcagtttg caacatcaga cgctcacctt tttccgtaca tcatattttc
      961 cttggaagct atctctgcga agaagttctt catcaattgc attcaagact tcccattttt
     1021 tcaagctcca cattggtcca atcaaggaat cccacggcgc atcccctgtc aaacgatgga
     1081 tgatgatctc ttttggtatc atttccagct ggtcgcaaat gacattgaca tagtcaggac
     1141 ggctcattaa ttgaaggcga ccttcatgat agtcccgcaa cattctcgtt ttgcgcatca
     1201 gatgcatcaa atggagcttg attccttgga tatctgaatc taaaatggtg cgtctgacat
     1261 tttctttcat catttctaaa ctttcgcctg gtaatccgtt gatcaaatgc gtacaaacac
     1321 ggatattatg tttgcgtaat ttagccacgc catctaaata agtttgataa tcatgtgctc
     1381 ggttgatcag ttttgaagtt tcttcaaaag ttgtttgcaa tccaagttct acccataaat
     1441 acatccgctc gttcagttca gcaaggtaat caactacttc atctggcaga caatccggtc
     1501 gtgtaccgac tgataatcct acgacacctg gtaaattgac tacttgctca aatcgttctt
     1561 tgattatttc aactggcgca tgggtgtttg taaaattttg gaaataaacg atgtattgtt
     1621 ggacatgagg ccatttttga tgcatcatgt cgatttcttt ctggaattgg attggaagag
     1681 ggtcttcagg tgcaacgatc atgtctcccg aaccagatac actgcaaaaa gtacaaccgc
     1741 catgggcaac agtcccatct cgattcggac agtcaaatcc tccatcgatc ggcaccttga
     1801 aaattttccc gccaaactca tttcgtaaag cgtaattcca agaatgatat cttttatttc
     1861 cttctgaata aggaaaagtc attaaaagtc cttctttctg taagagttat ctatttgttt
     1921 tcttttttct ctatacactg gataagtcag gcacagccaa gaaagactga gtaagtaacc
     1981 gccgatgata tcagtaggaa aatgaacgcc cagataaatg cggcttatcc cgacacttac
     2041 tataataaac atcatcagca attgaagcaa tgtgcgtaag gttctttttt gaacaaaagc
     2101 tggaagcaga aatagtaaag aaccgtaaag aatcatactg cctgctgcat gtccactagg
     2161 gaaactgtaa cttgtttcaa ctactaagtg cgccaatgaa ggacgctctc gcatgaaaaa
     2221 tagtttgatc accgggttga tgattccagc aattacaata aatccagaaa cgaaccaaaa
     2281 tgtttctgcc ctttttttct tataccacat ccataaagcc gcacaagcaa ataaaatgat
     2341 gacggtaaga ggattaccga atttagtcat aaagagttgg tagctgttca gatcaggata
     2401 agaagctctg acaatgcctg taaaccactg atcgaaacca gaaagccacg acagatggaa
     2461 ctttacgatc cagcccaaaa tgaaaaacag gacaagaaag aagctaccag ccacctgaaa
     2521 tgatgtttta tatttcatac aattcctcct ttgaagcaat gacttattat agcatttttg
     2581 gtccagcgaa aaaaacggta aagagataaa accaatgtat gattcctctt tgaataacca
     2641 atattcttat aaaaaaaata actgtaatcg gaaagacatt ttttggtaat gcaaatgaaa
     2701 tgttagggaa ataaaatcag tgtttttcga aatcctttta aacaagaacg tagacgtagg
     2761 aagaacttga aaatgaacag aagttgttca ttacggtaaa ttacaatatg attacaaa
//
LOCUS       DACCXW010000201         2753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCXW010000201 DACCXW010000000
VERSION     DACCXW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.210"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(16..1035)
                     /locus_tag="IVY71_002806"
     CDS             complement(16..1035)
                     /locus_tag="IVY71_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7986820.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(1046..1243)
                     /locus_tag="IVY71_002807"
     CDS             complement(1046..1243)
                     /locus_tag="IVY71_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7986821.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(1266..1559)
                     /locus_tag="IVY71_002808"
     CDS             complement(1266..1559)
                     /locus_tag="IVY71_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986822.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            complement(1596..1733)
                     /locus_tag="IVY71_002809"
     CDS             complement(1596..1733)
                     /locus_tag="IVY71_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP7986823.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            complement(1735..2181)
                     /locus_tag="IVY71_002810"
     CDS             complement(1735..2181)
                     /locus_tag="IVY71_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986824.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            complement(2184..>2753)
                     /locus_tag="IVY71_002811"
     CDS             complement(2184..>2753)
                     /locus_tag="IVY71_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986825.1"
                     /translation="DWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTE
                     KGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATF
                     IKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGMLTGPSGTSSVSKAMYVR
                     KDLGTIITNSEFAANEWFTFHGNYWVGEE"
BASE COUNT          745 a          578 c          448 g          982 t
ORIGIN      
        1 aacgaggggc tatttttatc gttgcggaat atttaaatac cagcgtttat catgaaaatc
       61 ttgtgctccg cctttagtgt tcccttctgg atcattcgtt gcccgcatca ttacatagac
      121 tttcttatta gggaagttgc gcatgttaaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacat ctggtctata tattccatca gcttttactc gagctaattc
      241 tttcccagta ttgtaatcca taatgaagat atactcgtat ttatagttag cgatgtgcca
      301 accagccaca tgtaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagggtag gatttgcagc acctgctcga gttggatcaa tgactggttt
      421 atcatctgaa gttgtcggat tttcatcggt aaatccatga gccaaatcat aggctaattt
      481 ttctttactt acgcccattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat ccctggttga ttatacggtg tgtctagtgt
      601 aagtggaata ccatatttca ttgctgaatc tctagctaat tcaacgtatg ccttgtagtt
      661 tttctcaaac gttgctttat catgtgtgtg ttgtaactca atctgcacag gactatttgc
      721 attagcatac gaaccagcac cgtactgtac ataaccaggt tgaccgactt gataaacaat
      781 tccgccgtct cccacaatat aagcagtata agcgctagtc catgaacgtt gcatatactg
      841 cgcttcattg cgtcctgttg ctgtttcatt agccgtttca tgcagtaaaa tgtactgatt
      901 atttgctact tgtgagctac cttcgtttgg gcctaaatta aattcattgt taatcgtata
      961 ggcaaaccca ttaattggca ataaaaaaag agccgttaat aggctcatcg cagtaatagt
     1021 aattttcttt ttcatttgtt tcctcctatt ttttcaaatt ataagccgac acgccagtga
     1081 taacgcctaa aaatgttgct actgcattga tagtgagtac tgtcatatct gttccattcc
     1141 atccatacgc tttgcctagt gttgcaacta atacagatgc agctggcaat actgttaaaa
     1201 ctgtccattt aatgacttga taatacttat cgggtaaaat catttcttct taactccttt
     1261 cttttttacc gagatctttc tctaaataaa gttttgattt gttgtgtgtg ttctaccaat
     1321 ttttctgtat gtgtatctaa tctttcatcg tgtttcttta gttcttcatg aatcatcaat
     1381 cgatctgatt tgctcgattc taaatcttta gtcagcaaat ctaaattgtg acttactttt
     1441 gaaagagttt cagtaatctt cgagaaagat gcagtgactg gtcttattac taataaaatc
     1501 aaagaaacaa tcgcagtgat tgatcctgcg attgttcccc attctcctaa attaatcatg
     1561 tgacaactcc tttaccttaa ataaaaacgc atcacttaag atgcgctctt atctttatta
     1621 atgatttttt ctgcttcttc gtctgtaatg catagtggaa cgaatagtcg aacttgatcg
     1681 tcagtaaaac agccccaatc atacatcatt ttcacatcgc taaaactaaa catactactc
     1741 acctcccttt gaagctggat ttagttgctc tttaatttct gcaatgtctt tgctattttg
     1801 gaacgaagca agcatcgttt ttgaattgat ttgtgctaaa ccatccgctt tttctttcaa
     1861 tgcagtattt tcctgtttaa tcgctacatc gcttagcatg agtttggcgt tgagctgttt
     1921 taaatcgccg ttctcatttt ctaacgactc atacatcgct ttgagattgt ttaaatcgtt
     1981 gtgatctagt acattcacta aaacaatcca ttggttcagt ttaggatcaa acatctgatc
     2041 agcaatcgtt agtggttccc catcagcacg aattccttcg agtggtggaa catcggtaaa
     2101 tggaacagtc ataaccatat catcaagcac ttgtccagca tactctccac ctgttcgtcc
     2161 gtatttccaa atatttttca tttttattcc tcccctaccc aataattacc atggaatgta
     2221 aaccattcat ttgcagcaaa ttcgctattc gtaataattg ttcctaagtc ttttcgcacg
     2281 tacatcgctt ttgatacact acttgttcct gacggacctg taagcatgcc atgaacaatt
     2341 tttgcgacaa attcatctgg gacttcatca tcaagtggta caccaaaagc ataagctttt
     2401 ttgaacttta ctgtaccatt tacaataact tcttttccgt gtttaataaa tgttgcatac
     2461 ccatcgataa acgattgact gttgtcttta gttaatgtat aaacagtgca atcattttca
     2521 ctgatcactt tatctcctgc tatttgaata ccatcggcaa agtttttcaa accttcaact
     2581 ctctgaggat catacacatt aacagcatca ttcaagcctt tttcagtata ttcaggtgtg
     2641 acatcccaac tgtaatcatt gggattgtta ctgtctttca agccttcacc aaagtattta
     2701 aactgactaa tattcggggt tcgtgtgtcg cctttttcga tcttgagcca gtc
//
LOCUS       DACCXW010000202         2753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCXW010000202 DACCXW010000000
VERSION     DACCXW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.211"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..200
                     /locus_tag="IVY71_002812"
     CDS             <1..200
                     /locus_tag="IVY71_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986826.1"
                     /translation="GKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAID
                     QEAYLKKKALERANRQSKAPF"
     gene            212..481
                     /locus_tag="IVY71_002813"
     CDS             212..481
                     /locus_tag="IVY71_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986827.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            644..943
                     /locus_tag="IVY71_002814"
     CDS             644..943
                     /locus_tag="IVY71_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986828.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            943..1245
                     /locus_tag="IVY71_002815"
     CDS             943..1245
                     /locus_tag="IVY71_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986829.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            1285..1650
                     /locus_tag="IVY71_002816"
     CDS             1285..1650
                     /locus_tag="IVY71_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986830.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            2277..2459
                     /locus_tag="IVY71_002817"
     CDS             2277..2459
                     /locus_tag="IVY71_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986831.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            2472..2666
                     /locus_tag="IVY71_002818"
     CDS             2472..2666
                     /locus_tag="IVY71_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986832.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT          992 a          429 c          619 g          713 t
ORIGIN      
        1 ctggtaagac ttataaaaac tatttggcaa ctattcgaag ttgggcaagg aaagaaaaaa
       61 gtgaacctaa gaacgcaagc agtggataca agcgcacagg aagacgagag aagcttcctg
      121 aatgggcaat cgaccaagaa gcctatctca agaaaaaagc gctagaacga gctaatagac
      181 aatcaaaagc accattctaa gaggtggaaa aatgaagatc gattatctag aactaattaa
      241 tgaaatagcg aattataaaa agggcgagga attagacgtc ctgagagacg tatatgatca
      301 actcgaagaa gctggaattg aaggaattaa gaatgatcgt tcgagttgga gtaaactcag
      361 atactatttc gcactctata tcgatggaac gcaattaaga aatttagcat ataccaaatt
      421 actattcatt gattgtgtta aaggcttgca aaaacatctt aatgaacttg agcaggtgta
      481 aacaagatgg acctaaagac atttacagca cagatcgaat taatgcatca agaagcttta
      541 agacaaagtg tgtcgtacga agacaagtgg ctcaacacat ttcatggcgg acgtgagagc
      601 gcacttgatc aagtactcaa attactgaaa ggagaatgtc gggatggata agaaagcggc
      661 aatgcagcga attatcgaat tgacttattc agaagattgg caggaagata aaaaaatagt
      721 tgcagaagtc caaaagctcg gtaaatcaat gtggactgaa aagcccaaac ggagaacgcc
      781 gagaaagatt gcaatctggc atggtgatcg aattctagta acgggtaccg ctaaacagtt
      841 atctgaaatt actggtctga gcaaaaatat tatttgggat agagctagga gcttatggat
      901 tgattcaaaa ggaagacagt ttaggtatgt ggaggagaaa taatggacga actaatcgcg
      961 aaagtagagc agtgggcaaa agataaggga ttggatcaag cggatttcag caagcaaatg
     1021 ttaaaaacgg tcgaagaaat cggggaagtt gccgcttctc tagctagaaa agatgaacat
     1081 ggtttaagag acggaattgg cgatgtagta gtaaccttga tcatcttggc aatgcaaaat
     1141 gatatggatt tatacgagtg cttggatttt gcttatgacg aaatcaaagg tcggactgga
     1201 aagatggtca atggtgtttt cgttaagtcc agtgacctga aataattcca ttaatggaag
     1261 aaataactga ataggaggat agacatgatt gatgcaaaac aagtgcaaaa acagaaagat
     1321 ggaatgttga tgtttgaagc atatgttcta ccatttctta atcaatttga agttttggaa
     1381 tgctctgcca gcggagaaga attggagtat gttgtaattc gtgaaaccaa agaaaacgtt
     1441 cagaagctaa acgaatttct ttgcactatt aactgttggg atatgatagc tccaggattt
     1501 ttatgtccag ctatgggaga atttttggaa tattgtcgtc ttgaagatgc aggcactttg
     1561 gatttagctt atctggtata caactaccta aacatcaaca cagatcattt gtggtttggc
     1621 actgccgaaa gaaaatgggt agtccgctaa ccaccaatat caatgaaaaa aggcggtttc
     1681 ctgaaagtgg aattgccgcc attgctgtaa ggttctttac gcgactcaag gtcacagaaa
     1741 tataaatgca aatctctttt gaagtgatcc aaagctctcg gaaaatggaa gttttttgtt
     1801 tcaaagaata aatgagattt gcgtcaagaa cctgagagtt atttccaaaa aagcttccaa
     1861 agaaccgaat gagctaagga catcatgtgt aaacacataa tatagctctc ctttcacggc
     1921 taatcataac atgaaaaata ttcgatgtca gtaggaaaaa aggtgaatta tgaaaagaat
     1981 attaaattat cccggcagta acagattctg caattaaact ccgaagctga tgtatcagta
     2041 tcaactgtag aatattatga gcgtggagaa agtcaagcag tcacaaatgt aaaaatcgat
     2101 aacgtcaacg gtcttatctg tttagatgaa ggcgatagta tgaatgaaat tgctgcagac
     2161 catgaattat cagaaactgc tttaattgat caaatcgaat tagctagaca atactttgtt
     2221 ccatatgcag atacctggtc caagcataag aatgacatca tttttccaga ggaacaatga
     2281 gagcaactga tccagtaatt atccttgagg aagccaaatt tatttggact cacgaagaga
     2341 tagagcaagc acgcttgctc ttttctcaag gagttaagcc gagcaaagta gctgaaataa
     2401 tgggtcaaaa gattcttgat gtcggattgc ttttgctcca tctagcagaa aaaatttgat
     2461 ttgaggtgaa aatgatgatt caacttgcag gcatccaaac agggaaaatt tatttttctg
     2521 gagaaagcaa aagcgaagcc aaccaatggc tattgaaaac ttacacgaac aataagaagc
     2581 taaggaaaaa atatcctgat ttgttgctaa aggatgatca gattatgccg gaaccgatga
     2641 ttttgacccg taaggaggga agttgaaact aaaaaaccag ccgtgtgggc tggagttgct
     2701 atttcttttt aataatgagt ttttctaaat ttaattcatt ctcacgagtt tgc
//
LOCUS       DACCXW010000203         2725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCXW010000203 DACCXW010000000
VERSION     DACCXW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.212"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(75..1874)
                     /locus_tag="IVY71_002819"
     CDS             complement(75..1874)
                     /locus_tag="IVY71_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7986833.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(1998..2594)
                     /locus_tag="IVY71_002820"
     CDS             complement(1998..2594)
                     /locus_tag="IVY71_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7986834.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          958 a          516 c          478 g          773 t
ORIGIN      
        1 aaaacgcata tacctgcggt ggcaggttat gcgttttttg ttgtataaag acttttttca
       61 ttaacgattt ttgattagcc gactcccaaa acaaaattta gcagtttatc tgaataagtg
      121 atttcttcat ctgtttgttt cacactttca tttagtagtc ccatctcatc ggttatgata
      181 ccatccacac cataaaacat catgcgactc atagcatctg aatcgttgat tgtccataca
      241 tatacttctt ttccgtcttg gtgggcagaa ttaatgaagt tacggttgat cgtactatat
      301 tccattgtca gaaaatcagc aggcgtcaca gggggcccaa caatattgaa aggtaagatg
      361 tagcccacat aaaagtttgg ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa
      421 ctttgcaaaa tatgatggtt ctctaagata actttgcggt atttatggac aaatcgttcg
      481 acaaggtcat ccgaatcttt tttagaggtc ttgatctcaa taagtaattt ttggtttagc
      541 ttatgtgctt tttccaaata agaatcaaaa gaacaaacag gggcttgcac tccgttttca
      601 gtaactgtaa gatttgtcaa ttctttcaat gtaagatcct gtggtgttcc atttacccct
      661 gttaagtgac gaagatttgc atcatgcatc actacgaaat gaccatcttt tgtttcctga
      721 acatccatct ctacataatc aggatgatag tctctgcttg ttttttcaag tgcttctaaa
      781 gtattttgcg ccccattttg ggtactcaca cctctgtgag agacagtcac tggttttgtc
      841 tgaacagctg aagtcagata atccatattg taaagacaga cgccaatacc gaaaaaaact
      901 gcaaaaagag tcaaaccagt gtttttcagc gcagagaaac gcaagttggg ggcttgtggg
      961 ataaaccact tagggatctc aggcaagaag ccttcgtcat ccatgaaatc aacaatgata
     1021 taaaaaatcc ctacggtaga catcacgata ttgaataata aaatcccttg taaaagcgtc
     1081 atagcaaaca cagcgctgat caatgcatag tctgggaaaa attgttcaac catactttgt
     1141 gccaaaataa caacgatata tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca
     1201 ataaattggc ccgtgatggc caaaagacgc gatttggtca atgaccagct ttcccgaatg
     1261 gctgctcgaa aaggacgatc tcgtagaatc atttccggta aagcaaaaat cagtcgaatc
     1321 ccaatatagc caaggaaaat ataaacgagt aaaaaggaac tgacgatgat ccaacgatta
     1381 gcaaaaataa aatccataat gaaagcgggg atttttattt ttgacagtag atcagaatta
     1441 aaacttaatc cgctgatcgg tagaatcaat aaaaaataag caaggaaaaa caaaaaagta
     1501 atgggtctca ctttcttcag ctgaagtatc gtcaagtgta agagttgctt caaagagatt
     1561 ggttctttct ttttaataaa aaaaacactc atcaaaagaa aagtaaattc gaaataaact
     1621 aacagtaaaa tcgtcatcaa aactacgatt aacgagagta gaaccccagg atgttgagaa
     1681 aaaattgaag gaatattgtc ataagacaaa tagtcaattg cccctcggtg taaaataaaa
     1741 cgggtcgtac tgctcaataa gggtaaacaa ataaataaaa taaagccatg catcagcaag
     1801 acatcacgga aataagcttg tgttcctttt aaaaagtccc aagcattacc taagctacgt
     1861 ttcaaataat tcatcctatc acctgctctt cacattgtag cgcaaatcta atgaaaaagg
     1921 aaagggtgca gtgtgttttg cagacccttt cccagattta acattgtcaa tcaatgacta
     1981 attttacaaa ataaatatta tccaagaaga ccacttagat attgacctcc ataaatcaag
     2041 gccatactat ataagaaaat cgacagtggt ttagcaatca aaatgattag tgtatatcgt
     2101 ttcactgaca tgtttgtcaa tccagccatc aaacataacg catcgtcagg agcaactgga
     2161 agaaaaatag ctagagcaaa cagcttctca aaacggtttt gattatccag ccagccaata
     2221 tatttgttat atgtcttatc gctaatcatg gaaagtatga atggctttcc ataacgacgc
     2281 cctagcagaa aaatgataat cgaaccaatg cagattccaa tatagttata gataaatcca
     2341 gcataagggc caaatatcag gacaccagct gcagtactga tccctccagg aatgattgga
     2401 ataactactt gcaggatttg gatgaagaca aaaataatcg gtcctaaaat gatggaatca
     2461 cctactaatc ctcttaacgc attcaaatct ttgaaaacac ctaaattaat aaaataaatc
     2521 gtcagcgcga ttgataggcc taatccgata atggatatga aatttatcag ttttcgagag
     2581 gcagcaatat tcattattca tcaactcctt agaggttctt tgttaactat actttactag
     2641 aaaagcaact aactgcgcca ttttttgata tgacagaaaa caaacaattt tgtaagttca
     2701 tcataagtat aaaagattga tctca
//
LOCUS       DACCXW010000204         2725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCXW010000204 DACCXW010000000
VERSION     DACCXW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.213"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..431
                     /locus_tag="IVY71_002821"
     CDS             <1..431
                     /locus_tag="IVY71_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAP7986835.1"
                     /translation="EYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIKD
                     RELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEYI
                     IKMIDSKKNLRYRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            403..1119
                     /locus_tag="IVY71_002822"
     CDS             403..1119
                     /locus_tag="IVY71_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7986836.1"
                     /translation="MKKEMYIMIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIF
                     PATFDEIYRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLEN
                     ADTLLTELRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPI
                     SYLLLLEKFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFY
                     QADKKIRSLWEIKNFLGVIS"
     gene            1116..2234
                     /locus_tag="IVY71_002823"
     CDS             1116..2234
                     /locus_tag="IVY71_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP7986837.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            2227..>2725
                     /locus_tag="IVY71_002824"
     CDS             2227..>2725
                     /locus_tag="IVY71_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7986838.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYV"
BASE COUNT         1030 a          342 c          541 g          812 t
ORIGIN      
        1 aagagtatat tttaggtgaa gaatatacta tagatgtttg cttaaacttt gattcgacac
       61 cagtaagtat tgttcctaga aaaagaatag caactagagg tggggaaata ctgaagggtc
      121 agattattaa agatagagaa ctaattgaga ctacgaaaaa gatgttggaa aaattaaatg
      181 caatcggaca tattactatt caatgtataa aaaacgaaaa gggaatattt tatttagaaa
      241 taaatgcaag atacggtgga ggagccccca ttagcattaa atcaggagct aattcccctg
      301 aatatattat aaagatgata gattcaaaaa aaaatttgag atacagagaa aattatttag
      361 ataaaatgat agcactgaga tatgatcaag cgatttattt agatgaaaaa ggaaatgtat
      421 ataatgatta aagcggtgat ctttgattta gatgacactc ttgtgtctga atatgattat
      481 gtaaaaagtg gatacaaatg tgtagctaga agattagcag tgcaaaaaat tttcccggct
      541 acttttgacg aaatatatag agtcctttta gatctttttg ctgaagatag taaaaatgta
      601 tttaatcgtc tatatgacta ttttgaaatt ccttatagta aaaaagaaat taaagaactg
      661 gtaaatgtat atcgagaaca caaaccagac ataaattttt tagaaaatgc agatacttta
      721 ttaacggaat taagaagtag aggatacaag ctggggatta ttacagatgg gtatgctata
      781 acccaacgaa acaaattgga tgctttgaaa gtagactatt tggtagatta tatttgtatt
      841 actgacgaat taggccgtga attgtggaaa ccacacccta tctcatatct tttattgtta
      901 gaaaagttta atttaaaggc acaagagtgt atatatattg gcgataatga gttgaaagat
      961 tttataactg ccaaatcaat aggaatgcaa acaattaaaa tagaaagagt aaatggtatt
     1021 tacagagaaa gcagtttgaa gttcaaaaga ttttaccaag ctgataaaaa aatacgttcg
     1081 ttatgggaga taaaaaattt tttgggagtg atttcatgaa aagtaaaaga attctattag
     1141 cttctccaca tatgagtgaa gaaggatatg aaaaagagta tgtaaaagaa gcatttgata
     1201 caaattgggt tgctccttta ggtgcgaatg tgaatggatt tgagaaagaa ttagcagaat
     1261 acgtgggagc tagtcatgct gccgcattat cttctggaac agcagctata catttagctt
     1321 tgaaagcggc tggggtcggt aaagatgaca tagtgatttg tcagtccttg acgttctcag
     1381 ctactgctaa tccaattctc tatcaaggag ctatacctgt atttgttgat agtgattacg
     1441 aaacttggaa tatgaaacct gaacatttgc gaacagcact agaaaaatat cctaacacaa
     1501 aggctgtatt agtagtacat ttgtacggtt tatcagctaa tttagacgaa attactaaaa
     1561 tatgcaaaga atacgatgtt actctaattg aagatgctgc agaatcatta ggaacaacat
     1621 ataaaggaaa atatactggt acatttggcg actatggaat ttattctttt aatggaaata
     1681 agataatcac aacttcaggt ggcggaatgc ttgtatccaa tgacaaagaa aaggtggaaa
     1741 aagtgagatt ttgggctacg caatctcgtg agaaggcacg ccattatcaa cacaatgaaa
     1801 ttggctataa ttatcgaatg agcaatatcg ttgctggtat cggtcgagga caattgaaag
     1861 tattaaatga aagaattgag aaaaaacgat atattcatag tttttataaa aaagaattgt
     1921 ctaaattgtc aaatatacaa ttcatgccag aaaataattg gaatgaaccg aattattggt
     1981 taagtgtcat aacaataaag ggaataaatg cgaatcatct tattgatgag ttagacaaaa
     2041 ataatataga agcccgccca gtatggaaac ctatgcattt acaacctgtt tttaaaaatt
     2101 atgattatat tggcgatgat gtagcagaaa ctttatttga cttaggagta tgtttaccat
     2161 cagatacaaa gatgacagat gaagatttaa aatatgtagt atctacgatt aaaagagtga
     2221 tcaatgatgc gtaaaaaaac tttctatcag ctttttggga aaagaatttt agatatccta
     2281 ttgtcaggaa ttgctctcat cgttttatcg ccgataatct taattgtagg ttttttagta
     2341 agaattaaac ttggttctcc aattatcttc aaacaagaac gaccaggaaa aagtgaaaaa
     2401 attttttcca tgtataaatt tcgtacaatg actgacgaaa gagaccacaa tggagaatat
     2461 ttacctgatg aaattcgact aactaaattt gggaaaatgc ttagagcaac aagtctggat
     2521 gagctacccg aactttggaa tattttgaaa ggtgatatgt cgatcgtagg acctcgtcca
     2581 ttattagtgg agtatttgcc actatatagt gagaagcaac ggaaaagaca caacgtaaga
     2641 ccaggattaa ctggactggc tcaggtcaat ggaagaaatg ctattagttg ggaagaaaag
     2701 tttgatttag atgtttatta tgttg
//
LOCUS       DACCXW010000205         2637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCXW010000205 DACCXW010000000
VERSION     DACCXW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.214"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            278..1063
                     /locus_tag="IVY71_002825"
     CDS             278..1063
                     /locus_tag="IVY71_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7986839.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1076..1927
                     /locus_tag="IVY71_002826"
     CDS             1076..1927
                     /locus_tag="IVY71_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7986840.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2051..2503)
                     /locus_tag="IVY71_002827"
     CDS             complement(2051..2503)
                     /locus_tag="IVY71_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7986841.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT          825 a          435 c          562 g          815 t
ORIGIN      
        1 aaggggctgt gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa
       61 gtagcttctt cggaaataag ccgaaatttc ttgaagctga ccacttctgt cacaacctct
      121 ttctttatag aaataaaagg acactagcaa ttcatgaaaa cgattgctag agcgtatcat
      181 ttgctttaga ctatatatct tggtgtacac tcactttaag gcggaaataa aagttatccg
      241 taataacagg tttaggtatg aaaaaggagt gggtactatg tacaaggaat tagaaaacaa
      301 agtcgcagta gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg
      361 gaaagaaaaa atgaatgtag ttgtcaacta taactcggat aaagaaggag cagaaaaagc
      421 agtcaaagca atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc
      481 tgaagaagga atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt
      541 ttgggttaat aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga
      601 ttgggaaaga gtgttaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt
      661 aaattacttt acagagaata ataaaaaagg aaatatcatc aatatgtctt ctgttcatga
      721 acagatccca tggccaacgt ttgctcatta cgctgcttca aaaggcgggg taaaattatt
      781 taccgaaacg gtagcaatgg agtatgcacc gaagggaatc agaatcaata gtataggccc
      841 tggtgctatc cgtacaccta ttaatgcggc aaaatttgat gatcctgaac aaaaagaact
      901 cttggaaagt atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc
      961 tgcatggttg gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg
     1021 tgggatgact ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg
     1081 tttattgatt gcattgatcc cggcagttgg ctgggggatc cagccactga ttttgaaaaa
     1141 gattggtggc agaccaacaa atgagatatt aggaactggt attggagcgg tgcttgtcgg
     1201 cttactcgtt caattattcg cttctccagc tggtatttca gtgactacat ttttgattag
     1261 tttactttct ggtgcatttt gggttattgg acaaacagga cagtatacat ctttcaactt
     1321 gattggtgtt tctaaaacga tgccaatttc gactgcatta cagttagtcg gaacctcgtt
     1381 gattggtgtc ttcgcttttg gagaatggtc tggaagtatg gggaaaatca ttggagcagc
     1441 agcaatcatt cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa
     1501 aaaaggcggt atcacaaaag gaattagtct attagcatca acaagtattg gttattgggt
     1561 atacagtgca ttgcctaaac tagttgatgc aagcggactt tccattttct ttccgcaaat
     1621 gatcggtgtc tttttagggg cagtgattta tgtgattttc cgtaaaccaa ccgcattttc
     1681 tgaagacaag agttggaaag caacgatcgt tggagccatc tttagtgtgt cagcattggc
     1741 ttatattttt tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa
     1801 cgtagttatc tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga
     1861 aaaaaaatta acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt
     1921 tatttgagga taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac
     1981 agtaagtact tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct
     2041 ttttaattgt ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc
     2101 attgactaaa gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt
     2161 ctttataaag atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt
     2221 actggactaa ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga
     2281 aacgcatcgt gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg
     2341 cttgatctcc gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa
     2401 cctttgttcc ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac
     2461 ctaattgttc ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt
     2521 gaatggttct actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt
     2581 taattaaggt cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaag
//
LOCUS       DACCXW010000206         2611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCXW010000206 DACCXW010000000
VERSION     DACCXW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.215"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            51..341
                     /locus_tag="IVY71_002828"
     CDS             51..341
                     /locus_tag="IVY71_002828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986842.1"
                     /translation="MKLTDATNYMSELSSNLETIDNLIKDLNSSYNMNDQVRVYLEKD
                     DVTLDYWAKSKSFNVSFEKEIIISALKDQRKVLYTELIKTKEFISELLEEKQ"
     gene            338..619
                     /locus_tag="IVY71_002829"
     CDS             338..619
                     /locus_tag="IVY71_002829"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986843.1"
                     /translation="MKTSYKQLDELISLKNRMKHNKTHLKYITECIDEVKKSSGDPYR
                     IKVSYDNHYLTLVSITKEQVINNLEKEKLKLTTAILEIQDEIIEILEEK"
     gene            619..891
                     /locus_tag="IVY71_002830"
     CDS             619..891
                     /locus_tag="IVY71_002830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986844.1"
                     /translation="MKYSLEQIDEIRSTILRIKLKRDELEGLLDEVNDISKDPEGIYL
                     LSFYYTKEERLFEISSARLIDYYNTKITNLKSEILSLEKRLQKLVK"
     gene            1013..1279
                     /locus_tag="IVY71_002831"
     CDS             1013..1279
                     /locus_tag="IVY71_002831"
                     /inference="COORDINATES: protein motif:HMM:NF037583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986845.1"
                     /translation="MARKLMQRNVTSTKVEFSEVIVQDGAPTIVPCEPVVLTGKLSEE
                     KALSAIKRKNPDKNVVVTNVSHETALYTMPVDKFIELADKSTQA"
     gene            1324..1695
                     /locus_tag="IVY71_002832"
     CDS             1324..1695
                     /locus_tag="IVY71_002832"
                     /inference="COORDINATES: protein motif:HMM:NF037548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986846.1"
                     /translation="MEIVKSTFDTQTPEGMLQVFNATNGASIPLRNAIGEVLELKDIL
                     VYSDEVSGFGGAEPSQAELVAFFTEDGKTYAGVSAVATKSAKNLIDMMTANPDIKPKI
                     SFVEGKSNGGQKFVNLQVVSL"
     gene            1780..>2611
                     /locus_tag="IVY71_002833"
     CDS             1780..>2611
                     /locus_tag="IVY71_002833"
                     /inference="COORDINATES: protein motif:HMM:NF017271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986847.1"
                     /translation="MAVRISKNDRAKLDKIYGKSNKARKKYNRLRQKGVEERQLPTVP
                     TSKKSLIDYVKSTNMSRSDFNKLLDELVDFAQPYNENYIFEINKRNVAISRAQIKEAH
                     IKTEQAQKAKEEHYKELNKVQVKKPTENTIVTPTILTELGADLPFQAIPDFNIDSFTS
                     PEGVQSYLENIGKQDEQYFDERDQLYYDNFRQAMFTIFNSDADDIVRLLDSMGLDLFM
                     KTYVSNFLDMNLDYIYDEAEVQQKKEQVYSKIAKVIESETGGEVPSYNPTKNITINSE
                     T"
BASE COUNT         1080 a          406 c          434 g          691 t
ORIGIN      
        1 tgtgattata tggtaaacta tatttgtaag gtaaaggagg ggtgaataaa atgaaactta
       61 cagatgctac aaattatatg tcagaactat catcaaactt agaaacaatt gataatttaa
      121 ttaaagatct taattcttca tacaatatga atgatcaagt tcgtgtttac ttggaaaaag
      181 atgatgtcac tctcgattat tgggcaaaat caaaatcttt taatgtttcg tttgaaaaag
      241 aaatcataat ttcagcttta aaagatcaaa gaaaagtatt atacacagaa ttaataaaga
      301 cgaaagaatt tatctcagaa ctattggagg aaaaacaatg aaaacaagtt ataaacaatt
      361 agatgaacta atttcattaa aaaatcgtat gaaacacaat aaaacacatc ttaaatacat
      421 taccgaatgt atagatgaag ttaaaaaatc atcgggcgat ccataccgta ttaaagtttc
      481 gtacgacaat cattatctta cacttgtttc tatcacaaaa gaacaagtaa taaataattt
      541 agaaaaagaa aagttaaaat taacaacagc gattttagaa atacaagatg aaattataga
      601 aattttggag gagaaataat gaaatactca ctagaacaaa tagatgagat tagatcaaca
      661 attctcagaa ttaaattaaa aagggatgaa ctggagggac tgttggacga agtaaacgat
      721 atttctaaag atccggaggg aatatatctt ttatcgtttt attacacaaa agaagaacgt
      781 ttgtttgaaa tttcgtcagc aagattaata gattattaca acacaaagat cacaaatcta
      841 aaatcggaaa tattatcgct cgaaaaaaga ttacaaaaac tagtaaaata attacacaaa
      901 aagctttaca aatataacac atcatgttat actaaaagag tagtaaggga acggaaaata
      961 ccttacttca cacctcaatc attcttatca aaatacaaag gaggaaaata gaatggcgcg
     1021 aaaactaatg caacgaaacg taacatcaac taaagtagaa ttttcagaag ttatcgtaca
     1081 agatggcgca cctacaattg taccatgtga accagttgta ctaacaggaa aactttcaga
     1141 agaaaaagct ttatcagcga tcaaacgtaa aaaccctgat aaaaatgtag ttgtaacaaa
     1201 tgtttcacat gaaacagcac tttacacaat gccagtcgat aaatttatcg agttagcaga
     1261 caaatcaaca caagcataat aaaacaaaaa caaaactaaa acaaaacaga ggagattata
     1321 atcatggaaa tcgtaaaaag cacatttgac acacaaacac cagaaggaat gttacaagta
     1381 ttcaatgcca caaacggagc ttcaattccg ttacgtaacg caatcggaga agttttagaa
     1441 ttgaaagaca ttctagttta ttcagacgaa gtttctggtt ttggtggcgc cgaaccatca
     1501 caagcagaac tagtcgcttt cttcacagaa gatggtaaaa catatgctgg ggtatcagcg
     1561 gtagcaacaa aatcagctaa aaatctgatt gatatgatga ctgctaaccc tgacatcaaa
     1621 ccaaaaattt cttttgtaga aggaaaatca aacggtggac aaaaatttgt aaatctacaa
     1681 gtggtttcac tgtagcataa aaatacagga atctagtaag ccacttagcg aagatcgcta
     1741 ggtggttttt attatgtttc tacattgagg tgtgtagaat tggcagttag aatttcaaag
     1801 aatgatagag ccaagttaga taaaatttat ggtaaatcta acaaagctcg taaaaagtac
     1861 aatcgtttaa gacaaaaagg agttgaggaa agacaacttc caactgttcc aacatcaaag
     1921 aaaagcctta ttgactacgt aaaatcaaca aatatgagtc gtagtgattt caacaagtta
     1981 ttagatgagt tggtagactt tgcacaacct tacaacgaga attacatttt tgagatcaac
     2041 aagcgaaatg ttgctatctc aagagcgcaa atcaaagaag cgcatattaa aacagaacaa
     2101 gctcaaaaag cgaaagaaga acattacaaa gagcttaaca aagttcaagt taagaaacct
     2161 acagaaaaca caattgtcac accaaccatt ttaacagagt taggtgccga cttacctttt
     2221 caagcaatac cagattttaa tattgattct tttacttctc cagaaggggt tcagtcttat
     2281 ttagaaaata taggaaaaca agacgaacaa tattttgacg agagggatca actttattac
     2341 gacaatttca ggcaagcgat gtttactatt ttcaattcag acgctgacga cattgttcgt
     2401 ttacttgatt caatggggct tgatctattt atgaaaacct atgttagtaa cttcttagac
     2461 atgaaccttg actacattta tgatgaagca gaagtacaac agaaaaaaga acaagtttac
     2521 agtaagattg caaaagtgat cgaatctgaa acaggtggag aagtcccctc atataacccc
     2581 acgaaaaaca tcacaattaa ttcagagaca g
//
LOCUS       DACCXW010000207         2585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCXW010000207 DACCXW010000000
VERSION     DACCXW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.216"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            11..1462
                     /locus_tag="IVY71_002834"
     CDS             11..1462
                     /locus_tag="IVY71_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7986848.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1811..2443
                     /locus_tag="IVY71_002835"
     CDS             1811..2443
                     /locus_tag="IVY71_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP7986849.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          964 a          376 c          508 g          737 t
ORIGIN      
        1 ggtaaatcaa atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact
       61 ccttatagca acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa
      121 agaaagaacc gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact
      181 tttgacaact tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc
      241 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
      301 tagtacgact tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc
      361 aagtaccgaa tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac
      421 aaaaccaaac catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa
      481 aactttttcg acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact
      541 atctggtaat gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa
      601 aattggtgag tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat
      661 tgcacaagcg atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta
      721 caatttattt gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga
      781 agatttgggt aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta
      841 tgaggaaagc tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga
      901 tttttatgca ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct
      961 tacaggacgg tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac
     1021 ctacgattta accaactatg ataaagaagt taaaggtcca caaatgagta agactggata
     1081 ccaagtgcct attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt
     1141 tcaccgagga cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg
     1201 taaggttgtt aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga
     1261 agatggatca accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa
     1321 tgttttgcaa ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca
     1381 ccttcatttg gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa
     1441 aaccttatta ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt
     1501 tggattctta gacctttgat caggatattg agactggaat tgatatctcg attacgatgg
     1561 gtttgacaaa taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc
     1621 tcatattttg agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt
     1681 acgtatcgtt cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa
     1741 atattttttt ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag
     1801 ggtaggtgca gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa
     1861 tggatataaa aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc
     1921 aaacttacta tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa
     1981 atataaaaat ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact
     2041 gcgaagtgaa ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt
     2101 atcggcaaag cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa
     2161 gcatcacatt gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa
     2221 ctgttcatgg aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag
     2281 ttatagtaac ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac
     2341 aaggacatgt gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa
     2401 tacaagtggt gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt
     2461 aaaaaaaaag aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag
     2521 aaaagatgct gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta
     2581 attat
//
LOCUS       DACCXW010000208         2510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCXW010000208 DACCXW010000000
VERSION     DACCXW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.217"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(55..420)
                     /locus_tag="IVY71_002836"
     CDS             complement(55..420)
                     /locus_tag="IVY71_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986850.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            628..1110
                     /locus_tag="IVY71_002837"
     CDS             628..1110
                     /locus_tag="IVY71_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986851.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1307..2197
                     /gene="pstS"
                     /locus_tag="IVY71_002838"
     CDS             1307..2197
                     /gene="pstS"
                     /locus_tag="IVY71_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7986852.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          848 a          417 c          502 g          743 t
ORIGIN      
        1 atatatgtat aaaaagaaga aagtagcttc tctttgtaca tatatggttt gtttttatgg
       61 atgccgagct tttttataag ataaggctac cgcccataac gacccaagga tttgtggaac
      121 taagtaaata cctgcaagaa gcaagatcag ctgggtcggt tttaaaggat gatagaagta
      181 acgccattca gtaaaattgg cgcaaaataa ataggaagtg acagtagata taatattggc
      241 aatgaataaa gatataatac gcctgttcct ttgaaaataa tatccagcaa aaagagataa
      301 aaatatcaat gtgatgaacc cgcttacgga atgataacga aaatcaagca gtaaggcgaa
      361 atgcacataa ggaacaaaag ataaaagcaa cggaaggttt ttccagcaag tttttatcat
      421 ctttcatcac cacctaacaa gtgttattat tttgttagtt ttattgtaaa ttgaatcctg
      481 caataatcaa ttactttttc attatttcta gattaaatga ataaactctc atgccattat
      541 ctctaaaaaa aaagtttttc cccctaattt ttttagatag aaaattgtat agtatgatga
      601 gagaaaaagt agtttggggg aacgttaatg agaagaaaag cagtaatcga tcggctttat
      661 ttattttttt ggttttttcc tatcgttttg ctacctttta catgggtaaa tgctgttgta
      721 caaggtgcgg agatattagg cctgatccaa ggaagcggat ttgtggtttc taggaatttt
      781 ccagctttga ttgttttcta tttagtgata agtgtaggtc tcttattacc aaaatctctg
      841 atcaatcact acaattggca gctgctttac ttttcttgct tattagtatt tacatctacg
      901 ttttctttgc tgcctcgctt gattatcggc cctaatggtt tgagcgagag tggattgaag
      961 gatttagctt attcctccat gtatttcttg acgatcaaac catggttcta tttagtaatt
     1021 gcctcattta ttcttgcaac atttttttac ttctggacag aaatcaggat aaaaagtgaa
     1081 cgatctgtat ctataaaaaa taactattga tggtggatca tcagtaaaaa aacatatgag
     1141 ctgcgtaaaa tgcatgtttc tcatatgctt tttttattat tatgtaaaga atgcgaaaaa
     1201 cgtttacatg atttttacat aacttaacta tatttttaca gcaaccatac gagggattta
     1261 cagtcgactg ataaacttta gttgtaaata agaaaggagc aataaaatga aaaaagtatt
     1321 gtttactcta ggtatgatgg gaattctttt agccggatgc ggctcaggat caggaaatac
     1381 agatggctct gccacacaaa ataattcaag tgactcaaat gaacaagtaa aaatcgttgc
     1441 ggtaggttcg acagcattac agccgttagt cgatgcagca caagaaagct ttgtacaaga
     1501 aaatccaaat tatcaaatct ctgtacaagg aggaggaagc ggaactggtt tgagccaagt
     1561 cgaagctgga gcagttacta tcggaaactc tgatgtattt gcagaagaaa gagacggagt
     1621 tgatgcttca aaattagtgg atcataaagt agcagtggtc gggatggcac caatcgtcaa
     1681 taaagataca gatgtcaaag atatcaccaa acaggaattg attgatatct ttacaggaaa
     1741 aatcactaac tggaaggaag ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc
     1801 aaacggcagc ggtacccgcg cgacattcga aaaatgggga ctggatggtg ctacccctgt
     1861 gcagtcccaa gaacaagatt catcaggaac agttcggcag ctggtaagtc aaacaccagg
     1921 agcaatcagc tatttagctt tctcatatct cgatgattcc acacaagcac taagtatcga
     1981 cggtgtagaa ccaaaagaag agaatgtagc agataacagc tggggaatct ggtcatatga
     2041 acatatgtac acgaacggaa aaccttcccc tgaagtccaa aaattcttag actacatgat
     2101 gacagaagaa atccaagaag gacctgtcaa agaattgggc tatttaccaa tcacaatgat
     2161 ggaagtagaa cgtgatcacg aaggaaatat caaataacta gcagataaac atgccacggt
     2221 agtcgttact attaaggaaa aatcaactat gtttccccac atatacattt ttcctttaat
     2281 tgtgaaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
     2341 tgaacaccgt ttattcgaaa aaaagagcta cgacatagaa ctgtcgtaac tttttttggc
     2401 tcttcgtcaa atcgtgttgg tgaagacaaa tttgaataaa acttagaccg atatgcggcg
     2461 cgttctgtgc gccgcatatt tttttagttt tcaaagatca gaccttgttg
//
LOCUS       DACCXW010000209         2471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCXW010000209 DACCXW010000000
VERSION     DACCXW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.218"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(94..1137)
                     /locus_tag="IVY71_002839"
     CDS             complement(94..1137)
                     /locus_tag="IVY71_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7986853.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1514..2467)
                     /locus_tag="IVY71_002840"
     CDS             complement(1514..2467)
                     /locus_tag="IVY71_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7986854.1"
                     /translation="MKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMILMIF
                     IFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVI
                     FQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFWISL
                     ICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALF
                     ALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLD
                     FLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT          885 a          461 c          474 g          651 t
ORIGIN      
        1 tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca
       61 tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag
      121 taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc
      181 ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc
      241 aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct
      301 gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt
      361 tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc
      421 aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa
      481 ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag
      541 tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag
      601 aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc
      661 tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca
      721 aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc
      781 aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt
      841 tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa
      901 ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa
      961 agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag
     1021 ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa
     1081 gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg
     1141 atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag
     1201 tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat
     1261 aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa aaatttgaga
     1321 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt acgacctctt
     1381 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta caaaaacatg
     1441 aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt ttcacaacct
     1501 attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg ctatgttctt
     1561 taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt gcaaacagac
     1621 attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg ttgaagtaat
     1681 gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag gctgcatagc
     1741 ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg aaatacaata
     1801 cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca aagtttttaa
     1861 gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc ggacgtgtcg
     1921 gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat aagaacgcat
     1981 agcagatcaa tgaaatccaa aagaaaattt ttccaataag tgggaagaag ttcccggcag
     2041 ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa attgcttcaa
     2101 ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg atcacttgga
     2161 agataacagc gcacagccaa atgaatttga caattggtgc aatactttca tatgaaacaa
     2221 aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag gcgatgattg
     2281 gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca aaaatgaata
     2341 tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg tgatagtctt
     2401 taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg atcggaataa
     2461 ttttcaaaaa g
//
LOCUS       DACCXW010000210         2444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCXW010000210 DACCXW010000000
VERSION     DACCXW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.219"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            288..695
                     /locus_tag="IVY71_002841"
     CDS             288..695
                     /locus_tag="IVY71_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986855.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="IVY71_002842"
     CDS             709..1110
                     /locus_tag="IVY71_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986856.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="IVY71_002843"
     CDS             1112..1483
                     /locus_tag="IVY71_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986857.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1818)
                     /locus_tag="IVY71_002844"
     CDS             complement(1519..1818)
                     /locus_tag="IVY71_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986858.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1824..2054)
                     /locus_tag="IVY71_002845"
     CDS             complement(1824..2054)
                     /locus_tag="IVY71_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986859.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          875 a          339 c          414 g          816 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt
     1741 ccatacttta taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg
     1801 tgtctcatta aaaagcataa ccattactcc gatctgtttt taaaattagt ttctacctct
     1861 aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca
     1921 ggttgtttat tttcggaaaa agctaatata gctaagattg aaacatccat ctggaattta
     1981 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
     2041 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
     2101 agcgatattt gaggggcaaa aaaggggcaa aaaactctta ctagtccgtt ctagcctaaa
     2161 aaattcaaac attgccaacg ttgcttttcc ttgatttgat gggatttttg tccagtgtgg
     2221 acaagtgaaa aacatttgtc ttgatgggcg gtatgatgta agaaaaagca acttgcttat
     2281 ctacaactga tcattagtga aagacagata aaaagaggct ggaacagaag cgtttaactc
     2341 caagaaataa gaagaaattc acggaaattg cttttcaaat ttttgtgaat ttcagcttat
     2401 ttccgaagga gttgcttctg cttccgctgt ttatacgtgt ttag
//
LOCUS       DACCXW010000211         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCXW010000211 DACCXW010000000
VERSION     DACCXW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.220"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            207..2069
                     /locus_tag="IVY71_002846"
     CDS             207..2069
                     /locus_tag="IVY71_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7986860.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IVY71_002847"
     CDS             2069..>2398
                     /locus_tag="IVY71_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7986861.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACCXW010000212         2384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCXW010000212 DACCXW010000000
VERSION     DACCXW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.221"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            81..2234
                     /locus_tag="IVY71_002848"
     CDS             81..2234
                     /locus_tag="IVY71_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7986862.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          813 a          423 c          530 g          618 t
ORIGIN      
        1 gctccgccca ttttttcttt tttattaaga agtttttggg tacaatgaag catgaatgta
       61 aataggcgga aggataggaa atgatgaaag attttatgaa aaaaatatta aatacttctt
      121 ggttagaaaa aataagaaaa ggtgcacaag aacctaagaa caaaacgttt cgccggtccc
      181 atgttccttt tcgcttaaat tttctttttt tcattatatt tacgttattt gttgcgttga
      241 ttgctagatt gggttatttg cagatcgtga acggcgaaga gatggaggcg cgagtgaagt
      301 ctacttctac gattacggtc caagaaagta ctcctcgcgg aatgatctac gactccacag
      361 gaaaagcttt agttacgaat aaagcgaatc aggccatcac atatacgaga ggaacacaag
      421 ttacggctaa agatttgctt gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg
      481 tagatgaaaa tctgacagat agagataaaa aagatttttg gctggctgat cccaaacatt
      541 tgaaagaagc cacaaaaaga ctttcggcaa aagaaaagca attagggaac agcgaacaat
      601 atagtgcgat ggtcaataaa gtcacagctg atgagattaa tttaaacgag gagcaattaa
      661 aaattgctac catctttaaa cggatgaata gtgcatatga attaaacact gtctatatca
      721 aaaactctgg tgttactgat caagagttag ctgttgtggc agaacgcgct tctgaacttc
      781 caggagtttc gactggcaca gactggacac gagaatataa tgcagcaagt tcactgaaaa
      841 gtattcttgg cagtgtgacg actgaaaagc aaggacttcc agcagatgaa gcagagaaat
      901 acctagcaaa aggttattct cggaatgacc gtgtaggaca aagttatttg gaaaaacaat
      961 atgaagatgt cctgcaagga accaaaaccc aatatgaagt ttcccttgat aatgaaggaa
     1021 atgtttcgaa tcaaaaagaa atcttctctg gggaaaaagg ttctaattta atgttatcta
     1081 tgaatgcaga attccaatca aaagtagaag aaattttgaa aaggaattat caaacattga
     1141 tcaacaacgg caaagcacag tattcgcctg gtgcttatgc agtggctatg aatcctcaga
     1201 caggagaagt gctagcaatg actggtttca gtcatgaaca aggttccaaa gaaatcaccg
     1261 agaatgcttt aggcacgatt actagcgcct ttgatccagg gtctgtagtc aaggctggta
     1321 ctttgacagc aggctgggca agtaacgcga tcagcggaaa tcaagttttg attgatgagc
     1381 cgattcgtct gcaaggtgct tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg
     1441 ctcttgatgc agtcaaagca tcggaattgt cttctaatac gtatatgatc aaagtggcgt
     1501 taaagatgtt aggactagac tatacacctg gaatgggact acctagttta gatgaagaag
     1561 ctaaagctta tcagcaattg agagactctt tcaaagagtt tggtcttggt acaacgactg
     1621 ggatcgattt gccgaatgaa tctcctggga tctcacgttc tgttgattat atgaagaagt
     1681 tcaatgcgga taatggcaaa gaatggtatg ctccaggtaa ctttaccgat ttggcatttg
     1741 gacagtttga cacatataca ccaatccaat tagctcaata tgcttcaaca gtcgctaatg
     1801 gcgggaaacg agtacagccg cacctagtca aagcgatcta tggcaatgac gaaaatggaa
     1861 acttgggtga agtgaagaaa gaaattggca caacagtaga aaatacggtc aatatttctg
     1921 cagaaaatat ggcgatcctc cgtgaaggat tccaccaagt tgtccatggt accgatcctt
     1981 acacaacagc caaaccatta gccagtgcga aaatggacct ttccgcaaaa acagggactg
     2041 cagaaacggt tgcggaaggt catccagata ttgcgacagt caatagtaat atcgtggcat
     2101 acggaccaac agacaatccg gaaattgcca tcagtgttgt cttgccgaac ttattagacg
     2161 aacaagacca cataaacttg acgattgcta aagaaataat ggacgcgtat tatgatatgt
     2221 tcatggcaaa ataaaaaaga ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa
     2281 ttttgtgaaa atagcttctc acatttttgc ttaaatttga cttcataccg agaaactggc
     2341 ttttgaacac cgtttactcg gcaaaaaagg ttgtgacaaa aatt
//
LOCUS       DACCXW010000213         2357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCXW010000213 DACCXW010000000
VERSION     DACCXW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.222"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..77
                     /locus_tag="IVY71_002849"
     CDS             <1..77
                     /locus_tag="IVY71_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7986863.1"
                     /translation="VVGICAIYCLKFFPMITRKVDERY"
     gene            216..701
                     /locus_tag="IVY71_002850"
     CDS             216..701
                     /locus_tag="IVY71_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986864.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            1157..1570
                     /locus_tag="IVY71_002851"
     CDS             1157..1570
                     /locus_tag="IVY71_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7986865.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            1707..1967
                     /locus_tag="IVY71_002852"
     CDS             1707..1967
                     /locus_tag="IVY71_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986866.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            2085..>2357
                     /locus_tag="IVY71_002853"
     CDS             2085..>2357
                     /locus_tag="IVY71_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986867.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLF"
BASE COUNT          732 a          372 c          390 g          863 t
ORIGIN      
        1 tcgtcgtagg tatttgtgcg atctactgct tgaaattttt cccaatgatt actagaaaag
       61 ttgatgaaag atattagaaa gctataatta ttaaaaaagt tattatcttc atgaaccttc
      121 cctttattgg gaaggttttt tcattttaaa ccatctgctt ttctttgttt aagtgatata
      181 ctgaataaag ataatctatt ttaggaggag ctatcatgca taaagggatt tacgcaggct
      241 tgttgggaac tgtaatactt ttttctgcct gtggatataa aacaaagaat tctgttaaag
      301 aatcttcagc agccatttca acaactgagg ttggttcaac taaaagtact tctactactt
      361 ccattactaa tacttctact ggtaatgtag attcgcaaca tagccaatca agtattcagt
      421 cgtttagcat ttcttcaaca tctgaaatgc aagaaagcac tgtaccttct cttccattag
      481 aaaactctga caaagcactt ctatttctta ttgcccaccg ttccgaatta caaagtgacg
      541 atattgttat tagcttttat caaaaaattg atcgagatta tttgttcaca gcttccagca
      601 aacaaatccg ttcccaagga ggatctggaa gcgttggttt ttatcgagtg tcacccgaag
      661 gatcgattac gatgacagat gcaaatggaa caccattttg attagatgat attatacgga
      721 tttacagtac tagcaagtca ttattggtat cattttttct gagaaagtta tacggagtat
      781 aaataagcat ttgcattaac ctaataattc gttacagcta tttctcttat gaatatactc
      841 acttctctac ttgctctctt gataaatttt tggtatctac ttcattttcg gtcaactgag
      901 ttaatgaaat ttattgtcgg gcggttttct agattcaatt tttctatatt tggatagaag
      961 aatttacttt tttacctttt gacatatttt cttcttatta ttttaatgat ccctttttca
     1021 aattagaaag atagcctttg tatcgtatta gatgaaatat tcacaaagct aaccattata
     1081 cttgcatttt tttcaaaata gagttattac gtaaataggg ataaataaaa aatacttatt
     1141 gaaaggatga tcaaaaatgg caaatgtacc tagcattcgc gacatgttcc ctgatttcaa
     1201 tgacgtgttt agtccagcat tcaatgattt tttaggtgtt tcaagttatc caaaagtaga
     1261 tttagtcgaa aatgaaaaag aatacaaact aactgctgat atgcctggct gtgacaaaga
     1321 agacacgaca gttgaatatt cagataatac tttgacgatc tccgccaatc atgagtccca
     1381 tacagaagat aaagaagatg gcaactatgt acggaaagaa agacattcgg tctcttacaa
     1441 acgttcattc tatcttccta atgttgatga agaaaaaatc actggaacct ttaaaaacgg
     1501 tgtgctgaaa ttggtattac ctaaaacagc ttatcagcca aaagaaacaa aaaaaattga
     1561 gttaaattag aacttatcgc ctctcgatga atgaggggcg atttttatat atttggataa
     1621 gaacgagtat gtttaaattt tcaaaatgag caaaaaaata ttttgttttt acgataaaga
     1681 tgttatgatc tctcttggta aaaaatatgg gagttgatca tttgattgtc ttaagtaaac
     1741 ttttgttttg gataccattt atcataatga ccagtgcttt cttttactta ttcaaaaggc
     1801 ctaacaaata cttactctta tctgcttgtc cgatgatcta ttttatccat aagattagag
     1861 gattttatta ttacgactct ggtatctcat tgttttgcta cgaaatcggc ttattagctt
     1921 cggccatctt tttcatattt gtgttagaat atttcagaaa aaaataaact tccagtcagt
     1981 tttggctgga agttttttat ttggtgcaga tcttttcagc tgttattcat acaagttgat
     2041 ccatttttct attattaagt acataccatt gttactcttt gaatgtgggg gagaaaaaaa
     2101 taaaaggagc tggtattttg ttagttatga gtaaagcttt gtttttactt ccctgcacgt
     2161 tcatgtttat tgttttatgt tatttcaaaa gaagaaaaaa atggtgctgg atcgatgttc
     2221 tactattcct tccctttttc ccgcttattt attttcttga agaaatatgg gatgatatgg
     2281 catgtgattc tgcttttctt tttagtcatt cattggggct tatgatctct cttacgatct
     2341 ttgccattgt gctattt
//
LOCUS       DACCXW010000214         2356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCXW010000214 DACCXW010000000
VERSION     DACCXW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.223"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(120..191)
                     /locus_tag="IVY71_002854"
     tRNA            complement(120..191)
                     /locus_tag="IVY71_002854"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Glu,seq:ttc)
     gene            complement(203..276)
                     /locus_tag="IVY71_002855"
     tRNA            complement(203..276)
                     /locus_tag="IVY71_002855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(240..242),aa:Ile,seq:gat)
     gene            complement(289..359)
                     /locus_tag="IVY71_002856"
     tRNA            complement(289..359)
                     /locus_tag="IVY71_002856"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Gly,seq:tcc)
     gene            complement(364..436)
                     /locus_tag="IVY71_002857"
     tRNA            complement(364..436)
                     /locus_tag="IVY71_002857"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(401..403),aa:Phe,seq:gaa)
     gene            complement(440..512)
                     /locus_tag="IVY71_002858"
     tRNA            complement(440..512)
                     /locus_tag="IVY71_002858"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Asp,seq:gtc)
     gene            complement(531..604)
                     /locus_tag="IVY71_002859"
     tRNA            complement(531..604)
                     /locus_tag="IVY71_002859"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(568..570),aa:Met,seq:cat)
     gene            complement(617..706)
                     /locus_tag="IVY71_002860"
     tRNA            complement(617..706)
                     /locus_tag="IVY71_002860"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(668..670),aa:Ser,seq:tga)
     gene            complement(729..800)
                     /locus_tag="IVY71_002861"
     tRNA            complement(729..800)
                     /locus_tag="IVY71_002861"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(765..767),aa:Glu,seq:ttc)
     gene            complement(822..909)
                     /locus_tag="IVY71_002862"
     tRNA            complement(822..909)
                     /locus_tag="IVY71_002862"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(873..875),aa:Ser,seq:gct)
     gene            complement(934..1007)
                     /locus_tag="IVY71_002863"
     tRNA            complement(934..1007)
                     /locus_tag="IVY71_002863"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(971..973),aa:Ile,seq:gat)
     gene            complement(1021..1091)
                     /locus_tag="IVY71_002864"
     tRNA            complement(1021..1091)
                     /locus_tag="IVY71_002864"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1057..1059),aa:Gly,seq:tcc)
     gene            complement(1093..1168)
                     /locus_tag="IVY71_002865"
     tRNA            complement(1093..1168)
                     /locus_tag="IVY71_002865"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1133..1135),aa:Asp,seq:gtc)
     gene            complement(1187..1260)
                     /locus_tag="IVY71_002866"
     tRNA            complement(1187..1260)
                     /locus_tag="IVY71_002866"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1224..1226),aa:Met,seq:cat)
     gene            complement(1273..1362)
                     /locus_tag="IVY71_002867"
     tRNA            complement(1273..1362)
                     /locus_tag="IVY71_002867"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1324..1326),aa:Ser,seq:tga)
     gene            complement(1386..1459)
                     /locus_tag="IVY71_002868"
     tRNA            complement(1386..1459)
                     /locus_tag="IVY71_002868"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Ile,seq:cat)
     gene            complement(1473..1546)
                     /locus_tag="IVY71_002869"
     tRNA            complement(1473..1546)
                     /locus_tag="IVY71_002869"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1510..1512),aa:Met,seq:cat)
     gene            complement(1574..1646)
                     /locus_tag="IVY71_002870"
     tRNA            complement(1574..1646)
                     /locus_tag="IVY71_002870"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1611..1613),aa:Ala,seq:tgc)
     gene            complement(1666..1739)
                     /locus_tag="IVY71_002871"
     tRNA            complement(1666..1739)
                     /locus_tag="IVY71_002871"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1703..1705),aa:Pro,seq:tgg)
     gene            complement(1749..1822)
                     /locus_tag="IVY71_002872"
     tRNA            complement(1749..1822)
                     /locus_tag="IVY71_002872"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1786..1788),aa:Arg,seq:acg)
     gene            complement(1833..1918)
                     /locus_tag="IVY71_002873"
     tRNA            complement(1833..1918)
                     /locus_tag="IVY71_002873"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1882..1884),aa:Leu,seq:taa)
     gene            complement(1935..2006)
                     /locus_tag="IVY71_002874"
     tRNA            complement(1935..2006)
                     /locus_tag="IVY71_002874"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1972..1974),aa:Gly,seq:gcc)
     gene            complement(2018..2090)
                     /locus_tag="IVY71_002875"
     tRNA            complement(2018..2090)
                     /locus_tag="IVY71_002875"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2055..2057),aa:Thr,seq:tgt)
     gene            complement(2108..2189)
                     /locus_tag="IVY71_002876"
     tRNA            complement(2108..2189)
                     /locus_tag="IVY71_002876"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2153..2155),aa:Leu,seq:tag)
     gene            complement(2197..2269)
                     /locus_tag="IVY71_002877"
     tRNA            complement(2197..2269)
                     /locus_tag="IVY71_002877"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2234..2236),aa:Lys,seq:ttt)
     gene            complement(2273..2345)
                     /locus_tag="IVY71_002878"
     tRNA            complement(2273..2345)
                     /locus_tag="IVY71_002878"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2310..2312),aa:Val,seq:tac)
BASE COUNT          647 a          646 c          546 g          517 t
ORIGIN      
        1 ttttacatta gaactgtcca aaaaagctta gcacaatcat ccataaagat aactctgcta
       61 agctttttaa aatcattctt tcagataaaa agaaaaagat actcaaaaag tatcttccat
      121 gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg
      181 accaacgggc caataaaata aatgggccta aatggactcg aaccatcgac ctcacgctta
      241 tcaggcgtgc gctctaacca gctgagctat aggcccataa aaactaaaag cgggtgacga
      301 gaatcgaact cgcgacaaca gcttggaagg ctgtggtttt accactaaac tacacccgca
      361 taatggcgcg ggacagaatc gaactgccga cacatggagc ttcaatccat tgctctacca
      421 actgagctac cgagccaaac ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca
      481 ggcaggcatg ttaaccccta caccacggaa ccaatgtttt tagtttttaa ttgcgggagc
      541 aggatttgaa cctacgacct tcgggttatg agcccgacga gctaccagac tgctccatcc
      601 cgcgataata tgaaaaagga ggataaggga ttcgaaccct tgcacggttt tacccgcctg
      661 acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccatgt aaaactatat
      721 taaattaatg acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt
      781 aaccgcttga ccaacgggcc aggtaaaaca aaatattgaa acggagaagg agggatttga
      841 accctcgcgc cggtttcccg acctacaccc ttagcagggg cgcctcttca gccacttgag
      901 tacttcccca aaaccaataa gattttgtac taatgggcct aaatggactc gaaccatcga
      961 cctcacgctt atcaggcgtg cgctctaacc agctgagcta taggcccata aaaaactaaa
     1021 agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtggtt ttaccactaa
     1081 actacacccg catggcggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca
     1141 ggcatgttaa cccctacacc acggaaccag tgtttttagt ttttaattgc gggagcagga
     1201 tttgaaccta cgaccttcgg gttatgagcc cgacgagcta ccagactgct ccatcccgcg
     1261 ataatatgaa aaaggaggat aagggattcg aacccttgca cggttttacc cgcctgacgg
     1321 ttttcaagac cgttcccttc agccggactt gggtaatcct ccatatgaaa cttacaacgc
     1381 cacaatggac cttgtaggac tcgaacctac gaccggacgg ttatgagccg tctgctctaa
     1441 ccaactgagc taaaggtcca ggctcttaaa aatcgcggcg gaggggatcg aacccccgac
     1501 ctcccgggta tgaaccggac gctctagcca gctgagctac accgcgaaaa agatattcat
     1561 tatttaaatg aaatggagcc tagcgggatc gaaccgctga cctcctgcgt gcaaagcagg
     1621 cgctctccca gctgagctaa ggccccgtgt ttaaatttat aattatcggg aagacaggat
     1681 tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc tacttcccgt
     1741 ttaattaacg cgcccaagag gagtcgaacc cctaaccttt tgatccgtag tcaaacactc
     1801 tatccagttg agctatgggc gcatatttga aatgccgagg accggaatcg aaccggtacg
     1861 gtgatcactc accgcaggat tttaagtcct gtgcgtctgc cagttccgcc accccggcgc
     1921 gattgctttg gcaaagcgga aaacggggtt cgaacccgcg acccccacct tggcaaggtg
     1981 gtgctctacc actgagctat ttccgcgtgt ttaaagatgc cggctaaagg acttgaaccc
     2041 tcgaccctct gattacaaat cagatgctct accaactgag ctaagccggc atgtaaaact
     2101 taaattatgc gggtgaaggg acttgaaccc ccacgccgta aggcgctaga tcctaaatct
     2161 agtgcgtctg ccaattccgc cacacccgca aaaatatgag ccgtacaggg ctcgaacctg
     2221 tgaccctctg attaaaagtc agatgctcta ccaactgagc taacggctca tatggaggtt
     2281 aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag ctgagctaaa
     2341 cctccatgga taactt
//
LOCUS       DACCXW010000215         2323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCXW010000215 DACCXW010000000
VERSION     DACCXW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.224"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            94..483
                     /locus_tag="IVY71_002879"
     CDS             94..483
                     /locus_tag="IVY71_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986868.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            487..1251
                     /locus_tag="IVY71_002880"
     CDS             487..1251
                     /locus_tag="IVY71_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7986869.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            1380..>2323
                     /locus_tag="IVY71_002881"
     CDS             1380..>2323
                     /locus_tag="IVY71_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7986870.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETT"
BASE COUNT          743 a          366 c          512 g          702 t
ORIGIN      
        1 aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat
       61 aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt
      121 gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag
      181 ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc
      241 gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg
      301 aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg
      361 tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc
      421 cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag
      481 taacaattga atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt
      541 gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag
      601 acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg
      661 atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc
      721 tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat
      781 ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga
      841 atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg
      901 ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat
      961 tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca
     1021 ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa
     1081 aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga
     1141 aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat acaactaatg
     1201 gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg
     1261 aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg
     1321 ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca
     1381 tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag
     1441 tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg
     1501 ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga
     1561 tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc
     1621 tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg
     1681 atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact
     1741 tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat
     1801 atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag
     1861 aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt
     1921 caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc
     1981 ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta
     2041 cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga
     2101 ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat
     2161 ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg
     2221 gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt
     2281 atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaa
//
LOCUS       DACCXW010000216         2263 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCXW010000216 DACCXW010000000
VERSION     DACCXW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.225"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..969
                     /locus_tag="IVY71_002882"
     CDS             <1..969
                     /locus_tag="IVY71_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7986871.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="IVY71_002883"
     tRNA            1159..1232
                     /locus_tag="IVY71_002883"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
     gene            1909..2263
                     /locus_tag="IVY71_002884"
                     /pseudo
     CDS             1909..2263
                     /locus_tag="IVY71_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          777 a          398 c          426 g          662 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta gactagacag
     1861 aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat gtcaagacgt
     1921 caacgaagaa cctattcaaa agaattcaaa caacaaatcg tcgatctcta tctcgctggt
     1981 aagcctcgcg cagaaattat tcgagagtat gagcttacgc cttcttcttt cgataaatgg
     2041 atgaagcaag cacaatcaac gggctcattc aaagaaagag acaacttaac accagaacaa
     2101 gcagaattga tcgcactaag aaagaaaaat aagcaactcg aaatggagaa tgatatttta
     2161 aagcaagcgg cgctgatatt cggacgaaaa gacaagtaat tgatgccaac aagcataaat
     2221 attccatatc agcgatgtgc aaaattctaa atatttctcg tca
//
LOCUS       DACCXW010000217         2155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCXW010000217 DACCXW010000000
VERSION     DACCXW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.226"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            26..466
                     /locus_tag="IVY71_002885"
     CDS             26..466
                     /locus_tag="IVY71_002885"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7986872.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            594..1277
                     /locus_tag="IVY71_002886"
     CDS             594..1277
                     /locus_tag="IVY71_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7986873.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1586..1659
                     /locus_tag="IVY71_002887"
     tRNA            1586..1659
                     /locus_tag="IVY71_002887"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1620..1622,aa:Arg,seq:tct)
     gene            1818..>2155
                     /locus_tag="IVY71_002888"
     CDS             1818..>2155
                     /locus_tag="IVY71_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HAP7986874.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLA"
BASE COUNT          711 a          340 c          445 g          659 t
ORIGIN      
        1 tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga ctccttatat
       61 tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat atgtacgacc
      121 gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa attcaggaaa
      181 aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt tttatacaaa
      241 agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata ttatcggtag
      301 tcccatcttt cagattccat atattggact agtcctttca aaaacggcat ttgcatggat
      361 tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat ggaatacctt
      421 tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat gttaaaaaca
      481 taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa aacactcatg
      541 cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt agtatgcgca
      601 atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg cgtacccagc
      661 aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta gcagaagtac
      721 cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag cttcagcctg
      781 aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg gccacgacaa
      841 tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc gagaaagcta
      901 aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa ggtcaagcag
      961 cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat agtgcaaaat
     1021 caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt ggcgttttga
     1081 tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag attccccgtg
     1141 gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg gatcatcccg
     1201 atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca aaggaatcag
     1261 aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt gaatcgaggg
     1321 ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag gatacaggaa
     1381 atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat tgataacctt
     1441 agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg ggccaaaata
     1501 attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt aattatctat
     1561 aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag catccgcctt
     1621 ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg ttagaaactg
     1681 aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc aaaggcaaat
     1741 aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata aaactcatca
     1801 gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta gttgcaattg
     1861 aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg acaagtaggg
     1921 tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg tttaagaacc
     1981 aaggagtgta caatggacta ttaggagtgg gactacttta tggagcttat ctttcttccg
     2041 catctaaaga aatcacaagt atgctattaa tatctatttt ttttgttgca ttatatggca
     2101 gtttgactag cgatcgttgg attttactca aacaaggtgg gctagctctg ttggc
//
LOCUS       DACCXW010000218         2118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCXW010000218 DACCXW010000000
VERSION     DACCXW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.227"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..293)
                     /locus_tag="IVY71_002889"
     CDS             complement(<1..293)
                     /locus_tag="IVY71_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7986875.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRW"
     gene            complement(265..1464)
                     /locus_tag="IVY71_002890"
     CDS             complement(265..1464)
                     /locus_tag="IVY71_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7986876.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1594..>2118)
                     /locus_tag="IVY71_002891"
     CDS             complement(1594..>2118)
                     /locus_tag="IVY71_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7986877.1"
                     /translation="NYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKK
                     EIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIA
                     KQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFN
                     ILRRNPKELLIDNN"
BASE COUNT          677 a          414 c          355 g          672 t
ORIGIN      
        1 aaccagcggt aagcgacatt ggtatcgact tccttaatgg tttgtcgcat ggaacggata
       61 ccgaataaat actgaataaa caccaatttg atcagaatca ctggatcgat acttgggcga
      121 cctaaatcgg agtaagtggc ttcgacgatt ggataaataa attcaaaatc aagcgcggca
      181 tcaatctttc gaacgagatg atcttgagga actagatcat caatcgtaag catcgcaaat
      241 tgatttctac cattcgtatt gtttttagac atcatagcta tcactccttg cattcgtttt
      301 cataaatcta ttataaaaga aaagactgta gacatcatcc ataaaatgga ctttgtctac
      361 agtctgcgca ttctcattag agaacactcc cttttttgtg acttttctga agctagagtg
      421 aatactttca actgcattag tagtgtacat gatttttcta atggcgctgc cataatcaaa
      481 gagttgttca acatgagaaa agtttcgttt gcatacatct actgcaccag aataatgaga
      541 ccaccgattt tgaaaactgt cgaaggcagc gtgtgcagcg tttaaagaag aagcactgta
      601 gaattttttc atgtctcgac agacttcctt ataatcctta cttgggaggt atcgtaacgc
      661 atttcgaaca agatgaacaa tacagcgctg cacaatgaca gaaggaaaaa ttgcttttgc
      721 gccttctttg agaccggaaa caccgtccat cgaaatgaaa aaaacatctt ctacaccacg
      781 tactttcagt tcatcaaata cttgcatcca gcgattttta gattctgttt gattgagcca
      841 taggcctaaa atctccttgt ttcctttaag atcatagcca agaatggtgt acacagcata
      901 ttctttagct tcatagtttt cgcgtaaagt gacgtacata caatcaacga atagaaatgc
      961 ataacacttg gctaatggcc gagcttgcca ttcttccaat tcaggaagga cggcatccgt
     1021 gatatcagaa atcatttcgt gggaaatatc aaagccgtaa atatcttcaa cggttgcggc
     1081 gatatcgcat tgactcattc ctcgtgcata catggaaaga accttacctt cgatgtcgga
     1141 gacatctcgt tttctattag gaatcaactc aggttcaaaa gaagcctccc ggtctctggg
     1201 aacatcaatg tttactttac caaagctcgt tttaagcgtc ttattgccat aaccgtttcg
     1261 tcgattgtca tgtttctttg gctctttaga atgggcgtca taccctaaat ggttattcaa
     1321 ttctccttga agcatttttt caaataaggg accaaacaca tcttttaaaa catcttgcat
     1381 atcctctaca gattcgggtt gataagtatt caaaatggat tcagctaact tctcagcatc
     1441 aggatttctt ttctttctag ccatcgtgta atcacctctt gatcttattt tagaaaaata
     1501 aaaaccgtga agcaaccacc cgcctaggat tgatggttac ttacacggtt tatattacac
     1561 tctcattttt ggtgctaaaa agcaccgaaa attttaatta ttgtcaatta ataattcttt
     1621 tggattcctt cttaatatat tgaatgaaga aataatcaat gatacaagaa gtactaaact
     1681 cataaatata actacataaa tcatgaattt aggtaataca ttgatatcta aacttgataa
     1741 tgtcttgtta aatccttcag cttctgcccc accacctaat tggctagatg cagattgttt
     1801 agctatttgt ttagctatat caccagtaac cttattcaat atattattcc caagcttgtc
     1861 agctgtatat tgagctaaga aataggaacc aacgaatgcg gggattgata taaataccat
     1921 ctcaattagg aattgaccaa aaatttctaa ttttgatata cctaatgata gtaatactgc
     1981 tatttctttc tttctagcat tcatccataa gaataataat aatgaaacta caactcctgc
     2041 aaatattaat gagccaaaaa ataatttatt tgaaattgag taaatacctg atatagattg
     2101 ttgtaatgca gggtaatt
//
LOCUS       DACCXW010000219         2107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCXW010000219 DACCXW010000000
VERSION     DACCXW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.228"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(124..1050)
                     /gene="cysK"
                     /locus_tag="IVY71_002892"
     CDS             complement(124..1050)
                     /gene="cysK"
                     /locus_tag="IVY71_002892"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7986878.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1190..2077)
                     /locus_tag="IVY71_002893"
     CDS             complement(1190..2077)
                     /locus_tag="IVY71_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7986879.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          573 a          478 c          359 g          697 t
ORIGIN      
        1 aaacagtcgc gttcactatt ctacgtctct attcaaataa tttttctcat cataaatttt
       61 tgtgtgttct tttcatcttt aaaagttttt tacataaacc tatctggaga aaaaccaagt
      121 gccttatatc tcttggtaaa gggctgtaga taaataacgt tcaccattgt cgggaaccac
      181 agctaatacc ttcttgcctt ttcctaattc ttttgcgact tttagcgcgg cagcaattgc
      241 agcgccagat gagatgccta ctaaaatccc ttcttttcgc ccaacttctc tagctgtttc
      301 catcgcttcg tctccactaa tagaaaggac tttatcatac acagaagtat ccagtgtttt
      361 aggaacaaaa cctgtaccga ttccttggat tttgtgaggt cccgcttctt tcccttctaa
      421 aatagcagat tctgctggtt ctaccccgat taattcgatt ttcggataca cacgttttaa
      481 ttctcggcca gcgccagtga tcgttcctcc tgtaccgata cctgcaacaa acgcgtccaa
      541 gccatttact ccaaatgctt gatgtatctc aggaccagtc gtcttctcat gcacaagagg
      601 atttgcttca ttttcaaatt gtaatggtaa aaagtagccg ttttcttttg ccaaacgttc
      661 tgcttctctg attgatccag aaataccatc cgcaccagga gtcaaaatca actctgcgcc
      721 gtaccctttc atcaataaac gtctttcgat actcatcgtc tcaggcatca cgatgattac
      781 tttatatcct tttgcaactc ctaccatcga caatccgatt cctgtatttc cagaagtagg
      841 ttcaatgata gtatcacctg gtttcagtag accatcgtgt tctgcttttt ctatcatact
      901 taaagcaata cgatctttta cacttcctcc tggattaaaa aactcaagct ttacaaaaac
      961 atctgctgaa tcttctggta cgattttatt taatttgacg attggcgttt tgccaatcaa
     1021 ttcggtgact gagttataga tagctgtcat cttcaattcc tccttgtctt tgctcttctt
     1081 acttatcata atacaatctg ttcatttatg gcgagtgatt cgtctttgac tagcaaaaaa
     1141 aagacaaaga tataccatat atggtaattt ctttgccttt ttatctaaat caaacgttta
     1201 gttcttgacc aactaataaa gaataactgg acatagacat gccatttttc gccattagat
     1261 catcaacaga catgccaaat ttttgtgcaa tatcccatag cgtatcacct gacacgactg
     1321 tatatgtctg accattattt gtatttacat ctgttgttga agatgatgac gttgaagtcg
     1381 tcgtagtagt cgttgttgtt gtacttgtgg ttgttgtagc cgaacctgtt gatggcgtat
     1441 caaaacgggt taaattataa gtattaatca atgagttcaa tttggcccca taatttggat
     1501 cggtagcata acgacctgtc aagtacgctg ttgcatcata aaaacttgtt gtattgcttt
     1561 tccacacgcc agcataatga taatcgcccg tggaaaaact ggttgaacga agaacattcg
     1621 catggtcttg gaaggattcc caataagaag aatattttcg gaatggctca ttcatcgtta
     1681 cccattgtcc gtttacatat tctttggttg ccatataaac gacctgtccg ttatagcttc
     1741 ctttcacacc gaatagatta taatttggcg cactagctaa tccggaagtg ccatatgcac
     1801 tttccaacaa cgcttgagcg atcatgactg aagcatacaa gtcattactt gcagcaactt
     1861 ctgacgcaga ccagccgatt tgatcgataa acgcttgagg atcagcgact gtttgagatc
     1921 cttgttcatc tgcatgaaca tgttgcggta ccgctgtaac catcatcggc ccaactaaaa
     1981 gactcgttcc aattaaagga gcaatcgctt ttttattgat caccattttt cttttaggtc
     2041 tatggtgccg ggcactgcgg gtcttcagct cttgcattca aattcctcct caaaataaaa
     2101 aaactta
//
LOCUS       DACCXW010000220         2098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCXW010000220 DACCXW010000000
VERSION     DACCXW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.229"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..633
                     /locus_tag="IVY71_002894"
     CDS             <1..633
                     /locus_tag="IVY71_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HAP7986880.1"
                     /translation="KLIKWLILAVIGIYILNVITKARDGGGLSKLTLKDFTSVFDSDD
                     SPLVDSFFEMGGTMSIITFFLISGNGIYPFANTYLTSLLGSISTRMLSMFGIKFVLLA
                     DWFSQDYLGITWGTGFSMIAEAYVNGGYIGGIVYIFIIGIVIGKILSNSIDNQDIENH
                     PMKMFLAITTANIIMGFPRAASYLIVKNFVYGVLIILLINFFLQQFNKRL"
     gene            679..>2098
                     /locus_tag="IVY71_002895"
     CDS             679..>2098
                     /locus_tag="IVY71_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HAP7986881.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFI"
BASE COUNT          676 a          288 c          368 g          766 t
ORIGIN      
        1 aaattaataa agtggcttat tttagctgtt ataggaatat atattctaaa cgtaataacg
       61 aaagcacgtg atggtggagg actaagtaaa cttactttaa aagattttac atctgtattt
      121 gattcagatg attctccttt agttgattct ttttttgaaa tgggggggac tatgagtatt
      181 ataacttttt tcctaataag tggtaatgga atatatccct ttgctaatac ttatttgacc
      241 tcactcttag gctctatatc aacaagaatg ctatcaatgt ttggaataaa gtttgtttta
      301 ttagctgatt ggttttctca ggattattta ggaatcactt gggggactgg attctcaatg
      361 attgcagaag cctatgtcaa tggtggatac attgggggta tagtctatat ttttattatt
      421 ggtatagtca tcggtaaaat cctttctaat agtatagaca accaagatat tgaaaatcat
      481 cctatgaaga tgtttttggc tataactaca gcaaatatta taatgggttt tcctagagct
      541 gcttcatatt taattgtgaa aaattttgtg tatggtgttt taattatatt gttgataaac
      601 tttttcctac agcaatttaa taaacgtctt taatatttaa gcttaataaa aagataaaac
      661 aagtcaggag ttgcaaaaat ggaaagaact gctcggctca aaaaaaacac tatttgggct
      721 ctggtgtttc aaataacttc tattgtctgt ggtatgatct tgctacgttt gatattaaca
      781 aagtacggat ccgaggttaa tggaatgatt agctctatta accaatttct acaaattatt
      841 tcttttttac aattaggagt aggcgctgta atgcaatcaa ctctatacaa accactagca
      901 gaaaaggata atttaatgat aagtcaaatc ttagcttctg gagggaagtt ttatcggcaa
      961 attgcatata ttctactagt atacgtgggt ttgttacttg cagtctatta tgttctcgtt
     1021 cctaatgaat ttgggagatc atatacaatg gcaatgattg caacattaag tattaattca
     1081 tttgctcaat attattttgg tattgttaat actttgttat taagtgctga ccagaaaatt
     1141 tatattacgt atattgcaca gacaatagca tttatttcta atacagttgc atgtgcttta
     1201 ttaattagtc tgaacacaga gatccttatg gtaaaactta ttactgctat aatttatctt
     1261 gcttgtccaa tatattatag atggtatgtg gatcgaaact ataatataaa gaggaatgtg
     1321 tcatatgata aggaaccgat ccctcaaaaa tggaatgggg ttgcacaaca cgtagcttct
     1381 gttgtactag atagtactga tgttgtagtg ttatctatat tttctaacct gatatatgta
     1441 tctatatata atgtgtacaa tttagtaata tacggagtaa aacagctatt catggctatg
     1501 acaaatggcg ttcaggcctt actaggggaa ctttgggtca gaaaagaaat agaagttctt
     1561 tacaaaacat ttgcatggtt cgaatggatt gtacatactt tgacagtttt tatttttggc
     1621 tgtaccggta tactaatcgt tccatttatt caagtatata caagaggagt aacagatgct
     1681 aattataatc aattatcatt cgcagcttta cttgtaactg ctaacgcact tcactgtctg
     1741 cgtacacctt atcatgtaat gataaaagca gctggtaaat ataaagaaac tcaaaatagt
     1801 tttataattg cttctttgtt aaatttaggt ttatcattaa ttttggttaa accactaggg
     1861 ctagttggag tagcattagg atctctaatt tcaatcacat atcatacact ttggagtgca
     1921 aattataatt caaaatattt gcttaaatgg ccgatgaaaa aattctataa acagttattt
     1981 gtaaacatct taactgtagt tctttctttt ggggcgacat cacaattgaa aatgatgtca
     2041 atcacatatt tggattggat tgttctcgca attaaatgtg cagttattgt atttatat
//
LOCUS       DACCXW010000221         2088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCXW010000221 DACCXW010000000
VERSION     DACCXW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.230"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(131..418)
                     /locus_tag="IVY71_002896"
     CDS             complement(131..418)
                     /locus_tag="IVY71_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986882.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(589..1941)
                     /locus_tag="IVY71_002897"
     CDS             complement(589..1941)
                     /locus_tag="IVY71_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP7986883.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          575 a          417 c          359 g          737 t
ORIGIN      
        1 aaaaaaaggg gttgacaaag cttgtcataa cccctttttc tgattaaaag atagtaaaga
       61 gacagtcatc ggtaattgtg atgatttttt gtcatcactt aaggatgagc ggccttttca
      121 aaaactgtct ttaaagatca tcatcagttt ctagcacata tggctgacct tcatatgtgt
      181 actctgtatt caatgtgccg tcattttcat ctgagtagat gcgtctactg aaaggaaaac
      241 gatgagaaac attatctttg gcgtcatttg ctggcatcgt gtaatattcc tcttgcgttt
      301 cattcaactg agagagtact tgatctgcca actcatcaat attgatcgtt aatttcttcg
      361 aagcactttt ataatcttct aaaaatgcat cttgttggac aaaatattct ttattcatct
      421 tctttctcac ctttcttttc aaaatcaaat aaagctgctt gttcttttat gtaacagctt
      481 tatctttgct gcttattgtg acaaaaaatt cgaagaaaga caactaattg tacttttcac
      541 aaaaaaagac agagggtcgt gacaaaattg tgtcacaacc ctttttcttt attgtgcagt
      601 ttcgatttcg cctagcgctg cttgtgcaag taggttcaag taattccatg ggcggtcaaa
      661 atgtggttgg aagaagaaat ctgaaagtgc gagatcttct acagtcattt tattttgaac
      721 agctaaagac atggtatttg ctgattgtgt aatgtcgtat ttggacatga actgagcacc
      781 tacgatacga ttcgttcctt tttcatagac tagctccatc attacttttt cagtagttgg
      841 catgaactct ggacgataat tatcttcaaa tacagtcgct tggacttcta gatcgttcaa
      901 agttgcgctt tctttggtca cacctgtcga accgatcgtc cagccaaata ggtaaagtcc
      961 tgaagttcct tgagtaccac gatacgccat tttatgatct gtcaagttat ttcccacaag
     1021 caaaccttga cgaacggcat ttgttgccaa aggaatatag ttttgcgtgc cacttggatt
     1081 gtagttgact actgcagagt ctccagctgc aaaaatgtct ggattacttg tctgcatgta
     1141 ttcattcact ttgattgccc cgtttggcag tgtatctact tttccttcga tcagttttgt
     1201 atttggacgg aagcccacac ataaaatgac catatctgta tcaaatgtat ctgttggtgt
     1261 agctacttgt ttcacttttc cttcttcatc tgtgataaat tcagtaacat tttcgcctag
     1321 cgcaagagta acaccgcggt ctgtcaattc tttttctaag atatctgtga atggtttatc
     1381 tagatattta ttcaagatgc gatctaatcc atcaatcaat gtcacttgtt ttccagattc
     1441 gacaaaagct tctactaatt caataccgat atatccacca ccaacaatta ctacacgttt
     1501 tgcttctttc gcttgtgcaa taatttcatt tgcttgattg tagtttttgc ataggagtac
     1561 atttttcgca tcgattccac taatcggtgg gataataggc catgaaccag ttgtcatcac
     1621 taatttatca taagaaaccg tttctgtttc tcctgttttc agatcttttg ccgttactgt
     1681 tttattttct gtatcgacgt ctgttacatc gtgttccatt ttaactgttg ctcctaatga
     1741 agataattct tcaggatttg agtagaacaa gcctgctggg tcctttacta ctccgccgac
     1801 ataaagtgcg ataccgcaag ataagaaaga aatattgtca tttctttcat atactgtcac
     1861 ttctgcttct ggatgatttt ttaaaatact ttttacagct gcagtacctg catgagtaca
     1921 gccaattact actactttca tttatggtcc ctccgattaa cattttgtcc ttcgacaaaa
     1981 ctactataac agaaataaaa cggatacaac agtttaatgc ttgtgaattc acaaaggttt
     2041 gtttgtagtt tcattccttt tcaagcataa gaacccaata tctacaag
//
LOCUS       DACCXW010000222         2065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCXW010000222 DACCXW010000000
VERSION     DACCXW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.231"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(235..1236)
                     /locus_tag="IVY71_002898"
     CDS             complement(235..1236)
                     /locus_tag="IVY71_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP7986884.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(1395..1661)
                     /locus_tag="IVY71_002899"
     CDS             complement(1395..1661)
                     /locus_tag="IVY71_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986885.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1651..2007)
                     /locus_tag="IVY71_002900"
     CDS             complement(1651..2007)
                     /locus_tag="IVY71_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7986886.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          621 a          383 c          245 g          816 t
ORIGIN      
        1 cgtccttatt taaaggcatt taacattgac aacttagtat aatgtatctt atgtaaacta
       61 ataagatttg aattttattt tatacacact cgataataga tcaattttat ggtcccccgc
      121 atttacgatt gcacaagaga aacataaagt cgacctatta gcatttcttt tcaattcaaa
      181 cattaaaaaa gtttatacct ctgctactaa aaaatgaaat tatctattta aaatttaaaa
      241 cggtaaatct gattcatcca aattggaaaa tggatcagcc tgtattggag ctttgtattt
      301 ctccataata acagatatct ttttttcatt ttttaaaaaa taccttttaa atttcttata
      361 gtcctctgaa atcaaaatat agcgtgatgg atactcatca aaagaagaat cgtattcttc
      421 ctttaaaatt cccgatttac ttaatttttc tagtacatca aatactctat cactcaagta
      481 actccttaaa ttgttatcac tttcccattg aactactttt ctataagttg aattatcagt
      541 tgaaaagata gaattttcat ccataaacgc atatattagc agcaatcttt catcattgtc
      601 aaattctatt ggatcaaaaa tatctaagtt gtcatcaaaa tctttatcag ttccggtaac
      661 ttttttgtta attactgaag gtgtcttgta agaaatagaa tctaaatcaa agcgttttga
      721 ttctaaatta ttttgtgctt tctcaattaa ataattcact ttttttgtga gaaaccttct
      781 gatattgtct ttgctatatg ttccataaaa acccttatct tctaaagagt ccctgtactt
      841 cttaacttct atatactctt cagccaaaac ttgtggaact ggttcatcta agaaaaaaac
      901 ccctacctcg cctttgctat ttttataaaa gtaatcaatt tctgctatag ttcccgatct
      961 ataattatca actggagaac ccaacttatg gccaaaaatc gctaataata tatcactatt
     1021 ttctacaatt tgtttattaa taacactttg cccagagcta ttatttatat aagtaggaat
     1081 cgcattcgtt tcccatcgaa ttggcatcaa aattactttt gtattatctg tattttctat
     1141 attccatcta tatatggttt cttctacttc attcctcttt gctgctatat cacctggtga
     1201 tgctataaat acttttatta gatttatggt tttcatttat tgtacctttc tctttatgtc
     1261 tctttaaagt aatttttgta taaaatttat tgacttttac actataggac aacttttatc
     1321 tatttagtac aaaaaggccc actaaactaa ttgattagtt aggagcctat tacaggtata
     1381 ttttcaactc gcttttaagc aaattctact accagctttt tgcctaatgc cctagcgata
     1441 tcatcaagtt tatcgaaaga tacgttatcc cctctctcaa ttcgagcaat agtagattga
     1501 gttgtatgtg ctttttgtgc aagctccgct tgagttaggc ctgcttcctc ccgtgcatga
     1561 taaagagcaa ccgcagtttc gagttttctt ccttcacggt catatgcttc tgcaaaatct
     1621 ttgtcttttt gcttttcttc aatcaatttt ttaatcatca tttgtgaacc actcctctct
     1681 aagttctttt gcatgtttaa gttcacttat tggtgttttc tccgtttttt tagtaaatcc
     1741 atgggtaatg acataccttc cattgaccac atggaaatag atagctcttt ggatgtttga
     1801 tcctacctta cttcttaatt cgtacaaatt agtgtctaat tttttcaccc atttcaaccg
     1861 ttgagctact agcatcccat tcttttctgt ttcttctatt gtacgtaaaa gcttcgcact
     1921 atcttttttt ggtaatgttt ccaaccattc taaaaattca tcatgaccat taggtctttt
     1981 aaatgtttca aaagatattt tttccatacc caattatatc atatattgat tttatattat
     2041 agtatataac ataaaaattc gaatt
//
LOCUS       DACCXW010000223         1999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCXW010000223 DACCXW010000000
VERSION     DACCXW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.232"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..511)
                     /locus_tag="IVY71_002901"
     CDS             complement(<1..511)
                     /locus_tag="IVY71_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7986887.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSL"
     gene            1164..1259
                     /locus_tag="IVY71_002902"
     CDS             1164..1259
                     /locus_tag="IVY71_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7986888.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1561..1806)
                     /locus_tag="IVY71_002903"
     CDS             complement(1561..1806)
                     /locus_tag="IVY71_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986889.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT          634 a          397 c          309 g          659 t
ORIGIN      
        1 ttaagctcct aaactcttca atacgaacta cataataacc agtagaacgg tactgcataa
       61 gcagtcggaa tagagttttt gcatacgtag aacgcaacga cgtgaaagca gataattcaa
      121 atctactaaa ttcagctgtt aaaccattaa taatatattc taggtccgga ttaacactga
      181 cttcaacaaa cttttttgtt ttattgattt caaaaccagt aaacaaaaca aagtagcgaa
      241 tagaatcctc atcttcagta cggtaagtaa gattaagcat atctttatag agcttttcta
      301 aatcaccgac aaaggctttt gtagcagtca tcttataatc acttaactct tttaattcct
      361 cgaaagaaaa tctaactttt ttcaaaccac gatctttcat acgagcgcat aatgcaaaaa
      421 ataaatccat ctgcttagca ttgaaatttt tcagcggtac taaattcaat tcattttgat
      481 atttgactgt taattccgtt ttttcatcca ttcctttctt ccccccttac aaataaataa
      541 taacctttaa caaccaactc gtaaataggt tggttgttat tatacaaaag gatattagta
      601 ctcagataaa aagttgttag tactcagata aaaagttgtt agtactcaga taaaaagttg
      661 ttagtactca gataaaaagt tgttagtacc cttctgtatc cgttgggaga gtaaggctca
      721 actgccccct aaaaagggtt ctaaaaaatc tttaaaaaat aaataaaata aacgcgcgcg
      781 cgcgagcaaa atttcccgcc aaaaaatcac ccacttttta aaataaacag gttcgcgcat
      841 gatattaaac tgccaatcta gctccctatg gtcgctgtaa gaaggagtga tcgctacgct
      901 ctcacatttt acttgcagca gtcgccttta ggctccttcg ccaaaaataa ttcattaggg
      961 actcctttgt ggcgtcctga gagctcgtct aagccgttct aagacaagat tcgataaaaa
     1021 ctatgtcctt cgttctaaag cgccttacag gcggtcaggg caagccctaa caaccccaag
     1081 gcaataaaaa acttaggctt atttttgctt ttttattgta taattgattg tcgcctatcc
     1141 gacagtcttg cgactggaag gaggtgtgtt tcgtgttcac taactttatt tgctcccttc
     1201 ttgttgggct tttaagcgct ttgttcagtt actggttgaa taaccgaaac aagaagtaaa
     1261 acttaattaa ggcgactcag cccacaaaaa aagccccaga gaatccgacc tctctggggc
     1321 tttcgtgtgt ttcgtgttca cttatttgca atcaaattat aacagaaata aatatttatt
     1381 tcaaaaatat cctacaaaag tctaaaatcc gtaattattt cttatatgca acatttacaa
     1441 ataaagtcgc tctgagagct cgtatttttc gatttaagac gttttattta gtttttggtg
     1501 tatttcattg gtccaagcta aaaaactctt agaagcgatt ttagagccat ttaaacccac
     1561 ttaattatat tctccatgaa attcgctcca tgcgtcaaaa gctgcgtctg aaattccgcc
     1621 gtcttcgttc actagttgca ttaatttttc caacgatcgt tttctttcgg gtgtcatatt
     1681 tttatattcc aacaattttt cgtctgttaa atcgaacttg attgcttgaa ttgcttgctc
     1741 taatttttct aagttttgaa aatattcgta aggaaatttt tccatttttt ctttctccag
     1801 tctcacaatt tttctagaaa taaaatcact tctttgccgt ctatactctc aaaaaaaatg
     1861 tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt atcgattaca
     1921 cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc actttctaaa
     1981 aaaagtgggt agataggct
//
LOCUS       DACCXW010000224         1983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCXW010000224 DACCXW010000000
VERSION     DACCXW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.233"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            596..928
                     /locus_tag="IVY71_002904"
     CDS             596..928
                     /locus_tag="IVY71_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7986890.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1547..1888
                     /locus_tag="IVY71_002905"
     CDS             1547..1888
                     /locus_tag="IVY71_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7986891.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          688 a          265 c          421 g          609 t
ORIGIN      
        1 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
       61 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
      121 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
      181 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
      241 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
      301 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
      361 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
      421 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
      481 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
      541 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
      601 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
      661 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
      721 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
      781 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
      841 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
      901 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
      961 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     1021 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     1081 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     1141 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     1201 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     1261 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     1321 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     1381 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     1441 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     1501 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     1561 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     1621 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     1681 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     1741 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     1801 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     1861 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     1921 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     1981 ttt
//
LOCUS       DACCXW010000225         1949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCXW010000225 DACCXW010000000
VERSION     DACCXW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.235"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            31..1200
                     /locus_tag="IVY71_002906"
     CDS             31..1200
                     /locus_tag="IVY71_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7986892.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1467..>1949)
                     /locus_tag="IVY71_002907"
     CDS             complement(1467..>1949)
                     /locus_tag="IVY71_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986893.1"
                     /translation="NYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQ
                     LLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNAT
                     VLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE
                     "
BASE COUNT          691 a          332 c          298 g          628 t
ORIGIN      
        1 taaaaaagaa aggagactaa ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga
       61 aaatattcat ttttaatgat cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga
      121 actattcttg gatggcaatt ttacaataat gaacactctc agatagttgc ccatgctcaa
      181 tcagctcaaa aaaagttgat actgactacc aatgaacaac agctcgttga aaaaatcgat
      241 caactattaa gaaaaaatga ttatattggc tcaatttacg taaagaaaaa taatcgcatc
      301 atcatggaaa aaggctatgg ctatgcgaat aaactcacgc aacaaccaaa tgatccttct
      361 ctttattatc agattggttc ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa
      421 gtcgagcgtg ggcacctttc tttagatact aagctggctc aattctatcc acaaattgct
      481 ggcagccagc aaattactat ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact
      541 gcttctccaa ccataccgat gactgatgaa gaagtcgttc aatttgcgat tcagcactta
      601 aaatttacag attatcagac ctctcattat gattcattga actttaccat attgactgga
      661 attttgatca aattaacaaa tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa
      721 cctttaggat tacagcatac aaaattttat gaccaagtca aaaattcatc caatcatgca
      781 ctatcctatc aaatgtctaa agaggatgat tactttaaac cactgacaga atctgaaaca
      841 gagatccata acgaattagg gactggtaat atcagtatgt ccgtctatga tttaaatacg
      901 ctgtttacac aagttttatc tggaaaacta gtttccaaag acttactctt ctcattatgg
      961 cgtgaaaatg cgaatggaca tccatatagt ggtggggtat atagcggaga taattatatc
     1021 ctgtcgcaag gaaatatcga ctcttttgat acatctgcta tatttagcaa agattcacag
     1081 gatgctatca tcatggaaag caatataaaa gctgacaaaa ctatcaaact gcctactgct
     1141 gatctaagaa accaaatcta tgacctgatg gaaggaacta ctaagttggc aaatagctga
     1201 tatgatgaaa agatagcaaa tactacttct ttgaatagca tttgcttaaa aaaagtactc
     1261 gactaaacgt aggaaagagt gtgggacaaa tctaaaaatc aatttttgct acagacttta
     1321 attccaaata aacagaacag aaagaggtgt tcttctttct gttctgtcca aattattcta
     1381 agatttctct ttaccaacat tatttgacaa agggcctatt aaatctaaaa tagtatcttt
     1441 agattgctaa aatatgtgct agcaaatcat tcatggcttg ttcttatttt taattttatg
     1501 caataaaaaa gaagaacaat tagcgtgatt atagaaataa ataatgagat tttagaaaaa
     1561 aatgtgtatg aatacctgat tgttacttta tttattccac tttcactttt tactgtaaca
     1621 aaaggacctc cttgttttag tactgtagca ttttttgaat taacagtaac cgttaatcct
     1681 ttataccgga atacaggtaa aactattgtt ttgccttttg aagatttaaa cgtaaatgta
     1741 atttcatctt tatttggaga aaagctacta taaataactt gaccatcttc tgtaacttga
     1801 tggtttttca aaagcaattg atctttttta tagcctatag gtacataatc aaaattctca
     1861 aattcttcaa gtttattttc catttgttca ttagaataag ttccttgaaa attcatttct
     1921 ttcaccatgt taactactga tgaataatt
//
LOCUS       DACCXW010000226         1901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCXW010000226 DACCXW010000000
VERSION     DACCXW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.236"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..505
                     /locus_tag="IVY71_002908"
     CDS             <1..505
                     /locus_tag="IVY71_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7986894.1"
                     /translation="INELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            495..1637
                     /locus_tag="IVY71_002909"
     CDS             495..1637
                     /locus_tag="IVY71_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP7986895.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1650..>1901
                     /locus_tag="IVY71_002910"
     CDS             1650..>1901
                     /locus_tag="IVY71_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7986896.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNL"
BASE COUNT          672 a          253 c          394 g          582 t
ORIGIN      
        1 aataaacgaa ttgaatagct gggcggatag caaaaaaaaa atctataaat ttattgtagc
       61 tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa ttgaatatgc caagtgagca
      121 atatggaata gctgttcatc caagtgctat agtggcaaaa gaaatttcaa ttggttatgg
      181 aactgttatt tttgcgaatg ctgtaattaa cccagatgct gtaattggag agcatgctat
      241 aataaacaca ggtagcattg ttgagcatga ttgtagaatt aataactatg ttcatttatc
      301 tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta ggaactcaaa taggaattgg
      361 ttctcaatgt atccagatga agaaaattgg atcttggtgt atgataggcg cagggagcac
      421 tattgttaag gatattccct cacattcctt agcttatggc aatccagcta aaattaagaa
      481 agaaggaatt aattttgaat tctagaattt tattatcaag tcctcatatg ggaggaacag
      541 aagaaaaata tgttgctaag gcttttaaag ataattggat agcgccatta ggtgaaaatg
      601 tggataaatt tgaagaaagt attacaaaat atactggttc taagggcgga gcttctgcgt
      661 taagttcagg tacagctgcc atacatcttg ccttaagact gcttaatgtt ggaaaaaacg
      721 atattgtctt tgctccttca tttactttta tagctacagt aaatcctatt ttatatcaag
      781 gggctgtacc agtgttcatt gactcagaac ctaatacatg gaatatgagc cctgttgcac
      841 tagagagcgc attagaagaa gctgaaagaa acaaccaatt acctaaggca atcatcgtag
      901 tgaatttata tggtcaaatg gctgattatg aacgaatttt agctattgca aaccaattta
      961 atgttccagt gatagaagat gcagcggaat cgttaggttc tacttataag ggaatttcgt
     1021 caggcttatt tgggacaatt gggatctatt catttaacgg taataagatc atcacaacct
     1081 caggtggagg agctctagtg tctagtgata aaaaatatgt tgagcattca agatttttag
     1141 caacacaagc aagagatcaa gcaccgtatt atcaacatag cgaaataggt tataattacc
     1201 gtctaagcaa tatttctgcc ggtatcggac gtggacaaat ggaagtattg aatgatagag
     1261 tagctaaacg aagagaaatt ttcaatcgat ataaggaagc tttttctgga attgatagaa
     1321 ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg ttggttgtct actttaacga
     1381 tcaatgatta taattctatt gatcaagtag ttaacgtaat aaataaacta aatgaaaaaa
     1441 acattgaaac aagagctttg tggaaaccac tacatttgca accagtattt caaggggtaa
     1501 aaatgtatta ccatgataat aatcttagtg tgtcagaaaa gttatttaat agcggcttat
     1561 gcttaccttc tggaagtaat atgacagata gtgaacaaga atttgttatt gatgagttta
     1621 aaaaaatatt caagtaggtg tgatatttta tgaacatagg aattattggt ttaggttcca
     1681 tggggaaaag aagacttaga ctcattcaag aacactttaa tgatatttta ctatgtggtg
     1741 ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga atttggaata ccaacttata
     1801 atagtctaac agaggcaaaa aatttgtttg aaataaattc agtttttgtt tgtacatccc
     1861 ctataaccca tgaatttatt ataaaagagg ctttaaattt a
//
LOCUS       DACCXW010000227         1895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCXW010000227 DACCXW010000000
VERSION     DACCXW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.237"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(339..794)
                     /gene="tnpA"
                     /locus_tag="IVY71_002911"
     CDS             complement(339..794)
                     /gene="tnpA"
                     /locus_tag="IVY71_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP7986897.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1037..1895
                     /locus_tag="IVY71_002912"
                     /pseudo
     CDS             1037..1895
                     /locus_tag="IVY71_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          602 a          368 c          355 g          570 t
ORIGIN      
        1 atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aatccagtga ttttctagga
       61 agattatttc tgcgccagca ttttctaatg tttgaatttg tgttttgtaa tcttggttgc
      121 ttgtgctaat tcttgaatag ccgtaaattg ccaaaatttc atcacccttt ctggacataa
      181 gtttctgaca ccagttaaaa tcaagtctat cagtaaccca tacacccgtg aggacgggtg
      241 tatttttaat ccccctagaa ggggtcgaca ctcgctgccc cttaaaaggg gatttttaaa
      301 agaccgccaa ctgctcatct tcactcactt acttctcttt attttttaaa tggttctacg
      361 tactctcttt tacttataat atatcgaatg cgatcatcag cttcttgttc acggatgtat
      421 ttttcatttg tcttttgatt caatccgact gtactcacat agtatctctt cgaccaaaag
      481 gatttgtttc cgtatttata tttgagattt gcgtgctgct cgtggattag taccgcacta
      541 cgacctttca gataccccat aaaactggat atacatattt ttggtggtac tcgaacaagc
      601 atgtgaatat gatctagcat tgcatgtgct tctatgattt ccacatcttt catttcgcac
      661 aacttcctaa gtatttttcc aagatctttt cgtaattgcc catagattac ttttcgttga
      721 tattttggtg taaaaataat atgatactta caattccaac gagtatatgc taaactttta
      781 tcgtcatttg acatgaaaaa actcctttcg atctgatgag cggttggtag ccactcacta
      841 gtatatcgaa aggagttttt atcgctaaag atacttcttc cacgcgtaga aacgcgcggt
      901 tttcttgttt cgcacgcttt acgcacaaaa ctgactgaag tcattaagaa aataaaaggg
      961 tctaaaactt ttaagttttg taccgacccc caaaagttag accaaaaaat ctaacttttg
     1021 gaggtcggta ttttttatgg ctaagtatac ttttgaactt aaattaaaga tcgttcatga
     1081 ttacttggat ggtaaaggcg gaagtgatta tctggcaaag aaatattcca ttaaggctcc
     1141 atcacaagtg aagcgctgga tcaatgccta tcaagaattt ggtgaagagg gtctagtaag
     1201 aaagcgacaa aagaaaaaat attctgttca atttaagctg gatgcgatag agttatatct
     1261 aacaagtgag ctgtcctatc gtgaagttgc caacacactt aacatgaaca accctaattt
     1321 gatcgctagt tggatgcgtc aattccgtga gggtgggatc gatggactct caaaaacgaa
     1381 aggatgtcca cctatcttgt ctaagaaaaa tgaacccaaa aataagaaaa aatctacaac
     1441 taaagcaacg tctaaagaac gtgaacgcat cgaggagttg gagaaacgag tccgttccct
     1501 tcaaattgaa aatgcctttt taaaagaatt gaggaagctg cgaaaacagg aagcccaaca
     1561 acgacgaatg aagcaatcgc acgaatcatc gcaagcctcc gaggaccgtt taaattagtt
     1621 gagcttctag aaactttaaa gttccctaag gcaatcttta tgtactggca aaaacgattg
     1681 gaccgtaaaa atccagatca agagattgaa gcggaaatgc ttaaagttcg ggagaagcac
     1741 aaggattatg gctgtttacg gatgaccaat gaactaagaa accgcggttt ctctatcaat
     1801 aaaaagaaag ttcagcgcct gatcaagaag ctaggaataa aagtaacgac atacacacgt
     1861 aaatcacggc gctacaattc ttatcgagga caagt
//
LOCUS       DACCXW010000228         1886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCXW010000228 DACCXW010000000
VERSION     DACCXW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.238"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            10..>1886
                     /locus_tag="IVY71_002913"
     CDS             10..>1886
                     /locus_tag="IVY71_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7986898.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     T"
BASE COUNT          738 a          259 c          330 g          559 t
ORIGIN      
        1 gtgaagataa tgggcatcga atttagcgaa cggcaaatga aaatactata ctttttgatt
       61 gaaaatgagc acacaactta caaagaatta tcagaacaat tatatgtatc tacgaaaaca
      121 attagcaaag atattttaat tttgaaagaa ataatccaaa aaatgccaga tgtacaaatg
      181 ttggttcaac ctaaaaaagg cgtttcgctt attggaaaaa aagaaacact tcgaaattta
      241 ttatccagtc ataaaatcaa tatcgcagac tctccggagg accgtctaac ttatgtgttt
      301 gctaaattat tgaatactaa tgattctcta aagattcaaa atcttgctga cgaattatac
      361 gtgagcaggt cgacggtaga aagcacaatg aaagatgtta gaaaaagact aaacaagcat
      421 gggatgtcct tagtttcaga tcgaaatggc gtgcgcatca aagctgatga aaaaagtaaa
      481 agaaggttga tgtcagaagt tgtgaactat tattggggtg gctttacagc atctaacagt
      541 aaaacagatc tcttagactt gaatatcaat ttcattggtg aagcgaataa tttcttaaat
      601 aaggacatat tgaaaaaagt agccgaattg cttaatcgat acatgaataa gacacaattg
      661 aaatttacag attatgaatt tcaaacttta gctatccacc ttgctattgc gatagaaagg
      721 attgaaacaa gcaatttctt atcacgtagt aataattctg aagaagaaat ctcaaagaat
      781 acacttattt taatcaaaat gattgaaaaa gcattttcaa ttattgtgcc aatagatgaa
      841 caagcttatg ttgacattca cattacttcg attgaaaaaa atgctatgaa tcacgactta
      901 gatcaaacga agctttccta tttagatatg ggtgttgagc tacaagagtt agtaagaatt
      961 aaattaaatg aattttatcc tgatgaagaa ttgatcaata atctagtatt acatctcaat
     1021 gcagcagtta aaagactcaa attgggagta aatatctata acccttatac ggacaaaatc
     1081 aaatattctt ttaaaaggtc atttatgata agtgtagatt tattagagga aatcgaagag
     1141 agattctgta tacattttaa tgaagatgaa atcgcttacg taacactcca tattcaatca
     1201 cttttggatc gttacaaacc tgacaaaacg aaagtgatat tagtttgtag tagcggatat
     1261 ggaacatcaa aattattaga gcaaagaata aataacggtt ttgcagatat ggttgagata
     1321 gaagatgtac taagcatcaa tgaactacaa gatagaaaag tcactgatga acttgttata
     1381 agcacgattc cgattgaaaa tacaaatttt cctgttattg ttgttagtcc tttgatgtta
     1441 ggggcagatg tcagaaaagt aaaacagtat ctacagttaa atgaacaaac agaagatgct
     1501 tttctaaatt tattagataa acgattttgc ttttttaccc accaaataaa ggataatcaa
     1561 gtgaacgttt taaatataat taccaaaaaa ttggtaaaag aaaattacgc gaaagaaggg
     1621 atactagagg gagcaatcaa aagagaaaaa ctatcttcaa cagcgatgaa tgattttgct
     1681 atgccgcata tcgaagtaga ttttataaaa aagccagtaa tctgtgtcta cgttaatcca
     1741 gaaggaattg attgggatgg gacacttgtt cattctgttt tcttttttgc acttaatgaa
     1801 aaagtaaaac ctgaattgaa taaaatttat gaaacattca atgaaatcgc ttcgaatcat
     1861 gagttattag caaatttaaa aacgag
//
LOCUS       DACCXW010000229         1859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCXW010000229 DACCXW010000000
VERSION     DACCXW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.239"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="IVY71_002914"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="IVY71_002914"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7986899.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DACCXW010000230         1847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCXW010000230 DACCXW010000000
VERSION     DACCXW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.240"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="IVY71_002915"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="IVY71_002915"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7986900.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DACCXW010000231         1816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCXW010000231 DACCXW010000000
VERSION     DACCXW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.242"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            67..1632
                     /gene="guaA"
                     /locus_tag="IVY71_002916"
     CDS             67..1632
                     /gene="guaA"
                     /locus_tag="IVY71_002916"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7986901.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          558 a          377 c          385 g          496 t
ORIGIN      
        1 tttgaaaact aaaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat
       61 tcaaatttgt cttcaccaac acgatttgac gaagagccaa aaattatcgt acttgattac
      121 ggtagtcagt ataaccaact gatcactcgc cgtattcgtg agttcggtgt tttctcagag
      181 ttgatgagcc accgcattac cgcagaagaa gtaaaggcaa tgaaccctaa aggaatcatc
      241 ctttctggcg gaccaaacag tgtttatgat gagaaggcct ttggtattga tgaaaagatt
      301 tttgatttgg ggatcccagt tttaggaatc tgttacggta tgcagttgat tacttataaa
      361 ttaggcggaa aagttaaacc agccaaaaac cgtgaatacg gaaaagcaga attggaaatc
      421 acttctgcag aagcggccct tttccaaaac actcctgaaa aacaaaccgt ttggatgagt
      481 catggtgacc tagttaccca agcaccgact gatttcgatg tcgttgctac cagcaaagat
      541 tgccccatcg catcgatcca aaatgcagaa cgcaaaatgt acggaatcca attccatgca
      601 gaagttcgcc attccgaata tggaaatgac ttgttgcgcc attttacact agatgtctgc
      661 caatgtactg gtgactggac aatggataac ttcatcgaca tggagatcgc aaaaattcgt
      721 gaacaagtcg gtgacaaaaa agttttatta ggcctttcag gcggagtaga ttcctctgtt
      781 gttggtgttc ttttacaaaa agcgatcggc gatcaattaa catgtatctt cgttgatcac
      841 gtattgttgc gtaaaggtga agcagaacaa gttatggaaa gtcttggcgg aaaatttggc
      901 ttgaacatta taaaagtcga tgccaaagaa cgtttcttat caaaacttgc tggcgtgtct
      961 gatccagaag aaaaacgtaa aatcattgga aatgaatttg tatatgtctt cgatgatgaa
     1021 gcaaccaaac ttgccggtga agaaggcgtt tctttccttg cacaaggaac cctttatacc
     1081 gatgtgatcg aatcaggaac tgaaactgcc caaaccatca aatcacatca caatgtaggt
     1141 ggattacctg aagacatgca attcaagctg atcgagccat tgaacacatt gttcaaagac
     1201 gaagtacgtg aattaggtac ccaattaggc atgcctgaca gcatcgtttg gcgccaacca
     1261 ttccctggac caggcttagg tattcgtgtc cttggtgaat tgacagaaga aaaattagaa
     1321 atcgtccgtg aatcagacgc tatcttgcgc gaagaaatcg ccaatgccgg tttagatcgc
     1381 gacatctggc aatacttcac cgtcctacca ggcattcgct ctgttggggt tatgggagat
     1441 ggccgtacgt atgactacac tgtaggtatc cgtgctgtca cttctattga cggcatgacc
     1501 gctgactttg cccgtatccc ttgggaagtc ttgcaaaaaa tcagtgtccg tatcgtaaat
     1561 gaagtcgcac acgttaaccg tatcgtgtat gatattacca gcaaaccacc tgcaacagtg
     1621 gagtgggaat aaagagccaa cataatatgg atgaggaata cctataaatc aacattattc
     1681 aattactaaa gttcctgatt ttcgtaaaaa ataagatggt ttcctaccat tttccctacc
     1741 aaagcccttg ttagattaac tactaacaag ggctttgctt ttattcacta cctttatttt
     1801 cctattgaat tagctt
//
LOCUS       DACCXW010000232         1808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCXW010000232 DACCXW010000000
VERSION     DACCXW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.243"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..110)
                     /locus_tag="IVY71_002917"
     CDS             complement(<1..110)
                     /locus_tag="IVY71_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7986902.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
     gene            complement(206..541)
                     /locus_tag="IVY71_002918"
                     /pseudo
     CDS             complement(206..541)
                     /locus_tag="IVY71_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(665..1808)
                     /locus_tag="IVY71_002919"
                     /pseudo
     CDS             complement(665..1808)
                     /locus_tag="IVY71_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          480 a          368 c          335 g          625 t
ORIGIN      
        1 gttagttcat attctcgaat gatttctaca cgtggctttc cagctaagta aagattgacg
       61 atttgttgct tgaattcttg tgaaaaagtt cttcgtgttc tcttagacat aaaaattcct
      121 cctggtatgt tttcttctag tctacacacc ttaatttttc tgtctagtta attgtagcct
      181 atccactagt taattgtagc ctatccatac cttttcggaa gaggtgaatg acggtctcct
      241 ctaaggtatc attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc
      301 ggtcgcgcgg aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg
      361 agccgttacg agaattaccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga
      421 aagccgttaa ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata
      481 attcattcaa atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca
      541 tatggaagtc ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc
      601 cttttttcta tttacacaaa atattttaca ctctcgaaaa gtattgcatt ttattatata
      661 aaattcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg aacagctttt
      721 tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct ttccatagcc
      781 aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc gtcgcgactt
      841 tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca tccgcaaata
      901 ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct gttaggcgaa
      961 gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatgact cgttgatgat
     1021 ttttactttt ggtacattgg tttagcagag gacagttcac acattctttt ggattggatt
     1081 tgtattctcg gtacccttct cgagtaaccg ttgaaaaagc gagcatttga tcttgcggac
     1141 aaatataaca atcaaaatac tcgtcataga gaaactcatt ttttgataat agcccctttt
     1201 tccctttggg ccttgaataa ggcaaacaag gtcgaaggtt caaatgagtc aaaaagtgaa
     1261 caaacttagg tgtcttatac ccagcatcag cgacaacaca attgatttca ggaaaaagtc
     1321 tcttcgattg tttgacgagc tgaaaggcga cttaactgtc atgaatattt ccaggagtga
     1381 tgattgtgct aagaataaat ccattgtcgt cgcatgaagt atgggcagaa taagcaaatt
     1441 gtttttcttg ttcgcctttg acgtagtagc cgctttccgg atcagcttta cttatttttc
     1501 tttctttcag cttcacttcc ttttctaagt ccaaagtaaa agggcgtttt ccagcctcta
     1561 ttcgttgacg attgatctca tcttctaatt catcctgata ggctttcgct tcatcatggg
     1621 tcatttcttt cgtaaactta tgtttattgg cgttagcttt gatgtgggta gaatctagat
     1681 gaaaattatc ctctgttaca aaacctgctt taactgcttg ttctaaaata taggcaaaaa
     1741 tatcctcaaa caccgtcgtt tctctaaatc ggcgaacata attctttcca aaagttgaga
     1801 aatggggg
//
LOCUS       DACCXW010000233         1805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCXW010000233 DACCXW010000000
VERSION     DACCXW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.244"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            72..145
                     /locus_tag="IVY71_002920"
     tRNA            72..145
                     /locus_tag="IVY71_002920"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:106..108,aa:Ile,seq:cat)
     gene            691..1257
                     /locus_tag="IVY71_002921"
                     /pseudo
     CDS             691..1257
                     /locus_tag="IVY71_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            1461..1805
                     /locus_tag="IVY71_002922"
                     /pseudo
     CDS             1461..1805
                     /locus_tag="IVY71_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          635 a          316 c          331 g          523 t
ORIGIN      
        1 caaagaatga aacccattat tatctagtct atgtccattc tttttgtttg cagtagttgt
       61 gatggctttc cgtactctta gctcagttgg ttagagcaga cggctcataa ccgtccggtc
      121 gtaggctcga gtcctacagg gtacatactc taagtgattt aaattgcgaa taaaatataa
      181 aaatgactcc ccttttgact cccccaagca gtatatggat gtctctaaaa gttgtttttt
      241 tagataataa aaagctgata ataagcgaaa aacacttaaa atcagcttta aaccctattc
      301 tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta ccttccttat
      361 atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa cggccaaaca
      421 aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg caagcttttg
      481 aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc ccttattgtt
      541 gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta taaatgctag
      601 taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa gataaaagtg
      661 taaaaacgat ttgttttagg aggggaaacg atgtgtaccg caatcactta tgcaacgaaa
      721 gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt ggtcactatt
      781 actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa acattatgca
      841 atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc tacaaatgaa
      901 aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa agaattacaa
      961 gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg gcaatgctca
     1021 accatagacg aagctaaaga attattggct actatcaatc tagtcaatat cgattttagt
     1081 gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa atcgatagta
     1141 attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt gttgacaaat
     1201 aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct atcaaatgga
     1261 acgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aagtcccttc
     1321 tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta tgaatgattt
     1381 tactactgaa tcggctacac tagactagac agaaaaaata aggtgtgtag aataataaaa
     1441 aacacactgg aggatttttc atgccaagac gtcaacgaag aacctattca aaagaattca
     1501 aacaacaaat cgtcgatctc tatctcgctg gtaagcctcg cgcagaaatt attcgagagt
     1561 atgagcttac gccttcttct ttcgataaat ggatgaagca agcacaatca acgggctcat
     1621 tcaaagaaag agacaactta acaccagaac aagcagaatt gatcgcacta agaaagaaaa
     1681 ataagcaact cgaaatggag aatgatattt taaagcaagc ggcgctgata ttcggacgaa
     1741 aagacaagta attgatgcca acaagcataa atattccata tcagcgatgt gcaaaattct
     1801 aaata
//
LOCUS       DACCXW010000234         1798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCXW010000234 DACCXW010000000
VERSION     DACCXW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.245"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1031)
                     /locus_tag="IVY71_002923"
     CDS             complement(<1..1031)
                     /locus_tag="IVY71_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7986903.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1742)
                     /locus_tag="IVY71_002924"
     CDS             complement(1113..1742)
                     /locus_tag="IVY71_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7986904.1"
                     /translation="MGYRLYLEYQTDIQLFLNPRASKAELLAHIRSHGTFASILLIVL
                     TAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNALGNITAIFLMHRLKFLDKKTE
                     TNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADAMKLPLQQIILSIVIGVIPSS
                     VLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKDRKKKQGKQKHK"
BASE COUNT          598 a          407 c          320 g          473 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttt
//
LOCUS       DACCXW010000235         1773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCXW010000235 DACCXW010000000
VERSION     DACCXW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.246"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            81..1604
                     /locus_tag="IVY71_002925"
     CDS             81..1604
                     /locus_tag="IVY71_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7986905.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          641 a          285 c          359 g          488 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cggttctgtt gca
//
LOCUS       DACCXW010000236         1677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCXW010000236 DACCXW010000000
VERSION     DACCXW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.247"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..372)
                     /locus_tag="IVY71_002926"
     CDS             complement(<1..372)
                     /locus_tag="IVY71_002926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986906.1"
                     /translation="MNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLF
                     PHEDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEI
                     QYEGIGFTLIDLKNSTFEIKEG"
     gene            complement(647..1677)
                     /locus_tag="IVY71_002927"
                     /pseudo
     CDS             complement(647..1677)
                     /locus_tag="IVY71_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          480 a          315 c          271 g          611 t
ORIGIN      
        1 tccttctttt atttcaaaag ttgaattttt taaatctata agagtaaaac caattccttc
       61 atactgaatt tctgaaaatc gaatttttct cccagttgat agatcaaggg gcagaaaacc
      121 ttttattaca ttgtgagtgt caccatcatt tacaatgtca ttaggaattt tgattttaga
      181 attctgtgga gcacctattg gaagataata attcattata attttgtagt cttcatgagg
      241 aaataaagta cttttaggat tgctaagata ttctcgtacc gaatctaaga atggcttatc
      301 cattgaatct tcatccccat ttaaactaaa aaacattcct acaattgctt ttgcaactct
      361 aagtgaattc attttcttta ttgaatgtgt taaatcagac ttaccattct ttaaacttgt
      421 attgttataa aggaaatttt tattgtcttc tgcaaatcta atatattcct caacatactc
      481 actaccaaaa aaactattac atttactaca taacgtattg gaatggcaac agtttttttg
      541 acaaatttta taaggtgcag aacttctttc cgtatgctat tccgattgtc cttgacaatg
      601 agcctcctcg gatgtgacga tcttcggcag gagctaacag ttaaagttag actgcctcgc
      661 tagcgttagc acatccgagc tcattatcaa ggattcgctg cgccaatttc tggttacggt
      721 aaccaaccgg tgtctcgtag ccaagtgtac cgtgcaaccg aaggtggttc caccaattga
      781 catagtcaaa taactccaaa tccaattgtt gtaaggtttc aaatgtgtat tgatagacaa
      841 attctacttt caacgactta taagttgatt cagctacggc attatcaaaa ggacagcctt
      901 tatgactcaa tgatcgattg atgtcaaaag ttgttaataa ttcatcaata gcttggttat
      961 caaactcttt tccacgatca gtatgaaaaa tctcaacctc tgtcagaggt tgtttgatac
     1021 ggctaaatgc tttttttact agaacggcat ctttatgttc tccacaagaa tagccgagaa
     1081 tttctcgatt gaacagatcc aaaatgaaac agacataatt ccattttttc ccgactcgta
     1141 cataagtcaa gtctgttacg atcgcttcta atgggttgtc tcttaagaat ttacgattca
     1201 atacgtttgt cgttttggct tcattgcaag tagaatgatg tactttaaaa taagcaacag
     1261 tatagctcga ttttaatcct ctatttttca tgattctact aatttttcgt cggctgatct
     1321 gaatgcctcg ttttgataag gcttttttta tttttcttga gccgtaggcc tttcggctgc
     1381 ggataaattc ttcagcgact acttcttcaa gttctgattc gtctttcttt ggttttgatt
     1441 gataataata ggactgacgt gatagaccta atattctgca catcgctgat atagggtaaa
     1501 gatgcttatt cgcatcgatt acttgtctct tcgtccgaat atcagcgctg cttgctttaa
     1561 aatatcattt tccatttcta attgctggtt tcttttacgt agttctaaca attctttttg
     1621 ttcaggcgta agattatctt tttctttgaa tgaaccactc gttttagatt gctttac
//
LOCUS       DACCXW010000237         1617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCXW010000237 DACCXW010000000
VERSION     DACCXW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.248"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            22..1509
                     /locus_tag="IVY71_002928"
     CDS             22..1509
                     /locus_tag="IVY71_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986907.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
BASE COUNT          504 a          261 c          293 g          559 t
ORIGIN      
        1 aaaggagtat ataattaagt aatgcaagta aagaaaaaaa taatattgtc cattctccta
       61 gcaattattg ctggacttta ttcaatcaat tattttagaa atgttcatac tatttctaca
      121 tcgggggata ttgccttcca ttttgctaga gtcaaagggt tatcttcaat tttttctggt
      181 ccaatcaatt ttacgacgtt taatcattat gggagcggag tgaattattt ttatccctac
      241 ttgacctttt ttccggcagt cattttttat tggataagta ataatttaat cgttagttat
      301 atcttatatg tttggctatt gaatgtttgt actatcatgc tgatgtttca ttatggacta
      361 aaatttttaa aaagaatcga tgcggctttt atatttagct gtttatatac attttatggg
      421 taccgtacga ttgatattta tcatcgttct gctattgctg aagcgattgc acttacggtt
      481 ataccgatag ttatgtatta tgcttacgca ctgatttatg agaaaaagcc atgtgcaatt
      541 ccattagcag tcagtatgag cttactcatt tatacacatg ttctttccac ttttatgagt
      601 gtaatagccg ttggcttact agtcttaggc ggctttgtag taagtgaaac aagaaaaaaa
      661 gaaagcatcc ggttgattgt ggagttgtca aaggcagtag gaatgacgat tcttttaaca
      721 gcatactttt ggtatccgat gttccgacaa atcatggttc aatcgattaa tgagccagat
      781 aaaacgacct tacaacacca agcattaaat gtctcggata gtttgattgg tgcgatgaat
      841 aatgacctga caacctttac ggtaggaatc attggtatta tttctttgat ctgtccactc
      901 ttctttttta aaggatttaa tcgcaaagaa aaagtgattt accttagtgc ggtagtctct
      961 tggctcttat ccaccaaatt ttttccatgg tctttattac aaaatacacc cattcaaatg
     1021 attcaatttc cttggcgaat cttaggcttt caagtactgt ttggctcgtt gattttatcg
     1081 ttcgttttca tgaagagcac atcagctaag caaagaaaag tagttaatat aacggcaatt
     1141 gttttattac tgttggctgt atctgctgca acaaaagcaa actatgaaca taaagttgtc
     1201 actcagagag atcacttggt aatgaatcaa gaaaatctta ttcgttacac gacactcctt
     1261 cccggaggac tttatgacta tgcacccacg gaagcattac aatacaaaga acatttgcag
     1321 aatcatgaag taagaattgg caaggaatgg catactatgc cttatcaagt gatggattcg
     1381 aaaatagtct atacagtaca tgaatctaaa ggacaaaaaa taacgcttcc tgtatttgat
     1441 tattggggaa cagtcgcaaa agtaaatgga caaaaaagag agattgataa taggactagt
     1501 gtcgattgaa ggaacacaag gagaaacaat cattgaagtt tcttccattt atcgtccaac
     1561 tatgaagtta gcttttttaa ttagtttatg tagttacctc tttcttttaa ttagttt
//
LOCUS       DACCXW010000238         1611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCXW010000238 DACCXW010000000
VERSION     DACCXW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.249"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..377
                     /locus_tag="IVY71_002929"
     CDS             <1..377
                     /locus_tag="IVY71_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986908.1"
                     /translation="SVTGTTIPTFADSTNEPTTVVDNNQELTPLDRNGIAIGNPTNDL
                     TVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLY
                     LQSYKSAGNNWIATYSGTLTRK"
     gene            387..590
                     /locus_tag="IVY71_002930"
     CDS             387..590
                     /locus_tag="IVY71_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986909.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            801..1385
                     /locus_tag="IVY71_002931"
     CDS             801..1385
                     /locus_tag="IVY71_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7986910.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          568 a          236 c          296 g          511 t
ORIGIN      
        1 ttagtgtaac tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg
       61 tagtagataa taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc
      121 caactaacga cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag
      181 cagcttctaa aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt
      241 ctagacctcc tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa
      301 aattgtattt acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa
      361 ccttaactag aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc
      421 aagatagcga tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc
      481 agcgaacttt acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg
      541 aagaaaaaag aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa
      601 aaaagagtgt ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat
      661 ttctggacac tttctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata
      721 aacccataac aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa
      781 ttaatgaagg agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa
      841 atttggattt acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc
      901 atatgagtgg ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct
      961 ctggcgatac acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga
     1021 tttcaatcgt caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata
     1081 ttacgatgga caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg
     1141 cagaatttga gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg
     1201 caagaggacg gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga
     1261 agactttagt ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa
     1321 gaaccaccat ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc
     1381 attagttttt gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag
     1441 tatgagaatt ttttgtaagt gtctactgat aacttattct caaatgttgt aaggatttta
     1501 ttcatttgtt aatattaaag aacggaactc caaaaaagtc cgtactctaa tttgcagtct
     1561 ctcactcttt tagagtgaga gtttttatta gattttcaaa ttaattagta a
//
LOCUS       DACCXW010000239         1541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCXW010000239 DACCXW010000000
VERSION     DACCXW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.250"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(58..1493)
                     /locus_tag="IVY71_002932"
                     /pseudo
     CDS             complement(58..1493)
                     /locus_tag="IVY71_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          497 a          261 c          258 g          525 t
ORIGIN      
        1 cactctcaat cttagctgtt gttctattca tcattactct tattgtaagt agttcagtca
       61 aatttgcttg ttcaattgat cgataacagt atgaattgtt tgaaaagtaa tcggatatat
      121 aattgttttc tcactaccta agccttcttg gtataaagaa ctatcgcaaa gtataatccg
      181 ctgagtacaa aagaaagtgt cgtctactat ttctctaagt tgtttacaaa tgatattact
      241 aatagaaaag tgttctccca aaatgtcttc taactgttgt cgatagtata aatacctaaa
      301 gtctgacgga gctactatga acacttcaat tggagatttc cgtttatgct catttaaaag
      361 acttaaacta atggttagtt tacgaataga attctcattc caacgcagaa ataaattgtt
      421 ttgttttcgc cattgttcaa gaatttcatg aacttctaag tacaaagcgc gttgctcttc
      481 atttaacaag taagtcttat caggttgaaa tatctgaata tcagcccaaa gtgagcggat
      541 aaacgggaga agtgctttat caaaaagtat ctgattttct tcagatatat tcagagataa
      601 gataagtttt tgactgagct caatcaccaa agggttatct gctacatggt tttgataaac
      661 aacgttcaag tctttttgta acaattcaaa gttatcgcat agataatttc tagagttgaa
      721 tagaaagtag atataaaggc attcatcttc gtcatgacat aaagagaaag aaagttgctc
      781 taatcctttc tgtattagct gaaacaaggg agtatgtcgg gcaactcttt tttcttcctg
      841 actaaaatat attgggttag tcttagatct ctgcaaaccg atagcgatac ctaataaaat
      901 aaattgtttt tcaactgtag ggtaacttct tgaaaaaaat gtttttctac ataatcaatc
      961 aattgttgac cgctttgaat aattgattta tttagctgga tcaactctgt ccgatttgtc
     1021 tcatagatta tcgttagtaa caaacttcga taatcctttt caggaatttc aaaatggcca
     1081 tcagcattta agtactgcat ttgtttaaaa aaattaatca actcatttcg taagttgtaa
     1141 actttgctta acgaaatgaa ggtagtatca gctatattac tccaattgtg ttcccctgat
     1201 aaataatgtc ctagtacatt gaaaaagtca gagcgctgat agatgagttg agtgagtgcc
     1261 aacctcgaaa acgaagagtc gaatgcgact gtaaagctac ttctagattt ctccactgaa
     1321 ataatttgct gatggagtat gtcggtaatg cattccaagt cgttagtgac agtgagtggg
     1381 caaacgttca attgatctgc tatttcgcga atctgaatgg tccctttttc gtgtaattgc
     1441 tcacaaatgt aaacttgacg aaaaatactt ttctccagat aggtttccaa catagtactc
     1501 ctcctagtca ttttatacat aactattata actctaccaa t
//
LOCUS       DACCXW010000240         1525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCXW010000240 DACCXW010000000
VERSION     DACCXW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.251"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            15..1481
                     /locus_tag="IVY71_002933"
     CDS             15..1481
                     /locus_tag="IVY71_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP7986911.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          324 g          495 t
ORIGIN      
        1 ttggggtata gattatggaa aaacagcaag acaagggctt cttcgggcag ccgaagggat
       61 tgtcaacttt gttcttcact gagatgtggg aacgatttag ttattacggt atgcgtgcat
      121 tacttatcta ttatatgtac gatacagttg ccaacggggg acttggatta ccacgagcaa
      181 cagcattagc tatcatgtcg atctacggat ctttgatcta catgtctagt atcgtcggcg
      241 gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt attcttcggt ggagttttca
      301 ttatgcttgg ccacatcgtc ttggctttac catttggtgt aagtacattg tttgtttcga
      361 tggcattgat tattatcgga accgcctttt tgaaaccaaa tgtttctggc atggtcggac
      421 atctttattc aaagactgac ttgcgaagag atgcaggatt ttcgattttt tacatgggtg
      481 tcaacttagg ggcattatta gcaccactaa tcgttggtac aatcggtcaa gaatataact
      541 atcatttagg gttctcttta gctgctattg ggatgttctt tggattatta caatacatca
      601 tccaaggacg caagacatta gacggagtgg gtacaacacc gccgaatcct ttgacaactg
      661 aagaacgtaa aggattcatc cgtaacttga tcatcgcact tgttgttatc gctgcagtat
      721 tcggcggggc acaactaact ggtcatctga caattgactt ttttgtcaat acaatcagtg
      781 tattggggat tatcttaccg ttgtattact ttatcaaaat gctgacttca aaagatgtaa
      841 ctgctgaaga aaaaccaaga gtatgggctt atatcccatt attccttgca gcaatcattt
      901 tctggtcatt acaagaacaa ggttcatcta tcttagcttt atttgctagt gaacgtacac
      961 aatctacgct atttggtgta acgatccaag caagctggta tcaatcattg aatccaattt
     1021 tcatctttat tctgacacca gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac
     1081 catctacagt agtgaaattc tctcttggac ttttatttgc aggattatcg tacttgctat
     1141 tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt aagtccattg tggttgttgg
     1201 gaagtttctt catcgtggtt attgctgaat tgtgtctttc accagttggt ttgtctgtga
     1261 caacaaaatt agcaccacga gcatttgaat cacaaacaat cgctatttgg ttcttagctg
     1321 atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat
     1381 ctgcttactt cggtattatc ggtgcagtag caatcgtcgg tggtatcttg ctatttactg
     1441 tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta atcaatgaac ataaaaacag
     1501 gaattgctta tttgcagcag ttcct
//
LOCUS       DACCXW010000241         1483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCXW010000241 DACCXW010000000
VERSION     DACCXW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.252"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            64..972
                     /locus_tag="IVY71_002934"
     CDS             64..972
                     /locus_tag="IVY71_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP7986912.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1483
                     /locus_tag="IVY71_002935"
     CDS             972..>1483
                     /locus_tag="IVY71_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986913.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          511 a          216 c          345 g          411 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DACCXW010000242         1480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCXW010000242 DACCXW010000000
VERSION     DACCXW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.253"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            64..972
                     /locus_tag="IVY71_002936"
     CDS             64..972
                     /locus_tag="IVY71_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP7986914.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="IVY71_002937"
     CDS             972..>1480
                     /locus_tag="IVY71_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986915.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DACCXW010000243         1461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCXW010000243 DACCXW010000000
VERSION     DACCXW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.254"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..983
                     /locus_tag="IVY71_002938"
     CDS             <1..983
                     /locus_tag="IVY71_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP7986916.1"
                     /translation="ISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIILS
                     LISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRK
                     SIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTP
                     LPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVD
                     LDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYA
                     FLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            985..>1461
                     /locus_tag="IVY71_002939"
     CDS             985..>1461
                     /locus_tag="IVY71_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986917.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDY"
BASE COUNT          498 a          174 c          240 g          549 t
ORIGIN      
        1 tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa ggttttattg
       61 aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt ggatatactt
      121 ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg attcgtgttt
      181 tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg tctagaggtg
      241 cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc aattcaaaat
      301 ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta agcatattat
      361 tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt ttagcaacaa
      421 ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca gataaaactc
      481 cacttccaga aaaagaagta atattacagc attctagtga taactccgaa atttcagctg
      541 gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg ttatttggtt
      601 atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa gtagatcttg
      661 atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat ggatttcttc
      721 aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc tatgtgttaa
      781 atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg tttcttggta
      841 ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct catgtacttt
      901 tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc atttcttact
      961 tgtatttaaa aaaggagaat taatatgaag acagctttca ttgcttggac tcctttacat
     1021 ttgattaata tattaaatgt ttatcaaaat ttttaccgag aatcaaaagg agatttattt
     1081 atttattctg aatttgaaaa cgctagaaga tatagtgagc gtataaaaga attagggcta
     1141 tttaatgatg ttgtttttgt gaatcaccgg aaaatcggtt cttcctttaa acgtaaaatt
     1201 aacttgttaa ctaaccataa tcaatttttc tcggatgatt tatccgttta tgatgaaatt
     1261 tttatacagg gggaaaatta tttcacaaaa atacttttct ccaatgttta caaaaaaaat
     1321 ccagatgtaa aactaaatta tatagaagat ggtttgggta cttatttgaa gaagagtata
     1381 ttttcaacaa gtaaaaacgt tcaaagagtt tttaaaatat taaataaaaa tagcatcttc
     1441 aaaaaaaaaa ttgacgatta t
//
LOCUS       DACCXW010000244         1455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCXW010000244 DACCXW010000000
VERSION     DACCXW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.255"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            184..864
                     /locus_tag="IVY71_002940"
                     /pseudo
     CDS             184..864
                     /locus_tag="IVY71_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            926..>1455
                     /locus_tag="IVY71_002941"
     CDS             926..>1455
                     /locus_tag="IVY71_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986918.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTM"
BASE COUNT          520 a          228 c          303 g          404 t
ORIGIN      
        1 agaaaggtga ttccatgttt aataaatggg caaaccaccc aatgaactcg agaggtagag
       61 ataatttgaa ggagacggac aagttctggg aagaagtata tagtaaaaac ggttctgttg
      121 caaagttttc caaaaaatct attttagtgt aaaattgaga aaaaagacag agaggacaga
      181 gtaatgaatc attttaaagg caaacaattc aaaaaagacg tcattattgt cgctgttggt
      241 tactacctgc gttacaatct aagctatcgt gaagttcagg aattgttata tgatcgtgga
      301 ataaatgttt gtcatactac gatttatcgt tgggtataag agtacagcaa agtcctctat
      361 tatctttgga agaagaaaaa tagacaatcc ttctattcat ggaaaatgga cgaaacctat
      421 atcaaaatta agggacgttg gcattatctt tatcgtgcaa ttgatgcgga cggcttaacc
      481 ttagatatct ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga
      541 ctccataaac agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc
      601 tccgccttta gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg
      661 aagtatctta acaatttaat agaacaagac catcgaccta ttaaacgacg aaataaattt
      721 tatcaaagtc tccgtacagc ctcttccacg attaagggca tggagaccat tcgaggaata
      781 tataaaaaga accgaagaaa tggaacgctc ttcggctttt cggtgtctac tgaaatcaag
      841 gtattaatgg gaataacagc ctaagatatt tggagttcag agagggcgcg tttgattttc
      901 aaacttcgca acagaaccga gaaccttggg ttttcaagtg ctttttggtt cattaatctt
      961 aaccatagtg tttttaaagt ggaaaacaag caacaaaaaa agtatgtgga gccttgtagg
     1021 aatagttctt attattttta ctgtgactgc agcaacagaa gcaaattatt cgcataaaat
     1081 tcagtcgtat aaaggtcgtt tagttatgac caaaaaagat gttacacgtt atacatcctc
     1141 aagaactgga ggcctatatg actatgcacc attagatgca ttgaaatata aagatcattt
     1201 aaagaaacat gaagtaaaag ttggtgatcg gtgggagaat gcatctcata aagcatataa
     1261 ttccaaaata gtctatacag tagaagatgc cgatgagcaa aatatcacat tacctgtatt
     1321 caattattgg ggaacagttg tgaaagtaaa tgggaaaaag agcactatag aaaatgacaa
     1381 tggtttagtg tctattaaag gtacggggaa aagaactata gttgaggttt catctacgta
     1441 tccaactacg atgat
//
LOCUS       DACCXW010000245         1395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCXW010000245 DACCXW010000000
VERSION     DACCXW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.256"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            10..420
                     /locus_tag="IVY71_002942"
     CDS             10..420
                     /locus_tag="IVY71_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7986919.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            434..790
                     /locus_tag="IVY71_002943"
     CDS             434..790
                     /locus_tag="IVY71_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7986920.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          511 a          208 c          261 g          415 t
ORIGIN      
        1 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
       61 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
      121 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
      181 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
      241 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
      301 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
      361 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
      421 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
      481 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
      541 cgatttaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
      601 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
      661 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
      721 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
      781 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
      841 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
      901 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
      961 tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc
     1021 aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga
     1081 taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat cgaaaaaaac
     1141 ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg ataaatgagt tgctgtaaga
     1201 tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt gacaattact tgtctctctg
     1261 atatgatata aaagttgcta cggcagccaa aaacgaatcg gggaacactc ctgatgaagt
     1321 agacctttga aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc ttgattcaat
     1381 tcaagacaac aaaca
//
LOCUS       DACCXW010000246         1395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCXW010000246 DACCXW010000000
VERSION     DACCXW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.257"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            104..718
                     /locus_tag="IVY71_002944"
     CDS             104..718
                     /locus_tag="IVY71_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7986921.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(742..1092)
                     /locus_tag="IVY71_002945"
     CDS             complement(742..1092)
                     /locus_tag="IVY71_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7986922.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1086..1349)
                     /locus_tag="IVY71_002946"
     CDS             complement(1086..1349)
                     /locus_tag="IVY71_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP7986923.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          444 a          262 c          228 g          461 t
ORIGIN      
        1 taaggtatac cctaactaga cacgaccgcg acattgaaaa atctggtttt ctcgtgtatg
       61 attagggtgt tttttaaata gttgcagaaa gtaggtaaaa attttgagag ttgcatacat
      121 acgtgtctca tccatcgatc aaaatgaaca acggcagatt gaagaaatga aaaaattcgg
      181 tgcagagaga attttcattg agaaacaatc tggcgcaacg attactcatc gacctgtttt
      241 tcaagaagca ctggatttcg tccgagatca agatattttt attgtggaag ctattgatcg
      301 acttggaaga aattacgacg agatcatcgc ttcagtcaat tacttgaaga agaaaaatgt
      361 gcagctgatc atcacaagtc taccgatcat ggccgaagca attggcaatc ctctcctgga
      421 taaattcatt aaagatttga tcattcaaat tttggccatg atcgctgaac aagaacgtac
      481 cgaatctaaa cgacgtcagg cacaaggcat taagattgca aaagcgaacg gtgtttataa
      541 aggacgtcct aagctataca gtgccaatgc aaaagatccc caacgtcgtc ttgtttataa
      601 aaacatcgtt gaggatctaa aaaaaggtgt tgcgatcgct aagattgcaa aagactacaa
      661 tgttacgaga caaaccgtat atagaataaa aaaagacagc atggtcaatc acgaatagtt
      721 gatcatgctg ctgtttctat tttatttgct tactttttga aaaactgttc taactttcat
      781 caataactcc tcaactaaac tatcatgcgc tttttccaaa aaaatacact ctctggcttc
      841 ataatcaatt gtcgttagtt gatctaataa tacttttcct gttgtcttca tttcatattc
      901 tgagtttata tttacatata gcggatagtt tctagttgta ctagaaattg gagcaactgt
      961 aacaacgtta ctataatccg caataatatc atgacttaaa acaatataag gtcttttacc
     1021 tgtctgttcg tgtccagatc gaggtgctgt attcaataat agaatatcgc cttgtctagg
     1081 ttttctcacc atttttcatt acctactgct tcacctaaat tttgaatcgt cacatgattt
     1141 ctttcatcag tgtaattttc aaataactgt tcaattgtta attcattttc tatattagcc
     1201 gcttcgataa ttagccgctt ctgaccgttt acttcttctt gtattatctt cactttgtct
     1261 tctgtatgaa ttccaagata ttccataata tactttggca atctaaggcc attactattt
     1321 ccccattttt ttataggtaa catttccata gtagacacct ctttctgtat atacatagta
     1381 taacatacat aagaa
//
LOCUS       DACCXW010000247         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCXW010000247 DACCXW010000000
VERSION     DACCXW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.258"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1393
                     /locus_tag="IVY71_002947"
     rRNA            <1..>1393
                     /locus_tag="IVY71_002947"
                     /product="23S ribosomal RNA"
BASE COUNT          374 a          300 c          424 g          295 t
ORIGIN      
        1 aagtctgaag aggagtcaaa tgcttctttt cttaaggaca agctgtgatg gggagggaaa
       61 taatagtacc gaagttcctg atgtcacact gccgagaaaa gcttctagtg agaaaacagc
      121 ggcccgtacc gcaaaccgac acaggtagtc gaggagagaa tcctaaggtg agcgagagaa
      181 ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgatcat
      241 acgatcagcc gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcaa
      301 aatcgtaaga tgaagtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg
      361 cttagcgcaa gcgaaggtac gaattgaagc cccagtaaac ggcggccgta actataacgg
      421 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaacgat
      481 ttgggcactg tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta
      541 cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt gagtgtctgt
      601 accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc gatggaggcg
      661 ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa tcgtggtggg
      721 agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg
      781 cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc agaagggagc
      841 ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt
      901 ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct
      961 cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct
     1021 ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg
     1081 gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga aatttgagag
     1141 gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc agttgttctg
     1201 ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc atctaagtgt
     1261 gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc ctgagagatg
     1321 atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact gatactaatc
     1381 gatcgaggac tta
//
LOCUS       DACCXW010000248         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCXW010000248 DACCXW010000000
VERSION     DACCXW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.259"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            11..1036
                     /locus_tag="IVY71_002948"
     CDS             11..1036
                     /locus_tag="IVY71_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7986924.1"
                     /translation="MVPCYNVEKYIDECINSLINQTLKDIEIILLNDGSTDNTLAILK
                     KYEKLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFESLYNCAIA
                     ENADIAIGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNRLYSHNAW
                     DKIYRRSLVLEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYRQQNNSIM
                     HKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQKNVGGTCE
                     DYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKMYKTAYNM
                     LWRIYNR"
     gene            1050..>1393
                     /locus_tag="IVY71_002949"
     CDS             1050..>1393
                     /locus_tag="IVY71_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7986925.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSER"
BASE COUNT          546 a          133 c          263 g          451 t
ORIGIN      
        1 gattagtgta attgtacctt gttataatgt tgaaaaatat attgatgagt gtataaatag
       61 tttgataaat caaacattga aagatattga gattattttg ttaaacgatg gttcaacaga
      121 taatacgttg gcgatattaa agaaatatga aaagcttgat tctagaataa aagtttacga
      181 acataaaaat atggggttag ggccaactag aaatcgaggg atcaaaatag ctaatggaga
      241 atttttggct tttgtggatt ctgatgattt tgtttccaat actatgtttg aaagtttata
      301 taattgtgca attgcagaga atgcggacat tgcaattgga gaagtaggca ttttttctga
      361 tagagaaata gaaagagtaa aaataagaaa aagtctaacg aacttaaaaa caattgaatt
      421 agcaacatat aacaagaaag aattttttaa gcaattctat ttcaatcgtt tatattcaca
      481 taatgcttgg gataagatat atagacgtag tttagtatta gagtttaatt tatcatttgg
      541 tgataacaaa ataatttttg cagaagataa ttggtttcaa ttacaagtat ttatgacaaa
      601 tacaagaatc tgtttcgtag ataaattgtg ttattattat cgccaacaaa ataattctat
      661 aatgcataag ccaaaaagta atttggtttt tagacatgga aatatgattg ctgattatga
      721 aaagttgata aggcaaaatg gaaacaacat aatagataaa aaagcttgta tgttggttgc
      781 atttgatgta ctaattatgg agatttttaa tcaaaaaaac gttggtggaa catgtgaaga
      841 ttataagaat gcattagttt ctttgaaaca tcataaaata ttgagagaaa atatttcaaa
      901 tatagttaga atgaaagcat atgaacttga acctaatatg agaaagaagt tagcccttgt
      961 gaccattagt ataatgttta attttaaaat gtataaaaca gcgtataata tgctctggag
     1021 aatatataat cgataaagag gtaggaaata tgaggaaatt gacctttgtg ataggaatta
     1081 tgggaaaagg aggggcagaa cgagtaattg cgaatttggc aaataatttt attcaagata
     1141 actgggaaat tgatattata actatatatg ggaatcggca agactatgaa ctgaatagaa
     1201 aaatacaggt acatccaata atatgtaaaa gttcgtttcg tgcatttaga cctttggaac
     1261 gctttaagga acttaggaaa aaaataaggg aaatacagcc tgatctggtt atttcgttcc
     1321 ttgctgatgt aaatatccat gtcattattt cattgttagg agtaaaacaa aaattaattg
     1381 tttctgaacg aaa
//
LOCUS       DACCXW010000249         1357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCXW010000249 DACCXW010000000
VERSION     DACCXW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.260"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(328..942)
                     /locus_tag="IVY71_002950"
     CDS             complement(328..942)
                     /locus_tag="IVY71_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7986926.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          438 a          267 c          228 g          424 t
ORIGIN      
        1 agttttgggg aagactcttt catcaacgtg tattaagaga caagaaagaa tccatatgga
       61 ttctttcttg tctctttttc cactactttt tatttttctg acactacata ttcttataac
      121 tcatttctca taactgtgaa acaacttata aaaataacaa actttaaaca aaacaagctt
      181 aaaatcaaag atgatccatc ttaaaataac taaaacaaaa aaagtagtcg ttgttaatcc
      241 tcattaataa tcgttttctt tttttcaaac gctccatttt tatgtggaac attatttttt
      301 taaggaactt tgtcaacatg tggataacta ttttccactt gtgtcctgga ctagctgttt
      361 tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg ctgttccgca
      421 gatcgctgga tctagatttg gcaaagtcaa gactgcatca tgtaaattgg cgtggttgtc
      481 caaaaccaaa tcagcaaatt caaataatcg caaaccactg ctatgttgcg atttgatact
      541 tcgtgacaaa tcaaatccag taacaatgat taatggatga ccattttctt tgatccactg
      601 agctagttcg atgattgccg ggtttcttcc ttcattcgaa agaagaagaa aaatgtcttc
      661 tgatttgaca gaaagctgat caataattgc ctgtcctgca ccttctagcg tttcatagac
      721 acctccggct ggattttcaa tcaatatact tggtacaaga tccggggttt gatcccaaaa
      781 ttcaaatgct acaccactta gtgtgcggct agcatatatc tgcaatctac caccttgatt
      841 aatttgtttc gcgatccaag tgccagcttg aagcatcagc ggctcttcta tccttaattt
      901 ctcttccaat tggtgataat agtcaaaata acataaactc atttatctct cccgcctagc
      961 tatacttgac atgaatagaa acagctgcct aatctaagga agtacataaa aaaacagtag
     1021 ttagacagta acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat tgaacgaata
     1081 cgcccaatac cttgacatag caaataaaac aacgtgcaga ccatggaaaa tatctacata
     1141 ggggtagaat tttcacccta gattaggtac aataaattag gtcgatgcac tgcattgact
     1201 atggcaaaat gatcggtcag ggtgtatgtg tgtgactggt cattccctga ttcttagcgc
     1261 caactaaggg tcagggagcc tttttttatt cagctgtttc tatctgaatt tattgtaagt
     1321 aatctcttat ccaagtgcaa gcaagaaaat acaaaaa
//
LOCUS       DACCXW010000250         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCXW010000250 DACCXW010000000
VERSION     DACCXW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.261"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..166
                     /locus_tag="IVY71_002951"
     CDS             <1..166
                     /locus_tag="IVY71_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP7986927.1"
                     /translation="NYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSEL
                     QDIYNYLYRR"
     gene            171..1118
                     /locus_tag="IVY71_002952"
     CDS             171..1118
                     /locus_tag="IVY71_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7986928.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          442 a          188 c          287 g          438 t
ORIGIN      
        1 taattatatt tttgatgggc aatgtgggat aataaaaatg agagattatc taattttaga
       61 tattgatagt gaagaagatt ttaaatggtt agaatttttg tttcctaaat ttttagaaga
      121 ttcttcggag cttcaagata tctataatta tttatatcgg aggtaaaagg atgagctgtt
      181 atcttatcac cggtggggct ggcttcattg gttctacctt agccaatcat ttaggagaag
      241 aacatgtggt aatcgtcgtc gatgacttat caatgggaaa agtagaaaat cttgatatga
      301 cgaaaaatat cacttttatt gagggagatg tcgctgagaa atccttgatg gaagagatca
      361 tgaaagctaa taaatttgat tatatctttc atttagcagc agttgctagt gtggcagatt
      421 cagttgcccg accagtagaa acacatcgtg tgaattttga aagtgtatta atgattttag
      481 aattgatccg caagtatcaa cctgatttaa aacggatcgt gtttagttct tcagcggctg
      541 tttatgggga tgaaccgact ttaccaaaaa aagaagagtc cgtgattcgc ccattgacgc
      601 cttatgcggt tgataaattc gctgcagaac aatatgtgct agattattgt catttatatg
      661 atgtacccgg tagcgcagtt cgttttttca atgtctatgg tccaaatcaa aatccgaatt
      721 ctccatacag tggtgtgatt tctattctcg tagatcgcta taagaaacaa atcgtaggag
      781 aagcaacttc atttacatta tttggtgatg gaagccagtc tagagacttt gtttatatag
      841 atgatgtaat ccaagcatta cttttagttg cgaatgagga agctgcactg gggaaacaat
      901 tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt gattcatgca atcaatgaaa
      961 ttctcggtgt agaattgaca cttgaataca aagaagagcg ttcgggtgat atccatgact
     1021 ctctagcaga tattactaag atccagtcaa ttggctatca accgaaatat ggtattttaa
     1081 gtggaatgac taattatttg aaaactgaaa tcaattaaaa catactaaga taagattaga
     1141 gggtaaattc aggctctttg tcaataagga ctgatgagtt ttgtaaaatc aaatctggga
     1201 tagaagggct ctttgtcaaa tgatgttggt aaagagaaat cttagaataa tttggacaga
     1261 acagaaagaa gaaaacctct ttctgttctg tttatttggt taaaaatgtt ttgccatgac
     1321 gaatcataat tcgtagtttg aaattgtaaa agttg
//
LOCUS       DACCXW010000251         1299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCXW010000251 DACCXW010000000
VERSION     DACCXW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.262"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(849..>1299)
                     /locus_tag="IVY71_002953"
     CDS             complement(849..>1299)
                     /locus_tag="IVY71_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP7986929.1"
                     /translation="EFETIPEELKDKRVEEVDRTPAENLAYQVGWTNLILKWENDERN
                     GLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKELEEILNRNIDDINMMIDSMSDEDL
                     FTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTFRTKIRKWKRLAL"
BASE COUNT          402 a          257 c          196 g          444 t
ORIGIN      
        1 taaattgatg gtatttagat aacacgaaag aataaaaata acagctgata gtaaagtttg
       61 ttgacgattg ctactcaatc gaaagtgagt tagtgcgtat gaacttgctt ctgaaaactt
      121 atgaaatgaa agacctttta catcttgcag aaatcgttac tgggattaaa ctaagcaata
      181 aaaactaatg ttcccctcat tctttgaact tctactattt gatttattta ttctgataaa
      241 tgacgatttc accttcagga tcttcaaatc ccgtatagaa tttatttaag atctcttcag
      301 tatcagtaat aggataggcg ataggccaca taccctttga tggttagaat aaactgctca
      361 aaatctgtat cgtatgttgg ctttagcata ttaacaaagg ctatctccaa attataaacc
      421 ataagcgttg aatcgttctt aacaatttca attcttccta acccaaagtc agaagatata
      481 attaccggac ttgccttacc cttcatgtgg gttgttgaga ttccatattt aaagggaatt
      541 gtcggtacta tcggcggtct gaaaaacatc ccaaaaataa aaaaatcttt cccctgctag
      601 tctcctaagt ccctttgtag cggatatggc accataatct cattttatcg aagtgagtca
      661 tggtttatct gttggccaac caaaattgat acaatctaac gatgcaccct ctacggcatt
      721 ctaggggagt gcacttgcat tcacccaata aaaaccggca gttagcaaag aacttatgta
      781 gtcgtatgtt cttactgtta acatacaaga ggtctccttt ccacaattgc ttagcagtaa
      841 acgggaattt acaaagctaa tctcttccat tttcttatct ttgttctaaa agtcccaaat
      901 ggagcaactg tatttacatg aataaattta tatacttccc atacagccgt tttggttgcc
      961 tcgtctgccc attttctttt gtgtgcagtg aatagatctt catcactcat tgaatcaatc
     1021 atcatgttaa tatcatcaat attacgattg agaatttcct ctaattcttt caatggtaga
     1081 tgtgcatagg tatttgtaaa ccacttatat aactctccta actgattcca tttaaattga
     1141 tcagaaggcg ttttaacaga taatccattt ctttcatcat tttcccattt tagaattaaa
     1201 ttcgtccaac caacttgata tgctaaattt tcagcaggtg ttctatccac ttcctcaact
     1261 cttttatcct tcaattcttc aggaatagtt tcaaattct
//
LOCUS       DACCXW010000252         1296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCXW010000252 DACCXW010000000
VERSION     DACCXW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.263"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(35..334)
                     /locus_tag="IVY71_002954"
     CDS             complement(35..334)
                     /locus_tag="IVY71_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986930.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(340..570)
                     /locus_tag="IVY71_002955"
     CDS             complement(340..570)
                     /locus_tag="IVY71_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986931.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(820..1020)
                     /locus_tag="IVY71_002956"
     CDS             complement(820..1020)
                     /locus_tag="IVY71_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7986932.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          445 a          216 c          196 g          439 t
ORIGIN      
        1 atccgttccg ggctaccttt ttaggtagcc tactttaatc tttgtatctt tctggatcaa
       61 cgaaagtata ctttatatag tcataacgcc gatgatcgct tcgagcgtct agcacatcag
      121 tcacgatatc aaacaaaaaa tatacgtctt tcttcattct agttttcgca gcagggattt
      181 taaagtagtt cttattagaa tagtagagat taattaataa gctatcttcg attgctaaaa
      241 agaaaacttc tgaatcccac accttataaa aatctttgac aaatctattc gaaggatcaa
      301 atttaaacca taattgcgtc tcattaaaaa gcataaccat tactccgatc tatttttaaa
      361 cttagtctct acctctaata tatatcgagt ttttattttg ccttcagaga atactgtttc
      421 tttttttgca gttacaggtt gtttattttc ggaaaaagct aatatagcta aaattgaaac
      481 atccatctgg aatttgtctt tttcgttgct ttgctcataa aaatctgcat attcatcact
      541 aatatttttt ctaataaatt cttccatcat aaagatcacc tcgaaaagag tatacgaaca
      601 aacgttcttt ttgtaaaggt ggaatttatt atactaaata aataggtgaa caagtacttg
      661 tgccaaatta tgtgccaaaa aaaatcgaat ttaataaaac acaaacaaaa agaaatccta
      721 ttatgataac atttcttata ataacaaaca caacagaaga cgtgtaatag ttagtcagga
      781 acgtacaaat aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat
      841 tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg
      901 ataaagtaaa atagatgcat gcgtatgtct tagtccatca ataaacagtc gaagagaaaa
      961 tagggaaaaa ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat
     1021 acttcaatag attcgaatta aacgatttcg attggcttat accagtccta tccaacgaaa
     1081 gatatatcgt tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat
     1141 gaactggaga tgacttttta ttatttttag taatagctga tgggttaata cttacaactt
     1201 tccttttcaa agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa
     1261 gccaatagct tagatccttt attctatgct ttatat
//
LOCUS       DACCXW010000253         1296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCXW010000253 DACCXW010000000
VERSION     DACCXW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.264"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..721)
                     /locus_tag="IVY71_002957"
     CDS             complement(<1..721)
                     /locus_tag="IVY71_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HAP7986933.1"
                     /translation="MFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAICTWHWRKGD
                     SIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSESDMIMVKWY
                     SCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIVVPIVFVSK
                     LLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKVITRFVFII
                     FALYSLLGIMSGDRGSWLYSSI"
     gene            complement(764..>1296)
                     /locus_tag="IVY71_002958"
     CDS             complement(764..>1296)
                     /locus_tag="IVY71_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7986934.1"
                     /translation="FITACRLDKQKNLPLMIDAIGKLIHEGYECELDIYGEGGLKTEL
                     ESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEALAIGVPVIAT
                     DCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQKGIKLRKELT
                     SKNISNIWYEYLNQFF"
BASE COUNT          456 a          254 c          145 g          441 t
ORIGIN      
        1 taatagagct atacaaccaa cttcctctgt ctcctgacat aattcctaat aaagaatata
       61 aagcaaaaat aataaaaaca aatctcgtta ttacttttga aaagttgcta ccaattaata
      121 aacatattaa ggctggaaaa aaaagatata gaataatttg taaatatcca ctttgtgtag
      181 cataatcacc ataatataat gcattataac catggactaa tgctatacgt acctctagta
      241 atttagaaac aaaaactatc ggtactacta ttatagaaat gatacaccct attttaaata
      301 aaaactttct atcggcatca gcatctccag attcatacca actaactttt cttagtgtgg
      361 tttttctcgt aaataagagt gctcctatgt gaaatacaaa catagaaata caagaatacc
      421 attttaccat tatcatatct gattcactag gaataatccc cgtaccataa gctactactg
      481 ttgtaccgat tccacgatct gttccaatac cgaatgccca cataatgcat tgcccataac
      541 taaataataa aaaaagagtg aaaaaaatcg tgtataatga aaatattgaa tctccttttc
      601 tccaatgcca tgtacagatt gcatataagt aaatcaatat tccaagccaa tttagccaat
      661 agttctgatt taatgaaaaa gcgcaaaata ttgtaaacgc gactaacgtt aacgaaaaca
      721 tcaatatatt tattttttct ttcacacaat atcctccttt tggttaaaag aactggttta
      781 aatattcgta ccatatattt gaaatatttt tagatgtcag ttcttttctt agtttaattc
      841 ctttttgcgc ttttcttacc acactttctc tatccaaaag taccctacgc attgcatctg
      901 ttaacttatc tagattatta cattcaacaa gatgacctat ttcatttcta tcacctaaat
      961 acattcttgc gccacctact ggacaatctg tagcgataac tggcactcca atagccagtg
     1021 cttctaacat agaattagat ataccttcat aatcagaaga aattacaaat cctgccgcat
     1081 tttgcatctt ttcatgtatt ttatttgtaa attcttttag atgaacaaat tctgttaatc
     1141 cattatggtt gatataactt tcaagctcgg tttttaatcc cccttctcca taaatatcca
     1201 gttcacactc atatccttca tgaataagtt ttcctattgc atcaatcatt aagggcaaat
     1261 ttttttgttt atccaatcta catgcagtaa taaatt
//
LOCUS       DACCXW010000254         1295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCXW010000254 DACCXW010000000
VERSION     DACCXW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.265"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            233..1165
                     /locus_tag="IVY71_002959"
     CDS             233..1165
                     /locus_tag="IVY71_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986935.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
BASE COUNT          468 a          177 c          239 g          411 t
ORIGIN      
        1 tggtatattc acgagtacag aactggctga tcttgaattt atttctttga gtaaagtata
       61 tacaatcaaa aaaatcgtat tggatttttt taaagaaaat gattatttga aaaataaaga
      121 aatcattatc ccagaatttg actcgcggaa catcttgcta gctttagtac gttatgtcaa
      181 ttgggaaggc tatgaaaata aaaatccgta tgtagaagaa gcttgtaacg agttgattga
      241 ttatgtagag actcattttt ttaaaagaag atattcggaa gatgaaaagt cctttatcat
      301 acgagaaata gagatcgcaa tcggaagaaa ggcatttccc cttggactga aagaagaaga
      361 taagaaaagc gcagaaacga agccgttatt tcaattagtc tgtcaaggat tggaagagca
      421 aaaaggaatt ctagacttac aagaagaaga tgtctattat attttttctt tgttcaacag
      481 tagaaattat accaatgaga acatggagct gttaagaaaa gatttcgaag ttgtttataa
      541 aaattttgtt ctcaatgacc tatcactgaa tgaattgatt ttacagatta ttagtatcgc
      601 tgaaatcgac gatgcagaaa attttatttt caagcaatcc tttttacaat ttgtccgtac
      661 gttgtgggca gatggacaag tatttttacc ggaaaggatc tatcttctat cagaaaaaga
      721 acaacttctg tacggaaaaa ttgtagaaat attggaagag tggaaaaaga aaaatcaaat
      781 ccaactaaga tggaatgaaa atttcattcg aaaattcgtc aaaagtcttt ctttgatcaa
      841 cagtgaacaa gcggggagac aggaaataga aatttttgtt gtttcagaaa gttcagtcaa
      901 acaatttttt tatcgaaccc aatttcattt gtatgcggaa gaaggcatcg ctgaaataaa
      961 tcctatttta tttcgttcat tagaagaatt gcctgatgaa tgtctatgtg cgaaaaaaag
     1021 agtggttttg tgcgatgctg ctagctatca aaatggatta gaaactgaaa agacaaagat
     1081 ctatccaatc tcattaaaag gtgcaaacat ccatttgtat catattgctc aggaacttca
     1141 cctattaaaa aaacatattt cttgatgaga ccttaattag gagaccttct acaaagcttt
     1201 ttaattgtct gtagaaggtt tttatttgag tcctgattcg tgcgaagatc tacttatata
     1261 acaagctttc tctgaaattt ttttaaaatg ctatt
//
LOCUS       DACCXW010000255         1289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCXW010000255 DACCXW010000000
VERSION     DACCXW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.266"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            143..1270
                     /locus_tag="IVY71_002960"
     CDS             143..1270
                     /locus_tag="IVY71_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7986936.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          452 a          222 c          251 g          364 t
ORIGIN      
        1 ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg cttctatcat tacaagctcg
       61 tttggtgtaa gatgggtata agtcatttat gttcactctc cttgtatgct ttagcgggta
      121 ttacaattgg agtgtaacat aaatggcttt tttatttgtc tcgcttaatt atacaaacgg
      181 cgaagatatc aatgatgatg gctcaagaaa aaaagtggag aaagttggcg gagacacccg
      241 accagaggcc gaagctgctt tacgaaaagt tttatcagat attgacgaaa caggacaata
      301 ctttttaggt acggatactc gagtaaaaca ataccttgat ttttggatgg aggaatacgt
      361 taaactaaat ctaaaataca atacctatga aaactaccga tttaccatca aaaatcatat
      421 aaacggttat ttaggaaaga aaaaacttac ggatctctcc cctgctcttt tacaaaattt
      481 catcaatgct gaatttaaaa agggttactc gaagaaaaca atgactatta ctcactctgt
      541 ccttaagaat gcgctgaata tggcggttta tccttgggga ttaatcaagc aaaatcctat
      601 gctgtatgta aaaataccaa aatatgaagc acgaccaacg actaaaaaag atctaaaaat
      661 catttctctt gaggactttg atcatatgct agaaattact cctgaaggac atcctttcta
      721 tattcctttg aatattggat tttatacggg aatgcgcgtt ggcgaagttt gtggtctgac
      781 gtgggatgat gtcgattttt caaatggaac aattactgta gagaaacaaa tggtaaagaa
      841 tgacggcgca tgggtatatg gtacgccaaa gacaagcagt tccaatcgaa cgatttttat
      901 tggacaaacc ttgctagcaa ttctgaaaaa acataagaaa caacaattag aaaatcgaat
      961 gaagtatgga aagctctaca ttgattcaaa tgcagtatgt acgaaggaag acggtgagct
     1021 agttacgcca agtgtagtga aatggaatac taggaggata tcgaatgcac tctccctctc
     1081 ttttaacttc cattctctca gacatactca tgctacactt cttctcgaaa atggcgcaaa
     1141 aatgaaagaa atctctgaac gattgggcca cagcagaatt tcaattacga tggatactta
     1201 ctcgcatgta acagataaga tgagaaatga aacggtcgat atcatggaaa atcttagaaa
     1261 gaattcttga tttttgccac cgaaaaaaa
//
LOCUS       DACCXW010000256         1285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCXW010000256 DACCXW010000000
VERSION     DACCXW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.267"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..616)
                     /locus_tag="IVY71_002961"
     CDS             complement(<1..616)
                     /locus_tag="IVY71_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7986937.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLR"
     gene            904..>1285
                     /locus_tag="IVY71_002962"
     CDS             904..>1285
                     /locus_tag="IVY71_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7986938.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYK"
BASE COUNT          429 a          200 c          246 g          410 t
ORIGIN      
        1 agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc gtaaatagct
       61 gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac ttcattgatt
      121 tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg taatgcttcg
      181 ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc ttagcgatta
      241 gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt tcctttgtta
      301 gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca caatcatagc
      361 atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact ttcaatatct
      421 gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag tgttcacaat
      481 gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct ttatattctt
      541 tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg agtaattttc
      601 tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc gactaaattt
      661 taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta gaaaatcgcc
      721 ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga tttcaaggtg
      781 tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt caccaacact
      841 agatattata gagccataat atgctgtata atatctagtg tataggctta agtggaggta
      901 tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat aagtgaatat
      961 ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc cttgtcaaaa
     1021 tttaatagcg atataaattt ggttatctta gacattcaga taccttttat aaatggttta
     1081 gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt gactgcattt
     1141 agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata tgtagaaaag
     1201 ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa gaattatgga
     1261 catttagaga agtttgaata taagg
//
LOCUS       DACCXW010000257         1272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCXW010000257 DACCXW010000000
VERSION     DACCXW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.268"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            854..1066
                     /locus_tag="IVY71_002963"
     CDS             854..1066
                     /locus_tag="IVY71_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986939.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          476 a          189 c          209 g          398 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aa
//
LOCUS       DACCXW010000258         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCXW010000258 DACCXW010000000
VERSION     DACCXW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.269"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            56..>1265
                     /locus_tag="IVY71_002964"
     CDS             56..>1265
                     /locus_tag="IVY71_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7986940.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVL"
BASE COUNT          392 a          252 c          246 g          375 t
ORIGIN      
        1 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
       61 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
      121 aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct
      181 tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg
      241 ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct
      301 ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac
      361 ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg
      421 cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc
      481 ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc
      541 aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt
      601 tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc
      661 ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat
      721 tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca
      781 gttgatcatc gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg
      841 agtgaaagaa accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa
      901 aaaattgaag cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa
      961 acgggtttgg cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact
     1021 gttagctgct tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc
     1081 ggtaaagaaa agagatcagt cactatttga ggattgttta actagagaca ttagcggtct
     1141 gccagaaacc tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa
     1201 tgcgcttcac tacagctatt caaacggacc attggaatgc ttaaacaacc acatcaaagt
     1261 attaa
//
LOCUS       DACCXW010000259         1252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCXW010000259 DACCXW010000000
VERSION     DACCXW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.270"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            33..905
                     /locus_tag="IVY71_002965"
     CDS             33..905
                     /locus_tag="IVY71_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7986941.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          376 a          230 c          302 g          344 t
ORIGIN      
        1 gatttcattc taaaataaag ggagaaatgt ctatgctata cggtgcaatt gaagcaggcg
       61 gcacaaaatt tgtgtgtgcc attggcgatg aagaaatgac gatcaaggaa agagtgagct
      121 ttccgacaac gacacctgag gaaacaatgc ctttagtcat tgacttcttc aaacaatacc
      181 aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc catcgcgatt
      241 ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat tttgatttca
      301 tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat gtcaatgcgg
      361 ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc gtttactata
      421 cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc gaagggttta
      481 gtcatccaga aatggggcat atgctcgtcg tccctcatcc aaaagatgac tttgctggca
      541 gctgtccatt tcatggcaac tgtttagaag ggatggccgc tggacccgcc atcgaaaacc
      601 gcttagggaa aaaaggacaa gaggttgcag aagatgatcc ttattgggaa atcgaagcct
      661 cctatattgc tcaatgtgcc tacaatacaa ccttgatgtt ttctccagat gtgatcattt
      721 ttggcggtgg tgtgatgaaa caacgccata tgctgcaaaa cgtccacgac gcatttgcaa
      781 aattggtcaa tgggtatgtc gaaacacctg agttaagcaa atatatcgtg accccagcat
      841 tagaagacaa cgctggaacg ctcggttgtt tggcattggc tcgagaagcg gtcttacatt
      901 cataaacgaa aaaaggctct tcgacattta gtgttggtag agaaagatac aactgaaaat
      961 ttagtcataa ccagttaggc agagtgctta actggttttt gttttacttg gaagatcaag
     1021 ccttgaatga gataaattct tgctcgaaag ttatggaaat ttcgatagcc gtaggccact
     1081 tgtattgtgc aatagttttt tacaaaaaaa agggggtgcg gatatttttt tggacctttt
     1141 taacaaatag gttctgttgc aaagttttaa atctactatc agataaggta gaataataga
     1201 aaaagatagc aggaggaatg acgatgagtc attttaaagg aaagcaattt ca
//
LOCUS       DACCXW010000260         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCXW010000260 DACCXW010000000
VERSION     DACCXW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.271"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            200..742
                     /locus_tag="IVY71_002966"
     CDS             200..742
                     /locus_tag="IVY71_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986942.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IVY71_002967"
     CDS             832..>1246
                     /locus_tag="IVY71_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP7986943.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACCXW010000261         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCXW010000261 DACCXW010000000
VERSION     DACCXW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.272"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            386..634
                     /locus_tag="IVY71_002968"
     CDS             386..634
                     /locus_tag="IVY71_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986944.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            1137..>1246
                     /locus_tag="IVY71_002969"
     CDS             1137..>1246
                     /locus_tag="IVY71_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7986945.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          440 a          209 c          189 g          408 t
ORIGIN      
        1 tatttttata tgttctcatt atattacctc cataaattcc taattgtctc ctttaattca
       61 tctataattt catccacgat attttaatcc gttctacaat gcatatctga tatgcattga
      121 ctgtctaact catatgtgaa tgatttataa aatatcccaa cgtctcatct aaagaaatgt
      181 tgatcgtcag caaggagcta aaaaatacga atatatttct tttgtagtaa aatcggactg
      241 gtttgcatat cattttcttc gtaaatatca aaatcatgtt aactactatc aattactaat
      301 aaaaactacc actttaagcg ttaagaactg ttaagtttag ttaagtgagg atagttcaca
      361 aaatccattg ttaaacttca cgaaagtggt aaaactcaaa atgctttata taggattaca
      421 gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa aaattgacga
      481 tgacacgttc cttacggctc atcagatgaa acagctacaa aaacgaaatg atcaactcga
      541 agaaaattta attctaaaaa agcgattgcc atattcacgc ctcgctcgac gaactattaa
      601 aggtcaagat ttacctgaag atcatccttt ttaggaaatt gaagcttttt atgttgcaca
      661 atgcatttat aatactacaa tgatgttttc accagatatt cttctagggt gcggtgtaat
      721 gaaacagata cacttgcaaa agaaaattca ccgcgaattt actaaaatta tgaataatta
      781 tgttaatctt ccgaaatttt tttccaaaat taagggatca agctggtgtt atagattgtt
      841 taaggttggc aaaaaaataa cttcttttta aacaattata gaaaaccgat actctgttaa
      901 gttggttgac acggtgtcga tttttatgac tttcttattc tgaaacttca ctaacggaca
      961 gtacctaaaa agtctatagt atggacgttt acgagtacta ttttatcaaa aatgctgctc
     1021 atttcaactt atatgtttta gtgaactacc cccacttagc ttggataggc tacaattaac
     1081 tagacagaaa aattaaggtg tgtagactag aagaaaacat accaggagga atttttatgt
     1141 ctaagagaac acgaagaact ttttcacaag aattcaagca acaaatcgtc aatctttact
     1201 tagctggaaa gccacgtgta gaaatcattc gagaatatga actaac
//
LOCUS       DACCXW010000262         1240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCXW010000262 DACCXW010000000
VERSION     DACCXW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.273"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..383
                     /locus_tag="IVY71_002970"
     CDS             <1..383
                     /locus_tag="IVY71_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7986946.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="IVY71_002971"
     CDS             complement(367..960)
                     /locus_tag="IVY71_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP7986947.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
BASE COUNT          379 a          246 c          215 g          400 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttctatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcacatttt cgggtattaa ttcgagaatc
     1201 acacctataa atttattact atataaggga tacttaaacc
//
LOCUS       DACCXW010000263         1226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCXW010000263 DACCXW010000000
VERSION     DACCXW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.274"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            82..>1226
                     /locus_tag="IVY71_002972"
     CDS             82..>1226
                     /locus_tag="IVY71_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7986948.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGIT"
BASE COUNT          407 a          239 c          237 g          343 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcac
//
LOCUS       DACCXW010000264         1225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCXW010000264 DACCXW010000000
VERSION     DACCXW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.275"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            316..570
                     /locus_tag="IVY71_002973"
     CDS             316..570
                     /locus_tag="IVY71_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7986949.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IVY71_002974"
     CDS             571..888
                     /locus_tag="IVY71_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7986950.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          426 a          202 c          246 g          351 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaat
//
LOCUS       DACCXW010000265         1214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCXW010000265 DACCXW010000000
VERSION     DACCXW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.276"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            70..1026
                     /locus_tag="IVY71_002975"
     CDS             70..1026
                     /locus_tag="IVY71_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986951.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="IVY71_002976"
     CDS             complement(1080..>1214)
                     /locus_tag="IVY71_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7986952.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DACCXW010000266         1207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCXW010000266 DACCXW010000000
VERSION     DACCXW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.277"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            155..1195
                     /locus_tag="IVY71_002977"
     CDS             155..1195
                     /locus_tag="IVY71_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7986953.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          487 a          175 c          200 g          345 t
ORIGIN      
        1 aaggtagtac ataattcatt ttatttctga ttttaggtag tatttaatta aaaaacagtt
       61 ttaatgcgtt tgcatttttt atttatcttt gctattttcg aaaagagtag gtaattttac
      121 ataaataaaa caacgtgaaa gtagaggatc tacaatgact gattttaaat tttttaaggc
      181 ggatagagtt tacaacgaat tattttatca atttccaaaa gtctttattg tttctgacga
      241 atacaaaaaa atgaaagatt caactaagat tgcctatatg cttttaaaag caagattaga
      301 gatcgcaatc agcaaacggc aaatcgatga agaaggtaat gtttatttta cttatacgac
      361 aaatgaacta tgtagagtat taaactgcca aaaacaaaaa gcgatagcaa tcaaaaaaga
      421 gttggaatcc tttggtttat tattacaaaa gcagatggga tttaacaaac agttagggaa
      481 aaataatcct aatagactat atctagcaga attaaaagtc tcagaaaatg atatctactt
      541 actcgaaaaa tttgatagag agaataggga aaacgttgat aaatcagagg gtatgaaaat
      601 catacccacc ctcgacgaaa aatcagacgc tgaatccctt ggggctcaag agggtatgaa
      661 aatcataccg tgccaaaacg ttgataaatc agagggtatg aaaatcatac cagaacttaa
      721 taataatata ttagacacta atagacacaa tatagacact gaaaaagacc gcctacaaga
      781 tcaattgttg ttagacaatt ttgagacaat tatgacaaac gacagcattg ctacgtttgt
      841 ccctgaacga gtattaaatt tgataaaaac attttcttca agttacagtg aagctcaaaa
      901 aaccgtccag actattcata atgcaaagaa aaaagctgaa atagaaagtg atatttcgat
      961 agtttttgaa gaactcgata gttattatgt caatgcagaa caagaattat acacgacact
     1021 gttaaaagcc tatcaaaaat taaaaaccga aaaagtcgaa aatatccaga acctgatttt
     1081 tgtctatgta aaaaattggt ttatcgaaaa accaatagct gctaaagtat caagtgaaaa
     1141 acgtttgaat tatgcaagct ccccaagcac tattacgaaa gactggttag agtgaaactt
     1201 tactgac
//
LOCUS       DACCXW010000267         1207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCXW010000267 DACCXW010000000
VERSION     DACCXW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.278"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            64..324
                     /locus_tag="IVY71_002978"
     CDS             64..324
                     /locus_tag="IVY71_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986954.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(686..925)
                     /locus_tag="IVY71_002979"
     CDS             complement(686..925)
                     /locus_tag="IVY71_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986955.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          429 a          166 c          221 g          391 t
ORIGIN      
        1 ataatcatat tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga
       61 aatatgaata acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg
      121 aaacaaaaag tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta
      181 gacgaatatc atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca
      241 aaatatactc acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa
      301 tatgaacata ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc
      361 aagcttttga aggcacttta tttagttggc agtgattgtt catgattttt gatttattca
      421 aaaggttcgc ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa
      481 cctatactgc cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca
      541 tccgatccaa tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg
      601 ttatcgtaaa gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca
      661 ttatttgctg aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg
      721 ctgacagttt ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta
      781 ccggagaatt tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat
      841 ctgaaggtat gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata
      901 aactgataaa cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac
      961 acctatgctc agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat
     1021 tagtcttagt aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt
     1081 taccatctgt attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg
     1141 aaaacgtagg tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg
     1201 aggatag
//
LOCUS       DACCXW010000268         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCXW010000268 DACCXW010000000
VERSION     DACCXW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.279"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1076
                     /locus_tag="IVY71_002980"
     CDS             <1..1076
                     /locus_tag="IVY71_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7986956.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACCXW010000269         1184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCXW010000269 DACCXW010000000
VERSION     DACCXW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.280"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            65..340
                     /locus_tag="IVY71_002981"
     CDS             65..340
                     /locus_tag="IVY71_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986957.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            767..>1184
                     /locus_tag="IVY71_002982"
     CDS             767..>1184
                     /locus_tag="IVY71_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7986958.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENS"
BASE COUNT          446 a          205 c          177 g          356 t
ORIGIN      
        1 ttgttttcat ataaccaaag gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa
       61 aaaaatgaaa cttgaggata aaaaagtcat tttgaagaaa atcaaacgac taccagacca
      121 aatagatggc tatacgatct gttctttcaa aaaagacagg agtataaaag cttatacgca
      181 aaacacagaa aaaggaataa tctatttcct acatgaagaa ggttacgata cccaaacctt
      241 tatgtttact gaaaaaaaag aatttcataa acagatgaaa aaactgattg aaaaagaatt
      301 tccaagaagt cataaactct atgtgaatca gcaattttaa cttctaactg ttctttatta
      361 attaaaaatg ttctgtaata aaataaaact ttataacaac tataacaaaa ccgaatgcaa
      421 aacagaagtt ctataagtta taatcatagt tgttaggtga ttcatctagt aaaaacacca
      481 gatttcaaat ttcacacaat ttaaactaga tcaatcatca accttcttca aattgttaga
      541 accctttccc gaggagctgc agcataagca aaaaaacaaa caggaagcca aatggctaag
      601 acattttttc tattagtcta gttgcttctc gtcgcaagtt taagacttta gaaagttatt
      661 tctaatgatg tttgggttgt tttgatcaat gcttatgcac agctccttac ctattattta
      721 tctcaacaga tctaacaaat taatggacta tttaaggagt ttttttatgc ttacgatcta
      781 ttcttctcgc acacatagtt accatctgat ggcaaactgg ctgaaagacc atcaaatgcc
      841 ttacagagaa cgaattttca cacctaaaca gcctttaaca aggaaagaga tattttattt
      901 attgtcatta acagaaaacg gatttgacga tctattagct aaacgctcga ttgattataa
      961 aaatttgaaa gaccaaatcg accaatgtac gacctcagag cttgtcgatc tgatcgtctc
     1021 gcattttact ttgttacaac gtcccatcat tactgacgga aagaaattat tagtcggatt
     1081 caatgaagat agcatgcgtc tttttatccc tgtacaatgg cgcagaagtc aactgaaatc
     1141 tgtctataac gattttacat gggtaagcaa tctagaaaat tcta
//
LOCUS       DACCXW010000270         1183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCXW010000270 DACCXW010000000
VERSION     DACCXW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.281"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(23..1114)
                     /locus_tag="IVY71_002983"
     CDS             complement(23..1114)
                     /locus_tag="IVY71_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7986959.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT          378 a          224 c          181 g          400 t
ORIGIN      
        1 ttttataagt aaaaaaatac aattatactt cataaaaaga cttataccac tgcgcaaatt
       61 tacgaagccc gtcacgcaaa cttgtacttg gtttaaattc aaaatctcgt tctaacgcac
      121 tagtatctgc ataagtaact ggcacatctc ctggttgcat tggaactagt tcggtatgag
      181 attcaaagtc gtaatcttct ggtagcaccc ctgctgcaat tagctcttgt tgtagaatat
      241 ctacaaaatc tagtaggttt tccggcgtat tgttaccaat attataaata tgatatggtg
      301 ggattggtag cccatcttcc ccatcaattt tctctggagc gcactgcata accctcttta
      361 ctccttcaac aatatcatct atataggtaa agtcacgctt gcaattccca taattgaata
      421 tttggatatt atcaccagca cgaagtttat cagtaaaacc aaaataagcc atatctggac
      481 gcccagcagg accatatact gtaaagaaac gtaaccctgt agacggaata ttgtaaagtt
      541 tagaatatgc atgtgccatt gattcattac ttttcttagt tgctgcatac aacgatactt
      601 gattgtctac tttatcctct gtagaataag gaattttctt attagtacca taaacggaag
      661 acgatgaggc atacactaaa tgttccacac cagattctcc attatcatat gaatgacgac
      721 aagcttcaag aatattatag aatcctatta tgttagattc gatatacata tctggattag
      781 taattgaata acggactcct gcctgagctg ctaaattcac cacgatactt ggtttataat
      841 tatcaaagat gaaatcaatt gttgctttat cagcaataga acctttgata aaattccaag
      901 tagatcctgg atggttcttt gcctccgttt ctatctctga taatctatag ttcttaatag
      961 agacatcata gtaatcattc atattgtcaa tgccaatgat ttttattgat gactgagtac
     1021 gtaataactc aagaaccaaa tttgatccaa taaaaccggc tgcgccagtt attaatattg
     1081 ttctatttga taaggacgtg tttaattcca gcatttttta gcactcactt tctttttttc
     1141 ttatgaatac atatttgtta tatattaaaa aatcttattt aga
//
LOCUS       DACCXW010000271         1169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.282, whole genome shotgun sequence.
ACCESSION   DACCXW010000271 DACCXW010000000
VERSION     DACCXW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.282"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..393
                     /locus_tag="IVY71_002984"
     CDS             <1..393
                     /locus_tag="IVY71_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986960.1"
                     /translation="LLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKEF
                     GDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAI
                     SIKDLKELLNSKRNELKQIKETNSVELE"
     gene            407..1096
                     /locus_tag="IVY71_002985"
     CDS             407..1096
                     /locus_tag="IVY71_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986961.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
BASE COUNT          413 a          158 c          241 g          357 t
ORIGIN      
        1 cttttgaatt tagattctgt taattcaaac gctcaaataa aagatcaaag agtagctgaa
       61 cataataata aaaaaatgag tttgaccaaa aatatctatt ctatgtcaga tgaagaattg
      121 tataaagaat ttggtgattt atccggggaa cagttagaag aaagtattcc aggaattagt
      181 gactcagata aaattgttgt cttgtctgct ggtgggcaat taatgggaga tggcaaatcc
      241 gaattagtta gttataaaca aaatcacaga cagtttattg atgttaatac tgattccaac
      301 gctatttcga ttaaggatct aaaagagctt ttaaatagta aaagaaatga attaaagcag
      361 ataaaagaaa ctaattcagt ggaattagag taaaggaaga ttcattatga aaaagatttt
      421 tggtgtttct gttgggctac ttatgatgtt aacatttgga aatgcagtat atgctgctga
      481 gaaagaagtt atttcagatc cagagcagta cgcctcacga tactttgctg attttgaaga
      541 agaagataaa gtacttgcat tgccagaagg tggtttttta aaaggggaag ctaaaattgt
      601 atctatggat aatccagata taattcaagg ttcatataat tcagaaactg atcctgcttc
      661 agtttcagtg acagaagcaa aaaaaattgt tgctgaaaaa ttcaaaaatg atattgaaac
      721 tactccaatt atgcctcgag gagcatttcc acccgatgtt ggtactagcg gacaccttct
      781 tgtactagca ggaggaggaa gctataaatc acagccattt agtggtgcgg gatggcgttt
      841 tggaggtatg ggtgtaaaac cagctgatgg tacaggcaac tttttacgtt ggaaatccta
      901 cattgacgat ggaagagtag gaaatgtaaa tcaagccaac gcaactaaat ctggttctat
      961 tcaaggaatg gcattgtatg ctcgtgaagg attcaaatac tttgcaccaa acgggcctgg
     1021 ttggacaatg aatataggta tttattacac ttataatcct gttgcaggta cgtattatgt
     1081 tgttcaaaat atttaataaa acagcacaaa gagatttctt tgtgctgttt tattattttt
     1141 agttgttgac aaacactaaa atataaaat
//
LOCUS       DACCXW010000272         1166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.283, whole genome shotgun sequence.
ACCESSION   DACCXW010000272 DACCXW010000000
VERSION     DACCXW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.283"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..176)
                     /locus_tag="IVY71_002986"
     CDS             complement(<1..176)
                     /locus_tag="IVY71_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7986962.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKV"
     gene            305..430
                     /locus_tag="IVY71_002987"
                     /pseudo
     CDS             305..430
                     /locus_tag="IVY71_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            558..1109
                     /gene="raiA"
                     /locus_tag="IVY71_002988"
     CDS             558..1109
                     /gene="raiA"
                     /locus_tag="IVY71_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7986963.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT          403 a          213 c          227 g          323 t
ORIGIN      
        1 actttcccgt ttccatctac cacagtagaa ccacaatttc tacaggtatg gggagcaggt
       61 gagaaaacag catcgacgat caacgtcttt ttcttctgaa aggtctcgta agagacctct
      121 gtaatcatca aatctttctc tattaatcgc agcatttttt tgatagaatc attcatatag
      181 catatcgtcc tctcagttgt ttattttgtc gtgatttaat catactagag aacgatatgt
      241 tttttaatac ctaaaataaa aatggggctg aagaatcaat tctgattcat cagtcccata
      301 aattatagag cctaaaaaaa tattgattat tgacgatgtc tatacaacag gacagacgct
      361 ttttcatgca gcagattgct tgcagaacgc acagccgaaa atcatccgaa ccatttcttt
      421 ggcaagatga atcagatgtg gaaatttgat agcgatttaa tttgcaaata acctagagtt
      481 cgatataata gaattaagaa gagggaaaga gtggtccttc tccttcttga ttgtggtaga
      541 cgaaaggggt aatttttatg tttagatata atgtacgcgg cgaaaatatc gaagtaactg
      601 aagcgattcg cgactacgtt gagaaaaaag taggcaaact agaacgctat ttcagtgacg
      661 cacccgatgc aacggcgcat gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg
      721 aagtaacgat cccattacct tatcttgtat tacgtgccga agaaacttct ccagatttat
      781 acgcaagtat cgatttggtt gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa
      841 aaattaatcg taaatcccgt gaaacgggca tgaatactgc agatgctgct gctttattca
      901 atgaagagga cgaaaacgaa aatgattctg aattagatat cgtacgtacg aaacgccttt
      961 cattgaaacc aatggatagt gaagaagcag tcttacaaat gaacatgctg ggacataact
     1021 tctttatttt tgaagatgca gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg
     1081 gaaaatacgg actgattgaa acagactgat cagccttact taaataacaa cgaaaacttg
     1141 cagcagctac tgacagctgc cgcaag
//
LOCUS       DACCXW010000273         1161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.284, whole genome shotgun sequence.
ACCESSION   DACCXW010000273 DACCXW010000000
VERSION     DACCXW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.284"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            41..472
                     /locus_tag="IVY71_002989"
     CDS             41..472
                     /locus_tag="IVY71_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986964.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            474..611
                     /locus_tag="IVY71_002990"
     CDS             474..611
                     /locus_tag="IVY71_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP7986965.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            648..941
                     /locus_tag="IVY71_002991"
     CDS             648..941
                     /locus_tag="IVY71_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986966.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            938..>1161
                     /locus_tag="IVY71_002992"
     CDS             938..>1161
                     /locus_tag="IVY71_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7986967.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          436 a          167 c          240 g          318 t
ORIGIN      
        1 tcatttgtta tcacttattc aatttaaggg aggaaatcta atgaaagtag tttacaaatc
       61 aatcaagcct tacggattcg agcaaatcat tttgaacaat caagaaaata tccctgaaaa
      121 ctgtacagag attaaaccac cagttcctaa ctggagacca agatttgatt ttgataaaaa
      181 acagtgggtt gagttagcta ctgaagaaga gaaaaaaggg aatgctgttg atagcgtaga
      241 tgaattggct aatattaagg cattatacga aacgctgaag gcagaaaatg atgaattgaa
      301 acagctaaat tctaaagcaa tgctaaacaa tgtagcaatc aagcaagaaa atgtcttatt
      361 gaaagaaaag tcagaaagtt tagcgcagtt gaattcaaaa acgatgcttg cttctgtaca
      421 aaataccaag gaaatcgaag aaatcaaaaa acaattacaa ggtgggaagt aacatgtatt
      481 catatgaaga tatcaaacta atgtatgact ggggctgttt cactaacgaa caagtaatgg
      541 ttttcgttcc attatgcatt acagacgaag aagcagataa aatcattaat aaagataaga
      601 gcgcatctta attgatgcgt ttttatttaa ggtaaaggag ttgtcacatg attaatttag
      661 gggaatgggg aatgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
      721 ttagaccgat tgctgcatct ttctcgaaaa ttactgagac tctttcaaaa gtaagccaca
      781 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
      841 aagaactaaa gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca
      901 cgcaacaaat caaaactttg tttagggaga gaagaaaatg aataataaaa cgttcgaagt
      961 actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg
     1021 taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc
     1081 gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag
     1141 cgaagaagga ggaaacaaat g
//
LOCUS       DACCXW010000274         1156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCXW010000274 DACCXW010000000
VERSION     DACCXW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.285"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            237..>1156
                     /locus_tag="IVY71_002993"
     CDS             237..>1156
                     /locus_tag="IVY71_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7986968.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKR"
BASE COUNT          397 a          183 c          249 g          327 t
ORIGIN      
        1 aggagtgcta taatcaaaga aaacgagaaa aaagagcaaa ataaaagccg aacggtgttg
       61 gaagcaccct tcggctaatt acggaaacta ctcacctagt tccgtaactc aaattcatta
      121 tacgaatatt tttaggtttc ttcaagctag agatttttgg atatggattt ttagagttac
      181 gtgaactctg gaaattcttt ccaaaaatct cttttttctt tgaggaggaa caaaatttga
      241 gtaaacgtgg cacgatcata aatgagtggg aaaaacttaa ccgcatatct aacaggcggt
      301 taaatgcaca aactatgtgc acccccttaa aaatgcaatg gtcaattgac cgaattacca
      361 ttgttggtac attaagggaa aatatttatt atcacactcc ccatgatgtg ttgattttag
      421 attttgaaca gcttatgcgt ctaaacgaag gtaacggata tttaaaagcg gttggaaata
      481 atggctggca attactagac caacatgagg aaaatatagc ttatattgag attcttaagt
      541 ttcaagaggg aaaagggcgg attgacttta accccaataa aattagccaa tttctggcgg
      601 gctcgatgaa gaactttatc catgatttat ttttagaacc acatttctca cgtgcagata
      661 ttgcttgtga cattattgac gtgccagatg atttcattac ccaatatcgg gtggttgatc
      721 ctgtttcttt caaaccaatt tacggtcggt ctgggaaact ggaaacagca tattggggaa
      781 gtcgtgctag tgaacgacag attcggatgt ataacaaaaa attggaacaa gaaagaaaac
      841 ggaaagttgt tccgaaagaa atcgatactt ggtggcgact agaattgcaa ttgcgaaggg
      901 ggaaagcaac tgattggcat gcaatggttc atgaaagttt agatagcttt gcaagtcctc
      961 actatttgcc agaagatgtg aaagtaactg acagaatgat gattactgct cttataacgg
     1021 aacatagtta ctgggggcaa attcatagaa attcaaaata taaatatcgt gaattgttaa
     1081 agcaagaaag tcaaaatgat gagttgacga atcatttgcg agaaacattt tcggagtcgg
     1141 cagatgattt gaaacg
//
LOCUS       DACCXW010000275         1118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.286, whole genome shotgun sequence.
ACCESSION   DACCXW010000275 DACCXW010000000
VERSION     DACCXW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.286"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            8..481
                     /locus_tag="IVY71_002994"
     CDS             8..481
                     /locus_tag="IVY71_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7986969.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            490..717
                     /locus_tag="IVY71_002995"
     CDS             490..717
                     /locus_tag="IVY71_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7986970.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          351 a          185 c          191 g          391 t
ORIGIN      
        1 gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta taagtgccgt
       61 atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa aaggattcaa
      121 ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc tcctagggat
      181 cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc aagctttttc
      241 cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta ttggtttcgc
      301 cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg ctccaggttt
      361 gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat taacaagcgt
      421 gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt tgacagtgtg
      481 aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa aaaaatgagt
      541 gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat tctaaaaaat
      601 ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt tttggagtgt
      661 caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga agaataaaat
      721 aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga gacttttaga
      781 agttatttac gtctattatt cagatttttt atttcgattg aataccgttt tatctataaa
      841 taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact atcacttttc
      901 ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat aatactttat
      961 tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt attacaggcc
     1021 attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg tgttaactct
     1081 tactccattc ttttttagta agctcccata tatactgt
//
LOCUS       DACCXW010000276         1095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.287, whole genome shotgun sequence.
ACCESSION   DACCXW010000276 DACCXW010000000
VERSION     DACCXW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.287"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(296..712)
                     /locus_tag="IVY71_002996"
     CDS             complement(296..712)
                     /locus_tag="IVY71_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986971.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(715..999)
                     /locus_tag="IVY71_002997"
     CDS             complement(715..999)
                     /locus_tag="IVY71_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986972.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          339 a          173 c          180 g          403 t
ORIGIN      
        1 aatcctgcat tacgagaact aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa
       61 gcaaaaaagg gcaataaaat cgccaaatta gaatttactt tttcaagcct aacgtcagat
      121 ttgccaaaga tttctctgcg taactggtta gaagaataaa taaaaaagaa ctcgcccccc
      181 ttacattaag caagtagaca agtcccttac aaccgactaa gattgctagt acattctagc
      241 atattgtatg gggcttctaa ataaaaaaca gataggaata actcctatct gttttttatc
      301 gttttagaaa atttgttggg tcgaaatttc tagaagtgac tgtttgactg gtttcttttt
      361 gatgattctt tggtcgattt cgatttgatg gatttagttt agcttcgatt ttttctttca
      421 cttcttgata tactttagaa tttgtgccaa taagaacgcc tgtatctttt agatataaat
      481 cagcaaattc tgcgatgatt tcagtcatga tggaaatttc ttttcccata gcatttagtt
      541 ttgcctgatt gctggtagtg gtcgtgttgg tatcgggttc ataactcata cataaaaagc
      601 gaatggcttc cgagtagtta ttgatatctg gatttttttc gatgatggaa tctaaaacgt
      661 caacatgact agggtacaaa gtaacatgtt tttggacaga agtttttctc atggttatat
      721 cattcctccg ataagcattt caatgatttg attatttgtg tagacatact cgcttaatat
      781 ttgagtattt tctgtttgga tagcaataaa tttatcgagc aataaatcga attggtcttt
      841 tttgtaagtc gtaagttgac ggtcaaaatc actttcgatt aaaactttta gatatttatt
      901 tcgactccat ttttctcctg ttttattcgt gagttcttgg caacgtttgt ctattgttgc
      961 aacaaacttt ggatcaatat tccgaactaa aatgtccata aaaaatctcc ttttgcaatt
     1021 atgtataatt tatgtatacc atacaaatac tatatttgta tagctgtttt ttgccaaaat
     1081 gctatacata agcta
//
LOCUS       DACCXW010000277         1082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.288, whole genome shotgun sequence.
ACCESSION   DACCXW010000277 DACCXW010000000
VERSION     DACCXW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.288"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            134..1069
                     /locus_tag="IVY71_002998"
     CDS             134..1069
                     /locus_tag="IVY71_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986973.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          405 a          129 c          192 g          356 t
ORIGIN      
        1 acatgaaaat atacatccct gctagactat ttacaacatt ttactttgta cgtattcttg
       61 ttttaaaagg aatgggataa aatataggta caataactat ttgatgctaa atataattaa
      121 tggaggagag atagtgaaca tacttaatga aaaaagatat gtatggtttg ctggtaaaaa
      181 atatattact actttcaaaa gattatccga gttaatgcag cagactgtta aaagtgctat
      241 gcctgaagga tacaagttta attttgttca atcttcagta tctactgaaa gtatctattt
      301 ttcaattctt tctgagagta ggctttttca ttttagaata agttctcatg taaagaaaac
      361 tgatgttgat atatatacgt tccgtacaaa taattataaa aatttaaatg atttgagtag
      421 aagtatcagt gaatatctca aggggaatag ctatctcaag attcgatttt cacatatatt
      481 aattttacta ccatatttga atgagaattt caccctaatt caagtagata agtctttcat
      541 tgcctcaggt aatcaaggaa atatgaatat tacaagtaac aaaacgtcac gtgtgctatt
      601 ggcgttgatg ggttacggaa ttctttatag agatgaccaa aatggccaga ttctaataaa
      661 tcgggctggg cgttcattat taatgaaata tagaaaattt ataccggaag attttcaaaa
      721 cttacagtta gaaaacatgc gtttagaaga tttatttaga ttagcagaag atgataagta
      781 tagatttgaa gttttgaaaa ttcaaaagaa tgaactattg aataagttat atcctgagaa
      841 tgcgataaaa gcacattttg gtgatgctaa tcatatgtat agttggttca ttcctaaaag
      901 atttagaggg aaaatcaatg ctggagatca agtgaaagta ctcaatcgaa atagtacaga
      961 tgtcgtaatg ataacagata tctatgtaga agatgaagat ataatcaaat atatgaagcc
     1021 tgtgataaaa cttgttaata aaaataaagg aacccgtggt gctagttaat tttttcctag
     1081 ag
//
LOCUS       DACCXW010000278         1056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.289, whole genome shotgun sequence.
ACCESSION   DACCXW010000278 DACCXW010000000
VERSION     DACCXW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.289"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1056
                     /locus_tag="IVY71_002999"
     CDS             <1..>1056
                     /locus_tag="IVY71_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7986974.1"
                     /translation="AEYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMS
                     SVLSLATLSLIVFLLIGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTIN
                     FNHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMI
                     KGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIG
                     IILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKF
                     MAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNM
                     IFIPRLGIIAAGYTTVFT"
BASE COUNT          322 a          134 c          179 g          421 t
ORIGIN      
        1 agcagaatat ggtttgatta ataatttcac ttcatttgta tcaatttttt cgattcttgt
       61 tgggctaagc ctaaattcgg caattaataa tgcaagtttt gactataaag atgacataaa
      121 tgaatttatg tcatcggtac tttctctagc tacactttcg ttaattgtgt tcttattaat
      181 agggaacata tattttttgt tttcaaatgc tttttttgat atgaaccaac ttgtatttaa
      241 tttgatgtta ttacaaagtt ttgctagttt tgtaattagt tatatccagt cttattttac
      301 gattaacttt aaccatttta aatatttgat tttatctttt atatctactt ttttgaatat
      361 tgcaatctct gtagctttga tgcagacatt atattttgat gataaatatg ttggcagagt
      421 agtcggcgga acactgtcat ttgtgttgat aggtttagta atttatttaa gaataatgat
      481 aaagggtgct catttttttg ataaaaaaca gtggagatac gctttaaaaa tctctttgcc
      541 gctaatccca cattccttag caaatatagt tttagcacaa ttcgatagaa ttatgattaa
      601 ttcttattta ggtcctgctg cggggggaat atattcctac attagtaaca taggtattat
      661 tctaagcgta atatggatgt ctactaataa cgcctgggtt ccatggttct atggtgagat
      721 gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac tattatttag tagtctttac
      781 tttgattacg atgattgtaa tggtagtttc gattgattta gcaaaattta tggctccgaa
      841 agactattat tctgggttat ctttagtggt tccgataacc ttaggctatt tctttcagtt
      901 tttatatagt ttaccagtta atgctgaatt ttatgagaaa aaaacatcgt ttattgcgat
      961 tggtacagta gcaagtgcta tagttaatat actattaaac atgattttca tacctaggtt
     1021 aggaataatt gctgctggat acacaaccgt atttac
//
LOCUS       DACCXW010000279         1042 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.290, whole genome shotgun sequence.
ACCESSION   DACCXW010000279 DACCXW010000000
VERSION     DACCXW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.290"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            101..841
                     /locus_tag="IVY71_003000"
     CDS             101..841
                     /locus_tag="IVY71_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986975.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          382 a          185 c          200 g          275 t
ORIGIN      
        1 ttcatcgcta ttttcttaaa ataatcagcg cattgctact gaaaacaatg aatctgtgct
       61 attattgaga aaagttccat ttttgagaag gatggtttga atgaaagaaa tcagacgaaa
      121 acatttccgt tttcctgcgt acgatgatga gttaggcgta aaattaacca acgacaagaa
      181 gcgagtatta tttgacggac aagaagatct tttgactgaa cagaatcagt ccccgtttga
      241 acagatgaaa gaatttcatt tcacgaacac gaacaccgag aaaatccgtc gcaagcgtcg
      301 tgcaacaaat ggattgaagt cagctccgcg taaggaaaat ttggaaaagc ataaaggaaa
      361 attaccggat tataccggcc attaccaatc agaaatcaca tcgacaggac gaaaaaaact
      421 ttttggtgat tcctctcgtt ctgtcagaca aagtgaaaca aggacagagt ccaagcatac
      481 tacacgaaac gcatctgttg acacgaaggt gcgggagcgt tcttattttg ttcctaaata
      541 tgtacccgca tcgattatcc cagaccctag agaaagcaaa atttctgagg atcatttaat
      601 cgaatcgatg aaaaaaacgg atgactctta tctgttgttc gatacagaac cgacgccgta
      661 tcaagaaaaa agaaatggtg agccgtctgt acgaacattc aatcgaacaa aaacgttaga
      721 ggaaacaaag cagtcagaag aaaaaaaatc gaaaaaaagc catggtatct tagaacgctc
      781 attacaagga ttgatcgatg atcaaggaaa ctctttggga gaaaatagct attttaaata
      841 agtgatatac aaatttatat tcggctacag ttggaaagga aacgctgcgg cgtttcctta
      901 ttttatactc tatccgaaag tttatatcgt atcctcaaat ttttttgagt ttaagcgaat
      961 tttttctccc aataagtaag aaaataatgg caaaataaaa aaacaaacta ttttcgcaga
     1021 tcgtttgcaa aaatagttta tt
//
LOCUS       DACCXW010000280         1039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.291, whole genome shotgun sequence.
ACCESSION   DACCXW010000280 DACCXW010000000
VERSION     DACCXW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.291"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            24..419
                     /locus_tag="IVY71_003001"
     CDS             24..419
                     /locus_tag="IVY71_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7986976.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            416..>1039
                     /locus_tag="IVY71_003002"
     CDS             416..>1039
                     /locus_tag="IVY71_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7986977.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMM"
BASE COUNT          400 a          118 c          211 g          310 t
ORIGIN      
        1 aatcattatt tagttaaaaa gatatgtttt gcaaagtatg tgatagaaaa gagggattct
       61 gtggcttcag aatagatatc aaagaattgg caaaagtttc tgatattttt ttatttacta
      121 cattacaaga aggaatgcca agatctatga tggaagctat ggcaagtgga ttgccatgta
      181 tagcaagtaa aatccgaggt aatgtagacc ttttagtgga aggaaaaggt ggatatcttg
      241 ttccagctaa agatgcagaa ttagtagcag agtgtttgag agaactaatc aataataaat
      301 ctcttagaaa gtatatggga aatgagaacc tttccagaat taaagaattc gaggttagta
      361 tcgtaaaaaa aagaatcaaa gaaatatatg aagaagttat aggtgagagt gtaaaatgaa
      421 aaatccaatg ttttcaataa tagtacctat ctattgcgtt gaaaaatatt tgaaagattg
      481 cttagattca ataagaaatc agacatttaa aaattttgaa gcaatactag ttgatgacgg
      541 tagtcctgac gaatgcccag aaatatgtga ctattattcc caaatagatg gacgtttaaa
      601 agtaattcat aagaaaaacg gtggaataat agatgcacgg aaaacaggag tcactagtgc
      661 tatgggagag tatatagttt ttctagatgg tgatgattgg cttgcaaata cttatttaga
      721 aaaatatgcg gaagtaatta ctaagtacaa tccagatata atatgtagtg ggtattttgc
      781 atcacgttcc aacaaaatta gaaaaagaga attgtcttgc tcgtttggat attatgacaa
      841 agataaaatt ataaatttga tttatcctaa attaattgag gatgaacatg gagagtattt
      901 tagaccacaa ttatgggcaa aagcttttag aactactctg tacaaggaac aacaacaagt
      961 atctgggcaa gttagtgttg gtgaagatca tgcatgtaca aaaccatgta tttaccatgc
     1021 aaattcgata tatatgatg
//
LOCUS       DACCXW010000281         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.292, whole genome shotgun sequence.
ACCESSION   DACCXW010000281 DACCXW010000000
VERSION     DACCXW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.292"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>1035)
                     /locus_tag="IVY71_003003"
     rRNA            complement(<1..>1035)
                     /locus_tag="IVY71_003003"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACCXW010000282         1013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.293, whole genome shotgun sequence.
ACCESSION   DACCXW010000282 DACCXW010000000
VERSION     DACCXW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.293"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            2..1012
                     /locus_tag="IVY71_003004"
     CDS             2..1012
                     /locus_tag="IVY71_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7986978.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT          420 a          167 c          179 g          247 t
ORIGIN      
        1 aatgaaaaaa aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc
       61 agcttgttca ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt
      121 atctgatttc tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat
      181 gatcatttat aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga
      241 tgcagaatac gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc
      301 tgctggatat acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc
      361 tggcttgaaa gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt
      421 ccatccagaa gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt
      481 gaaaaaatca gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga
      541 tacagagaca aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc
      601 tgcagaagta aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac
      661 tacaacaaat cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca
      721 aaataaaggc aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac
      781 taaactaagt gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa
      841 tgtaaaaatc aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc
      901 aagtacgaaa gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac
      961 tgattcaaca aaagaatctt caactgaaga aacaactgat tcatcaaaat aaa
//
LOCUS       DACCXW010000283         1002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.294, whole genome shotgun sequence.
ACCESSION   DACCXW010000283 DACCXW010000000
VERSION     DACCXW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.294"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            369..1002
                     /locus_tag="IVY71_003005"
                     /pseudo
     CDS             369..1002
                     /locus_tag="IVY71_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DACCXW010000284          985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.295, whole genome shotgun sequence.
ACCESSION   DACCXW010000284 DACCXW010000000
VERSION     DACCXW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.295"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            91..540
                     /locus_tag="IVY71_003006"
     CDS             91..540
                     /locus_tag="IVY71_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986979.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            540..>985
                     /locus_tag="IVY71_003007"
     CDS             540..>985
                     /locus_tag="IVY71_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986980.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRR"
BASE COUNT          358 a          156 c          222 g          249 t
ORIGIN      
        1 aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt
       61 acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc
      121 aatgtacagg tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg
      181 aagggagata aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt
      241 ggttcaaccg cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt
      301 ttaccaaagg gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca
      361 actacagcaa atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct
      421 ggagcacaga aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaacc
      481 gataaagtga aacttgatgg attagcgaat attacatttg agaaagtggg gacggtttaa
      541 tggctgatat tgttcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      601 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      661 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      721 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      781 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      841 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      901 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      961 acggagccat taaatggaga agaaa
//
LOCUS       DACCXW010000285          976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.296, whole genome shotgun sequence.
ACCESSION   DACCXW010000285 DACCXW010000000
VERSION     DACCXW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.296"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            147..>976
                     /locus_tag="IVY71_003008"
     CDS             147..>976
                     /locus_tag="IVY71_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7986981.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          342 a          143 c          167 g          324 t
ORIGIN      
        1 tctttagtga tataacgaaa tatctacaca gacaaaatga tagcgtaaaa tattttgtgt
       61 aaatagatat ttagggtaga aaaaaagaaa gtccattctg taaaattaaa gttaccacaa
      121 ccgaaatttt ataggaggac tttctcatgc ccaattttac tacagaaatt atggaaacac
      181 taatcaataa aggtgattta gatgaattat ttcgatgtca tttagaatta gctgttaatt
      241 cattattgca ggctgaatta acagcttttc ttgattatga aaaatatgat cgtgctgggt
      301 tcaattcagg taattcccgt aatgggaatt attcacgttc attcagaaca gagtatggag
      361 aattaaattt ggtaattcct agagatagaa atggtaagtt ttctcaacaa actttacctg
      421 cttataaaag aaccaatgac tctttagaaa ccactattat tcagctattt caaaaaggaa
      481 ttactatgtc tgaaatctct gaattgattg aaaaaatgta cgggcatcac tatacgccac
      541 aaaccatttc aaatatgact aaaatagttt ctgaagatat tattgccttt aaagaaaggt
      601 ccttagaatc cagatactct gtcattttta tggatgctac tcacattcct ttaaaaagac
      661 aaactgtttc aaaagaagca gtttatattg taattggcat tcgattagat ggaaccaaag
      721 aggttcttgg gtttagtctt gctccaactg aatctgctta tgtttggaaa gagattcttc
      781 aagacctaaa agaccgtggt ttaaaagagg ttttattagt cgtaactgat ggtttaagtg
      841 gcattaacga tagtattcat agtgtttatc caaatgctca atttcaacaa tgttgtgtcc
      901 atatatctag aaatatcgct cataaggttc gtgttagtga tcgacaagaa atctgtagcg
      961 atttcaaatt agttta
//
LOCUS       DACCXW010000286          971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.297, whole genome shotgun sequence.
ACCESSION   DACCXW010000286 DACCXW010000000
VERSION     DACCXW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.297"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IVY71_003009"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IVY71_003009"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IVY71_003010"
     tRNA            203..276
                     /locus_tag="IVY71_003010"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IVY71_003011"
     tRNA            316..405
                     /locus_tag="IVY71_003011"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IVY71_003012"
     tRNA            420..491
                     /locus_tag="IVY71_003012"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IVY71_003013"
     tRNA            507..579
                     /locus_tag="IVY71_003013"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IVY71_003014"
     tRNA            589..661
                     /locus_tag="IVY71_003014"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IVY71_003015"
     tRNA            697..772
                     /locus_tag="IVY71_003015"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IVY71_003016"
     tRNA            774..854
                     /locus_tag="IVY71_003016"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IVY71_003017"
     tRNA            861..934
                     /locus_tag="IVY71_003017"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
BASE COUNT          236 a          214 c          262 g          259 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg t
//
LOCUS       DACCXW010000287          910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.298, whole genome shotgun sequence.
ACCESSION   DACCXW010000287 DACCXW010000000
VERSION     DACCXW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.298"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..888
                     /locus_tag="IVY71_003018"
     CDS             <1..888
                     /locus_tag="IVY71_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP7986982.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          120 c          149 g          252 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc
//
LOCUS       DACCXW010000288          907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.299, whole genome shotgun sequence.
ACCESSION   DACCXW010000288 DACCXW010000000
VERSION     DACCXW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.299"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..214
                     /locus_tag="IVY71_003019"
     CDS             <1..214
                     /locus_tag="IVY71_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986983.1"
                     /translation="YCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRREKLP
                     EWAIDQEAYLKKKALERANRQSKAPF"
     gene            226..495
                     /locus_tag="IVY71_003020"
     CDS             226..495
                     /locus_tag="IVY71_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986984.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            658..>907
                     /locus_tag="IVY71_003021"
     CDS             658..>907
                     /locus_tag="IVY71_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986985.1"
                     /translation="MDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKTKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARS"
BASE COUNT          358 a          137 c          205 g          207 t
ORIGIN      
        1 gtattgtgaa tcatctggta agacttataa aaactatttg gcaactattc gaagttgggc
       61 aaggaaagaa aaaagtgaac ctaagaacgc aagcagtgga tacaagcgca caggaagacg
      121 agagaagctt cctgaatggg caatcgacca agaagcctat cttaagaaaa aagcgctaga
      181 acgagctaat agacaatcaa aagcaccatt ctaagaggtg gaaaattgaa gatcgattat
      241 ctagaactaa ttaatgaaat agcaaagtat aaaactggtg aggaaataga aattctgaga
      301 gacgtatatg atcaactcga agaagctgga attgaaggaa ttaagaatga tcgttcgagt
      361 tggagtaagc tcagatacta tttcgcactt tatatcgatg gaacacaatt aagaaattta
      421 gcatatacca aattactatt tgttgattgt gttaaaggct tgcaaaaaca tcttaatgaa
      481 cttgagcagg tgtaaacaag atggacctaa agacatttac agcacagatc gaattaatgc
      541 atcaagaagc tttaagacaa agtgtatcgt acgaagacaa gtggctcaac acgtttcatg
      601 gcggacgtga gagcgcactt gatcaagtac tcaaattatt gaaaggagaa tgtggggatg
      661 gataaaaaag cggcaatgaa acgaattgct gaattaacca agtcagaatc ttggcaagaa
      721 gacaaagaaa tagttgcaga agtccaaaaa ctcggcaaat caatgtggac tgaaaaaacc
      781 aaacggaaaa cgccgagaaa gattgcaatc tggcatggtg atcgaattct agtaacaggt
      841 actgctgaac agttatctga aattactgga ttaagcaaaa acattatttg ggatagagct
      901 aggagct
//
LOCUS       DACCXW010000289          904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.300, whole genome shotgun sequence.
ACCESSION   DACCXW010000289 DACCXW010000000
VERSION     DACCXW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.300"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            78..770
                     /locus_tag="IVY71_003022"
     CDS             78..770
                     /locus_tag="IVY71_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7986986.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          413 a          105 c          138 g          248 t
ORIGIN      
        1 gtgtctaaag aaagcttgac gaagaaaata atttttaata acttatttaa ggaaattttt
       61 tgaaggagaa gataaaagtg agaaaaaaga ttattattaa tgctggagta attataggat
      121 tgatgttagt aattttctcc ggaaagcaat atgctgataa tattttatta tggggagggg
      181 aagataatat tgaaaagatc aacaacaatt taactatatt agacacagct ctagaaaata
      241 aagagcaaaa aattagtatt ttaaattcac aattgtcttc aaacaaccaa actcttgaac
      301 aactaagaac taatattgaa gtttccaaac aaaaagtggc atctttagaa aacgacaaga
      361 atcaattagt atcagagaaa actaatttag aaaatcaatt gtcagcaaaa gactctgaat
      421 tgcaaggaaa acaaaatgaa attaatgcga aaatagacga aattaaccaa aaagtagctg
      481 aaataaatcg attaaatagc gattggaata atcgtttaca aaatgaaaag aacaaattta
      541 atgaagaaca atctactatt aatagtttaa accagcaaat taatcaaatg ttgagtgaaa
      601 aaaataacat tcaaaatcaa ctgcaagcta ctcagcaaaa gctagaagaa gaaaagtcaa
      661 aaaatagtag tttgaataat tacattgaaa agcttcaaaa agctaaaaat gatgtagagg
      721 ataccgcaaa taaagcacaa caaattgtaa atgaacatac aatccaatag atatagtttt
      781 aatttaacaa atggtatgtt tggagataat gagatagaat ataaatgtca taatattata
      841 caatctaaac accgtttcct tttctatttt tgatgacatc agtatatagc tactcgaaag
      901 agta
//
LOCUS       DACCXW010000290          897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.301, whole genome shotgun sequence.
ACCESSION   DACCXW010000290 DACCXW010000000
VERSION     DACCXW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.301"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            177..>897
                     /locus_tag="IVY71_003023"
     CDS             177..>897
                     /locus_tag="IVY71_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP7986987.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKY"
BASE COUNT          354 a          114 c          144 g          285 t
ORIGIN      
        1 aattatactg tttcctgata agctctcaat tcttctgaac ccatgatgtt agtgcttttc
       61 ttctattatt aaatatcttg gaattttcta ttctttctaa aaagtatggt caaagtatgg
      121 tcactattga tagaatgtta atagtagtag aaagctgata tctaggagga actaatgtga
      181 gtaaacaatg tgcaaagtgc ggaggtaaaa ttggcttaac ttcatataaa atcaaagata
      241 aacaaattgt ttgcggcaat tgtatgaaaa aagctggtta cggtatgact actccttata
      301 aaataataag atctttgaat ttagaagatt tagataagga tccatcagaa aaaagaaaat
      361 taaacatatc tgatcatact gcacaaaaca atcatgatat aggtaatgat agaatgagaa
      421 ccactgaaga gatggtagaa ttttgtctta aatacggcta cggtaaagga atgactaaaa
      481 aatggacaac tcatcatttt aacttaatat cagatcaact taacagtgac gaatacgtaa
      541 tcttctgctt tgtaggattg cataattata tctcatcaac taagcatgac aataattatg
      601 catacgcatt aactaataaa cgtttaattg tagctcaaca aaaaatgata ggcaataatg
      661 tacaaagtat tatattagac aatttaaata atattagtaa aaaaagagga gtgctgttgg
      721 gtacattaac tattgatact cttggtaaag tattgaatgt tgcagttgat aaagatactg
      781 ttgataggat aagtgattct ttaaacgaaa ttatttataa tttaaaaaga agaaatattt
      841 ctgctacaaa ttccaataca tcaacaattt cctcagcttc agaaataaga aaatata
//
LOCUS       DACCXW010000291          897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.302, whole genome shotgun sequence.
ACCESSION   DACCXW010000291 DACCXW010000000
VERSION     DACCXW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.302"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..288)
                     /locus_tag="IVY71_003024"
     CDS             complement(<1..288)
                     /locus_tag="IVY71_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7986988.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSES"
     gene            complement(288..>897)
                     /locus_tag="IVY71_003025"
     CDS             complement(288..>897)
                     /locus_tag="IVY71_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7986989.1"
                     /translation="EIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKT
                     NGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSV
                     DVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTI
                     IEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          300 a          156 c          100 g          341 t
ORIGIN      
        1 actctcagaa aaagcaggtt tttctaaaaa tatgtaatca actttttcaa caattacatt
       61 tagtgtttcc agatgcttaa aagtcggatt tgtaataaaa gctatatcat aatgtgtatc
      121 caaatcgtct ctattttgaa tatacgttat ttcttcaaac tctttttttc cactgctact
      181 ataagctgtt atatcaaaat taatagaatt cttttttcca tagcttatta gattttttag
      241 atgtctcttt ccaattgacc ctaacccaaa aaaaattact ttcatcatta atttacctca
      301 atgtcgttaa ttaagttaat aatatactta tctttttcaa aagtatattg gggttccatt
      361 ttcttttcca aaagcgtgaa aaattcttta atctcctcta aatatgcatc ttcaataata
      421 gtatgtgaat aattattatc atgcataacc tcatcgtaca attgaatatc tgtcatacta
      481 gaagtttctt cattccatga aaatagagaa tccgggttac cttcccattc tattaaaatc
      541 ttttcaccaa ttatagttaa atttctttta gctactcttg aaacaacatc tacacttaaa
      601 gttccaacta taccagtttc atgctcaata ataagagtga aactgtccga ataatcaata
      661 tcaagattgg ataatttctg ggtcattaca gtaaacgatt taactttccc aaatgtatct
      721 ataatccaag gtaattcaat agcgaatatt tctctacacc cattagtttt tttgtttgaa
      781 acaaaaaaat tgcgataatc ttcccaagga tgccagtcgg gtaaatactg tcctacatgg
      841 tattgataag aaattttttt attatgcgtc actttagact taatatattg aatttct
//
LOCUS       DACCXW010000292          892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.303, whole genome shotgun sequence.
ACCESSION   DACCXW010000292 DACCXW010000000
VERSION     DACCXW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.303"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..818
                     /locus_tag="IVY71_003026"
     CDS             <1..818
                     /locus_tag="IVY71_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP7986990.1"
                     /translation="QVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIVMNVLRKF
                     LTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKELDIS
                     MSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDI
                     EKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKL
                     TNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT          342 a          102 c          174 g          274 t
ORIGIN      
        1 atcaggttta ttttggcggg ggaattaatt tatggttagc caaaaagaat ggaatcagac
       61 agagaattgc tcatagccat gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg
      121 tattgcgtaa atttttaacc aaattgttgt tacaaaatgc aacagactac cttgccgtta
      181 gtcaagaagc tggtgaaagt ctgtttcaaa accacccctt tgagatagtt cataatggta
      241 ttgatttaga gctttatagt aaaaattcag aagctaaggt taataagaga aaagaattgg
      301 atatctcaat gagtactttt gttgtgggaa acattggacg attagagaaa caaaaaaatc
      361 aatcttattt gttagagatt tttttagaaa tactaaaaat gaattctgat tcttacttga
      421 ttttagtagg tgaagggtca cttagaaata aactgaaagc caaagcagta gaattggata
      481 tagagaaaag agtaaaattt ttaggagaaa gaaacgatat tcccgagtta ttatctgtga
      541 tggatgtttt tgtaatgcct tctctttatg aaggtctgcc aatggttgga ttagaagcac
      601 aagcagcaaa attaaaatta gtcttagcag atactatttc caaagataca aagttaacaa
      661 ataatgtgga ttttgtatca ttagatgaaa caccacaaga atgggcaaaa aaagtaataa
      721 aagagccgta tgctaataaa ataacacaag aactaaagag ttatgatgtt tcgtacacta
      781 ctgaacaaat ggagagtata tatagtagag atcattaaaa atgttcaaga tattttagga
      841 gtgattattt ttgaaaatat gttatttgat attggcatac aatgatattg gc
//
LOCUS       DACCXW010000293          882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.304, whole genome shotgun sequence.
ACCESSION   DACCXW010000293 DACCXW010000000
VERSION     DACCXW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.304"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            53..>882
                     /locus_tag="IVY71_003027"
     CDS             53..>882
                     /locus_tag="IVY71_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP7986991.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          305 a          132 c          160 g          285 t
ORIGIN      
        1 cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc atatgaacga
       61 ttttactaca gaaattatgg aaatactaat caataaaggt gatttggatg atttgtttcg
      121 tcgtcaccta gaattggcta ttaacgcttt attacaagct gaattaacgg tatttcttga
      181 ctatgaaaag tatgatagag ctggatttaa ttcaggtaat tcccgtaatg ggaattattc
      241 gcgctctttt aaaacagagt acggtgaatt gaatttagta atacctagag ataggaatgg
      301 agacttttct caacaaactt tacctgctta taaaagaacc aatgactctt tagaaaccac
      361 tattattcag ctagttcaaa aaggaattac tatgtccgaa atctctgatc taatcgaaaa
      421 aatgtatggt cattattaca aaccacaaac tatttcaaat atgagtaaaa ttgtgtctga
      481 agatgtttcg gcatttaaag aaagaacttt agaagctaaa tactcagtca tttttatgga
      541 tgctactcat attcccttaa aaagacaaac cgtatcaaaa gaagctgtct atatagtaat
      601 tggtatcagg ttggatggaa ctaaagaggt tctaggattt actatagctc caactgaatc
      661 tgcttatgtt tggaaagaga ttcttcaaga tctaaaagac cgtggtttag aagaggtttt
      721 gttagtcgta acagatggtt taagtggtat tgacgatagt attcatagtg tttatccaaa
      781 tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata aggttcgtgt
      841 tagtgatcga caagaaatct gtagcgattt caaattagtt ta
//
LOCUS       DACCXW010000294          871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.305, whole genome shotgun sequence.
ACCESSION   DACCXW010000294 DACCXW010000000
VERSION     DACCXW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.305"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(62..262)
                     /locus_tag="IVY71_003028"
     CDS             complement(62..262)
                     /locus_tag="IVY71_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7986992.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          282 a          149 c          136 g          304 t
ORIGIN      
        1 aattttttcg tacaaaaaaa ccgtcttttc tttcgaaaag acggttaaaa gtcaattttt
       61 attaagcttt aacaatgttt gttgcttgag ggccacgttg accatcttca acatcgtaag
      121 acactgcttg gccttcttct aaagttttga agccgtcacc ttggatagct gaaaaatgtg
      181 caaatacatc atttccatct tcaccagtga taaagccaaa acctttgtct gcgttgaacc
      241 attttactgt accgttattc ataaataaat tcctcctgat acgtatcaat gtacgttttt
      301 tcgttgcaat aaatatttga taggcaaaag gagttttatc tatgtagata tatcgctcaa
      361 attacgtttc aaaatagatt actcatttaa tatatcatgc tttttctaat aacacaagga
      421 taatgcaatg aaatataaaa ttcattgtct tctattgtct tcgaatcgaa gaaaaagttg
      481 aataaccgcg attgattcac tttttgtttt cttgtttatt tgcttttatc tgcaactttt
      541 gactttaaaa tttcttaact atgaaaacct ttcaattggt ggtatactta taaatggaat
      601 ttcgttatca acgcgttcgt tgcctttcag caagtattta aagaaaggag aatggctatg
      661 acattctata acaaagatat acaatatcga gtaaccttgg atacagattt gaatctattt
      721 gtcgtttttg acaaagaaaa taccaatcat gtagcgacag gtgttacgat cgaacaagca
      781 gtgcaagaat taaaaaaatc tgcataagga gtggaaaccc tcatatggac gattctcgtt
      841 cttctcttta aacgcatcta ctggtagata a
//
LOCUS       DACCXW010000295          871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.306, whole genome shotgun sequence.
ACCESSION   DACCXW010000295 DACCXW010000000
VERSION     DACCXW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.306"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>871
                     /locus_tag="IVY71_003029"
     CDS             <1..>871
                     /locus_tag="IVY71_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7986993.1"
                     /translation="SITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDT
                     HYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWI
                     LGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDN
                     PVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSV
                     SRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNV
                     DKGIYYYRTYEDSQ"
BASE COUNT          297 a          118 c          159 g          297 t
ORIGIN      
        1 tctattactt atgtaacaag tgatcattat tttggaagga attttgatta tgaaatatct
       61 tacaatgaag tagttactgt tactccaaga aattataagt tgaattttcg aaaggtaaat
      121 gatttggata ctcattatgc aatgattggt attgccgctg gtatagctga ctaccctctt
      181 tattacgatg cgacaaatga aaaaggattg agtatggctg ggctaaattt ttctgggtat
      241 gctgattata aagaaataca agaagggaaa gacaatgtat ctccttttga atttattcct
      301 tggattttag gacaatgctc aacagtagga gaagctaaaa aattgttaaa aaatatcaat
      361 ttagcaaata taaattatag tgacgaactt cctttatccc ctttacattg gctattagct
      421 gataaagaaa aatcaattgt cattgaaagt atgaaagatg gacttcatat atatgataac
      481 cctgtgggcg ttcttaccaa taatccttca tttgactatc aattatttaa tttaaacaat
      541 tatcgtgtct tatcgagtga aactcctaaa aataattttt caaatcaaat aagtttgaat
      601 gcctatagcc gcggtatggg agggataggc ttgcctggag atttatcctc agtatctcgt
      661 tttgttaaag cgacttttac gaagctgaat tctgtatctg gagattcaga gtcagaaagt
      721 attagtcaat ttttccatat cttaggttca gtagaacaac aaaaaggttt gtgtgatgta
      781 ggtgatggaa aatatgaata tacaatttat tcttcttgtt gcaatgttga caaaggaatc
      841 tattattatc gaacatatga agacagtcaa a
//
LOCUS       DACCXW010000296          847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.307, whole genome shotgun sequence.
ACCESSION   DACCXW010000296 DACCXW010000000
VERSION     DACCXW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.307"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            164..358
                     /locus_tag="IVY71_003030"
     CDS             164..358
                     /locus_tag="IVY71_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986994.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            355..801
                     /locus_tag="IVY71_003031"
     CDS             355..801
                     /locus_tag="IVY71_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7986995.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
BASE COUNT          331 a          180 c          172 g          164 t
ORIGIN      
        1 aaaaatcccg tgtatcaaca cgggattaga ttaagtttcc tcaatcacta ttcataacac
       61 aattattata acaagtaagt aaacaattga aaaggacatt tattgaaaac ggttgttttt
      121 tacttaagga ggaaatttag cgaaaaaatt agttgcattg tttttggtcg caggaagttt
      181 gttgagtggg tgtggtgcta ctacgtctac tactcaaagc acaagttcag atcaggcttc
      241 cagcagcagt accacgatct ccgaaagtac ggcagattca tcgaaagaac aagaacaaat
      301 tgaaaagaaa aaagaagaga aacaaaaagc ggaaaaagct gcactagaaa aaaagtgaca
      361 gaagcggaat ctgctttgtc acaagctgaa gcaaatccta caaaagaaaa ctatgcaact
      421 gcttcagcag ctatccaagc gctacctgaa aacaaacaag agttgacaag ccgtcttgct
      481 gcaattgatt ccacaatcaa agcaaaagaa gcagaagaag aacggaaaaa gcaagaagaa
      541 attgcacgac aacaagcaga acaagcgcaa caagaagcaa tcgcacagca acaagcagaa
      601 caggcgaggc aagaagcaat cgcacagcaa caagcagaac aggcgcgaca gcaatcagca
      661 gctgaacagg cctctcagca ggcacaagcc gcagctcctg agcaaaataa tgaacaaact
      721 gtctatgtca caccgaccgg ttcaaaatac catacacata aatgtggaaa tggtacgtat
      781 acccctgcca ctttaagcta agctcaagct cgtggtttaa ccccttgctc caaatgttat
      841 ggaaact
//
LOCUS       DACCXW010000297          846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.308, whole genome shotgun sequence.
ACCESSION   DACCXW010000297 DACCXW010000000
VERSION     DACCXW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.308"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..293)
                     /locus_tag="IVY71_003032"
     CDS             complement(<1..293)
                     /locus_tag="IVY71_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7986996.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRW"
     gene            complement(440..>846)
                     /locus_tag="IVY71_003033"
     CDS             complement(440..>846)
                     /locus_tag="IVY71_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP7986997.1"
                     /translation="TPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNA
                     ASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          273 a          165 c          141 g          267 t
ORIGIN      
        1 aaccagcggt aagcgacatt ggtatcgact tccttaatgg tttgtcgcat ggaacggata
       61 ccgaataaat actgaataaa caccaatttg atcagaatca ctggatcgat acttgggcga
      121 cctaaatcgg agtaagtggc ttcgacgatt ggataaataa attcaaaatc aagcgcggca
      181 tcaatctttc gaacgagatg atcttgagga actagatcat caatcgtaag catcgcaaat
      241 tgatttctac cattcgtatt gtttttagac atcatagcta tcactccttg cattcgtttt
      301 cataaatcta ttataaaaga aaagactgta gatatcatcc ataaaatgga ctttgtctac
      361 agtctgagac acgacctaaa gatcgtgtct aattatgact gtatcagaaa atatgatacc
      421 aaacggatct attcattaat taattgacat agtttatctg ttgctctttt atgactggat
      481 aactgaccaa ttcatgacga tccaagtctt ctttattcat atttacagca gtaatttgac
      541 tgtcttcata cgtacgataa taataaatcc ctttatcaac gttacagcaa gaagaataaa
      601 tcgtatattc gtattttcct tctccagtat cgcatactcc tttttgttgc tcgactgagc
      661 ctagtatatg aaaaaattga ctgatacttt cagattctga atcacctgat gcagcattca
      721 ttttagtaaa tgtggctttt acaaatcgag aaacagaaga gagatctcca ggtaagccca
      781 atcctcccat cccacgacta taaacattca agctgatctg gtttgaaaaa tgattttctg
      841 gtgttc
//
LOCUS       DACCXW010000298          804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.309, whole genome shotgun sequence.
ACCESSION   DACCXW010000298 DACCXW010000000
VERSION     DACCXW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.309"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..630
                     /locus_tag="IVY71_003034"
     CDS             <1..630
                     /locus_tag="IVY71_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7986998.1"
                     /translation="VELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPY
                     GYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQ
                     FGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNV
                     GYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          274 a          147 c          154 g          229 t
ORIGIN      
        1 gttgaattgg ctagagattc agcaatgaaa tatggtattc cattaacgtt ggacactcct
       61 tataaccaac cgggaatcaa atcgcattta tgggtaacac aaaacatctg gggcgatcat
      121 acagatcctt acggttatct ttctgaaatg ggcgtaagta aagaaaaatt agcatatgat
      181 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
      241 ccaacacgag ctggtgcagc taatcctaca ctgacagatg gaacgaatta cgcccacatt
      301 gatcagtttg gagaaatcga aaatgcaaat ttgcatgtag ctggatggca cattgctaac
      361 tataaatacg agtatatctt cattatggat tacaatactg ggaaagaatt agctcgagta
      421 agagctgatg gaatttatag agcagatgta aatcaagctt ataatacttc tggaaatgtt
      481 ggctatcatg tatcttttaa catgcgtaac ttccctaata agaaagtcta tgtcatgatg
      541 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
      601 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcattacata
      661 actatattga caactataaa aatcattcga taaaatagtg atgttatcgc atatcttcac
      721 tatcacccat aatagtcaca ctccaagcta tgcgataaca ggtttgttgc cacacattct
      781 actggttgat tgtttatggc ttta
//
LOCUS       DACCXW010000299          799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.310, whole genome shotgun sequence.
ACCESSION   DACCXW010000299 DACCXW010000000
VERSION     DACCXW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.310"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..652
                     /locus_tag="IVY71_003035"
     CDS             <1..652
                     /locus_tag="IVY71_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7986999.1"
                     /translation="KILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIV
                     FEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKI
                     EHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRK
                     ISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          285 a           91 c          148 g          275 t
ORIGIN      
        1 taaaatactg gaatatgtta aaaatggaga tattcgaaat ttagaaaaca tggtttttaa
       61 tctgagtaat ggaataattc caagtgttag tggagatacg attcggtctg aaaaaaatta
      121 ttctatcatt gtttttgaaa agttagcaca aacgtctata acattaggaa tggatataat
      181 agaagcgtat caaagtagag atgctttgat acaagaaaac gaattagctg tatcgttacc
      241 tgaggtctta aaagtgaggg attctgggat tgtttattat actaaggaaa tcggaaaaac
      301 taagatagag catctttctc ccttaatttc ttctgtcgtt caattcatcg gcttaaatat
      361 ctataaaaga attactgtaa aggaaatagc taattatttt tctgtaagtg aaactaaatt
      421 aagagaaagc tttaagaatg aaatgcatat tacgatttat aattatatct ctaagcgaaa
      481 aatttctacg gctaaaataa tgttgaaatc aaaccatact atcagtgaag tttctttagg
      541 tttaggattt tcagattctt cacacttttc gagagtattc aaaaagtacg caggtgtatc
      601 tcctaagcaa tatcaattag gtttagtgga caatttcaac aacattagct gagaggtttt
      661 attttattat gagactatat catttacgta gtttggagga gttgaggaaa ttggatttta
      721 acaaaaaatt tttttgggaa atttctgatt tgagttgttt cgtagaatct gagcgtctaa
      781 gtaatggtaa taatagtgt
//
LOCUS       DACCXW010000300          796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.311, whole genome shotgun sequence.
ACCESSION   DACCXW010000300 DACCXW010000000
VERSION     DACCXW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.311"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          265 a          129 c          100 g          302 t
ORIGIN      
        1 acaataaata actttagtta ctttaatgcg ttcttacttt atttttatat tatcattttt
       61 cttattcact tctttggttt atactattct tataaataaa aatgaggtat gtcctatgat
      121 gaaaagattg tactattcat tgatcataac gattggctat ttgattgtat caaatctagg
      181 aaatatggta tttggtatca gtaaagaatt cagttggact actacattat gggaatctct
      241 attctttttc atcttcgtat ttttgttaca aaattatcga aaaaaataaa catgtgccat
      301 ctatctgttg gcacatgttt taggatttat ggtacttttt attcggcaat ccattttatt
      361 tttctacttc ttcttaatac ctctaacact tttgatttat aaactaatcc aatgcatcta
      421 cttgcgctta ttaagctatt tcagctagat agtaccaccc catgcgtcaa tgcttgattt
      481 tccttttgta taacattgta taagcaataa aggacaaata agtggtgatt acaccaaacc
      541 attgaataag aaaaattctg atacgtaaat aaacatgaac ttgaaaaaat taatgaatcg
      601 gttagctact acgtagttgt cttttttctc atacagttct gttaacttac acttctcttc
      661 gctagataat aaaaaatcct ccaactgtat tgtccaatat aatcccaggc atctaaacta
      721 gcattataga agagacacac atagaatcta aaataactgg ttttaaatgt ttcttacaat
      781 caaaaaaagc tgttaa
//
LOCUS       DACCXW010000301          783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.312, whole genome shotgun sequence.
ACCESSION   DACCXW010000301 DACCXW010000000
VERSION     DACCXW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.312"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>783
                     /locus_tag="IVY71_003036"
     rRNA            <1..>783
                     /locus_tag="IVY71_003036"
                     /product="23S ribosomal RNA"
BASE COUNT          242 a          152 c          233 g          156 t
ORIGIN      
        1 gggggaaccc aacatcttta ataggatgtt acgattgtgt gaatacatag cacattcgag
       61 gtagacgcag agaactgaaa catctaagta cctgcaggaa gagaaagaaa attcgattcc
      121 ctgagtagcg gcgagcgaaa cgggaaaagc ccaaaccagc aagcttgctt gttggggttg
      181 taggactcca atatggtagt tctttcagat agtcgaatga cttggaaaag tcagtcaaag
      241 agggtaaaaa ccccgtagac gaaatgtgga agacacctag gaggatcctg agtacggcgg
      301 aacacgagaa attccgtcgg aatccgggag gaccatctcc caaggctaaa tactccctag
      361 tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa gcaccccgga aggggagtga
      421 aatagaacct gaaaccgtgt gcctacaaca agtcaaagcc cgttaatggg tgatggcgtg
      481 ccttttgtag aatgaaccgg cgagttacga ttgcatgcga ggttaagttg aagagacgga
      541 gccgcagcga aagcgagtct gaatagggcg tttgagtatg tagtcgtaga cccgaaacca
      601 tgtgatctac ccatgtccag gttgaaggtg cggtaaaacg cactggagga ccgaacccac
      661 gtacgttgaa aagtgcgggg atgaggtgtg ggtagcggag aaattccaaa cgaacttgga
      721 gatagctggt tctctccgaa atagctttag ggctagcctc ggaattgaga atgatggagg
      781 tag
//
LOCUS       DACCXW010000302          776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.313, whole genome shotgun sequence.
ACCESSION   DACCXW010000302 DACCXW010000000
VERSION     DACCXW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.313"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..210)
                     /locus_tag="IVY71_003037"
     CDS             complement(<1..210)
                     /locus_tag="IVY71_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HAP7987000.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSW"
     gene            complement(203..>776)
                     /locus_tag="IVY71_003038"
     CDS             complement(203..>776)
                     /locus_tag="IVY71_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7987001.1"
                     /translation="EFYYKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPP
                     KIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFN
                     SSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEK
                     LFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          271 a          121 c           88 g          296 t
ORIGIN      
        1 ccaagaaaaa cgcattttaa aaaaattttt ctttataaaa acctcataca aaactataat
       61 tccaccccaa gcaaaagcta ttttcatcgg gatattagtt acattaaaag tactaggcat
      121 aaaataaagc agacaaatga ttaaatttat tactcgaaac gcatctcgac ttaataaact
      181 cttctctaaa acaggtacta ttctattcat ctatatttcc aactttcaaa atttctttat
      241 caattctatt taatagttta tcagagtaat ccgatttaac ttttcttaca aataattttt
      301 cactttcttt tatttgatct atatcccttt catcaaacca tttgttcatg gaaggatcaa
      361 taatatgaat atttgctaat ttcgaagttt gtaaagtagt ttcttcaata tttttcaatt
      421 caagtggtaa atctggtcct ccaataacat ttttattttt gaacgaggaa ttaaaaaaaa
      481 gtgtatgaaa aaatttttca tctggggcaa acatacctat tttaaaattc aggtagtctt
      541 gttgattttg ttcatacatt tgtattaatt ctgttaggca ttctttcgac aaagcccacc
      601 attgagaacc atgatacaaa tcaaatttat ggtgtctaat ttttggggga agtttaatca
      661 tcctcagaca acgattaatt atagctctaa aaattttaaa tgcagttgtg ttattcctat
      721 taattctagg ataatcatgt ttttgatata catctatatg tttataataa aattct
//
LOCUS       DACCXW010000303          754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.315, whole genome shotgun sequence.
ACCESSION   DACCXW010000303 DACCXW010000000
VERSION     DACCXW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.315"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..222
                     /locus_tag="IVY71_003039"
     CDS             <1..222
                     /locus_tag="IVY71_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7987002.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            403..>754
                     /locus_tag="IVY71_003040"
     CDS             403..>754
                     /locus_tag="IVY71_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7987003.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIF"
BASE COUNT          278 a          134 c          135 g          207 t
ORIGIN      
        1 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
       61 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
      121 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
      181 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
      241 gattttattc tttatttaaa actttgcaac agaaccaccc gcactatgcc attacattta
      301 tatctacgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa
      361 attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt
      421 accatagacg aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa
      481 atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag
      541 caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt
      601 agaaaagtca ttcaattacc tgctcatgaa gtagattaca ttaaagagaa agtcactctt
      661 ggttggacgc ctgacgtcat tatcgggcga aaagaaaggt ctgtttcatg cggtatgaga
      721 acactttatc gtttattttc taaaggaata tttg
//
LOCUS       DACCXW010000304          752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.316, whole genome shotgun sequence.
ACCESSION   DACCXW010000304 DACCXW010000000
VERSION     DACCXW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.316"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>752)
                     /locus_tag="IVY71_003041"
     CDS             complement(<1..>752)
                     /locus_tag="IVY71_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7987004.1"
                     /translation="YNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRA
                     IDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVS
                     VFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKE
                     LSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFC
                     STNLFPWQLFNHTPVKSLQFVWRLNGFSSLFI"
BASE COUNT          320 a          114 c          118 g          200 t
ORIGIN      
        1 ctataaataa agaagaaaaa ccatttaacc gccagacaaa ttgtagactc ttaactggtg
       61 tatggttaaa aagttgccaa ggaaataaat tggttgaaca aaaaagaaca aatacaccaa
      121 gaaagaaaat aaataaatca tcagcagtta atttttttat aaaaataaaa gcaccaatca
      181 atccgagaaa taagaataat ccaatagtat agttgttata gttattattt aaaatatgtg
      241 tcagcatatc actaggagcc ataccactta attcttttcc cagtgggact ttcaattctt
      301 gtgctctaga ttgttgaata aaaggaatta agaatcccgc acttaaaaaa aaggtgagga
      361 cggtagcttt taaaagactt aagagtctag cgattttttg gtcccaaaaa taaaaagaaa
      421 gaaacaacgt tcctccaata aatacagaaa ccatagcgac agaaagtaaa tgagtataca
      481 ctactagcgt catgccaata gacaaccagt accatttttg gtaatctcta atataaattt
      541 cgtaacaccc cattagtatc aaaggcaaaa atgttaaagc gacagcttct cccaatgaag
      601 cacgatggaa tatgtcaatg gcacgatagg cggaaaatgt ataaataatc gaaaataaca
      661 gcgaaatata acgattgttt tttatttgtt tcatagaaaa ataagaaacg accattgtta
      721 aaaaagtgat aaaaaaatag tagatattat aa
//
LOCUS       DACCXW010000305          749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.317, whole genome shotgun sequence.
ACCESSION   DACCXW010000305 DACCXW010000000
VERSION     DACCXW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.317"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>749
                     /locus_tag="IVY71_003042"
     CDS             <1..>749
                     /locus_tag="IVY71_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7987005.1"
                     /translation="IKPNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYR
                     LAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFL
                     FIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGY
                     QSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLC
                     ERKNSCSLYNSMNKFLKLSYEQRINMGKMGR"
BASE COUNT          285 a           84 c          153 g          227 t
ORIGIN      
        1 atcaaaccaa atatatacgg tggattggca tgtcggttaa gaaaggtttc ttatgcatta
       61 aatataaccg gattaggaac ggcatttcaa aaaaaggggg ttttgaaaag tcttattgta
      121 tttttgtata gattagcagc taaaaaatct aaggtcatat ttttcgaaaa ttttgaaaat
      181 atgaaaacat ttcagacttt acaaattgct aaacaaagta aatacaagct attaaacggt
      241 gcaggagtag atgtagaaca ctttagttat ttagaatatc ctaaagatga gcaagttcat
      301 tttttattta ttggaagaat tatgaaagag aaaggtgtag atgaactttt tgaagcaatg
      361 aaaaacttat atagtgaaga gaaaaattgt gtacttgatg tccttgggta ttttgaagaa
      421 gattacggca taaaaattga aaaatacgaa aaagagggat ggttaaaata tcatggatat
      481 caatctgata ttcgtccctt tgtagaaaga gcacattgct ttgttttgcc tagttggcac
      541 gaaggaatgg caaatactaa ccttgaatca gcagcatcag ggagaccaat tattacaagt
      601 gatatcccag ggtgtaaaga agcagttatt gaatcgaagt ctggttattt atgtgaaaga
      661 aaaaatagtt gtagtctcta taattcgatg aataaatttc taaaattatc atatgagcag
      721 cgcattaaca tgggaaaaat ggggagaaa
//
LOCUS       DACCXW010000306          734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.318, whole genome shotgun sequence.
ACCESSION   DACCXW010000306 DACCXW010000000
VERSION     DACCXW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.318"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          238 a          113 c          111 g          272 t
ORIGIN      
        1 actcaaaata aaaaaaacct tacattatac tggaatgaac tgtattgata gtgtacctaa
       61 aagctctact gacataagat cgtgtaatcg gtaaagtaat tttattaatt atacgggatg
      121 taatcaatta gagaataaac taatcataaa aggtactttt acattcataa ttgtcgcttt
      181 gagattctac tttggttcac tatctgaccc aattgcacat tttcgatcat tacaagtatt
      241 ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt tttcagaatc tatgctaatt
      301 tctgtatgga tgttccagtg atggcaaatt tctcttttca ttgtttttct tgtcgcatga
      361 tatgcctttt tgtgttattc agttaaacta tagaaattct ttcctagaca gatcttgatc
      421 ccaaatttta cgtttttgag catcgatata gtttaaaatg acattactca tatctaaaga
      481 gtttagattt tttaggacta aaactgtcag ttgttctttt tcttctttct gttaaagtat
      541 tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa
      601 aaaacgaatg acttgaggaa atcacctcaa tcattcgttt aaaatgctat atattcttta
      661 gtttaagata cggaaattaa tgccatcgac cacactagta gaatgaacga catttacttg
      721 gccttttttt aatt
//
LOCUS       DACCXW010000307          702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.319, whole genome shotgun sequence.
ACCESSION   DACCXW010000307 DACCXW010000000
VERSION     DACCXW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.319"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            6..476
                     /locus_tag="IVY71_003043"
     CDS             6..476
                     /locus_tag="IVY71_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7987006.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(529..644)
                     /gene="rrf"
                     /locus_tag="IVY71_003044"
     rRNA            complement(529..644)
                     /gene="rrf"
                     /locus_tag="IVY71_003044"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          227 a          143 c          114 g          218 t
ORIGIN      
        1 actttatgtc tatttataat tttgaaacta ctttagaaga cggcactgtc tattcattga
       61 aaaaatatgc aggcaaaccg ataatcattg ttaacactgc aacaaaatgc ggtctagcac
      121 ctcaatttaa acaattagaa gaactttacc aagcttatca gacacaaggt ctggttatct
      181 taggctttcc ttccaatcaa tttaaacaag agctagattc agcgactgaa gcagcagaag
      241 cttgtaaaac cacttatggc gttaccttcc ctatgcatca attgattgac gtaaatggag
      301 aaaatacaga tcctcttttt acttatctta aaaatgaagc tcctggtact gtcactaaag
      361 caataaaatg gaattttact aaatttttga ttgatagaaa tggcaaagta atcgaacgat
      421 ttgcaccaca aacttctcca ttgaaaatga ccgctgcaat tgaatccgtt ctttaactta
      481 ataaaaataa aaaaagatga actatttcta gttcatcttt ttgcctttgc gtggcgacgt
      541 cctactctca caaggggcaa cccctcacta caatcggcgc taagaagctt aacttctgtg
      601 ttcggcatgg ttacaggtgt atccttctcg ctatcgccac cacactgtgg tgttatcttt
      661 tattgagtaa attttgttca ctcaaaactg gatttgaagt ca
//
LOCUS       DACCXW010000308          700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.320, whole genome shotgun sequence.
ACCESSION   DACCXW010000308 DACCXW010000000
VERSION     DACCXW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.320"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>700
                     /locus_tag="IVY71_003045"
     CDS             <1..>700
                     /locus_tag="IVY71_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7987007.1"
                     /translation="IKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVKGEDI
                     KKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDST
                     KETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKG
                     ADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFF
                     VKGDKNLDSVIKDLG"
BASE COUNT          303 a           80 c          134 g          183 t
ORIGIN      
        1 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
       61 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
      121 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
      181 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
      241 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
      301 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
      361 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
      421 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
      481 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
      541 gagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
      601 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
      661 aaggtgataa gaatcttgat agtgtaataa aagatcttgg
//
LOCUS       DACCXW010000309          692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.321, whole genome shotgun sequence.
ACCESSION   DACCXW010000309 DACCXW010000000
VERSION     DACCXW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.321"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            3..623
                     /locus_tag="IVY71_003046"
                     /pseudo
     CDS             3..623
                     /locus_tag="IVY71_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288785.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          248 a          121 c          126 g          197 t
ORIGIN      
        1 gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat gaacaacgtc
       61 aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa caatctggag
      121 cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga gagcaagata
      181 tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt atcgattcag
      241 tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg atcatggctg
      301 aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt taaatctcag
      361 ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa ggaattaaaa
      421 tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca gacaccaaag
      481 atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat ggggttgcta
      541 tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga ataaaaaaag
      601 aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta attagctttg
      661 tcatcaatac agacaatcat tttagattca tc
//
LOCUS       DACCXW010000310          686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.322, whole genome shotgun sequence.
ACCESSION   DACCXW010000310 DACCXW010000000
VERSION     DACCXW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.322"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(46..267)
                     /locus_tag="IVY71_003047"
     CDS             complement(46..267)
                     /locus_tag="IVY71_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987008.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(365..610)
                     /locus_tag="IVY71_003048"
     CDS             complement(365..610)
                     /locus_tag="IVY71_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987009.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          211 a          135 c           92 g          248 t
ORIGIN      
        1 tacatatagt aaccaaagag ttaataaaat aaaaagagcg aaccattata gatcagctct
       61 tttttcattg ctgtttttaa tcttatctag aatcacttca aatgtttcaa aggtatttac
      121 atctaactga cataaccaaa gcaagtactt cttttctttg taagtccaat caatattacg
      181 gtaagaggac attagattat ggtaccttgc tctttgttca actgaagcca attcccagcg
      241 tttttcaaat attttttctt cttccattat tgatccctcg caagacgcta cctcaaactt
      301 aaataatggg cagcgaaagt ctaattcctt agatctaagt atagcaaaca aatatgaaaa
      361 aaacttagaa ggtagtaaaa gttcttccgt cttttgcttt gcttctattc ttaatgtgtt
      421 tcgttatcgg tgccctacaa agattttcat taattacaca ctcatttatt gttgtgtatt
      481 ttctgacatt tctctcttga tcaattattt taattccatg ggcatcctga tagttcatag
      541 gagaggagcg gctatcagaa atagttgcac ctatctcttg aacttctttt acaacttctg
      601 gaaaatccat ccatcctgct tcagctgtca aaagtaataa tctataatat ttatctttcc
      661 ttttcaatct aagaaatcgt cacccc
//
LOCUS       DACCXW010000311          683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.323, whole genome shotgun sequence.
ACCESSION   DACCXW010000311 DACCXW010000000
VERSION     DACCXW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.323"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..158
                     /locus_tag="IVY71_003049"
     CDS             <1..158
                     /locus_tag="IVY71_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7987010.1"
                     /translation="FEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEV
                     LKIAEEK"
     gene            159..>683
                     /locus_tag="IVY71_003050"
     CDS             159..>683
                     /locus_tag="IVY71_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP7987011.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQT"
BASE COUNT          265 a           82 c          126 g          210 t
ORIGIN      
        1 gttttgaaga aaacattgtt aaagaaatgc ctgaatctgc taatgatatg tatgagaagg
       61 agttgaaata tttctttgaa aacgtagtaa caggcaaaga aaactggaat gatttatatc
      121 atgcaaacga agtattgaaa atagctgagg agaaataaat ggaaatttta tttactattt
      181 gtggtagagc tgggtctaag gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc
      241 ccctagtcca tttcgcttta gcaatgatag atatctatca acaaaaacat cctgaacata
      301 attgcacgac ttgtttgaat acggacagta atgagttagt agaattgacg actaagttaa
      361 atccgtctgt gaaaattgta gaacgtaaac ctgaattagc tacagatact gtagggaaaa
      421 ttgaagtgat acgtgattca ttcttacaac tagaaagaga agataatata tttgatttaa
      481 ttatagattt agatttgact tcacctttac gtgaagtttc taatcttgaa gaattaatag
      541 atgaacatct aaaaaattta gataaagacg tctacttttc agttgtttct gcaagacgta
      601 atccctattt taatatggta aaaagaattg aaaatgaagt tactatagtt aataaatcaa
      661 gatacacatc taggcaacag aca
//
LOCUS       DACCXW010000312          672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.324, whole genome shotgun sequence.
ACCESSION   DACCXW010000312 DACCXW010000000
VERSION     DACCXW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.324"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            33..656
                     /locus_tag="IVY71_003051"
     CDS             33..656
                     /locus_tag="IVY71_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987012.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          238 a          104 c          144 g          186 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat ga
//
LOCUS       DACCXW010000313          641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.325, whole genome shotgun sequence.
ACCESSION   DACCXW010000313 DACCXW010000000
VERSION     DACCXW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.325"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            4..546
                     /locus_tag="IVY71_003052"
     CDS             4..546
                     /locus_tag="IVY71_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7987013.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
BASE COUNT          205 a          105 c          128 g          203 t
ORIGIN      
        1 caaatgattg atccaaaaat gttggggatg atcttcctca ttaattttgc ttatatcact
       61 ctgaacacgt tacgtttcat gttgacaatg aaaggctatc gactgattgc tccacttgtc
      121 agtatggtcg agatcacgat ttatatcgtc ggcttatccc ttgttttaga tcgtttagat
      181 aatccactga atttatttgt ctatgcgtta ggttacgcag taggtatcag tgtcggaatt
      241 ttgattgaag acaaattggc attaggatac atcatggtca caacgatttt gccagcaaat
      301 agcgcagaag ataaaagttt gccgggtata ctgagagaac acggttatgg tgtgacacag
      361 acttctgcaa tggggctgga aggtgagcgg ctagttctcg aaatactttc tcctagaaaa
      421 agcgaacgtg aactgtatcg cctgatcaaa gaaatcgaag agcgtgcttt tatcatttct
      481 tatgagccaa aatatatttc aggtggtttc tggactaaaa aagtgagaaa acgacaagga
      541 aaataactca caaacttaaa aactgtctaa tatctaaatt aaattttaaa tgttagacag
      601 tttttattta ttattttgat taaaaaacat cgacatatac t
//
LOCUS       DACCXW010000314          637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.326, whole genome shotgun sequence.
ACCESSION   DACCXW010000314 DACCXW010000000
VERSION     DACCXW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.326"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..446)
                     /locus_tag="IVY71_003053"
     CDS             complement(<1..446)
                     /locus_tag="IVY71_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7987014.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          187 a          115 c           88 g          247 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattag
//
LOCUS       DACCXW010000315          615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.327, whole genome shotgun sequence.
ACCESSION   DACCXW010000315 DACCXW010000000
VERSION     DACCXW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.327"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(13..86)
                     /locus_tag="IVY71_003054"
     tRNA            complement(13..86)
                     /locus_tag="IVY71_003054"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Ile,seq:cat)
BASE COUNT          216 a           97 c          108 g          194 t
ORIGIN      
        1 tggctacatt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
       61 gctctgacca actgagctaa gggtagtggt tgttgccaca taaagccata aacaatcaac
      121 cagtagaatg cgtggcaaca aacctgttat cgcatatctt ggagtgtgac tatttatgag
      181 tgatagtgaa gatatgcgtc tccatatatg tatggttgag acagataaac atgtatgaaa
      241 aacgttgtta aatcaacgtt tttttatttt ggtcaaggag agaaaagtag agaaatacaa
      301 aattttttgc attgattttg cattcaaaaa aacattcaaa catttagaaa tccccaatac
      361 gtattcagga aaatctattt taaggtttac aaaaagaact tttgttcgta cactcttttt
      421 gaggtgaact tttatgctga tggaaggaaa aacacaatta tggtttaagc atgttccttc
      481 aaataagttt tttaattata aactctagag aaattaattc atcctaacat aaaataattg
      541 ttcactcaaa agtttttcta gaacaattaa aacagcaggc ttcacattcg ctatacattt
      601 gtaagaagta ataat
//
LOCUS       DACCXW010000316          599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.328, whole genome shotgun sequence.
ACCESSION   DACCXW010000316 DACCXW010000000
VERSION     DACCXW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.328"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>599
                     /locus_tag="IVY71_003055"
     CDS             <1..>599
                     /locus_tag="IVY71_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987015.1"
                     /translation="DWLKIEKGNTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVE
                     DNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDE
                     LDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSSGGNDSTILTI
                     PEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRR"
BASE COUNT          216 a          104 c          127 g          152 t
ORIGIN      
        1 gactggctca agatcgaaaa aggcaacaca cgaactccga atattagtga gtataaatat
       61 cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt atgtttggga tctagcacca
      121 gaatatgtcg aagataactt ggccacagat attaaaattt ctgaaattac tggtaaagca
      181 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      241 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      301 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      361 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      421 tattttgtgg ctttaaacgg aaaaagcagt ggtggaaatg attcaacgat actaacaatt
      481 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      541 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaa
//
LOCUS       DACCXW010000317          596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.329, whole genome shotgun sequence.
ACCESSION   DACCXW010000317 DACCXW010000000
VERSION     DACCXW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.329"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            68..514
                     /locus_tag="IVY71_003056"
     CDS             68..514
                     /locus_tag="IVY71_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7987016.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          201 a           85 c          123 g          187 t
ORIGIN      
        1 atgttaagtg gattttaaaa tgtaaaaata gtatgataag acgagaataa caacgtgggg
       61 tgtttacatg gaaaataagt tagtcgaaga ggttatcgaa aagttaaaaa aagagaacat
      121 tcgaatcaca cctcaacggt acgcggtttt agaatatttg attgaacatc attcccatcc
      181 aacggctgat gagatttata aagcacttga acatcggttt ccaaatatga gtgttgcgac
      241 tgtttacaat aaccttcgct tatttacttc gattggattt gttcaagaaa tgaagtacgg
      301 cgatgcttct agccgatttg atttcagttc aaagcgtcat tatcatgtaa tttgtgaaaa
      361 ctgcggtaag attgttgatt ttcattaccc agggcttgaa gatatagaaa tggctgcaag
      421 cagactgact gattttgaga ttaatgaaca tcggttggaa ttgtatggac tctgtccgga
      481 ttgtaaagag tcagcaaaaa aggcgcaaat gtaaaaacag tttctacaag tgttatacct
      541 aaaaaaagct agctgtgtaa aaaaaacgct agctttttta tttttttgtc ttttat
//
LOCUS       DACCXW010000318          595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.330, whole genome shotgun sequence.
ACCESSION   DACCXW010000318 DACCXW010000000
VERSION     DACCXW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.330"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..450)
                     /locus_tag="IVY71_003057"
     CDS             complement(<1..450)
                     /locus_tag="IVY71_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7987017.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          171 a          105 c           73 g          246 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaa
//
LOCUS       DACCXW010000319          588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.331, whole genome shotgun sequence.
ACCESSION   DACCXW010000319 DACCXW010000000
VERSION     DACCXW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.331"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..483
                     /locus_tag="IVY71_003058"
     CDS             <1..483
                     /locus_tag="IVY71_003058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987018.1"
                     /translation="KKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGII
                     DESDLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKV
                     FNCYKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK
                     "
BASE COUNT          239 a           66 c          107 g          176 t
ORIGIN      
        1 aagaaaagta attatgtatc tgtaggaaaa atattagaag ttgctagtat taaacaattt
       61 ataagccaag ctgataaatt aaaagagaaa cttaggataa ttattttact ttcattaaat
      121 agaggcatca ttgatgaaag cgatttgaat agttttatta aagatattga agctaaaggt
      181 gaaaagcttt cagtggaaga agtagaaaaa cgagctgtaa aacataattt ggtttgcgag
      241 acaagggaac aagtaattaa agaaataaaa tcattatctg aagatatggt aaaatatcag
      301 aaggtgttta attgctacaa gaaatataca caagaatcct atgtaatgcc acttaatatc
      361 caaacgatta gtaaaaatag aattagaaga attaacaagt ttgatccttc tggaaatatg
      421 aaggcacctg gaaatgtcct caatgaagta gatgaaagtt taaaaaaata ttttacaaag
      481 taaacgatat ttttgttgac atttttctgt tcgttagtta ccatgtagtt atagaaggac
      541 gtaagcgcct atttttaatg tacacatcgg gacgtatgcg cccacata
//
LOCUS       DACCXW010000320          573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.332, whole genome shotgun sequence.
ACCESSION   DACCXW010000320 DACCXW010000000
VERSION     DACCXW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.332"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..505
                     /locus_tag="IVY71_003059"
     CDS             <1..505
                     /locus_tag="IVY71_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987019.1"
                     /translation="IQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFS
                     GKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMM
                     FEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRK
                     LVEYGII"
BASE COUNT          203 a           79 c           90 g          201 t
ORIGIN      
        1 catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta tcttttttga
       61 tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg ctaaagtaaa
      121 tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa
      181 tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga
      241 aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc
      301 tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt taaatgaata
      361 tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt tctgtgaaca
      421 ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag aattaagaaa
      481 gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt aagtccagtt
      541 tctggtatac tatagggaat ttttttatta agg
//
LOCUS       DACCXW010000321          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.333, whole genome shotgun sequence.
ACCESSION   DACCXW010000321 DACCXW010000000
VERSION     DACCXW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.333"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>534
                     /locus_tag="IVY71_003060"
     CDS             <1..>534
                     /locus_tag="IVY71_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7987020.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DACCXW010000322          531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.334, whole genome shotgun sequence.
ACCESSION   DACCXW010000322 DACCXW010000000
VERSION     DACCXW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.334"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..184)
                     /locus_tag="IVY71_003061"
     CDS             complement(<1..184)
                     /locus_tag="IVY71_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987021.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKW"
BASE COUNT          179 a           97 c           52 g          203 t
ORIGIN      
        1 gccatttttt gattttcact aattgattgc tcataaatct ttcactggac ttttgttcaa
       61 tcttaaattg caaaacttct aactcataat tcccatcttc tgatttattt ttgggaacaa
      121 ctatcaaact tttataaagt ttatccacat caaaatttat cttttttgca taatttgtag
      181 ccattaatac atctccttat tcataataaa tttcatatga cgctcctctc aaaatttacc
      241 ggtagactag aaaaaagaat aaaagatacg tcaattcaca ctttattctt aaaaaaaaac
      301 tggtctctct accggtaata tcgattgagt ccgcctttat cgactttatc ctttttttat
      361 attaaatttt ttcaccggta gacagggaga gaaagaataa actaaaaagc ccttaattct
      421 taatgttttc acctccttac cgttcctacc gctaaaattt aatataacta aattctataa
      481 cattagaaga aattttaaat acctttgata aaaaatattg atgttttttc t
//
LOCUS       DACCXW010000323          519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.335, whole genome shotgun sequence.
ACCESSION   DACCXW010000323 DACCXW010000000
VERSION     DACCXW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.335"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>519
                     /locus_tag="IVY71_003062"
     CDS             <1..>519
                     /locus_tag="IVY71_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987022.1"
                     /translation="NLVNRKSTTKFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIE
                     REHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDEL
                     EILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAP
                     QIEGEDKEKRID"
BASE COUNT          214 a           56 c           96 g          153 t
ORIGIN      
        1 taacttagtt aatcgaaaaa gtacgactaa atttgcaaac tcattctggt ttttaaaaaa
       61 tattaataaa acagtggaaa agaatggagt ttataaaggt gattttcatt ttcaaaatgc
      121 tgagactatt gaacgagaac atcattattt cgatttatta gtgaatacag tggtagaatt
      181 agcgaaatgg gcaaagtcaa acgaaactta tagtcaagta cttaaaagaa ttatgagtca
      241 agaaacttca tttatctatg agttaagcaa gtcaaatttg aaatatactt ataatgaaga
      301 tgaacttgaa attttagata atccagaaaa ttcacaatgg attaaagagt tgaagagaga
      361 agactttatt gagacactaa gaattctaaa atcgttattt gatttgaaat tagccaaagc
      421 taccatcatg ggaattgaaa gagattcata ttattgggag gaaattggac tatatgctcc
      481 ccagattgaa ggtgaagata aagagaaaag gatagacaa
//
LOCUS       DACCXW010000324          482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.337, whole genome shotgun sequence.
ACCESSION   DACCXW010000324 DACCXW010000000
VERSION     DACCXW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.337"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>482
                     /locus_tag="IVY71_003063"
     CDS             <1..>482
                     /locus_tag="IVY71_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7987023.1"
                     /translation="VELARDSANKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPY
                     GYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQ
                     FGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNV
                     G"
BASE COUNT          168 a           93 c           97 g          124 t
ORIGIN      
        1 gttgaattag cgagagattc cgctaataaa tacggtattc cacttacatt agacacaccg
       61 tataatcaac caggaatcaa atcacattta tgggtaactc aaaacatttg gggcgatcac
      121 actgatccgt atggatactt atcagaaatg ggtgtaagta aggaaaaact ggcttacgat
      181 ttagctcatg gatttacaga cgaaaaccca acgacttctg agaacaagcc tgtcattgat
      241 ccaacccgag caggtgcagc aaatccaact ttatcagatg gaacgaacca ttctcacatt
      301 gatcagtttg gagaaatcga aaatgcgaac ttacacgtcg ctggatggca catcgctaac
      361 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
      421 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
      481 gg
//
LOCUS       DACCXW010000325          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.338, whole genome shotgun sequence.
ACCESSION   DACCXW010000325 DACCXW010000000
VERSION     DACCXW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.338"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            7..>479
                     /locus_tag="IVY71_003064"
     CDS             7..>479
                     /locus_tag="IVY71_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP7987024.1"
                     /translation="MQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGF
                     NSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEK
                     GVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          174 a           87 c           80 g          138 t
ORIGIN      
        1 gaaattgtgc aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta
       61 gaaaaagcga taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa
      121 tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc
      181 aaaaccgaat atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa
      241 caacaaactt taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat
      301 ttattcgaaa aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt
      361 catcactata ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat
      421 gcctttaaat ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DACCXW010000326          472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.339, whole genome shotgun sequence.
ACCESSION   DACCXW010000326 DACCXW010000000
VERSION     DACCXW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.339"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            194..412
                     /locus_tag="IVY71_003065"
                     /pseudo
     CDS             194..412
                     /locus_tag="IVY71_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          162 a           66 c           76 g          168 t
ORIGIN      
        1 atttttctaa caaaagcaca tattttaata tatactacct attttaggta gtatatattt
       61 ttagatacaa tatcgatcag ttttgaggaa atgaaattct agatattttt tgctacatag
      121 agttactttc aatatttttt aacgtaatta ggcagaagtc actcatcctc tataggtttg
      181 tgatgaaatt gccattgatc catttaagta agagcaaaaa actggaggtt tatcaggtaa
      241 ttggtctaat ggatttaagt atgcggtcac cgtgtataga gttgcttatg agattcaaga
      301 aaataaagtt attccagtat tgctatatgg aacacataac aatttttatg aattagccga
      361 gaacactcgc aactttattc gtgtagatga atcggcttcg gttgagttat aactttagct
      421 atatcaatca accgaccgac atatgttaaa tctatatata ataaaaatac ct
//
LOCUS       DACCXW010000327          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.340, whole genome shotgun sequence.
ACCESSION   DACCXW010000327 DACCXW010000000
VERSION     DACCXW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.340"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>468
                     /locus_tag="IVY71_003066"
     CDS             <1..>468
                     /locus_tag="IVY71_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7987025.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DACCXW010000328          458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.341, whole genome shotgun sequence.
ACCESSION   DACCXW010000328 DACCXW010000000
VERSION     DACCXW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.341"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          155 a           90 c           74 g          139 t
ORIGIN      
        1 taccccggcc taatctggca aggggaggag gagatataat gaaatacctt tttgattttg
       61 tttttgcaat caagccactt gcagatgtag tgtgcagtta cctctgcaaa tggcttgatt
      121 gcaaaattgc caaaaaagac agtaagccta cccgttaatt ctataatata gaaacgtaaa
      181 aaaaacccca tcagcctgca cgctgatggg gtttggtgat ataatgaaat acctctttgt
      241 tactcataat tataccataa ttagtcacga atgcaaccaa tagaaaaaac tacttagaac
      301 ggtagatcat cgctaataac aatgcattgt ccaccaaatg gattttgtga agcattaaat
      361 acattgttat taattgtcgc tgcttttaac taggcctctc cccttctatt ataaaagaag
      421 atatatatca tttcttaatt acctactacc ttaaacca
//
LOCUS       DACCXW010000329          450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.342, whole genome shotgun sequence.
ACCESSION   DACCXW010000329 DACCXW010000000
VERSION     DACCXW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.342"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(35..271)
                     /locus_tag="IVY71_003067"
                     /pseudo
     CDS             complement(35..271)
                     /locus_tag="IVY71_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           76 c           77 g          151 t
ORIGIN      
        1 tagaaggact gaccttctgt ctcagatttt tttgttattt cacttgaatc aattgattga
       61 tcaaaaaaat cctgattttc atgttctcaa atgatttaaa tccataagat aatcgtttca
      121 gtgtcttgat atggggaatc ttggcctcta ttttgccatt agaatacggg taaatcatag
      181 cattgtaaat accttcttca tacgttaaaa ggttttgaag tttcgtgcga agttgtgcat
      241 caagtgtttc aggaagattt tttaataacg ccgtttgtat aattaagcga gacaaataaa
      301 aaagccattt atgttacact ccaattgtaa tacccgctaa agcatacaag gagagtgaac
      361 ataaatgact tatacccatc ttacaccaaa cgagcttgta atgatagaag catattttca
      421 tcaagaaact ccggttgcta tcgttgcgaa
//
LOCUS       DACCXW010000330          449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.343, whole genome shotgun sequence.
ACCESSION   DACCXW010000330 DACCXW010000000
VERSION     DACCXW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.343"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..231
                     /locus_tag="IVY71_003068"
     CDS             <1..231
                     /locus_tag="IVY71_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7987026.1"
                     /translation="LIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQA
                     VGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT          167 a           83 c           75 g          124 t
ORIGIN      
        1 ttgattgata gagattactt tactcaggca gacttgatca ttacactttg tggtgatgcc
       61 aaagacaagt gccctgccgt tccaaacaat cacgaacacc ttcactggga cctaaaagat
      121 cctgcacaag ctgtcgggag cgaagaagaa atattaacgg aattcagaaa agtcagagaa
      181 ggaatcaaaa gaaaagtttt agacttaaat caacgtacat cgatcgtata aagaagtatt
      241 caaaatgatt tttcaagcac taagaaacaa tgttaaaaaa atagctttcc atattttaca
      301 aaattgtgcc ttattgccga caaattgtct cttgaacacc gtttaccagt agtcaagaca
      361 tcgtaagcaa ctataaaaaa ttataagaaa ctcaaaataa aaggatgtgc cacaactttg
      421 gccacatcct ctttttttgt atttagttt
//
LOCUS       DACCXW010000331          426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.344, whole genome shotgun sequence.
ACCESSION   DACCXW010000331 DACCXW010000000
VERSION     DACCXW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.344"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            27..>426
                     /locus_tag="IVY71_003069"
     CDS             27..>426
                     /locus_tag="IVY71_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7987027.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNK"
BASE COUNT          154 a           63 c           85 g          124 t
ORIGIN      
        1 tacttattag agtgagggat aaagaaatga gacaactaca caaagaacag attgaaacag
       61 caatggcagc gttaaaggaa aagacaattg atcatgtata ttttacagca tgtgggggtt
      121 cacaagccgt tttaatggct ggtcaatatc tatttgataa agaattaact gtaccttgtc
      181 atgtttatac tgctaacgaa tttaactatg ctataccaaa aggattatct gaaaattctt
      241 tagttatcac ttgttcccat tctggcacaa caccagaaac tgtagaagca acaaaaaaag
      301 caagagaatt tggagcaatg actatttgtt tatctaatga agaaggatct cctctatggg
      361 aagcagctga atatcctgtt cactatgatt ggggtaaaga tgttgatgct agtgatttaa
      421 ataaag
//
LOCUS       DACCXW010000332          421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.345, whole genome shotgun sequence.
ACCESSION   DACCXW010000332 DACCXW010000000
VERSION     DACCXW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.345"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..390
                     /locus_tag="IVY71_003070"
     CDS             <1..390
                     /locus_tag="IVY71_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7987028.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          163 a           74 c           65 g          119 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 a
//
LOCUS       DACCXW010000333          414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.346, whole genome shotgun sequence.
ACCESSION   DACCXW010000333 DACCXW010000000
VERSION     DACCXW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.346"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(109..>414)
                     /locus_tag="IVY71_003071"
     CDS             complement(109..>414)
                     /locus_tag="IVY71_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7987029.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DACCXW010000334          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.denovo.347, whole genome shotgun sequence.
ACCESSION   DACCXW010000334 DACCXW010000000
VERSION     DACCXW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.denovo.347"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..318
                     /locus_tag="IVY71_003072"
     CDS             <1..318
                     /locus_tag="IVY71_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7987030.1"
                     /translation="ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYS
                     TNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLE
                     SMF"
BASE COUNT          161 a           66 c           54 g          128 t
ORIGIN      
        1 gcaaataatc aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt
       61 aaattgctca tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga
      121 actatttatt caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa
      181 agaaaagagc aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac
      241 aactataatc aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca
      301 ttagaatcaa tgttttaact tgatcaacag aatgggcttt tccatttaca cataattatt
      361 gacacaacct attctggaaa cttacaaata aaagcttatt cttcagcca
//
LOCUS       DACCXW010000335        14660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.068, whole genome shotgun sequence.
ACCESSION   DACCXW010000335 DACCXW010000000
VERSION     DACCXW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.068"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            125..1171
                     /locus_tag="IVY71_003073"
     CDS             125..1171
                     /locus_tag="IVY71_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987031.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            1173..2432
                     /locus_tag="IVY71_003074"
     CDS             1173..2432
                     /locus_tag="IVY71_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7987032.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            2438..3019
                     /locus_tag="IVY71_003075"
     CDS             2438..3019
                     /locus_tag="IVY71_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7987033.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3101..3976)
                     /locus_tag="IVY71_003076"
     CDS             complement(3101..3976)
                     /locus_tag="IVY71_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7987034.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            4124..4456
                     /locus_tag="IVY71_003077"
     CDS             4124..4456
                     /locus_tag="IVY71_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7987035.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            4568..5776
                     /locus_tag="IVY71_003078"
     CDS             4568..5776
                     /locus_tag="IVY71_003078"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7987036.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            5930..6385
                     /locus_tag="IVY71_003079"
     CDS             5930..6385
                     /locus_tag="IVY71_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987037.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            6747..7022
                     /locus_tag="IVY71_003080"
     CDS             6747..7022
                     /locus_tag="IVY71_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987038.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            7035..9113
                     /locus_tag="IVY71_003081"
     CDS             7035..9113
                     /locus_tag="IVY71_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7987039.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            9273..11159
                     /locus_tag="IVY71_003082"
     CDS             9273..11159
                     /locus_tag="IVY71_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7987040.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            11171..12118
                     /locus_tag="IVY71_003083"
     CDS             11171..12118
                     /locus_tag="IVY71_003083"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7987041.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            12138..12665
                     /locus_tag="IVY71_003084"
     CDS             12138..12665
                     /locus_tag="IVY71_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7987042.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(12728..13381)
                     /locus_tag="IVY71_003085"
     CDS             complement(12728..13381)
                     /locus_tag="IVY71_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7987043.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            13513..14355
                     /locus_tag="IVY71_003086"
     CDS             13513..14355
                     /locus_tag="IVY71_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7987044.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            14513..>14660
                     /locus_tag="IVY71_003087"
     CDS             14513..>14660
                     /locus_tag="IVY71_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP7987045.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKK"
BASE COUNT         5026 a         2478 c         3060 g         4096 t
ORIGIN      
        1 tagaaaacac atgtatttta tttaattttt atttatttgc aagttatgag gtgagctttg
       61 ctcttatctt ttctagaatt tgcttactaa tggtaaaata aaaataagaa gaggagggat
      121 gcgtatggaa cttgattatc aagtagaaga tgcgttgacg aaagtgtttt acggagaaac
      181 gctagtcagt cagctaagag aactttccat tgatcagcca atcttatttt taacgaatca
      241 gcgctattat gatttgtttg cagataagat caacccgtta tttgcaaatc aagcaaacaa
      301 tgactggtat atttgtgcca acactcaatg caatcattta acagaattaa agaacctgct
      361 tgatttcgtc aaacgctatc ccgaaaatca atccatgttt atcatcggat ttggaaatga
      421 agggatcatg gatctggcag gtttttttca aaaacataca acccttgatt catcactatg
      481 gatgatccct gtatcgatcc gttctctaag ccgagcatta acaccgcagc ggacgatttt
      541 gcagctacca aatcgtgtag tgctgcaaac catcaatctg ccagaacgca tcgtttatga
      601 tcaaacaatc agcgagcggc aggtggacgg aaagcagatt gactttttga cttttatctg
      661 ttgcggaatg atttgtgatc atcgtttttt gcaaaatctg tataaaaact atccaaataa
      721 aaagcaatta acgactcggc cgtttgccgg gatgatcgaa gaaatgatct atttttatca
      781 attagaggca gaaaacctgt caaattatgg taaactgttt gaacaagctt tttatctgac
      841 cgaaaatggc catctacttt ctgctagtat gaaaaaattt ttaggaatga tgtttcattt
      901 cgtgtggaat ctccaagcat atccttttga tttccaacta aaaaactttt ttgtgtggct
      961 ggacttgctt ggctatccaa tccactggct ggaacagatt tcaaagatgg agtatttgga
     1021 aaaagttctt tttttggcag aaaaaagcaa aaaaatactt gttttagaaa aaataggtat
     1081 aatagacgga taccaacatc caaacgaaaa agaattattg agaacaatga cggtatttca
     1141 acaaatcgtc agtgagataa gagggaattg aaatgaatac aaatagcgaa aaaatgttgc
     1201 aagctttatc agatgaagac ttgtcacaag cacaaatctt tttagaaaaa gcattaaaag
     1261 aagacccagc agatatatta gctgaactag gcgaagaatt gctggctatt ggctttttga
     1321 tcgaagcaaa acagattttt gaacaactga tcaaccaata cccagaaaat gacggactga
     1381 acatcccgtt agcagaaatt gcaatcgaag ataatgagat cgacttagct tttgaatacc
     1441 tggaaaaagt ggataagcaa agtgattact atccgcaaag cttattggcg atagctgatt
     1501 tgtaccaagt tatcggaatc cctgaagtga gtgaagcaaa attgaaagaa gcagctgggt
     1561 tgcttcctga tgaaccattg atccagtttg cgctagctga attgtatttt tcagttgatc
     1621 gtttcaaaga ggcagcagtg gtttatgaaa cattgttggc aagcgggata aatgaaattt
     1681 caggaatctc gatgcaggaa cgtttaggac aatccttaag tatgcaagga gaattcgaag
     1741 cagctatcgt acctttagaa gaagcactga aagaagaaag gacagatgat cgactgttcc
     1801 agctagcttt tacgaatctt caattgaaag aaaatcagca ggcaatcaac tatctggaag
     1861 aacttcgaga agttaatccg cattatcaat cgttatatct atatctagga caagctcttc
     1921 aagaagaaga aatgattgaa gaagcacaaa cagtgttaga agaaggaatc aaagaaaatc
     1981 cttaccaagt cgaactttat cacctggcct cggaaaatgc tttccgtctg catgatagac
     2041 agcgagcaga agaactttta ctgaaagctt tagaagtagg agacaaacaa gatgaaacat
     2101 tacttacttt aagtaatctt tacttggatg atgaaagata tgaagatgtg atacaagtga
     2161 tcaatcagat ggaagaaact gctaatcctt atgcagaatg gaacctagcc catgcttata
     2221 atgaattaga agactttacc gttgcagctg tacactatga acaggcttat catgaactat
     2281 cacatgaacc agatttcttg aaagagtatg cctttttctt aagagaagaa gggcagctga
     2341 aaagagcgca agaattattg acttattatt taacacttga accaggagat atggaggcac
     2401 tgtcattatt agatgaccta acagagaggt gagaaaaatg ctaattgatg tacgggaaaa
     2461 gaagaatttt ttaacgtgga tggtcaatca tgtatctttt agccgaagag aagttttttg
     2521 gatcttgaat tatcttgcca atcatgaagc gattttaagc aatgtccact ttgttgaagg
     2581 ggcgaatttt gcggatcgag gactgcagat cagtgatcta tctgttgaag gggaacccat
     2641 gaaattgttt ttgcagggaa aagaatttac ggacacagac cagatttttc atgaaatccg
     2701 tttgaattgg aaaaagcctt tatatgtgga atgtatattc gaaaatgctt ggcagacaag
     2761 agaatatcta tctatcttag aagacaatcc attttcttct tgggattctt cagtcagtga
     2821 agaagatact gaattaattg aacgatattt tcaagaaaaa gaagaagagg caaaactaaa
     2881 aatgctttat gctcaaatcg atcaagcact agaagatgga gatagggatg cttttttgaa
     2941 attatctgat gaagttaacc gaaagatcct tttgcagaca caaaagaaaa gtaaccgggt
     3001 aagtgaacat aacaattagt taaaaatagt atagtactaa aaaagacaaa ggtcagctgg
     3061 atgtggaatc tttcctcata gcctatcttt gtcttttttc ttatagtttt ttcagtaatc
     3121 ttgtctgtgg cttcaaataa gtaaagcctt gcccttccac ttcatgcaca ttgatgattg
     3181 aaaggaaagc tttttcgtca atctcgtgga cgatcctctt agcttcagtt aattcactag
     3241 agctaacgac catatagatg attttttttg aattaccaga gaatgcgcct tctccagaaa
     3301 ggaaagtcac acctctttgc agaccggtca tcagtagtgg tgcaatctct tcgcttttgt
     3361 tggagacgac aagtagacct tttgcagaat atccgccatc taagatcgaa tcaatcacac
     3421 gactgaaaac atacgaaaca atcaacgtat acatcatacg tttcaagtcg atataggtta
     3481 atgaagcaag taagacgatg atatcaaatg ccaataggga acgccccata gtgatgccgt
     3541 aattttgttc taaaatccga gcgataatat cactgccgcc tgttgttcca cccatgcggt
     3601 agacgatccc gcttcctaca cctgcaacta aacccgcaag tagagaaacg atcagtagat
     3661 catgctgtaa attgatttct agtggaaccc tttgccagat ccataaaaag aatgacaaag
     3721 aaagtgtacc tagaacagta taggccaagg aacgtttacc gaggatcttt cctcctagta
     3781 aaatcaatgg gatgttgatt aataaagtcg tataagctgg atcaatacca aataaagctc
     3841 ttaagatcaa ggtgatccct gagactcccc cttctgctaa atgattagcg atgttcaagt
     3901 aaacaagacc aaatgcataa atacttgttc ctataatgat aaaaagaact tcttttactg
     3961 tttttcgttt gaccatatga attccagctt tcaattatgt attttcacag aatttccatt
     4021 tagaaattcc ttcctaaatg tagcatggaa attcgatttt ctcaattgct cttatctagg
     4081 atttgcgtta aagttttggt aagatagttt tggagggaaa aagatggata aaaagcgttc
     4141 tttatacagt atgcagcagg aagtggatga atatatccag caattcaagg tggggtattt
     4201 ctctccatta gctcagatgg ctcgattaac tgaagaagta ggtgaattgg caagagaagt
     4261 caaccacact tatggagaaa aaagcaaaaa ggcttctgaa ccagttaact cggtagcaga
     4321 agaacttggt gatgttttat tcgttacgat gatcatggct aattcattga atatcgactt
     4381 aactgaagtt tttgaaaaaa acatggaaaa attcaatcaa agagaccata atcgctttga
     4441 gcgtaaggac gagtaaacaa gatgaagtgg tcctggcctt gaagactctg aaacggactt
     4501 gtcaaacagt caccatgcaa ctgagtcttt atctcaggac aagaaacacg caggaaaggt
     4561 tggaaaaatg aaattaaaac aattacctct ggaataccaa aaggcaattc cagttctgaa
     4621 aaaattagag aatgcaggat atgaagctta ttttgtaggt ggaagtgttc gtgatatctt
     4681 gctgaaccag ccaatccatg atgtggatat cgcaacaagc gcatttccag aagaaatcaa
     4741 acagcttttt ccaaaaacga tagatattgg gatcgaacat ggaactgtgt tggtattaga
     4801 aaatgatgaa caatatgaga tcaccacatt tcggacggaa tcaacttatc aggactaccg
     4861 tagaccagat catgtcgaat tcgttcgttc gttggcagaa gatttgaaaa gaagagattt
     4921 tacgatcaat gcgtttgctt tgaaagaaga tggagaaatc atcgatttgt tcgatggtct
     4981 gcaagatcta gaagaacaga tcttgcgagc tgtaggcaat cctcatgagc ggttccatga
     5041 agatgcctta cgaatgatgc gcgggttgcg ttttgtcagt caactagggt tcaaattaga
     5101 ggaagaaacc ttctacgcca tcaaagaaaa ccacgagcta ttaggtaaaa tatctgtaga
     5161 acgtataaat gtggaattca tcaagatgct cttgggaaat gaacgggccg cgggaatcca
     5221 tgcatttatt gaaacagagt gctacatttg ctgtcccggt ttgaaagaag aaggcgaagc
     5281 acttttacgt tttgctgaat taccatccgt cccattgaaa gaagagagcc aagcatgggc
     5341 attattgatc agccaactgg atctaaaaga aaatgagatt cgttcctttt tgaaatcgtg
     5401 gaagtgttct aaccaaatga ttaaagacgt tcaagcactt gtccaaggac tacgacaacg
     5461 agaaactgga gctttggaac cattcgaatt gtacttctta ggcaaaaaac atgcactgca
     5521 agtagaagaa ttgatgatct attttgacga aacacctcag atggatgccg taggagaatc
     5581 ctatgatcgt ttacctatca agtcattaag cgaattagca gtagatggta aaatcttgct
     5641 agaagaaact ggaaaaaaac ctggtaagtg ggttagtgaa gctttgcaat tagctgaaaa
     5701 agccgtcgta gaacaaagaa taaaaaatga taaaaaacag gttttggatt ttttatcgaa
     5761 agaaaaattg atttaaaaaa cactagactg tatctgctca gctttgttgg ctacttaaag
     5821 tgggcagata cgtcattaga agaaaaaaca taagatgtga ccacttacat aaatttgtgc
     5881 taaactgagg tagtacaaga ataaattaca tcataaagga gtctttttaa tgagtaaaga
     5941 aatgacagcg ctaaaatttt attttagaaa tggagaaacc tggacgatca accgccgtca
     6001 tatcggcgat ctttggatca aacaaatcac aacaagtttt ggacgaatca atggcagtga
     6061 atttgttgaa atccatccgt gtgctggatt taaaatcgaa atcttccatg aaggggacgc
     6121 agttgcgaca catgatatca acctaggcgg actggaaatg ggcatgttta atcgcgcatt
     6181 gaaatacgaa gatatcgagc gaatggaaat cctttaccga aatggcacac ctgatcttgt
     6241 gtacttccca tatctagata aaggaacaga aggattagac aatcaatatc aatcaacaaa
     6301 aatcagcgaa aaaacaggta acttgtatat cgtgatcaat ccagatcaac gtgtagaaga
     6361 tgtatacggg gaatttttcg aataaatcga tttttacgca aaaatgaggc tgtgaaagag
     6421 atgtgtctta ttactactga taggctgtct cttccacacc gtttattcag aataagggac
     6481 tgtgacaaga tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca
     6541 ggaatgggaa agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga
     6601 tatctgatac tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag
     6661 caataaacga tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg
     6721 aagtttgtgt ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt
     6781 tgcatgatcg ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc
     6841 gtaaaagaaa tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt
     6901 tggagtcaaa acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg
     6961 aaaccaaaat tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat
     7021 aaaggggatg gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct
     7081 tatcgaacat agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc
     7141 ttggagtggt attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt
     7201 tcttagttat gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt
     7261 cgattctcaa aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc
     7321 ttgtcggggt aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct
     7381 ttgcttttgg agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac
     7441 gagtgaatat ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg
     7501 catctggtgt tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag
     7561 cagtaaacgc ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt
     7621 tgcttgtctg gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg
     7681 tcacagggta tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg
     7741 catttgatgt atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt
     7801 ttaggaaaat ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa
     7861 ctattgcttt tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag
     7921 tgttctcgcc agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg
     7981 tattaatctc tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat
     8041 tcaaagatgg cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg
     8101 gcggtgtaaa gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata
     8161 atatgatgtt ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga
     8221 ctttggtcat agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca
     8281 cggatttgaa tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa
     8341 aggaaattga tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc
     8401 aagaacggat acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac
     8461 aagaaataca agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt
     8521 cgggcaagct ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac
     8581 attctatcac aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt
     8641 taatgcgaat cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga
     8701 catttattag tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt
     8761 tgattattga aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt
     8821 ttatccagca acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt
     8881 ggatgatcca gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa
     8941 gaaaaatcat tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct
     9001 atcgcaggaa tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt
     9061 ctttccgatt tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta
     9121 aaaattttca agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt
     9181 ctttttttga gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt
     9241 ttatgataca atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc
     9301 tcaaaaaaac ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa
     9361 aagaccgtat cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta
     9421 ttgccggtat cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc
     9481 gtattggtta tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag
     9541 tctttcaagg aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg
     9601 aactagggga aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac
     9661 aaatgaataa agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat
     9721 taggaatcag tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag
     9781 ttagtctggc gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa
     9841 accatttaga ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg
     9901 cactattgat ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg
     9961 aactttcatt tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa
    10021 aagcagaacg tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac
    10081 aggaattagc ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga
    10141 tcaaccgttt cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag
    10201 agctgaacct agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt
    10261 accgtataaa tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa
    10321 gactaggtat tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg
    10381 gcagaattcc cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt
    10441 atacacaaga aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag
    10501 ctgctgaaga agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac
    10561 gatttttatt tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa
    10621 aacgccggtt atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg
    10681 aaccgaccaa tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt
    10741 ttccaggtgc tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata
    10801 agttattagt attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt
    10861 atttactgaa gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga
    10921 gcaaggaacc tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga
    10981 ttgaagatga gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc
    11041 atcaaggaga taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag
    11101 cacaacttga agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg
    11161 aggaaaaagg atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca
    11221 tcgtaaagaa gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt
    11281 tgatctgcaa aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa
    11341 aagcgaattg ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa
    11401 aaaccatatt tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg
    11461 accagatatg actgattttg gccatagatg tttgacagat cctgaattca atgaagaata
    11521 taaaaagcag agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac
    11581 atacggcgat cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg
    11641 tggctttatc gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag
    11701 aagattgatc gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg
    11761 tcatacgatg tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg
    11821 cagtggggat gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca
    11881 cctaattgca catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc
    11941 tcatctttat atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc
    12001 atttccgact ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga
    12061 tatccaatta gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa
    12121 tcagaaagga tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg
    12181 gaaaagaaga ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga
    12241 cagaagcaaa tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt
    12301 tgccgaatcg tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc
    12361 atgtcatgca tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt
    12421 tcatcgctgg agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc
    12481 gtacagtcat ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg
    12541 attggtcact gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc
    12601 aatttgaaac gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga
    12661 tataaaaaag ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt
    12721 ttttttatta agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa
    12781 acaaatgcca aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta
    12841 tcgtgtaaga aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat
    12901 aaagaggaac agcagccacg acacagagcc accccataaa aatatagatg atcgtagata
    12961 cttttgatac tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg
    13021 caaacaacca gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac
    13081 aaaaaggagt ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga
    13141 acaccttttt tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa
    13201 gaatcatcat cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg
    13261 caccttttac taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg
    13321 tgactgcatt aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca
    13381 tgaaaacggc tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta
    13441 aaaatagaaa ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag
    13501 agagagaaaa taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa
    13561 aatgtacgcg atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca
    13621 gtatatcccg atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca
    13681 aaaaatctgc ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag
    13741 ctaggagaag acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca
    13801 gtagaagcag caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt
    13861 gataccactg accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa
    13921 ggtaaaacag tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta
    13981 tacatcggaa tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact
    14041 ggtttgctgt ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta
    14101 atccctgtaa ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa
    14161 aatgaactga aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa
    14221 gaattggcgc aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct
    14281 gtactgcata cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg
    14341 tattatgtag attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt
    14401 ctctttttct ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt
    14461 ttgataaaat ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt
    14521 aacgaaacga catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc
    14581 aggtttagct gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag
    14641 agaagatcca aataaaaaag
//
LOCUS       DACCXW010000336        10276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.101, whole genome shotgun sequence.
ACCESSION   DACCXW010000336 DACCXW010000000
VERSION     DACCXW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.101"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..2450
                     /locus_tag="IVY71_003088"
     CDS             <1..2450
                     /locus_tag="IVY71_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7987046.1"
                     /translation="DYSFGYVADWSSGRELSELKASLETIRATASELITEIDGHFAQL
                     QQEREARQEQEAVTVDVTPEAAAPAQAEEEQAPALDPAAEPLVTVLWSESPQLKDGQQ
                     MPLHEADALFARLDAAYPAEAGYDKTAFRIDCIFQGEPDSYEGRQDFGDRDGSLIEHI
                     QSYHQFYAQDENWKKHVIATTGIEGWEQDKAHRDMLLGEFVPYMKLHCSLAQMEQTAQ
                     GRLQSGETLTPEETAYLGALLSYVQECRPMLNQGQYQLPEPPKLADFDQSLQDYKAQV
                     QAEIAQEAAAAGMTVEEYAAAGYEAPQQDTFSIYQLRGGEETRDYRFEPYERLQAAGL
                     AVDSANYDLVYTAPLDKSTSLEDIYRTFNIDHPADFRGHSLSVSDVVVLHQNGQDTAH
                     YVDSFGYMEVPEFLRENPLRTAELSTEQNENMIDGIINNTPPEPQPEEPEQLSFEDVA
                     VASGQTQEEPPEAAPPQTAVRYYPINEGAARRAKEAMSFSDYRPGSATAEYRHYVDQA
                     VEIAERQKKRVEPEYHEKIDSLLDTYARKLAENMNKGYEITARVPSVLIAGPSNFPVR
                     AKEKQNAASDRNMEEFQYIQGLLDKIRSTGMGGISADDPQAVSKLEKKLEKLEASQEL
                     MKAVNAYYRKHGTLDGCPHLIERGIENLKADMASGWHYEKKPFQSWQLSNNNAEIRRL
                     KGRIEELTRQKEAAYVGWEFDGGTVEINRVANRLQIFFEGKPDAAVRDELKSNGFRWS
                     PKAGAWQRQLNDTTIRVADRIKCIQPLSGEKPSALQLAARREKEKPSIRAQLNAAKTE
                     QAKQPPAREKSKGLEVE"
     gene            2451..3395
                     /locus_tag="IVY71_003089"
     CDS             2451..3395
                     /locus_tag="IVY71_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987047.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            3399..3602
                     /locus_tag="IVY71_003090"
     CDS             3399..3602
                     /locus_tag="IVY71_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987048.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(3902..4138)
                     /locus_tag="IVY71_003091"
     CDS             complement(3902..4138)
                     /locus_tag="IVY71_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7987049.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(4321..4692)
                     /locus_tag="IVY71_003092"
     CDS             complement(4321..4692)
                     /locus_tag="IVY71_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987050.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(4689..6017)
                     /locus_tag="IVY71_003093"
     CDS             complement(4689..6017)
                     /locus_tag="IVY71_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7987051.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(5978..6307)
                     /locus_tag="IVY71_003094"
     CDS             complement(5978..6307)
                     /locus_tag="IVY71_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7987052.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(6565..6935)
                     /locus_tag="IVY71_003095"
                     /pseudo
     CDS             complement(6565..6935)
                     /locus_tag="IVY71_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7080..7265
                     /locus_tag="IVY71_003096"
     CDS             7080..7265
                     /locus_tag="IVY71_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7987053.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            7777..7995
                     /locus_tag="IVY71_003097"
     CDS             7777..7995
                     /locus_tag="IVY71_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7987054.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            8208..8867
                     /locus_tag="IVY71_003098"
     CDS             8208..8867
                     /locus_tag="IVY71_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7987055.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            8870..10213
                     /gene="vanS"
                     /locus_tag="IVY71_003099"
     CDS             8870..10213
                     /gene="vanS"
                     /locus_tag="IVY71_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7987056.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         2539 a         2592 c         2736 g         2409 t
ORIGIN      
        1 cggactattc ttttggctat gtggccgatt ggagcagcgg cagggagtta tccgagctga
       61 aagcctccct tgaaaccatc cgggccacgg ccagcgaatt gataaccgag atcgacggcc
      121 attttgccca gcttcagcag gagcgcgagg cccggcagga acaggaggcc gtcacggttg
      181 acgttacccc ggaggcagcg gccccggcgc aggccgagga agaacaggcc ccggcccttg
      241 atccggcagc cgagccgctt gtgactgtgc tatggagcga aagcccccag ctaaaggacg
      301 gccagcaaat gccgttgcat gaggccgacg cgctgtttgc ccgtctggat gcagcatatc
      361 cggcagaggc aggctatgac aaaaccgcgt tccgcattga ctgtatcttt cagggcgagc
      421 cggacagcta cgaggggcgg caggattttg gagataggga cggttccctg atcgaacata
      481 tccagagcta ccaccaattt tacgcgcagg acgaaaactg gaaaaagcat gtaatcgcta
      541 caaccggcat agagggctgg gagcaggaca aggcccatcg ggatatgctg ttaggcgagt
      601 ttgtccccta tatgaagctg cattgcagcc ttgcccaaat ggaacagacg gcgcagggcc
      661 gcttacagtc cggggaaacc ctgacgccgg aggaaacggc ctatttgggg gcgcttctct
      721 cttatgtgca ggagtgccgc cccatgctca atcagggcca gtatcagttg ccggagccgc
      781 cgaaactggc tgacttcgac cagagcttgc aggactacaa agcacaggta caggccgaga
      841 tcgcgcagga ggccgccgca gccggtatga ccgtggagga atacgcggcg gctggctatg
      901 aagcacccca acaggacacc ttttccatct accagcttcg aggcggggag gaaacccgcg
      961 actatcggtt tgagccatac gagcgtttac aggccgcagg gctggccgtg gacagtgcca
     1021 actatgacct tgtttatacg gcaccgctgg ataaaagcac gtctttggag gatatttacc
     1081 gcacattcaa catcgaccac ccggcagatt ttaggggaca cagcctttcc gtttccgacg
     1141 tggtggtgct gcaccagaat ggacaggaca ccgcccacta tgtagacagc ttcggctata
     1201 tggaagtgcc ggagtttttg cgggaaaatc ccctgcgtac cgccgaactg tctacggagc
     1261 agaacgaaaa catgattgac ggcattatca acaatacgcc gcccgaaccg cagccggagg
     1321 aacccgaaca gctatctttt gaggatgtgg cagttgcttc cggccagaca caggaggaac
     1381 ccccggaggc tgcgcccccg caaacggctg tccgctatta tcccatcaat gagggagccg
     1441 cccgccgcgc caaggaagcc atgagctttt ctgactaccg gcccggcagc gccacggcgg
     1501 aataccggca ctatgtcgat caggccgtgg aaattgcgga gcgccagaaa aagcgtgtgg
     1561 agccagagta tcacgaaaag atagacagcc tccttgacac ctacgcccgc aagctggccg
     1621 agaacatgaa taagggctat gagattaccg cccgcgtccc ctctgtcctg attgccgggc
     1681 cgtccaattt cccggtacgg gcgaaagaaa agcagaacgc cgccagtgac cgcaacatgg
     1741 aggaatttca gtacattcag ggcttgcttg acaaaatccg cagcaccggc atgggtggta
     1801 tcagcgccga cgatccgcag gccgtttcca agctggaaaa gaaactggaa aagctggaag
     1861 cgtcgcagga gttgatgaag gccgtcaatg cctactaccg caagcatggg acgctggacg
     1921 gatgccccca tctgatcgaa agaggcattg aaaacctgaa agcggatatg gcgtcgggct
     1981 ggcactatga gaaaaaaccg ttccagtcct ggcagctctc caacaataac gccgagatac
     2041 gccggttaaa gggccgcatt gaggaattga cccgccaaaa ggaggcggcc tatgtgggat
     2101 gggagtttga cggcggcact gtggagatca accgggtggc caaccgcctg caaatcttct
     2161 ttgaggggaa gccggatgct gctgtacgcg acgagctgaa aagcaacggt ttccgctggt
     2221 cgcccaaggc cggggcatgg cagcggcagt taaacgacac cacaatccgc gtcgctgacc
     2281 gtatcaagtg catccagccc ctttccggcg aaaagcccag cgccttacag cttgcggccc
     2341 gcagggagaa agaaaagccc tctatccggg cgcagctcaa cgcggccaag accgaacagg
     2401 ccaagcagcc accggcgcgg gaaaaatcaa agggattgga ggtagagtaa atgcagaagt
     2461 ttgaaaatgt ggacgttctg gccgcgctgg aacagatcat gcggcagaac accgcgtttt
     2521 atcaaagtga ctttggcatt gacaaagaca ttatcaggcg ggatgccgcc agcgatcagg
     2581 ccgcagacaa gaccctgctc tggatgtccc ggccctccgg gacgtactgt ttccgggaac
     2641 gtgatgtttt cctgaaagac acccgacagt acaacacatg gaaattttac ggggagcaga
     2701 cccgtgacaa gatactggcc tacgcggtgg agctgaccgg cacacagggc ggcactatcc
     2761 ggggcaatct ctatgagctg gactatccgc agcatttccg gcacgtcatt gaaaccgccc
     2821 agccggtgag tgtgaaccgg ctcttttatg agcatggcac ccgtgacatc ccggagagcc
     2881 agcgttttga cggaagccct gaccgggtat tgggaaactt tttgtactat gaggcccagc
     2941 cccatgaccc ggctgtgctg caggaggcgc tacggacgga gcagcacagc cgggggcggg
     3001 ccgtccccgg cgatttcaaa gtccacgtca ccgctttgca tgacagccgg attgaaaccg
     3061 aggcccggcg tattgtggac agcctgaaag atctgtccgc gcctaacagc cccaataaaa
     3121 cgcatttcat ggtggagatc tccccgcact ttgcggcgat tgcttccacc aaggataatg
     3181 accggctctt tgccatgctg ccctataaat ccctgtgttt taccgggatg aaaggcaggc
     3241 atggcgtgta tgccgtgatt aaccgggacg agcgccggga tgtgagtatt cgcaggcccc
     3301 ggccctctat ccgcaagcag ctatccgaca gcaaacaggc cgccagccca aaaaaggcgg
     3361 cggccagaac caaaaagaat gaattggagg tataaggaat gatgcacttt accgtggagg
     3421 aagaaaacct gatctgtatg taccacaatg cagaccggcg cagaacgata aacaggctca
     3481 ccgccgtcat gccggttatg gatggggata tgcgggcgca ggcccagcag accctttcca
     3541 agctggaacg catgacggat gccgactatg acggccagaa gttccacttc acagacgaat
     3601 aacgaacagc agacgccggg cgcggcggcc atgtggtcgc ttgcgtccgg cgtcttttct
     3661 ttttgcctat tcctacgcat ttagaacccg gtattgtcgg ggataggatg aagtattcgc
     3721 ttgcccctgc aaacatgcgg gagaagcctt gcagcgggcg gttttcaatc ccttaaatgc
     3781 gtaggttgca gctataatta gacccctgaa acagatatag ccgcgcagcg ggtgtatctg
     3841 tttcaggggt gtgcaggtga tagccgcaaa gcggcgtaag agggtgcagc cctcttgccg
     3901 tctatctctg ttccagcggg aagctcggca atgcttccag cagtttcagg gaaatgcgct
     3961 gccgtatatc ttcgtcaatg actttcttct ttttgccgtt tgcctgtctg atctccaccg
     4021 tggaaagctc gttgatgtag tcagcataat gggccaacac tttttccgtg gcccacctct
     4081 caccggcaac ggcggctttg atcgtttcat agtccagcag cttttcttgt ccgtccatcc
     4141 tgataccccc gtcagactga ttttttctat gataccatat tcggaagcat ggaaccagag
     4201 aaaacggcgc ttttctgctg gtaaattcta cgcatttaga acagcacttc ggcggggata
     4261 ggataaaaca cccacatacc ccgctgacca tgccggaaaa gccttgaaat ataagggctt
     4321 ttacagctct aagtgcgtag gtttccagta tcttttccgc tggcgctcgg cctctttgcg
     4381 gcggcgcatc cgtttggagc aggccgcgca gtatttggcc cggttagact tcggcgtgaa
     4441 cgtggccccg cactcacagc agcgtttcac ttcgtcccgg tctttggtga tctcggcata
     4501 cagggccgca tccagcggga ggacagcggc cttaaaccag cggcacagaa gcgaatagga
     4561 aatgctctgt acgcaaatac attcttcgcc gtcgtccagc aacaggcagt tgccgtcaca
     4621 atagttgcag cactcatgcg tcagcttccg ggccttgcga tattgagggt aattcattcg
     4681 tggaatactc atagctcttg cgtccattcc tttcccggcg tcatacgcaa aatgccgtcc
     4741 acattctgct tgatgatgcc gtattcctgc atctgctttt tcgcttcgcc gtacttctca
     4801 tacagccgtt ccttttcctc ggccaatcgc ttgtactcgg ctttcagggc gtacagatcg
     4861 gggagctttt tcacaccctg tccctttagg gcctttgccg cagcttcata caaaatcaac
     4921 gtgctttcat gctcacgccg gaattttgcc ttatccgctg cattgcggta ttccatagcc
     4981 acgggccaga gctgcttgta ggtagaaata ttctttatca gcaccgccat atccgcaagc
     5041 cggtgttcca tagctttgag ggtagcggcg gcctcgtcgc tggcggcggt gatctcggcc
     5101 actttgcttt ccaaatccgg gtaggtgtct atcccatgtt cagtcaggaa attcaaagtc
     5161 tttgcggcct gcttcaaatt gtggagcttc gcccacctct catagcctgc cgactgctgg
     5221 gccttaatgt tgttttccag atcaatccgc aaagaaatcc ccttgcgctc tttgggcgct
     5281 ctggtgataa cgcggccttt gatacgctct ttgatggctt cctcggtgta ggcttccccc
     5341 aatgtcttgc agcgggtaaa gcgttcctgt ccgggagcgc ggaaagaaat aaactttccc
     5401 ggcttgatct catagccctg cgcctgcatg agccgcagga aatcatcaaa atcttttgcc
     5461 tgcgggatag cggcgtcaat ggctgctttg agcttcgctt tccagctcgt ccctttccgc
     5521 tgggcgtccc actcggcata gctcttgccc ttactctggc cgggcttcac cacggacaat
     5581 ccgttttcct tgcacagcct gtcgctggtg cgccggatat aggcatagct ctgtttgttg
     5641 gaaatgtact tgcgctgatt taccatatcc acggcacaga aaataatgtg attgtggacg
     5701 tggcccttat caatatgggt agtcagcaca tagggatatt tcccttgcag cacttcctcg
     5761 gcaagctgct tcccgatctc atgggcctgt tccggcgtgg cttcgccggg ggcaaaggat
     5821 tgtatcaggt ggtggcccag attattgccc ttttggtatg cctgcgccag cagcatttca
     5881 aattcaatgt ccgctgtttc ataagagcag gcaaaggagg ccaccagcag cttgccgtca
     5941 gttttgtccg ggttctcgat gtagtcaagg gcttttttca gcgttgactt gataggatgt
     6001 atctttgtaa ctgccatatc tgcgccagcg ccccctttat atcctcgatg tcctgtgcgt
     6061 acaccggccc cataccgttc agcctctttg caatctgatt gacgttgacg ccgatcttct
     6121 gtatctcggc ggtctgcgcc cggatgtcgc tggtgtccat gttgacgata tagccgtcaa
     6181 tgagcatctt ccgggcgtag gctgaaaagt tcttcgtatg cagcagcgcc attttctgtt
     6241 caatcatagc ccgttcctcc ggcgtcacag ggacgcgcag cacgattttt cgctttctgt
     6301 ttgccatgct tcaccgtcct ttccattcag gggtttgggg atttccccaa caagcaaaac
     6361 ggacgcggcc cggcagggac ggggcaggcc gtttttccgg gagtggctac actccctatg
     6421 cttgctacgt tactgtttct ccactaacaa gacgtttcca aagccccaaa ctacccgcaa
     6481 aatagccctc tggaaagaaa aaagtgcaaa aaaatagaag ctacgctgtg cagctcccgc
     6541 taaaagtaaa acagaggatg ccagttacac atcctccgtt tcattcttct tcggcttttc
     6601 aattagtatg gcgctgtcaa tcgcgccctc gatgatcggc aggtattctt cggggcagag
     6661 tttcagcttg tggctgaccc tttgccgcag ttcgctttcc tcccgcatga tctccgggtt
     6721 aaagtagcgt tccacaggaa gcccgcaggc tttaataagc tgtatgacaa ccggcagact
     6781 gggaatggcc cgtcgttttc gatgttggca agatagcgcc agtcaatatt tattttctct
     6841 gccattgctt tgcgcgaaac gcgctttgct tgccgggccg ctttcacatc cgcgccgaaa
     6901 gtttcaaagc cgggacaatc ttcaatattc gccatatcgc acctcccact tacattgtat
     6961 atttcatact ctgcccgtgg aatgttggta tatgcacctt tcttaaagtt tatatttcac
     7021 attttgtttc ttttattctt cgagcaaaag aattataatg taccctgtgg aggtgtacta
     7081 tggactacat gacgttgaaa gaggcaggcg agaaatgggg tgtgccccca cgatggataa
     7141 actactattg tgctgcggga cgcatccccg gcgctgtaaa gatggcgacg atctggctca
     7201 ttcccaaaac tgcggaaaag ccgattgaca ggcgtacaaa gaaaggaaag gcggttactg
     7261 aatagtataa tgaaaccggc gcattttgga tatggttttg aagagagaga aagcatggta
     7321 ttttgtcacg gaaaaactat aagatgatag atggcaggct gctccagacc gataaaaagt
     7381 attctcatct gaaacagaaa gaaaagattt acacatggat gtatgaagaa gcgaaacttt
     7441 atcatgacag aaatggaaaa tgcccgaaac aaagacgagg aagctgctgc tgtatcagcc
     7501 gtatatggta gaattgaaaa ggccggcatc tggattcttt atggtgaagt ctccagacat
     7561 tggataactg ctggtctaat gcgcctttga gtatggggtg attgatttta ataccgggga
     7621 ggtacggctt tttgaggaaa atattctggg atttacattt ggcgattatt acatttcctg
     7681 taactgcaag gacacggatg atgaagatat gaggaatata gacttgctga ttttctactg
     7741 tcttgaacgg tattaggact gaatctgctg cgcggagtgc ggcaagagcg accggaggga
     7801 gtacacacag gccctgcctg tgtgaaaagc cataccgcaa ggtgcattcg caaaaaagag
     7861 gccgcgcatt ttgctgaggc ttggggtgtc cccaacaagc aaaacgagaa cgccgcaggc
     7921 gggcaagtgg tggatcagaa tatccgtaaa ggatattctg atccattgct tgccagtatt
     7981 atcgggatgt tttgacaacc gctgtaatcg aaacatccca aacattaaga aggtgttaat
     8041 tattattatc ttgatttaat tcgtttaaac caataatata gatacataca ccatgaaaag
     8101 aagcgagtat cccaatgacg gatgattaaa atccgaaaag aaatcgcttt gaaactacag
     8161 ggaaactaca gactgttatg ttatcttctt aaaatggagg gatttttatg tcgatacgaa
     8221 ttctacttgt cgaggatgat gatcatatct gtaatacagt aagggcgttt ctggctgagg
     8281 caggatatca ggtggatgcc tgcacagatg gaaatgaggc atacaccaag ttttacgaaa
     8341 acacttatca actggttatt cttgatatta tgctgcccgg tatgaacggg catgaacttt
     8401 tgcgtgaatt tcgtgcgaaa aatgatactc ccattctgat gatgacagcc ctgtcggatg
     8461 acgaaaacca aatccgggcg tttgatgcag aggcagacga ctatgtaaca aagccattca
     8521 agatgcagat tttactaaag cgggtggaag ccctgctgcg gcgcagcggt gcgctggcaa
     8581 aggaaatccg tgtcggcagg ctgacacttc tgccggagga tttcacggta ctttgtgacg
     8641 gtacagagct gcccctgaca cgaaaagaat ttgaaatcct tttgctgctg gtgcagaaca
     8701 aaggcagaac cttaacgcat gaaatcattt tgtcacgtat atggggatat gactttgagg
     8761 gtgatggcag cacagtccac actcatatca aaaatttgcg ggccaagctg ccggaaaata
     8821 tcatcaaaac cattcgcggt gtaggatacc gattggagga atcataataa tggaaagaaa
     8881 agggattttc attaaggttt tttcctatac gatcattgtc ctgctactgc ttgtcggtgt
     8941 aacagcaaca ctgtttgcac agcaatttgt gtcttatttc agagtgatgg aattacagca
     9001 aacagtaaaa tcctatcagc cattggtgga actgattcag aatagcgata ggcttgatat
     9061 tcaagaggtg gcagggctgt ttcactacaa taaccaatcc tttgagtttt atattgaaga
     9121 taaagaggga agcgtactct atgccacacc aaatgccaat acatcaaata gttttagacc
     9181 caactttctt tatgtggtac atagagatga taatatttcg attgttgctc aaagcaaggc
     9241 aggtgtggga ttgctttatc aagggctgac aattcgggga attgttatga ttgcgataat
     9301 ggttgtattc agccttttat gcgcgtatat ctttgcgcgg caaatgacaa cgccgatcaa
     9361 agccttagcg gacagtgcga ataaaatggc aaacctgaaa gatgtaccgc cgccgctgga
     9421 gcgaaaggat gagcttggcg cactggctca tgatatgcat tccatgtatg tcaggctgaa
     9481 agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa catgagttgg aggaaacaca
     9541 gcgatatttc tttgcggcag cttctcatga gctaaaaacg cccatcgcgg ctacaagcgt
     9601 tctgttggag ggaatgcttg aaaatatcgg tgactacaaa gatcattcta agtatctgcg
     9661 cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt tccgaaatac tggagcttgt
     9721 cagcctgaat gatgggagaa tcgtacccat agctgaaccg ttggacatag ggcgcacggt
     9781 tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca aacaaccagc ggttcgtcac
     9841 agatattcca gccgggcaaa ttgtcctgtc cgatccgagg ctgctccaaa aggcactatc
     9901 caatgtcata ttgaatgcag ttcagaacac gccgcaggga ggcgaggtac ggatatggag
     9961 tgagcctggt gctgaaaaat gccgcctttt tgttttgaac atgggcgttc acattgatga
    10021 tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt gatcaggcgc gaagcagaaa
    10081 aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg ccatgagcct
    10141 ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt taccgctcac
    10201 atcaacattg taaatattta aaacttaaat gattttgggc tctttgtcaa ataggactga
    10261 tagagtgaga taagaa
//
LOCUS       DACCXW010000337        10013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.105, whole genome shotgun sequence.
ACCESSION   DACCXW010000337 DACCXW010000000
VERSION     DACCXW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.105"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(173..1045)
                     /locus_tag="IVY71_003100"
     CDS             complement(173..1045)
                     /locus_tag="IVY71_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7987057.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1173..1742
                     /locus_tag="IVY71_003101"
     CDS             1173..1742
                     /locus_tag="IVY71_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7987058.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1809..2546
                     /locus_tag="IVY71_003102"
     CDS             1809..2546
                     /locus_tag="IVY71_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7987059.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2777..4045
                     /locus_tag="IVY71_003103"
     CDS             2777..4045
                     /locus_tag="IVY71_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7987060.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4532..4996
                     /locus_tag="IVY71_003104"
     CDS             4532..4996
                     /locus_tag="IVY71_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7987061.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4980..5609
                     /locus_tag="IVY71_003105"
     CDS             4980..5609
                     /locus_tag="IVY71_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7987062.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5963..7441
                     /gene="msr"
                     /locus_tag="IVY71_003106"
     CDS             5963..7441
                     /gene="msr"
                     /locus_tag="IVY71_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7987063.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7488..7934
                     /locus_tag="IVY71_003107"
     CDS             7488..7934
                     /locus_tag="IVY71_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7987064.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8133..8648)
                     /locus_tag="IVY71_003108"
     CDS             complement(8133..8648)
                     /locus_tag="IVY71_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7987065.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8765..9967
                     /locus_tag="IVY71_003109"
     CDS             8765..9967
                     /locus_tag="IVY71_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7987066.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3195 a         1751 c         2077 g         2990 t
ORIGIN      
        1 tatatagaca gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt
       61 atcagaaata aaaggaagga acaaattaag aaacttatgt tatttaatca ttggatcttt
      121 tgcttaactt attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt
      181 atttctccgt taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt
      241 tccaaatgag attcagtgaa gcttgtaatg gcggatcgag tgggatgaat actttttccg
      301 tcccataagt attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca
      361 tcaaagaagc attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga
      421 aacctagcca ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa
      481 cagttttcag ttcctgcgga tggatgactg tttgctttgc taaaggatga ttccgatgta
      541 tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg
      601 tcggttcgat tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat
      661 ctgcattccc gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa
      721 ggctatcaga aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag
      781 agatgattgc gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt
      841 ttccttttga ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagcctcgag
      901 tgaaaagtgt cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt
      961 gcgtgatatg cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa
     1021 aatacgtcag tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta
     1081 agtatagtta aaaggaatag ttaagtatgt tttataagta ttagacatca taagccgtcc
     1141 gttataaagt aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg
     1201 tcaataaaga gattcgagta acggatccgc tctttgaagt gatccatcag atccaagcgg
     1261 aaaacgaaga gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca
     1321 aacggctaga aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt
     1381 tttatacaga tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag
     1441 tgatgttcgt tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg
     1501 tcaacctcat cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa
     1561 agccaatcca catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg
     1621 taacgattgg cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag
     1681 acaataccat cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt
     1741 gaacagtaag atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg
     1801 agaatagaat gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa
     1861 tcggtgaagc aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac
     1921 gccgagaaga tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc
     1981 aaacagcaga tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa
     2041 agttcggtcg aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag
     2101 ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa
     2161 atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa
     2221 ctgattctgt ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat
     2281 ttgctgtccg tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat
     2341 ctacgatcat ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa
     2401 aggctgctct tgaacttcat gaagcgcaaa aagaatgggg attaacatct gaagatatag
     2461 cttctgctgt agcatttgcg atagaaacgc cagaccggat gtctgtcagc aacatgatta
     2521 tccgtccgac gacacaggaa gtataatcca taacggataa tatccagaga gaattacttg
     2581 ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat
     2641 ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag aaacaaatgt tatttaaata
     2701 tggacattga cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat
     2761 tcaaaggaga aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa
     2821 tctagcagtt gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca
     2881 gacatcgagt tcttcagaaa caggaattga aaacgataca gaaagtactt tgcagtcagc
     2941 tactgtctca gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac
     3001 acaaaccgaa gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat
     3061 cacgaaccaa ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga
     3121 attcgaaaac tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc
     3181 gttcattgat agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc
     3241 atcagtcatg attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc
     3301 tgcccctaat tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat
     3361 gcctacttcg gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata
     3421 tccttcgtac aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca
     3481 gccaggcgtt tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc
     3541 tacggcttgg ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa
     3601 tgtaatcgaa acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat
     3661 gtatcgtctg tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa
     3721 aaattatctg ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag
     3781 cggacaacct gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc
     3841 acaatctgag atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta
     3901 ttctggtggt tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca
     3961 tcactatacc ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga
     4021 aggaatagga ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta
     4081 gtaagaacag ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg
     4141 cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa
     4201 cttttttatt actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca
     4261 aaaggcgatt tcggaaattt ttggcggtgt aaaattatct actgcagaag agctatcttc
     4321 tatagtttga taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt
     4381 ccgttcattc tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta
     4441 ttacacagct gattctttga cttttttctt tttttattct attattgaca agtcaatcgt
     4501 tgacgagtca attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt
     4561 atatcaaatc aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat
     4621 aagtcttact agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag
     4681 cagtttgcaa gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt
     4741 ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt
     4801 agcgctaaca gaaaaagccc agaagaaaat caagcattgt gaaatggacc ataaagatac
     4861 aggttccgtg ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt
     4921 agataaattg aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa
     4981 tggaacaaaa aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg
     5041 atgaaacata caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg
     5101 caccttcttc tgtcaatatg aaaccatggc gctttgtggt cgctgaaagc gaggaagcaa
     5161 aagaaatctt gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa
     5221 tggtcatgat ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tacaaccaag
     5281 cttacgaatc agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc
     5341 cttattacaa gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct
     5401 tagcggctat gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg
     5461 gtggattcga agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc
     5521 cagttattat tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag
     5581 caccagaaaa gatcactatg ttcgaataaa aggttttcag ttactattca gtagctattg
     5641 ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc
     5701 gatgaaatta cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa
     5761 acaacaattt catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag
     5821 ctatgacaga tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat
     5881 gcagtttttt tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac
     5941 atgaaatagg agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt
     6001 cagctttggc aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg
     6061 tatagggatc ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg
     6121 tgaacttttt cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca
     6181 attaacctat ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt
     6241 aagactggaa gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt
     6301 gatacgtctt ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct
     6361 agatagaaaa agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt
     6421 ttttgttagt catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa
     6481 agacggagaa atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt
     6541 ggagaaaaag actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga
     6601 aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa
     6661 gaaaaagcaa caaatcagac tggatcggtt gtcttcctct aaacaaaaag acagtgtaca
     6721 aaaagccatc caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac
     6781 aaccaagccg cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca
     6841 cagccgctat cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact
     6901 ggtaaatggt gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg
     6961 ttcaggtaag acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc
     7021 tccgaaagta agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc
     7081 catcattcgt tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct
     7141 gaatcactta gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg
     7201 agaagcgacc cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt
     7261 agatgaaccg actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca
     7321 aatatatccg ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc
     7381 tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta
     7441 aatgtgctac gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata
     7501 aaaaagaagg aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg
     7561 cgtataattt atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc
     7621 ctggattcgt ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa
     7681 ttgcctttaa actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat
     7741 gcttgatggt tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag
     7801 gaatacctga tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg
     7861 ctttaccgtt gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc
     7921 aaagtaatga atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat
     7981 agtctcttga atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt
     8041 tcggatcgct ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca
     8101 atagatattt ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct
     8161 cttacttgct gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca
     8221 acataacgtc caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt
     8281 ttttgcatga ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt
     8341 ttatcgagcg tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg
     8401 ggagtttcct ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag
     8461 atcttttttt gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac
     8521 ttggcaagcg ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc
     8581 ttcaattcat acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa
     8641 gtattcataa aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc
     8701 acgttttaca tgtgcatatt gacatgtaaa ataaatcatg tatgatgcta atagagagaa
     8761 agggatggaa atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa
     8821 ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg
     8881 tctagtagac ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac
     8941 gaccctgtta tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt
     9001 cttatctagt cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt
     9061 attcgctggt cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg
     9121 ctggggtgtc gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt
     9181 agttggtaac acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc
     9241 tgttggccct ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg
     9301 ggtagcattt ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga
     9361 gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact
     9421 tcgttcagtt ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta
     9481 tgcgccattt ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg
     9541 gggtgtgctt ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac
     9601 gacttatcgg acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg
     9661 agctggaact tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca
     9721 aggaattatc aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa
     9781 cgttgcttcg tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat
     9841 tgcaggtgtg attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat
     9901 tgttcttatc agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga
     9961 agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DACCXW010000338         8154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.123, whole genome shotgun sequence.
ACCESSION   DACCXW010000338 DACCXW010000000
VERSION     DACCXW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.123"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            411..707
                     /locus_tag="IVY71_003110"
     CDS             411..707
                     /locus_tag="IVY71_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP7987067.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            708..1124
                     /locus_tag="IVY71_003111"
     CDS             708..1124
                     /locus_tag="IVY71_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7987068.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            1144..2688
                     /locus_tag="IVY71_003112"
     CDS             1144..2688
                     /locus_tag="IVY71_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7987069.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            2831..3055
                     /locus_tag="IVY71_003113"
     CDS             2831..3055
                     /locus_tag="IVY71_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7987070.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            3070..3939
                     /locus_tag="IVY71_003114"
     CDS             3070..3939
                     /locus_tag="IVY71_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7987071.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3920..4654
                     /locus_tag="IVY71_003115"
     CDS             3920..4654
                     /locus_tag="IVY71_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7987072.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4687..5595
                     /locus_tag="IVY71_003116"
     CDS             4687..5595
                     /locus_tag="IVY71_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7987073.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5592..6134
                     /gene="sat4"
                     /locus_tag="IVY71_003117"
     CDS             5592..6134
                     /gene="sat4"
                     /locus_tag="IVY71_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP7987074.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            6227..7021
                     /locus_tag="IVY71_003118"
                     /note="aph(3')-IIIa"
     CDS             6227..7021
                     /locus_tag="IVY71_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7987075.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(7129..7317)
                     /locus_tag="IVY71_003119"
                     /pseudo
     CDS             complement(7129..7317)
                     /locus_tag="IVY71_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7297..7869
                     /locus_tag="IVY71_003120"
     CDS             7297..7869
                     /locus_tag="IVY71_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7987076.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         2841 a         1288 c         1813 g         2212 t
ORIGIN      
        1 ctttctgatt tagcggacaa atactttgac ctaatctcag aagatataaa agaaaaaata
       61 ataaaatatc cgatttatac tttgtataaa gaactaaagt agttagcaaa tcatttacag
      121 caaatcacta tgttccacat accattttac atacttacct tatccacgac acatgttgag
      181 atagtatcga agataaagaa agttgctcca tgttgttttt gcgagatggg gaaatcaaag
      241 ccacattgac aaccatgatg aataagcttg ctttcagtca taagctaatc ctagaaccac
      301 ttttcaagtc agttagccaa atcgatgaag aaagcgaccg tgaaagaatg gacgctattg
      361 ataagctaat ggagcaacta ttggaagaac gcaataccct tattgcactg atgtccaaag
      421 gtttcctaga acctgctctt tttaatcagg aacgaaatgt tttagatagt gagattaaaa
      481 atcttactac cgaaaaaaca aacctggtaa cgaactctgc gagtggggtt ttgcgagcaa
      541 acgagataaa ggacctcatt aattatgtgt cagcagataa ttttaatggt gactacacgg
      601 aagaactatt tgaagaattt gtagtgaaca tcattgtaaa ttccagggat gagctgacat
      661 tcaatttgaa atgcggtctt tccctgaaag aaaaggtggt gagataaatg gcatatattc
      721 catatggata caaaattcaa gatggagtgg ttactgtcga tgaaaaggca gcaggtcaag
      781 taaaggtatt ctttgagaaa tacatatcag gactatccct tacagtggct ggcgaacagg
      841 caggtattga aaagacacat tctgtgatgg ggcgcatttt gaaaaacgtc aactaccttg
      901 gaaatgatac gtatccagca attattgata aagagatatt tgataaagct gaagaagtta
      961 gagataaacg tgcaaaggat ttaggacgag tggtagagct tgccgctttc acctctcccc
     1021 ctcccaaaga acgattcaaa atgaaaaagg cagataataa gatgccagtg gatccttttg
     1081 aacgagcaga atacttatac agtctgatag aaagcgagga ataaagtgac agagaaaaat
     1141 ataatggtta ttcctgctcg taaaagagta ggaagtacag ccgcaaaaga aaagataaag
     1201 aaacttcgtg ttgctgccta ttgccgtgtt tctacagaaa cagaagaaca aaattctagc
     1261 tatgaggttc aggtctcaca ctatacagag tttataaaga aaaataatga atgggagttt
     1321 gctggcatct ttgcagatga tggtatctct ggtacgaaca ctaaaaagcg tgacgaattt
     1381 aatcgcatga ttgcagagtg tatggacggt aacatcgaca tggttattac aaaatccatc
     1441 agccgatttg cgcgtaacac cctagactgc cttcaataca ttagacagct caaggataag
     1501 aacatatcag tttattttga gaaagagaac atcaacacca cggatgccaa aggtgaggtt
     1561 ttgctgacta ttatggcatc tttggcacag caggaaagcc agagcctttc acaaaacgtt
     1621 aagcttgggc tacagtaccg ataccaacaa ggaaaggtgc aggtcaacca taagcgattt
     1681 atgggctaca cgaaagatga agatggaaat ttaactattg tccccgaaga agctgagatt
     1741 atcaaacgca tctaccgaga ataccttgag ggtcagagtc tagtgggcat tggtcgagcc
     1801 cttgaaaagg atggtatttt aacagcagcg ggaaaaccaa gatggcgacc agaatcagtt
     1861 aagaagatac ttcaaaacga aaaatacatc ggagatgccc ttctgcaaaa gactgtcaca
     1921 gtagattttc tgaccaagaa acgagtgaaa aatgaagggc atgttcccca atattatgtt
     1981 gaaaatagcc atgaagcgat tattcctaaa gagttatttt tgcaggcgca agaagaaatt
     2041 catcggagaa gcaatatcta cacaggagaa ggcaagaaca aaagaattta cagtagcaag
     2101 tacgctttaa gtgccatcac cttctgtgga gattgtggcg atatttatag acgggtctat
     2161 tggaatatcc acggaagaaa agaatttgtc tggcgatgcg tgactagaat cgagcaaggt
     2221 cctgaagttt gtaagaaccg aaccgtaaaa gaagatgaac tttatggtgc agtaatgact
     2281 gcgattaata agctacttgc aggtgggaac aatatgataa agacactgga agagaatatt
     2341 catgctgtga ttggcgaaac cacagaatac caaatttcag agattaacaa ctcactagag
     2401 gaaaaacaaa aagaactaat caagctggcg aacaagggtc aagactatga ccacttagca
     2461 gatgagattg atgagctgag agacaagcga cagatccttt tagtagaaga tgcctccctc
     2521 agtggcgaga acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgc
     2581 accttagaat atgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa
     2641 ggccacttcg tcatagcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta
     2701 aatagcccat gactctacag tagagttgtg ggttttcttt tgcttgctga ttataataat
     2761 attttaataa ctattgtgtt tatcaaccaa atggtttata atattcttag cgtaaacttg
     2821 gaggtgctgt atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag
     2881 tgtttccgaa cttgctagac gtattggcca gactccacag aatttcaata aaaaattaca
     2941 acgagaaaca gtaaccttgg atgagttgaa ggccattgct gatgtgctag gtgtcaagtt
     3001 tgtgcaggca tttattttac cagatggaga agaaataaaa atatctaacg aataaaggag
     3061 gatgtttata tggtaggtaa tgttattcaa atagtaacag agaaattatc atctttgcct
     3121 tttatagaag gcatcgtatt agggggctca cgtgcaagag gcacccatac agaaaattct
     3181 gatatagata tcggaatcta ttacaattca gactcatttg acctgacagc gattaatcaa
     3241 attgctacag agttggatga tgagaataga aacaaccttg ttgtacctcc cggagcgtgg
     3301 ggtgattggg ttaatggcgg tggatggtta gttataaacg ggtgtcatgt tgacttgata
     3361 ttacgtgata taaaacgggt ggaacaaata atcaaagata cggagcaagg aattgttact
     3421 gccaattatc agaccgggca tccccatggt tatataagtg ctatgtatcg tggagaatta
     3481 gcgattagta aaatactata tgctaagaac gaaagcttat gcgaattaaa aaagcaggca
     3541 gaaatttacc ctactgctct aaagaaaagc ttgatgaact tttttatatt tgaagctgaa
     3601 ttctctttaa tgtttgtaaa agcaaatgcg ggggcagagg ataaatatta tattgcaggc
     3661 catgtttttc gtattatttc atgcttaaat caagtactat ttgcatgtaa taatgcttat
     3721 tgcattaacg aaaagaaagc tataaaactg cttgaaactt ttgaatataa acctgaaaaa
     3781 tatgccgaaa gagttaatca tatttttgaa gtactcggtc tttctctttt tgaatgctac
     3841 gacatgaccg agaaacttta taaagaagtg aatgaaattg tatcggagat aaataacttt
     3901 ttaaacgagg agagttcaga tgaaagaaaa caaatatgat gataatatat tttttcaaaa
     3961 atacagtcaa atgagtcgct cgcagaaagg actggctggt gcgggagaat gggagacttt
     4021 gaaaaagatg ctacctgatt ttaagggtaa gcgtgtgctt gatttaggat gcggctatgg
     4081 atggcactgt atatatgcga tggaaaacgg tgcttcctct gtagtaggtg ttgatatttc
     4141 tcataaaatg ctcgaagtag caaaaggaaa aacccatttt ccacagattg aatatgaatg
     4201 ctgtgccata gaagatgtgg atttcccaga ggagagcttt gatgtaatac taagttcgct
     4261 tgcgtttcat tatgtagcag actatgagaa tttaataaaa aagatatata ggatgctgaa
     4321 ggctggtggc aatttagttt ttacagttga acatcctgtt tttactgctc atggaacaca
     4381 agactggtat tataacgaaa aaggagaaat actgcatttc ccggtggaca attattatta
     4441 tgagggcaaa cggacagcta tgtttttgga agaaaaggtt acaaaatatc atagaacact
     4501 gaccacatat ctaaatacac tgctttcaaa tagttttata ataaatcaga ttgtggagcc
     4561 acagccgcca gagaacatga tggatattcc ggggatggcg gatgaaatgc gacgcccaat
     4621 gatgctgatt gtatcggcaa aaaagaagat gtaataatat agaaaaaata aacgaggagt
     4681 atgtaaatga gatcagaaaa agaaatgatg gatttagtac tttctttagc agaacaggat
     4741 gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat
     4801 gaatttcagg attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat
     4861 gatgactggc ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa
     4921 ttattcccac ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa
     4981 attgatctta ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta
     5041 ataaaggttc taattgataa agattgtaga attaaaaggg acatagttcc gactgatata
     5101 gattatcatg taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg
     5161 aatgtaacac cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat
     5221 cattttaatc agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc
     5281 gaaacaggct ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc
     5341 gaggatttgt gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg
     5401 gaagcattat ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg
     5461 cttcattatg cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat
     5521 aaaaaataca ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag
     5581 aggcgggaac agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca
     5641 gcgaaccatt tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct
     5701 ttacagaatt actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag
     5761 aggatgagga ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt
     5821 actaccaaga cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg
     5881 cttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc
     5941 ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa
     6001 cccaggacaa taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct
     6061 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
     6121 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt
     6181 cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag
     6241 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
     6301 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
     6361 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
     6421 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
     6481 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
     6541 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
     6601 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
     6661 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt
     6721 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
     6781 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
     6841 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
     6901 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
     6961 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
     7021 gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt
     7081 tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc
     7141 tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc
     7201 ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat
     7261 atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc
     7321 tctattctta ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga
     7381 tttaatataa gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata
     7441 ccaattggat ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg
     7501 gtaatgttta cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc
     7561 catggagatg aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt
     7621 ttgaagcatg gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc
     7681 acatctggac agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc
     7741 tgcaataaaa gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc
     7801 agaatgatag agcctatatc actggggttt tatgaacaga actggtactt aatcggtttt
     7861 gcaggctaag aaatgaatat aggaattttc gtgtggatcg gattaaaagc atttgcatag
     7921 aagaagagct ctgtcaaagc catgatggct ctttggaaca aatattaaaa caaatgcttt
     7981 catataaaaa actatatgcc ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt
     8041 gattttatat ctttctcaaa ataaatataa aagcccttta aagagggctt ttatatatta
     8101 atcacaaatc acttatcaca aatcacaagt gattaatcac aaatcacttg tgat
//
LOCUS       DACCXW010000339         7978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.125, whole genome shotgun sequence.
ACCESSION   DACCXW010000339 DACCXW010000000
VERSION     DACCXW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.125"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..147)
                     /locus_tag="IVY71_003121"
     CDS             complement(<1..147)
                     /locus_tag="IVY71_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7987077.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFN"
     gene            273..1394
                     /locus_tag="IVY71_003122"
     CDS             273..1394
                     /locus_tag="IVY71_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7987078.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1652..3154
                     /locus_tag="IVY71_003123"
     CDS             1652..3154
                     /locus_tag="IVY71_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7987079.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3221..3748
                     /locus_tag="IVY71_003124"
     CDS             3221..3748
                     /locus_tag="IVY71_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7987080.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3959..5020
                     /locus_tag="IVY71_003125"
     CDS             3959..5020
                     /locus_tag="IVY71_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7987081.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            5023..5919
                     /gene="murQ"
                     /locus_tag="IVY71_003126"
     CDS             5023..5919
                     /gene="murQ"
                     /locus_tag="IVY71_003126"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7987082.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5933..7915
                     /locus_tag="IVY71_003127"
     CDS             5933..7915
                     /locus_tag="IVY71_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7987083.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
BASE COUNT         2659 a         1403 c         1806 g         2110 t
ORIGIN      
        1 gttaaagtca gcaatcaacc catttgtacg aatggcttca atgtgagcag gccaacgatc
       61 gatcaagagg acctcatttc cgctttgatg tagcatcaat ccgattcggc tgcccattgc
      121 tccggcccca gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt
      181 ttagtatata gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag
      241 tgtcttactt aagaagaaag tgagggatac agatggatat tttggcggca attgattcat
      301 ttaaaggaag tgcttcttca tttgaactaa atcaggcagt tctaaatggc tgggaagatc
      361 gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa
      421 tctattacgc gctcagggga acatggcgta ccattgatgg gatggatctc ttattccgtc
      481 agcgaaaaat cagctattta ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat
      541 ctgcacaagc attggggtta gatctacttg ataaaccaac ggatcaaact atccgacttg
      601 cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa
      661 ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct
      721 tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc
      781 attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta
      841 ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
      901 tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag
      961 atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg
     1021 gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca
     1081 ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg
     1141 atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc
     1201 gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt
     1261 ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac
     1321 acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca
     1381 tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga
     1441 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
     1501 gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga
     1561 tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata
     1621 ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac
     1681 attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac
     1741 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
     1801 tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt
     1861 cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga
     1921 ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt
     1981 atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt
     2041 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     2101 tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag
     2161 ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca
     2221 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     2281 atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc
     2341 agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg
     2401 attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg
     2461 gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga
     2521 aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca
     2581 ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga
     2641 gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc
     2701 aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga
     2761 aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg
     2821 aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa
     2881 cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat
     2941 gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac
     3001 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     3061 cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt
     3121 atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg
     3181 tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca
     3241 cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac
     3301 tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc
     3361 aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc
     3421 tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga
     3481 ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt
     3541 acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa
     3601 agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag
     3661 agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga
     3721 taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt
     3781 caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata
     3841 taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt
     3901 ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga gggataatat
     3961 gtttggcttt tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg
     4021 ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga
     4081 tgtttcggca tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact
     4141 agaattgatg gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga
     4201 tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa
     4261 tcagcagatt gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac
     4321 gcctaaagat atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg
     4381 gcataattat tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa
     4441 tcaatggctc aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt
     4501 gagaggccca ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct
     4561 tgcaggcgct ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg
     4621 tctgtcagaa aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct
     4681 ccatacggaa cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg
     4741 gcaagacgat gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc
     4801 tatcccagca agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa
     4861 gtatttgcgc tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa
     4921 agtgaatgta gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc
     4981 tggtatgaac tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta
     5041 gagaacctaa caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt
     5101 tctcaagccg ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa
     5161 gaactgacaa gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga
     5221 cgtctgattt atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa
     5281 tgcgtaccga cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa
     5341 gaagcaatga ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat
     5401 ctaatcaagc tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt
     5461 acaccatatg tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg
     5521 atttcttgca ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat
     5581 gcaggtccag aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt
     5641 atcttgaata tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg
     5701 atggtcgatg tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg
     5761 caagcgacag aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat
     5821 gttaagctgg ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa
     5881 ttagctgaag caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa
     5941 aaacgaactg actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg
     6001 caatgtagaa agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga
     6061 tcttgtaaat gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga
     6121 acagcttcaa gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga
     6181 tttggcaggc gtgaaattag gagaaaaact ttcttctgta agtacagcta cagcaaattc
     6241 atcagcatct ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa
     6301 atcgaaacaa aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt
     6361 gccaatgatt cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc
     6421 aaacttagtc acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat
     6481 gaacatcttg aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc
     6541 acaagttttt ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac
     6601 agggatgaat ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg
     6661 acaaggtggg atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa
     6721 actgcataaa gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcattgat
     6781 tgccatcggt ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg
     6841 catggtcggt gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct
     6901 aggcacatta ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca
     6961 tgtgcaaatg attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg
     7021 tgcaggacaa gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt
     7081 aacagaaatg atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat
     7141 ttacggagta acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat
     7201 cggcggggca gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg
     7261 agccgctttg atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt
     7321 ggcagcttat gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt
     7381 agaaaaagaa gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt
     7441 acctcaaact tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt
     7501 ttctttagat aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt
     7561 tgtagaacca gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt
     7621 cccaacgaaa cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat
     7681 gggaatcaac acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca
     7741 gcaagtgaca cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg
     7801 caaagaaaca tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt
     7861 ggaaaaaaca ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga
     7921 tagaaacaat agaaacgaaa gaagaaactg aaatctattt ttcagcttct tctttttt
//
LOCUS       DACCXW010000340         7943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.127, whole genome shotgun sequence.
ACCESSION   DACCXW010000340 DACCXW010000000
VERSION     DACCXW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.127"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            128..1564
                     /locus_tag="IVY71_003128"
     CDS             128..1564
                     /locus_tag="IVY71_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7987084.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4185
                     /locus_tag="IVY71_003129"
     CDS             1753..4185
                     /locus_tag="IVY71_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7987085.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            4415..5482
                     /gene="rlmN"
                     /locus_tag="IVY71_003130"
     CDS             4415..5482
                     /gene="rlmN"
                     /locus_tag="IVY71_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7987086.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5675..6778)
                     /locus_tag="IVY71_003131"
     CDS             complement(5675..6778)
                     /locus_tag="IVY71_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7987087.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7330..7878
                     /gene="aac(6')"
                     /locus_tag="IVY71_003132"
     CDS             7330..7878
                     /gene="aac(6')"
                     /locus_tag="IVY71_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7987088.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2658 a         1357 c         1724 g         2204 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaagttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     2521 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     2581 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     2641 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     2701 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     2761 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     2821 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     2881 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     2941 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     3001 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     3061 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     3121 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     3181 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     3241 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     3301 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     3361 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     3421 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     3481 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     3541 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     3601 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     3661 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     3721 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     3781 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     3841 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     3901 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     3961 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     4021 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     4081 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     4141 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     4201 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     4261 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     4321 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     4381 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     4441 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     4501 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     4561 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     4621 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     4681 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     4741 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     4801 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     4861 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     4921 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     4981 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     5041 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     5101 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     5161 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     5221 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     5281 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     5341 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     5401 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     5461 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     5521 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     5581 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     5641 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     5701 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     5761 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     5821 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     5881 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     5941 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     6001 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     6061 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     6121 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     6181 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     6241 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     6301 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     6361 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     6421 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     6481 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
     6541 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
     6601 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
     6661 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
     6721 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
     6781 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
     6841 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
     6901 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
     6961 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
     7021 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
     7081 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
     7141 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
     7201 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
     7261 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
     7321 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
     7381 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
     7441 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
     7501 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
     7561 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
     7621 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
     7681 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
     7741 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
     7801 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
     7861 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
     7921 gaaggaattt acgaaaatta ctt
//
LOCUS       DACCXW010000341         4819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.166, whole genome shotgun sequence.
ACCESSION   DACCXW010000341 DACCXW010000000
VERSION     DACCXW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.166"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            191..997
                     /locus_tag="IVY71_003133"
     CDS             191..997
                     /locus_tag="IVY71_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP7987089.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1015..1842
                     /gene="vanW"
                     /locus_tag="IVY71_003134"
     CDS             1015..1842
                     /gene="vanW"
                     /locus_tag="IVY71_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7987090.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1839..2810
                     /locus_tag="IVY71_003135"
     CDS             1839..2810
                     /locus_tag="IVY71_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7987091.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2803..3831
                     /gene="vanB"
                     /locus_tag="IVY71_003136"
     CDS             2803..3831
                     /gene="vanB"
                     /locus_tag="IVY71_003136"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7987092.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVGKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3837..4445
                     /locus_tag="IVY71_003137"
     CDS             3837..4445
                     /locus_tag="IVY71_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7987093.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1360 a         1016 c         1277 g         1166 t
ORIGIN      
        1 agaatgattt tctatcagtc ctagaaatta tagagccgat tttgaccgac aggtataacc
       61 ctgccggtct ttttttgctg ctacaggaaa actacagact ggctacaggg aaagtacaga
      121 tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt agttttattg caaaggaggc
      181 attcgatcaa atggaaaaaa gcaactatca ttccaatgcg gatcatcaca aacggcatat
      241 gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc attatttcgt tcacagtctg
      301 cacgctgttt ttggggtgga gactggcttc cgtattggag gcaacacaga taccgcccat
      361 ccctgcaact catacaggca gcagcactga cgtagtggag aatttggagg aaaacgctct
      421 tgccaccgcc aaagaacagg gagatgaaca ggaatggagc ctgattttag tgaacaggca
      481 gaaccccatc cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat
      541 agatattcgg atttctccct atcttcaaga tttgtttgat gccgcaagaa ctgatggagt
      601 ttacccgatt gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa ttatggatga
      661 aaaaattgcc gaatataagg cgaaaggcta cacctctgca caggctaaag cggaagcaga
      721 aacttgggtg gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg tggatatcaa
      781 tgcggacgga attcattcaa caggcaacga ggtttataga tggctggatg aaaacagcta
      841 tcgttttggt tttattcgtc gctacccacc agacaagaca gagataaccg gtgtgagcaa
      901 cgagccgtgg cattaccgat atgttggcat cgaagctgcc acagagatgt acaaccaagg
      961 ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa aaggatgtta tgttatggac
     1021 agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc gcagagaaaa
     1081 atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac gataggggaa
     1141 aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca caataccgga
     1201 tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc aaagacctta
     1261 aacggcttat tgataagacc gggggaaacc ttttctttct ggtggctggt acgccatgcg
     1321 gacaaagata ccccctataa agacggcctt acggtgacca atggtaaact caccaccatg
     1381 tcgggcggcg gtatgtgcca gatgagcaat ttactatttt ggatgttcct gcatacgcca
     1441 ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc aaacagtgac
     1501 gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa agtgcgaaac
     1561 gataccgact gcacctacca aatatgggtg accttagatg atgagaaaat catcggtcag
     1621 gtgttcgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag tatccagtat
     1681 gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca agttgcctta
     1741 ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa aatctgttat
     1801 cccctcccgg aaagtgtgga tattcaggag gagaaccaat gagaaaaagt gtgggcatta
     1861 ctgtttttgg atgcgaacag gatgaggcaa atgctttccg cgctttatcg ccggattttc
     1921 atattatccc tacgctgatt agcgatgcga tatcggcaga caacgcaaaa ttggccgctg
     1981 gcaatcaatg cgttagcgta agccataagt ccgaggtttc cgaggcgaca attcttgcgc
     2041 tgagaaaggt cggggtaaaa tacatttcta cccgcagcat cggctgcgat cacattgata
     2101 cgactgccgc cgagagaatg ggaatctcgg ttggcacggt tgcgtattcg ccggacagcg
     2161 ttgcggatta tgctttgatg ctgatgctga tggccatacg gggtgcaaaa cccaccatgc
     2221 acgccgtggc gcaacaagat ttcagattgg atcgtatccg ggggaaagaa ctgggggata
     2281 tgactgtggg agttattgga accggccata tcgggcaagc ggtcgtcaaa aggctgcggg
     2341 gatttggatg ccatgtgctg gcctatgata acagccgaaa aatggatgca gattatgtcc
     2401 agcttgatga gcttctaaaa aacagcgata ttgttacgct ccatgtgccg ctttgtgcgg
     2461 atacccgcca tctgatcggt cagaagcaaa ttggagagat gaagcaaggc gcatttttaa
     2521 tcaacaccgg gcgcggagcg cttgtcgata ccggggcgct ggtggaggcg ttggaaagcg
     2581 gaaagctggg tggtgcggca ctggatgtgt tggagggcga ggatcagttt gtttataccg
     2641 attgctcgca gaaagtgctt gaccatccct ttttgtcgca gcttttaagg atgccgaatg
     2701 tgatcatcac accccatacg gcgtactaca ctgagcgtgt gctgcaggat actacagaaa
     2761 aaacaatcag gaattgtctc aattttgaaa ggagtttaca gcatgaatag aataaaagtc
     2821 gcaatcatct tcggcggttg ctcggaggaa catgatgtgt cggtaaaagc cgcaatagaa
     2881 attgctgcga acattgatac ggaaaaattc gatccgcact acatcggaat tacaaaaaac
     2941 ggtgtaggga agctatgcaa gaagccatgt acggaatggg aagccgacag tctccccgcc
     3001 atactctccc cggataggaa aacgcatggg ctgcttgtca tgaaagaaag cgaatacgaa
     3061 acacggcgta ttgatgtggc tttcccggtt ttgcatggca aatgcgggga ggatggtgcg
     3121 atacaggggc tgtttgtatt gtctggtatc ccctatgtgg gctgtgatat tcaaagctcc
     3181 gcagcttgca tggacaaatc actggcctac attcttacaa aaaatgcggg catcgccgtt
     3241 cccgaatttc aaatgattga taaaggtgac aagccggagg cgggtgcgct tacctaccct
     3301 gtctttgtga agccggcacg gtcaggttcg tcctttggcg taaccaaagt aaacggtacg
     3361 gaagaactta acgctgcgat agaagcggca ggacaatatg atggaaaaat cttaattgag
     3421 caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg ggaacgagga tgatttgatt
     3481 gtcggcgaag tggatcaaat ccggctgagc cacggtatct tccgcatcca tcaggaaaac
     3541 gagccggaaa aaggctcaga aaatgcgatg attacagttc ccgcagacat tccggtcgag
     3601 gaacgaaatc gggtgcagga aacggcaaag aaagtatatc gggtgcttgg atgcagaggg
     3661 cttgcccgtg ttgatctttt tttgcaggag gatggcggca tcgttctaaa tgaggtcaat
     3721 accctgcccg gttttacatc gtacagccgc tacccacgta tgatggccgc cgcaggaatc
     3781 acgcttcctg cactgattga cagcctgatt acattggcgt taaagaggtg ataagtatgg
     3841 aaaatggttt tttgttttta gatgaaatgt tgcatggcgt ccgttgggat gccaagtacg
     3901 ccacatggga taacttcaca ggaaaaccgg tggatgggta tgaggtgaat cgcatcatcg
     3961 gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat ccatgcggct gcgcttggct
     4021 atggcttgct tttatgggat ggatatcggc caagaactgc ggtggactgc ttcctgcgtt
     4081 gggcagcgca accggaggac aatctcacaa aagaaaaatt ttaccccaat atagagcgag
     4141 ccgagttgat tacaaagggt tatgtggcct cacaatccag ccatagccgt ggaagcgcaa
     4201 ttgatcttac gctctaccac ctggatacag gggaacttgt ttcaatggga agtaacttcg
     4261 attttatgga cgaacggtcg catcatacag caaaagggat aggggatgca gaggcacaaa
     4321 atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt tcagtcttat cgctttgaat
     4381 ggtggcacta taagttgatt gatgagccat accccgatac ctattttaat tttgccgttt
     4441 cataatgaaa gtattggttt tgtatcagat tttggatttg ataaaatcta tgctggtcat
     4501 ggacaatctt ttactggaaa gtggttaaag catcttttgt aatacattgc aaattatagt
     4561 attcaatctg cccagcggca gaaaagaaaa aaaccgctgt tgggcagacc tttcccacgc
     4621 ccattttgta ttttgctggc ggcggttttg agaaatcaag gccgccgttc ttttctggca
     4681 gcaaaaggtg tggcggccca taggaggatg ccgctatgct gttttgctat tgccatgctc
     4741 taatccactt ccaccactca aaaaaagcct tgcccctcca tgaactgaac cccaaaagtt
     4801 gaactattta atggactgt
//
LOCUS       DACCXW010000342         4819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.167, whole genome shotgun sequence.
ACCESSION   DACCXW010000342 DACCXW010000000
VERSION     DACCXW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.167"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            191..997
                     /locus_tag="IVY71_003138"
     CDS             191..997
                     /locus_tag="IVY71_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP7987094.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1015..1842
                     /gene="vanW"
                     /locus_tag="IVY71_003139"
     CDS             1015..1842
                     /gene="vanW"
                     /locus_tag="IVY71_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7987095.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1839..2810
                     /locus_tag="IVY71_003140"
     CDS             1839..2810
                     /locus_tag="IVY71_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7987096.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2803..3831
                     /gene="vanB"
                     /locus_tag="IVY71_003141"
     CDS             2803..3831
                     /gene="vanB"
                     /locus_tag="IVY71_003141"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7987097.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3837..4445
                     /locus_tag="IVY71_003142"
     CDS             3837..4445
                     /locus_tag="IVY71_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7987098.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1360 a         1016 c         1276 g         1167 t
ORIGIN      
        1 agaatgattt tctatcagtc ctagaaatta tagagccgat tttgaccgac aggtataacc
       61 ctgccggtct ttttttgctg ctacaggaaa actacagact ggctacaggg aaagtacaga
      121 tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt agttttattg caaaggaggc
      181 attcgatcaa atggaaaaaa gcaactatca ttccaatgcg gatcatcaca aacggcatat
      241 gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc attatttcgt tcacagtctg
      301 cacgctgttt ttggggtgga gactggcttc cgtattggag gcaacacaga taccgcccat
      361 ccctgcaact catacaggca gcagcactga cgtagtggag aatttggagg aaaacgctct
      421 tgccaccgcc aaagaacagg gagatgaaca ggaatggagc ctgattttag tgaacaggca
      481 gaaccccatc cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat
      541 agatattcgg atttctccct atcttcaaga tttgtttgat gccgcaagaa ctgatggagt
      601 ttacccgatt gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa ttatggatga
      661 aaaaattgcc gaatataagg cgaaaggcta cacctctgca caggctaaag cggaagcaga
      721 aacttgggtg gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg tggatatcaa
      781 tgcggacgga attcattcaa caggcaacga ggtttataga tggctggatg aaaacagcta
      841 tcgttttggt tttattcgtc gctacccacc agacaagaca gagataaccg gtgtgagcaa
      901 cgagccgtgg cattaccgat atgttggcat cgaagctgcc acagagatgt acaaccaagg
      961 ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa aaggatgtta tgttatggac
     1021 agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc gcagagaaaa
     1081 atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac gataggggaa
     1141 aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca caataccgga
     1201 tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc aaagacctta
     1261 aacggcttat tgataagacc gggggaaacc ttttctttct ggtggctggt acgccatgcg
     1321 gacaaagata ccccctataa agacggcctt acggtgacca atggtaaact caccaccatg
     1381 tcgggcggcg gtatgtgcca gatgagcaat ttactatttt ggatgttcct gcatacgcca
     1441 ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc aaacagtgac
     1501 gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa agtgcgaaac
     1561 gataccgact gcacctacca aatatgggtg accttagatg atgagaaaat catcggtcag
     1621 gtgttcgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag tatccagtat
     1681 gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca agttgcctta
     1741 ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa aatctgttat
     1801 cccctcccgg aaagtgtgga tattcaggag gagaaccaat gagaaaaagt gtgggcatta
     1861 ctgtttttgg atgcgaacag gatgaggcaa atgctttccg cgctttatcg ccggattttc
     1921 atattatccc tacgctgatt agcgatgcga tatcggcaga caacgcaaaa ttggccgctg
     1981 gcaatcaatg cgttagcgta agccataagt ccgaggtttc cgaggcgaca attcttgcgc
     2041 tgagaaaggt cggggtaaaa tacatttcta cccgcagcat cggctgcgat cacattgata
     2101 cgactgccgc cgagagaatg ggaatctcgg ttggcacggt tgcgtattcg ccggacagcg
     2161 ttgcggatta tgctttgatg ctgatgctga tggccatacg gggtgcaaaa cccaccatgc
     2221 acgccgtggc gcaacaagat ttcagattgg atcgtatccg ggggaaagaa ctgggggata
     2281 tgactgtggg agttattgga accggccata tcgggcaagc ggtcgtcaaa aggctgcggg
     2341 gatttggatg ccatgtgctg gcctatgata acagccgaaa aatggatgca gattatgtcc
     2401 agcttgatga gcttctaaaa aacagcgata ttgttacgct ccatgtgccg ctttgtgcgg
     2461 atacccgcca tctgatcggt cagaagcaaa ttggagagat gaagcaaggc gcatttttaa
     2521 tcaacaccgg gcgcggagcg cttgtcgata ccggggcgct ggtggaggcg ttggaaagcg
     2581 gaaagctggg tggtgcggca ctggatgtgt tggagggcga ggatcagttt gtttataccg
     2641 attgctcgca gaaagtgctt gaccatccct ttttgtcgca gcttttaagg atgccgaatg
     2701 tgatcatcac accccatacg gcgtactaca ctgagcgtgt gctgcaggat actacagaaa
     2761 aaacaatcag gaattgtctc aattttgaaa ggagtttaca gcatgaatag aataaaagtc
     2821 gcaatcatct tcggcggttg ctcggaggaa catgatgtgt cggtaaaagc cgcaatagaa
     2881 attgctgcga acattgatac ggaaaaattc gatccgcact acatcggaat tacaaaaaac
     2941 ggtgtatgga agctatgcaa gaagccatgt acggaatggg aagccgacag tctccccgcc
     3001 atactctccc cggataggaa aacgcatggg ctgcttgtca tgaaagaaag cgaatacgaa
     3061 acacggcgta ttgatgtggc tttcccggtt ttgcatggca aatgcgggga ggatggtgcg
     3121 atacaggggc tgtttgtatt gtctggtatc ccctatgtgg gctgtgatat tcaaagctcc
     3181 gcagcttgca tggacaaatc actggcctac attcttacaa aaaatgcggg catcgccgtt
     3241 cccgaatttc aaatgattga taaaggtgac aagccggagg cgggtgcgct tacctaccct
     3301 gtctttgtga agccggcacg gtcaggttcg tcctttggcg taaccaaagt aaacggtacg
     3361 gaagaactta acgctgcgat agaagcggca ggacaatatg atggaaaaat cttaattgag
     3421 caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg ggaacgagga tgatttgatt
     3481 gtcggcgaag tggatcaaat ccggctgagc cacggtatct tccgcatcca tcaggaaaac
     3541 gagccggaaa aaggctcaga aaatgcgatg attacagttc ccgcagacat tccggtcgag
     3601 gaacgaaatc gggtgcagga aacggcaaag aaagtatatc gggtgcttgg atgcagaggg
     3661 cttgcccgtg ttgatctttt tttgcaggag gatggcggca tcgttctaaa tgaggtcaat
     3721 accctgcccg gttttacatc gtacagccgc tacccacgta tgatggccgc cgcaggaatc
     3781 acgcttcctg cactgattga cagcctgatt acattggcgt taaagaggtg ataagtatgg
     3841 aaaatggttt tttgttttta gatgaaatgt tgcatggcgt ccgttgggat gccaagtacg
     3901 ccacatggga taacttcaca ggaaaaccgg tggatgggta tgaggtgaat cgcatcatcg
     3961 gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat ccatgcggct gcgcttggct
     4021 atggcttgct tttatgggat ggatatcggc caagaactgc ggtggactgc ttcctgcgtt
     4081 gggcagcgca accggaggac aatctcacaa aagaaaaatt ttaccccaat atagagcgag
     4141 ccgagttgat tacaaagggt tatgtggcct cacaatccag ccatagccgt ggaagcgcaa
     4201 ttgatcttac gctctaccac ctggatacag gggaacttgt ttcaatggga agtaacttcg
     4261 attttatgga cgaacggtcg catcatacag caaaagggat aggggatgca gaggcacaaa
     4321 atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt tcagtcttat cgctttgaat
     4381 ggtggcacta taagttgatt gatgagccat accccgatac ctattttaat tttgccgttt
     4441 cataatgaaa gtattggttt tgtatcagat tttggatttg ataaaatcta tgctggtcat
     4501 ggacaatctt ttactggaaa gtggttaaag catcttttgt aatacattgc aaattatagt
     4561 attcaatctg cccagcggca gaaaagaaaa aaaccgctgt tgggcagacc tttcccacgc
     4621 ccattttgta ttttgctggc ggcggttttg agaaatcaag gccgccgttc ttttctggca
     4681 gcaaaaggtg tggcggccca taggaggatg ccgctatgct gttttgctat tgccatgctc
     4741 taatccactt ccaccactca aaaaaagcct tgcccctcca tgaactgaac cccaaaagtt
     4801 gaactattta atggactgt
//
LOCUS       DACCXW010000343         2771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.209, whole genome shotgun sequence.
ACCESSION   DACCXW010000343 DACCXW010000000
VERSION     DACCXW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.209"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            110..193
                     /locus_tag="IVY71_003143"
     CDS             110..193
                     /locus_tag="IVY71_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP7987099.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="IVY71_003144"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="IVY71_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7987100.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="IVY71_003145"
     CDS             complement(1318..2181)
                     /locus_tag="IVY71_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7987101.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="IVY71_003146"
     CDS             complement(2183..2455)
                     /locus_tag="IVY71_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7987102.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="IVY71_003147"
     CDS             complement(2472..2681)
                     /locus_tag="IVY71_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7987103.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          990 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattaa
     2761 tcacttgtga t
//
LOCUS       DACCXW010000344         1962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.234, whole genome shotgun sequence.
ACCESSION   DACCXW010000344 DACCXW010000000
VERSION     DACCXW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.234"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            180..872
                     /locus_tag="IVY71_003148"
     CDS             180..872
                     /locus_tag="IVY71_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7987104.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1199..1696
                     /gene="dfrG"
                     /locus_tag="IVY71_003149"
     CDS             1199..1696
                     /gene="dfrG"
                     /locus_tag="IVY71_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7987105.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1768..>1962)
                     /locus_tag="IVY71_003150"
     CDS             complement(1768..>1962)
                     /locus_tag="IVY71_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7987106.1"
                     /translation="RGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTP
                     AQRLGITDKQFDWKDIIYFK"
BASE COUNT          792 a          258 c          355 g          557 t
ORIGIN      
        1 gtattaacct gcatttttct ctataaaaaa gattttttaa atagataaaa aaggaggatt
       61 aaatgagtat ttgatagtat ttatattaaa attacatatg taatttaaaa acgcttgaca
      121 ttgtgtttga gatcacttac ttttagcatg taaaagtatt ggattaagga gctatgcaaa
      181 tgaaaaagaa atttattact ggtgggattg tcgttgcatt tctatgtcta attggctaca
      241 caggcacgcg gtacgcaaat gaaattattt tgtggggtgg agagacagat attgatacaa
      301 tcaatgagaa tctgacacag cttgatcata cattgacaga taaagagcaa gcgattacag
      361 atcttaccgc tcgactagca caaaaagaac agacattgca agattcaaag gcagatgtcc
      421 aagaatacca aacaaaagta aatgaattag aaaaccaaaa aaatcaatcg gcagcagaaa
      481 aacaaaactt agaaagtcaa ttagtcagca aggatgcaca actacaaagc aaacaaaatg
      541 aaatcaatgt caaactagat gagatcaacc aaaaaaatga tgaaatcaat catctgaatg
      601 cagattggac agcaagacta caagaagcac aagcaaaact ttcagaatta caaaatactg
      661 taaatggttt gaatcaacaa atcgcaacac tgacacaaga aaaaacaaat gtagaaactc
      721 agttaacaga tacacaaaag aaattagacg aagcaactgc tgaaaactca agcttgaaac
      781 aatatattca aaagttagaa aaagcaaaag atgaagtgga agatacagct gataaatctc
      841 agcaaattgt tgaagaacat ttgcgtaaat aatctttctt aacaagtaaa cctaaattag
      901 taaagaagca acagtaatct atactaagta gttcagtttt ggagtacaaa atgacaaaat
      961 gtacttcaaa atggactact tttttcattt taacttacat accaaattac atacaaaatg
     1021 atttataaaa tgcaattgaa aatgataatt gaatgaaaat ttgtgatttt gtggtattat
     1081 gataatataa cttatagaaa aaattgctta ttcaactaac gggcaggata atgagaaaag
     1141 gttttttgat agaaataaga aattagatgg taaattagtt aaaaaggctg tgttataaat
     1201 gaaagtttct ttgattgctg cgatggataa gaatagagtg attggcaaag agaatgacat
     1261 tccttggagg attcccaagg actgggaata tgttaaaaat actacaaagg gacatccgat
     1321 aatattaggt aggaagaacc ttgaatcaat cggaagagcc ttacctgaca gaagaaatat
     1381 tattctgacg agagataagg ggtttacctt taatggttgt gaaattgttc attcaataga
     1441 agatgttttt gagttatgta aaaacgaaga agaaattttt attttcggag gagaacagat
     1501 ttataatttg tttttccctt atgttgagaa aatgtacatc acaaaaatac atcatgaatt
     1561 cgaaggagat actttttttc cagaagtgaa ttatgaggaa tggaatgagg tatttgccca
     1621 aaaagggata aagaatgata aaaatccgta taactactat tttcatgtat atgaaagaaa
     1681 aaacttattg agttaacggg tgcgttaatt gaagaagaac agtaaagata aagcacaaga
     1741 gcttgggatt actccaagct ctttttgtca tttgaaataa attatatctt tccaatcaaa
     1801 ttgtttatct gtaatcccta atctttgagc aggtgtcaaa acctttttat ccgctgattt
     1861 aaaagggcga caaaagttgt agtaagttct tagaattgta atcgcaaatt gagcatactt
     1921 cggattgaag ttagcgtaaa tatagctctt cttatcacca cg
//
LOCUS       DACCXW010000345         1817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13710
            SAMN11027190-rid9896003.guided.241, whole genome shotgun sequence.
ACCESSION   DACCXW010000345 DACCXW010000000
VERSION     DACCXW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027190
            Sequence Read Archive: SRR10694460
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743213.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743213.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:02:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,087
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 26 of 62
            Pseudo Genes (incomplete)         :: 29 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 7 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027190-rid9896003.guided.241"
                     /isolate="UW13710"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            53..526
                     /gene="dfrF"
                     /locus_tag="IVY71_003151"
     CDS             53..526
                     /gene="dfrF"
                     /locus_tag="IVY71_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP7987107.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            653..1309
                     /locus_tag="IVY71_003152"
     CDS             653..1309
                     /locus_tag="IVY71_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP7987108.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            1491..>1817
                     /locus_tag="IVY71_003153"
     CDS             1491..>1817
                     /locus_tag="IVY71_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7987109.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGK"
BASE COUNT          679 a          210 c          373 g          555 t
ORIGIN      
        1 gtgcacttga tggaatcaca ggtacgacat catattatgg aggatgaaat agatgatagg
       61 tttgattgtt gcgaggtcaa agaataatgt tataggcaag aatggtaata taccatggaa
      121 aataaaggga gaacaaaagc aatttagaga gttaacaacg ggtaatgtgg ttattatggg
      181 gcgaaagtct tatgaagaaa tcggtcatcc gttgcctaat agaatgaata ttgttgtttc
      241 caccacaaca gagtatcaag gagataattt agtttcagtt aaatcattag aagatgcatt
      301 attattggct aaaggacgag atgtatacat atctggtgga tatggactat ttaaggaagc
      361 tttgcaaata gtagataaaa tgtatatcac agaagtagat ttaaatattg aagatggaga
      421 tacattcttt ccagaatttg atatcaatga ttttgaagtt ttgatagggg aaacacttgg
      481 tgaggaagtg aaatatacga gaacatttta tgtaaggaaa aaatgaattg agtaaatttt
      541 ggatttaggg tgttttcata aatatattgc tttttatgca tatataaatt gttgtaagac
      601 aaattccagt ttgtatgggg cgataaaatc agaatttgag agaggattga atatgaataa
      661 ggaatggtct gaacttaata aaacaatgca ggcacaaata aaaaagaaag atacttataa
      721 gaggggtatt gatactttac ttactttacg aagtcagtta atacaaacct tagtatcttt
      781 caaagaagaa ttatgcagag aagattttaa ctcaatcccc ttcataaacg ctgatggtta
      841 tcatagtaag acgattgctt attctatttg gcatattttc cgcattgaag atatagttgt
      901 gcatacagtg ataaatgaag atgaacaagt attttttgca ggcaattatc aagagcgtat
      961 caattcacct atcattacaa caggaaatga actcatgaaa cagcagattg cagacttttc
     1021 aaaacagctt aatctggaag aattatattt atatattttc gaagtatggg aaagcactga
     1081 gaaaatgctt gaacggttat cttatgatga attgaaaaga aaaataccga aagaaagaaa
     1141 aggatactta gaatcgttga atgtagtaaa cgacaatgaa aaagcaattt ggctgattga
     1201 ttattggtgt aataaagata tttgtggact aattcaaatg ccgttttcaa gacattggat
     1261 tatgcacaca gaagcctgtt tgcgtataaa gaacaagata cattcatagg caaaaatttc
     1321 agtttgtaga actaaggaaa ttggaattta aggaggaata aagtatggtt agtgatttta
     1381 taagagcagc atttccatgg attttaatgg gtttatttgt agcaatcagt tgttccctta
     1441 tgagtaaaaa agagaaataa attcctgttt gtatttataa aagagaaaaa atgagaaaag
     1501 tagttttatt tattgccatg agccttgacg gatatattgc ggacggtaat ggtggagtag
     1561 cttggctaaa tggtcatgga aatgacaatg aaaacatcga cacttataca gaatttacca
     1621 aagatataga tactgtctta atggggtgga atacttctca tcaggttgtt actgaacttt
     1681 ctccgcaaga atgggtatat aataaattta caacttatgt attaacacat aaagagtgca
     1741 attcttccca acaaattcat tttacaagtg aaaatcctgt tttattattg gaacggctaa
     1801 aacaagaagc aggaaag
//
